LOCUS       DADLXX010000001        85902 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLXX010000001 DADLXX010000000
VERSION     DADLXX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.001"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1592)
                     /locus_tag="JNX27_000001"
     CDS             complement(<1..1592)
                     /locus_tag="JNX27_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7881356.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="JNX27_000002"
     CDS             complement(1576..1932)
                     /locus_tag="JNX27_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7881357.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSTKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="JNX27_000003"
     CDS             complement(2055..2237)
                     /locus_tag="JNX27_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881358.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="JNX27_000004"
     CDS             complement(2221..2661)
                     /locus_tag="JNX27_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7881359.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="JNX27_000005"
     CDS             complement(2661..2957)
                     /locus_tag="JNX27_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7881360.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="JNX27_000006"
     CDS             complement(2954..3292)
                     /locus_tag="JNX27_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7881361.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="JNX27_000007"
     CDS             complement(3289..4500)
                     /locus_tag="JNX27_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7881362.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="JNX27_000008"
     CDS             complement(4502..5074)
                     /locus_tag="JNX27_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7881363.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="JNX27_000009"
     CDS             complement(5114..6271)
                     /locus_tag="JNX27_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7881364.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGNTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6574..6735)
                     /locus_tag="JNX27_000010"
     CDS             complement(6574..6735)
                     /locus_tag="JNX27_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7881365.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(6745..7050)
                     /locus_tag="JNX27_000011"
     CDS             complement(6745..7050)
                     /locus_tag="JNX27_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881366.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7047..7325)
                     /locus_tag="JNX27_000012"
     CDS             complement(7047..7325)
                     /locus_tag="JNX27_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881367.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7605..9353)
                     /locus_tag="JNX27_000013"
     CDS             complement(7605..9353)
                     /locus_tag="JNX27_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7881368.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9350..9649)
                     /locus_tag="JNX27_000014"
     CDS             complement(9350..9649)
                     /locus_tag="JNX27_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7881369.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9655..9888)
                     /locus_tag="JNX27_000015"
     CDS             complement(9655..9888)
                     /locus_tag="JNX27_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881370.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(9890..10110)
                     /locus_tag="JNX27_000016"
                     /pseudo
     CDS             complement(9890..10110)
                     /locus_tag="JNX27_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10103..10324)
                     /locus_tag="JNX27_000017"
     CDS             complement(10103..10324)
                     /locus_tag="JNX27_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881371.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10314..10514)
                     /locus_tag="JNX27_000018"
     CDS             complement(10314..10514)
                     /locus_tag="JNX27_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY7881372.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10523..11269)
                     /locus_tag="JNX27_000019"
     CDS             complement(10523..11269)
                     /locus_tag="JNX27_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881373.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11327..11515)
                     /locus_tag="JNX27_000020"
     CDS             complement(11327..11515)
                     /locus_tag="JNX27_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7881374.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(11890..13119)
                     /locus_tag="JNX27_000021"
     CDS             complement(11890..13119)
                     /locus_tag="JNX27_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7881375.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13368..14489
                     /locus_tag="JNX27_000022"
     CDS             13368..14489
                     /locus_tag="JNX27_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7881376.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="JNX27_000023"
     CDS             complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="JNX27_000023"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7881377.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16014..17150
                     /gene="potA"
                     /locus_tag="JNX27_000024"
     CDS             16014..17150
                     /gene="potA"
                     /locus_tag="JNX27_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7881378.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17134..17991
                     /gene="potB"
                     /locus_tag="JNX27_000025"
     CDS             17134..17991
                     /gene="potB"
                     /locus_tag="JNX27_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7881379.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17988..18782
                     /gene="potC"
                     /locus_tag="JNX27_000026"
     CDS             17988..18782
                     /gene="potC"
                     /locus_tag="JNX27_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7881380.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18779..19825
                     /gene="potD"
                     /locus_tag="JNX27_000027"
     CDS             18779..19825
                     /gene="potD"
                     /locus_tag="JNX27_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7881381.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSQLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="JNX27_000028"
     CDS             complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="JNX27_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7881382.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="JNX27_000029"
     CDS             complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="JNX27_000029"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7881383.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="JNX27_000030"
     CDS             complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="JNX27_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7881384.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="JNX27_000031"
     CDS             complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="JNX27_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7881385.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="JNX27_000032"
     CDS             complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="JNX27_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7881386.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25157..26203
                     /locus_tag="JNX27_000033"
     CDS             25157..26203
                     /locus_tag="JNX27_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7881387.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSTVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26331..29777
                     /gene="mfd"
                     /locus_tag="JNX27_000034"
     CDS             26331..29777
                     /gene="mfd"
                     /locus_tag="JNX27_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7881388.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     SERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            29920..30882
                     /gene="ldtC"
                     /locus_tag="JNX27_000035"
                     /pseudo
     CDS             29920..30882
                     /gene="ldtC"
                     /locus_tag="JNX27_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="JNX27_000036"
     CDS             complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="JNX27_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7881389.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31463..32095
                     /gene="comR"
                     /locus_tag="JNX27_000037"
     CDS             31463..32095
                     /gene="comR"
                     /locus_tag="JNX27_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7881390.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32157..32696)
                     /locus_tag="JNX27_000038"
     CDS             complement(32157..32696)
                     /locus_tag="JNX27_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7881391.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(32923..34227)
                     /locus_tag="JNX27_000039"
     CDS             complement(32923..34227)
                     /locus_tag="JNX27_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7881392.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34628..35170)
                     /locus_tag="JNX27_000040"
     CDS             complement(34628..35170)
                     /locus_tag="JNX27_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7881393.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="JNX27_000041"
     CDS             complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="JNX27_000041"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7881394.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="JNX27_000042"
     CDS             complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="JNX27_000042"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7881395.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="JNX27_000043"
     CDS             complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="JNX27_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7881396.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(37690..38067)
                     /locus_tag="JNX27_000044"
     CDS             complement(37690..38067)
                     /locus_tag="JNX27_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY7881397.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="JNX27_000045"
     CDS             complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="JNX27_000045"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7881398.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            38769..40958
                     /gene="fhuE"
                     /locus_tag="JNX27_000046"
                     /pseudo
     CDS             38769..40958
                     /gene="fhuE"
                     /locus_tag="JNX27_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41018..42451)
                     /gene="ptsG"
                     /locus_tag="JNX27_000047"
     CDS             complement(41018..42451)
                     /gene="ptsG"
                     /locus_tag="JNX27_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7881399.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42746..43543)
                     /locus_tag="JNX27_000048"
     CDS             complement(42746..43543)
                     /locus_tag="JNX27_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7881400.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43554..44558)
                     /gene="holB"
                     /locus_tag="JNX27_000049"
     CDS             complement(43554..44558)
                     /gene="holB"
                     /locus_tag="JNX27_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7881401.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44555..45196)
                     /locus_tag="JNX27_000050"
     CDS             complement(44555..45196)
                     /locus_tag="JNX27_000050"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7881402.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45186..46208)
                     /gene="yceG"
                     /locus_tag="JNX27_000051"
     CDS             complement(45186..46208)
                     /gene="yceG"
                     /locus_tag="JNX27_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7881403.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46211..47020)
                     /gene="pabC"
                     /locus_tag="JNX27_000052"
     CDS             complement(46211..47020)
                     /gene="pabC"
                     /locus_tag="JNX27_000052"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7881404.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47140..48381)
                     /gene="fabF"
                     /locus_tag="JNX27_000053"
     CDS             complement(47140..48381)
                     /gene="fabF"
                     /locus_tag="JNX27_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7881405.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFSCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48468..48704)
                     /gene="acpP"
                     /locus_tag="JNX27_000054"
     CDS             complement(48468..48704)
                     /gene="acpP"
                     /locus_tag="JNX27_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7881406.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(48915..49649)
                     /gene="fabG"
                     /locus_tag="JNX27_000055"
     CDS             complement(48915..49649)
                     /gene="fabG"
                     /locus_tag="JNX27_000055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7881407.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49662..50591)
                     /gene="fabD"
                     /locus_tag="JNX27_000056"
     CDS             complement(49662..50591)
                     /gene="fabD"
                     /locus_tag="JNX27_000056"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7881408.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50607..51560)
                     /locus_tag="JNX27_000057"
     CDS             complement(50607..51560)
                     /locus_tag="JNX27_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7881409.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLGRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51628..52698)
                     /gene="plsX"
                     /locus_tag="JNX27_000058"
     CDS             complement(51628..52698)
                     /gene="plsX"
                     /locus_tag="JNX27_000058"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7881410.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52779..52952)
                     /gene="rpmF"
                     /locus_tag="JNX27_000059"
     CDS             complement(52779..52952)
                     /gene="rpmF"
                     /locus_tag="JNX27_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7881411.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53004..53525)
                     /gene="yceD"
                     /locus_tag="JNX27_000060"
     CDS             complement(53004..53525)
                     /gene="yceD"
                     /locus_tag="JNX27_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7881412.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            53724..54308
                     /gene="yceF"
                     /locus_tag="JNX27_000061"
     CDS             53724..54308
                     /gene="yceF"
                     /locus_tag="JNX27_000061"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7881413.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54420..55379)
                     /gene="rluC"
                     /locus_tag="JNX27_000062"
     CDS             complement(54420..55379)
                     /gene="rluC"
                     /locus_tag="JNX27_000062"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7881414.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            55952..59137
                     /gene="rne"
                     /locus_tag="JNX27_000063"
     CDS             55952..59137
                     /gene="rne"
                     /locus_tag="JNX27_000063"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7881415.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59334..60287)
                     /gene="flgL"
                     /locus_tag="JNX27_000064"
     CDS             complement(59334..60287)
                     /gene="flgL"
                     /locus_tag="JNX27_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY7881416.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60299..61941)
                     /gene="flgK"
                     /locus_tag="JNX27_000065"
                     /pseudo
     CDS             complement(60299..61941)
                     /gene="flgK"
                     /locus_tag="JNX27_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62007..62948)
                     /gene="flgJ"
                     /locus_tag="JNX27_000066"
     CDS             complement(62007..62948)
                     /gene="flgJ"
                     /locus_tag="JNX27_000066"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY7881417.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(62948..64045)
                     /locus_tag="JNX27_000067"
     CDS             complement(62948..64045)
                     /locus_tag="JNX27_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7881418.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64057..64755)
                     /gene="flgH"
                     /locus_tag="JNX27_000068"
     CDS             complement(64057..64755)
                     /gene="flgH"
                     /locus_tag="JNX27_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7881419.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(64808..65590)
                     /gene="flgG"
                     /locus_tag="JNX27_000069"
     CDS             complement(64808..65590)
                     /gene="flgG"
                     /locus_tag="JNX27_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7881420.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65728..66479)
                     /locus_tag="JNX27_000070"
                     /pseudo
     CDS             complement(65728..66479)
                     /locus_tag="JNX27_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(66499..68427)
                     /gene="flgD"
                     /locus_tag="JNX27_000071"
                     /pseudo
     CDS             complement(66499..68427)
                     /gene="flgD"
                     /locus_tag="JNX27_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68439..68842)
                     /gene="flgC"
                     /locus_tag="JNX27_000072"
                     /pseudo
     CDS             complement(68439..68842)
                     /gene="flgC"
                     /locus_tag="JNX27_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(68846..69019)
                     /locus_tag="JNX27_000073"
     CDS             complement(68846..69019)
                     /locus_tag="JNX27_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAY7881421.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69174..69833
                     /gene="flgA"
                     /locus_tag="JNX27_000074"
     CDS             69174..69833
                     /gene="flgA"
                     /locus_tag="JNX27_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7881422.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            69909..70202
                     /gene="flgM"
                     /locus_tag="JNX27_000075"
     CDS             69909..70202
                     /gene="flgM"
                     /locus_tag="JNX27_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY7881423.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70207..70623
                     /gene="flgN"
                     /locus_tag="JNX27_000076"
     CDS             70207..70623
                     /gene="flgN"
                     /locus_tag="JNX27_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7881424.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINCSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70663..72198)
                     /gene="murJ"
                     /locus_tag="JNX27_000077"
     CDS             complement(70663..72198)
                     /gene="murJ"
                     /locus_tag="JNX27_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7881425.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72365..73231)
                     /locus_tag="JNX27_000078"
                     /pseudo
     CDS             complement(72365..73231)
                     /locus_tag="JNX27_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(73233..73880)
                     /locus_tag="JNX27_000079"
     CDS             complement(73233..73880)
                     /locus_tag="JNX27_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7881426.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYLLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(73891..74475)
                     /gene="rimJ"
                     /locus_tag="JNX27_000080"
     CDS             complement(73891..74475)
                     /gene="rimJ"
                     /locus_tag="JNX27_000080"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7881427.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HTCYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74711..75919
                     /gene="mdtH"
                     /locus_tag="JNX27_000081"
     CDS             74711..75919
                     /gene="mdtH"
                     /locus_tag="JNX27_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7881428.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            75983..76630
                     /gene="grxB"
                     /locus_tag="JNX27_000082"
     CDS             75983..76630
                     /gene="grxB"
                     /locus_tag="JNX27_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7881429.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76764..77324
                     /locus_tag="JNX27_000083"
     CDS             76764..77324
                     /locus_tag="JNX27_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7881430.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77430..78476
                     /gene="pyrC"
                     /locus_tag="JNX27_000084"
     CDS             77430..78476
                     /gene="pyrC"
                     /locus_tag="JNX27_000084"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7881431.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78550..78795
                     /gene="dinI"
                     /locus_tag="JNX27_000085"
     CDS             78550..78795
                     /gene="dinI"
                     /locus_tag="JNX27_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY7881432.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEIPQETWESADDWFVSE"
     gene            79085..79339
                     /gene="bssS"
                     /locus_tag="JNX27_000086"
     CDS             79085..79339
                     /gene="bssS"
                     /locus_tag="JNX27_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7881433.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79454..80572
                     /gene="solA"
                     /locus_tag="JNX27_000087"
     CDS             79454..80572
                     /gene="solA"
                     /locus_tag="JNX27_000087"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7881434.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80620..80733
                     /locus_tag="JNX27_000088"
     CDS             80620..80733
                     /locus_tag="JNX27_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7881435.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            80994..81560
                     /locus_tag="JNX27_000089"
     CDS             80994..81560
                     /locus_tag="JNX27_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7881436.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81564..82139
                     /locus_tag="JNX27_000090"
     CDS             81564..82139
                     /locus_tag="JNX27_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7881437.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82181..83233)
                     /locus_tag="JNX27_000091"
     CDS             complement(82181..83233)
                     /locus_tag="JNX27_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7881438.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83458..84378
                     /locus_tag="JNX27_000092"
     CDS             83458..84378
                     /locus_tag="JNX27_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7881439.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84550..84747
                     /locus_tag="JNX27_000093"
     CDS             84550..84747
                     /locus_tag="JNX27_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY7881440.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            84771..>85902
                     /locus_tag="JNX27_000094"
     CDS             84771..>85902
                     /locus_tag="JNX27_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7881441.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYWDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20618 a        22857 c        21159 g        21268 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttc gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgtgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgttgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatgg tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgcgtggtgt ggttctgata ttcctactct gctaatttgc tcagccccat
     6601 gtctgcgtaa gtgcgattta ctgcatttct caggtctgcg tagttctccg ttggcggctc
     6661 cggtgtgccc ttgctgctct gttttaccag cgggtcgccc accagccgcc cgtaagctgc
     6721 aatttgtgct gtcatattgc cacctcagtg aagcgatccg gcattgttct ttttcatctc
     6781 ttccatcatt ttttcagtga gcattacgca ctgaaacccc atgtttttga tgtctacggg
     6841 tttcatgcct ttgtcagtgg atatcacagg cagaatatcc tgatacacat cgcggtcagg
     6901 gtaactaagc acacaaagcg cccatgatgc cacgggtgag gatccttcgt ctgtttcaac
     6961 ccaccagcct ggtgtagctg cgcttatttg cagaatcgtg atccgtccgt tgtgcttgta
     7021 aaatttttca ccaaattttg gttcgctcat gattccacct ctccggtttt aacgttgatt
     7081 gttgttacct gttccgcttc ggcaatctcc cgctctgtca gcgtggcaaa atttgccgcc
     7141 gtcgtggtca tgaatgcgct tatcagttcg ggatgtgctt tcgcgtatcc ttcccccgcg
     7201 tggcggtcta tcgttctgat tgccaccttt aaagcgtgct ctgtcatgtc taacgcgcga
     7261 tatttcggtt ctgttctgtc tctgcgtttt ttgagtgatt tattaaattt ccctgaagtg
     7321 tgcatattta tttttacccc ctcgtttaaa aagttttgag ttgtgcctcc ccttgtctac
     7381 cttagtggcc ctcatgccag taatggcgct gctttcagcg gggtagagtg cttttatcca
     7441 ctatctaccc cgtgtctacc tccctgtctg attcaggtaa aatcaggtag agagggtaga
     7501 tagtgggtag acagtaaaaa aaggctacct acctaactta atgcactgaa ttaaatgtat
     7561 ttttctttac tcaggtagac agggtagaca gcaattacaa aaaattataa aaacgcgtcg
     7621 cactcgtctg ttgttattgc gttagtctgc gttactccct taactttccg cgtaatatat
     7681 tcatgtccgt aaactttcgc ggctggcttc atagccttgc caaagtcatt tacgtttagc
     7741 ggtttgctcc tgcctgcgta cgccataaac gccagataga cgcggtaaag gctgtttctg
     7801 gtcgtgtact tcactgaatc gccaccgccg cccatcatca ggccgcgtgc ttcctccaga
     7861 aaattcagga actggcaaaa ctcaataacc ggatccgtct gttgctttat tgccagagct
     7921 tcatcaccgt cacgctgttc cagtagtaaa gcccgtgctt tctcagagtc ggtaaagttc
     7981 gccagcaatc ggcggataat gacagggatt tcagccgcaa tcttttccgg tagctccctg
     8041 tctttttcgg cctcactaac gatattgtcg aaacggaaaa tcacgcgacg acgtgccaca
     8101 cctccggccc gttcggtgaa tatcatcggg ttattgttgg tcgccagcac caccgccctg
     8161 attacagccg tgaaacgctt ttcgtatttc gggttaattt ccacggggtc accgcccgtg
     8221 attttcttga tgcccgttcc ttcgcctgta tatttcggct ggtcagccag gacgataaga
     8281 cgactcccga caacctgcgg acgtccacca gcatcatcaa gcgatgtcat ttcagcgctt
     8341 accgtgttct gtttccctgc cagaaggctg gctatgtgtg tgaatgtact tttaccgctc
     8401 ccgccgtctc cggtggcctc aataaacatc tgccagtcgt accggttcgc cataatcatg
     8461 tacagcgcgg cacatatacg catcatcttg cgctggtctt ttccggctgc gtgctcaagc
     8521 catttatgaa agtttagcgc gttatcgcgg atgttctccc ctggtgctgg tggcgtgtac
     8581 tcaatgccgt tgtgcgtggt gatccagttc tccggcgtgt gcggggaaaa ttcccccgtt
     8641 ttcaggtcaa gcgcaccatt ggcgaacggc agcaaatcgc cggacggctc gcccattggt
     8701 tcggcaataa cttttaacgc ttccacggcg ttattgatta cgcgcttgct gaaagtggcc
     8761 ctgtgctctg aatagatcgc caccatttcg cggctaagtt ccattgtgct gaccggacac
     8821 cataccccgc cgcgccatac gtgaacgatt tcactttcct gatgcacaca aacgccatca
     8881 aagcgctcgg caagcagctg cgcgcgctca ctgtctgcca tttgtgccag ttgcgtattt
     8941 ttctgctccg gcatggtaag ccctgcggca atattttcac gctcagcatt cagatagcgc
     9001 cgccagtttt cacacttctg gacgtgcatt ccttcggggt aaaaatttgc gtcctgaata
     9061 tctgccgccg ccagcttctg accaatcttt ttggtctcca ccaaatccag ttctccggcc
     9121 tggtacagcc ttacgcgttt ttttccatcc ggaacaattt tcagtgcatc aagttcggca
     9181 agctgatttg gcccaagcca cacaggcggc acattatcgc cggatgcggg gccatcctgc
     9241 tcctgccatt gtttcgcgtg cgaccaggca tcactacccg caaagataat tacctctgtg
     9301 tctttgtgtt ttattccgcg tggctgtttt tttacgttcg gtgccagttt cattttttac
     9361 ccctgaatac gttaagcatc ttttttattt cctgaatatt ggcgcgtgct ttctccctgc
     9421 tggttggttt actgcggggc gctgcctgta ccagcgtgaa atcacgccgg aattgataaa
     9481 caggcatcac gcaatcatat tcgtaacctt cacggcggta ggtgatgcgc cgttctgcca
     9541 cgcctttaat cgttaccatg ccgccgtact ggtcgcggta aatatcaccg cgcgtaaatt
     9601 tagggtgagt gttgccactg gcagttaagc cagaatattt aagtttcatt atttttattc
     9661 tccggtgtgc tgttctttat atctgtcgtg caatagatct atttcttgca gttccattat
     9721 tacaggctca agaagcgtta ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc
     9781 attgtcgcca agtgtttcaa gccatatgcg caatatttcc agcatgtttt cactgtgaga
     9841 aagtgcaaga aatgcgcggt ctattgtttc gtggtaaata tcacgcatat taatccccgt
     9901 ccgtcgtttt tcttaaaata acctctgtca cgaaatcagc ataatcggcg gcgatattca
     9961 gtacatcaag cccgttgatt tatattctct cgtggaaagt aagaaaaaag ccgctctaat
    10021 aagttctggc attgacgaaa gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg
    10081 tttcagggaa ttaaaacgat catcacgcat aacccccccc attttcacaa tcggcaacaa
    10141 gaatattttt agcgtcattc agtgaccgcg ttgcgatgta tcggatacat tccagggcga
    10201 actgtgtccg gtctttctgt tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa
    10261 taagcgcgtg catcagcgta gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt
    10321 gcataggttt ccccctgacg aatacgggcg gcgaatacag caacacaacc ggacgggcaa
    10381 cggctacgcg cttcgcgttc cgtccaagcg gttacgtgga tgatttggga ttctccggta
    10441 ctcagtgcca gaaaacgcca cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga
    10501 tttacggcaa ccataacggc tcctagttta cgttgttggt tagaagcccc gttactgctc
    10561 ctacagtgcg gggctttgct ttttgtgttg attgtgtgta atgtgtcatt acacataaat
    10621 acattacatg aggtgtaatt aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc
    10681 cgttccagtt ccgtttagat cctgacttac gggaaaagat ggaaaaggca caacaacagg
    10741 acggcgatga ttctcttgct gcctggataa agcgaataat ccgcaaagaa ttacagcaac
    10801 gcggcataga acccaaagga taaaaactat cagcgccgtg gtgtgaggta ctacggcgca
    10861 ttgctttaca ggcgcacaca atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac
    10921 gaaaaagacg acgcagaaag aaagagcatg aatcagaacg attcgcgccg tgttcttttg
    10981 cccttgagaa gtaccttaaa gaatactcat caaaggagag aacttcgcag ttatggcaac
    11041 gcactgaacc agactgattg cattgcccac cagcctgata gcggctatca tccccgtgct
    11101 tatgtttggg atcacacaca caaacggcgc agcgggttat ctgttcagaa aggcggctcc
    11161 atttcggggc cgcttttttt atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac
    11221 cagggcgaac gaatccccgc ccacgttctg gcgtatattc aatcttcatg gttatagctc
    11281 tgtgttcaga tgattgatgt gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct
    11341 tgcagctatc cttccaggcc agaacctcgg ataaagacca gccaacggaa cgaccgccaa
    11401 gtttacgacg tgatgggaat tgtccggcct tttccaggcg gtagcggcat gagcggctaa
    11461 ggcctgttag cttttcgcat tctttttcac gtataaaccg atcagtgctt aacactattg
    11521 ccccctttcg tttcttaaag agttatttcg tgttctattg cgttgggatg tgtctgattg
    11581 tgtcaggatg attcagagtt ggcaaatgtt gaggccgtat ggtttacaga aagaggaaca
    11641 atcaggataa aatcatttaa attcatgtta atacaaaggc ataaaatatt gtttcatgcc
    11701 ttttttctcg ctatttaaag agtgattcgc tagtgtataa aaaaccagta acacgttaaa
    11761 aatcagctac ttataaatcc gtacactttt tcacctcttg ttcgtagttg ttccgcattg
    11821 ttgctcattg ttgcacgttg tatctgttcg catatccagt atgcgcatac tgaaaaaaca
    11881 cgaaaaaaat tattttcttc tggctactgg tagcgtggtt acgttttcat gtgttcccgc
    11941 cagtatccct aaccgctccg tccacatatc cagcgcattg cgtttagcat ccagataacg
    12001 ggaatgatta taaactcgct gcattcctgg catctggtgg cctgtaagct gctccacgac
    12061 gtgaggatcc acgcctaaat cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag
    12121 tgaccagtga gggtgattta gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt
    12181 ttcctgtttc acttccccca gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt
    12241 gacgaacggc agtattgctt ccggtatggg ccggaatatt gccaccttcg ttttgctgtg
    12301 ttctttcggc acggtccaga gcatttcggt aaaatcccac tcgctgatct ccgataacct
    12361 cagttctacc gtccggcatc cgaacacaat caggaggcgg attaacgcga tgtagtaggg
    12421 ggagaatatt tttttgtcca gtgcctgcaa taattcgccc agttctttgg tgcttaagac
    12481 acgctcgctt atatccggtt ttttcccaac gtccgccaca ctcatatcat caagaacgtt
    12541 gctgattgca tagcgccgcc tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa
    12601 gaatccggca gtaacaggcg ttcgctttgc cacctggtca aaacaggcca gccagtgccg
    12661 tagctcgcat ttatccagcg gcatagcacc aatgtgctgt attacgtgat tattaaggcg
    12721 ctttttcagg gcggcataat ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt
    12781 gagcgcatcg ccaaccgtta ccggctttaa cgcttcctgt acggtgtaat taagctcatg
    12841 acgtggattt ttcccctctg ccagccatgc gcgacactgg gcggcttttt ccctggctga
    12901 tttcaggctc agatcaggat aatttcccag cttaatgcgt tccggtggtg ccccccttcc
    12961 cgttccggcc ctgtaagtga aataccaggt taaaaggcca ctggttgaat gcctgacgct
    13021 caggtttcca ccgtcattaa gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct
    13081 cagctgtgta tcgctcagtt tgttaagtgc tctgctcata attttgattc cggtcatcac
    13141 aattaactac accattaact acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc
    13201 tggaacaagg aaaatcacaa atctgtttat aatcaaaaac ataatgaaca atctgaaaca
    13261 ttatgaaacg gcaaaaaaca ctaattgata aaatatgtcc atacttcacg cattacgtta
    13321 agcatccgtt ataatcggtt gcagatacca gcctgtggat gcttaacatg gaataccaac
    13381 tcactcttaa ctggcccgat tttcttgaac gtcactggca gaaacgcccg gtggtgttaa
    13441 aacgcggctt taataatttt attgacccga tctctccaga cgagttggca ggtctggcga
    13501 tggaaagcga agttgacagt cgactggtca gtcaccagga tggcaaatgg caggtcagcc
    13561 acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg
    13621 cagtaaacca ctggcatgag ccgaccgccg cgctgatgcg accgttccgt gaactaccgg
    13681 actggcgtat tgatgatctg atgatttctt tttctgtacc tggcggcggc gtcggcccgc
    13741 atctcgatca gtacgacgtg tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg
    13801 gcgaaaagct gcaaatgaaa cagcactgcc cacatccaga tctgttacag gtcgatccgt
    13861 tcgaagccat catcgatgaa gagctggagc ctggtgatat tctttatatt ccgccaggat
    13921 tcccgcatga aggctacgcg ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc
    13981 caaatacgcg ggaattaatt agcggatttg ccgattatgt gctgcaacgt gaactgggcg
    14041 gcaactacta cagcgatccg gatgttccac ctcgcgctca tcctgcggat gttctgccgc
    14101 aagagatgga taaactgcgt gagatgatgc tcgaattgat caaccagccg gaacacttta
    14161 agcaatggtt tggcgagttt atatcccagt cacgtcatga actggatatc gcgccgccgg
    14221 aaccgcctta tcagccagat gaaatctacg atgcactgaa acaaggtgat gtgctggtgc
    14281 gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga
    14341 tcgattcccc gcaccgtccg gcactggatg cactcgccag caacattgcg ctgactgcgg
    14401 agaattttgg cgatgcgctg gaagatccgt cattcctcgc gatgctcgcg gcgctggtca
    14461 atagcgggta ttggttcttc gaagggtaag tttggattta agccggatgc agcatcacat
    14521 gccgcatccc ttttcgctta cttccgttgc gccgttaact cggcaatacg gacgatcacc
    14581 tgcaccgctt tttccatacc ttccagaatc acaaactcat gcttaccatg atagttgtaa
    14641 ccgccagtga acaggttcgg gcaaggtaat cccataaacg acaactgcgc gccgttggta
    14701 ccgccgcgga tcggtttcag ttccggttca atatcgcagt cacgcatcgc ctgctgggcg
    14761 atatcgagaa tatgcggatg ctcaaccact ttctcgcgca tattgtagta actgtcttca
    14821 atcaccaatt caatgtagca atcaggatgt aaccctttgc ccactttttt ggcgatctcc
    14881 atcattttac gtttacgcgc ttcaaactgt ttacggtcga aatcacggat gatgtagtgc
    14941 atatcggccc gttcaacggt gcctttcatg ctcgccaggt gatagaaacc ttcatagcct
    15001 tctgtcattt ccgggctttc atccgccgga acttccgcat gaatacgtgc cgccagcgac
    15061 agcgcattta ccatcactcc tttcgccgtg cccggatgaa cattgttacc gacaattttg
    15121 atattgaccg atgcggcgtt gaagttttca aactccagtt cgcctacgcc accaccgtca
    15181 acagtgtaag cccagcgggc atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc
    15241 acttcttcat ccggggtaaa ggcgacgcga atatcaccat gcggaatgtt tttctgttgc
    15301 aataccgcca gcgcggtcat gatttctgca atacctgctt tgtcatcggc acctaacaag
    15361 gttttaccat cggtggtaat caacgtctga cccagtagct gatgcagcac cgggaacata
    15421 accggtgata aaacttcatc gccgataccc agcgcaatat cgccaccgcg atagttttca
    15481 acaatttgcg gattaacatt tttgccgctg caatccggtg aggtatccac atgagaaata
    15541 aagccaatcg ccgggatatc gccagggacg ttagccggta acgtcgccat caaagtgccc
    15601 ttctcactta aggtcacatt gataagcccc atttcttcga gctgctcttt cagcagatgc
    15661 aataacttcc attggccttc cgtgctggga acctgtctca cccctgcttt tgattgggta
    15721 tccagagaca cgtagttcaa aaatcgctca agtagtttat ccatgtagtc accctcactt
    15781 tttgtgacaa cattattaag aagcaagaaa agacaaatat tgcgtcaggt cacttttacc
    15841 cctgcaagcg ggaatattta tcagcattac cttaatgaat ataaagctaa gccagtaatt
    15901 cacaacaaag attggcgatt caagcggttt gccgtagaat taccgccctc attaagagtc
    15961 accaaggtgg ttaaccacaa accccgcatc ggtaagccat ccgttgcgtt tacatgggac
    16021 agagtaaaaa attgaataaa caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa
    16081 ttcgcaaatg ctttgatggt aaagaggtca ttccccagct ggatctgact atcaacaatg
    16141 gcgagttcct cacgctgctt ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga
    16201 ttgcaggtct ggaaactgtt gattccggac gcatcatgct ggataacgag gacatcaccc
    16261 acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca aagctatgca cttttccccc
    16321 acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg
    16381 aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca gctggaaacc ttcgctcaac
    16441 gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg
    16501 ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc agcgctggat tacaaactgc
    16561 gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt
    16621 tcgtgactca cgaccaggaa gaagcactca ccatgtcgga caggattgtg gtgatgcgcg
    16681 atggtcgcat tgagcaagac ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt
    16741 ttgttgccgg gttcattggc gaaattaata tgtttaacgc cacggtgatc gagcgactcg
    16801 acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg taatatctac gttaacttcg
    16861 ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag
    16921 agattaacga cgacaaccac gctgaagggc tgattggtta cgttcgtgag cgtaactaca
    16981 aaggcatgac gctggagtcg gttgttgaac tggaaaatgg caagatggtg atggtcagcg
    17041 aattcttcaa tgaagacgat cctgactttg accactctct cgaccaaaaa atggccatta
    17101 attgggtaga aagctgggag gtcgtactgg ctgatgaaga acacaagtaa gttccagaat
    17161 gtagtgattg tcactattgt cggttggctt gtgttgtttg tctttctgcc caacctgatg
    17221 atcattggca ccagcttttt gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg
    17281 ctggataact acacgcgtct gctcgatccg ctctattttg aagtgctatt gcactcgctg
    17341 aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg gctatccatt tgcctggttt
    17401 ctggcgaagc tgccacacaa ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc
    17461 tggaccaact cattgattcg tatctacggg ctgaaaattt tcctcagcac caaaggctat
    17521 ctcaacgagt ttttgctctg gctgggcgtt atcgacacac caatccgtat catgttcacg
    17581 cccagtgcgg tgattatcgg tctggtttac attctgctgc cgtttatggt gatgccgctg
    17641 tactccagta tcgaaaaact ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc
    17701 agcaagttac agacttttat ccgcatcatt attccactga cgatgccggg gattattgcc
    17761 ggatgtctgc tggtgatgct gccagcgatg ggcctgttct atgtgtccga cctgatgggc
    17821 ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtac agttcctcaa tattcgtgac
    17881 tggccgtttg gtgcagccac cagcattacg ctgactatcg taatgggcct gatgttgctg
    17941 gtttactggc gcgcttctcg tttgctgaat aagaaggtgg aactcgaatg atcggtcgac
    18001 tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca atcattattt
    18061 tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt tttaccacca
    18121 aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag cattcactaa
    18181 caatggcggt gttttcggcg acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc
    18241 tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga
    18301 tgtcaccaga tatcgtgatg gcaatttcgc tgctggtgct gtttatgctg ctgggtattc
    18361 agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg
    18421 tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg gcgaaagatc
    18481 tcggtgccag cgaatttacc attctgcgaa aaatcattct gccactggca atgccagcgg
    18541 tggcggcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta gtttcttcat
    18601 tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg
    18661 gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg
    18721 ttattgccag ccagcttatt gctcgtgata aaacgaaagg taacacaggg gacgttaaat
    18781 gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca tgagcgccgc
    18841 tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg
    18901 actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc
    18961 gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcctatg acctggtggt
    19021 tccttcaacc tattacgtcg ataaaatgcg taaagagggg atgatccaga agatcgacaa
    19081 gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc cttttgaccc
    19141 gaataacgac tactccattc cgtatatctg gggtgcgacg gcgattggtg ttaacggtga
    19201 tgcagtggat ccaaaatctg tcaccagctg ggccgatctg tggaagccag agtacaaagg
    19261 cagccagctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc gtaagctggg
    19321 ctactccggt aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa
    19381 actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg
    19441 cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac
    19501 gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat
    19561 cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc
    19621 ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg
    19681 taagctgtta agtccagaag tggcaaacga taagacgctc tacccggatg ctgaaaccat
    19741 taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta
    19801 tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt
    19861 ttagttagca aggatgtggc taatgagtca ggacagtaaa gttttatccc gctctttcgt
    19921 cggtattgtg gtccgcttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct
    19981 ttataatccc tccagcaact tttcaacaaa ttctggcacc acctggcttg ccgggccgta
    20041 atatttctcg gcaaattcat taccaacctg actcggttca agattcagtt caacggtgtg
    20101 agcaccatgt agtttggctt cgtgtacaaa cccagcagcc ggatagacat gcccggaagt
    20161 accaatggca ataaagacat ccgccatcga caacgccata taaatttcat ccatgccgag
    20221 tggcatttcg ccaaaccaaa cgacgtgcgg acgcaagggt gccgggaact ggcaacaatg
    20281 gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca
    20341 acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg
    20401 caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt
    20461 agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg
    20521 ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac
    20581 atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga
    20641 ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg
    20701 caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt
    20761 tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta
    20821 atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg
    20881 ttcaatgcgc ggaacacgag ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg
    20941 cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac
    21001 aatggtcagg atatttccca gagaaaccgc taataaatcc agataacgct caacgtgcgc
    21061 ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct gcaacggttg
    21121 atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca
    21181 gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca tggttaacgc
    21241 atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac cagtaatcgg
    21301 tttgccgttg aaaaccagcc cgccgccaac gccggtgccg agaattaacc ccatcaccag
    21361 tggatattgc gtaaattcat cctcccaggc ttctgaaagg gcaaaacagt tggcatcgtt
    21421 atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct
    21481 ggcagcgggg acattagcgg catacagctt accatcttct gtttccggca ttcccggaat
    21541 accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta ccagctcaca
    21601 cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg
    21661 caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc
    21721 gtaatacatt gcctctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg
    21781 cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca
    21841 atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa
    21901 taaaatagat atcgctggag aggaactgat gaccgatcag cttttcaatc cactcaataa
    21961 tcggggtaag ttgcagtgaa acaactacgc cgataaccac cccacacagg ctgccgaaca
    22021 gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca
    22081 gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga
    22141 cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga
    22201 tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta atataaacat
    22261 agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca
    22321 ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca
    22381 gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt
    22441 gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg gggatcatga
    22501 tcgacaccca atcgccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa
    22561 tttgctgttc gcccgcttta aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg
    22621 cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac ctggattgcg cgcagattcg
    22681 ctccactttc caccagaccg gtgaaattga tatacggcgc agcggcggca atacccggca
    22741 ctttctgcac gttatccagt gcttcctgcc agttagtcca tggctgattt accgcttcga
    22801 tttcgccatg cggcaccacc gccagaatgc ggttattcag ttcgcgttca aagccgttca
    22861 tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg gtagaaatga
    22921 cagagattag cgacaccatg ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca
    22981 ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg
    23041 tccatcacgc atttccagtt ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac
    23101 caccaggaaa gcggtgccct gcaagcgatt caactctcca agcaactgga agatgctgtc
    23161 ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccagac gcggattatt
    23221 gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg
    23281 gtgattcgca cgatgctcca gccccaccgc ttttaacatc tccagtgcac ggctgttgat
    23341 ttcagcgggc tttttcttgc caatcagcag tggcatagcc acgttttcca gggcagtaaa
    23401 atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagttctgc
    23461 tttcgccgct gaagacagtt tgctcatcgg ctgaccgtta aagatcacat cgccggaggt
    23521 tggcgtatcc agcccgccta acaggtgcag caaggtactt ttaccggaac cagagctacc
    23581 gacaattgcc atcatttcac cttcgcccac gctgaaactg acattgtgca gcacatcggt
    23641 ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt
    23701 cataacgtaa agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag
    23761 acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag
    23821 ccaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact
    23881 ggctggcaag cagtgcgcca agcgccgctc cgaggattgc accgataatc ccggcgctgg
    23941 ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga
    24001 tcgcgacttc gccctgcttc tccatcacca tcagccccag cgaggtaata atgttaaacg
    24061 ccgcaacagc gacaatcagg ctcagcagta agcccatcat atttttttcc atgcgtacgg
    24121 cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt
    24181 tttgctgact taaagagtca actttcagcg gctcatccag ccacaaacgc cagccggtaa
    24241 tattggctgc cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat
    24301 agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg cgctgacttg
    24361 gaatacgccc catcggcgtg aactggctgg cagatggcac catcacgcgg atttgatcac
    24421 cgcgattgac gcccaactgt gaggcaagtt gttcgccgag gatgacatta tatttccccg
    24481 gctcgaggtc agtttgtttc acattgacca gatacggcgt tagtggatct ttttgcgccg
    24541 ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac
    24601 cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt
    24661 gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga
    24721 tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca
    24781 tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg
    24841 ctgcacgccc acgcatgtaa cgcaagccaa taaatagagc gacaggttgg tacatgaaat
    24901 ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc
    24961 tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat
    25021 tgccatgttt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag
    25081 acagataccc attacacctc gggtcataac cagcaggatc aaccgcatgt aaattaaaaa
    25141 agagcgtgtt gttctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg
    25201 tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcata tgaccacttt
    25261 tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc
    25321 cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga
    25381 cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc
    25441 gctttggggc gtggtgcagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    25501 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    25561 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    25621 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    25681 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    25741 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    25801 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    25861 gctggtatcg actgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    25921 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    25981 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    26041 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    26101 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    26161 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    26221 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    26281 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    26341 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    26401 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    26461 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    26521 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    26581 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    26641 gcgtactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    26701 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    26761 aactggacag cgccggttat cgctatgttg accaggtgat ggagcacggc gaatacgcca
    26821 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    26881 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    26941 aggaagtaga agcgatcaat ctgctgcccg cacacgaatt tccgaccgat aaagcggcaa
    27001 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    27061 tttaccagca agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt
    27121 tcttcagcga accactgccg ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    27181 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    27241 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    27301 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    27361 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    27421 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    27481 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    27541 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    27601 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggtgcgtaat ctggcgctga
    27661 tctgcgaaag cgatctgctc agtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    27721 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    27781 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    27841 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    27901 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    27961 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    28021 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    28081 aacacgatcg tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg
    28141 atcaggcgca ggccattaat gcggtactta acgacatgtg tcagccgctg gcaatggatc
    28201 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    28261 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    28321 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    28381 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    28441 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    28501 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    28561 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    28621 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    28681 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    28741 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    28801 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    28861 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    28921 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    28981 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    29041 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    29101 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    29161 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    29221 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    29281 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    29341 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    29401 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    29461 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    29521 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    29581 gcgggatgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    29641 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    29701 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    29761 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    29821 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    29881 tgtttattac aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt
    29941 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    30001 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    30061 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    30121 ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    30181 ttaacgatcc cgttgccaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    30241 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    30301 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    30361 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    30421 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    30481 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    30541 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    30601 ctctttagct aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    30661 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    30721 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    30781 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    30841 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    30901 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    30961 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    31021 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    31081 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    31141 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    31201 tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa
    31261 ttacttaacg atcgataact aaatgataga tgtgatatgg atcacataca agatattttt
    31321 tataacaatc attaattaat ttaaaaatct ttaaatttca gtcaaataga aagcaattat
    31381 ttttatggga tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac
    31441 agtcaccagg tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg
    31501 tggccgccca aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg
    31561 gcaacacggt tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc
    31621 gcccacgcta tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg
    31681 ctatatcgat cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt
    31741 ggagtctgcg ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac
    31801 cccggcaggc tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat
    31861 cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg
    31921 tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt
    31981 ccttaattgt attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa
    32041 actgatgcag attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc
    32101 aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat
    32161 actttgttat tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt acccggatcg
    32221 cggtccatgc ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc
    32281 attttttctg acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg
    32341 ctttcctgga gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca
    32401 acagtggcga catctttacc gcgaccacca ccaaactgat gccctatcac gccaccagca
    32461 acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc
    32521 actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca
    32581 gaaacaacct gagcgtattg cgggccccgt tcaaacacgt tcagactggc cactgccgct
    32641 acgcccaacg cagctgcgac accaatcccg atacccgcca acattgattt attcacggga
    32701 acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga
    32761 cagaagatga aaagcgcggg aatgactgga aatacttaag gattcggagg agtctgaagg
    32821 aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag
    32881 ggcggataag gcgtttatgc cacatcagcc agtgtacgtc gattaatgca acttcaaacg
    32941 cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc
    33001 atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc
    33061 ttcaatcatc attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt
    33121 cgacagcgat accagcgaac catgatcttt atactgataa tttttcagcg gcttaccgtt
    33181 catctgcgcc agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg
    33241 cggcggaaca aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat
    33301 gtctggatcg cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt
    33361 ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccataccat
    33421 cagatcagcc tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt
    33481 gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc
    33541 agagatacgt ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt
    33601 cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga tcgcgttgtg
    33661 caattcagcg gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt
    33721 gccatttgcg cccaggttgg cagagtattt caggaacaga ttcagcatct cctggtggaa
    33781 gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt
    33841 gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga ttttacgttc
    33901 cggaaccaac agttcacctt tctcgtcgcg tagttctgca atagtgattg ttttcgcttc
    33961 acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc gcgcatgggc
    34021 cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag
    34081 tggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttacg
    34141 tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat
    34201 cacaatcttt ttcaatggcg tagtcaacgt gaccccctta aatttattaa ccaattgtta
    34261 attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt
    34321 cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt
    34381 ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagcg tttattattt
    34441 gagcaaattc ggaagagtaa tatagggtga agcgtatatc gagggcgttt ggtcgtcctg
    34501 ataagatgcg cagcatcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc
    34561 cttatccgac ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg
    34621 agggcattta cccgagggtt ttgaacgctt taatgcgctg taaatgcggg gagatattct
    34681 tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc aactcttcag
    34741 atgtccgctg gctgttaagt gcttcatcat tacgcgacaa aataaccaga caacgatcgc
    34801 gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc
    34861 gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc
    34921 ggatatcgca aagaaaacca atccgttccg cccagtatcc gcccaggcca acgccgcaaa
    34981 ttagcggacg ctcgtcaacg ttcagttgca acattttgtc cacttcttta agcagatgct
    35041 gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa
    35101 actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat
    35161 agataatcat cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag
    35221 cgttcatgta actgattcag acgggtgctg atcgctttcc agcgagccga gtccatcagt
    35281 tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt
    35341 gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg
    35401 caacccgcat ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggcacct
    35461 tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac ccagttcctg acgcaaaacg
    35521 gttttcagcc agtagggaga accgctcgcc ggacgcggat caacatcact gtagatcaca
    35581 tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata
    35641 tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct
    35701 gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc
    35761 ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc atgataagaa
    35821 cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa gctgatatca
    35881 atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc cagtttgccg
    35941 ccctcttcca ttcccaacag cgcagcaaag gattgtgctg ccggtaagcg ggtaaaacct
    36001 tcgcgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag atgattgcgc
    36061 gatgctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc
    36121 gtaaagagaa tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc
    36181 agttcgtaac cttcgacatc caacattact ggacccacac tgctctcctt attgttttat
    36241 taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg
    36301 ccagcggtac tcaaaccacc ctcctttgag catcagcagc cagggaaacc atcgcctgac
    36361 ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg
    36421 ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc
    36481 tccagcatac tcccagtcga tgagttttaa ccctgatgcg ctatgcacta aatttccggc
    36541 gtggacatcc atatgcaatg gacttaagcg taaaggtcgt ggttcccgcg ctttgcgcag
    36601 acgttttaac attcgcagcc aacccacagt ccgccgcgcc ggatcgcttt gctgccagta
    36661 cagttccagt aagggcaaca gcgttattcg ccagccaaaa cgtggttgtt gatgtagata
    36721 atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg cgccgggcag
    36781 atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa tgcttgcggg
    36841 tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat caggatcgtg
    36901 cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactac cgccactaag
    36961 cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc
    37021 gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg
    37081 tctgcaccag catcagctgc atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg
    37141 agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc
    37201 ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagt tgctgggcgg
    37261 aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg
    37321 cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg
    37381 cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt
    37441 cataatggcg aatatgcggc gcaggcggtg ccgttcgatc ttcgtgctca attgggcctg
    37501 gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt ggctcggctg
    37561 gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc cccacacacc
    37621 cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt gtcatcaaga
    37681 ttcacccctt tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
    37741 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
    37801 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
    37861 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
    37921 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
    37981 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acaccagaga
    38041 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
    38101 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
    38161 ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
    38221 gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgacac gtcgttcaca
    38281 gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
    38341 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
    38401 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
    38461 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
    38521 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
    38581 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgac
    38641 aaatatgaat gcgtatattt ctcatttgca tttgcatttg caagcaaaat tattcgcaca
    38701 ctaaatataa ccgctcatca ccctcctccg gcatgagcct gacgacaaca taaaccaaga
    38761 gatttcaaat gctttcaaca caatttaaca gggataatca atatcaagcc atcaccaaac
    38821 cgtcactact tgccggttgc atagcactgg cactattacc ttctgccgct tttgctgcac
    38881 cagccactga agaaacggtg attgttgagg gttcagccac agctccagat gatggcgaaa
    38941 atgattacag cgtaacgtct acctctgcgg gtaccaaaat gcagatgact caacgtgata
    39001 ttcctcagtc ggtcactatt gttagccagc agcggatgga agatcagcag ttacaaacgc
    39061 tgggcgaagt gatggaaaac acgctgggga tcagcaaaag tcaggcggat tccgatcgtg
    39121 ctctttatta ttcccgcgga ttccagatcg ataactatat ggttgatggt atccccacct
    39181 attttgaatc gcgctggaat ctgggcgacg cactttctga tatggcactg tttgaacgcg
    39241 tagaagtagt gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa
    39301 ttaatatggt tcgaaaacac gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat
    39361 acggtagctg gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg
    39421 gtaaaatccg cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct
    39481 acaatagtga aaagaccttc ttctcgggca ttgtcgatgc tgatttaggc gatcttacga
    39541 cgctttcagc cggttacgaa tatcagcgca ttgatgttaa tagcccaacc tagggcggtt
    39601 taccgcgctg gaatactgat ggcagcagca acagttacga tcgcgcacac agtaccgcac
    39661 ctgactgggc gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt
    39721 ttgctgatac ctggcaagcg acactgaatg ccacccactc tgaagtcgaa tttgacagca
    39781 aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca
    39841 gtaattatgg acctggcttt gatcatgtcg gcggcaccgg ttggaacagt ggcaaacgta
    39901 aagttgatgc gctggatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca
    39961 atctaatgtt tggtggcagt tacagcaaac aaaacaatcg ttacttcagt tcatgggcca
    40021 acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaaaccg
    40081 actggtcacc acagagcctg gcgcaggacg ataccacaca tatgaaatcg ttatatgctg
    40141 ccactcgtgt cacccttgcc gatccgctgc atctgatcct cggcgcacgt tataccaact
    40201 ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc
    40261 tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct attttccagc
    40321 cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg
    40381 agctgggtct gaaatcggac tggatgaata gccgtctgac caccacgtta gccatcttcc
    40441 gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg
    40501 aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg
    40561 gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatctt gcagaggata
    40621 acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg ttcaccagct
    40681 atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgttaactgg caaaatcgcg
    40741 tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc
    40801 tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca
    40861 ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc tacggcgcac
    40921 cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag
    40981 ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg atgtactcat
    41041 ccatctcggt tttcaggtta tcggatttag taccgaaaat cgcctgaaca ccagaaccag
    41101 caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat
    41161 cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac
    41221 caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct tttgcatctt
    41281 cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga
    41341 tggtgtagta aacaatcgca taaccgatac cgacgatcgg gaacagccac agtttgctgc
    41401 tgttaccaga cagaacgatg aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca
    41461 tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca
    41521 ggatcggcgc aacgaacatg aagaagaact cgatcggctc ggtgataccg gtcaggaacg
    41581 aggtcagcgc cgcggagatc ataataccgc ccactttcgc gcggttttct ggtttagcag
    41641 agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag
    41701 acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct
    41761 gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt
    41821 gcagaccaaa cggtaccaga caacgttcga tgaaaccgta aatgccaaac gcaactaccg
    41881 ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa
    41941 tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc
    42001 gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca
    42061 tgtacgctgc aatcgcaccg gagataatcc ctccgagtac gccagtatcc gccaagtgtt
    42121 tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt
    42181 taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa
    42241 agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg
    42301 cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc
    42361 ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat ttaccgacct
    42421 tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag tataggtgct
    42481 ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag
    42541 cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca
    42601 cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat
    42661 ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt
    42721 aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt
    42781 gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt
    42841 tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc
    42901 gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga
    42961 cataacgcgc agcatcgcac agttgctccg cattacggaa ggtcacaatg ccggaaaagg
    43021 agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt
    43081 gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac
    43141 gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat
    43201 ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg
    43261 tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt
    43321 agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc
    43381 ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt
    43441 tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat
    43501 aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga
    43561 tgaggaaccg gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg
    43621 gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg aatgtggcaa
    43681 acatccccca gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc
    43741 aggcccggca catcaacatt ggtcacctgc gcagcgccat gatggcgttt tagcgcatcc
    43801 atcagcaacg ttgccaccca gtgtaaacgc gccggagctt gttcatgatt aagggccgcc
    43861 agcagcgaat accagtcgcc cgatggcacg ctatatgcca acgcctgaca caatgtttca
    43921 cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgccaggcga accggcgctt
    43981 aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaagtc
    44041 acggcgtact gttctggagg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt
    44101 gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct
    44161 tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc atcggttacc
    44221 cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt gacctcacgt
    44281 accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta gtaatcggga
    44341 tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc
    44401 ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc
    44461 ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt
    44521 ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc ttcacccagt
    44581 gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg
    44641 tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga
    44701 aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc
    44761 caacttccgg ggtaacatcg agatagagcg ttaagtcggg gcgaaaatcc ccgagaacag
    44821 catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct
    44881 gagtggagag atcgtggcga tcgccaatca cccaggtgcc gttagccagc gcaggtttga
    44941 tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg
    45001 taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa
    45061 gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcgagcg
    45121 tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga
    45181 tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta
    45241 tgactggcaa gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga
    45301 tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc
    45361 gcacccggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct
    45421 gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc
    45481 tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca
    45541 ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg atttttatct
    45601 ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc
    45661 ttgtgagctc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat caaggtgtct
    45721 ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg
    45781 gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg
    45841 tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt
    45901 tccaacaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct
    45961 ttaaagtgag aaagatccgg ttcgatacgc agcagccatt gaaaaacccg tgggcgattg
    46021 atgatcttat cagcataaag ctgttcaccg agcgccagac gtccggtccc tggcttcagg
    46081 gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag
    46141 acgcccacac cagcggcgat acccagtacc accagcaata acaagataat caataacact
    46201 tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg
    46261 acgaaaaggt gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa
    46321 taaccatctc atctgcctgc aacgcctcct ccagcgaggc ttgcacttcg acaagctgat
    46381 aagaggattg tgccagtaaa cggatacaga attgtcgcat aatgccgtta acacctgcct
    46441 gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta gccgcacagc
    46501 attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa
    46561 gatgagagcg aatcaatact tgctcaagcc gattaagatg tttaatacct gcaagatgag
    46621 gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt
    46681 cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgctggtccg ctgttcaatg
    46741 tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca ccattttgct
    46801 gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca caggaaatca
    46861 tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc cgataacaaa ctgactttac
    46921 cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta
    46981 ctgccagcga ttcctgctta taaccgttaa ttaagaacat accggctcct tatcagagaa
    47041 ttgttagtgt ggcagcatgt tcactacgga acaagtcgga ataaaagcta agaaaaaagg
    47101 cccgcaagcg gaccttttat atgggtggaa aatgacaact tagatctttt taaagatcaa
    47161 agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac
    47221 ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccgggt tatccaggtt
    47281 gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag attctactgc
    47341 acccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca acacacggct
    47401 tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt
    47461 agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag
    47521 agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata
    47581 agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt
    47641 cgcgtgttcg tactcttcaa gtaccagcat accggcacca tcgcccagta cgaagccatc
    47701 acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc
    47761 acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg cgccacctgc
    47821 aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatggccaa tgttgtgcac
    47881 gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt
    47941 cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg
    48001 tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc
    48061 ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc
    48121 ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg
    48181 ttctttgcgc gagataatgt cctcacagct aaaatccttt actaagccag caaatttcgt
    48241 tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag
    48301 agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac
    48361 aactacacga cgcttagaca cgtttgtcct ccaaggaggg aaaaatgatt ctagtgggac
    48421 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
    48481 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
    48541 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
    48601 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
    48661 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
    48721 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
    48781 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
    48841 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
    48901 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    48961 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    49021 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    49081 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    49141 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    49201 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    49261 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    49321 ttagtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    49381 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    49441 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttggca
    49501 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    49561 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    49621 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    49681 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    49741 aggccagtaa gcaccttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    49801 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    49861 cggatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac agaaactgtt
    49921 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    49981 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gccagcagct
    50041 ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc
    50101 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    50161 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttccggtacg
    50221 gcttcttgca tgaacttacc gcgcatctca accaaacgca ccgcatcagc gaaatcaatc
    50281 acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    50341 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    50401 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    50461 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    50521 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    50581 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaagtgaat
    50641 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    50701 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc
    50761 accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    50821 ttagcctgat gcggaaccag ccagtccagt tgagaacggc caagattatt cgccgccagc
    50881 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    50941 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    51001 tcaccataac taccgtcggc atgcagatgg gtggaaatga ttcccggctc ttcagaggca
    51061 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    51121 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    51181 ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    51241 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    51301 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    51361 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    51421 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    51481 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    51541 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    51601 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    51661 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    51721 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    51781 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    51841 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    51901 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    51961 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    52021 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    52081 gattgtttta agcaccgctg aggcatcccg aatactgtca agacccttta cttcttcttc
    52141 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    52201 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    52261 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    52321 ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    52381 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    52441 tgaactccca cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    52501 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    52561 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    52621 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    52681 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    52741 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    52801 ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg
    52861 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    52921 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    52981 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    53041 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    53101 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    53161 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    53221 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    53281 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    53341 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    53401 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    53461 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcagggggaa ttttaccttt
    53521 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    53581 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    53641 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    53701 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    53761 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacct gaggtcgacg
    53821 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    53881 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    53941 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    54001 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    54061 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctaagcgagg
    54121 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    54181 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    54241 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    54301 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    54361 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    54421 tagcgcgcgt tacgcagttt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    54481 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    54541 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    54601 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    54661 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    54721 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    54781 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc
    54841 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    54901 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    54961 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    55021 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    55081 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    55141 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    55201 agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata
    55261 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt
    55321 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    55381 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    55441 gttttgcacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    55501 cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta
    55561 taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga
    55621 aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg
    55681 tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat
    55741 atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa
    55801 aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg
    55861 cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag
    55921 tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca
    55981 gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga
    56041 aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga
    56101 accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact
    56161 aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat
    56221 taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg
    56281 caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat
    56341 gccaaataac ccgcgcgcag gtggcatttc tcgccgtatc gaaggcgatg accgtaccga
    56401 attaaaagaa gcactggcaa gccttgaact gccggaaggc atggggctta tcgtgcgcac
    56461 cgctggcgta ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gcctgaaaca
    56521 ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga
    56581 gagcaacgta atcgttcgcg ccttccgcga ttatttacgt caggatatcg gcgaaatcct
    56641 tatcgataac ccgaaagtcc tcgaactggc acgtcagcat atcgctgcat taggtcgccc
    56701 ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca
    56761 gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccat ccggtggttc
    56821 cattgttatc gacagcaccg aagcgttaac agccatcgac atcaactccg cacgcgcgac
    56881 ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagccg ccgatgagat
    56941 tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat
    57001 gacgccagta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga
    57061 ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgcca
    57121 gcgcctgagc ccatcactgg gtgaatccag ccatcacgtc tgcccgcgct gctccggtac
    57181 cggtaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtttga tcgaagaaga
    57241 agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta
    57301 tctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa acgcgtcagg acggtgttcg
    57361 ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac taccacgtgt tgcgcgtgcg
    57421 caaaggggaa gaaacgccga ccttaagcta catgctgccg aagctgcatg aagaagcgat
    57481 ggcgctgccg tctgaagaag agttcgctga acgtaagcgt cctgaacaac ctgcactggc
    57541 aacctttgcc atgccggatg tgccgccagc gccaacccca gctgaacctg ccgcgactgt
    57601 cgtagcccca gcaccgaaag ctgcaacggc aacaccagca gctcctgcac aacctgggct
    57661 gttaagccgc ttcttcggcg cactgaaagc gctgttcagc ggtggtgaag aagccaaacc
    57721 gaccgagcaa ccaacaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa
    57781 acctcgtcag agcaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac
    57841 tgaaggcaac gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcaaac
    57901 tgccgagacg cgtgagagcc gtcagcaggc tgaggtaacg gaaaaagcac gtaccaccga
    57961 cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc
    58021 gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga
    58081 agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg
    58141 ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc
    58201 tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct
    58261 gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga
    58321 caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca
    58381 gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt
    58441 agcgtgcgcg tctccggaac tggcctctgg caaagtctgg atccgctatc caattgtacg
    58501 tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat
    58561 ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga
    58621 agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt
    58681 tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac
    58741 cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccgg tggttgaacc
    58801 gcaggaagag acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga
    58861 acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga
    58921 aacggtagcc gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat
    58981 gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg
    59041 ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc
    59101 ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc
    59161 cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa aattcatcaa
    59221 atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc cccggcgatc
    59281 ccagagcggg cagacaaaac atacccagtt tcatgatatg ttttaaagcg agcttatttg
    59341 ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga tgcctgcaat
    59401 gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc aaccagatcg
    59461 ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag cgactccagt
    59521 ttgttcagct gcgtgcctaa ttccgtgcga acggtaagga cattattcag cgagtttttc
    59581 agtccacggt tggttttatc caatgccgca gcggcgattt ctttatccgc ttcgctatcc
    59641 gcgaccggcg ttttcagggc tgcgatggcg ctatccagca tggcaaaaag attggtttca
    59701 gaagcgctac cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa aattttgtcg
    59761 cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat actttctgtt
    59821 ccgccgacat aatcaccgtt agcctcacta aacggcgcag tctctgtttt ataaccggca
    59881 aaaatgtagc gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc
    59941 ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg
    60001 tagacaattt tttcctgagc attctggata gcagtggtga cctggctaag tacactctct
    60061 tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc
    60121 tgtgcctggg agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg
    60181 actcgcttac ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg
    60241 atgccgcgca tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt
    60301 agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg
    60361 ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg
    60421 aaatcgactg ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg
    60481 ttttcaacgt cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa
    60541 aggatttcgc accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt
    60601 tgcggttgtc gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg
    60661 tgattaatac atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt
    60721 taacggcagg cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg
    60781 catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat
    60841 tatcgaacga gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac
    60901 cgatcgcaac gtcacctttg tttttcgtgt tttgctgaac cgcgggctta ccgatagtaa
    60961 agaaatcttc accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag
    61021 cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag
    61081 aacggaatgt cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa
    61141 tattgcctgc cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag
    61201 gaactgccgc cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt
    61261 gatgttataa gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc
    61321 gctcaccagt tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc
    61381 tgtcaggcgc gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg
    61441 atcaacgctg gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc
    61501 ggtggtttta aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc
    61561 cgggtcttcc gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt
    61621 tgccagcgaa gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg
    61681 ggcggtcagg ccgctacttt gcgtctgcac agcacgtaac tggttggtaa taaacgtatc
    61741 atactcacgc tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa
    61801 cgtgctattg gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct
    61861 ggagatatta ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc
    61921 gttattgatc aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg
    61981 gcagcgaccc gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt
    62041 ttgctcacct ggtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca
    62101 tagtgaggat cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc
    62161 gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac
    62221 aatgcttcca gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg
    62281 ttttcatatt cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg
    62341 acaccaaaca ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa
    62401 ccagattcaa gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt
    62461 gccagttggg cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa
    62521 tcatcgtagt tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa
    62581 cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat
    62641 ggttgttctg gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc
    62701 gtcatctgtt gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg
    62761 ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac
    62821 atcccttcca cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc
    62881 gcctttagtt cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg
    62941 ctgatcatca gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca
    63001 aaatagacat cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat
    63061 tgaggctggc gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct
    63121 gcgtttgtgg agtaacaaca gtctgtccac caccaaacgg tgtatctggc tggctgacat
    63181 tggcctgacg attaactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg
    63241 tcacttctcg attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct
    63301 gcggggtgac attaacctgc atattctgga tatcggcaag gaaacggacc tgggaactgt
    63361 tgccacttgg tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc
    63421 cacgcacgcg gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa
    63481 gttgcaaatt aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg
    63541 caccattggt gatccgtcca ccgttcagtt ggttaacctg aacactgcta ccgcccgcgg
    63601 aggctcctgc gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa
    63661 cgcccttaag cggtgtcatc aacaacgtac ctccacgcaa gcttttggca ttacccatgg
    63721 aagaaaccac cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca
    63781 ccattaccac agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca
    63841 gctgtgagag catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac
    63901 cagtgccatc cagccccact accaggccat agccaatcag tgagttttgc cttaccccct
    63961 gaacactggt gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa
    64021 ttaatgcaga gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa
    64081 gaaacgctgc aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc
    64141 aatacgcgca tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg
    64201 attaaccacg cccgagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc
    64261 acccaccaca tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc
    64321 gctaaaggta ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc
    64381 ttcgacatcg gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa
    64441 gccaaaatta gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac
    64501 gttctcctgc aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa
    64561 cagcggttga tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac
    64621 gggcgtcgga ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg
    64681 tatccaggcg cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc
    64741 agcgtttttt tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg
    64801 ttatgcctta gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac
    64861 tgttgatttc gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga
    64921 cgttagacgt ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc
    64981 tttcgttcgg tgcaccggag gattgcgttt cggtgtagag gttttcgcca atgctctcca
    65041 gtccggtatc attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct
    65101 ggccttgttg ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg
    65161 ccggaatggt gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct
    65221 gatccacctg gaaagagccg tcacgggtat aggctgatga gccatctggc aacattacct
    65281 ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt attggtctgc gacagattgc
    65341 cctggctgtg taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg
    65401 agggtaaggt ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca
    65461 gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt
    65521 tggcaatgac gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg
    65581 aactgatcat ataatgtcct gtagtggatt gctgacaagc agagcagaag cacgcgacag
    65641 tgtgaatttc ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct
    65701 tttattgcaa tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc
    65761 tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc
    65821 aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat
    65881 cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct
    65941 taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt
    66001 cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt
    66061 gccatcggca gcaatcgtga tttccgcccc ttccggcaca gcaattggcc ccgcctcgcc
    66121 tatcaccgga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt
    66181 acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg ctgcaacgca
    66241 acgtccagcg ggcgcgaggt gtaatccatc ttgccgggcg tcatgtctgc acccggtgtt
    66301 gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac tgcgcgcaag
    66361 gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg ccagattact ggcggttacc
    66421 gcctgttgat tcagcgtctg gctggctgct cccatcgcag tataaattgc gtgatccatt
    66481 gagctatccc gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct ggtcctgggt
    66541 tttgatggtc tgggcgttag actgatagtt acgctgggca acgatcatat tgaccagttc
    66601 tttactgaga tcgacgttgg acgcttccag cgcaccgttg gtcagggtgc caaagtttcc
    66661 cgtcccggct gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc
    66721 gccttcggat gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag
    66781 ttgggtttgt tcgttggaat agttgccgac aaccgtaccg tcatcattga tttgataact
    66841 caccagatcg cccggtttgt agccattctg ggtggttgcc acaatgttgt tagcgccggt
    66901 attttgctgc atggagttga ggaagctcag actaaacgtg gcgggttctg caccgttaat
    66961 tgcgccggtt gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa
    67021 ttccagtgtt gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac
    67081 ctgccagtta ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg
    67141 actgtcgaaa acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa
    67201 tggcgtaaca gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt
    67261 ggtagttttc gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg
    67321 ctgaatagtc ggcggcgtac cggttgccgg gtaacccgtc agctgtaaac cttgcatatt
    67381 caacaggtta cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc
    67441 gttgctgtct accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc
    67501 ggtgttggtg gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag
    67561 tcccactttc gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta
    67621 ggtggcggag ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa
    67681 tccgctaacc gcttgagaaa aggccatgac tgactcctga aatgtgaagg ctcaaattat
    67741 ctgccgtact tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct
    67801 gttgccgcgg atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt
    67861 accaccgtta ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc
    67921 agtcaacgta ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat
    67981 ggtgcgcaca accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg
    68041 ttgcagctca acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc
    68101 cgcaagaaca gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa
    68161 cgactggctg ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat
    68221 cccgctgacc gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt
    68281 ggtcgggtcc tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc
    68341 tgcggcgttg ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg
    68401 atcggtggtg gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt
    68461 ttcagcatca tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac
    68521 gccgacatgg tgttaaccat ctccccgacg acatcaacgt tcggcatttt tacgtagccc
    68581 tttgcatctg ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct
    68641 taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata
    68701 ccacctgttt tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca
    68761 gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat
    68821 caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt
    68881 tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact
    68941 gggtgacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    69001 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat
    69061 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    69121 atggtcattg cggacaggta caactcacgt tgtagaaatg gctgggggcg aaaatgctga
    69181 caataaaacg tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca
    69241 atctcacatc gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg
    69301 ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt
    69361 cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg
    69421 acaaacgata tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc
    69481 ctattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg
    69541 ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc
    69601 gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa
    69661 aagcgggaca acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag
    69721 gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcgggac
    69781 aggtagtcag cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta
    69841 aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg
    69901 aataaaccat gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc
    69961 gcgaaaccac tgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca
    70021 gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata
    70081 tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg
    70141 acaccggcaa aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact
    70201 gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc
    70261 aaaacggtaa tggatcatga gcagcaacat ctctctatgg ggcagatcaa ctgcagccag
    70321 ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag
    70381 ttacgcaaga aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa
    70441 attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa
    70501 ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg
    70561 ctatatgggg cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc
    70621 tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc
    70681 aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc
    70741 cagcacgacc gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg
    70801 catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg
    70861 caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca
    70921 gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg
    70981 tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc
    71041 aattttcact ggcgttttaa tgtcttggcg ggaataaaag ccaggagcca acactttcac
    71101 tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc
    71161 atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat
    71221 gcccaacgca accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat
    71281 caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa
    71341 aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta
    71401 atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa
    71461 ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat
    71521 tgctccagca tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg
    71581 cggtagctga tacaccagct gcaggatgcc gccgaccgta acagcccacg ccagcgccag
    71641 caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag
    71701 cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac
    71761 cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta gctggctggt
    71821 cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg
    71881 tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac
    71941 ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat
    72001 cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag
    72061 tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat
    72121 tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag
    72181 cgattttaat aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc
    72241 aggcgctgaa agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt
    72301 acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca
    72361 ctgcctattc gccggcggtt tgcggaactg tctgattttg cacgcattca atgaagtgcc
    72421 gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat
    72481 tcactacacc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg
    72541 caccgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag
    72601 tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt
    72661 cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg
    72721 tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc
    72781 tattgctacg atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact
    72841 caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg
    72901 ccgccagttc caccagcctt tcagcatcgc gcagattttc tgccagcggt ttatcgacac
    72961 agacatgtac ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg
    73021 agtgcacaaa aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa
    73081 tgcgccagct gtcacaaatt ggcagggctt tggcgcgcgt aggcgactag gctccttgta
    73141 acgtccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta
    73201 atcccactac gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa
    73261 tcaagacgtt gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa
    73321 tcaccgtcaa ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg
    73381 aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa
    73441 ggaccatctt cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg
    73501 aattcataca ttcgcgcggc gcggctgcgc agttcaccca gcgtctgggc accacgcaat
    73561 aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa
    73621 ttacaaaaac gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga
    73681 taatgacgtt tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg
    73741 accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcagg
    73801 tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg
    73861 gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt
    73921 taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata
    73981 gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat
    74041 ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt
    74101 cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata
    74161 gcaggtatga aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt
    74221 ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat
    74281 catccctagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc
    74341 ccagggcttg aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg
    74401 atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact
    74461 gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta
    74521 ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt
    74581 cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat attgtccccg
    74641 aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc
    74701 gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat
    74761 cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt
    74821 cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt
    74881 tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc
    74941 gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca
    75001 cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga
    75061 tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt
    75121 ctcgctgttg atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg
    75181 gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg
    75241 tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt
    75301 tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct
    75361 ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga
    75421 cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt
    75481 aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt
    75541 gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct
    75601 acaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc
    75661 gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt
    75721 tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt
    75781 tgacctgggc aaatcgacgc accagccaga gcttccgtgg atgatgctgg gaattattgg
    75841 catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt
    75901 gctggaacgc gacgcctgat ttgatacacc cccctgactc ctccatactg aaagtgaact
    75961 gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct
    76021 caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa
    76081 cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa
    76141 agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga
    76201 cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt
    76261 caatggctac gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt
    76321 ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt
    76381 tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc
    76441 gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat
    76501 tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg
    76561 cgttgctgat taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat
    76621 ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt
    76681 tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg
    76741 cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca
    76801 gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta
    76861 accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg
    76921 acgcccatat tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg
    76981 ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga
    77041 ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc
    77101 tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc
    77161 aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt
    77221 acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca
    77281 ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc
    77341 gcgcccttta tttttcgtgc aaaggaaaac atttccgctt atcctttgtg tccggcaaaa
    77401 acatcccttc agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc
    77461 gccgtccaga cgactggcac cttcacctcc gcgatggcga catgttacaa actgtcgtgc
    77521 cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga
    77581 ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc
    77641 acgatttcac cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg
    77701 agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca
    77761 ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg
    77821 aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt
    77881 ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg
    77941 cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg
    78001 gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata
    78061 tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc
    78121 accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg
    78181 attctgcgcc acatacacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca
    78241 acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc
    78301 actttgaagc attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca
    78361 cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg
    78421 acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat
    78481 cgtagcttcc tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag
    78541 ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct
    78601 attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa
    78661 ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa
    78721 gaagataaac agcgcattag cgaaattccc caggaaacgt gggaaagcgc cgatgactgg
    78781 tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg gcttttttat
    78841 tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg
    78901 ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt
    78961 tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg
    79021 cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc
    79081 catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag
    79141 caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc
    79201 cgagcaggaa gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca
    79261 atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa
    79321 cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc
    79381 tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag
    79441 cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc
    79501 cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc
    79561 accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga
    79621 aggcgaaaag tatgtcccgt tagtcctccg cgcgcaaacg ctgtgggatg aactttcccg
    79681 ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga
    79741 ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa
    79801 gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat
    79861 cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat
    79921 ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg
    79981 tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc
    80041 gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt
    80101 acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc
    80161 ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga
    80221 cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt
    80281 tccgtttgcg gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt
    80341 gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga
    80401 agattttatt atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag
    80461 tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga
    80521 ccaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt
    80581 acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat
    80641 ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca
    80701 attatggatc tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa
    80761 tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag
    80821 cactcaccag acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta
    80881 attcaaaatt attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt
    80941 cattctccga cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat
    81001 tcacaaatac ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa
    81061 ttattgtcta cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg
    81121 gctggtatca caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc
    81181 tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt
    81241 cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc attgtaccta ttgctgttcg
    81301 ccatcggcat cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg
    81361 gctggtttga cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg
    81421 ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg
    81481 ccctgaagca tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt
    81541 catctgacta tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc
    81601 tgatgttctc tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc
    81661 acgcctttgt taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta
    81721 aagatttcga cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg
    81781 tgacaattaa caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca
    81841 gtgcggattt tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga
    81901 agaaagacgg tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac
    81961 ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg
    82021 caggctttga ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag
    82081 gtccagcttc tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag
    82141 agtgaaatgt tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa
    82201 tacccagtgt tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac
    82261 gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct
    82321 cactacaaca acctttgtat ttttccgcgc atactggaca ctgaataaac agcaggtggc
    82381 agccatcatt tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg
    82441 cgataatctc atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa
    82501 taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct
    82561 cgatatgcca gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac
    82621 gaatgccgcc ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga
    82681 ctgcttttgg cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt
    82741 gccccacttc atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa
    82801 gcatggcgtt cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag
    82861 gatcgtcaat accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg
    82921 atttcccgtc atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg
    82981 catagagctg cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa
    83041 tgccctcatg cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata
    83101 aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg
    83161 ttcgcggttc gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt
    83221 gtaacactgg catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat
    83281 atagcaatta tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt
    83341 atttgttatt tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg
    83401 gcgaaaaaat gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg
    83461 acgaatctac ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg
    83521 ggtattggcg tactttggtt ggtcgtgcaa ttgccctacc cgattatcta ccgcctcggt
    83581 tgtggattag gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc
    83641 aacctggaac tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag
    83701 aatttcgaat ccgttggcat gggcctgatg gaaaccggta tggcgtggtt ctggccggac
    83761 cgccgaatcg cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg
    83821 caaaaacgcg gcatcctgtt agttggcatc cattttctga cactggagct gggcgcgcgg
    83881 cagtttggta tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt
    83941 gactggctac aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat
    84001 ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accagatcat
    84061 gattacggcc cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc
    84121 acgaccggaa cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca
    84181 cgccgtaagc cagatggcaa agggtatcaa ttgattatgc tgccgccaga gtgttctccg
    84241 ccactggatg atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc
    84301 atgatggcac cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc
    84361 gttccttcac gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc
    84421 acagcaaatc cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg
    84481 gcgcataatt agtgtgttta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag
    84541 cggattgcta tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc
    84601 gtcgcctggc taggctgttt tcttaccggg gccgccttca gtctggtaat gcccttctta
    84661 cccctctacg ttgagcagct tggtgttacc ggccactccg ccctgaatat gtggtccggt
    84721 attgtcttca gtataatggg ttcctaaaca catcatttta gatattgaat atgagacggg
    84781 caatcagcta catcagattt tcaagtgaac gacaactgaa aggggactct gtacgccgac
    84841 aatctaagct ggttactgac tggcttgata agaaccctga gttttactgg gattcatcct
    84901 tgagctttaa agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa ggtggtctag
    84961 gtgacttttt aaccgctatc gagaaagggc ttgttaaagc tggtgatacg ctactaatcg
    85021 agagtttaga ccgtctgtca cgtcaggata ttgatattgc ttctgagcta ttacgccgta
    85081 tcttaagggc tggtgtagat gtagtcacac tgagcgatgg agaacattac acacgagaaa
    85141 gtttaaaaga cccgttagcc ctgattaaaa gcattctgat aatgcagcgg gcgcatgagg
    85201 aaagtttgag gaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa gaactcatct
    85261 ccgaaggtat caaggtatct aggcgttgtc ctgcatggtt aaggctgaat gatgaccgtc
    85321 gaacattcac aatcattcct gataaagtgg aagttgttaa acgagcattt gacctgagat
    85381 tacagggttt aagcttttgg gctatcacca gaactttaaa cgatgaaggt cacttgtcat
    85441 tgaatcaata cacacccaaa caaaaggggt ggtctgatac tgctgtaaag aaactactca
    85501 gaaatcgtgc tgtgattgga tgtttcacac ctgctggacg tgaagaagtt cagggttact
    85561 accccgcaat aatcagtgag tcactattct atcgagtaca gcaactgaac acagggcaat
    85621 atggtagggc ttctgtatca agtaatccgc tatcagtcaa cttgttcagg ggtatcatca
    85681 aatgttctga gtgtggggct actattgttc ttggtggata tgccttaaaa cgttttggaa
    85741 tgtacagatg ccctaatcgt tcagctaaca ggtgtagtgc aaaggctatc agtcgtaagc
    85801 aaacggatgc aactatcctt tacatgctcg cattatgcga ccgttttgaa actgaaaata
    85861 ctgacactat tgatagcctt aaattacagc gtgaagattt gc
//
LOCUS       DADLXX010000002        82407 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLXX010000002 DADLXX010000000
VERSION     DADLXX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.002"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..209)
                     /locus_tag="JNX27_000095"
     CDS             complement(<1..209)
                     /locus_tag="JNX27_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7881442.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JNX27_000096"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JNX27_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7881443.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JNX27_000097"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JNX27_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7881444.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JNX27_000098"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JNX27_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7881445.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JNX27_000099"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JNX27_000099"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7881446.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JNX27_000100"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JNX27_000100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7881447.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JNX27_000101"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JNX27_000101"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7881448.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JNX27_000102"
     CDS             complement(4598..5320)
                     /locus_tag="JNX27_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7881449.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JNX27_000103"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JNX27_000103"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7881450.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JNX27_000104"
     CDS             6520..6738
                     /locus_tag="JNX27_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7881451.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JNX27_000105"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JNX27_000105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7881452.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JNX27_000106"
     CDS             complement(7472..7672)
                     /locus_tag="JNX27_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7881453.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JNX27_000107"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JNX27_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7881454.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JNX27_000108"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JNX27_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7881455.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="JNX27_000109"
     CDS             10169..12082
                     /locus_tag="JNX27_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7881456.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRQEAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="JNX27_000110"
     CDS             12082..13104
                     /locus_tag="JNX27_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7881457.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYLLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="JNX27_000111"
     CDS             13098..13316
                     /locus_tag="JNX27_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7881458.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="JNX27_000112"
     CDS             13370..14239
                     /locus_tag="JNX27_000112"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7881459.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="JNX27_000113"
     CDS             complement(14294..14698)
                     /locus_tag="JNX27_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY7881460.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="JNX27_000114"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="JNX27_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7881461.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="JNX27_000115"
     CDS             15683..17773
                     /locus_tag="JNX27_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881462.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JNX27_000116"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JNX27_000116"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7881463.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JNX27_000117"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JNX27_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7881464.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19741..20343)
                     /locus_tag="JNX27_000118"
     CDS             complement(19741..20343)
                     /locus_tag="JNX27_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7881465.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="JNX27_000119"
     CDS             complement(20333..20500)
                     /locus_tag="JNX27_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7881466.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JNX27_000120"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JNX27_000120"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7881467.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="JNX27_000121"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="JNX27_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7881468.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /locus_tag="JNX27_000122"
     CDS             22891..25434
                     /locus_tag="JNX27_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7881469.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="JNX27_000123"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="JNX27_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7881470.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="JNX27_000124"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="JNX27_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7881471.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWIALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cobA"
                     /locus_tag="JNX27_000125"
     CDS             26708..28081
                     /gene="cobA"
                     /locus_tag="JNX27_000125"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7881472.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="JNX27_000126"
     CDS             28328..28495
                     /locus_tag="JNX27_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7881473.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30127
                     /gene="frlA"
                     /locus_tag="JNX27_000127"
                     /pseudo
     CDS             28790..30127
                     /gene="frlA"
                     /locus_tag="JNX27_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30148..31170
                     /gene="frlB"
                     /locus_tag="JNX27_000128"
     CDS             30148..31170
                     /gene="frlB"
                     /locus_tag="JNX27_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7881474.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31220..32049
                     /gene="frlC"
                     /locus_tag="JNX27_000129"
                     /pseudo
     CDS             31220..32049
                     /gene="frlC"
                     /locus_tag="JNX27_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32046..32831
                     /gene="frlD"
                     /locus_tag="JNX27_000130"
     CDS             32046..32831
                     /gene="frlD"
                     /locus_tag="JNX27_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7881475.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32931..33662
                     /gene="phoB"
                     /locus_tag="JNX27_000131"
     CDS             32931..33662
                     /gene="phoB"
                     /locus_tag="JNX27_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7881476.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33822..34907)
                     /locus_tag="JNX27_000132"
     CDS             complement(33822..34907)
                     /locus_tag="JNX27_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7881477.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34919..36223)
                     /locus_tag="JNX27_000133"
     CDS             complement(34919..36223)
                     /locus_tag="JNX27_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881478.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36235..36588)
                     /locus_tag="JNX27_000134"
     CDS             complement(36235..36588)
                     /locus_tag="JNX27_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY7881479.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36599..37477)
                     /locus_tag="JNX27_000135"
     CDS             complement(36599..37477)
                     /locus_tag="JNX27_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7881480.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELV
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37474..38700)
                     /locus_tag="JNX27_000136"
     CDS             complement(37474..38700)
                     /locus_tag="JNX27_000136"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7881481.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38701..39864)
                     /locus_tag="JNX27_000137"
     CDS             complement(38701..39864)
                     /locus_tag="JNX27_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7881482.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(39948..40310)
                     /locus_tag="JNX27_000138"
     CDS             complement(39948..40310)
                     /locus_tag="JNX27_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7881483.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40327..41232)
                     /locus_tag="JNX27_000139"
     CDS             complement(40327..41232)
                     /locus_tag="JNX27_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881484.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41502..42506)
                     /gene="trpS"
                     /locus_tag="JNX27_000140"
     CDS             complement(41502..42506)
                     /gene="trpS"
                     /locus_tag="JNX27_000140"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7881485.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42499..43257)
                     /gene="gph"
                     /locus_tag="JNX27_000141"
     CDS             complement(42499..43257)
                     /gene="gph"
                     /locus_tag="JNX27_000141"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7881486.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43250..43927)
                     /gene="rpe"
                     /locus_tag="JNX27_000142"
     CDS             complement(43250..43927)
                     /gene="rpe"
                     /locus_tag="JNX27_000142"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7881487.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43945..44781)
                     /gene="dam"
                     /locus_tag="JNX27_000143"
     CDS             complement(43945..44781)
                     /gene="dam"
                     /locus_tag="JNX27_000143"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7881488.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44888..46174)
                     /gene="damX"
                     /locus_tag="JNX27_000144"
     CDS             complement(44888..46174)
                     /gene="damX"
                     /locus_tag="JNX27_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7881489.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46266..47354)
                     /gene="aroB"
                     /locus_tag="JNX27_000145"
     CDS             complement(46266..47354)
                     /gene="aroB"
                     /locus_tag="JNX27_000145"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7881490.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47411..47932)
                     /gene="aroK"
                     /locus_tag="JNX27_000146"
     CDS             complement(47411..47932)
                     /gene="aroK"
                     /locus_tag="JNX27_000146"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7881491.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48333..49571)
                     /gene="hofQ"
                     /locus_tag="JNX27_000147"
     CDS             complement(48333..49571)
                     /gene="hofQ"
                     /locus_tag="JNX27_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7881492.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQASSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49483..49887)
                     /gene="hofP"
                     /locus_tag="JNX27_000148"
     CDS             complement(49483..49887)
                     /gene="hofP"
                     /locus_tag="JNX27_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY7881493.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49877..50317)
                     /gene="hofO"
                     /locus_tag="JNX27_000149"
     CDS             complement(49877..50317)
                     /gene="hofO"
                     /locus_tag="JNX27_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7881494.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50301..50840)
                     /locus_tag="JNX27_000150"
     CDS             complement(50301..50840)
                     /locus_tag="JNX27_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY7881495.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50840..51619)
                     /gene="hofM"
                     /locus_tag="JNX27_000151"
     CDS             complement(50840..51619)
                     /gene="hofM"
                     /locus_tag="JNX27_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7881496.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51739..54291
                     /gene="mrcA"
                     /locus_tag="JNX27_000152"
     CDS             51739..54291
                     /gene="mrcA"
                     /locus_tag="JNX27_000152"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7881497.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54456..55016)
                     /gene="nudE"
                     /locus_tag="JNX27_000153"
     CDS             complement(54456..55016)
                     /gene="nudE"
                     /locus_tag="JNX27_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7881498.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55336..57471
                     /gene="igaA"
                     /locus_tag="JNX27_000154"
     CDS             55336..57471
                     /gene="igaA"
                     /locus_tag="JNX27_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7881499.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRQRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57536..58204
                     /gene="yrfG"
                     /locus_tag="JNX27_000155"
     CDS             57536..58204
                     /gene="yrfG"
                     /locus_tag="JNX27_000155"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7881500.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58215..58616
                     /gene="hslR"
                     /locus_tag="JNX27_000156"
     CDS             58215..58616
                     /gene="hslR"
                     /locus_tag="JNX27_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7881501.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58641..59519
                     /gene="hslO"
                     /locus_tag="JNX27_000157"
     CDS             58641..59519
                     /gene="hslO"
                     /locus_tag="JNX27_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7881502.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59654..61378)
                     /locus_tag="JNX27_000158"
     CDS             complement(59654..61378)
                     /locus_tag="JNX27_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7881503.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61757..63379
                     /gene="pckA"
                     /locus_tag="JNX27_000159"
     CDS             61757..63379
                     /gene="pckA"
                     /locus_tag="JNX27_000159"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7881504.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63455..64807)
                     /gene="envZ"
                     /locus_tag="JNX27_000160"
     CDS             complement(63455..64807)
                     /gene="envZ"
                     /locus_tag="JNX27_000160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7881505.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64804..65523)
                     /gene="ompR"
                     /locus_tag="JNX27_000161"
     CDS             complement(64804..65523)
                     /gene="ompR"
                     /locus_tag="JNX27_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7881506.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65715..66227
                     /gene="greB"
                     /locus_tag="JNX27_000162"
     CDS             65715..66227
                     /gene="greB"
                     /locus_tag="JNX27_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7881507.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            66324..68645
                     /locus_tag="JNX27_000163"
     CDS             66324..68645
                     /locus_tag="JNX27_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7881508.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQYPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69083..69310
                     /gene="feoA"
                     /locus_tag="JNX27_000164"
     CDS             69083..69310
                     /gene="feoA"
                     /locus_tag="JNX27_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7881509.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69327..71648
                     /gene="feoB"
                     /locus_tag="JNX27_000165"
     CDS             69327..71648
                     /gene="feoB"
                     /locus_tag="JNX27_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7881510.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71648..71884
                     /gene="feoC"
                     /locus_tag="JNX27_000166"
     CDS             71648..71884
                     /gene="feoC"
                     /locus_tag="JNX27_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7881511.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72087..72965
                     /gene="rpnA"
                     /locus_tag="JNX27_000167"
     CDS             72087..72965
                     /gene="rpnA"
                     /locus_tag="JNX27_000167"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7881512.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73038..73307
                     /locus_tag="JNX27_000168"
     CDS             73038..73307
                     /locus_tag="JNX27_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY7881513.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73301..73666
                     /locus_tag="JNX27_000169"
     CDS             73301..73666
                     /locus_tag="JNX27_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7881514.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73667..74455)
                     /gene="bioH"
                     /locus_tag="JNX27_000170"
     CDS             complement(73667..74455)
                     /gene="bioH"
                     /locus_tag="JNX27_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7881515.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74493..75176
                     /gene="gntX"
                     /locus_tag="JNX27_000171"
     CDS             74493..75176
                     /gene="gntX"
                     /locus_tag="JNX27_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7881516.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75235..75810
                     /gene="nfuA"
                     /locus_tag="JNX27_000172"
     CDS             75235..75810
                     /gene="nfuA"
                     /locus_tag="JNX27_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7881517.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76171..77475
                     /gene="gntT"
                     /locus_tag="JNX27_000173"
     CDS             76171..77475
                     /gene="gntT"
                     /locus_tag="JNX27_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7881518.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77520..79604)
                     /gene="malQ"
                     /locus_tag="JNX27_000174"
     CDS             complement(77520..79604)
                     /gene="malQ"
                     /locus_tag="JNX27_000174"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7881519.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79614..82007)
                     /gene="malP"
                     /locus_tag="JNX27_000175"
     CDS             complement(79614..82007)
                     /gene="malP"
                     /locus_tag="JNX27_000175"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7881520.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19763 a        22379 c        20788 g        19477 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttaggg cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcactcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccacgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tggaacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc agatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc
    11041 cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaggccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cagtcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcaggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccaaa accggaaagt
    12961 ctcccctcgc aggtggagta tctactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggaact gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgctg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctaggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttgc ttccggcact
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg
    17161 ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccctc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg
    18001 cccgaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatac ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt gcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tatgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtc gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc agctgtgagt tcccggcact gggcaaacgt
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaacat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcactccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtaaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tagcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggctcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gttcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacaca acatcatatg ttgttatatt catcatgcat tgtcatgttg ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtagct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29401 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29461 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29521 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29581 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggatctttgt
    29641 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29701 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29761 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29821 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29881 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29941 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    30001 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat
    30061 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30121 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30181 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30241 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30301 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30361 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30421 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30481 ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc
    30541 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30601 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30661 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30721 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30781 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30841 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30901 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30961 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    31021 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31081 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31141 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31201 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31261 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31321 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31381 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31441 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31501 ggcgatatat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31561 gggctatctc acgccgccta acgttatctg gggcggctgg cagagaactt aagcgaactg
    31621 tgtgagtacg cggaaaacat cggcatggat ctgatcctcg aaccgttaac gccgtatgaa
    31681 tcgaacgtcg tgtgtaatgc taatgatgtg cttcatgcgc tggcgctggt gccttcgccg
    31741 cgcctgttca gcatggtcga catttgcgcg ccgtatgtcc aggcggaacc ggtgatgagt
    31801 tatttcgaca aactgggcga taaattacgt catctgcata ttgtcgacag cgacggggcc
    31861 agcgacacgc attacattcc tggagaaggc aaaatgccgc tgcgggaact gatgcgcgat
    31921 attattgatc ggggctatga gggttactgt acggtggagc tggtgacgat gtatatgaac
    31981 gagcccagac tctatgcccg ccaggcgctg gaacgctttc gcgcgctgct gccggaggat
    32041 gagagatgaa aaccctagcg acaatcggcg ataactgcgt cgatatctac ccgcaactga
    32101 ataaagcgtt ttctggcggt aatgcggtca atgtggcggt gtactgcact cgctacggca
    32161 tacagccggg atgcattacc tgggtgggtg acgatgacta cggcacaaag ctgaagcagg
    32221 atctcgcccg catgggcgtc gatatcagcc atgtccatac gaaacacggc gttaccgcac
    32281 aaactcaggt ggaactgcac gacaatgatc gcgtttttgg cgactacacc gaaggcgtga
    32341 tggccgactt tgccctgagt gaagaggatt acgcctggct ggcgcagtat gacattgtgc
    32401 acgcggcaat ctggggacat gcggaagacg cattcccaca gctgcacgct gcgggcaaac
    32461 ttaccgcttt cgacttctcc gacaagtggg acagcccgct ctggcagaca ctggtgccgc
    32521 atctcgattt tgcctttgcc tccgcaccgc aagaagacga agcgctgcgt ctgaagatga
    32581 aagcgattgt tgcccgtggc gcaggaacag tgattgtcac gctgggtgaa aacggcagca
    32641 ttgcctggga tggcgcgcag ttctggcgtc aggctcctga accggtgacg gttatcgaca
    32701 ccatgggtgc cggagattcg ttcattgccg gattcctttg cggctggtct gcggggatga
    32761 cattaccgca ggcgatggcg cagggaacgg cgtgcgcggc gaaaaccatt cagtaccacg
    32821 gtgcctggta ggtataacgt tggcgtgagc atcttcacgc caacgtgctg ttacttgccg
    32881 gaaaacgacc ctataatccg agtaattcat tctttatttc agggtcgatt atgtcagcta
    32941 cggaccgcta ctctcatcaa ctcctctacg ctaccgtccg ccagcggctg ctggatgata
    33001 tcgcgcaggg ggtttaccag gccgggcaac agatccctac cgaaaacgag ctttgtacac
    33061 aatataacgt cagccgcatt accattcgca aagccatcag cgacttagtg gcagacggcg
    33121 tactgatccg ctggcaggga aaaggcacct ttgtacaaag ccagaaagtt gaaaacgccc
    33181 tgcttactgt cagtggtttt accgattttg gcgtctcaca aggcaaggcg acgaaagaga
    33241 aagtgatcga acaggaacgg gtcagcgccg cgccgttttg cgaaaagctg aacatccccg
    33301 gaaacagcga agtgttccat ctctgccggg tgatgtatct cgataaagag ccgctgttta
    33361 ttgatagttc atggatcccg ctgtcgcgtt atcctgactt tgatgagatt tacgtcgaag
    33421 gaagctccac ctatcagtta tttcaggagc gttttgacac gcgagtggtc agcgacaaaa
    33481 agaccatcga tatctttgcc gccacccgcc cgcaggcaaa atggctgaaa tgcgaactgg
    33541 gcgaaccgtt gtttcgcatc agcaaaatcg cctttgatca gaatgacaaa ccggtgcacg
    33601 tctccgaact cttctgccgc gccaatcgca tcaccttaac gattgataat aaaagacatt
    33661 aaccgtaggc cggataagat gcgccagcat cgcatccggc gatgctggcg cactgaattt
    33721 tacatcccgc acgttcccct caccctaacc ctctccccaa aggggcgagg ggagcgttca
    33781 gtgcggaatt tagcttgatt gccgaacggt gctatccagc attaaatact cgcaatactc
    33841 tcacgcaaaa tccgcagcac cgtctcttca ccgctgcgat tcgggttaat gcgaatcgca
    33901 caatgttcga gttgtggatt cgcctggcga aacgttcctg aaagtcgata gaagagcggc
    33961 gggatttcat atttcgactc tgcaccgacc gggtaaggca acgcgccacg cttttgcgct
    34021 tcttccagca ccctggcggc aatcagctga tgaaactcga caatcaacac cttcgactgc
    34081 gcattggcaa tgacggcgct tttcacttcc gccaccgcgc cgccgttaag caaagccagc
    34141 aaccgttcgg atactcccgc ctgcaccgca tgcatcactg gcgcgaacac cagaccacgc
    34201 aagacttcca gcgcctgtga gccctggatc tggctaccgc cggagtaaag cgtggcgcga
    34261 atccgactaa taacatcggc atcgccaacc actgcaccaa cgccctccgg cccaaacagc
    34321 ttgaagcagg agaatgtcga gacattcgcg ccgcattcac agccgattcg cgctaccttc
    34381 atcaccgcat agttatcatc ggttaacgct ggaacacctg ccgcgcgcaa cgttgccagc
    34441 acatctgcca gcacatagct gtcctgcggc tgctggcgcg tatgctgcac cagcgcggca
    34501 tcaggttgtt gctcgtcgac gacctgcttc agtgccaaca ggtcattgaa atcagcagta
    34561 ataagcgtca gccccatctg ctcaataata acccgtgtcg tcgggtaaac aggcgcgtca
    34621 tgcaccagaa gacgctgccc cggtttgagc aaagccgcca gcccggcgcg aatcgcgcca
    34681 gtccccgcgc cctgcaccag cgccgcggcc tgtgcgtgaa atgcatcagc cagcacctgc
    34741 tccacccgtt gggtaatacg cggttgattc agacctggag ttaaccctaa atcaccgcca
    34801 gcaagaaact cgctgcctgg aaaatggcgg caaatagtgt ccaccagcgc aaactgcttt
    34861 tgctgcgctt cagcaagagt caggctttgc aaaggaaacg tcttcatagg agccttcctt
    34921 aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta
    34981 ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt
    35041 aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg
    35101 aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg
    35161 cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt
    35221 ccgccgaaat aaccactgcg cctaataccg ctgcaaccat cggattcggc gagagatagc
    35281 ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg
    35341 tggcaatcaa cggcacaaac cccagtccgc gcataaattc tgccagagcc gcctgattaa
    35401 tcagcgtttg cgattgttcc ggcgtcacgc ctgcggaata cgctttctcc agtgtgaaga
    35461 tcgaaacttc gctaccagca aaaatcttca tactggcaac ggcggcaatc aatgctccca
    35521 cgatggcgat ataaggtaag tttttgataa tccgcgacgt gcgctcttca aacaccgaaa
    35581 gcccgctggc atcgatgtca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc
    35641 ccagcagcat caccatgcca ataaagattt caatggattc agggttaaga tgtgggaaat
    35701 agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc
    35761 agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa accaccggcg
    35821 agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc agctggttaa
    35881 ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca
    35941 ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac catgcgttga
    36001 gcagccaggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac
    36061 caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga
    36121 tcgggtggat gccgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac
    36181 ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt
    36241 cagatggtta atcaacatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc
    36301 cactttccct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt
    36361 ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc
    36421 cgcaccggta ttacacgcgc caatgtaata atctagttgc ccggatttca ccttcattgc
    36481 cgcttccatg tcgttgtgaa tgaaaacttc aaaacagcca ggagccgtcg cttcaatggt
    36541 ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt
    36601 attggaaaaa ttgagaggga ttttcacgta acatcacatc cacatcggcc tgactgaatc
    36661 ctgactggca caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat
    36721 tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc
    36781 cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag taactgttct
    36841 taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga
    36901 ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt
    36961 gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg gagatcgggc
    37021 gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa
    37081 tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc gtgccatcga
    37141 taccctgttc aatttcatcg accatctcct gcaccagttc ctgcacgctg cgggtcacca
    37201 catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg ttgatcccgg
    37261 tttcgcgcat tacatcgagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct
    37321 caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat
    37381 actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct
    37441 catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag
    37501 ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt
    37561 agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtggcgacca gcccttgctg
    37621 ataaaccagc actggcacca cttcacgggt atggtggctg tgaccaatgg tcggatcgtt
    37681 accgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcctcaa caagccgggc
    37741 aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc
    37801 agcgaggtcg gtttcctgga tattgatgca aataaacgcc gtcggatggg tgttaaattc
    37861 gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc tcacaccata
    37921 aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcttcata
    37981 cagtttttgc ggcacctgca ctttttcatc aacaccgtag cccatatgca cgacctggaa
    38041 gccgttgtca taagcgccag aacgcggcgc attaatacca ataaagcgcc cttctttgct
    38101 ttctgcggca tcgagaatac gctgactgtt ggttaacagg ccgccaaatg taatgacccg
    38161 accgacctgt acctggtcac gcacgatacg accaattttg attgcgtcgt caaaagagat
    38221 cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga gattatcgcc
    38281 aatcgcaacc gcctgattga cccacagaaa ttgcagatca tcgccacggc gctccacctg
    38341 ccagccagcg gaaactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg
    38401 cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc
    38461 ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca tatcgcctgg
    38521 cgcataaccc aatgtgttga ttagccccag cttctccaac gttggtagct gcaaatgcgg
    38581 caactggctc aggatgtgac cacaggtatt cgccccggca tcttgcggac gcaccagcgt
    38641 gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat
    38701 ttatgccttc agagggttgc cgaggctgtc ataacgacca actatttctg gttcgccgcg
    38761 ctgaataccg gacaccagca ccacatcgct acgggtgaca aaaatctgcg tgcgaaaaca
    38821 gagcaccact gcactgctca ccggatactc gcctgccagc ggcagggtgt agtcgatact
    38881 gctgtcatcc acagttttga gattggtttc agtaatcttt tgattttctg gcgtaaacac
    38941 cagcgcatgt tgcgcatgac cacgacggta gtaaccgccg ccgtagcagt agctgtcgcc
    39001 acggaaatga tgggagattt cacttaacca gagcatcgcg atacgttcag gctgatcgcc
    39061 ctgctggttt gccggaatag taccggtcag cgcatgaccg ggttcggcat gagtcgcacc
    39121 gtattccgcc agcaatggca gcgaagtgca gctggtcgct gaaggtacgt tcagttgctc
    39181 aatcgcaata ccagatttcg ccagttgatc ccgtgcttgt accagcgtgt gaagattcgg
    39241 tgttggcaaa actttcccgg cagcctcatc ccaaagcagg caagggaaat gggttagtcc
    39301 ggctaaatgc agccccggca gtttcttaat ttcagcaatc acctcgtgca gcgaatgctg
    39361 tacaaaaccg ctctcctgcc ccggataaag aaaatcatcg tcgctataaa ctttgagcag
    39421 cacaggctgg acccgtccgg ccttaaccgc tgccgcagaa acttcccgcg ctttgtcgag
    39481 agtaaacacg gtgatgatgt cggtgccctg ttcaacggcg tcagcaacct gatgacaagg
    39541 gatttgtacc agatgcccct gatgcgccac tggcaaacca gcgcggcgca tgactcgcgc
    39601 ctctttgtaa tccaccgcca caatgccgct gtagcctaat gccagcaatt tttccgccag
    39661 ccacggatta cgaccaaatt gcttggtcat cagatacagt tcaatgccgt aaagccgcgc
    39721 cgtctcaatg agccgcttac cgttttccag tatctgatcc acgtcgatca cccagctgtc
    39781 cggggcgatt ttgccctgct gccacaaact tagtgcggcg gaaatcagcg ccgggttctg
    39841 gcgtttcaat gcttctacaa acatcttgtt gcctcgtttc tgttccttga attaaatatt
    39901 cattttttta aatatttagg ttgtgcgacg tggatttgtc gggagcttca aacctcttcg
    39961 ttagcagcca tccataatcc ataaagattg gccagaaaat agccttcttc acacgggtta
    40021 acttccagag cgaattcctt caacaacacc tgatgcaatt gcacaacggc ttgccagtgg
    40081 ctggattgcg ccagttccgc cagcaactcg ttatccagcg gctctatttc ttcaccacgg
    40141 cgactgcgca tcagtgcact cgccatatgt gtcatcgcca tcgttccttg ctcactgcgc
    40201 accggcagat ggcactcttt ttccagtacg ttgacgacct gcatcatgcc tttgcagacg
    40261 tccttatcaa taacgcctgc ctcgcaaagc aggttgagtc tggtttccat atcagatgcc
    40321 tttcgtttaa taactgggtt cctgttcccc actcaccacc cgttgttgat gggcaagcag
    40381 ggcgtgttta tctacaacgg cacgcagcaa ttgttgcatt gggtaatcat cgactcgcgt
    40441 caggaccacg gcttcggtgg ccagtaaaaa tcgcggatcg tctgttagcg gcgtcgcctc
    40501 taatcccagc atggtcagtt cgttttccgc caccacgttc cagatcaccg catcgacatc
    40561 gcctttaaca atgcgttgta aactctcgtg ataagagaga tcgactcgtt ccacatcact
    40621 atcgccaaaa aagacatcgg tcatgatttt ctgatccgcc gaacgactat cgagacctac
    40681 tcgcttaaca tttccagact cacctttgcg gcatatcagt tgatgttcac caacatacgt
    40741 atgcgggcca agctcgaggg cgatgcacaa attgttttgg ctgagataac tttccgccgc
    40801 caggcgggaa actactgcca tgtcatagac accgttaagt aaacactcca cgcgaatatc
    40861 cgcgccgcgc atatgcgcat agtaaaaagg aatgccatca aactgggctt tcaatccgct
    40921 cgctaaacct tcgtacaagc gggtataggg caaaggcatc gcacatacca cgttgttgat
    40981 atccacatga ctcagcaatg ctttgttatc catctcgaca agataactgc cattgcgccc
    41041 acggcgttca atccgtatcg ctccgcttga ttccagcgtt ttcaacgcgg cctgcgtcag
    41101 gccaaccgat gaacggcatt cagttgccag ctcatctatg gttttcagtc gattaccgca
    41161 tttttcaccc aacaaataac gggccagtgt cgtgatgacg acgccttctt ttttgataaa
    41221 cgttcgacgc ataatatatt ttcaataaaa tgaatattta tatcttcatt aaaatgaaga
    41281 taagtggcga cgacgagatg tggaaacggc gaggcgcttc acattttttc gggttgttgc
    41341 gaaaagagga taaaaaaacc gggtttcccc ggtttcagag tgatgacgaa gtgatttatt
    41401 cgacaatgcg agcaaagggt aattcccact ggcgcttgcc tgctcccctc accctaaccc
    41461 tctccccaaa ggggcgaggg gaccgtccga gcgttttgat attacggctt cgccacaaaa
    41521 ccaatcgctt cgtacaccgc tttcagcgta cgggaagcgt gtacactggc tttttctgcg
    41581 ccgtctttca tcacctgttg caggaaggct tcatcgttgc ggaaacggtg atagcgttcc
    41641 tgcaattcag tcagcatacc ggaaacggca tcagccactt cacctttcag atgaccatac
    41701 atcttgcctt cgaactgttt ttccagttct gggatgctct ggcccgttac cgcagaaagg
    41761 atatccagca ggttggaaac gcccgctttg ttctgcacat cgtagcgaac taccggcggc
    41821 tcgtcggagt cagtgaccgc gcgtttgatt ttcttcacta ccgatttcgg atcttccagc
    41881 aggccgataa cgttattgcg gttatcgtca gacttggaca tcttcttggt cggctccagc
    41941 agcgacatta cgcgcgcgcc agatttcgga ataaacggct ccggcacctt aaagatctcg
    42001 ccatacagcg cgttgaaacg ctgggcgata tcgcgactca gttccaggtg ctgtttctgg
    42061 tcttcaccca ccggtaccag attagtttga tacagcagga tgtccgcagc catcagcacc
    42121 ggatagtcaa acagaccagc gttgatgttc tcggcataac gcgcagattt atctttaaac
    42181 tgcgtcatgc gactcagttc gccgaagtag gtatagcagt tcagtgccca gcctaactgc
    42241 gcatgttctg gtacgtggga ctgaacaaaa atggtgcttt tctcaggatc gataccacaa
    42301 gccagataca aggccagcgt atccaacgtc gctttacgca gcttctgtgc atcctggcgc
    42361 acggtgatcg cgtgttggtc aacgatacag taaatgcaat ggtagtcatc ctgcatgttt
    42421 acccactgac gcagcgcacc catgtagtta ccaatggtca attcacctga gggctgtgcg
    42481 ccactaaaaa cgatgggctt agtcattttt cgattcctga ttttcgctat gcggaagccc
    42541 taatgcgggc agaaggtcat ttatagactg ataaattaca tcaggctggc tgagatcgat
    42601 agcctcgccg tagttatatc cgtaggttaa gccaactgat gggcaacctg ccgcttttgc
    42661 cgcctgaata tcattgcgtg agtcgccgac aaacagcatc tgttgtgggg caattcccat
    42721 ccgctcagcc accagtaaca gcgggtccgg atgcggtttt ttgttttgca catcatcgcc
    42781 gccgataacc acgctaaagt atttggcaat atctaaggct tcgagcagcg gcgcgacgaa
    42841 cggcgtcggt ttgttggtga ccaggcctag cggcaggcct ttagcctgca acgcgcccaa
    42901 cgtatcggca acgtgcggga acaaaaacgt cccctcttcg gcaacctcgc catagtagcg
    42961 atcgaacagt ttacgcagaa tacgtacctg ttcttctgcc ggaatgtcgt catcaacggg
    43021 cggtttcccc attgttttac gcagagtcgc acgttcctga cgcgcccagg tcaatgcgcg
    43081 ctccatcaga acatctgcgc cgttaccaat ccaggtaata acgcgttctt cacctgcgac
    43141 gggcaactcc agcgcataca gcgccatatc taccgcagca gcaagaccag gagcactgtc
    43201 gaccagcgta ccatcaagat caaaagcgac gccgcgaata tcttcaaact tattcatgac
    43261 ttacctttgc cagttcactg cgcatttcat caatgacttt tttatagtct ggctggtcga
    43321 agattgccga accggcgacg aacatatccg cgcccgccgc agcgatttcg ccaatgttgt
    43381 tcaccttcac gccaccgtcc acttccagac gaatgtcaaa gccagactcg tcgatacggc
    43441 gacgtacttc gcgcagttta tccagtgttt gaggaatgaa agactgaccg ccgaaaccag
    43501 ggttgacgga catcagcagg atcacatcca gcttatccat cacgtaatcc agatagctca
    43561 gaggtgtcgc cgggttaaat accagacccg ctttacagcc attttcttta atcagttgca
    43621 gcgtgcggtc aacatgctcg gaggcttctg gatgaaaggt aatgatgctg gcaccagcgg
    43681 cagcgaaatc aggcacaatg cgatcgacgg gtttcaccat cagatgtacg tcgatagggg
    43741 cggtaatgcc gtagttacgc aaggatttca gcaccattgg cccaatcgtc agattgggaa
    43801 catagtggtt atccatgacg tcaaaatgca cgacatcagc gccagctgcc agggcttttg
    43861 cggtatcttc acccaggcgg gcaaaatcag ccgacagaat tgagggggca atcaaatact
    43921 gtttcatccg cttctccttg agaattattt tttcgcgggt gaaacgactc ctggtttgta
    43981 caaagccagc agttcgtcca cctttttacg tgtgccgccg ttgctgctta tactgcgtcg
    44041 aactttgacg acatgcaatt ttgcgcgctg ataccactca cgcgttaaca tcgtatcgtg
    44101 attggagatc agcactggaa tatggcgctc aaccagacct tcggcgatct ccgccagatg
    44161 cgcttgttgt tcaagcgtaa aactgtttgt gtgatacgcc gtaaagttgg cggtcgcaga
    44221 cagcggtgca taaggcggat cgcaatagac gacggatgca tcatctgcgc gctccatgct
    44281 atcggcgtaa gactcacaat agaaaaaggc attctgcgct ttttcagcga agtgatacaa
    44341 ctctgcttcc gggaaatagg gttttttgta gcggccaaac ggcacgttaa actcaccgcg
    44401 cagattgtaa cgacacaggc cgttgtaacc gtagcggttc aaatataaaa acagtaccgc
    44461 ccgacggaac ggatcctggc ttttgttgaa ctcttcgcgg aactgatagt aaacctcggc
    44521 gcaatttgtt tcgggaacaa acagctcgcg tgcggcctgt acgtactcat cagtacgcat
    44581 cttcacaatg ttatagagac tgatcaggtc gctattgata tcggcaagga tataacgaga
    44641 aaagtcggtg ttgagaaaca ccgacccggc acctacaaaa ggctcaacca gacattcgcc
    44701 cttgggcaaa tgccgtttaa tatcatcaag caggggatac ttgccccctg cccacttcaa
    44761 aaaagcgcga tttttcttca tgccgactaa ctaattacac cttctccggc tgtggagaaa
    44821 gctccgacag catccagcgc ttcaggcagc tcccgcaacg ataaaccatt gcgggagata
    44881 accttgatta cttcagatcg gcctgtacct gacgcagcgg tttcgcccac gggtttttgg
    44941 cctgaacatc tgctggcaat gtagataccg cttttttcgc ctcttctttc gaagcgtaca
    45001 cgccagaaac caggacatac cacggctgac cattacgcgt cgtttcatag acaacgtagt
    45061 ttttcagatt ctctttcttc gcccaaccgt tcaggttgtc gtagttagag gaactgctca
    45121 gctgcagagt gtaatggctg gacggtgccg atttcaacga accaacatta cctgcggtct
    45181 tccctccagc ggctggtgtt gccgtggttt gcgccgggga tgccgtctgt actggagccg
    45241 tagtcgccgt ctcttttggt gctggcgtag aagtcgcagc cggtgccttc gtgctcgcca
    45301 caggggcagc tggttcagta cgcttcggcg tctgtgctac cggcttcggc tccgttttca
    45361 cggttgcttg cggttttttc ggttcaatca ccgcctgctg acgaactggg cgcgtagtgg
    45421 acggacgttc agcggtttgc gttttcgccg tgtcacgcga tgcattgcca ttgcgaacag
    45481 gcgcaaccgt tgcgggttca gtcggcaacg tggaattgac cgccacattg ttcagctgtt
    45541 gctgattttg tggctgggtc agcgcattgt tcaggtcacc ctgcacttca acacgttgtt
    45601 gaccatccgt tgccaccggg gtttgccctt gagtcggcgt agaagagatc ggtggcagag
    45661 aaacatcctg ctgagtattt tccgcagacg tggttcccgg cgctggctgc acgccattcg
    45721 cctgatcggt cgcattgcca gcaagatcaa tactcttctc gccagacgcg gtttgatcgc
    45781 tggaagtggt cgagggggct tttagcgcag aaccgatacc gatgatcaac agcagtagaa
    45841 ccagaatgcc gacgcccatc atcatatact gacgagaagc gggtttactg gctgcttttt
    45901 tgcgcttacg cggacgacgc tctacgcgct cttcatccac ggtttcatct tcggattcgt
    45961 caatttcttc ttcgatttcc ggttcctcat tgcgctcttt ttgcgcacga gtcggacggc
    46021 gatcgtcagt gtcatcaagt tcaatatcat caaaattgat ctgcggttcg ccacgttcag
    46081 tacgctcaga acgttcagaa gattgacgag aacgaccagt acgacgatcg ctgggatcgg
    46141 gtttcagctc gtcttctggt ttgaattcat ccatttaaca ccccactaaa aggttaatgc
    46201 ttaccacgtt gcaattaacc tgaagctaat agaccgcttg ataagcggcc tgacctttct
    46261 tgttgttacg ctgattgaca atcggcaatg gcgttaagaa caagctcgtg cgaaacgccg
    46321 ctgcgaactt cactcttacc aattgccaac ggaagaatta agcgcatctc tcccgcaagg
    46381 actttcttgt cacgcagcat atgcggtaaa tacgcctgcg cggacatttc gcgcggccca
    46441 ttgaccggta acccagcccg cgtgagcagg gttataatac gctgcgtttc ggcagaacta
    46501 aactgcccga gacgttccga cgtccgcgcc gccatcacca tacccgcagc gaccgcttca
    46561 ccatgtaacc aattgccata ccccatttca gcttcaatgg catgaccaaa ggtgtgtccc
    46621 agattcagta aagcacgtaa cccggtttcg cgctcgtcgg cggcgacaac ttctgccttc
    46681 agttcacaac aacggcgaat acagtacgcc attgccggac cgtccagacg caacaacgca
    46741 tccagattct cttccagcca gttaaaaaac gcaccgtcaa gaataatgcc gtatttgatg
    46801 acttctgcca gccccgacgc taactcacgc gggggaagcg ttttcagaca gtcgagatcc
    46861 accaccactg aagcaggctg gtagaacgcg ccaatcatgt ttttaccgag gggatggttg
    46921 accgcagttt tgccgccaac ggaggaatcg acctgcgaca gtaacgtcgt cgggacttga
    46981 atgaaacgaa caccgcgctg ataactcgcc gccgcgaagc cggtcagatc gcccactacg
    47041 ccgccgccaa gcgccaccag cgtagtatcg cgaccatgcg gcttttgtaa caacgccgta
    47101 aagacggtat cgagtacagc taggctttta tactgctcgc cgtcagggag gataacgcta
    47161 tcgacgttaa cacccgcctg ttcaagtacg ccgcggacct tatcgagata cagaggagcc
    47221 agggtttcgc tggtgaccaa catgacctgc tcgcccgatt tcagcggtaa gaatgaagct
    47281 ggttcattaa acaaaccaga tgcgatggta attgggtaac tacgttcccc gagagtaacg
    47341 acaatcctct ccataacgcg acatccacct taattactgt acccgcagac gagtgtatat
    47401 aaagccagaa ttagttgctt tccagcatgt gaataatctg gtttgcaacc actttagcgc
    47461 tttgatcatc agtacgaatg gtcacgtcgg caatctcttc atacagcgga ttgcgttcat
    47521 tggccaacgc ttccagaact tcacgcggcg gtgtttcaac gtgcagcaac gggcgttttt
    47581 tatcacgctg cgtgcgtgca agttgctttt cgatggtcgt ttcaagataa acgacaacgc
    47641 cacgagcgga aagacggtta cgcgtttcac gggatttcac agagccgccg ccagtagcca
    47701 gcacaatacc ctgtttctcg gtcaactcat tgatgacctt ttcttcgcga tcgcggaagc
    47761 cttcttcgcc ttctaaatcg aaaacccagc ccacatcagc tccggttcgt ttctcaatct
    47821 cttgatcgga atcgtaaaat tccatattga gtttttgagc taactggcgc ccaatagtgc
    47881 tttttccggc acccataggc ccaaccagaa agatattgcg tttctctgcc attttttcgg
    47941 tactactaag actattcgtt aatgataaac ccgcttcgct cagagagcgc cgcaggacat
    48001 gaactgaaac ctcataagat attgcgagag tcagactgaa aattatctca atactccagc
    48061 gggtttggca actgaataaa tcacccatcc ctctgcatat ctggtcgctg caagcgcact
    48121 gccttgctac caccgctctg gcgataaatc accgggtaag attagcgtaa aaaagacagc
    48181 aaaatgccgc ctgaatgata aatcatcatc atggaacgca ggcagcgaag agcaccaact
    48241 ctcaaatcgg tactccttgt atgctaaata cctgcgcgcg tcaaatagat gaaacacgtt
    48301 cagcataaaa acattaccgc ttttacggct gtttactcac tggaaaccag tcgtggcgtg
    48361 ataaacacca ctaactcgcg tcgttcatct tcttttccgt catgacgaaa taattgcccg
    48421 aaccagggaa tgtcgccaag caacggtacg ctatcctgac ccgatttatt tttacgggta
    48481 aaaatgccgc ccagcgccaa cgtttctccg ctttttacct cgacctgcgt ttcgatctcc
    48541 tgcttatcaa tcgccagcac ttcgccatcg gcctgctgta gcacctgccc cggaacgttc
    48601 tggctgatgt gtaatttcag ccggatgcga cctttttgta acaccgtggg cgtgacctcc
    48661 atccccagga cggcctcttt aaattccacc gacgtcgcgc cactttcccc gctggaagcc
    48721 tgatatggaa tttcgctccc ctgtttaatg ctggcaggct gaagatgtga ggccagcaga
    48781 cgcggactgg cgataatatc cagctgctgt ttttgttcga gcgcggaaag ctcaagatcc
    48841 agcaagcgtc cgttgatgcg cccaatgtta aaaccgacat gcgttgtcgc cgtcgctacg
    48901 gagaggtcgc taccaagcgt ggtgacttgc ccaacgccac cagcgtgttg cgcatcggcc
    48961 agcgtccatt tcacgcctaa ctcacgcaaa cttttttcat taatggtgac aatatgcgcc
    49021 gacagctcaa cctgcccgac cggcagatcc atttgcgcta cccactgttc aagcgcgctt
    49081 aacgccgttt tgttatctcg tagcaaaagg cgattggtgc gtttatcgac ggtcatactc
    49141 cctttggcac tcagtagctt ctcccccgct ttcgccagtt ctcccgcgtc ggcgtattgc
    49201 agggttatac tgcgattttc cagcggcaga tttgcctgcg cccgcgcctg ctccgcctcc
    49261 tggcgggcga tattgtcatt ctgccaggca atggaatgca ctgagagaat gttgccttcc
    49321 tgccgcgtta tcagtccggc gcttttcact acagtttgta gtgcctgctt ccagggaaca
    49381 tctgttagat gtaacgacac cgtaccgctg acgtctggcg acacgaccag gttcaacttc
    49441 tcctgttcag ccagcgcctg caacacctga gctaccggaa cgtcatccac catcagcgtc
    49501 actttttgcg gctttgccgc ctgtacgccg ggtatcagca tcaacagtag tgcggctatc
    49561 cattgcttca tttgtatctc cttgccgttg ccacaaccat tgtggcggtt cgcagtttgt
    49621 cccggtaccc agcgttagta cgtctggcgt cagctgtaaa attgtccagc cgttttccag
    49681 cacatcgttt tgctgcaccc gtcgccattt cttttgcccg tcttttatta caccgatgat
    49741 gcgctcgcct cgccctacca tcccctgata gcgccactgg ctaagttcgc taatccggca
    49801 tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca atgcaatacc
    49861 tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg aacaaaagat
    49921 catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct gccagccgtg
    49981 taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcgcct ccctgcgcgg
    50041 atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc gaaaagggca
    50101 gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg gcccactgct
    50161 ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt gtcgatgata
    50221 gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg cggaggcggg
    50281 gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct tgttaactga
    50341 tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg attgagatga
    50401 aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat gcttgttgtc
    50461 agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca ggcgtgctcg
    50521 ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa ttgtcgctgg
    50581 cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg tggcttcgtt
    50641 atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc tatgcgcgct
    50701 tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag cagaggcgca
    50761 acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg ccagggcaaa
    50821 aaattaattg gcgggttcat cagtacgcct tcccaagcgc cagccccagc gcgatggcaa
    50881 agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc caggggtcaa
    50941 atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct gccgccagct
    51001 ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc gtcgcccaca
    51061 gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa aaaggcagga
    51121 atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc aacctttctg
    51181 ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta taagcggggc
    51241 tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc agctcgcggg
    51301 ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc gacgggcgcg
    51361 gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga tgacgctgcg
    51421 gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca acaatccgcc
    51481 catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa aagcattctt
    51541 ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc aaaccaattt
    51601 gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg acgggtatat
    51661 caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc ccaaatgaaa
    51721 ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc agtctgttgc
    51781 attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc acaactgccg
    51841 gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta cagcgccgat
    51901 ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt ggatcaaatc
    51961 ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta cgagcatcac
    52021 ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc cggccacgcg
    52081 tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct cagtccagaa
    52141 cgcacgctga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga acagttgctg
    52201 acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta ccgcgcctat
    52261 ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact gacgctgaac
    52321 gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc gctctactca
    52381 atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga tgaagggtat
    52441 atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa ttatcacgcg
    52501 ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga gatgtataac
    52561 cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat cacccgcaaa
    52621 gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga catgcgccac
    52681 ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg ggataacaac
    52741 aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc tgccgcagtc
    52801 accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac cgtcgcattg
    52861 agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca gggaccgacg
    52921 ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg tcaggttggc
    52981 gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc gatcaatccg
    53041 caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag caagtttaac
    53101 cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct ctacaccgcg
    53161 gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat ttctcgctgg
    53221 gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta tgctggtcca
    53281 attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg tgcaatgcgg
    53341 gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc ggcgcaaaac
    53401 attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat gcaggtggcg
    53461 cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt tatcagcaaa
    53521 attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc ctgcccggaa
    53581 tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga aaataacgat
    53641 gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat gccgcagctg
    53701 gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc accgcacgtc
    53761 atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat ctttggtgaa
    53821 ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg cgatatcggc
    53881 gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta cggtccgggc
    53941 gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg tcatacaacg
    54001 gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa gagtgcccag
    54061 cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca gccgctgacg
    54121 ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt agctaatggt
    54181 ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca ggcggtgcac
    54241 gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg attaaaaagg
    54301 cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg atgcgggtga
    54361 acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac cccataagcc
    54421 tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg ccctttcaac
    54481 cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc agggtcttcc
    54541 agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc tggctcatcg
    54601 ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt actggaaaag
    54661 taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc aaatccaacc
    54721 tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc aattaatcct
    54781 ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat caggatcagg
    54841 tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg cattcgttca
    54901 taaacacgcc gcacgccatt gctgaactcc agatccacac tctcgacggt aaacagtcgg
    54961 gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt gttcatcgtg
    55021 ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt cggcaatgtg
    55081 aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa tcaaattaaa
    55141 agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag agggtttgct
    55201 atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaaactcca cgcttgccga
    55261 tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg acagactaag
    55321 taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct ggcctgctca
    55381 ctacttgcgg gatggctgat aaaagtgcga tccagacagc gtcagctgcc ctggaccaac
    55441 gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc cgttgaaaat
    55501 tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacgggagc tagcgcggca
    55561 ccgatctctc tggtgctgaa tgccgaaagc aacaacgtca tgatgctgac acacgctatc
    55621 acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct cgattcggta
    55681 gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac cgttgaactg
    55741 attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct gcaggagtac
    55801 atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc gtcgattcgc
    55861 ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca tgaagaatat
    55921 gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc cttcctgatg
    55981 ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg cggcgcatta
    56041 ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc ctccctgcga
    56101 gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga aaacgatcag
    56161 gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc acactggcag
    56221 ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct cgaccgccac
    56281 gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt tccgttacag
    56341 cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tggtgctgtt tatgctgtta
    56401 ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa aggcgcgcag
    56461 accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt aggcgataca
    56521 ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg gagcgcgaaa
    56581 accaattcac cttttttacc atttgactgc tcgcagatca tctggaatga cgcccgctca
    56641 ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga agcagttaat
    56701 cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt acgttcagcg
    56761 attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact gaagacagcg
    56821 gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt caatctcggc
    56881 aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ctgggaagct cgatggcgtg
    56941 aatgtgttat tacgcccggt gagtgcggaa tcgctggata acctggtggc aacctccacc
    57001 gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag tccggccccc
    57061 ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc ctggccttcg
    57121 gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa actggcacaa
    57181 atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt cactgacgcc
    57241 aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct gtggcgctat
    57301 ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa tggcgtacag
    57361 gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc ctattatgaa
    57421 agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata acacgtttgc
    57481 gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt ccctcatgca
    57541 tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg gcacgttgct
    57601 cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat ggggcgcgaa
    57661 aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc acgacgtaca
    57721 gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc tggatatctg
    57781 tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca ttccgtttct
    57841 tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc atccgcacaa
    57901 cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat tactttccac
    57961 ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg ccgaagctac
    58021 gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc tcgatgctgc
    58081 cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg ggattgccga
    58141 gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc cctcgctaat
    58201 gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat aaatggctat
    58261 gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattaaa ggcggtaagg
    58321 tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat gccacgctca
    58381 ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact gaacagcgtc
    58441 gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta gagaaacgcg
    58501 aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg gaccgacgcc
    58561 cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt gaataactgt
    58621 cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat ctgtttgaaa
    58681 attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag atccttgaga
    58741 accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt gcgaccagcc
    58801 tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag ggcgatggtc
    58861 cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt gtggcgcgcg
    58921 tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat ggttacgtgg
    58981 tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt ctggaaggtg
    59041 ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg ccgacgcgcc
    59101 tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg ttgttgcagg
    59161 taatgcctgc gcaaaatacc cagcaggacg actttgacca cctggcgacg ctaaccgaaa
    59221 ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg cgtttgtatc
    59281 acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc acctgctcgc
    59341 gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat agcatcctgg
    59401 cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat ctgttcaatg
    59461 cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgcaa gttcattaat
    59521 gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct gatgtgctgc
    59581 gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag cgtcgtatcc
    59641 gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct accgtcagat
    59701 cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg agtaattttt
    59761 ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct tttctgtctg
    59821 agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc cgctccgcct
    59881 gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca atacaggtca
    59941 tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt ttaacagaac
    60001 caggggcaat taacacattt tcggctaata ctttttccga tacctgttcc tgaagatgtt
    60061 gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc ataaacccgg
    60121 catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt tcgagcatgt
    60181 agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata tgactgttca
    60241 cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc accaacacca
    60301 ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc ccattacgcc
    60361 agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc gccagcgcgc
    60421 cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac ccccaggtgg
    60481 cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa cgcaacggcc
    60541 ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag atcaattgca
    60601 aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc gcgctcaggc
    60661 ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac ggcagcaacc
    60721 ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac tgtgtacgcg
    60781 cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac cagtcggttg
    60841 caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca ccccacagta
    60901 aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata agtacgttat
    60961 gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata cgaaggcttt
    61021 gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca aaatcccaaa
    61081 tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc caaccgctca
    61141 tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc tgtttaaaag
    61201 agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg ccataaacaa
    61261 tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc agcccctgaa
    61321 gtaatccggt agcaatcatc ccccagcgtg ttgccggtga aagttcaacg ttatccatgt
    61381 tggttatcca gaatcaaaag gtgggttaat tatcgcatcc gggcagtagt attttgcttt
    61441 tttcagaaaa taatcaaaaa aagttagcgt ggtgaatcga tactttaccg gttgaatttg
    61501 catcaatttc attcaggaat gcgattccac tcacaatatt cccgccatat aaaccaagat
    61561 ttaacctttt gagaacattt tccacaccta aaatgctatt tctgcgataa tagcaaccgt
    61621 ttcgtgacag gaatcacgga gttttttgtc aaatatgaat ttctccagat acgtaaatct
    61681 atgagccttg tcgcggttaa cacccccaaa aagactttac tattcaggca atacatattg
    61741 gctaaggagc agtgaaatgc gcgttaacaa tggtttgacc ccgcaagaac tcgaggctta
    61801 tggtatcagt gacgtacatg atatcgttta caacccaagc tacgacctgc tgtatcagga
    61861 agagctcgat ccgagcctga caggttatga gcgcggggtg ttaactaatc tgggtgccgt
    61921 tgccgtcgat accgggatct tcaccggtcg ttcaccaaaa gataagtata tcgtccgtga
    61981 cgataccact cgcgatactt tctggtgggc agacaaaggc aaaggtaaga acgacaacaa
    62041 acctctctct ccggaaacct ggcagcatct gaaaggcctg gtgaccaggc agctttccgg
    62101 caaacgtctg ttcgttgtcg acgctttctg tggtgcgaac ccggatactc gtctttccgt
    62161 ccgtttcatc accgaagtgg cctggcaggc gcattttgtc aaaaacatgt ttattcgccc
    62221 gagcgatgaa gagctggcag gtttcaaacc agactttatc gttatgaacg gcgcgaagtg
    62281 cactaacccg cagtggaaag aacagggtct gaactccgaa aacttcgtgg cgtttaacct
    62341 gaccgagcgc atgcagctga ttggcggcac ctggtacggc ggcgaaatga agaaagggat
    62401 gttctcgatg atgaactacc tgctgccgct gaaaggtatc gcttctatgc actgctccgc
    62461 caacgttggc gagaaaggcg atgttgcggt gttcttcggc ctttccggca ccggtaaaac
    62521 caccctttcc accgacccga aacgccgcct gattggcgat gacgaacacg gctgggacga
    62581 tgacggcgtg tttaatttcg aaggcggctg ctacgcaaaa actatcaagc tgtcgaaaga
    62641 agcggaacct gaaatctaca acgctatccg tcgtgatgcg ctgctggaaa acgtcaccgt
    62701 gcgtgaagat ggcactatcg actttgatga tggttcaaaa accgagaaca cccgcgtttc
    62761 ttatccaatc tatcacatcg ataacatcgt taagccggtt tccaaagcgg gccacgcgac
    62821 taaggttatc ttcctgactg ctgatgcttt cggcgtgttg ccgccggttt ctcgcctgac
    62881 tgccgatcaa acccagtatc acttcctctc tggcttcacc gccaaactgg ccggtactga
    62941 gcgtggcatc accgaaccga cgccaacctt ctccgcttgc ttcggcgcgg cgttcctgtc
    63001 gctgcacccg actcagtacg cagaagtgct ggtgaaacgt atgcaggcgg cgggcgcgca
    63061 ggcttatctg gttaacactg gctggaacgg cactggcaaa cgtatctcga ttaaagatac
    63121 ccgcgccatt atcgacgcca tcctcaacgg ttcactggat aatgcagaaa ccttcactct
    63181 gccgatgttt aacctggcga tcccaaccga actgccgggc gtagacacga agattctcga
    63241 tccgcgtaac acctacgctt ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa
    63301 actgtttatc gacaacttcg ataaatacac cgacacccct gcgggtgccg cgctggtagc
    63361 ggctggtccg aaactgtaat gatttgaagc tggagaatat ctatccagta tcttatagaa
    63421 agcaaaacgg gaggcacctt cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg
    63481 cccgcgttac cggcactggc agccaggcgc gaatggaaag cccgccccgc tcgctggtgc
    63541 caagctccag catcccgtta tggttatcca cgatacgctg cacaattgcc agccctaatc
    63601 ccgtgccgct aatggtgcgc gcactgtcgc cgcggacaaa cggctggaac aggtgcttac
    63661 gttgttccgg cgcaattccc ggaccgtcat cttccacctg gaaccaggcg cgattcggct
    63721 ccgttccgct gctgactttg atccagccat tgccataacg ggcggcgttg accaccatat
    63781 tcgccaccgc gcgtttgatc gacagcgggt gcattttcac ttcaatgctg ccggggtaaa
    63841 gcgcggtttc aatttcccgc tcatagccac tttcggcagc aatcacctca ccgagcactg
    63901 cattaagatc cgccatttcc atcggcatct cctgcccggt gcgcaggtag tcgataaact
    63961 gctcaatgat ggcgttgcac tcttcgatat ctttattgat cgattctgcc agatagccat
    64021 cctgctcgct catcatctca gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt
    64081 gacttacccc cgccatcagc agcgtgcggt catccgccag ttgcttaaca ccagccgcca
    64141 tatggttaaa ggcacgggta acggaacgca cctccgaagc gccatattca cgcagcggcg
    64201 gcggaataat ccctttacca acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt
    64261 tctggatacg aataaacagc cacgccccgc ctatcgccaa tagcataatc gccagcgtat
    64321 agcggaacag cggagagaaa tcgccctgat gaatttcggt cagcggcacg cgtacccaga
    64381 tattgggcga cagccaggtt ttcagccaga cgacaggcga acttttgttg acctcaacgc
    64441 gcacttccgt cgggccgccc agttgctgtg ccatctgatg gcttaagaat tcatagtgtt
    64501 gcgcccaacg cagacctgcc tcttcggcag cctcgttgga gtagagagag atccccagct
    64561 cacggtagat ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt
    64621 gcagtttatc ggtcatcaac atacgcactt cgtacgcgag gactttatta aactgctgga
    64681 ggctcggcaa aatcgcgaag ttcagcacca ccagataagt cgtcaccagg ctggcgaaca
    64741 gcaaggtgac gatgagcaat aacgtacggg caaatgaact tcgtggcgag aagcgcaatc
    64801 gcctcatgct ttagagccgt ccggtacaaa gacgtagccc aggccccaaa cggtctgaat
    64861 gtaacgcgga tgcgctggat cttcttctac catgcggcgc agacgcgaaa tctgcacgtc
    64921 gatggagcgt tccattgcgg aatattcacg accacgggca aggttcatca gcttatcgcg
    64981 ggagagcggc tcacgcggat ggctgaccag tgccttcagt accgcaaact caccgctggt
    65041 gagcggcatc ggctcatctt cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt
    65101 accgaaagca attaccgcct cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg
    65161 acgcagcacc gcacgaatac gggccagcag ttcacgcggg ttaaacggtt ttggaatgta
    65221 gtcgtcagcg ccaatctcca ggcctacgat acggtccact tcttcccctt tcgccgtcac
    65281 cataatgatc ggcatcgggt tgctctgact acgaagacgt cggcaaatcg acaagccatc
    65341 ttcaccaggt aacattaaat ccagtaccat aagatggaaa gattcacgag tcagcaggcg
    65401 atccatctgt tctgcattag cgacgcttcg aacctggaag ccttgttcgg tgagataacg
    65461 ttccagcagc gcacgcaggc gcatgtcgtc atcgaccacc agaatcttgt agttctcttg
    65521 cattgtttgt actcccaaag gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc
    65581 acgttcgtca ccagctaaat ctggtatgaa tttcagccta aattgttaca aagcatatta
    65641 aacagcagct taagtataca atttattcgg cgaaacatta ttgattctgt tgatatgatc
    65701 acgttatacc caatgtgcgc attatcaaac agacaaaggg aatcaacgag atgaaaacgc
    65761 ccctggttac ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg
    65821 aagaacgccc ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg
    65881 aaaatgctga ctatcagtat aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct
    65941 atctcactaa atgcctggaa aatctcaaaa ttgtcgatta ctcccctcag caggaaggca
    66001 aagtcttttt tggcgcgtgg gtggagattg aaaacgacga tggcgtgact caccgtttcc
    66061 gtattgtcgg ctacgatgaa atttttggcc gtaaagatta catctctatc gattccccga
    66121 tggcccgcgc attgctgaaa aaagaagtcg gcgatctggc ggtggtgaat acccctgccg
    66181 gggaagcgaa ctggtatgtt aatgctatcg agtacgtgaa accgtaagga agagtcttaa
    66241 cctcctgccg atggctggca tttttgccag ccagtccgta taactatccc ctgatttttg
    66301 atccgaaaag atgaactcaa accatgatga atgattcgtt ctgccgcatt attgcgggtg
    66361 aaattcaggc gcgcccggaa caggttgacg ctgccgttcg cctgcttgat gaagggaata
    66421 ccgtgccgtt tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat gacacgcagc
    66481 tgcgtaatct ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga
    66541 tcctcaaatc catttccgag caaggcaaac tcaccgatga tctggcgaag gccatcaacg
    66601 ccaccctaag caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct aaacgccgca
    66661 cccgcgggca aatcgccatt gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg
    66721 atccgtcaca cacgccagaa gtcgccgctg cacaatatgt tgatgccgat aaaggcgtgg
    66781 cagataccaa agccgcgctg gatggcgcgc gctatatcct gatggaacgg tttgccgaag
    66841 atgccgcgct gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat ttggtttcta
    66901 cggtggtgag cggtaaagaa gaggaagggg cgaaattccg cgactatttc gatcatcacg
    66961 aaccgttgtc cacggtgcct tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag
    67021 gcgtactcca gctttcgctg aatgccgatc cgcagttcga tgagccgccc aaagagagct
    67081 attgcgagca aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc ccggcggata
    67141 gctggcgcaa aagcgtagtg agctggacct ggcgcatcaa ggtgctgatg catctggaaa
    67201 ccgaactgat gggtaccgtg cgcgaacgcg cggaagatga agcaatcaac gtctttgccc
    67261 gtaacctgca cgatctgctg atggcggccc ctgccggact gcgtgcaacg atgggcctcg
    67321 atccgggtct gcgtaccggg gtaaaagtgg cggtggtcga tgccactggc aaactggtgg
    67381 cgaccgatac catttacccg cacaccggac aagccgcaaa agcagcgatg accgttgctg
    67441 ccttgtgtga aaaacataac gttgaactgg tggcgatcgg caacggtaca gcttcccgcg
    67501 aaactgaacg tttctatctc gacgtgcaga agcagtaccc gaaagtgacc gcacagaaag
    67561 tgatcgtcag cgaagctggc gcgtcggttt actcagcttc cgagctggca gcgcaggagt
    67621 tcccggatct cgacgtttcg ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc
    67681 cgctggcgga gctggtgaaa atcgatccga aatctatcgg cgtaggtcag tatcagcatg
    67741 acgtcagcca gacgcaactg gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg
    67801 gcgttggcgt cgatctcaac accgcttctg ttccgctatt aacccgcgtg gcgggcctga
    67861 cgcgcatgat ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag ttccagaacc
    67921 gtcagcaact gctgaaagtc agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct
    67981 tcctgcgcat taaccacggt gataacccgc tggacgcctc taccgttcat ccggaagcct
    68041 atccggtggt ggaacgcatt ctggcagcaa cacagcaggc actgaaagat ctgatgggta
    68101 acagcagcga actgcgtaac ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc
    68161 cgacagtaac tgacatcatc aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat
    68221 ttaaaaccgc tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga
    68281 tcctcgaagg cgcagtgacc aacgtcacca actttggcgc gtttgtcgat attggcgtgc
    68341 atcaggacgg cctggttcac atctcttcat tgtcgaacaa gtttgtggaa gatccgcata
    68401 ccgtggtgaa agcgggcgac attgtgaagg tgaaagtgct ggaagtggat cttcagcgta
    68461 aacgtatcgc cctgactatg cgtctggacg agcagcctgg cgaaaccaac tctcgtcgcg
    68521 gcggcggtaa tgaacgcccg caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag
    68581 cgcagcctgc cggtaatagc gcgatgatgg atgcgctggc ggcagcgatg ggtaaaaaac
    68641 gttaaacgcc cggactggcc cctaaacctg cgcgataagg cgttcacgcc gcatctggca
    68701 acgaacgctt tgcctgacat aaaagtgccg gagaatatct ccggcacttt tattccacag
    68761 ccaaactcac aatatactcc gttaatattt atcacttcat taacaactga aaccttaatt
    68821 aaacattagc cagtccgggt aactcactat tcgaattata ttttcgctgc gatataacct
    68881 tgagccacat caacattgag tcagattatt attcaaacca acattcgtac acattttaag
    68941 tattgctgat agaaaccatt ctcattatca ttgtgttgtt gattatttaa tctctccttt
    69001 gttggcaaat catctggtct catgtcgcgg tcaaacgccc catgaggtag ttatccagtt
    69061 aatgagaaac aagtaggcac ctatgcaata cactccagat actgcgtgga aaatcactgg
    69121 cttttcccgt gaaatcagcc cggcatatcg ccaaaaactg ctttctctag gcatgttacc
    69181 tggctcctct tttgatgtgg tgcgcgtcgc tccactcggc gaccccattc atatcgaaac
    69241 ccgtcgtgtg agcctggtat tacgcaaaaa agatctggcc ttattagaag tggaagcggt
    69301 ttcctgttaa tacggtgata acaacaatga aaaaattaac cattggctta attggtaatc
    69361 caaattctgg caagacaacc ttatttaacc agctcactgg cgcacgtcag cgtgtaggta
    69421 actgggctgg cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc gatcatcagg
    69481 tcacgctggt ggacctgccc ggcacctatt ctctgaccac catctcatcg cagacctcgc
    69541 tcgatgagca aatcgcctgt cactacattt tgagtggcga cgccgacctg ctgattaacg
    69601 tggtggatgc gtctaacctt gagcgtaacc tgtacctgac gctgcaactg ctggaactcg
    69661 gcattccctg catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa aatattcgta
    69721 ttgaaattga tgctctatcg gcacgtcttg gctgcccggt gatcccgctg gtttcaaccc
    69781 gtggtcgcgg tattgaagcg ctcaagctgg cgattgatcg ctataaagct aacgagaatg
    69841 tggaactggt gcattacgca cagccgctac tcaacgaagc agattcactg gcaaaagtga
    69901 tgccttccga catcccgctg aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg
    69961 atatctacag ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc accctcgccc
    70021 gtctgcgtaa tgaaatggat gatccggcgc tgcacattgc cgatgcgcgt taccagtgca
    70081 ttgctgccat ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc cgtttcacca
    70141 ctgcggtaga taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc ctctttgtga
    70201 tgtacctgat gttcctgctg gctatcaaca tcggcggggc gttacagccg ctgtttgacg
    70261 tcggctccgt ggcgctattt gtgcatggta ttcaatggat tggctacacg ctccacttcc
    70321 cggactggct gactatcttc ctcgcccagg gcctgggtgg cggtattaac accgtgctgc
    70381 cactggtgcc gcagatcggc atgatgtacc tgttcctctc cttccttgag gactccggct
    70441 atatggcgcg tgcagcgttt gtgatggacc gcctgatgca ggcgctgggc ttgccgggga
    70501 aatcctttgt gccgctgatc gtcggtttcg gttgtaacgt accgtcggta atgggcgcac
    70561 gtacgcttga tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg tttatgtcct
    70621 gcggcgcgcg tctggctatc ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg
    70681 ccctggcggt tttctcgctg tatatgctcg gtatcgtgat ggcggtgctg actggcctga
    70741 tgctcaagta caccatcatg cgcggtgaag caacgccgtt tgtcatggag ctgccggtct
    70801 accatgtacc acacgttaaa agcctgatta tccagacctg gcagcgtctg aagggcttcg
    70861 ttctgcgtgc tggtaaagtg attatcatcg tcagcatttt cctgagcgct ttcaacagct
    70921 tctcgttgag cgggaaaatc gtcgataaca tcaacgactc ggcgctggct tccgtcagcc
    70981 gggtgataac ccctgtcttc aagccgattg gcgtacatga agataactgg caggcaacgg
    71041 ttggcctgtt tacgggcgca atggcaaaag aagtggtggt gggtacgctc aacaccctct
    71101 acaccgcaga aaatattcag gacgaagagt tcaatccggc ggaattcaac ctcggtgaag
    71161 agctgttcag tgcggtagat gaaacctggc agagcctgaa agacaccttc agccttagcg
    71221 tactgatgaa ccccattgaa gctagcaaag gcgacggtga aatgggtacc ggggcgatgg
    71281 gcgtgatgga tcagaaattc ggtagcgcag ctgccgcata cagctacctg attttcgtcc
    71341 tgctgtatgt accatgtatc tcggtgatgg gagccatcgc ccgtgaatca agccgtggct
    71401 ggatgggctt ctccatcctg tgggggctga atatcgctta ctcactggca acattgttct
    71461 atcaggtcgc cagctacagt cagcacccaa cttacagcct ggtgtgcatt ctggcggtaa
    71521 tcctgtttaa catcgtagtt atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg
    71581 aactgctggc aacccgcaag tcggtaagca gttgctgcgc agccagcacc accggtgatt
    71641 gccattaatg gcttcactta ttcaggtacg tgatttgctg gcgttacggt gccgtatgga
    71701 agcgacccag ataagccaga cattgaacac tccgcagcca atgattaacg ccatgctgca
    71761 acagctggaa agcatgggca aagccgtgcg gattcaggaa gaacctgacg gctgcctctc
    71821 tggcagttgt aaaagctgcc cggaaggcaa agcctgtctg cgcgagtggt gggcgctgcg
    71881 ttaaccatac tccatcgcct gttttgaaaa gcccggtatg cgtttgcatc cgggcttttt
    71941 tgcgtgcggc tttccataaa aatgcaattc ttgcagcgca acgtaagttc ctctgaaagc
    72001 gtctcgcagg gatgaaaact cgctaatgca cagatgtgga gtggcgcgta gagtcgcggc
    72061 attcaaacaa caggtgaagg aacgccatga gtaaaaagca gagttccacc ccacatgatg
    72121 cgctgttcaa actcttttta cgccaaccgg agacggcacg cgattttctt gcgtttcatt
    72181 taccggcacc cattcacgcg ctctgtgata tgaaaaccct caagctggag tcgagcagct
    72241 ttattgatga cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac
    72301 aaggaccagg atacatctat tgtctgattg aacatcaaag cacctcaaac aaactgatcg
    72361 catttcgcat gatgcgttac gctattgccg caatgcaaaa tcaccttgat gctggataca
    72421 aaacgttgcc gatggtggtg ccattgttgt tttaccacgg tattgaaagc ccctatccct
    72481 attcgctgtg ttggctggat tgtttcgccg atcccaaact ggcaaggcag ctttatgcct
    72541 ccgcatttcc gctgattgat gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac
    72601 gtatggcgct gctggagtta attcaaaaac atattcgtca acgcgatctg atggggctgg
    72661 tagagcaaat ggcctgctta ttaagtagtg gatacgctaa tgacagacaa atcaaagggc
    72721 tgtttaatta catactgcaa actggcgacg ctgtacgttt taacgatttt atcgacggcg
    72781 ttgccgaacg ttcaccgaaa cacaaggaga gtttaatgac tattgcggaa agattgcggc
    72841 aggaggggga acaatccaaa gccctgcata tagccaaaat aatgcttgaa tccggagtcc
    72901 ctcttgcaga catcatgcga tttaccgggc tgtcagaaga agagttggct gcggcgagtc
    72961 agtagatact ctaccctgct taagtgctac tttgtgctat cttgcatgta acacaaagta
    73021 gcacttaggg aactgatatg cagacagtga aattacggca acaaggagga gccatgatcg
    73081 ttactatccc acgagatctt gccatcgatc ttggctggtc accgggcaca gagcttaccg
    73141 tggagaaaaa aggcgatagc gttaatttgc gcgccacaga gcacaaaccg cgtgggcgtc
    73201 ttaccgtcgc gcagttgttg agccaaatcg atgaaagcga aattacggag ctaaatcagt
    73261 ccacagaagg atgggctgaa gggaaaaaag gtaatgaagc atggtaaagg cacggacgcc
    73321 acatcgtggt gagatctggt attttaaccc tgatccggtt gccgggcatg aacttcaggg
    73381 gccacattat tgcattgtgg taacggacaa aaaactcaac aatgttttaa aagttgctat
    73441 gtgctgcccg atttcaacag gggcaaatgc agcacgttcc acaggggtga cggtgaacgt
    73501 cctcccctgt gatacgcaaa ccggtaacct gcatggcgtt gtactttgtc accagctaaa
    73561 agccgtcgat cttattgccc gtggcgctaa atttcatacc gttgccgatg aaaaattgat
    73621 tagtgaagtt atcagtaaac tggtgaattt aatcgaccca caataattag ctctcactta
    73681 ccaccaacac cctctgcttc aacgccacca gcaggtgaca aaactcgacc ggatgcgaaa
    73741 taaacggcgc atgggccgct ttggcgaaga tatatgattc gctgtgaggc caaagtttat
    73801 ccagcatcgg caccactttg cgcggcacca gaccgtcgag atagccatac aatcgcaaaa
    73861 acggcatgga cacgttttgc agcggctgac ggagatcgac cgttttcagg atttccagcc
    73921 cgccattaag cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcaacgccc
    73981 gcgcatcctg gcgcgccgtt tcagtcccca tggtttgtaa cgccaggaac cgctccactg
    74041 tacgctgaaa atcatcactg agttgctgct gaaatcccgc cagcacgtcc ggttttatcc
    74101 ccggccactc gtcacgagca ctaaaataag gtgacgacgc cacggtgacc agcgcctgaa
    74161 cacgctcggg atgggttaac gcaatctggc ttgccaccag cccgcccaga ctccagccta
    74221 accaaatggc tttatcaggt gcctgttgca gcacggcttc ggccatatca gcaagtgaca
    74281 gcgcaccaaa tccccggcta cgcccgaagc cgggcaggtc aacaaggtgc agcgtaaaat
    74341 gcgagctaag ttcctcgtca atgcaacgcc acacttcggc attcagtccc catccgtgca
    74401 gcagcacaag atgaacattc ccctgacctt tggtctgcca ccagatgtta ttcatccgct
    74461 attgttctct tttgacttac aaggatgaac atatgctaac agtaccggga ttatgctggc
    74521 tatgccgaat gccactggcg ttaggtcatt gggggatttg ttcggtctgt tcacgcgcca
    74581 cccgcacaga taaaacgtta tgcccacaat gtggattacc cgccacacac tcccatcttc
    74641 cctgcggtcg ctgcctgcaa aaaccgccgc cctggcaaag actggtcacg gttgccgact
    74701 atgcgccgcc gttaagtccg cttatccacc agcttaaatt ttcccggcgc agtgaaatcg
    74761 ccagcgccct gtcacgtctg ttactattgg aagtcttaca cgctcgtcgc accaccgggt
    74821 tgcaattgcc ggatcgcatc atcagcgttc cgttatggca gcggcgtcac tggcgtcggg
    74881 gatttaatca gagcgatttg ctgtgtcagc cgttatcacg ctggttgcac tgccaatggg
    74941 atagcgaagc cgtcacacgt acacgggcca ctgcgaccca gcattttctc agtgcccggc
    75001 tgcgcaagcg caacctgaaa aatgcctttc gtcttgaatt gcccgtgcaa ggtcgccata
    75061 tggtgattgt ggatgatgtc gttaccaccg gaagtaccgt cgcagagatt gcgcagttgc
    75121 ttttacgcaa tggtgcggcg actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc
    75181 tcgatgatgg gcgtattata accaactaaa atagtcaact attaggccat tactatgatc
    75241 cgtatttccg atgctgcaca agcgcacttt gccaaactgc tggcaaatca ggaagaaggg
    75301 acacaaatcc gcgtatttgt gattaaccct ggcacgccta acgctgaatg tggtgtttct
    75361 tattgtccgc cggatgccgt ggaagccacc gacactgccc tgaaatttga cctgctgacc
    75421 gcgtatgttg atgagttaag cgcaccatac ctggaagatg cagagatcga ctttgttacc
    75481 gaccagttgg gttctcagtt aacgctgaaa gccccgaacg ccaaaatgcg taaagtggca
    75541 gacgatgcac cgctgatgga gcgcgtggag tatatgctgc agtcgcagat caacccacag
    75601 cttgctggtc acggtggtcg cgtttcgctg atggaaatca ccgaagacgg ttacgccatt
    75661 ctgcaatttg gcggcggctg taacggttgt tccatggtcg atgtgacgct gaaagaaggg
    75721 atcgagaagc agctgctgaa cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa
    75781 caccagcgcg gcgaacactc ctactactaa gttatcccct cttttgtaga ttgcccgatg
    75841 cgacgctaaa gcgtcatttc gggcctacgt tgaacatgtg ccagacgttg gcacagcatt
    75901 gctttggcaa atccctccct tcgtttcccc gcgataatat gaccaacctc tcataattta
    75961 aatttacccc gctctggtga ttctcaaacg ccagatgtta cccgtatcat tcacatgggt
    76021 accaaacata ctcctgacat ctgactacaa taattagttt tagtgggtat cagtcgtggt
    76081 gccgcaatat ctctgttccc gattgggata attagagttt gtcgtcagaa aattgacgtt
    76141 acccataaca aatgaaaggc caggtaaatc atgccattag tcattgttgc tatcggtgta
    76201 atcttgttgt tgctcctgat gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc
    76261 gtggcgcttg ctgttggatt aatgcaagga atgccgctgg ataaagttat tggctccatc
    76321 aaagccggtg tcggcgggac gctcggtagc cttgccctga tcatgggttt tggcgcaatg
    76381 ctggcaaact gcggtggcgc acaacgtatc gccaccacgc tgattgccaa atttggtaaa
    76441 aagcacatcc agtgggcggt ggtgttaacc ggttttaccg ttggttttgc cctgttctat
    76501 gaagtgggct tcgtgctgat gctgccgctg gtgtttacca tcgcggcttc cgcgaatatt
    76561 ccattgctgt atgttggtgt accaatggcg gctgcactgt ctgtgaccca cggcttcctg
    76621 ccaccgcatc cgggcccgac tgcgattgcc accattttca atgccgatat gggtaaaacc
    76681 ctgctgtacg gtactattct ggcaatcccg accgtgattc tcgccggtcc ggtttacgct
    76741 cgcgtgttga aaggtatcga taagccaatc ccggaaggtc tctacagcgc gaaaaccttc
    76801 agcgaagaag agatgccgag ctttggcgtc agcgtctgga cctctctggt gccggttgtg
    76861 ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc tttcctgccg
    76921 gtagcggagt tcctcggtga cccggtaatg gcaacgctga ttgccgtgct gattgcgatg
    76981 ttcacctttg gtctgaaccg tggtcgttca atggatcaga ttaacgacac gctggtttct
    77041 tccatcaaaa tcattgcgat gatgctgttg atcatcggtg gtggcggtgc gttcaagcag
    77101 gtgctggtag acagcggcgt ggacaaatac attgcttcca tgatgcacga aaccaacatt
    77161 tctccgctgc tgatggcctg gtcgattgct gccgtactgc gtatcgcgct gggttccgca
    77221 accgttgcgg caatcactgc gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt
    77281 agcccagagc tgatggttat tgcggtgggt tccggtagtg tgattttctc tcatgtgaac
    77341 gatccgggct tctggctgtt caaagagtac tttaacctga ctatcggcga gaccatcaaa
    77401 tcctggtcga tgctggaaac cattatctcg gtgtgcggtc tggtaggctg tctgctgctg
    77461 aatatggtga tttgataaca ctgactgccg gatgcatcgc atccgacatc aaccgcactc
    77521 tacttcttct tcgctgcagc tctgcgccgt ctgtccaaat ccttcagcaa cttgttcacg
    77581 ccatcatcgg caaacatcga ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac
    77641 tggtaactgg tgccaggaat attcaccggt tcggccatat ccagccagtc ttccggctgt
    77701 agtcctaaca gagcactgtt actgtcggca atgtagcgct gcaaaccacg gttcagcgtc
    77761 ggcgtcatcg acatcaacga tgccttatgc ccggcacgtt ttggcagaca accatattta
    77821 tgcagtgcat ccagcagccc ttgcttcgcc agttcgcgat cctgatacag accgcgcagt
    77881 accacttcat ccggatacag ccccagggtt ttgcccagcg ttagatcccc gctctcccag
    77941 taaccacgca gcgttggcag gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac
    78001 gctttcggtg cacggaacgt cttctcgtgg tcgttttcga aatagagcac tttgtaagag
    78061 tacacaccgc tgctgcgcag cttaccgaca atctctaccg gtacggtacc gagatcttca
    78121 ccaatcacca tacagcgatg acgtttactt tcgagtgcca gaatcgagag cagatcatcc
    78181 accggatagt gaacatacgc gccctgatct gccgtctcgc catacggtat ccaccacaaa
    78241 cgcagcatcg acatcacatg gtcaattcgt aatgcgccgc agttttgcat attggcacgc
    78301 aacagctcga taaacggttc ataggcacgc gcggtgatga tatgcgggtc cattggcggt
    78361 aatccccagt tctgccccaa cgggccgagg atatccggcg gcgcgccaac cgatgctttc
    78421 aggcaatata gttcacggtc acaccaggtt tccgccccac cttccgctac gccaaccgcc
    78481 agatcacgat acaagccaat cggcatttca tagccctggc ttatctccca gcaggcggca
    78541 aactggctgt aagccagcca ctgcaaccag agataaaaat cgacgtcatc acgatgttct
    78601 tcgcagaact gacgcacttc tggtgaatcc acgttcttat acatctctgg ccatgcaggc
    78661 cagccccagc gcatttcgtc ctctttcact tgctgggcat gtagcgcatc aaaggctgcc
    78721 tgccagaaca ggctgtcgcc ctgctctgca acaaactggc gaaacgcggc catctgctca
    78781 tcatcacgtt gcgcgaaacc tttccacgcc attcgtaatg ctgtcatttt tagggcggta
    78841 accgtggagt aatcgaccca gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc
    78901 ggcaactgcc accaggcctg agcctcttcg ctaagatgga aatcttcaac ggcgttaacg
    78961 tcgatataaa tcacattcag ccaacggcga gaagacgggc tgtatgggct ggcgctctcc
    79021 ggatttgccg gatagagcgc atgaatcggg ttcaggccaa tgaacgaccc gccacgtttt
    79081 gccacatcca ccagcatcgc tttgagatcg ccaaaatccc caatacccca gtttttttcc
    79141 gatcgcagcg tataaagctg aacgcaggca ccccacagct tttgtttatt cagcaaagcc
    79201 tgcggttcgt aacaacgttt cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc
    79261 tgggtgagtg ttagcgtgtg ataaccttcc ggcagcttcg tcggtagatt gaacgctttg
    79321 ccccccgtta catggccttt gtactgcgtt ccttcttcgg tggtcagctg ccagctatat
    79381 tcgccgctgc cctccaccac catcggcatt tttttgccgc tggtataaac catgacattc
    79441 gggactggcg ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc
    79501 cgtttggttt cggcgctaat cgactgcggt ttaccgtggg cattgatgta attggggcta
    79561 atccccgccg ccagcgcggc attatccaga cgtttgcttt ccatcgagct tccttagcgt
    79621 tttgcctgcc agatacgagc ctgataatcg cgaatagagc gatccgagct aaacataccg
    79681 cagcgggcgg tattgaggat cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca
    79741 tccacctgct tttgtgcctc aacataggct gcgaaatccg ccatcaccag atacggatcg
    79801 ccgccctgtt tgccgatact gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg
    79861 tatttaccgc tttccagctc tttcaatact gcgtccagca ccttatcttt cttccgccat
    79921 ttcaccgggt cgtagccttt ggccagaata gccttcactt gttccacggt atgaccaaaa
    79981 ataaagatat tttcttcacc gactttctcg gcgatttcaa cgttcgcacc atccagcgta
    80041 ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc cggtaccgga agcttcttta
    80101 cctgcagtcg aaatttgttc ggagatatcc gccgccggga tcagtttttc cgccgccgaa
    80161 acgcaataat ccggcaggaa caccaccttc aacttatcgc caaccagtgg atcgttgttg
    80221 atcacgtcag ccactttgtt gatcgcaaag ataatattct tcgccaggta gtagcccggt
    80281 gccgctttcg cgccgaagag gaagacgcgc ggtacgcgat cagcctgcag gttttcacga
    80341 atttctttgt acaacgccag aatatgcagc agattcaggt gctggcgttt gtactcatgc
    80401 agacgtttga tctgaatatc gaaaatcgcc tgtggattga tctcaatacc ggtacgaact
    80461 ttcacaaact ccgccagacg gactttattc gcctgcttga tctcgcgata ttgctgacgg
    80521 aatttcgcat catcagcgaa tttttccaga ttgatcagct gatcgagatc gttagcccac
    80581 tctttttgca gtgatttatc caacagagcc gccagtgcag ggttgcactg tttgatccag
    80641 cgacgtgggg taataccgtt ggtgacgtta tggaatttgt tcggccatag ctggtgatat
    80701 tccgggaaca gatctttcac caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg
    80761 aaaccgccaa ccacacacag gttcgccata tgcacttgtt tatcgtgcac caccgccagt
    80821 ttggcccaca ctttttcatc gcccggccag gttttctcca ccagcgtttt aaagcgagta
    80881 ttaatttcgt taataatctg catatggcgc ggcagtaagc ctttcaccag tttcacatcc
    80941 cagcgttcca gcgcttctgg catcagggta tggttggtgt aagcgaaagt tttgctggtg
    81001 atggcccagg cgtcatccca gctcatctgg tgctcatcgg tcagcacgcg cagcagttct
    81061 ggaatcgcga tagttggatg ggtatcgttc agctgaataa cttcgtaatc cgccagttcg
    81121 tgcagtttac gccccgccag atgatggcga cgcaaaatat ccgctaccga acaggcacac
    81181 tggaagtatt gctgcatcag gcgcagcttt ttaccggcag tatggttgtc gtttggatag
    81241 agaactttgg tcagtttttc cgcattgatg ccttgctgtt cggcgcgcag gaaatcaccg
    81301 tcgttaaatt tagttagatc aaacggatgc gcatgcgtcg cctgccacag acgcagcggc
    81361 tgcgccacgc cattacgata gccgacaacg gggagatccc acgcttgacc ggtaatggta
    81421 aactccggct cccagcgtcc gtctttcatc actttaccgc caatccctac ctgcacatcc
    81481 agtgcttcgt tgtggcggaa ccacgggtag ttactgcgat gccagtcatc cggcgcttca
    81541 acctgtttgc catcgacaaa agactggcgg aacaaaccat attgatagtt cagaccgtaa
    81601 cccgtcgcag actgaccgac agttgccatt gagtcgagga agcacgccgc cagacgtccc
    81661 agaccaccgt tacccagcgc cgggtcgatc tcttcttcca gcaggtcagt caggttgatg
    81721 tcataagcct tcaacgaatc ctgtacatcc tgataccagc cgagattcaa caggttgttg
    81781 cccgtcagac gaccaatcaa aaactccatt gagatgtagt taacatgtcg ctgattcgcc
    81841 accggtttgg cgaatggctg agcacgcagc atttcggcca gtgcttcact cactgccagc
    81901 caccactggc gaggagtcat ttcagccgca gaatttaagc cataacgctg ccactgacgt
    81961 gaaagcgctt cctgaaattg cttatcgtta aaaataggtt gtgacatagg agttccactt
    82021 ttcttagatt ttcaacacaa cgttatcgct agtttgccag gctcgatgtt gaccttcctc
    82081 atcctgcggg ggattaggca gggaggagtt gcggggatga gcaaggaaat gtgatctcga
    82141 ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat caatgccata cagcgcagaa
    82201 aatctgtatc taagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc
    82261 tataaattaa gtaaaggagt gaaacagttt cacaagtaaa atatccagtg tgctccatct
    82321 cattcttaat agatttatta agatcatctt tttagatggc actttcatca ggaatgaaga
    82381 agaaaccctt gcttaaatga atctgat
//
LOCUS       DADLXX010000003        76412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLXX010000003 DADLXX010000000
VERSION     DADLXX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.003"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..545
                     /locus_tag="JNX27_000176"
     CDS             <1..545
                     /locus_tag="JNX27_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HAY7881521.1"
                     /translation="FPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKEN
                     SLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVI
                     EQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASY
                     TQLEIKHSHPKNRASGKRVS"
     gene            1088..1580
                     /locus_tag="JNX27_000177"
                     /pseudo
     CDS             1088..1580
                     /locus_tag="JNX27_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1683..2186
                     /locus_tag="JNX27_000178"
     CDS             1683..2186
                     /locus_tag="JNX27_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY7881522.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNX27_000179"
     CDS             complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNX27_000179"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7881523.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNX27_000180"
     CDS             complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNX27_000180"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7881524.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNX27_000181"
     CDS             complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNX27_000181"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7881525.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNX27_000182"
     CDS             complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNX27_000182"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7881526.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNX27_000183"
     CDS             complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNX27_000183"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7881527.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNX27_000184"
     CDS             complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNX27_000184"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7881528.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNX27_000185"
     CDS             complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNX27_000185"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7881529.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNX27_000186"
     CDS             complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNX27_000186"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7881530.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNX27_000187"
     CDS             complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNX27_000187"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7881531.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNX27_000188"
     CDS             complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNX27_000188"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7881532.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNX27_000189"
     CDS             complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNX27_000189"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7881533.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16156..16818)
                     /locus_tag="JNX27_000190"
     CDS             complement(16156..16818)
                     /locus_tag="JNX27_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7881534.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNX27_000191"
     CDS             complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNX27_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7881535.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNX27_000192"
     CDS             complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNX27_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7881536.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19776..20993
                     /locus_tag="JNX27_000193"
     CDS             19776..20993
                     /locus_tag="JNX27_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7881537.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21077..21676
                     /gene="yfbR"
                     /locus_tag="JNX27_000194"
     CDS             21077..21676
                     /gene="yfbR"
                     /locus_tag="JNX27_000194"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7881538.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21735..23567)
                     /locus_tag="JNX27_000195"
     CDS             complement(21735..23567)
                     /locus_tag="JNX27_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7881539.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="JNX27_000196"
     CDS             complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="JNX27_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7881540.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24315..24809)
                     /locus_tag="JNX27_000197"
     CDS             complement(24315..24809)
                     /locus_tag="JNX27_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7881541.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24892..25347)
                     /locus_tag="JNX27_000198"
     CDS             complement(24892..25347)
                     /locus_tag="JNX27_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7881542.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25685..26887
                     /gene="ackA"
                     /locus_tag="JNX27_000199"
     CDS             25685..26887
                     /gene="ackA"
                     /locus_tag="JNX27_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7881543.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26962..29106
                     /gene="pta"
                     /locus_tag="JNX27_000200"
     CDS             26962..29106
                     /gene="pta"
                     /locus_tag="JNX27_000200"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7881544.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29296..30816
                     /gene="yfcC"
                     /locus_tag="JNX27_000201"
     CDS             29296..30816
                     /gene="yfcC"
                     /locus_tag="JNX27_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7881545.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="JNX27_000202"
     CDS             complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="JNX27_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7881546.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="JNX27_000203"
     CDS             complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="JNX27_000203"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY7881547.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="JNX27_000204"
     CDS             complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="JNX27_000204"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7881548.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32836..33483
                     /gene="yfcG"
                     /locus_tag="JNX27_000205"
     CDS             32836..33483
                     /gene="yfcG"
                     /locus_tag="JNX27_000205"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7881549.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33540..33902
                     /gene="folX"
                     /locus_tag="JNX27_000206"
     CDS             33540..33902
                     /gene="folX"
                     /locus_tag="JNX27_000206"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7881550.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIVREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33923..34816
                     /locus_tag="JNX27_000207"
     CDS             33923..34816
                     /locus_tag="JNX27_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7881551.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34864..35754)
                     /locus_tag="JNX27_000208"
     CDS             complement(34864..35754)
                     /locus_tag="JNX27_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7881552.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="JNX27_000209"
     CDS             complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="JNX27_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7881553.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36732..37448)
                     /locus_tag="JNX27_000210"
     CDS             complement(36732..37448)
                     /locus_tag="JNX27_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7881554.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37445..38131)
                     /locus_tag="JNX27_000211"
     CDS             complement(37445..38131)
                     /locus_tag="JNX27_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7881555.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="JNX27_000212"
     CDS             complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="JNX27_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7881556.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39224..40006)
                     /gene="argT"
                     /locus_tag="JNX27_000213"
     CDS             complement(39224..40006)
                     /gene="argT"
                     /locus_tag="JNX27_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY7881557.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40272..40841)
                     /locus_tag="JNX27_000214"
     CDS             complement(40272..40841)
                     /locus_tag="JNX27_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7881558.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40936..42453)
                     /gene="purF"
                     /locus_tag="JNX27_000215"
     CDS             complement(40936..42453)
                     /gene="purF"
                     /locus_tag="JNX27_000215"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7881559.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEEENLEMHNEG"
     gene            complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="JNX27_000216"
     CDS             complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="JNX27_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7881560.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="JNX27_000217"
     CDS             complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="JNX27_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7881561.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43889..45157)
                     /gene="folC"
                     /locus_tag="JNX27_000218"
     CDS             complement(43889..45157)
                     /gene="folC"
                     /locus_tag="JNX27_000218"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7881562.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45227..46141)
                     /gene="accD"
                     /locus_tag="JNX27_000219"
     CDS             complement(45227..46141)
                     /gene="accD"
                     /locus_tag="JNX27_000219"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7881563.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46297..46956)
                     /locus_tag="JNX27_000220"
     CDS             complement(46297..46956)
                     /locus_tag="JNX27_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7881564.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47039..47851)
                     /gene="truA"
                     /locus_tag="JNX27_000221"
     CDS             complement(47039..47851)
                     /gene="truA"
                     /locus_tag="JNX27_000221"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7881565.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47851..48864)
                     /locus_tag="JNX27_000222"
     CDS             complement(47851..48864)
                     /locus_tag="JNX27_000222"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7881566.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="JNX27_000223"
     CDS             complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="JNX27_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7881567.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50165..51160
                     /locus_tag="JNX27_000224"
     CDS             50165..51160
                     /locus_tag="JNX27_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7881568.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMPTRPALWILLVAIILMLVWLVR"
     gene            complement(51157..52335)
                     /locus_tag="JNX27_000225"
     CDS             complement(51157..52335)
                     /locus_tag="JNX27_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HAY7881569.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="JNX27_000226"
     CDS             complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="JNX27_000226"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7881570.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53989..55995
                     /gene="mnmC"
                     /locus_tag="JNX27_000227"
     CDS             53989..55995
                     /gene="mnmC"
                     /locus_tag="JNX27_000227"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7881571.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56116..56394)
                     /locus_tag="JNX27_000228"
     CDS             complement(56116..56394)
                     /locus_tag="JNX27_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7881572.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56428..56976)
                     /locus_tag="JNX27_000229"
     CDS             complement(56428..56976)
                     /locus_tag="JNX27_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7881573.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56976..57785)
                     /locus_tag="JNX27_000230"
     CDS             complement(56976..57785)
                     /locus_tag="JNX27_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7881574.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="JNX27_000231"
     CDS             complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="JNX27_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7881575.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSPDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="JNX27_000232"
     CDS             complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="JNX27_000232"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7881576.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="JNX27_000233"
     CDS             complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="JNX27_000233"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7881577.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60831..61382
                     /gene="smrB"
                     /locus_tag="JNX27_000234"
     CDS             60831..61382
                     /gene="smrB"
                     /locus_tag="JNX27_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7881578.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61486..62376)
                     /locus_tag="JNX27_000235"
     CDS             complement(61486..62376)
                     /locus_tag="JNX27_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7881579.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62363..62887)
                     /locus_tag="JNX27_000236"
     CDS             complement(62363..62887)
                     /locus_tag="JNX27_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7881580.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62884..63354)
                     /locus_tag="JNX27_000237"
     CDS             complement(62884..63354)
                     /locus_tag="JNX27_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7881581.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63351..63899)
                     /locus_tag="JNX27_000238"
     CDS             complement(63351..63899)
                     /locus_tag="JNX27_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7881582.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63904..64626)
                     /locus_tag="JNX27_000239"
                     /pseudo
     CDS             complement(63904..64626)
                     /locus_tag="JNX27_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64646..67287)
                     /gene="yfcU"
                     /locus_tag="JNX27_000240"
                     /pseudo
     CDS             complement(64646..67287)
                     /gene="yfcU"
                     /locus_tag="JNX27_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67369..67932)
                     /locus_tag="JNX27_000241"
     CDS             complement(67369..67932)
                     /locus_tag="JNX27_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7881583.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="JNX27_000242"
     CDS             complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="JNX27_000242"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7881584.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="JNX27_000243"
     CDS             complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="JNX27_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7881585.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="JNX27_000244"
     CDS             complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="JNX27_000244"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7881586.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72938..73222)
                     /locus_tag="JNX27_000245"
     CDS             complement(72938..73222)
                     /locus_tag="JNX27_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7881587.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73594..74934
                     /gene="fadL"
                     /locus_tag="JNX27_000246"
     CDS             73594..74934
                     /gene="fadL"
                     /locus_tag="JNX27_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7881588.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75300..76351
                     /locus_tag="JNX27_000247"
                     /pseudo
     CDS             75300..76351
                     /locus_tag="JNX27_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        19317 a        20452 c        18740 g        17903 t
ORIGIN      
        1 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
       61 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
      121 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggag
      181 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
      241 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
      301 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
      361 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
      421 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
      481 atacacaatt agaaataaaa cactcacacc ccaaaaatag agcatccggc aaaagagtga
      541 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
      601 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
      661 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
      721 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
      781 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
      841 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
      901 aataatgatg aaataacgcc ttttattgat aatttacata tgaaaaaatt tttccttttg
      961 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
     1021 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
     1081 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
     1141 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
     1201 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
     1261 atcccgatcg gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
     1321 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
     1381 cagggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
     1441 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
     1501 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
     1561 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
     1621 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
     1681 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
     1741 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
     1801 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
     1861 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
     1921 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
     1981 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     2041 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
     2101 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
     2161 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
     2221 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
     2281 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
     2341 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
     2401 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
     2461 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
     2521 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
     2581 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
     2641 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
     2701 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
     2761 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
     2821 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
     2881 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
     2941 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
     3001 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
     3061 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
     3121 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
     3181 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
     3241 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
     3301 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
     3361 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
     3421 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
     3481 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
     3541 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
     3601 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
     3661 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
     3721 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
     3781 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
     3841 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
     3901 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
     3961 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
     4021 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
     4081 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
     4141 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
     4201 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
     4261 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
     4321 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
     4381 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
     4441 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
     4501 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
     4561 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
     4621 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
     4681 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
     4741 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
     4801 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
     4861 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
     4921 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
     4981 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
     5041 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
     5101 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
     5161 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
     5221 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
     5281 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
     5341 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
     5401 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
     5461 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
     5521 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
     5581 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
     5641 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
     5701 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
     5761 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
     5821 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
     5881 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
     5941 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
     6001 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
     6061 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
     6121 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
     6181 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
     6241 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
     6301 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
     6361 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
     6421 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
     6481 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
     6541 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
     6601 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
     6661 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
     6721 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
     6781 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
     6841 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
     6901 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
     6961 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
     7021 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
     7081 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
     7141 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
     7201 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
     7261 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
     7321 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
     7381 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
     7441 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
     7501 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
     7561 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
     7621 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
     7681 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
     7741 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
     7801 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
     7861 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
     7921 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
     7981 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
     8041 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
     8101 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
     8161 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
     8221 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
     8281 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
     8341 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
     8401 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
     8461 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
     8521 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
     8581 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
     8641 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
     8701 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
     8761 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
     8821 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
     8881 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
     8941 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
     9001 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
     9061 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
     9121 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
     9181 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
     9241 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
     9301 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
     9361 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
     9421 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
     9481 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
     9541 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
     9601 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
     9661 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
     9721 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc
     9781 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
     9841 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
     9901 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
     9961 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    10021 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    10081 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    10141 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    10201 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    10261 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    10321 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    10381 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    10441 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    10501 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    10561 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    10621 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    10681 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    10741 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    10801 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    10861 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    10921 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    10981 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    11041 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    11101 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    11161 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    11221 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    11281 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    11341 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    11401 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    11461 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    11521 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    11581 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    11641 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    11701 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    11761 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    11821 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    11881 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    11941 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    12001 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    12061 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    12121 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    12181 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    12241 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    12301 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    12361 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    12421 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    12481 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    12541 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    12601 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    12661 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    12721 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    12781 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    12841 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    12901 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    12961 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    13021 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    13081 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    13141 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    13201 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    13261 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    13321 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    13381 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    13441 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    13501 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    13561 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    13621 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    13681 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    13741 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    13801 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    13861 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    13921 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    13981 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    14041 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    14101 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    14161 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    14221 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    14281 aacaaaatcg atactgccca gataaacaat caggtcagac accaagctgc cgcggatcgc
    14341 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    14401 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    14461 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    14521 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    14581 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    14641 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    14701 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    14761 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    14821 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    14881 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    14941 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    15001 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    15061 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    15121 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    15181 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    15241 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    15301 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    15361 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    15421 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    15481 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    15541 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    15601 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    15661 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    15721 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    15781 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    15841 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    15901 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    15961 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    16021 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    16081 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    16141 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    16201 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    16261 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    16321 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    16381 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    16441 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    16501 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctggcgcg gcgaagcacg
    16561 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    16621 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    16681 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    16741 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    16801 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    16861 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    16921 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    16981 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    17041 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    17101 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    17161 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    17221 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    17281 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    17341 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    17401 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    17461 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    17521 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    17581 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    17641 gatctgtgac acgcttttgt tattccatag caaaaacgca gcaacaaatt tacgtatttt
    17701 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    17761 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatccgag gaaggatttt
    17821 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    17881 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    17941 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    18001 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    18061 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    18121 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    18181 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    18241 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    18301 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    18361 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    18421 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    18481 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    18541 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    18601 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    18661 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    18721 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    18781 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    18841 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    18901 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    18961 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    19021 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    19081 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    19141 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    19201 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
    19261 gcgaattatc tgatgcaaag ttatgttatg ttatgttatg ttaggcaaag taatcttcta
    19321 ttattaataa gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc
    19381 tcattcatta attccgctta acaatagttt cacaccttac ccccattgcg acacctcgga
    19441 aatcatcact ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg
    19501 aattcttaaa tgaggcattt tcacacaatt atcttacaga taaaaaaacc atacttgcaa
    19561 ttaagaatta gaacaattca ctatataaca ttacatgtaa agcatataca cctaattatt
    19621 ttgtcattat taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc
    19681 tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca
    19741 gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca
    19801 aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg
    19861 aagaagaagg taacaaggta ctgaaactga acatcggcaa cccagcccca ttcggttttg
    19921 acgcgccaga tgaaatcctc gttgacgtga tacgcaacct gcctaccgct caagggtatt
    19981 gcgattccaa agggctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg
    20041 gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg
    20101 ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcaccagatt
    20161 acccactctg gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg
    20221 atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta
    20281 cgcgtgggat tgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc
    20341 ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa
    20401 tttatgacaa aattctctac gacgatgctg agcatcactc aattgcgccg ctggcacctg
    20461 acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc
    20521 aggggtggat ggtgttgaac gggccgaaaa aacacgccaa aggctacatc gaaggtctgg
    20581 aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg
    20641 cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc
    20701 agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc gtgaaacctc
    20761 gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt catgacgatc
    20821 agaaaatggt gctggatttc ctgttgcagg aaaaagttct gttggtgcaa gggacggcat
    20881 tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg
    20941 agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat
    21001 ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat
    21061 catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact
    21121 cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt
    21181 gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcggaaat ttggcggtaa
    21241 tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcctcag aagtgctcac
    21301 cggcgatctc cctactccgg tgaaatactt caatgcgcaa atcgctcagg agtacaaggc
    21361 tattgaaaaa atcgctcagc agaaactggt cgatatggtt ccggaagaac tgcgggatat
    21421 ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca
    21481 ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga
    21541 attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat
    21601 ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca
    21661 ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc gccggatgcg
    21721 acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc
    21781 acgataatgg tgaacggtac ccccaacttc acaaagtcac tgaagctgta atttcccgga
    21841 cctaaaacca gggtgttaac aggtgaagac accggtgtca taaaggcggc ggatgctgcc
    21901 atcgccacga ccatcgcaaa tggataaggc gacactccca tcgttttggc agcagccagc
    21961 gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaacagccc aataaccgcc
    22021 gacaagacaa acaaacaccc cagcatcata tgtggcccat aaccgccgcc aatatccatc
    22081 agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc
    22141 atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct
    22201 atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt
    22261 tcatttgtca gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg
    22321 ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg
    22381 ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc
    22441 agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg cttgagcccc
    22501 accacattca gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt
    22561 tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa
    22621 tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg
    22681 acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca
    22741 ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagcg
    22801 ccataacgct cacgtaattt gagatcatcc agccgttgac ctatcattgg cgatccgggg
    22861 cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag
    22921 gttcgacgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc
    22981 atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta
    23041 aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac caggttcggc
    23101 ggcgtcgcca ccagcgtcat catgccgcta atcagcccgg caaaacttaa cggcatcatc
    23161 agacgtgacg gcgaagtttg catacgcatg gcaacgctta acaccacggg aataaagata
    23221 gcgacaacgc tggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa
    23281 accaacattt tgatttcact gttgcccgcc actttgacca gccatgttcc cattacggtg
    23341 gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg
    23401 ttaggatcag aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac
    23461 gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg
    23521 atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt
    23581 taattgaggg ggatgacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc
    23641 atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt
    23701 gctccagact gtggaggacc aaatcgacct cattcaggcg cggggtatct gccggagcgt
    23761 taaccgcaat gacatggcaa cccgccgcta ggccagaaag cacgccagcg ggagcatctt
    23821 ccaccaccac acactcctgc ggcgcaagcc ccagcagctg cgcacctaac agatacgcat
    23881 caggttctgg ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa
    23941 gccccgctat tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc
    24001 acggaatacc tgctttattc aaatgattga gtaaggcgat tgccccagga agcgcggtaa
    24061 taccttcggt ttccgtggcc tcgatttgct ccaaacgcgt aaactcggcg gcaatatcag
    24121 cctcggattt gcccgccata aaatggcgca aagaggtgat cgcctgttta ccgtgaatga
    24181 aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt
    24241 ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc
    24301 gcacgcgggc ctcctcaggc gttgataatt tgattaattt cgttggcgct caaatggtac
    24361 tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc
    24421 tgggcgttaa aaccatgagt cccagcgtca aaatgggtat agcgcccttc cacattaacc
    24481 ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg
    24541 acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca
    24601 tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac
    24661 tcgcgatcca attcacgcat ctgtaatccg taaccacgct caataattgt ttgcaggcga
    24721 cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa
    24781 ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga
    24841 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
    24901 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
    24961 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
    25021 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag
    25081 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc
    25141 aagcagaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
    25201 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
    25261 tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt
    25321 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
    25381 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
    25441 actatcaggt atcctttagc agcctgaagg cctaaatagt acatattcat tgagtcgtca
    25501 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
    25561 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
    25621 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
    25681 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
    25741 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
    25801 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
    25861 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
    25921 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
    25981 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
    26041 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
    26101 acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
    26161 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
    26221 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
    26281 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
    26341 tatccgcaac ggtaaatgcg ttgacacctc tatgggtctg accccgctgg aaggtctggt
    26401 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
    26461 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
    26521 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
    26581 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
    26641 gctgatggat ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc
    26701 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
    26761 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
    26821 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
    26881 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
    26941 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
    27001 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
    27061 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact
    27121 acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc
    27181 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
    27241 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
    27301 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
    27361 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa
    27421 ctgacccgca acagcttcgg tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
    27481 aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc
    27541 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
    27601 ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
    27661 gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc
    27721 gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc
    27781 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca
    27841 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
    27901 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
    27961 accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac
    28021 gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
    28081 atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
    28141 tatcagctga ctgaactggc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
    28201 gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
    28261 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt
    28321 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
    28381 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac
    28441 gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
    28501 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
    28561 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
    28621 gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc
    28681 gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
    28741 ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
    28801 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
    28861 gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgttcatc
    28921 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg
    28981 atctccatcg ggccgatgct gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc
    29041 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
    29101 cagtaatctc gtcatcatcc acagctttgc gctgcggata tctgaaccgg aaataatcac
    29161 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
    29221 gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg ttttgtgcaa
    29281 gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg
    29341 cccgatacgt tggtgattat cttttttgtt gctattttaa ccagtcttgc cacctgggta
    29401 gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
    29461 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
    29521 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc
    29581 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    29641 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    29701 atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt
    29761 attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc
    29821 attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    29881 acttatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    29941 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    30001 tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    30061 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    30121 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgacagcc
    30181 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    30241 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    30301 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    30361 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggttggtaa tggtgaagca
    30421 ggtaatgcca gcgtgttaaa tactatcctc aacagcattg ccaatgccat tagcggtctg
    30481 gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg
    30541 acgtccggtt ctggtcaggc ggcattaact atgccgttac tggcaccgct tggcgatctg
    30601 gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg cttcagccac
    30661 atcatttacc caacctcagc ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc
    30721 cgtaactggc tgaaggtggg cgcgaccctg cttggactgc tgtttattat gtccagcgtc
    30781 gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    30841 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    30901 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    30961 tccggcgtca gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga
    31021 gagacgcaac tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    31081 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    31141 gattccagca gttgctcatc ggcctgaact acaccgcctg cggtcgcatc taacatgccg
    31201 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    31261 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    31321 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    31381 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    31441 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    31501 acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata
    31561 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    31621 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa
    31681 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    31741 gttatcggga aatgcagcat catttgatcc acttcgctgt cgcagttgcc acgcacagcg
    31801 ataaccttat gtgccacttc attaagccgt tcagcaactt tggctggcgc gtaaccctcc
    31861 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    31921 ccgctttgga caaacaactc cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    31981 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    32041 attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga
    32101 cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg
    32161 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    32221 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    32281 ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg
    32341 acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg
    32401 cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc
    32461 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    32521 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    32581 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    32641 taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat
    32701 tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    32761 cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa
    32821 acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt
    32881 acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa
    32941 ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt
    33001 gttgatcatt ctcctgccga tggcggcgaa ccgttaagcc tctttgagtc tggtgccatt
    33061 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    33121 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    33181 catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt
    33241 gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga
    33301 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    33361 ctgcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    33421 cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt
    33481 taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa
    33541 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttgcgtacg tttatcggaa
    33601 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc
    33661 ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa
    33721 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    33781 aggatgtgct cgatatcgta cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    33841 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    33901 aatcgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg
    33961 tcgccatttg attccgcgtt tgctggagct gggccatcaa attacggtag tgacgcgtaa
    34021 cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag ggcttgccga
    34081 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    34141 taaacgctgg actcacaagc aaaaagagcg tctctgccaa agccactgga atatcacgca
    34201 aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc
    34261 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    34321 taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag
    34381 tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat
    34441 tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    34501 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    34561 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    34621 tgcccatgcg ctcggtcatg cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc
    34681 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    34741 taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc
    34801 ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc ctgtgttaca
    34861 ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    34921 ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt
    34981 gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc
    35041 ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt
    35101 ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag
    35161 cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat
    35221 taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca ccactgtaat
    35281 atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc
    35341 atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg
    35401 gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga
    35461 ataacgcatc atgcgaaaag ccattaattc ttccggctta ctttggtgct ctatcactac
    35521 ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg
    35581 ccgcaggtct tcatcaataa aactgtttgg ttctagttta agcgtcgtta aatcacacag
    35641 tttgcgcagc ggcgcgggaa gatgaatatt aataaaattc cgcgcggtgt ctggatggcg
    35701 taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt
    35761 ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc caatgcagca
    35821 ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg caacttttac
    35881 atttcagcgg aagctgactc gcaaattccc cttgaaaata ctccggcagc gttagccacc
    35941 ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg ctttgcgggt
    36001 tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgg aggaaaatga
    36061 cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc atggttttcc
    36121 cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca ggatcgagcg
    36181 ccgaggtagg ttcatcaaac agtaaaactt ctggttccat cgccagcgcc cgcgcgatag
    36241 aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc gcacgttcgt
    36301 ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc
    36361 ccaacacctg aatcggcgct tccatgacgt tttccaacac cgtcatatgg ctccagagat
    36421 tgaagtgctg gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg
    36481 cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca ttgaccacga
    36541 tcgatccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg
    36601 atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt gagacccctt
    36661 tcagcacttc gtgttcgcca tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg
    36721 acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttccgct
    36781 ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc ggcaatgccg
    36841 aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt cagcaaatcc
    36901 ggcacagtgg ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac ttcgttgctg
    36961 tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata cattttaaag
    37021 gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga acggattgcc
    37081 ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag
    37141 ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat ctcaagcgtg
    37201 tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag
    37261 gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc aatcgccaga
    37321 aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag agtgatcgcc
    37381 acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc atgtaagatt
    37441 tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca
    37501 caccattgga aacggtggtg aaaaccaggt aaatcacgcc acagacgatg gcgaaataga
    37561 acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct tccaggccga
    37621 gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca
    37681 gggcgtaacg catcatcgcc ggaaacatga tcctccgaaa cacttgcccg cgagtaaaac
    37741 cgaacgccgt cgccgcctct atatgccctt tcggcactgc cataaacgcg ccacggaacg
    37801 tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg accatcggat
    37861 cgatatcaat ctgcccgacg cccatcgcct ccgtcaccat gtttagcgca atctgcaaac
    37921 catagaaaat cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc
    37981 cttcgaaaat cagccccaaa agccgatttt gcgagagctt accgccagcg ccaattaaac
    38041 cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg agcgcaccct
    38101 gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt
    38161 accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga
    38221 ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc
    38281 agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg
    38341 caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg gaccaccgaa
    38401 tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggcagcga cctcatcctg
    38461 gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata
    38521 cgagacgatt tcaatgcctt ttggtgccca gtgttcatta ccgaacgtct cctgggtggt
    38581 gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct gaatgtcaga
    38641 atttttcgcc accaccagac gagaatcggc agcgtacagt ttgtcggtga aggcaatttc
    38701 ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt
    38761 taaggacggg attaacgcat ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg
    38821 cttgcacaac tccttcgcca gctcgatatc gaaaccaacc agttcgcctt gtgaattttt
    38881 tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg gaattgcagc
    38941 aagtgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt
    39001 cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg ttacacttat
    39061 cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgttagacgt attatttttc
    39121 attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc
    39181 accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca ccgtagacat
    39241 taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa
    39301 gcgccttatt gaaggcagcc gtcagttcag catcatcttt acgtagccct acaccggtgc
    39361 catcaccgaa gtattttttg tcttttactg atgagccagc aaaggcgaaa tctttaccag
    39421 caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca gcatccagac
    39481 gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc acatccacgc
    39541 ctttactacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa
    39601 catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcagcaatca
    39661 aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg
    39721 taatggaaag cgacgaaata atagcgtcga tttttttcgc tttcagtgag gggagcagcg
    39781 cgtcaaagtc actggcaacc caggtacatt tcacctgcat ccgtttgcac atctcgttac
    39841 cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag aacggcgcat
    39901 aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg
    39961 cggagagacc gactaataaa gacagagcga gaatcgactt cttcatacat aaccctcaag
    40021 tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg
    40081 tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa taagacgttg
    40141 cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg
    40201 aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg
    40261 caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttattgca
    40321 aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga ttgcgggcga
    40381 tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt
    40441 aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc
    40501 tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg
    40561 ccggacaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg
    40621 gaagagatgc tggcggaaat atcgcgcgca tcgagcgtga catcagctaa tgcctgcact
    40681 tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcatcacc
    40741 agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac gccataaatc
    40801 gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga
    40861 cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc cagaccggca
    40921 ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttcctctt cgttctgacg
    40981 ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt agccctgatc
    41041 aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg
    41101 attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca acccgtcagc
    41161 accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct
    41221 cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta
    41281 cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg
    41341 gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt
    41401 cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa aggtgcggcc
    41461 aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac gagcaatttc
    41521 cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat ccagatcttc
    41581 ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac
    41641 ggaaattttg tcgataaacg agtccgggcg ggcaaagtat acatactcaa acaggcacgg
    41701 attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt cagtgatgta
    41761 aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac
    41821 gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt ttcccagtac
    41881 cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga taatcatcgc
    41941 cacacaggca tacgcgccgc ggattaagcg gtttgtggca gcaatggcag cgaaaatatt
    42001 gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga tattaagcag
    42061 aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg
    42121 caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggggagtt
    42181 aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg
    42241 accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac
    42301 cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga tgatgccggc
    42361 ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg
    42421 cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc
    42481 tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat caaaaaacca
    42541 tctgatgata aaactgaatt gcgggatcag ctgtgatttg ctccagtctt cacttttcga
    42601 caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca caccgcgcaa
    42661 cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt tctccaccaa
    42721 ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc
    42781 gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta
    42841 agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc
    42901 acgaacaaag ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc
    42961 gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg
    43021 cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa
    43081 tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca tcgtgcaatg
    43141 cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg cccaagcctg
    43201 ataagacgcg ccagcgtcgc atcaggcaag actgtgttaa ttcggcgtat agcccattac
    43261 cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca gcttatcttt
    43321 cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga
    43381 cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc
    43441 atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg ctttttcttc
    43501 cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg gtttaggcgg
    43561 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    43621 attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    43681 ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc
    43741 aggctcatca cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    43801 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    43861 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    43921 cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg tcttccgctt
    43981 tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac
    44041 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    44101 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    44161 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    44221 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    44281 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    44341 tgacttccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    44401 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    44461 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg
    44521 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    44581 ggaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggacccagc cagtcggtat
    44641 gatccagcgc aatactggtt actaccgcgg catcggcatc gacaatattg gttgcgtcca
    44701 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc
    44761 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    44821 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcccggccc tgcacacgta
    44881 cgcgctcggt ataacgcacc agatgaggcg aactgtagac gcccactttg taccctgccg
    44941 ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg
    45001 taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    45061 caaggccgag ctcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    45121 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    45181 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    45241 cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    45301 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    45361 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat ctaggcggca
    45421 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttag
    45481 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg
    45541 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag
    45601 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    45661 cggagaagca gatcagcggg cagttatctt cctgcgcctg ctcaacggca cgcacgaaac
    45721 gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga
    45781 caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    45841 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagctcat
    45901 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    45961 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    46021 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    46081 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    46141 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    46201 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    46261 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    46321 gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa taatcccgac
    46381 gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat agcctgcgta
    46441 ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcagcga aatgacggta
    46501 cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg
    46561 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt tgtcgagata
    46621 actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga acaaccgccc
    46681 gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca ctaccatcat
    46741 atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa acagcaatga
    46801 atcacccggt aaaaacggca ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    46861 aatggcataa acccagacgc cgtactccgc gactagctct gccagatgca cgtcaatgtg
    46921 caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc
    46981 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt
    47041 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    47101 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    47161 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    47221 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatcaacca
    47281 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    47341 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    47401 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    47461 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    47521 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    47581 aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat
    47641 gcaccacttg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga
    47701 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    47761 cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt
    47821 tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    47881 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagag
    47941 ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt
    48001 acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    48061 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    48121 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    48181 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    48241 accttcgcta tccggcaaca acggcagcat attgaacgcc agttgacgac cgaagaaatc
    48301 ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta acgcatcgac
    48361 cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac gcgacaaacc
    48421 gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtctggtac
    48481 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac
    48541 cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca ggcaacctga
    48601 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    48661 ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgttt taccaccaaa
    48721 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    48781 ctgacgttca gccagcgttt caagcagggc ttcgcccata gcgccagttg cgcccaggac
    48841 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    48901 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    48961 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    49021 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    49081 actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    49141 agcgtcggtt gatcgagcgg accatgcagc gtaatgcggc caaactcagg cgcgggcagc
    49201 aacgtatcca gcgcaacgtg ttgttcatgc ccaataaact tgctataggc ttcaaacact
    49261 tgcgtcgtgc cacgcgcttt accctccagg gtatagcctg cgatatgcgg cgtgccgata
    49321 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    49381 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    49441 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    49501 gccagatgta gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    49561 tccgcgcgct ggactaactc atccagcgag cggaaatcac cctcatcccc acggtcggcg
    49621 cgaggcggat cgcaaagtaa ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga
    49681 cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg
    49741 cgttcggcaa gcatcagcag ggaggagaaa acatattcca ccaccgcaat cgcattacag
    49801 ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc gacatggtcg
    49861 gtccccgcag tggcagttcc aacaaattta atgggttttc ctgccagcaa agattcattc
    49921 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc gacggggatt
    49981 ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    50041 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    50101 gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    50161 cgtaatgata caacctattt ccggccctcc tcctgggcag ccaccaggtc agggagataa
    50221 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    50281 cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt gggcgggtat
    50341 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    50401 tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    50461 tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta
    50521 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    50581 cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc agcgcgaggc
    50641 gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg tgaccaaact
    50701 tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    50761 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctaca
    50821 ggcgcgtcag acgctaagcc agcaaaatac gccgacgctg gaatcactac agatggcgct
    50881 aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    50941 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    51001 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt
    51061 tccaccgatg atggacacgt tgcaaaatat gccaacgcgt cccgcgctgt ggatactgtt
    51121 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa atgacagtat
    51181 cgtgacaata atacccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    51241 aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga tatcctgaaa
    51301 cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt taaccacctc
    51361 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    51421 tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    51481 agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    51541 catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg cccatccttt
    51601 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    51661 cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttctcc
    51721 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatac
    51781 cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg
    51841 cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca ctttgccaga
    51901 gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta actggctttc
    51961 accaaaccca agaatcaaac gcccgatgac caacagggca aatttgaacg gtgcggagac
    52021 aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta acatcccctg
    52081 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    52141 caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc catagcccag
    52201 atcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    52261 aaaaaccgca aaagcgatgc ggaagagcga aaaactggca gaagatcgtg tttcggtttg
    52321 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    52381 atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga
    52441 aggaaatcga caaagcggga agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa
    52501 aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc
    52561 ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat taatctttca
    52621 gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacag
    52681 tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc tgctcgtcca
    52741 gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga
    52801 tagcttcctg cacacccgca gcgcccagtg agtgaccggt catggctttg gttgcagaaa
    52861 tcgccgggct cttatcgccg aacacttcac ggatagctgc cagctctttc acgtcgccaa
    52921 ccggagtcga agtaccgtga gagttcaggt aatcgattgg ggtatcaacg ccatgcatcg
    52981 ccatctgcat acaacgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag
    53041 aggttgcgcc gtagccaacg atttcagcat agatgtaagc accacgcgcc agcgcgtgtt
    53101 ccagctcttc aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag
    53161 cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca
    53221 tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga
    53281 tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg
    53341 cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgagacagg
    53401 cagaaacgcc ggatgccatc gctttagtga ccacatacgg gccaaccgct ttcaggccgc
    53461 gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg cggggaaccg ccgccggaac
    53521 ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagagagg cccgcatcag
    53581 cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa
    53641 ctttgcggtc aatgaggcca gtgatatcca gttttatgtt gccccagacg tggctacgca
    53701 tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg
    53761 ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg
    53821 cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc
    53881 gtacacttgt acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt
    53941 acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac
    54001 tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt
    54061 gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga
    54121 ggtaaccaat tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag
    54181 agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc
    54241 gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc
    54301 ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca
    54361 gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat
    54421 gaaggccgcg tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaactg
    54481 gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagctaaa
    54541 aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc gcgtccaggc
    54601 ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggaagccgga
    54661 ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg tggggtgatg
    54721 gaacagacat taccactccc ctgctccacg ccgtggttta atcgtacggg cagcagcaaa
    54781 cgggaagtag cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgttatta
    54841 cggcgcggct ggcaggtagc gctttattgc gcggatgaag cccccgcgct gggtgcttcc
    54901 ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc
    54961 tttttctcta atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt
    55021 gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc
    55081 gcacagatgt tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg
    55141 gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt
    55201 tggctgtgcc cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg
    55261 cagatttatt atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg
    55321 aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa
    55381 atcagccgtt tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc
    55441 catattccga caacgccgga actggcagag ctgaagcagg tgctgtgcta tgacggttat
    55501 ctcacgccac aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc
    55561 agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat
    55621 tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc
    55681 ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag
    55741 gcaacactcg tggaatatgc gtcgttggcg gagcaaaaag atgaggcggt aagcgcgccg
    55801 gtttttgacg atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg
    55861 ctgtgtgccg agattctggc ggcacagatg agcgacgaac cgattccgat ggatgccagt
    55921 acgctggcgg cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg
    55981 gttaaggcgc ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc
    56041 aacctacgtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc
    56101 tccagacgcc gcaacttaca gctgtgacgc ctgctgaaac aggttttccc acatatcggt
    56161 caccagcgcc tggtcacgcg gcgacaattc accggcacca atggcttttt ccagactctg
    56221 gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca gttctgcgat
    56281 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    56341 gtcaaccatg ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    56401 tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc
    56461 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccgggcg
    56521 ttccggaatg ccatttgtca gataatccat cacctgcgca tgtacccgcc gttgaaacac
    56581 cacacgatcc ggttcgaaat ccccttccag attgtcgcaa ctgacgttaa acggatatcc
    56641 agccgccacg cagaataacc agtcgaacgc ctgcggcttc acttcaacat cttcaaactg
    56701 gctttgcgtc tgggcatcgc gtccgtccgg gcaataccag tagccgaaat caaccagctc
    56761 acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa
    56821 gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg
    56881 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    56941 ttcaatcaac tgctcgtagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    57001 atctcctgtc cgtggctatc gtaaagtaat ttggcactca tcaccgccga gacgataaca
    57061 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca accgtgaacc catgcgcgcg
    57121 ccgaggaact gccccaccag catgacaaag cccgtcgccc aaatcacttt gccgccgaga
    57181 ataaacagca gcagaccgcc aatgtttgac gttgcgttga gtaatttggc gtgagccgtg
    57241 gctttggcga ggttgaatcc gcacagcata acgaaggcca gcgcgtaaaa cgatccggca
    57301 gccgggccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa cgcgaagggt
    57361 aaaccgtaca tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag
    57421 aggccgatac aaatcaccag aatgggcaaa atctgccgta agacatcagc ctgaacgtac
    57481 tgtaccaaca gtgcgccgct cattgagccg acaaaggtca tggcaatatt gagtttctga
    57541 tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg
    57601 caggcttgca gtttattggt tgccagcgcg ttagcgggag acattcccgc agccatcaat
    57661 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg
    57721 gcgacaaaaa agagaactcc cagcaacagc ggagaaacca taaacaggct attaaacgtt
    57781 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    57841 tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt tgcagttctg
    57901 ccccgcaacc atcgcctgat ggcggtaaag gttgatcttc acactccaga ctatcggcag
    57961 ggcaacgtaa tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc actttgcgca
    58021 accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa
    58081 aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat caagctgaaa atttctggct
    58141 tccacagcgt ggagacaacg tgtttaccgt cgggggaaac taagtccagt gcttgcgggc
    58201 gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata tcgacatcca
    58261 gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcgggc atccccatat
    58321 cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga cgctggataa
    58381 acatcaccag atccgggtga ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    58441 gttcggactg tatcggcaac gtgtcagcgc cgacaataca gccattagaa aaactgccta
    58501 tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    58561 tagcgctact ggcaagcaga gccagcagcg caatcgcggt tttgttcatt ttttaccagc
    58621 gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg tgatccatta
    58681 aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg gatcccgaca cagggatcgt
    58741 gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg cgccccggca
    58801 cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat gatttgctgc ccgctgctga
    58861 taccgccgaa aatgccgccc gcatggttgc tctggaaacc ctctttggtg atttcgtcgc
    58921 ggttctggct gccgcgcagc gccactacgt caaaaccgtc gccaatttcc acgcctttca
    58981 ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca aagaccggct
    59041 cgccaagtcc ggcggggaca ccactggcaa caacggtgac tttcgcgccg atggagtcgc
    59101 cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcg gggtccgggc
    59161 agaagaacgg attttgttca accagcgacc agtctttgat ttccagcgga atgtcgccca
    59221 tctgggtcag gcagccgcgg atttcaatac caaatttctc ggcgagatat tttttggcaa
    59281 tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtcca ccgccgcgat
    59341 aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc atggcctgga cggaagacgt
    59401 ctttaattgc gctgtaatcc tgagaacgct ggtcggtatt ttcgatcaac aagccaatgc
    59461 tggtgccagt agtaacgcct tcaaaaacac cggagagtat tttgacctga tccggctcgc
    59521 ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg atcaaggtca tgttgcaggt
    59581 ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc
    59641 cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca
    59701 cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatgttct
    59761 cgtgcggcaa gaagctgctc tttagtgagc ataaacacac catcgccgcc gttatcaaac
    59821 tccagccagg tgaacggaac atcgggatat tgttccataa gatgtaccat gctgttgccc
    59881 acttcacaaa tcaacacgcc atcatcagca aggtaatctg ccgcgttacc gagaatgcga
    59941 cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca gttccggctc gtgacggtat
    60001 tcgtttggca ggtcggacat atcttccgcg tcgacatacg gcgggttagt gacaatcaga
    60061 tcgtattgca ctttcggcaa atcacggaac agatcggaac gaatcggaat gacgttgtgg
    60121 atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg agagatatct
    60181 accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat gcagccgcta
    60241 ccggtacaca tatctaaaat atgctgcggt tgcttgctga tgagtccggc aaatttattg
    60301 ttgatcagtt caccaatcgg cgagcgcggc accagcacgc gttcatcgac gtaaaattca
    60361 tgaccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg
    60421 cggatcacgc gttcaacaat acggtgtttt tcgctggagg tcagacgcgc ggtgcgcata
    60481 tcttccggga tatccagcgg caggtagagc gatggtaaca ccagctgtac ggcttcatcc
    60541 cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg gcttaccgac
    60601 cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta
    60661 tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat
    60721 gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt
    60781 gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagataa atgaaaaaga
    60841 aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg gggactcgca
    60901 agattaagca ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg
    60961 tgaaacgctt gatccaggag caggctgatg ccagccatta tttctccgat gagtttcagc
    61021 cgttattaaa taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc cattttgagg
    61081 cgaagaaact gcgccgcggc gattattcac cggaattgtt tttggattta cacggtctga
    61141 cgcagctgca ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg
    61201 tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa caaacaccgc
    61261 tgtggctggc acaacatccg catgtgatgg catttcacca ggcaccgaaa gagtacggcg
    61321 gtgatgcggc tttgttggtg ttgattgaag tggaagagtg gttgccgccg gagttgccct
    61381 gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta tccggcctac aaatatgtga
    61441 taattcaata aattgcaatt cacttcgtag gcctgataag acgcatcaga gcaatactac
    61501 gaacttcagt tcattcaata catactgaaa acgacacgtc gaatcaatcg gttgcccact
    61561 actggtattc acattcacca caatatcgtg tagtacttta tccgttaccg gatgacgagt
    61621 tggcgtaacc gttaccgttg atttatcaca accgtaagag gtaaaggtta acaattctga
    61681 taccgcgcgg cagtatgctc ctctgccatc attatcagaa aaattactgc catcacagag
    61741 tctgccgcct tccatcaggt aggtcgaaag cgtggtggtc actgttgacc ccgtggactg
    61801 aattcgtaac gccttattat ggctgacata attttcactc caggagatat cgtatgaagc
    61861 gaccggtatc gttgggcata ttgtgttatg cactgtaggg gccgtatagc ttccaaatcc
    61921 gggtaaatct gtataggaat aaaaggatgc gatccaaggg caaccataat catcagatgc
    61981 aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt ttcgtatcta tgccagggca
    62041 atttgagtta ttaattgtat caaagtcagt cagtaccaaa gttggagctg aacccgaccc
    62101 agaccaggaa ataacccgca acgtatcgcc tttatacgca aaagaatcat atttcccata
    62161 tgcaacctcc tttggcgtta tcaccgtaac attgaaaaca ctgctcgcta cagcaccggt
    62221 taccttgtcc atggcataat aaactctcac actgtcaaaa ctcgcactca taagtttatc
    62281 tccagcataa ctcttagatg cgccgacaac actcagaacg agcagcatta ggctcgcgaa
    62341 aattatggtc cattttttca tttcagaaat actcaaaggt tgccgtcact gtggcactaa
    62401 agtctccgga cgttacatct cgcccgctct ttgcctgtaa ccaggtatta aaatttaggc
    62461 tgttatttcc ctgattcagc gggagcgtga cgcctgttgt attgttgatt ggcaccgatg
    62521 tgcctgccgc agtttcaata ccaatcccag cgccggatgc tgatgacgag gagtcaaagg
    62581 ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa cgttactttg acattaaaca
    62641 atgtagaact atgacaatct ttcaattgaa acaccaatgg cactcgctct gactgcccaa
    62701 ggttcattaa atcgctggcg gcaatggcag gaaaatgtac ttctgccaac tggctaccat
    62761 taatcactgg cgtacacgat ttactgatta aattgccggt aaagtgcaaa ttatctcctg
    62821 ctattgtggt tccactgccc agcaacatca ggctactaat aaaaaacaac gtacgctttt
    62881 tcttcattgg taatctacct gtaacgtggc ccaggcctca aagtccgcct ctggcagaat
    62941 gacaccactc ttttttactg gcacagccgt taacaccggt aaatcggttt cattcaccac
    63001 aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg agctgaatgc ctaaaccagt
    63061 gatattgctg cttactgcag caatatcaaa atcgcttggt gtgccactcc agctcaaggt
    63121 catttccatt aatgggtcgc gaaccgccgt atcgcaggta atggaatacg gcacggtttg
    63181 ctcataattg ctgccattaa tgtcatcaat aagcacatcc tgaaaagtta tttcaatggt
    63241 ttgagcgttg ctaatcgtgc agttaggcgg ctgcactaaa taaccgtgaa aagagacgtt
    63301 tgagagtgcc atcgagcaaa agccccacag caaaataagc gagatccgtt tcattggtaa
    63361 tccaccacaa ttgtggcact ggcgttaaat tcagctgccg ctaacgttgt accactctgt
    63421 ttcaccggca cagcttccag aacgggaact cccgaggaaa aattaaatgg caaccatgag
    63481 ccagaggtta agtcgagtat tgtgtgatca ctacttttct gaacccgaat accaaaccca
    63541 ggaatacccg tgctgataac tgtttcaccg ttaatcacga ccgttgtcgc ctgcatctgt
    63601 agacgcaggt catccattgc cagattgtta caaacaagcg agtagtcaat gggacgttta
    63661 taatcaaccc tattgatctt attaatgaat acattaccaa actcaactgc cgcaccattt
    63721 accgtacaag gaggaggggc atcgacaagc acccgtaaat tcatacttac tgacttactc
    63781 gctgcaactg caaagccgct accaacacaa catatcagta acccagttac cgcccagaca
    63841 gaacaagatc ctttcatact ggctctcccg gcctcagcct ttgcggctct gctcttcatc
    63901 gacctagcaa ctgttaccgt tgcaagtgaa aaagagcggc atccgagcgc catagtcgtt
    63961 gacataggtc agtaccggca cgctacccat cttcacattc agcggtgcat aggttttcgg
    64021 cgacatcact attggcgaga aaccggctgc cggattgcca tttttctgtg tgctggcatt
    64081 actgaaaatg acgtaatacg acgtcgggtt attgacggta aattcctgac cgttgcgggt
    64141 taaagtaacc ttatattgct aggggttttt catgctgacg ttctccagcg ccttcggtcg
    64201 ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc gtgttggctt tgtttgattt
    64261 tggcgggatc tcacgcacgt tgaagtagaa caggctttca cgatcggcag gcaatttgtt
    64321 gatatccggc attccctgca cttttacctg cccgttcatc attgaatcga tgcgttgcac
    64381 cggtggcaga acagttaacg gtgaactgat cttgttaccc ttttcatctt cgatccagct
    64441 ttgggccaga taaggcagtt tcggatcgtt attgcgcaac gtcacgctga ttgatttgtc
    64501 gctttcatta aatatcaaac gggtacgatc tggcgttaca gaagcctggg cagacaaact
    64561 ggcagcgcta agcagtaacg ctagcgtcgt agcacccaat ttggcagagc aaagtaaatc
    64621 agacatggat cattccttaa tcacgttatt ggttagctga aacagaaaca gggggattgg
    64681 tgggtgtcac ctgttgcgtc tgctcctgaa tgacaggctt tatgtcatca ggaacaaccg
    64741 gcgcgacgtt gcctttatgc tggcatggca gtaacaagcc gttaaacaga tcggcaggca
    64801 gcgggtccgg caggttgata tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt
    64861 cgccaggttt caccccagcc agataaacat tgccgtcatc atcgacaagg cccactgtct
    64921 gctcgttatc attttttact tctgcgccaa acggaggatg gctgccgtct tgtaaacgca
    64981 gcaccgccat cgctttttga ccactgatga cggcaaattt gcggtagcca atcgcccctt
    65041 cagttagcgt ggcttgtacc accgactggg ttgcttcggc gttttccggc agtttgttga
    65101 ggtcgatata cgcctgattg cggtaatagt tattgacgtc agaaacgacg gctttaccaa
    65161 acatattggt ataaacagca gcaccgttac cttctaccgg aacatcggca acgccatcgg
    65221 catcaatcaa caagcgtgtc ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg
    65281 cagtaagcgt tgcgccgccc tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt
    65341 agttcgcact gaggtcaacc tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc
    65401 tggtgtgttt gtcactggtg ccgacgttca actgatagtg agtcgcgtca tcgacacggc
    65461 tgaaataacc gacctgactg ctgtccgtcc cactgccata gtaccgttat agctaacggt
    65521 actgttgtcg ccccacggca tactgagcga aatgtacatg cctttgtcgg cctggttgtc
    65581 atactcgtaa cggtagccag tcatcgagat gctgacatta cgaatactgc ccatattgaa
    65641 atagtgcgag agcatgatgt tgtagtttgt ctgctcctcg cgatcccagt aggtatggcg
    65701 ggtatagttg agataaaccg aaacacccgc atcgcggaag ttctggttat aagtggcggt
    65761 gtacatctct ttgtcgttgc ccgtgcgtac cattccgctg tcactggcat ccagatactc
    65821 gctcatggtc ataaagttct cttccgagaa gcgatatcca gcaaaagtaa cgcggctgtt
    65881 gagctggtca aagtctttgg aataactcac acggaaggag ttaccgtcca gcgaaccttt
    65941 gccataagcg gtgtctttat ccagtttggt gtgcgagtga gtaacatcaa acgcgaccgc
    66001 gccgaatgta gacaaatcgc gaccgacacc aagcgccgca gactgatagt tttcatctcc
    66061 cagtgcgccg ccatataacg accagccgtt agcgatcccc caggaagctt cagcaccaga
    66121 aaaaaattca ccctcgacat ggtgtcccca ctcttgcgga cggcccatca tgatcttata
    66181 gcgaacctga cctggacgag tgaggtatgg catcgaggcg gtgctgatgt catattcctg
    66241 cacctggccg ttctgttctt caatgcgaat atgcaacgta ccggagacag aatcaccaag
    66301 atcctgaata cgaaacggtc cagccggaac ctgcgtttcg taaatcacac gccccatctg
    66361 gctgacggtc acttttgctg tggtgtgtgc cacgccggaa atgtctggcg cgtagccgcg
    66421 cagattggga ggcaacattt gatcgtcagt actgacactg ccaccaacat agttaaaacc
    66481 atcaaaaata tcggatctga ggtaatcctc gcccagcgcc agtttggctt ttaatgatgg
    66541 taacgcccgc ctggcatagt agcgactcca ctcccatttt ttttgagttt catcgccgct
    66601 gaattcatcg tcatcattac tgcgagtatg ttgatagtta gtctgccagt cagcacgcat
    66661 acgccacggc cctaggttaa ccccaaccgt cccgttgccg ctgatctcgt tactatcatc
    66721 accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga cgatcccgga
    66781 gatgccgtca tcccaacgtg aaggcggatc ccagtcggga taagtatatt cgaggtaagc
    66841 ctgtggcagt gaaatgacta atgcggactg gcttaaatca gccttaattt ccatgccttc
    66901 cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt tcgccacgtc
    66961 ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc ttttgctcgc
    67021 gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct gtttatttag
    67081 ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa aacgcttcag
    67141 atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc
    67201 agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg gaagaatccg
    67261 taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt cttgttcggt
    67321 caaaatccat ttaaatcccc catagcagtg tcgctatggg ggtatgcctt acaggtaagt
    67381 aatctggaag gtggtatttg cttcaaaatt acccagatct ggcgcatcag ctgcgccgac
    67441 cagccaggct ttaaagttca gtgtctgctt cgctttgcct ttgttggtag aagtgtcttt
    67501 cactattttc tggtcaatac ccatgccgct tttgtaagag gtacccagag agtcaccgat
    67561 cgccaggcta acattgttaa atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt
    67621 gccgctattt gcagaagaaa cggtaccagt aaaggtagtg gtcattgttt cctgagtatc
    67681 aaatacgcaa tcttgcagac gaatctggaa acttttcggc gtggtggtgc cgttattttt
    67741 cagtttttcg gcaccgatat cacccatatc aacttccagt ttgtgatcgt ccggaacgat
    67801 agaacacact gagtcttcaa tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc
    67861 gattgttgcc gtagaggtga acacgcccat caccattgcc gcagcaatag ctgttttaac
    67921 aaacttactc attatttgtc cttaaactat aaataattaa aaatataaac attgcaatac
    67981 attgattcag tcaatagcca atgtttatac ccagccattg aatacggcat gcgacccaaa
    68041 aagaacaggt cgtagaaaat tgtattcatt ggtgtggggc cgcatagtga tgtttccaca
    68101 cccctgatcc aatttaattt tactaaagag taaatgtaga tttaattaat atattgacag
    68161 aagaaaggtt atcttaagga catccttaaa aaatacggaa taagtattta gaaaaaatag
    68221 aaagtatatc aataatgaat atcaacagct taaaaaacaa acaatgaaca tataatgcga
    68281 ttggcattta tccacttttg acaatataat atataaacac tatagccaga atttaatcaa
    68341 gtttcaatta catcctgcct gattattcaa ctctattttc tctattatct gtattaataa
    68401 atgacactga gtgtcaaaca gacattattc atttcaacat gtaactttgg gcaaaatcaa
    68461 gatacgcgca gaagaataat ttccgaatga atgtagaaag tgttttattt atagataagg
    68521 agagtcttcg atagtagtga ggttgacaga tatccgctgc ctaatgagca attatgccca
    68581 atgaatctac ctcattgggc ataatttgga acacatcaga tagcttttgc catcttcaga
    68641 ttacacgggc tcatctgcca gttgaatgta ccgtttccgc tttcatcaag cgttacgctg
    68701 gcaatggccg aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga
    68761 tacccgacta acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac
    68821 gcctgcaaat aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact
    68881 tctgcactgg aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc
    68941 aggaacgggc tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc
    69001 atcaggcgag attcgtcaca accattagtg gtcagaggac gaaccgaatc actggcggca
    69061 tcgagggctg cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa
    69121 ccagattcgc ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt
    69181 gccttatcca ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa
    69241 tatgcgttta cattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc
    69301 ttcttattgc aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc
    69361 gaccaaacgc tcgcaagggg taaaacggga accatactgc gtggccagtc gttgcattat
    69421 tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg
    69481 cggaaaacca atgccaaata ccgcaccaat atccccgtca cgcacgctac ggataacctg
    69541 ctcatcaaca caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg
    69601 cggtgcggag agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg caggatcgac
    69661 ctgttttttg cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct
    69721 gcctttgcga tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc
    69781 tccataagcg gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc
    69841 caaaagttgg attgggccta ccggaaaacc aaatttcact agcgcggcat caatgtgctc
    69901 tacccgttca ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg
    69961 attgacgtaa aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc
    70021 cagttttacc gtagtggcga tggtttgcgc cgatgtaccc gcatgaggaa taatctctac
    70081 cagcggcatt ttttccaccg gactgaagaa atgcaggcca ataacttgct caggtcgcgc
    70141 ggcgtgagcg gcgatatcac caatcggtaa agatgacgtg ttcgaagcaa aaatggtatg
    70201 agcggcgcaa ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa
    70261 caccgcttca ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat
    70321 taatgccagc tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc
    70381 cagctgatcc caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat
    70441 tctgaccgga agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc
    70501 accacctaaa attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt
    70561 cttcacgtcc gtactggcaa aaaagatact acgcaaagct tgcgattgcg gcgtcatcgc
    70621 cagttcgcca aaggcgcgag cttcagcgtc atagccgctg ctggtgccct gcgctaatcc
    70681 cgtttcaaca acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc
    70741 tgttttcttg ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg
    70801 ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcggc
    70861 ttccagcaaa atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt
    70921 cgcccgaagt tgttttccgg tgagaatcat ctctaatgct gtactgacgc ctatcagacg
    70981 cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc
    71041 gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccagctccag
    71101 cccaccgccc aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat
    71161 ctccgccatc aactgttggc cctgccgcgc cagtgcttcc gcttcttgcg ccgttttgca
    71221 gttgccaatc atgttgatgt ctgctccggc gataaaattg tccggtttcg cggagataaa
    71281 caccacgccg cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga
    71341 ggcaaattcc gccttcaggg tattcatttt ctcatccggt acgtcgatgg tgatgatagc
    71401 aatgttgtcc agacgaacat taagagtaaa cgctgatgcc atttccatta ttccgcctcc
    71461 agaaccattg ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg
    71521 ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc gcgcgccggt cgccgcgaag
    71581 ggatgtccgt aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca
    71641 gtggcatgtg cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata
    71701 ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag ttcgctcatc
    71761 gtcaaaccgg cacgttccag cgccagcggt gttgaccagg ctggaccgag caacatgtcc
    71821 tgccagacat caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct
    71881 aattctttcg cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc
    71941 gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg
    72001 taatcggcaa gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa
    72061 ggagggataa aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgagcg
    72121 gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt
    72181 ttcgccattt gttcggcggt gtcgcccatc cgcaagccag tagaatattc tgctaccgca
    72241 ggcggtacgg gcattaagtc gcgcaaacgc aggcgagaga agagtttcag gcgctggctc
    72301 atggttcgag ctttgttgac atcaaccagc acgtgcgcca gttttttact gacgccaatt
    72361 ggcaatacgg aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc
    72421 aggctttctg cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg
    72481 taagcatcgg tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg
    72541 gcttcaggca tttgtacgac ctgaccaaag accagctgtt caatcacttc agcggggatc
    72601 tcgctgcgtg ccagcagttc gcctaccacc atcttcccta aatcaaccgc gggaatgcca
    72661 tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga
    72721 tcgccctggc gggtaaccag tggtaaaacc tgacccataa cactcccctg taaaaaaata
    72781 aataaagtgg tctgacctga tcatagtatt aaccattttt ttacatttag ccaagcggag
    72841 aaaaaggaaa gtgggagcta tgacacagtg aagacagaag aaagaaaaga aaacgcccct
    72901 gccagcaact gacaggggcg tacgtacccg agaggaatta acgcagaccc agctggaaga
    72961 tcagcatttc agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact
    73021 cttcagtgaa agtcggggag attttgcacg gttcggattc cacgctacgg gatttttcag
    73081 tcagcgccgc cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg
    73141 cggtgcagtc ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt
    73201 catcagcact acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat
    73261 aaacgttgtg gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa
    73321 tcggataagt gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg
    73381 cgagtgcgca acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa
    73441 aagatggaaa catacttgca acattctagc tggtccgacc tatactctcg ccactggtct
    73501 gatttctaag atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag
    73561 tttgtataaa aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag
    73621 tctgctctcg cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag
    73681 ttaaacgaat tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc
    73741 gatgatgcag gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca
    73801 ttttctgcgg gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct
    73861 ggtcgtagcc tgcaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt
    73921 gttgcaccga ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg
    73981 gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa
    74041 accatgaacc tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt
    74101 ggtttcaacg ccatctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag
    74161 ttggttgctg gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg
    74221 gcagctaccg ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag
    74281 tggggctttg gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca
    74341 ctgacctacc gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat
    74401 cgtgcgttta ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg
    74461 ggttatctga cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat
    74521 ccacagtggg cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg
    74581 aaagcgacct caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct
    74641 taccgcatcg cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt
    74701 atcgcctttg atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag
    74761 gaccgtttct ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac
    74821 gttggtgttt cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc
    74881 gagtctgaag gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc
    74941 gttcgcctgg ataaagtcac ctgctatgca ggtgacttta actcccccac ttcaccgaaa
    75001 gtagtttcct cattgcttgc ctcctgagtt ttgcaaacag cctgatggca gcttgccctt
    75061 tcaaatcagt aagtggtatc cacagacaaa cctgaaggga aatgcatatt ttcaagcgtt
    75121 ctgctcgtcc ttctcaaaga gtttactttt ctgcatttcc aggatactca acccccctgg
    75181 ctattgtgcg cttatacact caaattaaag ataggttcta aataaatgag cgttttttga
    75241 tagtctattt cattaggtaa tatatatttg taacaaatca atcaacatag aataaaatca
    75301 tgctaccatc tatttcaatc aacaatacca gcgcagctta cccagaatcc atcaatgaaa
    75361 ataacaatga tgaaattaat ggattagtac aagagttcaa aaaccttttt aatggtaagg
    75421 aaggaataag cacctgtatt aaacatctac ttgagcttat aaaaaacgcc atacgagtaa
    75481 acgacgatcc ttatagattt aatattaata attcctcagt tacttatatt gatattggct
    75541 ccaatgatac agaccatatt actattggta tcgacaacca atagaattac ctgcgaacta
    75601 taaagacaaa gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga
    75661 tatcaataac aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg
    75721 ttttatcttc gataagatat cacacaaact cagacatacg gtaacggaat ttgatgagag
    75781 cgggaaaagc gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcgactc
    75841 tctcgctatt gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga
    75901 tcaggacgac taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata
    75961 ttgtagtgaa aacgccagga gtgcttcagc gtggtttgaa agcagtaaag ctatcatggc
    76021 agaatcattt gcaactggtt ccgatcatca ggttgtaaac gagctcaacg gggaaagact
    76081 gagagaacct aacgaagttt ttaaacgttt aggtcgagca ataagatata attttcaagt
    76141 ggacgatgca aaatttagac gcgataatgt aaaagaaata atttctactt tattcgctaa
    76201 caaagttgac gttgaccatc ctgaaaataa atacaaagac tttaaggatc tcgaagataa
    76261 agttgaaaaa agacttcaaa attgccaggc gaaatatcaa aatgaaatta atcaactatc
    76321 tgcactaggt gttaattttg atgatattta aaattatttt tccagttttt tgatttttca
    76381 acgcctgaat atcggaaata gtcagtgaca aa
//
LOCUS       DADLXX010000004        69006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLXX010000004 DADLXX010000000
VERSION     DADLXX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.004"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..188
                     /locus_tag="JNX27_000248"
     CDS             <1..188
                     /locus_tag="JNX27_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7881589.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNX27_000249"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNX27_000249"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7881590.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /locus_tag="JNX27_000250"
     CDS             1156..3192
                     /locus_tag="JNX27_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7881591.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="JNX27_000251"
     CDS             3150..3545
                     /locus_tag="JNX27_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7881592.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="JNX27_000252"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="JNX27_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7881593.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="JNX27_000253"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="JNX27_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7881594.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="JNX27_000254"
     CDS             complement(4854..5894)
                     /locus_tag="JNX27_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7881595.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="JNX27_000255"
     CDS             complement(5967..6602)
                     /locus_tag="JNX27_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7881596.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="JNX27_000256"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="JNX27_000256"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7881597.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTSAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="JNX27_000257"
     CDS             complement(7228..7671)
                     /locus_tag="JNX27_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY7881598.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNX27_000258"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNX27_000258"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7881599.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGVAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="JNX27_000259"
     CDS             complement(8011..8514)
                     /locus_tag="JNX27_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7881600.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="JNX27_000260"
     CDS             complement(8508..9032)
                     /locus_tag="JNX27_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7881601.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="JNX27_000261"
     CDS             9241..10236
                     /locus_tag="JNX27_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7881602.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="JNX27_000262"
     CDS             10245..11123
                     /locus_tag="JNX27_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7881603.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="JNX27_000263"
     CDS             11204..12211
                     /locus_tag="JNX27_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY7881604.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSIQQAFVKLRRGEIRQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNX27_000264"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNX27_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7881605.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /locus_tag="JNX27_000265"
     CDS             complement(13727..15616)
                     /locus_tag="JNX27_000265"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7881606.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNX27_000266"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNX27_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7881607.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNX27_000267"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNX27_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7881608.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNX27_000268"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNX27_000268"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7881609.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNX27_000269"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNX27_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7881610.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNX27_000270"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNX27_000270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7881611.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNX27_000271"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNX27_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7881612.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNX27_000272"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNX27_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7881613.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNX27_000273"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNX27_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7881614.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNX27_000274"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNX27_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7881615.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25945..26021)
                     /locus_tag="JNX27_000275"
     tRNA            complement(25945..26021)
                     /locus_tag="JNX27_000275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25985..25987),aa:Met,seq:cat)
     gene            26369..27712
                     /gene="argG"
                     /locus_tag="JNX27_000276"
     CDS             26369..27712
                     /gene="argG"
                     /locus_tag="JNX27_000276"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7881616.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27720..29327)
                     /locus_tag="JNX27_000277"
                     /pseudo
     CDS             complement(27720..29327)
                     /locus_tag="JNX27_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29805..29891)
                     /locus_tag="JNX27_000278"
     tRNA            complement(29805..29891)
                     /locus_tag="JNX27_000278"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29855..29857),aa:Leu,seq:gag)
     gene            complement(29906..30238)
                     /gene="secG"
                     /locus_tag="JNX27_000279"
     CDS             complement(29906..30238)
                     /gene="secG"
                     /locus_tag="JNX27_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7881617.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSTPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="JNX27_000280"
     CDS             complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="JNX27_000280"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7881618.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31796..32644)
                     /gene="folP"
                     /locus_tag="JNX27_000281"
     CDS             complement(31796..32644)
                     /gene="folP"
                     /locus_tag="JNX27_000281"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7881619.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="JNX27_000282"
     CDS             complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="JNX27_000282"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7881620.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="JNX27_000283"
     CDS             complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="JNX27_000283"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7881621.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35523..35816
                     /gene="yhbY"
                     /locus_tag="JNX27_000284"
     CDS             35523..35816
                     /gene="yhbY"
                     /locus_tag="JNX27_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7881622.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35972..36448)
                     /gene="greA"
                     /locus_tag="JNX27_000285"
     CDS             complement(35972..36448)
                     /gene="greA"
                     /locus_tag="JNX27_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7881623.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36696..38129
                     /gene="dacB"
                     /locus_tag="JNX27_000286"
     CDS             36696..38129
                     /gene="dacB"
                     /locus_tag="JNX27_000286"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7881624.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="JNX27_000287"
     CDS             complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="JNX27_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7881625.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39503..40468)
                     /locus_tag="JNX27_000288"
     CDS             complement(39503..40468)
                     /locus_tag="JNX27_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7881626.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLSPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="JNX27_000289"
     CDS             complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="JNX27_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7881627.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="JNX27_000290"
     CDS             complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="JNX27_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7881628.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41443..42414
                     /gene="ispB"
                     /locus_tag="JNX27_000291"
     CDS             41443..42414
                     /gene="ispB"
                     /locus_tag="JNX27_000291"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7881629.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42642..42920
                     /gene="sfsB"
                     /locus_tag="JNX27_000292"
     CDS             42642..42920
                     /gene="sfsB"
                     /locus_tag="JNX27_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7881630.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(42968..44227)
                     /gene="murA"
                     /locus_tag="JNX27_000293"
     CDS             complement(42968..44227)
                     /gene="murA"
                     /locus_tag="JNX27_000293"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7881631.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="JNX27_000294"
     CDS             complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="JNX27_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7881632.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="JNX27_000295"
     CDS             complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="JNX27_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7881633.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="JNX27_000296"
     CDS             complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="JNX27_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7881634.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="JNX27_000297"
     CDS             complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="JNX27_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7881635.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="JNX27_000298"
     CDS             complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="JNX27_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7881636.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHVPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="JNX27_000299"
     CDS             complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="JNX27_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7881637.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48008..48973
                     /locus_tag="JNX27_000300"
     CDS             48008..48973
                     /locus_tag="JNX27_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7881638.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLLLGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48987..49973
                     /gene="kdsD"
                     /locus_tag="JNX27_000301"
     CDS             48987..49973
                     /gene="kdsD"
                     /locus_tag="JNX27_000301"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7881639.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49994..50560
                     /gene="kdsC"
                     /locus_tag="JNX27_000302"
     CDS             49994..50560
                     /gene="kdsC"
                     /locus_tag="JNX27_000302"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7881640.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50557..51132
                     /gene="lptC"
                     /locus_tag="JNX27_000303"
     CDS             50557..51132
                     /gene="lptC"
                     /locus_tag="JNX27_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7881641.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51101..51658
                     /gene="lptA"
                     /locus_tag="JNX27_000304"
     CDS             51101..51658
                     /gene="lptA"
                     /locus_tag="JNX27_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7881642.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51665..52390
                     /gene="lptB"
                     /locus_tag="JNX27_000305"
     CDS             51665..52390
                     /gene="lptB"
                     /locus_tag="JNX27_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7881643.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52438..53871
                     /gene="rpoN"
                     /locus_tag="JNX27_000306"
     CDS             52438..53871
                     /gene="rpoN"
                     /locus_tag="JNX27_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7881644.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSRLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53894..54181
                     /gene="hpf"
                     /locus_tag="JNX27_000307"
     CDS             53894..54181
                     /gene="hpf"
                     /locus_tag="JNX27_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7881645.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54299..54790
                     /gene="ptsN"
                     /locus_tag="JNX27_000308"
     CDS             54299..54790
                     /gene="ptsN"
                     /locus_tag="JNX27_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7881646.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54836..55690
                     /gene="rapZ"
                     /locus_tag="JNX27_000309"
     CDS             54836..55690
                     /gene="rapZ"
                     /locus_tag="JNX27_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7881647.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55687..55959
                     /gene="npr"
                     /locus_tag="JNX27_000310"
     CDS             55687..55959
                     /gene="npr"
                     /locus_tag="JNX27_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7881648.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56172..56804
                     /gene="yrbL"
                     /locus_tag="JNX27_000311"
     CDS             56172..56804
                     /gene="yrbL"
                     /locus_tag="JNX27_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7881649.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="JNX27_000312"
     CDS             complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="JNX27_000312"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY7881650.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="JNX27_000313"
     CDS             complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="JNX27_000313"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7881651.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="JNX27_000314"
     CDS             complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="JNX27_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7881652.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60841..61770)
                     /locus_tag="JNX27_000315"
     CDS             complement(60841..61770)
                     /locus_tag="JNX27_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7881653.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62445..66905
                     /gene="gltB"
                     /locus_tag="JNX27_000316"
     CDS             62445..66905
                     /gene="gltB"
                     /locus_tag="JNX27_000316"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7881654.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66918..68336
                     /locus_tag="JNX27_000317"
     CDS             66918..68336
                     /locus_tag="JNX27_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7881655.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68520..68999
                     /locus_tag="JNX27_000318"
     CDS             68520..68999
                     /locus_tag="JNX27_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7881656.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16847 a        18748 c        17156 g        16255 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aattagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aacctgtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgtcagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca ggggttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatac agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaatcaggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aataatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcagcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    25981 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    26041 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    26101 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    26161 gtgaatgaat atccagttca ctttcatttg ttaaatactt ttgccttccc ctgctctccc
    26221 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    26281 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    26341 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    26401 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    26461 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga
    26521 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    26581 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    26641 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    26701 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    26761 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    26821 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    26881 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    26941 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    27001 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    27061 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg
    27121 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    27181 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    27241 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    27301 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    27361 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    27421 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    27481 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    27541 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    27601 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    27661 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    27721 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    27781 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    27841 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    27901 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    27961 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    28021 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    28081 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    28141 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    28201 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    28261 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    28321 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    28381 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    28441 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    28501 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    28561 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    28621 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    28681 atacttgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    28741 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    28801 gcaaatcacg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    28861 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    28921 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    28981 aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttcac aacttcatcc
    29041 ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    29101 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    29161 agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag gacgaagaac
    29221 agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat accaaaaaca
    29281 ataacacaaa gatacaacaa ccaatgagat ttaaaatctt ctagcaaatt tgttgaatac
    29341 tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat gttcaatttt
    29401 atatcagaca tgtttttcaa ctctgaacca tatcagaccg aaacactgtt agttttttct
    29461 ttttaaatga tgcgaccatt gcgttctcag taattaaata acattatcaa tgcgtctcta
    29521 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
    29581 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
    29641 gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt caaggtacca
    29701 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcat cagaccagga
    29761 ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
    29821 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    29881 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    29941 cagctggctg agtttgttcg gttttcgccg gtgtactcag attttcccat tcgctacctc
    30001 tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    30061 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    30121 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    30181 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    30241 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    30301 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
    30361 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
    30421 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    30481 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
    30541 cacgcgaatt aacggttcgg tgccggattt acgcagcaac acgcgtccac ggttgcccag
    30601 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg gatcgccgct
    30661 acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    30721 gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat
    30781 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct ctgcaccgat
    30841 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
    30901 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    30961 cacagcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    31021 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
    31081 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
    31141 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
    31201 aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
    31261 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    31321 aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca gttctgccga
    31381 atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg
    31441 cagtttagtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg ggttatgcga
    31501 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    31561 tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag
    31621 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
    31681 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
    31741 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    31801 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    31861 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
    31921 aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
    31981 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    32041 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    32101 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
    32161 tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct
    32221 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
    32281 cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga
    32341 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    32401 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg
    32461 tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt
    32521 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
    32581 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
    32641 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
    32701 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    32761 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
    32821 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    32881 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    32941 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    33001 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
    33061 atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag
    33121 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
    33181 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
    33241 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    33301 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    33361 aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    33421 cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    33481 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    33541 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    33601 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
    33661 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac
    33721 ctggcggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt
    33781 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    33841 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    33901 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    33961 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
    34021 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc
    34081 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    34141 gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca
    34201 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    34261 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    34321 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    34381 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    34441 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    34501 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    34561 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    34621 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    34681 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    34741 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    34801 ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    34861 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    34921 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
    34981 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    35041 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    35101 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    35161 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
    35221 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    35281 aagtttaaac caggcacggg aacgtaatcc ctttttctgt gcctgttgaa catatttatc
    35341 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    35401 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    35461 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    35521 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    35581 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    35641 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaacaaaaa
    35701 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    35761 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    35821 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    35881 aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg gcctttttcc tttctttaca
    35941 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    36001 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    36061 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    36121 cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag
    36181 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgtttg
    36241 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
    36301 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    36361 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    36421 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    36481 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    36541 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatggt
    36601 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    36661 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    36721 gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc
    36781 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggtgcg tcggcccccg
    36841 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa gtgattactg
    36901 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
    36961 aaggcaatgt ggaaaacggc atacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    37021 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    37081 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    37141 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgct gccgccatag
    37201 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaagcctggt gatatggctt
    37261 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    37321 gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaggcgac ctgaaccgct
    37381 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttggcgtgc
    37441 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcgggtatca
    37501 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
    37561 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    37621 tgattgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
    37681 ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    37741 gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    37801 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
    37861 tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    37921 gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    37981 gattcattac cacagcgagc gggcaacgaa tggcatttgt acaatatctt tctggctatg
    38041 cagtaaaacc cgcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    38101 tgtacaaaga tatttatcag aacaattagt caaaaagaaa ccccggcgca tggccggggt
    38161 ttcagattat tgacaaagtg cgctttgttt atgccggatg cggcgtgaac gccttatccg
    38221 gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct tgtaggcctg ataagtgtag
    38281 cgcatcaggc ttatttggca ttggtcatta gtgattaacg cttgtaaatg aactcaacgc
    38341 cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc atcatcctct tcagcaatct
    38401 cttcaagctg ctgacggtga taatcatccc acatgaattc gactttctct ggctgtttcg
    38461 cttcttcagc ctgcacgacc gggttttcaa tgataaaggt catcacatcc cagcagagat
    38521 ctttcacgcc cagtccgctc gccgcagaga tcagataata tttatcctcc cagcccagcg
    38581 cttcagcgat cgctttcgct ttctcttcgg cttctgcctt atccagcaga tcgatcttgt
    38641 tgaagactaa ccaacgcggt ttcgccgcca gatcctggct gtatttttcc agctcgctga
    38701 taataatacg cgcgttttca accggatcgg tgccgtcaat cggatcgata tcgatgaggt
    38761 gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga cctgcgcctt
    38821 ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac gaagctcttt tcgttgtcca
    38881 ttcgtaccac acccagactt ggcaccagag tggtaaacgg ataatccgcc actttcggtt
    38941 tagccgccga taccgcacga ataaaggtcg atttacccgc gtttggcatc cccaacatac
    39001 cgacgtcagc cagcagcatc agctccagca gcagctcacg cttatcgccc ggcgtaccgt
    39061 tagttttctg ccgtggtgta cggttaactg acgatttgaa acgggtattg cccagaccgt
    39121 gccagccgcc cttagcaacc agcagacgct gaccgtgttt agtcatatca cccatggttt
    39181 caccagtacc ctggtcgatt acacgcgtac ctaccggcac tttaatcgtc acgtctttac
    39241 cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc gcgctctgca cggaaagatt
    39301 tttcaaaacg ataatcgata agcgtgttca ggttctcgtc ggcttccatc catacgtcac
    39361 caccatcacc gccgtcgccg ccatccgggc cgcctttcgg aatatacttt tcgcggcgga
    39421 agctcacgca accattaccg ccatcacctg caacgaccag aatcgatgct tcatcaacaa
    39481 acttcatttt attctccgta aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat
    39541 gcttacgtaa tccgccccaa atacgatgac caatggcgga atacatcgcg cccgcaacca
    39601 cgacaaacgc accgagataa cctaaaaggt ttaacatcgg tctggcgaag aaatcaggcc
    39661 aggccagtga taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg
    39721 cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc caaggcgcca tatcctacca
    39781 aggtattcag tccgcaaaaa attaaacatg cgagctgcca gtggctaagc tgcgctatca
    39841 ctccaggctt tgccagaggg aagagcgcga ttgtacataa agtgtacagt aaaaacagga
    39901 tctgcggtga ggccagccga cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa
    39961 ccgtcgccgc accgacccca aagataactc cccaggtgta atcggtgagc tttgtaaata
    40021 tctcgaccag actggtgtta aaaaacatca ccagaccgct caggagcatc aatgccccta
    40081 caacctgagt gctgcgcatt ttctctttca ggataaatac gctggcaacc atcatgccaa
    40141 ctggcgagag ttgcccaatc acctgcgaag cggtcggact caggtattgc aaggatgagc
    40201 tgaacaggat gaagttccca aacagcccgg cggtcgccac tgccaacaaa atcaaccagc
    40261 gtggcttacg aaacacgcgt aatggcgaca acctcttctt caccgcaaga atggcaccaa
    40321 ggccaatact cgccatcaag aaacggtaaa acacgattgt cggaggttcc atcacctcca
    40381 gcacctgctt cattgcgatt ggcaacgccc cccagcaaat tgctgtggta agcgccaaaa
    40441 gaatgccaat gcctgcctgc tgcttcatac cgtattccct gcaaagcgca tttgcggatt
    40501 aacgtcgcac catgcgcatt tttaccggtt atcgaatgta aaaagccccg caacgtgttg
    40561 cggggctttc atccgttacc gggacgcgaa aaacttattc agcttcgatg ctgataaatt
    40621 tacggttttt cgggccttta acttcgaatt tcactttacc gtctgctttg gcaaacagag
    40681 tgtggtcacg accgcaacct acgttagcgc cagcgtggaa tttggtacca cgttgacgaa
    40741 cgatgatgct acccgccaga acggattcgc caccgaaacg cttaacgccc aggcgtttag
    40801 cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc
    40861 tctcctcagg tcttaggcgc tgatgccagt aattttcaca tcagtgaacc actgacgatg
    40921 gccctgctgc ttacgatagt gtttacgacg acgaaactta acgattttaa ctttctcgcc
    40981 acgaccgtga gcaacaactt cagctttgat tacgccgcca tcaacgaaag gaacgccgat
    41041 tttgacttct tcaccgtttg cgatcatcag cacttcagcg aactcaacag tttcgccagt
    41101 tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg cttactcggt gttgtttacc
    41161 accactttgg aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct tttcaatgat
    41221 tcagagtgcg ctataaatat tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt
    41281 gacaagtgct acagtcaata cacgaagaaa aaaaacacaa cttgtacggt aacgtttatc
    41341 tgtgccattt tttcagtaca attaccctat attcctaacc ataaacccta agttgccttt
    41401 gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc
    41461 aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg caatccttga gcagcttaat
    41521 tccgacgtcc aactgatcaa tcagttaggc tattacatcg tcagcggcgg cggtaaacgt
    41581 attcgtccga tgattgctgt actggctgca cgagctgttg gctatgaggg aaatgcgcat
    41641 gtcaccattg ctgccctgat cgagtttatc cacacggcga ctctgctaca cgacgacgtt
    41701 gtggatgaat cagatatgcg caggggtaaa gctaccgcca acgccgcatt tggcaatgcc
    41761 gccagcgtgc tggtaggcga ttttatttat acccgcgcgt tccagatgat gaccagcctc
    41821 ggttcactca aagtgctgga agtcatgtca gaagctgtaa acgtcatcgc agaaggtgaa
    41881 gttctgcaac tgatgaacgt taacgatccg gacatcactg aagaaaacta catgcgcgtt
    41941 atctatagca aaaccgcgcg tctgtttgag gctgccgcgc agtgttccgg gattctggct
    42001 ggctgtacgc cggaggagga gaaaggcctg caggattatg ggcgctatct cggcactgct
    42061 ttccagttga tcgacgattt actcgattac aatgccgatg gcgaacagtt aggtaaaaac
    42121 gtcggcgacg atctgaacga aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat
    42181 ggcacaccag aacaggcaca gatgatccgt accgccatcg aacaaggtaa cggtcgccat
    42241 cttctggaac cggttctgga agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag
    42301 cgcgccgagg aagaagcaga caaagccatc gcagcgttac aggtgctccc ggacacccct
    42361 tggcgagaag cactcatcgg cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct
    42421 cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat tccataaaaa cactaattca
    42481 gcgctataac acctgaaaaa aagggcgatg aatctttata tattctgaat attcacactc
    42541 tttacaggaa ctttttagcg caataggcca tcaggagtat agtgatgccc gacagaagaa
    42601 gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa gatggaaagt aatttcattg
    42661 actggcatcc cgctgacatc attgcaggtt tgcgcaagaa aggaacgtca atggcggcgg
    42721 aatctcgcag aaatggtttg agttcctcaa cgctggcaaa cgcattatcg cgcccatggc
    42781 cgaaaggaga gatgattatt gcgaaagccc tgggaactga cccctgggtt atctggccat
    42841 cacgctacca tgatccgcgg acccatgagt ttatcgacag aacgcagttg atgcgtagct
    42901 acactaaacc gaaaaaatga gtggtctggc ggtagccccg cgaacggggc tgccagctct
    42961 cagacgatta ctcgcctttc acacgctcaa tatttgcacc taaagcgcgc agtttgtctt
    43021 caatgcgttc gtagccacga tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg
    43081 caatacagcc agccagcacc aggcttgctg atgcacgcag atcggttgcc ataacctgtg
    43141 caccagaaag tttttcaaca ccatgacaaa taacggtatt gctttcgatt tcggcgtgcg
    43201 cgcccatacg gctcagctct ggcacatgca taaagcggtt ttcaaagacc gtttcggtga
    43261 tgaacccggt cccttcagcc accaggttca acagcgtgaa ctgggcctgc atatcggtcg
    43321 ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt cggacgtttg ccatgcatat
    43381 ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc agcatcacgc agtttcgcca
    43441 gcacggcgtc cagagtatct ggctgcgcgt tacggcagat aattttgcca cgagagatcg
    43501 ccgccgccac caggaaagta ccggtttcga tacgatccgg cagcacgcga tagacaccgc
    43561 cgcctaaacg ttccacacct tcgatgacga tacgatcggt gccctgaccg ctaattttcg
    43621 cacccagcgt aatcaggaag ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt
    43681 caataatcgt ggtgccttcc gccagggttg cagcacacat gatggtcacc gttgcgccaa
    43741 cgctgacttt atccatcacg atatgtgcgc ctttcagacg accatcgacg gaagctttaa
    43801 cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc gaggccagaa atgtgtagat
    43861 caaccggacg agcaccgatc gtacaaccgc caggtagtga aacttgcccc tgaccaaagc
    43921 gcgctaccag cggccccagc gcccagatag aagcacgcat ggttttaacc agatcgtaag
    43981 gtgcgcagaa tacattaacg tcgcgggcat caatatgcac agaaccatta cgttctactt
    44041 tcgcacccag ctggcttagc agtttcattg atgtatcgac gtctttcagt ttcgggacgt
    44101 tctggatctc taccggttct tccgccagta gtgcggcaaa aagaataggc agagcagcat
    44161 ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt tggcccctga acacgaaatt
    44221 tatccattta gtttgttctc agttaacaat tcatatccgc taccggcgaa tcgcccatag
    44281 ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc aggggtatac gctttgatcg
    44341 acacagcatg aatgcggtta tccgcaatat attccatcag cgggccatag accgtctgct
    44401 gttttttaac ccgactcata ccgtcaaaca actcacccac ggcaataacc tgaaagtgac
    44461 tgccatcgcc ggaaacgtgg acttcctgga gggagagagc gttcatcaac acgctctgaa
    44521 tttcattatt ttccatggga tcttcaatca tcagttaata aaccagcgaa acatcttaga
    44581 gcaaagttgc gctggcataa ataagcaaaa agcctcgctg ataaatcaga caaggctcga
    44641 cttgcaggca ggtttgccgg acaggcggtt aacgccatat ccggcctgaa aaaatttaac
    44701 gaggcagaac atcagcaggc aaattataca atttcgcgag ggtatacact ttgtcgttta
    44761 ccccctgaag cgtcacgttg ttgccctgct ttttcgccag atcgataaga tggagcagca
    44821 gtgccagtcc ccccgtatcc acgcgggaca cacggctaag atcgatgcag gtaatcccct
    44881 tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac gtcctgatcc agctctccgg
    44941 ataacgccag cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt attttttctc
    45001 ttccagagtg attttctgtt gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc
    45061 tttggtacgc agcagcgttc cccactcgtt ttgtttggtg gtgatcatac tgacgccttc
    45121 agcaatcatg tcgtaagctt gccagtttcc cgtctgggag tttttacgcc actggaagtc
    45181 cagacgcacc ggcggacggc cattcgggtc aataatggta acgcgaatag gcacaatggt
    45241 tttatcgccc agcggctgtt ctggcgcaat ctgataggtt tgaccgtgat acatcgccag
    45301 cgcctgaccg taagcctgct tcaggtactc acggaaagcg gcaaagtagg cttcacgttg
    45361 agcaggggtc gcactcttgt aatactggcc cagcaccagc gcaccggcgt atttcacctg
    45421 tacgtatggc agcagttcct gatcaacaat ggtacgcaga taatccgggt tggcccgaat
    45481 ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt
    45541 ataaggattg gtctggtctg ccgcggttgc cgcactcaga ggtgcaatca ccagcaaagc
    45601 gaccatcatt aaacgtttaa acatgcgtcg gttctcctga aattatttcg ttgtacccac
    45661 aggttcagtg gtttcattat tacctggcgc agcagctggc gcatcgccac tattcttatt
    45721 gtcatcgcct ttactaccgt aaaggaactg accaatgaga tcttccagca ccatcgcaga
    45781 cttggtgtcc tgaattgtat cgccatcctt caggatagca gtccccagtt ccgggtcttc
    45841 aaaaccgacg tttaatgcca gatattgttc tcccagcagg ccggaagtac gaatgctcag
    45901 cgaactggta tcaggaatgt ggttataacg ttgttcaatt tccagcgtta cgcgcggcag
    45961 ataggttttc gggtccagcg taatatccgc cacccggccc acaacaacac caccaatgct
    46021 gaccggagag cgcgctttca ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt
    46081 cgattcagta cgtatggacg tcacgttcgc cgccttcagg caaacaaaca gcgccgccag
    46141 cagtgctgct aataaaaaga tacccaccca aatttcattt tttttcgttt gcatgaactc
    46201 aattcccaaa catcaatgcg gtcagcacaa aatccagccc cagaacagcc agagacgagt
    46261 ggacaacggt gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc
    46321 cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac cacgctctta atcagacagt
    46381 tgaccagatc catacgccag tcgacggcat tttgcattgc cgaccagaag aacccgctat
    46441 caatgccttt ccaactgacg ccgaccagcg atccgcccca gatccccacg gcgacgaaga
    46501 taaccgtcaa cagtggtaat gaaataaccc cagcccagaa acggggagaa ataacccgac
    46561 gcagcggatc caccgccatc atctccatac tggagagttg ctctgtagcg cgcatcaggc
    46621 cgatttctgc ggttaacgcc gaaccagcac gcccggcaaa caacagcgcg gcaaccaccg
    46681 gccccagttc acgcagtagc gataacgcca ccagcatacc cagactggtt tccgcgctat
    46741 aagtggtcag aaccagataa ccttgcagcc ccaacaccat tccgatgaac acgccagaaa
    46801 ctacaataat cagcatcgac aggacgccga cattatagag ctggcgcacc agcagcggca
    46861 catgtttgcg aaattccggt ttgccgacca gcgcattgaa taacattaac ccggcccgcc
    46921 cgaacgttct cagggtttta atccctttat gtccgagcga cgccagcgca tttaacagca
    46981 tgagtggctt aactccctgg taaaagatca gcgtgataat cgccggcagg atagcggaac
    47041 ggaacaggcc cgtcagctat cccgtccaga aactgacgta cgcgcggatc aggattcgtc
    47101 tgcaacgcct gggcactgcc atgagcgaca atttttttgt ccgccaggat ccaggcgtga
    47161 tccgcaatac ttaacacttc cggcacatcg tgagaaacca ccacacaagt cacgcccagc
    47221 gcgctgttca gctcagaaat cagcttcacc agtacgccca tggtgatggg atcttgccca
    47281 acaaaaggtt catcaaacat gatgagatcc ggctccagcg caatcgcacg cgccagcgct
    47341 gcacgccgcg ccatcccacc ggaaagttca gaaggcatta gtttagccgc tccacgcagc
    47401 cccacggcct ccagcttcat catcaccgta ctatgcaaca atggcgcggg aagttgggta
    47461 tgttcgcgca gtggataggc gacgttgtca aatacgttca tatcagtgaa caacgccccg
    47521 gactgaaata acatgctcat ccgtttacgc actgtataca ggcgcgaacg agacatcgcc
    47581 ggaatattct caccatcgaa aaggatctca ccatgatctg gtgcgatttg cccgccaatc
    47641 agacggagta gcgtcgtttt accgatgccc gatggcccca tgatcgccgt gatcttccct
    47701 cgcggcacgg tcagggaaat attatcgaag atgcagcgat tgccacgcgt aaaactgaca
    47761 tcgcgcatat cgactaaatt cgccacagac tgctccataa ttcacccttc gtcttgcgtt
    47821 gattttctaa gcatggcgct caatttaacc ttgaacccaa catatttaca gaatattacc
    47881 cgccgtggtt agcgaaagct ggcatttgtt ttacttttta gccgcataaa gtcaaaatta
    47941 agaatccgtt acggctttct gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg
    48001 acgttagatg cttttagcta cggcactgtt aattgttggt ttacttttgg tcgtttacag
    48061 tgccgaccgc ctggtttttg ccgcgtctat tctttgccga acctttggca tcccgccgct
    48121 gatcatcggc atgacggtgg tcagtattgg tacatcgtta ccagaaatca tcgtctcgct
    48181 tgccgcgtct ctgcacgaac aacgcgattt agccgttggt acagccctcg gctcaaacat
    48241 tatcaatata ttgctgctcc tcggtctggc tgcgctggtt cgtcctttta ccgtccattc
    48301 cgatgttctg cgccgtgaat tacccttaat gttgttggtc agcgtggtgg ccggttccgt
    48361 actctatgac ggacaactta gtcgaagcga tggtatcttt ctcctctttc tggctgtgct
    48421 atggctgctg ttcattgtta aacttgcacg tcaggggact gacagcctga ccagagagca
    48481 gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc
    48541 gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa
    48601 ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagtct
    48661 gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg
    48721 aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat
    48781 aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt
    48841 gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg
    48901 ggtattatta actggcggat ttatcatatg gctggcgatg ttgtactggt tatcgccaat
    48961 actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact
    49021 ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc
    49081 aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag
    49141 ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc gcaacgtttg
    49201 ccagcaccgg tacaccttca tttttcgtcc atcctagtga agccgcgcat ggtgatttag
    49261 gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa
    49321 tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc
    49381 gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag
    49441 aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg
    49501 atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct
    49561 cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata
    49621 cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag
    49681 ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca
    49741 tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa
    49801 gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg
    49861 aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc
    49921 atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca
    49981 aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag
    50041 cgccgacgtt atggcaaaag cagagaacat tcgtctgctg atcctcgacg ttgatggcgt
    50101 actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt
    50161 tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac
    50221 cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta
    50281 tcaggggcag tcaaacaaac tgatcgcctt tagcgatttg ctggaaaaat tggcgattgc
    50341 cccggaaaat gtggcttatg ttggcgatga tctcatcgac tgcccggtaa tggaaaaagt
    50401 gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt
    50461 gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc
    50521 gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt
    50581 atcattgtgc tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac
    50641 gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac
    50701 acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa
    50761 tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag
    50821 gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg
    50881 ctctatttat atggacacgt tgaagtcaac gcacttgtgc cagactctca acttcgcaga
    50941 atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc
    51001 gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc
    51061 agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa
    51121 actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc
    51181 cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat
    51241 gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat
    51301 taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga
    51361 cggatacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg
    51421 tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc
    51481 ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga
    51541 gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc tggtgccgtc
    51601 gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt
    51661 cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt
    51721 agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg
    51781 tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa
    51841 catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg
    51901 tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct
    51961 gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa
    52021 cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc
    52081 cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat
    52141 tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgata ttaaacgcat
    52201 cattgaacac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga
    52261 aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg
    52321 cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt
    52381 cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg
    52441 aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag
    52501 gcaattcgtc tgttgcagtt gtcgaggttg gaacttcagc aggagctaca gcaggcgctg
    52561 gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg
    52621 caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag
    52681 ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc
    52741 ggtgactaca ttgacgacga gctgccggtc taccagggcg aaacgacgca gaccttgcag
    52801 gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct
    52861 acctctatcg tcgatgccgt tgatgaaacc ggttatctga ctgtcccgct ggaagatatt
    52921 ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt ccttaagcgg
    52981 atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc
    53041 caactctccc aattcgataa gaccacgccg tggctggaag aggccagatt gatcattagc
    53101 gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg
    53161 aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg
    53221 cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat
    53281 aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag
    53341 cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt tatccgcagc
    53401 aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg
    53461 cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg tgaagaatat
    53521 atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata
    53581 tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat
    53641 ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt
    53701 gcactggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag
    53761 ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac
    53821 cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga
    53881 taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact
    53941 gcgcgaattt gttacagcta aatttgccaa acttgagcaa tattttgacc gaatcaacca
    54001 ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt
    54061 aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg
    54121 cttaattgat aagctggcac gccagttgac caaacataaa gataaactga aacaacacta
    54181 attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt
    54241 acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat
    54301 gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag
    54361 ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa
    54421 acaacttagc ctaccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg
    54481 cagtaccggt atcggcaatg gtattgccat tccgcatggc aaacttgaag aagatactct
    54541 gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa
    54601 ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct
    54661 gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg
    54721 cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga
    54781 tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt
    54841 actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga
    54901 agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg
    54961 aactctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga
    55021 gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact
    55081 actgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact
    55141 gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgattt
    55201 gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca
    55261 cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgaccat
    55321 ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga
    55381 cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga
    55441 taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac
    55501 gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta gctacctgac
    55561 ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc
    55621 agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg
    55681 taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg
    55741 cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat
    55801 gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc
    55861 aaaggacggc agattgaagt tgaagcgacc ggtccacagg aagaggaagc actggccgcc
    55921 gttatcgccc tctttaattc tggttttgat gaagattaat cttcatcact ttgacataca
    55981 actcccttca agctcccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac
    56041 gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa accttaagag
    56101 gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag aaaatatttc
    56161 caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga
    56221 aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg
    56281 atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt ggtcgccggt
    56341 taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat tgcggaaccg
    56401 gttatgtcta cgatgtgatt gccgattttg acggcaagcc ttccattacg ctgaccgaat
    56461 ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta ctgaaacagc
    56521 tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc
    56581 tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac attggtgaaa
    56641 gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag caagagcggt
    56701 tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac
    56761 ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt
    56821 gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctac
    56881 gcacgtagcc gtcaggcgtg gagactttga aacgaagtgg attaggtaat acagctgcca
    56941 gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg
    57001 cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga
    57061 tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc agcccttttc
    57121 gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga gaaatcgttg
    57181 aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc tcaatggaag
    57241 cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc accgccagtc
    57301 ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg taacgaaaat
    57361 tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag ggaacaggcg
    57421 caacgctaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca
    57481 aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac
    57541 cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag
    57601 catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg
    57661 acacggcaca tgctccgcgc ccatctcttc cagtacttct gcggtatcga tatcagtacc
    57721 gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat
    57781 aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc
    57841 aacggtgcat tcgctaccaa gactggcaaa attgcttaaa ttcttcgccg cgccaacccc
    57901 ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc
    57961 accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc
    58021 agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc
    58081 accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata
    58141 gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt
    58201 gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca
    58261 tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt
    58321 gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca
    58381 gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt
    58441 tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt
    58501 tatcttccca ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc
    58561 gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt
    58621 tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc atcttctcaa
    58681 acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca
    58741 tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct
    58801 catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga accgaaagcg
    58861 gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt
    58921 tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac
    58981 gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt
    59041 cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac
    59101 ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca
    59161 gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg
    59221 ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa
    59281 ccgtaatatc gccgcccata tttttcgcca gacgatgaga aacggccaga ccaataccgg
    59341 tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt
    59401 tatccagctc atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat
    59461 cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt
    59521 tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg
    59581 gtaatggcag cgtaggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg
    59641 agagattttc cagatcggca aggaagctgg tgaaatcaac cggctgatta tcaagctgga
    59701 ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga
    59761 cggccgaaac atggatagtt ttgagatatt tttcctgctc ggcggtgagt tcggtatcca
    59821 gcagaatgcg gctcaggccg acgataccgt tcagcggtgt acgcaattcg tgactgatgg
    59881 tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac
    59941 gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt
    60001 acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt
    60061 cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg acttttgcgg
    60121 cggcttccgg tgagtaaaca tcagcaggtt tcagatgaac cagttgtttt tcgctttttc
    60181 cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa
    60241 aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct
    60301 gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca
    60361 gttctgcttc cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt
    60421 ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc
    60481 gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg
    60541 cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct
    60601 gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca
    60661 gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact
    60721 gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat
    60781 acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac
    60841 ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt
    60901 cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca gcggagcaag
    60961 cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg
    61021 gcgaatcatt tctccggcag tgagcgtata atcctccagt tcaataccgt tcaaacgccc
    61081 cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat
    61141 gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc
    61201 gccaggcagg ccgacaatta agtgggaaca caccttcagc ccgcgctgac gtgccagctg
    61261 ggttgtacgc tgataacagg caaaatcatg gccgcggttg atgcgatgca gtgttttgtc
    61321 gtgggcggtt tgtagcccca gctccagcca cacttcgtaa ccctggtcct tatattcgca
    61381 aagcagatcc agcaccgcat ccggcacgca gtccgggcgg gtaccaacac acaaaccgac
    61441 aatattggcc tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa
    61501 ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc
    61561 ctggtgcgcc agttgctcgg caatggaacg atgctgctgc gcttcatcgg caaacgaggc
    61621 aacattacag aatgtgcagc caccacgccc gatggtaccg tcacggttag ggcagctaaa
    61681 accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc
    61741 aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc
    61801 caaagcctgc catttttagc tcaattcggc gatgacctag atcaatcgtc ccggcctgct
    61861 tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca cataaatcac
    61921 ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca aaaacagtag
    61981 catgaaacgt cattaccaat taaggcagta taaaatgctg gttttctcgt cagttcaagg
    62041 caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct ctaacgactc
    62101 tccttttagc cttaaagata aaatctattt taatttcagc catttaataa agaattttgc
    62161 gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc tttcccgata
    62221 agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat ttatgcagta
    62281 attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc
    62341 gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg
    62401 gttcggaagt ggggttcccg cagagcctgg gggaggttca cgatatgttg tacgataaat
    62461 cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc gaacctagcc
    62521 acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt ggcgcgattc
    62581 tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt
    62641 ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa aaactacgct gtcgggatgc
    62701 tcttcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt gaagaagaac
    62761 tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc
    62821 tgggtgaaat cgccctctcc tctctgccac gcattgagca aatttttgtg aacgccccgg
    62881 caggctggcg tccacgcgat atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa
    62941 agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg gtgaacatct
    63001 ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt gcggacctgc
    63061 gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc gtaccgcgct
    63121 ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac accatcaccg
    63181 gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctg atccctgacc
    63241 tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga ctccagttcc atggataaca
    63301 tgctggaact gctgctggca ggcgggatgg atatcatccg cgccatgcgc ctgttagtgc
    63361 cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc tttgacttta
    63421 actccatgca tatggagccg tgggatggtc cggcgggcat cgtgatgtcc gatggtcgct
    63481 tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc acgctacgtc attaccaaag
    63541 acaagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct gacgaagtgg
    63601 tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat cgacactcgc agtgggcgta
    63661 ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccacccttat aaagagtgga
    63721 tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta
    63781 gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac tacagcgcgg
    63841 aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga
    63901 tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt tacgactact
    63961 tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg
    64021 ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc
    64081 aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc aaacagctca
    64141 cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt gacgtcacta
    64201 aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa agccgaaaaa atggtacgta
    64261 gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc taaagatcgc ctgccggttc
    64321 cggctccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg
    64381 atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc gccgtgttgc
    64441 tgggtttcgg cgcgacggct atttatccgt accttgccta tgaaacgctg ggccgcctgg
    64501 tagacaccca cgcgattgcc aaagattatc gtaccgtgat gctcaactac cgtaacggca
    64561 tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc gcctcttacc
    64621 gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc ctgtgcttcc
    64681 agggcgcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag gatctgctga
    64741 atctgtcgaa acgtgcctgg ctggcgcgca agccgattag ccagggcggc ctgctgaaat
    64801 acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg
    64861 cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt aatgagcgtc
    64921 cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg
    64981 ctgatgttga accggcaagc gaactgttta aacgctttga taccgccgcg atgtctatcg
    65041 gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaatagc atcggcggta
    65101 actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc
    65161 gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg
    65221 acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt cagttgccgg
    65281 gcgacaaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga gtgacgctga
    65341 tctccccgcc gccgcaccac gatatctact ctatcgaaga cttagcgcag cttattttcg
    65401 acctcaagca ggttaacccg aaagcgatga tctccgttaa gctggtgtcc gaaccaggcg
    65461 ttggcaccat cgcgactggc gtggcgaaag cttatgcgga cttgatcacc atcgcgggct
    65521 atgacggcgg caccggcgca agcccgctgt cgtcagtgaa atatgcaggc tgcccgtggg
    65581 aactcggtct ggtggaaacc caacaggcgc tggttgccaa cggcttgcgt cataaaattc
    65641 gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat catcaaagcg gcgattctcg
    65701 gcgcagaaag cttcggcttc ggcactggcc caatggtggc gctcggctgt aaatatctgc
    65761 gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac tcaggatgac aaactgcgta
    65821 agaatcacta tcacggcctg ccgttcaagg tgactaatta ctttgagttt atcgcccgtg
    65881 aaacccgcga gctgatggca cagctgggag taacgcgtct ggtggatctg attggtcgca
    65941 ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg gcgctgtcga
    66001 agctgctgga gactgccgag ccacatgcag gtaagtcact ctactgcact gaaaacaacc
    66061 cgccgtttga taacggccta ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg
    66121 atgaacgcca gagcaaaacc ttctggttcg atatccgcaa caccgaccgt tctgtcggcg
    66181 cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca gccgatccta
    66241 tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtgtggaac gcgggcggcg
    66301 tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg gcgggcggct
    66361 taatcgccat tcatcctccg gttggttccg ccttccgcag ccatgaagca agcattatcg
    66421 gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg
    66481 aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg
    66541 gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc gttaacttcg
    66601 gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac
    66661 gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga tctggcgatc catgaagagc
    66721 atctgcgcgg tcttatcacc gagcatgttc agcataccgg ttctcagcgc ggtgaagaga
    66781 ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct ggttaaaccg aagtccagtg
    66841 atgtaaaagc actgctgggt caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc
    66901 agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg cagcgcgttg
    66961 atccgccaaa gaaaccgctg aagatccgta aaattgagtt tgttgaaatt tacgagccgt
    67021 tttccgaagg ccaggccaaa gcgcaggctg accgctgtct gtcgtgcggc aacccatact
    67081 gcgagtggaa atgcccggta cacaactaca tcccaaactg gctgaagctc gccaacgagg
    67141 ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa cacactgccg gaagtatgcg
    67201 gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg caccctgaac gatgagttcg
    67261 gtgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc gaaatgggct
    67321 ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt atcggtgcag
    67381 gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct
    67441 tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg
    67501 aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac
    67561 tcaataccga agtgggccgc gacgtacagc tggacgatct gctgagtgat tacgatgccg
    67621 tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg
    67681 atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta atgggctttg
    67741 gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggta gtacttggcg
    67801 gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg ccagggggca aagcacgtta
    67861 cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg
    67921 cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt gaagtgaacg
    67981 gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca
    68041 aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg gcagatgcgg
    68101 tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa cacagcgtcg
    68161 agctggattc acaaggccgc atcatcgctc cggaaggcaa cgacaacgct ttccagacca
    68221 gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg gtcgttaccg
    68281 ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa ctggctggaa gtttaagctc
    68341 tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt ctttctggaa
    68401 atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt gttaccggta
    68461 gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag ggacagataa
    68521 tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac ggcattattc
    68581 atctggtcga tagcgcccga acggaaacgg tacgtagcgt taacgcgctg atgaccgcga
    68641 cgtactggga aattggccga cggattgtcg aatttgaaca aggtggtgag gccagagcag
    68701 cttatggtgc acagttgatt gatcggctat cgaaggattt aagtcgacgg tataaacgtg
    68761 gggtctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt caacatgttg
    68821 aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag acaccatctg
    68881 cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga ggctgtttga
    68941 tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca tgcaaataaa
    69001 cgaagg
//
LOCUS       DADLXX010000005        65526 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLXX010000005 DADLXX010000000
VERSION     DADLXX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.005"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(127..606)
                     /gene="folK"
                     /locus_tag="JNX27_000319"
     CDS             complement(127..606)
                     /gene="folK"
                     /locus_tag="JNX27_000319"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7881657.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNX27_000320"
     CDS             complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNX27_000320"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7881658.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNX27_000321"
     CDS             complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNX27_000321"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7881659.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3023..3478)
                     /locus_tag="JNX27_000322"
     CDS             complement(3023..3478)
                     /locus_tag="JNX27_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7881660.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNX27_000323"
     CDS             complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNX27_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7881661.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNX27_000324"
     CDS             complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNX27_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7881662.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4979..7408
                     /gene="hrpB"
                     /locus_tag="JNX27_000325"
     CDS             4979..7408
                     /gene="hrpB"
                     /locus_tag="JNX27_000325"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7881663.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7604..10138
                     /gene="mrcB"
                     /locus_tag="JNX27_000326"
     CDS             7604..10138
                     /gene="mrcB"
                     /locus_tag="JNX27_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7881664.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10358..12601
                     /gene="fhuA"
                     /locus_tag="JNX27_000327"
     CDS             10358..12601
                     /gene="fhuA"
                     /locus_tag="JNX27_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7881665.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12652..13449
                     /gene="fhuC"
                     /locus_tag="JNX27_000328"
     CDS             12652..13449
                     /gene="fhuC"
                     /locus_tag="JNX27_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7881666.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13449..14339
                     /gene="fhuD"
                     /locus_tag="JNX27_000329"
     CDS             13449..14339
                     /gene="fhuD"
                     /locus_tag="JNX27_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7881667.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14336..16318
                     /gene="fhuB"
                     /locus_tag="JNX27_000330"
     CDS             14336..16318
                     /gene="fhuB"
                     /locus_tag="JNX27_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY7881668.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNX27_000331"
     CDS             complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNX27_000331"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7881669.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17858..19279
                     /gene="clcA"
                     /locus_tag="JNX27_000332"
     CDS             17858..19279
                     /gene="clcA"
                     /locus_tag="JNX27_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7881670.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19303..19368
                     /locus_tag="JNX27_000333"
     CDS             19303..19368
                     /locus_tag="JNX27_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881671.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19361..19705
                     /gene="erpA"
                     /locus_tag="JNX27_000334"
     CDS             19361..19705
                     /gene="erpA"
                     /locus_tag="JNX27_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7881672.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19752..20375)
                     /locus_tag="JNX27_000335"
     CDS             complement(19752..20375)
                     /locus_tag="JNX27_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7881673.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNX27_000336"
     CDS             complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNX27_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7881674.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNX27_000337"
     CDS             complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNX27_000337"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7881675.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21988..23505
                     /gene="dgt"
                     /locus_tag="JNX27_000338"
     CDS             21988..23505
                     /gene="dgt"
                     /locus_tag="JNX27_000338"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7881676.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23635..25059
                     /gene="degP"
                     /locus_tag="JNX27_000339"
     CDS             23635..25059
                     /gene="degP"
                     /locus_tag="JNX27_000339"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7881677.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25214..26370
                     /locus_tag="JNX27_000340"
                     /pseudo
     CDS             25214..26370
                     /locus_tag="JNX27_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26459..26845)
                     /locus_tag="JNX27_000341"
     CDS             complement(26459..26845)
                     /locus_tag="JNX27_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7881678.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNX27_000342"
     CDS             complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNX27_000342"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7881679.1"
                     /translation="MQQLQNIIETAFERRADITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAIIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNX27_000343"
     CDS             complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNX27_000343"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7881680.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNX27_000344"
     CDS             complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNX27_000344"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7881681.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31910..32635
                     /gene="rpsB"
                     /locus_tag="JNX27_000345"
     CDS             31910..32635
                     /gene="rpsB"
                     /locus_tag="JNX27_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7881682.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32893..33744
                     /gene="tsf"
                     /locus_tag="JNX27_000346"
     CDS             32893..33744
                     /gene="tsf"
                     /locus_tag="JNX27_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7881683.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33891..34616
                     /gene="pyrH"
                     /locus_tag="JNX27_000347"
     CDS             33891..34616
                     /gene="pyrH"
                     /locus_tag="JNX27_000347"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7881684.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34908..35465
                     /gene="frr"
                     /locus_tag="JNX27_000348"
     CDS             34908..35465
                     /gene="frr"
                     /locus_tag="JNX27_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7881685.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35557..36753
                     /gene="ispC"
                     /locus_tag="JNX27_000349"
     CDS             35557..36753
                     /gene="ispC"
                     /locus_tag="JNX27_000349"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7881686.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36942..37700
                     /gene="ispU"
                     /locus_tag="JNX27_000350"
                     /gene_synonym="uppS"
     CDS             36942..37700
                     /gene="ispU"
                     /locus_tag="JNX27_000350"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7881687.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37713..38570
                     /gene="cdsA"
                     /locus_tag="JNX27_000351"
     CDS             37713..38570
                     /gene="cdsA"
                     /locus_tag="JNX27_000351"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7881688.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38582..39934
                     /gene="rseP"
                     /locus_tag="JNX27_000352"
     CDS             38582..39934
                     /gene="rseP"
                     /locus_tag="JNX27_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7881689.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39964..42396
                     /gene="bamA"
                     /locus_tag="JNX27_000353"
     CDS             39964..42396
                     /gene="bamA"
                     /locus_tag="JNX27_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7881690.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42518..43003
                     /gene="skp"
                     /locus_tag="JNX27_000354"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42518..43003
                     /gene="skp"
                     /locus_tag="JNX27_000354"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7881691.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43007..44032
                     /gene="lpxD"
                     /locus_tag="JNX27_000355"
     CDS             43007..44032
                     /gene="lpxD"
                     /locus_tag="JNX27_000355"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7881692.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44137..44592
                     /gene="fabZ"
                     /locus_tag="JNX27_000356"
     CDS             44137..44592
                     /gene="fabZ"
                     /locus_tag="JNX27_000356"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7881693.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44596..45384
                     /gene="lpxA"
                     /locus_tag="JNX27_000357"
     CDS             44596..45384
                     /gene="lpxA"
                     /locus_tag="JNX27_000357"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7881694.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45384..46532
                     /gene="lpxB"
                     /locus_tag="JNX27_000358"
     CDS             45384..46532
                     /gene="lpxB"
                     /locus_tag="JNX27_000358"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7881695.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46529..47125
                     /gene="rnhB"
                     /locus_tag="JNX27_000359"
     CDS             46529..47125
                     /gene="rnhB"
                     /locus_tag="JNX27_000359"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7881696.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47162..50644
                     /gene="dnaE"
                     /locus_tag="JNX27_000360"
     CDS             47162..50644
                     /gene="dnaE"
                     /locus_tag="JNX27_000360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7881697.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50657..51616
                     /gene="accA"
                     /locus_tag="JNX27_000361"
     CDS             50657..51616
                     /gene="accA"
                     /locus_tag="JNX27_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7881698.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51715..53856
                     /gene="ldcC"
                     /locus_tag="JNX27_000362"
     CDS             51715..53856
                     /gene="ldcC"
                     /locus_tag="JNX27_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY7881699.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53913..54302
                     /locus_tag="JNX27_000363"
     CDS             53913..54302
                     /locus_tag="JNX27_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7881700.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54367..55665
                     /gene="tilS"
                     /locus_tag="JNX27_000364"
     CDS             54367..55665
                     /gene="tilS"
                     /locus_tag="JNX27_000364"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7881701.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNX27_000365"
     CDS             complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNX27_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7881702.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55961..56161)
                     /locus_tag="JNX27_000366"
     CDS             complement(55961..56161)
                     /locus_tag="JNX27_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7881703.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56327..56872
                     /locus_tag="JNX27_000367"
     CDS             56327..56872
                     /locus_tag="JNX27_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7881704.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56869..57291
                     /gene="arfB"
                     /locus_tag="JNX27_000368"
     CDS             56869..57291
                     /gene="arfB"
                     /locus_tag="JNX27_000368"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7881705.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57305..58015
                     /gene="nlpE"
                     /locus_tag="JNX27_000369"
                     /gene_synonym="cutF"
     CDS             57305..58015
                     /gene="nlpE"
                     /locus_tag="JNX27_000369"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7881706.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58170..58994)
                     /locus_tag="JNX27_000370"
     CDS             complement(58170..58994)
                     /locus_tag="JNX27_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY7881707.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNX27_000371"
     CDS             complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNX27_000371"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7881708.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNX27_000372"
     CDS             complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNX27_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7881709.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNX27_000373"
     CDS             complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNX27_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7881710.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNX27_000374"
     CDS             complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNX27_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7881711.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNX27_000375"
     CDS             complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNX27_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7881712.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNX27_000376"
     CDS             complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNX27_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7881713.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64822..65397
                     /gene="gmhB"
                     /locus_tag="JNX27_000377"
     CDS             64822..65397
                     /gene="gmhB"
                     /locus_tag="JNX27_000377"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7881714.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15479 a        16235 c        18059 g        15753 t
ORIGIN      
        1 gggattgata tttatctgcg ggaatttata ttatgtttct gcaaaaatgt ttaaactaaa
       61 atttttatta aggaaagaga ggatgtatga ttttcagaaa atatttatat tcgtaatata
      121 aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt
      181 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
      241 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      301 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      361 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgggcgaagt
      421 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      481 gcggtaaaac gaagaaacgg caagaatgcg gctttcaggg atatcgccta atgctttcag
      541 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      601 tgtcatgtgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acgacgacgc
      661 ggtgacggtt cttcatccag ctcgttaagc atcccttttt gatctggtgg tgcggaaacc
      721 tggaactcac cccaccattt caccagacgc tgcagttcag cattacgttc aacttcagct
      781 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      841 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      901 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      961 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1021 agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta
     1081 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1141 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1201 cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1261 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1321 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1381 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1441 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1501 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1561 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1621 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1681 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1741 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1801 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1861 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac tgtcacctgt
     1921 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1981 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2041 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2101 actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2161 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2221 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2281 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2341 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2401 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2461 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     2521 gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2581 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2641 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2701 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2761 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2821 cttctatgcg taccagccag cgaccttgcc gggcgcgacc ctgcaaatag ctgccgagcg
     2881 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2941 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3001 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3061 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3121 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3181 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3241 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3301 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3361 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3421 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3481 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3541 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3601 gaaaagcgta ttcgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3661 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3721 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3781 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3841 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3901 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3961 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttcggagc
     4021 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4081 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc
     4141 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4201 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4261 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4321 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     4381 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4441 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg ggaatgtcac
     4501 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4561 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4621 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4681 cgtgagtgtg aaacctggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4741 ctctgcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4801 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcgggtttc
     4861 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4921 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4981 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     5041 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5101 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5161 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5221 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5281 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     5341 ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc tgttactcga
     5401 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     5461 tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag ggcgctcgtt
     5521 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgttgc
     5581 ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat ttttacctgg
     5641 cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca gtgatgtggt
     5701 gctctgcccg ctgtatggcg cgttgtcact gaacgatcag cgaaaagcga tcctcccggc
     5761 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5821 cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgct ttgatccgcg
     5881 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     5941 cgggcgcgcc gggcgtctgg agccgggtat cagcctgcat ttaatcgcca aagaacaagc
     6001 agaacgcgcc gcggcgcaaa gtgagccgga gatcttacaa agcgatcttt ccggtttgct
     6061 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6121 gccaacggtg aatctactgg ccgcgaaacg cctgttacaa atgctggggg cgctggaggg
     6181 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6241 ggcaatgctg gttaacgcaa agagcgatga cgaagctgct accgcggcaa aaattgccgc
     6301 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6361 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgcggcgg
     6421 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6481 tcatcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6541 tgccgacgac gcgctaagcc gccacgaatg gttgatcgca cagttattat tgcagggcag
     6601 cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg aattagtaca
     6661 acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg cgcaaggtac
     6721 gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc agccgctggc
     6781 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt
     6841 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6901 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttgt tggcgacgct
     6961 ggaaacgtgg ctgctgccac atatgaccgg cgtacattca ctacgcggcc tgaaatcact
     7021 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7081 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7141 agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccactaatcc
     7201 gacgatcgcc caggggcgcg tgccgctggt gctgaagttg ctttcccctg cccaaaggcc
     7261 gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg aggtgcaaaa
     7321 ggagatgaaa gggcgttatc ccaaacacgt ctggccgaac gacccggcaa acacggcacc
     7381 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg
     7441 gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga
     7501 gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa
     7561 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7621 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7681 tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat
     7741 gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7801 atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta
     7861 tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc ccgcggcagt
     7921 ttatggccga atggtcaacc ttgaaccaga tatgaccatc agcaagaacg aaatggtgaa
     7981 gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac
     8041 cgtgcaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga
     8101 agggcaggtg cgtgcgcgtc tgacctttga tggtgatcat ctggcgacca tcgtcaatat
     8161 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc
     8221 ctcgccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt
     8281 ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa tcagtctcta
     8341 ctccattggg cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc agggtgcgag
     8401 tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8461 taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8521 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg
     8581 cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca
     8641 gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8701 gaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat
     8761 tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgtggtgg
     8821 ggtgatctct cctcagccag cctttatgca gttggtgcgt caggagttgc aggcaaaact
     8881 gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8941 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     9001 gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc
     9061 gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     9121 ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat
     9181 ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9241 ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt
     9301 ggatgcgttg acacgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct
     9361 gcctgcggtt acggagacct ggatcaaact gggcgtaccg aaagatcagt tgcatccggt
     9421 tccggcaatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca
     9481 gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga
     9541 agatggtaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc
     9601 ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct
     9661 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9721 agatacctgg tttgcaggca ttgacggcag cacggtgacc atcacctggg taggccgtga
     9781 taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta
     9841 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9901 gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9961 gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa
    10021 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
    10081 gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca
    10141 tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc ctatctaaga
    10201 tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt
    10261 accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg
    10321 tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca
    10381 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc
    10441 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10501 tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcggc agtcagctac
    10561 cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc
    10621 cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg
    10681 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac catctgatca ttcgcggttt
    10741 tgcggcagaa ggccaaagcc aaaataacta tctgaatggc ctgaagttgc agggcaactt
    10801 ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc
    10861 ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg
    10921 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10981 gactggtttt gactttagcg atgcgttgga tgatgacggt gtttactctt atcgcctgac
    11041 cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    11101 tgcaccggcg ttcacctggc gtccggatga taaaaccaat ttcaccttcc tttcttactt
    11161 ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    11221 gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta
    11281 ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac
    11341 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11401 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11461 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11521 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct
    11581 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga
    11641 ctctgtgcca ctgctcaatc tgtacaatcc ggtgaatccc gatttcgact tcaatgccaa
    11701 agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta
    11761 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctaggcggtc gttatgactg
    11821 ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca
    11881 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag
    11941 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    12001 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    12061 agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg
    12121 ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa
    12181 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata
    12241 caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12301 gctgtgggct gactatacct tctttgacgg tccgctttca ggtttgacgc tgggcaccgg
    12361 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12421 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12481 cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac
    12541 ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct ttcgtttcta
    12601 atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa
    12661 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12721 cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12781 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca tcagcctccg
    12841 tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt
    12901 gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12961 gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca
    13021 gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    13081 ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg
    13141 caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag
    13201 gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc
    13261 gtgttgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcagt
    13321 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13381 atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt
    13441 gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc
    13501 tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta
    13561 ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg
    13621 gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag
    13681 tgattgacgt cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt
    13741 cgtttatggt ctggtcggca ggatatggcc cttcatcaga aatgctggca cgtattgcgc
    13801 cgggtcgcgg atttaacttc agtgacggca aacatccgtt ggcgatggcg cgtaaatcgc
    13861 tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat
    13921 acgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac
    13981 tgctgacgac gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc
    14041 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    14101 gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa agacgttgat gtgctctgtt
    14161 ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca
    14221 tgccgtttgt ccgtacaggg cgctttcagc gcgtacccgc agtctggttt tatggtgcaa
    14281 cgctttcggc aatgcacttt gtgcgtgttc tggataacgc catcggaggt aaagcgtgag
    14341 taaacgaatt gcgcttttcc cggcgttatt gctggcgctg ttagtgattg tcgctacggc
    14401 gctcacctgg atgaacttct cacaggcgct gccgcgcagt cagtgggcgc aggccgcctg
    14461 gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct
    14521 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14581 actgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg cgcaactggg
    14641 gattaccgtc accacgctct gggcgatccc tagcgcgatg gcgagccagt ttgctgcgct
    14701 ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga aacggctgtc
    14761 gccggtaacg ctgattctcg cggggttggt agtgagcctt tattgcggcg caatcaatca
    14821 gttactggtt atcttccatc atgaccagtt gcaaagcatg tttctgtgga gcactggaac
    14881 gctgacgcaa accgactggg gcggtgttga gcgtttatgg ccgcagttgc tgggcggtgt
    14941 gatgctgacg ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc
    15001 gcgcaatctc gggctggcct tgtcgcttgc gcgtctggcg gcgctgtcgc tggcgattgt
    15061 catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc
    15121 gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgtt
    15181 gattggtgcg ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg
    15241 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct
    15301 gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gttaacgatc gtgtcgcgac
    15361 tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgttgt tgatggctgt
    15421 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15481 gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt
    15541 catgctggcg gtagcgggct gtattattca gcgactgacc ggcaacccga tggcaagccc
    15601 ggaagtgctg gggattagct ccggcgcggc gtttggtgtg gtgttaatgc tgtttctggt
    15661 accgggtaat gcctttggct ggttgttacc cgcaggcagt ctcggggcgg cggtgatgct
    15721 gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca tgttactggc
    15781 ggggatggcg ttaagtaccg cgttcaccat gcttttgata atgttgcagg caagtggtga
    15841 cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15901 tcaggtctgg cgcaccggaa ttgtgatggt gattttgctg gcgattactc cgctgtgccg
    15961 ccgctggctg accattttac cgctgggtgg tgataccgcg cgggcggtag ggatggcgct
    16021 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat
    16081 gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    16141 ctttcgacgg acgatgccac atatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    16201 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg cggggctgct
    16261 gtcgaccttt atcggtgcgc catattttat ctatttgctg agaaagcaga gccgttaata
    16321 gaatgcctcg cccggtgggc gaggcaaaat tatcacaact tcgcgaacac ccgacgtgca
    16381 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa gcccgcttca
    16441 aacgctgacg gtgccaggta aacaccttca tccagcatca tatggaagaa acgcttaaaa
    16501 cgctcgacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16561 aagataccga acataccacc gacgtggtta acgaccagcg gaattcccgc ttcttctgcc
    16621 gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaatgccc
    16681 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg gttaccggaa
    16741 agcgtacccg cctgatagac cggacctgtc ggggccagcg catccattac atcacgacga
    16801 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt ttccgaggca ggttaaatct
    16861 ggcactacgc cgaaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16921 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc tggcaggaac
    16981 tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    17041 atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt ataagtacag
    17101 gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg
    17161 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    17221 aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17281 gcctcggtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17341 agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg
    17401 gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag
    17461 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17521 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17581 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17641 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17701 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17761 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17821 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17881 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17941 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttatcgggct
    18001 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct
    18061 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    18121 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag ggggatcggg
    18181 gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt
    18241 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga
    18301 aggaccaacc gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct
    18361 gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc
    18421 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18481 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18541 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc
    18601 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18661 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18721 taccaaatgg gtactgatgg ggggggcgat tggcggtctg tgtggattgc tggggtttgt
    18781 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18841 cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt
    18901 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18961 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac
    19021 gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg
    19081 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    19141 cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat actcggcgat
    19201 tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19261 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19321 ttagaatttg cccgattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19381 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19441 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19501 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19561 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactacac
    19621 cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19681 ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19741 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19801 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19861 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19921 gttgcgtaga tttctgtacg taaaatcatg gagatttcgc gggccagtac atcacgaatg
    19981 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    20041 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    20101 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    20161 ctggtgacca tggcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20221 tccagcgcca tgtcgcgaat tgttccgtcg cctactgcgg taaccacgcc cagtaccaga
    20281 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20341 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20401 aaaggtcagg cactaatcta cctgtgaaag cgcattacag agctgttgtg ctgcgaggat
    20461 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20521 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20581 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20641 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20701 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20761 agcatattgc gctttcaatt gcacgtactg atccagcagg gattgcgcgg cttgttcggc
    20821 cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20881 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20941 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    21001 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21061 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21121 gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcga gaaaagacag
    21181 ggcgaccagc gccctgaaca gtgatttagc catgtgccag tttctgcacc agtgactcaa
    21241 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21301 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21361 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21421 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21481 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21541 cagctttaaa gcctgtcgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21601 catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21661 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21721 gcagagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21781 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt
    21841 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21901 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21961 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    22021 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22081 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22141 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22201 gaagtccagc aggtgggacg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22261 aaattgctgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag
    22321 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg
    22381 ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22441 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22501 ctgcggcgta agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22561 ctggtgcata cattgatgcg gatgaatctc acttgggcac aggttggcgg tattttaaaa
    22621 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22681 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22741 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc
    22801 tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22861 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22921 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22981 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    23041 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    23101 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtattt
    23161 agccatccgg atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23221 gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa
    23281 gaacgggtga aacgtttccc tattgagtcg cgcttattcc acaaactctc gacgcgccat
    23341 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23401 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23461 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23521 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg ggctataaaa
    23581 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23641 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23701 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23761 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23821 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23881 tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat
    23941 ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    24001 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24061 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    24121 gcgctgatcc aaatccagaa cccgaaaaaa ctgaccgcaa ttaagatggc ggattctgat
    24181 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggcgagacg
    24241 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga aaactacgaa
    24301 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24361 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24421 ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24481 tacgggcagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg
    24541 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24601 tcctcggctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24661 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24721 ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga actgcagcag
    24781 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg
    24841 agcaacaaag gtaaagatca gggcgtggta gtgaacaacg tgaagacggg cactccggct
    24901 gcgcagatcg gcctgaagaa aggtgatgtg attattggtg cgaaccagca ggcagtgaaa
    24961 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    25021 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    25081 atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat acatcttcat
    25141 catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25201 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25261 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25321 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25381 acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg gtgtgcggca
    25441 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25501 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25561 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aagaactggt
    25621 gatgaacctg attcaggcag aggagaatac tcccgcactt ctgaatgggc gcaacggctg
    25681 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    25741 ggcgtggaca gcgcaatggc ggagttacaa caactgcaaa acgcgctgac tacgcccgag
    25801 cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    25861 aactcttttg ggcgctggga tgcagaagat catcgtaagc gagttgaaca actgattacc
    25921 cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac tgggcaacta ttttaccggt
    25981 cctagcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag
    26041 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    26101 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    26161 atggacaata acggcttgct gcgacgaacg ctggcggcgt ggtttcgcca caatgtgcaa
    26221 ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ccctggagta tcggcttaat
    26281 cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat
    26341 gtggcgttac aactggatga agagcggtag attatgcgtt aaggttgagg cggtgctggc
    26401 tcattcccct cagcccgaag gggagagggg aatgcccgag ttaaccgtca attcgggatt
    26461 atttacgcgt tagtttttcc agatcggctt caatctcgct gatcttattg gttacgactg
    26521 actccaggtg acgtaagtcg tcgaggatct tacgcttaag atcaacttcg ctgcggtcac
    26581 gctgacagat ttgatcaagc tcatcaatga tataccgtag attcgggctg atttcctgga
    26641 cttctttata accctgaccc acaccatcag cgacgaccgt tttacgctga cgcggatatt
    26701 taaacttaac gctcttggcg aaaaactcgc ctttgtcttt ctggaaatag attttcagaa
    26761 tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat ttcttcagga ttggtaatac
    26821 ccagactttt cagattgtcg tacatagcgt tacctcaaaa tgagtcagta aattgtgctt
    26881 attttagcat ttggcttggc ccgcccggct cgactgtgat cgcagaaagc ctttttcttc
    26941 cagacttcta tcatggcgta aaaatcaaaa aattacctgc tttattgtgg cgatattcca
    27001 tgggatggat tagcacattt atcttcgttt tgcgaattac atgacagtcc gaaacaagat
    27061 gaaaaagtat ttaaaaaagg gggaggaaga atgagtagga cgaattattc tgagccagaa
    27121 agcatacggg cagcatcatg ctgcccgtat atgtactctt aatcgatggt acgcagcagt
    27181 tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aatcgcacag
    27241 tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc
    27301 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca
    27361 atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    27421 gagcgtgcgc caatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc
    27481 agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa
    27541 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgtta
    27601 acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct
    27661 ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc
    27721 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    27781 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    27841 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    27901 cgggtaacgg tgtctgcatt ggctggcgtg atatcggcac ggcgttcaaa agcggtttca
    27961 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    28021 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    28081 ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    28141 gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc
    28201 gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg
    28261 gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga
    28321 cggggctgcg gtggctgcca gctactttgc gtcagcacct gctccagacc aaaccgaata
    28381 acctcatggc gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc
    28441 atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    28501 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    28561 tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc
    28621 tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    28681 gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    28741 agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    28801 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    28861 cagctattcc acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    28921 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc
    28981 tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca
    29041 accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga
    29101 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    29161 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    29221 tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    29281 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    29341 ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    29401 ccggggtgac gcagaatgct caaaaacagt tttcgtgctt gcggaatatt acacagcggt
    29461 tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aataccggtg
    29521 atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata
    29581 aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt
    29641 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    29701 atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg
    29761 accggttcgt tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg
    29821 aataacagac gattatcgta acggctgacg accagatgca gggcaaagcg aatatgccac
    29881 agtatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaacccg
    29941 accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtga
    30001 atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta gctggtgcca
    30061 tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa
    30121 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca
    30181 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccaac
    30241 atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc
    30301 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    30361 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcaacc
    30421 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg
    30481 tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca
    30541 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac ggttaattca
    30601 tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggagag agcggtgttt
    30661 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta
    30721 aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt
    30781 gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa tagtatgctc
    30841 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    30901 gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag
    30961 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg
    31021 accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    31081 ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc gtagcgccag
    31141 gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg gcttaccgac
    31201 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt
    31261 aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc acaccacttc
    31321 attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc
    31381 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    31441 cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    31501 cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg
    31561 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    31621 agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcga
    31681 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa
    31741 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    31801 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    31861 atgggatacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    31921 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    31981 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    32041 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg tttctcgcaa
    32101 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    32161 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    32221 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    32281 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    32341 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    32401 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacttgggta ttccggtatt
    32461 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    32521 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    32581 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    32641 cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg
    32701 ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga
    32761 aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca
    32821 tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc
    32881 gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact
    32941 ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg
    33001 gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt
    33061 gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt
    33121 aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt
    33181 ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa
    33241 gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct
    33301 gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt
    33361 gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc
    33421 aagccagaat tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag
    33481 gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt
    33541 gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg
    33601 gaaccaagca aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc
    33661 atccgtttcg aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt
    33721 gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt
    33781 tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa
    33841 tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca
    33901 atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
    33961 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac
    34021 tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg
    34081 ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga
    34141 ccgtaatgaa cggcttggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc
    34201 tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca
    34261 gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta
    34321 ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
    34381 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt
    34441 acgagcaatt gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg
    34501 ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg
    34561 gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc
    34621 cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta
    34681 taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca
    34741 aaagtacgtg aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat
    34801 aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt
    34861 gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata
    34921 tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca
    34981 gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt
    35041 acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc
    35101 tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcct
    35161 ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc
    35221 tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc
    35281 gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag
    35341 ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg
    35401 atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt
    35461 tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg
    35521 ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc
    35581 tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc
    35641 cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa
    35701 ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg
    35761 ctacagcaac aggggagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg
    35821 gcagcgcttg aggaggttga tcaggtgatg gcagccattg ttggtgctgc tgggctgtta
    35881 cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg
    35941 gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg
    36001 gtcgatagcg aacataacgc tatttttcag agtttaccgc aacctatcca gcataatctg
    36061 ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc
    36121 cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca agcttgccgt
    36181 catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa
    36241 ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg
    36301 ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg
    36361 gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat
    36421 cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc
    36481 gcaccggatt atgagcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc
    36541 caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg
    36601 caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat
    36661 atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gagtgaagtc
    36721 gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc
    36781 tatttgttag cgtagggctt caatgatata gtctgcgcca tctgatcgta agtagttggc
    36841 tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt
    36901 cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac
    36961 cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca
    37021 atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa
    37081 aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaagcg ttaacgctgt
    37141 atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac
    37201 tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc
    37261 gtattattgg cgataccagt cgttttaact cgcgtttgca agaacgtatt cgtaaatctg
    37321 aagcgctaac agccggaaat accggtctga cgctgaatat tgcggcgaac tacggtggac
    37381 gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc
    37441 aaccagatca gatagatgaa gagatgttaa accagcatgt ctgtatgcat gaactggccc
    37501 ctgtagattt agtaattagg actgggggag agcatcgcat tagtaacttt ttgctttggc
    37561 aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag
    37621 actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc
    37681 ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt
    37741 ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca
    37801 ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta
    37861 ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc
    37921 tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt
    37981 gggcttcgtt gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg
    38041 cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc
    38101 ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg
    38161 gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata
    38221 tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct
    38281 ggcaaggctt tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt
    38341 gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag
    38401 cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca
    38461 gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg
    38521 cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa
    38581 tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac
    38641 cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt
    38701 ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt
    38761 tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt
    38821 cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac gagtggcgat
    38881 tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt
    38941 tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca attcgatagc
    39001 tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc
    39061 tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat
    39121 tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg
    39181 ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc
    39241 gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt
    39301 gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt
    39361 tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg
    39421 gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg
    39481 ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca
    39541 gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct
    39601 gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg
    39661 gccgatttct atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct
    39721 gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt
    39781 acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc
    39841 cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg
    39901 gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata
    39961 acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac
    40021 ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt
    40081 ggtgcggccc tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc
    40141 agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt
    40201 gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc
    40261 tccggtaaca aatcggtgaa agatgacatg ctaaagcaaa acctcgaggc ttctggtgtg
    40321 cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac
    40381 ttctactaca gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg
    40441 cgcaaccgtg ttgacctaaa actggtgttc cgggaaggtg tgtcagctga aatccagcaa
    40501 attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg
    40561 cgtgacgaag tgccgtggtg gaatgtggta ggcgatcgta aataccagaa acagaaactg
    40621 gcgggcgacc ttgaaaccct gcgcagctac tatctggacc gcggttatgc ccgtttcaac
    40681 atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac
    40741 atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg
    40801 cactccgctg aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcact
    40861 aaagtgacca aaatggaaga tgacattaaa aagcttctcg gtcgctatgg ttatgcctat
    40921 ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg
    40981 aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc
    41041 tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gttggggagc
    41101 gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat
    41161 actgataccc aacgtgttcc gggtagcccg gaccaggttg atgtagtcta caaggtgaaa
    41221 gaacgtaaca ctggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc
    41281 ttccaggctg gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac
    41341 gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc
    41401 gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc
    41461 gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatt
    41521 aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg
    41581 cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacttct
    41641 gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac
    41701 aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg
    41761 accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg
    41821 ccgatcgatg acgatcacaa atgggttgtt ctgggtcgta cccgctgggg ttatggtgat
    41881 ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc
    41941 gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc
    42001 agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg
    42061 tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc
    42121 accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc attcttctgg
    42181 gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac
    42241 tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca
    42301 ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca
    42361 gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt
    42421 ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg
    42481 caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc
    42541 aggtcttggt ttggcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa
    42601 tatgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa
    42661 tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat
    42721 gaaaaaactg cagtccatga aagcgggcag cgatcgcacc aaactggaaa aagacgtgat
    42781 ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg
    42841 ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc
    42901 caacagccag gatatcgatc tggttgttga tgcaaacgct gttgcttaca acagcagcga
    42961 tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg
    43021 actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat
    43081 caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc
    43141 aaaataccgt gagcatttag gcttgtgcca agcgtccgcg gttgtcatga cccaggacga
    43201 tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg
    43261 catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt
    43321 gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga
    43381 gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa
    43441 cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca
    43501 gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta
    43561 tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga
    43621 tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactgttat
    43681 tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga
    43741 caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg
    43801 catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt
    43861 tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat
    43921 tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga
    43981 catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat
    44041 cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat
    44101 tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg
    44161 cagattgaag agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc
    44221 gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag
    44281 ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa
    44341 gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt
    44401 gagctgtact acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc
    44461 gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa
    44521 ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc
    44581 cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa
    44641 gagggcgcgt caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat
    44701 gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa
    44761 attggccgcg ataatgagat ttatcagttt gcctccatcg gcgaagttaa ccaggatttg
    44821 aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc
    44881 gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac
    44941 ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc
    45001 gccaacaacg caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc
    45061 atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc
    45121 ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc
    45181 ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc
    45241 cgcaatgcgt ataagctaat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa
    45301 attgctgaac tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc
    45361 tcaacgcgcg gtctgattcg ttaatgactg accagcgtcc attaacgatt gccctggtcg
    45421 ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg
    45481 tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag
    45541 cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc
    45601 gtcgcttact gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg
    45661 tttttgttgg tattgatgcg cccgacttca atattactct tgaaggtaac ctcaaaaagc
    45721 agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg
    45781 ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt
    45841 tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct gatgccatgc
    45901 cgttagatcc agataaaaat ggtgcccgtg atgtgctggg gatcccttac gatgcccact
    45961 gtctggcatt gttgccgggc agccgtggtg cagaagtcga aatgcttagt gccgatttct
    46021 tgaaaactgc ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccgctgg
    46081 tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagacctgt
    46141 cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagcgat gcggcactac
    46201 tggcgtcggg gacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat
    46261 atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct
    46321 cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg
    46381 agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg
    46441 cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg
    46501 cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct
    46561 ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc
    46621 ggtgatcctt gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga
    46681 aaaacgccgt ctggcgctct atgaagagat caaagagaaa gcgttgagct ggagtctggg
    46741 ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat
    46801 gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg
    46861 ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggggatagcc gcgtaccaga
    46921 aatcagtgcc gcgtctatcc tagcgaaagt gacgcgtgac gccgaaatgg cggcgctgga
    46981 tattgttttc ccgcaatatg gttttgccca acataaaggg tatccaaccg cttttcatct
    47041 ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg ggcctgtcaa
    47101 acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa
    47161 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    47221 cttggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    47281 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    47341 agggattaag cctatcgtcg gggcagattt taatgtccag tgcgacctgc tgggtgatga
    47401 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tggcgttgct
    47461 gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg
    47521 gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    47581 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    47641 acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag
    47701 ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa
    47761 cgatgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    47821 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    47881 gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc ccagaagccc ttgccaacac
    47941 cgttgagatc gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca
    48001 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct
    48061 ggaagagcgt ctggcatttt tattccctga cgaggaagaa cgtcttaaac gccgcccgga
    48121 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggtt tcccgggcta
    48181 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtaggacc
    48241 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    48301 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    48361 tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgaac acgtggcgga
    48421 catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc
    48481 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg accgtatctc
    48541 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    48601 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    48661 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac
    48721 caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca
    48781 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    48841 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    48901 cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    48961 gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    49021 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    49081 tggtccgttg cagtcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga
    49141 gatctcctat ccggacgtgc agtggcagca tgaaagcctg aaatcggtac tggagccaac
    49201 ctacggcatt atcttgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    49261 taccctcggt ggcgcggata tgctgcgccg tgcgatgggt aagaaaaagc cggaagagat
    49321 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    49381 ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca
    49441 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc
    49501 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    49561 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    49621 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg
    49681 ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg
    49741 cgaactgttt gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga
    49801 aaaactgatc atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa
    49861 ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    49921 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    49981 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    50041 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    50101 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacagctg cggggctcgt
    50161 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    50221 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    50281 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    50341 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    50401 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    50461 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    50521 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    50581 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    50641 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    50701 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    50761 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt
    50821 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccgcagc gtccttatac
    50881 cctagattac gttcgcctgg catttgatga atttgacgaa ctggctggcg accgtgcgta
    50941 tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat
    51001 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    51061 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    51121 gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    51181 tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgccttg gcgtaccggt
    51241 agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga
    51301 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    51361 cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    51421 tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc
    51481 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    51541 cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    51601 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    51661 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    51721 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    51781 gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg
    51841 ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac
    51901 agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    51961 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    52021 tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac
    52081 gaatatcttg ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag
    52141 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    52201 gttggctgtc tgttttatga ttttttcggc gggaataccc ttaaggctga tgtctctatt
    52261 tcggtcaccg agcttggttc gttgcttgac cacaccgggc cacacctgga agcggaagag
    52321 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    52381 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    52441 aactgccata aatcactggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    52501 aaaccgacgc gtaatgcgtt ggggattctg ggggggatcc cgcgccgtga atttactcgc
    52561 gacagcatcg aagagaaagt cgctgccact acgcaagcac aatggccggt tcatgcggtg
    52621 atcaccaact ccacctatga tggtttgctc tacaacaccg actggatcaa acagacgctg
    52681 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt atactcattt tcatccaatc
    52741 taccagggta aaagtggtat gagcggcgag cgtgttgcgg gaaaagtttt cttcgaaacg
    52801 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cactgatcca cattaaaggc
    52861 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    52921 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    52981 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggctg
    53041 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    53101 gaatgctggc ccgttgcgcc tggcgaacaa tggcacggct ttaacgatgc ggatgccgat
    53161 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    53221 aatatgagtg aggaggggat cccggcggcg ctggtagcaa aattcctcga cgaacgtggg
    53281 atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat tggcatcgat
    53341 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc ttacgatctc
    53401 aacctgcgga tcaaaaatat gctgcccgat ctctatgctg aagatcccga tttctaccgc
    53461 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    53521 tccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    53581 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    53641 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga
    53701 gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta
    53761 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    53821 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    53881 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    53941 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    54001 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    54061 ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc
    54121 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc gatgcggcag
    54181 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    54241 aaaaacgctt caccttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    54301 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    54361 accatcatga cactcacgct caatagacca cttctcacct cacgccagat tctggtggcc
    54421 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    54481 aatccgggcg tcactctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat
    54541 gcctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa
    54601 cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc aggcgcggca ggcgcgttat
    54661 caggcatttg cccgtaccct attgcccggc gaagtgctgg tcaccgcgca acatctcgac
    54721 gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    54781 gctatggcgg aagtttcgga gtttgctgga acacggctta tccgcccgtt gcttgcccgc
    54841 acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    54901 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    54961 cagcagcgtt ggccgcattt tgcggaagca acggcccgca gcgccacact ttgtgctgaa
    55021 caagagagtc tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag
    55081 gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatt
    55141 cgccgctggc tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcgcatc
    55201 tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt gggcgccttt
    55261 gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa
    55321 accattgtgc tgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaacc
    55381 gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    55441 cgtttcaaag cgccaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag
    55501 aaaatctggc aagagttagg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    55561 tatggagaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    55621 ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    55681 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc
    55741 gatttcaggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    55801 ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    55861 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    55921 aagattatcg taatcatcac aattaattgg ttgatacgta tcattcattg acataatcgc
    55981 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    56041 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    56101 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    56161 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    56221 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    56281 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    56341 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc
    56401 ttctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    56461 ggcgtggctg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    56521 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    56581 gctgccggat gagcgacgaa ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt
    56641 tacctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca
    56701 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    56761 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct ggttatcgga
    56821 tgataagaat aatctggaag ttaacttaac cgcctggcaa caaccttcat gattgtgatt
    56881 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    56941 gctggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    57001 gcgcccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    57061 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    57121 gaagccgctc tggcccggct ggtggcagtg attaaagatt taacaaaaga acaaaaagcc
    57181 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    57241 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    57301 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    57361 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactgaa
    57421 accgatgccg caaagttggc gcggcgtgct accgtgtgcc gattgcgaag gaatcgaaat
    57481 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    57541 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    57601 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    57661 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    57721 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    57781 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    57841 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    57901 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    57961 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaaacctg
    58021 cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    58081 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    58141 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    58201 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac
    58261 gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    58321 gccagtgtaa gtggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac
    58381 cacgactttt tgtctccttc gcacacactg cttaactgcg ctttcgccag gtcgctgacg
    58441 cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg agtcaccata
    58501 aagggaataa attcgacaat gctgcgatag ttataaccgc tatctttgat tttattggca
    58561 aacgcggtga tatctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    58621 ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    58681 tctgcaacgt tattatcacc cagaaagatt gcaacgtgac tcacagagga agtgctgaag
    58741 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    58801 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    58861 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    58921 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    58981 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    59041 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    59101 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    59161 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    59221 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    59281 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    59341 ctggaacggc gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat
    59401 cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt
    59461 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    59521 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct
    59581 tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc
    59641 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    59701 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    59761 cggaccggct ttaaccacag cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc
    59821 aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag
    59881 cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    59941 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
    60001 aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc
    60061 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    60121 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    60181 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    60241 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    60301 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    60361 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    60421 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    60481 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    60541 ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat
    60601 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    60661 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    60721 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    60781 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    60841 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    60901 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    60961 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    61021 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    61081 tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata gctggcactg
    61141 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    61201 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    61261 ttcagctcta ccagcgacat gccaattggg ttagggcgga aagtagagcg tgttgcgaaa
    61321 acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg ccagccgcct
    61381 tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc caggccgcga
    61441 acggcgtcgg cctggttgta ggaagcaatg agatgcagtt cgccgttggc gctttttacc
    61501 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    61561 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    61621 cctgacgata gcagcccggc gtaccgctgg tgacttcgca gctatgcagt aatacagcat
    61681 tggctttcat tttagaggca ttgatttgca tctgcttacg tgcggttgga atgctcggcg
    61741 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    61801 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    61861 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    61921 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    61981 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    62041 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    62101 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    62161 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    62221 ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg tctttcgccg
    62281 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    62341 cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga ttttttgggt
    62401 tctcaacaac atcaagaact gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    62461 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    62521 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    62581 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    62641 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    62701 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    62761 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    62821 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    62881 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    62941 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    63001 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttgt
    63061 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    63121 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    63181 gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca cccattgcgc
    63241 gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc atacgggcaa
    63301 taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca atcgatgtac
    63361 cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    63421 ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    63481 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac ccaaaaaatc
    63541 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    63601 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    63661 gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa tttaacgcca
    63721 ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    63781 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    63841 cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat atccagatgc
    63901 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    63961 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatacgc
    64021 ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttaatg
    64081 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    64141 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    64201 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    64261 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    64321 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    64381 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa ttcctggcca
    64441 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    64501 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    64561 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    64621 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca tctcgaatgc
    64681 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    64741 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcccatgc
    64801 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    64861 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    64921 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    64981 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    65041 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    65101 catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    65161 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    65221 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    65281 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    65341 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc gcaatgatta
    65401 aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag ccgactccct
    65461 ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag ccggttcggt
    65521 tgaaga
//
LOCUS       DADLXX010000006        65142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLXX010000006 DADLXX010000000
VERSION     DADLXX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.006"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNX27_000378"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNX27_000378"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7881715.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNX27_000379"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNX27_000379"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7881716.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNX27_000380"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNX27_000380"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7881717.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JNX27_000381"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JNX27_000381"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7881718.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPTRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JNX27_000382"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JNX27_000382"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7881719.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JNX27_000383"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JNX27_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7881720.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JNX27_000384"
     CDS             9708..10559
                     /locus_tag="JNX27_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7881721.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JNX27_000385"
     CDS             complement(10546..10887)
                     /locus_tag="JNX27_000385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7881722.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JNX27_000386"
     CDS             complement(10889..11767)
                     /locus_tag="JNX27_000386"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7881723.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCSWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JNX27_000387"
     CDS             complement(11733..14030)
                     /locus_tag="JNX27_000387"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7881724.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQGRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGHDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JNX27_000388"
     CDS             complement(14081..14401)
                     /locus_tag="JNX27_000388"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7881725.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="JNX27_000389"
     CDS             complement(14416..15495)
                     /locus_tag="JNX27_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7881726.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="JNX27_000390"
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="JNX27_000390"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7881727.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="JNX27_000391"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="JNX27_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20082
                     /gene="gldA"
                     /locus_tag="JNX27_000392"
                     /pseudo
     CDS             18990..20082
                     /gene="gldA"
                     /locus_tag="JNX27_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20974
                     /locus_tag="JNX27_000393"
     CDS             20357..20974
                     /locus_tag="JNX27_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7881728.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPDGDVPQECGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21001..21939)
                     /gene="yijE"
                     /locus_tag="JNX27_000394"
     CDS             complement(21001..21939)
                     /gene="yijE"
                     /locus_tag="JNX27_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7881729.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22000..24180)
                     /gene="katG"
                     /locus_tag="JNX27_000395"
     CDS             complement(22000..24180)
                     /gene="katG"
                     /locus_tag="JNX27_000395"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7881730.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24509..25399)
                     /gene="metF"
                     /locus_tag="JNX27_000396"
     CDS             complement(24509..25399)
                     /gene="metF"
                     /locus_tag="JNX27_000396"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7881731.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25748..28180)
                     /locus_tag="JNX27_000397"
     CDS             complement(25748..28180)
                     /locus_tag="JNX27_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7881732.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28183..29343)
                     /gene="metB"
                     /locus_tag="JNX27_000398"
     CDS             complement(28183..29343)
                     /gene="metB"
                     /locus_tag="JNX27_000398"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7881733.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29620..29937
                     /gene="metJ"
                     /locus_tag="JNX27_000399"
     CDS             29620..29937
                     /gene="metJ"
                     /locus_tag="JNX27_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7881734.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30121..30729
                     /locus_tag="JNX27_000400"
     CDS             30121..30729
                     /locus_tag="JNX27_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7881735.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="JNX27_000401"
     CDS             complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="JNX27_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7881736.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31205..33403
                     /gene="priA"
                     /locus_tag="JNX27_000402"
     CDS             31205..33403
                     /gene="priA"
                     /locus_tag="JNX27_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7881737.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33559..34584
                     /gene="cytR"
                     /locus_tag="JNX27_000403"
     CDS             33559..34584
                     /gene="cytR"
                     /locus_tag="JNX27_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7881738.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34676..35635
                     /gene="ftsN"
                     /locus_tag="JNX27_000404"
     CDS             34676..35635
                     /gene="ftsN"
                     /locus_tag="JNX27_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7881739.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35728..36258
                     /gene="hslV"
                     /locus_tag="JNX27_000405"
     CDS             35728..36258
                     /gene="hslV"
                     /locus_tag="JNX27_000405"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7881740.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36268..37599
                     /gene="hslU"
                     /locus_tag="JNX27_000406"
     CDS             36268..37599
                     /gene="hslU"
                     /locus_tag="JNX27_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7881741.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37666..38592
                     /gene="menA"
                     /locus_tag="JNX27_000407"
     CDS             37666..38592
                     /gene="menA"
                     /locus_tag="JNX27_000407"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7881742.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38685..39170
                     /gene="rraA"
                     /locus_tag="JNX27_000408"
     CDS             38685..39170
                     /gene="rraA"
                     /locus_tag="JNX27_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7881743.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="JNX27_000409"
     CDS             complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="JNX27_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7881744.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39926..40771
                     /gene="glpF"
                     /locus_tag="JNX27_000410"
                     /pseudo
     CDS             39926..40771
                     /gene="glpF"
                     /locus_tag="JNX27_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40811..42301
                     /gene="glpK"
                     /locus_tag="JNX27_000411"
     CDS             40811..42301
                     /gene="glpK"
                     /locus_tag="JNX27_000411"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7881745.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42531..43541
                     /gene="glpX"
                     /locus_tag="JNX27_000412"
     CDS             42531..43541
                     /gene="glpX"
                     /locus_tag="JNX27_000412"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7881746.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43638..44384
                     /gene="fpr"
                     /locus_tag="JNX27_000413"
     CDS             43638..44384
                     /gene="fpr"
                     /locus_tag="JNX27_000413"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7881747.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="JNX27_000414"
     CDS             complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="JNX27_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7881748.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44844..45143)
                     /locus_tag="JNX27_000415"
     CDS             complement(44844..45143)
                     /locus_tag="JNX27_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7881749.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAAFSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45355..45795)
                     /locus_tag="JNX27_000416"
                     /pseudo
     CDS             complement(45355..45795)
                     /locus_tag="JNX27_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45896..46495
                     /locus_tag="JNX27_000417"
     CDS             45896..46495
                     /locus_tag="JNX27_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7881750.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46603..47370
                     /gene="tpiA"
                     /locus_tag="JNX27_000418"
     CDS             46603..47370
                     /gene="tpiA"
                     /locus_tag="JNX27_000418"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7881751.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="JNX27_000419"
     CDS             complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="JNX27_000419"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7881752.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48287..49276)
                     /locus_tag="JNX27_000420"
     CDS             complement(48287..49276)
                     /locus_tag="JNX27_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7881753.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="JNX27_000421"
     CDS             complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="JNX27_000421"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7881754.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="JNX27_000422"
     CDS             complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="JNX27_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7881755.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="JNX27_000423"
     CDS             complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="JNX27_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7881756.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52439..53137
                     /gene="cpxR"
                     /locus_tag="JNX27_000424"
     CDS             52439..53137
                     /gene="cpxR"
                     /locus_tag="JNX27_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7881757.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53134..54507
                     /gene="cpxA"
                     /locus_tag="JNX27_000425"
     CDS             53134..54507
                     /gene="cpxA"
                     /locus_tag="JNX27_000425"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7881758.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="JNX27_000426"
     CDS             complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="JNX27_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7881759.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="JNX27_000427"
     CDS             complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="JNX27_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7881760.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="JNX27_000428"
     CDS             complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="JNX27_000428"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7881761.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57584..58618
                     /gene="rhaT"
                     /locus_tag="JNX27_000429"
                     /pseudo
     CDS             57584..58618
                     /gene="rhaT"
                     /locus_tag="JNX27_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58615..59552)
                     /gene="rhaR"
                     /locus_tag="JNX27_000430"
                     /pseudo
     CDS             complement(58615..59552)
                     /gene="rhaR"
                     /locus_tag="JNX27_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="JNX27_000431"
                     /pseudo
     CDS             complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="JNX27_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            60660..62129
                     /gene="rhaB"
                     /locus_tag="JNX27_000432"
     CDS             60660..62129
                     /gene="rhaB"
                     /locus_tag="JNX27_000432"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7881762.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62126..63385
                     /gene="rhaA"
                     /locus_tag="JNX27_000433"
     CDS             62126..63385
                     /gene="rhaA"
                     /locus_tag="JNX27_000433"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7881763.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63627..64451
                     /gene="rhaD"
                     /locus_tag="JNX27_000434"
     CDS             63627..64451
                     /gene="rhaD"
                     /locus_tag="JNX27_000434"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY7881764.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            64461..64775
                     /gene="rhaM"
                     /locus_tag="JNX27_000435"
     CDS             64461..64775
                     /gene="rhaM"
                     /locus_tag="JNX27_000435"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7881765.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            65076..>65142
                     /locus_tag="JNX27_000436"
     CDS             65076..>65142
                     /locus_tag="JNX27_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY7881766.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        15882 a        17551 c        16665 g        15044 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gccaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccaacacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcacca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctaggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttcct ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atagcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgcgcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac attttgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgagcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc agcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cgtgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggccctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccaatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggagtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gccgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaag agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcctag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgtgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcagtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctctaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20521 ggcgtatatg cagcttacct atcctgacgg tgatgttccg caagaatgcg gtgtatgttc
    20581 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20641 agatatggcg aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag
    20701 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20761 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20821 agataacggg ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc
    20881 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20941 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    21001 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21061 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21121 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21181 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21241 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21301 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21361 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21421 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21481 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21541 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21601 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21661 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21721 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21781 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21841 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21901 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21961 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    22021 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22081 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22141 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22201 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22261 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22321 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22381 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22441 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22501 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22561 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22621 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22681 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg acaccaccgc gtttgtcgcc
    22741 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22801 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22861 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22921 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22981 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    23041 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23101 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23161 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23221 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23281 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23341 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23401 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23461 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23521 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23581 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23641 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23701 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23761 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23821 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23881 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23941 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    24001 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24061 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24121 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24181 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24241 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24301 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24361 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24421 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24481 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24541 aaatcgcgta actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24601 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24661 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24721 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24781 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24841 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24901 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24961 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    25021 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacaaata ccgttattcc
    25081 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25141 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25201 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25261 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25321 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25381 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25441 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25501 ccgaaaaatg accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25561 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25621 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25681 tcaatatatt tcattagaaa ccatagcccc gaaaacatga gtaccgggca ttattaaatt
    25741 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25801 cgtcgcgccc agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25861 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25921 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25981 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    26041 tttcaaagaa atggtcgatg ctgccacctt cgcaatgagc aggcaccagc gattccacac
    26101 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26161 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26221 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26281 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26341 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26401 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26461 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26521 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26581 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26641 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26701 gcacaaattc aaagccagta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26761 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26821 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26881 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26941 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    27001 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27061 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27121 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27181 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27241 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27301 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27361 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27421 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27481 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27541 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27601 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27661 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27721 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27781 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27841 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27901 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27961 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    28021 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28081 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28141 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28201 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28261 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28321 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28381 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28441 actcaacatt gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28501 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28561 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28621 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28681 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28741 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28801 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28861 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28921 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac
    28981 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    29041 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29101 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29161 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29221 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29281 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29341 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29401 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29461 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29521 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag taccaacata aattgtgggg
    29581 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29641 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29701 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29761 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29821 gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29881 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29941 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    30001 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30061 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30121 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag
    30181 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30241 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30301 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30361 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30421 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30481 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30541 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30601 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30661 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30721 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30781 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30841 accggtagca acatcacgct gtttgccggt gaagaacggg tggcacttgc tgcacacgtc
    30901 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30961 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    31021 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31081 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31141 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31201 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31261 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31321 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31381 gctaaaagcg gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg
    31441 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31501 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31561 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31621 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31681 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31741 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31801 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31861 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31921 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31981 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    32041 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32101 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32161 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32221 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32281 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
    32341 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32401 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32461 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32521 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32581 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32641 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32701 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32761 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32821 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32881 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32941 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    33001 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33061 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33121 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33181 gatcctctcc agtccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33241 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33301 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33361 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33421 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33481 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33541 accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33601 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33661 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33721 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33781 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33841 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33901 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33961 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    34021 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34081 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34141 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga
    34201 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34261 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34321 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34381 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34441 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34501 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34561 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34621 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34681 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34741 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34801 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    34861 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34921 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34981 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    35041 tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35101 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35161 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35221 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35281 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35341 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    35401 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35461 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35521 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35581 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35641 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35701 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35761 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35821 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35881 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35941 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    36001 cttgaagcac tgctggcagt cgctgatgaa actgcatcgc ttatcatcac cggtaacggt
    36061 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36121 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36181 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36241 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36301 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36361 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36421 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36481 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36541 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36601 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    36661 ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36721 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36781 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36841 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36901 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36961 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    37021 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37081 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37141 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37201 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37261 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37321 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37381 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37441 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37501 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37561 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37621 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37681 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37741 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37801 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37861 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37921 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37981 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    38041 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38101 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38161 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38221 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38281 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38341 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38401 ttttcgctgc atagcctgtg gggctggctg tttctgctgg cggcaccatt actggtgaag
    38461 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38521 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38581 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38641 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    38701 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38761 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38821 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    38881 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38941 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    39001 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39061 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39121 ccgacaatac cgggattatt ctttcagaag atccgctaga tattgaatga taagaaaggc
    39181 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39241 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39301 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39361 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39421 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttatcaaac
    39481 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39541 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39601 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39661 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39721 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39781 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39841 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39901 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39961 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    40021 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40081 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40141 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40201 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40261 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40321 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40381 gatggtgatt accgctattc tgatggggct tatcctggcg ttaacggacg atggcaacgg
    40441 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40501 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40561 ctttgcctag ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40621 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40681 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac
    40741 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40801 aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga
    40861 tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc
    40921 aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga
    40981 agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca
    41041 gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt
    41101 ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga
    41161 ttatatcagc agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa
    41221 gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt
    41281 tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga
    41341 ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa
    41401 aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga
    41461 agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat
    41521 cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa
    41581 gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc
    41641 agaaaatggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt
    41701 ggaaggtgcg gtgtttatgg caggcacatc cattcagtgg ctgcgcgatg agatgaagtt
    41761 gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt
    41821 gtatgtggtt ccggcattta ccgggctgag tgcgccgtac tgggacccgt atgcgcgcgg
    41881 ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga
    41941 gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg
    42001 tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca
    42061 gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg
    42121 tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga
    42181 gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg
    42241 ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta
    42301 atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct
    42361 tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc
    42421 cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac
    42481 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    42541 aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt
    42601 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    42661 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    42721 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    42781 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    42841 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    42901 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    42961 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta
    43021 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    43081 ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    43141 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    43201 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    43261 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    43321 gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    43381 gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg
    43441 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    43501 tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc
    43561 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat
    43621 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    43681 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    43741 aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    43801 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    43861 taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag
    43921 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    43981 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    44041 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    44101 tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg
    44161 aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg
    44221 aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    44281 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt
    44341 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    44401 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    44461 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    44521 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    44581 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    44641 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc
    44701 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac
    44761 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    44821 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    44881 atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    44941 ttaatcttat aggccacgct ggccccttca tctgcagaga atgcagcggc ggcaagctct
    45001 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    45061 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    45121 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    45181 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    45241 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    45301 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggtcaatt
    45361 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    45421 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    45481 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag
    45541 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    45601 gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt
    45661 ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    45721 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    45781 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    45841 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    45901 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    45961 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    46021 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact
    46081 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    46141 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    46201 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    46261 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    46321 aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga
    46381 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    46441 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    46501 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    46561 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    46621 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    46681 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    46741 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    46801 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    46861 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    46921 gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc
    46981 atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag
    47041 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttat
    47101 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    47161 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    47221 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac
    47281 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    47341 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    47401 acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc
    47461 ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga
    47521 attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    47581 cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    47641 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    47701 gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat
    47761 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    47821 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    47881 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    47941 tgacatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt
    48001 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    48061 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    48121 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    48181 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    48241 acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga
    48301 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga
    48361 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta
    48421 cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    48481 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    48541 cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    48601 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat
    48661 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    48721 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    48781 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    48841 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    48901 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    48961 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    49021 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    49081 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    49141 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    49201 taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta
    49261 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    49321 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    49381 gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca
    49441 tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg
    49501 cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt
    49561 ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc
    49621 agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga
    49681 accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg
    49741 atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg
    49801 cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa
    49861 tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta
    49921 cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc
    49981 ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa
    50041 taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga
    50101 cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg
    50161 cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc
    50221 agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca
    50281 cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa
    50341 cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct
    50401 agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc
    50461 agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca
    50521 tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat
    50581 gcaaaatgaa atctgttgcc ggaaatcttc ttgcacatcg aagtgatcca acgaatgtgc
    50641 aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt
    50701 tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta
    50761 ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa
    50821 cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga
    50881 gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga
    50941 agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt
    51001 tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac
    51061 gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag
    51121 ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg
    51181 taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga
    51241 aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct
    51301 ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt
    51361 gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga
    51421 ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc
    51481 aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg
    51541 gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca
    51601 atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac
    51661 tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg
    51721 ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    51781 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    51841 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    51901 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    51961 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    52021 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    52081 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    52141 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    52201 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    52261 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    52321 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    52381 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    52441 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    52501 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    52561 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    52621 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    52681 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    52741 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    52801 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    52861 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga
    52921 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    52981 aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat
    53041 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    53101 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    53161 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    53221 ctcgattcac gccagatgac caagcttctg gatagcgaac agcgtcaggg gctgatgatt
    53281 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    53341 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    53401 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    53461 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    53521 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    53581 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    53641 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    53701 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    53761 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    53821 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    53881 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt
    53941 gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    54001 gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    54061 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    54121 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    54181 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    54241 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    54301 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    54361 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    54421 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    54481 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    54541 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    54601 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    54661 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    54721 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    54781 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    54841 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    54901 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    54961 tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt
    55021 tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    55081 cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    55141 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    55201 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    55261 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    55321 accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca
    55381 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    55441 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    55501 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    55561 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    55621 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    55681 atttatcggc gataatcaac ggaataccgg tcacgataat tattgccaca cccaacagga
    55741 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    55801 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    55861 gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    55921 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    55981 acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc atgatggaag
    56041 cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    56101 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    56161 cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg
    56221 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    56281 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    56341 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    56401 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa
    56461 ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    56521 agtgatcaaa accacatcaa ttgaaacgct gttttatttt tataggatca ttgtttttat
    56581 tttggaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    56641 tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt
    56701 gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc cggacggcgg
    56761 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    56821 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    56881 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    56941 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    57001 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    57061 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    57121 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    57181 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    57241 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    57301 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggtttatta
    57361 tagttaatta aatgatattg aaaatgatta tcaatgccgc acttttcgta agggtatggt
    57421 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    57481 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    57541 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    57601 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    57661 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    57721 gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg cgtattacag
    57781 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    57841 taatatcaac tatggcctga ccatgcgtta cctcggcatg tcgatgggaa ttggcatcgc
    57901 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    57961 tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg tggcgctgat
    58021 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc
    58081 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    58141 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg
    58201 cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat
    58261 cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    58321 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg
    58381 gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca ttccggcgca
    58441 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    58501 cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg tgttgagcct
    58561 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    58621 tttctgcgaa ttgagatgcg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    58681 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    58741 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
    58801 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    58861 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
    58921 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    58981 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
    59041 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    59101 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    59161 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    59221 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    59281 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    59341 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    59401 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    59461 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    59521 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    59581 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    59641 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcagg ctttcatcag
    59701 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    59761 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    59821 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    59881 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    59941 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    60001 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    60061 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    60121 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
    60181 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    60241 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    60301 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg
    60361 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    60421 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    60481 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
    60541 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    60601 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    60661 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    60721 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    60781 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
    60841 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    60901 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    60961 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
    61021 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
    61081 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    61141 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
    61201 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    61261 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    61321 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    61381 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
    61441 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    61501 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    61561 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
    61621 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    61681 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
    61741 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
    61801 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
    61861 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
    61921 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaattacgc
    61981 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    62041 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
    62101 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    62161 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
    62221 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    62281 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
    62341 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    62401 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
    62461 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    62521 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    62581 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    62641 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    62701 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    62761 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    62821 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    62881 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
    62941 gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg
    63001 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
    63061 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
    63121 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    63181 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    63241 tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    63301 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    63361 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc
    63421 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
    63481 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    63541 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    63601 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    63661 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    63721 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
    63781 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    63841 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
    63901 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
    63961 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    64021 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    64081 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
    64141 gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    64201 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    64261 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
    64321 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    64381 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
    64441 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    64501 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
    64561 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    64621 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
    64681 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
    64741 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg gagatgcctc
    64801 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    64861 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    64921 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    64981 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    65041 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    65101 ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DADLXX010000007        62064 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLXX010000007 DADLXX010000000
VERSION     DADLXX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.007"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            358..444
                     /gene="leuL"
                     /locus_tag="JNX27_000437"
     CDS             358..444
                     /gene="leuL"
                     /locus_tag="JNX27_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7881767.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            537..2108
                     /gene="leuA"
                     /locus_tag="JNX27_000438"
     CDS             537..2108
                     /gene="leuA"
                     /locus_tag="JNX27_000438"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7881768.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2108..3199
                     /gene="leuB"
                     /locus_tag="JNX27_000439"
     CDS             2108..3199
                     /gene="leuB"
                     /locus_tag="JNX27_000439"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7881769.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3202..4602
                     /gene="leuC"
                     /locus_tag="JNX27_000440"
     CDS             3202..4602
                     /gene="leuC"
                     /locus_tag="JNX27_000440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7881770.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4613..5218
                     /gene="leuD"
                     /locus_tag="JNX27_000441"
     CDS             4613..5218
                     /gene="leuD"
                     /locus_tag="JNX27_000441"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7881771.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5267..6445)
                     /gene="setA"
                     /locus_tag="JNX27_000442"
     CDS             complement(5267..6445)
                     /gene="setA"
                     /locus_tag="JNX27_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY7881772.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6547..6678)
                     /gene="sgrT"
                     /locus_tag="JNX27_000443"
     CDS             complement(6547..6678)
                     /gene="sgrT"
                     /locus_tag="JNX27_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7881773.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6767..8422
                     /locus_tag="JNX27_000444"
     CDS             6767..8422
                     /locus_tag="JNX27_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7881774.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8456..8555
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8586..9569
                     /gene="thiB"
                     /locus_tag="JNX27_000445"
     CDS             8586..9569
                     /gene="thiB"
                     /locus_tag="JNX27_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7881775.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9545..11155
                     /gene="thiP"
                     /locus_tag="JNX27_000446"
     CDS             9545..11155
                     /gene="thiP"
                     /locus_tag="JNX27_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY7881776.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11139..11837
                     /gene="thiQ"
                     /locus_tag="JNX27_000447"
     CDS             11139..11837
                     /gene="thiQ"
                     /locus_tag="JNX27_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7881777.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11951..12715)
                     /locus_tag="JNX27_000448"
     CDS             complement(11951..12715)
                     /locus_tag="JNX27_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7881778.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12801..13679)
                     /gene="araC"
                     /locus_tag="JNX27_000449"
     CDS             complement(12801..13679)
                     /gene="araC"
                     /locus_tag="JNX27_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7881779.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14018..15717
                     /gene="araB"
                     /locus_tag="JNX27_000450"
                     /pseudo
     CDS             14018..15717
                     /gene="araB"
                     /locus_tag="JNX27_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            15728..17230
                     /gene="araA"
                     /locus_tag="JNX27_000451"
     CDS             15728..17230
                     /gene="araA"
                     /locus_tag="JNX27_000451"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7881780.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17430..18125
                     /gene="araD"
                     /locus_tag="JNX27_000452"
     CDS             17430..18125
                     /gene="araD"
                     /locus_tag="JNX27_000452"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7881781.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSDVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18200..20551
                     /gene="polB"
                     /locus_tag="JNX27_000453"
     CDS             18200..20551
                     /gene="polB"
                     /locus_tag="JNX27_000453"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7881782.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20716..23622
                     /gene="rapA"
                     /locus_tag="JNX27_000454"
     CDS             20716..23622
                     /gene="rapA"
                     /locus_tag="JNX27_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7881783.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23634..24293
                     /gene="rluA"
                     /locus_tag="JNX27_000455"
     CDS             23634..24293
                     /gene="rluA"
                     /locus_tag="JNX27_000455"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7881784.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24410..25225)
                     /gene="djlA"
                     /locus_tag="JNX27_000456"
     CDS             complement(24410..25225)
                     /gene="djlA"
                     /locus_tag="JNX27_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7881785.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25480..27834
                     /gene="lptD"
                     /locus_tag="JNX27_000457"
     CDS             25480..27834
                     /gene="lptD"
                     /locus_tag="JNX27_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7881786.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTYVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27887..29173
                     /gene="surA"
                     /locus_tag="JNX27_000458"
     CDS             27887..29173
                     /gene="surA"
                     /locus_tag="JNX27_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7881787.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKLSEEAASWMQEQRASAYVKILSN"
     gene            29173..30162
                     /gene="pdxA"
                     /locus_tag="JNX27_000459"
     CDS             29173..30162
                     /gene="pdxA"
                     /locus_tag="JNX27_000459"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7881788.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGITEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30159..30980
                     /gene="rsmA"
                     /locus_tag="JNX27_000460"
     CDS             30159..30980
                     /gene="rsmA"
                     /locus_tag="JNX27_000460"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7881789.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30983..31360
                     /gene="apaG"
                     /locus_tag="JNX27_000461"
     CDS             30983..31360
                     /gene="apaG"
                     /locus_tag="JNX27_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7881790.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31367..32209
                     /gene="apaH"
                     /locus_tag="JNX27_000462"
     CDS             31367..32209
                     /gene="apaH"
                     /locus_tag="JNX27_000462"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7881791.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32287..32766)
                     /gene="folA"
                     /locus_tag="JNX27_000463"
     CDS             complement(32287..32766)
                     /gene="folA"
                     /locus_tag="JNX27_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7881792.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32957..34819)
                     /gene="kefC"
                     /locus_tag="JNX27_000464"
     CDS             complement(32957..34819)
                     /gene="kefC"
                     /locus_tag="JNX27_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY7881793.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34812..35342)
                     /gene="kefF"
                     /locus_tag="JNX27_000465"
     CDS             complement(34812..35342)
                     /gene="kefF"
                     /locus_tag="JNX27_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7881794.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35390..36784)
                     /locus_tag="JNX27_000466"
     CDS             complement(35390..36784)
                     /locus_tag="JNX27_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7881795.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36843..37130)
                     /gene="fixX"
                     /locus_tag="JNX27_000467"
     CDS             complement(36843..37130)
                     /gene="fixX"
                     /locus_tag="JNX27_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY7881796.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37127..38412)
                     /locus_tag="JNX27_000468"
                     /pseudo
     CDS             complement(37127..38412)
                     /locus_tag="JNX27_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(38463..39404)
                     /locus_tag="JNX27_000469"
     CDS             complement(38463..39404)
                     /locus_tag="JNX27_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7881797.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQTRQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVRISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39419..40189)
                     /locus_tag="JNX27_000470"
     CDS             complement(39419..40189)
                     /locus_tag="JNX27_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY7881798.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40660..42174
                     /locus_tag="JNX27_000471"
     CDS             40660..42174
                     /locus_tag="JNX27_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAY7881799.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42205..43347
                     /gene="caiA"
                     /locus_tag="JNX27_000472"
     CDS             42205..43347
                     /gene="caiA"
                     /locus_tag="JNX27_000472"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY7881800.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43476..44693
                     /gene="caiB"
                     /locus_tag="JNX27_000473"
     CDS             43476..44693
                     /gene="caiB"
                     /locus_tag="JNX27_000473"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY7881801.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44767..46320
                     /gene="caiC"
                     /locus_tag="JNX27_000474"
     CDS             44767..46320
                     /gene="caiC"
                     /locus_tag="JNX27_000474"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY7881802.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46429..47214
                     /gene="caiD"
                     /locus_tag="JNX27_000475"
     CDS             46429..47214
                     /gene="caiD"
                     /locus_tag="JNX27_000475"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY7881803.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47220..47810
                     /gene="caiE"
                     /locus_tag="JNX27_000476"
     CDS             47220..47810
                     /gene="caiE"
                     /locus_tag="JNX27_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY7881804.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47896..48291)
                     /gene="caiF"
                     /locus_tag="JNX27_000477"
     CDS             complement(47896..48291)
                     /gene="caiF"
                     /locus_tag="JNX27_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY7881805.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48549..51770)
                     /gene="carB"
                     /locus_tag="JNX27_000478"
     CDS             complement(48549..51770)
                     /gene="carB"
                     /locus_tag="JNX27_000478"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7881806.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51788..52936)
                     /gene="carA"
                     /locus_tag="JNX27_000479"
     CDS             complement(51788..52936)
                     /gene="carA"
                     /locus_tag="JNX27_000479"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY7881807.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(53392..54213)
                     /gene="dapB"
                     /locus_tag="JNX27_000480"
     CDS             complement(53392..54213)
                     /gene="dapB"
                     /locus_tag="JNX27_000480"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7881808.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(54380..55294)
                     /gene="rihC"
                     /locus_tag="JNX27_000481"
     CDS             complement(54380..55294)
                     /gene="rihC"
                     /locus_tag="JNX27_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7881809.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(55360..56310)
                     /gene="ispH"
                     /locus_tag="JNX27_000482"
                     /gene_synonym="lytB"
     CDS             complement(55360..56310)
                     /gene="ispH"
                     /locus_tag="JNX27_000482"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7881810.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(56312..56761)
                     /gene="fkpB"
                     /locus_tag="JNX27_000483"
     CDS             complement(56312..56761)
                     /gene="fkpB"
                     /locus_tag="JNX27_000483"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7881811.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(56848..57342)
                     /gene="lspA"
                     /locus_tag="JNX27_000484"
     CDS             complement(56848..57342)
                     /gene="lspA"
                     /locus_tag="JNX27_000484"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7881812.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(57342..60158)
                     /gene="ileS"
                     /locus_tag="JNX27_000485"
     CDS             complement(57342..60158)
                     /gene="ileS"
                     /locus_tag="JNX27_000485"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7881813.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(60201..61142)
                     /gene="ribF"
                     /locus_tag="JNX27_000486"
     CDS             complement(60201..61142)
                     /gene="ribF"
                     /locus_tag="JNX27_000486"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7881814.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(61150..61368)
                     /locus_tag="JNX27_000487"
     CDS             complement(61150..61368)
                     /locus_tag="JNX27_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7881815.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            61471..61734
                     /gene="rpsT"
                     /locus_tag="JNX27_000488"
     CDS             61471..61734
                     /gene="rpsT"
                     /locus_tag="JNX27_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7881816.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        14935 a        16940 c        16136 g        14053 t
ORIGIN      
        1 tgcattaatt cttaacatta attgatcaat aatattcacc aaatcaatat caaaaaaaaa
       61 tcgcaaaaca tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg
      121 tccccttttt cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa
      181 aagcgtcggt agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag
      241 ctaattttac ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca
      301 tccgtttttg tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg
      361 actcacatcg ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga
      421 cgagtgagcg gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg
      481 cgcgggtttt tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga
      541 gccagcaagt cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa
      601 gcttgagtgt gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg
      661 tgatggaagt cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg
      721 cccgccaggt taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg
      781 acgtggcggc cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta
      841 cctccccaat gcacatcgcc accaagctgc gcagcaccct ggacgaggtg atcgaacgcg
      901 ctatctatat ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag
      961 atgccgggcg tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg
     1021 gtgccaccac catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg
     1081 gaatcatcag cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac
     1141 atacccacga cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg
     1201 cacgccaggt ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg
     1261 aagaagtcat catggcgatt aaagttcgta aggatattct caacgtccac accgccatta
     1321 atcaccagga gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc
     1381 cggcaaacaa agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg
     1441 atggcgtgct gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga
     1501 accaaatcca gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg
     1561 atgagatggg gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga
     1621 agctggcgga caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg
     1681 gtaagcagca agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct
     1741 ctaacgatat cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag
     1801 ccgccaacgg taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact
     1861 ataacgtcga actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg
     1921 gtcaggtgga tattgtcgcc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca
     1981 ccgatattgt cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg
     2041 ccgcagaagt cgaaaaagag ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa
     2101 ccgtgtgatg tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga
     2161 agtgatgacc caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat
     2221 caccaccagc cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc
     2281 gcctgcgacg gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg
     2341 cccgaagtgg gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct
     2401 gcgtaagcac ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga
     2461 agcattctgt ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc tgtgcgtgcg
     2521 cgaactgacc ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata
     2581 tgaaaaagcg tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat
     2641 cgcgtttgaa tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt
     2701 gctgcaatcc tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga
     2761 tgtcgaactg gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc
     2821 acagtttgac gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc
     2881 aatgatcact ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg
     2941 actgtatgaa ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc
     3001 gattgcacaa atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc
     3061 ggcttccgcc attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga
     3121 tttagcccgt ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta
     3181 tgtggcagaa ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac
     3241 gtagtgtacg aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat
     3301 gaagtgacct caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag
     3361 ccgggcaaaa ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat
     3421 gcctgtggtg aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt
     3481 ggcgtcgagc tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg
     3541 gaacagggcg tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc
     3601 cacggcgcgt ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg
     3661 gcaacacaaa ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa
     3721 gccgcgccgg gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc
     3781 gcaggcggca ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg
     3841 gaaggtcgta tgaccctgtg caatatggcg atcgaaatgg gcgcaaaatc cggtctggtt
     3901 gctcctgacg aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa
     3961 gatttcgacg acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc
     4021 gataccgttg tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat
     4081 cccggccagg tgatttccgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg
     4141 gttgaacgtg cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg
     4201 ctgaccgaag tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa
     4261 gatttacgcg cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca
     4321 ctggtggttc ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa
     4381 atatttattg aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg
     4441 aacaacgacc gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa
     4501 ggccgccagg ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct
     4561 gctgtgaccg gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga
     4621 gaaatttatc aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga
     4681 tgcaatcatc ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct
     4741 gtttaacgac tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct
     4801 gaacttcccg cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg
     4861 ctcctcgcgt gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc
     4921 gccgagtttt gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa
     4981 attaagcgat gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca
     5041 tttcgacgtg gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac
     5101 catcgatgcc ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct
     5161 gcagcacgac gacgcgattg cgtcttatga agcaaaacaa cctgcgttta tgaattagcc
     5221 cccttgcccg gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa
     5281 cctttgcggt taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa
     5341 agtgccccca actttgtgca attgctccct gaataacacc agccagaatt accccggtag
     5401 agatagtgtt ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa
     5461 accatagcat cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg
     5521 tcatcaacgc catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca
     5581 ccactgcaat gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa
     5641 tcattgctgg tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg
     5701 gcaatcctaa atcgctactg atccacaacg gcatatcaat aatgtacata gtgttgcagg
     5761 tccacattaa cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt
     5821 gcattgataa agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg
     5881 caatcaatac cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc
     5941 cgtaattcaa cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct
     6001 gcgcacgcat caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc
     6061 gcgccagagc aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac
     6121 aggtgataag cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca
     6181 tcaaacagca aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca
     6241 accagaggct tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga
     6301 aaggttgcgc gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc
     6361 cggccacccc catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc
     6421 gagccatcgt cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa
     6481 ccagcaggta taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca
     6541 ggcaagtcaa ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa
     6601 tcgttgtggg acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg
     6661 ctgataaaac tgacgcatgg ggcatctcct tgcttcatcg ttaggaatta ttgacttaat
     6721 atagggaaaa taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg
     6781 tctgcaacaa cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac
     6841 gctcaacgaa ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa
     6901 caccatgcag gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc
     6961 gcgtctgaca ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct
     7021 ggagcaggat cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca
     7081 aatgctggtt tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct
     7141 ctactatcgt ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca
     7201 tattgcccgg caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga
     7261 agcagacatc gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg
     7321 tccaggagtc cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt
     7381 aaaacgaatc aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc
     7441 ctggacgctg gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca
     7501 agttccggcg atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc
     7561 tattggcacc ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc
     7621 attcgatgac ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc
     7681 ggaaattgcc gacgagccag ccggtgatct gatgctaaaa ggtccacagg gcgaggaaaa
     7741 agagattgaa agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca
     7801 tcgcggggcg aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct
     7861 ggtctatttc gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc
     7921 ccgttggcac catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac
     7981 cctgaccttt tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat
     8041 tctggcaagt caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga
     8101 aggagagatc gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt
     8161 ttcgctgttc gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg
     8221 gcaagccgac gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca
     8281 actggtcgcc agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca
     8341 acgcagtatg cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg
     8401 gtttgcgcca ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt
     8461 ctcaacgggg tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga
     8521 taacgccggc gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt
     8581 gcaaagtgtt aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta
     8641 aacccgttct gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa
     8701 agatcaaaaa agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag
     8761 atggcgtttc gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg
     8821 tgctggggct ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa
     8881 gcggtgtggc agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc
     8941 cgtttgatta cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc
     9001 aaagcctgaa agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc
     9061 gtaccagcac gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg
     9121 ccccacaagc ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg
     9181 aagcctacgg cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc
     9241 cggcttatca cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag
     9301 gtcactatct gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg
     9361 cgcaaaaatt cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta
     9421 actggatgta tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac
     9481 cggccaccac gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta
     9541 gcgaatggca acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc
     9601 cgccacgctg gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc
     9661 gcagggtaac tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc
     9721 cttctggcag gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg
     9781 cgccctctat cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac
     9841 cttgatcctc ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg
     9901 ctggctggca tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct
     9961 gcaaggcatt ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact
    10021 ccaggcactg gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg
    10081 cggctggcat tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt
    10141 tgccgcgctt atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg
    10201 cggtccgcag gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga
    10261 tcctgcccgc gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct
    10321 gttgagtcag cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg
    10381 cgatccggac gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct
    10441 gctgctgttg ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc
    10501 ggaagtgctg gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct
    10561 ggcggcaggt gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact
    10621 gcgggcgcgg cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct
    10681 cgccatgccg gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg
    10741 cctgccacaa tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta
    10801 tgcgctgaaa gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg
    10861 tcagtcgctg ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa
    10921 acgtccactg gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt
    10981 agtggcgttg ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat
    11041 tggctcctat cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt
    11101 tctgctgttt accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca
    11161 cctggcttta tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg
    11221 tggcaatcct cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt
    11281 ttctgacgcc agccagcggt tcgctgacta tcgatggcgt tgatcacacc acgacgccgc
    11341 cgtcacgccg tccggtgtcg atgctgtttc aggagaacaa cctgttcagc cacctgacgg
    11401 tcgcgcagaa catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg
    11461 agaaaatgca cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg
    11521 gcgagctttc cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac
    11581 agccgatttt attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga
    11641 tgctgacgct ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc
    11701 acagtgtgga agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca
    11761 tcgtctggca gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat
    11821 tggggattac gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg
    11881 gacagtgcgt tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca
    11941 agacaatccc ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg
    12001 ggtggcgaat taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc
    12061 acaacaaacg gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc
    12121 ataaccgcca gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc
    12181 atttaaactc gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc
    12241 caggcaggaa gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt
    12301 ttgccaccgg cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa
    12361 caaagcgacc gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat
    12421 cgagtaatgc tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc
    12481 agcccagcca gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag
    12541 cgtgccagaa acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg
    12601 taccgggtag aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg
    12661 ccatcaatga atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt
    12721 gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca
    12781 attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca
    12841 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa
    12901 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg
    12961 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc
    13021 cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg
    13081 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg
    13141 cgtacacgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt
    13201 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg
    13261 gcgttaatga tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga
    13321 aagaaacccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc
    13381 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga
    13441 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc
    13501 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc
    13561 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc
    13621 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata
    13681 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
    13741 cgtcactgcg tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa
    13801 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
    13861 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
    13921 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
    13981 gtttctccat acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga
    14041 ttttggcagt gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc
    14101 caccagcgta gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa
    14161 ccagttccgt catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt
    14221 gcttgcagag cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac
    14281 cggctcgacg cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt
    14341 tgccgaaaac ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc
    14401 ggaagagatt acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg
    14461 cggtatttat tccagcgaag gttctgggca aaaatcctgc atgtgactcg ccaggacagc
    14521 gccgtggcgc aatctgccgc atcgtggatt gagctgtgcg actgggtgcc agctctgctt
    14581 tccggtacca cccgcccgca ggatattcgt cgcggacgtt gcagcgccgg gcataaatct
    14641 ctgtggcacg aaagctgggg cggcttgccg ccagccagtt tctttgatga gctggacccg
    14701 atcctcaatc gccatttgcc ttccccgctg ttcactgaca cctggactgc cgatattccg
    14761 gtgggcacct tatgcccgga atgggcgcag cgtctcggcc tgcctgaaag cgtggtgatt
    14821 tccggcggcg cgtttgactg ccatatgggc gcagttggcg caggcgcaca gcctaacgca
    14881 ctggtaaaag ttatcggtac ttccacctgc gacattctga ttgccgacaa acagagcgtt
    14941 ggcgagcggg cagttaaagg tatttgcggt caggttgatg gcagcgtggt gcctggattt
    15001 atcggtctgg aagcaggcca atcggcgttt ggtgatatct acgcctggtt tggtcgcgta
    15061 ctcggctggc cgctggaaca gcttgccgcc cagcatgcgg aactgaaagc gcaaatcaac
    15121 gccagccaga aacaactgct tccggcgctg accgaagcat gggccaaaaa tccgtctctg
    15181 gatcacctgc cggtggtgct cgactggttt aacggtcgtc gcacgccaaa cgctaaccaa
    15241 cgcctgaaag gggtgattac cgatcttaac ctcgctaccg acactccgct gctgttcggc
    15301 ggtttgattg ctgccaccgc ctttggcgca cgcgcaatca tggagtgctt taccgatcag
    15361 gggatcgccg tcaataacgt gatggcgctg ggcggcatcg cgcggaaaaa ccaggtcatt
    15421 atgcaggcct gctgcgacgt gctgaatcgc ccgctgcaaa ttgttgcctc tgaccagtgc
    15481 tgtgcgctcg gtgcggcgat ttttgctgcc gtcgccgcga aagtgcacgc agacatccca
    15541 tcagcccagc aaaaaatggc cagtgcggta gagaaaaccc tgcaaccgcg cagcgaacag
    15601 gcacaacgct ttgaacagct ttatcgccgc tatcagcaat gggcgatgag cgccgaacaa
    15661 cactatcttc caacttccgc cccggcacag gctgcccagg ccgttgcgac tctataagga
    15721 cacgataatg acgatttttg ataattatga agtgtggttt gtcattggca gccagcatct
    15781 gtatggcccg gaaaccctgc gtcaggtcac ccaacatgcc gagcacgttg ttaatgcgct
    15841 gaatacggaa gcgaaactgc cctgcaaact ggtgttgaaa ccgctgggca ccacgccgga
    15901 tgaaatcacc gctatttgcc gcgacgcgaa ttacgacgat cgttgcgctg gtctggtggt
    15961 gtggctgcac accttctccc cggccaaaat gtggatcaac ggcctgacca tgctcaacaa
    16021 accgttgctg caattccaca cccagttcaa cgcggcgctg ccgtgggaca gtatcgatat
    16081 ggactttatg aacctgaacc agactgcaca tggcggtcgc gagttcggct tcattggcgc
    16141 gcgtatgcgt cagcaacatg ccgtcgttac cggtcactgg caggataaac aagcccatga
    16201 gcgtatcggc tcctggatgc gtcaggcggt ctctaaacag gatacccgtc atctgaaagt
    16261 ctgccgattt ggcgataaca tgcgtgaagt ggcggtcacc gatggcgata aagttgccgc
    16321 acagatcaag ttcggtttct ccgtcaatac ctgggcggtt ggcgatctgg tgcaggtggt
    16381 gaactccatc agcgacggcg atgttaacgc gctggtcgat gagtacgaaa gctgctacac
    16441 catgacgcct gccacacaaa tccacggcga aaaacgacag aacgtgctgg aagcggcgcg
    16501 tattgagctg gggatgaagc gtttcctgga acaaggtggc ttccacgcgt tcaccaccac
    16561 ctttgaagat ttgcacggtc tgaaacagct tccaggtctg cccgtacagc gtctgatgca
    16621 gcagggttac ggctttgcgg gcgaaggcga ctggaaaacc gccgccctgc ttcgcatcat
    16681 gaaggtgatg tcaaccggtc tgcagggcgg cacctccttt atggaggact acacctatca
    16741 cttcgagaaa ggtaatgacc tggtgctcgg ctcccatatg ctggaagtct gcccgtcgat
    16801 tgccgtagaa gagaaaccga tcctcgacgt tcagcatctc ggtattggtg gtaaggacga
    16861 tcctgcccga ctgatcttca atacccaaac cggtccagcg attgtcgcca gcctgattga
    16921 tctcggcgat cgttaccgtc tgctggttaa ctgtatcgac acggtgaaaa caccgcactc
    16981 cctgccgaaa ctgccggtgg cgaatgcact gtggaaagcg caaccggatc tgccaactgc
    17041 ttccgaagcg tggatcctcg ctggtggcgc gcaccatacc gtcttcagcc atgcgctgaa
    17101 cctcaacgat atgcgccagt tcgccgagat gcacgacatt gaaatcacgg tgattgataa
    17161 cgacacccgc ctgccagcgt ttaaagacgc actgcgctgg aacgaagtgt attacggatt
    17221 tcgtcgctaa gtagccacat caggtatgta acgcctgatg cgacgctgac tcgtcttatc
    17281 tggcctacac gctgcgattt tgtaggccgg ataagcaaag cgcatccggc attcaacgcc
    17341 tgatgcgacg ctggcgcgtc ttatcaggcc tacgcgctgc gattttgtag accggataag
    17401 cgaagcgcat ccggcacgaa ggagtcaaca tgttagaaga tctcaaacgc caggtattag
    17461 aagccaacct ggcgctgcca aaacacaacc tggtcacgct cacatggggc aacgtcagcg
    17521 acgttgatcg cgagcgcggc gtctttgtga tcaaaccttc cggcgtcgat tacagcgtca
    17581 tgaccgctga cgatatggtc gtggttagca tcgaaaccgg tgaagtggtt gaaggtacga
    17641 aaaagccctc ctccgacacg ccaactcacc ggctgctcta tcaggcattc ccctccattg
    17701 gcggcattgt gcatacgcac tcgcgccacg ccaccatctg ggcgcaggcg ggtcagtcga
    17761 ttccagcaac cggcaccacc cacgccgact atttctacgg caccattccc tgcacccgca
    17821 aaatgaccga cgcagaaatc aacggcgaat atgagtggga aaccggtaac gtcatcgtag
    17881 aaacctttga aaaacagggt atcgatgcag cgcaaatgcc cggcgttctg gtccattccc
    17941 acggcccgtt tgcatggggc aaaaatgccg aagatgcggt gcataacgcc atcgtgctgg
    18001 aagaggtcgc ttatatgggg atattctgcc gtcagttagc gccgcagtta ccggatatgc
    18061 agcaaacgct gctggataaa cactatctgc gtaagcatgg cgcgaaggca tattacgggc
    18121 agtaatgact gtataaaacc acagccaatc aaacgaaacc aggctatact caagcctggt
    18181 tttttgatgg attttcagcg tggcgcaggc aggttttatc ttaacccgac actggcggga
    18241 caccccgcaa gggacagaag tctccttctg gctggcgacg gacaacgggc cgttgcaggt
    18301 tacgcttgca ccgcaagagt ccgtggcgtt tattcacgcc gatcaggttc cccgcgctca
    18361 gcatattttg cagggtgaac aaggctttcg tctgacaccg ctggcgttaa aggattttca
    18421 ccgccagccg gtgtatggcc tttactgtcg cgcccatcgc caattgatga attacgaaaa
    18481 gcgcctgcgt gaaggtggcg ttaccgtcta cgaggccgat gtgcgcccgc cagaacgcta
    18541 tctgatggag cggtttatca cctcaccggt gtgggtcgaa ggtgatatgc acaatggcac
    18601 tatcgttaat gcccgtctga aaccgcatcc cgactatcgt ccgccgctca agtgggtttc
    18661 tatagatatt gaaaccaccc gccacggtga gctgtactgc atcggcctgg aaggctgcgg
    18721 gcagcgcatc gtttatatgc tggggccgga gaatggcgac gcctcctcgc ttgatttcga
    18781 actggaatac gtcgccagcc gcccgctgct gctggaaaaa ctcaacgcct ggtttgcaaa
    18841 ccacgatccc gatgtgatca tcggctggaa cgtggtgcag ttcgatctgc gaatgctgca
    18901 aaaacatgcc gagcgttacc gtcttccgct gcgtcttggg cgcgataata gcgagctgga
    18961 gtggcgcgag cacggcttta aaaacggcgt cttttttgcc caggctaaag gtcggctaat
    19021 tatcgacggt atcgaggcgc tgaaatccgc gttctggaat ttctcttcat tctcgctgga
    19081 aactgtcgct caggagctat taggcgaagg aaaatctatc gataacccgt gggttcgaat
    19141 ggacgaaatt gaccgccgtt tcgccgaaga taaacctgcg ctggcaactt ataacctgaa
    19201 agattgcgag ctggtgacgc agatcttcca caaaactgaa atcatgccat ttttactcga
    19261 acgagcaacg gtgaacggcc tgccggtgga tcggcacggc ggttcggtgg cagcgtttgg
    19321 tcatctctat tttccgcgaa tgcatcgcgc tggttatgtc gcgcctaatc tcggcgaagt
    19381 gccgccgcac gccagccctg gcggctacgt gatggattca cggccagggc tttatgattc
    19441 agtgctggtg ctggactata aaagcctgta cccgtcgatc atccgcacct ttctgattga
    19501 tcccgtcggg ctggttgaag gcatggcgca acctgatcca gagcacagta ccgaaggttt
    19561 tctcgatgcc tggttctcac gggagaaaca ttgcctgccg gagattgtga ctaacatctg
    19621 gcacgggcgc gatgaagcca aacgtcaggg taataagccg ctttcgcagg cgctgaagat
    19681 cattatgaat gccttttatg gcgtgctcgg caccaccgcc tgccgcttct tcgatccgcg
    19741 cctggcatcg tcgatcacca tgcgtggtca tcagatcatg cggcagacca aagcattgat
    19801 tgaagcgcag ggctacgacg ttatctacgg agataccgac tcgacttttg tctggctaaa
    19861 aggtgcacat tcggaagaag aggcggcgaa aatcggtcgt gcactggtgc agcacgttaa
    19921 cgcctggtgg gcggaaacgc tgcaaaaaca acggctgacc agcgcattag aactggagta
    19981 tgaaactcat ttctgccgtt ttcttatgcc aaccattcgc ggagctgata ccggcagtaa
    20041 aaagcgctac gccgggctga ttcaggaggg cgacaagcag cggatggtgt ttaaagggct
    20101 ggaaaccgtg cgcaccgact ggacgccgct ggcccagcag tttcagcagg agctgtacct
    20161 gcgtatcttc cgcaacgagc catatcagga gtacgtccgc caaaccatcg acaaactgat
    20221 ggcgggtgaa ctggatgcgc ggctggttta ccgtaaacgc cttcgccgtc cgctgagcga
    20281 atatcagcgt aatgttccgc ctcatgtacg cgccgctcgc cttgccgatg aagaaaatca
    20341 aaagcgcggt cgccccttgc aatatcagaa tcgcggcacc attaagtacg tatggaccac
    20401 caacggcccg gagccgctgg actaccaacg ttcaccactg gattacgaac actatctgac
    20461 ccgccagcta caacccgtgg cggagggaat actccctttt attgaggata attttgctac
    20521 acttatgacc gggcaacttg ggctattttg agcaaaaaaa agagttcgcc agataccatt
    20581 ttgatgcgtg acgaatgctt tgccatccag taccatagcg ccctttccat tcctggacct
    20641 gaataacacc actacctcat aagcacggta gcgggtggtt attgcctgca attaaagata
    20701 tagagccgaa cacatatgcc ttttacactt ggtcaacgct ggatcagcga tacagaaagc
    20761 gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca
    20821 tctactggtg aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc
    20881 aaccctggtg ataccattac cagccatgac ggctggcaga tgcaagtcga agaagtaaaa
    20941 gaagaaaatg gcttgctgac ctatatcggt actcgcctgg atactgaaga gtccggcgta
    21001 gccctgcgtg aagttttcct tgatagcaaa ctggtgttca gcaaaccgca ggaccgtctg
    21061 tttgccgggc agattgaccg tatggaccgc tttgcgctgc gttatcgcgc gcgtaagtat
    21121 tccagcgaac agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac cagcctgatc
    21181 ccgcatcagc tcaacatcgc tcatgatgtt ggtcgccgcc acgcgccgcg cgtcctgctg
    21241 gctgacgaag tgggtttagg gaaaaccatt gaagccggga tgatcctgca tcagcaactg
    21301 ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca gcatcagtgg
    21361 ctggtagaaa tgctgcgccg tttcaacctg cgctttgctc tgtttgatga tgagcgttat
    21421 gccgaagctc agcacgatgc ttacaacccg tttgacaccg aacagctggt gatttgctcg
    21481 ctggattttg cccgtcgtag caaacagcgc ctggaacatc tctgtgaagc cgaatgggac
    21541 ctgctggtgg tcgatgaagc gcatcacctg gtgtggagcg aagatgcgcc aagccgtgaa
    21601 tatcaggcca ttgaacaact ggcagagcac gtgccgggcg ttctgctgct gaccgcgacc
    21661 ccggaacagc tggggatgga aagccacttc gcccgtctgc gtctgctgga cccgaaccgt
    21721 ttccacgatt ttgcgcagtt cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc
    21781 gttgccatgc tgctggcagg taacaaactg agcaatgacg aactgaacat gctcggcgag
    21841 atgatcggcg agcaggatat cgagccgctg ttgcaggcag caaacagcga cagcgaagat
    21901 gcccagagcg cccgtcagga gctggtttcg atgctgatgg atcgccacgg caccagccgc
    21961 gtgctgttcc gtaacacgcg taacggtgtg aaaggattcc cgaaacgcga gctgcacacc
    22021 attaagctgc cgctaccgac gcagtatcag acggctatta aagtctccgg cattatgggc
    22081 gcacgtaaaa gtgcggaaga tcgtgctcgc gatatgctct acccggagcg tatttatcag
    22141 gaatttgaag gtgataacgc cacctggtgg aacttcgatc cgcgcgttga gtggctgatg
    22201 ggctacctga ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa agctgccact
    22261 gcgctgcaac tggagcaggt actgcgcgaa cgtgaaggta ttcgcgctgc ggtgttccac
    22321 gaaggtatgt cgattatcga acgtgaccgc gctgccgcct ggtttgccga agaagacacc
    22381 ggcgcacagg tactgctgtg ctcagaaatc ggttctgaag gacgtaactt tcagttcgcc
    22441 agccacatgg tgatgtttga cctgccattc aacccggatc tactggagca gcgtattggt
    22501 cgtctggatc gtatcggcca ggcgcacgat attcagatcc atgtgcctta tctggagaaa
    22561 accgctcagt cggtgctggt gcgctggtat cacgaaggtc tggatgcatt tgagcacacc
    22621 tgcccgaccg gacgcactat ttacgatagc gtatacaacg atctgattaa ctatctggct
    22681 tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa gctgccgcga gcaacatgaa
    22741 gcgctgaaag cacagctgga acagggtcgt gaccgcctgc tggaaatcca ctccaacggt
    22801 ggcgaaaaag cccaggcact ggcagaaagc attgaagagc aggatgacga taccaacctg
    22861 atcgccttcg ccatgaacct gttcgatatt atcggtatca atcaggacga tcgcggcgac
    22921 aacatgatcg tgctgacgcc gtccgatcat atgctggtgc cggacttccc tggcctgtcg
    22981 gaagatggca tcaccatcac ctttgatcgt gaagtggcgc tggcgcgtga agatgcacag
    23041 tttattacct gggagcatcc gctgatccgc aacggtctgg atctgatcct ttctggcgat
    23101 accggtagca gcacgatttc actgttaaaa aacaaagcgt tgccggtagg tacgctgttg
    23161 gtggaactga tttatgtggt tgaagcccag gctccgaagc agttgcagct caaccgcttc
    23221 ctgccaccga cgccggtacg tatgctgctg gataaaaacg gcaacaacct ggcggcgcag
    23281 gtagagtttg aaacctttaa ccgccagctt aacgcggtta accgtcacac cggcagcaaa
    23341 ctggttaaca ccgtgcagca ggatgttcac gctatccttc aactgggtga agcgcagatc
    23401 gagaaatctg cccgtgcatt gattgatgca gcgcgtaacg aagccgacga aaaactgtct
    23461 gccgagctgt ctcgtctgga agctctgcgt gcagtgaacc cgaacattcg tgacgacgaa
    23521 ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca ggcaggttgg
    23581 cgtctggatg ccctgcgttt gatcgttgta acgcatcagt aacggagccg aaaatgggga
    23641 tggaaaacta caatccaccg caggaaccct ggttggttat cctgtatcag gatgaccata
    23701 ttatggtggt caacaagccg agcggtttgt tgtcagtgcc gggtcgtctg gaagagcaca
    23761 aagacagcgt gatgacgcgc attcagcgtg attatccgca ggcagaatcg gtgcatcgtc
    23821 tggatatggc taccagcggc gtgattgtag tggcgctgac caaagccgcg gagcgggagt
    23881 taaaacgcca gttccgcgag cgcgagccga aaaagcagta tgtggcccgc gtctgggggc
    23941 atccatcccc cgcagaaggt ctggtggatc tgccgctgat ttgcgactgg ccaaaccgcc
    24001 cgaaacagaa agtctgttac gaaacgggta aacctgcgca gacggaatat gaagtggtgg
    24061 agtatgcggc ggataacacg gcaagagcgg tgttaaaacc gattaccggg cgttcgcatc
    24121 aattgcgtgt gcatatgctg gcgctggggc atccgattct cggcgatcgt ttttatgcat
    24181 caccagaagc gagagcgatg gcaccgcgtt tgttgctaca tgcagagatg ttgacgatta
    24241 cccatccggc gtatggcaat agtatgacgt ttaaagcgcc agcggatttt taatcgtcac
    24301 gctggcgcag ttcggtaggc ggataaggcg ttcacgccgc atccggcaat tgtgcgccga
    24361 ttgcctgatg cgacgcgggc cgcgtcttat caggcctaca gtttaaaact tatttaaacc
    24421 ctttctgctg ctttatcagc tcatatgcct gctgaatttc ctgcgctttc tgcttcgcca
    24481 tctccatcat ctcaggcggc aaacctttcg ccaccagctt atcgggatgg tgttcactca
    24541 tcagcttacg gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc acgcccagca
    24601 cattacaggc atcttccagc gtcgggccac gctgcgcttg ctgccagtta ccaccgccag
    24661 attgctgctg ataaccgccg ccaaactgtg caccgccctg catcatgcgc aaaaactggt
    24721 caaactgggc gcgggagatc cctaattctt cagcaatgac atacagcacc gcccgttcat
    24781 tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg gatctccaga aacatacgaa
    24841 ttaagtcaaa acgaccaaag cagacactgc gaaactggcg catcttttcg cgcagcgggt
    24901 aattatctga ttttcccacc cggaacgcgt tttgcgccgc agtacgggaa gcgccatgaa
    24961 ggttcattcg gtccatcaac tggctggcga tatgaatatc agcctccgtg acgcgacctt
    25021 tggatttggt taaatgcccc atcacttcaa aagtggtggc aaaaaacagc gcctgacgct
    25081 cacgctggtt ggcgaaccac gccattttac ggctacgggc tttatcaaac atatggccaa
    25141 ttaacaggcc taacactacg ccccaaaagc cgccgcccat cagtaaggcc acggccacgc
    25201 caatgatttt tccccaatac tgcatatatt ccccaaatcg acacacggat atcagggcta
    25261 tctcccacaa tataaaggtg cttttaccgt tttccggctg cggtcaattg tgggacatcg
    25321 cctataattt gcattatcat acctgtcatt caatgccgtg cctaacacca ctgacgctat
    25381 tcggacagga ttaacactag cgtagagatg acgagtacgt tagtctctga ccgtttgtca
    25441 cgcgcaacgt taccgatgat ggaacaataa aatcaacgta tgaaaaaacg tatccccact
    25501 ctcctggcca ccatgattgc caccgccctt tatagtcaac agggactggc agccgacctc
    25561 gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc
    25621 aatgacttac ccgtgactat caatgctgac cacgcgaaag gggactaccc ggatgacgcc
    25681 gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg
    25741 cagctccatc aaaaagaggc accaggacaa ccggagccgg tacgtaccgt tgatgcgctc
    25801 ggtaatgtcc attacgacga taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat
    25861 ctgaacacca aagataccta cgtctgggaa ggtgattacc agatggtggg tcgccagggt
    25921 cgcggtaaag cggacctgat gaaacaacgt ggcgaaaacc gctataccat tctggataac
    25981 ggtagcttta cctcctgtct gccgggttct gacacctgga gcgtggtagg tagcgaaatt
    26041 attcatgacc gcgaagaaca agttgcggag atctggaacg cccgctttaa ggtgggtccg
    26101 gtaccgatct tttatagccc ctatttgcag ttgccggtgg gtgacaaacg tcgctctggt
    26161 ttcttgatcc cgaacgccaa gtacaccacc accaactact ttgagttcta cctgccatat
    26221 tactggaaca tcgcgccaaa tatggatgcc accatcacgc cgcattatat gcatcgtcgt
    26281 ggcaacatca tgtgggagaa cgaattccgc tacctctccc aggcgggcgc tggcttgatg
    26341 gaactggact atctgccttc agataaagtc tatgaagatg aacacccgaa cgatgacagt
    26401 tcacgtcgtt ggttattcta ctggaaccac tccggggtca tggatcaggt gtggcgtttc
    26461 aacgtcgact acaccaaggt cagcgatcct agctacttca atgatttcga taacaagtac
    26521 ggttccagta ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc ggtgcaaaac
    26581 ttcaatgcca ccgtttcaac caagcagttc caggttttca gcgaacagaa caccagtagc
    26641 tactcggcag agccgcagtt agacgttaat tactaccaga atgatgttgg tccgtttgat
    26701 acgcgtattt acggccaggc agtgcacttt gttaacacca gagacgacat gcctgaagca
    26761 acccgtgttc acctggaacc gaccatcaat ttgccgctct ctaataactg gggcagcatc
    26821 aataccgaag cgaagtttct ggcaacccat tatcagcaaa ccaatcttga ctggtataac
    26881 tccagaaaca cgaccaagct ggacgaatcc gttaaccgcg taatgccgca attcaaagtt
    26941 gacggcaaaa tggtctttga acgcgatatg gaaatgctgg ctccgggtta tacccaaacg
    27001 ctggaaccgc gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga catctataac
    27061 tacgactcgt ctctgctgca atctgactac tctggcctgt tccgggaccg gacttacggc
    27121 ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc tcgcatatat
    27181 gatgatgctg ccgttgaacg ttttaatatt tccgttggtc aaatctacta tttcacggag
    27241 tctcgcactg gcgatgacaa cataacatgg gagaatgacg acaaaacggg ctcactggtg
    27301 tgggcaggcg acacctactg gcgtatctcc gagcgttggg gattgcgtgg cgggattcag
    27361 tacgatacac gtctggataa cgtagcgacc agtaactcca gcattgaata ccgtcgggat
    27421 gaagaccgtc tggtacagct gaattaccat tacgccagcc cggaatatat tcaggctacg
    27481 ctgcctaagt actattccac tgctgagcaa tataagaatg gtatttcgca ggtaggtgct
    27541 gtcgccagca ggccaattgc cgatcgttgg tccattgttg gggcctacta ctacgacacc
    27601 aatgcgaaca agcaagccga ctctatgtta ggtgtgcaat acagctcctg ctgctatgca
    27661 attcgcgtcg gttacgagcg gaagctgaac ggttgggata acgataaaca acatgcggta
    27721 tatgacaacg caatcggctt taacatcgaa cttcgcggcc tgagctccaa ctacggtctg
    27781 ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt
    27841 gatttaccac gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga agaactggaa
    27901 aacgctgctt ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc cccaggtagt
    27961 cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt
    28021 aatgcagtcg gtaaaactga acgctgcgca ggcaaggcag cagcttcctg atgacgcgac
    28081 gctgcgccac caaatcatgg aacgtttgat catggatcaa atcatcctgc agatggggca
    28141 gaaaatggga gtgaaaatct ccgatgagca gctggatcag gcgattgcta acatcgcgaa
    28201 acagaacaac atgacgctgg atcagatgcg cagccgtctg gcttacgatg gtctgaacta
    28261 caacacttat cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc gtaacaacga
    28321 ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc agcaggtggg
    28381 taaccaaaac gacgccagca ctgagctgaa cctgagccac atcctgatcc cgctgccgga
    28441 aaacccgacc tctgatcagg tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca
    28501 ggcgcgtaac ggcgctgatt tcggtaagct ggcgattgct cattctgccg accagcaggc
    28561 gctgaacggc ggccagatgg ggtggggccg tattcaggag ttgccgggga tcttcgccca
    28621 ggcattaagc accgcgaaga aaggcgacat tgttggcccg attcgttccg gcgttggctt
    28681 ccatattctg aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt
    28741 tcatgctcgc catattctgc tgaaaccgtc gccgatcatg actgacgaac aggcccgcgt
    28801 gaaactggag caaattgctg ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc
    28861 gaaagagttc tcgcaggatc caggttctgc taaccagggc ggtgatctcg gctgggctac
    28921 accagatatt ttcgatccag ccttccgtga cgccctgacc cgcctgaaca aaggtcaaat
    28981 gagtgcaccg gttcactctt cattcggctg gcatttaatc gaactgctgg atacccgtaa
    29041 tgtcgataaa accgacgctg cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa
    29101 gctctcggaa gaagcagcaa gctggatgca ggaacaacgt gccagcgcct acgttaaaat
    29161 cctgagcaac taatggttaa aacccaacgt gttgtgatca ctcccggcga gcccgccggg
    29221 attggcccgg acttagttgt ccagcttgca cagcgtgagt ggccggtcga actggttgtt
    29281 tgtgccgatg ccactctcct taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc
    29341 cgcccttatt cccccaactc ccctgcacaa ccgcaaacta cgggcacatt aacgctactt
    29401 cctgtcgcgc tacgtgaatc tgtcactgcg gggcagttag cgattgaaaa tgggcattac
    29461 gtggtggaga cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt tgctgcgctg
    29521 atcacaggcc ccgtgcataa aggcgtcatt aacgacgcaa gcattccgtt taccggtcat
    29581 accgagtttt tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa
    29641 gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc agatgctatc
    29701 acccctgcac ttttgcacga agtgattgct attttgcatc acgatttgcg tacaaaattt
    29761 ggtattaccg aaccgcgcat tctggtctgc gggctgaatc cgcacgcggg cgaaggcggt
    29821 catatgggta cggaagagat agacaccatt attccggtgc tcgacgagct gcgggcgcag
    29881 gggatgaaac tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa atatctcgat
    29941 aacgccgacg ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct aaaataccag
    30001 ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct ttattcgcac atcagtggac
    30061 cacggcaccg cgcttgaact ggcgggacgt ggcgaagccg atgtcggcag ttttattacg
    30121 gcgcttaatc tcgccatcaa aatgattgtt aacacccaat gaataatcga gtccaccagg
    30181 gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca
    30241 gcattgtttc tgccattaac ccgcaaaagg gccaggcaat ggtcgaaatc ggccccggtc
    30301 ttgcggcatt gaccgagccg gtcggcgaac gtctggacca gctgacggtc atcgaacttg
    30361 accgcgatct ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa ctgacgattt
    30421 atcagcagga tgcgatgacc tttaactttg gcgaactggc cgagaaaatg ggtcagccgc
    30481 tgcgtgtttt cggcaacctg ccttataaca tctccacgcc gttgatgttc catctgttta
    30541 gctatactga tgccattgcc gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc
    30601 tggttgcagg accgaacagc aaagcgtatg gtcgattaag cgtcatggcg caatactatt
    30661 gcaacgtgat cccggtactg gaagttccgc cgtcagcctt tacaccacca cccaaagtgg
    30721 attccgccgt cgtgcgcctg gtccctcatg caacgatgcc tcacccggtt aaagatgttc
    30781 gcgtgttgag ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc attcgtaaca
    30841 gcctcggcaa cctgtttagc gtcgaggtgt taaccggaat ggggatcgac ccggcgatgc
    30901 gagcggaaaa tatctctgtc gcgcaatatt gccagatggc gaactatctg gcggagaacg
    30961 cgcctttgca ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag
    31021 cgtctacatt gaggcacaat cttcacctga taatgaacgt tacgtttttg cttataccgt
    31081 aaccatacgc aatctggggc gagcgccagt gcagttgttg gggcgttact ggctgatcac
    31141 caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg tccagccact
    31201 tatcgcgcct ggcgaagagt accagtacac cagcggtgca atcattgaaa ccccgctggg
    31261 caccatgcag ggtcactacg aaatgatcga tgaaaatggc gtccctttca gcatcgacat
    31321 tcccgtattc cgactcgccg ttcccacact cattcattaa aagaatatgg cgacatacct
    31381 tattggcgac gttcatggtt gttacgatga actgatcgca ttgctgcata aagtagaatt
    31441 tacccccggg aaagataccc tctggctgac gggcgatctg gtcgcgcgcg gcccgggttc
    31501 gctggatgtt ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa
    31561 tcacgatctg catctgctgg cggtatttgc cgggatcagc cgcaataaac cgaaagatcg
    31621 cctgacaccg ctgctggaag cgccggatgc cgacgagctg cttaactggc tgcgtcgcca
    31681 gcctctgctg caaatcgacg aagagaaaaa gctggtgatg gcccacgcag ggatcacgcc
    31741 gcagtgggat ctgcagaccg ccaaagagtg cgcgcgcgat gtagaagcgg tgctgtcgag
    31801 tgactcctat cccttctttc ttgacgctat gtacggcgat atgccaaata actggtcacc
    31861 ggaattgcgg gggctgggaa gactgcgttt tatcaccaac gcctttaccc gtatgcgttt
    31921 ttgcttcccg aacggtcaac tggatatgta cagcaaagaa tcgccggaag aagcccctgc
    31981 cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct gaagaataca gcatcgcctt
    32041 tggtcactgg gcatcgctgg aaggcaaagg tacgccggaa ggtatttacg cgctggatac
    32101 cggctgctgc tggggtgggt cattaacctg cctgcgctgg gaagataaac agtattttgt
    32161 ccagccgtcg aaccggcata aggatttggg cgaagcggcg gcgtcttaaa cacagcctga
    32221 tacaggaagg ccggataaga cgcgaccggc gtcgcatccg gcactagccg taaattctat
    32281 acaaacttac cgccgctcca gaatctcaaa gcaatagctg tgagagttct gcgcatcggc
    32341 gtcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg ggaaatgggt
    32401 gtcgccttcc acttctgcgt cgatatgcgt cagatacagt ttttgcgctt ttggcaggaa
    32461 ctgctcataa acgcgaccgc cgccaatcac catgatttct ggtacgtcac cacacgccgc
    32521 aatggcttca tccaccgatt tcacccacgt tacgcgatcg tccgtacccg gttgactgct
    32581 gaggataata tttttgcgtc ctggcaacgg acgaccgatt gattcccaag tatggcggcc
    32641 cataatcacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt
    32701 ccacggcatg gcgttttcca tgccgataac gcgatctacc gctaacgccg caatcagact
    32761 gatcattgag atttcccgat aaaaaaattg tcgccactat acgtaaagcg taaaccgtcg
    32821 tcgactggtg cgaggatgat gttgaggaaa attttatatt ctgctggcga gtccacgctc
    32881 tccccctgga ctcgccgcat tacaatgaaa caaaaacaaa cagttagctg taaagtgtga
    32941 tttacgtcac tctttattaa gatgagggtt tcgtttccgg ttcatccgcc atgttgccgg
    33001 tatgtttacc ttcttctgtt ccctgccagc catgtcgttg aattaatgac agatgttcgc
    33061 ggtcctcggt gatgatctca cttaacatcg cgctggtgcg tttatagacc gccgcgcggg
    33121 ctttggtgtc attctcaacc attgccatct cttccaccat ctgaatatta aagcggcgga
    33181 acacatcggc acgttctcgc gcttcatacg gccccagacc taaactttcc agcgccagac
    33241 gcccggtttt cagcgcacct tcaaaggttt cacgctccgg cttttcaacg cctgcctgac
    33301 gcaaacgaat gtagtgatcg acatcgcggg cgcgggcaat aatctgcaaa tgcgggaaat
    33361 gttctttcac catctccgtc agttgcaggt tggtttgcgg atcgtcaata gcgttaatca
    33421 gcacttccgc tttcgccgct ccggcagatt ccagtaaatc catccgcgtg gcatcgccat
    33481 aaaacacttt cataccaaat ttacgcagcg tttcgatatg gtccggatcg tgatcgagca
    33541 ccaccatttt caccccgctg gagagcagta aacgtccggt aatctgtcca aaacgaccga
    33601 atccggcgat aatcacgcgc ggctgttctt catcgatctc atcggcttca cgcgcctcct
    33661 ccgtagaaga ttgctcaagg cgattgagga tcaccagcag aatcggcgtt gctgccatcg
    33721 acagcgccac cgccagggtc agcgatttcg cccactccgg ctccagcaca ttcgccatct
    33781 gcgccgcacc aaataccaca aaggcaaact cactgccctg ccctaacaac accgcaaacc
    33841 agcgacgctg tttatttggc acctgcaacg gtcgggcaat cagccacagc atggcgattt
    33901 tgatgatgag gaaaccgagc agcaaaatga caatgcgcaa tgggttttca atcagcgtgc
    33961 caaagtctat cgacatgcca acaccgatga aaaacagccc caacagcaaa cctttaaacg
    34021 gttcgatatc gctttccaac gcatgacggt attccgagct tgccagcagt acgcccgcca
    34081 gaaacgcgcc catcgccatc gacaagccga cctcttccag cagcaaacca aagccaaaca
    34141 caaggaataa cgccacggca ctaaacactt cccgcaagcc agagcgggca acaaaacgca
    34201 gcgccggacg cgtaacatag cgccccagca ataccaccag taccagcgca cccgccactt
    34261 ttaacgccga gagagcaaat gcgcccatcg tcgtcgaggc actgctcgcc gccagtagcg
    34321 ggatcatcgc caccagcggg attgccgcga tatcctggaa cagcagcact gcaaaggcac
    34381 tgcggcccat ttgcgtcacc atcagattgc gttcattcat cgcctgcatg gcaatcgccg
    34441 ttgatgagag cgccagcgtc ataccgatca attccgcgac ctgccagcgc agcccaagta
    34501 acatacagaa cagccccagc aggccaccgc aaatcaccat ctgcaatgcg ccaccgccga
    34561 acactgccgc acgcagcttc cacagccttt gtggatcgag ttcgaggccg ataataaaca
    34621 gcatcagcac caccccaatc tcggcaaagt gcagaataga ttcggcatcg gtcaccagtc
    34681 gcagccccca cgggccaata atgcagccgg cgatcaggta gccaagtacc gatcccagac
    34741 caagacgtac cgcaatgggt acaatcagcg ccgccgatcc gaggtagatc agcgcctgaa
    34801 ccagcgtatg actatccatg atgggcctcc tgccattcca gcagacgttg cttatagtgg
    34861 cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat ggcaaacggt
    34921 ggcagccagt tcagcccgca gtagattgcc gtcgcctgta gcggctgcga cagcacatca
    34981 aagcccggat gcgcaccaat ttcaaaatgg ctttccccgc cgccggtcgt caccgcccac
    35041 agcaaatgtt tgccatgcag cgccgtgccg ccgtgaccgt aagcccagcc gtgcgacaaa
    35101 actttatcga tccaaagttt aagaagcgga ggaatgctgt accactgcat cgggtgctgc
    35161 cagacgatca gatcggcgcg agagagcgcc tcctgctcgg cggcaatatc gatattgaag
    35221 tcaggataga gttgataaag agagcgaatt tcgacgcctt ccagcgtcct tgcctgttca
    35281 agcatccgtt tattcgcatg ggaatgatgc ggatacggat gcgcataaat gatgaggatc
    35341 atagttatta gcccgcgtgt ttacgccttt gttttcccac aaagtgtagt catatattca
    35401 cgcggcttat agtcaataat attgattcag aaaagagaga aatctgaaca aataatccca
    35461 ttctctgccc gcggatcgtc atattgctgg tctgcgccag tgacattcct cgagtctccg
    35521 gggcaaacgc tacggaaatc aacaagccaa acagcgagat acccgccccc attagcatcg
    35581 tgttactgat accgtaatta ttgataaata tcggcagtgc ccaggtcgaa acaatggtgc
    35641 caatacgact taaggacata atcacgccca cggcagaggc gcggatatcc gtcgggaaga
    35701 gttcattagg atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg
    35761 caaaggccat cactaccagc cagatcccca tatccgggat tagccccaaa accgccagcg
    35821 ccagcgtcat catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttagcc
    35881 acagcatcgg cggaatacag ccgagcataa agaacaggct aatcaccaca ttccctagtg
    35941 ccgcgttttt gccaaccccc aatcccaacg aaccaacgat ttgcgggcca aaggtgtaaa
    36001 tggcgaacat tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa
    36061 aatggcggcg attaaacaga tcgcgaaaac gggtttgctg cggctgctct tcatcgaaag
    36121 ccaccggttc gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc
    36181 gtaataacca gcggggagat tctggcagtt cgaatcgacc aatcaaaatc aacaaacaag
    36241 ggatcgccgc gctacccagc atccagcgcc aaccaccttc cacatcataa agccagtagc
    36301 cgaccagatc ggcgcaggtc gcgccgacat accacatcgc ggcaataaag ctgatggaaa
    36361 acgcccgctg acgggtactg gagaactcgg tgatcatcga agtggcgatg ggataatctg
    36421 caccgatgac aatgccgatg agtacccgca tcaccaacag ttcgacgggg gatgaaacac
    36481 acatcgtcgc caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc
    36541 gtccgacttt atcggagata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg
    36601 ttcccgcgcc cagcaagcca atccagtcag cgtccagttt cagcgccgga gtcagttgct
    36661 ccagcgccac gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc
    36721 acagcaaaat gcggcggtga atagaggaga atttgagatc gtcaaagttt ctggacggtt
    36781 gcatgggtta ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa
    36841 catcagccgt aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc
    36901 gccgacccca gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag
    36961 cgcacactgc cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc
    37021 tccagcacct gtttatcagc atcggccttc acaacaatgt gcggatgctc ttcatcgaca
    37081 ttgaatttat tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca
    37141 ctcctttcat gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga
    37201 ttttcttgcg catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc
    37261 ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaac gccggtagtt
    37321 tctggtacat gcgcatatcg cgcagcgggc tgctctccag atgctgacga tattccgcga
    37381 gtttttgctt actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcagctt
    37441 ccccggcggc aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc
    37501 cggcggcatc accggcaatc aatacgccgt caccgaccag ctccggcagc atgttgatgc
    37561 ctgcttccgg cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg
    37621 caacggccgg atgctgtttg aaatcttcca gcatttgcgg caccgatttt ttcgcgtcat
    37681 gcagatgatg cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga
    37741 agccgccgcc catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat
    37801 taccctgcaa ctgaaaacgg tcttcgatta ccgacttcgg tgactcgatc agttccttca
    37861 cgccaaccgc cacatccgtc ggtttaacgc gttttgccat ccccaacttt tcggcaagga
    37921 tggagttcac cccatcagca aggagcaccg ttttcgcttc aatcacatcg ccatcggctt
    37981 ctacaccgac gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta
    38041 actgcgcgcc cgcttcttcg gcctgctcca tcaaccaggc atcaaattta ctgcgcaaaa
    38101 cggagtaaga acgctgggat agcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg
    38161 ctgacttttc cgtcataaac gcgagttttt catgggtgat caggcgttct acggggcgga
    38221 atcggcgaaa cctggaataa tgtgttccag actgtgggca tagagacgcc cgccagtaac
    38281 gttcttggca cccgcggaat tgccacgctc gataactaac acttgcgccc cttcgcgggc
    38341 gagcaccagt gcggcaaccg aaccggcaag ccctgcaccg acgatgatgg cgtcaaagat
    38401 atcttcggac ataagagctc cctgtcagcg gcagagccgt aatgcagccc tgccagagtg
    38461 gatcaacgcg ctaaagctgc ggtcagtgcc ggaaggatct tcacggcatc gccaacaatg
    38521 ccgtaatccg cgtactggaa gatcggcgca tttttatctt tattgatggc gaaaatggtt
    38581 tgcgacgcgt tagcgccaac catgtgctgg atctgcccgg agatcctcac cgccaggtac
    38641 agttcaggtt tcagcatcag gttggagata ccgacatagc gttcgtgctc catccatttt
    38701 tcgttttctg ccaccggacg agaacaggcc aactccgcac ctatcgcctt gcaaagctgt
    38761 tctgccagtg caatgttctc tttgctgcca atgccgcgac cgacgctgac caccagacgg
    38821 gctttgtcga gatcgacgct gttgctctgg cgcgtctggg ttgccgtgcg ggtaatcgcc
    38881 acagccggag cctgccactc cacggtgtgc gtttcgccag tgcgtgacgc gtctggctga
    38941 gccgcatcga acgtgccgct gctgatggtc agtaccgcat acggcgtggc aatgcgttct
    39001 tcgccaatcg ccagaccgcc gtaaaccatg tgtttcactg ttgctttacc gtcctgtacg
    39061 ctgacggtgc tggcatcgtt agacaccgcc gctttaaggc gataacccag ttttgccgcc
    39121 agtaatttgc cgcgacgggt gtttggcaac agcaccagac cgtcagcgcc gtgctggcga
    39181 atagtgtcag ccatgacacc ggcgtaatct tcgatcatcc gatcgtccgg tttgccgttt
    39241 aatttccaga catgattagc gccgagctgg attgcctgtg cgccgtcggc atcattgagg
    39301 acaaaggcat tgatttgatt agctaaagcc tgcgcaccgt tcatcagttc cggcagacga
    39361 gagggggtat cgctgaatac ccagacttga gaaaacgtgt tcatagcatc ccctgtaatt
    39421 aaatgacttt gcgaagattt tcagcaaatg cggcgatctg ttcttcgccg tcgccttcaa
    39481 tcacgatgcg ctgacgctcg cgctgtttcg gcgcggcaac ctgttgttca gaccaggcgt
    39541 ccacggcgtt aaaaccaata tccgccgccg accatacctg gactggcttt ttcgccgcgc
    39601 cgagaatggc tttcatcgaa ggaatttgtg gggagttgat atcagtggaa acagcaacaa
    39661 ccgcaggcag cggaatgctt aaggtttcgg tttcatcttc cagttcgcgc tcaacggtga
    39721 gggtatctgc cgtcagggag ataattttgc tgacgccgtt aactgccgga atattgagga
    39781 tttcgcccac cagcagacca acctgctggg cataaaggtc ggaagaacca tcgccacaga
    39841 ggatcagatc aaagcctgct ttctgggcgg ctgcagccag tgcgctcgcc gtttgttgcg
    39901 gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag ttcatccggg ccgcgcgata
    39961 gcacatcttt acgccctttg gcgttggtca gggctttacc gcccacactt aaggctgtca
    40021 cctgcgcctc tgctgcctgt tgctttagct ggcaagccgc ttcaatagcg ttgagatcgt
    40081 attggcttat tttggcatcg gctttgctga agtctaatga accatcagca ttattgaccg
    40141 caatatcctg ttcatcaggc acgcacttat agcaagtaat aatcttcatt gcatctccag
    40201 aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat tacagatatc acgcccttta
    40261 atttggaaat aaaactttaa tcaccaatat tgaaaatgtc acacgcagat aaatccaact
    40321 ttcaatattg ttaagttcct caccaatatt gaaaacatgg cgtagcaaaa agaaattttc
    40381 aatattgttt tatagatcac caatattgaa agtcagatgc tattttttac ccacaaaaga
    40441 acataacaaa ctgattaata accaaaatta acgaaaaaca tcctctattc actcgaaaca
    40501 aaaatgtgac accaatcaca gaatacagct tattgaatac ccattatgag ttagccatta
    40561 acgcgtccac gaggttaata ataattatat taaatgttaa caaaaataaa acaaacggga
    40621 acgcaaaata aatattcgtt ttcacagtgg aattaactca tgaagaatga aaagagaaaa
    40681 acgggaatag aaccgaaggt tttctttccg ccattaataa tcgtcggcat actttgttgg
    40741 cttacagtca gagatctgga tgcggcgaat gtcgttatta atgctgtatt cagttacgtc
    40801 accaatgtat ggggatgggc atttgaatgg tatatggtgg tgatgctttt cggttggttc
    40861 tggctggtgt ttggcccgta tgccaaaaag cgtttaggta acgaaccgcc agaatttagc
    40921 accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg
    40981 ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga accgaactcg
    41041 acaggagcga aagagttggg gctggcttac agcttgttcc actggggacc tctgccgtgg
    41101 gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg caaaatggaa
    41161 gtgattcgcc ccagctcgac actggtgccg ctggtaggtg aaaaacacgc caaagggttg
    41221 ttcggtacca tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat gggtaccagt
    41281 ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat
    41341 accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc catttgcgtc
    41401 gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc
    41461 ctgatgctgg gttgggtgtt catcgtcagc ggtgccagct tcatcatgaa ctacttcacc
    41521 gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt tgttctatac cgatcccatc
    41581 gctaaaggcg gcttcccgca gggctggacc gtgttctact gggcatggtg ggtgatttac
    41641 gccatccaga tgagtatctt cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg
    41701 tgcttcggca tggtgatggg gctgacagca tccacctgga tcctgtggac cgtgctcggt
    41761 agtaacactc tgctgttgat ggataaaaac atcatcaaca ttccaaatct gatcgaacag
    41821 tacggtgtgg cgcgcgccat catcgaaacc tgggccgctc tgccactcag caccgccacc
    41881 atgtggggct tcttcatcct ctgctttatt gccaccgtta cgctggttaa cgcctgctct
    41941 tataccctgg cgatgtctac ttgccgcgaa gtacgcgatg gtgaagaacc accgctgctg
    42001 gtgcgtatcg gttggtcaat tctggttggc attatcggta ttgttctgct ggcgctcggc
    42061 ggcctgaaac cgattcaaac cgccattatc gccggaggat gcccgctgtt cttcgtcaac
    42121 attatggtga cgctctcctt tattaaagac gcgaaacaga actggaaaga ttaattaacc
    42181 cccaaaatat caagaggttg aaagatggat tttaatttaa atgatgagca ggaactgttt
    42241 gtcgccggta tccgcgaact gatggccagc gaaaactggg aggcctattt tgccgagtgc
    42301 gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat gggtatcgac
    42361 agtctgctga tccctgaaga gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc
    42421 gtgtggatgg agctgggacg tctgggggca ccaacctatg tactgtacca gctgccgggc
    42481 gggttcaata ccttcctgcg cgaaggcaca caagagcaga tcgacaagat tatggctttc
    42541 cgcggcaccg gtaagcagat gtggaactca gcgattaccg aaccgggcgc gggctccgac
    42601 gtgggtagcc tgaaaacgac ttatacccgt agaaatggta agatttatct taatggtagt
    42661 aagtgtttta ttaccagtag cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg
    42721 gcttctccgg acaaacctgt ctacaccgaa tggtttgttg atatgagcaa accgggcatc
    42781 aaagtgacca aacttgagaa gctcggtctg cgtatggata gctgctgtga aatcaccttt
    42841 gacgatgtgg aactggacga gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc
    42901 gtcaaagaag agttcgacca tgaacgtttc ctggtagccc tgaccaacta cggtacggcg
    42961 atgtgcgcct ttgaagatgc ggcgcgctac gccaaccagc gcgtgcagtt tggcgaggct
    43021 attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc
    43081 atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca acggcaccat cacctctggc
    43141 gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca
    43201 atgcaggtgc tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg cttctggcgt
    43261 gacctgcgtg tagaccgcgt ttccggagga tctgacgaaa tgcagatcct gacgctgggt
    43321 cgtgcggtgc tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac gctgacgcgt
    43381 cttatcaggc ctacgggagc acgaatgtaa gccggataag gcgtttacgc cgcatccggc
    43441 agtcatgcgc tacataaatt tttcaggagc taataatgga tcatctaccc atgccgaaat
    43501 tcggaccgct ggccggattg cgcgttgtct tctccggtat cgaaatcgcc ggaccgtttg
    43561 ccggacaaat gttcgcagaa tggggcgcgg aagttatttg gatcgagaac gtcgcctggg
    43621 ccgacaccat tcgcgttcaa ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc
    43681 tgtcgttaaa tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca
    43741 ccgatatctt catcgaagcc agtaaaggtc cggcctttgc ccgtcgtggc attaccgatg
    43801 aagtactgtg gcagcacaac ccgaaactgg ttatcgctca cctgtccggt tttggtcagt
    43861 acggcaccga ggagtacacc aatcttccgg cctataacac catcgcccag gccttcagtg
    43921 gttacctgat tcagaacggt gatgttgacc agccaatgcc tgccttcccg tataccgccg
    43981 attacttttc tggcctgacc gccaccacgg cggcgctggc agcactgcat aaagtgcgtg
    44041 aaaccggtaa aggcgaaagt atcgacatcg ccatgtatga agtgatgctg cgtatgggcc
    44101 agtacttcat gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta
    44161 aagatcccta ctacgccggt tgcggtctgt ataaatgtgc cgacggctac atcgtgatgg
    44221 aactggtggg cattacccaa attgaagagt gctttaaaga tattggcctc gcacatctac
    44281 tcggtacgcc ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa tgcccttacg
    44341 gcccactggt agaagaaaaa ctcgatgcct ggctggcggc acataccatc gcagaagtta
    44401 aagaacgctt tgccgaactg aatatcgcct gtgccaaagt actgaccgta ccggaactgg
    44461 aaagcaatcc acagtatgtt gcccgtgaat caatcactca gtggcaaacg atggatggtc
    44521 gcacctgcaa agggccgaac atcatgccga aattcaaaaa taaccccgga caaatctggc
    44581 gcggaatgcc ctcacatggc atggacacgg ctgccatttt gaaaaatatc ggctacagcg
    44641 aaaacgacat tcaggagttg gtcagcaaag gtctggccaa agttgaggac taaacgcgtg
    44701 gcgtcagtcg gcttgcctga ctagcgcccg cccaataaca aacgaacaaa aatggataga
    44761 ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa tgtgggacga tctggcggac
    44821 gtttacggtc ataaaacggc gctgatttgt gaatccagcg gcggagtcgt taaccggtat
    44881 agttatcttg agttaaatca ggagattaac cgcacggcaa acctgtttta tacgctgggg
    44941 attcgcaaag gcaacaaggt tgcactacat ctcgacaact gcccggaatt tatcttttgc
    45001 tggttcgggc tggcaaaaat tggcgcgatt atggtgccga ttaacgcccg cctgttacgc
    45061 gaggaaagcg agtggatctt gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa
    45121 ttttatccta tgtatcaaca gattcagcag gaagatgcca ctcaattgcg gcacatttgc
    45181 ctgacagatg tggcacttcc cgctgatgat ggagtgagtt catttactca actgaaaaat
    45241 caacaacctg ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt
    45301 ctcttcacct ccggcaccac ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg
    45361 cgcttcgctg gatattactc cgcctggcag tgtgcactgc gtgacgatga cgtctacatg
    45421 acggtaatgc ctgcgtttca tatcgattgc cagtgtactg cggcgatggc ggcgttttct
    45481 gccggggcca cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg gggacaggcg
    45541 cagaagtacc gcgccaccat taccgaatgt attccgatga tgatccgtac gttgatggtg
    45601 cagccgcctt cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac
    45661 ttgtcggagc aggaaaaaga cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct
    45721 tatgggatga cggaaaccat tgtgggcatt atcggcgatc gccctagcga taaacgacgc
    45781 tggccgtcga ttggtcgggc ggggttttgc tacgaagcgg agatccgcga cgatcacaat
    45841 cgcccgctcc cggcaggtga gatcggtgaa atctgtatta aaggcgtacc agggaaaacc
    45901 atcttcaaag agtattttct caacccgaaa gccactgcga aagtgctgga agccgacggc
    45961 tggctacata ccggcgatac cggataccgc gacgaagaag gcttttttta tttcgtcgat
    46021 cgccgctgca atatgatcaa acgcggtggc gagaatgtct cctgcgtgga gctggaaaat
    46081 atcatcgcca cccatccgaa aattcaggac atcgtggttg tgggtattaa agattcgatt
    46141 cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag
    46201 gaattttttc gcttctgcga acaaaatatg gcgaaattta aagtgccctc ttatctggag
    46261 atcagaaaag atctgccacg taattgctcg gggaaaataa ttagaaagaa tctgaaataa
    46321 atgaaacgtc agggaactac cctgccagct aataatcacg ccctaaaaca atatgcattt
    46381 tttgcaggga tgttgtcgtc cctaaaaaag caaaaatgga gaaaaggaat gagtgaatca
    46441 ttacatctga cccgcaatgg atcaattctg gaaattaccc ttgatcgccc aaaagcgaat
    46501 gctattgatg caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat
    46561 ccgcaattac gtgtcgccat tattaccggt gccggagaga aattcttttc cgcgggctgg
    46621 gatttaaaag cggcagcaga aggcgaagca ccggatgctg actttggccc gggtggtttt
    46681 gcgggattaa ccgaaatttt caatctcgac aaaccagtta tcgcagctgt gaacggctac
    46741 gcctttggcg gcggtttcga actggcgctg gcggcagatt ttattgtttg tgccgataac
    46801 gccagcttcg ccctgccgga agccaaactg ggcatcgttc ctgacagcgg cggtgtgctg
    46861 cgtctgccga agatcctgcc gcctaccatc gtcaatgaaa tggtgatgac cggcagacga
    46921 atgggcgcag aagaggcgct gcgttggggg atagtcaacc gcgtggttag ccaggcggaa
    46981 ctgatggata acgcccgcga actggctcag cagctggtta acagcgcccc gctggcgatt
    47041 gcggcgctga aagagatcta ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc
    47101 tatattcgca gcggcgtgtt gaaacactat ccatcggttc tgcattcgga agatgccatt
    47161 gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg
    47221 tgagctatta cgcctttgag gggttaattc cggtggttca cccgacggcg tttgtccatc
    47281 ccagtgccgt cttgattggc gatgtgattg tgggagccgg tgtctacatc ggcccactcg
    47341 cctcactgcg tggtgactac gggcggttga tcgtgcaagc gggagccaat attcaggatg
    47401 gctgcattat gcatggctac tgcgacactg acaccatcgt tggggaaaac ggccatatcg
    47461 ggcatggagc gatcctgcat ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca
    47521 gcgtaattat ggatggcgcg gtcattggcg aagagagcat tgttgccgca atgagctttg
    47581 tcaaagcagg ctttagtggc gagaaacgcc agttgttgat gggtacgcca gcccgcgctg
    47641 tacgcagtgt tagtgacgac gagttacact ggaaacggtt gaataccaaa gagtatcagg
    47701 atcttgtcgg gcgctgtcat gcatcgttac atgaaacgca gccactgagg caaatggagg
    47761 aaaatcgccc ccgtttgcag gggacgacgg atgtgacgcc taaacggtga tcttgccgga
    47821 tgcgacctgg ttgctggata agatgctaca acatcgtatc tggcaagcag gacgcccggt
    47881 atcaacgaga taaaattaac gacgcatact ctttgacaac atcatctgcc acttctgctc
    47941 gcggttaagt tccgtgagcg gaacggcagg aatacgcggt gtctttctta cgccgactaa
    48001 tttttcccga ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt
    48061 aaccagtcgc tgacactggc atccccgccc ttccagctta ttagggatca tcttaacttc
    48121 gcagcttatt tctgtgactt ccgacagaat ataagtcagg gtattaaccg ccttgctgtg
    48181 ttcaatatcg aaatgaatag agatctctct tgctatcacc caccgatttt cagccatcat
    48241 ccattcggcg attaacaagt agagtggttt ttcaacatat ccttcacaca tacgaactcc
    48301 agtttcacaa attaaaagtc tggctatgac tcgttttgaa gcaattaaac aagaccatat
    48361 taaaattgtg gatatatcga tagtatgcaa cttcaccgtc gataagtgtg agctaaagct
    48421 tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaaacggtc
    48481 ctcatcagag aaccgtatta gttattcgat caaattagcg gatgaaaaat atccgccacg
    48541 acacgctatt atttgatctg tgcgtgcatt tcctgcaccg aaattacttt ttcagtcgca
    48601 tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca gggtggtgtc ataatgcact
    48661 ttatattgca gcgcactgcg acggatcacg cgggagtctt caatcgcacg acggcctgag
    48721 gtggtgttga tgatgtaggt atattcgcca ttcttgatac ggtcctgaat gtgcggacgg
    48781 ccttcatgca ccttgtttac cagacgcgga ttgatacccg cttcgcccag cacaatcgcc
    48841 gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc
    48901 acgcgttctt tatcgccttc gcgcacggaa agcagcgcac gaccgtgttt cttcatggtg
    48961 gagttgctgc ccagctgcgc tttggcgaac gcttcagcga aggtgcggcc cacgcccatg
    49021 acttccccgg tagagcgcat ttctggccct aacagcgggt caacgcctgg gaatttattg
    49081 aacggcagca ccacttcttt caccgagtag tacggcggaa taacttcttt ggttacgccc
    49141 tgctcagcca gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg
    49201 gtggctttgg agacgaacgg aacagtacgc gccgcacgcg ggtttacttc aatcaggtaa
    49261 acttcgttgt ttttcaccgc aaactgcacg ttcatcagac cgcgtacctg caattcgaag
    49321 gccagtttct gcacctgctg gcgcatcaca tcctgaattt cctgacttaa ggtgtacgct
    49381 ggcagcgaac atgcggagtc accggagtgc acgcccgcct gctcgatgtg ctccatgatg
    49441 ccgccaatca gcaccatttc gccgtcgcag atggcatcca cgtcaacttc tactgcgtca
    49501 tcaaggaaat gatccagcaa cactggcgca tcgttagaca cgctaaccgc cgtctggaag
    49561 tagcgacgca ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa
    49621 gacggacgca ccaccagcgg gtagccaatc tcttttgcct tctcaactgc catttcaata
    49681 gcagtaacgg tggcgttcgc cggttgtttc agtttcagac ggtcaaccgc atgctggaag
    49741 cgttcacggt cttccgcacg gtcgatagca tccgggctgg tgccgataac cggtacgcca
    49801 gcggcttcca gtgcacgcgc cagtttcagc ggggtttgac cgccgtactg aacgataacg
    49861 cctttcggct tctcaatacg cacgatttcc agcacatctt ccagagttac cggctcgaag
    49921 tagaggcgat cggaagtgtc gtagtcggta gagacggttt ccgggttaca gttaaccata
    49981 atggtttcgt aaccgtcttc acgcagcgcc agcgaggcgt gtacgcagca gtagtcgaat
    50041 tcgataccct gaccgatacg gtttggaccg ccgccaagca ccatgatttt ttcacggtcg
    50101 gtagacggat tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg
    50161 gcaaactctg ccgcacaggt atccacgcgc ttgtagaccg ggtgcaggtc atattggtca
    50221 cgcagcttac ggatttccgc ttcgcgtacg cccgccagtt ttgccaagcg cgcatcggca
    50281 aagcctttgc gtttcagctg gcgcaggaat tcagcgttca ggccagtgat gcccacttcc
    50341 gccactttct cttccagacg caccagttct tcaatctgta ccaggaacca gcggtcaatg
    50401 ttagtcaggt tgaagacgcc gtccacagac aggcccgcgc ggaaagcatc ggcgatgtac
    50461 cagatacgct ctgcgcctgc gtctttcagt tcgcgacgga ttttggttaa cgcttccggg
    50521 tcatccaggc tcactttcgg gtcgaagcca gttgcgccca cttccaggcc gcgcagcgct
    50581 ttttgcaggg attcctgctg tgtgcgacca atcgccatca cttcgccaac cgatttcatc
    50641 tgagtggtca gacggtcgtt agcaccggcg aatttttcga agttgaagcg aggaatttta
    50701 gtaaccacat agtcgatgga cggctcgaag gaggccggag tacgtccgcc agtgatgtcg
    50761 ttcatcagtt cgtctagggt gtaacccacc gccagtttcg ccgccacttt agcaatcggg
    50821 aaaccggtcg ctttcgacgc cagcgccgaa gaacgggaca cgcgtgggtt catttcgata
    50881 acaatcaggc gaccgttttt cgggttcacc gcaaactgga cattggaacc accggtttca
    50941 acgccgattt cacgcagcac cgccatcgag gcgttacgca tgatttgata ttctttgtca
    51001 gtcagcgttt gggctggcgc gacagtgatg gagtcaccgg tgtggatgcc catcgcatca
    51061 aagttttcga tagagcagac gatgatgcag ttgtcgtttt tatcacgcac cacttccatc
    51121 tcgtactctt tccagccgat cagcgactca tcaatcagca actctttggt cggagagaga
    51181 tccagaccgc gggcgcaaat ttcttcaaac tcttcacggt tataagcgat accgccgccg
    51241 ctaccgccca tggtaaagga tgggcgaata atgcacggga agcccacgtc agcggcaaca
    51301 gccagcgctt cttccatcgt gtgtgcgata ccggaacgcg cggtttccag accaattttc
    51361 ttcatcgcta cgtcgaaacg acggcggtct tctgctttat caatcgcatc ggcagtggca
    51421 ccaatcatgg tgacaccgaa ctcttccaac acgccctgac gttccagctc cagcgcgcag
    51481 ttcagcgccg tctgaccgcc catggttggc agcaccgcgt ccgggcgctc tttttcaata
    51541 atcttgcgta ccacttccca gtgaatcggc tcgatgtagg tcgcatcggc catttccggg
    51601 tcggtcatga tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcgcgc
    51661 agggctttac aggcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc
    51721 gggcccgcac ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta
    51781 ctcctgatta cttagcggtt ttacggtact gctcaattaa cttgataaag tggtcgaaca
    51841 acggcgcggc gtcgtgtgga ccagggctgg cttcagggtg accctggaag ctgaacgccg
    51901 gtttatcgat acgatgaatg ccctgtaacg taccgtcgaa cagggattta tgcgtgacac
    51961 gcaggtttgc aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca
    52021 tcaccacgtt tttctccaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca
    52081 ttttgacagt cttcgcaccg ctcgccagcg ccagcagctg atgaccgaga cagatgccga
    52141 ataccggaat atcggtttcg aggaacttct ggatggcggt aatggcgtaa tcgcacgggg
    52201 ccgggtcgcc aggaccgttg gagaggaaga tgccgtctgg attcattttc agcacatctt
    52261 ccgcagaagt ttgcgccgga acgatggtca ggcgacagcc tctatccacc agcatccgca
    52321 ggatgttgcg cttggcacca aaatcataag ccacgacgtg gaacggcagc tcgtcttctt
    52381 ttttcgcttc tggcaggccg ccggtcaacg tccagctccc ttgtgtccag ctataggctt
    52441 ctgcggtggt cacttctttt gccagatcca tgccgttcag acctgggaac gcgcgggctt
    52501 tttctaacgc cagcgccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc
    52561 ctttctcgcg cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt
    52621 tatggcgctt caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg
    52681 gcaggtcgcg aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat
    52741 tagtgccgac attgccaata tggggataag taagagtaac gatttgacga gaataggaag
    52801 gatcagtgag gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg
    52861 ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata
    52921 gcgctgactt aatcaaaaca ccctccatag aatattcact cactttattt gcatattaat
    52981 tcacagtgat gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg
    53041 gcattctgga gatatgggcg ctaaaagtca acttaatggc aggatttttt atattatttt
    53101 gtggctcatg catgttttac aggcattaag cgacaaaaac atcatctaac ctgttctgga
    53161 aaacgcttgc gcagcaagaa gattgcatga aataaaaacg agcaggaaaa agtggaccaa
    53221 atggtcaaaa tttacattaa aacaacaaaa atagaagacg aaacatgtaa tttttggcag
    53281 ttatagaaaa tttaaaagca taacaccaaa aataaaaggg ctaaaaatta aagcccttta
    53341 taattaataa attaaatgtg ttacttttgc atcataataa acattttgtg gttacaaatt
    53401 attgagatca agcacatctc gcatatcaaa aagaccactt tccttaccac tcaaccacaa
    53461 agccgatctt accgcaccgt tagcaaatgt catgcggctg gacgccttat gggtgatctc
    53521 cagacgctcg ccaatatcgg caaacatcgc ggtatgttcg ccaacgatgt cacctgcacg
    53581 cacggtggca aaaccaatgg taccaggcac acgttcaccg gtatggcctt cacgactgta
    53641 gaccgcgcaa tctttcagat ctttatcaag ggcgtgggcg atcgcctctc ccattgccag
    53701 tgcggtgcct gacggcgcat caactttatg tctatgatgt gcttcaataa tttcgatatc
    53761 ggtgtagtca cccatcactt tggctgcttt ctccagcagc ttaagcatga cgttaacgcc
    53821 aacgctaaaa ttggcggcaa agacaatcgc aatatcggcg gcggcgtcac gaattgcctg
    53881 tttaccggct tcgtcaaacc ccgtagtgcc gatcaccatc cctttgccat gctgccgaca
    53941 aaaagcgaga tggttcagcg taccttccgg acgggtaaaa tcgataaaca catcaaaatc
    54001 atcttttatc gcatcaagac tgctttgcac ggtaacgcct gttttccctg caccagccag
    54061 ctcaccggcg tcgctgccca gtaaagaaga tccttcgcgc tccagcgccg cgcccagctg
    54121 cacgccttct aatgccagtg ccgcctgaat caactggcgg cccatacgcc ccccggctcc
    54181 cgcgatggca acgcggatgt ttgcatcatg catagctatt ctcttttgtt aatttgcata
    54241 gaccgttttc agagtaacca gcgcatcgct acgccgccag ccaaaaacgc tgataattag
    54301 aaattaatga taaacaggga gggatatcag tgaaaggcat gagtcgatga atgactgcat
    54361 gccgataaca tgtgacaggt tacggcacca gagccagcac ctcagccacc cactgttgga
    54421 agccttttac attcagatcc aatgccacct gtacattggc tggtttgccc aggcaaccgt
    54481 cgatatcaac caccgtcgtg cctgaggtaa attcgccttg agtttctacc gcgacaaaac
    54541 acggcttgag ggtgaataag tccgggcgca ccagccaggc gatggcgcag agatcgtgca
    54601 ttcgcagacc gctttgcata ctgccgctac ggtagtggct aaacagggcg tggagcattt
    54661 ttccggtgcg attcaactcc ggtaaagtag cgagatagtc aggggttaat attgcctgat
    54721 tggtgacatc cagaccgcac atgacaattt cgatgccgct acggaagaca caggcagccg
    54781 cttctggatc ggcagcgata ttaaactcgg catttggtgt acagttgccg cgaccagcag
    54841 aaccgcccat gatcaccaga cgacgaatat acggtttaca ttccgggcat tgtaaaagta
    54901 acagcgcaat attggttaac gggccgatgg ccaccagggt aacgggctct ggtgcacgca
    54961 tcagggcatc ccgaatcgcc agaaacgccg gtatcccgag tggctttcgg ttgtgctcaa
    55021 caaagtcgta gccagccatt cccgattcgc cgtgcacaga tgctgcatca cgcggtgcgc
    55081 gtaccagcgg cacagcggct ccctgggcga gcggaatctc cgcattccag aaatgcagca
    55141 gttgcagggc attgcgggta gttttctcaa ccgagacatt acccgcgacg gtggtcatca
    55201 gttgcaggtc gagttcgggg gcaaaaatcg cggcggcaat ggcgacggca tcgtcaatgc
    55261 cggggtcagt atcgaggaag ataggtaaac gcatgttttc ttcataaaaa atgccggtaa
    55321 caagaccggc attttcgcat aacttaggct gctaatgact taatcgactt cacgaatatc
    55381 gacacgcagc tctttcggca cttcgaaaac aatattttct tcacggcctt ccagcggaat
    55441 ggcttcacca ccacccaact gctgcaaacg tgccaccaca ttctgcacca gaatatccgg
    55501 agccgatgcg cccgcagtca cgccgacgca tttaacctct ttcacccact cttcctggat
    55561 atctttcgca tcgtcaatca aaaacgcgtg tttgcccata cgctgggcca gctccgccag
    55621 acggttggag ttggaggagt ttttcgaacc gaccaccaac acaacttccg cctgttctgc
    55681 cagggcgcgt accgcttcct gacggttagt cgtggcgtag cagatgtcat ctttgcgcgg
    55741 accgacaatt ttcgggaagc gtttacgcag cgcgtcgatc acatcagacg tatcatccac
    55801 cgacagcgtg gtttgggtca taaaggagag cttctcttcg tttttgaccg tcagtttcca
    55861 cacatcgtct ggcgattcga ccagatacat tcccccttcg gggttgctgt actgccccat
    55921 cgtcccttcc acttccgggt gcccggcgtg gccgatgaga atagattctt cgccacggcg
    55981 actggcgcgg gcaacttcca tatgcacttt ggtcaccagc ggacaggtgg catcaaacac
    56041 cgtcaaatcg cggcttttcg cttcgttacg aaccgcctga gaaacaccgt gtgcggagaa
    56101 aatcaggatc gcgccgtccg gcacttcgct aatctgctca ataaagatag ccccacgctc
    56161 gcgcaagcta tcgaccacgt agcggttatg caccacttcg tgacggacat atatcggtgc
    56221 gccgtaaatg gccagcgcgt tttcaacaat gctgatagcg cggtctaccc cggcacaaaa
    56281 accacgcggg ttggccaaca ggatctgcat gttacgcctc cagtgccgga tcgatttcca
    56341 gcacttcaat atcaaaatga acggtctgcc cggccagcgg atggttgaaa tcaacggtaa
    56401 tggagtcgcc gttaatttcg cggatcacac caggcatctc actgccatcc attgcggtaa
    56461 aaagcatgat tgcgccaatt tctggctcgc ctgcatccat aaattcacgg cgggagaagt
    56521 actgaatcag gtccggtgac ggcacgccaa acgccgcatc tggctccagc gagaaggtgg
    56581 ttttgtcgcc cactttcagc cccagcaggt gttgctccag cccttcagaa agagaagcat
    56641 cgcccaggcg gaacagcgcc ggtttaccgt tgttgcgagt agactcggcg gtggtgccat
    56701 cgtcaagttt tagcgtgaag tgcaccagga cggcgctatt gctctgtaca gattcagaca
    56761 tgcaggtttg ctcttatttt tgaggatttt tgccggatgc ggcctacgat ttcgtagcaa
    56821 tctgtaggcc gcatccggca gggtttatta ttgttttttc gctttggaag gtaaaaaccc
    56881 ttccagcacg atcagtgccg caccgacaca aatggcagta tcggcaaggt tgaaggtggc
    56941 gaagtgccag tcgccgacgt agaagtcgat catatcgaca acgaagccgt gccacaggcg
    57001 gtcgaacagg ttgcccagcg cgccgccaat aatcagcgcg taagcgatat tgtttagctt
    57061 ctgcgtggcc ttcgagcgat acatcatcac tgccaggagc acgctaatac caatcgcaat
    57121 accggcaaag aaccaacgct gccagccgcc gctatcggca aggaaactaa acgccgcgcc
    57181 atagttacgc gcataatgca gattaagcga cgggaacagc gggaccgtat cccccagagc
    57241 aaagttctgg aggatcaggt atttgctgcc cagatcgata atcagcacga ctaccaccag
    57301 ccacagccag cgtagccctg ttgaacagat cgattgactc atcaggcaaa cttacgtttt
    57361 tcaccgtcac cggcgacgtt gctgacacag cggccgcaga tttctgcgtg ttccgccacc
    57421 ttgccgacat cctgggtgta gtgccagcag cgtgggcact tctcaccttc ggctttactc
    57481 aacgcgactt tcagcccttt gagtacttcg ctctgctgag catcagcagg tgcgtcgtta
    57541 tagtctgcaa cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc gcccagcgcg
    57601 gtcagtttcg ccgccagttc cggttctgca tacaaggtta ctgccgcttc cagcgagcca
    57661 cccactttct tgtcggcacg cgcttgctca atgactttgt tcacttcgcc acgcactttc
    57721 aacagctcgt cccagaacgc atcgttcatt gcttcactgt ctgccagacc aaacaggcct
    57781 tcgtaccact caccggtgaa gacgtatttt tcacgttcgc ccggcagata gccccacact
    57841 tcatcagcgg tgaaggagag gattggtgcc atccagcgca ccagcgcttc agcgatgtga
    57901 tacagcgcag tctggcagct acgacgcgcc acgctatccg ctttggcggt gtactgacgg
    57961 tctttgatga tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg catcagacgc
    58021 tgtaccactt cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc ctgtgccgct
    58081 ttcgcacaac ctacggccca gcgatccagt accaccatct cttccggttt caccatatct
    58141 tttgctggat caaaaccatt caggtttgcc agcaggaagc gcgcggtgtt acggatacga
    58201 cgatagctat cggcagcacg tttcaggatc tcgtcagaaa cggccatttc accggtgtag
    58261 tcggttgatg ccacccacag acgcagaata tccgcgccca gtttgttcat cacatcctgc
    58321 ggcgaaacgg tgttgccgat ggatttagac atcttgcgac cctgaccatc cacggtaaag
    58381 ccgtgggtca gtacctgacg atacggcgct ttgcctttca tcgcggtgga gatcattagg
    58441 gaagacatga accaaccacg gtgctggtca gaaccttcca gatacatgtc cgctgcgtga
    58501 ccggcaaatt ccggacgcac gtcaacaaca gaagagtggg tagatccgga gtcaaaccat
    58561 acgtcgagag tatccggcac tttcacatac tggtcagctt cgtcgccgag gatctctttc
    58621 gcatcgagat cccaccacgc ctggatgccg tcaacttcaa cgcgtttagc cacttcttcc
    58681 atcagttcga gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa cagtgacatc
    58741 ggtacgcccc aggtgcgctg acgggagata caccagtcag gacggttagc aaccatcgac
    58801 tcgatacgcg cctggcccca gtccgggatc cactgcacgc ctttgatctc tttcagtgac
    58861 tgcgcacgca gacctttctg atccatgctg acgaaccact gcggtgtcgc gcggaagatg
    58921 atcggcgttt tgtgacgcca gcagcacgga tagctgtgct gcattttctc aacgtgcagc
    58981 agcgcgcctt tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa gacgttcacg
    59041 ccatccagcg tcggataagt gcccggcaga taagtgccgt ccgggccaac cgggttagcg
    59101 gtttccaggc cgtatttctg accgatcaca tagtcgtccg ggccgtggcc aggcgcggtg
    59161 tgaacggcac cggtaccggc atccagggta acgtgatcgc cgaggattgc cggaacgtcg
    59221 aagcccataa acggatgggt aaagcgcagc agctcaagct ctgcaccttt taccgtgccg
    59281 agaatggtgt aatcggtcac gccgatacgc tgcattacgc tttcaaccag atctttcgcc
    59341 agaatcgcgg cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg tgcaatagag
    59401 attgcgcggt tcgcaggcag agtccacggc gtagtggtcc agatcaccag cgagattggg
    59461 ccgttaacgt tgcttacgcc aaatttcgct ttcagcgcat cctgatcgac cgcctggaaa
    59521 gcgacgtcaa tggacggaga agttttgtcg taatactcaa cttccgcttc tgccagtgca
    59581 gaacggcagt ctacgcacca gtgcaccggc ttcgcgcctt tgtgcaggtg accattgccg
    59641 atgattttgc ccagcgcgcg gatgatgttg gcttcagttt tgaagtccat agtcagatac
    59701 gggtgcgacc agtcgcccag cacgcccaga cggataaagt ctttgcgttg accgtcaacc
    59761 tgggtcgccg cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa tttctcaccc
    59821 ggcttaccgt attcttgctc tactttcagc tcgatcggca gaccgtggca gtcccagcca
    59881 ggcacatacg gcgagtcata accggaaagc cctttggact tcacgataat gtctttcaga
    59941 atcttgttaa ccgagtgacc aatatgaatg ctgccattcg cataaggagg gccatcatgc
    60001 agaatgaagg tttttttgcc ttttttagcc gcacggatga tgccgtacag atcatcatca
    60061 gtccaacgcg ccagcattcc cggttcgcgc ttggcgagat cgccacgcat cgggaaccct
    60121 gtttccggca aattcagggt tgatttatag tcactcatca gattctcggt tccgtgtttc
    60181 ggtttgatta cataacaggc ttaagccggt tttgttagcc caaaaaattc gcgggcggtt
    60241 aattcatcac gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg ctgctcattg
    60301 cgtatttttt tacgcagcac tacttgtata tggcgaccgt aaaggtccat tgcaacatct
    60361 aacaaatgca cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt tccgatgttt
    60421 gccacgccgg gtaacggctt ttcaccgagg cccagcactt ctaccgcata aacccctttc
    60481 accggggaaa cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat agtgcgccct
    60541 aattcatcac cgtggactac acgcccggag atggcaaacg ggtgccccag taaactttct
    60601 gccagagcca gattgtcatc agccagcgcc tgacgcacgg cggtgctgct gatacgcaca
    60661 ccaccttcgc aaaaagtttg cgtactggtg atatcgaagc cgtattccat gccagctttc
    60721 tgtaataaca agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc acctacggca
    60781 agaaatttta cgcgcaaatg cttcaccaaa agatcgctga tgaaattttg cgcggttaac
    60841 gccgcaaaac gcctgtcgaa acgcacgcac agcacgtaat caacgccaca ctctgcaagg
    60901 taacgcagtt tttcccgcag ccgggtcagt cttgccgggg ctttatcggt agcaaacagt
    60961 tccagtggtt gaggttcaaa aagcatcacc atcaccggta agttgcgctt gcgcccttct
    61021 tcctgcaagc cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa attaccaata
    61081 gtcagcacac acccttcttg cggggcctgg ctgagattat gtatgccgcg tatcagcttc
    61141 atgtctggct caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg tcaaggttaa
    61201 ccggcgattg agtaccgaaa tcagaaagaa gtggcttttc attgccatgg cgcaaatcac
    61261 gggaagaaac tgaccgcctg ctgcaatttt tatcgcggaa aagctgtatt cacaccccgc
    61321 aagctggtag aatcctgcgc catcactacg taacgagtgc cggcacatta acggcgctta
    61381 tttgcacaaa tccattgaca aaagaaggct aaaagggcat attcctcggc ctttgaattg
    61441 tccatataga acacatttgg gagttggacc ttggctaata tcaaatcagc taagaagcgc
    61501 gccattcagt ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat gatgcgtact
    61561 ttcatcaaga aagtatacgc agctatcgaa gctggcgaca aagctgctgc acagaaagca
    61621 tttaacgaaa tgcaaccgat cgtggaccgt caggctgcta aaggtctgat ccacaaaaac
    61681 aaagctgcac gtcataaggc taacctgact gcacagatca acaaactggc ttaatcgcca
    61741 atttgctgaa gctttgtgaa aaagcccgcg caagcgggtt tttttatgcc tgctgctttt
    61801 gtgatgcgtg agagaacaca ctgccggttg ccagatagtc aaaagagaca tgccgttgta
    61861 ctatctgaac tcttcgtggc ttaagaatcg tagaaccatc ggctgataca ttctgaacaa
    61921 gaatatctac tcaatctcca gactccccca caaagaatat ggatattgtg atacacattg
    61981 aggtattaaa cttaagacca ctctattttt gaattaaatt cctatatact ttaaccattc
    62041 ataaatatct cctcattaga cctt
//
LOCUS       DADLXX010000008        60685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLXX010000008 DADLXX010000000
VERSION     DADLXX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.008"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..320
                     /locus_tag="JNX27_000489"
     CDS             <1..320
                     /locus_tag="JNX27_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7881817.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JNX27_000490"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JNX27_000490"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7881818.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JNX27_000491"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JNX27_000491"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7881819.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVGLA"
     gene            complement(2164..3066)
                     /locus_tag="JNX27_000492"
     CDS             complement(2164..3066)
                     /locus_tag="JNX27_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7881820.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JNX27_000493"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JNX27_000493"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7881821.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JNX27_000494"
                     /pseudo
     CDS             complement(3724..4953)
                     /locus_tag="JNX27_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5017..5457)
                     /locus_tag="JNX27_000495"
     CDS             complement(5017..5457)
                     /locus_tag="JNX27_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7881822.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JNX27_000496"
     CDS             complement(5562..6332)
                     /locus_tag="JNX27_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7881823.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JNX27_000497"
     CDS             complement(6329..7255)
                     /locus_tag="JNX27_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7881824.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JNX27_000498"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JNX27_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7881825.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNX27_000499"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNX27_000499"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7881826.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /locus_tag="JNX27_000500"
     CDS             8809..11199
                     /locus_tag="JNX27_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7881827.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNX27_000501"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNX27_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="JNX27_000502"
     CDS             12961..13308
                     /locus_tag="JNX27_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7881828.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="JNX27_000503"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="JNX27_000503"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7881829.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="JNX27_000504"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="JNX27_000504"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7881830.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="JNX27_000505"
     CDS             complement(15128..15490)
                     /locus_tag="JNX27_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7881831.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNX27_000506"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNX27_000506"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7881832.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIINRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JNX27_000507"
     CDS             18618..20471
                     /locus_tag="JNX27_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY7881833.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="JNX27_000508"
     CDS             complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="JNX27_000508"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7881834.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPNPNPKAKKK"
     gene            complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="JNX27_000509"
     CDS             complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="JNX27_000509"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7881835.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="JNX27_000510"
     CDS             complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="JNX27_000510"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7881836.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="JNX27_000511"
     CDS             complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="JNX27_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7881837.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28345..29718
                     /gene="aroP"
                     /locus_tag="JNX27_000512"
     CDS             28345..29718
                     /gene="aroP"
                     /locus_tag="JNX27_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7881838.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="JNX27_000513"
     CDS             complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="JNX27_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7881839.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="JNX27_000514"
     CDS             complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="JNX27_000514"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7881840.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31251..32144
                     /gene="nadC"
                     /locus_tag="JNX27_000515"
     CDS             31251..32144
                     /gene="nadC"
                     /locus_tag="JNX27_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7881841.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32347..32787
                     /gene="ppdD"
                     /locus_tag="JNX27_000516"
     CDS             32347..32787
                     /gene="ppdD"
                     /locus_tag="JNX27_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7881842.1"
                     /translation="MDKQHGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32797..34182
                     /gene="gspE"
                     /locus_tag="JNX27_000517"
     CDS             32797..34182
                     /gene="gspE"
                     /locus_tag="JNX27_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY7881843.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34172..35374
                     /gene="hofC"
                     /locus_tag="JNX27_000518"
     CDS             34172..35374
                     /gene="hofC"
                     /locus_tag="JNX27_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7881844.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="JNX27_000519"
     CDS             complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="JNX27_000519"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7881845.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36677..37297
                     /gene="coaE"
                     /locus_tag="JNX27_000520"
     CDS             36677..37297
                     /gene="coaE"
                     /locus_tag="JNX27_000520"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7881846.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37297..38040
                     /gene="zapD"
                     /locus_tag="JNX27_000521"
     CDS             37297..38040
                     /gene="zapD"
                     /locus_tag="JNX27_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7881847.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38050..38247
                     /gene="yacG"
                     /locus_tag="JNX27_000522"
     CDS             38050..38247
                     /gene="yacG"
                     /locus_tag="JNX27_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7881848.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="JNX27_000523"
     CDS             complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="JNX27_000523"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7881849.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38796..41501)
                     /gene="secA"
                     /locus_tag="JNX27_000524"
     CDS             complement(38796..41501)
                     /gene="secA"
                     /locus_tag="JNX27_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7881850.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41563..42075)
                     /gene="secM"
                     /locus_tag="JNX27_000525"
     CDS             complement(41563..42075)
                     /gene="secM"
                     /locus_tag="JNX27_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7881851.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="JNX27_000526"
     CDS             complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="JNX27_000526"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7881852.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVCVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="JNX27_000527"
     CDS             complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="JNX27_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7881853.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="JNX27_000528"
     CDS             complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="JNX27_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7881854.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="JNX27_000529"
     CDS             complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="JNX27_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7881855.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46627..47547)
                     /locus_tag="JNX27_000530"
     CDS             complement(46627..47547)
                     /locus_tag="JNX27_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7881856.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47540..49015)
                     /gene="murC"
                     /locus_tag="JNX27_000531"
     CDS             complement(47540..49015)
                     /gene="murC"
                     /locus_tag="JNX27_000531"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7881857.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49069..50136)
                     /gene="murG"
                     /locus_tag="JNX27_000532"
     CDS             complement(49069..50136)
                     /gene="murG"
                     /locus_tag="JNX27_000532"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7881858.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="JNX27_000533"
     CDS             complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="JNX27_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7881859.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51377..52693)
                     /gene="murD"
                     /locus_tag="JNX27_000534"
     CDS             complement(51377..52693)
                     /gene="murD"
                     /locus_tag="JNX27_000534"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7881860.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="JNX27_000535"
     CDS             complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="JNX27_000535"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7881861.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53772..55130)
                     /gene="murF"
                     /locus_tag="JNX27_000536"
     CDS             complement(53772..55130)
                     /gene="murF"
                     /locus_tag="JNX27_000536"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7881862.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55127..56614)
                     /gene="murE"
                     /locus_tag="JNX27_000537"
     CDS             complement(55127..56614)
                     /gene="murE"
                     /locus_tag="JNX27_000537"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7881863.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56601..58367)
                     /locus_tag="JNX27_000538"
     CDS             complement(56601..58367)
                     /locus_tag="JNX27_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7881864.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="JNX27_000539"
     CDS             complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="JNX27_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7881865.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="JNX27_000540"
     CDS             complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="JNX27_000540"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7881866.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="JNX27_000541"
     CDS             complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="JNX27_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7881867.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14537 a        16811 c        14921 g        14416 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcgg gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggctccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc taggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatac cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccatgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcaatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttaa taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cacgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcgggttcgg
    20581 gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca cagactcgtg
    20641 cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac ccatttcgat
    20701 tgccagcccg atttcaccca gcagctcgcc gccgttggta ccaacaatcg caccaccgat
    20761 cacgcggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc agtcagaagc
    20821 gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcatagctga tgcctttctc
    20881 tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat aggcgatgga
    20941 cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag cggcaacgtg
    21001 accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc cgatagcaaa
    21061 gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac
    21121 ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac caatcgctac
    21181 cagcacggcg tcgtaacgct gcggttcagc tggtgctttt ttgccttcca tcgtcacata
    21241 aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca ggttgaattt
    21301 cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg ggataacctg
    21361 gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtaaacgg tgcccatttc
    21421 cagaccgatg ataccgccac ccattaccag caggcgttct ggcacttctt tcagttccag
    21481 cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca gttggatcgg
    21541 gcgagaaccc gctgcaatga tcgcattgtc gaagttgatc acggttttgc cgttctcacc
    21601 ttcaacttcc agggtgttag ccccggtgaa tttacccaga ccgttgacca ctttgacttt
    21661 gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt tctctttcca
    21721 ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag acgataccgt gttcagccag
    21781 cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag ggatacagcc
    21841 gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg tttccagacc
    21901 taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa gtaccacgac
    21961 ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg cggtcatacc
    22021 cgtcgtcttt caggccgcag cggcgttagc ttcacgccct caccccagtc acttacttat
    22081 gtaagctcct ggggattctg gcgcttgcta ccttgctgca cccagaaatc catagggtat
    22141 gtgaagtaat accctaacca ccaccgggtc gttctatccg tcggtatttt acaaaattgt
    22201 taacaatttt taaacaacaa acggcaaccg atttgtctat tcgctaataa cctcaataca
    22261 ttcatgagat taccagaaaa aagccggccg ttgggccggc tcttttactt acatcaccag
    22321 acggcgaatg tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc
    22381 gtcgatcacg cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc
    22441 tttaccattc cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg
    22501 cgcgttcaca atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa
    22561 gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt
    22621 catcagctcg cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccggaac
    22681 aaccagaccg ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag
    22741 acgctgaccg tcttccgaca gcgaactatt gaagcgaggc atctgctcaa gagctgcagc
    22801 aacggctttc atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc
    22861 ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg
    22921 ggtaacatgc gggatcatca cccagttacg gctcaggtta gcaccagaaa ttttctggat
    22981 acggcccagt tccacttctt cgatttcgcc aaacttgctg aagtccacct tcggccacgg
    23041 cagcatgcca gggataccac cgccagtcgc cgccggagct gcttctgcac gtttgatagc
    23101 ttctttcacg taagcctgaa cgtcttcgcg caggatacga cctttacggc cagtgccctt
    23161 cactttcgcc aggttaacgc caaactcgcg tgccagacgg cggatcagcg gggtcgcgtg
    23221 aacgtaagcg tcgttttcag caaattcaga tttgccttcc gctttcgcag ccggagctgc
    23281 tgccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc
    23341 tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgaaccag ttttcacttt
    23401 atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg gaacttccat
    23461 agaagcttta tcgccttcta cggtaatcag tgactgttca gcggcaactt tgtcgcccac
    23521 tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc
    23581 tttcacgcca gctgctggtg cgggggtcgc tgccggagtc gcttcctgtt tagccgccgg
    23641 agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc cggtagacac
    23701 tttgtcaccc acgttcactt tgatctcttt cacggtacct gcaaacggag ccggaacttc
    23761 catagaagcc ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc
    23821 aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac
    23881 gtcttttgcc gccgcagccg cgggtgctgc tgccggagct gcttctttct tctcttctgc
    23941 ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc
    24001 ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga
    24061 tggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac
    24121 tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg
    24181 tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta
    24241 tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca
    24301 cgtttagcca gttcgcccag cgccgcaacc acgacataag aagcatcaac ttcgaagtgg
    24361 tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcgatag
    24421 tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat
    24481 gccactgccg gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc
    24541 ggatgcagca tgttccagcg ttcacaatcc tgaccatcac gcgccagctc ggtgaaggag
    24601 gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca
    24661 cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt gctaccttca
    24721 atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc
    24781 ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca
    24841 ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc gtaagccggg
    24901 tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc
    24961 tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc
    25021 gcttgctggt ctccagccgc ccagcacaga tcgccgatac gctggaagcc gaacatcgag
    25081 taatagatgt agaatgggat catcggcaga ttgttcgtgc tgtaagaggt cgccgctgcc
    25141 agccaggaac accctgcgcc cagctcgttg atcccttcct gcagaatctg acctttctcg
    25201 tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg
    25261 ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg
    25321 atgatcggta ccagacgatc tttgatcgac ttgttcttca gcatcacgtt cagagcacga
    25381 acaaaagcga tagtggtaga gagctctttg ctctgctctt ccagcagcgc gccgaagtct
    25441 tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg
    25501 tgcagtttct gacgctgagc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg
    25561 atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggatg
    25621 tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc
    25681 gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc tttgcctttg
    25741 gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc
    25801 agtgcccaga tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta
    25861 ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg
    25921 tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttca
    25981 tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct
    26041 tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta
    26101 cagttgataa cgaaaaccag gttatccagt ttttcacggg tagcgatggt gatcgcacct
    26161 ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag
    26221 gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca
    26281 atcggaccca gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg
    26341 tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc
    26401 tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc ccggggagat gtggccctgg
    26461 aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac
    26521 acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag ttcgaggtct
    26581 tttttcgacg cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg
    26641 cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg
    26701 tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc
    26761 agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc
    26821 gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg
    26881 ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc
    26941 gccagaaagc tcaataagac aggttctacg tttagttacc gcgcttttat atgcgcttga
    27001 tttacaacat cttctggata atttttacca gaaaaatcac taattctttc gttgctccag
    27061 acgacgcaga gaacgctcac ggcggctctc ttcacggctt ctgtcgagca aaatttcttc
    27121 gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat
    27181 cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata
    27241 gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag
    27301 atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc
    27361 gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc
    27421 acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc
    27481 ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt cggagagcag
    27541 ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc
    27601 ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg
    27661 acgggagacg tcgaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg
    27721 gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg
    27781 ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg
    27841 gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt atgtccatac
    27901 aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt
    27961 caaagttgta actatgcaca aatgtagact ttacgtagga aagggatttt ttaacgatta
    28021 ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttttgat ttttaagtca
    28081 ataaaaacga atttaaattc actctgcata ttgagagggg ttgaggctga gctttacaaa
    28141 cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat tcgctgccgc
    28201 ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaactt ccttgatgta
    28261 aataaatcaa tacaacaaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa
    28321 aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag
    28381 cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggacaggg
    28441 ctattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac
    28501 gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa
    28561 gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt
    28621 ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc agagctgact
    28681 gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt ttctgccgcc
    28741 gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt tggcgagatg
    28801 gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat cttcggcggc
    28861 tggctgctgt tcagtggcaa cggcggcccg caggcgagcg ttagcaacct gtgggatcaa
    28921 ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga tgatggcgat tatcatgttc
    28981 tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa
    29041 agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta tattggctcg
    29101 ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac cagtccgttt
    29161 gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa catcgtggta
    29221 ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt
    29281 ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa acgcggcgta
    29341 ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt gattaactat
    29401 cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc actggtgatc
    29461 aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca ggaacaaggc
    29521 gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt
    29581 atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg
    29641 atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa
    29701 gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg gcaataatct
    29761 ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca cgaacgaggt
    29821 tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg
    29881 cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtat ggaaatcacc
    29941 cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg catataccac
    30001 acccgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa tgccggactg
    30061 taaacgatga tgcggcgtct ggtatctcgc cagccagtat tgccatgcac gcaagaacgc
    30121 ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc
    30181 aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg
    30241 ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg
    30301 ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt
    30361 acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg gaacgttgaa
    30421 caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa taatggtcat
    30481 gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg
    30541 atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt
    30601 tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc ccaatcaaat
    30661 gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttatta
    30721 gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc
    30781 gcatcggtat acgccagagt atcggtgcct tcaagctcaa tcccaataga aaaatcattg
    30841 cagcgttcac gcccctgata ctgagagact cccgcatgcc aggcacgttt atcgaaagga
    30901 acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac gcgcaaatgg
    30961 gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg
    31021 atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg
    31081 ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccaacc
    31141 agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc
    31201 agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc
    31261 gccgctacaa ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc
    31321 ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg
    31381 acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg atcacccgcg
    31441 agaatggcgt attttgtggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg
    31501 acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc aatcaaccct
    31561 tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact gcgcttaatt
    31621 ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttactggaag
    31681 gcaccaacac gcagttgttg gatacgcgca aaaccttacc tggcctgcgt tcagctctga
    31741 aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct gatgccttcc
    31801 tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt
    31861 cctggctgca tccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg
    31921 aggccctgaa agccggagcc gatatcatca tgctggataa cttcgaaaca gaacagatgc
    31981 gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg
    32041 acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc ggcgcgctaa
    32101 ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaattgctg acagatttat
    32161 ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac gcctcgcaaa
    32221 tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc cgtttcgttt
    32281 tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa tcaaggagcg
    32341 aaacagatgg acaagcaaca cggttttaca cttatcgaac tgatggtggt tattggcatc
    32401 attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca
    32461 ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg
    32521 gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc
    32581 acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac cgggcaagaa
    32641 agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc
    32701 ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat
    32761 gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca gctcacggcc
    32821 ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt tcatgttgcg
    32881 gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac caccaaacgt
    32941 attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag
    33001 acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct gactcgaacg
    33061 ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc
    33121 taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat
    33181 gccggagtcg cattaacagc cagattaaaa gtgctgggaa acctggatat tgcggaacat
    33241 cgcctgccgc aggacggcca attcactgtc gaactggcag gaaacaccgt ctcatttcgt
    33301 attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg
    33361 agccaagcac tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct
    33421 catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa
    33481 acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa tatttgtagc
    33541 gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt
    33601 gccgggctca cctttcaggg tgttttgcgt gcgttattgc gccaggatcc tgacgtcatc
    33661 atgatcggag agatccgcga tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc
    33721 ggtcacctgg tgttgtccac gctacacacc aattccacca ctgaaacgct ggtacgttta
    33781 cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt aatagcccag
    33841 cgtctggtac gtaaactttg cccacattgt cgccgacagc aaggggagcc catccatatt
    33901 ccagacactg tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc
    33961 taccacggtt tttatggtcg taccgccttg tttgaagtcc tgcccattac accagccata
    34021 cgccagctta tttccgctaa tactgaagtt gaatcgctgg aaacgcacgc ccgacaggcg
    34081 ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt
    34141 gaagagttaa tccgcgtact ggggatgccg catggcaagt aagcaactct ggcgctggca
    34201 tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga gccgtacttt
    34261 actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc gaatcgccat
    34321 caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct
    34381 acttaaagcc gggttaacgc tttctgaagg gctggctctg ctggcggaac agcatcccag
    34441 taaacaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt
    34501 ttccaatgcc ttattaccct ggtcagaggc atttccgccg ctctatcagg cgatgatccg
    34561 cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa
    34621 atcccagcgt cagttgaccg acaaagtgaa atcagcgttg cattatccca tcatcatttt
    34681 agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc
    34741 tatctataag accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc
    34801 agactttagt ggcgaatggg gctggctgct ggtgttgttc ggctttctgc tggcgatagc
    34861 caataagttg ctgatgcgcc gcccgacctg gcttatcgcg cggcagaaat tgctgttacg
    34921 catcccgatt atgggttcac tgatgagggg acaaaaactc acgcagatct ttacgattct
    34981 ggcgctgaca caaagtgcag gcattacttt tttacagggc gtagagagcg tcagagaaac
    35041 aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg
    35101 tcatcccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct gtttgcaatt
    35161 agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca
    35221 tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt
    35281 gctgatcata acgggaggaa ttatcggtac gctggtggtg gcaatgtatc tgccaatttt
    35341 ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacgccagcg
    35401 ttgggagatt acaggttgtt gaagatgcgg ttttcttgtt ccagcacacg aataaacgtg
    35461 gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg
    35521 ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag cttgacggtt
    35581 ttaccttctg ctgcgcggta ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg
    35641 gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg
    35701 ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag
    35761 gctttcgcca catcgcccgg cgtggtacag ccaccgtcgc tgacgatcat tccgcccaga
    35821 ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt
    35881 ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg
    35941 gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg
    36001 gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc
    36061 gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt
    36121 tcgaaatccg catcagacgt gccggtagaa accatcacat gtttcagcac atcagcggaa
    36181 gaattgttga taaacgcttg ccactcttcg acagaaaagt gtttatgcac agcagtcaga
    36241 atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc catatttgcg
    36301 gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc
    36361 agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta
    36421 aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata
    36481 agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact
    36541 gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa
    36601 cgtttcatca atttcacttt cgcgcgctca tttttaacgt tttgataatc atgatgaatt
    36661 cccgggaata taaattatgg ggtatatagt tgccttaacg ggaggcattg gcagtggtaa
    36721 gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat
    36781 tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg
    36841 cgaaaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt
    36901 cgccaacccg gaagagaaaa actggcttaa cgacctgctg catccgctga ttcagcaaga
    36961 gacgcaacac cagatccagc aagcaacttc cccctatgta ctgtgggttg tgccattgct
    37021 ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga
    37081 aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgaacatg tcgaacaaat
    37141 ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa
    37201 taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca
    37261 gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa
    37321 catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc
    37381 accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt
    37441 gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttaac
    37501 cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt
    37561 gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc
    37621 gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca
    37681 ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg
    37741 cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt
    37801 accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat
    37861 ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat
    37921 tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg
    37981 ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc ctgttgctaa
    38041 ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg
    38101 tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc
    38161 ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat
    38221 gactggagcg aagagccaaa gcagtgacat ttgtctgatg cctcacgtag gcctgataag
    38281 acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta
    38341 tccaacctac agacgcttaa gcttcgcaat taccggttca ttggctggcg gaaaatcatc
    38401 ggcattaaga ccgaccagcg acatccactc accgggttgc ccttctttac cccacggcac
    38461 cccttcccaa ctttcgacca gccagaacca cagagttata tgcctgtccg ggaattcata
    38521 ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacctctt cctgaagttc
    38581 acgcaccacc gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg gaaactccag
    38641 tttattcgcc atgtgcgcat ctgctgcgcg acgtgttata aagatttcat tgttctcgtt
    38701 gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg
    38761 cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc atggcactgc
    38821 ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt gcgttcaccg
    38881 gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct aagctgctgc
    38941 atttgtgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca
    39001 ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa cgactccagc
    39061 atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca
    39121 tagccacgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg ctctttccac
    39181 agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat ctcagcacca
    39241 accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg ctcacgcagc
    39301 gtctcttcat gcagttctgg ttctttatcc agccactcgg caattggtaa atcgaggtcg
    39361 aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagcgactgt
    39421 ggcggaatgt aggcatcaat ggtggctttg aacacatctt cacgaatgct gttgatggtt
    39481 tcgctcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga
    39541 tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acgactttca
    39601 actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat ggcttcgcct
    39661 ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc
    39721 agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa
    39781 cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc gatgatatgc
    39841 aggccacctg cttccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca
    39901 atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct acctccgagc
    39961 acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc cggataacct
    40021 gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac attgtgctta
    40081 ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga gatagtaccc
    40141 accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt
    40201 ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg
    40261 gtcggaacaa cgacggtatc cagtttgtag atggagctaa attcgaaagc ttcggtatca
    40321 gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc
    40381 gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg
    40441 tgcagaccat cggaccagcg tcggccctgc atggtacgac cggtgtgttc gtcaacgatg
    40501 ataacttcac catctttaac gatgtagtcg acgtcacggg taaacagcgc atgagcgcgc
    40561 agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag agactcccct
    40621 tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc acgttcggtc
    40681 aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg gaaggtttcg
    40741 gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt
    40801 tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg
    40861 atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcaggg
    40921 ctgaacgcca tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg
    40981 atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag gttgatacca
    41041 acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg
    41101 tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc
    41161 agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta
    41221 agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt
    41281 gcctcacgca ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc
    41341 agacgcgcac gaaactctgc ggttttccct ttcagttctt catcggagag tttttccatc
    41401 tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg
    41461 ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc ccaaacgccc
    41521 cgcgttacgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga ggcgttgagg
    41581 gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgtgg
    41641 ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcag
    41701 cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagattcttc
    41761 agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta ccgtgcgaat
    41821 agcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgcg tgttcgcttc
    41881 cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc
    41941 ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc
    42001 gaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt
    42061 cagtattcca ctcacgttat tgccatcccg atcgaattca aacgtattta gcgcacaaat
    42121 cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtgcg taaaagtgct
    42181 taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa aaaaacgact
    42241 ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa
    42301 tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc
    42361 tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta
    42421 ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg accacacatg
    42481 aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg
    42541 ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc
    42601 aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca
    42661 cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg
    42721 gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc
    42781 ttaaattcag cccacttatc gccatcttcg acacaaacag tctctttgat gcgaacaaat
    42841 tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac aaacggagcg
    42901 gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg
    42961 cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg
    43021 ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct
    43081 accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt
    43141 aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt
    43201 ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc aaccgaagtg
    43261 tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa
    43321 ttcttaatca gcttgcttac gtaggaatgc tgggatatcc agataatccg gctctttcgc
    43381 agtttgcggc gcattgtcat tcactacttt agcaaccggc ttctgctcct gggtcagcgg
    43441 agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac
    43501 cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag
    43561 ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc
    43621 aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc
    43681 cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga
    43741 agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca cgccagaacc
    43801 catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt ccacgttcat
    43861 caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttca gtacatcgtt
    43921 cgctgcgcca aacgcatcca gcaaggagat accgcggccc agaactttca gcagtttgtc
    43981 gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctcggc
    44041 gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag caacggtcag
    44101 gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac cggtaccacc
    44161 acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc
    44221 gcgatcctca tcagccgcat tgcggccaac ttctggatta gcgccagcgc ccagtccttt
    44281 ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc
    44341 atcggtattt accgcgaaga attcaacacc ttcaatacgc tcgcgcacca tgtgttcaac
    44401 agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc
    44461 cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg
    44521 tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga
    44581 gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt
    44641 cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa
    44701 accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc gctgagcaca
    44761 ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg
    44821 atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag
    44881 gttgagcaac tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact
    44941 ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc caacgatgga
    45001 acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa
    45061 ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg
    45121 caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca
    45181 gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa
    45241 tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat
    45301 atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacacccg aaagtcctac
    45361 cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac
    45421 acgatgctca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt ccacatcttc
    45481 ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg
    45541 cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg
    45601 gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc
    45661 cataccacgt gacgggcagc tgcccacgcc aatgatattg accataccgt cgggcagaac
    45721 ttcccctact aaagcggcaa ccttcgcggt accaatttcc agtcctacta ccagttttct
    45781 gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc
    45841 tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag
    45901 ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag
    45961 cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc
    46021 gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt
    46081 gccagcatct gccccatttc gcgatagccc tgcaacactt cattagcgct gccttccggg
    46141 ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt
    46201 ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga
    46261 tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg
    46321 cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt acccggctca
    46381 cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc
    46441 aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagctcaa cacgacccag
    46501 ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt
    46561 ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag agcagcctgc
    46621 gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc
    46681 ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc
    46741 cagcagataa aactgtccat cactgtccag cataacgtca atacgtcccc atcctttgca
    46801 acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttt
    46861 cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata
    46921 gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac
    46981 cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc
    47041 caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga
    47101 accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg
    47161 cttatcgctc aggccttttt caaactctgc gcgggttaac gctacccacg gcgcgaccgg
    47221 taaaccggca ccttgccaca gaagtttgct gcgtagttta tctattgaaa gcgcagatgc
    47281 catcactccg ctgccggtat aaggcaagcc catcagctcg agcatgccct gcagcgtacc
    47341 atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca tcgacttcag
    47401 ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca tcaataccgc cttcacgcag
    47461 cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg aagtcccgcc
    47521 caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt
    47581 tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca
    47641 ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgcaggg tccggcacca
    47701 gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc
    47761 ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg
    47821 tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa
    47881 acagcattac caggttttta tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa
    47941 cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca
    48001 ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc tggaagctct
    48061 ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag
    48121 ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc atcggctctt
    48181 tgtcctgacg cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac
    48241 gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg acgtcccaca cgcggtaaca
    48301 attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaaa ggcaggttgt
    48361 gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt
    48421 ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat
    48481 cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacccccg
    48541 ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc
    48601 tggaaaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac
    48661 gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat
    48721 gagctgcgac aatttccggg ttgtcggcag aaatggcgct ggaaacaacg accacgctgg
    48781 catcacgtac gttttccggg cgatggttga aataaatcgt cgcacccaga ttcagtaact
    48841 gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca
    48901 gaacttcggc aataccgccc ataccggcac caccaatgcc gacaaaatgt atgtgccgaa
    48961 cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt
    49021 acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc
    49081 agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc
    49141 gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc
    49201 atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag
    49261 cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc
    49321 tgccgcggcg atttcactca cagttaaagc accggagcgg caaacgacga catccgccca
    49381 cgcatacgcc gccgccatat catcaataaa ttcagtcact ttatgctgcg gttgccccgc
    49441 ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat gccagatagt
    49501 gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc
    49561 ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca aacgttgctg
    49621 cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt ccgcattagg
    49681 gaaagcgcct ggaaacgcct gcatcacttt ggtagcaatc ttcgccagcc atttattggt
    49741 taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg accacgcggc
    49801 cagaccacct ggacctgata catagccgcc cataccgagt accacgtcag gtttgtacgc
    49861 tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg cagctatcag
    49921 tgcttttata ccttttccac gcaaaccaga gatacgaatg aaatcaattt cgatgccatg
    49981 ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc
    50041 ctgagccatt agatagtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc
    50101 tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc
    50161 gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata
    50221 atcagtaagc tcgaaccacc gtaactgatt agcggcaatg tcagaccttt ggtcggtaac
    50281 atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa
    50341 caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttgcggcc aatcgacatc
    50401 gcacgaaaag cgacgaagaa taccattaaa agtgctagta ccacaccgac ataacctaat
    50461 tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt
    50521 tgtaccgaat tacctaaacc ttgcccccaa agttcgccgc gaccaaacgc catcagcgat
    50581 tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca
    50641 cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat gcccataccg
    50701 ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc
    50761 acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc
    50821 agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta
    50881 cgcaccagat agttggcgat atagcaaaac aacgacagtt ttgtcagctc cgcaggctgg
    50941 atacgcagca aaccgagatc gatccaacgc gatgcccctt taacagcgct acctactacc
    51001 agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag
    51061 aactccatcg gcagacgcag cgtaatgatc gccagaataa acgccaaaat cagatagaca
    51121 ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac
    51181 gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg
    51241 cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcccttt
    51301 agcgccgtgg agatccagac caagatactg aatcctggca ggcgcggcat tttcaggcga
    51361 gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt
    51421 gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc
    51481 ccggctgaac acgcggagcc agcaagcgca tagcctgttc catagtttcg gtttgttctg
    51541 ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg accgaaacaa tacagacgta
    51601 cgttatcgcc attcaggtaa cgcgccagtg ggctaaagtc cgccgattta ccatcgccgc
    51661 ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagcgccgct tccgtactgc
    51721 cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc gttatgctcc agcaccactt
    51781 caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggta
    51841 accctgcggc atctgccagc gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg
    51901 aaagtttcat ctctttcaca ttcagcactt tctcgccttt aacccgcagc caggtttcgc
    51961 cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat
    52021 ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa actttcgcgt
    52081 tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacggg tagcgatcca
    52141 tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaagctg gaggtggttt
    52201 ccagctggaa gctcgacagt tccagcacgt acagttcaca ctcatcatcc agtagcatca
    52261 acgcaggcag gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt
    52321 cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg
    52381 cttgtgcttc gcgacaaaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg
    52441 cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatctgccg
    52501 ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat
    52561 ccaggctagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga
    52621 aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca tttttacctt
    52681 ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga ccagcatcag
    52741 cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg
    52801 gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg
    52861 caggatgaca gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc
    52921 ctgacgtagc agtacggcga taatgcctaa cgcaccacct aacgccagcg aaccgacatc
    52981 gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac
    53041 tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc agatacggta tgtgtaaata
    53101 gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa
    53161 tacggtcggc ataattgcca gaccgtcaag accatcggtc aggtttaccg cgttgccagt
    53221 acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg gcatcacatc
    53281 tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacaaagc
    53341 gaaagcaaca cccagcgcaa tgaccgacat ccagaaatat ttccaacgag cgatcaaccc
    53401 tttggtatct ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc
    53461 taccagcacc accaacacgc accagacgta cggattggac gggtaagccc acagcagcac
    53521 ggagatcaca atcgctgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct
    53581 gaagtgtgat tcaggaccgt cgttacgcac tacctgacca aaggaaagtt tttgcaaatg
    53641 agcaatcatg cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat
    53701 ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc
    53761 ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggca
    53821 gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta
    53881 agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg
    53941 atagcatggc tttgtttacc catgcttaac acgcggtcaa taccagcagc ttttgccgcc
    54001 tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc
    54061 accagcacgc ggtagcccgg catttcagcc agtacctgaa cagctgcggt cattgaaccg
    54121 acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac
    54181 agacggccag gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg
    54241 cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc
    54301 aacggcagca gaacatcgac gctaccggtt ggggtttgta gggtaaattc cataccgtgc
    54361 gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc
    54421 cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc
    54481 ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca
    54541 cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgccgct
    54601 tccgggcgag tcagactcac agtccaggca atctcgccct gatggttcgc gccaagttca
    54661 ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac accgatgtcg
    54721 ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc
    54781 atctctttaa cggaggtttt gccggaggac cccgtcagag caaccacgcg cgccggaact
    54841 tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac
    54901 tgcggcaggt cgatgtccag cggacggcta accagtagtg cgcctgcgcc gccagctttc
    54961 gcctggtcgg caaaatcgtg ggcatcaaaa cgttcacctt tcagggcaac aaacaggcag
    55021 cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc
    55081 agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat gcaatccccc
    55141 ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct
    55201 ggtaatcctc atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa
    55261 cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca
    55321 ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg
    55381 tcaccaccgc cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt
    55441 tatcgcgatc gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg
    55501 ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg
    55561 gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg
    55621 ttttcagcag atcagccagt ggatagccca gcgccagcag tgttgccagc gcgagcaaca
    55681 ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccagcttg
    55741 aactaaagcg aatggtcgcg ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc
    55801 gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt
    55861 ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat
    55921 gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca tgataatcaa
    55981 ggtgatcgcg gcttaagttg gtaaagaccg acgccggaaa tttcaatgcc gccacacggt
    56041 gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca
    56101 gccccgccag ctcatgctga acatcaactg ccgaaccggt agtattttct gtcgggatca
    56161 ctttccccag cagaccatta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt
    56221 ggctccactg cgccagaagc tgggtcgtcg tggttttgcc gttggtgccc gttacgccta
    56281 cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct
    56341 cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg
    56401 tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga
    56461 tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag
    56521 ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg
    56581 gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca
    56641 aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt acgcaatacg
    56701 ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc
    56761 tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct
    56821 gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta
    56881 ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc gccgcctggt
    56941 agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg gaagacacgt
    57001 tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg
    57061 ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa
    57121 gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt
    57181 tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact
    57241 aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt
    57301 aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta
    57361 attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg
    57421 gtcatcacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg
    57481 ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta
    57541 gccatcgcca gcacttcacc tgtgttgaca tccaccagca cggcgctacc agattcagcc
    57601 ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag gcgttcatca
    57661 atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg
    57721 cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg
    57781 aaactcttct caacgccctc aatcccttga ctatcgacgt tagtaaagcc gatgaggtga
    57841 gcagtcactt cgccggacgg atagtaacgg cgagactctt cacgcagatg aatccccggc
    57901 agtttcagtt ttttgatgta gtccgccatg tcagagttta cctgacgcgc cagataaata
    57961 aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg aatattgagc
    58021 gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct
    58081 ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg
    58141 tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc
    58201 tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa
    58261 gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca
    58321 tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat
    58381 ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt
    58441 tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg
    58501 ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc
    58561 agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg
    58621 aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat
    58681 gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg
    58741 ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct
    58801 cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga cggccaccca
    58861 gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt
    58921 tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa
    58981 gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca
    59041 gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact
    59101 tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg
    59161 gctgttcgcg gttacgctcg acaatagcgc gggcaatgcg tttggcaaaa cgctcttcac
    59221 cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg
    59281 ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa
    59341 agccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt
    59401 cgatcttgcc aataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt
    59461 ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc
    59521 gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac
    59581 caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa
    59641 cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag
    59701 tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg
    59761 acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc
    59821 atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct
    59881 gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca
    59941 accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg
    60001 gggtaaagca gcaggcacgg gtgatgaatg tcaatggcgc aaaccatttg accggcagcg
    60061 ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt gctgtcgaga
    60121 ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac
    60181 aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg
    60241 cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc
    60301 aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca
    60361 ctgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga
    60421 gcggcatttt aggacatatc ttccccggtt aacagtcctt gttacgtctg tgtggcgctt
    60481 gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc aattcatcgc
    60541 cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc
    60601 agctggtgag ttaattaacg ggcgggttta aaaccattta tcaataatag tcaggaatag
    60661 tcttatttac tttaaggata ttgat
//
LOCUS       DADLXX010000009        59685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLXX010000009 DADLXX010000000
VERSION     DADLXX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.009"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(409..1575)
                     /locus_tag="JNX27_000542"
     CDS             complement(409..1575)
                     /locus_tag="JNX27_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HAY7881868.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(1597..2136)
                     /locus_tag="JNX27_000543"
     CDS             complement(1597..2136)
                     /locus_tag="JNX27_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881869.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(2392..2736)
                     /gene="tdcR"
                     /locus_tag="JNX27_000544"
     CDS             complement(2392..2736)
                     /gene="tdcR"
                     /locus_tag="JNX27_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7881870.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            2925..3863
                     /gene="tdcA"
                     /locus_tag="JNX27_000545"
     CDS             2925..3863
                     /gene="tdcA"
                     /locus_tag="JNX27_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7881871.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            3962..4951
                     /gene="tdcB"
                     /locus_tag="JNX27_000546"
     CDS             3962..4951
                     /gene="tdcB"
                     /locus_tag="JNX27_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7881872.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            4973..6304
                     /gene="tdcC"
                     /locus_tag="JNX27_000547"
     CDS             4973..6304
                     /gene="tdcC"
                     /locus_tag="JNX27_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7881873.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            6330..7538
                     /gene="tdcD"
                     /locus_tag="JNX27_000548"
     CDS             6330..7538
                     /gene="tdcD"
                     /locus_tag="JNX27_000548"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7881874.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            7572..9865
                     /gene="tdcE"
                     /locus_tag="JNX27_000549"
                     /pseudo
     CDS             7572..9865
                     /gene="tdcE"
                     /locus_tag="JNX27_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            9879..10268
                     /locus_tag="JNX27_000550"
     CDS             9879..10268
                     /locus_tag="JNX27_000550"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7881875.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            10340..11704
                     /gene="tdcG"
                     /locus_tag="JNX27_000551"
     CDS             10340..11704
                     /gene="tdcG"
                     /locus_tag="JNX27_000551"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7881876.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCD"
     gene            11979..13256
                     /locus_tag="JNX27_000552"
     CDS             11979..13256
                     /locus_tag="JNX27_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881877.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YSVSFTLAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILR
                     RKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLV
                     YKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            13284..14576
                     /locus_tag="JNX27_000553"
     CDS             13284..14576
                     /locus_tag="JNX27_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7881878.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14788..14952)
                     /locus_tag="JNX27_000554"
     CDS             complement(14788..14952)
                     /locus_tag="JNX27_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881879.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(14975..15676)
                     /locus_tag="JNX27_000555"
     CDS             complement(14975..15676)
                     /locus_tag="JNX27_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7881880.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            15781..16677
                     /locus_tag="JNX27_000556"
     CDS             15781..16677
                     /locus_tag="JNX27_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7881881.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            complement(16884..17252)
                     /locus_tag="JNX27_000557"
     CDS             complement(16884..17252)
                     /locus_tag="JNX27_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7881882.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(17545..18531)
                     /locus_tag="JNX27_000558"
     CDS             complement(17545..18531)
                     /locus_tag="JNX27_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7881883.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(18601..19083)
                     /locus_tag="JNX27_000559"
     CDS             complement(18601..19083)
                     /locus_tag="JNX27_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7881884.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFSFLTRTTT
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            complement(19179..19478)
                     /locus_tag="JNX27_000560"
     CDS             complement(19179..19478)
                     /locus_tag="JNX27_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881885.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(19468..19872)
                     /locus_tag="JNX27_000561"
     CDS             complement(19468..19872)
                     /locus_tag="JNX27_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7881886.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(19875..20180)
                     /locus_tag="JNX27_000562"
     CDS             complement(19875..20180)
                     /locus_tag="JNX27_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7881887.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(20218..20572)
                     /locus_tag="JNX27_000563"
                     /pseudo
     CDS             complement(20218..20572)
                     /locus_tag="JNX27_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            complement(20719..21102)
                     /gene="mzrA"
                     /locus_tag="JNX27_000564"
     CDS             complement(20719..21102)
                     /gene="mzrA"
                     /locus_tag="JNX27_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7881888.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(21106..21768)
                     /locus_tag="JNX27_000565"
     CDS             complement(21106..21768)
                     /locus_tag="JNX27_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7881889.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(22113..22889)
                     /gene="exuR"
                     /locus_tag="JNX27_000566"
     CDS             complement(22113..22889)
                     /gene="exuR"
                     /locus_tag="JNX27_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7881890.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            23185..23901
                     /locus_tag="JNX27_000567"
     CDS             23185..23901
                     /locus_tag="JNX27_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7881891.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            23931..24431
                     /locus_tag="JNX27_000568"
     CDS             23931..24431
                     /locus_tag="JNX27_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7881892.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            24523..27032
                     /locus_tag="JNX27_000569"
                     /pseudo
     CDS             24523..27032
                     /locus_tag="JNX27_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            27485..28117
                     /locus_tag="JNX27_000570"
     CDS             27485..28117
                     /locus_tag="JNX27_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7881893.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(28205..29503)
                     /locus_tag="JNX27_000571"
     CDS             complement(28205..29503)
                     /locus_tag="JNX27_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7881894.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            29986..31398
                     /gene="uxaC"
                     /locus_tag="JNX27_000572"
     CDS             29986..31398
                     /gene="uxaC"
                     /locus_tag="JNX27_000572"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7881895.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSSRGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            31413..32900
                     /locus_tag="JNX27_000573"
     CDS             31413..32900
                     /locus_tag="JNX27_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY7881896.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            32983..33534
                     /locus_tag="JNX27_000574"
     CDS             32983..33534
                     /locus_tag="JNX27_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7881897.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(33539..34783)
                     /gene="sstT"
                     /locus_tag="JNX27_000575"
     CDS             complement(33539..34783)
                     /gene="sstT"
                     /locus_tag="JNX27_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7881898.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(35379..36344)
                     /gene="alx"
                     /locus_tag="JNX27_000576"
     CDS             complement(35379..36344)
                     /gene="alx"
                     /locus_tag="JNX27_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7881899.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(36615..37631)
                     /locus_tag="JNX27_000577"
     CDS             complement(36615..37631)
                     /locus_tag="JNX27_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7881900.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(37692..38384)
                     /locus_tag="JNX27_000578"
     CDS             complement(37692..38384)
                     /locus_tag="JNX27_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7881901.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(38461..38964)
                     /locus_tag="JNX27_000579"
     CDS             complement(38461..38964)
                     /locus_tag="JNX27_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7881902.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            39049..40185
                     /gene="rlmG"
                     /locus_tag="JNX27_000580"
     CDS             39049..40185
                     /gene="rlmG"
                     /locus_tag="JNX27_000580"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7881903.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            40470..40784
                     /locus_tag="JNX27_000581"
     CDS             40470..40784
                     /locus_tag="JNX27_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HAY7881904.1"
                     /translation="MHLITQKALKDAAEKHPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            40781..41197
                     /gene="higA"
                     /locus_tag="JNX27_000582"
     CDS             40781..41197
                     /gene="higA"
                     /locus_tag="JNX27_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7881905.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(41242..43260)
                     /locus_tag="JNX27_000583"
     CDS             complement(41242..43260)
                     /locus_tag="JNX27_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7881906.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLQLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(43486..45837)
                     /gene="ygjK"
                     /locus_tag="JNX27_000584"
     CDS             complement(43486..45837)
                     /gene="ygjK"
                     /locus_tag="JNX27_000584"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY7881907.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(45854..46925)
                     /gene="ygjJ"
                     /locus_tag="JNX27_000585"
                     /pseudo
     CDS             complement(45854..46925)
                     /gene="ygjJ"
                     /locus_tag="JNX27_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(47059..48492)
                     /locus_tag="JNX27_000586"
     CDS             complement(47059..48492)
                     /locus_tag="JNX27_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7881908.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(48555..49004)
                     /locus_tag="JNX27_000587"
     CDS             complement(48555..49004)
                     /locus_tag="JNX27_000587"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7881909.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(49001..52087)
                     /gene="ebgA"
                     /locus_tag="JNX27_000588"
     CDS             complement(49001..52087)
                     /gene="ebgA"
                     /locus_tag="JNX27_000588"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7881910.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAYYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIINRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(52269..53252)
                     /gene="ebgR"
                     /locus_tag="JNX27_000589"
     CDS             complement(52269..53252)
                     /gene="ebgR"
                     /locus_tag="JNX27_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7881911.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELNN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            53471..53802
                     /locus_tag="JNX27_000590"
                     /pseudo
     CDS             53471..53802
                     /locus_tag="JNX27_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(53844..55286)
                     /gene="ygjG"
                     /locus_tag="JNX27_000591"
     CDS             complement(53844..55286)
                     /gene="ygjG"
                     /locus_tag="JNX27_000591"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7881912.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLDGFR
                     QLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEP
                     PLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            55592..57112
                     /locus_tag="JNX27_000592"
     CDS             55592..57112
                     /locus_tag="JNX27_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7881913.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMFTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(57266..57889)
                     /locus_tag="JNX27_000593"
     CDS             complement(57266..57889)
                     /locus_tag="JNX27_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7881914.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            58166..58912
                     /gene="nfeF"
                     /locus_tag="JNX27_000594"
                     /pseudo
     CDS             58166..58912
                     /gene="nfeF"
                     /locus_tag="JNX27_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(58966..59041)
                     /locus_tag="JNX27_000595"
     tRNA            complement(58966..59041)
                     /locus_tag="JNX27_000595"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59006..59008),aa:Ile,seq:cat)
     gene            59166..59672
                     /gene="mug"
                     /locus_tag="JNX27_000596"
     CDS             59166..59672
                     /gene="mug"
                     /locus_tag="JNX27_000596"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY7881915.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT        14814 a        14597 c        15312 g        14962 t
ORIGIN      
        1 ggtcagtttc acctgatata cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg
       61 cagaaagatg aatgactgtc cacgacacta tacccaaaag aaagcggctt atcggtcagt
      121 ttcacctgat gtacgtaata aaccgttccg gcgggtttca gattgttgag tgcgctttat
      181 tcatgccgga tgctgcgtga acgccttatc ctgcctacaa aagcacgcaa attcaatata
      241 ttgcagagat ggcgtaggcc tgataagcgt agcgcatcag gcatttttgc ggttgccatc
      301 agtttcaaac ccgcgtcgat aagtttttgc ttttggtgca cagggtaaga tgaatatcta
      361 cggtattaat aatagacttt gtaagaagtt attaagtcta tattgcattt aaaaggtcgt
      421 aattatacgt atagtcctct ctggtaaaat attgattctc tgagttatat gcttcatatg
      481 tgttatattc cccgacctca aagccattgt ccgtagaatt ttcgtctttc tcgagggtat
      541 taactcttct gagctagagt cttctatgtt taaatccata gtataattaa tttgggggac
      601 gaccagataa gcattttctt tgatattttc ataatcttca atgattgtat cgatgtaatc
      661 atatattttt tttgatgaga attctttcat ctctggtaat ttaattctgt tcaagcaatt
      721 gttgattttc gatgagcttt tatatatttc ttttgttaat tcattttcta atttatctgt
      781 aagagttgcc cttggtatag attttataat tgcaaatctc ataattttta attgttcttc
      841 ggtaatgtct ccaagctcat ttaaacaact ttcgacaaac ttgatttttt cttcagaata
      901 catatcgcta atgcttatta aaacaggcgt tatttttagt aaataatccc aggagattgt
      961 ggcgttttgt gttgaactaa caaaattaac ctgaaacatg ttggcattag taaaatttgt
     1021 tgaatccaat ttaaaatgat ttagaatagt attagccata tgtttatctg tgaaatcata
     1081 ataatctaaa tcgacattac gtaagtccag agaggataga tcaactatat ctccagtccc
     1141 acaactaaag attacgttca acctcctgat ttcattgtaa atatctttta atgggcaatt
     1201 aacattgata ttgcctgtag atatttcctg gttttttaac ggttcattaa tggtaagttc
     1261 gcaggtattg tccgatcgtt gaataatttg ggcgttataa cctaaaaaat ccagacgaag
     1321 agacgagtca ttataaagaa ttacattttg taagtacgct aattcttttt gaaaatcacc
     1381 agtgataatg cattttccac ttggttgtaa cgcgttaagt aaagtgacca tttgaataga
     1441 ggtctgtttt agtttttcta taccgggtgc tagtgcttcg aggttatttc ctatggggct
     1501 ttcaggaagg tcattcattt ttcgacggac taaatcacta ctaatatcat taccaattga
     1561 tgaaacaggg aacattattt tatcctttat aaataatcaa taaaaatcat tattaaaatg
     1621 atcgggtgaa aaaaagagaa tttgccaaca atctgaagta atgcaggttt tattaaagtg
     1681 cctagggggt gttaagatgg cttctttttg ggtgaacttt gcgcaatact tagaaatcgt
     1741 ctccttaata cattccagct cttttttatt gacggctgag caagtacatt gttcaccaaa
     1801 aggtgcaaaa gtgcctacat ctaatgtttg tatgatataa tcatcatgtt ctcctgtaga
     1861 aatatctatg atgaatggac tcatattttc cggttggatt cggatcatga cagaaggaac
     1921 atcgccttga tagaaattca atgcagtaat tgcagataat actctccgca gttcattact
     1981 tatactgtaa attttattaa cgtcattggt ggagacaatt tctgcaaact ttctttttac
     2041 cgtccaataa tcaatatttc tattgtgatt gttaaccact gcgtgcattg gcgggattga
     2101 agggtgaaaa atatgtgcaa ttgggaagcc tttcattgat gcctgccttg tcgtcatgac
     2161 aaacaataat agatgcttat tttcctcgca cgacacttac gcactatcaa aaaacgctca
     2221 tttacccggc gttacagtaa acgaagcaac attctgtgtc ctacgctgat gtaaagcaca
     2281 gtgaaaatcc cccttgttga cttcgtctga ccttatcgcg gcgaccagca attattcgtt
     2341 ttgtgatcct caacacaaaa tgctattcgt cgcctatgag gggagaagaa tttatggaaa
     2401 acgattgcat tcacaaaatg acgtcctgtc agaagagaaa aagttgacgt taatcaattg
     2461 ttcgagatga tcaatacctg tattcgaaat tttaaaatat tgataaaata aatgtttgtt
     2521 ctccaacaaa tagcgtgaaa aaatgtgacg tgccgcagta tttatcgctg aaaatggttt
     2581 atttataata aaatttgaga acttgctcat taaatttaac tcaaattttg cctggtaatt
     2641 atccggtaat tgcttgaaat atggtctcag cccctttttt gtattaacca cataacgaat
     2701 gatgttatcg ccataaaata tggttatccc cgtcattttt tttgatagaa atcagggttt
     2761 atgctgattt ttatgcttta acttgttgat atttaaaggt atttaattgt aataacgata
     2821 ccttggaaag taatgaaagt taatttgtga gtggtcgcac atatcctgtt tatttcattt
     2881 tgatacactt catgccgtca atgaggtaat taacgtaggt cgttatgagc actattcttc
     2941 ttccgaaaac gcagcacctg gtagtctttc aggaagtcat tagaagtggc tctatcggct
     3001 cggctgcaaa agaattaggg ttaactcaac cggccgtcag taaaatcatt aacgatattg
     3061 aagattattt tggtgtggaa ttagtggtgc ggaaaaatac cggtgtaaca ttaacacctg
     3121 ccggtcaatt gttactctcc cgttccgaat ccattacccg tgaaatgaaa aatatggtta
     3181 atgagataag cggtatgtct tctgaggcgg tggtggaagt ctcatttggt tttccttcat
     3241 tgattggttt tacttttatg tcagggatga tcaacaagtt caaagaggtg ttcccgaaag
     3301 cgcaggtttc tatgtatgaa gcgcaactgt cttcgttctt accggcaatc cgcgacggtc
     3361 gactggattt tgcgattggt acgttaagtg cagaaatgaa gcttcaggat ttacatgttg
     3421 agccgctgtt cgagtccgag tttgtgctgg tagccagtaa gtcccgaaca tgcaccggca
     3481 ccaccacgct ggagtcgttg aagaacgaac agtgggtgtt gccacaaact aatatggggt
     3541 actacagcga actgcttact acgttacaaa gaaatggcat cagtattgaa aacatcgtta
     3601 aaaccgactc agtcgtgaca atttataatc ttgttctcaa tgctgatttc ttaactgtaa
     3661 ttccttgtga tatgacgtca ccttttggtt ctaatcaatt tattactatt ccggttgaag
     3721 aaacattacc tgtggcacaa tatgccgcgg tatggtcgaa aaattatcgt attaaaaaag
     3781 cagcatcggt tttggtggaa ttagccaaag agtattcatc ttataatggg tgtagacgaa
     3841 ggcaattaat agaagttggt tagttatttg tttttattta aacataaata atccacctgt
     3901 ctgttttgca ggtgtcggtt acggttacct acatatttaa ttcaggcgaa gaggttttat
     3961 aatgcatatt acatacgatc tgccggttgc tattgatgat attattgaag cgaaacaacg
     4021 actggctggg cgaatttata aaacaggcat gcctcgctcc aactatttta gtgaacgttg
     4081 caaaggtgaa atattcctga agtttgaaaa tatgcagcgt acgggttcat ttaaaattcg
     4141 tggcgcattt aataaattaa gttcactgac cgatgcggaa aaacgcaaag gcgtggtggc
     4201 ctgttctgcg ggcaaccatg cgcaaggggt ttcgctctcc tgcgcgatgc tgggtatcga
     4261 cggtaaagtg gtgatgccaa aaggtgcgcc aaaatccaaa gtagcggcaa cgtgcgacta
     4321 ctccgcagaa gtcgttctgc atggtgataa cttcaacgac actatcgcta aagtgagcga
     4381 aattgtcgaa atggaaggtc gtatttttat cccaccttac gatgatccga aagtgattgc
     4441 tggccaggga acgattggtc tggaaattat ggaagatctc tatgatgtcg ataacgtgat
     4501 tgtgccaatt ggtggtggtg gtttaattgc tggtattgcg gtggcaatta aatctattaa
     4561 cccgaccatt cgtgttattg gcgtacagtc agaaaacgtt cacggcatgg cggcttcttt
     4621 ccactccgga gaaataacca cgcaccgaac taccggcacc ctggcggatg gttgtgatgt
     4681 ctcccgcccg ggtaatttaa cttacgaaat cgttcgtgaa ttagtcgatg acatcgtgct
     4741 ggtcagcgaa gacgaaatca gaaacagtat gattgcctta attcagcgca ataaagtcgt
     4801 caccgaaggc gcaggcgctc tggcatgtgc tgcattatta agcggtaaat tagaccaata
     4861 tattcaaaac agaaaaaccg tcagtattat ttccggcggc aatatcgatc tttctcgcgt
     4921 ctctcaaatc accggttttg ttgacgctta attaattcgt tgaggatagg atatgagtac
     4981 ttcagatagc attgtatcca gccagacgaa acaatcgtcc tggcgtaaat cagataccac
     5041 atggacgtta ggcttgtttg gtacggcaat cggcgccggg gtgctgttct tccctatccg
     5101 cgcaggtttt ggcggactga tcccgattct tctgatgttg gtattggcat accccatcgc
     5161 attttattgc caccgggcgc tggcgcgtct gtgtctttct ggctctaacc cttccggcaa
     5221 cattacggaa acggtggaag agcattttgg taaaactggc ggcgtggtta tcacgttcct
     5281 gtacttcttc gcgatttgcc cactgctgtg gatttatggc gttactatta ccaatacctt
     5341 tatgacgttc tgggaaaacc agctcggatt tgcaccgctg aatcgcggct ttgtggcgct
     5401 gttcctgttg ctgctgatgg ctttcgtcat ctggtttggt aaggatctga tggttaaagt
     5461 gatgagctac ctggtatggc cgtttatcgc cagcctggtg ctgatttctc tgtcgctgat
     5521 cccttactgg aactctgcag ttatcgacca ggttgacctc ggttcgctgt cgttaaccgg
     5581 tcatgacggt atcctgatca ctgtctggct ggggatttcc atcatggttt tctcctttaa
     5641 cttctcgcca atcgtctctt ccttcgtggt ttctaaacgt gaagagtatg agaaagactt
     5701 cggtcgcgac ttcaccgaac gtaaatgttc ccaaatcatt tctcgtgcca gcatgctgat
     5761 ggttgcagtg gtgatgttct ttgcctttag ctgcctgttt actctgtctc cggccaacat
     5821 ggcggaagcc aaagtgcaga atattccagt gctttcttat ctggctaacc actttgcgtc
     5881 catgaccggt acgaaaacaa cgttcgcgat tacactggaa tatgcggctt ccatcatcgc
     5941 actcgtggct atcttcaaat ctttcttcgg tcactatctg gggacgctgg aaggcttgaa
     6001 tggtctgatt ctgaagttcg gttataaagg tgacaaaacc aaagtgtcgc tgggtaaact
     6061 gaatactctc agcatgatct tcatcatggg ctccacctgg gttgttgcct acgccaaccc
     6121 gaacatcctc gacctgattg aagccatggg cgcaccgatt atcgcatccc tgctgtgcct
     6181 gttgccgatg tatgctatcc gtaaagcgcc gtctctggcg aaataccgtg gtcgtctgga
     6241 taacgtgttt gttaccgtga ttggtctgct gaccatcctg aacatcgtat acaaactgtt
     6301 ttaatccgta actcaggatg agaaaagaga tgaatgaatt tccggttgtt ttggttatta
     6361 actgtggttc gtcttcgatt aagttttccg tactcgatgc cagcgactgt gaagtattaa
     6421 tgtcaggtat tgccgacggt attaactcgg aaaatgcatt cttatccgta aatgggggag
     6481 agccagcacc gctggctctc cacagctacg aaggtgcatt gaaggcaatt gcatttgaac
     6541 tggaaaaacg gaatttaaat gacagcgtgg ccttaattgg ccaccgcatc gctcacggcg
     6601 gcagtatttt taccgagtcc gccattatta ccgatgaagt cattgataat atccgtcgcg
     6661 tttctccact ggcacccctg cataattacg caaatttaag tggtattgaa tcggcgcagc
     6721 aattatttcc gggcgtaact caggtggccg tatttgatac cagtttccac cagacgatgg
     6781 ctccggaagc ttatttatac ggcctgccgt ggaaatatta tgaagagtta ggtgtacgcc
     6841 gttatggttt ccacggcacg tcgcaccgct atgtttccca gcgcgcacat tcgctgctga
     6901 atctggcgga agatgactcc ggcctggttg tggcgcatct tggcaatggc gcgtcaatct
     6961 gcgcagttcg caacggtcag agtgttgaca cctcaatggg aatgacgccg ctggaaggct
     7021 tgatgatggg tacccgcagt ggcgatgtcg actttggtgc gatgtcctgg atcgccagcc
     7081 aaaccaacca gagcctgggt gacctggaac gcgtagtgaa taaagagtcg ggattattag
     7141 gtatttccgg tctttcttcg gatttacgtg ttctggaaaa agcctggcat gaaggtcacg
     7201 aacgcgcgca actggcaatt aaaacctttg ttcaccgaat tgcccgtcat attgccggac
     7261 atgcagcttc attacgtcgg ctggatggaa ttatattcac cggcggaata ggagagaatt
     7321 caagtttaat tcgtcgtctg gtcatggaac atttggctgt attaggcgta gagattgata
     7381 cagaaatgaa taatcgatct aactcttttg gcgagcgaat tgtttccagt gaaaatgcgc
     7441 atgtcatttg cgtcgttatt ccgactaacg aagaaaaaat gattgctttg gatgccattc
     7501 atttaggtaa agttaacgcg cccgcagaat ttgcataatt tagttgaagt attgtagaga
     7561 gattattttt catgaaggta gatattgata ccagcgataa gctgtacgct gacgcatggc
     7621 ttggctttaa aggtacggac tggaaaagcg aaattaatgt ccgcgatttt attcaacata
     7681 actatacacc gtatgaaggc gatgaatctt ttctcgccga agcgacgcct gccaccacgg
     7741 aattgtggga aaaagtaatg gaaggcatcc gtatcgaaaa cgcaacccac gcgccggttg
     7801 atttcgatac caatattgcc accacaatta ccgctcatga tgcgggatat attaaccagc
     7861 cgctggaaaa aattgttggc ctgcaaacgg atgcgccgtt gaaacgtgcg ctacacccgt
     7921 tcggtggcat taatatgatt aaaagttcat tccacgccta tggccgtgaa atggacagtg
     7981 aatttgaata tctgtttacc gatctgcgta aaacccataa ccagggcgta tttgatgttt
     8041 actcaccgga tatgctgcgc tgccgtaaat ctggcgtgct gaccggttta ccagatggct
     8101 atggtcgtgg gcgcattatc ggtgactatc gccgcgtagc gctgtatggc atcagttatc
     8161 tggtgcgtga acgcgaactg caatttgccg atctccagtc tcgtctggaa aaaggcgagg
     8221 atctggaagc caccatccgt ctgcgtgagg agctggcaga gcatcgtcat gcgctgttgc
     8281 agattcagga aatggcggcg aaatatggct ttgatatctc tcgcccggcg cagaatgcac
     8341 aggaagcggt gcagtggctc tacttcgctt atctggcggc agtgaaatcg caaaatggcg
     8401 gcgcgatgtc gctggggcgc acggcatcgt tcctggatat ctacattgag cgcgacttta
     8461 aagctggcgt cctcaatgag cagcaggcac aggaactgat cgatcacttc atcatgaaga
     8521 tccgtatggt acgcttcctg cgtacaccgg aatttgattc gctgttctcc ggcgacccaa
     8581 tctgggcgac ggaagtgatc ggcgggatgg ggctggacgg tcgtacgctg gtgaccaaaa
     8641 actccttccg ctatttgcac acctgcacac tatggggccg gcaccggaac ctaacctgac
     8701 cattctttgg tcggaagaat taccgattgc cttcaaaaaa tatgccgcgc aggtgtcgat
     8761 cgtcacctct tccttgcagt atgaaaatga cgatctgatg cgtactgact tcaacagcga
     8821 cgattacgcg attgcctgct gcgtcagccc aatggtgatt ggtaagcaaa tgcagttctt
     8881 tggtgcacgc gctaacctgg cgaaaacgct gctctacgca attaacggcg gggtggacga
     8941 gaagctgaag attcaggtcg ggccgaaaac agcaccgctg atggacgacg tgctggatta
     9001 cgacaaagtg atggacagcc tcgatcactt catggactgg ctggcggtgc agtacatcag
     9061 cgcgctgaat atcattcact acatgcacga caagtacagc tacgaagctt cgctgatggc
     9121 gctgcacgat cgtgatgtct atcgcactat ggcatgcggc atcgcgggcc tgtcggtggc
     9181 gacggactcc ctgtctgcca tcaaatatgc ccgcgtgaaa ccaatccgtg acgaaaacgg
     9241 cctggcggtg gactttgaaa tcgacggtga atatccgcag tacggcaaca acgacgagcg
     9301 cgtagacagc attgcctgcg acctggttga acgctttatg aagaaaatta aagcgctgcc
     9361 aacctatcgc aacgccgtcc ctacccagtc gattctgact atcacttcta acgtggtgta
     9421 cggccagaaa accggtaata cgccggacgg tcgtcgcgcc ggaacaccgt tcgcgccggg
     9481 cgctaacccg atgcatggtc gtgaccgcaa aggtgccgtg gcctcattga cgtcggtggc
     9541 gaaactgccg ttcacctacg ccaaagatgg gatctcgtac accttctcaa tcgttcctgc
     9601 ggcgctgggc aaagaagatc cagtacgtaa aaccaacctt gtcggcctgc tggatgggta
     9661 tttccaccac gaagcggatg tcgaaggcgg tcaacacctc aacgtcaacg taatgaatcg
     9721 ggaaatgctg ctggatgcca tcgagcaccc ggaaaaatat cctaacctga caatccgtgt
     9781 ctctggctac gccgtgcgct tcaacgcact gacccgtgaa cagcaacagg atgttatttc
     9841 acgtactttt acccaggcgc tctgacgccg gaggatgtat gaaaaagatt atcgaaacgc
     9901 aacgtgccct aggcgcaatc ggcccttatg ttcagggcgt tgatttaggc agcatggtct
     9961 tcacctccgg gcaaattccg gtttgcccgc agaccggtga gatcccggct gatgtgcaag
    10021 atcaggcgcg tttaagcctc gaaaacgtca aagcgatcgt ggttgctgcc gggctgagcg
    10081 tgggcgatat catcaagatg accgtgttca tcaccgatct gaatgatttt gccaccatca
    10141 atgaagtcta taagcagttc ttcgatgagc atcaggcgac ctacccgacc cggagctgtg
    10201 tgcaggttgc gcgtttgccg aaagatgtga agctggaaat tgaagctatc gcagtacgta
    10261 gtgcgtaaag cctcgtgagc gggacggtcg caaggtcgtt ccgctccact tcactgaacg
    10321 gcaatccgag ggtgtggata tgattagtgc attcgatatt ttcaaaattg ggattggtcc
    10381 ctccagttcg cataccgtgg ggccaatgaa tgccggaaaa agttttattg atcggctgga
    10441 aagtagcggc ttattaaccg cgacgagcca tattgtggtc gatctgtacg ggtcgttgtc
    10501 actgacgggc aaaggccatg ccacggatgt cgccattatc atgggactgg caggaaacag
    10561 tccgcaggat gttgtcattg atgagatccc tgcatttata gagttagtaa cgcgcagcgg
    10621 gcggctgcca gtggcatctg gtgcgcatat tgttgatttt cctgtagcaa agaacattat
    10681 cttccatccc gaaatgttgc ctcgccatga gaacggaatg cggatcactg cctggaaggg
    10741 acaggaagcg ctattaagta aaacctatta ctctgtcggc ggcgggttta ttgtcgaaga
    10801 agaacacttc ggcctgtcgc acgatgtcga aacgtccgta ccttacgatt tccactcagc
    10861 aggtgaactg ctgaaaatgt gtgattacaa cggcctgtct atatctggcc tgatgatgca
    10921 caacgagctg gcgctgcgca gcaaagcgga aattgacgca gggtttgccc gtatctggca
    10981 agtgatgcat gacggtattg aacgcgggat gaacactgaa ggcgtgctgc ctggtccgct
    11041 caatgtgccg cgtcgtgccg tagcgctgcg tcgtcagctg gtttctagcg ataacatctc
    11101 taacgatccg atgaatgtca tcgactggat caacatgtac gcgctggcgg ttagtgaaga
    11161 aaacgcagct ggcgggcgcg tggtaacggc accgactaac ggtgcgtgcg gcattattcc
    11221 ggcagtgctg gcttattacg ataagttccg tcgtccggta aacgagcggt caattgcccg
    11281 ctattttctg gcagcggggg ctattggcgc gctgtataaa atgaacgcct ccatctctgg
    11341 cgcggaggtc ggctgtcagg gggagattgg cgtggcctgt tcaatggcag cggcagggtt
    11401 aactgaacta ctgggcggca gtccggcgca ggtatgcaat gcggcggaaa tcgcgatgga
    11461 gcataacctt gggctgacct gcgatccggt tgccggacag gtacaaatcc cgtgcattga
    11521 acgtaatgcc attaatgccg tgaaagcagt aaacgccgcg cggatggcga tgcgccgcac
    11581 ctcggcaccg cgtgtttcac tcgataaagt gatcgagacg atgtatgaaa ccggcaaaga
    11641 tatgaacgat aaataccgcg aaacatcacg cggaggactg gccattaaag tggtctgcga
    11701 ctgacattgc tgtcagcttt catccttggg tgcacaaatg tgcacctttt ttttgtgatc
    11761 tgccgcaaat agagaatgaa tattctcttg cgattaattt agtagaaaat tcttatccat
    11821 ctccgttatt ttaaagtctt gattgtgaag attttctctg acctcgcgcg tatcatttct
    11881 ttattctaat aactcgcctg caaaaacgta gaccgcatat cgccttgatg cggctcggaa
    11941 aaaaataata agccctacaa aatattgagc gagaaattat ggaaattgca tcgaataaag
    12001 gcgtcattgc agacgcttcg accccggcgg gtcgtgctgg aatgagtgag agcgagtggc
    12061 gagaagcgat caaattcgac agtactgaca ccggctgggt gattatgagt atcgggatgg
    12121 cgattggcgc ggggattgtt tttctcccgg tgcaggtcgg tttgatggga ttgtgggtat
    12181 ttttgctctc atcggtgatt ggttacccgg caatgtatct gtttcagcgg ttgtttatta
    12241 atacgctggc agaatcacca gaatgtaaag attacccgag tgtcattagc ggttatttag
    12301 gtaaaaactg gggcatcctg ttaggtgcgc tctatttcgt aatgctggtg atttggatgt
    12361 tcgtctattc caccgccatc accaacgata gtgcttccta tctgcatacc ttcggcgtga
    12421 cggaagggtt gctgtcagac agtccctttt atggtctggt actgatttgc attctggtgg
    12481 cgatctcttc acgcggcgag aaattgttat tcaaaatttc gaccgacatg gtgctgacca
    12541 agctgctggt agtcgctgcg ctgggcgtgt cgatggtggg aatgtggcat ctgtataacg
    12601 tcggttcgct accgccgctg gggctgctgg tgaaaaacgc cattattacg ctgccgttta
    12661 ccctgacgtc gattctgttt atccagacgt taagcccgat ggtgatctct tatcgctcgc
    12721 gggaaaaatc aattgaagtg gcgcggcata aagcattgcg ggcaatgaat atcgcgtttg
    12781 gcattttgtt tgtcaccgtc tttttctact ccgtgtcgtt cacgctggcg attgccgcac
    12841 agtttattag cggtgacggc gcagcgtggg tgaaagtggt cagcgtcatt ctcaatatct
    12901 ttgctgtcat gactgcattc tttggcgtct atttaggctt tcgcgaagca acgcaaggga
    12961 tcgtaatgaa catcctgcgt cgcaagatgc ctgccgagaa gattaacgaa aatcttgttc
    13021 agcgcggcat catgattttc gccattttgc tggcctggag cgccatcgta ctgaacgcac
    13081 cggtgttgag cttcacctct atctgtagcc cgattttcgg catggtaggg tgcctgatcc
    13141 cggcgtggct ggtttacaaa gtaccggcat tgcacaaata caaagggatg tctctgtacc
    13201 tgattatcgt cactggtttg ttgctttgtg tttctctgtt cctggcattt tcttgattta
    13261 cctgaaattt taaggttttt aatatgtttg attcgacttt aaatccgtta tggcagcgtt
    13321 acatcctcgc cgttcaggag gaagtaaaac cggcgctggg atgtactgaa ccgatttcac
    13381 tggcgctggc ggcggcggtt gctgcggcag aactggaagg tccggttgaa cgtgtagaag
    13441 cctgggtttc gccaaatctg atgaagaacg gtctgggcat caccgttccc ggcacgggaa
    13501 tggtggggct gccgattgcg gcggcgctgg gggcgttagg tggaaatgcc aacgccgggc
    13561 tggaagtgct gaaagacgca actgcgcagg caattgccga tgccaaagca ctgctggcgg
    13621 cggggaaagt ctctgttaag atccaggaac cttgcaatga aatcctcttc tcacgcgcca
    13681 aagtctggaa cggtgagaag tgggcgtgtg tcaccatcgt cggcgggcat accaacattg
    13741 tgcatattga gacgcacaat ggtgtggtgt ttacccagca ggcgtgtgtg gcagagggcg
    13801 agcaagagtc gccgctgacg gtgctttcca gaacgacgct ggctgagatc ctgaagttcg
    13861 tcaatgaagt cccatttgcg gcgatccgct ttattctcga ttccgcgaag ctaaattgtg
    13921 cgttatcgca ggaaggtttg agcggtaagt gggggctgca tattggcgcg acgctggaaa
    13981 aacagtgcga gcgcggtttg ctggcgaaag atctctcttc atccattgtg attcgtacca
    14041 gcgcggcatc cgatgcgcgt atgggcggcg ctacgcttcc ggctatgagt aactccggct
    14101 cgggtaacca ggggatcacc gcaacaatgc ctgtggtggt tgtagcagaa cacttcggag
    14161 cggatgatga acgactggcg cgtgcgctga tgctttctca tttgagcgca atttacatcc
    14221 ataaccagtt accgcgtttg tctgcacttt gtgccgcaac gaccgcagca atgggggccg
    14281 ccgccgggat ggcatggctg gtggatgggc gttatgaaac tatctcgatg gcgatcagca
    14341 gtatgatcgg cgatgtcagc ggcatgattt gcgatggtgc gaaggtttcg accagtgctt
    14401 cggctgcgtg gaaagcggtg ttaatggcgc tggatgatac cgccgtgacc ggcaatgaag
    14461 ggatcgtggc gcatgatgtt gagcagtcga ttgccaacct gtgtgcgtta gcaagccatt
    14521 cgatgcagca aacggatcgg cagattatcg agattatggc gagcaaggcc agataaacgc
    14581 caggttttga gtattgtgcc ggatgcgatg ctggcgcacc ttatccggcc tacggggtgc
    14641 tcaacttgtt gatgttgttg tgttttgtac ctgatgccgg atgcgatgct ggcgcatctt
    14701 atccggccta cggggtgttt aatttgttga tgttgttgtg ttttgtaggc cggataaggc
    14761 gtttacgccg catccggcaa cctgtcttta cgcggtagct tcatcctcgg ctaaacgcgt
    14821 ttcagcatcc gcgacgatag cttccagctc gctcaacatc tcttcatagc caaaattttt
    14881 ggcggtgcga gcaggttctt tcgccaccac gggcttcgct agctggcgct ttttggtcga
    14941 ttttttactc atcatcatct ccctgtaaaa acaactagac aggcaaatct atcagcaaag
    15001 cgcgcagtgg ggaatcggca accagcgtaa tgttagcctc gtcacgaata aacgccccat
    15061 caccgcaggt tagcgccgct ttctcttcat gatgcgtaag cgcatgaaat ttcccgtgaa
    15121 tcgattgcaa atacgcgcgt ggcccatgca actggaaatt cgcactttcg cctttgtcga
    15181 gcacgatatg gtgcagccac acttgttggc gtaattgcag gcttcccatc gcaccctctg
    15241 gcgaggcgat taattgctgc ttgtccatat taagcgccag cttttgaatc agcggattct
    15301 ctcgctgcgg gcaggtatcc agccaaagct gcattcgcgt taacggtttg tctttgctga
    15361 gattgtgttc gctatagctg acacccggct gggtagagag cagcaacgcc tctccggcgc
    15421 tggcctgaac atgattgcct tcgctgtcgc gatactctgc ttctccatcc agaatcacat
    15481 ttaaaatatc gactttggga taggttcgcg gctgaaaggc ggcacctggg gccagcactt
    15541 cctggttaag cacacgcagg gaggcatagc ctaacaattt cgggtcgaag tagtgtccaa
    15601 aggaaaaagt ataccgggcc tgcaaccatc cgtagtctgc ttgtccacac tgcctggcag
    15661 ttcgggtagt aatcataatt cttgacctct ctttacttcc ttttatggta aggggctgga
    15721 cgctacattg ttagccagat attctgcccg gtatgttcaa atttcctgaa tgagaacgaa
    15781 atggccaaag aaagggcatt aacgctggaa gcactacggg ttatggatgc gatcgatcgc
    15841 cggggcagtt ttgcggcggc agcggatgag ctgggacgcg tgccttccgc acttagctac
    15901 atcatgcaaa aactggaaga agagctggat gtggtgctgt ttgaccgctc gggccatcgc
    15961 accaaattca ccaatgtcgg gaggatgttg ctggagcgag ggcgcgtttt gctggaagcc
    16021 gcagataaac tgactactga tgcggaagct ctcgcgcgcg gttgggaaac gcatctcacc
    16081 attgtgaccg aagcgctggt accgacacct gcctttttcc cgttaatcga caaactggcg
    16141 gcaaaagcca atacccaact ggcaatcatc acagaagtgc tggcgggggc gtgggaacgg
    16201 ctggagcagg ggcgggcgga tatcgttatc gcgccggata tgcattttcg ttcctcgtcg
    16261 gagatcaact cgcgcaagct ctatacgtta atgaacgtct acgttgccgc gcctgatcac
    16321 ccgattcatc aggagccgga accgttatct gaagtgacgc gtgtgaaata tcgtggaatt
    16381 gcggtagcgg ataccgctcg tgagcgcccg gtgttgaccg tacagctgct ggacaaacag
    16441 ccgcgcttaa cggtgagcac gattgaagat aaacgtcagg cattactggc ggggcttggc
    16501 gtggcgacga tgccgtatcc gatggtcgaa aaagatattg cggaagggca gttgcgtgtc
    16561 gtcagcccgg aatcgaccag cgagatcgat attattatgg cctggcgtcg tgacagtatg
    16621 ggggaagcga aatcctggtg tctgcgggaa attccaaagt tgttcgctgg aaaataatct
    16681 attaagcacc tttcacaagg tgcttctgac tgctgacaaa cgcaaaactg cctgatgcgc
    16741 tacgcttatc aggcctacgc agtcactgca atatattgaa ttttggcaat ttgtaggctg
    16801 gataaggcgt ttacgccgca tccggcataa acaaagcgca ctttgtcaac aatttgaagc
    16861 accttttaca aggtgcttaa tcattattct tgttccagtt ttggatctgg tccaaagcgg
    16921 ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca gccgataaac
    16981 gggattaaga atagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag gcggcggaac
    17041 tgaaccgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag gatgccttcg
    17101 ccaccagcgc gttgccagcc taacatttta tccagcagcc ccagcacgaa cgtaaagatg
    17161 atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg gaaaccgaca
    17221 taatttttta gtactttcag ataccagtcc atttttgcct caattttgct caatcacttg
    17281 tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata ttcgttcaag
    17341 gaattaataa agtgccgtga aggcgcgcta agactgatgg caagcgcaaa atcgccagat
    17401 gcgctacgct tatctggcct acgcggtcat tgtaacatat tgaatataaa cgctttgtag
    17461 gtttgataag gcgttcacgc cgcatccgac atgaacaaca tgcacttagc taattatcag
    17521 aggcacctcg taaggtgcct ttttttaacc gaagcgaaca tctcgtccat gcggttcatc
    17581 gagatcctgc cacggaccaa ttgaaataat ccccgtaggg ttgatggtct tatggctgcg
    17641 gaagtaatga ttacggatgt gatcgaaatt gacagtttcg gcgatccccg gcatctggta
    17701 gatatcgcgc aggaagccat acagattcag gtaatcgctg atgcggtgct tatcacactt
    17761 gaagtgggtc acatacactg gatcaaaacg caccagcgta gtccacaggc gaatatcggc
    17821 ttcggttagc tggttgccgg tcaggtaacg atgctggcct aaaatctgct ccagtcgcgc
    17881 cagcgattca aacactttcg ccaccgcctc gtcgtaggct tgctggctgg tggcaaaacc
    17941 agctttatac acgccgttgt taacggtgtc ataaatccag ccgttaagtt cgtcaatttt
    18001 cgtttgcagg gctggtgggt agtaatcacc cgcttttgcg cccagcgcat caaacgcggt
    18061 attgaacatg cggatgattt ctgctgattc gttgctgacg atggtgtggt tctttttgtc
    18121 ccacagcacg ggaacagtaa ctcgtccgct gtagtgtgga tcggcgtgga gataaagctg
    18181 atacagaaat tcatgttgat agagcgtatc tccggttgct cccggaaaac tgtcatcaaa
    18241 ggtccagccg ttttccagca tcagcgggtt cactacggaa acggaaataa acggttccag
    18301 tcctttgagt ttgcgcatga tcagcgtgcg gtgcgcccac gggcaggcga gtgaaacata
    18361 gagatgataa cgatctttct ctgcgataaa accgcctgtg ccagtggggc caggtgcgcc
    18421 atcggcagtg agccagttac gaaatgcgga agctgaacgt tgaaatttac cgccggtaga
    18481 tttggtatcg taccaggtgt catgccagac gccgtcaatc agttgaccca tttttctctc
    18541 ctccaatagc aaaaacgagg agcttcctcc tcgtttttta tcgttaagta tagcgcgtgc
    18601 ttaccacttt ttattcagca ggcggtcgat gctatacgcg cccggaccgg taattgccag
    18661 cagcaggaat ccgccagaaa ttgtcaggtt tttcatgaac atcagcgagt tgacgccttc
    18721 agcaaagttg ctgtgaaata aaaatgccgt cagcagcgta aagcccgcag taaacagggt
    18781 tgtggtgcga gtcaggaaac tgaataggat tgccagacca ccaccgaact caagcagaat
    18841 caccagcggc agcataaaac ccgggacacc cattgcttcc atatattgtt gggtaccaga
    18901 gtaagcggtg atttttcccc agcctgcggt aataaacaga atcggcatta aaatgtgcgc
    18961 taccagtaca ccaacatctt ctaatttttt catcatactc tccactgcgc cactcaggct
    19021 gctgattgtt tttttgtgca agggcgcggt attagcgtcg ttgctgtcga tggagagaat
    19081 cataaacgtg gtgaatgatg attgttagca aggaaaactg tcaaaaatat tcaaaaaatt
    19141 tgagggataa ggccggaatt gctccggcca gagggaagtt aaccgcgaag ctgttgctgc
    19201 ttgagggtcg ttttaaccag acgccaggcg ctccatacgc caaaaccgcg tctggcccag
    19261 cggaccagca tattaggatg gcgaatcgtc cagatcgcca tcacgctact gccaaccagc
    19321 gcccaggagc gcagacttag cagcatattc cagcgacgat cgtaagcgcc tgttgcctcc
    19381 agccattcac gacgactggc ggaaagatcc agccgttgtt gctggatctg gctaagcagt
    19441 tgcgccttac gtcgttcacg ttcgacttta ctgctcacgg gactcctcct cgagcagttg
    19501 ccgatcgttt gccagctcat ggcgtgtatg gcgcagcaag gtggatttac gcgatttacg
    19561 taacgtccag ataccgccaa tcagtgccag tagcagcaac accacggtgg tggcaatcat
    19621 cgcattcagg cgatattgcg gatcaaccgc ccaaataatt agcaccatca ggctcataag
    19681 accaaatgca gcgaaaagca tcgtcaggcc cagcatcagt aaaagttgaa agagattagc
    19741 tttttcctct tccagctcca ccaccgccag ccgcagacgt gtttccacca tttcaaccat
    19801 gatggaaaca attcgctgcc cgatgcccag aacgctttta ccgggccctt gcgcgtgatg
    19861 agtgtccgcc ataattaacg acgcgacagc agaacgccga gcactacacc gattgcagcg
    19921 ccaattccca cgccggtcca cggattttca cgcacatact catcagcacg cgccgccgcg
    19981 acacgggttt gtttggcgat ggcatcaccg gtttcaccca ggcgataacg gctctgtttc
    20041 agtgcctgct ccgctttgct acgaatctta ctcaactctt ctttcgactt ctcgccagat
    20101 gagctaagca cctcttccag cgtatcggaa agggatttca actcagcacg cagatgttcc
    20161 gtagtgtgtt ctttcgacat agttttctcc aggtgagtaa gtagtgacta ttgtgagtta
    20221 gtagtcgcgc gcttccagct ttttcagctc ttcctgcgct tctgccagtt tccgttctat
    20281 cggcatcgcc tttttgcttc gcctcggcta aatcttgctg gcgttccgcc acctcatctt
    20341 tctgctttgc gattttcttg tgatgatcgg cacgcagctg gctatctgaa cagttggccc
    20401 ggacttcact cagggcttta ttcagaccgt caatacgatt ctggttttgg tgtttttcgg
    20461 catagctgat ctccttaagg atattttgct ccttttcctg acacagggta gtggcataac
    20521 taccggcact aagagcaaag agagaaacag ctaaagcgat gcggtatttc attcttgaac
    20581 cttccattgt gattcggacc tattacaaag gaacgattgt ccagacaacg aagcggtagt
    20641 gttaaccacc gcctcattaa tcatagacag ttacggtgaa atcaccaaag tgagtgatat
    20701 gtttcggata acaggaagtt atccgaagcg atgagagtta tcccgtaacc gggtcagcca
    20761 ctgcatagcc tgactgttat tgtcctgctg cgcaatgatg tagccatggg gcaatgttct
    20821 gtcgaggacc gcttttgcgg cggcgctctg gtcgctggag tcaaatgtaa ttaacagagt
    20881 gtcgtttttg ggggtgatac ttttgaaagg aatgccatga gcgtcaaggt gatgccagat
    20941 cgaaaaaccg tctggcatcg ttgtgccttg atgaacggca cgaatcgcca gcgtagactc
    21001 ttgctggcga accgcggacc aggccagcaa cagcgtgccg accagaagta aaacagtgcc
    21061 ggaccaggct agctggcgaa gcgacatgcg aggtatttgc atcccttacc cccgatttcc
    21121 atatttcttt ttccataaca cgaccagaga acctgccagg ccaaacacca gcagcaccac
    21181 cgggagcagc atcaggcatg acatcagctg gtcctcgtac tttaaaaata ccggcgtttt
    21241 gccgagcatg taacccagag ttgtcaggat caatacccac agcagaccgc tcatccagtt
    21301 gaagaactga aagcgcgcgt tattcagccc tgataacccg gcaatcgtcg gcagcagtgt
    21361 tctgacaaat gcaataaagc gaccaattaa cagcgccgat aaaccgtgtt tatgaaaaag
    21421 atggtgtgcg cgttgatgat aatgcgcggg taaatgagat agccagtttt gtacggtgcg
    21481 ggtattgccc agccatcgcc cctgaatata gctgacccag caaccgaggc tggcggcaac
    21541 ggtcagcagc agaatcgttt gcgggtaacc catcgcgcct ttcgcaatca acacgccgac
    21601 caataccagt aaactgtcgc ccggtaaaaa ggccgccgga agcaagccat tttcaaggaa
    21661 caaaattaca aacaagacaa aatacaacat gccaatcatc gatggattgg ccagggtttc
    21721 aaaatcctgc gcccacaggg cttgcagcaa ttgggtcaaa agttccattc attattcctg
    21781 gtgaatcggt taacgccacg ctggttttaa aagatgcact accgcaatgt tattgctgtt
    21841 ttggtatgtt ctgcgggttt gatacatcaa cacagcctga actattccct gttaaaacgt
    21901 gagataagaa agcattcaaa agcataaatt caaattgaat ctaattgtaa caaaggtcaa
    21961 cgttgttcgt tatcaaaatt acatgtccgt ggagtctgat tttgctgatt gcaggggagg
    22021 gtggcgagag gatttacaca ggctgacact ttatactttt cgcttaccga cgagacaaac
    22081 aggcgcattg cagcgcctgt cggacaggaa tttcatttgc tgccgctggt ggccgtatcg
    22141 agatgcacta ccgggttttc ggcgaacaga tagcggtcgg cattgaactc gaaatcgtcg
    22201 ctggtttcgt taaataacat gattttggtg ttttccagat gctgccacat tgccagctta
    22261 gcagcatgag gatctttgcg aattaacgcc ttgagaattt gatcgtggtc atcacaccag
    22321 ttatcaacgg tacgggaatc aatgtgttcg tgtagttttt tccagtacgg gttatggcta
    22381 cgctgggtcc acattttttc aacgatggcc gccagggcgg agttctgcgt cgccagggct
    22441 acctgaatgt ggaactgtaa atcccactcg gaatcacgga agcattgttc gccgcgcgcc
    22501 tgttcctgaa tggccatcag tttcatgatg tcttgtttcg ttacctgagt tgccgcgaac
    22561 tcggcaatat tactttcgat gagctggcga gcctgaagca actcaaacgg accgtaattg
    22621 gcgaattcca tattattgtc agccgcctgc tgatgacgcg gctggttgga aaccacatga
    22681 atacctgaac ctttacgcac ctccacatag ccttcaactt ccagcatgat gatggcttca
    22741 cgaactaccg ttcggctgac gttcttttca tcggcaataa agcgttcggc aggcagttta
    22801 tcacccacca gatagacgcc ctgttcaatg cgctctttca ggtcagcagc aagttgttga
    22861 tacaaacggc gtggttcagt gatttccata tgcgtctccc ggagtggaat gcggacgaag
    22921 ttatcatacc actttattca tgggtatgcc attggtcata tataaatttg atgattgaat
    22981 taatgtatta aattaatatt gtgaatattt ataaattcct aaaaaataag tatcgtcatt
    23041 gtgaaagcta ctaaatttca gtaagttaat tcccagcctt taaatttaat gttaactaaa
    23101 aatgcttttt tatattgact tacaataaat ctacatctct aatacaccaa cttttttgaa
    23161 acgatattgt aagatcaaaa ttatatggtg gcgaaaatta atctcttatt acggcttacg
    23221 ccgcttttat tcgtgttcgc tccttttttg gcacagtcaa atatgactgt ttatcctatg
    23281 cctgtttcca ttaatagtca gggtgaaggc aacgttcgcg taatatctaa aagtaatgaa
    23341 gttcagtaca tcaaggcgac agtattccgt atcgataatc catcgacgcc tcaggaaaat
    23401 gaagttgaga ttaaatccgg tgatgcaaat catctggttg ttatgccacc taaattcgct
    23461 ttaccagccg gtagtagcaa aaccgtacgt tttgttgcga tggaaccaga gcaaaaagag
    23521 aaaaattatc gcgttaaatt tgaagcggtt cccagtattg atgacgttgc cacagataaa
    23581 aaagatctct ctatgcagtt aacagttaac ttaatttggg gaatcgttgt tagtgttcca
    23641 cctcagcaac ctatagctaa gttagaagta aattctgccc aaaaattaat taatgcaggg
    23701 aatcaacgct taaagctttt aacgatcgct tattgtaaaa ataatagcaa agaaaactgt
    23761 aagatacaga ccgtaaataa aaacatcttc cctggtcagg aaagaaatct tgaaagcata
    23821 tctggctacg acaaaatcgt tgtcaaatat aataactgga tcaccaaaga taatggcgag
    23881 tttgaactgg cggtccatta attatcattg taataaacga aggaatagta atgaaaaagg
    23941 tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc gcattggcag
    24001 tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg acacagaccg
    24061 ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag ggtctgcaat
    24121 cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta aaaatgcagt
    24181 tagtgtcccc agcgcaactg gttcaaagcc tggatgctag caagattgtt ccgctgacag
    24241 taacctgggg tggtgaagaa attaaagctg atgcggcaac cacttttacc gcaaccaaaa
    24301 tctttgcttc cgatgcgtta actaacggtt ctctggctaa aaacctgatg tttgctcaaa
    24361 ccaccaaagg tgttctggaa acaggcatct accgtggtgt agtaagcatt tatctgtctc
    24421 aggatattta atccggataa cagaattaac acgtaacaaa gggaggggta gccctccctt
    24481 attttaactg ttttagtatt tgttatggat ttcaggattg ccatggataa aaaattactg
    24541 gctcttttga tcctggcgag tctcagcccg gcagaggcga cattaaccaa aattcccgca
    24601 gggtttgagg ttattgctca gggacagcag gagtatatcg aggtttattt ttcagggaaa
    24661 agtctcggta aatattatgc aatggttaat cttgataccg taacatttct tgatccagca
    24721 agtctatata acaagctgga actggatgta gacgatcaga aaatcgcgca tatagtgaaa
    24781 gaaaaattat cgcagccgct agctcgccac ggtgaattgg cttgcggtta tgtacgtact
    24841 gactcagggt gtggttttct gaataccgat acgctggaaa taatctataa tgatgaagaa
    24901 agttcggcaa cgttgtttat taatccgcaa tggaattcag ctttcgatgc gaagtcatta
    24961 tatttaaatc cagacaaaaa tactgttaac gcttttatac atcagcaaga catcaatgtt
    25021 ctggcacagg atgattacca atcgttgtct attcagggaa acggtgcgct gggaataaca
    25081 gaaaatagct atattggtgc acactggaat ttcaacggtt atgatgcaga tgatgtcagt
    25141 gacagtaata ctgacgtcag cgatctctat tatcgttatg attttttacg tcgttattat
    25201 gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg cacaaggcgg gaactttacc
    25261 tttaactttc tgccactcgg tgcaatcgat gggatgcgta tcgggtcgac actgagctat
    25321 ttaaaccagg cgcaaagcca gcaaggaacc cctgtaatgg ttctgctttc gcgcaattct
    25381 cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt tttatctcaa tagtggttcg
    25441 caatttattg ataccagttc ctttccgcca ggcagctaca gcgtggcatt aaaagtctac
    25501 gaaaataacc aactcacccg caccgagctt gtgccgttta ccaaaaccgg cggtctgact
    25561 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
    25621 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
    25681 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
    25741 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
    25801 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
    25861 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
    25921 ttaagctgtt atgaaagtat ttccgcgaca gtttcactga attttgtcgg ctggaatatg
    25981 atgctgggtt atacctgcac acaaaataac actgatgata gtttgcgttg ggataaacag
    26041 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
    26101 caacttagcg cttcccgcgc tattgtgatg cgtgactgga ttttgagtac gtcagtcggt
    26161 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
    26221 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac caatgtgtct
    26281 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
    26341 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
    26401 gagacgattt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa caggatgaag
    26461 agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg cagcttggtc
    26521 aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt gaggttaacg
    26581 acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca gggaatcgtg
    26641 aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc tttaactaca
    26701 actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt gtggggctga
    26761 acagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt aacggcagcg
    26821 aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg agtgttgtaa
    26881 agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg acttattcac
    26941 aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta cgtggaaacg
    27001 ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg cgatattggg
    27061 cttgtttggc acgctcactg gcgttcaggc agctcctgac gtgaccagtg aaattacgta
    27121 tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt cggcaggaaa
    27181 taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct gtacagctaa
    27241 tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga tgcgttggcc
    27301 ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc cgctaggatg
    27361 caaggggggc aaagggcttc agacgaatct taaggattct aatagatctt catgtacaga
    27421 agatggtcaa cattattata tatacgatac aaagtttctt acgctctacc ttgagcagac
    27481 agagatgaag aatttgccga ttggtggcgt ctggaagggg aaagttaaat tacattcgaa
    27541 cagcccggcc caggactatt tcgcaaatat taccctgaat acgctcgacc ccaaccatat
    27601 tgacgtgttc ttcccggagt tcgcccacgc cacgccaagg gtgcagttag acttgcatcc
    27661 aacaggaagc gttaacggca gcaactacgc gcaagatctg accatgttgg acatgtgcct
    27721 gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag atgaagggcg
    27781 accagctgca gggcgcagag acggttactt ctctatctat cgtcagggag ggaccaccac
    27841 cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa ccggtgggca
    27901 aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga aacgtgtgcg
    27961 tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct
    28021 ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg gcaaattgtc
    28081 agtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg acaaacgctt
    28141 ttttctatat aaacgacgaa gccgcctgat aaggcggctt cgtcgttata acttacgtta
    28201 aacgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca atcgccggtt
    28261 tgttctgcaa tacggtccag ataaccagcg cacccagcag gtcaaatact gccagaactg
    28321 cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc gcaaacagcg
    28381 tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact tcgttacgac
    28441 cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtgg gcaaaaccac
    28501 cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc atacccgggc
    28561 caatcatcag cacagcaccc agcgttacga ccatcttacg ggaaacgatc aggttcacac
    28621 caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg aggtcagcaa
    28681 acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg taaactttaa
    28741 acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgct aggaaacgcg
    28801 gcagtgcgat accccagaac tgacggttac gcaggatctg accaacggac attttcttcg
    28861 ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt tcttcatcgg
    28921 tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc gcccagataa
    28981 agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg attgcccata
    29041 ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag tagcctactg
    29101 cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc gccgggatca
    29161 tcgctgcttc cgcggcaccg accgcaccac gagcaacagc caggccaccc cagctacctg
    29221 ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca taaccgattt
    29281 tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta taagcagcag
    29341 aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct tccatcagag
    29401 ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg ccgagcgtca
    29461 ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac ctcatcgttt
    29521 tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg ttagttacga
    29581 ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt tcgtgctaat
    29641 tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg caatttgcat
    29701 tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc ttaaaaggtg
    29761 agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag cgaggccagt
    29821 actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa ctacgtttat
    29881 tgatctaact cacgaaaata tcttcggact ctggaaattg gtgtgataac tttgtcagca
    29941 tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac tccgtttatg
    30001 actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga ctacgcaaaa
    30061 gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc ggaagactat
    30121 cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg gcgcgctatg
    30181 cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg tgaaaaattt
    30241 gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca ctggacgcac
    30301 ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc aactgccgat
    30361 gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctcctc gcgcggcatc
    30421 atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga ttctctggag
    30481 catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc gagctggcgt
    30541 ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc gaagctgggc
    30601 gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt gaccaaacgt
    30661 ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga cgtggtgatg
    30721 tttgctgaag cgaacgaagc ggaactcgac agcattctgg cgcgccgtct ggctggcgaa
    30781 accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt ccttggtgcc
    30841 gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg taataacaac
    30901 ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa cgaccgtccg
    30961 atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct gctgccgaaa
    31021 accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat gatcggtaac
    31081 ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg gtttaacgat
    31141 cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct gctgagccgt
    31201 tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca cgaatacttc
    31261 cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc accggcggac
    31321 atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg tgactacttc
    31381 gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc catgcgctgg
    31441 ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt gtcgataacc
    31501 agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta acggatatcg
    31561 caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg gcggatattg
    31621 cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat ctggatcagt
    31681 atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct gaagtgcaga
    31741 tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc ctgccaaccg
    31801 tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa gagaccaaca
    31861 acgccgaagg taccgacggc gtgttcctct tcagccacac ctacggctgc tcacaactgg
    31921 gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac ccgaacgcgg
    31981 gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca ttccgtgaaa
    32041 cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag caggatgatg
    32101 agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc aacgataaac
    32161 gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt tctgacggtc
    32221 tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg attgctaacg
    32281 gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag ttgctgatgg
    32341 accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac gacttcaaac
    32401 agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg aacaaagcgg
    32461 gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg ggttccagtg
    32521 tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg aacttgttaa
    32581 gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc tgccatatgg
    32641 tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg gtgaaaatcg
    32701 ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac gcgggtcagc
    32761 tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc atcgttgagt
    32821 ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg gcgatcttca
    32881 aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc gtttcataat
    32941 gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc gtattggctg
    33001 gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt caatcgtgac
    33061 gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt acattttttt
    33121 cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg tacattgatt
    33181 accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac tggcggaatt
    33241 ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac gattgtcagt
    33301 acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg gttttcaacg
    33361 tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat gattgagggt
    33421 agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca gaaaagggga
    33481 attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg ttaaacaatt
    33541 aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct gccgcagtga
    33601 acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat acgccgataa
    33661 taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac atattacagg
    33721 ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag gcacacagag
    33781 aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc agcgtattaa
    33841 ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg atggtggctc
    33901 ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca cacagcgcca
    33961 tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca ccgctttcgc
    34021 gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac accagcaatg
    34081 ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc agttgcgcgt
    34141 agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc ccaaaaatac
    34201 cgatcggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc gacatatcat
    34261 tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg ccgatcgccc
    34321 acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg gaaaccatgc
    34381 tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata tcacccgcgc
    34441 tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg gccagagctg
    34501 cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt ttctgcccgt
    34561 gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg gcaacagctt
    34621 tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg ggttttgaga
    34681 tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc aggctgccat
    34741 gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga tcctttcatt
    34801 gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt
    34861 ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc tggattttaa
    34921 agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca gagggaagga
    34981 ctggctatta aggctgatta gataacatat gggcaaaact atttggaata tgagaacggt
    35041 tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg cgcagggggc
    35101 ggccaatcgc cgccgccccc tgcaccctca ggctctggcg aacaaaatcg ccgctgcgcg
    35161 gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt ccctgtaaga
    35221 tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac caacacccgt
    35281 gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac ctttgtctgg
    35341 aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg ctgcttatca
    35401 tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc aaacaccacg
    35461 cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt gataccgata
    35521 aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc tacgcccgcc
    35581 agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt cagcacaatg
    35641 aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa aatcacgtcg
    35701 ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa tccattctta
    35761 cgcacaaaga aatgctcgtt gtcgatggtg tctgtcatgc gcaaatgacc gcgtagccag
    35821 cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc catcttcacg
    35881 ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg cgaaatcagc
    35941 cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac gccatacacc
    36001 agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat cagccagaca
    36061 aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa ggccagggcc
    36121 tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa agcggcgtta
    36181 aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt catggtcatg
    36241 gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat ggcgacaaca
    36301 acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt tccttacaca
    36361 taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata gacctcgcct
    36421 tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct ttcgggttgc
    36481 ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca atgaagttac
    36541 tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt ctgttacata
    36601 ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg aaaaatcacc
    36661 ccagtctggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac cgccagccct
    36721 ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc gtagagcatg
    36781 gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg cacaaagcag
    36841 actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc ctgaatctcg
    36901 cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa atcaccgtaa
    36961 tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc cgttgcctgc
    37021 acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa gccgatatcc
    37081 ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc gtccaggtaa
    37141 cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag tttgcctgct
    37201 ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt aaatgcctca
    37261 aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc cgccagattc
    37321 gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag ttgtgtctgg
    37381 gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc gctttccgcc
    37441 atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa gtgctgagcc
    37501 tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc atgggcggcc
    37561 tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat cataacgcgt
    37621 ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc ttcgacgtgc
    37681 gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc aatcatgacc
    37741 gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac cgcgctgaca
    37801 cgagcgagtt tctcccgcag ttgagtataa aaaccatgct tcatattgag gtctggcgcg
    37861 ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg ttggttatga
    37921 aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc acgcactacc
    37981 gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg tacgccttta
    38041 gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc gttatcgcca
    38101 ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt atcaatacgt
    38161 cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt acgcgccggg
    38221 acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac gcgatcggcg
    38281 ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag agtccggcga
    38341 gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt tatccttaac
    38401 gaaagggggg aggtcgattt taggcgctaa agaaagaaaa gataacgcct gacatcattt
    38461 tcagattttg tcctgcccaa gcgacaactg cgttagccac agccgggtgt cgaactcaag
    38521 ctggtggtac tgcggctcca tatggcagca caattgatag aacgctttgt tatgctcttt
    38581 ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc aaaaacggtt ccggcgcgtt
    38641 gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc agcttgccgc cctgcatacg
    38701 agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat tgtcatacat
    38761 cactttattg atcggcgggg cattacgcag aaactgattt ttcagatcct gagtatattg
    38821 ccagagagct ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg ccagcacatc
    38881 gcccagacgc tgttcgttaa tcagtgttcg cacctgcgaa agcagctgct cgggataacc
    38941 ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg gtatactcac
    39001 gcaccctttt caggggcaac gtcgaaattt tatcattcag gagggccgat gagccactta
    39061 gacaacggtt tccgttcact gacactacaa cgttttccgg cgacggatga cgttaacccg
    39121 ctacaggcgt gggaagcggc agatgaatat ttgctgcaac agttggacga cacagaaatc
    39181 cccggcccgg tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc gctggcggaa
    39241 cataagccgt acagcattgg cgactcatac atcagtgaac tggcgacgcg cgagaattta
    39301 cgcctaaacg ggattgatga gtcgagcgtg aagtttctcg acagcaccgc cgactacccg
    39361 caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct ggaacagcaa
    39421 ctgcgtgcgc tgcgcaaagt ggtcacgtcg gatacacgta ttattgccgg tgccaaagcc
    39481 cgtgacattc acacttccac gctggaactg ttcgaaaaag tgctcggccc gaccaccacc
    39541 acgctggcat ggaaaaaagc gcgcctgatt aattgcacgt tcaatgaacc gccgctggcc
    39601 gatgcgccgc agaccgttag ctggaagctg gaaggtactg actggactat ccataaccat
    39661 gcgaatgtct tctcccgcac cgggcttgat atcggcgcgc gcttctttat gcaacatctg
    39721 ccagagaatc tcgaagggga gattgtcgat ctcggttgcg gtaatggcgt tattggtctg
    39781 acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc gccgatggcg
    39841 gttgcttcca gccgtttgaa cgttgaaacc aacatgccag aggcgttgga tcgcagcgag
    39901 tttatgatca acaacgcgct ctccggcgtg gagcctttcc gctttaatgc tgtgctctgc
    39961 aaccctccgt ttcaccaaca acatgcgctg accgataacg tcgcctggga gatgttccac
    40021 cacgcccgcc gctgcctgaa aatcaacggc gagctgtata tcgttgccaa ccgccatctg
    40081 gattacttcc ataaactgaa gaaaattttc ggcaactgca ccactattgc gacgaataat
    40141 aaatttgtgg tgctgaaagc ggtgaagctg ggacgtcgtc ggtaagattg ggagttacct
    40201 gccggatgcg gcgtaaacgc cttatccggc ctacgttcag cacgttaccc ccaggccgga
    40261 taagacgcgg caagcgtcgc atccggcaat gtctgctcag ttctggaatc gcgcttccat
    40321 aaaacaaaaa atccctgtca ccatcctcta cattctcttg tttagcgttt ttctacgttt
    40381 attcttccgt cacacagata aattcatccg ttgccaatct gtcaacggat gttatcatat
    40441 tgccaatttg tcaacggacg tgatgaagaa tgcacctgat aactcaaaaa gcattgaaag
    40501 atgctgcgga aaaacacccg caacataaaa cggagttggt ggctctgggg aacacgattg
    40561 ctaagggata tttcaaaaaa cctgagtcat taaaagcagt attcccatct ctggataact
    40621 tcaaatatct ggataagcat tatgttttca atgttggggg caatgaatta cgtgttgtag
    40681 caatggtctt ttttgaatcg caaaagtgct acatacgtga agttatgacg cataaagaat
    40741 acgatttctt taccgctgtt catcgtacta aggggaaaaa atgattgcga ttgccgacat
    40801 cttgcaagca ggagaaaagc taactgctgt ggcacctttt ctggcgggta ttcagaacga
    40861 ggaacaatac acccaggcgc tggaactggt agatcatctg ctgctcaacg atcctgaaaa
    40921 ccccttgctg gatctggtgt gtgccaaaat aaccgcgtgg gaagaatcag cgcccgaatt
    40981 tgcggaattt aatgccatgg ctcaagccat gcctggcggt atcgccgtga ttcgtaccct
    41041 tatggatcaa tatggtttaa ccctttccga tctgccggaa attggcagta aatctatggt
    41101 gtcacgcgtt ttgagcggga agaggaaatt aacgctggaa cacgctaaaa aattggcaac
    41161 gcgattcggc atttctcccg ccttgtttat tgattaacgc gacgtgccgg atgcgttgca
    41221 tccggcgctt caaataacga tttaaatctc cagcgccagc cgtgttccct gggcaattgc
    41281 ccgtcgtgcg tccagctcca tagccacatc gcagccgcca attaaatgca cagttttccc
    41341 gctatcaatc agcggttgcg ccagcgcgcg gtttggctct tgccctgcgc agatcaccac
    41401 attgtccact gctaatacct gcgtttcgcc gttgatcacc acatgcagcc cgtcatcgtc
    41461 aatcttctga taacttacgc ctgggatcat tttcacaccc cgcgagagca gggtggtgcg
    41521 atggatccag ccggtggttt tgcctaaccc ctgtcccggt ttgctggctt tgcgctggag
    41581 catcacaatt tgccgtgggc tacgcgggat ctgcattccc tgcgggctta agccaccagc
    41641 ctgttgtagg ctgctgtcga tcccccactc attacagaac ctggcgatat tctggctggt
    41701 ggattcgccc ggctgactta aatacatcgc cgtatcaaaa ccaatcccgc cacaaccgat
    41761 gatggcaacc ttgttgccta ccggcgcttt gtcgcgcagt acatcgagat aactcaatac
    41821 cttcggatga tcgatcccgt cgataggcgg aacacgcggc acgatcccac tggcgaggat
    41881 cgtttcatcg aacgcctgta actgatccgc cgtcacggtg tgattgagtt tcagcgtcac
    41941 gcccgtcact tcgatcatcc ggcggtagta gcgcagcgtt tcgtaaaact cctctttgcc
    42001 ggggatctgt ttggcgatat taaactgccc gccgatctcg ctgtgcgcat caaacaatgt
    42061 tacctgatgc ccacgcgccg ccgcgttaat ggcaaacgcc agcccagcag gtccagcgcc
    42121 gaccaccgcc agattttttt tctgcacggc gggaaggatt ggcattttgg tttcgtggca
    42181 ggcgcgagga ttcaccaggc acgaggtgac tttgccaacg aagatctgat cgaggcaggc
    42241 ctggttgcag ccaatacaag tgttgatctc atcggctcgc cctgattgcg cttttgacag
    42301 cagttccgca tccgcgagaa acggtcgcgc catcgatact atatcggcat cgccgcgcga
    42361 gagaatatcg tcggcaacct gcggatcgtt aatccggttg gtggttacca gctgcagcga
    42421 gacgtggcct ttcagtttgc gcgtgaccca gctaaatgcg ccgcgcggca cgggcgtggc
    42481 gatggtcgga atccgcgctt cgtgccagcc tattccggtg ttgataatgg tcgcgcccgc
    42541 cgcttcaatg gcctgcgcca gttctaccgt ttcggcaaaa gtgccgccgc cttcgaccag
    42601 gtcgagcatc gacaaccggt aaataataat gaagtcgttg ccgacgcgtt cgcgcaccgt
    42661 acgcactact tctacggcaa atcgcatccg gttgcggtaa tcgccgcccc actggtcact
    42721 acgctgattg gtgcgcagcg tcagaaattc gttgatcaaa tacccttcag agcccatcac
    42781 ttcgacgcca tcgtatccgg cctcccgcgc cagttgcgcg cagcgggcga aatcgtcgat
    42841 cagttgcagg atctcttcat gggtgagttc gtgaggaacg aaacggttga tgggggcctg
    42901 caatgcggac ggtgcgacca gatgcggttg gtagctgtag cgcccggtat gcaaaatttg
    42961 cagggcgatt ttgccgcctt cctgatgtac ggcttcggta atggtgcgat ggtgtgggat
    43021 ctggctggcg tcgttgagca ttgcgccacc ttccatgcca acgcctatta aatctggtgc
    43081 aatgccgccg ctgacaatca gcgccacgcc gtgacgggcg cgttcggcat aaaacgcagc
    43141 cagccgttca gcaccgtccg ggtattcctc cagcccggtg tgcattgaac ccatcaacac
    43201 gcggtttttt aacgtggtaa aacctaaatc cagcggggcg aacagcgacg ggtagctcat
    43261 aaaattgtcc agtatgtaaa ataattgtta tgtggtcgga tgagttctaa tttagccgtc
    43321 gatggagaaa agggaaaagg ggagtgcggg gtttgtgatg ggattcaaaa agaatacgcc
    43381 cggtggtaac gccggatgcg acgcttgccg tgttttatcc ggcttggggg attgcacatg
    43441 tagggcggat aaggcgttca cgccgcatcc gccagccgtt gcaaattact gcttacggaa
    43501 aaaatcgtta tacaacatat acaaatgagc ggcactccag gagaaatttg gtgcgccttg
    43561 ctgcgcgcct gtcagcgggt tgtaattctc ctgaattggg ccatcggcgg ttaatccttt
    43621 ggcgtgcctg aagaacgtat ccgccagctt cagggcatca tcacgataac cgtaacgctc
    43681 catccctttc agaccaaacc agaactgatc cacccatacg cgcccgcgcc agtagatatc
    43741 agcgccaaaa gccggattgg ttaacgccgc cgttcccagc ggaacaaagg tgttgaactc
    43801 tttaggatcg agcatcacct tcaccaccgc gtcggcattg gcctgcgttg ccgcaccgtt
    43861 aaacagcggc gaccagcctt ccggcccttt accgcgctca acaatcggtt tgcccgcgca
    43921 gccgttcgcc agtggtttat cttcaatacg tacgtcatag tagaactggg tagtcgggtc
    43981 gaacatacag gtgttgatgt agtccgcgag ctgctgtgcc aactggcgat agcgtttggc
    44041 ctcttctggc ttaccgagga tggttgccat ctccgccaga taatggttat cgctgtacat
    44101 atagctggcc tgatccaccg actcctgcaa tagcgagtag cccagcaacg ttccgtcctg
    44161 actgcggttt tcggcgaatt tcaccgtcca gtcgctacgt ttgccgccat tagcgacata
    44221 tttatccaac tgttctttgt cgataaaacc aaagacggcg gcatcatcac gccccgattc
    44281 ccacgacgca gcgacctgtg ccggaatttc cagactgtcg tactggcctt tctccaccac
    44341 gcgggcgtag ttgttcagcc cagactgcgt ctcttcttta tcgccttttt tcaccgtaaa
    44401 cagcatctcg ccgctctcgg tgttgtgggc tttgtcgcgg gtcgcgccat attccggcac
    44461 cccgttgccg ttatgatcgc ggttacgtaa ccaccagtcg tgataggcca ccagtttcgg
    44521 gtacatctct gccaaccagg ctttatcctg ggtgacgttg tatacttcca tcaccgacca
    44581 ggcggcaagg ctgggtttgg tattacgttc gttccagttg ccgccatcgc caccacgctc
    44641 ggggctaagg ttccacgcta tcaggtcggg gacaaagccc acatcctgcg gacgcacgct
    44701 gtcgccaggc tggatctgcc aggagaagac cgcgcggata ttctctttgg cgatatccgg
    44761 attgaaatgc gccatcgcaa acgcctgctt ccaggtatcc cacggccagg tctgattgcc
    44821 ggagaaccag cgtccggtca ccgacggcgt gacggtgtta taattcaccg caccgcccgg
    44881 tgagcgccag ttaccgttga gcgtttcgat cgctttcacc gcgacgcgcg tctgttccgg
    44941 cgtcgcatcc ggattggtta accctttctt caggtattct tcccagcgtt gctgcgaggc
    45001 ggtgagataa aacgccggac gcgccagaat atcgcggatc tgcatttgct ctttgctaac
    45061 ttcctgagcg gtcagcagat gggaataggt ggtgtagagc gtggtcgaac cgttgatatg
    45121 cgccttactg gtaaagcgat taccgttgat ttcagtctgc accggcaggg atttatgcac
    45181 ctgatattcc gattcgccgg aggtcagcag atcccaggtg gcgcgcactt tgccaaaggt
    45241 gactttcagg ccatcacggg tggcgctgat tttgcgctga tagtcagggt attcgccagc
    45301 aatggtttta tcggaaagcg gtttcccttc tttcgcttcc agtttttcca gcagttcgcc
    45361 atcccacacc agatccagcg gtttattgct gatgatttta gtttccagta gtgacgtgcg
    45421 cggcgtggcg aagcgcagag tcatttcgac ctgcacatct tttgctgtca gtttttgcac
    45481 cagcgcgccg ggaatactgt atgcctccag cgtgaagttg actttcttgc cgtcttgcca
    45541 gacagtcagg cggtcgaaat tgctggccat aaagttgatg tattcttccg tcagcagcgc
    45601 aacgcccgga aagccgccca tggtgttcgg gccgtctggc aacagatgac catgccaggc
    45661 tccgagatcg aaaaacggat taaaacgctg gtgatcgtcg taatcgtaat ccttcatgta
    45721 ctgcggcgca ccagtacggt taatcacgtt tttataattg tcggcgttag cggcatgagc
    45781 ggaaaaactt atcagcagag cgcaggttac tggcgttaaa atagttttga ttttcatgaa
    45841 attacagtcc ttattaccag tagaaatatt gcatcaggaa gatttggtcg ctgggatcgt
    45901 tagaggcatt tttcatgtag aagcggctat caaaagccgt ggcgaaacgt ccgccgccat
    45961 attcgtacca gataccaaac tgcatgaaag aggtggtttc atcgtcagcg tggtcagggc
    46021 gatgtttggc gtaggtgtaa gcggtggaga gataaacgcc ctttaacgct tcatacatgg
    46081 cgctcaccat catcccgctt tcacttcccg gtacgtccag cccgtcggca cgtccggtat
    46141 ggtgccaggc gcgagcggca aagttagggg taaacaggcc cgtgagatag agttgtcctg
    46201 tgccgtcggt aatttccaga ccattcatcc aggtgatgcc ttcggcaagg tcatactgga
    46261 tgtaaccgtt catccattgc cggataggtg tatttgtcgt cgtagcgatc gtacttgcca
    46321 aaatgtagcc aggctttgct ttcgtcatga tgtccggcag gtgtggcggt gacgctataa
    46381 cgaaagttgc cgcttaagtt ctgtaccttc agcgcggtca taatgtcgcg ggtattggga
    46441 atgacgtagc ccaaatcggg tgtgaagtcg ccccaccatt gcaggtcatc cagcgaggaa
    46501 tcctgacgca gactgagcat gatttcggtg ccatcattgg tacgataacc gccatagaaa
    46561 cggttaatcc caccttcgaa accaccccag ctatggtcag gcacctaggc attaccgtcg
    46621 tggtcggcct gcacggtcca gccttcgccg taaatcaggc cgaaccagga gccgtacttc
    46681 atctccatgc caccttcaat ataggtgccg tcgctgtatt tatgtttatt ctcaccctct
    46741 aaatccatat atccaccaag ccctaattcg ccgtaaaaac gaaatgaagg agaagatgtc
    46801 gaggtaattg cgggtgtttt tgtgtcgtca tgttctgccg gacgtgcttc ttctcctgcc
    46861 gcagaaaagg cgtagcaaaa cggcagagca agtaatgctc tgcatggtgc agtgattaat
    46921 ttcattgtgt taatccatga gctaaagaaa aagtacagcc ttttatcgtt gtaattataa
    46981 aaatataaag gcagaaacta ttttccctct cctcatccag gaagagggaa aatgcctgct
    47041 cattttttat tagtctgctt aatcaacatt gctggctggc gtcgcttcaa tatggcgctc
    47101 ttcagccgtt aatcccttta tatatttact gtatttccac cacgcaaagc cgaggaagat
    47161 aacaataccg ccgacgttat aaaaaatgat ggtcagaata ttcgcgccag tcgggaatgt
    47221 cgaagcgaca aaccctacgg caaatatcgc aatcagcatt gaaacaacga taataccggt
    47281 gcggcgggag cccatacgga aatcgcgtgg caggtgatct aatttggcgc gtaaattcag
    47341 gtaagccagc atgatgaata acggcggaag catggacgct gcggcggtca tattaataat
    47401 ggtattcatc agatcctgca cggtattgga accgagcatc gggataatca tcagcgggat
    47461 gacgatcaga aactggatcc atgctgcgcg cgccggaacg ccgttttcgt taagttcgac
    47521 ggttttctta ccaaagatgc cttccgggat ttcggagaag aaaattttca ccggcgttgc
    47581 ggtccacatc agcagggaac cgaacattgc ggtaaaggac accagaccga caaagcgatt
    47641 catcaacgct tccggcagac caaaatacgc cgccatgccg tggaatacct gcaccgatcc
    47701 gccggtaaat ttcaactctt tgctgctgac gaagacgtta atcagcaccg aggagacgga
    47761 atacagtacg ccgataaaaa tcccggcgag gatgatcact ttaacgaacg atttcgaacc
    47821 gcctttgacg tcgttaacgt acaccgcgac ggactccgcg ccacctgcgg ccataaagat
    47881 ccaggtggta acgccgagga acgcccagtt gaagttcggg atcatcgcgt caacggtgat
    47941 cggatcggca ggctgtacgt cgccaaccag tgccgtacct gccagtaaaa tgtaggagag
    48001 cgttaacagc agcatcagcg ttgaagtgac ggaggtaatt ggccccagca ttttcgcccc
    48061 gttggtggaa acccaggtgg agaaggcgaa cagcaccata ctgataacgg tggtggcaac
    48121 cggcgtcata atatattcgt agccgaggaa ggcatacgaa gcataagcaa taacgcgcgg
    48181 caacagcgag gtaaaaaaga acagatttac gaaccagtaa gtgtaggcag taataaatgc
    48241 ccaacgtccg cccagcgaac tttttaccca cgcgtagaca ccggcttctg agtttttatt
    48301 taacgaaaca aattctgcga tgatcagaca gaagggaata aaataaaaaa tcgtcgcgag
    48361 gaaaaacatc ggtgccgagg ccagtccaag ctcaatatta ttattgataa cgttgttaaa
    48421 gctgtaaacg gcggcaaaag tcagcgagag caagccgaat ttgccgattg tattacgttt
    48481 ggtatcagac ataacacatc tccgaaaatg tgtagggtag cggtggcggc aaactccttt
    48541 tgccgccgta gttgttattt gttatggaaa taaccatctt cgatggtgac tttgagaacc
    48601 acttttttga ccgcgttatt gcagataaac cgatacgctt catggatatc gcaaataacg
    48661 atttgccctt cgtggacctc tacggtttct ccgcagcctt ttaaatattc acggtcagtt
    48721 tcatcacgat aatattccac tacctgtaat gtctctttcg gcgcatattc aattttttgc
    48781 tgcccttgca ggtagtaatg cacttcaaaa tagcgtcgat gcccggtaaa tagcgcatcg
    48841 gtcgcggagt cattttccac acggtaggtc aatgaatcac cgatggagtg ggcgacgcca
    48901 ggctgaatgt tgtcgatatt ttcaatcgct tcaacgcagc gttgccactt cttgccagag
    48961 gcgtaaatct ggcggaactg ttctaagtta tcgatgatcc tcattgctta ttctcgctgt
    49021 gcaaattcgt ggaaaagaac cctgcgccga actcatacga cgccaggctt tgcgcggtag
    49081 cttctccgcc agaaaccggc agcaacgtaa agccgtagct gaagtcacgg aaccagacgc
    49141 gccaggagtc cagcacctcg ctgccccagg agttggagcc gaggccaagt agctggtggt
    49201 cgaggttcag ggtgatgtca tcactgcgct gcagctcgtt acagtgctgg gcagcgtgga
    49261 tgttttcctg ggtatagtgc caggcgctga agttaattgg gcgctgcgga accaccagca
    49321 gaccgttgtc gtggcggtta gtcagtgccg tccagcggac atgctgacgg ttgccgttgt
    49381 tctgcgggaa gggatagttc tcgaacatgg catcgacggt gctgcgccag atatcgatga
    49441 tgttagcctg ctggctgtcg gcgtagtttt cgcccggtcc acgaccgtaa tacgccacct
    49501 gatcgtattc gccgttaatc cccatggtga aaccgatgca cggaatgatg tgcggatagt
    49561 cgccgtaacg ctcgccggaa agcgccacgt taacctggcc atcggcagcg atgcgccaga
    49621 tgtaggtaca gcgcatcccg aagtcaaaca ccggcggtgc aataactgtg cggttgatga
    49681 tcaacacttc accatcgctc tgttctacgg caaagtcgcg cagatgttcc tgcatgatct
    49741 gcaaatgatt cggctgccac aacccttcgt actcctgctt gtggttgtcg atcatcggct
    49801 tgaagaagtt gatctttggc tcgcgggtca gcagcgactc gccatttacc tgccaggatg
    49861 tcggtttgcc actcattttt gagaaggtga tcgcgaagtt gtagccgcga acggtgcagc
    49921 tcaaacgatc gtcttccagc gtcagcggac gcgcattatt tggtgcgaaa ggcactggct
    49981 gcgcggtgtt ttccttcagc gggaactgat aagtggcgat agaatgcccg gcttcgctgt
    50041 agcgggtgcg ggaatctttg gtcaccgtaa tgttgaggaa cgcttcgcgg gcgtccagct
    50101 gcggcagcgt gatctgcaag ggggcttcgc tgttcggcgc aacgtcgcgc agtttaatct
    50161 gctgcgtcgc gagcgtttca ccttcggcgc gcacctctgc gtgcagggtg tagtcatcaa
    50221 gcgtggtaaa ccacagttta ttttcgactt tcaattcgcc gcgagtcagc gcgtggattt
    50281 ttaccggcgc gataacctgt ttgtactctt tcaggcccga tcccggcgtc tgatcggaat
    50341 agatcaaacc atcaagacag aagttatagt tgttgggata gtcgccgtag tcgccgccga
    50401 atttatacca gacattgccg tggtcgtcct gtgcctggat cccgtggtcg caccactccc
    50461 agacataatg accctgaatg caatcgtgct tatagaagac gttctggtac tccgtcagcc
    50521 cgcccggtcc gttccccatc gcatgagcat attcacagat gatgcgcggc ttcggatgcg
    50581 ggtattcacc aaactcattc atcagcggca cgcgggtgta catggtggaa ataatatcga
    50641 ccacttcagc atcgcgatct tcttcgtaat gcaccagtcg cgtgtcatcc agcgctttcg
    50701 ccgcatggta catcgcgcgg atgttacagc catagccgga ttcattgccc agcgaccaga
    50761 tgatgatcga cggatggttt ttctgtgcgt ggatatggcg aacaatgcgc tcgacgtaga
    50821 ctttttccca ctgcggatcg tcggtaatac ggctgatatc gccgacatta gcaaagccgt
    50881 gcgattcgac gtcggtttcc gccatcacaa acaggccgta gatatcacac aattcgtaaa
    50941 aacgcggatc gttcgggtag taagcggtac gcacggagtt gatattgtgc tgcttcatca
    51001 actggagatc tttctcgacg cgatccattc caacggcgcg gcctttgcga tgatcgttgt
    51061 cgtgacggtt gacgccgtgc agcatcacat aacggttatt gatccagaac agaccgtcgc
    51121 gcactttgat atcacggaag ccaacgcgtt gtggcaccac ttccagaacg ttgccgttgg
    51181 cgtctttcag cgtcatgacc agatggtaaa gataagggga ttctgctgac cattgctgcg
    51241 gctgttcgac agtaaaagca aagctggcgc tggtcagttt ttcaattgcc aaatgatcaa
    51301 tggcgctgct gtgcaccacg cgttcgccat caaacagggt atattccagc gtcgtgacga
    51361 caggggaggc ggcgagattt tccagcacca cttcgcagga aagcgtggca tcgcaatagg
    51421 cttcgtcaaa gtcggtacgc acagtgaaat cgttaatatg cgttaggtgt tttccgacca
    51481 gataaacatc gcggaagatc cccgccgacc accacatatc ctggtcttcc acgtaggtag
    51541 agtccgccca ctgcatcacg cgcacacaca acaggttgtc gccggtttta accatcgcgc
    51601 tgatgtcaaa ctctgcggtc aggcgactgc ccttgctgaa acccacatac tgaccgttaa
    51661 cgtagacttc aaaataggtt tcgacgccgt caaatttaat cagcgtctgt ttaccctgcc
    51721 agccgtcgct gagggtgaaa atacgttgat aggcaccggt tgggttatcg ctggggacaa
    51781 acggcacatc gatggggaac ggaaaacctt cgtcggtata ttgcagtttg ccgtgacctt
    51841 ccatttgcca catggcgggg acggtaatat gcccccagtc agccattaac tcagaggtga
    51901 aggcttctgg tacttgcagc ggatggtcaa aaaagtggaa attccactga ccgcttaagg
    51961 gcagaaacag gctgctggtt tcgcgggcaa aggtacgcgc ttgcgcaaca gaatcatatg
    52021 aaaaaaagta cgcacgcggc gcaagtcggt tttcgtgggt gagctgaatg ttttcccagc
    52081 gattcatagg gcctcccggt agagagaaat cggaaccatt catggtgatg gtagtttgtg
    52141 taattgctgg gggtttagta aaatttttac taaaagtagc aggtaaatgc atttacttta
    52201 actggatcac gaaaataaac ggcagcactg tccggcaaaa atgattgccg gacagtgtgt
    52261 aaggggattt aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa gactagcagc
    52321 ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt taacgccctg acttcccatc
    52381 atctcggaat ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc ggtggggata
    52441 tcgttaacgc tgataagcga aatatcttgt gggatgttca ggccacgttc atgaattgcc
    52501 cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg atagtcttcc
    52561 cgcgccagca tttgttttgc cagttcataa cccgacgaac tggaaaaacc gccgcgccag
    52621 atatcctctt cgcgtaccac ttgtttcagt cggccatatt ccgcaaaggc gacctcacga
    52681 atatccgcct tgccaggctc atcttcaccg ccaataaaac caatacgatt aacgccctgg
    52741 ttgatataga agtcgatgat ttctttactg atgcgtgcca gatcgatatc caccgcatcg
    52801 taaccgctgc cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa cgcgctggca
    52861 gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa taccggtgac gtttttaatg
    52921 tctggtaatc cgttgtgttc ataacagttg ttcagttcga tcgccagctt ttcgcactga
    52981 gtttcaatgc cgtggcggat cgccagatag taaggatcgt tgatctccag ctcctgctgg
    53041 tagctgtaga tagccagaat atggtgttgg ttgactgcgc ctgtctgtag tttacgggcg
    53101 ctactggtct tgtactccag cttttcggcg atctcgagaa tgcgatgttt cgtctcttct
    53161 ttcacattca atgtcggatc gtcatttaag accctggata ctgtcgccag ggatacgcca
    53221 gcttcgattg cgatgtcttt taatgttgcc atgtttacct tttttacttt ccctgatgtg
    53281 cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat aaagcgcgtt
    53341 tctttgacgc tgtaacggta acacagcggc ttaggaatgt gatcacgcct gcattctcca
    53401 tttttagcct acaggattga gaaaaacttt tgctatagtt tagtaaaatt ttacccgaag
    53461 ggaggaagca atggaaaccg tggcttacgc tgattttgca cgtctggaaa tgcgcgtcgg
    53521 aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag ctgtacatcg tacaggttga
    53581 tgtggggaaa aaacactgca aaccgtgacc agcttggtgc cgtactacag cgaagaggag
    53641 ctgatgggga aaacggtggt ggtcttatgc aatctgcaaa aagcgaaaat gcgtggtgaa
    53701 acgtcggaat gcatgctgtt gtgtgcggaa accgatgacg gcagtgaaag cgtgttgtta
    53761 acgccggagc ggatgatgcc tgcgggcgta cgcgtcgtat aaaaagatcg gatggcgacg
    53821 tcgtatcgcc atccgatttg atattacgct tcttcgacac ttactcgcat ggccgccagc
    53881 gccttacgcg ccgctttgat caccagttca cactgttcaa tggtcagtgt cagtggcggt
    53941 tcaatgcgga tcgttttggc gttattgagc gttccggcca ccagtacgcg ctggcggaac
    54001 atctcgctgg caaagttata gccgatttcg ttatcaacaa actcaatcgc catcaacatc
    54061 cctttaccac gcgcttcctg taccagatcg ggatattccc gcgccagttg acggaaaccg
    54121 tccagcaaca cattgatggt cgccagcgcc gccgcacagg ccagcgggtt gccgccaaag
    54181 gtggtggtat gcaggaatgg gttgtcgaac agaactgaaa acacctcttc agtggcgatg
    54241 gtcgcgccaa tcggcatcac gccgccgccg agggcttttg ccagacacag aatgtccggc
    54301 tgaacgttct catgttcgca ggcaaacatc ttgcccgtgc gccccatgcc cgtttgtact
    54361 tcatcgagga tcatcagtgc gccgaactca tcgcacagct tacgcacggc ggtgagatag
    54421 cccggcggcg gcagaattac gccaccttcg ccctgaatcg gttcgaggat caccgcagcc
    54481 acatcatcac cggttttttt gcactcatta agagccgtgc gcatggcttc gatattgcca
    54541 aacggcacat gacggaaccc tggcagcagt ggcataaacg gtttgcggaa ggtcgatttc
    54601 gccgtggccg acagcgcgcc aagtgattta ccgtggaacg cgccgctggt ggcaataaaa
    54661 gtaaatttgc cgcgcggcga ctgataagcc tttgccagct tcagcgccgc ttcaacggac
    54721 tcggtgccgc tattacagaa gaagctgtat ttcagtttac cgggcgttag cgcagcaagg
    54781 gttttcgcca gcatagcccg tagcggatcg agtagctcct ggctgtgcag tggttgtttc
    54841 gcaagttgat tctgtacggc ggaaaccaca actggattac ggtgccccac gttgaaaatt
    54901 ccaaaacctc ccaggcagtc gataaactcc tgtccctggg tgtcgacaag cgtatttaaa
    54961 cctcccgctt gccactctac ggctccgtaa tccccgccgg cggtaacaga tttgcgatac
    55021 tctaaaaacc ccggattgac atgctctttg aagtattcaa tcacctctcg gttaagtgct
    55081 ttcatctcct catgatccag cgttcgcttc tcaatgagat tcagggcgtg ggcgctgcac
    55141 gctaaagccg atgcgctcga aggtaacctg ttcaaaatat gctccggagg ctcgcgtatc
    55201 acatgatacc gatttaagta ttgcagggat tgcgccactc cggctgcgat cgcgaaaatc
    55261 gggataaaca caaatgcggt tatcatttgt gcaatattta atgctatccg ttaacatttg
    55321 ttgttatttt gcacggtttt gtcgggtatg gatttgatga ttgctgcgca aatgcacgtt
    55381 ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt atagtaacag
    55441 agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat tgcgatctaa
    55501 atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg acaagaagtt
    55561 aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg tcacccagca
    55621 aaataccccg ctggcggacg ataccactct gatgttcact accgatctgc aaagctatat
    55681 cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag agttacaagg
    55741 gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg cggatatgtg
    55801 gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc gcaaaaatgg
    55861 cgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa aaatcagtgg
    55921 ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg agccgctgta
    55981 caaagcgttg aactccggac gtaccagtaa gcgtattcat aaaggcctgg tggtgcgtaa
    56041 aggctggctg ggtaaactgc cttcattacc gcttcgctgg cggacgcgtg gagtgatgac
    56101 cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg tggtgacgta
    56161 tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc agattgtgcg
    56221 cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc gtaatagtgt
    56281 tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag ggcagcttgg
    56341 cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg tcagaaatgg
    56401 cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc agacagttga
    56461 taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga aacagaactc
    56521 tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg cagtgcaggg
    56581 tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca gtacccagcg
    56641 cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata ttctggccct
    56701 gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg cagtggtggc
    56761 aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata ttcgcaagct
    56821 gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg ccgccggacg
    56881 gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg cccagattag
    56941 ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag tggatgagct
    57001 taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg tgtcggcgat
    57061 ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt aatcggtatg
    57121 ccggatccgg cggttggagc acaatacctg atgcgatgct ggcgcatctt atcaggcctg
    57181 cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc cggcaattgc
    57241 accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa gcggcgcggt
    57301 cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg ttgacgcgta
    57361 aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc tggcgcagcg
    57421 ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt tcgcggtttt
    57481 cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca ccttcctctt
    57541 cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgtcgggtag atgacgcccg
    57601 ggcttggggt gtaattcccc tgggttagat tctcaatcgc tttaatcaat tcgtaaccgt
    57661 ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg tgaccaaaga
    57721 aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcaacattga ccgtgttcat
    57781 gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag cagccctcat
    57841 ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata tcagcctcct
    57901 tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca agtacaaaaa
    57961 tgatttttaa acgtggaagc acctcacatc taggccggat aagacgttca ctccgcatcc
    58021 ggcagcattg ctatggcact gaatgccttc acatacttca gacatatcag ttctacaaat
    58081 cgcttgcatt tatcatgatt aacaatcatt atcatttgcg aattttattt agatatatct
    58141 gattaccatc acgaaggcga taacaatgaa taacaccccc cgctacccgc agcgcgttcg
    58201 caatgatctg cgcttccgtg aactgactgt gctacgcgtt gagcgcatca gcgccggttt
    58261 tcagcgcatt gtccttggcg gcgaggcgtt ggacggtttt acatcgcgcg gctttgacga
    58321 tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa cggtaacgga
    58381 agagggcatc gtctggccgg aaggaccacg cccaccgtcg cgtgactata cgccactgta
    58441 tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg gggtcgccag
    58501 cggctgggcg ataaacttac ggtggcaggt ccgcgcggtt cgctggtggt gccggaagat
    58561 tacgcgtatc agctgtatgt ctgcgatgaa tccggaatgc ccgcactgcg ccgccgcctg
    58621 gaaacgttga gcaaacttgc cgttaaaccg caagttagtg cgctggttag cgtgcgggat
    58681 aacgcctgtc aggattatct cgcgcacctt gatggtttta atatcgaatg gctggcacat
    58741 gatgagcagg cggtagatgc gcgtctggcg cagatgcaaa tccctgccag atgattactt
    58801 catctggata accggcgaag gtaaagtcgt taagaattta agccgccgct ttgaagcgga
    58861 acagtatgac ccacagcggg tacgtgcagc ggcttactgg cacgcaaagt aactgatata
    58921 taaaaagaaa aaggctgacg atttctcgtc agcctttgct atatctggtg gcccctgctg
    58981 gacttgaacc agcgaccaag cgattatgag tcgcctgctc taaccactga gctaaggggc
    59041 catggtagcg gattataaag taactccgtg tcgcaatcca gccattaccg cgcgcctgct
    59101 gtttttataa acaatgtatt ttcaatccgt tatacttttc ctggtgatgt tagaaaggag
    59161 taaatatggt tgaggatatt ttggctccag ggttacgggt cgtgttttgc ggtatcaacc
    59221 ctgggctttc atccgccggg actggttttc cctttgctca tccggcaaat cgcttctgga
    59281 aggtgatata tcaggccggg tttaccgacc gtcagttgaa gccgcaggag gcacagcatc
    59341 tgctggatta tcgttgtggc gtcaccaaac tggtagaccg tccaacggtg caagccaatg
    59401 aagtttcaaa gcaggagctg cacgcaggtg ggcgtaagct gattgaaaaa attgaagatt
    59461 atcagccgca ggcgttggcg attctgggca aacaagcata tgaacaggga ttcagccagc
    59521 gcggtgcaca gtgggggaaa caaacgctca ccattggttc gacgcagatt tgggtgctgc
    59581 caaatcccag cggtttaagt cgcgtttcac tggagaaact ggttgaagcg tatcgcgagc
    59641 tggaccaggc gctggtagtg cgtgggcgat aaaaaacgcc accga
//
LOCUS       DADLXX010000010        59523 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLXX010000010 DADLXX010000000
VERSION     DADLXX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.010"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNX27_000597"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNX27_000597"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7881916.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNX27_000598"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNX27_000598"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7881917.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNX27_000599"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNX27_000599"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7881918.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNX27_000600"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNX27_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7881919.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNX27_000601"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNX27_000601"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7881920.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNX27_000602"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNX27_000602"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7881921.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JNX27_000603"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JNX27_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7881922.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWCGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNX27_000604"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNX27_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7881923.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEMAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNX27_000605"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNX27_000605"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7881924.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNX27_000606"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNX27_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7881925.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JNX27_000607"
     CDS             complement(14847..15542)
                     /locus_tag="JNX27_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7881926.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JNX27_000608"
     CDS             complement(15665..16702)
                     /locus_tag="JNX27_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7881927.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="JNX27_000609"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="JNX27_000609"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7881928.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNX27_000610"
     CDS             complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNX27_000610"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7881929.1"
                     /translation="MLLCSQEVVLAPPVSYGVEEDVFHPVRAKQGMVASVDATATQVG
                     VDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKNGNTTAIDFREMAPAK
                     ATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGTMPLNKVVQPAFKLAR
                     DGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKSGTLVQANLAKSLEMIAE
                     NGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPISGDYRGYQVYSMPPPS
                     SGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPDFVKVPWQ
                     ALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNAVAVTYTL
                     NTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANAVGPNKRPLSSMSPTI
                     VVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNAPRFHHQWLPDELRVE
                     KGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGVSDPRSVDDLTAGY"
     gene            19423..19863
                     /locus_tag="JNX27_000611"
     CDS             19423..19863
                     /locus_tag="JNX27_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7881930.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNX27_000612"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNX27_000612"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7881931.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNX27_000613"
     CDS             complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNX27_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7881932.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNX27_000614"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNX27_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7881933.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNX27_000615"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNX27_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY7881934.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNX27_000616"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNX27_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY7881935.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNX27_000617"
     CDS             complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNX27_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7881936.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNX27_000618"
     CDS             complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNX27_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7881937.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26601..27878)
                     /locus_tag="JNX27_000619"
     CDS             complement(26601..27878)
                     /locus_tag="JNX27_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7881938.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNX27_000620"
     CDS             complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNX27_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7881939.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNX27_000621"
     CDS             complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNX27_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7881940.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLACSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30382..30765
                     /gene="panM"
                     /locus_tag="JNX27_000622"
     CDS             30382..30765
                     /gene="panM"
                     /locus_tag="JNX27_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7881941.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30964..32067)
                     /locus_tag="JNX27_000623"
     CDS             complement(30964..32067)
                     /locus_tag="JNX27_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7881942.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNX27_000624"
     CDS             complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNX27_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7881943.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNX27_000625"
     CDS             complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNX27_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7881944.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNX27_000626"
     CDS             complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNX27_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7881945.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNX27_000627"
     CDS             complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNX27_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7881946.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36805..37401
                     /gene="rsmD"
                     /locus_tag="JNX27_000628"
     CDS             36805..37401
                     /gene="rsmD"
                     /locus_tag="JNX27_000628"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7881947.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37391..37660
                     /locus_tag="JNX27_000629"
     CDS             37391..37660
                     /locus_tag="JNX27_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7881948.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37663..38022)
                     /locus_tag="JNX27_000630"
     CDS             complement(37663..38022)
                     /locus_tag="JNX27_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7881949.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38163..38789
                     /locus_tag="JNX27_000631"
     CDS             38163..38789
                     /locus_tag="JNX27_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7881950.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38863..41061
                     /gene="zntA"
                     /locus_tag="JNX27_000632"
     CDS             38863..41061
                     /gene="zntA"
                     /locus_tag="JNX27_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY7881951.1"
                     /translation="MSTPDNHGKKAPQFAAFKPITTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNX27_000633"
     CDS             complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNX27_000633"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7881952.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41629..42294
                     /locus_tag="JNX27_000634"
     CDS             41629..42294
                     /locus_tag="JNX27_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7881953.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42367..42924
                     /gene="dcrB"
                     /locus_tag="JNX27_000635"
     CDS             42367..42924
                     /gene="dcrB"
                     /locus_tag="JNX27_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7881954.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42928..44145)
                     /locus_tag="JNX27_000636"
                     /pseudo
     CDS             complement(42928..44145)
                     /locus_tag="JNX27_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44277..45326
                     /locus_tag="JNX27_000637"
     CDS             44277..45326
                     /locus_tag="JNX27_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7881955.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLYLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45381..45968
                     /gene="acpT"
                     /locus_tag="JNX27_000638"
     CDS             45381..45968
                     /gene="acpT"
                     /locus_tag="JNX27_000638"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7881956.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46079..47653
                     /gene="nikA"
                     /locus_tag="JNX27_000639"
     CDS             46079..47653
                     /gene="nikA"
                     /locus_tag="JNX27_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7881957.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47653..48597
                     /gene="nikB"
                     /locus_tag="JNX27_000640"
     CDS             47653..48597
                     /gene="nikB"
                     /locus_tag="JNX27_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7881958.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPTTL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48594..49427
                     /gene="nikC"
                     /locus_tag="JNX27_000641"
     CDS             48594..49427
                     /gene="nikC"
                     /locus_tag="JNX27_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7881959.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49427..50191
                     /gene="nikD"
                     /locus_tag="JNX27_000642"
     CDS             49427..50191
                     /gene="nikD"
                     /locus_tag="JNX27_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7881960.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50188..50994
                     /gene="nikE"
                     /locus_tag="JNX27_000643"
     CDS             50188..50994
                     /gene="nikE"
                     /locus_tag="JNX27_000643"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7881961.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51000..51401
                     /gene="nikR"
                     /locus_tag="JNX27_000644"
     CDS             51000..51401
                     /gene="nikR"
                     /locus_tag="JNX27_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7881962.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51521..51880)
                     /locus_tag="JNX27_000645"
     CDS             complement(51521..51880)
                     /locus_tag="JNX27_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7881963.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51877..52152)
                     /locus_tag="JNX27_000646"
     CDS             complement(51877..52152)
                     /locus_tag="JNX27_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7881964.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52211..53335)
                     /locus_tag="JNX27_000647"
     CDS             complement(52211..53335)
                     /locus_tag="JNX27_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7881965.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNX27_000648"
     CDS             complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNX27_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7881966.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQLTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56067..57134)
                     /locus_tag="JNX27_000649"
     CDS             complement(56067..57134)
                     /locus_tag="JNX27_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7881967.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57500..59122)
                     /locus_tag="JNX27_000650"
     CDS             complement(57500..59122)
                     /locus_tag="JNX27_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7881968.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKSQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINLGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        14066 a        16425 c        15074 g        13958 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggtgcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 tttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgaa cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatacgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25381 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25441 acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25501 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25561 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25621 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25681 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25741 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25801 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25861 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25921 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25981 caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    26041 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    26101 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    26161 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26221 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26281 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26341 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26401 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26461 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26521 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26581 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26641 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26701 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26761 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26821 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26881 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc
    26941 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    27001 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    27061 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    27121 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27181 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27241 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27301 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27361 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27421 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27481 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27541 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27601 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27661 gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27721 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27781 gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27841 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27901 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27961 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    28021 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    28081 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    28141 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28201 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28261 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28321 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28381 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28441 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28501 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28561 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28621 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28681 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28741 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28801 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28861 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28921 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28981 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    29041 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    29101 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    29161 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29221 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29281 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29341 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29401 tttcagcccg tcctgcaccg aacacgccag cccttcgcca tactgttgtt tgtcgtgaat
    29461 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29521 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29581 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29641 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29701 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29761 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29821 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29881 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29941 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    30001 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    30061 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    30121 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30181 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30241 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30301 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30361 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30421 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30481 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30541 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30601 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30661 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30721 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30781 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30841 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30901 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30961 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    31021 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    31081 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    31141 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31201 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31261 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31321 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31381 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31441 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31501 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31561 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31621 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31681 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31741 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31801 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31861 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31921 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31981 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    32041 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    32101 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag
    32161 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32221 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32281 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32341 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32401 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32461 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32521 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32581 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32641 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32701 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32761 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32821 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32881 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32941 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    33001 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    33061 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    33121 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33181 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33241 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33301 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33361 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33421 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33481 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33541 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33601 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33661 gcagcgcacc accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33721 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33781 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33841 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaatgccgt
    33901 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33961 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    34021 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    34081 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    34141 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34201 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34261 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34321 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34381 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34441 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34501 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34561 aggttaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34621 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34681 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34741 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34801 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34861 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34921 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34981 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    35041 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    35101 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    35161 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35221 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35281 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35341 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35401 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35461 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35521 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35581 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35641 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35701 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35761 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35821 tctcacccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35881 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35941 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    36001 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    36061 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct
    36121 gttcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36181 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36241 cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt
    36301 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36361 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36421 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36481 gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa
    36541 cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct
    36601 ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg
    36661 actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat agcagctaaa
    36721 aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca agtatgtgtt
    36781 atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc
    36841 cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg
    36901 cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt
    36961 gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg
    37021 cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt
    37081 aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca
    37141 ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc gttccgccgt
    37201 ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc
    37261 ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca
    37321 ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa
    37381 ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt
    37441 aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct
    37501 gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa
    37561 agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga
    37621 actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa
    37681 gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccacgg tcagggcgtg
    37741 gtactgctgg gcgtcgagac gaaacgtctg ctccattcct ccgctttgcg gtttgaagct
    37801 tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc
    37861 gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat
    37921 atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat
    37981 taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa
    38041 aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgctact
    38101 aaactggaag caagaccgta ggcattccgc ttacgaaaaa ataacgaatt caaggaacta
    38161 agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt
    38221 atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt
    38281 tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt
    38341 gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca
    38401 tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc
    38461 tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga
    38521 ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc
    38581 tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct
    38641 ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact
    38701 atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact
    38761 tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt
    38821 gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat
    38881 cacggcaaga aagcccctca atttgctgcg ttcaaaccga taaccacggt acagaacgcc
    38941 aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc
    39001 ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta
    39061 gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa
    39121 aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa
    39181 gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa
    39241 gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag
    39301 ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg
    39361 attgctcatc aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta
    39421 atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg
    39481 ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag
    39541 ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag
    39601 cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt
    39661 gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc
    39721 ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc
    39781 accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt
    39841 gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt
    39901 atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg
    39961 ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg
    40021 ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc
    40081 gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa
    40141 ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg
    40201 cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg
    40261 gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag
    40321 gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt
    40381 gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat
    40441 gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta
    40501 cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca
    40561 actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat
    40621 ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg
    40681 ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc
    40741 ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt
    40801 agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc
    40861 ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc
    40921 accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg
    40981 ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta
    41041 aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc
    41101 gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc
    41161 tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc
    41221 agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg
    41281 gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc
    41341 accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga
    41401 tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt
    41461 aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt
    41521 atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc
    41581 gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc
    41641 tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct tctggtgatc
    41701 acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca taccacctgg
    41761 ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc
    41821 gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac
    41881 gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc
    41941 aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc
    42001 ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca
    42061 gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg
    42121 cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt
    42181 ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg
    42241 ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc
    42301 gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag
    42361 aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg
    42421 gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggtcgcgga aagtaacgct
    42481 accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg
    42541 accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc
    42601 accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg
    42661 ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc
    42721 aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg
    42781 aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc
    42841 atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc
    42901 attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc
    42961 cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc
    43021 cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt
    43081 cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc cctaattttg
    43141 ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc
    43201 ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag
    43261 caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt
    43321 aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc
    43381 gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac
    43441 gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac
    43501 cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa
    43561 caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca
    43621 gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc
    43681 gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt
    43741 cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa
    43801 taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct
    43861 caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta
    43921 acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc
    43981 tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg
    44041 caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt
    44101 caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt
    44161 tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga
    44221 atattgctgg cgtgactccc gctacactta acacaaaaag tcacaaggaa gccccaatgg
    44281 aaacccctca acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag
    44341 tgatcctcgc gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt
    44401 tttttgccat cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc
    44461 ccgttgccat tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg
    44521 tactggcggc atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga
    44581 cgcgcaaact ttttaaattg caggagatgt tgccttttct ttatttgcat atgtcgccgg
    44641 agcgaatgct gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga
    44701 ccgggctttc cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc
    44761 tgtttgaagt gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc
    44821 acatcgcagg attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc
    44881 tactcagttt atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt
    44941 ttgcgctgat gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg
    45001 taatttccgc cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta
    45061 ttctggtcgg cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac
    45121 ggatgatggg ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct
    45181 ggggatggct gctcggcccg gtagggatgc tactttcggt gccattaacc agagtgtgta
    45241 aaatctggat ggaaacgacg aaaggtggca gcaaactggc gattttactg gggccggaca
    45301 gaccgaaaag tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc
    45361 cattaaacgt tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg
    45421 cagctccact gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct
    45481 ggctggcggg gcgtgcattg ctttcgcaca cgctttcccc gctaccggag atcatctatg
    45541 gcgaacaagg caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata
    45601 gcggtgacga tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag
    45661 tgattcgccc gcgcgccaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac
    45721 acgctgaaat ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga
    45781 cgcgcaaaga agccatcgtt aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag
    45841 atagcaccta tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg
    45901 cgatctgcac ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag
    45961 ttaactgatc cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac
    46021 gaatacttaa aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat
    46081 gctctccaca ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca
    46141 tgccgctgca ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc
    46201 gcacctttac acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa
    46261 atatcaggca gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga
    46321 tggtaaaacc tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt
    46381 cgatgccgag gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc
    46441 ctggctggag ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca
    46501 aattaccctg aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt
    46561 ccgttttatc gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc
    46621 gattggcacc ggaccgtgga ttttgcagga atcgaaactg aatcagtacg atgtcttcgt
    46681 ccgtaacgaa aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat
    46741 cccggacccg actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg
    46801 aaacgaaggg ttattaccgc tcgatacctt cgcccgcttt agccaaaatc cggcttacca
    46861 cacccaactg tcacagccgc tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc
    46921 caccaacgag ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat
    46981 tgataacgcg ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc
    47041 ttacgctaat ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct
    47101 ggaaaaagcc ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc
    47161 gctgcgcatt gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat
    47221 cattcaggct gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag
    47281 cagtatctat gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg
    47341 cgcgccatac gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt
    47401 ccaggcacag caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct
    47461 ggcgacccat gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca
    47521 tgacgaggcg gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga
    47581 gctgggtaat atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc
    47641 ggtgaaacct taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg
    47701 ctcgccgcct cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc
    47761 gattatctgc gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc
    47821 atgctcgggc tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg
    47881 catcttgact ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac
    47941 ttcctgccca ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc
    48001 gtaccgctcg gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc
    48061 tttatcgcgt ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg
    48121 gcgttttcgg tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc
    48181 attttacctg cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc
    48241 gccagtatgc tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg
    48301 aatgacaaac agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc
    48361 gccgtgggga tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt
    48421 gcctggcctg gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg
    48481 attcagtgct ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat
    48541 ttgctcaacg ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact
    48601 ttttcctctc ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc
    48661 tgattgcgct caccagccag tggtggctgc cgtacgatcc acaggcaatt gatttgccgt
    48721 cgtgcctgct ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata
    48781 ttttctcgcg gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc
    48841 tgctggtgct gacattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcgcg
    48901 ttgatcaggc caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt
    48961 cgttctttat ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc
    49021 tgtcgcactg ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac
    49081 gcgagtttgt gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc
    49141 atctggcagg cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata
    49201 tgatgctgca cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg
    49261 aatggggcgt gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt
    49321 tctggccggg gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac
    49381 tgcgcgatca tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat
    49441 tgaactacgt aatatcgcgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac
    49501 cctgcaacgc gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac
    49561 ctgcgccgcg acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt
    49621 agccgatggc aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat
    49681 gcagaacccg cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac
    49741 ctgcctggcg ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt
    49801 ggggctggaa aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat
    49861 gttgcagcgc atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga
    49921 tgaaccgacc accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag
    49981 cattatgcaa aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc
    50041 gcgtctggcg gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt
    50101 agaaacgctg tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct
    50161 cgccctctac ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat
    50221 cactatgccc acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt
    50281 tccctgaccc tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa
    50341 agcaccctcg cgcggctgct ggtaggctta gagtctccct cacaagggaa tattagctgg
    50401 cgcggcgaat cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt
    50461 cagatggtgt ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc
    50521 ctgcgtgaac cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc
    50581 agcgaaatgc tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag
    50641 ttaagcggcg gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa
    50701 ttgctgattc tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt
    50761 cgcctgctga aaaatctaca acaacagttt ggcactgcct gcctgttcat cactcatgac
    50821 ttacgcctgg tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc
    50881 gaaacccagg cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa
    50941 aacgcggtat tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa
    51001 tgcaacgagt caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc
    51061 agcgtcgtgg ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg
    51121 cgcaggaagc tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg
    51181 aacacgaaaa acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc
    51241 tctccgtcgc cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt
    51301 tgaaaggtga catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg
    51361 tgcggcacgg gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga
    51421 aagaaaagat attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc
    51481 tgtaaaggca gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct
    51541 cattcagtgt ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat
    51601 taaggcgttc actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac
    51661 gcgcatacgg ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct
    51721 ctccttcttt ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc
    51781 ccaggaattt gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc
    51841 cggcaatctc catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag
    51901 taaccagtca cgcacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg
    51961 atggaaacac gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt
    52021 aatttctccg cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg
    52081 aacaggtgtt ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac
    52141 cttggttctc atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag
    52201 tttcccttcc ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga
    52261 aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg
    52321 caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca
    52381 taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt
    52441 gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg
    52501 tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa
    52561 acagcgggat cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg
    52621 ataatcccga taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct
    52681 caaacggcgt tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga
    52741 tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca
    52801 ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata
    52861 ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa
    52921 tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca
    52981 cctgaatatc cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta
    53041 tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct
    53101 ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt
    53161 gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg
    53221 acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga
    53281 gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg cgcatcatcc
    53341 ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa
    53401 cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc
    53461 gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag
    53521 cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc
    53581 cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc
    53641 agtggcaatg atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg
    53701 cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag
    53761 cgcgatgtac ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta
    53821 aaggttgata atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga
    53881 cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac
    53941 gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt
    54001 caattgtcgg ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt
    54061 taccgtttcg gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt
    54121 accacgcgcg atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc
    54181 cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta
    54241 acgggaaccg gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag
    54301 caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag
    54361 gatcaccgtt cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc
    54421 ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt
    54481 ggtgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa
    54541 ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg
    54601 tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc
    54661 ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac
    54721 catcaactgc cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag
    54781 gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc
    54841 gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat
    54901 ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc
    54961 caggttttgc cgcacggtga gttcgttata gagcgaaaac gactgcgaca tatagcccac
    55021 acgacggcgg gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc
    55081 ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt
    55141 cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc
    55201 aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc
    55261 aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag
    55321 attaataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct
    55381 gccggttgcc agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc
    55441 ttccatatag gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc
    55501 gatcagatcc cagaactggg cgcgggagag cgggtcaacc cccgttgttg gctcatcaag
    55561 gatcaacagt tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc
    55621 gccggagagt ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag
    55681 ctcattaatt cgcacttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc
    55741 gacattttca tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca
    55801 ggcgatgcgc gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac
    55861 caccacattg ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt
    55921 cccgacgcca tccggaccaa tcagcccgac catgcaacgg gccggaatat cgagagtgat
    55981 attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg
    56041 cgggacagga accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc
    56101 cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat
    56161 tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc
    56221 agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa
    56281 ctgatggttg caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg
    56341 cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg
    56401 agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc
    56461 cgatactgca cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca
    56521 atgcgccgtt cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg
    56581 cgtgctgctt ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca
    56641 gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatcgcccct
    56701 cgttgggcga gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg
    56761 cgttgattga ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc
    56821 tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt
    56881 tcctgcaaca cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct
    56941 tctttcacca gaatggtgtc gatacgcccg gcagttttgc tggcgatatc cacttccgtc
    57001 gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc
    57061 caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc
    57121 ttactcttat ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac
    57181 tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac
    57241 gaccagcgcg cagagcattg agcgccaacg gggaaggctt agcgaaaacg ggtagtaatg
    57301 ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat
    57361 aaaactggct gacaaaacgg tatccatcct gttggatcgc gtttcacgcc tgttcagttg
    57421 caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa
    57481 tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa
    57541 gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg
    57601 caccaaggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac
    57661 gggcgttaag acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt
    57721 tatgtgcaac aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt
    57781 taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc
    57841 ggctagtttt aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg
    57901 ctaattcgac cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta
    57961 taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt
    58021 atttggggtc atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa
    58081 gtggtttcat gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc
    58141 cagctaatac atgaacattt tcattgatac tcattatgcg ttttaacaaa tcgatgataa
    58201 attgaacgcc acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg
    58261 gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc
    58321 ttctgttcag tatactgatt attgtattaa cattagtgtc aagttgctca ttaaagttct
    58381 cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt
    58441 ctccttcagc catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga
    58501 ctgagctatc ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac
    58561 aattggagaa ataggttctg tcagcgaact gggattttat aattgtttcg aatttaatat
    58621 caaatacatt gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg
    58681 caacatcatt agcattatca tttactgtca tattattaag taaataaaca atgctgcacc
    58741 aattaatatc ggtattatca agagggcaac cgtgattttt actattaatt gtcacaataa
    58801 actgataaca gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt
    58861 tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga
    58921 aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa
    58981 tacttgatat atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt
    59041 tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc
    59101 ctgccacagt tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt
    59161 taacatttta tcttatgaca tacacctcat attcgatggg aaaataatta tattttgcac
    59221 aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct
    59281 aaagattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa acctatgaaa
    59341 tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac
    59401 aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat
    59461 ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc
    59521 caa
//
LOCUS       DADLXX010000011        59445 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLXX010000011 DADLXX010000000
VERSION     DADLXX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.011"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            545..1834
                     /locus_tag="JNX27_000651"
     CDS             545..1834
                     /locus_tag="JNX27_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7881969.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLSMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="JNX27_000652"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="JNX27_000652"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7881970.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3280..4125
                     /gene="xni"
                     /locus_tag="JNX27_000653"
     CDS             3280..4125
                     /gene="xni"
                     /locus_tag="JNX27_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7881971.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTLAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="JNX27_000654"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="JNX27_000654"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7881972.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="JNX27_000655"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="JNX27_000655"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7881973.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="JNX27_000656"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="JNX27_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY7881974.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="JNX27_000657"
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="JNX27_000657"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY7881975.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="JNX27_000658"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="JNX27_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="JNX27_000659"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="JNX27_000659"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7881976.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="JNX27_000660"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="JNX27_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7881977.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="JNX27_000661"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="JNX27_000661"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7881978.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="JNX27_000662"
     CDS             complement(13550..13945)
                     /locus_tag="JNX27_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7881979.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /locus_tag="JNX27_000663"
     CDS             complement(13964..14881)
                     /locus_tag="JNX27_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7881980.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="JNX27_000664"
     CDS             complement(15232..15459)
                     /locus_tag="JNX27_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7881981.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="JNX27_000665"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="JNX27_000665"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7881982.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="JNX27_000666"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="JNX27_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7881983.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELELRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="JNX27_000667"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="JNX27_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7881984.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="JNX27_000668"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="JNX27_000668"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7881985.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19700..19776
                     /locus_tag="JNX27_000669"
     tRNA            19700..19776
                     /locus_tag="JNX27_000669"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19734..19736,aa:Met,seq:cat)
     gene            19810..19886
                     /locus_tag="JNX27_000670"
     tRNA            19810..19886
                     /locus_tag="JNX27_000670"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19844..19846,aa:Met,seq:cat)
     gene            complement(19961..21214)
                     /locus_tag="JNX27_000671"
     CDS             complement(19961..21214)
                     /locus_tag="JNX27_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7881986.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21446..22777
                     /gene="argA"
                     /locus_tag="JNX27_000672"
     CDS             21446..22777
                     /gene="argA"
                     /locus_tag="JNX27_000672"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7881987.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22839..24665)
                     /gene="recD"
                     /locus_tag="JNX27_000673"
     CDS             complement(22839..24665)
                     /gene="recD"
                     /locus_tag="JNX27_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY7881988.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMLL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24665..28207)
                     /gene="recB"
                     /locus_tag="JNX27_000674"
     CDS             complement(24665..28207)
                     /gene="recB"
                     /locus_tag="JNX27_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7881989.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="JNX27_000675"
     CDS             complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="JNX27_000675"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7881990.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31264..34632)
                     /gene="recC"
                     /locus_tag="JNX27_000676"
     CDS             complement(31264..34632)
                     /gene="recC"
                     /locus_tag="JNX27_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7881991.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPLWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34645..34968)
                     /locus_tag="JNX27_000677"
     CDS             complement(34645..34968)
                     /locus_tag="JNX27_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7881992.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34953..35360)
                     /locus_tag="JNX27_000678"
     CDS             complement(34953..35360)
                     /locus_tag="JNX27_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7881993.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35357..35920)
                     /locus_tag="JNX27_000679"
     CDS             complement(35357..35920)
                     /locus_tag="JNX27_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7881994.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35911..36381)
                     /locus_tag="JNX27_000680"
                     /pseudo
     CDS             complement(35911..36381)
                     /locus_tag="JNX27_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="JNX27_000681"
     CDS             complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="JNX27_000681"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7881995.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="JNX27_000682"
     CDS             complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="JNX27_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7881996.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="JNX27_000683"
     CDS             complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="JNX27_000683"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7881997.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="JNX27_000684"
     CDS             complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="JNX27_000684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7881998.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41866..42555
                     /gene="mutH"
                     /locus_tag="JNX27_000685"
     CDS             41866..42555
                     /gene="mutH"
                     /locus_tag="JNX27_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7881999.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42624..43337
                     /locus_tag="JNX27_000686"
     CDS             42624..43337
                     /locus_tag="JNX27_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7882000.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43475..43693
                     /gene="ygdR"
                     /locus_tag="JNX27_000687"
     CDS             43475..43693
                     /gene="ygdR"
                     /locus_tag="JNX27_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7882001.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43801..44841
                     /locus_tag="JNX27_000688"
     CDS             43801..44841
                     /locus_tag="JNX27_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7882002.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44874..46067)
                     /gene="lplT"
                     /locus_tag="JNX27_000689"
     CDS             complement(44874..46067)
                     /gene="lplT"
                     /locus_tag="JNX27_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7882003.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FCPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACVLAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(46060..48219)
                     /gene="aas"
                     /locus_tag="JNX27_000690"
     CDS             complement(46060..48219)
                     /gene="aas"
                     /locus_tag="JNX27_000690"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7882004.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48608..49639
                     /gene="galR"
                     /locus_tag="JNX27_000691"
     CDS             48608..49639
                     /gene="galR"
                     /locus_tag="JNX27_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7882005.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49646..50908)
                     /gene="lysA"
                     /locus_tag="JNX27_000692"
     CDS             complement(49646..50908)
                     /gene="lysA"
                     /locus_tag="JNX27_000692"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7882006.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51030..51965
                     /gene="lysR"
                     /locus_tag="JNX27_000693"
     CDS             51030..51965
                     /gene="lysR"
                     /locus_tag="JNX27_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7882007.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51952..52644)
                     /gene="ygeA"
                     /locus_tag="JNX27_000694"
     CDS             complement(51952..52644)
                     /gene="ygeA"
                     /locus_tag="JNX27_000694"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7882008.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52773..54186)
                     /locus_tag="JNX27_000695"
                     /pseudo
     CDS             complement(52773..54186)
                     /locus_tag="JNX27_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(54501..55262)
                     /gene="kduD"
                     /locus_tag="JNX27_000696"
     CDS             complement(54501..55262)
                     /gene="kduD"
                     /locus_tag="JNX27_000696"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7882009.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55292..56128)
                     /gene="kduI"
                     /locus_tag="JNX27_000697"
     CDS             complement(55292..56128)
                     /gene="kduI"
                     /locus_tag="JNX27_000697"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7882010.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(56448..57596)
                     /locus_tag="JNX27_000698"
     CDS             complement(56448..57596)
                     /locus_tag="JNX27_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY7882011.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            57851..58009
                     /locus_tag="JNX27_000699"
     CDS             57851..58009
                     /locus_tag="JNX27_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter"
                     /protein_id="HAY7882012.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTY"
     gene            58166..59080
                     /locus_tag="JNX27_000700"
     CDS             58166..59080
                     /locus_tag="JNX27_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7882013.1"
                     /translation="MAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHAT
                     IRVMGFLVFPLIAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSH
                     TPIISTFAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAK
                     EEGVTILSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQV
                     GVKKSRAFNHALSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLI
                     PALKPWRSIGNLITLIVGILCVSVMFFS"
BASE COUNT        14657 a        15972 c        14485 g        14331 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgcc
      121 atacaattta tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac agcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg agtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg ggctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atctccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacactggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcgacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcttgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattccaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtatcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatacc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg cagcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctgggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatagccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 ctccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca actaaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggtatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccgggatct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaagcgatt
    15481 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg gcggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcga
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tggaattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aggttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcaccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgttttgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaataagc ttagtggttt gtttttgttc
    19621 aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag
    19681 tatagtgcgc atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc
    19741 cgaaggtcgt cggttcaaat ccggcccccg caaccaatca aatttgatga agtaaaagca
    19801 gtacggtgac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    19861 cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
    19921 ctgatctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    19981 ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
    20041 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    20101 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
    20161 ttttatgcag cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc
    20221 tgtcagcaat agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    20281 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
    20341 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac
    20401 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
    20461 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    20521 tattgccctc tttctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
    20581 tttcgcgcgt tttgtatttc cccaccgcac cggaatcttc gccaccgtgg ccagggtcaa
    20641 gcataatgac aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg
    20701 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    20761 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    20821 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
    20881 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    20941 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    21001 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    21061 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    21121 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac
    21181 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    21241 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    21301 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    21361 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    21421 aaataatcat gcaaagaggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
    21481 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    21541 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    21601 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    21661 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    21721 aacactagaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    21781 gtcaatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa
    21841 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    21901 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gaaagcggtg caatagtgct
    21961 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    22021 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
    22081 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
    22141 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    22201 tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga
    22261 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    22321 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    22381 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    22441 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    22501 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    22561 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
    22621 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    22681 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    22741 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg
    22801 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    22861 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    22921 tcggcataca gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    22981 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    23041 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    23101 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    23161 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
    23221 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    23281 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    23341 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    23401 agcagatcca gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct
    23461 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    23521 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    23581 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    23641 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
    23701 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    23761 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc
    23821 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    23881 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
    23941 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    24001 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    24061 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
    24121 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    24181 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    24241 tccagggttt gcgccagtag agcttcatca acctcaatgg tatgattcac ctcattgaag
    24301 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    24361 aggagcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    24421 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
    24481 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    24541 aacagtgccg ccgcgagggt gacagcagga tgttcatctc ccgccacggt cagggcaaat
    24601 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    24661 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    24721 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    24781 ggaacagata gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc
    24841 gcagataacg atgcagtgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    24901 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    24961 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    25021 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    25081 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    25141 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    25201 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    25261 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    25321 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    25381 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    25441 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    25501 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    25561 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    25621 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    25681 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    25741 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    25801 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    25861 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    25921 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    25981 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    26041 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    26101 tttccagctg cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac
    26161 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    26221 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    26281 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    26341 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    26401 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    26461 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    26521 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgt
    26581 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    26641 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    26701 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    26761 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
    26821 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    26881 aagtgtagtg ggcgtgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    26941 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    27001 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    27061 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    27121 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    27181 tttcacgcgc tactgtttcg cggatctcag ccaatgctcg ggtgatcacc agatcgcgga
    27241 tcgacaatgg ttctgcaagc agttgatcga tcacctcaaa cagtggatgt cgcggggttt
    27301 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    27361 gcaactgata actgtttgtc tcttcttctg cccaagcgct gatcttttcg atccatttag
    27421 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    27481 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    27541 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
    27601 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    27661 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    27721 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    27781 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
    27841 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    27901 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    27961 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    28021 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    28081 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    28141 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    28201 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    28261 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    28321 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    28381 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    28441 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    28501 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    28561 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    28621 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    28681 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    28741 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    28801 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    28861 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    28921 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    28981 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    29041 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    29101 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    29161 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
    29221 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    29281 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    29341 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    29401 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    29461 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    29521 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
    29581 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    29641 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    29701 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    29761 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    29821 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    29881 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    29941 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    30001 gccaggcctt tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    30061 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    30121 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    30181 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    30241 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    30301 gtttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    30361 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    30421 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    30481 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcactgacc
    30541 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    30601 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
    30661 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    30721 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    30781 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    30841 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
    30901 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    30961 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    31021 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
    31081 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
    31141 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    31201 tcagtccgtg actggtgcgc atcataaagt aagcggatag attgcgcaat ttttatacaa
    31261 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    31321 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    31381 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    31441 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    31501 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    31561 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    31621 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
    31681 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    31741 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    31801 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    31861 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    31921 tgccgcccgg aagcggcgga acaagcgttc agcatcatcc tgctcaacca gtgcattcaa
    31981 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
    32041 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    32101 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    32161 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    32221 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    32281 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    32341 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    32401 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    32461 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    32521 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    32581 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    32641 cagagtacaa atattaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    32701 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    32761 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    32821 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    32881 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    32941 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    33001 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    33061 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    33121 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    33181 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
    33241 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    33301 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    33361 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    33421 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
    33481 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
    33541 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
    33601 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
    33661 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    33721 cccccatgag gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    33781 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    33841 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    33901 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    33961 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    34021 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    34081 ccagagcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg
    34141 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    34201 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    34261 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    34321 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    34381 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    34441 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    34501 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    34561 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    34621 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    34681 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    34741 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    34801 ctgccaacca tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa
    34861 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat
    34921 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa tgccccctct
    34981 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatttttcc atcaatgact
    35041 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    35101 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    35161 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    35221 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    35281 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    35341 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    35401 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    35461 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    35521 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    35581 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccgttac
    35641 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    35701 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    35761 gcagccagat ttcatcttcc agtgccagct tacgagtgct cgttaaactt tcacgctgta
    35821 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    35881 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    35941 ataaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    36001 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    36061 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    36121 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    36181 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    36241 ccgcgcctag ctggcggttt gccaaagccg ttgcgactgc tgccagtatt gccagccata
    36301 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    36361 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    36421 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    36481 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaccgacgc acactggcgt
    36541 cggctctggc agtatgtttc gtaattagat agccaccggc gctttaatgc ccggatgtgg
    36601 atcgtagcct tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg
    36661 tttgataatc aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc
    36721 catatggttg ctgtacaggt gcgtatcgcc accggtccag acaaaatcac ccacttccag
    36781 atcgcactgc tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag
    36841 gccgaggaag acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac
    36901 atagaactgg aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt
    36961 ccacgctgaa acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt
    37021 agtaatctgg tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata
    37081 cactggcccg aggtcgccat tttcatcggc ccattcgtcc cagatggtga cattgttttc
    37141 gtgtagataa gcaatgttag tatcgccctg caggaaccac agcagttcgt ggatgatgga
    37201 acgcaggtgg caacgtttgg ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat
    37261 ctgatgacca aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc
    37321 gtcgagcact ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg
    37381 ctgtgggctg cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat
    37441 ggaaagaatt tgccccatgc tgatgtactg cacccaggca ccggtaaact gcgcgtcggg
    37501 ctggcggaaa aactcaacaa tgatgcgaaa cgcgccgtag ccaatcagga acaagcctga
    37561 gacagctccc attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac
    37621 accttccagc agcagttcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc
    37681 gaaaatggat tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc
    37741 tgggaacagc atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat
    37801 aaagttgccc agacgcccgg caccaagacc aaacggaatg agtggtgcga taaaatcaga
    37861 gacctggaag aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc
    37921 aatcaggccg ccgtggaaag acatgccgcc gtcccagaca cggaacagat acagcggatc
    37981 ggccataaac tgcgggaaat tgtagaacag aacataacca atacgtcccc cgaggaagac
    38041 gccgaggaag cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg
    38101 acgattcgcc cgacgtgttg ccagccacat tgcaaaaatg aaacccacca gatacatcag
    38161 gccgtaccag tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa
    38221 atgcagatag ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac
    38281 agcgattgaa atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag
    38341 gggacctggc tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc
    38401 tccgcgaatc agcccgccca tgccgcgacg ctccataaag gctgcaacct gatgacgaac
    38461 ttcggtcgcc agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc
    38521 aatgcgccgc agcaggtatt ttacccgcgc tacagaacgt ccgttcatag acagatggcg
    38581 atacccaagc ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa
    38641 acggagatcg ataccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat
    38701 tgcaggatga agactgtcgt aaatgttcgc cacccgggta ttgttgcgat caacggccag
    38761 aatgtattga gtcagatcgt tggtgccaac agagatgaaa tcgacccgct ttgccagatg
    38821 cggcagcata aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat
    38881 ttcgtaaccg atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc
    38941 gtcaacttca tcgaggctgg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc
    39001 attagcacgc agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat
    39061 gcgaatacca cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg
    39121 cttatctgct ccgacatcca gcgtacgcaa ggtgacgggt ttatcattga acatttgcag
    39181 catcccctga tactgcgcca cctgttcttc ttccgacggg aaaccacttt gcagcatgaa
    39241 tgggatttca gtgcgataaa gaccgatgcc atcaatacgg ctgcccagtt tttcttcatg
    39301 ttccgggctt aaaccagcat tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc
    39361 gggtaaattg acgtcatctt ccgccagacg gctaagctca atctcttcac taattagccg
    39421 ctgatattct tgcagcagta ccggctccgg atcgaccagc aattcaccgc gatagccatc
    39481 aacgatcagc gtcctacgat gcagcaccga aggctgaata tccgcgccca tcacggtagg
    39541 gatccccagc gcacgtacca tgatcgcagc atgggagttg gctgcgccat ctcgcacgac
    39601 aacaccgact aagcgatcct ggggcagctc agcaagagtt gtcgctgaca gttcatctgc
    39661 caccagaatg aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa
    39721 cagcaatcgc tgacccagcg cacgtaaatc gccagcccgc tctttgagat agttatcgct
    39781 tagcgcggca aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac
    39841 cgagccttta tcaacctcgg caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg
    39901 cgagtaaaga tcgaaaatag ccgccgtttc tttttgtgct ccggcggcaa agcgtttgct
    39961 gtagcggcga aactcgtttg ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag
    40021 agccggatcc agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg
    40081 ccagccttcg gcaatcgcca caccaggtgc tgccggtaat gcgcggattc gcgtctggcg
    40141 atattgccca aacagggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc
    40201 aagcgtcacc aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac
    40261 caccagtaca ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa
    40321 acgttcttct tttatggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag
    40381 gtttatcggt tccgccagcc tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt
    40441 tacagtgcga ccgcgtggtt ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg
    40501 atcatgatcg gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt
    40561 aaccagaata tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc
    40621 gcgcaggcga gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc
    40681 gttttgtggt tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat
    40741 taccctacgg tagacatcac gtttaaatga caccacctgt ctgaccggat accagtaact
    40801 tacccatcgc cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc
    40861 gccgcttacc agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt
    40921 gtcccaacgc accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag
    40981 gattcgaacg tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg
    41041 ctctgcggat tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa
    41101 tcgccgggcc cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg
    41161 gtagccatcg tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg
    41221 tttcatactc caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat
    41281 tgaataactc acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt
    41341 attcaccttt tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg
    41401 agcattctga ataagcccga tatagacatt ttaaaatatt cattattagt caatgcatta
    41461 catgtaattt gagtaatgaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg
    41521 ggttggcgaa agatcaacca atgccgtatt tatccacaga atgtgccact aagttaagca
    41581 ctgaaccact aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgaa aacagtagtg
    41641 gaggtttttc acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt
    41701 ttcagtgacc agatttggaa aacctattgc agtgttgcgc aactcgatta ccggcagcct
    41761 aaaaagcaat ataaatcaga gaattgaaca acacatgtgg aaaaagttac actgcgaata
    41821 ttcggcacat aattgctgtt tgttttttaa ccaaggcatc atgacatgtc ccaacctcgc
    41881 ccactgctct ctcctcccga aactgaagaa cagttgttag cgcaagcaca gcaactttct
    41941 ggttatacat tgggagaact ggcggcactt gccgggctgg ttacgccaga gaatttaaaa
    42001 cgcgataagg gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt
    42061 aaacctgagc aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt
    42121 cttggtcgtc cgctggaaac aacattcgtt tgtgttgccc cgttaacggg taatagcggg
    42181 gtgacctggg aaaccagcca tgtgcgccac aagctcaaac gcgtactgtg gataccggtt
    42241 gaaggcgagc gcagcatccc gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc
    42301 ccgaatgaag aggaagaccg acagctacgc gaagactggg aagaattaat ggatatgatt
    42361 gttctcggtc aggttgagcg gatcaccgct cgtcacgggg aatatttaca gataagaccg
    42421 aaagcagcga atgcgaaagc gcttaccgaa gccattggtg cccggggcga acggattctg
    42481 acgctgccac gcggctttta tttgaagaag aatttcacca gtgcactact ggcccgtcat
    42541 tttctgatcc agtagccatc gctttgacct gccgctttcc gggcatataa ttaccgcttc
    42601 atttttttgg cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcctggc
    42661 ttgcgctcgg tacgctgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc
    42721 tttctctggt ggtggcaaag cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt
    42781 tggcgggagc catggttatg cgtctggcgc tgctggcatc aatctcttgg gttacgcgcc
    42841 tgacaaatcc gctttttaca atattcagtc aggaaatttc tgcccgtgat ttgattctgc
    42901 ttctgggtgg cttgttcctt atctggaaag ccagcaagga aatccacgaa tccattgaag
    42961 gtgaagaaga agggctgaaa acacgcgttt catcattcct cggcgctatc gtgcagatta
    43021 tgctgctgga tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc
    43081 acctgtttat tatgatggca gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc
    43141 gctcgattgg tgattttgtc gaacgccatc cttcggtaaa aatgctggcg ctctctttcc
    43201 tgattctggt gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt
    43261 acatctactt cgcgatgttc ttctctattg cggttgaaag cctcaacttg attcgcaaca
    43321 aaaagaatcc gctctgatac tccgtacgct ctcctgcggg agggcgtact cactcctttt
    43381 accctcacag tttaagattt tcctgctttc aagatatata acgtcgattt ataaacagac
    43441 tattatcata ggtaggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc
    43501 tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa
    43561 agatggccgt atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt
    43621 gagttatcac gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat
    43681 tattgaacgt taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct
    43741 tttcccttcc ctctgccatt tttatattcc ttatgtcgtg attataaaaa ggaaacggct
    43801 atgcaatatc accgtatacc ccacagttcg ctggaagtca gcacgctggg gcttggcacg
    43861 atgacgtttg gtgaacagaa cagcgaagcc gacgcccacg cacaactcga ctatgccgtc
    43921 gctcagggca ttaaccttat cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa
    43981 acgcaagggt taaccgaaac ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa
    44041 aagttaatta tcgcctccaa agtgagcgga ccgtcgcgca ataatgacaa gggcatccgc
    44101 ccggatcagg cgctggatcg gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgc
    44161 ctacagactg attacctcga tctttatcag gtgcactggc cgcagcgccc gaccaactgc
    44221 ttcggcaaac tcggttatag ctggacggat tctgcgcctg cggtttcgct gctggatacg
    44281 ctggacgcac tggcagagta ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac
    44341 gaaactgcat ttggcgtaat gcgctacctg catctggcgg acaaacacga tctgccgcgt
    44401 attgtcacca ttcagaaccc ttacagtctg ttaaaccgca gttttgaagt aggtctggca
    44461 gaagtcagcc agtatgaagg ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg
    44521 ctgaccggga aatatctcaa tggtgcaaaa cccgctggcg cacgtaatac gctctttagt
    44581 cggttcaccc gctatagcgg tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc
    44641 gccagacgtc atggccttga tcctgctcag atggcgctcg cgtttgttcg ccgtcaaccg
    44701 tttgttgcca gcactctgct gggcgcaacc acgatggatc agctgaaaac taacatcgaa
    44761 agtttgcatc tggagttaag cgaagacgta ttagctgaaa ttgaagcggt acatcaggtt
    44821 tatacttatc ctgcaccata aagattaacg ccggttaaaa ccggcgttaa atattaatga
    44881 cggcgctgcc agatccacag cgccgttatt gccagcgcaa atagcgcacc gaagccaatg
    44941 ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc cagcatcaac
    45001 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    45061 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccagcacg
    45121 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga gaaaatcagt
    45181 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaacc
    45241 agcttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    45301 gtcggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    45361 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    45421 ccattgcacc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    45481 caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca
    45541 ccaccgtagg ccagtacgca tgcggccagg gcgaccagga catgccagtc agccagcaca
    45601 ccaccggcta cggaaccgag taaaatcgcc gctatggcag aagcttccat taaaccgtta
    45661 gcttttacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    45721 taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag
    45781 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    45841 aagctatccg ccacctgccc gacaaacggc gccaaaagaa tgtaagcacc tacaaacacc
    45901 atttgcagga tgggctggct ccactccgga cagaactgcg ctttcagtaa cgccagagtg
    45961 gcaaacagta gcgcgttatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    46021 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    46081 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    46141 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    46201 atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    46261 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    46321 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    46381 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    46441 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    46501 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    46561 gccctcttcg ataccaggga ccgacaacag gcgtgcatcc attcctggta gaatacgccc
    46621 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcattcggt
    46681 cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    46741 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    46801 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg
    46861 gtcatacacc aagtccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    46921 tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    46981 taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    47041 gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt cagaaccgga
    47101 ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca ggcgcggcat
    47161 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    47221 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    47281 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaataa cactggtcag
    47341 cccttttacc ccggcagtgt agttcatcat tgcgggaatg cggcgacgcg cgatggcccc
    47401 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    47461 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    47521 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    47581 tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac gcgcttccat
    47641 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    47701 cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga acaagcgacg
    47761 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    47821 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    47881 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    47941 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    48001 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    48061 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    48121 cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    48181 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    48241 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    48301 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    48361 gtaccgaata atctcaccaa tcaatacctc tgggatcacc actttagcaa cctgaagcca
    48421 aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc
    48481 tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa acacgccacc
    48541 ccttgaacca acgggcgttt tccgtaacac tgaaacaatg taagcgttta cccactaagg
    48601 tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt
    48661 ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg ctgtgcatag
    48721 tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac
    48781 cactgaaacg gtcggtctga tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt
    48841 gaaagcggtc gaacaggtgg cttatcacac cggtaatttc ttattgattg gcaacggtta
    48901 ccacaacgaa caaaaagagc gtcaggctat tgagcaactg atccgccatc gctgtgctgc
    48961 gttggtcgtc catgccaaaa tgatcccgga tgccgattta gcctcattaa tgaaacaaat
    49021 gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt gtattgctct
    49081 tgacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag gtcatacccg
    49141 cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta
    49201 ttacgatgcc cttgctgaaa gtggtattcc ggccaatgat cggctggtga catttggcga
    49261 accagacgaa aacggcggcg aacaggcaat gaccgagctt ttgggacgag gaagaaattt
    49321 cactgcggta gcctgttata acgattcaat ggcggcgggc gcgatgggcg ttctcaatga
    49381 taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg tgctggtgtc
    49441 acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtaacga tggcgaccca
    49501 ggctgccgaa ctggctttgg cactggcgga taatcgccct ctcccggaaa ttactaatgt
    49561 atttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg aggcaagtca
    49621 tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcgatgg
    49681 tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg
    49741 gacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca
    49801 gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc ccttcctgct
    49861 gggtaaagac atcgcccgat tcacataacg gcccggcgac gacggtttcc accgttggcg
    49921 cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa ctaccgtaca
    49981 ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg cggctaccca
    50041 tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc aggaagcgac
    50101 ccggttcaat ttccagtttc acagggtggc ccaaatggcg agcgatttgc tcacgcgcgg
    50161 cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa
    50221 tagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg atgacctgac
    50281 gcaccatagc accacacacc tgctccagat gggcataatc aacgccagaa ccaatgtgca
    50341 tgtgaatgcc gactagctgc agatgatgac gttgtatcac gtccagtgcg gcgggcagat
    50401 cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgtc
    50461 cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa acctggccca
    50521 gttggtcgag catatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt
    50581 caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg tgcgtttgcg
    50641 gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa tccactttca
    50701 cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc tgtgcaaagc
    50761 gcaccacttc aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga
    50821 cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg gtgagatcgg
    50881 tatcggtgct gaacagtgaa tgtggcataa caaactccag atgagtgctt ttttatgatt
    50941 acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgaatctatg caaaaatgat
    51001 atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa
    51061 tttttcatgc ggtaatgacc gccggaagcc tgactgaggc agcacacctg ctacacacct
    51121 cacagccaac cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt
    51181 ttgagcgcgt acgtggacga ttacatccta ccgtgcaagg actgcgtctg tttgaagaag
    51241 tgcaacgatc ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt
    51301 ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc
    51361 agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc gtgccccagg
    51421 aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaga
    51481 cactccatac gcctgcggga acagaacgta ccgaattact ctctttagat gaagtgtgtg
    51541 tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat gattttcagg
    51601 gtgagaatta catcagcctt tcccgtactg acagctatcg ccagttgctg gatcagctat
    51661 ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct
    51721 gcgcaatggt acgggcgggg gtaggtgttt cggtggttaa cccgctcacc gcactagatt
    51781 atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc accgtcagcc
    51841 tgatccgccc cctgcaccgc ccgtcatcag cgctggttca ggcgtttagt gggcatttac
    51901 aagcggggtt accgaaactg gtcacttctc ttgacgctat tttgtcgtca gctacgacag
    51961 cataaaagcg acagcatcct cggcatggat cgccgcggta tcaaacacag gcaaaacact
    52021 gcgctcttct ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc
    52081 ctgttctgca aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattt
    52141 ccccagacac agttcttcaa aaataatctg attaattttc gcccgttcat ccgcttcagg
    52201 aataagacaa ttgatggaaa attgttccgt cagccgcccg cgataaaaat cctgttccat
    52261 ggtgtaacgc gtacccagca gcgccacacg agtcattccg gccccggtaa ttgcacgtcc
    52321 ggtggcatcc gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg catccgccac
    52381 tttatgcatc gtattggtac ataacacaat accttctgcg cccgcccgct gtaagccaag
    52441 cgccgcctca gccagaatgt ccccggtttt atcccattcc ccgcgacgct ggcactcttc
    52501 tatttcatga aaatcgacgc tatgtagcag catttgcgca gagtgaagcc caccaagccg
    52561 ctgtttaatg ccttcattta tcagacgata gtaaggaatg gtggattccc agctcattcc
    52621 tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc aacaaggctg
    52681 aataaggaac gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag
    52741 ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg
    52801 cctgccatca gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cagaatgagc
    52861 cagaaagtaa tgcccacaaa cgcaatgttc aacgcagtgt agagccagaa cgtaccggca
    52921 gcgccaatgc tatcaagcag tgtcaggaag gccgcgccga taatcatatt cgacacccag
    52981 tttgtcgtcg tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg aatttcagag
    53041 cacaggatcc acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc
    53101 atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg
    53161 caatagccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggt
    53221 ttacgacctg ctttatctac cgtaaagacc gcaatgaagg tcgcgaacat aaaggtcagt
    53281 cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag
    53341 atacgcggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac
    53401 aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg
    53461 ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac
    53521 gtatcgcgca gcatacgcaa cacttcttcc gcctcaatat gacgcccctt ttccgccagc
    53581 cagcgcgggc tatttggcag gaagaccacc agaataatca gcagaactgc tggtaaagca
    53641 agaaccccaa cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg
    53701 ccagcacgat gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt
    53761 acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga cagcaatgcc
    53821 cagcaccaca cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg accctataga
    53881 accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca ggcggaacga
    53941 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca
    54001 ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat
    54061 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    54121 attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    54181 aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg aacaattcac
    54241 acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca tgccgttatg
    54301 tgatggatat tccaatttta aaattaagtt gaattattga gattattatt aaccacctaa
    54361 ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    54421 aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg cggcagggtc
    54481 ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc aatggtataa
    54541 ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat caggtcactc
    54601 ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg ttgttcatct
    54661 gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    54721 atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac tccgcttttt
    54781 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    54841 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
    54901 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    54961 gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca
    55021 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    55081 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
    55141 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
    55201 ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc acttaaaatc
    55261 ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
    55321 ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    55381 ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    55441 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    55501 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    55561 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt tccagtacat
    55621 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    55681 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
    55741 aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
    55801 cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacag
    55861 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
    55921 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
    55981 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    56041 ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt tttgcgtgcg
    56101 caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    56161 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
    56221 ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga ttaaaaaaaa
    56281 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
    56341 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
    56401 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta gcccccgccg
    56461 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
    56521 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
    56581 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
    56641 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
    56701 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
    56761 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
    56821 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
    56881 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
    56941 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
    57001 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
    57061 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
    57121 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
    57181 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
    57241 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
    57301 gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt gattgcagaa
    57361 acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
    57421 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
    57481 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
    57541 ccacgaaagc agccgatagg tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca
    57601 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt
    57661 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
    57721 tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg gcgttaatct
    57781 cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
    57841 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta
    57901 cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt cggcaggggc
    57961 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc tacataaagc
    58021 cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa cgggcgcggt
    58081 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
    58141 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
    58201 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat
    58261 tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg gatttttggt
    58321 attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta gttggcaacc
    58381 tgatctatta acaacccagg tagagttcaa tcagaatacc cttcaccaga tatggatatc
    58441 gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt ttgccataga
    58501 cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta tgaaagtcgc
    58561 ttatctcatc atctgcataa gtgtactgtt ctttgtcttt agctgcctgc tttctattcc
    58621 atcttcgtat attgaagcgg ctaaagaaga aggggtcacc attttatcgg cgctttctat
    58681 gctgccgaac gccccagcat ggttgtcaat ttccgggatt attgtcgcag tagttgcgat
    58741 gtcgaaatca ttcctgggta cgtactttgg cgttattgaa ggtgccacag aggtcgtcaa
    58801 aacaacacta cagcaggttg gtgtaaagaa aagtcgtgca tttaaccacg cactatcaat
    58861 tatgttggta tcgctgatta ccttcattgt ttgttgcatt aacccgaacg cgatttcgat
    58921 gatttacgcg atcagcggcc cgctcattgc catgatactt ttcatcatgc ctacgctgtc
    58981 aacgtatctc atcccggcgc ttaaaccctg gcgttccatc ggaaatctga ttacgctgat
    59041 cgtgggtatc ctgtgcgtat cggtaatgtt ctttagctaa tatccgttgt ggatggttca
    59101 aaattgctac aattttgaac cattccattt ttcataatat tcatttgtta ttattccgcc
    59161 ctatcacttc tctcgtcata tcctagaatt atcagttagt taaacacaca aagagatctg
    59221 aacatcttaa ataagactat ttaagatgca taacttagat tcgcaagata tcttctggtc
    59281 attcaaaaac aatttgcgat atagatattc tcataccatg agatagttat ctggaccacg
    59341 ttcgaccatg caagattaaa tcagtgaaac ataatcatat ttaataacga gaaatgcatt
    59401 tttaaatatc ttaatcagaa ttatagcaaa tacagattaa tcgca
//
LOCUS       DADLXX010000012        58004 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLXX010000012 DADLXX010000000
VERSION     DADLXX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.012"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(24..278)
                     /locus_tag="JNX27_000701"
     CDS             complement(24..278)
                     /locus_tag="JNX27_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7882014.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JNX27_000702"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JNX27_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7882015.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JNX27_000703"
     CDS             1228..2694
                     /locus_tag="JNX27_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7882016.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /locus_tag="JNX27_000704"
     CDS             2812..3798
                     /locus_tag="JNX27_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7882017.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JNX27_000705"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JNX27_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7882018.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JNX27_000706"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JNX27_000706"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7882019.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JNX27_000707"
     CDS             5709..7265
                     /locus_tag="JNX27_000707"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7882020.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JNX27_000708"
     CDS             7347..9161
                     /locus_tag="JNX27_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7882021.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JNX27_000709"
     CDS             9162..10256
                     /locus_tag="JNX27_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7882022.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JNX27_000710"
     CDS             10256..11281
                     /locus_tag="JNX27_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7882023.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JNX27_000711"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JNX27_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7882024.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JNX27_000712"
     CDS             complement(12876..13220)
                     /locus_tag="JNX27_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882025.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNX27_000713"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNX27_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY7882026.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNX27_000714"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNX27_000714"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7882027.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JNX27_000715"
     CDS             15616..17376
                     /locus_tag="JNX27_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7882028.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKDGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JNX27_000716"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JNX27_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7882029.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEINVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNX27_000717"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNX27_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7882030.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JNX27_000718"
     CDS             19113..19340
                     /locus_tag="JNX27_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7882031.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JNX27_000719"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JNX27_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7882032.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JNX27_000720"
     tRNA            21195..21271
                     /locus_tag="JNX27_000720"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNX27_000721"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNX27_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7882033.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVDKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JNX27_000722"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JNX27_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7882034.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JNX27_000723"
     CDS             complement(25143..26195)
                     /locus_tag="JNX27_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7882035.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDTNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNX27_000724"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNX27_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7882036.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JNX27_000725"
     CDS             complement(26746..28689)
                     /locus_tag="JNX27_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7882037.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNX27_000726"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNX27_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7882038.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNX27_000727"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNX27_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7882039.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNX27_000728"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNX27_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7882040.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNX27_000729"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNX27_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7882041.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNX27_000730"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNX27_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7882042.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNX27_000731"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNX27_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7882043.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNX27_000732"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNX27_000732"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7882044.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNX27_000733"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNX27_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7882045.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNX27_000734"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNX27_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7882046.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNX27_000735"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNX27_000735"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7882047.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNX27_000736"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNX27_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7882048.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNX27_000737"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNX27_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7882049.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="JNX27_000738"
     CDS             37391..37555
                     /locus_tag="JNX27_000738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882050.1"
                     /translation="MHTPIGVKPAAGSKEWREAWQKRAFAHISNVYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="JNX27_000739"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="JNX27_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7882051.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39359)
                     /locus_tag="JNX27_000740"
     CDS             complement(38328..39359)
                     /locus_tag="JNX27_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HAY7882052.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFELVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39593..39952)
                     /locus_tag="JNX27_000741"
                     /pseudo
     CDS             complement(39593..39952)
                     /locus_tag="JNX27_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNX27_000742"
     CDS             complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNX27_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7882053.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPHAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNX27_000743"
                     /pseudo
     CDS             complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNX27_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNX27_000744"
     CDS             complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNX27_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7882054.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNX27_000745"
     CDS             complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNX27_000745"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7882055.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNX27_000746"
     CDS             complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNX27_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7882056.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46647..49319
                     /gene="rcsD"
                     /locus_tag="JNX27_000747"
     CDS             46647..49319
                     /gene="rcsD"
                     /locus_tag="JNX27_000747"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7882057.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49336..49986
                     /gene="rcsB"
                     /locus_tag="JNX27_000748"
     CDS             49336..49986
                     /gene="rcsB"
                     /locus_tag="JNX27_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7882058.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNX27_000749"
     CDS             complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNX27_000749"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7882059.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53310..54086)
                     /locus_tag="JNX27_000750"
     CDS             complement(53310..54086)
                     /locus_tag="JNX27_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY7882060.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54091..55740)
                     /locus_tag="JNX27_000751"
     CDS             complement(54091..55740)
                     /locus_tag="JNX27_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY7882061.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLAFLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55741..58004)
                     /locus_tag="JNX27_000752"
                     /pseudo
     CDS             complement(55741..58004)
                     /locus_tag="JNX27_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14167 a        15655 c        14267 g        13915 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccatc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gctcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcaatg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gccaggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact tggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggca tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctag gtatcgatac ctggtggtat gacgttaata
     9121 aagcggcaaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcg tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaaca
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    13861 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccctttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa agacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgaccccccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgct ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    17701 taaagaaatc aacgttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagaca ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ttggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttatcc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga acaagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata atgaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcaacaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 gtatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaggtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggt
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtacctctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc aaggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac ggttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cgggcggata ccgttactgg ctttgcgcca gcgaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgca gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat gtttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgca gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcaactgcg gtttcggcgt actatctgct
    38401 gcttgcggtt tgtcgtattt cagccccagc acttcgctgg tgtactgcaa ctcgcgttct
    38461 gttgccgcta catcaccgtt cagcttgcgt ccataagacg gaacgatcgc tttcaacgta
    38521 gcctgccatt gcgggctgga aacgcgatcg ccaaatactt tttccagcag attcaacata
    38581 atcggcgcgg cggttgacgc ccctggcgat gcccccagca gcgccgcaat ggtcccttgc
    38641 tggtcactga ccacttcggt acccagacgc agtacgccac ctttctcggc atcacgcttg
    38701 ataatctgca cacgctgccc cgcttgccac aaacgccagt cctctttttt cgcttgcgga
    38761 tagtactctt tcaacgcttc aaaacgatcc tcttcactca acatcacctg actcaccaga
    38821 tatttcacca gctcgaaatt atccagcccg acgtgcatca tcggcatcac gttagaggtg
    38881 gtggtggaac tcatcagatc ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg
    38941 gcaaatggcc caaacagcac tacgcgttta ccgtccagaa cacgggtatc gatatgcgga
    39001 accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa ctttcgccag atggcgatta
    39061 accacgtctg gattttccga aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct
    39121 ttcgcttccg gaatccccga ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata
    39181 aagacaaatt tggtgcggat gttctgtgca gtgccgtttt tcagatcggc aacggtaacg
    39241 gtccaggtgt tatcgtcatt acgctttagg gcgcggactt cgctgctgag ttgcagcgag
    39301 aagttagatt tcttctgtaa ggaagcaatt aactggcggg tgatctcacc atagttcaca
    39361 tcagtgccaa tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
    39421 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
    39481 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
    39541 cgatattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
    39601 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
    39661 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
    39721 tgttccagcc gttcgaactc tcctgcgcgc ccccctccag gcgctccacc atggtcatcg
    39781 accattcagg ctccagctcg cgtaaatagg tccccaacgt ggcgctcata atgccgccgc
    39841 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
    39901 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
    39961 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
    40021 aagtaacaac atttaaataa tgtttataaa ttatattcta attttagaaa cattataatt
    40081 tcgttgatta attatagggt tattagaaat gaagggattt ttagcatatt gacctgacgg
    40141 cagcagtccg ccaggtcagc gtcgtaatat tatgccgtcc gggcaacggc atcacgcgaa
    40201 gcggcatcgc gctcttcgcc cgtcagctcg ctaagttgcc cattgcgcat ttccagcagg
    40261 cggtcggcgt ggataaagta atgatcatca tgactgatag cgaaaatagt tttacccatc
    40321 tcctgcatca gcggcagcaa cacctgataa aactcacgac ggaagtgtgg atcctgatcc
    40381 gccgcccatt catccagcag gataatatcg cgttcttctg ccagcgccag caacagcggc
    40441 acgcgttttt tctgcccttt tgataacttc aggttaacaa tacgcccgtt gcttaactca
    40501 agcttatgag ccattttcag ctgcgccagc cacttctcaa ccagttgcgg gttagcgggt
    40561 tgcccctccg gccccagcag ttgatcaaac agccagacat cggtaaacac tgccgaaaac
    40621 agtttgcgat aatcttccgg ttgttcgccg ctgacaggtt tgccatccag caagatttcg
    40681 ccgctttgtg gctgatacaa gcccgtcaac aacatcgcca gcgtcgattt tccgctaccg
    40741 ttgccgccaa tcagaaacag cagctcgcca cgtttgatgg tgagattaat cggaccaacg
    40801 gaaaacgcgt tatcctgata agcaaacgtc acgttacgca gctccagcgt ttgccagttg
    40861 ggaaacgcat gcgggcgcgg aaactcagct ttgaaaggcg cgagcgcgaa tttgttcagc
    40921 ttgttaaacg ccacctgcgc cgtcagcagc gtcggcaatg cgccaaccgc cgaaagcagc
    40981 ggcgtacgca ggaataacag cgtcaacgaa taggtcgcag caacgttggt atcagcccaa
    41041 ccgagactgt tcgccatcca gaacaccagg ccgatagcgc ccagcatcat gatgtttgac
    41101 cagttcacgg cactaagatg gaaggtgtct gcgcgaataa tatgatggcg atactcttgc
    41161 gcatcgggga tgtagaggtt gttaaacaca tactcggcgc gttcccggtt cagagtcagc
    41221 tctttgcgcc cttcaagtac agtttgaaaa tccgtgtaca gcttgtcttc ggtttcacgt
    41281 agggtcgcca tatgtttgta cacccgcgcc accagcacaa aaccgcccca gatggtgatc
    41341 gccatccaga tagccgttac cagcaacatt ttgcccgaca gcatccacag atacgccgat
    41401 gaaccgatag tgagaatgat cccctgcccc agttccggca gacgcacaaa agcaatggtg
    41461 atattgcgca catcgctggt taatcccgcc agcaacgagg cgctaccgag ttgttcaatg
    41521 cgctcgacgt gggtatccag aatccgcttg ataaattcac tacgcagtcg gtaaacgaag
    41581 tgatgcccca aagtggtgag cgccagttgc gatccgagag tgactgccat caacagcagc
    41641 aataatccca gaaactccgg taacaccagc agactggtat ccgccgtttc gataaggcgc
    41701 tgattgataa aagcaattaa gccaatgcct aatgccgcac tggcgaggct tagcgccatc
    41761 acactgataa atggccagcg atactgccgc cagacaagta caagaagttc catgcagaaa
    41821 acccggacaa tgaattacag cccgcagttt aaacatcttc gcgcgcacag caataataat
    41881 tcttattttt attctttttt acctgcctga cggaatgtca ggttgtagcg gcagtcggtg
    41941 gtgagtggat gaaaccccgc tttcaacggt tgaataccgt gataaaacag ccgcgattca
    42001 ccgccccagg ccaccaaatt gaaaaatcgc gggtaagccc agagaaacag aaacaattgg
    42061 cgcgcgcaga tccggttcgt ctttatcctg atgcagcgac agtttcgcgc caggagcgta
    42121 gcggttgata agacaagcat ctggctggaa atctggatag cccgccgccg tagccgcacg
    42181 ttgacataaa tcatgaaagc tttgtggcat ggcgggccac ggtttatttg tttgcggatc
    42241 aatgggcgaa tagagataac cttgccgatg ggtcgtccag cccagatgcc cacagttggt
    42301 catcgccacc gacatggtat atccgcctgg agcgaccatc tggcgaaacg gcgactggct
    42361 ggcaacgtca ttaatatcgc ggattagttg ctccgcagca ttaaaagcaa aacgccgtaa
    42421 aattaccgca ccagccgcca gtggctcttg ccacggttca gcatcggcaa acagatccaa
    42481 cattacctct cctcattttc agcttcgcgg cgcagcagtt gcgctttacg cgacacgccc
    42541 cagcggtaac cggaaagtgt gccatcacca cggaccactc gatgacaggg tattacgata
    42601 gccagcttgt tggcggcaca ggcgctggca acggcccgta ccgctttcgg tttgccgatg
    42661 gcgttagcca gttgctgata actgacggtt tcaccacaag gtatcgtgcg cagtgcctgc
    42721 cagacttgtt gctgaaaagc agtgccgcga atatccagcg gtaacgtcag cggcgtatcg
    42781 cgttgattga ggctggcgat cacttcacgc acatgttgct gaaacgtcag atcggcaggc
    42841 gcgttgtcgg cagcgggaaa catctgctgc aactcgctga ttaatgtcgc gtcatcatcg
    42901 cccagcaata tcgcgcaaat cccccgctcg ctttctgcca ccaggcaacg acccagctca
    42961 caatcagcca gcgcgtaacg caccgccaga ttttcgccac cgtgacggaa ttgtttagcc
    43021 gtcatgccca gcgtttcatc ggctttgcgg tagtaactgc tgctgtcggg gaatccggcg
    43081 ttaagaatag acgtcgtcac gctctcccct ttcgccagcg attcgcgcaa acggcgagcg
    43141 cgccaggcct gttgccaggc tttaggcgtc attccggtag tagctttaaa caaccgatgt
    43201 agatgaaatg gactcatcgc cacctggtcg gctaaggctt ccagcgttac aggcgtttcc
    43261 tgttccagca gtcgacacgc gtgggtgatt ttatccaacc gatgttgccg gggattggct
    43321 ttgtctggct gacaacgttt gcaggggcga aatccggcgg cgagtgcctc gctggcattt
    43381 gcgtagaagg agacattttc ccgcaaggca tgtctggcgc ggcaagacgg acggcaaaag
    43441 atacccgtgg tgcgcacggc gaaaacgaat tcgccgtcgg cattcgggtc gcgggctaag
    43501 acagattgcc agcgttgatc gtcagttaag catgtggcgt ttttcataat cagctccctg
    43561 gttaaggata gcctttagtc tgcccggtca ccatcacgca aaaaccaaca atcttgcgct
    43621 ttaatttttt tcgctgacaa ggaagctttt aaactgtggt gacatccagg ttttaaagct
    43681 atcgccttct ttggtgatca tataaaccgc cagcccttcc cggcgaacaa cctctttggc
    43741 tttctccggc ccgagtacca tcaagccagt atcccaggca tcggcttcca gcgccgtcgg
    43801 agcaatcacc gtcacggata ccagattgtg ttcgatggga cgcccggttt gcggatcgat
    43861 aacatgggaa agacgtttgc cgtccagttc gtaatagtta cggtagctgc cagaggtgct
    43921 aatcccatga ccgttgatat ccaccacagc ctgaaccgcg ttttctttat cggttggttt
    43981 ttgaatcgct acccgccacg gctggccttc accgttcata ccacggctgt tcagcgcgcc
    44041 gcccaccgac accagatagc gggaaatccc ttcctgctcc atcaagcgtg ccaggtgatc
    44101 cgccgcataa ccttcaccga cggtggagag atcgacatat aaatccggca ggtctttttg
    44161 cagatattgc tgatgcgact gattgatgac cgtcaggtgc tgtaagccgg ttttggcttt
    44221 catcgcatcg atctgttcct ggctcggaat ttgaaccggc tgttgttccg ggccaaagcc
    44281 ccacagattc accagcggcc cgacggtaat atccatcgca ccatcggtcc ttgccccaat
    44341 gcgcagcgag gtggtgacga tatcggccat cgcttcactt accggccacg gcgacaaact
    44401 ttgcgagtcg ttaaagcgca tcagcgcgga atctttttta taggtcgaaa gcagctgatc
    44461 gtcggcgtcc agctgggtct gaatcttttc tttaagttcg gcactgcgtt tggcatcaat
    44521 gcccgggatg ctggcacgcc agaaggtacc catggttttg ccttcaagaa cggtaacttc
    44581 agtagcgtgg gttttggcgg gttgtggttt ttgatcgcaa ccaacaaaga agagcgcggc
    44641 aaccagcagt gccacgcggg taaagcttat ttccatacgt gattatcctc atgcgaacgg
    44701 tcgcaagagt acaccaaaaa actgtgtttg tacgctgaaa acaatgaaaa aagggcccgc
    44761 aggccctttg ttcgatatcc atcgagatta gaactggtaa accagaccca gagctacgat
    44821 gttatcagtg ttgatgccag cgtcacgagt gaactggttg tcgtccagca ggttgatttt
    44881 gtagtcaacg taggtggaca tgtttttgtt gaagtagtag gtcgcaccaa catcaacata
    44941 tttcaggata tcttcgtcgt cgtagttacg accattgatg acacccaggt ttttaccttt
    45001 agactgcagg taagctaggg acggacgcag accgaagtcg aactggtact gagcaacagc
    45061 ttcgaagttc tgtgctttgt tcgcccaacc caagttgcct acgcgagttg cgttgtaggt
    45121 ctgggtgtac tgagcagcta ggtagatgtt gttcgcgtcg tatttcagac caccggtgta
    45181 ggtttcagcg cggtcgccat taccgatgta acgctcatca taacggttca gaccgaagtt
    45241 ctggtcatca gtacgtttgg agctggaaac tgcagcaccg ataccgaagc cttcgtaatc
    45301 ataagtaata gaaccgccaa cgccgtcacc gttctgacgc agagcttcac gaccgttgtt
    45361 ggtcatgcct tcaccttccg ggctaccgtt tttgccctga tactgaacag cgaagttcag
    45421 accgtcaacc agaccaaaga agtcggtgct acggtaggtt gcgaagccgt taccacgctg
    45481 ctgcatgaag ttgtcagaac cgtaagtgtc accaccgaat tctggcagta cgtcggtcca
    45541 ggaagttacg tcgtaaacta cgccgtagtt acgaccgtag tcgaaagaac ctacatcctg
    45601 gaatttcaga cctgcgaatg ccacacgggt ccaggagttg ttttcgtttt cagcgctgtt
    45661 gccctggatc tgatattccc actggccgta accggtcagc tggtcagtaa cctgagtttc
    45721 acctttgaag ccaagacgca tgtaggtctg gtcgccatct acagacttgt cgtcagagaa
    45781 atagtgcagg ccgtctactt taccgtacag atctaatttg ttgccgtctt tgttgtaaac
    45841 ttcagcagcg tttgctgctc ctgctaccag cagagctggg accaggaggg acagtacttt
    45901 aactttcatg ttattaaccc tctgttatat gcctttattt gcttttttat gccactgcat
    45961 actgattaac cctcattaat cagtcggcaa gtccattctc cccaaaaatg cagaataatc
    46021 caacacgaat atgatactaa aacttttaag atgtttcatt tatcgctata gatgtttcaa
    46081 aatgtaaata caagggaact ttttaagatt attgcggaat ggcgaaataa gcacctaaca
    46141 tcaagcaata ataattcaag gttaaaatca ataacttatt cttaagtatt tgacagcact
    46201 gaatgtcaaa acaaaacctt cactcgcaac tagaataact cccgctatca tcattaactt
    46261 tatttattac cgtcattcat ttctgaatgt ctgtttaccc ctatttcaac cggatgcctc
    46321 gcattcggtt tttttaccct tctttacaca cttttcatta ttctgtgcta ccacagaaaa
    46381 actataacgc ttgttaacta tttcacaaat aattaacatc agcataattt ccagcaatct
    46441 ttgtttattt acaattaatt tcgttgggct ttttgtaggt tatttgtaca gcaaaatggc
    46501 gcttgtacat ctatttcccc ccatacagga tgataaatat cacgggagaa tagagaatca
    46561 tcaatcaggt aagagtctgg aatttcacac tgtacccttt atactgccct atcacttcgc
    46621 gaagttttaa caggtcataa acacgaatgc gtcagaaaga gacaacggcc acgacccgct
    46681 tttcactcct accggggagc attacccgct tctttttact gttgatcatt gtgttactgg
    46741 tgacgatggg ggtaatggta caaagtgccg ttaacgcctg gctgaaagat aaaagttacc
    46801 agattgtcga cattacccac gcaatccaaa agcgcgtcga tacctggcgt tacgtgacca
    46861 ggcagatcta cgacaacatt gccgcgacga cctccccctc ctccggcgaa ggtttacaag
    46921 agacgcgcct gaaacaggat gtctactatc tggagaaacc acgccgcaaa acggaagcgt
    46981 taatctttgg ctctcacgac aactcaacgc ttgagatgac tcagcggatg tccacttatc
    47041 tggatacatt gtggggtgca gaaaatgtac cgtggtcgat gtattacctg aatggtcagg
    47101 ataacagtct ggtgctgatc tcaaccctac cccttaaaga tctcacctcc ggatttaaag
    47161 aatcgaccgt cagtgacatt gttgattcac gtcgtgcaga aatgttgcaa caggccaacg
    47221 ccctcgatga acgcgaaagt ttttctaaca tgcgccgcct ggcctggcag aacggtcatt
    47281 actttacctt gcgtaccacg ttcaaccagc cgggacatct ggcaacggtc gtggcttttg
    47341 atctgccgat taatgatttg atcccaccgg gtatgccgct ggacagtttc cgccttgagc
    47401 cagacgcgac ggcaacggga aacaatgata atgagaaaga agggacggat agcgtcagta
    47461 tccactttaa cagtacgaag attgaaatct cctcggcact caactctacc gatatgcgcc
    47521 tggtctggca ggttccttat ggcaccttat tgctggatac gttgcaaaac attctgctgc
    47581 cactgctgct gaacatcggt ttgctggcgc tggcgttatt tggctatacc acattccgcc
    47641 atttctccag ccgcagtaca gaaagcgtcc ccagcacggc ggtcaataac gaattgcgca
    47701 ttttacgggc aattaatgaa gagatagtct cactgttgcc gctcggcctg ctggttcacg
    47761 atcaggaatc gaaccgcact gtcataagta acaaaattgc cgatcatttg ctgccgcatc
    47821 tgaatctgca aaacatcacc accatggcgg aacagcatca ggggattatt caggcgacga
    47881 tcaataacga gctgtatgag atccgcatgt tccgcagcca ggtcgcgccg cgcacacaaa
    47941 ttttcattat tcgcgatcag gatcgcgaag tgctggtaaa caagaaactc aagcaggcgc
    48001 agcgtctgta tgagaaaaac cagcaggggc ggatgacctt tatgaaaaac attggcgatg
    48061 cgctgaaaga acccgcacag tccctggcgg agagtgcggc taaactcaac gccccggaaa
    48121 gcaaacaact ggcgaatcag gcagatgtgc tggtgcggct ggtcgaagaa atacagttag
    48181 cgaacatgct tgcggatgat agctggaaaa gtgagacggt gctgttctcc gtgcaggatt
    48241 taattgatga agttgtgcct tcagtgttgc ctgccatcaa gcgtaaaggt ctgcaactgc
    48301 tgattaacaa tcatctgaaa gcacacgata tgcgccgcgg cgatcgcgat gccttacgac
    48361 gtattttgct gctactgatg caatatgccg tgacctcaac gcaattggga aaaatcaccc
    48421 ttgaggttga tcaggatgag tcctctgaag accgcctgac attccgcatt ctggacacgg
    48481 gagaaggcgt aagtattcat gaaatggata atttgcactt cccgtttatc aaccagaccc
    48541 aaaacgatcg ttatggaaag gcggacccgc tggcattctg gctgagcgat caactggcac
    48601 gtaaactggg cggtcattta aacatcaaaa cgcgggatgg gcttggtaca cgctactctg
    48661 tgcatatcaa aatgctcgcc gctgacccgg aagttgaaga ggaagaagag cgtttactgg
    48721 atgatgtctg cgtaatggtg gatgttactt cggcagaaat tcggaatatt gtcactcgcc
    48781 tgttagaaaa ttggggtgca acctgtatca cacccgatga aagattaatt agtcaagatt
    48841 atgatatctt tttaacggat aatccgtcta atcttactgc ctctggcttg cttttaagcg
    48901 atgatgagtc tggcgtacga gaaattgggc ctggtcaatt gtgcgtcaac ttcaatatga
    48961 gcaacgctat gcaggaagcg gtcttacaat taattgaagt gcaactggcg caggaagagg
    49021 tgacagaatc gcctctgggc ggagatgaaa atgcgcaact ccatgccagc ggctattatg
    49081 cgctctttgt agacacagta ccggatgatg ttaagaggct gtatactgaa gcagcaacca
    49141 gtgactttgc tgcgttagcc caaacggctc atcgtcttaa aggcgtattt gccatgctaa
    49201 atctggtacc cggcaagcag ttatgtgaaa cgctggaaca tctgattcgt gagaaggatg
    49261 ttccaagaat agaaaaatac atcagcgaca ttgacagtta tgtcaagagc ttgctgtagc
    49321 aaggtagcct attacatgaa caatatgaac gtaattattg ccgatgacca tccgatagtc
    49381 ttgttcggta ttcgcaaatc acttgagcaa attgagtggg tgaatgttgt cggcgaattt
    49441 gaagactcta cagcactgat caacaacctg ccgaaactgg atgcgcatgt gttgattacc
    49501 gatctctcca tgcctggcga taagtacggc gatggcatta ccttaatcaa gtacatcaag
    49561 cgccatttcc caagcctgtc gatcattgtt ctgactatga acaacaaccc ggcgattctt
    49621 agtgcggtat tggatctgga tatcgaaggg atcgtgctga aacaaggtgc accgaccgat
    49681 ctgccgaaag ctctcgccgc gctgcagaaa gggaagaaat ttaccccgga aagcgtttct
    49741 cgcctgttgg aaaaaatcag tgctggtggt tacggtgaca agcgtctctc gccaaaagag
    49801 agtgaagttc tgcgcctgtt tgcggaaggc ctcctagtga ccgagatcgc taaaaagctg
    49861 aaccgcagta ttaaaaccat cagtagccag aagaaatctg cgatgatgaa gctgggcgtc
    49921 gagaacgata tcgccctgct gaattatctc tcttctgtga ccttaagtcc ggcagataaa
    49981 gactaatcat ctgtaggcca gataagacgc gttagtgtct tatctggcat ttgcaccgat
    50041 tgccggatgc ggcgtaaacg ccttatccgg cctacgattc ccattatttc aacagattac
    50101 attaaagtag gccagataag acgcgtcagt gtcttatctg gcatttgcac tgaatgccgg
    50161 atgcggcgta aacgccttat ccgtcctacg aatcccgcga tttcctgacc ctctcggcat
    50221 ataccgtcag cgtctgtttt atcacatcca gcgttaccgg cttcgagagg cagctgtcca
    50281 taccggactc cagacaccgc tgcttctctt cagccaacgc attagcagtt actccgatta
    50341 ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg
    50401 gcatgttgac gtcgctaagc acgatatcaa tatgattctt actaagtaca ttaagcgcat
    50461 cgacgccatc attcgcggtt ttacattgat agcccaacga tcccaactga tctgccagca
    50521 gacgtcggtt aatcggatga tcatccacga ccagaatcat catatcgtcg ttatcgctga
    50581 ccgctttgtc cgtcgacggc agagcgttag caggatcgtc gctctccatc tcgatcaaat
    50641 aaatacgcgc caacaatgcc ggtagctcat gcggagcagc cacactgtgt acccactccc
    50701 ctggcgcttt ctccagcgga ataccaatat ggcgacgaca gaaggtcact accgctctgc
    50761 cctgccattt tttactcact acctcgtcag tgattaacac atcttcggga gtcggttcct
    50821 gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc aggaactgac
    50881 agagcgacgc attgcggacc gccagccagc agcgtttacc actcaaccct tccacgcctt
    50941 ttttctgcgg gtactgagcg ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc
    51001 ccggttctga atctaccgag atatcgccgt ccatcatgct gatcagtttt tcacaaatcg
    51061 ccagacccag accggtcccc tggaagtttc gctgtacgcc cgttccaacc tggaagaagg
    51121 gatcaaacaa gcgcaccact tctttcgccg gaatccccac gccggtatcg cgaacacgga
    51181 tagagagata atcgccatca gcgcgaacat gcaaaactat acagccggta tcggtgaatt
    51241 ttatggcgtt actcaacagg ttggagatga cctgctgtaa acgcatcggg tcgccattta
    51301 aggccactgg cacatccggt tcaataaagc agtacaagcc taactgcttg cgtaccacca
    51361 gcggtaaata gttggcggtg atgtggttca tcacttcacg cggtgaaaac tcacgcggtt
    51421 cgatcttcaa ctgttccgat tcaatcttcg agaaatcgag aatatcgctg ataattttca
    51481 acaacaggct ggaagagttg ttcattgccg tcaccagacg atcgacgcct ttcggtaact
    51541 ctttggtttg caacagatcc aggttaccga taatgccata cagcggcgtt cgcagctcat
    51601 gactgacggt ggcaaggaac atcgattttg actggctcgc ctgttccgct gcttgtgcca
    51661 tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac atccaccagc acacaaatgg
    51721 ccacgttttc attacgatag cgcgaatgga cgaagctgat ttgcagattg gtattgttgc
    51781 tggtcaggac atcaacaaaa ttgacctgct gcccacagat aatttgcgtc agtcgttggc
    51841 ggtcctcatg cgtaagcata ttgagatagg tatgcgccag ttcgttactt aaaatattga
    51901 cgccatcagc ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca atcttgcgat
    51961 tgaactgctc atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg aaaatacgtc
    52021 gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc aagcacattc agcaaaattg
    52081 cgttaaggat caacatgcga atgcgttcca gcaccttatc aaccggcacc gaatacacga
    52141 tgcttagcga tgagggtggc agatttttct tcagcaccag ctcccggaac ccttccgtat
    52201 agccaaacca ggagcgttcc tgcatccagc gaggatcgcc cttaatttta ctttctggtc
    52261 cggtaagcga aatcagggta tgaccatttt catcaagaat ggtaaccccc atcggcaacg
    52321 tacccggtaa gaaaaagttc tccatccgga tggtctgctc gacacccaaa agcgcctgca
    52381 accggttcgc cagataaact ggcgtcaacg cgtaaaaata cccgacgcca gggcgcggac
    52441 cttcgctgat ccagtagagg ttactgccgc tatcatcttg tggtgcattt cgatatttat
    52501 tgatgcgttc atgcaacgct ttcaacgcgg tatcgcgttc cactggcata tcacgcagac
    52561 cgaaattggc catgcagagg ttatcactgc cgattaaaaa tacccggttg agatcgtaag
    52621 ccgcagaaaa attatcgcgc cagtagcgca taaaccacgc caatgactcc agagaacctc
    52681 gccaggtgtt actcattgcg gaacaatcgg agtcggcaaa cagcggttca aacgcaggca
    52741 catccgcctg cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc gataagcgat
    52801 tttcggcgat gtacttaagc tctttcatca catcagaggt gcgttgaata aagcgctgag
    52861 cctgatcgga actcagatta aattcctgac gaatttccga ttctcgctga tgtaacgcat
    52921 taacgatgta aaaaacggat gaaaaagcaa tcaacagcca gagcactaac gccaatgctc
    52981 tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg
    53041 cgaagctccg cctcaggtga ccgatggagt gtggttaagg tagcggtaaa agcgtgttac
    53101 cgcaatgttc tgtcaccatt gcaataatgc gtatcgctta gaaaccattg ccttaaccag
    53161 gaaagccgga gtagatggac agttctcagt acgaaaaata agaaatgacc aatctgccga
    53221 cgcttgttaa tggttatatc ctggcgtcgg cagatcgccc agcagtctta tctgaaaaaa
    53281 gagagagcca ggttgtgtga agggatggat caccagtcaa aacttttcac cagcgtcagc
    53341 tcgccagcat tacgcatcgg tacaataaat gtttcctgtt tctcattgac cgatccttca
    53401 tcggtgatca gcgtcagttg ggcggtcgtc aattccgttt cgctgcgccc accatagtag
    53461 ttgatataca cctgatagcg cccgtgaact ggtgctggca tggcgaaaat ctcgggtccg
    53521 taccccgtcg tgacatccat atccagtgca ccactgtttt tcagcacggt gttaccgtac
    53581 caggcgtgtt cgccatcggg cgtgacaaca tggagatcga gatcggtatt gtccgtatcc
    53641 cacgagagaa ccagccgtaa acgtgcacga atagttcctg tacccggcgt tgagtaaaac
    53701 tgcatttttt gccggctttg cccatccggg ctgatgacct gcacgctgtt gctgccttca
    53761 gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa ttctctgcgg catactggca
    53821 ccgttgacca ccagccggcc ctgctgaact ttacccgccg cagcataatt ctttattttg
    53881 ccgctgatct gcgctgagat attttgatca tctgccatgt tgacggacga cgccgggtaa
    53941 ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg gatgccagcc agagagcggt
    54001 gcgtcaatct cgacttcctg cacaccttca ctgtgagcga caggcgaaag tgccaccagt
    54061 aacaacggaa gaaaaatctt tcgcatagca ttagtccatt aataattgtc gggtcagcgt
    54121 ttcgatatag ttctcatcaa gcccggtggg ataaccatca aaggccaagt gcagatactc
    54181 atgggttaaa tccagccgat cctgaagcgt aaagaagtta cgaataaaca gccgtttctg
    54241 ctgccgatcg gtatagggga aacctgagac taaacgacag accgcaaaca cgtctggttc
    54301 gttgtacccc gtctcgcctt gtaatatacg ccgccactgc ggcatttttt tcgccagcca
    54361 ggctttcgct ttgggtaata actggcaggt tgagcgtgga gctccccagc ggctaaggct
    54421 gttgtcggga taagcaaacg cgagtatctg gtcgtaacgc tccccctgcc ccgcctgggc
    54481 cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga ctgccatgat aatgaaccgg
    54541 atcacctgca tagatgagct cctgcgtcca ggcggtcatc gttcgcgccc ccgtggtcgc
    54601 tggcgaagca gaaacacgct gcgtggcgct gctgccgggg atggtcaggc aatcaccttc
    54661 ccgattggcg ttttgctgta aaaaagtacg tatcgccaca gtcatggctt tcgctgcctc
    54721 aggtggcgta cttttcgcct cgcgatccag aactctggca acgtactctt cacgatcgag
    54781 atgcgactgc aatttcagtt tgcccttttc gctcagtaag gtcgtctcac cgtggctgac
    54841 aaacgtaatg tggttaccat tagtaaacgt cacacgatag cgcccattaa gtacgctggg
    54901 ttttactgcc gtcgtactct tttccgctgt gattttcttc agcggatagc gggcaaacag
    54961 ctccacctct acacattgcc cactggcaac ctgcgtcggc actggcaaaa cccgattaag
    55021 caccgtcgca taacgggtta gtaccgtttt gctggtgcca ctgccagtaa cccacagcgg
    55081 tgtaccatcg gttaaccagc cagcaaaacc accctgacgg atttcctgtt tatcatcggc
    55141 aaaccagctc caggttttca cccgtacccg actaccgagc atggatgaga caccgatttt
    55201 cgcctcgtcc agcaccacat ccagtaagac ttcctgagct ttgttttgcg caggtaacgt
    55261 cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt tctggcttca gcgttgtaag
    55321 aaacgctaac cacgcaggtg cctgacgctg ttgccagtat tgcccccaca catctgcgcc
    55381 aatatgcaac cgctgcggcg caaaatagag cccacaggaa cgtaccagcg cggtatcgcg
    55441 cgttatgctc tccccagcct gacaacaata aacctcttcc ggggagtttc cgcgacactg
    55501 gtagggctgc tccggttgat gggtatcctc cagccaggcg taaacataca gtttccacaa
    55561 actccctaac ggcgtggtga gcgagtcagg taatgctgag actttggtca cgcctgatga
    55621 cgacagctga tacagttgat cgtgctgcgc accgcgcagc accagttgta acggcgtctc
    55681 ctccgcaagc gttggcagag taaccaacgc caatagccag acgattctgc gccagttcac
    55741 ttatttcacc tgcatcccgg tccattcact ccctgccgcg acactttgct gcgcaggtgc
    55801 ataggaacgc acataacgag caggaggcag aacgaattgc cctttttgcg agaagcgcag
    55861 caaatggcgg aaagtgaccg ttcccgtcag ttctttcacc ggcaccatat aggccagttc
    55921 gcccatttca ttacgcgctt tttccagcaa ttgcccctgc tgtttcgcgg cgttgggttt
    55981 attgactgaa atgccccatg ttgtgcgctc aacgtcggct cccggcggga gcggtacttc
    56041 cacctgacca tagcgcagaa ctgcatcctg ctcgcaggta agcgtaattt catcgagata
    56101 cagcgcatcg ctgtcaatct cattgctggt caccggctgc agagtaaagc tcatctcttc
    56161 ttcaccgggg ataagccgat acaactggcg ttcaacggtc accggaatgt tactttgttg
    56221 agccgttttt gccggttcac gccagcggac ctgcacattt tgcggcgata attcgtcacc
    56281 aaaagagaga atgtccggca caccctgacc aacccaacgc cagtcttcgc cccctccagt
    56341 taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa acaactggag gcattgtcgc
    56401 catatatttc gccagccagt tcatggccag cgcgcgctca atagtggatt gctcagcggt
    56461 caaaccatta aaatagcggc cgtctgggta gcgtcaccac cgctattgag cagcacaacc
    56521 gtgtgcgcca gcggctgatt gctgtttgcc gctttatccc acgcatattg ctgaacctgt
    56581 ggcatcaccg tggacttcaa ctgcgctttt ttcagcaacg tcatggttaa gacgtttgcc
    56641 accgcatctg ccagtggaga ttccggtgta tcgaggatca ggctgtcatt gatatcgcgg
    56701 atgtcttcct cagagaaatc ttcatcttta gttccgcgcc gggcgatagc ttcatccccc
    56761 cctttcaaca acgttttgca cggcagattc atctcctgtg cccacgccag caccagcgcc
    56821 cgatgcaata acggcatgtt atccgcctgc tcggcataac tgtcgagcat atgctgccag
    56881 tattccggtt gttgcgttac gccgagcgcc tggctggcct gccaatcggc gtaccatgcc
    56941 catgccgtaa ggaaggcgtc accattgcca tcttcacccc accaggtaaa gcgcgctccg
    57001 ggccccgcca gttgcatcag ccgcagacgg ttatcctgaa tcatctgacg aatgtcgtta
    57061 gcggcggcac tttgatgatc ggcaagcgaa cgccaggcga gactgagcgg gatcagacgg
    57121 ctaccggtgt tgatcacccc accccacggt tcatcgacta acgcatcaag attgttgcgg
    57181 aaaatctcct gcggcgtttc actactttgt agccggatat tactcgcctg ctcgggcaac
    57241 atcagcgcgt tctcgccacc gccgagcatg acatttttct gctgttcaac gggccagctg
    57301 ttgtccacaa aagacagttt tgtgctaatg ctgtcctgca cttgcccatt ttgttgcagt
    57361 tctgcactta acaagccaga ttgctgaata ttctgcgtca gcgaaatata attcgcccct
    57421 ttgtgcagcg tcagcgtctg gcgcatctca gcgcctgcaa atttagtcac cagcgccacc
    57481 ggttcgttat cctgctgact gaagataaac agtcctgccg ccggtttgtc gcccacgcga
    57541 tacaccgttg gcatactcca cttcatgtag agattttttt ccgaacgcag ataagcacgc
    57601 ccctgcccga ccagcccgtc gccgttcatc ccacgcgcgg tgatacgcca gcgggttaac
    57661 gaatcaggca tcaggaacgt gaaatacgct ttgccttgtt tatcggttgt gagtgacggc
    57721 atccatgccg cggtatccac ctcttcacgc cgtggacgtt caagcatttt tactcgccgc
    57781 tcgctgcggt tagtcgcgcc aggcgcaacc ggctcgctgg agagcgcctg gtcatagctg
    57841 ataaacgaca gactggagct ggtacgcaca ttgttacgcc ccagcggata gaaaaatttg
    57901 ccgatattcg gcgcgatttc tggttgcagc gcgtagatca tttcatcgac cacgcctacc
    57961 gttagctgcg cagaaacagg tttacctttc agcgacgagg ttaa
//
LOCUS       DADLXX010000013        54586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLXX010000013 DADLXX010000000
VERSION     DADLXX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.013"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(321..1694)
                     /gene="eutA"
                     /locus_tag="JNX27_000753"
     CDS             complement(321..1694)
                     /gene="eutA"
                     /locus_tag="JNX27_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY7882062.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(1669..2148)
                     /gene="eutP"
                     /locus_tag="JNX27_000754"
     CDS             complement(1669..2148)
                     /gene="eutP"
                     /locus_tag="JNX27_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7882063.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(2161..2496)
                     /gene="eutS"
                     /locus_tag="JNX27_000755"
     CDS             complement(2161..2496)
                     /gene="eutS"
                     /locus_tag="JNX27_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7882064.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(2789..5068)
                     /gene="maeB"
                     /locus_tag="JNX27_000756"
     CDS             complement(2789..5068)
                     /gene="maeB"
                     /locus_tag="JNX27_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7882065.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            5357..6307
                     /gene="tal"
                     /locus_tag="JNX27_000757"
     CDS             5357..6307
                     /gene="tal"
                     /locus_tag="JNX27_000757"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7882066.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            6327..8330
                     /gene="tkt"
                     /locus_tag="JNX27_000758"
     CDS             6327..8330
                     /gene="tkt"
                     /locus_tag="JNX27_000758"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7882067.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATCLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(8433..9440)
                     /locus_tag="JNX27_000759"
     CDS             complement(8433..9440)
                     /locus_tag="JNX27_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7882068.1"
                     /translation="MLPTSLVWAAPAQRAFSDWQVTCNNQNFCVARNTGDHNGLVMTL
                     SRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDKWRISPWLLVTDD
                     TATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVGSETAWIKKGDEP
                     PLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSLDPLRREVNVTAL
                     TDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQETNELELMNATF
                     DEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNWHGPDARPTLWIT
                     R"
     gene            complement(9601..10176)
                     /gene="nudK"
                     /locus_tag="JNX27_000760"
     CDS             complement(9601..10176)
                     /gene="nudK"
                     /locus_tag="JNX27_000760"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7882069.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(10244..12212)
                     /gene="aegA"
                     /locus_tag="JNX27_000761"
                     /pseudo
     CDS             complement(10244..12212)
                     /gene="aegA"
                     /locus_tag="JNX27_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            12418..14135
                     /gene="narQ"
                     /locus_tag="JNX27_000762"
                     /pseudo
     CDS             12418..14135
                     /gene="narQ"
                     /locus_tag="JNX27_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            14337..17450
                     /gene="acrD"
                     /locus_tag="JNX27_000763"
     CDS             14337..17450
                     /gene="acrD"
                     /locus_tag="JNX27_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY7882070.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(17549..17608)
                     /gene="ypfM"
                     /locus_tag="JNX27_000764"
     CDS             complement(17549..17608)
                     /gene="ypfM"
                     /locus_tag="JNX27_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7882071.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            17989..18345
                     /locus_tag="JNX27_000765"
     CDS             17989..18345
                     /locus_tag="JNX27_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7882072.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            18349..19476
                     /gene="dapE"
                     /locus_tag="JNX27_000766"
     CDS             18349..19476
                     /gene="dapE"
                     /locus_tag="JNX27_000766"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7882073.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            19504..19704
                     /locus_tag="JNX27_000767"
     CDS             19504..19704
                     /locus_tag="JNX27_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7882074.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(19814..20512)
                     /gene="ypfH"
                     /locus_tag="JNX27_000768"
     CDS             complement(19814..20512)
                     /gene="ypfH"
                     /locus_tag="JNX27_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7882075.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(20586..22601)
                     /locus_tag="JNX27_000769"
     CDS             complement(20586..22601)
                     /locus_tag="JNX27_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7882076.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(22616..23479)
                     /locus_tag="JNX27_000770"
     CDS             complement(22616..23479)
                     /locus_tag="JNX27_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7882077.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(23647..24360)
                     /gene="purC"
                     /locus_tag="JNX27_000771"
     CDS             complement(23647..24360)
                     /gene="purC"
                     /locus_tag="JNX27_000771"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7882078.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVAHRLGVQLD"
     gene            complement(24573..25607)
                     /gene="bamC"
                     /locus_tag="JNX27_000772"
     CDS             complement(24573..25607)
                     /gene="bamC"
                     /locus_tag="JNX27_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7882079.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(25624..26502)
                     /gene="dapA"
                     /locus_tag="JNX27_000773"
     CDS             complement(25624..26502)
                     /gene="dapA"
                     /locus_tag="JNX27_000773"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7882080.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            26648..27220
                     /locus_tag="JNX27_000774"
     CDS             26648..27220
                     /locus_tag="JNX27_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7882081.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            27220..27690
                     /gene="bcp"
                     /locus_tag="JNX27_000775"
     CDS             27220..27690
                     /gene="bcp"
                     /locus_tag="JNX27_000775"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7882082.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            27943..28560
                     /gene="hyfA"
                     /locus_tag="JNX27_000776"
     CDS             27943..28560
                     /gene="hyfA"
                     /locus_tag="JNX27_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7882083.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            28560..30577
                     /gene="hyfB"
                     /locus_tag="JNX27_000777"
                     /pseudo
     CDS             28560..30577
                     /gene="hyfB"
                     /locus_tag="JNX27_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            30588..31535
                     /gene="hyfC"
                     /locus_tag="JNX27_000778"
     CDS             30588..31535
                     /gene="hyfC"
                     /locus_tag="JNX27_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7882084.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            31552..32991
                     /locus_tag="JNX27_000779"
     CDS             31552..32991
                     /locus_tag="JNX27_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY7882085.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            33003..33653
                     /gene="hyfE"
                     /locus_tag="JNX27_000780"
                     /pseudo
     CDS             33003..33653
                     /gene="hyfE"
                     /locus_tag="JNX27_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            33658..35238
                     /gene="hyfF"
                     /locus_tag="JNX27_000781"
     CDS             33658..35238
                     /gene="hyfF"
                     /locus_tag="JNX27_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7882086.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            35228..36942
                     /gene="hyfG"
                     /locus_tag="JNX27_000782"
                     /pseudo
     CDS             35228..36942
                     /gene="hyfG"
                     /locus_tag="JNX27_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            36952..37497
                     /gene="hyfH"
                     /locus_tag="JNX27_000783"
     CDS             36952..37497
                     /gene="hyfH"
                     /locus_tag="JNX27_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7882087.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            37494..38252
                     /gene="hyfI"
                     /locus_tag="JNX27_000784"
     CDS             37494..38252
                     /gene="hyfI"
                     /locus_tag="JNX27_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7882088.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFRDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            38245..38658
                     /gene="hyfJ"
                     /locus_tag="JNX27_000785"
     CDS             38245..38658
                     /gene="hyfJ"
                     /locus_tag="JNX27_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY7882089.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            38688..40700
                     /locus_tag="JNX27_000786"
                     /pseudo
     CDS             38688..40700
                     /locus_tag="JNX27_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            40722..41570
                     /locus_tag="JNX27_000787"
                     /pseudo
     CDS             40722..41570
                     /locus_tag="JNX27_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(41608..42669)
                     /locus_tag="JNX27_000788"
     CDS             complement(41608..42669)
                     /locus_tag="JNX27_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7882090.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            42882..44345
                     /gene="bepA"
                     /locus_tag="JNX27_000789"
     CDS             42882..44345
                     /gene="bepA"
                     /locus_tag="JNX27_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7882091.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            44366..44725
                     /gene="arsC"
                     /locus_tag="JNX27_000790"
     CDS             44366..44725
                     /gene="arsC"
                     /locus_tag="JNX27_000790"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7882092.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(44858..45604)
                     /gene="hda"
                     /locus_tag="JNX27_000791"
     CDS             complement(44858..45604)
                     /gene="hda"
                     /locus_tag="JNX27_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7882093.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(45654..46943)
                     /gene="uraA"
                     /locus_tag="JNX27_000792"
     CDS             complement(45654..46943)
                     /gene="uraA"
                     /locus_tag="JNX27_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7882094.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(47029..47655)
                     /gene="upp"
                     /locus_tag="JNX27_000793"
     CDS             complement(47029..47655)
                     /gene="upp"
                     /locus_tag="JNX27_000793"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7882095.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            47980..49017
                     /gene="purM"
                     /locus_tag="JNX27_000794"
     CDS             47980..49017
                     /gene="purM"
                     /locus_tag="JNX27_000794"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7882096.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            49017..49655
                     /gene="purN"
                     /locus_tag="JNX27_000795"
     CDS             49017..49655
                     /gene="purN"
                     /locus_tag="JNX27_000795"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7882097.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            49827..51893
                     /gene="ppk1"
                     /locus_tag="JNX27_000796"
     CDS             49827..51893
                     /gene="ppk1"
                     /locus_tag="JNX27_000796"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7882098.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            51898..53439
                     /gene="ppx"
                     /locus_tag="JNX27_000797"
     CDS             51898..53439
                     /gene="ppx"
                     /locus_tag="JNX27_000797"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7882099.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(53478..>54586)
                     /gene="pdeF"
                     /locus_tag="JNX27_000798"
     CDS             complement(53478..>54586)
                     /gene="pdeF"
                     /locus_tag="JNX27_000798"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7882100.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12725 a        14463 c        14142 g        13256 t
ORIGIN      
        1 aagcgaaaat ttattgtgcg gtcagactaa caaacttgcg taccacccga agaacatgcg
       61 aagatttaag tattctttct tttttggttt ttatttttca ttttctcttt tcttctcttt
      121 ccggttctct tctgttctgg ttttggtttt ttccttttta tttttattct ttcttcaggg
      181 ttatgctaaa acaccaaaac atttttcacc acaaaagcat ccaatcatct tgccagacca
      241 atcaccttaa ccggatacac tgccggatat aatcccacta acacccagcc accaagcacc
      301 atacctgata actaccgtat tcaggaagga aatgcgagtg atttcaccgt caccggcaca
      361 accgatccgc caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg gacaatcact
      421 tcgtcaatga ctgccaacgg gagttgttgt agctgtgggc gcaacaacat gcccagagct
      481 ttaccaaagt cctgcccggc caccaccagc aggggatgcg gattcggaaa acgcgcgacg
      541 aaatcgacca gcgcgttgat gaccgtcagt accgcggcgt aacgcacagg cagcgaggcg
      601 ggaagcgcca gcacgtacac gtcagttttg ggatcaagat ccagctgaag cagcgcctgt
      661 tgccaggcac tcaccagatc cgtttcatca atcgggatcg ccaccggcaa attgcgcagc
      721 ggcagttgta cgccctccag ccagattgtg ctgccagaga gcgaaagggt atgtgcaccc
      781 gcgccaatca ccgtggcgca tacggtttgc gccggaaact gcacattcat ctcacgcagg
      841 cgcggatggt catgcagcgc cgttgccaga agtgggccaa tatcggcaaa acagaacggg
      901 tcggcgggct ggtggcgata acattcgccc acgcctccag aaagcgtaat gatttcgggc
      961 gtaacacctg cgggcagcaa accggtttgc atcaatgcct gcgcgagcgg cgaaagcgtt
     1021 ccgtcaatca cttcgacaat cagcgccgcc atccgccggg taacctgcac cagctgcgcg
     1081 ccggtcagcg aacgggcgtc agtgcctgca ccgaagcact catccacaat catctgcccc
     1141 ggtttatgag cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg
     1201 ttgagacagg cagtgccgct gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca
     1261 ccgccgatat cgatattcag tacccgacac agccgttgtt cagaaagggt ttgcgccccg
     1321 gctccgtgac cggcgatcac ggattcgagg tgcggcccgg cgctggcgac gacaaaatcg
     1381 cccagcgact gtttttcctt catcactttt tccatcagct gtgcgaccag gctttcggta
     1441 aactggcctt ccggcagctg ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg
     1501 tcggctggca cgctggcggg cacggacgcc gttaccggga tcgattcatc acattcggtg
     1561 atggtaaaac ccagtagatc cgacacttca cgggcttccg gggtaataat gctggcgcgc
     1621 agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg
     1681 atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg
     1741 ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga aacccgtctc
     1801 aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac
     1861 gacgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg
     1921 gtcatttgcg ccatggacat agatcagcat gtctacatcc tgtagcgtgg taattaaggc
     1981 gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc ccttatcatt
     2041 aaattccacg gcctgtgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa
     2101 cgttgttttt cctgccccga cgctacccac aaacgcaatt cgtttcatga atgcctcgta
     2161 ttaacttttg gtcatttcac aaagcgtata atttaataag cgacctaaac cgctgacggt
     2221 ttgtgataac gcttcttcta ctgcgcccac cgagccataa atcaccaatg caccgctgaa
     2281 gcggtcgaga aaaccgatat gcacatcagc ggctttcagc gctaaatcac cggcgatcat
     2341 cgccgtttcg ccgggagtta gcgtcataat gccaattgcg cccgcatccg gaacgccgat
     2401 tttcttcgcc agttcctcac cgggatgcgc gatgagatgc gccagtgtga cctgtttgcc
     2461 gggcacaaat tcctgaatga tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt
     2521 cgtgtatcgt gacaattttt cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa
     2581 ggtgaggtag atcactaaga atggagagat atttactgtt atgaataaac tttcatatgc
     2641 gacgcatgaa tataaataaa ataatatcga gatattttat atactgattt ttttattatt
     2701 cgcaaactca gtaataagct ttgctaatat atttcaggaa aaccgcttac cctgaatatt
     2761 cagggtaagc gtgagagtta agaatgaatt acagcggttg ggtttgcgct tcaaccacgg
     2821 ccagcgccac catattgacg atacgacgca ccgatgcgat cggcgttaac acgtgaaccg
     2881 gtttcgccac gcccatcagc accggaccga cagtcacacc ttccgagctg gaaacgcgca
     2941 gtaagttgta actaatgcgg gcagcttcca tgttcggcat caccagaata ttggcagaac
     3001 ctttcaaagg gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc
     3061 cgtgcatttc accatcaatc atcagatctg gcgcacgatc cctgaccagt tccagcgcct
     3121 gacgcatttt gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacagag
     3181 caacgcgcgg ctcaatacca aaacgacgga cagtttctgc cgccatcaag gtgatctccg
     3241 ccagctcttc tgcgtctggt tcgtcattga cgtaggtatc ggcaacaaag gtgttaccac
     3301 tcggcagcag cagcgcgttc attgcacctg cggtgtgaac gccatcgcga taaccaaaga
     3361 catttttcac cacgctaaaa tgttcatgat aatcgcccac cgtaccgcag atcattgcat
     3421 cggcttcccc acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgcac
     3481 gctgcgcctg ttcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt
     3541 actctttaaa gcgcggatcg gattcgttat tgacgatttc aaaatcaacg cccgctttga
     3601 tctgcaagcc cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg
     3661 gtttcgccag tcccagcgtt accagttcct gagtcgcatg cagaacgcgc gcctcttccc
     3721 cttccggcag aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa
     3781 acaggttggt tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa
     3841 tcggacgcgt cgccacgccc gactccatcg ccgcttttgc gaccgcagga gcgatcttaa
     3901 cgatcaagcg cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct
     3961 gaacgccata cgctgaagcc accacttcac tctgttctgc atgggccagt tctgcaatcg
     4021 cgcgtaccgc cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tcaagcgcgc
     4081 cacggaagat gaacgggaag caaagaacgt tgttcacctg gttcgggtag tcggaacggc
     4141 cggtacagat gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt
     4201 ccgggttcgc cagcgccagg atcattggtg cccgagccat tttcttcacc atttcctggg
     4261 tcagcacttt cgggccggaa cagcccagga aaatatccgc accttcaatc acatcatcga
     4321 gggtacgttt gccgtcatcc accaccgcat atgcggcttt ggtttccgcc atatttggct
     4381 cacgcccctg atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagcc
     4441 ccagcgctac cagcaggttc atacaggcga ttgctgcggc acccgcgccg gaaaccacca
     4501 tccgcacgtc ggagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc
     4561 tgatgattgc cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca
     4621 gtttctgttc aatatagaaa cattctggcg ctttaatgtc ttcgaggttg atgccgccga
     4681 aggtcggttc gagcgcggcg acgacttcaa taaatttgtc cgggtcgagt tcatcaactt
     4741 caatgtcaaa tacatcaatc ccagcgaatt tcttaaatag aacgcccttg ccttccatca
     4801 ccggtttgcc tgccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga
     4861 tcaccgcaac caggttaccg cgcgcggtat atttgtaggc ttttagcggg tctttttcga
     4921 tttcaagaca aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg
     4981 ccagaggctt ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa
     5041 gtgcactttg ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat
     5101 aaagtgtgtg ggcaacagcc cctgcccaca acgtggcgca cattattacc ctgccggagt
     5161 ctacagactt tgagcaagtc caaactctca ccattaatat aatgttttgg taacaatcct
     5221 ataacactga tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg
     5281 atgcgtttag cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt
     5341 tcaaggagta tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc
     5401 agacagcggc gatattgagt ccattcgcca ttatcatccc ctggatgcca ccaccaatcc
     5461 ttcgctgtta ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat
     5521 cgcctggggt aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa
     5581 actggcggtc aatttcggtg ctgaaattct caaaatcgta cccggttgcg tgtcaacaga
     5641 agttgatgca cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt
     5701 ggacttgtat cagcaacaag gcgttgagaa atcacgcatt ttgatcaaac tggcttcgac
     5761 ctgggaagga attcgcgcgg ccgaagagct ggaaaaagaa ggtattaact gcaacctgac
     5821 gctgctgttt tcttttgccc aggcgcgggc ctgtgcggaa gcaggcgttt ttctgatttc
     5881 gccgtttgta gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt
     5941 ggtggaagaa gatccgggcg ttaaatcggt gcgcaatatc tacgactact ataagcaaca
     6001 ccactatgaa accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt
     6061 aaccggctgc gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt
     6121 ttctccagtg gtacgtaaat taatcccgcc ttctcagacg ttcccacgcc cagcacccat
     6181 gagcgaagcg gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc
     6241 tgaaggcatt cgtctgttcg ctgttgatca acgcaaactg gaagatcttc ttgccgccaa
     6301 actataaacc agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg
     6361 cactcagtat ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca
     6421 tggctgatat tgccgaagtg ctgtggaacg attttcttaa acataatcct accgacccaa
     6481 cctggtatga tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca
     6541 gtttgctaca tctgaccggt tacgacctgc cgctggaaga actgaagaac ttccgtcagt
     6601 tgcattcgaa aaccccaggc cacccggaga ttggctatac gccaggcgtt gaaaccacca
     6661 ccggcccgct tggacaaggt ttggcgaacg ccgtcgggct ggcgatagca gagcgtacac
     6721 tggcggcgca gtttaaccag ccagaccatg agatcgtcga tcacttcacc tatgtgttta
     6781 tgggcgacgg ctgcctgatg gaaggtattt cccacgaagt ctgttcgctg gcaggcacgc
     6841 tgggactagg caagctgatt ggtttttacg atcacaacgg tatttccatc gacggtgaaa
     6901 cagaaggctg gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga
     6961 tccatgaaat cgacggtcac gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa
     7021 gcgtgaaaga taagccgtcg ctgattatct gccgtacggt gattggcttt ggttcgccga
     7081 ataaagcagg taaggaagag gcgcacggcg caccactggg ggaagaagaa gtggcgctgg
     7141 cacggcaaaa actgggctgg caccatccgc catttgagat ccctaaagag atttatcacg
     7201 cctgggatgc ccgtgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg
     7261 cctataaaaa ggctcatccg caactggcag aagagtttac ccgacggatg agcggtggtt
     7321 taccgaagga ctgggagaaa acgactcaga aatatatcaa tgagttacag gcaaatccgg
     7381 cgaaaatcgc tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccgatgctgc
     7441 ctgagttgct cggcggttcg gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt
     7501 ctgtttcgct gaaggaagat ccggcgggca actacattca ctacggggtg cgtgaatttg
     7561 gcatgaccgc tatcgccaac ggcatcgcgc accacggcgg ctttgtgccg tataccgcga
     7621 cgtgcctgat gtttgttgaa tacgcccgta acgccgcgcg gatggcggca ctgatgaaag
     7681 cgcggcagat tatggtttat acccacgact caattggcct gggcgaagat ggtccgacgc
     7741 accaggctgt tgagcaactg gccagcctgc gcttaacgcc aaatttcagc acctggcgac
     7801 cgtgcgatca ggtggaagcg gcggtgggct ggaagctggc ggttgagcgc cacaacggac
     7861 cgacggcact gatcctctca aggcagaatc tggcccaggt ggaacgtacg ccggatcagg
     7921 ttaaagagat tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta
     7981 ttctgattgc caccggttca gagatggaaa ttaccctgca agcggcagag aaattagcag
     8041 gagaaggtcg caatgtacgc gtagtttccc tgccctcgac cgatattttc gacgcccagg
     8101 atgaggaata tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag
     8161 caggtattgc cgattactgg tacaagtatg ttggtctgaa aggggcaatt gtcgggatga
     8221 cgggttacgg ggaatctgct ccggcggata agctgttccc gttctttggc tttaccgccg
     8281 agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc
     8341 cggatgctgt atgttcccct caccctaacc ctctccccag aagggcgagg ggacggtccg
     8401 tgcgcttttt agggccgcat ctgcgatttt gtttaacggg tgatccacaa cgtgggcctg
     8461 gcgtctggcc catgccagtt atcgcaggta ggttctgcgg cgtaacgcac caggcgaaaa
     8521 cgctgaccgt caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc
     8581 gctaaggtca ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca
     8641 ttcgtctcct gaccgttgtt gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc
     8701 tttttacgcg acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagcta
     8761 atcatcatca gtgctttatc atcggtcagc gcagtgacat tcacctcacg acgtaacgga
     8821 tcaagcgagc agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttacgt
     8881 tcttcgagtg agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc
     8941 gcgggcggta cgctgagagg cggttcgtcc cctttcttga tccacgcggt ttcactgcca
     9001 acgcgctttt gctgagcatc aataaacaac aacgccgctt ttaagccgct cagagaaatg
     9061 gtctgattgc cgtcgcgtaa ggtgatcgcc ttcccttcct gaattatctg caaaaacgcg
     9121 gtgatggttg ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca
     9181 cttaatgcca acggctcacc atctaatagc agccgtggcg ctatctcccc ttcagacgca
     9241 tccggcgact tcaacccacc gcgctcaata cgtaaaacgg catcggtatg cgccccagcg
     9301 ctgcggctca gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacacaaaaa
     9361 ttttgattat tacaggtgac ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac
     9421 accaaagacg tcggcaacaa agcgaaaaaa agagaaggaa aatgcgatag cgcatggacg
     9481 gtacgacccc agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc
     9541 ggctcaatcg gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgctatttt
     9601 tcagtccatt aaatgtgaca tttgcaaata gttaagcaat aacaccgtct taccgtcacg
     9661 tatctcgccg gttttgatca tctccagcgc ctggctgaac ggcagctcga gcacttcaat
     9721 atcttcatct tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat
     9781 aaaaaagtgg attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg
     9841 cacttcgccc acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg
     9901 ttcgtcgtta tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt
     9961 aacccaggta gcgacacgga actgacgaat cagaaccacg ctctttttct tcgcgttgta
    10021 caggaggatc gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataacttc
    10081 accatctttg cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag
    10141 aattttgtct ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa
    10201 caattatatt acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg
    10261 attttacccc cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg
    10321 taaccaccag atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg
    10381 tctggtaacg gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta
    10441 caccgtgcga ctccagccac ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg
    10501 catcggctgg catgacaaat tcactgccct caatcggtac aggtcgccga cgcccctggg
    10561 catccggctc gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat
    10621 tcagctcaag cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt
    10681 tcttcacctc tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga
    10741 cgttactcgc gccgtggcgc agcgcagtac gcacacagtc catcgcggtg tcgccgccgc
    10801 ccagtaccac gacgttaagt ccggcggtat tgataaacgg ctcttccggt agctcttcga
    10861 gccccatcac ctgtttagtg ttggcaatga ggaacggcag cgcgtcataa acgcccggcg
    10921 tatcttcatt gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg
    10981 cgtcgtattg ttccaaaagc gaatccaaag agacatcttt acccacttca caattgagtt
    11041 cgaagtgaat ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca
    11101 gtttgaaaga aggaatgccg aaagtgagca agccaccgat ttctggatgg cgatcgtaca
    11161 ccgtcaccgc cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacctg
    11221 ccccgataat cgccacccgc ttgtccactt tggtgacatg acttaagtca ggacgccaac
    11281 ctttcgccaa cgcctgatct gaaatatagc gttcaatgtt gccgatagtt accgcgccgt
    11341 gctcatcgcg aatagtacag gcaccttcac acaaacggtc ttgcggacaa acgcgtccgg
    11401 taatttccgg cagggtgttg gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg
    11461 ctttcaccag ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa
    11521 cgctgtgctc gccgaactta aggcagcgcg aggcttcccg ttgtgcctgg tcgcagataa
    11581 atttcatcaa aaccggtttt gcgcgcttca atcgccagtt tatccggctc gccacgcgcg
    11641 ggcgttgcct gcatttgttc gactttactc attaccggca tttcttgtgc agcggtactg
    11701 gcatgccacg gttgatgctc ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg
    11761 gagagtgcga cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc gacacaggca
    11821 ggaccgtttt cgcgccctgc acaaaggtcg catttatgcg ccgtggcttt tacttttcct
    11881 gccgcgacgg gtgtcaggac gatttgcatc gtaccaaacg gacaggccac cacgcaggat
    11941 ttacagccaa tacacttttg ctgattgacc tgaatgctgt catcaacgtg actgattgca
    12001 ccattagggc agctacgggc gcagggcgca tcttcacaat ggtgacaggt cactgcacta
    12061 cgttgctgct gatgtttgat aaccgtaatt cggggatgaa aatggtgttg gctcaggaca
    12121 tgttgctcat cattgtgagc catgacacag gcgatttcac aagcatgaca acccagacat
    12181 tgctgactgt tggccataat aaaacgattc ataacgacct tcttttttgg ttgtaaaaac
    12241 cttattcttt atatgagtgt tgttattacc cgacttacag gggaaccggc aatgttcatg
    12301 tgcccagaat aagtaactat ttcgctataa actgtggcag atcaaataat cccatcactg
    12361 actaaattgc gtttcagcga actggaacat taatgatttt ttgtggagaa gacgcgtgtg
    12421 attgttaaac gccccgtctc ggccagtctg gcccgggcct ttttttacat tgtgctgctg
    12481 tcgattcttt ccacgggtat cgctctgcta actctggcga gcagtttgcg cgacgctgag
    12541 gctatcaata ttgccggatc gctgcgtatg cagagttacc gcctgggcta cgacttgcaa
    12601 agtggcagtc cacaactcaa tgcacatcgc cagttgtttc agcaggcact gcattcaccg
    12661 gtattaacca acctcaacgt ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat
    12721 ctgaatgcca actggctgga gatgaataat cggctcagca agggcgattt gccgtggtat
    12781 caggccaata ttaataatta tgttaatcag atagacctgt tcgtgctggc tttacagcac
    12841 tacgctgaac gcaaaatgct gctggtggtg gcgatttccc tggctggcgg tatcggtatt
    12901 ttcacgctgg tcttttttac tttgcgccgc atacgccatc aggtggttgc cccgctgaat
    12961 cagctggtta ccgccagtca gcgtattgaa cacgggcagt tcgactcgcc gccgctggat
    13021 accagtctgc cgaatgagct tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag
    13081 ctgcataaat tgtacctttc gctggaggcg tcagtagaag aaaagacccg cgatctccac
    13141 gaggccaagc gtcgtctgga ggtgttgtat cagtgcagtg ttcccaggcg ctgaacacca
    13201 gccagattga tgtgcattgt ttccgccata ttttgcagat tgttcgcgac aatgaagcag
    13261 ctgaatatct ggagctgaac gttggggata actggcggat tagcgaaggg aagccaaacc
    13321 ctgagcagcc aatgcagatt ttaccggtga caatgcaaga gacggtttac ggcgaactgc
    13381 actggcaaaa tagtcacgtt tcatcatcag aaccgctgct taacagtgtt tcatcgatgc
    13441 tgggacgcgg tttgtacttt aatcaggcgc agaagcattt tcagcagtta ttgttgatgg
    13501 aagaacgtgc gaccatcgcc cgcgaattgc acgactcgct ggctcaggtg ctttcttact
    13561 tacgtatcca gttgacgtta ctgaagcgtt cgataccgga agataacgcc accgcacaaa
    13621 gtatcatggc cgatttttcc caggcgttga atgatgctta tcggcagtta cgcgagctgt
    13681 tgactacttt ccgcctgacg ctgcagcagg cggatctccc ctccgcgttg agggaaatgc
    13741 tggatacgtt acaaaatcaa accagcgcta aactgaccct cgactgccgt ctgccaaccc
    13801 tggcactgga tgcgcaaatg caggtgcatt tgttgcaaat tattcgcgaa gcggggctga
    13861 atgcgatgaa gcacgccaac gccagcgaaa tcgccatcag ttgcgtcacc gcgccggacg
    13921 gcaatcacac ggtttatatc cgtgataacg ggattggtat cggtgaaccg aaagaacccg
    13981 aaggtcatta tggactgaat atcatgcgcg aacgcgcgga acggctgggc gggacgctga
    14041 ctttttcaca accttccggc ggcggcacgt tagtgagtat tagctttcgc tccgcagagg
    14101 gtgaggggaa ggggaacacg ccggctgagg tgtaaataat taccctcaca ccggccctct
    14161 ccctttaaag gcgagaggga aaaacgtgcc catattgacc tgtaagcgag tgtgtcagca
    14221 atctcatgat aatttacatt aactcctttt tttctccacg attggctcgt accttgccgc
    14281 tacagtgaag caagtcaagc ctacaacaat acgcagaaac acgaggtcct cttttaatgg
    14341 cgaatttctt tattgatcgc cccatttttg cctgggtgct ggcaatcctg ttgtgtctga
    14401 caggtaccct ggcgattttt tcattgcccg ttgaacaata ccccgatctc gcgccaccga
    14461 atgtgcgagt gaccgctaac tatcccggcg catcggccca gacgctggaa aacaccgtga
    14521 cccaggttat cgagcaaaat atgaccggcc tcgataatct gatgtatatg tcatctcaga
    14581 gcagtggcac cggtcaggca tctgtcactt taagttttaa agcaggcacc gatccggacg
    14641 aagccgtgca gcaagtacaa aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg
    14701 tgcaaaatca gggcgtgacg gtgcgtaaaa ccggcgatac caacattctg accattgcct
    14761 tcgtctctac cgatggctcg atggataaac aggatattgc tgattatgtt gccagtaata
    14821 ttcaggaccc gttaagccgc gtgaatggcg tcggggatat cgatgcctat ggttcgcaat
    14881 attccatgcg tatctggctg gacccggcga aactcaacag tttccagatg acggctaaag
    14941 atgtcactga tgccattgag tcacagaacg cgcagattgc ggttgggcaa cttggtggta
    15001 caccttccgt cgataagcag gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa
    15061 caccagaaca gttccgcgat atcaccttgc gggtgaatca ggacggctca gaggtaaggc
    15121 tgggcgatgt cgccaccgtc gaaatggggg cggagaaata cgattatctt agccgcttca
    15181 atggtaagcc agcctccggg ttgggggtaa aactggcctc cggcgcaaac gaaatggcga
    15241 cagcggagct ggtgctcaat cgtctcgacg agctggcgca gtatttcccg cacggactgg
    15301 aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa gacgtggtga
    15361 aaacgctgct ggaagctatc gctctggttt tcctcgttat gtatctgttc ctgcaaaact
    15421 tccgcgccac gctgataccc actatcgccg tgccggtggt gttgatggga accttctccg
    15481 tactttacgc cttcggttac agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga
    15541 tcggtctgct ggtggatgac gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg
    15601 aggaaggact cactcctcgc gaagccacac gtaaatcgat ggggcagatc cagggggcac
    15661 tggttgggat tgcgatggtg ctttcggcgg tatttgtacc aatggccttc ttcggcggca
    15721 ccaccggtgc catctatcgc cagttctcta ttaccattgt tgcggcgatg gtgctgtcag
    15781 tactggtagc gatgatcctc actccggctc tgtgtgccac gctacttaag ccactgaaaa
    15841 aaggtgagca tcacgggcaa aaaggttttt ttgcctggtt taaccagatg tttaaccgca
    15901 acgccgaacg ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg cgctggattg
    15961 tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt
    16021 tcttaccgct ggaagaccgg ggcatgttta ctacctcggt acaattgccc agcggttcaa
    16081 cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa atattacttc atccatgaaa
    16141 aagacaacat catgtcggtg tttgccaccg ttggttctgg ccctgggggt aacgggcaaa
    16201 acgtggcgag aatgtttatc cgcctgaaag actggagcga acgcgacagt aagaccggca
    16261 cctcgtttgc cattatcgag cgtgcaacga aggcgtttaa ccaaattaaa gaagctcgcg
    16321 ttatcgccag cagcccacca gcaattagcg gtcttggtag ttctgcagat tttgatatgg
    16381 agttgcagga ccacgctgga gcgggtcacg atgcgctgat ggcagcacgt aatcagttgc
    16441 tggcgctggc ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc ctcgatgaca
    16501 gtccgcagtt gcagattgat atcgaccagc gtaaagctca ggcgctgggc gttgatatcg
    16561 acgatattaa cgacacactg caaaccgcct ggggttcgag ctatgtgaat gactttatgg
    16621 atcgcggtcg cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc atgctgccag
    16681 atgacatcaa tctctggtat gtccgaaata aagatggcgg catggtgccc ttctctgctt
    16741 tcgcgacctc acgctgggaa acaggctcgc cgcgtctgga acgctataac agttattctg
    16801 cggttgagat tgttggggaa gccgcaccgg gggtcagtac cggtacggcg atggatatta
    16861 tggaatcgtt agtgaagcag ctgccaaacg gctttggtct ggagtggacg gcgatgtcgt
    16921 atcaggagcg gctttccggc acgcaggctc cggcgctgta cgccatttcc ttgctggtgg
    16981 tattcctgtg tctggcggca ttgtatgaaa gctggtcggt accgttctcg gtaatgctgg
    17041 tcgtgccgct gggggtaatc ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg
    17101 acgtttactt ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa aacgccatcc
    17161 tgatcgtcga gtttgctaac gagatgaacc aaaaaggcca cgacctgttt gaagcgacgc
    17221 tccacgcctg ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca tttatcttcg
    17281 gcgtattgcc aatggcaacc agcacgggtg ccggttccgg tggtcagcat gcggtgggta
    17341 ctggcgtaat gggcgggatg atttcagcta ctattctggc tatttacttc gtgccgctgt
    17401 tctttgtgct ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa
    17461 aggcgacatg ccaatgtgtc gcctttttca actttccgat taagaacctg ctcagcgggt
    17521 tcttgctgtt tatactttgt ctcaggaatt acttacgaag cataacttcg ataaagtctt
    17581 tccagttccc cagttcacgt tcaatcataa caacctctct tataattatg ggtattctac
    17641 ggaaaaaata taccgtggtg aagctaattt actcgattgc tgcgatgact acctccgggg
    17701 gacaaacctt atgtaaatac tatggtcctg cggtgataat ttgtatgtga tacacagcaa
    17761 catttcgaga tattcatacg gcatctaata cttatttaat tctggttaaa atacagacag
    17821 ataacaagat gaatattctt aatgtttacg ttaaaaatgt ttaatattat ttaatagttg
    17881 ttaatttgaa tacttcgata atgttatatt ttctgataat catttgcagg caaaatgttt
    17941 tcacccttaa atgagtattt attctcataa atcgaaaaag gattcattat ggttacactt
    18001 tacggcatca aaaattgtga caccattaaa aaggctcgcc gttggctgga agccaataac
    18061 atcgactatc gttttcatga ttaccgcgtc gatgggctgg acagcgaatt attgaacggt
    18121 tttatcaacg aattaggctg ggaagcgtta ctcaacaccc gtggtacaac ctggcgtaaa
    18181 ctggacgaaa ctacccgcaa taaaatcacc gatgcggcct ctgcggcggc attaatgact
    18241 gaaatgcctg caattatcaa acgtccattg ctctacgcgc ctggtaagcc tatgctgctg
    18301 ggtttcagtg attccagtta tcagcaattt ttccatgagg tgtagtctat gtcgtgcccg
    18361 gttattgagc tgacacaaca gcttattcgc cgcccttccc tgagtcctga tgacgcagga
    18421 tgtcaggctt tgttgattga acgtttgcag gcgatcggtt ttaccgttga acgcatggat
    18481 tttgccgata cgcagaattt ttgggcatgg cgtgggcaag gtgaaacgtt agcctttgcc
    18541 gggcataccg acgtggtgcc gcctggcgac gccgatcgtt ggatcaatcc gccgtttgaa
    18601 cccaccattc gtgacggcat gttattcggg cgcggtgcgg cagatatgaa aggctcgctg
    18661 gcggcgatgg tggtagcagc agaacgtttt gtcgcacaac atcccaacca tacggggcga
    18721 ctggcatttc tgatcacttc tgatgaagaa gccagtgccc acaacggtac ggtaaaagtc
    18781 gtcgaggcgt taatggcacg taatgagcgt ctcgattact gcctggtcgg cgaaccgtcg
    18841 agtatcgaag tggtaggtga tgtggtgaaa aatggtcgtc gcggatcgtt aacctgcaac
    18901 ctcaccattc atggcgttca ggggcatgtt gcctacccac atctggctga caatccggta
    18961 catcgcgcag cacctttcct taatgaatta gtggctattg agtgggatca gggcaatgaa
    19021 ttcttcccgg cgaccagtat gcagattgcc aatattcagg cgggaacggg cagtaacaat
    19081 gttattccgg gtgaactgtt tgtgcagttt aacttccgct tcagcaccga actgactgat
    19141 gagatgatca aagcgcaggt acttgccctg cttgaaaaac atcaactgcg ctatacggtg
    19201 gattggtggc tttccgggca gccatttttg accgcgcgcg gtaaactggt ggatgcggtc
    19261 gttaacgcgg ttgagcacta taatgaaatt aaaccgcagc tactgaccac aggcggaacg
    19321 tccgacgggc gctttattgc tcgcatgggg gcgcaggtgg tggaactcgg gccggtcaat
    19381 gccactattc ataaaattaa tgaatgtgtg aacgctgccg acctgcagct acttgcccgt
    19441 atgtatcaac gtatcatgga acagctcgtc gcgtgatgag tggttctgca agaggaaata
    19501 agcatggact ggctggctaa atattggtgg attctggtga ttgtcttttt ggtcggcgtc
    19561 ctgctgaacg taattaaaga tctcaagcgc gtcgaccata agaaatttct cgccaacaag
    19621 ccggagcttc ccccgcatcg tgatttcaac gataagtggg acgatgacga cgactggccg
    19681 aaaaaggatc aaccgaagaa gtaagctgag tgctcaaccg taggccggat aaggcgttaa
    19741 cgccgcatcc ggcaatggtg aacgatgcct gatgcgacgc tcacgcgtct tatcaggcca
    19801 ggtaatctgt cttttacatc atttcaatca catcatcatc gccaggctta ccgccactta
    19861 gcgcttggtc gaaataatgt ttcggaatgg tataacgcaa gtgatccagg gcaaattgca
    19921 tactgcgatt gtcgatcgca tggcctaaat cctccacgat atccagcgtc acgtcaccac
    19981 cggcactgat taacgcttcc tgcgccgcaa ctgcatgcgc cagatcgata accggatctt
    20041 caccaccatg aatcaagtga attgtcgtgg ctgtagacgc ggtctccggc aggctcgcat
    20101 agcgtccgtt aaaggcaatg acgcgcgaag caaggcccgg ttcggcttta atgctctcca
    20161 gcgccataat cgcgccttgt gagaaaccaa ttagcgccgt ggcgttagcc cctactccgc
    20221 tctgtttctg ccagtagcgt accgtttcaa taaacgtcgg catgatggca tccacacgcg
    20281 cctggcgatt atcttccgta ataccctgca ctgaaaacca ctgacgcccc gccggattac
    20341 cgctcggctc cgcgccaccg acactcacca ccagcgcatc aggaaacagc ggtgcaaacc
    20401 agctgcctat ttcccccatt gccaccgggt tatccccgac accgtggaaa agcagtaaca
    20461 actgttgtgc aggtttatcc gggctttgaa caacaaaatg gtcatgtttc atggcgaact
    20521 ccttaactga tgtcattaat tttacgccgc tgactcacaa tgaccatggg agaaaattga
    20581 aggagtcagt gaaaaaattg ccattgcgtt atgcgatcgc gcagacgctc agtgcgaacc
    20641 tcatttagtg cgaacagcgc ctgcgcggcc tcttcccgct ggcgaaccag taacatcttg
    20701 cggcctgaaa gtttaagtgt ggtacataac tgcgcgtcac tggcgttttt ctgtaaacgc
    20761 ccacgcagcg ccggtaatgc cagttcacta gtttgtagca gacgcaataa gcaacctaac
    20821 gatgttaata gcggacgatg agcgaaagca aaaccggcca gttcaagcca gtcgtcgtca
    20881 gaaaggatgg catcgtttag tggatcaaca ggaagcattt cgccattcca ctgcgcgaga
    20941 gcttgcgcat cgcgacgtaa acggtaatgc tcacgttcag ccagctgttt acccgcatca
    21001 ctcatcggta acagcgccat cgccgtatag caaccgctgc tggcttcccg atgattaccc
    21061 atccgcacca gcacaaaacc gcagcgttgc cagaaacgcc ataactcccc agtgtaacca
    21121 aaactcaccg aaagatagtc gaggtcatgc gtatattgca aagctccagc aataagttgc
    21181 cgccctgtgc cttcccgctg acgagccgga tgaactgcta tccggctaac ccgccttccg
    21241 cgcaatgtcg ccgccagtgg attgctgccg tgcgccgcca gcgactgggc caccagatta
    21301 ccccgcgggc gacgaaaacc tgcccatacc gcctgactga gttcttgaga taatccgccc
    21361 tcatccacca gccacaacgc cccggcaatc tcgttttcgc cagccgcctg taaaaaatgt
    21421 tgccctggtg catccatcat ccggcgtaaa tccagcggcg aggtccggta gtgcgcacca
    21481 gacaataact gataaacctt taacggcgtt tctggctcgc ttcgccataa cgtttgttca
    21541 aatgcggaaa tgacgatatt gccttgtggt gtatgggtga agttttcatc gttaaaaacc
    21601 agtgcctcac taaccatttt ttccagcggg catccctgtg cccagcggat cggctgttgc
    21661 agttcaaaac ggtgtaaatg cggaaagcga gcgcaaaatt tcagcaaaaa accacgtccg
    21721 gtaccttcgt agccctgcac cgtagtggtt aacaacgttc gaggaaaacg cgataccagt
    21781 tgatgcaaca acggcgcagg tatggctgcg gcttcgtcga ccaccagcca gtcggcttgc
    21841 tcatcgctgg ctaacaaggc atccggtgca ataaagcgaa acttctcgcc cgcaaattgt
    21901 gccagtacat ccgttgccgc ttttgcgggc gcggtgacaa tcgcactgcc cgcaatacga
    21961 gaaatgagtt gccctgccaa cgccgactta ccgcgcccac gcgcagccgt taccgctgcc
    22021 acgcccggcg gcatggtcag tagctgctgt aagagttgct gttgttctgg ttgtggtgcg
    22081 ccggtcgcgg ggtgccagtc agtacgggga gtaaaatgcg ccaacgagaa cggctggttt
    22141 tgccgccaga ggatagcgtc gttatccgcc gtaagtacgc gtttgagatg ctggacaaaa
    22201 tgcggcgtcg caatagggtc agggcaatca ctccagcgca gcgagtcggc atcaggttgg
    22261 ttttcccact cttcccatac agggagtaac aaaaccagcc agcttcccgc tttcaacgtt
    22321 ccgctaagtg ctgcaaaggc agcggcatca aagccgtggc gggcgtcgaa taccgcatgc
    22381 cggaactcgc gcccaagtaa agtttgtagc gccgagggtg aacagtggtt ttcagcatct
    22441 agctgcggcg aaatccacag ccagtcgcca ggtaaggcat cacgcaactt aagagcatga
    22501 tcaaaacacc aaccctcttc tccgctcaac accagcaagc ggcagatccc ttcacgtttc
    22561 atttgcgctg ttaatgtgta aagcgcagtc agttcagcca tccctgcccc ggaaattaaa
    22621 tgcttttacc aaaagtattg cattgtgccg gatcgccgct gtcgaaacca cgtttaaacc
    22681 agctgcagcg ttgctgagaa gtgccatgtg tgaaactgtc tggtactact cgcccctgac
    22741 tttgctgttg taaacggtca tcgccgatgg cctgcgccgc attcagcgcc tcttccagat
    22801 cgccagtttc cagaacgcct tgctgctgca tactatgccc ccagacaccg gcaaaacagt
    22861 cggcctggag ttccatacgc acagataagc ggtttacttc cgcctgcgtc gcgttttgtt
    22921 gcagttgacg aactttcggc tcgatgccta acagtttctg cacatgatga ccgacttcat
    22981 gggcgataac gtacccctgg gcaaaatcgc catccgcgcc aagtttgtct ttcatgtcat
    23041 catagaagga gagatcgata taaactgtgc catccgccgg gcaatagaac ggccccataa
    23101 tggactggcc cgccccgcag ccggtgcgcg tcattccacg gtacatgacc agcttcggtt
    23161 gctgataggt cttacccatc ttttcgaact gttgtcccca ggtgtcttcc gtggttgcca
    23221 gaatcaccga ggtgaatttt gcggcttcgt cttcattagg gctaattgac cgcgttgatt
    23281 gttgttgaga aaccggctgc ccggtcatca acccggttaa atcaacgcca tagtagcctg
    23341 caaccagcac gactatcagt aaaatcagcc cgcctttacc gctcggcaga cggaaaccgg
    23401 gaccgcccat tgacggaccg ccagagctgt tgcgcctgtc ttcaacattg tcactttcac
    23461 gtcgcccttg ccaacgcata gatacctcaa caatatattc attatagaaa tgatcgtagg
    23521 tggttcagag gaagattacc acaggaaagc gggatgaaat ggcggaggaa tgagcgttca
    23581 atgcatatat tccgagagcg gttgtcttgc cgtgccagct gcacggcaag atgatgaaca
    23641 gaaaaatcag tccagctgta cacccaggcg gtgggcgacg gcttcatagg cttcgatcag
    23701 gccaccgaga ctctggcgga aacggtcttt gtccattttc tccagtgttt ctttgtccca
    23761 caggcggctg ccgtccgggg agaactcatc gcccagtacc acttcacctt tgtacagacc
    23821 aaactccagc ttgaagtcga ccagaatcag accagcatca tcgaacagtt ttttcagcac
    23881 gtcgttcgct ttgtaggtca gctctttcat acgcgccagg ttctctttgc tcacccagcc
    23941 aaaggtttcg cagtaagatt cgttgaccat cggatcgtgc atggcatcgt ttttcaggaa
    24001 cagatcgaac agcggcgggt tcagctcaat accttcttcg attccaagac gtttcaccag
    24061 agagccagca gcacggttac gcacgacaca ctcaaccggc accatatcca gctttttcac
    24121 cagacattcg gtatcggaga gcagacgctc catttgagtc gggatacccg cttcagccag
    24181 tttgctcata atgaagtagt tgaacttgtt gttcaccata cctttgcgat caaactgctc
    24241 aatgcgcgcg ccatcccctg ctgacgtatc attgcggaat tcgagcacca acaggtccgg
    24301 gttttccgtg ctgtatacgg ttttcgcttt accacgatac aactcagctt gcttttgcat
    24361 ctttatcact cctgggtgtg aattaacgtt ttaaaatctt ttgctgtctg gtgtgccgga
    24421 tgttttttcg gatgcggcgt gaacgcctta tccgaactac gtccgacaaa caaatttcgt
    24481 gcgaattacc gctaaaatcg cgttttcctg ccgacgcaca cgtttgcgta tcatatcaga
    24541 aaaaagggcc ggatgattcc agccctgtat ttttacttgc taaacgcagc ctggaagaca
    24601 gctaccagcg cgtcgttctg actctgagtc agagtatgac ctttcggatc gatgaactgt
    24661 aggctgctgc ggttatctaa atcgccaacc tgcagtttat agtcaccgga tgccaggcct
    24721 ggatcgctcg cgcccagttc ctgccagtcg ctgtcagaca gcggcttata agttacggcc
    24781 atgttgccct gcgaacgggt gctgtcggtc actttcatgc ccactttttc cagcgacgct
    24841 ggcagacgtt gccaaaccac attgaacggc ccgcgtacga ccagcattgg taaaccggtg
    24901 tcatcagctg cactttgtac gtccatagtg gtggaggcac gattttgcgc agcgttcgcg
    24961 gcgtcagtgg cagatttatc cagaccggcg gaaataacgt tcatcatctc cgtgctgtaa
    25021 cgctgcatgg aagccgcgtc tgcaaccggt ttgcccgcct gttccaggtt cagcagttta
    25081 accgtaaccg cctgctgata accctgcggc ttaacagaga tttgataacg accacgatac
    25141 tgctcgtctt cgtccagacg gttccattgt acccaatcgg tggtcagtgt ctgaccagca
    25201 tcatcacgtt gggtgatggt gtagtttttc gcctgcagca cgctaaccac ctgcggccac
    25261 agagtattgc cacgaccatt ttccaccagc aatgaagcgg tatcgcccgt gaactgggta
    25321 cgcgcgccag aaaccagtgc cagcggctgg gctggtggac gaatgtccag cgccttaccg
    25381 acagcaccac taccgttggt caccgggatt gcataatcac cggaggtcac cggcaaaatc
    25441 attccagccg gggcatgaag ctccgcaagc ggtgccgctt ccaggtaggc ttcatcacca
    25501 ctgacctgac gcttatagcg tgagtcagaa ctacaggcag cgagtaataa aacaagcgaa
    25561 acacccgcaa cctttgccag gcgcgacttt tgaacagagt aagccatcaa atctccctaa
    25621 actttacagc aaaccggcat gcttaagcgc cgctctgacc gtctcacgac cactgtcggt
    25681 gattggtgtc attggcaggc gcagcgtatc ggtcgccaca agacccagtt ccttacatgc
    25741 ccatttcacc gggattggat tgggttcgac aaatagtttg ttgtgtaatg gcatcagacg
    25801 ctgattaata acgcgtgcct cggcaaaatg cccttctgct gccagtttgc acatctgggc
    25861 catatcacgc gctgcgacgt tagccgtaac ggaaataacc ccatgaccgc ccaattgcat
    25921 gaagtccagc gcgctcgcat catcgccgct cagcagaaca aaatcatctg aaaccagctc
    25981 tttgatctgg tttacacgcg ttaagttccc tgttgcctct ttgattccga taatattttt
    26041 tactttcgcc agacggccca ccgtttccgg gagcagatcg cagccagtac gggacggcac
    26101 attatacaga atttgcggca ggtcagtatg ctcagcgatg gctttgaaat gctgatacaa
    26161 accttcttgc gacggacgat tgtagtaagg ggttaccgtc aggcagccga cgataccact
    26221 gtcattgaag cgctgcgtca ggctaatggc ttccgcagta gcgttagcgc cggtcccggc
    26281 aattaccgga atgcgcccat cagccagatc cagcgtcatc atcaccacat cagcatgttc
    26341 gtcatgattt aaggtagcgg actcgccagt ggtgccaaca gaaacgatcg ccgaagtacc
    26401 gctggcgaca tgataatcaa tcagtttttt caagctagcc cgacagacat tacctttttc
    26461 atccatcgga gtaacaatcg cgacaatact tcccgtgaac atgggccatc ctctgtgcaa
    26521 acaagtgtct caatggtacg tttggtatgg cattaaaagc aagcagacag aaccgttctg
    26581 attgttgtat gcatgatttt tttatgcttt ccttaagaac aactcacccc ttaaaggaat
    26641 aaccagtttg acactgtcat cgcaacatta tctggtgatc actgcgttgg gtgccgatcg
    26701 ccctggaatt gtgaacacca tcacccgtca tgtcagtagt tgcggctgta atattgaaga
    26761 cagtcgcctg gcgatgctgg gagaagagtt cacgtttatc atgctgcttt ccggttcatg
    26821 gaatgccatc actctgattg aatcaacgtt accgttgaaa ggtgccgaac tggatctttt
    26881 aatcgtgatg aagcgcacga cggcgcgtcc gcgtccgcca atgccagcat ctgtctgggt
    26941 tcaggtcgat gttgcagact ccccgcattt aattgaacgc ttcacagcac ttttcgacgc
    27001 gcatcatatg aacattgcgg agctggtgtc gcgcacacaa cctgctgaaa atgaacgggc
    27061 tgcgcagttg catattcaga taaccgccca cagccccgca tctgcggacg cagcaaatat
    27121 tgagcaagcg ttcaaagccc tatgtacaga actcaatgca caaggcagta ttaacgtcgt
    27181 caattattcc caacatgatg aacaggatgg agttaagtaa tgaatccact gaaagccggt
    27241 gatatcgcac cgaaatttag cttgccggat caagacggag aacaagttaa tttgaccgac
    27301 ttccagggac agcgtgttct ggtttatttc tacccgaaag ccatgacccc cggctgtacc
    27361 gtacaggcct gcggcttacg cgataacatg gatgagttga aaaaagcggg cgttgatgtg
    27421 ctgggtatca gcaccgataa acccgaaaaa ctctcccgtt ttgcggaaaa agagctgctt
    27481 aactttacgc tcctgtctga tgaggaccac caggtgtgcg aacaattcgg cgtctggggt
    27541 gaaaagtcct tcatgggcaa aacctacgat ggtattcatc gcatcagctt cctgattgac
    27601 gctgatggca aaatcgaaca tgtctttgac gacttcaaaa ccagcaatca ccacgacgtt
    27661 gtgctgaact ggctgaaaga acacgcctga ttactttgct ccattccgtg ctggctgcgc
    27721 ttgcggccag catacctcac ttctcgtgat caagatcaca ttctcgcttt cccctgcgac
    27781 acgggtgtcg aatccatttt ttgctgaacg ttaatgacca tcatttttgt accgttcaga
    27841 atccagttaa tacataactt attgaataga ttgagttaat cagaatggca ccctttatgc
    27901 aatatgaaat gcaatgtttc atatcatttt caaggagccg acatgaaccg ctttgtggtg
    27961 gccgaaccac tgtggtgtac aggatgtaat acctgtctcg ctgcctgttc ggacgtgcat
    28021 aaaacgcaag gtttacagca acacccgcgc ctggccctgg cgaagacgtc aacaatcact
    28081 gcccctgtcg tgtgtcatca ctgtgaggaa gccccttgcc tgcaggtctg cccggtcaat
    28141 gccatctctc agagggatga tgcgatccaa ctcaacgaaa gcctctgtat tggctgcaag
    28201 ctttgcgccg tggtctgccc atttggcgca atcagcgctt caggaagccg tccggtgaat
    28261 gcccatgcgc aatatgtttt tcaggctgaa ggctcactca aagacggcga agaaaacgtg
    28321 ccgccccaac atgcgttgct gcgctgggaa ccgggtgtcc agaccgtcgc ggtgaaatgc
    28381 gacctgtgtg atttcttgcc agaaggtccg gcctgcgttc gcgcttgccc gaatcaggcg
    28441 ttacggctga tcaccgacga tagcctgcaa cgtcagatga aagaaaaaca gcgccttgcc
    28501 gcaagctggt ttgccaatgg cggggaggat cccctttccc tcactcagga gcaacactaa
    28561 tggatgccct gcaattatta acctggtcgc tgatcctcta tctgtttgcc agtctggctt
    28621 cgctgttttt actcggtctg gacagactgg ctattaagct ttccggcatc acatcgctgg
    28681 tgggcggcgt gattggcatc atcagcggaa ttacgcaatt acatgctggt gtaactttag
    28741 tcgcccattt tgccacccct tttgactttg ccgatttaac cctgcgaatg gatagcctct
    28801 cggcatttat ggtgctggtt atctccttgc tggtggtggt ttgttcgctc tattcattga
    28861 cttatatgcg cgaatacgag ggcaaaggcg cggcggcgat gggcttcttt atgaatcttt
    28921 tcatcgcatc gatggttgcc ctgctggtga tggacaacgc tttttggttc atcgtgctgt
    28981 ttgaaatgat gtcgctgtct tcctggtttc tggtcattgc caggcaggat aaaacgtcga
    29041 tcaacgctgg catgctctac ttttttatcg cccacgccgg atcggtgctg attatgatcg
    29101 ccttcttgct gatggggcgc gaaagcggca gcctcgattt tgccagtttc gcacgctttc
    29161 actttctccg gggctggcgt cggcggtgtt tctgctggcc tttttcggtt ttggcgcgaa
    29221 agccgggatg atgccgttgc acagctggtt gccgcgcgct caccctgccg caccatcgca
    29281 cgcttcggcg ttgatgtctg gcgtaatggt caaaatcggt attttcggca tcctgaaagt
    29341 agcgatggat ctgctggcgc aaacgggttt gcctctgtgg tggggcattc tggtgatggc
    29401 gatcggcgca atctccgcgc tcctgggcgt gctatatgcg ctggcggaac aggatatcaa
    29461 acggctgctg gcctggagta ccgtcgaaaa cgtcggcatt attttgctgg cagtcggtgt
    29521 ggcgatggac ggtctgtcac tgcacgaccc gctgctcacc gtggttggac tgctcggcgc
    29581 actgtttcat ctgctcaacc atgcgctgtt caaagggctg ctatttctcg gcgcggacgc
    29641 gattatttcg cgtttgcata cccacgacat ggaaaaaatg ggggcactgg cgaaacggat
    29701 gccgtggaca gccgcagcat gcctgattgg ttgcctcgcg atatcagcca ttcctccgct
    29761 gaatggtttt atcagcgaat ggtacacctg gcagtcgctg ttctcactaa gtcgtgtgga
    29821 agccgtagcg ctacaacttg cgggtcctat tgctatggta atgctggcag tcactggtgg
    29881 gctggcagta atgtgcttcg tcaaaatgta cggtattact ttctgtggtg cgccgcgcag
    29941 tacacacgct gaagaggcac aggaagtgcc aaatacgatg atcgtcgcca tgctactgct
    30001 cgcggcactc tgcgtattaa ttgcgcttag tgccagttgg ctggcaccga agataatgca
    30061 tattgcccat gcgtttacca atacccctcc cgtcactgtc gccagcggaa tagcacttgt
    30121 acccggcacg tttcatacac gggtcactcc ttcattactg ttgctgttac tactggcgat
    30181 gcctttgctg ccaggccttt actggctgtg gtgtcgttcg cgccgcgcag cgtttcgtcg
    30241 cacaggagat gcctaggcat gcggctacgg ctgggaaaac gcgatggctc cgtcaggcaa
    30301 tggcgtgatg cagccgctgc gtgtggtctt ctctgcgtta tttcgtctac gacaacagct
    30361 cgaccctacg ctgaggctaa ataaaggtct tgcgcacgtc accgccaggg ctcagagcac
    30421 agaacccttc tgggatgagc gggtgatccg ccccatcgtg agcgccaccc aacggctggt
    30481 caaagaaata cagcatctgc aaagcggcga ctttcgtctc tattgcctgt atgtggtcgc
    30541 cgcactggtt gtgctgctaa tcgctattgc cgtctaagga aatcaccatg agacaaactc
    30601 tttgcgacgg atatctggtc atttttgcgt tagcacaggc cgtgattctg ctgatgctaa
    30661 ccccactttt tacgggtatt tcccggcaga tacgcgcgcg tatgcactcc cgccgcgggc
    30721 cggggatctg gcaggattat cgcgatatcc acaaactgtt taaacgccag gaagttgcgc
    30781 cgacatcttc aggtctgatg ttccgcctga tgccgtgggt attaatcagc agcatgctgg
    30841 tgctggcgat ggccttacca ctatttatta ccgtttcccc ttttgcgggc ggcggcgatc
    30901 tgctcaccct tatctatctt cttgccctgt ttcgtttttt ctttgctctt tccgggctgg
    30961 ataccggaag tccgtttgcg ggagtcggtg ccagtcgcga gttgacgctc ggcattctgg
    31021 tcgaaccaat gcttattctc tcactgctgg tattggcgct gatagcaggt tccacgcata
    31081 tcgagatgat cagcaatacg ctggcgatgg gctggaactc gccgctaacc accgtactgg
    31141 cgttactggc ctgtggtttt gcctgcttca ttgagatggg aaaaattccc tttgatgttg
    31201 ctgaagcaga acaggaatta caggaaggcc cgctgaccga atattccggt gccgggctgg
    31261 cgctagcgaa atgggggcag gggctgaaac aggtcgtgat ggcagcactg tttgtggccc
    31321 tgtttctgcc ctttgggcgc gcgcaagaac tttctctcgc ctgcctgctg acttcacttg
    31381 tcgttacgct gctcaaggtt ttgctgattt ttgtactggc ctcaatcgca gaaaacacgc
    31441 tggcacgcgg gcgtttttta ctcattcacc atgtgacctg gcttggcttc agccttgctg
    31501 cgcttgcatg ggtcttctgg ttaaccggtc tgtaaggagc actgacggaa tatggaaaat
    31561 cttgctctga cgacgttaat gctgcctttt atcggcgcac tggtcgtttc gttttcgcca
    31621 caacgtcggg ccgccgaatg gggggttttg ttcgccgcgc tgaccacgct gtgcatgttg
    31681 tcgctgatct ccgcgtttta tcaggccgat aaagttgccg tcacgttgac gttggtcaac
    31741 gtgggggatg tggcattgtt tggcctggtc attgatcgcg tgagtacgct gattctgttt
    31801 gtggtggtgt ttctcggttt gctggtcacg atctactcca cgggttatct gacggataaa
    31861 aatcgcgaac acccgcataa cggcacgaat cgttattacg catttttgct ggtgtttatc
    31921 ggcgcgatgg cgggactggt actctcctcg acgctgctcg gtcagttgtt gttttttgaa
    31981 attacgggcg gctgctcctg ggcgttgatc agttattacc agagcgataa agcgcagcgt
    32041 tcagcactaa aagcgttgct tatcacccat atcggttcgt tggggttgta tcttgccgcc
    32101 gccacgctgt ttttgcagac cggaacgttt gtgcttagcg cgatgagcga gttacatggc
    32161 gacgcacgtt atctggttta tggcggcatc ctgtttgccg cgtgggggaa atcggcccag
    32221 ctaccgatgc aagcgtggct accggacgca atggaagcgc caacaccgat cagcgactat
    32281 ctccacgccg catcgatggt gaaagtgggc gtttacattt ttgcccgcgc cattatcgac
    32341 ggcggcaata tcccgcatgt gattggcggc gttggcatgg tcatggcact ggtcaccatt
    32401 ctttatggct ttctgatgta tttgccacag caggatatga agcggttgct ggcctggtcg
    32461 accatcactc aacttggctg gatgttcttc ggcttgtcgc tctccatctt cggctcgcgg
    32521 ctggcgctgg agggtagcat cgcctacatc gtcaaccacg cgttcgctaa aagcctgttt
    32581 ttccttgtag caggtgcgct gagttacagc tgcggcacgc gcttgttgcc gcgtctgcgt
    32641 ggcgtattgc acaccctgcc gttgccaggc gtgggtttct gcgtggcagc gctggcaatt
    32701 accggtgtgc cgccgttcaa cggcttcttc agtaaattcc cgctgtttgc cgccggtttt
    32761 gcgttgtcag tggagtactg gatcctgctg cccgccatga ttctgctgat gattgaatcg
    32821 gtcgccagtt tcgcctggtt tattcgctgg tttggtcgcg tcgtgccggg caaaccgagc
    32881 gaggccgtcg ccgatgccgc accgctgcca gggtcaatgc gcctggtgtt gattgtactg
    32941 attgtgatgt cgctgatttc cagcgtaatc gccgcgacct ggttgcagta aggagatgat
    33001 gaatgaccgg ttctatgatc gtaaataatc tggcgggact gatgatgctg acatcgctgt
    33061 ttgtgattag cgtcaaaagc tatcgcctgt catgcggatt ttaggcctgc cagtcgctgg
    33121 tgctggtgtc tattttcgcc actctctcgt gcctgttcgc cgcagagcaa ctgctgatct
    33181 ggtccgccag cgcctttatc accaaagtgc tgctggtacc gttaatcatg acttacgctg
    33241 cacgaaatat tccccagaac atcccggaaa aagcgttatt cggtccggca atgatggcac
    33301 tgctcgcggc gttaattgtc ctgctttgcg catttgtcgt tcagcccgtg aagctaccga
    33361 tggctaccgg gctgaaaccg gcgctggcgg tagcgttagg tcattttctg cttggcctgc
    33421 tgtgcattgt cagccagcgc aatatcctgc ggcaaatttt tggttactgc ctgatggaaa
    33481 acggctccca tctggtgctg gcgcttcttg cctggcgagc accggaactg gtggaaatag
    33541 gtatcgctac cgacgccatc ttcgccgtca ttgtgatggt gttactggca agaaaaatat
    33601 ggcgtaccca cggcacgctg gacgtgaaca acttgaccgc gctgaaggga taatgagatg
    33661 agttattctg tgatgttcgc tttactcctg ctcacgccgc tgcttttttc gctgctctgt
    33721 tttgcctgcc ggaaacgggg gctctctgcg acttgtacgg tgactgtatt acatagctta
    33781 gggatcacac tgctgctgat tctggcactc tgggtggtcc aaactgccgc tgatgcagga
    33841 gaaatattcg ctgcgggact gtggcttcat attgatggtc tgggcggttt gttcctcgcc
    33901 attcttggtg tgattggctt tctcaccggt gtttactcga ttggctacat gcgtcatgaa
    33961 gtggcacacg gcgagctttc acccgttaca ctgtgcgatt actacggttt cttccatctg
    34021 tttttgttca ccatgctgct ggttgttacc agcaataacc tgattgtgat gtgggcggcg
    34081 atcgaagcca ccaccttaag ctcggcgttt ctggtaggca tttacggtca gcgttcatcg
    34141 ctggaatcag catggaagta catcattatt tgtactgttg gtgtcgcttt tggtctgttc
    34201 ggtaccgtgc tggtatacgc caacgccgcc agcgttatgc cgcaggcaga aatggcgata
    34261 ttctggagcg aggttcttaa gcaatcgtcc ttgcttgacc caacattaat gctgttggcc
    34321 tttgtgtttg tgctaattgg ctttggtacc aaaaccgggc tatttcccat gcacgcctgg
    34381 ctgccggatg ctcacagtga agcgccgagt ccggtcagcg ccctgctctc cgccgtattg
    34441 ctgaactgcg cgctgttggt gctgattcgc tattacatca ttatttgcca ggccatcggc
    34501 agcgatttcc ccaaccggtt gttgctcatc ttcggcatgt tgtcggttgc cgtggcggca
    34561 tttttcattc tggtacagcg ggacattaag cgtctgctgg cgtactccag cgtggagaac
    34621 atggggctgg tcgcggtggc gctaggcatt ggcgggccgc tgggaatttt tgccgcgctg
    34681 ctgcacacct taaaccacag tctggcaaaa acgctgctgt tctgcggttc cggcaatgta
    34741 ctgctcaagt acggcacgcg cgatctcaac gtcgtctgtg ggatgctcaa aatcatgcca
    34801 tttaccgccg tgctgtttgg cggcggtgcg ctggcgctgg cagggatgcc gcccttcaac
    34861 atttttctta gcgaatttat gaccgttacc gcaggactgg cacgtaatca cctgctgatt
    34921 atcgtcctgc tgttattgct gttaacgctg gtgctcgcgg gcctggtacg gatggctgcg
    34981 cgggtgttaa tggcgaaacc gccgcaggcc gttaaccggg gtgatctcgg ctggttgacc
    35041 acctcgccaa tggtgattct gctagtcatg atgctggcga tgggaacgca tattccacaa
    35101 cctgtcatca ggatcctggc gggcgcttcc actatagtcc tctcagggac gcacgacctg
    35161 cctgcacaac gtagcacctg gcatgatttt ttgccttcag gcaccgcatc tgtttcggag
    35221 aaacacagtg aacgttaatt catcgtcaaa tcgtggcgaa gcgattctcg ccgccctgaa
    35281 aacgcagttc cccggcgcgg tgctggatga agagcgacaa acgcctgaac aggtcaccat
    35341 tacggtgaaa atcaatctgc tgcctgacgt tgtacattat ctttattatc aacatgatgg
    35401 ctggcttcca gtcctgtttg gcaacgacga gcggacactt aacggtcatt acgcggttta
    35461 ttatgccctt tcaatggaag gggccgaaaa atgctggatc gtggtgaagg cactggtcga
    35521 tgccgacagt cgggagtttc cgtcagtcac accgcgcgtc cctgccgcgg tctggggcga
    35581 gcgagaaatt cgcgatatgt acgggctgat tccggttggc ctgccggatc agcgtcgcct
    35641 ggtgttgccc gatgactggc cggaagatat gcatccgctg cgcaaagatg cgatggatta
    35701 tcgactgcgc cctgaaccga cgacagattc cgaaacgtat gcgtttatca atgagggcaa
    35761 cagcgatgcg cgggtgatcc ctgtcggccc gctgcatatc acctcctatg aaccgggtca
    35821 cttccgcttg tttgtggatg gcgagcaaat tgtcgatgct gattaccgcc tgttttatgt
    35881 ccatcgcggc atggagaaac tggcagaaac gcggatgggc tacaacgaag tgaccttctt
    35941 atcggaccgc gtgtgtggga tttgcggttt tgcccacagt gtggcctata ccaactcggt
    36001 tgaaaatgca ctggggattg aggtgccgca acgagcacat actattcgct cgattctgct
    36061 ggaagtcgag cggctacaca gtcatttgct caaccttggc ctctcctgcc attttgttgg
    36121 ttttgatacc ggctttatgc aatttttccg cgtgcgggaa aagtcgatga cgatggcgga
    36181 attgctgacc gggtcgcgta aaacctacgg tctgaatctg attggtggtg ttcgccgcga
    36241 tattctcaaa gagcaacgtc tgcaaacgct gaaactggtg cgcgagatgc gcgccgacgt
    36301 gtcggagctg gtagagatgc tgcttgctac gccgaatatg gaacaacgca ctcagggcat
    36361 tggcattctc gaccgacaaa tcgcccgtga ttatagccct gtagggccgc tgatccgcgc
    36421 agtggttttg cccgtgattt gcgctttgat cacccctacg ccgactacgg caatattcca
    36481 aaaacactgt ttacctttac cggcggcgat gttttctccc gcgtgatggt ccgtgtcaaa
    36541 gagacgtttg attcgctggc aatgctggaa tttgccctcg acaacatgcc ggatacccca
    36601 ctgctgaccg aaggctttag ctataaacct cacgcattcg cgctgggctt tgttgaagcg
    36661 ccacgcggtg aagacgtgca ctggagcatg ctcggtgata accaaaaatt gttccgctgg
    36721 cgctgtcgtg ccgccaccta cgccaactgg ccggtgttgc gttacatgct gcgcggcaat
    36781 accgtttctg acgcaccgct gattatcggt agccttgatc cctgctactc ctgtaccgac
    36841 cgtgtgacgc tggtagatgt gcgcaagcgc cagtcaaaaa ccgtgctgta taaagagatc
    36901 gaacgctacg gcattgatcg taaccgttcg ccgctgaagt aaggacagaa gatgctgaag
    36961 ttactgaaaa ctattatgcg cgccggaacc ccgacggtga aatatccctt cgcgccactg
    37021 gaggtcagcc caggctttcg cgggaaaccg gacctgatgc ccagccaatg tattgcctgc
    37081 ggtgcctgcg cctgtgcttg tccggcaaat gcgctgacta tccagaccga cgaccagcaa
    37141 aattcgcgca cctggcagct ctatctgggg cgttgtattt actgcggacg ttgtgaagaa
    37201 gtgtgcccga ccagagccat ccagcttacc aataactttg aactgaccgt cacccataaa
    37261 gccgatctct atacccacgc gacgttccat ctacaacgtt gcagccgttg cgaacgcccg
    37321 tttgccccgc aaaaaaccgt cgcactggct gctgaattgt tggcacagca acaaaatgct
    37381 ccacaaaacc gcgaaatgtt atgggcgcaa gcgagcgtct gcccggagtg caaacaacgc
    37441 gcgacgctga tcaacgacga tacagatgta ccgctggtgg ctaaggagca gctatgagtc
    37501 cagtgcttac gcaatatgtc agccagccca tcacgctgga cgagcaaacg caaaagatga
    37561 agcggcattt gctacaggat atccgtcgct cggcttacgt ttatcgcatc gattgcggcg
    37621 gctgcaacgc ctgtgaaatc gaaatttttg ctgccatcac ccccgtattc gacgcagaac
    37681 gttttggcat taaggttgtt tcatcaccgc gtcacgccga tattttgtta tttactggcg
    37741 cagtcacccg ggcgatgcgt atgcctgcac ttcgggcgta tgagtctgcc cccgatcata
    37801 aaatctgtgt ttcctacggc gcgtgcggtg tcggcggcgg tattttccgc gatctctaca
    37861 gcgtctgggg cggcagcgac accattgtcc ccattgatgt ttggatcccc ggctgcccgc
    37921 caacaccggc cgccaccatt cacggtttcg ccgtggcgct cggtttgctg caacagaaga
    37981 ttcacgcggt ggattatcgc gatcccaccg gggtgactat gcaaccgttg tggccgcaga
    38041 tcccgccatc acagcgtatc gccattgagc gagaagcgcg gcggctggcg ggctatcgtc
    38101 aggggcgaga aatttgcgat cggctcctgc gccatttaag cgacgatcct acaggaaatc
    38161 gggttaacac ctggttgcgc gatgccgacg atccacgtct caatagtatc gttcagcaac
    38221 tctttcgcgt actccggggg ttacatgact gaagagtgcg gggaaattgt tttctggacg
    38281 ctgcgaaaaa agtttgtcgc cagtagcgac gagatgccgg aacacagctc tcaggtaatg
    38341 tattactcgc tggctatcgg tcatcacgtt ggcgtgattg attgtctgaa tgtcgccttc
    38401 cgctgcccac tgacggaata cgaagattgg cttgcactgg tcgaagagga gcaagcccga
    38461 cgtaagatgc tgggggtgat gacttttggt gagattgtta ttgacgccag ccacaccgcc
    38521 ctgttgaccc gggcattcgc gccactggca gatgacgcga cgtctgtgtg gcaagcgcgt
    38581 agcattcaat tcattcatct gttggatgaa attgtgctgg aaccggctat ctatctgatg
    38641 gccagaaaaa ttgcgtgaga aggatttctc attaataagg actgttgatg gctatgtcag
    38701 acgaagcgat gtttgccccg ccgcaaggaa taacaattga agcggtaaac ggaatgctcg
    38761 cggagcggtt agcgcagaaa cacggcaagg cgtctttatt acgcgccttc atcccgctgc
    38821 cgccgccgtt cagcccggta caacttattg aactgcatgt tctcaaaagc aacttctatt
    38881 accgctacca tgatgatggc agcgatgtga cggcaacaac agagtatcag ggcgagatgg
    38941 tcgattattc gcgtcacgcc gtccttctcg gcagtagttg aatggcggag ctacgcttta
    39001 ttcgtaccca cggcagccgt tttactcccc aggattgcac actgtttaac tggctggcac
    39061 gcattatcac cccggttctg caatcatggc tcaatgatga agaacagcag gtggcgctgc
    39121 gtttgctgga gaaagatcgc gatcatcatc gggtactggt ggatatcact aatgcagtgc
    39181 tgtcacatct tgatctcgac gatctgatcg ctgacgtcgc tcgtgagatc catcattttt
    39241 tcggtctggc ttcagtcagt atggtactgg gcgatcatcg aaagaacgag aagttcagcc
    39301 tgtggtgcag cgatctttct gcctcacatt gtgcgtgtct gccacacaat atgcctggcg
    39361 acagtgtatt gctgacacaa acgctacaaa cccgacaacc gaccttgacg caccgtgcag
    39421 atgatctgtt tctctggcaa cgcgacccgt tattactctt acttgcatct aacggctgcg
    39481 aatctgcgct ccttataccg cttacctttg gcaaccatac accgggtgca ttgttgctgg
    39541 cgcatacctc ttccactctc tttagtgagg aaaactgcca gctactacaa cacatagccg
    39601 atcgcatcgc tattgccgtt ggcaatgccg atgcctgccg tagaatgacc gatttgcagg
    39661 aaagtttgca gcaagaaaac caccagctta gcgagcagct cctttcgaat ctgggcgtcg
    39721 gtgacattat ctatcaaagc caggcaatgg aagacctgct ccagcaggta gatattgtgg
    39781 cgaagagcga cagtacggtg ttgatttgcg gtgaaaccgg aaccggcaaa gaggtgatcg
    39841 ccagagcgat ccatcaactt agcccgcgac acgacaagcc gctggtcaaa atcaactgcg
    39901 ctgccatccc cgccagtctt ctggaaagtg agttattcgg tcatgacaaa ggggcgttta
    39961 ctggtgcgat taatacccat cgtggtcgtt ttgaaattgc cgatggcggc acgttgtttc
    40021 tcgatgaaat tggcgatctg ccgttagaac ttcagcctaa actgctgcgc gtattgcagg
    40081 aacgggagat tgagcgtctc ggcgggagta gaacgatccc ggtaaatgtc agagtcattg
    40141 ccgccaccaa ccgtgatttg tggcaaatgg ttgaagatcg ccagtttcgc agcgatctct
    40201 tttatcgcct gaatgtcttc ccactggaat tgcctccgct gcgcgaccgt ccggaagata
    40261 tccctctttt agcaaagcat ttcacgcaaa aaatggcgcg ccatatgaat cgttcaattg
    40321 acgccatccc gaccgaggcg ctacgccagt tgatgtcgtg ggactggccg ggcaacgtgc
    40381 gcgagctgga aaacgtgatt gagcgagcgg tactgctgac tcgcggtaac agtctgaatt
    40441 tacatcttaa tgtccgacaa agccgtttac tgccgacgct aaatgaagat tcagcgcttc
    40501 gcagttcaat ggcgcagttg ctgcacccga cgacgccaga gaatgacgaa gaagaacgtc
    40561 agcgcattgt tcaggtattg cgagaaacca atggcattgt tgccgggccc cgtggcgcgg
    40621 cgacacgatt agggatgaag cgcaccacgc tgctgtcacg aatgcagcgt ctgggaatct
    40681 cggttcgcga ggtgttgtaa tctgcttttg caggagtatg catgagaaac aaactctctt
    40741 tcgacttgca gttgagcgcc agaaaagcgg caatcgctga acggattgcc gcccataaaa
    40801 ttgcccgcag taaagtgtcg gtctttttaa tggcgatgtc cgctggcgtg tttatggcga
    40861 tcggatttac tttttacctt tccgttatcg ccgatgcccc gtcttcatag gcattaaccc
    40921 atctggtggg cggcctttgc tttacacttg gctttatttt gctggcggtt tgcggcacca
    40981 gcctgttcac ctcgtcggta atgacggtaa tggcaaaaag tcggggcgtt attagttggc
    41041 gaacttggct gattaacgca cttctggtgg cctgcggtaa tctggcaggt attgcctgtt
    41101 tcagtttgtt aatctggttt tccgggctgg tgatgagtga aaacgcgatg tggggagtcg
    41161 cgattttaca ctgcgccgag ggcaaaatgc atcatacatt tactgaatct gtcagcctcg
    41221 gcattatgtg caatctgatg gtttgcctgg cgctgtggat gagttattgc ggacgttcgt
    41281 tatgcgacaa aatcgtcgcc atgattttgt ccatcaccct gtttgtcgcc agtggctttg
    41341 agcactgtat cgccaatttg tttgtgattc cgttcgccat tgccattcgc catttcgccc
    41401 ctacctcctt ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtca
    41461 gccattttat taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag
    41521 tgctggtgag tatttgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc
    41581 cggcgaaaca gttcgtcggg ctgaagatta ttcttgcgcg atttgcccat cgggccaggc
    41641 gtggatcacg gctttgatca gcgtcgccaa tggaatggcg aagaatacgc cccagaatcc
    41701 ccacaaacca ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc
    41761 ttcggagaac aacaccggta ctagcaggtt gccgtccagc gcctgaataa tcagatacac
    41821 cgcgaagcag ctccagaatt cagtacctgc gccaaactgg aatagcgcca cgccaaccac
    41881 cggaatggtc accacaaatg cgccaatgta cggaataaga accgagaagc cgaccagcac
    41941 tgccagcagc agcgaatagt tcagcccgaa gagcaagaac cccagccagg tggcgatccc
    42001 caccacgatc atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc
    42061 cttccacacc tgtcctgcca gcccacggtt acgcggcagc acccggcgaa cggcgttcag
    42121 catctgctct ttgtctttca gcaggaagaa gaccatcaat ggcaccagca ccagatagac
    42181 ggctatggtc agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat
    42241 ggtcagcatc cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa
    42301 caccggatag cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg
    42361 catatcgcgg attaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat
    42421 cagtagcaat ataccgacaa aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca
    42481 gccaatagat tgcaggcgca cggttggcca ttccagcaaa taagccagca caatagccac
    42541 cagcaacgga gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat
    42601 aaccagcaag gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat
    42661 ttcgagcata aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg
    42721 aaatgtcaca aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg
    42781 agtgggctaa tcttcgccgt tacactcaaa ggcggcgagg tgggaacgat atttcacagt
    42841 atcggtcaaa tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa
    42901 aaaacctggt tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg
    42961 ccgacagcgc agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg
    43021 gtcaggaaat gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa
    43081 ttaatgaccc gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg
    43141 ccaattcggt taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct
    43201 ttgctttctt tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg
    43261 aaagtcaact ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg
    43321 cgcgagcgat ggaagatcaa caacgcagcg cgccgctgac ctgggtcggc gcgttaggtt
    43381 ctattttact ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg
    43441 cgggaacgcg ccaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca
    43501 ttggtattca ggtgctgcaa cgctcgggat tcgatccaca ggcgatgccg accttcctgg
    43561 aaaaattgct cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc
    43621 cgttgccgga aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg
    43681 tggtgcagtc gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt
    43741 ccggacgtaa ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcttc
    43801 agcaacgagc ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg
    43861 aggcgcgtaa aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg
    43921 atctggctac cgatatcgat cttgagcaaa acaaagccaa tgaggcaatc aatcgcctga
    43981 aaaatgcccg cgatttacgc accaatccgg tgttgcagct caacctggcg aacgcttatc
    44041 tgcaaggcgg tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata
    44101 aagatgacag caacggctgg gatttgctgg cacaggcgga agccgcgcta aataaccgcg
    44161 atcaggagct ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca
    44221 tttcgctgtt gagtagcgcc agttcgcagg tgaaattagg cagcctgcaa caagcgcgtt
    44281 acgatgcgcg catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga
    44341 tgtaattcgc attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg
    44401 ctgttcaaag agccgggaaa cgctgaacct gctgaaagaa aacggcgtgg agccagaagt
    44461 ggtgctctac cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct
    44521 ggggatgaat agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactaaa
    44581 cctggctgac agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa
    44641 gctgatggag cgcccgatta tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga
    44701 gcaggtactg gaaatcgtgg gttaagatgt gctccatcgc tagttgaagc acattgccgg
    44761 atgcgacgct tgccacgtct tatccggcct acgaatacta tggattaact tcgcgtaggt
    44821 caggcgttcg cgccgcatcc gacaataaac accttatcta caacttcaga atttctttca
    44881 caaacggaat ggtcagctta cgttgcgcgg taatcgacgc acgatccaac tgatccaacg
    44941 tcataaatag cgtgcgcatt tctctgtcga gccgcttcag caagaaacgc cccacatctt
    45001 ccggcagttc aaaaccacgc aaacgcgcgc gtaactgtag cgcctgcaac ttatcttcat
    45061 cagaaagtgg ctgcaatttg tagatctgcc cccagtcgag acgcgacgcg agatccggta
    45121 atcccagatt caactgccgc ggtggacgat cgccggtgat caacaaccgt gttttgcccg
    45181 attccagaat tcgattgtag agatcgaaaa tcgccatctc ccacaactca tcgcctgcaa
    45241 tacactcaat gttgtcgata cagaccagcg acaaatgctc cataccgtcg agcacttccg
    45301 gaacaaacca ggtgcgttta tccagcggga catagcccac cgcatcgcca cgctgcgaca
    45361 attccgcgca agccgcgtgc agcagatggc tgcgccccgc gccttcgcgt gcccagagat
    45421 agatgtaacc gctatgttcc tgacgcagca cgttttgcag cgcggccagt aaagaggagt
    45481 tatcccccgg ccagaaactt gcaaaggttt cgtcgtcagg aagataaagt ggcaaagaga
    45541 gctgtgccgg tgtgttcaga gatacctcaa ccaggatttc acaaaatcgc gagaagttta
    45601 ccacagaatc ccatgatgtt tgaaccgggc agcaacactg cccggtcggt acattatttg
    45661 tctgttatat ccgcgtcttc tgcgtccagc accacttctt cagggcgcag cacactgatc
    45721 agtttgaaaa tcaaacttaa gccgataccg acgatggtcg ccagcgccat accttttaac
    45781 tccgccgcgc cgatgtttac cttcgcgcca ctgacgccga tgatcaaaat cacggaagtc
    45841 aggatcaggt tctgtgcttt gttgtaatcc actttcgatt cgatcaaaac acgaataccg
    45901 gaagcaccaa tgacaccata aagcagcagc gaaacgccgc ccatcaccgg caatgggatc
    45961 atctggatag cggcagccag tttaccgacg caggaaagca ggatagcgaa aatcgccgcc
    46021 ccgccgataa cccaggtact gtaaacacgg gtgatcgcca tcacgccaat gttttctccg
    46081 taagtggtat ttggcgtaga gccaaagaag ccggagatca cggtcgacag gccgttagca
    46141 aacatcgaac ggtgcagacc tggatcgcgc agcagatctt ttttgacgat attagccgtt
    46201 actaccaggt gccctacgtg ttcggcaata accactaacg ccgctggcag aatagtcaga
    46261 atggcaaacc actcgaagcg cggcgtatag agggttggca gtgcaaacca gtgagcatta
    46321 ataatcggcg tggtatcgac aattcccatt gcgaaagaga gcgcgtaccc caccagcacg
    46381 ccaattaaaa tcgggataat tgccaggaaa ccacgaaaca gcacggaacc taaaaccgtg
    46441 accgccaggg tggtaataga gatgatgatg gttttggagt ttggcgtttg cccttcagcc
    46501 gggagtaaac ccgccatacc ggcagctacg cccgccagct ccagaccgat gacggcaacg
    46561 attgcgccca ttgccgcagg tggaaacagc acgtccaacc agccggtccc cgctttcttc
    46621 acgataaaag aaaccaggca gaacagcacg ccgcacataa taaagccgcc cagcgcgact
    46681 tcatacccta acggcaacag taacaatacc ggtgaaataa aggcaaagct ggaaccaaga
    46741 taagccggaa ttttcccttt acagatgaag agatacagca gcgttccaat accgttaaat
    46801 aacagtacag tcgccgggtt aatatgaaat aagacgggca ccaggacggt tgcaccaaac
    46861 atggcgaaca aatgttgcaa actaagcggg attgtctgta aaagtggcgg tctttcactc
    46921 accccgatag cacggcgcgt catagtatta tcctctgtat tatgtgttat aggcgcttta
    46981 ctcaaaaaaa agccgactct taaagtcggc tttaattatt tttattcttt atttcgtacc
    47041 aaagattttg tcaccggcat cgccgaggcc cggaataatg tatccgtgct cgttcagtcc
    47101 ctggtcaatc gatgcagtat acagttcgac gtccgggtgc gctttttcca gcgcagcgat
    47161 accttctggc gcagcgacca gcaccagaac tttgatgctg ctgcagcccg cttttttcag
    47221 caggtcgatg gtcgcgataa cggaaccacc ggttgccagc attgggtcaa cgatcagcgc
    47281 catacgctca tcgatgttag aaaccagttt ctggaagtac ggtaccggct ccagcgtttc
    47341 ttcattacgg tacataccga caacgctgat gcgcgcgctc ggaacgtttt ccagcacacc
    47401 gtccatcata ccaagacccg cacgcagaat tggcacaacg gtaattttct tacctttgat
    47461 ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt actttttccg tttcgaggtc
    47521 ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa gcgagttcgc gaaagcgctt
    47581 ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt ttgacgagtg ggtgtttgac
    47641 ttccacgatc ttcatactct ttctcctttg aggggcagcc acaaaaaaaa tcgacggatt
    47701 atacctcctt tcttcaaggc ggcaatatta ttttcgttga ctttagtcaa aatgataaca
    47761 gtttgagata aagttatttt atattcaaat ggttatgaaa gaagattatt ccatccgaaa
    47821 actaaccttt gccctggcac aagtcttctt tcgccgcgcg cccggggaaa agacgtgcaa
    47881 aaaggttgtg taaaacagtc tcgcaaacgt ttgctttccc tgttagaatt gcgccgaatt
    47941 ttatttttct accgcaagta acgcgtgggg acccaagcag tgaccgataa aacctctctt
    48001 agctacaaag atgccggtgt tgatattgac gcgggtaatg ctctggttgg aagaatcaaa
    48061 ggcgtagtga agaaaacgcg tcgtccggaa gtgatgggcg gtctgggcgg cttcggtgcg
    48121 ctgtgtgcat tgccgcaaaa atatcgtgaa cccgtgctgg tttccggcac tgacggcgta
    48181 ggtaccaagc tgcgtctggc aatggactta aaacgtcacg acaccattgg tattgatctg
    48241 gtcgccatgt gcgttaatga cctggtggtg caaggtgcgg aaccgctgtt tttcctcgac
    48301 tattacgcaa ccggaaaact ggatgttgat accgcttcag cagtgatcag cggcatcgcg
    48361 gaaggttgtc tgcaatcggg ctgttctctg gtgggtggcg aaacggcaga aatgccgggg
    48421 atgtatcacg gtgaagatta cgatgtcgcg ggtttctgcg tgggcgtggt agaaaaatca
    48481 gaaatcatcg acggctctaa agtcagcgac ggcgatgtgc tgattgcact cggttccagc
    48541 ggtccgcact cgaacggcta ttcgctggtg cgcaaaattc ttgaagtcag cggttgtgat
    48601 ccgcaaacca ccgaacttga tggtaagcca ttagccgatc atctgctggc accgacccgc
    48661 atttacgtga agtcagtgct ggagttgatt gaaaaggtcg atgtgcatgc cattgcgcac
    48721 ctgaccggcg gcggcttctg ggaaaacatt ccgcgcgtat tgccagataa tacccaggca
    48781 gtgattgatg aatcttcctg gcagtggccg gaagtgttca actggctgca aacggcaggt
    48841 aacgttgagc gccatgaaat gtatcgcacc ttcaactgcg gcgtcgggat gattatcgcc
    48901 ctacctgctc cggaagtgga caaagccctc gccctgctaa atgccaacgg tgaaaacgcg
    48961 tggaaaatcg gtatcatcaa agcctctgat tccgaacaac gcgtggttat cgaataatga
    49021 atattgtggt gcttatttcc ggcaacggaa gtaatttaca ggcaattatt gacgcctgta
    49081 aaaccaacaa aattaaaggc accgtacggg cagttttcag caataaggcc gacgcgttcg
    49141 gccttgaacg cgcccgccag gcgggtattg caacgcatac gctcatcgcc agcgcgtttg
    49201 acagtcgtga agcctatgac cgggagttga ttcatgaaat cgacatgtac gcacccgatg
    49261 tggtcgtgct ggctggtttt atgcgcattc tcagcccggc gtttgtctcc cactatgccg
    49321 ggcgtttgct gaacattcac ccttctctgc tgccgaaata tcccggatta cacacccatc
    49381 gtcaggcgct ggaaaatggc gatgaagagc acggtacatc ggtgcatttc gtcaccgatg
    49441 aactggacgg tggcccggtt attttacagg cgaaagtccc ggtatttgct ggtgatacgg
    49501 aagatgacat caccgcccgc gtgcaaaccc aggaacacgc tatttatcca ctggtgatta
    49561 gctggtttgc cgatggtcgt ctgaaaatgc acgaaaacgc cgcgtggctg gatggtcaac
    49621 gtctgccgcc gcagagctac gctgccgacg agtaatgccc ccgtagttaa agcgccagct
    49681 ctgccgctgg cgtttttcaa ttcacctgta aatcgcaagc tccagcagtt tttttccccc
    49741 ttttctggca tagttggaca tctgccaata ttgctcgcca taatatccag gcagtgtccc
    49801 gtgaataaaa cggagtaaaa gtggtaatgg gtcaggaaaa gctatacatc gaaaaagagc
    49861 tcagttggtt atcgttcaat gaacgcgtgc ttcaggaagc ggcggacaaa tctaacccgc
    49921 tgattgaaag gatgcgtttc ctggggatct attccaataa ccttgatgag ttctataaag
    49981 tccgcttcgc tgaactgaag cgacgcatca ttattagcga agaacaaggc tccaacgctc
    50041 attcccgcca tttactgggc aaaattcagt cccgggtgct gaaagccgat caggaattcg
    50101 acggcctcta caacgagcta ttgctggaga tggcgcgcaa ccagatcttc ctgattaatg
    50161 aacgccagct ctccgtcaat caacaaaact ggctgcgtca ttattttaag cagtatctgc
    50221 gtcagcacat tacgccgatt ttaatcaatc ctgacactga cttagtgcag ttcctgaaag
    50281 atgattacac ctatctggcg gtggaaatta tccgtagcga tagcatccgt tacgcgctgc
    50341 tggagatccc atcagataaa gtgccgcgct ttgtgaattt accgccagaa gcgccgcgtc
    50401 gacgcaagcc gatgattctt ctggataaca ttctgcgtta ctgccttgat gatattttca
    50461 aaggcttctt tgattatgac gcgctgaatg cctattcaat gaagatgacc cgcgatgccg
    50521 aatacgattt agtgcatgag atggaagcca gcctgatgga gttgatgtct tccagtctca
    50581 agcagcgttt aactgctgag ccggtgcgtt ttgtttatca gcgcgatatg cccaatgcgc
    50641 tggttgaagt gttacgcgaa aaactgacta tttcccgcta cgactccatc gtccccggcg
    50701 gtcgttatca taattttaaa gactttatta attttcccaa tgtcggcaaa gccaatctgg
    50761 tgaacaaacc actgccgcgt ttacgccata tttggtttga taaagcccag ttccgcaatg
    50821 gttttgatgc cattcgcgaa cgcgatgtgt tgctctatta tccttatcac acctttgggc
    50881 atgtgctgga actgctgcgt caggcttcgt tcgacccgag cgtactggcg attaaaatta
    50941 acatttaccg cgtggcgaaa gattcacgca tcatcgactc gatgatccac gccgcacata
    51001 acggtaagaa agtcactgtg gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca
    51061 ttcactgggc gaagcgcctg accgaagcag gcgtgcacgt tatcttctct gcgccggggc
    51121 tgaaaattca cgccaaactg ttcctgattt cacgtaaaga aaacggtgaa gtggtccgtt
    51181 acgcacacat cgggaccggg aactttaacg aaaaaaccgc gcgtctttat actgactatt
    51241 cgttgctgac cgcagatgcg cgtatcacca acgaagtacg gcgggtattt aactttattg
    51301 aaaacccata ccgcccggtg acatttgatt atttaatggt gtcaccgcaa aactctcgcc
    51361 gtctgttata tgaaatggta gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagtg
    51421 gtatcaccct gaagctaaat aaccttgtcg ataaaggcct ggttgatcgt ctgtatgcgg
    51481 cctccagctc cggcgtaccg gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca
    51541 atctggaagg cattagcgac aacattcgtg ccatcagtat tgttgaccgt taccttgaac
    51601 atgaccgggt ttatattttt gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg
    51661 actggatgac gcgcaatatt gattatcgta ttgaagtggc gacaccgctg ctcgatccgc
    51721 gcctgaagca gcgggtgctg gacatcatcg acatattgtt cagcgatacg gtcaaagcac
    51781 gttatatcga taaagaactc agtaatcgct acgttccccg cggcaatcgc cgcaaagtac
    51841 gggcgcagtt ggcgatttac gactacatca aatcactcga acaacctgaa taaccctatg
    51901 ccaatacacg ataaatcccc tcgtccgcag gagtttgctg cggtcgatct tggttcaaac
    51961 agttttcaca tggtcatagc ccgtgtggta gatggtgcca tgcagattat tggccgcctg
    52021 aaacagcggg tgcatctggc ggacggcctg gggccagata atatgttgag tgaagaggca
    52081 atgacgcgcg gtttgaactg tctgtcgctg tttgccgaac ggctacaagg gttttctcct
    52141 gccagcgtct gtatagtcgg cacccatacg ctgcgtcagg cgctgaacgc cactgacttt
    52201 ctgaaacgcg cggaaaaggt cattccctac ccgattgaaa ttatttccgg taatgaagaa
    52261 gcccgtctga tttttatggg cgtggaacat acccaaccgg aaaaaggtcg caaactggtt
    52321 attgatattg gcggcggatc tacggaactg gtgattggtg aaaatttcga acctattctc
    52381 gttgaaagcc gccggatggg ttgtgtcagc tttgcccagc tttattttcc tggcggggtc
    52441 atcaataaag agaattttca gcgcgctcgc atggcggcag cacaaaaact ggaaacttta
    52501 acctggcaat tccgtattca gggctggaac gttgcaatgg gcgcttccgg taccataaaa
    52561 gccgcccatg aagtgttaat ggaaatgggc gagaaagacg ggataattac cccggaacgt
    52621 ctggaaaaac tggtaaaaga aattttacgt caccgtaatt tcgcatcgct gagtttaccg
    52681 ggtctttccg aagagcggaa aacagtcttc gttcctggac tggcgatttt atgtggtgtg
    52741 tttgatgctt tagccatccg tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta
    52801 ctgtatgaaa tggaaggacg tttccgtcat caggatgtgc gtagtcgcac cgccagcagc
    52861 ctcgccaacc agtatcacat agacagcgaa caggcccgac gagtgctgga taccactatg
    52921 caaatgtacg aacagtggcg ggaacagcaa ccgaagctgg cgcatccgca actggaggcg
    52981 ctactgcgat gggccgccat gctgcatgag gtcgggttga atatcaacca cagcggtttg
    53041 catcgccact ccgcttatat tctgcaaaac agtgacttgc cgggttttaa tcaggaacag
    53101 cagctgatga tggcgacact gctgcgctat caccgtaaag cgattaagct cgacgatctg
    53161 ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac tgatacagct attgcgcctt
    53221 ggcgtattac taaacaatca acgtcaggca accaccacac cgccaacatt gacactgatt
    53281 actgatgaca gtcactggac actgcgtttc ccgcatgact ggtttagtca gaatgcgctg
    53341 gtactgcttg atctggaaaa ggagcaagaa tactgggaag gcgtggctgg ctggcggttg
    53401 aaaattgaag aagaaagtac cccagaaatc gccgcttaat gctttgcggg ccgacgagaa
    53461 atgtcggccc gcattattca ggcactttcg cgaatgggtt cgatttcatt cagcgtatca
    53521 attaacggtt gcggcttacc aataagataa ccctgcatat aatcgatccc caaagagagc
    53581 accgcctcgc ggatctcttc gttttcaacg tactctgcca ctacccgcat tttcttcatt
    53641 cgcgccagat ggcaaatcga cgccacaatc tgataatcca gactatttga cacaatattg
    53701 cggataaagc tgccgtcaat tttaagcaga tcggcattca catttttaag ccgtgcatag
    53761 ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc agcctaattc ctgaagatgc
    53821 tgcaaggtga tttgcgcctg cttaacattg gtcagagcat tactttcggt gacttcaaaa
    53881 ataagttgcc acgcttcaat ctgatattta gccagcaact gactgacttc aacaggaaaa
    53941 cgagcctgac ataccgaggt tggagacaga ttaatagcaa aacggtgagc gggcatcttc
    54001 gctctgtttt cagccataaa ttgcagcgta tgctcaatga cccacatgtc gatactcgac
    54061 gataaaccaa attcgtgcgc gaccggcaag aagctatcgg ggccgatcag ttcatcattc
    54121 tcacctttca tgcgcagaag aatttcatgg taaacatcac cacgcatacc ggtaatcggc
    54181 tgggccatca ggaaaaaatg gttgtgctcc agcgcctgct gtagtcgatt catcatcgcg
    54241 actttatctt tcaattcgcg ttgcaaatac attgccccgc aacgctgcat attttccggg
    54301 gcgttggtca cgatggaaag ttcggcgacc gtatttagct ctcccagcag caggtagata
    54361 tgattcactg gcgagcgcac atagcagtaa ctgacgccaa tctgcggttg catcggcatc
    54421 ccatcccaaa agaaacgaaa ttgcttgaga tgactatcca gtgcggtaat gcgctcctgg
    54481 tgcgattctg tattcagtcg cagcgcgaga tcgttacccg aaagctgata aacatcttca
    54541 cccggttcca gcaagggtga cagccagtga gaaagttttt gcttgt
//
LOCUS       DADLXX010000014        53211 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLXX010000014 DADLXX010000000
VERSION     DADLXX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.014"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(11..322)
                     /locus_tag="JNX27_000799"
     CDS             complement(11..322)
                     /locus_tag="JNX27_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7882101.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JNX27_000800"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JNX27_000800"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JNX27_000801"
     CDS             701..1054
                     /locus_tag="JNX27_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7882102.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNX27_000802"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNX27_000802"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY7882103.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FINVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JNX27_000803"
     CDS             complement(2810..3280)
                     /locus_tag="JNX27_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882104.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNX27_000804"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNX27_000804"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY7882105.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNX27_000805"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNX27_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7882106.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNX27_000806"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNX27_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7882107.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JNX27_000807"
     CDS             complement(5283..8432)
                     /locus_tag="JNX27_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7882108.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNX27_000808"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNX27_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7882109.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JNX27_000809"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JNX27_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7882110.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JNX27_000810"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JNX27_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7882111.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISGLLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNX27_000811"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNX27_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7882112.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNX27_000812"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNX27_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7882113.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JNX27_000813"
     CDS             14911..15288
                     /locus_tag="JNX27_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7882114.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JNX27_000814"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JNX27_000814"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7882115.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JNX27_000815"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JNX27_000815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7882116.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWTAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18099..18428
                     /locus_tag="JNX27_000816"
     CDS             18099..18428
                     /locus_tag="JNX27_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7882117.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JNX27_000817"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JNX27_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7882118.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JNX27_000818"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JNX27_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7882119.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JNX27_000819"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JNX27_000819"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7882120.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JNX27_000820"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JNX27_000820"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7882121.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNX27_000821"
                     /pseudo
     CDS             complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNX27_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /locus_tag="JNX27_000822"
     CDS             24045..25349
                     /locus_tag="JNX27_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7882122.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="JNX27_000823"
     CDS             complement(25482..27158)
                     /locus_tag="JNX27_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7882123.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /locus_tag="JNX27_000824"
     CDS             complement(27396..28616)
                     /locus_tag="JNX27_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7882124.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALPGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /locus_tag="JNX27_000825"
     CDS             28834..30486
                     /locus_tag="JNX27_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7882125.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNX27_000826"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNX27_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7882126.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="JNX27_000827"
     CDS             complement(31206..32000)
                     /locus_tag="JNX27_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882127.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALLVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="JNX27_000828"
     CDS             32138..32479
                     /locus_tag="JNX27_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7882128.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNX27_000829"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNX27_000829"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7882129.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAAFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="JNX27_000830"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="JNX27_000830"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7882130.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVLGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="JNX27_000831"
     CDS             36395..37687
                     /locus_tag="JNX27_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7882131.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /locus_tag="JNX27_000832"
     CDS             37812..38219
                     /locus_tag="JNX27_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7882132.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="JNX27_000833"
     CDS             complement(38220..38678)
                     /locus_tag="JNX27_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7882133.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="JNX27_000834"
     CDS             complement(38675..39592)
                     /locus_tag="JNX27_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7882134.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="JNX27_000835"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="JNX27_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7882135.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="JNX27_000836"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="JNX27_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7882136.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNX27_000837"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNX27_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7882137.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNX27_000838"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNX27_000838"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7882138.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JNX27_000839"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JNX27_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="JNX27_000840"
     CDS             43551..44237
                     /locus_tag="JNX27_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7882139.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46648
                     /locus_tag="JNX27_000841"
     CDS             44234..46648
                     /locus_tag="JNX27_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7882140.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNX27_000842"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNX27_000842"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY7882141.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNX27_000843"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNX27_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7882142.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="JNX27_000844"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="JNX27_000844"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7882143.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="JNX27_000845"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="JNX27_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7882144.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="JNX27_000846"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="JNX27_000846"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7882145.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53211
                     /locus_tag="JNX27_000847"
     CDS             52938..>53211
                     /locus_tag="JNX27_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7882146.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12818 a        13705 c        14023 g        12665 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttta tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatt
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc ggactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tggacggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtattgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccggc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgagca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atgcggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacta ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagtacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt cgcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 gggtcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac caggccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atatccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acggacgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tgtaactatg attttcttca tgtgctagat gaggactgcc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aacaaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ctagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gtaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt taaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgatgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ttggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt c
//
LOCUS       DADLXX010000015        49573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLXX010000015 DADLXX010000000
VERSION     DADLXX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.015"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1224
                     /locus_tag="JNX27_000848"
     CDS             <1..1224
                     /locus_tag="JNX27_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7882147.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JNX27_000849"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JNX27_000849"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7882148.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JNX27_000850"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JNX27_000850"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7882149.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JNX27_000851"
     CDS             2714..3427
                     /locus_tag="JNX27_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7882150.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JNX27_000852"
     CDS             complement(3399..4076)
                     /locus_tag="JNX27_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7882151.1"
                     /translation="MLTLNKISYRWPGAATDCLCGISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JNX27_000853"
     CDS             complement(4070..4747)
                     /locus_tag="JNX27_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7882152.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="JNX27_000854"
     CDS             complement(4735..5118)
                     /locus_tag="JNX27_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY7882153.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="JNX27_000855"
     CDS             complement(5134..5442)
                     /locus_tag="JNX27_000855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882154.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="JNX27_000856"
     CDS             complement(5443..6021)
                     /locus_tag="JNX27_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7882155.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JNX27_000857"
     CDS             complement(6044..6475)
                     /locus_tag="JNX27_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7882156.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JNX27_000858"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JNX27_000858"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7882157.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JNX27_000859"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JNX27_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7882158.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JNX27_000860"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JNX27_000860"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7882159.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /locus_tag="JNX27_000861"
     CDS             10564..11424
                     /locus_tag="JNX27_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7882160.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JNX27_000862"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JNX27_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7882161.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JNX27_000863"
     CDS             12291..13031
                     /locus_tag="JNX27_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7882162.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..13707
                     /locus_tag="JNX27_000864"
     CDS             13198..13707
                     /locus_tag="JNX27_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7882163.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPL"
     gene            13831..14093
                     /locus_tag="JNX27_000865"
                     /pseudo
     CDS             13831..14093
                     /locus_tag="JNX27_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451535.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNX27_000866"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNX27_000866"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY7882164.1"
                     /translation="METQWTRMTADEAAEIILHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNX27_000867"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNX27_000867"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7882165.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNX27_000868"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNX27_000868"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7882166.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNX27_000869"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNX27_000869"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY7882167.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /locus_tag="JNX27_000870"
     CDS             complement(19724..20617)
                     /locus_tag="JNX27_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7882168.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="JNX27_000871"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="JNX27_000871"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7882169.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /locus_tag="JNX27_000872"
     CDS             21915..23147
                     /locus_tag="JNX27_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY7882170.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="JNX27_000873"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="JNX27_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7882171.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="JNX27_000874"
     CDS             23768..23827
                     /locus_tag="JNX27_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7882172.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="JNX27_000875"
     CDS             complement(23924..24526)
                     /locus_tag="JNX27_000875"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7882173.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="JNX27_000876"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JNX27_000876"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNX27_000877"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNX27_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7882174.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="JNX27_000878"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="JNX27_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7882175.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="JNX27_000879"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="JNX27_000879"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7882176.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHARGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="JNX27_000880"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="JNX27_000880"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7882177.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="JNX27_000881"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="JNX27_000881"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7882178.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(29672..29755)
                     /locus_tag="JNX27_000882"
     CDS             complement(29672..29755)
                     /locus_tag="JNX27_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882179.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="JNX27_000883"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="JNX27_000883"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7882180.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="JNX27_000884"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="JNX27_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7882181.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="JNX27_000885"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="JNX27_000885"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7882182.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35555
                     /locus_tag="JNX27_000886"
     CDS             34812..35555
                     /locus_tag="JNX27_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7882183.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVVQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35612..37045)
                     /locus_tag="JNX27_000887"
     CDS             complement(35612..37045)
                     /locus_tag="JNX27_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7882184.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37090..37401
                     /locus_tag="JNX27_000888"
     CDS             37090..37401
                     /locus_tag="JNX27_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY7882185.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            37565..38224
                     /locus_tag="JNX27_000889"
     CDS             37565..38224
                     /locus_tag="JNX27_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7882186.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNX27_000890"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNX27_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7882187.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="JNX27_000891"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="JNX27_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7882188.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="JNX27_000892"
     CDS             39890..40297
                     /locus_tag="JNX27_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882189.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNX27_000893"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNX27_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7882190.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="JNX27_000894"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="JNX27_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7882191.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="JNX27_000895"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="JNX27_000895"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7882192.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="JNX27_000896"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="JNX27_000896"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7882193.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="JNX27_000897"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="JNX27_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7882194.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="JNX27_000898"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="JNX27_000898"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7882195.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNX27_000899"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNX27_000899"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7882196.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            47702..47773
                     /locus_tag="JNX27_000900"
     CDS             47702..47773
                     /locus_tag="JNX27_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882197.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /locus_tag="JNX27_000901"
     CDS             complement(47770..47895)
                     /locus_tag="JNX27_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882198.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47897..49345)
                     /locus_tag="JNX27_000902"
     CDS             complement(47897..49345)
                     /locus_tag="JNX27_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY7882199.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11967 a        12404 c        13348 g        11854 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg tcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgccgcaa
     4021 agacaatccg ttgccgcgcc tggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatttctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 tgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcatt gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgtgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgcaggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ctaggttata
    15961 cgggacaccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttaggcat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gttaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacgcta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccgggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgttggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcac gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagtga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 cgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgctatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 tctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgccatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaggcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agctagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaatcg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accatctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggtatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gatacgttaa ccaccggacg atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac
    35461 aggcagaaga ggtcgtgcag gccattgtct ggctactaag tgataaagcc tcttacgtca
    35521 cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg
    35581 caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca
    35641 cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt
    35701 catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca
    35761 cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg
    35821 cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc
    35881 tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc
    35941 tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc
    36001 agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg
    36061 catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat
    36121 aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc
    36181 cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg
    36241 taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg
    36301 agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc
    36361 gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca
    36421 tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca
    36481 gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt
    36541 tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac
    36601 ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg
    36661 ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca
    36721 gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg
    36781 aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac
    36841 cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact tctttggtga
    36901 tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggtcctttgc
    36961 cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat
    37021 ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga
    37081 aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcttggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggagaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 tggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatagta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    48001 catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    48061 taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    48121 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    48181 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    48241 tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    48301 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    48361 gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    48421 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    48481 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    48541 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    48601 taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc ccggataaat
    48661 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    48721 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    48781 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccagcggccc
    48841 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    48901 gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg gtgataaaac
    48961 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    49021 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    49081 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    49141 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    49201 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    49261 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    49321 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag aataaaaagc
    49381 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    49441 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    49501 aatgacaaga gaacctgata atggaattat ctgtaatttt ctcatattga tatttcatta
    49561 atcattctgg cat
//
LOCUS       DADLXX010000016        49253 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLXX010000016 DADLXX010000000
VERSION     DADLXX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.016"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="JNX27_000903"
     CDS             complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="JNX27_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7882200.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="JNX27_000904"
     CDS             complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="JNX27_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7882201.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRATD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3575..3979
                     /locus_tag="JNX27_000905"
     CDS             3575..3979
                     /locus_tag="JNX27_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7882202.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4005..4718
                     /gene="msyB"
                     /locus_tag="JNX27_000906"
     CDS             4005..4718
                     /gene="msyB"
                     /locus_tag="JNX27_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7882203.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4867..5433
                     /gene="satP"
                     /locus_tag="JNX27_000907"
     CDS             4867..5433
                     /gene="satP"
                     /locus_tag="JNX27_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7882204.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5468..6055)
                     /gene="mog"
                     /locus_tag="JNX27_000908"
     CDS             complement(5468..6055)
                     /gene="mog"
                     /locus_tag="JNX27_000908"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7882205.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6170..7123)
                     /gene="tal"
                     /locus_tag="JNX27_000909"
     CDS             complement(6170..7123)
                     /gene="tal"
                     /locus_tag="JNX27_000909"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7882206.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7402..8856
                     /locus_tag="JNX27_000910"
                     /pseudo
     CDS             7402..8856
                     /locus_tag="JNX27_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            8926..9702
                     /gene="yaaA"
                     /locus_tag="JNX27_000911"
     CDS             8926..9702
                     /gene="yaaA"
                     /locus_tag="JNX27_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7882207.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(9855..10151)
                     /locus_tag="JNX27_000912"
     CDS             complement(9855..10151)
                     /locus_tag="JNX27_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7882208.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10365..11651)
                     /gene="thrC"
                     /locus_tag="JNX27_000913"
     CDS             complement(10365..11651)
                     /gene="thrC"
                     /locus_tag="JNX27_000913"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7882209.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(11652..12584)
                     /gene="thrB"
                     /locus_tag="JNX27_000914"
     CDS             complement(11652..12584)
                     /gene="thrB"
                     /locus_tag="JNX27_000914"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7882210.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12586..15048)
                     /gene="thrA"
                     /locus_tag="JNX27_000915"
     CDS             complement(12586..15048)
                     /gene="thrA"
                     /locus_tag="JNX27_000915"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7882211.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15129..15194)
                     /gene="thrL"
                     /locus_tag="JNX27_000916"
     CDS             complement(15129..15194)
                     /gene="thrL"
                     /locus_tag="JNX27_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7882212.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15408..16094)
                     /locus_tag="JNX27_000917"
     CDS             complement(15408..16094)
                     /locus_tag="JNX27_000917"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7882213.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16494..16634)
                     /locus_tag="JNX27_000918"
     CDS             complement(16494..16634)
                     /locus_tag="JNX27_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882214.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16730..17446
                     /gene="arcA"
                     /locus_tag="JNX27_000919"
     CDS             16730..17446
                     /gene="arcA"
                     /locus_tag="JNX27_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7882215.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17505..18857)
                     /gene="creD"
                     /locus_tag="JNX27_000920"
     CDS             complement(17505..18857)
                     /gene="creD"
                     /locus_tag="JNX27_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7882216.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18915..20339)
                     /gene="creC"
                     /locus_tag="JNX27_000921"
     CDS             complement(18915..20339)
                     /gene="creC"
                     /locus_tag="JNX27_000921"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7882217.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(20339..21028)
                     /gene="creB"
                     /locus_tag="JNX27_000922"
     CDS             complement(20339..21028)
                     /gene="creB"
                     /locus_tag="JNX27_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7882218.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21041..21514)
                     /gene="creA"
                     /locus_tag="JNX27_000923"
     CDS             complement(21041..21514)
                     /gene="creA"
                     /locus_tag="JNX27_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7882219.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21725..22594
                     /gene="robA"
                     /locus_tag="JNX27_000924"
     CDS             21725..22594
                     /gene="robA"
                     /locus_tag="JNX27_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7882220.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22591..23238)
                     /gene="gpmB"
                     /locus_tag="JNX27_000925"
     CDS             complement(22591..23238)
                     /gene="gpmB"
                     /locus_tag="JNX27_000925"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7882221.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23290..23811
                     /gene="yjjX"
                     /locus_tag="JNX27_000926"
     CDS             23290..23811
                     /gene="yjjX"
                     /locus_tag="JNX27_000926"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY7882222.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(23896..24222)
                     /gene="trpR"
                     /locus_tag="JNX27_000927"
     CDS             complement(23896..24222)
                     /gene="trpR"
                     /locus_tag="JNX27_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7882223.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24312..26249)
                     /gene="sltY"
                     /locus_tag="JNX27_000928"
     CDS             complement(24312..26249)
                     /gene="sltY"
                     /locus_tag="JNX27_000928"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7882224.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26460..28127
                     /gene="ettA"
                     /locus_tag="JNX27_000929"
     CDS             26460..28127
                     /gene="ettA"
                     /locus_tag="JNX27_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7882225.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28435..29667)
                     /gene="nadR"
                     /locus_tag="JNX27_000930"
     CDS             complement(28435..29667)
                     /gene="nadR"
                     /locus_tag="JNX27_000930"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY7882226.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(29688..31070)
                     /gene="radA"
                     /locus_tag="JNX27_000931"
     CDS             complement(29688..31070)
                     /gene="radA"
                     /locus_tag="JNX27_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7882227.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31119..32087)
                     /gene="serB"
                     /locus_tag="JNX27_000932"
     CDS             complement(31119..32087)
                     /gene="serB"
                     /locus_tag="JNX27_000932"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7882228.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVLANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32193..32837
                     /locus_tag="JNX27_000933"
     CDS             32193..32837
                     /locus_tag="JNX27_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7882229.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32865..33881
                     /gene="lplA"
                     /locus_tag="JNX27_000934"
                     /pseudo
     CDS             32865..33881
                     /gene="lplA"
                     /locus_tag="JNX27_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            33913..34176
                     /locus_tag="JNX27_000935"
     CDS             33913..34176
                     /locus_tag="JNX27_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7882230.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34337..35056)
                     /gene="deoD"
                     /locus_tag="JNX27_000936"
     CDS             complement(34337..35056)
                     /gene="deoD"
                     /locus_tag="JNX27_000936"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7882231.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35113..36336)
                     /gene="deoB"
                     /locus_tag="JNX27_000937"
     CDS             complement(35113..36336)
                     /gene="deoB"
                     /locus_tag="JNX27_000937"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7882232.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36388..37710)
                     /gene="deoA"
                     /locus_tag="JNX27_000938"
     CDS             complement(36388..37710)
                     /gene="deoA"
                     /locus_tag="JNX27_000938"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7882233.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDH
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(37837..38616)
                     /gene="deoC"
                     /locus_tag="JNX27_000939"
     CDS             complement(37837..38616)
                     /gene="deoC"
                     /locus_tag="JNX27_000939"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7882234.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRYMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            38874..40415
                     /locus_tag="JNX27_000940"
     CDS             38874..40415
                     /locus_tag="JNX27_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7882235.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSL"
     gene            40387..41250
                     /gene="yjjW"
                     /locus_tag="JNX27_000941"
     CDS             40387..41250
                     /gene="yjjW"
                     /locus_tag="JNX27_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7882236.1"
                     /translation="MNSRCALVCKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41464..42246)
                     /locus_tag="JNX27_000942"
     CDS             complement(41464..42246)
                     /locus_tag="JNX27_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7882237.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(42243..43316)
                     /locus_tag="JNX27_000943"
     CDS             complement(42243..43316)
                     /locus_tag="JNX27_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7882238.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43438..43599)
                     /locus_tag="JNX27_000944"
     CDS             complement(43438..43599)
                     /locus_tag="JNX27_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7882239.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(43726..44331)
                     /gene="osmY"
                     /locus_tag="JNX27_000945"
     CDS             complement(43726..44331)
                     /gene="osmY"
                     /locus_tag="JNX27_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY7882240.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(44724..46313)
                     /gene="prfC"
                     /locus_tag="JNX27_000946"
     CDS             complement(44724..46313)
                     /gene="prfC"
                     /locus_tag="JNX27_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7882241.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46404..47081)
                     /gene="yjjG"
                     /locus_tag="JNX27_000947"
     CDS             complement(46404..47081)
                     /gene="yjjG"
                     /locus_tag="JNX27_000947"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7882242.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47096..47542)
                     /gene="rimI"
                     /locus_tag="JNX27_000948"
     CDS             complement(47096..47542)
                     /gene="rimI"
                     /locus_tag="JNX27_000948"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7882243.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47511..47924)
                     /gene="holD"
                     /locus_tag="JNX27_000949"
     CDS             complement(47511..47924)
                     /gene="holD"
                     /locus_tag="JNX27_000949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7882244.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRVNPTARAALWQQICTYEHDFFPRND"
     gene            48027..49058
                     /gene="rsmC"
                     /locus_tag="JNX27_000950"
     CDS             48027..49058
                     /gene="rsmC"
                     /locus_tag="JNX27_000950"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7882245.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        11553 a        13399 c        12428 g        11873 t
ORIGIN      
        1 cgcatatctt caacgcaccc tatttttacc caggcctgcc cacgggcagg cttttgggga
       61 ggttagcggg tcaggtcgtc aaaaaacttc ttcacaccat caaagaagct ctttgagcgc
      121 gggctgttgt gctcgccggt tgggccaccg aagctttctt gcagctcttg cagcagctgt
      181 ttctgctttt cgttcaggcc taccggtgtt tcgacgacaa cgcggcacag caaatcaccc
      241 tgtgcaccac cgcggacaga cttgacgcct ttaccgcgca tacggaatag cttaccggtc
      301 tgggtttcgc caggcacttt cagtttgacg cgaccatcaa gggtcggtac ttcgatttcg
      361 ccacccagcg ccgccatagc gaagttgatc gggacttcgc aatacaggtt gttgccttca
      421 cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt acagatcgcc tgccggtgcg
      481 ccatgctcgc ccgcttcacc ttcgcccgca agacggatgc ggtctccagt gtccacccct
      541 gccgggattt taacggacag cgttttgctg cgctcaacac gaccatgacc atgacatttg
      601 ttgcacggat ctttgatcag cgtaccgcgg ccctgacagt gtggacaggt ctgctgtaca
      661 gcgaagaatc cctggcgcat ctgcacctga ccagaaccat gacaggtcgg acaggtctgc
      721 ggctgtgtac ctggttttgc accgctaccg tggcaaacgt cacactcttc cagagtcgga
      781 atgcggattt ctttggtcac gccacgtaca gcttcttcga gggtgagctc catgttatag
      841 cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg
      901 aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc caaaaccacc gccgcccatg
      961 cctccttgct caaacgcagc atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg
     1021 gtcagaactt cataagcttc cttgatctct ttaaatttcg cctcggcctc tttgtcaccc
     1081 tggttacggt ccgggtggta tttcatggcc aggcgtttgt aggcctttct gatttcacgc
     1141 tcttccgctg ttttggaaac gcctaaaatc tcgtaataat cttgcttagc catctttttt
     1201 aaattgcccc tagatgaatc cacgggcgga gaggaaattc cccttcgccc gtgtcagtat
     1261 aattacccgt ttatagggcg attatttttt gtctttgact tcttcaaatt cagcgtcgac
     1321 aacatcgtca tctttcgcgt tgtttgcaga agcatcagca ccggcagtct gctgctgggc
     1381 atgttgctgc tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt
     1441 cgcttcgata gcggctttgt cttcaccttt cagagcagtt tccagtgcag tcagcgcaga
     1501 ctcgatagca gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa cctgcttacg
     1561 ggagctgtgc agcagatggt cgccctggtt gcgagtctgt accagctctt caaacttacg
     1621 gtcagcttcg gcgttagctt ctgcgtcgcg taccattttc tggatttcat cttcgttcag
     1681 accagaagaa gccttgatgg tgatcttctg ctctttaccg ctgtttttat ctttcgcgga
     1741 aacgtgcagg ataccgtcag catcgatatc gaaggtaact tcgatctgcg gcatgccgcg
     1801 cggtgccggg ttgataccat ctaggttgaa ctgacccaga gatttgttat cagtcgcacg
     1861 tttacgttca ccctgcagca catggatggt taccgcagac tggttgtctt cagcggtaga
     1921 gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac
     1981 accgcccatg gtttcgatac ccagagacag cggggtaacg tccagcagca gtacgtcttt
     2041 tacgtcacca gtcagaacac caccctgaac agcagcaccg attgctacag cttcgtccgg
     2101 gttaacgtct ttacgcggct ctttaccaaa gaactcagca actttcttct gaaccattgg
     2161 catacgagtc tgaccaccaa cgaggataac gtcgtcgata tcagatacgg acaggccagc
     2221 atcctgcagt gcaactttca gcggctcaat ggaacggttt accagatctt caaccaggct
     2281 ttccagtttc gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt
     2341 gatgtatggc aggttaacgt cggtctgctg agcggaagac agttcgattt tcgctttttc
     2401 tgccgcttct ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa tgccctgatc
     2461 tttcttgaat tcttcaacca gatagttgat cagacggctg tcgaagtctt caccacccag
     2521 gtgggtatca ccgttggttg ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat
     2581 ttcgataata gaaatatcga aagtaccacc ccccaggtca taaaccgcga tagtacggtt
     2641 gccagtgcct ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg
     2701 ttttacttcc agaccagcga tacggcctgc gtctttggtt gcctgacgct gagcatcgtt
     2761 aaagtatgcc ggtacggtga taacagcttc agttaccggt tcacccaggt aatcttcagc
     2821 ggttttcttc atttttttca gcacttcagc agaaatctgc ggcggtgcca ttttctggcc
     2881 tttaacttcg acccatgcgt cgccgttatc agcagcaata attttgaacg gcatgatgga
     2941 aacatcacgc tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa
     3001 cagggtgttt tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa ccagagtttc
     3061 accatcctgg gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg cgttctccag
     3121 cacgcgagga atggtgccat ccataatcgc tacacaagag ttggtagtac ccaggtcgat
     3181 accaattatt ttacccatct aaacgtctcc actatatatt cggtcatcat gtggttgtga
     3241 gtctgtaata ggggcgaaac gtctggtttc aactgcccaa tttcattttt ttgcagactc
     3301 actcactgcg gttgactact aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa
     3361 aaattttttg tgcttttgcg cagaaatttc gcgatatggc aggacaagac gtaaaattga
     3421 cagcaatttt attcatataa agaatgaatc gcagccaagg cattcatcaa aaaattgtaa
     3481 taaaaagaaa agattacgtg cctgaatctt ctctttatca gcagtaaact agtgggtatt
     3541 catcccccta cctcttccca ctaagagaat ccttatgaaa tccgttttta cgatttccgc
     3601 cagcctggcg attagcctga tgctgtgctg cacggcgcag gcaaacgacc ataaactcct
     3661 cggcgtcatt gcaatgccgc gtaacgaaac caacgatctg gcgctgaaac ttcctgtttg
     3721 tcgcattgtg aaacgcatac aactctctgc cgaccatggc gatttacagt taagcggtgc
     3781 atcggtttat ttcaaagccg cccgtagcgc cagtcagagc ctgaatattc cttcagaaat
     3841 aaaagaaggg caaaccactg actggatcaa cattaacagc gataacgaca ataaacgctg
     3901 cgtctcaaaa atcacctttt ccggtcatac ggtgaactca tcggatatgg ccacgctgaa
     3961 aattatcggc gacgactaac gccagaatat agaagccaca aaaaatgaat gttaattacc
     4021 tgaatgattc agatctggat tttcttcagc attgtagtga ggaacagttg gcaaatttcg
     4081 cccgattgct cacccataat gaaaaaggca aaactcgcct ctccagcgta ctgatgcgca
     4141 acgaactgtt taaatcgatg gaagggcatc ccgagcaaca tcgccgcaac tggcagctga
     4201 ttgccggaga attacagcat tttggtggcg atagtatcgc caacaaactg cgcggacacg
     4261 gtaaattgta tcgggccatt ttgctcgatg tttcaaagcg attgaagctg aaagccgaca
     4321 aagagatgtc tacgtttgaa attgagcaac agttactgga acaatttctg cgtaatacct
     4381 ggaagaaaat ggacgaggaa cataagcagg agtttctgca cgcggtcgat gccagggtga
     4441 atgagctgga agagctgctg ccgctgctga tgaaagacaa attattggca aaaggtgtgt
     4501 cgcatttgct ttccagccaa ctgacccgca ttttacgcac ccacgcagca atgagcgtac
     4561 ttgggcatgg tttgctgcgc ggagcggggc tgggaggccc tgtgggcgca gcattaaatg
     4621 gggttaaagc ggtcagcggc agcgcctatc gcgtgacgat tccagccgta ctgcaaatcg
     4681 cctgcctgcg ccggatggtt agcgccactc aggtctgata ccggaaaccg gggaaaattt
     4741 tcattttccc ctcaaaagat cgtagacact gccccactgg ctgattatta tgccgcgccc
     4801 tgaaaacact acagttattc agggaaatta tttcaccatt cattcgatga tgatttttga
     4861 ggaattatgg gcaacactaa gttggctaat ccggcaccgc tgggcctgat gggcttcggc
     4921 atgaccacca ttctgcttaa cctgcacaac gtgggttatt tcgctctgga cggtattatt
     4981 cttgccatgg gcattttcta cggcggcatc gcgcaaattt ttgctggtct gctggagtac
     5041 aaaaaaggca acactttcgg tttaaccgca ttcacctctt acggttcttt ctggctgacg
     5101 ctggttgcga ttctgctgat gccgaaactg ggtctgaccg atgcgccaaa tgcacagttc
     5161 cttggtgtct acctgggtct gtggggcgta tttacgctgt ttatgttctt cggcactctg
     5221 aaaggcgcac gcgtcctgca attcgttttc tttagcctga ccgtgctgtt tgccctgctg
     5281 gcgatcggta acattgccgg taatgccgcg atcatccact ttgccggctg gattggtctg
     5341 atctgcggtg ccagcgcaat ctatctggcg atgggtgaag tgctgaacga gcagtttggt
     5401 cgcaccgttc tgccgattgg tgaatcccac taattgtctt gagatccccc cgctcggggg
     5461 gattttttta ttcgctaacg tcgcgtcttg cactcttcgg tctgaatgca gcaaccactt
     5521 ctggtgccgt ttcaacgtat ggcccttcca gcaactgaat gcagtacggt acgctggcaa
     5581 aaataccatg caccacaacg ttaccctcag cgtccttcac accttccagc gtctctttaa
     5641 tagacttcgg ctgaccgggt aagttaagga tcagcgcctg tttacgaatc acgcccacct
     5701 gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat ctggcgcatc tgttcaccaa
     5761 agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc gggcgttacg tcacgacgcg
     5821 ccgggccagt tccgcccgtg gtgagcacca gatggcaact catttcatcc accagctcac
     5881 acaacgtttg ctcgatgatc gcctgctcat cggggattaa gcgggtttcc agttcaaacg
     5941 gcgtggttag cgccgatgtc agccattctt ccagcgctgg aatgccttta tcctgataaa
     6001 cgccgctgga tgcgcgatca gagatggaaa ctaagccaat acgtaaagta ttcatcaaga
     6061 tttttccgtt taaacagtct cgttaaacag aatgatacac tgcgaaggga gtgacagaca
     6121 ggcttcagaa gaggtagcgt gaccgacttc ccggtcacgc taagaatgat tacagcagat
     6181 cgccgatcat tttttccagt ttttcctggt caatagcaaa cttacggata ccttccgcca
     6241 gtttatctac tgccattgga tcctggttgt gctgccacag gaactcggac tcagtgatac
     6301 gcgccggacg cgctttcact tcgccggtgt aagacagttt acgttcgata gccccttcgc
     6361 tctccgccag ctctttcagc agtgccggtg cgatggtcag acggtcacag cctgccagtt
     6421 ccagaatttc gccgatgtta cggaagcttg cgcccataac cacggtttca taaccgtgct
     6481 ctttgtagta ctggtagatt tcagatacag aaaccacgcc cggatcttct gccggagcgt
     6541 actctttctt atcggtattc gctttgtacc agtcaagaat acggccaaca aacggcgaga
     6601 tcaggaacac gcccgcctcc gcacaagcac gagcctgagc gaaggagaac agcagggtca
     6661 ggttacagtt gataccttct ttttccagct gttctgcagc acggataccc tgccaggtag
     6721 aagccagttt gatcagaata cgatcgttgc taatacctgc atcgttgtag agtttgatca
     6781 ggcgttttgc tttcgcaatt gacgcttcgg tgtcatagga aagacgcgca tcaacttcag
     6841 ttgagatacg gcccggaacc agtttcagga tttccagacc aatatttact gccagtttgt
     6901 cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg ctgtttcgcc caggcgacag
     6961 catcatcaat caacttacgg tattccggaa tctgcgctgc gttaagaatg agagaagggt
     7021 tggttgtggc atcctgcggt tgatacagct tcattgccgc gatgtcccca gtgtcggcca
     7081 ctacggtggt gtactgacga agggaggtca atttgtccgt catgatagta tttctcttta
     7141 aacagcttgt tagggggatg taaccggtct gccctgatga tatcacgacg caacgtcagc
     7201 gcaaccgccg acaagacggt tatcgccagg cgagactgtt tcggatttct gacactgcgt
     7261 tgatatcgcc cgccattttt atacaaaacc tcatgtatgc tacgcagaag ttatcaagta
     7321 cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt acacaggaaa gtcatcgcga
     7381 ccggcaataa gagggatatg catgccagat tttttctcct ttattaacag cgtcctttgg
     7441 ggatcggtaa tgatttacct gctcttcggc gcaggttgtt ggttcacttt tcgcaccgga
     7501 tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc ttaaaaatag cattcatcca
     7561 cagccaggcg gtttaacctc atttcagtca ttgtgtacca gtcttgcggc gcgcgtgggt
     7621 agcggcaacc tggccgccgt tgcgctggca attaccgccg gtggaccagg tgccgtcttt
     7681 tggatgtggg ttgctgcgtt tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca
     7741 caactttata aagaacgtga cgtcaatggg cagtttcgtg gcggaccggc atggtatatg
     7801 gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg ccgtcttttt gctcatcgcc
     7861 tacggcataa ttttcagcgg agttcaggcg aacgccgttg cccgagccct gagtttttct
     7921 tttgattttc ctccgctggt gacgggcatt attctcgctg tctttgctct gctggcaatc
     7981 actcgcggtc ttcatggcgt cgcccggctc atgcagggtt ttgtcccgtt gatggcgata
     8041 atctgggtac tgaccagcct ggtgatttgc gtaataaata tcgggcaact tccccacgtc
     8101 atttggtcta tttttgagag tgcttttggc tggcaggaag cggcaggcgg cgcggcggga
     8161 tataccttaa gccaggcgat tactaacggt tttcagcgca gtatgttttc caatgaggcg
     8221 ggaatgggat cgacgccaaa cgcggcagcg gcagcggcag cggcagcggc agcggcagct
     8281 gcatcctggc ctccgcatcc ggcagcacaa gggattgtcc agatgattgg catttttatc
     8341 gacaccctgg tcatctgtac ggcaagcgcc atgctgatat tactggcggg taacggcaca
     8401 acctacatgc cgctggaagg tattcagctt atccagaagg cgatgcgggt gctaatgggt
     8461 tcctagggtg ctgaatttgt taccctcgtg gttattctgt ttgccttcag ctccatcgtt
     8521 gccaactaca tttatgccga aaacaatctc ttctttttac gcctgaacaa ccctaaagcg
     8581 atctggtgtt tgcggatctg caccttcgca acggtcatcg gcggcacctt gctaagcctt
     8641 ccgctgatgt ggcaactggc agatatcata atggcctgca tggctattac caatttgacc
     8701 gccattttac tgctctcgcc tgtggttcat accattgcca gtgattatct acgccagcgt
     8761 aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc cggatatcgg tcgccagctt
     8821 tctccggacg cgtgggatga tgtttcgcag gagtaatcac aactatcgat caactcattc
     8881 tctttttttg ctaaagtcgg cataaatttc ctgcaaggac tggatatgct gattcttatt
     8941 tcacctgcga aaacgcttga ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg
     9001 gagctgttag acaattccca gcagttgatc catgaagcgc ggaaactgac gcctccgcag
     9061 attagcacgc tgatgcgcat cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat
     9121 gactggcagc cagatttcac gccggcgaat gcccgccagg cgattctggc gtttaaaggt
     9181 gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa
     9241 cagcatttgc gaatgctttc cggcttgtat ggcgtactgc gcccgctcga tttaatgcag
     9301 ccttatcgtc tggaaatggg gatccgtctt gagaatgccc gagggaaaga tctgtatcaa
     9361 ttctggggag atatcatcac caacaagctg aacgaggcgc tcgcagcaca aggcgataat
     9421 gtggtgatta atctggcgtc agatgagtat tttaaatcgg tgaagccgaa gaaattgaat
     9481 gccgagatta tcaagccggt gttcctcgat gagaagaacg gcaaatttaa gatcatcagc
     9541 ttctacgcta agaaagcacg cggtctgatg agtcgtttca ttattgaaaa tcggctgacc
     9601 aaaccagagc aactgactgg ttttaatagc gaaggttact tctttgatga agattcctcc
     9661 agcaatggcg aactggtgtt taaacgctac gagcagcgtt aagtttgtag tcaatgccgg
     9721 atgcggcgtg aacgccttat ccggcctaca aaagcatgca gattcaatat attgcagaat
     9781 taacgtaggc ctgataagac gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac
     9841 ccggcatttg tcatttagcg gtgatgtttg cctggaccat gaccgccgtg atgatcatga
     9901 ggagctttct tatggtggcg cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct
     9961 cgccattcat aatgttgttt ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa
    10021 tagccacgat tatcacgatc gcctatctgt aattttactg acgggactaa cgtaatttcc
    10081 gcagcctgtg ctgccatggg agcgaccaga accagggaaa gtgcgagtac gatagattgc
    10141 atctttttca caggttactc cttttatcat tgcccgttat gggtcgatat ccggagatta
    10201 cgcgatgaga acgacggttg ttattctccg caatcctaat ctctaagtta ccgcatttat
    10261 tgataatttc tcctttttcc ctgtcgtttt tctccataat ttcttcataa aaaagccggg
    10321 ctgcataaaa gcaaacccgg cctgattgag ataatgaata gattttactg atgattcatc
    10381 atcaatttac gcaacgcaga aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca
    10441 gcacgttctg ccagctcttt tggcagatcc aacgtttcac cgagaatcgc ttccacgctc
    10501 tctttaaatt tcgccggatg cgcggtgccg aggaacaagc catattcgcc tggattcaac
    10561 tggtcacgca gcgcacgata cgctacggca gcgtgcggct ccgaggtgta gcccagttct
    10621 tttaactcac gcattgtctg ttgcgtggtt tcatcatcca cggctgcata acccagctct
    10681 ttcagttgcc agattttgcg gcggaacaac tcttccacac gcggccagtt gttcggctga
    10741 ctcacgtcca tcgcgttgga taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg
    10801 tgcaggaaac gtggcacggt atcgttcacg ttggtcgcag caataaaacg tttcaccggc
    10861 agaccgaatg acttcgccag cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc
    10921 gagacaacca gctggttgcg cgcctcctgc ggcagctgcg caacagcttc aaagtagtag
    10981 caaatctgcg ccagcaaacg gctgatgtta atcgagttag ccgagtttaa ccccagcgcc
    11041 actttcagtt cttcatcatc aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg
    11101 ccgtcgatgg caacagtttc gatattgccg cccaacgtac agaacagttt ttcttgcagt
    11161 ggactgattt tgcctcgtgg atagaggata accactttca cattcggcaa accgtagaaa
    11221 gcatgagcca ctgcagctcc ggtatcaccg gaggtcgcgg tcagaatggt cactggctta
    11281 tcatccgcaa tatgggtcag catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc
    11341 agcgttggcc cgtggaacaa ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga
    11401 gccgggaagg caaacgccgc gcgcacgcgc tcttccagga tttcctgtgg gatttcatca
    11461 ccaataaacg ccgagaggat cttcgcactg cgggtgacaa aatccagctt cagcatctca
    11521 tcaatttcag tcaggctgaa ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg
    11581 cccaacccct gggttacggc ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg
    11641 tagagtttca tttagttttc cagtactcgt gcgcccgccg tatccagccg gcaaatatga
    11701 acaaaacctt cctgattttg caggtagttc ttacccaacc agtcggcaac gcgctgggcg
    11761 gtatccggct tgtcacatag agcgaacaag gtcgggccgg agccggagat accgctcgct
    11821 accgcgccga tttccgcgac cgcctgccgc gcctgccgga agccaggcag taaccgttca
    11881 cggtagggtt cagcgataac atctttcatc agcttcgcgg caagctcagg ctgacgggaa
    11941 tagcaggcgt gaatgaagcc agccagatgt cgcccgtgcg caatgcaatc ctggcggcga
    12001 tactgcgccg gtaaaatagc ccgggcttct gccgtcgaga ctttaattcc cggatacgcc
    12061 agcacccaca gccactcatc aaaccctggc acttgctggc tgatgatgtc gttttcttcg
    12121 atcatcaact gcataccacc aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg
    12181 gagatacgtc cttccagctc gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg
    12241 ccgcagtgtt cattcatcgc catcagcgcc gcgaccaccg aacaggcgct ggagcctaag
    12301 cccgaaccga tcggcatatt cttttccaga gtcatcgcca ctggaatttg cttgccaagc
    12361 tcctggcaaa aacgctccca gcactgataa acgatatttt cccgtggttc tgacggcagc
    12421 ttatcggcaa agcgtccgag gttgttgaga ctgaatgtct ctgccgcctc aaccgtgact
    12481 acatctccga gcaatgcacc atcaacgggt gccaccgccg ccccgagcac atcaaacccg
    12541 acgctcatat tggcactgga agccggggca taaactttaa ccatgtcaga ctcctaactt
    12601 ccatgagagg gtacgtagca gatcggcaaa gacaccggca gcggtaacgt cattgcccgc
    12661 accatatccg cgcagcacca acggcagcgg ctgataatag tggctataaa aggccagggc
    12721 gttttcgcca tttttcactt tgaacagcgg atcattccca tccacttcgg caatcttcac
    12781 gcggcagaca ccatcttcat caatattgcc aacatagcgc aaaacttttc cttcatcacg
    12841 ggccttcgcc acgcgcgcgg caaagagatc gtcgagctgt gacagattcg ccataaaagc
    12901 ggcaacatca ccctcggcgt taaactctgc gggcagcaca ggttcaattt caatatccgc
    12961 cagctccagt tcacgtcccg tttcacgagc gagaatcaat agtttacgcg ccacatccat
    13021 accagaaaga tcatctcgcg ggtccggttc ggtataaccc atttcccgcg ccagcgtggt
    13081 cgcctcggag aaactcatgc cttcgtctaa cttgccgaag atataagaaa gcgaacctga
    13141 aagaatgccg gagaacttca ccaattcatc acctgcattg agcagatttt gcaggttctc
    13201 aataaccggt aatccagccc caacgttggt gtcatagagg aatttacgcc gcgatttttc
    13261 cgccgcataa cgcaactgat gatagtaatc catcgacgag gtgttggcct ttttgttcgg
    13321 cgtgacaacg tggaaacctt cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct
    13381 ggaggtgcag tcaacaatga ccgggttcag cagatgatat tctttcacga ggcgaattaa
    13441 gcgcccgaga ttaaacggct ctttggcttg tgcaagttct tcctgccagt tttccagatt
    13501 aaggccatgc acactagtga gcagagcctt cgagttggca acgccgcaga cacgtaagtc
    13561 gatatgttta ttcttcagcc agctttgctg acgcttcagt tgctccagca gcgcaccgcc
    13621 aacgccaccg acgccaatca caaacacttc gataacctga tcggtattga acagcatctg
    13681 atgagtaacg cgcacgccag tggtcgcatc atcgttattt accacgacag agattgagcg
    13741 ttcagaagat ccctgagcaa tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa
    13801 gaatttcgcc gagatcccac gcaaggtgcg cataccatca cctacaaccg agataatggc
    13861 cagccgttcc gtcaccgcca gcggctccag taagccttct ttcagttcca ggtagaactc
    13921 ttcctgcatt gcccgttcag ctcgcacaca gtcgctttgt ggaacgcaga aactgatact
    13981 gtattcggaa gatgattgcg taatcagcac cacggaaata cgggcgcgtg acattgcagc
    14041 aaagacgcgc gccgccatgc caaccatccc tttcatcccc gggccggaaa cgctgaacat
    14101 tgccatgtta ttcagattgg aaatgccctt gaccggtaat tcgtcttcat cacggctggc
    14161 accaatgagc gtacctggtg cttgaggatt tccggtattt ttaatcaggc aagggatctg
    14221 gaactgggcg atgggggtaa tggtgcgggg gtgaagaact ttagcgccga agtaggaaag
    14281 ctccatcgct tcctggtagg acatcgactt caacaacctc gcatcgggca cctgacgcgg
    14341 gtcgcaggta tagaccccgt caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca
    14401 ggcagccagc accgccgcgg agtagtcgga accgttacgt ccaagcacca ccagttcgcc
    14461 tttttcatta ccggcggtga aacctgccat caacaccatg tgatcagccg gaatgcggct
    14521 tgccgcaata cggcgggtgg actcagcaat atcgacggta gattcgaggt aatgccccac
    14581 tgccagcagt ttttcgaccg gatcgataac ggtaacgttg tgaccacgcg cttctaacac
    14641 gccggccata atggcgatcg acattttctc gccacggcaa atcagcgcag cgttgatgct
    14701 atccgggcac tgccccaaca aactaatgcc atgcaggaca tgttttattt gggcaaattc
    14761 ctgatcgacg aaagttttca attgcgccag cgggaacccc ggctgggcgg cggcgagtcc
    14821 cgtcaaaagt tcggcaaaaa tacgttcggc atcgctgata ttgggtaaag catcctggcc
    14881 gctaatggtt ttttcaatca tcgccaccag gtggttggtg attttggcgg gggcagagag
    14941 gacggtggcc acctgcccct gcctggcatt gctttccaga atatcggcaa cacgcagaaa
    15001 acgttctgca tttgccactg atgtaccgcc gaacttcaac actcgcatgg ttgttacctc
    15061 gttacctttg gtcgaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc
    15121 tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga tggtggtggt aatggtggtg
    15181 ctaatgcgtt tcatggatgt tgtgtactct gtaattttta tctgtctgtg cgctatgcct
    15241 atattggtta aagtatttag tgacctaagt caataaaatt ttaatttact cacggcaggt
    15301 aaccagttca gaagctgcta tcagacactc tttttttaat ccacacagag acatattgcc
    15361 cgttgcagtc agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata
    15421 tttttttcaa tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc
    15481 cccaggcgtt gctgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc
    15541 agcaatgcca tgactcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca
    15601 cttttcgccg gttgttgtat taatgttgct aattgatagc aatagaccat caccgcctgc
    15661 cccagattga gcgaaggata atccgccacc atcggcacac cagtaagcac gtcagccaac
    15721 gctaactctt cgttagtcag cccggaatct tcgcgaccaa acaccagcgc ggcatggctc
    15781 atccatgaag atttttcctc taacagcggc accagttcta ctggcgtggc gtagtaatga
    15841 tatttcgccc gactacgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca
    15901 gccagtgtcg ggaaaacttt aatattatca ataatatcac tagatccatg tgcgacccag
    15961 cgggtggctg gctccaggtg tgcctgacta tcgacaatcc gcagttcgct aaaacccatc
    16021 gttttcattg cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccaggata
    16081 atcgttatac gcatattgcc actcttcttg accaaataac cgcgaacagg gtgatcactg
    16141 tcaacttatt acgcggtgcg aatttacaaa ttcttaacgt aagccgcaga aaaagccctt
    16201 tacttagctt aaaaaaggct aaactatttt ctgactgcac taacggttta attgttaaaa
    16261 aatgctacat atccttctgt ttacttagga taattttata aaaaataaat ctcgacaatt
    16321 ggattcacca cgtttattag ttgtatgatg caactagttg gattattaaa ataatgtgac
    16381 gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac aattgaactt
    16441 gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc ctgttatgtt
    16501 gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg agggaaagta
    16561 cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta
    16621 cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag gcaggtcagg
    16681 gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca tgcagacccc
    16741 gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa gtattttcga
    16801 agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc agatcctctc
    16861 tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga acggtcttct
    16921 gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga ctggccgtga
    16981 caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca tcaccaaacc
    17041 gttcaacccg cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct
    17101 gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg gttgggaact
    17161 ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc tgccgcgcag
    17221 cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt ctcgtgctga
    17281 actgctgaag aaaatgaccg gccgtgagct gaaaccgcac gaccgtactg tagacgtgac
    17341 gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa tcatcgccac
    17401 cattcacggt gaaggttatc gcttctgcgg tgatctggaa gattaatcgg ctttaccacc
    17461 gtcaaaaaaa cggcgctttt tagcgccgtt tttatttttc aaccttattt ccagatacgt
    17521 aattcatcgt ccgttgtaac ttctttactg gctttcattt tcggcagtga aaacgcatac
    17581 cagtcgatat tacgagtcac aaacatcatg ccggccagcg ccaccaccag cacactggtt
    17641 cccaacaaca gcgcgctatc ggcagagttg agcagtcccc acatcactcc atccagcaac
    17701 aacagcgcga gggtaaacaa catgctattg cgccagcctt tcaataccgc ctgcaaataa
    17761 ataccgttca ttaacgcccc aatcagactg gcgattatcc atgccacggt aaaaccgata
    17821 tgttcagaaa gcgccagcaa gagcaaataa aacatcacca atgaaagccc caccagcaaa
    17881 tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa
    17941 gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt ccgttaattg gtattgatcg
    18001 gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt cccagccggt atcattgcct
    18061 gaagcaaaac gctcaccgag attattagca aaccagctgc tttgccattg cgcctgaaaa
    18121 cctgactcgc tagcttcccg tttggctggt agaaaatcac ctaaaaaact gggatgcggc
    18181 cagttgctgg ttaaggtcat ttcgctatta cgcccggcag gcaccacaga aagatcgccg
    18241 gtaccgctta aattcagtgc catattcagc tgcaggttct gcttccgcca gtccccttcg
    18301 ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc
    18361 agtgccgttc cgttaacttc aggcgctttc accacaccaa taccacgcgc atccccgacg
    18421 ctaatcacaa taaagggctt gcctaagatg atatttggcg cgttgagctc gctaagacgc
    18481 gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc agacctgacc ggtataaatc
    18541 cctatcttgc gttcttccac gttctgattg ccatcaacca tcaatgactc aggtaaccaa
    18601 aaatggataa aacttcgttt ccgctccacg gttttatcct cttcctgcac cgtataaagc
    18661 tcggtcacag gaatagcgat aagcggccca acgagttttt gcggcccgct ggtactttgg
    18721 cgaatcgcat cttccacatc gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc
    18781 agcattatcg gaatcaacaa cagcaatact gcaccaaaca aggtggtcat tttccagaac
    18841 aggggggatt tcaacatagt cttctccttt gcaatggcag caggatacga aggctatgtg
    18901 ggaagaattt gaagctatgt gaagttacgg tgaagtcgta gcgaggccag cacgccacct
    18961 tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc actgacgaac
    19021 gccaacccca gaccgctgct tttttgccca tttgcacgcg gcaaagagta aaagcgttca
    19081 aaaatacgtg acagcgcgta gtcaggaata ccactaccgg tatccagcac cttaagcgtg
    19141 acgtgttcct gatccacttc ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg
    19201 atggtgttat ccagtaaatt ccccagcgcc tgatccagta acgccggttc agcagcaaca
    19261 ttaacctcgg tgggcataac atgcaaagtg atgttttttt ctgccaactg cacggtgcgc
    19321 gcttcgctga cgcggcgaaa taatgccgcc acatcaacag cagtcagaac gacttcctga
    19381 cgattctcca gtcttgcctg gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt
    19441 tgtgtcagaa tgttgtcagt aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa
    19501 atttccgccg cgccacgaat cgccgccagt gggcttttta gctcatgggt taacgcataa
    19561 acatactgct caatatagtt tttcccttcc agcttcacgc gcatactttc cagcgcctgc
    19621 gcgagtttac gcagctcgct actaccgaga tcgggaagag gaacgggctt attgtcagtg
    19681 acggaatcag catagcgagt gagccgggca atggagcggt tgatccacca aaccatgcct
    19741 gcgccaatca ccagtgcaat ccccaacaaa atggcgctgg cccataaaat tcgccgctcg
    19801 ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa aacgccaata
    19861 agccgcgagc cgcccataat cggcgcggca acatacatca ccgaactttc gggatcggca
    19921 ggattttgca acgtgctgcg cgcaccatac tgaccacgca acgttagcca gacgtcattc
    19981 cagcgcgaat aatcctgtcc aacggcttta tttgccgaat cgaacaatac tttgccatgc
    20041 gcatcggtca tatagacatg atattcattg cgcactttgt taatgccacc aatattggcg
    20101 cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc
    20161 ccagagagca aatcgggacg cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc
    20221 ccctccgttg ctcttcgcac gcccggttta acttctttga caaaaatagc cagtacgaac
    20281 caggccgcca ccgccaccag taaaaaatag cccagcagca accgcatgcc gatacgcatt
    20341 acaagcccct caggctatat cccatgccgc gatgagtatt aatcggtgaa agatcggggt
    20401 tgatggcgcg cagcttggca cgcagcgttt taatgtggat atcgacggtg cgatcgtagg
    20461 tgtcctgcgc atcttcccac acgctatcca ttagttgctg gcgggaccag acgcggcctg
    20521 gcgacttgag taacgtcttc agcaataaaa actcataccg cgtcagcgtt aatggcgtgt
    20581 caaaccagct gatctgcgcc gcgggttcat tcaattcaaa atgaccaata cggatgacgg
    20641 gagacggcgt cgagaacttc ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt
    20701 cgcggggtga aaacggttta gccacatagt cgtcaacgcc aatttccagc ccaagcaggc
    20761 gatcgacctc ttcacttcgg gccgtcagga acagtacagg taacgccgga tggagcgcca
    20821 gtaactggcg gcacaattca aagccactga tatcaggcaa accaacgtcg agaatcatga
    20881 cgtcgggaac ctgctggcga gctttatcca gcaccggcaa acctcgctca aagacctcga
    20941 cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc ggctatccct tgctcatctt
    21001 ccactaacca gaccgtttcc cgttgcataa atcgcctctg ttattgccgc cacggcatga
    21061 caggaaccgc gctaatcgcg tttttcggcg aaccttctac aactttgtcg gagtaagcca
    21121 gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga
    21181 ccagggacgt gcgttttttg aatactacct cgccctgagc tttgccgttt ttaatacgat
    21241 ccgacagttc aatcggcccg acttgctgac aagaaatggc cgcatcggag gtatcttctg
    21301 ccaggcccaa tcccccttta ataccaccgg ttttcgcccg gctcacataa caggtgacat
    21361 ttttcacatc gggatcgtca aaggcttcca caacaatttt gtgatccggg ccgatcattt
    21421 taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc agcattatca
    21481 ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt accattttta tctattacac
    21541 gagcaattag ttcgtcacgg taaataacat attgattaag aacaaaatct caattctttt
    21601 atttccgtca ggccctaaaa catactctac taagaaaaaa acactgaatg ctaaaacagc
    21661 aaaaaatgct attatccaat tacctgatgt caggtgctcg ttgttgaaag gatgaggata
    21721 ttttatggat caggccggca ttattcgcga ccttttaatc tggctggaag gtcatctgga
    21781 tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt tattccaagt ggcacttaca
    21841 gagaatgttt aaagatgtca ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt
    21901 gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg attctggaca tcgcgctgca
    21961 ataccgcttc gactctcaac agacatttac ccgcgcattc aagaagcagt ttgcccagac
    22021 tcctgcactt taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg
    22081 tctgggtaaa ttcacaatgc cagagcacaa atttgtcacc ctggaagata cgccgctgat
    22141 tggtgttacc cagagctact cctgttcgct ggagcaaatc tctgatttcc gccatgaaat
    22201 gcgttatcag ttctggcacg attttctcgg caacgcgccg accattccgc cggtgctcta
    22261 cggcctgaat gaaacgcgtc cgagtcagga taaagacgac gaacaagagg tattctatac
    22321 caccgcgtta gcccaggatc aggcagatgg ctatgtactg acggggcatc cggtgatgct
    22381 gcagggcggc gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt
    22441 tatcctgacg gtatacggaa cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca
    22501 ggatattgag cgatactacc cggcagaaga cgccaaagcg ggagatcgcc caattaatct
    22561 gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc agctcatcta atgcaggggc
    22621 gtccagatgc gagatgtccc ctgcggtttc aacgacccag ccagacgcca gccacaggct
    22681 ttcctgataa tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc gctctgccca
    22741 tgctggtaat ccgagaatcg tactcaccag acatcccagt gcaataccat ggctcaccag
    22801 cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc
    22861 gctgagctcc tgcattgact cgccttcagg aatacgcccg tcaacggtgc cattgaccag
    22921 ctgccgacgc cagttctctt cttcttcggt cagagaatcg atatgtcttt tttccagcac
    22981 acccatgttt aattcacgca ggcgagaatc aaagatgatg tcacagccgc aggcctgggc
    23041 gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc
    23101 aagctctttg gcacgggttg ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg
    23161 gctgtcagac tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac
    23221 taggtatacc tgtaacatgc tttttttccg tatactgctg taacgttaat tttctgagta
    23281 atgctgatta tgcaccaagt tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt
    23341 ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc
    23401 gagagcggtg taccggaaca accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat
    23461 cgggtagcaa atgcccgccg tttacttcca gaggctgatt tttgggtggc gattgaagct
    23521 ggcattgatg gcgacagcac tttcagttgg gtggtgattg aaaacgccag ccagcgcggc
    23581 gaggcgcgtt cggcaacttt gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc
    23641 gaggcgcttg gtcccgtgat gtcgcgttat accggtattg atgagattgg tcgtaaggaa
    23701 ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta tcaccaggcg
    23761 gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc aggcattgta ggacggataa
    23821 ggcgttcacg ccgcatccga cattcggtgc acgatgcctg atgcgacgct gccgcgtctt
    23881 atcaggccta caaaatcaat cgcttttcag caacacctct tccagccact gacgcagctc
    23941 cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc ccgcgccgag
    24001 ttcatttttt aactcacgct gactcatttc gccacgcaac agctcttcga caatacgcac
    24061 gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca acggtaaatg
    24121 gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg
    24181 ctgttctgcc atcgctgctg aatagggtga ttgttgggcc ataatatgtc gccattgtta
    24241 gcgggggaag caaaatgact cccccggttg tactcgctaa agagtacgat agcatatcat
    24301 aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc tcatcaacgt cggtttatcc
    24361 cccatgaaat agcggtagta agcatcataa gccagcacgt tcttcacata accgcgcgtc
    24421 tcggagaacg gaatactctc gacaaatgcc acagcgtcga tacgcccggc gctgttgcca
    24481 agccaggttc gcacccgtcc aggtccggcg ttataagctg ctgaggagaa aatacggtta
    24541 ttgccaaact gctgataaac atattgcagg taactggtgc caatgttgat gttcgtttcc
    24601 ggatccagca attgcccagg gctgctataa ccaggaatag agaacatctt caccgtatgg
    24661 gtcgctgtac caggcataat ctgcatcagg ccgctggccc ctaccggtga tttcactttc
    24721 ggattccagg cgctctcctg acgggcaatc gccatcgcat agctttgcgg gatctctttc
    24781 ccactggtgt aacgtttgaa aagatcgttg taagccagcg ggaatcgctc ttccagatga
    24841 tcccaaagct tcccggcgat cgttgcctga acgctaagat cccaccattg gttattgaaa
    24901 gcataccgag ccagttgagc ctgctctgtt tttgacttgc tcttcaccag attggcccac
    24961 tcgctacgcg cggtattatc gagattccag tacatcaact cgcgcacgcg cgccatctcc
    25021 ggcccctgag tcagggtgct gtcaacattc tgcggcgctt tatcaatctt cagctcatac
    25081 tcttcaccga tgcgttgtgc tgcaaccatc gggtagaaac cacgctgttg catgagttga
    25141 tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca gcaataaatc cgcctgccag
    25201 taacgccatt catctttctc ttttgcttcc atcggcagac gcgccagcca ggtattcagg
    25261 ccgcgacgat cgccggtgcc aagcgccatt cgcacacggc gttcaatcaa cgacgtggat
    25321 tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct catcggtgac atcattgccc
    25381 atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct gatcttcatt aagctgctgc
    25441 gcctgggcaa gcgacgggat catcagccgt gcattttcgg catcctgccg cgccacactg
    25501 gcaaacgcca ccgccgccat ttgacgggta aaatcggtcg cgccggttgt acgcgcgaag
    25561 gtcagtaccg tattagggtt gttcgccagt gaaatgattg ccgaggcgat agtctggtaa
    25621 tcggcaggca tctgccctgc cagcactgtt accaggcctg tgttacccgc tttcatcgcc
    25681 agacggatac gctctaaata cgccagcgga tcttgtttac ctgacgcacg ccagacgcta
    25741 aataacttgt cacaggcgtt aggctggctc ttgccggtta gccacagctc tttcgcccct
    25801 tgccaggctt cttcactctg cccggtgttc catttcgcat agtagtaatt acattgcgct
    25861 tcggtagttc cgggcttttc cgggctaaag gctaacaagc cacgccagtc ttcacgccgc
    25921 gccagttcat tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg
    25981 cgaacaaagt tagtgaccgt caccgccggt tgattcatca ggtcatcggt gatctggcgg
    26041 tattccaggt agggataaag cggataatcc ttcagtccag gcatcatttg ttcgaccaca
    26101 tccatctgtc gattatccca ggcctgcttg atttgcgcgt aacggctacg ctgctcatcc
    26161 agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac agacaccggc agccaacagc
    26221 cgccaggtaa cttgtttggc tttttccaca agcgcatcct ctaagtgtaa atacatcaat
    26281 gcagcgtcat accgcgtaat agatttatgc taaccagtca ttgccgttta cgccacgtta
    26341 cggacacttt tttactttta ctgcgaaggt gatcgaccaa ctgggattag gcggggaaac
    26401 aggctacact tcgcctttga aaactcatca tcacgataaa catagaggcg aagtccaacg
    26461 tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata
    26521 ttttgaaaaa catctctctg agtttcttcc ctggggcaaa aattggtgtc ctgggtctga
    26581 acggcgcggg taagtccacc ctgctgcgca ttatggcggg cattgataaa gacatcgaag
    26641 gtgaagcgcg tccgcagcca gacatcaaga ttggttatct gccgcaggaa ccgcagctga
    26701 acccggaaca caccgtgcgt gagtccattg aagaagcggt ttcagaagtg gttaacgccc
    26761 tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat tttgacaagc
    26821 tggccgctga acaaggccgt ctggaagaga tcattcaggc tcacgacggt cataacctga
    26881 acgtacagct ggagcgtgcg gcggatgcgc tgcgtctgcc agattgggac gcgaaaatcg
    26941 ctaacctctc cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctg ctggaaaaac
    27001 cagacatgct gctgctcgac gaaccgacca accacctgga tgccgaatcc gtggcgtggc
    27061 tggaacgctt cctgcacgac ttcgaaggca ccgtggtggc gattactcac gaccgttact
    27121 tcctcgataa cgttgcaggc tggatcctcg aactggaccg cggtgaaggt attccgtggg
    27181 aaggtaacta ctcctcctgg ctggatcaga aagatcagcg tctggcgcag gaagcttcac
    27241 aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt cagggaacta
    27301 aaggcagcca gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg aacagcaccg
    27361 aataccagaa acgtaacgaa accaacgaac tgtttattcc acctggaccg cgtctgggcg
    27421 ataaagtgct ggaagtcagc aacctgcgta aatcctacgg cgatcgcctg ctgattgatg
    27481 acctgagctt ctcgatcccg aaaggggcga tcgtcgggat catcggtccg aacggcgcgg
    27541 gtaaatcgac cctgttccgt atgatctccg gtcaggaaca gccggacagc ggcaccatca
    27601 ctttgggtga aacggtgaaa ctggcatcgg ttgatcagtt ccgtgactca atggataaca
    27661 gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat tatgaagatc ggcaacaccg
    27721 agatgccaag ccgcgcctac gttggccgct ttaactttaa aggggttgat cagggtaaac
    27781 gtgttggcga actttccggt ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg
    27841 ttggcggcaa catgctgctg ctcgacgaac caaccaacga cctggatatc gaaaccctgc
    27901 gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc gatggttatc tcgcacgacc
    27961 gttggttcct cgaccgtatc gccacgcaca tcctggacta ccaggatgaa ggtaaagttg
    28021 agttcttcga aggtaacttt actgagtacg aagagtacaa aaaacgcacg ctgggcgcag
    28081 acgcgctgga gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc
    28141 ggatgcggcg cgaacgcctt atccggccta caaaaccagc aatttcaata cgttgtaaaa
    28201 ctgtaggcct gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat
    28261 gcggcgtgaa caccttttcc ggtctacaac agccttagca tcctcaattt cgtggcgggt
    28321 cacccgtgca ggttttttca aaccttgttt aatctcgaca gcctcttcca gagaggtttt
    28381 caactcatca aaaaaactca ttttatacct cgcctttgag cagtttcgtc acggttatcc
    28441 ttgctccccc atcatctccc gcaccagctc cacgcagcgc aggaaacggc tgtcgtaatc
    28501 gtcctcttca acccgcacga attcgatatt gttctcttcc agcatctcca ccagcaagtt
    28561 ctggaactct ttgcgatcca ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg
    28621 cgtgttgttc tccagcagga tcaccagatc gaaacggtat tcatcaatca gcgcctgcac
    28681 gaacggatgc tcacgccctt cgtacttttt gcagaacgcc tgggtagtga caaaatcggt
    28741 gtcaataaac gccactttat tggcgtattt cactgcaaaa tcaatgtatt gtgcgtggcc
    28801 caacgcgatt ttatcgtagt cggaatactg caatgcgatc tcatcaccgc cgaggtgtga
    28861 aaagacataa tcgcggccat attcccacgc actggtggtg ttgaagatat tggcaagttt
    28921 gtttaccagg gtggatttac cgctcgactc gccgccaagg atcgccacgg tacgcacaaa
    28981 aaacggcttc acttcggtag gaatatattc ccagtagcgg aacgggtttt cgcggatctg
    29041 cgcaccgctg atactcataa aggtacgttt cggatcaacc agcaccgtct cgatccccag
    29101 atgttccata tactgtggcg catcggcttc ttccgaggtg tagatcaggt ccggctggat
    29161 ccccttttca gccataaact ttttgatgcc gttgctccac acatcccagc catgcggata
    29221 cggctccatg ccctcttcgt tgaaagcatg aatacggata tttttctgat atttaaaggt
    29281 ttgcaatagc cagcgcagac gatccggcac cgtgggctgc tgcgacatgg cgctgtcttc
    29341 aaacaacgcg cggtcgcggg tatcgtcaaa acccataatg atatgcagct cgtcaacctg
    29401 gctacaggcg cgctggataa ggtagatatg tccggtatgc agcgggtaga acttaccgaa
    29461 tacgacacca atggttttct tctgccgggg aaattcaagc cccaaaaaac ggtgcaatgc
    29521 ctccagcttt tgcgcgctgg ggcttttaat tttggcattc agtaactggc ttaaataccc
    29581 tttggtcata ccgctggcat cagctacctg ctgtagcgtg cagccctgtt gcttgatggc
    29641 agttttcagg taatcaaatg acgacatatc tccctccgta tctctcatta taagtcgtcg
    29701 aacacgctaa gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc ttccggcgct
    29761 tttttcggta cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt cgccgcttca
    29821 gaaatccgct cctggccact gggcaccggg cggatctccc ctgccagccc gacttcacca
    29881 aacaccacca gatcctgtgg cagcggtctg tcacgcaggc tggaaaccat cgccagcagt
    29941 aacgctaagt cggcgctggt ttcggttacc ttcacgccgc cgaccacgtt cacaaacaca
    30001 tcctgatcgg ccatttgcag accaccgtga cggtgcaaga cagccagcag gattgccaga
    30061 cggttttgtt ccagccccac tgccacgcgg cgcggattcg ccattatcga gtgatcgacc
    30121 agcgcctgaa tctccaccag caacggacgc gttccttccc acaccaccat cacggagctg
    30181 ccggaagtca cttcatctcc gcgacttaag aaaattgccg aagggttgct gacttcacgc
    30241 agcccctgtt cggtcatcgc gaagacgccc agctcattca ccgcgccgaa gcggttttta
    30301 tggctgcgta aggtgcgaaa acgggagtcg gcatcgccat ccagaagcac cgaacagtcg
    30361 atacagtgtt ccagcacttt cgggccagcc agcgaaccat ctttggttac gtgtccaacc
    30421 atgacaatcg ccacaccgcg cgttttggcg aaacgcgtca gataagccgc cgtttcacgc
    30481 acctgcgcca cgctaccagg cgatgattgt acatccgcca tatgcatcac ctggatcgag
    30541 tcaattacca tcagcttcgg ttgctcttct tcggcaatca ggcagatctg ctcgatgctg
    30601 gtttccgaca acatattgag attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc
    30661 acctgttgca gcgactcttc gccggtgaca tacagcgttt tcatctgctg ggccagtttg
    30721 cacagcgttt gcagcaacag cgtggatttc cccgcaccag ggttaccgcc aatcagaatg
    30781 gcacttcctg gcaccacgcc gccgcctagt acgcggtcga actctttaaa tccggtggaa
    30841 aaacgcggca gctcttcaag gctgatatcg gagagtttct ggacttttgc caccccggcg
    30901 ctaccggcat agccgctgag acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc
    30961 acctcggtga tggtgttcca ggcatgacag gcactgcact gcccctgcca gcgcggataa
    31021 tcggccccgc attcattaca aacaaaggcg cgttttggag cttttgccac ggtgtacctc
    31081 gttaatgctg tgccgcccgc aggatgacgg gcaggcaatt acttctgatt caggctgcca
    31141 gagaggatgc agaatacccc catcaggtca gcgtgacgga tggtgacttc cgccttttca
    31201 ttcacttttg gcttggcatg gtaggcaatc cccagccctg ctgctttgat catcggcagg
    31261 tcattagctc catcgccaat cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg
    31321 aggcgagtca gagttttcgc tttgtactgt gcgtctacga tgtcgccgat cacattgccg
    31381 gtaaatttac cgtccatgat ctccagttca ttggctagca cggctgtcag gcgcagcttg
    31441 tcgcgcaggt attcagcaaa gaaagtaaag ccgccggagg caatcgccac tttccagccc
    31501 agcgtttcca gcttgagcac cagctgcgtt aagcctggca tcagcggcag attttcacgc
    31561 acctgttgca gaatattggc gtcagcgcct ttcagcgtcg ccacgcggct gcgcaggctg
    31621 gcggtaaaat cgagttcgcc gcgcatcgcc cgttcggtta cctccgctac catctcgccc
    31681 gttccggcca gcttggcaat ttcatcaata cattcaatct ggatggcggt ggaatccatg
    31741 tccatcacca gcaaacccgg cgtgcgcagg tgcgggattt tccccagcgg ggcgacatcc
    31801 agctgcgctt cgtgggccag gcgtgtagcc cgtgcggtga gtgaacctgc cagacgaatc
    31861 acctgataat cttccacgca ccaggcggca acaatcacca tcgccgcacc cagtttgctc
    31921 tggtattggg tcagacgctg tttatccagc ccacgaccat acagcagcca gccgctacga
    31981 gctgcgtggt aatccagtgg catcacttca tcaccactta atgaaagagg cagacccggc
    32041 cataaagaga catcttcagg caggtcgcac caggtaatgt taggcattaa ggctcctgta
    32101 aaatcgttcg aagcagggaa aataacgcat gaggctacct tgtatccatt gcttctggca
    32161 acattaagtc tcaaattttc aaagggtgga agatggctcg cacaaaactg aaattccggc
    32221 tacatcgggc agtgattgtc ctgttctgtc ttgccttgtt agtggcactg atgcagggag
    32281 cgtcatggtt tagtcaaaac caccagcgac agcgtaatcc acagctggaa gaactggccc
    32341 gcaccctggc gcgtcaggtg acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg
    32401 atgaaaaacg cattcaggcg atcctcgatc agttaacgga tgaaagccgt atcctcgacg
    32461 cgggtgtgta tgacgaacaa ggcgatctta tcgcacgttc tggcgaaagc gtcgaagtgc
    32521 gcgatcggct ggcgctcgac ggtaaaaaag caggcggcta ttttaaccag cagattgtcg
    32581 agccaattgc gggtaaaaac ggaccgctcg gctatctgcg cctgacactc gacacccata
    32641 cgctcgccac cgaagcccaa caggtggata acaccactaa cattttacgc ctgatgttgc
    32701 tgctctcact ggcaatcggc gtagtgctga cccgcacgca gctacagggt aaacgcaccc
    32761 gctggcagca atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa
    32821 gcgagaaaaa agagtgaccc attactacaa gaaaggaaat cgttatgtcc acattacgcc
    32881 tgctcatctc tgactcttac gacccgtggt ttaacctggc ggtggaagag tgtatttttc
    32941 gccaaatgcc cgccacgcag cgcgttctgt ttctctggcg caatgccgac acggtagtaa
    33001 ttggtcgcgc gcagaacccg tggaaagagt gtaatacccg gcggatggaa gaagataacg
    33061 tccgcctggc gcggcgcagt agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct
    33121 gctttacctt tatggctggc aagccggagt acgataaaac tatctccacg tcgattgtgc
    33181 tcaatgcgct gaacgcgctc ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg
    33241 tgaaaaccgt cgaaggcgac cgcaaagtct caggctcggc ctatcgcgaa accaaagatc
    33301 gcggcttcca ccacggcacc ttgctactca atgccgacct cagccgcctg gcaaactatc
    33361 tcaatccgga taaaaagaaa ctggcggcga aaggcattac ttcagtgcgt tcccgcgtga
    33421 ccaacctcac cgagctgctg ccggggctca cccatgagca ggtttgcgag gccataaccg
    33481 aggccttttt cgcccattat ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa
    33541 cgccagactt gccaaacttc gccgaaacct ttgcccgtca gagtagctga gagtggaact
    33601 tcggtcaggc tccggcattc tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg
    33661 aactgcattt cgacgttgaa aaaggccata tcacccgcgc ccaggtattt accgacagcc
    33721 tcaaccccgc gccgctggaa gccctcgccg ggcgactgca aggctgcctg taccgcgcgg
    33781 atatgctgca acaagagtgc gaagcgctgt tggttgactt cccggaccag gaaaaagagc
    33841 tacgggagtt gtcgacgtgg atagcggggg cggtaaggta actcgctgat taaaatatcc
    33901 aaggaaggaa atatgatcga tctggaaaac caggaacggg agattatcaa tttaatgttc
    33961 agccagcgta tttcctggct agccgccgtg cgtatcaggc ataaattatc gcttgctgaa
    34021 gtaagtaaaa tgttaggtat ctcgattaat tctctaaaac agatcgaaaa gacggaacgg
    34081 cttagctcaa acatcaaaag caaaatggca gaaatttatg gttgtccacc agagttgctg
    34141 atatgccctt cctggatgac ggctgaacat aaatgaatag attcgtgaat agatacttga
    34201 atgtatttta ttttctatcc atatgaaaaa taatagtaaa tctataaatc agttgctatt
    34261 gatgaataga tactaaaaag ccggagcagt cccccactcc ggcttaacac gacaatcgcc
    34321 tttcagcgaa acacaattac tctttatcgc ccagcagaac ggattccagt gcgattttga
    34381 tcatgtcgtt gaaggtggtc tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt
    34441 ggtcagacac ggtgcagatg gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga
    34501 taccagccgc ttccatttcc acgccgagga tgccgtattt ttccatcacg tcgaacattt
    34561 cgccgtccgg agagtagaac aggtcagcgg agaacaggtt acccacgcga gcatcaacgc
    34621 ccagtgcttt agctgcatct actgcgttac gcaccatgtc aaagtcagcg atagcggcaa
    34681 agtcatggtc tttaaaacgg atgcggttaa ctttggaatc ggtgcaggcc cccataccga
    34741 taacgacgtc gcgcagtttt acgtgcggca gaactgcgcc acaggaacca acgcggataa
    34801 ttttcttcac gccgaaatcg gtgatcagtt ctttggtgta gatggagcag gacgggatac
    34861 ccataccgtg acccattacg gaaattttgc ggcctttgta agtaccagtg aagcccagca
    34921 taccgcgaac gttgttcact tcacgggcat cttcaaggaa agtttcagca atatacttcg
    34981 cacgcagcgg gtcgcctggc atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa
    35041 tgtgtggggt agccattgtt ttatcctttt tgtgacataa caaaggcgga gtcaacgctc
    35101 cgcccaaatc catcagaaca tggctttgcc atattccata tcagaagtac caaaatattt
    35161 tgccagagtc tggccgatat ccgcgaaggt ttcacggtga cccagtgagc ccggttttac
    35221 tttcgggcca tataccagta ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt
    35281 cggatcgcaa ccgtggtcag cggtgaggat caggatgtcg tcatcgcgca gcagagacat
    35341 cagctccggc agacggcggt cgaacagttc cagacccgca gcataaccgg cgacgtcgcg
    35401 acggtggccc caggaagagt cgaagtcaac gaagttggtg aagacgatgg tgttatcacc
    35461 cgcttctttc atctctttga tggtggcgtc aaacagcgcg tccaagccag tcgctttcac
    35521 ttttttggtg ataccgcagt tggcgtagat gtctgcaatt ttaccgacag aaaccacctg
    35581 gccgtgtttt tcatcaacca gtttctgcag cacggtcggt gctggcggct caacagccag
    35641 gtcgtgacgg ttaccggtac gctggaagtt accagctttg tcaccgataa acggacgagc
    35701 gataacacga ccgatattgt agccgccgtt ggtcagctct tcacgggcga tttcgcacag
    35761 ttcgtagagt ttatccagac cgaaagtttc ttcatggcag gcaatctgga acacggagtc
    35821 agcggaggta tagaaaatcg gcttgccggt tttcatgtgc tcttcgccca gttgatccag
    35881 aatgaccgta ccggaagagt ggcagttacc gaggtaaccc ggcagattag cgcgttcgac
    35941 cagtttatcc agcagctctt gcgggaagct gttttcgtga tcggagaaat atccccactc
    36001 aaacagaacc gggacaccgg cgatttccca gtgaccagac ggggtatctt taccggatga
    36061 catttcgtgc gcccatgcgt acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg
    36121 aatgaaaccg gtagaacctt cgtgtgcttt cgccagcccc agacggatca gatttggcag
    36181 attgagcggc cctttacgac cattatcagc ttcgcctttg gcacaagctt ctgcgatatg
    36241 acccagggtg tcagccccga cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc
    36301 gaatgagtcc agcaccataa taaatgcacg tttcatatgt tctccgtact cagtgcttca
    36361 aatttaaatg atcagatcag tataccgtta ttcgctgata cggcgataga cagttggtgt
    36421 gctttccggt gcgttatcgg caagtttaat tgccgctttc accgctttcg ccgcttcctg
    36481 ccagctgttt tcgtctttcg cgaggataac cgccagcgga cgctgaccgt ctacatggtc
    36541 gcccagacgc gccatatcag taaagccgac gctgtaatcg atggtgtcag atgcctgacg
    36601 acgaccgccg cccattgcaa ccactgccat ccccagcgcg cgggtatcca tttcactgac
    36661 aaaaccttcg gtatcagcat agactgcttt cgtcagcatc gctgtcggca gatacttcgc
    36721 gtagttctca acgaagtcgg tcgggccttt ttgtgccgct accatacgac caaagacttc
    36781 tgccgcttta ccgttgtcca gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt
    36841 cgccagtttg ccggagatca gcatctccac gcacagcacc atcgtgacat caaacagacg
    36901 cgggttacga tactcacccg tcaggaactg caccgcttca cgaacttcaa ccgcgttacc
    36961 tgcactggag gccagtacct gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc
    37021 gccgttagcc acgccaacaa tcgcttcggc aagggcttca gagagttcgt aggtcggcat
    37081 aaacgcgccg ctacccactt tcacgtccat caccagcgcg tccagacctt ccgccagttt
    37141 cttcgccagg atcgaggcag tgatcagcgg gatggagtcc acggttgcgg taatatcacg
    37201 ggtcgcgtag aaacgtttat cagccggagc cagtgaactg gtctgaccga taatcgccac
    37261 gccgacgtct ttaataattt cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg
    37321 gatggattcc agtttgtcga gcgtaccgcc agtatgaccg aggccgcgac cggagatcat
    37381 cggaatatag ccgccgcagg ctgcgaccat cggccccaac atcagcgaag tgacatcgcc
    37441 gacgccgccg gtcgagtgtt tatcaacaat cgggccattc agatgcaggc ttttccagtc
    37501 gagaacggtt cctgaatctc gcatcgccat ggtcagcgag acacgctcag gcattgtcat
    37561 atcgtggaag aaaatggtca tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc
    37621 gcgaataccg ttgataaaga aacgaatttc ttcatcgctc agcgcatgac catcacgttt
    37681 tttacgaata atttcttgtg cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc
    37741 ggcttttgcc ggatgcgtct acgccttatc cggcctacgg gtagatacgt ctcgtaggcc
    37801 tgataagcgc agcgcatcag gcgtaaagca tcttacttag tagctgctgg cgctcttacc
    37861 gtcgccgtgg cccagcgctt tcagcaggct tgccagcagg ctggaagcac caaagcggta
    37921 gtgacgcgca tcagcccagt cagcaccgaa cagttcatct gcgatggcga gatatttctg
    37981 cgcatcttcc gcagtacgca cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc
    38041 catataacgg atcacttcca tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac
    38101 tttaccggta gaggttttga tgaagtccgc acccgctttg atggagattt cagacgcttt
    38161 acggatcagc gcttcgtctt tcagttcgcc ggtttcgatg atcactttca gcagtacatt
    38221 cgctgccgcg caagcctctt tacaggcttt caccaggtca aaaccaacct gctcgtcacc
    38281 cgccatcagc gcgcggtacg ggaacaccac gtcaacttca tcagcaccgt aggcgattgc
    38341 cgcacgggtt tctgccagcg cgatttcgat gtcgtcgtta ccgtgtggga agttggttac
    38401 cgtagcgata cggatttccg gggtgccctg ctctttcaga gttttgcgag caatcgggat
    38461 aaaacgagga tagatacaga tagcggcggt attgccgacc ggagttttgg cctgatgaca
    38521 cagagcaatt actttctcgt cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt
    38581 cagtgcacgc aggctgcttg ctttcagatc agtcatttca ttctcctggc ttgtcgccaa
    38641 taaaattcac cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca cacttcgata
    38701 cacatcacaa ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg atctggttca
    38761 aataattcac tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac
    38821 atcacgaaac ttcttacaat ggcgcatcac cttcggcata aaaggaaaag aagatgccca
    38881 cttctcatga aaatgcactg caacaacgtt gccagcaaat tgtcaccagc ccagtgctta
    38941 gcccggagca gaagcgccat tttctggcac tggaagcaga aaacaatctg ccttacccac
    39001 agcttcctgc cgaagcccgc cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag
    39061 gtcatgcgcc gtacaaaccg cgctatgtct tacccgatta cgctcgtttt ctggcgaacg
    39121 gttccgaatg gctggagctg gaaggcgcga aagatcttga tgacgcactc tctctgctga
    39181 ccattcttta ccaccacgta ccgtcggtca catcgatgcc ggtctacctg gggcaactgg
    39241 atgcgttgtt gcaaccgtat gttagaattc taacacaaaa cgagatcgat gttcgaataa
    39301 aacgtttctg gcgttacctc gacagaaccc tgccagatgc ctttatgcac gccaatatcg
    39361 gcccgtctga ttcgcccatt acccgcgcga tcttacgtgc agacgcgcag gtttcaccga
    39421 acctgacctt tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga
    39481 agaacatctg tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt
    39541 tcacaaaagg gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg
    39601 gcagcacgct ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg
    39661 acttctttac gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc
    39721 ggtgtgaatt cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag
    39781 ggctgattaa ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg
    39841 ttaacttgct gtgtgagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa
    39901 atgaagtggg ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga
    39961 aatatggctg gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatatcg
    40021 gcaccacgcc gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc
    40081 aaactgtcgc cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg
    40141 acgaaaccat caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag
    40201 ccggaatgcg tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt
    40261 atatggtgcg tttgtcggat ttagaaaaat atcgcgccga aggttcacgc accaacacca
    40321 cctggctggg cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga
    40381 taagccatga acagcagatg cgctttagtc tgtaagatta tccccttctc ctgcgttgac
    40441 gggccaggca gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt
    40501 cacaatccgt ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg
    40561 catcaggcgt tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa
    40621 tgtgatacct gcctgaagat gtgtccgcaa catgccacac caatggcgca atccatgagc
    40681 gtggacgaag tgcttagcca tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta
    40741 agcggcggcg aagtcacgac ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa
    40801 aacgatccgc aactgcgcca tctcacctgt ctggtggaca gtaacggtat gttgagcgaa
    40861 accggctggg aaaaattact cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg
    40921 ggtagcgaat gtcatcaaca attaaccgga cgcgataatc aacagattaa gcgcagcatc
    40981 tatttgctgg cagagcgcgg caagctggcg gaactgcgtt tgctgatgat cccaggccag
    41041 gtggattatt tgcaacacat cgaagaactg gcggcgttta tcaagggact tggagatgtt
    41101 ccggttcgcc tgaacgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg
    41161 agcgccacgc cggaagacgt tgagccgttg gctgatgcat taaaggtgcg cggggtgagc
    41221 cggttgatat ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca
    41281 gacttgtagg cctgataaga cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg
    41341 gatgcggcgt gaacgcctta tccggcctac ggttcggcac aaacctgtag gcctgataag
    41401 acgcgttaag cgtcgcatca ggcattatgc gccgactgcc ggatgctgcc tacgaagggc
    41461 aaactaccca cgaacgttaa acaccgcata cgtgttatta agcaacactt cggcaatctc
    41521 atccgcgggt tccgggcgta actcgcaaag cacggcaaac acacgggcag cctgttccgg
    41581 gcggttaggc tgcccctgaa aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa
    41641 caacgatgcc agcggtaatt ttgcgatgac atcgcgggtt ttactggcgc gtggataggt
    41701 gatagtaccg cctacgccga ttttgtagcc cagctgtaca aaccgttcag cctgttgctg
    41761 gctgccagaa aatccgtgga caacaccagc acgcggcaaa tcgtggcgtt taagatgcat
    41821 cgccagtttg tcgtgcgtgc gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc
    41881 cagtttcagt tgttcgtcga gtaaccactg ctgcctctca aattgcggat cgtcgccgaa
    41941 gagatccaga ccgatctccc ccaccgccac caccttcgcc ggacgccttt ccagcgcctg
    42001 ctgtagctgc tcaagagaca catcgctatg tttttccaac ataccaggat gcaagcccaa
    42061 tgcggcatac agcggttgat aattttccgc taacgcctgc acgcgggcaa aattttccgc
    42121 ctctgtcgcc ggaacaatga tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc
    42181 ctcttcatcg ccaataaaag gcgggaaatc aaaatggcag tgagtgtcga taaaacggca
    42241 aatcaagcca gatcctcatt gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact
    42301 tcggcaacca gcgtatcgtt ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc
    42361 accactggca ccagatggcg ggtaagcggc gctttttcag ttaatagctt gccaaccgtg
    42421 gcgaggaaat aacgaccgca taaacgccca agtttatagt cttcacgcag cgccggaatc
    42481 cgactgccaa gcgcgatact atgtaatggc ttcggcggat aaatttcgga tatccgcagc
    42541 ttgcccggtg gtttctcaat aaattgctga atgtcacgat agctggtttc atgatgctgc
    42601 accagattga ccagcggctg caggctactg tcacccagcc agcgttccat gcgtttgaac
    42661 cactgcggcg tgtagtacat ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg
    42721 ccctgccttg ccgcctcttt aaccggaatc gcatcactga tcccgccatc caggtagtta
    42781 atgccttcca gcgacactcc gctacgataa aagccaggta tcgcactgga ggcgcgaatc
    42841 acatccagcc agttttgttt ggttggtaaa aagtaattcg gcgcgtagtc atcctgacga
    42901 caggcgcaca tataaaacga tttgccgctg tcaaacaacc gcgcggcggt atccatttgc
    42961 aacggcatct ggcttgcggt ggcctccacc agccagtcga gatcgataag atttcctcca
    43021 cggacaaagc gcaatggatc gaaaaattcg cgttttgtgg tatagcgcat gatgactttg
    43081 cgcgcgtaac cgggctgatt gcagataaac gccgagaggt tctgcgcccc ggcagatgtg
    43141 ccgagataaa gatcgaaagg attaaactgc gcgcgcataa actcatccag cacgccagcc
    43201 gtgaaaattc cacgctgtcc gccgccttcg cacaccagag ctattcgtcc aggctggaac
    43261 ggccttagcg acaacggcgc aatattacca agcgttacag gtattcgctg ccccacttct
    43321 gccttcctgt ttttgttgtg aaagaaagac aaagtaacgc aatttattat tcgctaaaac
    43381 cgcaaaagcc agtccgctgg actggctttg gataaaattt tcttaatggt ctgaaatcta
    43441 gggtcgtttt cggcccatga acaaactcac taggaacaga ataatcccga cgacaaagac
    43501 aattttagct gcgcctgcag cggtaccggc cagaccacca aacccaagtg cggcggcgat
    43561 taacgcgata accagaaata tgatgcccca acgaaacata agcgtctcct ttaccatagt
    43621 taatgtcacc gcttgatatg agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc
    43681 acatcgcgcc tcccgacaaa gttcgggagg acgaattacg acgaattact tagttttcag
    43741 atcattttta acgcttttca caccatctac cgctttggcg atactttcag cacggtcact
    43801 ttgtgcctga gaatctacgg taccggagag ctgaaccacg ccatcggtgg tttcaacttt
    43861 cactttacgt gaagggacga tatcgtccgc cagcagtttg gctttgattt cactggtggt
    43921 ggcggtgtca cccgcgtagt ccttcaccga gccttcttta gcgtcgcgaa cgtgcagttt
    43981 gtcgctgaca gaggtcacgc cttcaacgcc tttcgccact ttcactgcct cttcggcctg
    44041 ggcctgactt tcaacgaaac cgctcagggt cacgactttt tgatcggttt tgacagagat
    44101 atcggtgctc ttgatgttgt catgatccac cagcgccgcc ttcactttcg cggtgatggc
    44161 gctgtcatcc atgaaattac cgactttatt catagagcta tcgacttttt gccctgcgct
    44221 ttcattggta gtctgcgcgt tgttttccgc gtaggcagag ccggtcgcga cggcagaggt
    44281 caacattaca gccagcagag ttttcgaaat cttcagtctt gtcatagtca tcgatttatt
    44341 cctgtatgtt tgctcgtaat ttgagcctag caacacgagg ttgcattgct gaatagggag
    44401 agacttcacc ctctacagaa gtcaatggtc gccatcacaa aagcgatggg tgatgaataa
    44461 cgaccattac agtctctgaa tcagttatta atatcggtag aatgacaatc gacggcttta
    44521 gatactgata tctacgcatt gaacggtatt taacgccatc agaaatgtca tcactttgtt
    44581 aaatatagat cacaattttg aaaccgctcg ggatatcagc gaaaacataa gcaaaagtga
    44641 atgttttaag aacattccgt aagcggctaa taaggaaggg aaattgacag ggcgcagcgg
    44701 ctaccgcgcc ctggaggcaa gaattaatgc tcgcgggtct ggtggaactg aacgtccgga
    44761 taacgttcct gtgccaggcg caggttgacc atgctggtag cgatgtaagc gaggttatcg
    44821 ccgccatcaa gcgccagttg gctttcgttc ttacgcttga actcttcgaa tttcttcgcg
    44881 tctgcacatt ctacccagcg ggcagtggca acgttgactg actcatacac tgcttcaacg
    44941 ttgtattcgc tcttcaggcg cgctaccacc acatcaaact gcagcacacc aaccgcacca
    45001 acgatcagat cgttgttgga gattggacgg aacacctgca ccgcgccctc ttcggaaagc
    45061 tgtaccagcc ctttgagcag ctgtttttgc ttcagcggat ctttcaggcg gatacgacgg
    45121 aacagttctg gtgcgaagtt cggaataccg gtgaacttca tcatctcacc ctgggtaaag
    45181 gtgtcgccga tctgaatggt gccgtggttg tgcaggccga ggatatcgcc cggatacgct
    45241 tcttcaacgt gcgaacggtc acccgccata aaggtcagcg cgtcggagat caccacatct
    45301 ttcgcagtgc gcacctggcg cagtttcatg cctttttcat atttaccgga caccacacgc
    45361 ataaacgcca cgcggtcgcg gtgtttcggg tccatgttgg cctgaatttt aaatacgaag
    45421 ccggtaaatt tatcttcgct cgcttctacg gtacgggtat cggtctgacg cggcatcggc
    45481 gcaggcgccc actccaccag gccattcagc atatgatcaa cgccgaagtt acccagcgca
    45541 gtaccgaaga ataccggagt gatttcgccc gcaaggaaca gctctttgtc gaactcgtta
    45601 gacgcgcctt taaccagttc cagttcgtca cgcagctgct gtgccagatc ttcaccaacc
    45661 gcagcatcga gatccgggtt attcagccct ttaacaatgc ggacttcctg aatggtgtgc
    45721 cctttaccgc tctgatagag ataggtttca tctttataaa ggtggtaaac gcctttaaac
    45781 agcttgccgc agccaattgg ccaggtgatc ggtgcgcagc caattttcag ctcgttctca
    45841 acttcatcga gaagttccat cgggtcgcgg atatcacggt caagtttgtt cataaaggtg
    45901 aggatcggcg tgtcgcgcag acgggtaact tccatcagct tacgggtacg atcttcaaca
    45961 ccttttgcgg cgtcgataac catcaggcag cagtccaccg ccgtcagggt acgataggta
    46021 tcttccgaga agtcttcgtg ccccggggtg tcgagcaggt taaccaggca atcgtgatac
    46081 ggaaactgca tcacagacgt agtaatggag atcccacgct gcttttccat ctccatccag
    46141 tccgacttag cgtgctggtt ggaaccacgg ccttttactg taccggcggt ctgaatggcc
    46201 tgtccgaaca gcagcacctt ctcggtgatg gtagttttac cggcgtccgg gtgagaaata
    46261 atggcaaaag tgcggcgctt cgccacctct tgcaaataag gagacaacgt cataattctt
    46321 tcttcttgag taagcggcat cgcgccgcgc ttgttgaaaa cgaaaaattg cggctatttt
    46381 acccatcaac gggggggagg caatcagtgt ttacacagga gctgctccag ttcgtgcaac
    46441 gaagaaacgg tccaggtggg cgcgatgcct tctggttgct cgcgattgtg cgcattcagc
    46501 cagcaggtcg caagcccggc gttgatgcca ccgagaatat cggactcggc agtgtcgcca
    46561 accatcagca cgcgtgaacg gtcaggattg cccgcctgtt ccagcgcata atcgaaaatt
    46621 ttcttattcg gtttggcaac gccaacttct tcggaaatca ccagcaaatc gaagtaatca
    46681 cgcaggcccg tccgttccag acgcacttgc tgcaaggcgc taaaaccgtt ggtgatgatg
    46741 ccgattttag cgttgccacg aatggcgtta agcagagaaa ccgcgcccgg caacggcgta
    46801 cagatttccg ccatcgcatt aataaaggca tcgttaagca agcctgcttc aactttcagc
    46861 cgttcggccc agctctcgaa acgcccgtgc tgaagctgta atgaagtgat cgcgccgttt
    46921 tgataatcca cccacagtgg cttgttaacg gcctgatagt cctgaaaatc ttcagcggta
    46981 aaggtgacgc tgtaatcaag aaacatccgc tgcaggccgg tgaatgagtc aaaggtaaac
    47041 agcgtttcat cggcatcaaa gaaaatccag tcccacttca ttattccacc ttgtcttaca
    47101 tactgattgg caacgccatg atgatggcgt cttcgcgacc gtccgtggtg gggtagtaat
    47161 tgcggcgaat cgtcgcctcg ttaaagccta aactttcgta cagggcaatg gcggcagcat
    47221 ttgaggcacg gacttccagc catagtgtcg ccacgccgcg tttttccagt tcgtcgatca
    47281 gatgttccag cagcgcccta cccagcccct gacgctgata gtcgggatcg accgcaatat
    47341 tgaacaatgt agcttcatcc aacaccactt gcgtaatcgc aaacgccgcc attttgccgt
    47401 tttgcgttaa ctgaaagttg agataacgct cgccctggtt gctggcaaag gttttttcac
    47461 tccacggaaa ggcgtgggcg cgttgttcaa tgtggtaagc cgctggtaaa tcagtcgttt
    47521 cgagggaaga aatcgtgttc atatgtgcaa atttgttgcc ataacgcggc gcgtgccgtt
    47581 gggtttaccc gtaattcggt gagcgccggt gatgccacct gagcgccttc caatgatagc
    47641 ggttcgtcag tacccaaccg ccagctgttg cagcgactgc cttgcggcag catcgcgatt
    47701 ttttctggcg tcagttgcag cacttgatcg gggctgacgg ttaatgcgcg cagaacatcg
    47761 ctcactaaag gatcagtcag ggcgggaaga tcgtttgcca ccatcaccag acggacgtgt
    47821 gccgggctcg caatggcaat ctccccctgc aacgcgccag ggcgacgcag cgaccactgg
    47881 gtaatgccca gttgctgtaa ctgccagtct cgtcgggatg tcatagcgaa tcgctcctgt
    47941 tgtcaggggc gcaaatatag caaattcgtc gataccgcgc caacatatgg ctataatcgc
    48001 cgccagtatc aattgaggag cattccatgt ctgcatttac cccggcaagt gaagtcttgc
    48061 tgcgtcacag tgatgatttc gaacaaagcc gtattctgtt tgccggagac ttacaggatg
    48121 acctgcccgc gcgtttagat accgcggcca gccgtgctca tacccagcaa ttccaccatt
    48181 ggcaggtgtt aagctgccag atgggggata acgcccgttt tagtctggtc gccacggcga
    48241 atgatgtcgc agattgcgat acgctgattt actactggcc gaagaacaaa ccggaagccc
    48301 agttccagtt gatgaattta ctttctctgc tgccagtggg gacagatatt tttgtcgttg
    48361 gcgagaaccg cagcggcgtg cgcagcgctg agcagatgct ggcagattat gcgccgttga
    48421 ataaagtcga cagcgctcgt cgctgtggcc tctattttgg tcgtctggaa aaacagccag
    48481 tatttgatgc ggataaattc tggggcgaat acagcgtcga tggcctgacg gtcaaaacgc
    48541 tgcctggcgt gtttagccgc gacggtctgg atgtcggtag ccagttgctg ctctcgacgt
    48601 taactccgca cacgaaaggt aaagtgctgg atgtcggctg tggcgcgggt gtactttcag
    48661 ttgcctttgc gcgccattcg ccgaaaattc gtctcacctt gtgcgatgtc tctgccccgg
    48721 cggtagaagc cagccgcgca acacttgcag ccaacggtgt tgaaggtgaa gtctttgcca
    48781 gcaacgtctt ttccgaggtg aaaggttgtt ttgatatgat catctccaac ccgccgttcc
    48841 acgatgggat gcaaaccagc ctggatgcgg cgcaaacgct gattcgtggc gcggtgcgtc
    48901 atcttaatag cggcggcgag ctgcgaattg tagcgaacgc cttcctgcct tacccggacg
    48961 tgctggatga gacatttggc ttccatgaag ttatcgcgca aaccgggcgc ttcaaggtat
    49021 atcgcgccat tatgacccgc caggcgaaga aaggttgagt atctgatatc tgtcgccgca
    49081 taccgtgtcg gatgcggcgt gaacgcctta tccgacctac aaaacctgca cgctagtttc
    49141 ctgatggaca tttttccagc aattacacat caggcgataa ttaactattg acgaaaagct
    49201 gaaaaccact agaatgcgcc tccgtggtag caattctttt taagaattga tgg
//
LOCUS       DADLXX010000017        44106 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLXX010000017 DADLXX010000000
VERSION     DADLXX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.017"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1297..2979)
                     /gene="glcA"
                     /locus_tag="JNX27_000951"
     CDS             complement(1297..2979)
                     /gene="glcA"
                     /locus_tag="JNX27_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HAY7882246.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVAFAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3334..5505)
                     /gene="glcB"
                     /locus_tag="JNX27_000952"
     CDS             complement(3334..5505)
                     /gene="glcB"
                     /locus_tag="JNX27_000952"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY7882247.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5527..5931)
                     /locus_tag="JNX27_000953"
     CDS             complement(5527..5931)
                     /locus_tag="JNX27_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY7882248.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5957..7159)
                     /gene="glcF"
                     /locus_tag="JNX27_000954"
     CDS             complement(5957..7159)
                     /gene="glcF"
                     /locus_tag="JNX27_000954"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY7882249.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            complement(7170..8222)
                     /gene="glcE"
                     /locus_tag="JNX27_000955"
     CDS             complement(7170..8222)
                     /gene="glcE"
                     /locus_tag="JNX27_000955"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY7882250.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8222..9721)
                     /gene="glcD"
                     /locus_tag="JNX27_000956"
     CDS             complement(8222..9721)
                     /gene="glcD"
                     /locus_tag="JNX27_000956"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY7882251.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9967..10744
                     /gene="glcC"
                     /locus_tag="JNX27_000957"
                     /pseudo
     CDS             9967..10744
                     /gene="glcC"
                     /locus_tag="JNX27_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10964..12010)
                     /locus_tag="JNX27_000958"
     CDS             complement(10964..12010)
                     /locus_tag="JNX27_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7882252.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKICGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKRMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEM"
     gene            12414..14102
                     /locus_tag="JNX27_000959"
                     /pseudo
     CDS             12414..14102
                     /locus_tag="JNX27_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14117..15013
                     /locus_tag="JNX27_000960"
     CDS             14117..15013
                     /locus_tag="JNX27_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY7882253.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            15046..15294
                     /locus_tag="JNX27_000961"
     CDS             15046..15294
                     /locus_tag="JNX27_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7882254.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15294..16466
                     /locus_tag="JNX27_000962"
     CDS             15294..16466
                     /locus_tag="JNX27_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7882255.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16502..17581)
                     /gene="lptG"
                     /locus_tag="JNX27_000963"
     CDS             complement(16502..17581)
                     /gene="lptG"
                     /locus_tag="JNX27_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7882256.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17578..18648)
                     /gene="lptF"
                     /locus_tag="JNX27_000964"
     CDS             complement(17578..18648)
                     /gene="lptF"
                     /locus_tag="JNX27_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7882257.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18679..19239)
                     /locus_tag="JNX27_000965"
     CDS             complement(18679..19239)
                     /locus_tag="JNX27_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7882258.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19251..20075)
                     /locus_tag="JNX27_000966"
     CDS             complement(19251..20075)
                     /locus_tag="JNX27_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882259.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20075..21424)
                     /locus_tag="JNX27_000967"
     CDS             complement(20075..21424)
                     /locus_tag="JNX27_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY7882260.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNIVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21470..22228)
                     /locus_tag="JNX27_000968"
     CDS             complement(21470..22228)
                     /locus_tag="JNX27_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882261.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22260..22973)
                     /locus_tag="JNX27_000969"
     CDS             complement(22260..22973)
                     /locus_tag="JNX27_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7882262.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23147..23839
                     /locus_tag="JNX27_000970"
     CDS             23147..23839
                     /locus_tag="JNX27_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7882263.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23888..25387)
                     /gene="pitB"
                     /locus_tag="JNX27_000971"
     CDS             complement(23888..25387)
                     /gene="pitB"
                     /locus_tag="JNX27_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY7882264.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25679..27538)
                     /gene="gss"
                     /locus_tag="JNX27_000972"
     CDS             complement(25679..27538)
                     /gene="gss"
                     /locus_tag="JNX27_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7882265.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27743..28609
                     /gene="yghU"
                     /locus_tag="JNX27_000973"
     CDS             27743..28609
                     /gene="yghU"
                     /locus_tag="JNX27_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7882266.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28732..28980)
                     /gene="hybG"
                     /locus_tag="JNX27_000974"
     CDS             complement(28732..28980)
                     /gene="hybG"
                     /locus_tag="JNX27_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7882267.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29114..29455)
                     /gene="hypA"
                     /locus_tag="JNX27_000975"
     CDS             complement(29114..29455)
                     /gene="hypA"
                     /locus_tag="JNX27_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7882268.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29448..29936)
                     /gene="hybE"
                     /locus_tag="JNX27_000976"
     CDS             complement(29448..29936)
                     /gene="hybE"
                     /locus_tag="JNX27_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7882269.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29929..30423)
                     /locus_tag="JNX27_000977"
     CDS             complement(29929..30423)
                     /locus_tag="JNX27_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7882270.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30423..32126)
                     /gene="hybC"
                     /locus_tag="JNX27_000978"
     CDS             complement(30423..32126)
                     /gene="hybC"
                     /locus_tag="JNX27_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7882271.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32123..33301)
                     /gene="hybB"
                     /locus_tag="JNX27_000979"
     CDS             complement(32123..33301)
                     /gene="hybB"
                     /locus_tag="JNX27_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7882272.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33291..34277)
                     /gene="hybA"
                     /locus_tag="JNX27_000980"
     CDS             complement(33291..34277)
                     /gene="hybA"
                     /locus_tag="JNX27_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7882273.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVMVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34280..35398)
                     /gene="hybO"
                     /locus_tag="JNX27_000981"
     CDS             complement(34280..35398)
                     /gene="hybO"
                     /locus_tag="JNX27_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7882274.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPYNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35587..35874)
                     /locus_tag="JNX27_000982"
     CDS             complement(35587..35874)
                     /locus_tag="JNX27_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7882275.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35993..36919)
                     /locus_tag="JNX27_000983"
     CDS             complement(35993..36919)
                     /locus_tag="JNX27_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7882276.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            37037..38077
                     /gene="gpr"
                     /locus_tag="JNX27_000984"
     CDS             37037..38077
                     /gene="gpr"
                     /locus_tag="JNX27_000984"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7882277.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38117..38611)
                     /locus_tag="JNX27_000985"
     CDS             complement(38117..38611)
                     /locus_tag="JNX27_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7882278.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38802..39686
                     /gene="yghA"
                     /locus_tag="JNX27_000986"
     CDS             38802..39686
                     /gene="yghA"
                     /locus_tag="JNX27_000986"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7882279.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39958..40383)
                     /gene="exbD"
                     /locus_tag="JNX27_000987"
     CDS             complement(39958..40383)
                     /gene="exbD"
                     /locus_tag="JNX27_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7882280.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40390..41124)
                     /locus_tag="JNX27_000988"
     CDS             complement(40390..41124)
                     /locus_tag="JNX27_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY7882281.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41376..42563
                     /gene="metC"
                     /locus_tag="JNX27_000989"
     CDS             41376..42563
                     /gene="metC"
                     /locus_tag="JNX27_000989"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7882282.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVVRVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42703..43362
                     /locus_tag="JNX27_000990"
     CDS             42703..43362
                     /locus_tag="JNX27_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7882283.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43402..>44106)
                     /locus_tag="JNX27_000991"
     CDS             complement(43402..>44106)
                     /locus_tag="JNX27_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7882284.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10942 a        11762 c        10842 g        10560 t
ORIGIN      
        1 acgtaccgtt taaacaaaga caatacgtct ctttatttct cataaaaccc caatccctta
       61 cagggcttat tgaaaaattt aaaaatcaca aatttctcaa tactcgaaat gagatcacat
      121 aagaaatcaa aaagccttct catctatttt aagttttccc aacggaaaat aagagctttc
      181 attaatactg aaccacttta ctgttctatt tgttcaggta ttgcattacg gtgtgctgag
      241 atccatcggt gcatccctgt atcccgcgat gagactaaaa tagtcgtgat gtaagcttgt
      301 atcagattct tagtgaatac cacatttgtt ataattaagt gaaaagatac attgctgatt
      361 tacattattt ggtttacaaa atgtcatcac ttgcagagtt atcgctgaca gataccttta
      421 atctcttact actttcgtgt tattgcaata caaccatccg taaagcaggg ctatttttag
      481 caggaaatac tcaggtgtca acattcgaac tccaactctg aaatattcat ataaattaat
      541 gattttttca tttcttcgcg cctaataaaa gtattcaaat tcagatgatt tttctgctca
      601 acagacacaa acaggcaata atactctcca aagcgaacaa tatttggcat tacgatttaa
      661 tgcttcataa tcaccaaatt atttaaccat aaattttctt atttctaaca aaatgattgg
      721 tgtgttgttg gagttaaaat acatacataa tattaacatc tgccaacatt tcactttccg
      781 caagacggat gatttcgaat cctcatccac gttaccctat cccaaatatc tttattaata
      841 ggcagtaacg aacactgtta tagcaagatt aacttattaa cctaacggca tcttttatgt
      901 aacaagtgga gataaattgc ttaaaattga tcctcgacaa atcttaaaag ttctcattat
      961 tctgcaaaat cagctttgcg aataatctta tgtttttggg agttttggga attttgatct
     1021 actcattgtt ctagtgaaaa atataaataa gagaaaaaac gccattcgtc atgaaagcaa
     1081 cagatgaagc aatgtaataa catactaaaa ttgatctcat gaaagtgggt actttataac
     1141 cagcacagaa gccaagttgt ataaatatta aacaattcgc atcgtgaaac ggagactacg
     1201 tataaagaag ccaatcgtaa aaacggttcc ctggcgagat aaacgacaga caaatggaaa
     1261 gccgggtgat atttaccttc atccggcttt attgatttac gagactaaca tcccggtaaa
     1321 cacatacgcc tgcagcaggg tgataacgcc gataacgctg gcaaaaatca gactgtgctt
     1381 cacggtgtag cggaacagtt cagattctcg gcccaccatg cccgtcgcgg cgcaggccac
     1441 ggcgatagat tgcggggaga tcatcttgcc agttacgccg ccgctggtat ttgctgccac
     1501 cagcagagtg tcagagacgt tgatttgctg cgccgtggtc gattgcagtg aaccaaacag
     1561 ggcgtttgag gaagtgtccg acccggtaag gaatacgccc agccagccga gaaacggtga
     1621 gaaaaacggg aacatcacgc ctgtacctgc cagtaccagc gccagcgtgg tggacatgcc
     1681 ggaatagttg gtgacgaagg cgaatgccag caccatgcca atcgacaata tcggccactt
     1741 cagactaatt agcgtttcgg caaagacgcc aatccctttc ttgatgccca cgccgaggat
     1801 gaagatagag ataatcgccg caataaaaat agcggtgccg ccagccgaga gaggttcgaa
     1861 tttaaacacc gcatccattg gcgttggttg ggcgacaatg ggtgccgctt tcaacacttg
     1921 ttgatgcaaa tgagggatct ggaaattaat caccagtgaa taaaacgcgc cgcccggagc
     1981 aaataacgct ttaaacggct tcatggtcca gatggtgacc agcaccgtta agattaaaaa
     2041 cggtgaccac gcacgaatga tttgccccag actatattct gaaggcacgg gaccgccaga
     2101 agatggctta tttaccacca tcgcacctgc ggattgcccc atgctgattg ccgtttcggt
     2161 atttttcggc cgccagactt taaggaataa agcgagtgag acgatactca ccagcgccga
     2221 agtaatatcc ggcagttccg gaccaatata gttagaggta aagaactgag tgacagcgaa
     2281 gcttccccca gcaaccagcg ccgctggcca cgtctctttc acccctttcc agccgtccat
     2341 cattgctacc agccagaacg gcacaagaac cgacaggaac ggtaactgac gtcccgccat
     2401 tgcgccaatg tggaacggat cgattcccgt tacctgaccg gcgaccagaa tcggcacgcc
     2461 caacgcacca aacgccaccg gcgcagtatt ggcaatcaga cacagccccg ccgcgtataa
     2521 cggtttgaag cccaggccca ccagcagcgc accggtaatc gccaccggcg caccaaagcc
     2581 agccgctcct tccagcaacg caccaaagga gaaaccaatc agtaacacct gcaaacgctg
     2641 atcgtcggtg atggagataa ccgagctgcg gataatatcg aactgcccgc tggcaacggt
     2701 taatttatac aggaacaccg ccgcgacaat aatccacgca attggccata atccataaat
     2761 aaagccatag cccgcagcag caaatgccat atcaatcggc attttaaagg cgaatatcgc
     2821 aatcaggata gataatataa gggttattgc tccagcgaca tgtcctttca gacgtaatac
     2881 cgcgaatgca acgaagaaga atattatcgg gatcagggcg accagagcgg atagccccag
     2941 tcctcccatc ggcatataca tttgggtcca ggtaaccata ttgttcagtc tcttattatc
     3001 tttatatgct tgatatactt aaggttgtaa taagcaaaag aggactgaac tgtaaaatat
     3061 aggcgttata ctttacagca acagtacgcc gctaacgcaa ttgctacctc tggcataaca
     3121 agtatatcgg gtaagggttt ctgttccgca cacgcaggcg cagagtatcg ttaagatgtc
     3181 catattgttg ttttaggccc gctagtaatg cgctacgggt atttaatatt gttaaaccct
     3241 gataatcgct ccggttattt ccgggataaa tgtactaccg cagttactat catagccccg
     3301 acaataaaac ttgccggggc ttttttgacg ctattaatga ctttcttttt cgcgtaaacg
     3361 ccaggcgtgt aataacggtt cggtataacc gtttggctgt ttcacgccga ggaagattaa
     3421 atcgctggca gctttaaaag cacacgagtt agcgaaattc cccgccatcg gacgataagc
     3481 cggatcgcca gcattttgct gatcaaccac tttcgccata ttctccagcg acgcctgcac
     3541 ctgttctttg gtcagaatac cgtgacgtaa ccagttggcg atatgctggc tggagatacg
     3601 cagcgttgcg cggtcttcca tcagcgccac gttgtgaata tccggcactt ttgaacaacc
     3661 aatcccctgc tccacccaac gcaccacgta ccccagaatc ccctgcacgt tgttatccag
     3721 ctcttgttgg atctcttgcg ccgaccagtt agcgttttca gccaccggaa tagtcagcag
     3781 atcgtccagc agcggttcaa attcagcatt gaactcggtc tgggcaatgt tggcttgcac
     3841 gctctgtacg ttggtttggt ggtagtgcag cgcatggagc gtagcagcgg ttggtgacgg
     3901 aacccaggct gtgtttgccc cggcacgcag ttggtcgccc ttctggctgt acatgtctgc
     3961 catcaggtcc ggcattgccc acatgccttt accgatttgc gctttaccgc gcagcccaca
     4021 gaacagaccg gaaagcacgt tattacgttc gtaggctttg atccacggcg tcgatttcat
     4081 ctggttttta cgcagcatcg ggccagcttc catcaccgaa tgcatttcat cgccggtacg
     4141 gtcgaggaaa ccggtattga tgaacgccac gcggttgcgc gcctgagcga tacagctccg
     4201 caagttcagc gaggtccggc gttcttcatc cataatgccc attttcaggg tattcggtac
     4261 cataccgagc attgtctcaa tgcgggtaaa cagtttgttg gcgaacgcca cttcctgcgg
     4321 accgtgcatt ttcggtttca caatatagac gctgccagtg cgcgagtttt tctgcacttt
     4381 taaatcatag agggcaatcg cgccagtcat gacgccatca agaatacctt ccgggatttc
     4441 attgccttcg ctgtcccaaa tcacaggaat ggtcatcaaa tgacccacgt tgcggataaa
     4501 cagcaacgag cgtccgtgca gagaaatttc agagccatcg gcggcggtgt actgacgatc
     4561 gtcattcagt ttacgtacga tttgccgacc gtttttctcc attttctctt gcagagtccc
     4621 ctgcatcagg cccagcagat tgcggtacag caggatttta tcttccgcat caaccgccgc
     4681 gaccgaatct tcgcagtcga gaatggtact gatagcagct tcgacgataa catcgttgat
     4741 gtgcgccgga tcgtctttgc caatccgccc gttggcatca atttgcagtt caatatgcag
     4801 gccgttattt ttcagcaaaa tgcaggtcgg cgcagcggca tcgccacggt aaccgacaaa
     4861 ctgtgctgga gtacgtaacg tggtttcttt accatttttc aactggatgc gtaattgttt
     4921 atcgaccacc ttaaacgcca ccacatcctg atagctgccg ttttccagcg gtagtgattc
     4981 atcgaggaaa cgtcgaaccc aggcgataac ctgctcaccg cgctgcggat cgtagccact
     5041 gaccatcgcc ccttcctgcg ggatgatgtc gctgccgtat aacgcatcgt acagtgagcc
     5101 ccagcgagcg ttcgccgcgt tcagcgcgta gcgggcgttc attgccggaa ccaccagctg
     5161 tggccccgcc tgactggtga tttcgctgtc aatgcccgtg gtttccaccg tcacgcgctc
     5221 cggttgcggc accaggtagc ccagttcaca aaggaaagat ttataggccg ctttatcttt
     5281 taccggcccc ggattgctgc gatgccactc atcaagcgcc gcctgaatgc gatcgcgttc
     5341 tgccagcaac tgacgatttt ctggtgccag atcgtgaacg atctcatcaa cattgtgcca
     5401 gaatgcagca gcgtccagtt ccacgcccgg caaaacttct ccatccacaa aacgtttaaa
     5461 attggcgtca atgcgtaaac ggccctgggt tatggtttga ctcattgttt ctctcctcgt
     5521 tttcgcttat ttcgccaaca ccgctgctgc cgctttcgcg acctgcgcat cctgtgctcc
     5581 ggttaaacca gaaacgccca cggcaccaat aatttgccca tcaacaacaa ccggtacgcc
     5641 gccttccagc gacgtcagta acggcgcagt cacgaacgcg gtacgtccgt tgttcaccat
     5701 ctcttcatag cccttagttt cacgacgccc cagcgcggcg gtacgcgctt tctcctggga
     5761 gatgtaagcc gcaatcggcg cgcaatcgtc catgcgactt aacgccagca gatgaccgcc
     5821 gtcatcggca acagcaatgg aaacagacca gttatttttc tgcgcctctt cctgacctgc
     5881 ggcaataatt gcactcgcca tttgctggct aagaatgact ttagttttca tttttttatt
     5941 ccttttcaag ggcttgttat acaatttcaa tccagtgacg cacagaggta cgaccggcgc
     6001 tcgccagatg cgtctggcaa ccaatgttgg cggtgacgat catttccggt ttgccgcttt
     6061 ccagcgcatt cattttgtta tcccgcagct ggcgtgccag atcgggatgc gttaacgcat
     6121 atgttcccgc tgaaccgcag cacagatggc tgtcgggaac gtccgttaag gtaaatccaa
     6181 gacgaagtaa cacgttttcc acttcgccgt tcagcttttg cgcatgttgt agggtacacg
     6241 ggcagtggaa ggccagtttt ttatcgccgc gaattgccag tttttccagc ggttcctcgc
     6301 gcagaagttc gactaaatcg accgccagtt cactgacctg acgcgcttta tcggcatata
     6361 acgcatcgtt tttcagcatc tgcccatact ctttgacgaa cgcgccgcaa ccgctggcgg
     6421 tttgcaaaat tgcctcggca cctgcttcaa tcgcgggcca ccaggcatca atattattgc
     6481 gcgcccgtgc caaccctttc tcctgcgcat taagatgata gtccaccgcg ccacaacagc
     6541 ctgcttcgtt agctgacatg acgctgatcc ccagacgatc cagcactcgc gcagttgccg
     6601 cgttggtgtt gggcgaaagc gtaggctggg cgcagccttc cagcattaaa acccgacgct
     6661 tatggcgcag cggcggacgc ggtttagctt tcaccgtttc agcaggcagt tttgctctga
     6721 cctgttccgg taaaaacggt cgcagcacca gccctacctg cgtcagcgca cggaagaccg
     6781 ccggacgcgg cactacctgg cgcaatcctt cgcgcagcat tcgctccggc agtgggcgtt
     6841 tcactttctg ctcgacaata tcacgcccga tatccagcaa attgtgatag cgcacaccag
     6901 aaggacaggt ggtttcacaa ttgcggcaag tgaggcagcg atcgagatgc tcctgtgttt
     6961 taagcgtgac ttcgttgcct tccagcacct gtttaatcag atagatgcgc ccgcgcggcc
     7021 cgtccagttc atcgcccaga agctgatagg ttgggcaggt tgcggtacaa aatccgcagt
     7081 gaacacaggc gcgcaggatg ctgtcggctt ccagcgcgcg cgcgttctgc cgcatctctt
     7141 cggttaatcg ggtttgcata gcctgctcct caaagttccg cgtacatgcg accggggtta
     7201 aacacgccgc aagggtcgag ctgctgttta agctgctggt gatagcggaa taaaggagcc
     7261 gatagcgggg caaagccacc atctccggca ctaaagcggg tcgcatgacc gccagcgttg
     7321 cgggcgatgc gatggatttg attgtcatcg gctgtcgatt tcagccagcg taacgccccg
     7381 ccccagtcga tcagttgctc gccgggtaaa tccatcatcg gcgcatcgct gggtaatgaa
     7441 atgcgccata aggtacctgg taacgagaag aacggcagtt gttgttcacg caattgctgc
     7501 cagaactgac ccgcaacctc ttcgccaccc agcagttcac gcgctgcttt taccgatcct
     7561 tcgccgccct caaggcggat ccacaacgca ttgtcgaagt aacataagcc actaatgggt
     7621 aatggctgga gttgccactc ggcgatttca ctcatggctt cttgcaggct gatttcccga
     7681 cgcaggctca gagaggcacg cggtcgcggt aacactttca ttgagatttc agtgagcacg
     7741 ccaagacaac cgtagcttcc ggccattaac cgtgagagat cgtatccggc aacgtttttc
     7801 atcacttcgc caccaaaacg cagatgtttt ccagtgccgg taatgatgcg cgtgccgagg
     7861 acaaaatcgc ggaccgaacc gctccacggg cgacgcggcc ccgccagccc gcaggcgacc
     7921 atcccgcccc aggtggcttc ttcaccataa tgcggcggct cacagggaag catttgcccg
     7981 gcgctctcca gcgccgcttc aatcgccacc agcggcgttc cggcacgagc ggttatcacc
     8041 agctcggtcg ggtcgtaatt aacaatgccg cgatgacaac gcacatccag cgtttgcccg
     8101 gtgactgggc gacctaaaaa ggctttgcta ttgctgccct gaatcaccag cggcgtttta
     8161 tcgctaattg cctgattcac ctgctccagc agcgcctgac tgtaatcaca ctcgcgtagc
     8221 atcaaaagcg ctccagttca ggaaaaggta aatgaccgtg atgcacatgc atggcaccaa
     8281 attcagcaca gcggtgtagc gtgggaatgt ttttcccagg gttcagcaaa ccatcggggt
     8341 caaacgccgc cttgaccgca tggaaggtcg tgatttcatc gctgttgaac tgggcgcaca
     8401 tttgattgat tttttctcgc ccgatgccat gttccccact gatgctgccg ccaacttcaa
     8461 cgcagagttc gaggatcttc ccgcccagct cttccgcgcg ggcaaattca ccaggttcgt
     8521 tggcatcgaa aaggattaac gggtgcatgt tgccatctcc ggcatgaaag acgttggcaa
     8581 cacgtaaatc atattgctgc gataaacggg caatgccttc cagtacgcca ggcagggcgc
     8641 gacgcgggat ggtgccatcc atgcagtagt aatccgggga gatacgtcct accgccggga
     8701 acgcattttt gcgaccggcc cagaaacgta cgcgctctgc ttcgtcctgt gccaaacgga
     8761 cgtcagtcgc gcccgctttc aacaagatgt agttaacccg ctcgcagtct tcctgtacgt
     8821 cagactccac gccgtccagt tcgcataaca aaatcgcttc ggcgtcgacg ggataaccgg
     8881 catgaataaa atcttccgcc gcgcggatcg acaggttatc catcatctcc agcccgccgg
     8941 ggataatgcc attggcgatg atgtcaccaa ccgcaagtcc ggctttttct accgagtcaa
     9001 agctggctaa cagaacccgc gccacgggcg gcttcggcag cagttttacc gtcacttcgg
     9061 tggtcacgcc gagcatacct tccgatccgg tgaacagcgc cagcaggtca aaaccaggtg
     9121 aatccagcgc gtccgatccc agcgtcagcg cctcgccgtc cagcgtttgc acttcaattt
     9181 tcagcaggtt atgtacggtc agaccatatt tcaggcagtg gacgccaccg gcattttccg
     9241 ccacattgcc gccaattgaa caggcgattt gtgaggaagg atccggcgcg taatagagat
     9301 tatgcggcgc aacggcctgg gagatcgcca ggttacatac gcccggctgc acgcgcgcgc
     9361 ggcgaccaac ggggttaatg tcgaggctct ctttaaagcg cgccatcacc aacaacacac
     9421 ctttttccag cggcagcgcg ccaccagaaa gcccggtgcc tgcaccacgg gtcaccaccg
     9481 gtacacgcag gcgatggcag acagccagaa tcgctgtcac ctgttccatt tgcttaggca
     9541 gaacaaccag taatggacgc gtgcgatacg cgctcaaccc gtcacactcg taaggaatga
     9601 tctcctcatc ggtatgcagg atctcaagtc cagggacatg ctcacgcagt gccatcagta
     9661 ccgatgtgcg gtcgacatcg ggtaaagcgc catcaagacg ctcttcgtac aagatgctca
     9721 tgagtaggct tcgctttgtt gtgtggcagc tgatttttgc gcgctgcttc tgtgaacagt
     9781 tattaagcgg gcttttcgtt ttcgtctatc tctttagcta ccggtcagac catttttttt
     9841 ccagctctgt gaccttgtct tggttaactt aatgttaaat tgatgtaaca taatcacttg
     9901 cgtgatgtgc gtgttttgcg agttaagaac agaaaaattg gtcctacctg tgcacgaggt
     9961 ccgggaatga aagatgaacg tcgccctatt tgcgaagtgg ttgcagagag tatcgaacgg
    10021 ttaattatcg acggcgtact gaaggtcggt cagccgcttc cctcggaacg tcgactgtgt
    10081 gaaaagctcg gcttctcacg ctccgcactg cgtgaagggc tgaccgtgct gcgcgggcgc
    10141 gggattattg aaacggcgca gggtcgcgat tctcatgtcg cacggcttaa tcgggtgcag
    10201 gacaccagcc cgctgatcca tctgttcagt acgcagccgc gaacgctgta cgatctgctc
    10261 gacgttcgcg cattactgga gggcgaatcg gcaaggctgg cggcaacgct gggaacgcag
    10321 gctgattttg ttgtgataac ccgctgttat gaaaaaatgc tcgccgccag tgagaaccac
    10381 aaagagattt cgctgatcga acatgcgcag ttggatcacg ctttccatct cgccatttgt
    10441 caggcttctc acaatcaggt gctggtgttt acgctgcaat cattgaccga tctgatgttt
    10501 aattcagtgt ttgccagcgt aaataatctc taccatcgac cacagcaaaa aaagcagatc
    10561 gatcgccagc atgcgcggat ctacaacgcg gtgttgcagc ggctgccgca cgtcgcgcag
    10621 cgcgcagcgc gcagcgcgcg atcatgtacg gaccgtgaaa aagaatctcc acgatatcga
    10681 gctggaaggc caccatttga ttcgctcggc ggtgccgctg gagatgaaca aggtgggaat
    10741 gtgagcagag ttttacccta tccccaaggc gaggggaccg cccctgcact taacaggatc
    10801 tggtgttaaa tctgttcatc tataccctct cccgaatgac cgcccctgcg ctaaacagga
    10861 tctggtatta aatttgcttt tcatacaatc ggtaacgctt gtacggctcc gccccaatgc
    10921 gttccagcat gttattcatg cctgtattgg tttcgaggat ccattacatc tccagcgcat
    10981 cgatcttccg gcgggcaaac ggatcgcgta aggcttcaat caataacagc gcaatcaccg
    11041 ggccgatgcg gctgaactga tactcgtcgc gcacgcccat cagcggtact cgcgcagttc
    11101 gcacaccgct gactttcaaa cgccacagca attttgccca gccgaaggga aagagcgatc
    11161 cgttcagatc ggcaatcgcc tcgttgatgt taggcaagcc gacaataaac gcgcagggtg
    11221 cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta agttggtcgc
    11281 ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc tgccagccag
    11341 agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga ttgatgcagc
    11401 gaatggtcac ctttttgcgc acctgatcca tccgtttttt tagcgccgga gagaaagtga
    11461 gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa cccagttgtt
    11521 caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt ggtgtgtcaa
    11581 aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc ccgcagatct
    11641 tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg gcaaaatcct
    11701 gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg taacgctcac
    11761 ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct ttttttgcca
    11821 cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa tgctcgttgc
    11881 gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat gacggaaaag
    11941 cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc atcagcagat
    12001 cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt tgcaacagca
    12061 acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat agtattaatt
    12121 aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt gtcgatttaa
    12181 ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt cataataaaa
    12241 ataaaaaact caaactcatt atttaaatca atgttcattt tcacaccgat gaaaatacta
    12301 tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta tttttgatat
    12361 ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta cctatgcgct
    12421 atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt agagccggaa
    12481 tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag acattaaaaa
    12541 cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa ggcgaacgcg
    12601 tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc tgtcagtatg
    12661 ctggcttagt cgccttcccg ttggcgattc caatgggcgt cggtcagcgg gattcctgga
    12721 gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc actggtgatg
    12781 agtagttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat gttttaagcc
    12841 acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca gttccgaacg
    12901 atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc gtcattatca
    12961 cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt aaattacgcc
    13021 ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg gtcggctttc
    13081 tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag gattttgcca
    13141 tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt tccgttgcgc
    13201 cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc gctgaactgg
    13261 atctttccta ctggcgcgtc gctggtattg gtgctgagcc gatctccgca gaacaactcc
    13321 atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc atgccgtgct
    13381 acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc ggggttgtgg
    13441 ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg ccgggtgcag
    13501 agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa catggtattg
    13561 aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat atttgcatct
    13621 ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac gagattgccg
    13681 cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat ctgtatgtca
    13741 ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg caggatattg
    13801 aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct tttgttaccg
    13861 cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa cgtcgcgggc
    13921 agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc gcggctatcg
    13981 atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc cgtgcggaag
    14041 cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa tccctggcat
    14101 gaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata ttatcgataa
    14161 cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg ctcacgtcaa
    14221 tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta gcgagctggt
    14281 tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca aagaagagat
    14341 tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa aagagagcgg
    14401 cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg agctctcctg
    14461 gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg acgaaattac
    14521 gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt taaaaccgct
    14581 gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata cacaactctc
    14641 ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg ccgaaactgt
    14701 acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact ggcaacgcgt
    14761 acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca ttcctctgcc
    14821 ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag gtaaagaacc
    14881 tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg caagtaataa
    14941 ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg cattacgcaa
    15001 cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt aaatcgtgaa
    15061 atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg tgtggaaatt
    15121 aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt gatggatctc
    15181 ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct gctgaatgtc
    15241 aaaccccctg cgcaattaat ggaaactcta cttccatggc tggagaataa ataatggggc
    15301 tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac aacgggttaa
    15361 caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg atcggcaata
    15421 aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat catgatgcca
    15481 tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt tctcgtatgg
    15541 caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag tttttcaatc
    15601 gccctaccgc tatcgttttt tcaaccggtt ataccgctaa tttaggcgtc atcagcgcac
    15661 ttgccgacca taacgccgtg gtgttactgg atgccgacag ccacgccagt atttatgacg
    15721 cctgttctct cggtggcgcg gaaattattc gtttccgcca taacgatgct aaagatctgg
    15781 aacggcgcat ggttcgcctc ggtgaacggg cgaaagaggc gattatcatc gttgagggca
    15841 tctacagcat gctcggtgac gttgcgccgc tggcggaaat tgttgatatc aaacgtcgtc
    15901 tcggtggtta tttgattgtc gatgaagcgc attcgtttgt cgtgttaggc gcaacggggc
    15961 gcggactggc tgaagctgtc ggcgttgaag atgatgtgga tattatcgtt ggtactttca
    16021 gtaaaagtct ggcctcgatt ggcggttttg ctgtcggttc ggaagcgatg gaagtgctgc
    16081 gctacggcag ccgcccgtat atttttaccg cctctccttc tccgtcctgc attgcgaccg
    16141 tgcgctcgtc gttaagaact attgccacac aaccagagct gcggcaaaag ctgatggata
    16201 acgccaacca tctgtatgac ggtctgcaaa aactgggtta tgagttaagt tcacatatta
    16261 gcccggtagt gccagtaata atcggttcga aagaggaagg tttacgcatc tggcgcgagc
    16321 ttatctcgcc gggcgtgtat gtgaatctta tcctgccgcc tgccgcgcct gcaggcatca
    16381 cgctgctgcg ctgtagcgta aatgctgccc acagtcgtga gcaaatcgat gccatcattc
    16441 aggcatttgc tactctgaag cagtaatgtg agcccgccgt ggtgtttgcg ccacggcggg
    16501 tttaatgttg tctgtcatac caatacacca ggctcaacgc caccaacaca aacgctaccg
    16561 gtaaaccgag cgcggtaaca ggtgcgctta acgcaaatag caatcccaga ttaaccatga
    16621 tttgatagct gatccaggtg agtaagccca cgatgacgcc tacagcgaga cggctaccca
    16681 tcccaggcga acgcggggca ctaaaggtaa acggcacagc cagcaaaatc atcgccaggg
    16741 tcaggatggg ttgccccagt ttttcccaca gagcaaggcg gtattcgctg ctgggttgcc
    16801 cggtttcctg caaataatgg atgtaatgat taagctgacg gacagaaaaa gtattccccg
    16861 gcattgataa ctcttcaaga ttcatactgg tgaaggccga ttgccaggca agattatcta
    16921 atgtttccag cgtttctttg ccattcagcc attttttatg attcacacca tgcaggaccc
    16981 acgttttgtc gtcttcaatg gtggcactgc gtgcataaat gtaggattca agcgagagat
    17041 cgtcgcgata atgaaatatc tccacgccca ccggctggcc ctgctcgttc agcgatttca
    17101 ctgtcacaaa ttcattacca cgcctggccc acagcatatt gccggtaatg tcatcgtcct
    17161 cccccaacgc ggtggcggtc gatttgattt gcaacgcctg ttgctgcaat ggcgacgcca
    17221 cccactcatc tatcgcgcct aacgaaacag tccacaatat cccggcaacc agcgccacca
    17281 gtgcaatacg gaagatagaa aaccccgtgc tgcgaatggc ggtaagctca ctgtttttcg
    17341 ataactgccc cagtccaaca ataccaccca ataacgcgat aaacggcgaa agttcgacca
    17401 gtgtgcgcgg taaggtcatt agcaccacca gcaccgcctg agtccagcga taaccgcccg
    17461 ggctgacacc atccagttcg ttaatcaggt tgaaggtggt aaaaagcggc agcaatagcc
    17521 ctgcagcggc ggcaaaaccg agaaagagat gacggattaa atagcgactg aaaacattca
    17581 tcgggaaaat ttctgcagca aagagaagtc acgtgcgacc agtatcagca ggcccattag
    17641 catgagtccg ggcactaacc acaaaccagg aatgagggga atcgcgccgt tagccaccag
    17701 tgtacggcag acgtcgccgc cataaaaaat ggcaataaac agcaacgtca gtggcaataa
    17761 cgttgaaaat cgcccttgcc gcggttttac ccgacttaat gaaatggcca gcagcgccat
    17821 caacaatgcg gtcaggccac ggctttgtcg ccattgcagt tcggcatgat cggcaggaaa
    17881 ggcggagcgc gccagctccg tgaccgattt cgctttgcgt ttgacgttcg ggctttgatc
    17941 cagcggattc agatgtaatt gcagattacg gtagatctgt tcgttgtcgt cacgcccctg
    18001 atgatcgaga agataggcag tgccgttgtg caacataacg gtcggtttct cgggtgatgg
    18061 gtcaaccaca tcaaccgaac gggcgcggaa aatacgggtt cgattggcag tagaagtata
    18121 aatcagcgcg tcagtcaggc gattattatc ctgatcaacc gtctgcgaaa ggatcattcg
    18181 tccgttatcg ttagtgttaa atttctttgc ccgcaactgg cgaacatcca gctccgactg
    18241 cgactgctgc tccagttgat aaatctgcgc ataagcccac ggacgaccat acatcgacag
    18301 aagggtgaca aaaatactta gcggtatcgc cagataaaga acggctttgt acaagcgtcc
    18361 cggactgccc cccgccgcag agatagcggt aatttccgag tcggtataca tctgccctag
    18421 cgtcacgcca actgacacat acagaccaac aggtaacaac atctccagtg caatcagtac
    18481 tttgtagaaa acgatatcca gcacaacatc aagagctaat gtcccatttg ccgcttcggt
    18541 cagataacgc tgtgcggagt agctggcgaa aataaagatc agaaaaccga cgataatcag
    18601 caccagacgg cgcgtaccac gcatgatgta gtgttcaacc agcttcatct cactaacgcc
    18661 ctcaatcctt tgagtttttt attcattgat cagtttctgc gccaacgcaa tgacatgatc
    18721 tacctcttcc ggcgtcaggc tgccgtcctt tgcccactga atttgcccgg ctttattcag
    18781 caccagaatg gttgaactct gctccggcaa gcgccaggca acgcgtccct gtccattgcc
    18841 gtcaataacg aattgcgccc acggatagcg gcgtttattt ttttcgattt tgccaacaac
    18901 aaaatagcca gtaccaaaga tggcatcatc ggtgttcaca atggtggtgg gctgaaaacg
    18961 atcctgcgga aagtttgccg cttcaaccgc tttaattaac agtgagttct ttttcttcgc
    19021 cgacttacgc ccggcgatat actgaataat gcggactttt cccgctaatt gtgtgctgtt
    19081 ccacttttgg tagtgaattt cgtcgtcgct attcagaagc agctcgccgc cttcctggat
    19141 ataaacaggg gtaaccgttt tgccagtgac gaaattgtgt gcctgcacac ccggggctaa
    19201 gagcatgata atcaacgcaa taatcaaacg agatgacatc attgctccgg ctatagcgga
    19261 aaacgcgaaa tcagcagctc taagcgctga caaatatcgc tatccagcgc caacggtgca
    19321 taatacgact ggcggttttc catatcatcc gccaccatgt cgtcgtgttt atctcccatc
    19381 aggccattaa tggcggcgag gaaacgggaa atctgtaacg cctctttatt ttcaggatca
    19441 agcgatctta aggtgtcgta ataagaaacc tgctggctga tgatttttcg cacatcatga
    19501 aattccctgc cggttagtcg cggtttggca ataatctctt tcagggtatg catttgcttt
    19561 tgccgatagg caataaaact gcttacgctt tctaactggc tatggcgtaa tgaatagttc
    19621 accaacgacc agaccggaga gcttaaataa atccgtaaca ggttgccgta atacgacaca
    19681 atatttttct taccgttacg aaagccgtcc tgaaaacgcc ccagaaaaac ggtcgttttg
    19741 ccgaataaaa atccggcctg atgcttcttt aaatacagcc gttgcgcaaa acgcaggtgc
    19801 ttgtagcgcg ctcgcatgta tttaaattgt ttgcgctcat cagcagtcag ctcatcgcct
    19861 ttggcttgtt tgcgaactaa acgcagtaat tcctgacgat caacgccatt gagccagaat
    19921 tgcaggcata acgcgtaatt atcatgaatg atttcggtgg tacaacttag tgcaatagtc
    19981 ggcggcaaac taacctgtgc atcaacgaca tcatcttctt caatcgcaac aaatagacgc
    20041 tcgctcgcct caagcgggaa atattttctc atcatcagga tttctctccg gctgcgttat
    20101 aaagcaagga atggcgctga aaaaatgcct ttaaggttgg aattaacgag agcgccacgt
    20161 ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac agatgacaga
    20221 acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg agaaaagcgc
    20281 gctgttttcc cgccatcaat agcaatgact cctgcgggaa gcccagcatc gaaatcacaa
    20341 ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg ccaaacacca
    20401 gcgaaatgag cggcttgccg acgatcaaca ccgcgagcgc caccagaata ccgattccgc
    20461 ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc ggatctaaac
    20521 gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg gcggcgtcga
    20581 agaatgtcat ggcgatttta aataacccag cggcagcggg tcctaacacg atccccacca
    20641 acactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg gttgaccaga
    20701 caaaactcca cgcgccttta atgtgtcggg cagactcaaa cagattcaat ttgaaggcgt
    20761 tatggatatt tctacggcgt aattcgcgcg cggcaaacca ccagtacatg gtgccgccaa
    20821 ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca aaataccagg
    20881 ctgcgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca gcaatcaaat
    20941 caaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt gaaggaatga
    21001 gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat gaatgggaaa
    21061 ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg atatccagcg
    21121 agaaggaaaa tgagacgata ttgcgaaatt gctgcggatt attgttggtc aaggctggcg
    21181 ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg atcgacttgg
    21241 cgtacgattg cacaatcacc agtacaccaa acatggcggg cgtcatccct ttaccggcac
    21301 acgagagcgc caacagcccc agcaaggcgc tctcgacatt actggagcct aaccaggcgc
    21361 tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg ttaaaacccg
    21421 ccaattcagc ctgaccttat cgttgatagt aaaaaatatc ccgccagcct taagttaaac
    21481 ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtacctgt tccagcactt
    21541 cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag ccaatcgtcg
    21601 acatgacgcc gattttgtcc tgcagctcgg catactcatg gtcaggcttg cgggagatgg
    21661 cagtatcaat atcaatcccc aaacgaatga ttaattcagg acggtattgc gccatttttt
    21721 ggtataaact ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc gcacgttcga
    21781 cgccgatccc cggtccgtca taataaaagc ccgaaatttc agcctgcggg aagcgatcgc
    21841 tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc gccattcttc
    21901 gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg gttttcatgc
    21961 tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca accaacggca
    22021 atcgtttgat tttgtcgccg tcttcgccgg agagcagccc cagatagcgg cgctcggttt
    22081 gccagtgttg ttgcagcgat ttcaccaggt cggtggtcag cgtggattta ccggtgccat
    22141 cacacccgac caccgcaatc agccccggaa tgtagttggg ctgcggcgcg gttgtactat
    22201 taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca ttttggtgat
    22261 caggaaagcg cggcgtgaat tgcgcgtagt gacgcttgca taatttcatc ggctggttgc
    22321 cgaccatcca gttcaaggat cttggcgcca ttgaatgtca gttgcggcgt aacggcgatt
    22381 ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt ttgttcatca
    22441 atgccaagac gaatcaacaa tacgggtaaa taagacgcca tccattggta cagcttcaac
    22501 tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc caattgcggg
    22561 ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat gagcagatag
    22621 ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca ggaaagcaga
    22681 tagataacca gcgcagtaat attgccaggc ggtgttgagg gcttttcgtg tacatgtgcc
    22741 gctttacttc gcagataaca cccacaaggt gcgccaataa cagggagctg tgaaatccat
    22801 tcgccaattc gcccggacga ttgcccgaga taaatgtgtt ctgttggcat tcttgctgcc
    22861 agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca tcctacaatg
    22921 gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt cacgaaaaac
    22981 ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc cgttcaacct
    23041 tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa aaacatatta
    23101 accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc agtcaataac
    23161 acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg tgtgtgaaca
    23221 tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag gaaaacaggc
    23281 cggaaatgtc ggtcgtgcag tgacaaaatt acctttgatg ggaaaatcct tacataaaac
    23341 aattgaacga aatcaggtga aaacagcaaa aaaattgccc ggaccagttc cggcgctggt
    23401 aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg cctgtcgtcg
    23461 tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg gcgcttacga
    23521 tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc tggcaagcca
    23581 ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca tcaaactcaa
    23641 tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat cgctggcgag
    23701 gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg atatcgatgc
    23761 caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg attttatgac
    23821 cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc gggcattttc
    23881 aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga aaaattgccg
    23941 ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc tgtaacccac
    24001 cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt gtggagacag
    24061 gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt gccgccatgc
    24121 cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc gctacacgac
    24181 gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg atccacaccg
    24241 gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg ttctggtctt
    24301 ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg cacagcataa
    24361 tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc atattgcctg
    24421 gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga cagtgaaact
    24481 ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc gcaatcaact
    24541 tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtaacg gcatcgcggg
    24601 tacgggtaat ttcatagccg gacgcattca tattgacgac gaagccagca ggggcaatcc
    24661 ccaccagtac cagcattacc aggccgatcc ctttctgccc gtcgttcacg ccgtgcgaaa
    24721 acgccacgcc tgcagcggaa acaatcagcg caatacgcgt ccagaatggc ggtttacgtt
    24781 tgcccttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc tttttcgtcc
    24841 cactccagaa acggcgcagc aggaaaatca gaccacccgc aatgaccagg ccgacgatag
    24901 gggaaacaat cagcgaggag aaaattttgg ttacttcacg caggtttaac gcatccatca
    24961 ccgatgagcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca ccaatcaagg
    25021 tgtgcgaact ggaggccggt aaaccaaaga accacgttcc caggttccag ataatcgccg
    25081 ccagcagcat ggaaaagacc atcgccagac cgtgagttga ccccatattc agcaacaaat
    25141 cggttggtaa catatggaca atggcatagg caacgctaag tccgcccaat aacacgccaa
    25201 aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ctgcatggca cgagtataaa
    25261 taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg tagaacaaca
    25321 caaatgccag agcaagtaat aacaaaagcc ccgtatatat atcaaggcca acaaataaat
    25381 ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat cagtgacttt
    25441 aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt atttcttgtg
    25501 tattagacaa taagcattca ttcaaaacct ttagaaataa tcaaaacaca ggtattccac
    25561 tggttaatat attcacatat gaaatgaata aatattggaa tatataaata ttgaatattt
    25621 tgattaatcc ccgtactaat tattcttcat aatcagtacg gggttgctac aacatgtatt
    25681 actttttcac cacaattaac ggttcaatat cactctcttt tttgatgacc agtgattcat
    25741 caccgcgcaa acacgtccca ccgtagttgc cgccaacggt gaaggtacat acctgaatgt
    25801 atttaccgtc cactttcggc aaacaccaca gttgctgata gatgtttttc tgctcggcaa
    25861 atttaccgct ggttttgtcc agcacctctt catgatggcc gaccaggtcg atattgctac
    25921 cacagcgacc ggcgatcggt ttcactgcgt aacctgtttt caccaattca tcattaacag
    25981 tgaaatcggt atccagcagg taacgatggt gcgggaacag cgaccagagg atcggcagaa
    26041 tcgctttgtt gccggggatc accgtccaca gcggctcaaa gaccagcact tccgggcgca
    26101 gcaatacgtc gataagacgc acttcgtttt gcggatgacc ggtacggatt ggcaccgcag
    26161 caaactcacg gtcgctaact tcacgaatct gatcaaacgc ggtttcccac gcccaggttt
    26221 tccacacgca gttaaccagt cgcccttccc catcaatcag ttgcccggca gcatcccagc
    26281 ccagttcatc cagcccgcgc aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct
    26341 ccataaactg cgcgtgatag ttttcctcga tatctttgtc ctgcatgata tggacaaacg
    26401 gacgtgcacg actgtgtttc caggcaccag ccagttcgtt aatcagccct tccgccggat
    26461 tgaagccgtt gcctttatag ccctgctccg cccaacgttc gaggatcaag cccgcttcgg
    26521 tatgacagga ggcggagtcg gcgttgtatt cgtaaacctt caggccacgc tcatccatgc
    26581 agaaatccat acgaccagtg atcatatggt gacggcgacg ctgccaggag agacgcaaac
    26641 gtggccagag gattttcgga atgtcgaaca gcgccagcag gttgtcatct ttcagcacct
    26701 tgtcggttgc gtgaagatac atcagatgca gctcgttggt ggctttaatt agctcctgct
    26761 cggcactctc ggtaatggtg tagtaatgat acggatcctg attgatcacc tgaccgttgg
    26821 cctgcacata ggcgttttgt agcggatctt tttcatccag ccatttaccg tcaaactggc
    26881 ctttgttttc cagacgcgct ccactgattt tcagcagctc gcctgcaatt tccggctgcg
    26941 gtaagctgta ttcagtatct tccgtctgga tcatccagcc cagaatggtg gtgtcatcaa
    27001 aagtgtcttt aagggtatag cagccgtttt cgaccaccat ctccagctcg cgcgtccact
    27061 gttgcccttg cggcaacggg gaatgaatca cgttctgttc cgcaatacgg actttgttgc
    27121 catgcaattg ggtaatgatg gcgacatggc cagtgtcttt aaattcaccg cctttatccc
    27181 agataagaag cgcacccgcg accggcgcac gcggcgagcc gttaggaaat gcctgcaatg
    27241 gcaggatgtt gtcattaacc acttctcgca ggaagcgcag cgagaaaatc tcccacgcca
    27301 tacccacgtc agtaaagacc acaccgtaat tcagaaagag aaaacggcga gcaaattcaa
    27361 cgcattgcca cttgtggccc atatattcgt cgtcgatata gctacggaat acggcgtcat
    27421 cttcgtattc ctgcggatcg agagaactgt aatctgaaga gtagattgct accccacctg
    27481 gggcgtagcc caataatgtc ccgaacgggg catcctggct ggtcgttcct ttgctcatca
    27541 ctttaccttt aacaatacaa cctaagcagt tggcgtaatt gttgtgctct gattacctgc
    27601 tcagcgacat taaccggaca gaggtcagac taatcataca cctcatcgca gcggctggcg
    27661 caaagggtta aaaaattcac attctgtaca gcaagtgacc tgctacactg cttcaacact
    27721 accactcaga aggcaactca ctatgacaga caatacttat cagcccgcga aagtctggac
    27781 gtgggataaa tccgctggcg gcgcgttcgc caatatcaat cgcccgtttt ctggtccgac
    27841 gcatgaaaaa acgctgcccg ttggcaaaca tccattgcaa ctttattcgc tgggaacgcc
    27901 gaacggtcag aaagtaacga ttatgcttga ggagctactg gcgctgggcg ttactggtgc
    27961 agagtacgac gcctggctga ttcgtattgg cgatggcgat caattctcca gcggctttgt
    28021 cgaagtgaac ccaaactcga agatcccggc gctgcgcgat catacgcata atccgccgat
    28081 ccgcgtgttt gaatctggtt cgatcctgct ttatctggcg gagaaatttg gctacttcct
    28141 gccgcaggat ctggcaaaac ggactgaaac gttgaactgg ctgttctggt tacagggtgc
    28201 cgcgccgttc ctcggcggtg gttttggtca cttttaccat tacgcgccgg tgaaaattga
    28261 gtacgccatc aaccgcttta ccatggaagc caagcgcctg ctcgacgtgc tggataagca
    28321 actggcgcag cataagtttg ttgcgggcaa tgagtacacc attgcggata tggcgatttg
    28381 gccgtggttt ggcaacgtgg tgctcggcgg cgtgtatgat gccgcagagt ttctggatgc
    28441 gggaagttat aaacatgtgc aacgctgggc gaaagaagtg ggcgaacgtc cggcagtgaa
    28501 acgtgggcgt attgttaacc gcaccaacgg gccgctgaac gagcagttgc atgagcgcca
    28561 tgacgccagt gattttgaga caaatacaga agataagcgt caggggtaag ggttggtgtt
    28621 cgtcgcagca agccatccag gccggataag gcgttcacgc cgcatccggc aatcgtgcac
    28681 aatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaagctaatc atcacgcgct
    28741 ggtaatgtcg taatccattt ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc
    28801 gatgatgctc atggcaaatc cgacgtgtac cagcacccac tggcccagca gatcggcagg
    28861 gttaccttcg caaatcaggg cgatattcac gtcgcgcttg ataccacata cttcaacctg
    28921 cgcaagctgg tgaatatctt caccgacagc cagcacctgg cctggaacgc caatacacat
    28981 tgttaactcc ggttatttcg attgccggac aaacgcgaaa aagaaccttt agcgggtgtt
    29041 ccctctcacc ctaaccctct ccccggaggg gcgaggggac cgtccgtgcg ctttttaggc
    29101 gttgtcctca gtcttattca acttcaatac ttttgacgat cagcgaatcg ccggtatcga
    29161 cacgcaaccg ctcgccgtga cagagcggac actgcgcatc gtgctgatga atctccacca
    29221 cctggctgca atcccagcac caggcctggg cgggtttata gacgatatgt aaatcgcacc
    29281 cttgcgccac cgttccgtgg cagacaattt caaaactaaa acggacggcg ctctcctcaa
    29341 cgcaggagag cgcgccaatt tccagccaca cggcggtgac gcgcttaaca tcgtgctgct
    29401 ccgcctgccg ttggataatt tcaacggcgc tctggcaaag cgacaactca tgcattttcg
    29461 cccctcctgc ggccaaacag cagggcgcga cgccctgcgt gtggtacatc cggattggtg
    29521 actggcaacg aaaggatcat tcgtgcgcag tcatccgtca ggcgttggcc ttcttcaatc
    29581 gacatgctgt gcgaaagcgg cgacatcagc gaacaggaga ggtattgcga aacaccgtcc
    29641 agttcgccaa cggtaaaggt catggtgcca tacggcagtt gcagaccaat tttttcactc
    29701 actttgcgca gcggccagag ttgatccggg ccggggaaga taactgcact cagcatccac
    29761 ggggtgatca cacaccccgt ccactgacct tcgaacagcg taaaatcaga aacatacacc
    29821 ggcattgaag gatgcagaaa agagagatcg tgcatcgaac gccgggcaat ttcttcaaac
    29881 gctgcctgta cttgcgcctt cggggaggtc tggaaacctg ctatctcttc agtcatgaat
    29941 cgcctcccgt gggatggctt ccacgcctga ttcgcgcagc gcagccagaa ccagctcaag
    30001 cgcaggttca atcattgctt caaccgtcgg cgttaagccg atgtgtggct ccagcgattc
    30061 cgggatcacg ccgaccaggg tcagcttttt cggaaactca ccggtgaagc gcagggccga
    30121 caatacgtcg gccaggccaa gctgatgcgg agagattttg ttggtaaaca acgccggaac
    30181 ttcttcatcc cgcaggatca tcatcgttcc cggcgtgttc tttttcgaca caatggcatc
    30241 cgcaataatc agatgatcgc gatttgccat gtcgccaagc agctccattc ccgccgtgcc
    30301 gccatcgagg atctcaacat aatccggcag aatgtatcgt tgctctaacg cttcgacaat
    30361 ccgcacgccg atggcttcat cggtcagcaa aatattgccg acccctaaga ctaaaatacg
    30421 cattacagaa ccttcactga aaccacttcg ttgccgtcgg catccactac gtgtaccgca
    30481 caggccatgc acgggtcgaa ggagtgaatg gtacgcacca cttccagcgg tttattcgga
    30541 tcggcaaccg gtgtacccac cagcgactgc tcgtaaggac cgacgtcatc attgaagtta
    30601 cgcggaccag agttccaggt tgatggaaca accgcctggt agttgctgat gataccgtct
    30661 ttaataacca tccagtgaga gagcataccg cgcggcgctt cgaggaagcc aacacctttg
    30721 aactcacccg ttgccggaat gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata
    30781 ttggtgatca gtgcactgta ttggttttgc aggatatcct gcaattcaca gcagtgaacg
    30841 gtacgaccaa taatacggcc cagcgtggag tgcagctgtg ccacttccag cgtgttgcca
    30901 gtcagtttct gataaatcgc aacgatttca ttcagtttgt tctgggtaga ttcgcgacct
    30961 gccgccagtt tcaccagcat attagccagc ggacccactt ctaccgtttt gccgtagaaa
    31021 gtcggtgatt tcacccagga atatttcccg tcgtcagacc aaccatcata agccggaatg
    31081 gtggtacctt cccatggcgc ctgtggcgct tcgtctttat accaggagtg cttcgcgctt
    31141 tcctgaatgc ctttgatcag gtattcatcg gaatgagaag tgatcggacg atacgaggac
    31201 agatccgcat tctcaatgta gccgcccggg aacaggaagc tgccgttttt gctgtcggtc
    31261 gggaattccg gcacgctcag gtagttcacc gcacctttac cgcgcgtcag ccattccggg
    31321 tagaacgcgg cgataaccgc ggtatcaacc ttataaacct gctcaacaaa gtcgctcagt
    31381 ttgtcgatga aagacttgat gtacatcagg cgctcaaggt tcaacacgcc caacccgtcg
    31441 aggttgattg ggttcgcgac accactcact gccaggttct gaatgtgcgg cgttttaccg
    31501 cccagcagcg ccacgacgcg gttagcgtca cgctggcact ccagcgcctg caggtagtgc
    31561 gctaccgcaa tcaggttcac ttccggcggc agtttcatcg ccgggtgacc ccagtagcca
    31621 ttggcgaaaa tacccaactg accgctggca accagatctt tgatcttgtt ctgaacttta
    31681 gtgaactctt ccggactgtt cagatgccag gtcgaaacgc ctttcagcat ttcggaggct
    31741 ttggttgggt cagcttgcag tgcagaagtg atgtccaccc agtccagcgc cgaaagctga
    31801 tagaaatgaa cgatatggtc atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat
    31861 tgcgcgttaa ccggaacgtc gatattcagc gcactttctg ccgcacgaac ggaagacagc
    31921 gcgtgagtgg tagtacatac gccacagata cgttgcacaa tcatccatgc atcgcgcgga
    31981 tcgcggtttt tcacgatctc ttccatgccg cgccacatgg taccggaagc ccatgctttc
    32041 gaaacgacgc cattttcgat ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc
    32101 ggatcaatag taattctctg gctcatgctt tgctcgcctc atgacgatta tgatcgtttt
    32161 gttttaaagg aggaagtatc ggcagtagac gaatgagtac gatgtaagcg caaatctcaa
    32221 tagccacaaa accaatagaa atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc
    32281 cgcccgggtt gaatgccacc aacgaatagg tcagacgcca ggttgcacaa cctaacagtg
    32341 cgctcagtgc tgacaggaac agcatgcggg aatcattacg cagcttcgcc acacgcagaa
    32401 cgaccagcgg gaagagcatc agcaggactt caatccagaa catcacggag tagaagtcac
    32461 cggcaaacgc taacgacagc ttgtcgcgat agataagctc gccaaagcgc agcccgatga
    32521 aaatcgccag caacacactg atggtgttgg tcagcttgac gaacaggctc ttttcatccg
    32581 gaccgttgcc acgcagaccc gcctgcacca gtgaaccttc aaagatgaca atcgagaagc
    32641 ccatgatgaa cgccgtcagc agcgagaaca gcggcaacat ttcatagctc tgccagagcg
    32701 gatgcacctt gtagcccgcc gagatcatca gcgaccccat tgaagactgg tgcatggtcg
    32761 gcagcagcgc accgagcgcg atgatgaaga acatcacctt gttcagacgt tgtagcgaca
    32821 ccttccagcc cagacgttca aacagtgccg gagcaaactc cagagccatc acgccgatgt
    32881 agatggtcat acagaccgcc gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa
    32941 tgtagaagta cggcaggttc cagtagcgac ccacgtcgat agtgatcgac aagccaccca
    33001 gtgagtaacc aaacagactc gccaacagcg ccggacgcac cagcggatgg tattgcccac
    33061 ggttaaagac gtataccgcc cacgccagcg cccagccgcc acaggcaaag ccggtgccaa
    33121 tcagcaggtc aaacgcgatc cacacgcccc acgggaagcc gccgttcagg tcagagacag
    33181 agcccagacc gaacaccaga cgcttcacaa tcaggagcat acagatgacg attaacggtc
    33241 caaaaatcat gaccggttta ctgatgattt tgccgcccag cggttgtgga tcatgactca
    33301 tgatcgtctc ctccgtcgtg atggtcgttt ttggtgttgc gacgaaccag cacggttaag
    33361 cccgccagca cagccagtgg tagcatcatg cctttataca gggtgtgttg aatattttcg
    33421 gaacgcgcac cggtagaaag atcgtccagt ttcggcaggt cgagattttc ataaggcaca
    33481 cccgtcagta ccataacctg agtaccgccg ccctctttct cgccgtacag atgcggataa
    33541 tatttcggca ccgtatgcag gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg
    33601 tattcgctgc caggcttcag cgccagacgt tttttcgcct ccgccatcag ctcttcacgc
    33661 gtaccgaaaa tcaccgcgcc cgccgggcac acttctacgc agccaggcag accgccttta
    33721 tcgagacgtt ccacaccttt ctggttgcac agctcgcact tatgcagcgc accaaacggg
    33781 ttgttgtagt cgtacttcgg cacgttgtac ggacaggcga ccatgcagta acggcaaccg
    33841 gtgcacacgt ctttgtcgta atggacaatg ccggttttcg gatctttttt cagtgcagag
    33901 accgggcaca cagagacaca gttcggatcg acgcagtgca tacactgttt cttaatgtac
    33961 gcgtagccgt tctcctcctg gtctttgttg acccctgtgc cgctggtcca cacctgaatg
    34021 atgttattgg tatacggcga cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg
    34081 ttacgttcag ggaagttgat atcctgacac ttggtgacgc aagcctggca gcctacgcac
    34141 aaggtcgagt cgtacaacat ccccagcgat cccggaattg gcgggcggtt ttcagcagcc
    34201 gcatgactga cagacggcag cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata
    34261 aaattacgtc tgttcacggt tattctcccc gtgagtcagc gttatctttc ttttgctgac
    34321 gacccagttc acgcaccgcc atcacgctga caccggcaac cagcccaacc acaccgccga
    34381 gcaaaccaat agcgcctgca gagacgttgc cgccctcttt agcgttaaca tccggtttct
    34441 gtgaacgcgg agtctgattt tcgacgttgg caagctgatg gatgccttta tggaagccga
    34501 tacctttttc gttacagcca tagcacgggt gaccaatcgc caccggccac acaccgccaa
    34561 catcgcagaa ttgcagcgtt gagcagttgc cgtaagtttc tggcccttta cagccgaggt
    34621 ggtacaggca ccagccttcg cggtggcctt catcaccgaa ctctttggca aaacgaccag
    34681 catcgaagtg cgggcgacgt tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg
    34741 gacggttttt gtcatccagt ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg
    34801 cgaggaagtt gtacgggttc ggcgggcagc ccggaatatt gataacggtt ttgcctggca
    34861 gaacttcttg caagctgact gcgccagttg ggttaactcc agctgcggca acaccgcccc
    34921 acgcggagca ggaaccaata gcgataatgg ctgctgcgcc ttccgccgct ttgcggatgt
    34981 gatccacaat cggctcaccg gcaaccatgc aataaatacc gttatctttt aatgggatgg
    35041 aaccatccac cactaacaca tactgccctt tgtacttctc gagagcgtta tgtttgttct
    35101 cttcgacctg atgaccgaag gcggcggaaa gcacttcgtg atattccaga gagatagtct
    35161 ccagcacgag gttttctacc gttggatgcg ttgcacgaag cagagattcc gtacaaccgg
    35221 tgcactcctg cgcgccaatc cagataactg gcggacgctg cgggttagta accgattcgg
    35281 ccatctctgc agcggctttg ctacttaacc ccatggtggc ggctaatgct gcacaaagct
    35341 tcatgaaatc acgacggtta atgccgtgag aatggatgag ggtgttatct ccagtcattt
    35401 atagttattc cgttgcgagg acctggcata tattttgctt caatcgcaaa atcaataatg
    35461 cgatcgatgc gccatttacc acacatttat tatggttatc gatatcatga tactgcggcg
    35521 gggcgctaaa acgaagggaa gaagcattag tgtaattaat taaagcaaga taatacgtat
    35581 gtttgatcaa ttttcgaggc gataaccggc cataaaaaag cgcaacgtag aacaggaatt
    35641 attctcacga aagaaatcca ttaccatctc tttatccagt ccatagcggc gcgtcagaat
    35701 acccgcttcc caggcgctaa gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta
    35761 gcctaataca aaaccgcgtt tataatcggc acagaatttt gtcacattaa ccgcactgtc
    35821 ggcatgcgtt gcttttaatc ccgccattaa gcctttacca aaatggttat tcatcgttca
    35881 ccccattcgt tatatatcgc catatatagc gatattttag gcaaagaacg acttttgcgc
    35941 acgctatttt ttcatacttg tttaagacgt aatcccgtaa gagataaacc tatcaggaga
    36001 gatatttatc gaaccatttc agtgtccttt gccaggcaag atcggcggca gatttgtcat
    36061 aacggggcgt ggaatcatta tggaatccgt gattaacccc cggatagata taagcctcat
    36121 aaaccttatt attgtctttc aacgccgcct cgtaaggagg ccagccctcg ttgattcggg
    36181 tgtccagttc cgcgaagtgg agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg
    36241 gtgcctgacg accataaaac ggcaccgcgc aggccagttc cggatacgcg acagccgccg
    36301 cgttcgatac gccaccgcca tagcaaaatc cggtaatacc cactttacct gtcgcttgcg
    36361 gatagcgttg cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa
    36421 cctgctgttg cagctcacga cctttatcat catttcccgg ataacctcca acggaactta
    36481 atccgtcagg tgccagggcg atataccccg ccttcgccac tcgccgtgcc acatcttcga
    36541 tatacggatt cagtccacga ttctcatgca ccacccccac ggctggcgtt ttgccgctca
    36601 tttttgcggg tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg
    36661 tgatgtactc agcaacaatc tccgggtcgg taaactctac ctgagtcgcc agcgcataat
    36721 ttggcttgag caaatcaaac aacgctaatg ccgtcatccc gcccaccgca tacttcgccg
    36781 caaggttgag gaactcacgt ttcgaaattt tcccgtgaac gtaatagtcg tagtaatcca
    36841 acaactcttg tgggaaatct ttggcggtta aacgcggcat cgttgcattc ctcaactggt
    36901 gtttttttaa gcaaagcata agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc
    36961 agactacggg atacgcgctg gcgaatcgct aaactagaaa cattgtttcg aaattgaacg
    37021 gtggaaagga gaggtcatgg tctggttagc gaatcccgaa cgttacgggc agatgcagta
    37081 ccgctattgc ggaaaaagtg gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa
    37141 tttcggtcac gttaacgcgc tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt
    37201 aggcattacg cactttgatt tagccaacaa ttacgggccg cctccaggaa gcgcagaaga
    37261 gaactttggt cgcctgctgc gcgaggattt tgctgcttat cgcgatgaac tgattatctc
    37321 taccaaggct ggctacgata tgtggcccgg cccgtacggc tctggcggtt cacgtaaata
    37381 cctgctcgcc agcctcgacc aaagcctaaa gcgtatgggg cttgagtatg tcgatatctt
    37441 ttactctcat cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg cgctggctca
    37501 tgcggtacaa agcggtaagg cgctgtatgt cgggatctcc tcttactcgc cagagcggac
    37561 gcaaaaaatg gtcgagttgc tgcacgagtg gaaaattccg cttttaattc atcaaccttc
    37621 gtacaattta ctgaaccgct gggtggataa aagcggcctg ctggataccc tgcaaaataa
    37681 cggcgtgggc tgtattgcct ttactcctct ggctcaggga ttgctgaccg gaaaatatct
    37741 caacggcatt ccgcaagatt cacggatgca tcgtgaaggg aataaaattc gtggtctgac
    37801 gccgaaaatg cttaccgaag ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca
    37861 gcagcgtgga caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt
    37921 gacgtcggta ttgattggtg ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct
    37981 gaataatctg acatttagca ccgaggagct ggcgcagatt gatcagcata tcgccgatgg
    38041 cgaactgaat ctgtggcagg cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa
    38101 ccgcgcccga atcagatcag tgattatgac gagtcagtcg gtcaagatag cccatcacaa
    38161 atgcagaaag cacaaacgtc agatggataa tgacgtacca catcagtttg ttatccggga
    38221 catttttcgc atccataaag acgcgcagta agtgaatgga agaaattgcc acaatcgacg
    38281 ctgctacttt gtttttcagc gacgttgcgt ccattttccc cagccagttc agcttctctt
    38341 tgttctcgga gatatccagc tgtgagacga aattctcata accggaaaac atcaccatca
    38401 ccagcaaacc gccaaccagc gtcatatcca ccagcgacag caacacgagg atcaaatctg
    38461 attccgccat cgagaagata ttcggcagta cgtgaataat ctcctggaag aacttcagcg
    38521 ccagggcaac taacgccagc gaaaggccaa agtacacggg ggcaagcagc cagcgagaag
    38581 catacattgc attttcaaga aaacgttcca tacattcctg tcgtgtagtt aaaacgggca
    38641 acagtatatc gcaattgagt aaacagcacg caacagcaaa tcaacatata cgttaaacct
    38701 ttgtcgcata attctgaggt ttagggatct tcctggttcc cttatcaacg catcgagagc
    38761 gtctaccctt aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc
    38821 cgaccacgca gtattacact ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc
    38881 aggcgaagat gacaccgata ccggattgcg gcgagaaaac ctatgttggt agcgctcgcc
    38941 tgaaagatcg taaagcactg gtgacagggg gcgattccgg aataggtcgc gctgccgcca
    39001 tcgcttacgc gcgagaaggg gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag
    39061 acgctcagga tgtgaaaaag atcattgaag aatgcggacg caaagccgtt ctgctgccag
    39121 gcgatttaag cgatgagaaa ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag
    39181 gcgggctgga tattatggcg ctggtcgccg ggaaacaggt tgccattccg gatattgcag
    39241 acctcaccag cgaacagttt caaaagacct ttgccattaa cgttttcgcg ctgttctggc
    39301 taacccagga agcgatcccc ctgctaccga aaggtgcaag tatcatcacc acttcgtcaa
    39361 tccaggcata ccagccaagt ccgcatttac tggactatgc ggctacgaag gcggcgattc
    39421 tgaactacag ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg
    39481 tcgcgccagg ccctatctgg acggcactgc aaatttccgg cggacaaacg caggataaga
    39541 tcccgcagtt tggtcagaaa acgccgatga agcgtgcggg gcaaccggcg gaactggccc
    39601 ctgtatatgt ttatctggca agtcaggagt cgagctacgt caccgcagaa gtgcacggcg
    39661 tgtgcggcgg cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc
    39721 attgtgaatt cactcttacc tgatgtggcg aaaacgcctt atccgcctac aaacgcatga
    39781 agattcaaca tgttgcaata gattttgtag gcctgataag cgtagcgcat caggcattga
    39841 gcacctaatg ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca cgaagattca
    39901 acatgttgca atagattttg taggcctgat gagcgtagcg tatcaggcaa ttctacttta
    39961 cttcgctttg gcggtttctt cgccgaccag acctatcttc aggtaacccg cctgatgcag
    40021 cgtatccatt accttcatca acgtctcgta atcgacggtt ttgtccgctc ggaagaagat
    40081 ggtggtgtct ttcttgcctt cggttaacgc attcaacgcc gtaatcattg tttcatcggt
    40141 gaccggatcg ttaccgataa acatcgagtt gtctgccttc accgacagat aaaccggttt
    40201 ttccgggcgc ggctgcggcg tgctggtaga agcaggcaag ttcaccttca catctaccgt
    40261 cgctaacggt gccgccacca taaagataat cagcagaacc aacatcacgt cgataaacgg
    40321 cgtcacgttg atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc
    40381 catcggacat tatcctgcgc gtaatttttg tgcgacacga accggatgcg cagcggcgct
    40441 ggcttccaga tccaggtcac ggctttgcag caacaatacc tgcgctgcaa catcacccag
    40501 catcgcttta aagccgccaa tctggcgtgc aaatacgtta tagataacga ccgcaggaat
    40561 cgctgccacg aggccgattg ccgttgctaa cagagcttct gcgatacccg gcgcaacgac
    40621 tgccaggtta gtggtctgcg tttgcgcgat accaataaag ctgttcatga tgccccagac
    40681 cgtaccaaac agaccaacga acggagaaat cgcgccgatg gttgccagat agccgttacc
    40741 gcggcccatt tgacgaccca ctgcggcgac ccgacgctcc agacggaagc tggtacgttc
    40801 tttaatacct tcgttatcgt cgctgccttc tgacagttcc agctcattct gcgcttcatt
    40861 gagcaaatgc aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt
    40921 taaggaacgt gcttcagcca gcagttgctg ctcgcgctta aggcgacgct tctgattgaa
    40981 gaactctacg ctcttactga agaagattgc ccaggtgact acggaggcca aaataagccc
    41041 aatcatcacg cacttaacga caatatcggc gtgctgatac ataccccaga cggaaaggtc
    41101 cgtctgcatt aaattattac ccactgtgta tctccaggac gcaagtcaca aaatctgcgc
    41161 ataataatat caaaacgacg tcgaattgat agtcgttctc attactattt gcatgctgcc
    41221 gtacctttgc tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg
    41281 cgaaaatttt ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt catgctagtt
    41341 tagacatcca gacgtataaa aacaggaatc ccgacatggc ggacaaaaag cttgatactc
    41401 aactggtgaa tgcaggacgc agcaaaaaat acactcttgg cgcggtaaat agcgtgattc
    41461 agcgcgcttc ttcgctggta tttgagagta tggaagccaa aaagcacgcg acgcgcaatc
    41521 gcgccaatgg cgagttgttc tatggacggc gcggaacgtt aacccatttt tccttacaac
    41581 aagcgatgtg tgaactggaa ggtggcgcag gctgcgcgct atttccctgc ggggcggcag
    41641 cggttgctaa ttcgattctt gcttttgtcg aacagggcga tcatgtgttg atgaccaaca
    41701 ccgcctatga accgagtcag gatttctgta gcaaaatcct cagcaaactg ggcgtaacga
    41761 catcgtggtt tgatccgctg attggtgccg atatcgttaa gcatctgcag ccaaacacta
    41821 aaatcgtgtt tctggaatcg ccgggttcca tcactatgga agtccacgac gttccggcga
    41881 ttgttgccgc cgtgcgcagt gtggtgccgg atgccatcat tatgatcgac aacacctggg
    41941 cagccggtgt gctgtttaag gcgctggatt ttggcatcga tgtttctatt caggccgcca
    42001 ccaaatatct ggttgggcat tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt
    42061 gctgggagca gctacgggaa aatgcctatc tgatgggcca gatggtcgat gccgataccg
    42121 cctatataac cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa
    42181 gcagtctgaa agtggccgaa tggctggcag aacatccgca agttgtgcga gttaaccacc
    42241 ctgccctgcc tggcagtaaa ggacacgaat tctggaaacg agactttaca ggcagcagcg
    42301 ggctattttc ctttgtgctt aagaaaaaac tcaatgatga agagctggcg aactatctgg
    42361 ataacttcag tttattcagc atggcctact cgtggggcgg gtatgaatcg ttgatcctgg
    42421 caaatcaacc agaacatatc gccgccattc gcccacaagg cgagatcgat tttagcggga
    42481 ccttgattcg cctgcatatt ggtctggaag atgtcgacga tctgattgcc gatctggacg
    42541 ccggttttgc acggattgta taacattgcc acttttggac aattttgcag acattttatt
    42601 gtgaaaagtc ttaaattgtt gcgtccggga tcaaggcgtc ccggacgatt caggagtaca
    42661 ataggcagat aaaggcttaa acgctgttcc acaggaaagt ccatggctgt tattcaagat
    42721 atcatcgctg cgctctggca acacgacttt gccgcgctgg cggatcctca tattgttagc
    42781 gttgtttact ttgtcatgtt tgccacgctg tttttagaaa acggcctgct gcccgcctca
    42841 tttttgccag gcgacagctt gttgatactg gcaggcgcat tgattgccca aggggttatg
    42901 gattttctgc ctacgattgc gattctgacc gccgcagcaa gtctgggctg ctggctaagt
    42961 tatattcagg ggcgctggtt agggaatacc aaaacggtga aaggctggct ggcacagctt
    43021 cctgctaaat atcaccagcg cgccacctgc atgtttgacc gccacggtct gctggcgctg
    43081 ctggctggac gttttcttgc atttgtccgt acgctgctgc caaccatggc gggaatttcc
    43141 ggtctgccaa accgccgctt ccagtttttc aactggttaa gtggattgct gtgggtcagc
    43201 gtggtaacca gttttggcta tgccttaagt atgattccgt tcgttaaacg ccatgaagat
    43261 caggtaatga cgttcctgat gatcctgcca attgccttgt taaccgctgg cttgttaggc
    43321 acgctgtttg tggtgattaa aaaaaaatac tgtaacgcct gacgattttc cccgttcccg
    43381 gctgctgtac cgggaacgta tttaattccc ctgcattgcc cgcattcttg ccgcatcttc
    43441 ccccggcgtc acaccgaagt aacgtttaaa ctcacggcta aattgcgatg cgctttcata
    43501 gccgacgcgc atcgctgctg cgctggcctt catgccgtca tggatgatca tcatccgcgc
    43561 cttatgcaga cggtaattct tcaaatactg caacggcgag gtgctggtga cagacttaaa
    43621 attatggtgg aacgccgata cgctcatgtt ggcttctgcc gccagttgct cgacgctcag
    43681 gttttcggtg tatttattct caattcgttt cagcacgcgg ctaatcagac tgaagtgagt
    43741 ctggcgactg accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag
    43801 aatttcgcgg atgatctgtt tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc
    43861 gagtaaccgc tccgccgcga ataaaatctc ttctgataac gtggcggagt taatcccgct
    43921 ggctgccatc gacggctgga aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa
    43981 ctgcaaaata tcgacattga gacgcaaccc tgccagcggc acctctgacg tggcataggt
    44041 ttcgcactca aacggcaacg gcaccgtcag cagcaggtat tcattggcat cataacgaaa
    44101 cacgcg
//
LOCUS       DADLXX010000018        43840 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLXX010000018 DADLXX010000000
VERSION     DADLXX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.018"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1196)
                     /locus_tag="JNX27_000992"
                     /pseudo
     CDS             complement(1..1196)
                     /locus_tag="JNX27_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            1590..2960
                     /locus_tag="JNX27_000993"
     CDS             1590..2960
                     /locus_tag="JNX27_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7882285.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAITAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3142..5296
                     /locus_tag="JNX27_000994"
                     /pseudo
     CDS             3142..5296
                     /locus_tag="JNX27_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5349..7079
                     /locus_tag="JNX27_000995"
     CDS             5349..7079
                     /locus_tag="JNX27_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882286.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQYVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(7139..7630)
                     /gene="yajQ"
                     /locus_tag="JNX27_000996"
     CDS             complement(7139..7630)
                     /gene="yajQ"
                     /locus_tag="JNX27_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7882287.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7798..8709
                     /gene="panE"
                     /locus_tag="JNX27_000997"
     CDS             7798..8709
                     /gene="panE"
                     /locus_tag="JNX27_000997"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7882288.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8672..9262
                     /gene="yajL"
                     /locus_tag="JNX27_000998"
     CDS             8672..9262
                     /gene="yajL"
                     /locus_tag="JNX27_000998"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7882289.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDAQVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(9316..10764)
                     /gene="thiI"
                     /locus_tag="JNX27_000999"
     CDS             complement(9316..10764)
                     /gene="thiI"
                     /locus_tag="JNX27_000999"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7882290.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10970..11212
                     /gene="xseB"
                     /locus_tag="JNX27_001000"
     CDS             10970..11212
                     /gene="xseB"
                     /locus_tag="JNX27_001000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7882291.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            11212..12111
                     /gene="ispA"
                     /locus_tag="JNX27_001001"
     CDS             11212..12111
                     /gene="ispA"
                     /locus_tag="JNX27_001001"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7882292.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            12136..13998
                     /gene="dxs"
                     /locus_tag="JNX27_001002"
     CDS             12136..13998
                     /gene="dxs"
                     /locus_tag="JNX27_001002"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7882293.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTICVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            14053..15027
                     /gene="yajO"
                     /locus_tag="JNX27_001003"
     CDS             14053..15027
                     /gene="yajO"
                     /locus_tag="JNX27_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7882294.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDDVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(15081..15599)
                     /gene="pgpA"
                     /locus_tag="JNX27_001004"
     CDS             complement(15081..15599)
                     /gene="pgpA"
                     /locus_tag="JNX27_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7882295.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15577..16554)
                     /gene="thiL"
                     /locus_tag="JNX27_001005"
     CDS             complement(15577..16554)
                     /gene="thiL"
                     /locus_tag="JNX27_001005"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7882296.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(16632..17051)
                     /gene="nusB"
                     /locus_tag="JNX27_001006"
     CDS             complement(16632..17051)
                     /gene="nusB"
                     /locus_tag="JNX27_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7882297.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(17071..17541)
                     /gene="ribE"
                     /locus_tag="JNX27_001007"
     CDS             complement(17071..17541)
                     /gene="ribE"
                     /locus_tag="JNX27_001007"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7882298.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17633..18733)
                     /gene="ribD"
                     /locus_tag="JNX27_001008"
                     /pseudo
     CDS             complement(17633..18733)
                     /gene="ribD"
                     /locus_tag="JNX27_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(18737..19186)
                     /gene="nrdR"
                     /locus_tag="JNX27_001009"
     CDS             complement(18737..19186)
                     /gene="nrdR"
                     /locus_tag="JNX27_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7882299.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            19337..19876
                     /locus_tag="JNX27_001010"
     CDS             19337..19876
                     /locus_tag="JNX27_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7882300.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            20175..21059
                     /locus_tag="JNX27_001011"
     CDS             20175..21059
                     /locus_tag="JNX27_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7882301.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKIRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21236..21583)
                     /locus_tag="JNX27_001012"
     CDS             complement(21236..21583)
                     /locus_tag="JNX27_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7882302.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21712..22683)
                     /gene="secF"
                     /locus_tag="JNX27_001013"
     CDS             complement(21712..22683)
                     /gene="secF"
                     /locus_tag="JNX27_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7882303.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22694..24541)
                     /gene="secD"
                     /locus_tag="JNX27_001014"
     CDS             complement(22694..24541)
                     /gene="secD"
                     /locus_tag="JNX27_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7882304.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24569..24901)
                     /gene="yajC"
                     /locus_tag="JNX27_001015"
     CDS             complement(24569..24901)
                     /gene="yajC"
                     /locus_tag="JNX27_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7882305.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24924..26051)
                     /gene="tgt"
                     /locus_tag="JNX27_001016"
     CDS             complement(24924..26051)
                     /gene="tgt"
                     /locus_tag="JNX27_001016"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7882306.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(26106..27176)
                     /gene="queA"
                     /locus_tag="JNX27_001017"
     CDS             complement(26106..27176)
                     /gene="queA"
                     /locus_tag="JNX27_001017"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7882307.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            27269..27842
                     /gene="acpH"
                     /locus_tag="JNX27_001018"
                     /pseudo
     CDS             27269..27842
                     /gene="acpH"
                     /locus_tag="JNX27_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(27847..29664)
                     /gene="malZ"
                     /locus_tag="JNX27_001019"
     CDS             complement(27847..29664)
                     /gene="malZ"
                     /locus_tag="JNX27_001019"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7882308.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29820..31193)
                     /gene="proY"
                     /locus_tag="JNX27_001020"
     CDS             complement(29820..31193)
                     /gene="proY"
                     /locus_tag="JNX27_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7882309.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKIAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(31269..32588)
                     /gene="brnQ"
                     /locus_tag="JNX27_001021"
     CDS             complement(31269..32588)
                     /gene="brnQ"
                     /locus_tag="JNX27_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7882310.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32995..34290)
                     /gene="phoR"
                     /locus_tag="JNX27_001022"
     CDS             complement(32995..34290)
                     /gene="phoR"
                     /locus_tag="JNX27_001022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7882311.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(34348..35037)
                     /gene="phoB"
                     /locus_tag="JNX27_001023"
     CDS             complement(34348..35037)
                     /gene="phoB"
                     /locus_tag="JNX27_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7882312.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            35227..36429
                     /gene="sbcD"
                     /locus_tag="JNX27_001024"
     CDS             35227..36429
                     /gene="sbcD"
                     /locus_tag="JNX27_001024"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7882313.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            36426..39569
                     /gene="sbcC"
                     /locus_tag="JNX27_001025"
     CDS             36426..39569
                     /gene="sbcC"
                     /locus_tag="JNX27_001025"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7882314.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADMKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVELDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            39695..40876
                     /gene="araJ"
                     /locus_tag="JNX27_001026"
     CDS             39695..40876
                     /gene="araJ"
                     /locus_tag="JNX27_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7882315.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(41019..41927)
                     /gene="mak"
                     /locus_tag="JNX27_001027"
     CDS             complement(41019..41927)
                     /gene="mak"
                     /locus_tag="JNX27_001027"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7882316.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            42052..42963
                     /gene="rdgC"
                     /locus_tag="JNX27_001028"
     CDS             42052..42963
                     /gene="rdgC"
                     /locus_tag="JNX27_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7882317.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(43041..43496)
                     /locus_tag="JNX27_001029"
     CDS             complement(43041..43496)
                     /locus_tag="JNX27_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882318.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAAGRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(43493..>43840)
                     /locus_tag="JNX27_001030"
     CDS             complement(43493..>43840)
                     /locus_tag="JNX27_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY7882319.1"
                     /translation="LQVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPAS
                     LLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRK
                     IRSEARHRLEEKQ"
BASE COUNT        11416 a        11333 c        10669 g        10422 t
ORIGIN      
        1 ttcaatactg acatcgcata ttttggtttt ttcatttgcg ttaaagccat aggtcccgta
       61 atgttttaac gtgatgtaag gtaaagtatc accctgtgtt tctcgggttt tctgatcttg
      121 attacaacgg gttaaatatt catccttatt gaatttcatt gaatatttcc ccgcaccttt
      181 ttcgtaataa tcccatacct tatcacaatc aagcgtttca cctaatccgt tgtaatacat
      241 ccaacttaac cacaatgcgg cttttttgcc ccttgctgtt cagccttgtc aaaaagcatg
      301 ctcgctttag aataatcaac ctcaccggtc aaaccagaat agtatatatt ccccagggcc
      361 actaatgctt tttcgtcatt cttatttgca gctaattcgt accatttttt ggcttcgaca
      421 gcatcaatct ttaaaccagc actgccattt tcgtaggtat cgcccatggt atactgcgcc
      481 atcggataac ctttttctgc cgcagtacga aaccattgca gtgctttctt tatggtgtct
      541 aaagaacgtc tatcatcaga aggtgaaaaa tcatcgagat aaagatttcc gacaatataa
      601 tccatttcgg ggtcgccttc ctgcgcccat agagtcgcca gacgcagaat ttcaggttta
      661 ctgaaaccaa ttcgccccca aaaaagcaaa tcattctcgg gtgcctgacc gacataacga
      721 ctgacaaatt caattaaact cgcccgtttc gttttgtctg tctcagcccc ggatttcaat
      781 tcataggcct gattgattat tttattcatc aaatcaggcg gaatattaag atcgtcagtt
      841 ttcgacttac tggcatactt ctgtgctcgt gtcacatcta gggtgacgcc tttaccatat
      901 tgaaagaaat aacgtgcatt ataacaaccg tattcgcccc cctggttaca ggctttctca
      961 taccactgaa tcgcctttgg gatgtcaaca gatactgtca ctccgtgttc ataaataaat
     1021 cccagtagca ctagcgcctg cgtgttattg gtatcagcta attttttcag ttcttttaac
     1081 gccgtctgat ctttattgac aatacgttca tacagttgat caatatctgt tgaagcatga
     1141 caaaaagaag ccagagataa acccaatgat aataaaataa ctttaccaga ccacatacat
     1201 tcctccttga tatattcaac caccaaacat ccacctcatg aataaatacg ctgccataac
     1261 ggcccaggta ataagtaaac atttaagctg aaacagcata accagtgcct ccaacgcttc
     1321 tatttattgc gataaaataa caccatcctt tgataaaaag tcacacgccc atcaataaat
     1381 ccaaaaataa tgtcattagt tgcaggataa aatcatttga gcttttcatt tgttatattt
     1441 aacgcatgat gtattaatgg caagaatatg agagaaactc atacctgacg taaaaatcac
     1501 tctcttatgt aatatttact tattaacact ctgtttacct aacatgcgct ccgcactaca
     1561 ctatgtgggc aattttttaa gaggtggtaa tgaacgatta taaaatgacg ccaggtgagc
     1621 ggcgcgcgac ctggggttta gggaccgtat tttcattgcg catgctgggc atgtttattg
     1681 tactgccggt tctgactacg tacggtatgg cactgcaagg cgccagcgaa gcattaatcg
     1741 gtattgccat tggtatttat ggtctgaccc aggccgtttt tcagattccg tttggcctgc
     1801 tttccgaccg cattggtcga aaaccattaa ttgtcggtgg gctggcagtg tttgccgccg
     1861 gtagcgttat cgctgcgctt tctgactcca tctggggaat tattctgggc cgggcgctac
     1921 aaggctccgg tgcgattacc gctgccgtta tggcgctgct ttccgatctc acgcgcgaac
     1981 aaaaccgtac caaagcaatg gcgtttatcg gcgtgagctt tggcattacc tttgccattg
     2041 cgatggtgct tggcccgatc atcactcaca aacttgggct gcacgcgctg ttctggatga
     2101 tcgctattct ggcaacgacc ggcattgcgt tgaccatttg ggttgtgccc aacagtagca
     2161 ctcacgtact taatcgtgag tccggaatgg tgaaaggcag tttcagtaaa gtgctggcgg
     2221 aaccgcggct gctgaaactc aactttggca ttatgtgtct gcatattttg ctgatgtcga
     2281 cgtttgttgc cctgcccgga caactggctg atgcggggtt cccggcggct gaacactgga
     2341 aggtctatct ggcgacaatg ctaatcgcct ttggctcggt cgtgcctttc attatctacg
     2401 ctgaagttaa gcgcaaaatg aagcaagtct ttgtcttctg cgtcgggttg atcgtggttg
     2461 cggaaattgt gttgtggaac gcgcaaacgc agttctggca actggtggtc ggcgtgcagc
     2521 ttttctttgt ggcgtttaat ttgatggaag ccctgctgcc ctcacttatc agtaaagagt
     2581 cgccagcagg ttacaaaggt acggcgatgg gtgtttactc caccagccag tttcttggcg
     2641 tggcgattgg cggttcgctg ggcggctgga ttgacggcat gtttgacggt cagcgggtat
     2701 ttctcgctgg cgcaatgctg gccgcagtgt ggctggcagt cgccagtacc atgaaagaac
     2761 cgccgtatgt cagcagtttg cgcattgaaa tcccagaaaa cgtaatggca gatgaaaaac
     2821 tggaaaaatg cttactgaca acgcctggtg tcaaagatgt actgctggcg aagttagaac
     2881 attgcgtcta tgtgaaagta gacagtaaaa tcaccaatcg ttttgaaata gagcgagtaa
     2941 tcaacgaagt atcttattaa ataataagca cggtgctatt catgaaaaat aaaatagcac
     3001 cgtacttcgt aacaccataa tcctttaaac cctccattat taatacttcg cttaattcca
     3061 tataactgga cgattacatt agaagtaaac tatacttttt atgaaatatc agggataaat
     3121 tattcacatg gaaaatacat catgtacaaa tcattattta ctattatcat tctactgtat
     3181 agcgccagtt catatgcaaa aagcgaatcc cctcccgcaa gcactataac tttcattgca
     3241 caaatcgatc atgttgagct aaataaaaca gcattatctc atgatatgaa actcgttctt
     3301 gccgatcgtt tttatttcaa aaccagtaat ccttgcgaaa caaattcaat ttctgctcga
     3361 ccagaatcaa tggatattac tgacaaagcc tatgccaaca aattaaaaaa attagaaact
     3421 ccttttcttg atgatcgcta tttctttatg accatcaatc agtgtgacgg taatggcact
     3481 cccatgctga cagacataaa agtatgtaca acagaattat gtggtgctga atatacaaag
     3541 aaaatgtcta ctgtatggct cgatgaaact ttaaaaccaa ctgctaaacg tctggcaatt
     3601 tattttattc aatctccttt accctatgac gaggagaaaa aacaatggaa agtcaccgga
     3661 tggtatatct catcaactga taatcagtct tcaactaaac agattgcatt tgaaggatat
     3721 acagacacag ataacttctc cgattctaaa tttatttctt catttaaatc ctggtttgaa
     3781 agtggaaaga taaaaaacat tgctaactat aatccaaaag gagaaagaga tggaaaatac
     3841 gaaagttatt acaatagcaa tggtaacgtc tatgaatctc taagtttttc taatggtctt
     3901 atcaacggtg agtttattac atattatgaa aatggggcca ttgaaagtaa acgtcatttt
     3961 aataacgaca aaattaaaga tggtgaatgc cctcattttt atgaagatgg gaaaatcaaa
     4021 cagcaacata gctatctgaa tagtaaactc gatggtcctg catatgaata ttatcctgat
     4081 ggcaaactca tgcaggaaaa tttctatcag caaagtgtgt tgattggaaa cgataccagt
     4141 tactaccaat ccggaaaaat aagctctata cacaatagaa attcccgtgg tcagtatgat
     4201 ggagctaacg aacgttatag tgaagagggt aagttacagt ctaaatccgt ctataaagac
     4261 ggaaaacaaa tttctgttca aacctggtat gaaaatgggc agaaagaaga ggagaaacat
     4321 tttgatgagc aaggccaatt aaatagtctg gttaagcaat ggtataaaaa tggtaatctt
     4381 gcaaaatcac aaaactataa acatgacata ctcgatggta acagtgaaga gtggtacgaa
     4441 aatggcattc ctgaaagttt gtacccttat aagaatggaa aaaccgacgg cattgccaaa
     4501 tcatggaata agtatggtaa attaacctat tcaatcgaat acaaaaatgg cgtagagaat
     4561 ggggcatatc gtaactggag caaaaacaca ggtaaactaa ccaaagaaac cctgtacgtc
     4621 aatggtatca gacagggagt ggaaaaagag tttaacgatc gcacaggtaa actattaacc
     4681 tccacgcaat atgttaataa taaaagacat ggtacggagg aaacttatga tcaaaacgga
     4741 ataaaatata ttacatgcta tcaaaatgat cagaaattat cgtctttaga caatccaaca
     4801 caaataaaag ataatgcaac aacaggcgat tccagtgctc aatttgcatt agggaaatac
     4861 gaattcatct gtgcaaatat agatgaaggg attaaatggt taaccaaatc tgcagagcaa
     4921 aaaaaacact gatgcgatat actttttagc tacagcctac aaaggcaatg gtatacctgc
     4981 aaacaatgaa aaatacctag catatctgca acaagctgca acactgggca gtagtaatgc
     5041 ccaggcggaa attggctatt tatatctcat cggaaaagaa ctaccacaga atcttcctga
     5101 tgctggtgtc tggttcaaaa aagcagctgc acaaggtaat tttgccgcac atttctatct
     5161 gggaaggatg tatcagaatg gtgacggcgt tgagagaaat atggaaaaag ccaggtttca
     5221 tctatctaat gcggcagaag gtggaataga acctgcgctt aaagcattaa atgaacttga
     5281 acatcaaaca aaataaagcg ctcagggacc acgtaaggtt catcaaacag aaaaggatat
     5341 agtttgcgat gaaagcgaaa tctactttgt tattaatgat tttatctgtt ttttattctc
     5401 atcaggtttt atccagcgac cccactcagc cggatgttta ttctgtcgtc gagaaaaaac
     5461 taaactctgc tttacctctg gaagaaaata ccaccttcag atcgcaggct gaatggttta
     5521 atttgcaata tgaattatta gtatctggct accctgaacg tgcttacaat ttactgagca
     5581 aacttgagac agaatcgaaa aacgctaaat attatctgga tttaactgcc tccagagtcg
     5641 ctgaagaaga agcaccagag acagccttaa cttttttatc aaaaattggg ttaaataaac
     5701 cggctactct tacatccttt gagagctata tcaatagctg gataaaaagc aaacaacccg
     5761 aaaaggctat gcagcttctc agcctcgatg agaatgccct gaattattat ttaccgactg
     5821 tgttagtcgc atatcagatc gttcctgata aggcaataag tatttataat tctgtatatg
     5881 gagataatgt tgttataccc tatcagcaag tcaccatgtt acttacagtt gcggagggtt
     5941 atcaaacaaa aggcgatata aaaaacgcca ttcattatgc cgataaggcg ctgaatatgt
     6001 ttgataaagc tattgctgta gatagcagtc gtgaatccca ttatagtgat gagtatttga
     6061 aactcatgaa tatctacgcc gccaatggca acaaagaaaa agcggtggtt ttatctcaac
     6121 gtctacacaa agccattacc gaatctcaat cgaattactt aagtactctg gaaggaattt
     6181 tattgtttta tagaaataat gatatgcagc aagactatca aaacagcctt tcaaattaca
     6241 tagtatttat cgatgaaatt tttgcctttt ccccatcaac aagaagtgaa ttaagcctca
     6301 ttaatttatt aaattcatta aatgaggtgg aattgatgag aaaacgtctg gcgtttctga
     6361 tgacagcacc agaatacgct tgttatgata gtcagtattg ttatgaagac aaagtcagcg
     6421 cattaaaaat cctgtatcgt catcatgaca atgagctaat ggacaaatat ttcacgactc
     6481 ttcttaaaga gatcagtaat caaccattag aagactggga tataataata agtaatactg
     6541 gtgaaaaatt tagtgaagtg ggtctaacca aatatgctca acatctcgca tctttcgctg
     6601 aagaatttta tataaaagag caaaaatcac attctgaaag tgaaatgaga ggactatttt
     6661 catcacttgc cgaattatat agttttggta atgatactgc cagcgcaaac cgagtgttcc
     6721 atcagtatgt ccctgccttc aatacagatc atatgattca atattttata aatgccaaag
     6781 aatggaacaa tgccagggaa ttattgataa aagaggaaat gttgggtatg cataatctga
     6841 tattgttgga gaatatttgt atgcaaaaaa atgcagaatg catggcacac attaccttta
     6901 cgcttaataa attaacaaca caaccagcaa taacaaaaat agatagcgtg ggtaatgagc
     6961 aactttatca gattggcaaa atctaccata agttggaaat taaacctgag ccagagcaac
     7021 aggttttaat tcagtcgctt tatgaccggg catcaggaag cgcctctgca actcaataaa
     7081 tgcatggtca aaagggccac cacggtggcc ctgacaatca gtagctatat ccagaaagtt
     7141 aatcgcggaa gtttttgaac tggaacggct gaccgagatc gccaccgcgc accatcgcca
     7201 tgacagcctg caaatcatca cgagatttgc cagtcacgcg gatctcatcg ccctgaattt
     7261 gcgcctgcac tttcagtttg ctgtctttga tcatcttgac gatttttttc tgagtggcgc
     7321 tctcaatgcc ctgtttcagt ttcgcttcca caaaccaggt tttaccgcta tgaacgatat
     7381 tttccggtac atccagtgaa ctgccttcaa tgccgcgctt cagcagcttg gcacgcagaa
     7441 tatccagcaa ctgattgacc tggaagtcgg actcgctcaa cactttgatg gttttgctgg
     7501 cgtcgttcag ctcaaatgag gcttcaacgt tacggaagtc aaaacgggac tccacttcgc
     7561 gactcgcgtt atcgaccgcg ttacgtgctt cctgaagatc aacttcagag acaatatcga
     7621 aagatggcat cttttcctct cccttcattt ttgatgcgaa gcataatacc tgcaaagtta
     7681 aataaccaac agtcctgcac caggacgtat cccttaattg tgcatagtcg caacagcatg
     7741 cacaattaag ggatagcctt attgaacacc cggagtggtt gtgggtgagg aggaacaatg
     7801 aaaattaccg tattgggatg cggtgcctta gggcaattat ggcttacagc actttgtaaa
     7861 cagggtcatg aagttcaggg ctggctgcgc gtaccgcaac cttattgtag tgtgaatctg
     7921 gttgagacag atggttcgat atttaacgaa tcgctgaccg ccaacgatcc cgattttctc
     7981 gccaccagcg atctgctcct ggtgacgctg aaagcatggc aggtttccga tgccgtcaaa
     8041 agcctcgcat ccacactgcc tgtaactacg ccaatactgt taattcacaa cggcatgggc
     8101 accatcgaag aattgcaaaa cattcagcag ccattactga tgggcaccac cacccatgcc
     8161 gcccgccgcg acggcaatgt cattattcat gtggcaaacg gaatcacgca tatcggtccg
     8221 gcacggcaac aggatggcga ttacagttat ctggcggata ttttgcaaac cgtgttgcct
     8281 gacgttgcct ggcataacaa tattcgcgcc gagctgtggc gcaagctggc cgtcaactgc
     8341 gtgattaatc cactgactgc catctggaat tgcccgaacg gtgaattacg tcatcatccg
     8401 caagaaatta tgcaaatatg cgaagaagtc gcggcagtga tcgaacgcga agggcatcat
     8461 acttcagcgg aagatttgcg tgattacgtg atgcaggtga ttgatgccac agcggaaaat
     8521 atctcgtcga tgttgcagga tatccgcgcg ctgcgccaca ccgaaatcga ctatatcaat
     8581 ggttttctct tacgccgcgc ccgcacgcat gggattgccg taccggaaaa cacccgcctg
     8641 tttgaaatgg taaaaagaaa ggagagtgaa tatgagcgca tcggcactgg tttgcctcgc
     8701 ccctggtagt gaagagactg aagccgtcac cacgatcgac ctcttggttc gcggcggtat
     8761 caaagtcacc actgccagcg tcgacagcga tggcaacctg gcaattacct gctcgcgcgg
     8821 cgtgaagctg ctggcggatg cgccgctggt cgaagtggct gatggcgaat atgacgtgat
     8881 cgtgctgcct ggtggcatta aaggcacgga gtgttttcgc gatagcactc tgctggttga
     8941 aaccgttaaa cagttccacc gttccgggcg tatcgtcgcg gctatttgcg ccgcgccagc
     9001 caccgtgctg gtgccgcacg atatctttcc gattggcaat atgaccggct tcccgacgct
     9061 gaaagacaaa attcccgctg aacaatggca ggacaagcgc gtcgtctggg atgcacaggt
     9121 aaaattgctg accagccagg ggccgggtac agctatcgac tttggtctga aaattatcga
     9181 cctgctggtt gggcgcgaga aagcccatga agtggcatca caactggtga tggcggcagg
     9241 gatttataat tattacgagt agtgtcggat gcggcaaacg tcgcatctga ccagatgcga
     9301 cgtaaaaact acaaattacg ggcgatatac cttcacattg ttaaagcctt gctcgcgcag
     9361 atagagcgcc tgcagacggc tcatcacccc gcgctcacac cacagcagcc aggttttgtt
     9421 ctggtcgaga tcgccaaatt tggtgctcag tttatagaac ggcagagaaa ccacatcaat
     9481 cccttcgact ttcagtggct tatcttcctg ttcatcgaca gaacggatat cgagaatcac
     9541 gtcgttcggg ccgaagccgt tgacggtttc cacttccacc acttcctgct cggtctgctg
     9601 ggcgatttcg cggatatcaa cgttattcgc ttcctcaacc actttatcga gaatactgaa
     9661 gtcgaacttc tcttcttccg cttcaatctt cgatttaacc gctttcaccg tcgggctttt
     9721 ggagatcacg ccacaatatt ccggcatcgt gcgagcaaag tcttcggtgc caatctggcg
     9781 agccaggttg atgatgtgct ctttgtcgta agaaatcagc ggacgcagga tcagcgtgtc
     9841 agagacgtta tcaatcaggc gcaggttggt cagcgtctga ctggacacct ggccgagcgc
     9901 ttcgccggtg accagcgcct gtacgccgta acgttcagcc actttagatg cggcacgcac
     9961 catcatacgt ttgaggataa cgcccatctg accgtcgtcg attttctcga gaatttcccc
    10021 gacaaccggt tcgaaattaa tggcgacaaa acgcacgcgg tgggagctgc caaagcggtt
    10081 ccacagataa tgcgccacct gacgcacgcc aatttcatgc gctgcgccgc cgaggttaaa
    10141 gaagcagtaa tgcacgcggc agccgcgacg catcaacata taactggaaa caccggagtc
    10201 gaaaccaccg gaaatgagcg acagcacatc ttcctgggtg ccaatcggga aaccaccaat
    10261 accttcgtag cgacctttaa tcagcaggag gcgatcgtct tccacttcca gatgaacagt
    10321 tacatccggg ttagtcagct tcacgcgcgc ggattcaata tgctgattta aaccgccgcc
    10381 gacgtaacgt tccacatcaa tcgagctaaa atcatgtttg ccacggcgct tcacgcgtac
    10441 gcagaaggtt ttgccttcca gctgatcgcg atactgaacc aacgctttct caaaaatatc
    10501 gtgcatgtcg gtaaacggca cgtcttcgac ttcgagaata tggtggatac ccggaatacg
    10561 ggtcagggcg tcgcgaatag tcagacgctg gttttcatct ttcgcgcgaa cttcgatgtt
    10621 atcccagtgg cggacgacag cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt
    10681 cccggtaagg atttttataa agcgcaagcg cacagattgg cttttgatgg tgatttccgg
    10741 gaacaattta atgataaact tcatggcggc aatggttcgt tggcaagcct taagcgactt
    10801 gtatagggaa aaatacagca gcccacacct gcggctgcat ccaggcgcgg aagtatacca
    10861 ctaacatcgc tttgctgcgc acatcacctt accattgcgc gttatttgct atttgccctg
    10921 agtccgttac catgacgggg cgaaaaatat tgagagtcag acattcatta tgccgaagaa
    10981 aaatgaggcg cccgccagct ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg
    11041 tctggaaagt ggcgacctgc cgctggaaga ggcgctgaac gagttcgaac gcggcgtgca
    11101 gctggcacgt caggggcagg ccaaattaca acaagccgaa cagcgcgtac aaattctgct
    11161 gtctgacaat gaagacgcct ctctaacccc ttttacaccg gacaatgagt aatggacttt
    11221 ccgcagcaat tcgaagcctg cgttaagcag gccaaccagg cgctgagccg ttttatcgcc
    11281 ccactgccct ttcagaacac tcccgtggtc gaaaccatgc agtatggcgc attattaggt
    11341 ggtaagcgcc tgcgaccttt cctggtttat gccaccggtc atatgtttgg cgttagcaca
    11401 aacacgctgg acgcacccgc tgctgccgta gagtgtatcc acgcttactc attaattcat
    11461 gatgatttac cggcgatgga tgatgacgat ctgcgtcgcg gtttgccgac ctgccatgtg
    11521 aagtttggcg aagcaaacgc gattctcgct ggcgacgctt tacaaacgct ggcgttctcg
    11581 attctaagcg atgccaatat gccggaagtg tcggatcgcg acagaatttc gatgatttct
    11641 gaactggcga gcgccagcgg tattgccgga atgtgcggtg gtcaggcatt agatttagac
    11701 gcggaaggca aacacgtacc tctggacgcg cttgagcgta ttcatcgtca taaaaccggc
    11761 gcattgattc gcgccgccgt tcgccttggt gcattaagcg ccggagataa agggcgtcgt
    11821 gctctgccag tactcgacaa gtacgcagaa agcatcggcc ttgccttcca ggttcaggat
    11881 gacatcctgg atgtggtagg agatactgca acgttgggaa aacgccaggg tgccgaccag
    11941 caacttggta aaagtaccta ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc
    12001 cgggatctga tcgacgatgc ccgtcagtcg ctgaaacaac tggctgaaca gtcactcgat
    12061 acctcggcac tggaagcgct agcggactac atcatccagc gtaataaata aacaataagt
    12121 attaataggc ccctgatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac
    12181 tccacccagg agttacgact gttgccgaaa gagagtttac cgaaactctg cgacgaactg
    12241 cgccgctatt tactcgacag cgtgagccgt tccagcgggc acttcgcctc cgggctgggc
    12301 acggtcgaac tgaccgtggc gctgcactat gtctacaaca ccccatttga ccaattgatt
    12361 tgggatgtgg gacatcaggc ttatccgcat aaaattttga ccggacgccg cgacaaaatc
    12421 ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt ggcgcggcga aagcgaatat
    12481 gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg ccggaattgg tattgcggtt
    12541 gctgccgaga aagaaggcaa aaatcgccgc accatctgtg tgattggcga cggcgcgatt
    12601 accgctggca tggcgtttga agcgatgaat cacgcgggcg atattcgtcc tgatatgctg
    12661 gtggtcctca acgacaatga aatgtcgatt tccgaaaatg ttggcgcgct taacaaccat
    12721 ctggctcagc tgctttccgg taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt
    12781 ttctctggcg tgccgcccat taaagagctg ctcaaacgta ccgaagaaca tattaaaggc
    12841 atggtggtgc ctggcacgtt gtttgaagag ctgggattta actacatcgg cccggtggac
    12901 ggtcacgatg tgctggggct tatcaccacg ctaaagaaca tgcgcgacct gaaaggcccg
    12961 cagttcctgc atatcatgac caaaaaaggt cgtggttatg aaccggcaga aaaagacccg
    13021 atcactttcc acgccgtgcc taaatttgat ccctccagcg gttgtttgcc gaaaagtagc
    13081 ggcggtttgc cgagctattc aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa
    13141 gacaacaagc tgatggcgat tactccggcg atgcgtgaag gttccggcat ggtcgagttt
    13201 tcacgtaaat tcccggatcg ttacttcgac gtggctattg ccgagcaaca cgcagtgacc
    13261 tttgccgccg gtctggcgat tggtggatac aaacccattg tcgcgatcta ctccaccttc
    13321 ctgcaacgcg cctatgatca ggtgctgcat gacgtggcaa ttcaaaagct accggtcctg
    13381 ttcgccattg accgcgcggg cattgttggt gctgacggtc aaactcacca gggcgctttt
    13441 gatctctctt acctgcgctg cataccagaa atggtcatta tgaccccgag cgatgaaaac
    13501 gaatgtcgcc agatgctcta taccggctat cactataacg atggcccgtc cgcggtgcgc
    13561 tacccgcgcg gcaacgcggt tggcgtggaa ctgacgccgc tggaaaaact accaattggc
    13621 aaaggcattg tgaagcgtcg tggcgagaaa ctggtgatcc ttaactttgg tacgctgatg
    13681 ccagaagcgg ccaaagtcgc cgaatcgctg aacgctacgc tggtcgatat gcgttttgtg
    13741 aaaccacttg atgaagcgtt aattctggaa atggccgcca gccatgaagc gctggtcacc
    13801 gtagaagaaa acgccattat gggcggcgca ggtagcggag tgaacgaagt gctgatggcc
    13861 catcgtaaac cagtacccgt gctgaacatt ggcctgccgg acttctttat tccgcaagga
    13921 actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg ccggtatgga agccaaaatc
    13981 aaggcctggc tggcataatc cctactccac tcctgctatg cttaagaaat tattcatggc
    14041 aggagtggca gcatgcaata caacccctta ggaaaaaccg accttcgcgt ttcccgactt
    14101 tgcctcggct gtatgacctt tggcgagcca gatcgcggta atcacgcatg gacactgccg
    14161 gaagagagca gccgtcccat aattaaacgc gcgctggaag gtggcataaa tttctttgat
    14221 accgccaata gctattccga tggtagcagc gaagagatcg ttggtcgcgc actgcgggat
    14281 ttcgcccgcc gtgaagacgt agtcgttgcc accaaagtgt tccatcgcgt tggtgattta
    14341 ccggaaggat tatcccgtgc acaaattttg cgctctatca acgacagcct gcgacgtctc
    14401 ggcatggatt atgtcgatat cctgcaaatt catcgcgggg attacaacac gccgatcgaa
    14461 gagacgctgg aagccctgaa cgacgtggta aaagccggga aagcgcgtta tatcggcgcg
    14521 tcatccatgc acgcttcgca gtttgctcag gcgctggaac tacaaaaaca gcactgctgg
    14581 gcgcagtttg tcagtatgca ggatcactat aatctgattt atcgcgaaga agagcgcgag
    14641 atgctgcctc tgtgttatca ggagggcgtg gcggtgattc cgtggagccc gatggcgcgg
    14701 gggcgactga cgcgtccatg gggagaaact accgcacgac tggtgtctga tgatgtgggg
    14761 aaaaatctct ataaagaaag cgatgaaaat gacgcgcaga tcgcagagcg gttaacgggc
    14821 gtcagtgaag aactcggtgc aacacgagca caagttgcgc tggcctggtt gttaagtaaa
    14881 ccgggcattg ccgcaccgat tatcggaact tcgcgggaag aacagcttga tgagctgctg
    14941 aacgcggtgg atatcacttt gaagccagaa cagattgccg agctggaaac gccgtataaa
    15001 ccgcatgcgg tcgtaggatt taaataagag tccaggcctg atgagacgtg acaagcgtca
    15061 catcaggcat cggtgcacaa ctacgacaga atacccagcg gccagtgatg accgataaag
    15121 tacaggatgc ctgcggaaat caccccggcg acaatatcgt cgatcatgat ccccatgccg
    15181 ccatgtacat tgcgatcaaa ccagcggatc ggccacggct tccacatatc cagaatacgg
    15241 aaaatcacaa acccggcggc aacccactgc cagtcattgg tcggcagcgc catgagcgtg
    15301 atccacatac caataaattc gtcccagaca atgctgccgt gatcgtgcac gcccatatct
    15361 ttcgccgttt gatgacaaag atagacgccg atacagatcc ccaacatcac caccagcgag
    15421 tagagctgcc agggtaaaaa ggtcatcaga taccagaaag ggatcgccgc cagcgatccc
    15481 atcgtgccag gaacgattgg gcttaatcca cttccgaatc cgacagcaag cagatgccac
    15541 ggattactca tcttcaggcg acttttcgcg acatctttat ggcgtgacaa aatggtcata
    15601 tcctttccag tcaaacgtga caggttcgcc gtcacgaata aaacaaagcc cttcgatatc
    15661 ggcggtcatt tgcccgatac aggtaaacgg tacgccaagg tgtccgagag caacatccag
    15721 cgcgccacgg ttcagttccg gcaccgtgaa acacaactcg taatcttcac cgccagagag
    15781 cgcccagcgc agcgcctgtt ccggttcaac atggcgagaa agcgcatcag aaaacggcag
    15841 caatgccagg tcaatacgtg cgccgcagtc gctggctttc acgatatgcc cgagatcgga
    15901 aatcagaccg tcagagagat cgatagctga atttgccaga tcgcgcagtg cctgcccctg
    15961 taaaatacgc ggcgatggac ggagatgacg tttgatcaag tagtcagcat ctttagcatc
    16021 ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg gcgctatcgc ccggtgtacc
    16081 ggtcacatag atccagtcac ccggtttcgc cccagagcgc gttaaggctc gtcccatcgg
    16141 aacaaagccg tggataccca acgtcattga taatggccca cgcgtggtat cgccgccaat
    16201 gagttgcata tcgtaataat tgagaagatc aaacaaactg tcgctgaagg actcaagcca
    16261 cgcttcgtct acgtccggta aggttaatgc cagcgtcagc caggccggat cggcccccat
    16321 cgctgccaga tcgcttaggt tcaccgccag tgctttataa gccagatcag caggatcgat
    16381 atcagggagg aaatggttgc ccgccaccag cgtatcagtg ctgatcgcca gggtctgttt
    16441 ctcggggata ttgagaagag cgcagtcgtc gccgatgccc agttcgacat caagacgaga
    16501 acttcttaca cggtcaaaat aacgggcaat cagggagaac tcgccacatg ccatacgtta
    16561 tgcctcagca ggtaaaaaag aaaaggccgg cgatcatgga acagtcttcc gtgaatctac
    16621 cggcctggat attacttttt gttagggcga atcacaggtg ctgctttatc gagtacgccg
    16681 ttgacgaact tatggctgtc ttctgcgccg aacgatttcg ccagttcgat cgcttcgtta
    16741 atggccactt tgtatggcac atcactacgt ttagacagtt cgtacagcgc aatgcgcagt
    16801 actgcttttt ctacctggcc cagttcttcc agcaggcggg acaggtaggg cttcatcagt
    16861 ccgtcgaggt atgcggtgtt agtcgccacc ccggccagca gctcacggaa gtacaggacg
    16921 tcaacgtctt ttacatcctg ttcagccagg aactggtatt caacatcagc gatgtcgttc
    16981 tgggacaact gccaggagta gagcgcctgg acggcacact cacgagcgcg gcgacgagca
    17041 gcaggtttca cggatttccc cttactaatt tcaggccttg atggctttca atacattaat
    17101 catttcaagc gcggtcagtg cagcttctgc acctttgttg ccagctttgg tgccagcacg
    17161 ttcgatcgct tgttcaatgc tttcagtggt cagaacccca aaagcaaccg gaatttcgct
    17221 gtcctgggca acatgcgcca ggccgttgct tgcaccacca gcgacatatt caaagtgggc
    17281 agtgccacca cgaataaccg tacccagcgc aatcaccgcg tcgtatttac cggttttagc
    17341 cagtgcaccc gccgccagcg gcagctcata ggcaccaggc acccaaacaa cggtaatgtt
    17401 ttcatctttt acctgaccga tacgtttcag tgcgtcaatt gcaccttcca gcaggctgtc
    17461 attgataaag ttgttgaaac gcgcgatggt gatggcgacg cgagcgtccg gggtagcaac
    17521 gttagcttca ataatgttca ttttcttcct tcgggttcga gtatggcccc gcaggggggc
    17581 ggattttagc ataatatttc gtgcgctgct tccctttcga gccgggagag catgccccca
    17641 ctaaatgcag gcaaacatcc gggcctacat gacgtatctc tttgaattta aattgggggg
    17701 cgtcggctaa tttctcaagc cctggcagcg agcataatcc acgggcgtcg ctgcctaata
    17761 gtttaggtgc gatatagaca atcagttcat cgactaaacc cgcctgtagc aatacgccag
    17821 cgagcgttgg ccccgcttcc acccagatgc tgttaatctg ctgtttaccc agttgcatca
    17881 tcagtacaac cagatccaga tgacctttat gctctggaat cagcaaggta cgcaccgttt
    17941 ccggccactc acgagaatct tcctgcgtac gcgcgaacca ggtttcgccg ggctgctgca
    18001 caatgcgatg ttccggcgtc acgcgatttt ggctatcaat cacaatacgt actggctggc
    18061 ggagattttg ttgcgggtag agcgcctgag tttgttcatc cagttcagac caacgcactg
    18121 ttaaggctgg atcatccgcc agcaccgtgg cgctgctggt taaaatggca tgactttgtg
    18181 cgcgctgtcg ttgtacgtcg cgccgcgcct gaggcgaagt gatccactgg ctttcgccgc
    18241 tcgccatcgc cgtgcggcca tcaagcgatg cgccaagttt taactgaata taaggaaagc
    18301 cggtgcgcat ccgcttgaga aagcctttat tcaattgctc ggcttcactc atcatcagcc
    18361 cgtggctgac gtcaatgcca gcctgttgca gacggtaaag tccacgccca gcgacctgcg
    18421 ggttaggatc ttgcatcgct gcgaccacgc gcgctacgcc agcggcgatg agtgcgtcac
    18481 agcacggtgg cgtacggcca tgatggctac agggttcgag tgtcacatac gccgttgcgc
    18541 ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc ggcatgtggt tcacccgctc
    18601 gttggtggta accttcaccg acaatttcgc catctttgac aatgacgcac ccgacattcg
    18661 ggttggggtg ggtggtaaaa cgtcctcgtt gcgccagctt tagcgcccgc gccatgtaat
    18721 actcgtcctg cacggcttag tcctccaggc gcgcgatctc ttcgccaaat tctttgatat
    18781 cttcgaaact gcggtagaca gaggcaaaac ggatataggc gactttatcg agctttttca
    18841 attgctccat caccagattg ccaatcatct tgctcggtac ttcgcgctca ccggtggcgc
    18901 gcagctgcga tttaatatga ttgattgcca tttcaacgta atcggaactc accggacgtt
    18961 tttccagcgc ccgcagcatt ccgctacgca atttctcttc attaaacggt tcacgcacgt
    19021 cgttgctttt tacaacacgc ggcataacca gctccgccac ttcaaaggtg gtgaaacgtt
    19081 cattacacac cagacactgc cgacggcggc gtacggatga accctcgccc acgagacgag
    19141 agtcaattac cttagtgtcc acggcgaaac agaatgggca atgcatacgg cgtcctgacc
    19201 aggtggttaa cggaaatcta ttttaccctg aactgacgat acaacaaagg cgcaacgctt
    19261 ttgagataaa ggatcgatgc tttcgctgct tttaccctct accattaggc taattgcgac
    19321 gagaaaggga cgacctatga atactaacgt ttttcgactg ctcctgctgg gaagcctgtt
    19381 cagccttagc gcctgtgtgc agcaaagtga agtgcgacag atgaaacaca gcgtcagcac
    19441 gctgaaccag gagatgacgc aactcaatca agaaacagtc aaaatcactc agcaaaacag
    19501 gctgaatgca aaatccagca gtggggttta ccttctgcct ggagcgaaaa caccggcaag
    19561 actggaaagc cagatcggta ctttacgtat gtcgctggtg aatattacgc ctgatacaga
    19621 tggcaccacc cttacactac gtattcaggg tgagtcgaat gatcccttgc ccgcattcag
    19681 cggaactgtt gaatatgggc aaattcaggg aacaatagac aactttcagg aaatcaatgt
    19741 gcagaatcaa ttgattaatg cccctgccag cgtcctcgcc cccagcgatg ttgatattcc
    19801 gttacaatta aaggggattt ctgtggatca gttaggcttt gtacgtatcc acgacattca
    19861 acctgttatg cagtaaacgt atttcgggac gattttgtgc ctcccgcaac atctttcccc
    19921 gtcattttgt tactctgctt acatcacttg gattgatagt aaaagtttgc aacaagggcg
    19981 aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga acgcaatcga ttacgtaaat
    20041 gatagaactg tgaaacgaaa catatttttg tgagcaatga tttttataat aggctcctct
    20101 gtatacgaaa tatttagaaa cgcaatttgc gcctttttca ctcccgcaag ggattttcaa
    20161 acagtggcat acatatgaaa aaaacattac tggcagccgg tgcggtactg gcgctctctt
    20221 cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca gtatctttcc gactggtggc
    20281 accagagcgt taacgttgtc ggaagctatc acacccgttt cggaccgcag atccgcaacg
    20341 atacctacct tgagtacgaa gcattcgcta aaaaagactg gttcgacttc tatggttatg
    20401 cggatgcgcc ggtattcttc ggcggtaact ccgatgctaa aggtatctgg aaccacggtt
    20461 ctccgctgtt tatggaaatc gaaccacgtt tctccatcga caagctgacc aatactgacc
    20521 ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa ctacatttac gacatgggtc
    20581 gtaataaaga tggtcgccag agcacctggt acatgggtct gggtaccgat atcgacactg
    20641 gcctgccgat gagcctgtcc atgaacgtct atgcgaaata ccagtggcag aactatggcg
    20701 cagcgaacga aaacgagtgg gacggttacc gtttcaaaat taaatacttt gtgccgatta
    20761 ccgatctgtg gggcggtcag ctgagctaca tcggcttcac caacttcgac tggggttccg
    20821 atttagggga tgacagcggt aacgcaatca acggtattaa gatccgtact aataactcta
    20881 tcgcttccag ccatattctg gctctgaact acgatcactg gcactactct gtcgtagctc
    20941 gttactggca cgacggtggt cagtggaacg acgatgcaga actgaacttc ggcaacggca
    21001 acttcaacgt tcgctctacc ggctggggtg gttacctggt agtaggttac aacttctgat
    21061 tatgaaaatg ccgggattta ttcccggcat ttctgattgt taacaacgtg tgctttgttc
    21121 atgccggatg cggcgtaaac gctttgtccg gcccacaaaa gcacgtaaat tcaatatatt
    21181 gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc aattttacgt tttaatcact
    21241 tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt gtacttcgct tcaccgacat
    21301 ctttctgcgc atcttcccct gcgataacgg tcgtaccata ctgatccgct tcggtatatt
    21361 tcgaatgctc atgatcgtgg caatagagac acaacaattc ccagttactg ccatcttccg
    21421 ggttattggt atggtcgtga tcaatgtggt gaacggtaag ttcacgcagg ttagaataaa
    21481 caaactcgcg ggaacagcga ccgcagaccc acggatagat ttttaatgct ttttcgcgat
    21541 agccgctttc taaccgtgca tagttttttg ggatgatagc catgaaaagt tacctggatg
    21601 aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt ttcgcaatct ccatacatca
    21661 aaaaaaatcc cgatcttctg accgggattt tcaacatcaa cgggaacttg attaaggcag
    21721 aattgacggc tgatccgccc cttctttttc cactttctgc tgcaacatgt gttcgcgctt
    21781 catacccagt ttcagagcca acgcagatgc tacgtagatg gaagatgcag tgccgatgga
    21841 aacaccgata agcatggtca gcgagaagcc ttccagtacc ggaccaccga agagatacag
    21901 catcaggata accatcaagg tagtaccgga tgtgatcaag gtacggtgca gcgtctgggt
    21961 caaggacacg ttaaagattt cgtaaggcgt accgcgacgg atcttgcgga agttttcacg
    22021 aattcggtcc gataccacga tactgtcgtt aagcgagtaa ccgataaccg acatcaacga
    22081 tgccacaatg gtcaggtcaa tctcgatatg gaataacgac aaaataccca gcgtaatgat
    22141 aacgtcgtgc gccagcgcaa taaccacccc tgccgccagt cgccactcaa agcggaaacc
    22201 tacgtacacg aggatagaca gcagcgctgc catcaacgcc atcgcaccgg tttgcgcaag
    22261 gtctgccccc acgctcggac cgacgaactc aatacgcttc actgctgcat tctgattggt
    22321 ggattcgtta atcaccttca gaacttggct gcccagcacc tgaccgccgg tttcgccttc
    22381 agcaggcggc atacggacca tgatgtcgtg gctgctacca aagttttgca gcattggctc
    22441 ttcaaaaccg gctttttgca atgcatcacg cattacgtca atttcagccg gtttttcgag
    22501 cgtaatttcg ataaccgtac caccggtgaa atccagcccc cagttaaagc cgcgcacgcc
    22561 cataataacg atagcagcga ttaacagcag accagagatg ccgaaagccc agtagtccca
    22621 gcgcataaag tcatagactt tacggccgtg gtttagttgt tcaacagtat attcctgtgc
    22681 cacatcgcac tcctcagatt gacagcttct tgacgcgctt gccgccatat agcaggttta
    22741 cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt cgccacaccg ataccggtag
    22801 taatcgcgaa ccctttaatt gccccggtac ccactgcgta caggatgatg actttaatca
    22861 gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc acgataacct tcatcaattg
    22921 cctgttgaac agtacgtccg ttgctcaact cttctttaat acgttcgttg atcagtacgt
    22981 tcgcatcgac cgctaccgca agggttaaga cgatacccgc gatacctggc atactcagtg
    23041 ttgcgcctgg caacagcgac ataataccga cgatcaagat caagttggca atcagagcac
    23101 tggtcgcaat cagaccaaac ttcttataga agatgatcat gaacagaata gaaaccagca
    23161 gaccggcaag gcaagcctcc agcccctgct caatgttctg catacccagg gtcgggccaa
    23221 ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc accggcacgc agcagcagtg
    23281 acagctgacg ggcttcgttc gggttgttga tgccggtgat acggaagctg ttacccagac
    23341 gagactggat gttggcgatg ttaatcacct cttcctgttt caccagaacc gcacgaccat
    23401 ttgcatcttt cttaccgctg tctttgtact ccacaaacag ggttgccatc ggtttgccga
    23461 tgttgtcctt ggtgaagtta gacataatgt tgccaccagc gctatcgagc gagatgttaa
    23521 cctgcggctg gttgtattcg tcctggctgg aagtggagtc ggtgatatgg tcaccagtca
    23581 gaattacgcg tttgtacagc acaaccggct gaccttcgcg ggtctgtttc acttcagagt
    23641 cgcccggtac gcgaccggat gccgcagcgg cctgatcaac attggtgttt accagacgga
    23701 attccagcgt tgccgtcgcg cccagaatct ctttcgcacg cgcagtgtcc tgaataccag
    23761 gcagttcaac aacgatacgg tcagcaccct gacgctgaac caccggctcc gccacgccaa
    23821 gttggtttac acggttacgc aggatattaa tgttctgctg caccgcatat tcacgcgctt
    23881 cactcagacg agcatcgctc attaccgcac gcagctggtt gctgccctgg ctgctaatca
    23941 ccaggtccgg atggcgcttg ctcagatacg caatggcttc atcacgagct ttagcatcgc
    24001 ggaaagtgat gctcaggccg tagttgtttt ctttacgaac agtggtatac gggatgccct
    24061 tttcgcgcag gtcactgcgt aggctatcga tattttgttc ctggagtttg ccaagcgcgg
    24121 tgtccatatc cacttccatc aggaagtgaa cgccgccacg caggtcaagg ccgagcttca
    24181 tcggctcagc atgaatagct gccagccagc gcggcgttgc cggggcaagg ttaagcgcca
    24241 cgacgtattt gtcacccata acgcccatta atgcttcacg agcgcgcaac tgggtgtcag
    24301 tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc cacagactta gcagttattt
    24361 tttcttcttg taacgttttc tggacctgga tcagcgtttg ctcactggcg gcgactccgc
    24421 gcgcaccagt gatctgaaca gccggatcct caccaaacag gttgggaagc gcatacagca
    24481 gaccgatgac aatcaccacg atcagcatga cgtacttcca caaaggataa cggtttaaca
    24541 cggcaattcc cttagggaaa aattttaatt acagcgcctt catggtgcct ttcggcagga
    24601 cggcagctac gaagtcacgt ttaataacta cttcagtggt gtcattcagc gcgatagcaa
    24661 tgtagccgtt ttccgctact ttggttacgc gaccaaccag gccaccgttc gtcagaactt
    24721 catcaccttt ggcaatggag tccatcagct ttttgtgttc tttggtgcgc ttctgctgtg
    24781 gacgcaggat catgaaatag aaaatcagac cgaataccac cagcatcaaa atcaaagaca
    24841 tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc atcagaaata aaaaagctca
    24901 ttaaatttcc ctcattatta atattaatca acgttcaaag gtggtacttc tcgcccctga
    24961 cgctggtaaa aatcagttac gaagctctct aatttaccct cttcaatagc cttgcgtaaa
    25021 cccgccatca aacgctggta gtaacgaagg ttatgaatgg tgttgagtcg cgcgcctaat
    25081 atttcgttgc aacggtcgag atgatgcaag taagcgcgtg aataattgcg acaggtgtag
    25141 caatcacact caggatcgag tgggccagta tcgctcttat atttcgcatt gcggattttc
    25201 accacgccat cggtcacgaa caaatgacca tttcgggcgt tgcgggttgg cattacgcag
    25261 tcaaacatat cgataccgcg acgtacgcct tcaaccaggt cttccggttt accaacgccc
    25321 atcaggtaac gcggtttgtc tgccggaatt tgcggacaca catgctccag aatacggtgc
    25381 atatctgctt tcggctcacc cacagccaga ccgccgacag cgtagccatc aaagccgata
    25441 tctaccagac ctttaacaga tatatcacgt aaatcttcgt aaatactgcc ctgaattata
    25501 ccaaataacg catttttgtt accaagactg tcaaaacgct cacggctacg cttcgcccaa
    25561 cgcagagaca tctccataga gcgttttgcg taatcccagt cagcaggata cggcgtacac
    25621 tcatcaaaga tcatgacgat atccgaacca agatcgtact gaatctccat cgacttttcc
    25681 gggtcgagga aaatcgggtc gccgttgatc gggttacgga agtgaacgcc ctgttcggtg
    25741 attttacgaa tatcaccaag gctgaagacc tggaagccgc cggagtcggt aagaatcggt
    25801 cctttccact gcataaaatc gtgcagatcg ccgtgcagtt tcatgatttc ctggcccggg
    25861 cgcagccaca ggtggaaagt gttgccgagg ataatttgcg cgccagtggc ttcaacttct
    25921 tccggcgtca tcccttttac ggtgccgtag gtgccaacag gcataaaaca aggcgtttcc
    25981 actacgccac gatcaaagac caggcggcca cggcgtgcgc gaccgtcggt ggtgtccagt
    26041 tcaaatttca ttttttctcc actacgtcag aaaaacagtc caacgtttaa accagcgccg
    26101 cggaattact ccccgacgcg ctcattaatt gcctgcggat tgtacgtgat aaacatcgca
    26161 tcaccgtaac taaaaaagcg atatttctct tctaccgccg ctttataggc attcatggtg
    26221 tgttgataac cggcaaaggc cgaaaccagc ataatcagcg tcgactctgg caagtggaag
    26281 ttcgtcacca gcgcatcgac cactttgtac tggaagcccg gatagataaa gatttgggta
    26341 tcgtcaaaga acggttcgat aagatcgttt tttgccgcct gtgccgcgct ttccagcgaa
    26401 cgtactgaag tggtgccaac cgcaatcacc cggttaccgc gcgctttcgc cgccagtacc
    26461 gcgtctacca catcctgcgg tacttcagcg tattccgagt gcatgatgtg atcttcaatg
    26521 gtgtcgacgc gcaccggctg gaaggtgccc gcaccaacgt gcaacgtcac aaacgccatc
    26581 tccacgcctt tggcgcgtaa tttttccagc aaaggctcgt caaaatgcag accagcggtc
    26641 ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt gataaagttc gcggtcagcg
    26701 tcttcgtccg gacgatcgat atacggcggc agcggcatat ggccgatgct gttgaggata
    26761 tccagtaccg agcgttcatc attaaattcg acttcaaaca gtgcgccgtg gcgcgcggtc
    26821 attgttgcgt tgatactttc gtcatcgccc agcaacagtt ctgcgccagg ttttggcgct
    26881 ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga gcatccgctc aaccagcact
    26941 tcaatcttgc cgccgctggc tttacgccca aacagacgcg ccgggatcac gcgggtatta
    27001 ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta aatcggtgaa agtaccgtgc
    27061 gtcagcgcac ccgtcggccc gtccagcgac agtaaacgac agctactgcg ttcaggcatg
    27121 ggatagtggg caatcaggga ttcgggcaat tcaaaggaga aatcggtaac gcgcatgaca
    27181 ctgactcaga ctaaaataag agtcgggtag tctagtgccg gggcgctctc cctgcaacct
    27241 ttacccccct ttggatagaa tataatcgat gaatttttta gctcacctgc atttagccca
    27301 tctcgcggaa agctcgcttt ctggcaattt actggctgat ttcgtacgcg gaaatcctga
    27361 agaaagtttt ccgcccgacg tcgtggctgg cattcatatg catcgacgta ttgactgaca
    27421 atctgccgga agtccgcgaa gcacgggagt ggtttcgtag tgaaacgcgc cgcgttgcgc
    27481 ctattacgct ggatgtcatg tgggatcact ttctttcccg ccactggtcg cagctgtcgc
    27541 cggactttcc gctacaggaa tttgtctgtt atgcccgcga gcaagtgatg acgattttgc
    27601 cggactcacc gccacgtttt atcaatctga acaattactt gtggtcagag cagtggctgg
    27661 tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca agccgccgcc
    27721 cacgtctgga tgccctgcgt gactcctggt acgatttaga cgctcattat gacgccctcg
    27781 aaccccgctt ctggcagttt tatccgcgga tgatggcgca ggcgtcacac aaggcgttat
    27841 aaaacgttag ttcatccata ccgtagccga aatggcaggc aaagccagaa tcccgtcagt
    27901 cagttgccca tgcccttctt tgcattgcca ttgcacggca ttgagaaacg gtgacgcggg
    27961 tagcaccact tcactggcct cgccacggtt gattgccacc agtacacgtt gctgattcag
    28021 cacgcggaca aataccacta cgttatcttc cgcatacagc acctgacagc cgccacgacg
    28081 cagcgcctga cttttcttac gcagcacaat cattcgccgg tacagcgcga ataacgccgt
    28141 atcctgcttt tccacctgcc aggggaacgg tttacggcaa gacggatcgt ttttaccatc
    28201 cagtcccact tcatcaccgt aataaatgca cggtacacca ggccaggtga acagccagac
    28261 caccgccagc ggcaggcgcg caatatcccg accgagcagc gttttaaatc gcgcagtatc
    28321 gtggctatcg agctgattaa acatacgtaa ttgttgttga tgagaaagcc ctgcgcggta
    28381 attatccatc caggccatac aggtttgggc gtcaatttgc tgcggatcat aagagatgtc
    28441 ggtattggca agaaatcccc acaacgggaa tgtgaagcca cgatagttca tggcagcatc
    28501 ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgttcgccga caatatacgc
    28561 ttccgactgg gtttctttcg ccgcttcggt gatcccggca acgtgctgca tattattgcg
    28621 cgccccaccc gcctccccca gcatatgcac cacatccagc cgccagccgt ccatattcca
    28681 cggcgctttc agccagtggc ggacaatact gtcttccccg cgataaattt cattcaccag
    28741 actttccgac tgataatcca gcttcggcaa gctggcatag ccaagccagt cgagcgccgt
    28801 gccatcatca ctaaacaagt accagtcgcg ccagggcgat tcagagttgt gacaagctcc
    28861 ccccgtgcca agattgtgcc tgtcaaacca ggcatgggaa tcgccactgt ggttaaacac
    28921 gccgtccagc accagccgca ttcccagctg ctgcgtattg tgtcgcaaac gcagcaacgc
    28981 cccatcaccg ccaaactgcg gatcgacatg gcgataatcc tcggtatcgt atttatgtac
    29041 gctgggagct ttaaacaccg gattgagata cagcgctgtc acgccaagct ttttcagata
    29101 cggcagtttt tcgcttatcc cgtccagatc gccgccatag aacgttgatc cgcccgcctg
    29161 cgccgtgacc ggttcatccc agtcacgcaa gatgatctct tgtccggctg catgatggta
    29221 atagacatga tcctgttcag cttcacgagg aagactacgc gcaaaacgat cagggaagat
    29281 ctgataaaaa atctgatccg cagcccattg tgggccgata tccggtacat cgacggcaaa
    29341 ctgctccagt cgtgccggcg gcattcggct gaagccctgc ggtgtaaacc aacgctggcg
    29401 atcgtgccac agcagtttga aactgtaacg ccgccggggt tgtccgctgg agagatcaat
    29461 cgccgcacgc catgcggtga cgcctggctg cggctgactg cgctgcttat gcattggtac
    29521 tgacatttct tcgttatcgt gttctgtacg cagcataatg cgctgcggtg ggtcttcgcc
    29581 cgtcagccac agtgtaatga gcagttgatc tttgctttgt ttaacaaatg ggggcaccgg
    29641 caggtgccat gcatttaaca tcataattcc cctgtgatga aaatctgatc accttgccat
    29701 agcctaatgc aggatcactc atcatctgaa ggatatttgg gggaggagga cgggggcgga
    29761 taactttcgg aatagccgga tcaggatttc ctcctgaacc ggcttacaga acgcaagtat
    29821 tattggtttt cagccagctg acgatcgtgg cggcgtttaa acatccagcc aatcaacagc
    29881 acaacaatcc acgcgaaacc gacatacagc gagatacgcg tatccgggtg ataaccaatc
    29941 aacccgataa taaagagcag gaaaatcagc ccgccgatgg tcgttgctac cccacccggc
    30001 actttaaatt tcagcgcctt aacttcttct ggcggcaaac ggcgacggaa ggcgatttgc
    30061 gacagcagga tcataatcca cacccatacc gtggcgaagg ttgccagcga agcgatcacc
    30121 aggaagacgt tttccggcat gatgtagttc agatacaccg caaacagcag cgcggtagtc
    30181 atcaccagca ccgtaaccca cggaataccg cgacgcgacg ttttgctgaa aattttcggt
    30241 gcgctgccct gctctgccat accgtggagc atacggccta cgccaaatac gtcactgtta
    30301 attgccgaca gcgaagcggt cagcacaaca aagttaagaa tgctggcggc aaaggtaatg
    30361 cccatatgct ggaacgtcag cacgaacggg ctaccggcag tgccaacctg attccacggg
    30421 tagatagaca taatgacgaa cagcgtaccg acgtagaaca ccagaatacg catcggcacg
    30481 gagttaatcg cacgcggaat cgatttctca ggatctttcg cttcaccggc ggtaatcccg
    30541 ataatttcga tcccaccgta agcaaacatc accatttgca acgacattac catgccaagc
    30601 cagccgttac tgaagaagcc gccgttgctc cacaggttat gaataccggt cggttgcccg
    30661 ccgttgccaa tcccccagat gatgatgccg aaaccggcga caatcatgat gatgatggtg
    30721 gcgactttaa agaacgagaa ccagaattcc agctcaccga agaccttcac gctcatcagg
    30781 tttacggcgc aaatgatcag caccacgctc agtacccaaa tccagtgcgg caccgtcggg
    30841 aacaagacac ccatatagat accaaaagcg gtcacatcgg cgatggcgac aataaggatt
    30901 tcaaagcagt aggtccatcc ggtaatgtaa cctgccagcg ggccaaggtt ttcctgcgca
    30961 taacgcgaga aagagctggc ggccgggtta tgtaccgaca tttcccccag cgcacgcata
    31021 atgatatacg ccgcgatacc accgataata taggccaaca acacgctcgg accggctatt
    31081 ttgatggcgt ctgccgaacc gtaaaacagc ccggtgccaa ttgctgaacc cagtgccata
    31141 aagcgtatgt ggcgggtact tagcccacgc tttagcttat tcttactttc catcaattcc
    31201 taacccatca acacaataaa aaaccacggg gcatcgcagc cccgtggtta aaacaaatgt
    31261 tcagtgattt agtgagcgct ggaggtcacc tgacgacctg ccgcgcgatc ccagataatg
    31321 gccagaacca ccatcaccac tgttggcatt aaccacgcca gaccttgttc ggccagcggt
    31381 aaacgctgcg cccaggacgg taagatatcg ctgaatgcag atgctttgat cccgtcgaga
    31441 ataccaaaaa gcaggctgat aaacatcggc ggagcaatca cgcgggacga attatgccac
    31501 catgagcgtg taaaacttaa tacaaccagt gcgatacacg gcggataaat agcggtcagc
    31561 accggtacgg agatctggat cagctggctt aagccgaggt tagacaccac catcgagaag
    31621 ccgccgagga taaacaccag cgtacgataa gagagcggta cgtactgggc aaagaattct
    31681 gcacaagcac aggtcaggcc aaccgccgtg accagacagg cgatgaagat taacgccgcc
    31741 agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt aagcatgcag aatagcagcg
    31801 ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac ccagacagaa cagcgccagg
    31861 tacagcaaag tcagaccaac acccgccatc aggccagccc agacggtata acgggtcagc
    31921 agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa caataacgat accaaacacc
    31981 attgcgccca gcgtatccat ggtcagatag ccgttaacga agccgttaga aaatgcagcg
    32041 ttttgataag cctcagtcgc cgtgctgata gaacccgccg gccagacaat cgcgacaaca
    32101 gacaggatga ccagcgcgat aattttcagc ggcgcaagga agttgcccac ggtatccagc
    32161 agcttgcccg gatagagcga aaccagaata acgatagcga aatagaccag gctgtagata
    32221 aacagcggca gcgcggaatc acccgtcagc ggcgcaatcc ccacttcaaa ggaaacggta
    32281 gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt aacaaaccgt tgccagcagt
    32341 acgccagcga ctttaccgat tggcgtgctg aggctgtcaa caccgccgcc aacttttgcc
    32401 agcgccacta ccgttaatac cggcaggcca acggcagtaa tgaggaagcc gaatgccgca
    32461 gtccagacgt gttcgcctgc ctgtaagccg accattggag ggaaaataat gttacctgcg
    32521 ccgacgaaca acgcaaatgt cataaagccc agagcgatga tatcgcgcga tcttaattga
    32581 tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa cgttaaattt ttgtgccaat
    32641 ccctggggat gatacagcgg tatatttaaa caattttagc gggaaatact cccaaccagg
    32701 cgcggcgaag aatagttttt cgatttaccg cgttaatgca gtcagtatga cagcacctga
    32761 agcgcaattt aaacgcttat aacgttttaa agcaaggagg gatctaaaaa ccagaggtat
    32821 atagcgataa gaaatcacca accagtataa aacactattt ccgtgaaaaa gacatggcta
    32881 atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa cgcccgtatt ttgactgcaa
    32941 agaaaataag ccagcatcgg ctggcttatg gcaataaaag atgacaaagg cggattaatc
    33001 gctgtttttg gcaattaaac gttccgggat aacaaaactg aaacgtgttc cttttcctac
    33061 tgtactctca atattcaggc gactttcgtg atgattcacc gcatgtttca cgatcgctaa
    33121 ccctaatccg ctaccgccgg tttgccggga acgcgcttta tcaacgcgat aaaaacgctc
    33181 ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg ttatcttcaa cgctaaattc
    33241 ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc gtgccttccg gcgtatgatt
    33301 cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc tgatcttcgt tgccagacac
    33361 cttgaggccg ttatctatct caaaggtaaa tgtctgtttt ttctgactca gagtctgagc
    33421 ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc ttttcattga gcaattgcgt
    33481 cggcgaggct tcaattttcg acagcgttag caactgcttc accagccctt ccatccgctg
    33541 ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc gcgccttcca gcggctgctc
    33601 atccatcatc tccaggtaac cctgtaacac ggtcaatggc gtacgtaact catggctcac
    33661 gttggcaaaa aagttacgcc gcgccccttc cagttgatgc atttgcgtga catcacgcgc
    33721 caccatcaac aactgtttgt gggtataagg catgacgcga atttccagat gccgcccggt
    33781 gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt ttcagatatt gcgtaaactc
    33841 cgggtaacgc agtaggttaa ggatgttctg cccgttatct tccggccagc gcaaaccaag
    33901 aatttgttgc gccagaccgt tacaccagaa aataccgccc tcttccgtgg tcagcaccac
    33961 cgcgtcgggc agcgactccg cgccgctacg aaagcgttta atcagattgc ccagttcacg
    34021 gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg tatagtagcg gttcccagct
    34081 accacgcccc ggtggcgggg tcatactgcg atccacccac agccaccatg aaaggcgcaa
    34141 taaattccag aaatgccaga taagcagtcc tgttaccgat gccagcaaaa accagggcag
    34201 gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag caaagtagca gctccagcac
    34261 cagccttttc cacgacagcc gttccagcac gtaagatact ccagttaaga aatcataagc
    34321 cctgctctgc gtccgatgag caaggcgtta aaagcgggtt gaaaaacgat atcctgtacc
    34381 gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc agtgctttac gcaggcgacg
    34441 aatgtggaca tcgaccgtgc ggtcttccac atacacgtta gttccccaga cgtggtttaa
    34501 cagctgctcg cggctgtaca cgcgctcagg atgcgtcata aaaaagtgca gcagtttaaa
    34561 ttctgtcggc cccatctcca gcggctcttc gcccgccatc actcggtgag atgtcgggtc
    34621 gagacttaat ccctgcatct caatcacctc ttccaccgcc attggcgaaa tacggcgcat
    34681 taccgctttg attcgcgcca ccagctcctt cggcgaaaat ggcttggtga tatagtcatc
    34741 cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc cctctggcgg tcaacatcac
    34801 cactggaata tcccgggtca tcgactcgcg cttgaggtgt ttgatgaact ggataccgga
    34861 gccgccaagt aacatccagt caaggagaat taaatccggc caaggttcat tcagttgatt
    34921 cacagcactg tcataatctt ccgcttcgac cggctgaaag ccattttgtt cgagcacgaa
    34981 gcagaccatt tcgcgaattg gagcttcatc ttctacgacc agaatacgtc tcgccatgat
    35041 ttgccctgtt gtaataaata ggttgcgatc attaatgcga cgtcattatg cgtcagattt
    35101 atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg ttattgattt ccgtggcgca
    35161 gaaaaaagca aatggcacat ctgtttgggt ataatcgcga ccatgctttt tcgccaggga
    35221 accgttatgc gcatccttca cacctcagac tggcatctcg gccagaactt ctacagtaaa
    35281 agccgcgaag ctgaacatca ggcttttctt gactggctgc tggagacagc acaaacccat
    35341 caggtggatg cgattattgt tgctggtgat gttttcgata ccggctcgcc gcccagttac
    35401 gcccgcacgt tatacaaccg ttttgttgtc aatttacagc aaactggctg ccatctggtg
    35461 gtactggcag gaaaccatga ctcggtcgcc acgctgaatg aatcgcgcga tatcatggcg
    35521 ttcctcaata caaccgtggt cgccagcgcc ggacatgcgc cgcaaatctt gcctcgtcgc
    35581 gacgggacgc caggcgcagt gctgtgcccc attccgtttt tacgcccgcg tgacattatt
    35641 tccagccagg cggggcttaa cggtattgaa aaacagcagc atttactggc agcgattacc
    35701 gattattacc aacaacacta tgccgatgcc tgcaaactgc gcggcgatca gcctctgccc
    35761 atcatcgcca cgggacattt aacgaccgtg ggtgccagta aaagtgacgc cgtgcgtgac
    35821 atttatattg gcacgctgga cgcgtttccg gcacaaaact ttccaccagc cgactacatc
    35881 gcgctcgggc atattcaccg cgcacagatt attggcggca tggaacatgt tcgctattgc
    35941 ggttccccca ttccactgag ttttgatgaa tgcggtaaga gtaaatatgt ccatctggtg
    36001 acattttcaa acggcaaatt agagagcgtg gaaaacctga acgtaccggt aactcaaccc
    36061 atggcggtgc tgaaaggcga tctggcgtcg attaccgcac agctggaaca gtggcgcgat
    36121 gtatcgcagg agccacctgt ctggctggat atcgaaatca ctactgatga gtatctgcat
    36181 gatattcagc gcaaaatcca ggcattaacc gaatcattgc ctgtcgaagt attgctggta
    36241 cgtcggagtc gtgaacagcg cgagcgtgtg ttagccagcc aacagcgtga aaccctcagc
    36301 gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac tggaagaact ggatgaatcg
    36361 cagcagcaac gtctgcagca tcttttcacc acgacgttgc ataccctcgc cggagaacac
    36421 gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa ctcattaaaa ggcgaatgga
    36481 agattgattt cacccgcgag ccgttcgcca gcaacgggct gtttgccatt accggcccaa
    36541 ccggtgcggg gaaaaccacc ctgctggacg ccatttgtct ggcgctgtat cacgaaactc
    36601 cgcgtctctc taacgtttca caatcgcaaa atgatctcat gacccgcgat accgctgaat
    36661 gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta tcgcgcgttc tggagccaga
    36721 atcgggcgcg taaccagccg gacggtaatt tgcaggtgcc tcgcgtagag ctggcgcgct
    36781 gcgccgacgg caaaatcctc gccgacaaag tgaaagataa gctggaactg acagcgacgt
    36841 taaccgggct ggattacggg cgcttcaccc gttcgatgct gctttcgcag gggcaatttg
    36901 ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt gctcgaggag ttaaccggca
    36961 ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca gcacaaatcg gcccgcactg
    37021 agctggagaa gctgcaagcg caggccagcg gcgtcacgtt gctcacgccg gaacaagtgc
    37081 aatcgctgac agcgagtttg caggtactta ctgacgaaga aaaacagtta attaccgcgc
    37141 agcagcaaga acaacaatcg ctaaactggt taacgcgtca ggacgaattg cagcaagaag
    37201 ccagccgccg tcagcaggcc ttgcaacagg cgttagccga agaagaaaaa gcgcaacctc
    37261 aactggcggc gcttagtctg gcacaaccgg cacgaaatct tcgtccacac tgggaacgca
    37321 tcgcagaaca cagcgcggcg ctggcgcata ttcgccagca gattgaagaa gtaaatactc
    37381 gcttacagag cacaatggcg cttcgcgcga gcattcgcca ccacgcggcg aagcagtcag
    37441 cagaattaca gcagcagcaa caaagcctga atacctggtt acaggaacac gaccgcttcc
    37501 gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt ctcccaacaa accagcgatc
    37561 gcgagcatct gcggcaatgg cagcaacagt taacccatgc tgagcaaaaa cttaatgcgc
    37621 ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc taccgccctg gcgcaacatg
    37681 ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca tgggcagatt gttccccaac
    37741 aaaaacgtct ggcgcagtta atggtcacta tccagaatgt cactctggaa cagacgcaac
    37801 gtaatgtcgc acttaacgaa atgcgccagc gttataaaga aaagacgcag caacttgccg
    37861 atatgaaaac catttgcgag caggaagcgc gcatcaaaac gctggaagcc cagcgtgcac
    37921 agttacaggc gggtcagcct tgtccacttt gtggttccac cagccacccg gcggtcgagg
    37981 cgtatcaggc gctggagcct ggtgttaatc agtctcgatt actggcgctg gaaaacgaag
    38041 ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca actggatgca ttaacgaagc
    38101 agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca agatgagcaa gcacttactc
    38161 aacaatggca agccgtcacg gccagcctca atatcacctt gcagccacag gacgatattc
    38221 aaccgtggct ggatgcacaa gatgagcacg aacgccagct gcggttactc agccaacgac
    38281 atgaattaca agggcagatt gccgcgcata atcagcaaat tatccagtat caacagcaaa
    38341 ttgaacaacg tcagcagcag ctcttaacgg cattggcggg ttatgcactg acattgccac
    38401 aggaagatga agaagagagc tggttggcga cacgtcagca agaagcgcag agctggcagc
    38461 aacgccagaa cgaattaacc gcgctgcaaa accgtattca gcagctgacg ccgattctgg
    38521 aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga aactgtggag ctggataact
    38581 ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca gcagcagacg ttacagcaac
    38641 aggatgttct ggcggcgcaa agtctgcaaa aagcccaagc gcagtttgac accacgctac
    38701 aggccagcgt ctttgacgat cagcaggcgt tccttgcggc gctaatggat gaacaaacac
    38761 taacgcagct ggaacagctc aagcagaatc tggaaaacca gcgccgtcag gcgcaaacgc
    38821 tggtcactca gacagcagaa acgctggcac agcatcaaca acaccgacct gacgggttgg
    38881 ctctcactgt gacggtggag cagattcagc aagagttagc gcaaactcac caaaagttgc
    38941 gtgaaaacac cacgagtcaa ggcgagattc gccagcagct gaagcaggat gcagataacc
    39001 gtcagcaaca acaaacctta atgcagcaaa ttgctcaaat gacgcagcag gttgaggact
    39061 ggggatatct gaattcgcta ataggttcca aagagggcga taaattccgc aagtttgccc
    39121 aggggctgac gctggataat ttagtccatc tcgctaatca gcaacttacc cggctgcacg
    39181 ggcgctatct gttacagcgc aaagccagcg aggcgctgga agtcgaggtt gttgatacct
    39241 ggcaggcaga tgcggtacgc gatacccgta ccctttccgg cggcgaaagt ttcctcgtta
    39301 gtctggcgct ggcgctggcg ctttcggatc tggtcagcca taaaacacgt attgactcgc
    39361 tgttccttga tgaaggtttt ggcacgctgg atagcgaaac gctggatacc gcccttgatg
    39421 cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt gattagccac gtagaagcga
    39481 tgaaagagcg tattccggtg cagatcaaag tgaaaaagat caacggcctg ggctacagca
    39541 aactggaaag tacgtttgca gtgaaataac tattcagcag gataatgaat acagaggggc
    39601 gaattatctc ttggccttgc tggtcgttat cctgcaagct atcactttat tggctacggt
    39661 gattggtagc cgttctggtg gttgtgatgg tggtatgaaa aaagtcattt tatctttggc
    39721 tctgggcacg tttggtttgg ggatagccga atttggcatt atgagcgtgc tcacggagct
    39781 ggcgcataac gtaggaattt cgattcctgc tgccgggcat atgatctcgt attatgcact
    39841 ggtggtggtg gtcggtgcgc caatcatcgc actcttttcc agccgctact cactcaaaca
    39901 tatcttgttg tttctggtgg cgttgtgcgt cattggcaac gccatgttca cgctctcttc
    39961 gtcttacctg atgctcgcca ttggtcggct ggtatccggc tttccgcatg gcgcattttt
    40021 tggcgtcgga gcgatcgtgt tatcaaaaat tatcaaaccc ggaaaagtca ccgccgccgt
    40081 ggcggggatg gtttccggga tgacagtcgc caatttgctg ggcattccgc tgggaacgta
    40141 tttaagtcag gaatgctggc gttacacctt tttattgatc gctgttttta atattgcggt
    40201 gatggcatcg gtctattttt gggtgccaga tattcgcgac gaggcgaaag gaaatctgcg
    40261 cgaacaattt cactttttgc gcagcccggc cccgtggtta attttcgccg ccacgatgtt
    40321 tggcaacgca ggtgtgtttg cctggttcag ctacgtaaag ccatacatga tgtttatttc
    40381 cggtttttcg gaaacggcga tgacctttat tatgatgtta gttgggctag ggatggtgct
    40441 gggaaatatg ctaagtggca ggatttcagg acgttattca ccactgcgca ttgcagcagt
    40501 gactgacttt atcattgtac tggcactgct gatgctcttt ttctgcggcg gcatgaaaac
    40561 aacgtcgctt atttttgctt ttatttgttg cgcgggatta tttgcccttt cagcacctct
    40621 gcaaatattg ttactgcaaa atgccaaagg cggagagtta ttaggtgccg caggtgggca
    40681 aatagcgttt aacctcggta gcgccgtcgg cgcatattgc ggaggtatga tgctgacgct
    40741 ggggctggca tataattacg tggcgctgcc tgccgccctg ttttcgtttg ctgcgatgtc
    40801 atcgttgctg ctgtatggtc gctataagcg ccagcaagcg gcggatagtc cggtgctggc
    40861 gaaaccactg gggtagatta ctgtcacggt gcgcgttgct tattgccgga tgcggcgtga
    40921 acgccttatc cgccctacgc ggttctggca cattttgtag gcctgataag acgcggcaag
    40981 cgtcgcatca ggcatgatgc gccaattgcc tacgtttttt actcttgtgg ccataaccac
    41041 gcagcgccgc gtacgccgct ggaatcaccg tgcttcgcct tactcaccgg cgtttcacat
    41101 tcgccgccga agacaaattg tttaatcaac tgcccaaccg tttgatataa acggtctaca
    41161 ttgctcatcc cgccccccag gacaatcaca tccggatcga gaatattcac gacatgtgcc
    41221 agcgattttg ccagccgcag ctcgtagcga cgcaatgcca gttccgctac cggatcgctt
    41281 tcttcaacca ggcggataat ttcactgcct ttcagcgcat gtccgctcaa acgacgataa
    41341 tccgtcgcga atcccgtgcc cgaaataaag gtttcaatac aaccttgttt accgcaataa
    41401 caagggactt cctcgcgata acgcagttcg tcttcgtcca tccacggtag cggattgtgt
    41461 ccccactcac ctgccgtgcc attgccgccg atatgcgtcc gcccattgaa tgccacgccc
    41521 gcgccgcatc ccgtgccgat aatcacggca aataccgtct gcgctcccgc tgccgcgcca
    41581 tctactgctt ctgaaaccgc cagacagttg gcatcatttg ccagccgcac ttcccgctgc
    41641 aacctcgcgc ttaagtcttt atcgaatggc tgaccgttga gccaggttga attggcattc
    41701 ttcaccacac cggtgtaggg cgaaatcgaa cctggaatgc ccatacctac cgttccgcgc
    41761 tgccctgtcg cctgctccgc catatcaacc aacgtggcga tcgtttcaat agtctgccgg
    41821 taatcatcac gcggcgtggg cagacgatgg cggtacaact gctcccctgc atcgcccagt
    41881 gcaatcactt cagttttggt gccgcctaaa tcgataccta tacgcactgt actctcctta
    41941 tttttttcaa tatcaatagc gtagagacgg acaaccggat tggcaatgca aggccgccta
    42001 caattcgtta tcatgcccgc taaatttaac gacaaggccg tggaaattat catgctgtgg
    42061 ttcaaaaatt taatggttta ccgtcttagc cgcgagattt cgctgcgtgc agaagagatg
    42121 gaaaaacagc tagcctcgat ggcatttacc ccatgcggca gccaggacat ggcgaagatg
    42181 ggctgggttc ctccgatggg atcgcacagc gatgcgttaa cgcacgttgc caatggtcaa
    42241 attgttatct gcgcgcgcaa agaagaaaaa atcctcccgt ctccggtgat taaacaggcg
    42301 ctggaagcga aaatcgccaa actggaagcg gaacaggcgc gtaagctgaa gaaaaccgaa
    42361 aaagattcgc tgaaagacga agtgctgcac tctctgctgc cgcgtgcttt cagccgtttt
    42421 agccagacaa tgatgtggct cgacacggtt aacggtttga ttatggtgga ctgcgccagt
    42481 gccaaaaaag cggaagatac gctggcatta ctgcgtaaaa gcctggggtc gttaccggtt
    42541 gtaccattga gcatggaaaa cccgattgaa ctgacgctga ccgaatgggt tcgctccggt
    42601 agtgcggcac agggcttcca gctgcttgat gaagccgagc tgaaatcgtt gctggaagat
    42661 ggcggcgtga tccgcgcgaa gaaacaagat ctgaccagcg aagagatcac caatcacatt
    42721 gaagccggaa aagtggtgac taaactggcg ctcgactggc agcagcgcat tcagtttgtg
    42781 atgtgcgacg atggttcgct caagcgtctt aagttctgcg acgagctgca cgatcaaaac
    42841 gaagatatcg accgtgaaga tttcgcccag cgttttgatg ccgatttcat cctgatgact
    42901 ggtgaactgg cagcgttaat tcaaaacctg attgaaggat taggtggcga agcacaacgt
    42961 taattgctga ttttccttta atgccggatg cgacgcctgc cgcgtcttat ccggcgtacg
    43021 aagccacacc aggcatataa ttattcgcta cggcgagcaa taatttttag cgcagcaata
    43081 ttatgcgttt tacgccgtaa ctgatctaat cgcctcatcc ctattcgcct gccagcagca
    43141 cgcagaatat tcactaaccc ccatttatcg ctggtaaatt gttttgttaa cagctccgct
    43201 tttggggtat caatatattc acgcaataaa tcagaaattg aacaaatatc cgtcaggtaa
    43261 ggataccagc aagtttcacc gtgaaaatta cgcactccag cgttacctgc aataaagtct
    43321 ttcgggtaat cccagatcac gtttttatcc aacgtgatcc aataacgcgg taaatcggta
    43381 cttccaccat tttggctgcg catgggataa cgggtacagt gaatttgcag gttgatcgtt
    43441 ggcgtcagta agtcatagat ctcccgctgt aacttgctcc atggacgttg tgtcattgtt
    43501 tttcctcaag ccggtgacgc gcttcactgc gaattttccg cgttgccccg gttttcacta
    43561 aatcacgtaa ctgcgaaaga tcgggttgtt cgacaaataa gagtaatgat ggatcctctt
    43621 ttaaccacgc cgttttcaca tcggcagcca gctgtggatt attaatagcc agcgaccgat
    43681 cctgaggctc agtcaatgtc gttaacaacg acgccggggt atgtgcgttg cgtaatgccg
    43741 tttggcgcaa atgatcattg cgactttgca gcaatgcacg ccaccattca tcatctgatt
    43801 tatcttttat cgtagccagt tccgcaaaaa atacctggag
//
LOCUS       DADLXX010000019        43521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLXX010000019 DADLXX010000000
VERSION     DADLXX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.019"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            231..1664
                     /locus_tag="JNX27_001031"
     CDS             231..1664
                     /locus_tag="JNX27_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY7882320.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JNX27_001032"
     CDS             complement(1672..2730)
                     /locus_tag="JNX27_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7882321.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JNX27_001033"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JNX27_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JNX27_001034"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JNX27_001034"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY7882322.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNX27_001035"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNX27_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7882323.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNX27_001036"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNX27_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7882324.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JNX27_001037"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JNX27_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7882325.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JNX27_001038"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JNX27_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7882326.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLANPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRHRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="JNX27_001039"
                     /pseudo
     CDS             complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="JNX27_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="JNX27_001040"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="JNX27_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7882327.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="JNX27_001041"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="JNX27_001041"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7882328.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="JNX27_001042"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="JNX27_001042"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7882329.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="JNX27_001043"
     CDS             complement(17698..18321)
                     /locus_tag="JNX27_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7882330.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /locus_tag="JNX27_001044"
     CDS             18618..18893
                     /locus_tag="JNX27_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882331.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="JNX27_001045"
     CDS             complement(18970..19212)
                     /locus_tag="JNX27_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7882332.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="JNX27_001046"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="JNX27_001046"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7882333.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="JNX27_001047"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="JNX27_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7882334.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="JNX27_001048"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="JNX27_001048"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7882335.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22591..24288
                     /locus_tag="JNX27_001049"
     CDS             22591..24288
                     /locus_tag="JNX27_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7882336.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24303..25037
                     /locus_tag="JNX27_001050"
     CDS             24303..25037
                     /locus_tag="JNX27_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7882337.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="JNX27_001051"
     CDS             25050..25907
                     /locus_tag="JNX27_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7882338.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNX27_001052"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNX27_001052"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7882339.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNX27_001053"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNX27_001053"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7882340.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNX27_001054"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNX27_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="JNX27_001055"
     CDS             complement(30571..31818)
                     /locus_tag="JNX27_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7882341.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGENGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="JNX27_001056"
     CDS             complement(31840..32166)
                     /locus_tag="JNX27_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7882342.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNX27_001057"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNX27_001057"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7882343.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="JNX27_001058"
     CDS             33554..34810
                     /locus_tag="JNX27_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7882344.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="JNX27_001059"
     CDS             34925..35251
                     /locus_tag="JNX27_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7882345.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35298..36536)
                     /gene="mntH"
                     /locus_tag="JNX27_001060"
     CDS             complement(35298..36536)
                     /gene="mntH"
                     /locus_tag="JNX27_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7882346.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36872..38074
                     /gene="nupC"
                     /locus_tag="JNX27_001061"
     CDS             36872..38074
                     /gene="nupC"
                     /locus_tag="JNX27_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7882347.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38124..40313)
                     /locus_tag="JNX27_001062"
                     /pseudo
     CDS             complement(38124..40313)
                     /locus_tag="JNX27_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40521..40596)
                     /locus_tag="JNX27_001063"
     tRNA            complement(40521..40596)
                     /locus_tag="JNX27_001063"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40561..40563),aa:Ala,seq:ggc)
     gene            complement(40636..40711)
                     /locus_tag="JNX27_001064"
     tRNA            complement(40636..40711)
                     /locus_tag="JNX27_001064"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40676..40678),aa:Ala,seq:ggc)
     gene            40947..41291
                     /locus_tag="JNX27_001065"
     CDS             40947..41291
                     /locus_tag="JNX27_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7882348.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            41314..41685
                     /locus_tag="JNX27_001066"
     CDS             41314..41685
                     /locus_tag="JNX27_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7882349.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="JNX27_001067"
     CDS             complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="JNX27_001067"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7882350.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43411..43486
                     /locus_tag="JNX27_001068"
     tRNA            43411..43486
                     /locus_tag="JNX27_001068"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43444..43446,aa:Val,seq:tac)
BASE COUNT        11558 a        10837 c        10116 g        11010 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaagctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggcaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcatcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgattctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc taggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgctgca gggtcgataa ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggtagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccctca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatattgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata atgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagatac cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc cgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gctacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac gggattattg aggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgcc
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgtgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgaga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcttatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggtgtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    23041 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    23101 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    23161 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    23221 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    23281 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    23341 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    23401 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    23461 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    23521 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    23581 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    23641 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    23701 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    23761 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    23821 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag
    23881 caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc
    23941 tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag
    24001 ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc
    24061 attgcggtgc gagataccgg gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg
    24121 aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg
    24181 tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt
    24241 cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata
    24301 ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa
    24361 ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga
    24421 agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg
    24481 atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca
    24541 tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc
    24601 tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc
    24661 aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taaaatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg caccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccatt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgta tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaagattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acctgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca gatattcagc
    35341 gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct gttcaccaga
    35401 tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc cagggcgata
    35461 ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga
    35521 atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg gatatggaag
    35581 cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt
    35641 ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa cagcggttgc
    35701 agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc
    35761 gccgcagctg tagccatcat cgccagattg acaaaaccgg caatcgtcat ggcgatagcc
    35821 acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg
    35881 agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcctgctgcc
    35941 aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc cagctgcgcc
    36001 aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac
    36061 ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa
    36121 gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa
    36181 ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga
    36241 acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc cgccagattt
    36301 ttaccggtgg caatccctag tttggcagag aggatctgaa ttagcatcgc catcaggttg
    36361 gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg aatattggtc
    36421 gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg
    36481 agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg
    36541 tgcctctaaa acatagcctt tgctatgttt catgctatgc taaacgagaa tgattatcaa
    36601 attcatttaa atggattgtg gtgatttctc tgatagacca agattatgat tacgaaaaga
    36661 ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa ctttcagctt
    36721 ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagcct gtcacaggac
    36781 gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg
    36841 aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc
    36901 acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat
    36961 ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc
    37021 cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tactcggatt
    37081 tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt
    37141 cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta ttctccagca
    37201 cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca aagtcaacgg
    37261 tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc agtcagaaaa
    37321 ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt acaccatggc
    37381 tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga ccatgctgga
    37441 accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta tcgtgctgtc
    37501 gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt ccaacctgca
    37561 cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc
    37621 cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc tgaacgcact
    37681 gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca tcctgggcta
    37741 catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg
    37801 cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa
    37861 aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt
    37921 cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga atgaagagca
    37981 aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt
    38041 gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa gccggggatc
    38101 attccccggc ttttttatgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
    38161 ccttgctgat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
    38221 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
    38281 atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
    38341 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggcgccaaa gtcatcaatc
    38401 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
    38461 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
    38521 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
    38581 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
    38641 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
    38701 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
    38761 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
    38821 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
    38881 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
    38941 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
    39001 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
    39061 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
    39121 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
    39181 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
    39241 tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    39301 cgaaagttgg gtaggagcgt taacggatct gttagcgcct gcaaatgcca gcggcgatta
    39361 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    39421 agcaccgcga gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    39481 ttgtaattca gaaggcaaag cgttgaaaca gcctaggtga gatttaaaaa cggatagcga
    39541 agattaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    39601 aagtcgtttt cataaggtgt gcacaacaga agtaggagca cgcttaacgc tgccagccag
    39661 cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct gcgccacaat
    39721 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    39781 atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg
    39841 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    39901 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    39961 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    40021 aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag
    40081 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    40141 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    40201 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    40261 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    40321 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    40381 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
    40441 aacccggacg taaactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
    40501 tgcagcgagg gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
    40561 gccatgcaag cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt
    40621 gctgggtgca aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    40681 gcaagcgctc tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc
    40741 ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc
    40801 gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat
    40861 gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc
    40921 agggaattgt tatgtccaag gagaggatga caccagatga acttgccaga ctgaccggct
    40981 atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac
    41041 ctggcgtcca gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata
    41101 ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc
    41161 cgcttgaagt attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt
    41221 ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg
    41281 atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc
    41341 ctcggtgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa
    41401 aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc
    41461 gagtttattc agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag
    41521 agaattgctg aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg
    41581 atagataata tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga
    41641 attcgcaatt tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg
    41701 atattatctc ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc
    41761 aataaaatcc agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac
    41821 ggtaacatcc agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc
    41881 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    41941 ttcagcggtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
    42001 cgctaccgga cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta
    42061 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    42121 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    42181 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    42241 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
    42301 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    42361 cagatagttc agtagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    42421 tgccccgtga cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc
    42481 gtaaaccggc accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt
    42541 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
    42601 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
    42661 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    42721 gttagcaaaa cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca
    42781 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    42841 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
    42901 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
    42961 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    43021 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
    43081 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    43141 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    43201 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc
    43261 taccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    43321 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    43381 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
    43441 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag
    43501 tctccgccgt gtagcaagaa a
//
LOCUS       DADLXX010000020        43461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLXX010000020 DADLXX010000000
VERSION     DADLXX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.020"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..413
                     /locus_tag="JNX27_001069"
     CDS             <1..413
                     /locus_tag="JNX27_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7882351.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="JNX27_001070"
                     /pseudo
     CDS             438..3037
                     /locus_tag="JNX27_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="JNX27_001071"
                     /pseudo
     CDS             3028..4098
                     /locus_tag="JNX27_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4110..4652
                     /locus_tag="JNX27_001072"
     CDS             4110..4652
                     /locus_tag="JNX27_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7882352.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="JNX27_001073"
     CDS             4660..5175
                     /locus_tag="JNX27_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7882353.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="JNX27_001074"
     CDS             5141..5878
                     /locus_tag="JNX27_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7882354.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="JNX27_001075"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="JNX27_001075"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7882355.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="JNX27_001076"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="JNX27_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7882356.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNX27_001077"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNX27_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7882357.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNX27_001078"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNX27_001078"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7882358.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="JNX27_001079"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="JNX27_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="JNX27_001080"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="JNX27_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7882359.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="JNX27_001081"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="JNX27_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7882360.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="JNX27_001082"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="JNX27_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7882361.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="JNX27_001083"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="JNX27_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7882362.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNX27_001084"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNX27_001084"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7882363.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="JNX27_001085"
     CDS             complement(17760..19520)
                     /locus_tag="JNX27_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7882364.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="JNX27_001086"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="JNX27_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7882365.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNX27_001087"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNX27_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7882366.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNX27_001088"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNX27_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7882367.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="JNX27_001089"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="JNX27_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7882368.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNX27_001090"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNX27_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7882369.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="JNX27_001091"
     CDS             complement(25127..25573)
                     /locus_tag="JNX27_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7882370.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="JNX27_001092"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="JNX27_001092"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7882371.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNX27_001093"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNX27_001093"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7882372.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="JNX27_001094"
     CDS             complement(28336..28998)
                     /locus_tag="JNX27_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7882373.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="JNX27_001095"
     CDS             29171..29584
                     /locus_tag="JNX27_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7882374.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNX27_001096"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNX27_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7882375.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNX27_001097"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNX27_001097"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7882376.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="JNX27_001098"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="JNX27_001098"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7882377.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNX27_001099"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNX27_001099"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7882378.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNX27_001100"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNX27_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7882379.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="JNX27_001101"
     tRNA            complement(32850..32937)
                     /locus_tag="JNX27_001101"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="JNX27_001102"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="JNX27_001102"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7882380.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36253
                     /gene="hyaB"
                     /locus_tag="JNX27_001103"
                     /pseudo
     CDS             34478..36253
                     /gene="hyaB"
                     /locus_tag="JNX27_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36272..36979
                     /gene="hyaC"
                     /locus_tag="JNX27_001104"
     CDS             36272..36979
                     /gene="hyaC"
                     /locus_tag="JNX27_001104"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7882381.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36976..37563
                     /locus_tag="JNX27_001105"
     CDS             36976..37563
                     /locus_tag="JNX27_001105"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7882382.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37560..37958
                     /gene="hyaE"
                     /locus_tag="JNX27_001106"
     CDS             37560..37958
                     /gene="hyaE"
                     /locus_tag="JNX27_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7882383.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37955..38812
                     /locus_tag="JNX27_001107"
     CDS             37955..38812
                     /locus_tag="JNX27_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7882384.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38946..40490
                     /gene="appC"
                     /locus_tag="JNX27_001108"
     CDS             38946..40490
                     /gene="appC"
                     /locus_tag="JNX27_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7882385.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40502..41638
                     /gene="appB"
                     /locus_tag="JNX27_001109"
     CDS             40502..41638
                     /gene="appB"
                     /locus_tag="JNX27_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7882386.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41650..41742
                     /gene="cbdX"
                     /locus_tag="JNX27_001110"
     CDS             41650..41742
                     /gene="cbdX"
                     /locus_tag="JNX27_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7882387.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41822..43120
                     /locus_tag="JNX27_001111"
     CDS             41822..43120
                     /locus_tag="JNX27_001111"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7882388.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43235..>43461)
                     /locus_tag="JNX27_001112"
     CDS             complement(43235..>43461)
                     /locus_tag="JNX27_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882389.1"
                     /translation="AVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIK
                     GAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10809 a        10587 c        11566 g        10499 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttgctg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacga ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gctagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcggacg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccggact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctagcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagctgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggatgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagta gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 catttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgcaaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacatat
    34981 ttcttcgacg tacaaaatcg cctgaagaaa ttcgttgaag gcgggcagtt ggggatcttc
    35041 cgcaatggct actgggggca cccgcagtac aaattgccgc cagaagccaa cctgatgggc
    35101 tttgcccact atctcgaagc tctcgatttc caacgtgaaa ttgtcaaaat tcacgcggtc
    35161 tttggcggta aaaacccgca tccaaactgg attgtcggcg ggatgccttg cgccattaac
    35221 attgacgaaa gcggcgcggt cggggcggtc aatatggaac gcctgaacct ggtgcagtcg
    35281 attatcaccc gcacggcaga cttcattaac aacgtgatga tccccgacgc cttagccatc
    35341 ggtcagttca acaaaccgtg gagcgaaatc ggcacgggtc tttctgataa atgtgtcctc
    35401 agctacggcg cattcccgga tattgccaac gactttggtg agaaaagtct gttgatgcct
    35461 ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc cagtggattt ggttgatccg
    35521 cagcaggtgc aggagtttgt cgatcacgcc tggtatcgtt atcccaacga tcaggtcggg
    35581 cgtcatccat tcgatggcat taccgacccg tggtacaacc ccggcgatgt caaaggcagc
    35641 gataccaaca ttcagcagct gaatgaacag gaacgctact cgtggatcaa agcgccacgc
    35701 tggcgcggta acgcgatgga agtggggccg ctggcacgca cgttaatcgc ttatcacaaa
    35761 ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt cggcgttgaa cctgccgctt
    35821 tccggtatcc agtcaacgtt gggccgcagg taagttgcag tatttcttcg acaagctgat
    35881 gaccaacctg aaaaatggca atctcgccac tgcttccact gaaaaatggg aacctgcaac
    35941 ctggccgaca gagtgccgtg gcgtcggctt taccgaagcg ccgcgcggag cgttaggcca
    36001 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
    36061 gaacgccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa
    36121 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
    36181 tccgtgcctc gcctgttcaa ctcatgtgct gggcgacgac ggtagcgagc tgatctccgt
    36241 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
    36301 ccactatgtc tttgaagcac cagttcgtat ctggcactgg ctgacggtgt tatgcatggc
    36361 ggtgttgatg gtcaccggtt actttatcgg caagccgcta ccttccgtca gtggcgaggc
    36421 gacgtatctg ttctatatgg gttacatcag gttaattcac ttcagcgccg ggatgatttt
    36481 taccgtggtc ttgctgatgc ggatctactg ggcttttgtt ggcaatcgtt actcccgcga
    36541 gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
    36601 ctggtatctg tttctggcaa aacgtccgag tgctgatata ggccataacc ccatcgccca
    36661 ggcggcgatg ttcggctatt tcctgatgtc ggtctttatg atcatcaccg gttttgcgct
    36721 gtacagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
    36781 ctactggacg ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
    36841 gattggggcg tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga
    36901 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
    36961 taacaaggag cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
    37021 tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg
    37081 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
    37141 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaagg
    37201 ctgcgaacct atgccggaga acgcattccg gcctatctca gcgcgaagaa aatgagcctg
    37261 catcagaaca gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca
    37321 catattgccc tcgtcgggct gcaacccgcg atgctcgacg actacggcgg cagcctgagc
    37381 gaactggcac gggagcaact gcccgctgcg gaacaatcgg cgctggcgca gttggctgcg
    37441 tggggaagtg tgccgcaacc ggctaatgaa tcgcgttgtc tcaattatga ctgtctgtcg
    37501 atggaaaatt acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga
    37561 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
    37621 cggtcagtga atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat
    37681 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
    37741 tactgcacga gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg
    37801 aagccatcgg cgatcgcttt ggcgcctttc gctttcctgc cactttagtg tttaccggcg
    37861 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
    37921 gcgggcttgt cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct
    37981 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg
    38041 tcaggtgaat gatgaaccga gtatggcggc cttggagcaa tgtgctcaca gcccgcaggt
    38101 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga
    38161 ggtgctggca gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct
    38221 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct gacgacagta aaagtgaaat
    38281 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
    38341 ggaggacaaa ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
    38401 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
    38461 gttggcgcat gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa
    38521 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
    38581 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaact ccacggggtt
    38641 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
    38701 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
    38761 gcagcggctt agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg
    38821 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
    38881 tcaggataaa gagggagatc taacattatc gggttatttt tctctcttcg cctacaggag
    38941 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
    39001 actttttatt tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca
    39061 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
    39121 tcggtatcaa ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
    39181 ctaactggtc attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg
    39241 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc
    39301 aacggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
    39361 tctctgcatt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
    39421 ttgatatcga caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg
    39481 tcagccaggt gaaatttgtg cacaccgtca tggcgggcta cgtgaccggg gccatgttta
    39541 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct
    39601 cgtttgccat cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag
    39661 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
    39721 agtggcaaac ggaacctgct cctgcaccgt tccatgtggt tgcctggccg gaacaggatc
    39781 aagagcgtaa cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact
    39841 cattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
    39901 aacgcggacg tatggcctgg ctgttaatgc aggaaatatc tcaaggcaat cgtgagccgc
    39961 atgtgttgca ggcattccgg gagctggaag gcgaccttgg ttacggcatg ttgctctccc
    40021 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
    40081 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
    40141 tgctgctact ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
    40201 atcgctgggt gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag
    40261 ccgggtggtt tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga
    40321 catactccgc gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
    40381 taggtcttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
    40441 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
    40501 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
    40561 ggtctttatg atctccgacg gatttgacat ggggatcggc tgtttgctgc cgctggtggc
    40621 gcgtaatgat gatgaacgcc ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa
    40681 tcaggtctgg ttgatcctcg caggtggggc attatttgcc gcctggccca gagtgtatgc
    40741 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg
    40801 tccgctggcc tttgattatc gcggaaaaat tgccgatgca cgctggcgta aaatgtggga
    40861 tgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
    40921 cttgttgctc ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag
    40981 cttctggcaa ctgctgacgc cattcccttt attgtgtggt ttgctcagcc ttgggatggt
    41041 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
    41101 acagctggcg actaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcaggtta
    41161 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
    41221 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga
    41281 atcgcccgtt ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat
    41341 ggcgatttat cgtggtcgcc cgggttgggg atttttgatg gcatcattga tgcaatttgg
    41401 cgtgattttc acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gtgtgagtcc
    41461 aatctccagc ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt
    41521 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
    41581 aatgtgggga cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg
    41641 agcaggaaca tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc
    41701 acccttgtgt tggtatggct ggacctgcgt ctgaaaagtt aacgaacgta ggcctgatgc
    41761 ggcgcattag catcgcatca ggcaatcaat aatgttagat atgaaaagcg gaaacatatc
    41821 gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc
    41881 attcgctcag agtgagccgg agctgaagct ggaaagtatg gtgattgtca gtcgtcatgg
    41941 tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    42001 aacttggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct
    42061 cggacattac caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc
    42121 gcagtctgct caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    42181 agccttcgcc gccgggctgg cacctgattg tgcaataacc gtacataccc aggcagatac
    42241 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataacgc
    42301 gaacgtgact gacgcgatcc tttgcagggc aggagggtca attgctgact ttaccgggca
    42361 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat ttcccgcaat caaacttgtg
    42421 ccttaaccgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    42481 caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac
    42541 ggagatattt ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac
    42601 cgattcacac cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttactaca
    42661 acgcacgccg gaggttgccc gcagtcgcgc caccccgtta ttggatttga tcatggcagc
    42721 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct
    42781 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    42841 gacgcttcca ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg
    42901 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    42961 gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    43021 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca
    43081 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    43141 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    43201 aaggcgttca cgccgcatcc ggtcactttc agttttactc tttctcggag taactataac
    43261 cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga
    43321 ttgcgccttt aatattaacg ccagcctgtt ccagacgctg cattgacaag ctcacctctt
    43381 tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccgacagag cgtcccacga
    43441 ccgcagcatc gctcaccgcc a
//
LOCUS       DADLXX010000021        43277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLXX010000021 DADLXX010000000
VERSION     DADLXX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.021"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            2..124
                     /locus_tag="JNX27_001113"
     ncRNA           2..124
                     /ncRNA_class="other"
                     /locus_tag="JNX27_001113"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            244..585
                     /locus_tag="JNX27_001114"
     CDS             244..585
                     /locus_tag="JNX27_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7882390.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(576..1502)
                     /locus_tag="JNX27_001115"
     CDS             complement(576..1502)
                     /locus_tag="JNX27_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7882391.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1592..2590
                     /locus_tag="JNX27_001116"
     CDS             1592..2590
                     /locus_tag="JNX27_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7882392.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2587..2805)
                     /locus_tag="JNX27_001117"
     CDS             complement(2587..2805)
                     /locus_tag="JNX27_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882393.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2807..4822)
                     /gene="ligA"
                     /locus_tag="JNX27_001118"
     CDS             complement(2807..4822)
                     /gene="ligA"
                     /locus_tag="JNX27_001118"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7882394.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGSTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQELMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4893..5780)
                     /gene="zipA"
                     /locus_tag="JNX27_001119"
     CDS             complement(4893..5780)
                     /gene="zipA"
                     /locus_tag="JNX27_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7882395.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            6010..6771
                     /gene="cysZ"
                     /locus_tag="JNX27_001120"
     CDS             6010..6771
                     /gene="cysZ"
                     /locus_tag="JNX27_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7882396.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6956..7927
                     /gene="cysK"
                     /locus_tag="JNX27_001121"
     CDS             6956..7927
                     /gene="cysK"
                     /locus_tag="JNX27_001121"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY7882397.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8311..8568
                     /gene="ptsH"
                     /locus_tag="JNX27_001122"
     CDS             8311..8568
                     /gene="ptsH"
                     /locus_tag="JNX27_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7882398.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8613..10340
                     /gene="ptsI"
                     /locus_tag="JNX27_001123"
     CDS             8613..10340
                     /gene="ptsI"
                     /locus_tag="JNX27_001123"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7882399.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10381..10890
                     /gene="crr"
                     /locus_tag="JNX27_001124"
     CDS             10381..10890
                     /gene="crr"
                     /locus_tag="JNX27_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7882400.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10932..11783)
                     /gene="pdxK"
                     /locus_tag="JNX27_001125"
                     /gene_synonym="thiJ"
     CDS             complement(10932..11783)
                     /gene="pdxK"
                     /locus_tag="JNX27_001125"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7882401.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11888..12262
                     /locus_tag="JNX27_001126"
     CDS             11888..12262
                     /locus_tag="JNX27_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7882402.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12295..13018
                     /locus_tag="JNX27_001127"
                     /pseudo
     CDS             12295..13018
                     /locus_tag="JNX27_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13222..14133)
                     /gene="cysM"
                     /locus_tag="JNX27_001128"
     CDS             complement(13222..14133)
                     /gene="cysM"
                     /locus_tag="JNX27_001128"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7882403.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14267..15364)
                     /gene="cysA"
                     /locus_tag="JNX27_001129"
     CDS             complement(14267..15364)
                     /gene="cysA"
                     /locus_tag="JNX27_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7882404.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15354..16229)
                     /gene="cysW"
                     /locus_tag="JNX27_001130"
     CDS             complement(15354..16229)
                     /gene="cysW"
                     /locus_tag="JNX27_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7882405.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16229..17062)
                     /gene="cysT"
                     /locus_tag="JNX27_001131"
     CDS             complement(16229..17062)
                     /gene="cysT"
                     /locus_tag="JNX27_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7882406.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17062..18078)
                     /locus_tag="JNX27_001132"
     CDS             complement(17062..18078)
                     /locus_tag="JNX27_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7882407.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18382..19173)
                     /gene="ucpA"
                     /locus_tag="JNX27_001133"
     CDS             complement(18382..19173)
                     /gene="ucpA"
                     /locus_tag="JNX27_001133"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7882408.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19360..20361)
                     /gene="murR"
                     /locus_tag="JNX27_001134"
     CDS             complement(19360..20361)
                     /gene="murR"
                     /locus_tag="JNX27_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7882409.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20324..21220
                     /gene="murQ"
                     /locus_tag="JNX27_001135"
     CDS             20324..21220
                     /gene="murQ"
                     /locus_tag="JNX27_001135"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7882410.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21224..22648
                     /gene="murP"
                     /locus_tag="JNX27_001136"
     CDS             21224..22648
                     /gene="murP"
                     /locus_tag="JNX27_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7882411.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22828..23727)
                     /gene="yfeX"
                     /locus_tag="JNX27_001137"
     CDS             complement(22828..23727)
                     /gene="yfeX"
                     /locus_tag="JNX27_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7882412.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(23823..24398)
                     /locus_tag="JNX27_001138"
     CDS             complement(23823..24398)
                     /locus_tag="JNX27_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7882413.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24459..24908)
                     /locus_tag="JNX27_001139"
     CDS             complement(24459..24908)
                     /locus_tag="JNX27_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7882414.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(24895..25320)
                     /locus_tag="JNX27_001140"
     CDS             complement(24895..25320)
                     /locus_tag="JNX27_001140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY7882415.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25534..26403
                     /gene="amiA"
                     /locus_tag="JNX27_001141"
     CDS             25534..26403
                     /gene="amiA"
                     /locus_tag="JNX27_001141"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7882416.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26407..27306
                     /gene="hemF"
                     /locus_tag="JNX27_001142"
     CDS             26407..27306
                     /gene="hemF"
                     /locus_tag="JNX27_001142"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7882417.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27312..28365)
                     /gene="eutR"
                     /locus_tag="JNX27_001143"
                     /pseudo
     CDS             complement(27312..28365)
                     /gene="eutR"
                     /locus_tag="JNX27_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28450..28911)
                     /gene="eutK"
                     /locus_tag="JNX27_001144"
     CDS             complement(28450..28911)
                     /gene="eutK"
                     /locus_tag="JNX27_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY7882418.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(28924..29583)
                     /gene="eutL"
                     /locus_tag="JNX27_001145"
     CDS             complement(28924..29583)
                     /gene="eutL"
                     /locus_tag="JNX27_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7882419.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29593..30480)
                     /locus_tag="JNX27_001146"
     CDS             complement(29593..30480)
                     /locus_tag="JNX27_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY7882420.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(30501..31862)
                     /locus_tag="JNX27_001147"
     CDS             complement(30501..31862)
                     /locus_tag="JNX27_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY7882421.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTSTVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            32051..33256
                     /locus_tag="JNX27_001148"
     CDS             32051..33256
                     /locus_tag="JNX27_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7882422.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWVNLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            33762..34478
                     /locus_tag="JNX27_001149"
     CDS             33762..34478
                     /locus_tag="JNX27_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7882423.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQNKAAAIAEF
                     KELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAEEDLRFDSKEEQLPEER
                     EQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLYTWMVSKCRYRGNDKYE
                     IWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGKCGKTYRLRIPSKGTAE
                     EASMTAQQHEREAKVSYLVH"
     gene            35529..36281
                     /locus_tag="JNX27_001150"
     CDS             35529..36281
                     /locus_tag="JNX27_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882424.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIYTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            37041..37436
                     /locus_tag="JNX27_001151"
     CDS             37041..37436
                     /locus_tag="JNX27_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7882425.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHKGKFKVTLTYQKACYIYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            37454..37717
                     /locus_tag="JNX27_001152"
     CDS             37454..37717
                     /locus_tag="JNX27_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882426.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            37740..38282
                     /locus_tag="JNX27_001153"
     CDS             37740..38282
                     /locus_tag="JNX27_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882427.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            38747..38953
                     /locus_tag="JNX27_001154"
     CDS             38747..38953
                     /locus_tag="JNX27_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882428.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            39412..39750
                     /locus_tag="JNX27_001155"
     CDS             39412..39750
                     /locus_tag="JNX27_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7882429.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            39731..40009
                     /locus_tag="JNX27_001156"
     CDS             39731..40009
                     /locus_tag="JNX27_001156"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882430.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            40002..41462
                     /locus_tag="JNX27_001157"
     CDS             40002..41462
                     /locus_tag="JNX27_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7882431.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            41484..42305
                     /gene="murI"
                     /locus_tag="JNX27_001158"
     CDS             41484..42305
                     /gene="murI"
                     /locus_tag="JNX27_001158"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7882432.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(42424..43118)
                     /locus_tag="JNX27_001159"
     CDS             complement(join(42424..42872,42872..43118))
                     /locus_tag="JNX27_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7882433.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
BASE COUNT        11020 a        11144 c        10552 g        10561 t
ORIGIN      
        1 ctatctgcga tactcaaaga ttcgaacctg cagcaggttt gagttgagcg cagcgaaaca
       61 acggagccgc tcgcggcgac ggcccgaagg gcgagcgaag cgagtcatcc tgcacgaccc
      121 accaatgcaa aaaagcgccc taaaggcgct tttttgctat ctgcgatttg taagattgat
      181 ttttcttctc aagttccttg ttcttctacc gatattacct cgtacagtga cgaggactga
      241 acaatggcca ctatctcttc aacttcaatt ccttccatac aaacacagag tagtaacagg
      301 gcatcacaag gtagcgatgt tgcttcacaa atcgcgcgga taagtcagca aatcatcaaa
      361 ctcactcaac aaattaaaga gattgtagac actagcggca gtgctgaaga caagcaaaaa
      421 caggccgaat tgattcaaca gcaaatcacc ctactggaaa cacaactggc gcagttgcag
      481 aaacagcagg cagaaaaggc gcaggagaaa gagcagcgtt tgtctctgaa tgtatccctt
      541 ctaaatcctg ttgaaaaaac gactcatatc gatatctata tctgatacaa cggatcccct
      601 tcccgaccag ccattagcgc attgccctgg tctcgtacca cgtcccataa cgcttcagcg
      661 gcggtggaaa gtgaacggtt cttcctgcgc accagcatca actggcgttc aaccaccggc
      721 gtaatgcgct taaccaccag tgggctacct tcaggcaatg gtagcgccag tgcaggaaga
      781 atactgatgc cgatgcctgc ggctaccatc gggaacagcg tcgccggatg tccaatctcc
      841 tgcacaatat tcgcctgaat accattgcgc gccagcgctg cgtcaatcag cggtcggctg
      901 cctgacgcgt aatcctgcaa caccaatttc gctccctgta atgcctgcca gggtacataa
      961 tcttccacgg ccaaagcgct atcgcgatgg caaagcagaa agaaaggctc ggaaagaatc
     1021 gcttcacatt gcagatcgcc cactgggcct ggatcgatga cgatgccaaa gtcgacatct
     1081 ccctggcgaa tactttccat cacccactgc tgcggacgat catgcaggac aaactgaata
     1141 tctggatagc ggcgatggct ttcagcaatg cattgcggaa taagatgcgc ggaaatggtc
     1201 tggctggcag cgacccgaac ttttccgctc agttgttgcc ccatacgccc ggtatcgcgc
     1261 aacgtgctgt taagttcatc cagcagtcgc tcaagacgca aagccaactg ctgccctgca
     1321 tctgtaagca ccacttcacg cgtggttctg tccagcaggc gaacaccagt atgattttcc
     1381 agctccttca cactgtgact tactgccgac tggctcaagc cgatacgctc tcctgcacga
     1441 ctaaaacttt tctcctgcgc tactgtgacg aaaaccttta attgttttaa agaataattc
     1501 atctgttttc ttcatgaatg gatgcaataa atcaatttta tttctcaaac tggaagaagc
     1561 acaatagaac catcgatcat ctggagtctt tatgaaactt tttcgtatcc tcgatccttt
     1621 caccttaacc ctgatcacgg tggtgttgct ggcctctttc tttccggcca gaggcgattt
     1681 cgtccccttc tttgaaaatc tgaccaccgc agctattgcc ctgctgttct ttatgcacgg
     1741 cgcgaagttg tcgcgtgagg cgattattgc tggcggtggt cactggcgac tgcatttgtg
     1801 ggtaatgtgc agcaccttcg tgctgtttcc gattctgggt gtactgtttg cctggtggaa
     1861 accggtaaat gtcgacccga tgctctactc cggttttctc tacttgtgca ttctcccggc
     1921 taccgtgcag tctgcaatcg ccttcacgtc aatggcgggc ggtaacgtcg cggcggcggt
     1981 ttgttctgcg tcagcatcca gcctgctggg gattttcctt tcaccattgc tggttggtct
     2041 ggtgatgaac gttcacggtg caaggggcag ccttgagcag gtcggtaaaa ttatgctgca
     2101 actgctgctg ccgtttgtgt tggggcatct ttcccggccg tggattggtg actgggtgtc
     2161 gcgcaataaa aaatggattg cgaaaactga ccagacgtcc attctgttgg tggtttatac
     2221 agcgttcagc gaagccgtcg ttaatggtat ctggcataaa gttggctggg gatcattgct
     2281 gtttatcgtg gtggtcagct gcgttcttct ggctatcgtg attgtagtta acgtctttat
     2341 ggcacgccga ctgggcttca ataaggcaga tgaaattact atcgtctttt gtggttcgaa
     2401 aaagagtctg gcaaatggca tcccgatggc aaacattctg ttccccacat cggtgatcgg
     2461 tatgatggtg ctgcccctga tgattttcca tcagatccaa ttgatggtct gtgcggtgct
     2521 ggcgcgtcga tacaaacgcc agaccgaaca gttacaggcg cagcaggaaa gcagcgccga
     2581 taaagcttaa agcggacgct tcaggggctg gaccagttgc gtcagcccct cggttttaat
     2641 cagcagcgtg atttgcatta gctcaccgag cttccctgcc gggaactcat ctttgcgggc
     2701 aaaccacagc agatactcct ccggcaggtc aattaagcga cgccctttgt atttgccaaa
     2761 cggcattatc gtattggcta tttcaatcag ctgctctttt tccatctcag ctacccagca
     2821 aacgcagcat ttccgcttcg tcgatgactt caatgcccag ttcctgcgcc ttcgccagtt
     2881 tagatcctgc agcttcaccc gctatcacca gatcggtttt cttcgacacg ctgcccgcga
     2941 ctttcgcgcc cagttcgacc agtcgagctt tagcgtcatc acgcgacatc tggcttaagc
     3001 tgcccgtaag caccacggtt ttaccagcaa acgggctgtc aatctcttcc gcgttgataa
     3061 cgatcggcgc aggccagtga acaccttccg ccaacagctc gctgatgaca ttgcggttgc
     3121 tttcttcggc aaagaagttg tgaacgtggg atgcaacgac aatgccaaca tcaggcacct
     3181 tttgcagctc ttcaatcgaa gcggcttcca gcgcttccag cgtgccgaaa tatgccgcca
     3241 gacctgctgc ggtggcctcg ccgacttcac ggatgccaag tgcatagagg aagcgagcaa
     3301 aggtggtttc tttcgctttt tccagcgcgt taaccacgtt ttgtgccaat tttggcccca
     3361 tacgctccag tccggtcagt ttgcctgcgg tgagtttgaa cagatctgcc ggagtatgga
     3421 catattcttt ttcaaccagc tgatcgatga ttttgtcgcc cattccgtca acatccatcg
     3481 cacggcggga aacaaagtgt ttcagcgact ctttacgctg cgcaccgcaa atcaggccac
     3541 cggtacagcg ggcaaccgct tcaccttcca cacgctcaac gtcagaacca cataccggac
     3601 aatgcgtcgg gaatacaacc tcacgggtat cttccgggcg ttcagaaagc acgacgttaa
     3661 ccacctgcgg gatcacgtcg ccagcgcggc gaatcaccac tttatcgcca atgcgtaaac
     3721 caagacgttc gatttcatcc gcattgtgta aggttgcgtt actcaccagc acgccagcaa
     3781 catggacagg ttccagacgc gcaacaggcg taatcgcgcc agtacgccca acctgaaact
     3841 cgacgtcacg cacaaaggtc atcagctcct gcgccgggaa tttaaacgct accgcccagc
     3901 gcggggcacg cgcgacaaag ccaagctgct cctgctgttc cagtgagttg accttaatca
     3961 ccacgccgtc gatatcaaag cccagcgtcg ggcggtcttc ttccactttg tgatagaacg
     4021 ccagcacttc ttccgccgat tcacaaagcg ttacccgatc gctgaccggc aacccccact
     4081 ttttaaattg cagtaaacgg ccaagatgag tatccggcag ctcgccacct tccagaacac
     4141 caacgccata gcagaaaaaa gtgagcggcc gcttcgctgt aatacgcgga tcaagctgac
     4201 gcagtgaacc agctgccgca ttacgtgggt tagcaaaaac tttcccaccc gtgcgtcgcg
     4261 catcttcgtt aatcttttcg aaccccgcct gcggcaggaa cacttcacca cgcacttcca
     4321 gacgcgccgg gatattctct ccgtgcagct tcagcggaat ggcgcgaata gtacgcacat
     4381 tagacgtgat atcttccccg gtggagccat cgccacgggt cgcggcactg actaaaacgc
     4441 cattttcata cagaatactg acggcaaggc catccagctt cagctcacag caccaggtga
     4501 ctttctcgtt gtttttcaga cggtcctgca cacgtttgtt gaaagcaaga aagctttctt
     4561 catcaaaaac gttatccagt gacagcattg gtacttcatg gcgtatctgg ctgaaagctg
     4621 ccagcggcgc agcacctaca cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt
     4681 tggtttccag ctcgcgcagt tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg
     4741 gcgcatccat cacatgataa agatattcat gatggcaaag cgtcgttcgc agttctgtca
     4801 gttgttgttc gattgattcc atatcgcacc atcaatgcta aaaacccccg acaagcgggg
     4861 gttcgaagag gagttaattt gccttaagtg tatcaggcgt tggcgtcttt gacttcgcgg
     4921 atgatgtcct ggtactcgcg caatttctgc ggagtcatca tacggcgctg atcgtcaagc
     4981 acgacaccgc ccacttcatc ggcaatatgc tgcgcagatt gcagcatcag cttgaagttc
     5041 tgcagctcgt caccgtaaga cggtacctgc ataaagatag tgacacccgg agtagtgaaa
     5101 tccttcattt caggatcaaa ggttcccggt ttcaccatat tcgccaggct gaataacgcc
     5161 gggccgctgc catccgggct aagatgacga tggtaaatat tcatatcgcc aaaaatgaag
     5221 cccgcttgtt gaatgctgtt aagaagcagt tcaccgttta gctcgctacc gtgatgcgcc
     5281 gcgacgttca tgataatcac cgcttctttg cgcttcggtt tatccataac tggagccggt
     5341 tccgctacag gctcaggctg tggtgccgct acgggttctg caggctggaa agcctgttgt
     5401 gccggttgcg gtgctgaatg cactggctgc ggcgctggcg cgacctgtgg cgaaactggc
     5461 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg gtgttgcggc
     5521 gacggacgag cagcctcatg ctcctgagcg ttagccgggg catgattcac gcggtgaaca
     5581 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
     5641 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
     5701 gtccagaaac cgtgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
     5761 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
     5821 catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca
     5881 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
     5941 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
     6001 atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg
     6061 caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg
     6121 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct cgatgtctgg
     6181 atcccgactt tcatgagtta cgttccggac tggctacaat ggctgagtta tctgttgtgg
     6241 cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac gattgctaac
     6301 tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg attgactggc
     6361 gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg aatcatgaaa
     6421 cgcgaatggc aaaaatttgt ctggtatctg ccgcgcgcaa ttgtattact aattctttac
     6481 ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg
     6541 atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag
     6601 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
     6661 ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt ttgtggcgcg
     6721 acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta acaatctacc
     6781 ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
     6841 ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt ccgcatattc
     6901 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
     6961 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
     7021 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
     7081 gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
     7141 cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct atgtagctgc
     7201 cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg aacgccgcaa
     7261 gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag gcatgaaagg
     7321 cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
     7381 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg
     7441 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
     7501 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
     7561 cgttgagcca accgattctc cagttatcgc ccaggtgctg gcaggtgaag agattaaacc
     7621 tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc tcgatctcaa
     7681 gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cccgtcgtct
     7741 aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg ccgcagcgtt
     7801 gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttattc taccatcatc
     7861 gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga aagaattgca
     7921 acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg
     7981 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
     8041 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
     8101 attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg
     8161 tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt cgcgtctgtt
     8221 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
     8281 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
     8341 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
     8401 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg
     8461 cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag
     8521 cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
     8581 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
     8641 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
     8701 aaatttctgc cgaccaggtt gctcaggaag ttgaacgttt tctgagcggt cgtgccaagg
     8761 catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag
     8821 aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca
     8881 tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa gttatcgaag
     8941 gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg
     9001 tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga
     9061 gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc gaaaccgcac
     9121 agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca
     9181 cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct
     9241 ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca
     9301 atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa
     9361 aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag
     9421 aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg
     9481 aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg ctgcccactg
     9541 aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg caggcggtta
     9601 tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag
     9661 aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc
     9721 tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg cgcattatgt
     9781 tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc gaaatctaca
     9841 aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg
     9901 tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta
     9961 gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga
    10021 tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg
    10081 atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac
    10141 gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta
    10201 tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag
    10261 agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg
    10321 aagaaaaaac aatctgctaa tccacgaaat gcggcccaat ttactgctta ggagaagatc
    10381 atgggtttgt tcgataaact gaaatctctg gtttccgacg acaaaaagga taccggaact
    10441 attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc
    10501 gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg taacaaaatg
    10561 gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc gttctctatc
    10621 gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa
    10681 ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc
    10741 attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt
    10801 atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt
    10861 gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc
    10921 ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct ctcatgctgc
    10981 tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcacagc atcgtttaac
    11041 gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc agtccctttc
    11101 aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact gaccaccacc
    11161 acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac ccattttaat
    11221 gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg gcaattttta
    11281 ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag cggcagtaaa
    11341 tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc aatatcgcca
    11401 atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc agtcagccac
    11461 tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac agcacgaagt
    11521 tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca ttcgtccgga
    11581 atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac cgtcggcacg
    11641 gcaaagacat tcaagccgtt ctgttttata gcaggcaccg caatgctgtt gcctacgctt
    11701 ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagcgccct actcttatcg
    11761 ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc tcatcgtatt
    11821 ccaaccgaaa ctttacctga ttctggcagt caaatcggct attacaaaac aaagataagg
    11881 taattcagtg aagaaaatca tttgtctggc cattacgctg ctaatgacac tccccgctta
    11941 cgcgaagtta actgttcatg aagaagcccg catcaacgcc atgctggagg gattagcaca
    12001 gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat acctgcgatg aagcggtttc
    12061 ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg agcagtttat
    12121 tgataaggta gcttcgtcgt catcgattac cgggaagccg tatatcgtga agatccccgg
    12181 taagagcgat gagaacgcac agcctttttt acatgcgtta attgcgcaga cggataaaac
    12241 ggtgcctgcg gaaggaaatt aatccgcttt ggtaaggcat ttacaggagg taacatgaaa
    12301 aaatgcttta tttatcacga tgaaaaatcg aataaatttt ggtggataga ttacgaaggg
    12361 gatagtttag ctgtcaacta tggcaaggta ggtagtattg gtaaattcca tacaaaagaa
    12421 ttcgataatg aagaacagtg tctgaaagaa gccagtaaat tgattgccgc aaaaatgaaa
    12481 aaaggatatc aagaaactcc agactttaac ttcatggatc tctactattt tgatgatgaa
    12541 gagattgggt tacatcttaa aacgtcacac ccaaacttcc agtgccattt tactgatcca
    12601 ctttatatgt gttgctggga tgaagaatct ccttttggta gcgatgaagg tgctgatgct
    12661 ctaaacgaac ttgaaaatag cctccgttct ggactgtgct gatttccctc aaatgttaat
    12721 tgaaactata tggggtatga aatacatcgc tatggacagt attcttgaag aggatgtgcg
    12781 tgcgcaatta ctagccgatg aaatgagctc tatccagagc aatatgatta cctacgcgac
    12841 tgcattcggt cagatcaaag tcatgggtaa aatctcccat aaacttaaaa agatgggact
    12901 caatgcacta gcgcgtcatc agcttaccgc aaaaattctt caatggggtg acggtcagga
    12961 ctcaccaata cttcaaaaaa tgattgatga ccttactgct tttccacacg aaaattaaaa
    13021 actacatttg tcggcagcaa caactgccga caaatcagat tattgacaaa gtgcactttg
    13081 tttatgccgg atgcggcgta aacgcctgat ccggcataaa aatattgcta attcaatata
    13141 ttgcagaaac attgtaggcc tgataagcgg agcgcatcag gcagttttgc gtttgtcatc
    13201 agtctccaat gctattaatc cttaaatccc tgccccctga ctaaaatgct cttccccaaa
    13261 cactccggtg gagaggtagc gatcgccacg atcgcagatg atcgctacca ccaccgcacc
    13321 agggttagct tttgccaccc gcagtgctcc agcaaccgcg ccgcctgagc tgacgccaca
    13381 gaatattcct tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat
    13441 atccagcacc tcatccacca gcgcagcgtt gaaaatcccc ggcagatatt ccgcaggcca
    13501 gcggcgaatg cccggaatac tgctgccttc ttccggttgc aggccaacaa tggtcaccgg
    13561 tttggattgt tcgcgcataa agcgtgaaac gccggtgata gtgccggtcg tccccatgct
    13621 ggagacaaaa tgagtgatgc gcccgccggt ttgctgccag atttccggcc cggtggtggt
    13681 gtaatgcgca taagggttat cgggattatt gaactgatcg agcagctttc cttcgccacg
    13741 attcgccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt tggtgacaag
    13801 aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca tgttgtcggg
    13861 catcagcaat ttcatgcgat agcctttcag cgcggcaatc attgccagcg caatgccggt
    13921 gttaccactg gtggcttcga ttaagacatc acccggttta atttccccgc gcttttccgc
    13981 ctcgacgatc atcgaaagtg ccgcacgatc tttcaccgaa cctgccgggt tattgccttc
    14041 cagttttaac cacacttcac tgccgttatc cggccccatt cgctgcaact tcaccagagg
    14101 cgtattgcct attgtttgtt ctaatgtact cacgatctct atccatacgt ggtgttgcct
    14161 gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc
    14221 cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg
    14281 cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac agccgcgcat
    14341 gttgcagacc aacgaataaa cgctcgccac gctgcggggc atcgtcgcca tgcatcacga
    14401 ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg tagtgacctt
    14461 tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gagactggta cggcggctga
    14521 tatccacttc ccacgggcgc aggaagagat ccaccggccc ctgatacgca ggtgtgtagc
    14581 ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc
    14641 ggttcacttc gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc catacctgat
    14701 tcggcgcatc agcctgttca atattcccct gactcatcac cactacacga tcggctactt
    14761 cggtcgcctc ttcctggtcg tgggtcacaa acacgctggt gaatttcagc tcttcatgca
    14821 gttgacgcag ccagcgacgc agctctttac gcacctgcgc atccagcgcg ccaaacggtt
    14881 catcaagcag cagaatttgc ggttctacag ccagcgcgcg cgccagcgcc acacgctgtt
    14941 tctggccgcc ggaaagctgc gccggataac gatccgccag atgcgcaagc tggaccattt
    15001 ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg cgacgcggca
    15061 gcaccgtcag gccaaaagtg atattgtcga ataccgtcat atggcggaac agcgcgtaat
    15121 gctggaacac gaaaccgact ttacgatcgc gcgcgtgcag gcgactcacg tcggtgccgt
    15181 ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatccgc agcagcgtgg
    15241 ttttcccgga accggacggc cccagcaacg cgaccatctg accagaagga atatccagtg
    15301 agatatcgtt cagcacctgg gtgcgaccaa aagacttctt gatattggca atctcaatgc
    15361 tcatgatgct cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca ctgcaacata
    15421 ctctttaaaa acagggtgat aatcgccatc agcgtcaaca gcgcggcggc ggtaaaggag
    15481 ccgacggtgt tgtagtcctg ctccaacaat tcaatctgta acggcagcga aagtgtttcg
    15541 ccgcgaatcg agccggaaac caccgacaca gcaccaaact cgccaatcgc gcgggcgttg
    15601 gtcagcacca cgccgtaaag cagcgcccag cgaatattcg gcaatgtgac gcgacggaac
    15661 atctgccagc cggacgcgcc aagcaaaatc gccgcttcgt cttcctggct gccctggctt
    15721 aacatcaccg gcaccagctc gcgcaccaca aacggacacg tcacaaagat ggtgaccagc
    15781 accattccgg gccaggagaa cataatttgc agattatgtt catccagcca gccgccgagc
    15841 gggccgttgg agccgtagaa cagcagatac accagaccgg caaccaccgg cgatacggca
    15901 aacggaatgt ccagtagcgt cagcagtaac tggcgtcccg ggaagttaaa gcgcgtcacc
    15961 agccaggcca gcagaatgcc gaacaccagg tttaccggta cggcaatcag cgcgatcatc
    16021 accgtcagcc agatggcgtg cagcatgtcc ggatcggcca gattctgtaa aaccggcatc
    16081 agccccttgc tgaatgcctg cacgaagatg taaatcatcg gcaccagcag gatgaacgcc
    16141 gaaaccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca
    16201 taacgcttca attgggtaac ttccgccatt aatgacctac cacacgccga ccaaagcgac
    16261 tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc accgaagcaa
    16321 tcgcgctcgc tgccgggtaa tcaaactcct gtaagcgcac aaaaatcatc agcgacgtca
    16381 cttccgtctt ccacgcgata tttccggcga taaaaatcac cgcgccaaat tcaccaagac
    16441 tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc ggcagcacca
    16501 ctttgcagaa actctgccag cgcgttgcac caagcgtttc cgccgcttct tcatattccg
    16561 ggcctaactc ttccagcacc ggctgcacgg tacgcaccac aaacggaatg ctggtaaagg
    16621 ccatcgccac cgcaatacca agccaggtat aggtgacttt gatatcaaac ttcgccagcc
    16681 attcaccgta aaaaccgttt acggaaaaga gcgaggccag cgttaaaccg gcgactgccg
    16741 ttggcagcgc aaagggtaaa tccatcagcg catcaagcag cgtgcggccc ggaaagcgat
    16801 aacgtgttaa aatccacgcc atcagcagac cgaaaacgcc gttaaaaatc gatgccacaa
    16861 acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg atcacctccc
    16921 agtactgcgc ccagctcatc tcagaaagct gcatcaccag cgcagagagc ggcagcagca
    16981 atatcaggca cacaaacagc agactggtgc caaggcttaa agtaaagccc ggcagcacgc
    17041 gtcttgaaga aactgcaaac attacttacg ccccgccgct aacagcttgt ctaactcgcc
    17101 gccgctggtg aagtgggttt tcatcacttc cggccatgag ccaaatttgt cttccacgcg
    17161 gaacagttcg gtctgcggga atttatcttt cagtttgtcc atgacttccg ggttattcac
    17221 acggtagtaa tagtcggtga tgatggtctg cgcctgtggg ctgtagagcc agttcaggta
    17281 ggcttttgcg gctttttccg taccgttggc ctgcacattt ttatcgaccc acgccaccgg
    17341 gaattccgcc agaatattgg ttttcgggat caccacttca aagccctgtg cttcatactg
    17401 tttacggatg ttgttcactt ccgattcaaa gctaatcagc acatcgccca agccacgctc
    17461 ggcaaaagtg gtggttgcgc cacgaccgcc ggtatcgaac acttcaacgt ttttcaggaa
    17521 ctgggtcata aactgttcgg ttttggcttc atcgccaccg tcagctttat ccgctgcgcc
    17581 ccatgccgcc agataggtat aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag
    17641 cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt tgcccttacg
    17701 caccaggaag cccatggtgg agtagaacgg cgagctatta ttcggcaggc gcgactgcca
    17761 gtcagccggg atcagcttgc ctttgtcgtg caggatttgt acgtcggtca cctggttata
    17821 agtgacaacg tctgctttta agccctgcaa aatcgccagc gcctgttttg atgacccggc
    17881 atgagattgt tttatcgtca gtttgtcgcc gccgttatct tttgcccatt gctgttcaaa
    17941 cggcggattc agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc
    18001 cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg agttcttttt
    18061 cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat
    18121 atttataacg gacgtgaaag gagtaacgat tttatatacc gtttggtgat ttggaagtta
    18181 aaaagggaat aagaacttgg ttcaacaggc gtaattgttg caggcagttt ggacacagac
    18241 agcgcggaga aaccggagcg tacacatagt acgtgaggat ttcgagcact gcccggggcc
    18301 aaaatgacaa atgaaatagc ctggtgaact tagttcaaga cgaaatcctc cccacacatg
    18361 cagggaggaa acagaggtga atcagatacc gacgctaacc gtctccggca gtgtgctgcc
    18421 gccatcaatc acattctgtg taccggttaa atagctggat tcatccgatg cgaggaaggc
    18481 cgccagttcg ccgacttcca gcggatcggc gaggcgacac agcgggattg cttttgccat
    18541 ttcagtcagc accgattctg gatcttccgg gttcgactgg cgggcaatgc tttccgccat
    18601 tggcgtgcgg acgtatcccg ggcagatggc gttaacgcga ataccggatt gcgcgtactc
    18661 caccgccagc gatttagtca ggccaacaat cgccgctttc gttaaggcat acgccgtttc
    18721 gccaggatcg gccaccatat caccagtgac tgaagacatc atcacaatgc gaccatcttt
    18781 gcgcgcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt taatattgat
    18841 atcaatatgg aaatcgcgat cttcatcgct catatcgagg aaactgccca gacgacaaac
    18901 gcctgcgtta ttcactagga tatcaatgcg cccttctttt tccttcgcgc gtttgatagc
    18961 tgcggctacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc gatgaccacg
    19021 accacacagt tcgtccgcta gcttttcgat ctcaggggag atatccagca agattaggtt
    19081 cgcgccatga cgtgcaaaag ttctggcaat tccttcgcca attccctgca atgcgcccgt
    19141 aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt taatacagag
    19201 cgttacactc cattccctct gaaaatacag gataaacaaa atattgctgc ggatcagctc
    19261 actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag gcgttgtgtt
    19321 agctcactgc tgcgctgaat cattttcaat gacgccacat cattgatgtt gcacaagacc
    19381 aacaaacagt aaatcggtaa cagaattttg cgccgtgcgg gtagacatcg acgagctacg
    19441 ccattcggtt tcaccggaaa cggtatcgag cgtgaaatgc gccagccgcc gcagcggaga
    19501 gtcagccaga gaagtaatgg caatcaccgt tgccccctgc tttcgtgccg cttctgcgca
    19561 caagacaatc tcttttttac tgccgctgta agagatagcg atttgtacat cgcctttttt
    19621 caacgcctgg gaaacggtgg cttgtacgtg cgtgtcggct tcgcaggcca cgcgatagcc
    19681 aattttcatc agtttgaacg acagatcgcg ccccaccagc gccgatccgc ccaggcccgt
    19741 tatctggata aacggtgctt tactgatgac ctcaatgatt ttttgcagcc gcgcgtagtc
    19801 gaacaacgca caggtttgct ccagcgccag ctctttttcg cgattcagtt tgcgggcaat
    19861 cacttccagc gaatcatcac tggtaatcga actgtgcagg tgcagcgccg tggcattcgt
    19921 tttctcccgg ctggcactgt attcgccaat taacgccata cgcaattcag taaatccttg
    19981 cgcgccgagc ttctgggcaa atttcacaat gctcgactgg ctgataccga gctgtttcgc
    20041 catctgccgt gaagagacgg atttcagctc gctcactctg gcccgcagaa agtcagcaat
    20101 tttttgctcg ttcccggtaa attcactttc cgcattgcgt attttcgtca ggtacaacat
    20161 cttccactct cctgggctgg cagcaaaaat tccgcttaag ttcttatgac gctcttcaca
    20221 ctctgcgagt gtagactcaa tgattccttt aagtctgtca attcgggata tatgattcca
    20281 tacatctgca attatttgat cgtaaatagt aaggtcacca ccgatgcaac tagaaaagat
    20341 gattactgaa ggctcgaaca ccgcctcggc tgaaattgac cgcgtatcga cgctggaaat
    20401 gtgccggatt atcaacgatg aagataaaac cgtaccgctt gccgttgatc gcgtcctgcc
    20461 ggatatcgcc gcggcgatcg atgttatcca cgcccaggtc agcggcggcg gtcgtctgat
    20521 ttatctcggt gcgggaacat ccggtcgtct ggggattctg gatgccagcg aatgtccgcc
    20581 cacctacggc gtgaaaccgg gtctggtggt tggtttgatt gctggaggcg aatatgccat
    20641 tcagcacgcg gtggaaggcg ctgaagatag ccgggaaggt ggtgttaacg atctgaaaaa
    20701 tattaattta acggcacagg atgtggtggt tggcattgct gccagcggtc gcacgccgta
    20761 tgtgattgcc ggactggaat acgcacgcca gctcggctgc cgcacagtgg gaatttcctg
    20821 taatccgggg agcgccgttt caaccaccgc tgagtttgcc attacaccga ttgtaggtgc
    20881 cgaagttgtt accggttctt cgcggatgaa agcaggcaca gcgcagaaac tggtgctcaa
    20941 tatgctttcc accgggctga tgattaaatc cggcaaagta ttcggcaacc tgatggtcga
    21001 tgtggtcgcc accaacgaaa aactgcatgt gcgccaggtc aatatcgtta aaaacgccac
    21061 cggatgtaat gccgaacaag cggaagcggc gttaattgcc tgcgagcgca actgtaaaac
    21121 ggccattgtg atggtgctga aaaatctcga tgccgcagaa gctaaaaaac gcctggatca
    21181 acacggcggc tttattcgtc aggttttaga caaggaataa cccatggcca aagagatcag
    21241 cagtgaactt ctgaacacca ttcttacccg tgtcggcgga ccgggaaata tcgccagttg
    21301 tggtaactgt atgacgcgcc tgcgtctggg tgtacatgac agttcactgg ttgatcccaa
    21361 tatcaaaacg ctggaaggcg tgaagggcgt cattttgacc agcgaccagg tgcaggtcgt
    21421 ttttgggcct ggtaaagcgc atcgtgccgc aaaagcgatg agcgagctgc tgggcgaggc
    21481 accggtacag gatgccgcag aaatcgccgc ccagaacaaa cgtcagttaa aagccaaaca
    21541 aacctccggc gtgcaacaat ttctcgccaa attcgccacc atcttcacgc cgctgatccc
    21601 cggttttatt gccgccggtc tgctgctggg gatagcgacg ttaattgcca cggtgatgca
    21661 cgttccggca gatgctcagg gcacgctacc cgacgcgctg aattttatga aggtgttcag
    21721 caaaggtttg ttcaccttcc tagtgattct ggtgggctat aacgccgccc aggcatttgg
    21781 cggcacgggc gtcaatggcg caattattgc cgcactcttt ttgctcggtt ataaccccgc
    21841 tgcaaccacc ggttactacg ccggttttca cgatttcttt ggtctgccca tcgatccgcg
    21901 cggcaatatt atcggcgtgc tgattgccgc gagggcctgc gcccgcattg aaggcatggt
    21961 gcgccgcttt atgccggacg atctcgacat gctgctgacc tcgttaatca ccctgctgat
    22021 caccgctacg cttgcgtacc tgatcattat gccgctgggt ggctggttat tcgaaggtat
    22081 gtcatggctg tttatgcacc tgaacagtaa tccgttcggt tgtgcggttt tagccgggct
    22141 gttcctgatc gccgtggtat ttggcgtgca tcagggcttt attcctgttt acctcgcgtt
    22201 aatggacagc cagggattca acagcttatt tcccatcctt tcaatggcag gcgcgggcca
    22261 ggtgggcgcg gcgctggcac tctactggcg ggcgcaaccg cacagtgcgc tacgcagtca
    22321 ggtacgcggg gcgattattc ccggcctgct gggcgttggt gaaccgctga tttacggtgt
    22381 caccctgccc cgcatgaagc cgtttgttac cgcctgttta ggcggcgcgg cgggcggttt
    22441 gtttatcgga ttgatagcct ggtggggtct gccgatgggc ttaaacagcg cctttgggcc
    22501 gtctggtctg gtggcgctgc cgctgatgac ttccgcacaa ggcatccttc cggcaatggc
    22561 ggtttatgct ggcgggattc tggtggcatg ggtttgcggg tttattttca ccacgctctt
    22621 tggctgccgt aacgtcaatc tggactgata ttatgaaacg gacaatgctc tatctttctc
    22681 tgctggctgt tagttgtagc gtcagcgccg caaaataccc tgttctgaca gaaggtagtt
    22741 gatcaagtat atgcggcgtt aaagcagtaa taaaaaggcc agtcagtaat gttactgcct
    22801 ggcctttaat caatgaatca gaaacgctta cagcaccatc aacttgtcca gcgacggtgc
    22861 gaaataatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac gtttgccatc
    22921 ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg cgcagtaggc
    22981 gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct ggcgaacaat
    23041 cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg tttccggacg
    23101 ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca tctcctgatc
    23161 gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca caaacacata
    23221 gctgccgccg gcatccacgc cgtctttgat aaccgccact tcgcggcgcg tctcttcacc
    23281 cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca cccaacggaa
    23341 gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg ccgcctgagc
    23401 gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat cgaactgggt
    23461 cgtcggcgca aggcctttac cgtagcccgg aaaatctttc agctcttctg ccccaacgcc
    23521 gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa gatgcgcgtc
    23581 cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg ccgcacgcag
    23641 ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc aatgttctgg
    23701 caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataagaatg ccacgcaatc
    23761 cgtggcattg ttgagcgcta ttttacgtgt tttttcgcga cggcggtttg ttcagacgca
    23821 aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc gtccgaaggc
    23881 attaaccctt caggaccacg ccattccccg ctgaactggt agctaatatg ttgactgcct
    23941 tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca attgccaaat
    24001 gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc agcaggaata
    24061 tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat caccatcgcc
    24121 acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc ggttttcatt
    24181 ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc ttgcagtggt
    24241 gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggacga tccaaaccag
    24301 ttccacgggt tagcggcaga ccagttaact gacgacatcg tggaacagcc ggtcagcatc
    24361 aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt ggttattcaa
    24421 taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc ttcattaaaa
    24481 caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc cgccactacc
    24541 agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg ttgccagcaa
    24601 agtaaaacca cctgcgccaa cagcaacaga aaatagagca catgccaggt gcgaggaaac
    24661 gaagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaataccggg aatcagcccc
    24721 agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc ctgctcgcgc
    24781 gacgcaccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag cacaagccag
    24841 aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg gaactcagta
    24901 ctcttcatct tcaatcaaac gcttacccag actaagcacg tcggcgtgtt catatcccag
    24961 gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct gaattttcgg
    25021 gcagccgcga gcaatcagct ttttctccag ccggttaagc aacgcattgg caattccacg
    25081 cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc cgtcataacc
    25141 gcccatcacc gttccgacca cttcaccgtt tacctcagcg accaaaaaca aactgacgtc
    25201 atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggactca gcaagtcgca
    25261 acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc gtatctccat
    25321 ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg tcgaattagc
    25381 caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca actattgaaa
    25441 tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga tacgatataa
    25501 catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc actaaaaaca
    25561 ctcacttcgc gccgccaggt gctgaaagcc ggtttagctg ccctgacgtt gtcaggaatg
    25621 tcgcaagcca ttgccaaaga cgaaccttta aaaaccagca acggacacag caagccgaaa
    25681 gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg cggaattgat
    25741 accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc gattgctaaa
    25801 aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg ttctggcgat
    25861 acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc agatctgttt
    25921 atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc ggtatttgcc
    25981 ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg cgaaaaccgc
    26041 gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca gcaagtactg
    26101 tttgatctgg tgcaaaccga tactattaaa aacagcctga cgcttggctc gcatattctg
    26161 aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc cgcgtttgtg
    26221 gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat caccaacccg
    26281 gaagaagagc ggctgttagg cacggcggcg tttcgtcaga aaatcgccac agcgattgct
    26341 gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc gaaaaagcga
    26401 taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct tcaggatacg
    26461 atttgtcagc agctgaccgc cgtcgatggc gcagaatttg tcgaagatag ttggcagcgc
    26521 gaagctggcg gcggcgggcg cagtcgggtg ttgcgtaatg gtggtgtttt cgaacaggcg
    26581 ggcgtcaact tttctcacgt ccacggtgag gcgatgcctg cttccgccac cgctcatcgc
    26641 ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt gcatccgcat
    26701 aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt ttattgccga aaaaccgggt
    26761 gcagagcccg tttggtggtt tggcggcggc ttcgatttaa cccctttcta tggttttgaa
    26821 gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gccagccatt tggtgaagac
    26881 gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca tcgcaacgaa
    26941 cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt cgaccactgt
    27001 tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc aattgtagag
    27061 cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta ccgtcgcggt
    27121 cgttatgtcg agttcaatct ggtctgggat cgcggcacgc tgtttggcct gcaaactggc
    27181 gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga gtatgattat
    27241 cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt cagggattgg
    27301 gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca acgtcaacga cggcttctcg
    27361 gcaaacagct gctggtaatc cgtggcaaat tgccccagat gccagaatcc ccactgcatg
    27421 gcggcgtctt ttaccgttgt gctttgcgac cacgggctta tcagttcgcg gcgtacggcg
    27481 ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc gtgaaacgcg
    27541 ttttgtagcg tgcggcgact gacatgcagt tgattacaca aatccagcac cgtcaccggt
    27601 tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg gtaactctga
    27661 tgactgatgc tttccgccgt caccattggt tgcgcttctt ccagcatcgc ccccatcgcc
    27721 attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt ttccgggttc
    27781 tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt atgctgcttc
    27841 tttcacttcc agcgccgact ggctacgcag catatgtaat acccgatccg gattgtgcaa
    27901 aaagttagcc tgacaggtga tgacatcttc agacagcact acgcccagga tcgtataatc
    27961 gtccggcgta cttaattcaa actcggttcc accggggcgc gttgctattt ctgcgcttcc
    28021 cagacattgc gaaccgataa atccctgctc accgcgcgtc gccggaatgc caaaccagaa
    28081 cgagttcggc cagaccaggc acgactgacg cagcgccaga ccggtgtatt cacgaaaaac
    28141 ctgaatatca tcgagtagaa tttccgtaaa ttcaccatga aacttgcccg gatgcagctg
    28201 atcgtaaatc tgctgccagg cggtaatcgt caaagcatgt tcatagacat ccgttgtctg
    28261 tcgttgatga acattatcca cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg
    28321 ttcatgataa agatggtgca aattggctgt acgggtcttt ttcatgatgt taatgccggg
    28381 tgttgtagga cacccggcac ctccgacagg ttaatggggc ttgagacgat aacgactact
    28441 gcgtttacgt tacgtcccgg cagaaaagag ctgttcgagc gcatttctgg ctttttccag
    28501 cggccagcca aagtgggcgg ccacttctcc tgccgtcatt ccctgacata ctgatgttaa
    28561 cagcgccaac aactcgtcag cagattccgc gacgataact ggcgcgtcgg gttcctttac
    28621 gggttgcttc ggctggcggt taaagccagt aaccagccac tgggtgtcat cgtctggccg
    28681 accgatctcc ttacggctga tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc
    28741 cagcgccgca cgacacgccg ccagatcgcc ttccaccacc agcgttaagc gaccagggtc
    28801 aagcacttcg tgactgagca gacgaacgtt agctgcttta agcatggcat ccgctgcatc
    28861 tatcgcggcg accatgccgt ccacttccag caatcccagt gcattgatca tcggcaacct
    28921 ccgttacgca cgctggattg gattacgcgc gatttccagc actgcatcgg taaaggcgtt
    28981 acaggctgct ttacacgcgg cctggctacc ggttaaaaat gctgccgagt agttggtttc
    29041 agacggtggc gggacgtagg tcaccagctg tacgtcggca gatttcaacg cggcatcaat
    29101 gccgtaggtc gcttccagcg gcggtgccac cagatacgcc atcggatcgc caagcgtgat
    29161 cccggcggtt gatgaaagat aagaacctgt acgcgaaacc acatgcgcca ggaaggcggt
    29221 gttttctgcg tcattggccc actggaaagc tgcgccattt tcaatatgtg caaccatcgc
    29281 atccagaccg gcacgcactt ccgccgggtt tggcccacca agcataatca gcacttcacc
    29341 ggcagtcggt gacgggccgt gtgccgctcc cgcatacagc gagcgaccat ataccacttc
    29401 gaccatcgct tgtttggtcg cttcgtcagc cgcaatgtag gttacgtcgt cagaatcggc
    29461 agaaatcagc ccgaggctac gaatatgcgg cggcaatttt agttcacgcg caaattcagc
    29521 gttaacggag gcaatcaccc gcatggcggt taccgacggt cgaatcaaat ctaaagctgg
    29581 catgatgcct ccttatcggg tcatgttgat gccggatgct ttctgctcca gcatacgttt
    29641 cgccaaatcc acaatgacgg ctgcggcttc aaccggcggc gtgccgccct ggtgaatgtt
    29701 agagatacag gtgcgatcgg cctcaacggt ggtcgccata cgcggcgagt aaacggcgta
    29761 gcaggagagg ctttctgact gccctaaccc cggacgttcc cccaccagca ggatcaccac
    29821 tttcgcgccg aggatctcac cgatctgatc ttcaatcttc acgcgaccat aacgaacaaa
    29881 gaatggcgtg ccgactttca gcccggcctg tttcaggccc gccatcagcg gcggcaggat
    29941 ctcttcgtag ttgacggtga tggcatcagt tgacaggcca tcagaaataa cgacctgtac
    30001 gtccggatta gcaacacact gtgctttcag cgcttcaaca gcttctgcac acaggcggcg
    30061 gcccatatcc gggcgagtca ggtagagatt tttgtcgcta atttcagagc gtacttccag
    30121 caagccctgc gctttcaccc actcttccgg cacttctttc agtacggtat ctttcgaacg
    30181 agagtgatcg gccaagaaac gcagcagcgc ctgagtacgt ggacgcggac cggcacgacc
    30241 ggtacaaacg cgggccacgg tgctgcggcg cagttctgtt aatacgtctg cgcgatgcgg
    30301 gttttcaaca ccaatccacg cttttgcttc agcggaacct aaatccagcg cacagctttc
    30361 cgaggtcacc ggtgccgcac agttggtggt ggcgcacttt gcttctgacg gcgcgggggc
    30421 cgcttgtccc attgacgcca tcacgctgcg tacaatttct tcaatctgtt tttgatccat
    30481 gatgtgttat ctccgcgtca tcagaagaac agtgacggat cgcccgcccg tttggtcagg
    30541 cgaccgtttg ccataatgcc catgctttcc agccagcgtt caaactctgg tgacgggcgc
    30601 aggttgagta actgacgcac agtggaggta tcgtggaatg cggtggtctg gtagttgagc
    30661 atgatgtcat cacccagcgg catccccatg atgtagttgc agcctgcggt ggcgagcagg
    30721 atcatcaggt tttcgttgag gttctggtca gagtcagcgt ggttggtgta gcagccgtca
    30781 cagcccatag agatgccgct cagcttgccc ataaagtgat cttctaagcc agcacggata
    30841 atctggcggt cgttgtagag atactccggc ccaataaagc cgaccacggt gttgacgata
    30901 aacggatcgt aatgacgcgc cagcccatag ttacgtgctt ccatcgtcac ctggtcagcg
    30961 ccgaagttag cgccagcgga tagcgcagag ccttgtccgg tttcgaagta gaggcagttt
    31021 tccccggcga tacgattgaa ctctgcgccc actgcgcgcg cttcgtcgag catcgccagt
    31081 tccacgccaa actctttcag ccctttttcg ctgccacaga tactctggaa aatcagtccg
    31141 ccaggagcgc cgcgacgaat cgcttcgatc tgggtggtga cgtgcgccag tacgcagccc
    31201 tgagttggga tgttgaattt gtcgatcacg ccataaatgg tatccagcac gcggcttaag
    31261 ttttccacgt cgtcagttac cgggttaacg ccgatcaccg catcgcccac cccgaaggaa
    31321 agcccttcat agatttgcgc agcgatactt tgcacgtcgt cacgggtatc gttcggctgc
    31381 aaacgggcgc taaaggtgcc cggaataccg atggtggtat tggccttttt gattaccggc
    31441 attttcttcg cgccgtagat caggtccgcg ttggagcaaa tcttcgctat cgccgcgacc
    31501 acttccgagg tcagcccttt gcgggtaaag gcaatgtcgt ccacgctggt ttcatcgctc
    31561 agcacatact cacgcagttc gctgatgctc cagtttttaa tctggttgta ggccgtttcg
    31621 ttaacgtcgt cctgaatcag ccgcgtcacg cagtcatctt cataggcaat caccggatta
    31681 ttgcggatgt ccgctacggt catttccgac aacacctgct ttgccgccac gcgctcctgt
    31741 gagcttgccg ctgcaacgcc cgccagcaca tcccccgaac gcagttcgtt ggctttagcc
    31801 agcacctctt ttacatcctt aaactgatat acattgccga acaatgtggt ctttagtttc
    31861 ataagtcgtt ccctcaggaa gaagtgatga gtaacatttc tcacagcctg caaacaattc
    31921 tatttcatcg ttagcccttg tcatgtaagg tgatttcctt aacacgcccg ttacttatcc
    31981 tgccgaacgc aaccgaactc gccggataca tgagcggggt tatcgagtcg ttagatcgag
    32041 gtatccggtt ttgagcaaaa caccactcac agcaaaggcc atagacgccg cacaaccaaa
    32101 ggacaagcaa tacaaactca ccgcttcact gacgcccggc ctgtttttgc tggtgcatcc
    32161 caacggcagc aaatactggc gattccggta ctggatagac aagaaagagc ggttacaggc
    32221 tattggggta tatccgctga ttagcctcaa ggaagcccgc aaacgcgcca cagagagccg
    32281 tttactgata gcccagggaa ttgacccaat ggatttggca cgcaaggaga aagccattga
    32341 tgcactcaac atggcggcag gttttaagac ggtagcggag gactggctgg ctaccagggt
    32401 aggcggctgg agtgagagct atacaaagca ggtcaaatca gcactggaga aagacgttta
    32461 tccggtactt ggcaagcgtt caatcgttga tataaccgcc cgtgacgtgc tggcattact
    32521 ccagaagaaa gaacgtaccg caccggagca ggcccgcaag ctacgccagc gcatcggtga
    32581 gatcttcaag tttgccgtta tcaccgaact ggtaacgagg aatccggttg cagatcttga
    32641 tacggcactg aaggctcgcc gacctggtca caacgcgtgg ataccgatta gtgaaattcc
    32701 ggccttctac aaagcccttg agagggccgg gagcgtccag attcaggcgg caatacgttt
    32761 gcttatatta acggcattaa ggaccgcaga actgcgtttg tgccgttggg aatgggtaaa
    32821 cctggaggac gcaacaatca cattgcccgc tgaagtcatg aaggcccgcc gcccgcatgt
    32881 ggtcccgcta tcacggcagg cggtcgagct attgcaggac cagttcacac gcagcggata
    32941 cagtgttttc gtgttcccgg gccgattcat ggataagcca ttatcagcca gcgcgatact
    33001 caaggcgctt gagcgtatcg gatacaagtc aatagccaca ggccacggct ggcgcacaac
    33061 gttcagcacc gcacttaacg aaagcggcag gtataaccct gattggattg agatccaact
    33121 agcccacgtc ccgaagggaa ttaggggagt ttacaaccaa gcggcctatc tcaaaatgcg
    33181 ccgtcaaatg cttcaggact acgccgacgc gattgattcg atactggctg atggtatcaa
    33241 tccgcttgaa ctgtaatgaa tgattaatca cgttttccga cgactccacc ggagtaggcc
    33301 ggataaacac tggacacctt caaaataact attcactttt gccggattcg tccggctttt
    33361 tttgtctttg ccgatgggat caattattcc aattattcca attattccaa ttattccaat
    33421 tattccagta ttgttggaat aaataaatat caatgactta aagccgtgtt tgcaacaggg
    33481 tggatcgggg atcgaaaata ggatcaacac aaaaacaccc tggaggctac agcacactag
    33541 ggggacacaa aaacaacaca ggaaaaaata ccatgtacca atcagcaacc gcctacgtgg
    33601 cgaaaccagg cgaaacactg gtcaaacaca aaattgcaac actccaagca cttcaaacac
    33661 tccaagccct tccggaacag gttcaggccg cctttcgtga tggcatgaac atcatccgct
    33721 tcgatgggta tcgctgggaa tacaagccag caactggatt cgtgcgcata gatcaaatct
    33781 tgtcgccagc caatgaagag ccagcgctag ccaatgcaga accagcgcag gagcaggagc
    33841 aggagcagga acaggaacag gaacagaata aagcggcggc tattgccgag ttcaaggagc
    33901 tgcaaagaca acaaatcaca gcgcagcaag aagaatgccg ccacccgctg gcaatgccca
    33961 agccagtagc gaaagccaac acagacaagc cgacttcatg gtttaacgaa gaagcggaag
    34021 aagatttgcg ttttgatagt aaagaggagc agttgcctga agagcgggaa caggtggaaa
    34081 cggaagacca gcaaaacgat tatcgcaacc ttccagccgt caataacgct atggcttacc
    34141 tcttcaaaag cgggctgttt cacaagctga aaggcgatga gttaaagcta tatacgtgga
    34201 tggtatccaa gtgcaggtat cgcggtaacg acaaatatga gatttggtat tcccgcacca
    34261 aaatggcaaa agcgttaggt aagagcgttg acacgataga tcgccacctg gaagcgttga
    34321 gaaagctggg acttatccag gaaatgcgcc aggcactgag gggcaaatgt ggaaaaacct
    34381 acagactgcg aatcccgtca aaaggcacag cagaagaggc ctcaatgacg gcacagcagc
    34441 acgaaagaga agcaaaagtc agctacctgg tacactaggc agcgggtcag caaagggggg
    34501 caaatgcccc ttttttcatg tcagataatc agatcattac ctcaataaac taataatcac
    34561 atcgcggtta caaccagttt gatagtgata gctcaaaccc agacgtggcg ctggctgcaa
    34621 gcaaaataag ccgcaaaaat gcggtcctat ttgccagaac cttttattat tcaattggtt
    34681 agtgcaagaa aaagcgtgat tactgttcaa gaagtatcca gaaaggtcat gattttgcca
    34741 tgattttgac gctgaaaaaa aataggtggg catttttgcc cactcactag ccgcaaattt
    34801 gcccactcac tagccgcatt tttgcggccc aaagtaataa atacagagaa ataataaata
    34861 cagatcagcg ccaagatttt tcgtcttttc ggcggaatca agagactagt gcttacgtcc
    34921 tgataacctc cagagcagac cttaacttct gtgcagcagg actacgctca ttgccgctcg
    34981 gctaggtcaa atcctcacga taaagctgtt cggattgtga ccataatgtt aaaggactct
    35041 ttaaatcatt ccttgacgaa ttaactagct ggcttaagcg gaatagcatg caggataaaa
    35101 acaaaaccag cttagcagtg gggagagctt cgcttcgctc gtctcctggg gccaatgctc
    35161 aatgaaacga cgtaaggcac ggagcgctca ccataggtag acacataaac atctgagagc
    35221 gcaccttcta tcgcgctacg cgctcaataa aaaccaacga caccgccaca ctgcaagacc
    35281 aatggttttt atcgcgtata cagaccagac aggaaacagc agcaaagcag cgtatagcca
    35341 ggtgcagaca gcacactcac acgacgcaca gcaggcttaa gtgatcactc aggaaacttt
    35401 atccccgccg ccgatcaccg cgaactatca tgaccgcgcc aaattcgaaa ggaatggccc
    35461 atagaacgcg atgaacacac ataagcgcta acttgcttac ctgaaaactt aacgtcgcca
    35521 cagagcgatt gagagcgctt aaaatcgatg cgatttcatt aaatttttct cactcgcctg
    35581 aatggtggcg atcaggactg atcgcaacat tgccccgcct cgatattgag cgcataatat
    35641 acacatacat taagcggaaa agagataagt ttattgtgaa aatcaggggg ttacacatca
    35701 tgcgcatatc agaacaacaa cggcagatcc tcgactatct ggcaaaaaac gaatcaatca
    35761 ccgcacgaca agccgcagat ctcgtttatg gtggcaatgt tacccgcgtc caggttgacg
    35821 ccgcccgccg ctcactcatg acgatggtta gtaatgggct aatcaggaaa caaggccgcg
    35881 ctttcgtagc tgaaagacat atcaatcacg cttatagcca ggacgttgaa gaattgcgcc
    35941 gccagatcct ttgggtattc agcaaacagg gtcaggagta tttcctttct gaacacccaa
    36001 atttcgccgc caaagagtta acagtaagcg gattgtcgtg cgcactggat tatcttgtcg
    36061 gtgaaggttg cgttacttac aagcagctat ggcacgccct gaatacgctt gtagaagatg
    36121 gtttactcat tgccacgcgt gagaacatcg aatactggat aatgccgcgt tcaggtcgcc
    36181 gcaatcatgc acggtatatt tcaggcaaca gatacagaac gccggacttt aatccgacac
    36241 aggaagatcg ggaccgtgcc gaaatgatgt gcagggaata ggagaaaaga attatggata
    36301 agttattttc gatgcagtga acacaagcgg gcgcaatgcc cgtttttatt gccttattcc
    36361 acgcctgacg aatcaatttg atcacacctg agcaaatgca tagccagaag ttaaggccgt
    36421 tatattgcga ttgggagcgt tttaaacggt atcaatttaa ttaattctga tgtggtgatt
    36481 tgcaggcaaa aaaaacccgc cggagaagcg ggaaatagag acatatcgtt gtagttatta
    36541 acagatcact ttgaggtcga gggcggctaa ccctcgctaa tattatcccg ccattacctt
    36601 gcgggtgcaa ataatctttt attgctttga tgatttaata aatagcactt tttgctaaca
    36661 aaaaccgctg tcaatatatg gccaaaaatg tcaacagaaa aaataaggtt tataatatta
    36721 agtatcacat gggtaatggg ttcagggaat cggaatccac cgcctcacgg aaattcaaaa
    36781 tttgcttctt ctttcctccg tggggcggct tctttttcgt atcgaggtat ttatgaaagc
    36841 aataacatta gaacagttcg cagcaatggc gaatgtatcg accagaacgc tgaggcgctg
    36901 gctaaaaaaa ccatcaaccg gagtcccttt gccatttacc ccacgcggta ctcgccaatt
    36961 attctgggag cacgaatgca aagaatggtt gtacagaaca gaacgataat aatcacgcat
    37021 cacattttga ggtaaacatc atgaccaata tcgaaagatt agaacgctgt gccgatatta
    37081 tgcggcgcag atggatttat gaccctgaag cgggtttatt gtttagccgt gaaacaaaag
    37141 acgttatcaa aggcagcatg accaataaag ggcatttggt tgtaacggta cataaaggta
    37201 aattcaaagt gacactcacc tatcagaaag cctgctatat atacacatac ggcgcttatg
    37261 acgagacatt atatgaagtg caccacgtta atttggacaa acaggataac cgcatcaaga
    37321 atattcgcct gatgccgaaa gaaaagcacc gccggataca taaaaacgtc cgcaaactta
    37381 ttcggctggg ccacacacaa tggcttcaac cgcaattaat tgaacaatca ctataaacac
    37441 agtagtaaat accatgcatt cattagacag ttattttcag cgcacgacag cccctaaatc
    37501 agccgcccag gaacgccgtg aggaattcca tgagaaagtg atgcgttctg ctgattatat
    37561 tgcggacaag ttcgtggaaa cggttcgtcc acttgttgat gaagtagctg ataaacttca
    37621 gtctgaaatg ccggaagata tggaaggcac tgcaaagcgg cgactcattt gcgaattaag
    37681 tcgtcgtttt ggcgtgtcaa taagcgcttt taaataatcg aaaaaagggg ggaaagataa
    37741 tggacgcatt tgaggtactg gcaaataaag gcgctgaact ggtagcacag cgtgataaaa
    37801 ccgccaacga aggggaacgc gcactgctaa ataaacagat taaagcgatc cgtatggccc
    37861 agtttaaatt aatcagtaat gaagttatcg aaacaattaa accgcagatc ccacaaatcg
    37921 ttgctgacgc tattaaagcc gccggactgg agaaacgcat tgccgggcta aaaacgaacg
    37981 cagagaaaat ggagatgcac aaacagagcg gtgctccgaa tcccgatgag tacttctcac
    38041 cggatgttga gtttatggcg caggttgagg aacgtctggg tagctgtctc accgaggaac
    38101 agcgtcgcta ttttgatgga gtggatagtt cggcaggtat tgacctgaac tcgtatttcg
    38161 gtcgtgaaat tgagcatttt gatgccgccc cgtctatgcc ggagccagaa ccagaaatgg
    38221 ccaccgatcc tgaagctgag caacggaaac gcgtattcaa tgcgataggg attcggtact
    38281 gatcggataa aagaaagccg ggaattaccc cggctgactt aacaatgtga ccaatgaaca
    38341 aatcggacaa gacaaacagg ataagcagtc tcgaaacaat gttcgggaac tatattagca
    38401 caagtccgga gaaaatcatc atgttggcac tacgcatcat gcagggcatc gctaagaccc
    38461 tcgcagaaca tgtcttagac ctgaaacatt cgccactgag caaacaggcc atgaagcgct
    38521 aaacgcttcg tctgtgggct gaatattcgc ttggaactat caataaaatc atcgacatga
    38581 aatcggggcc gagcaaccag agcgcggagg aaatggagtt catcaggcgt ttaattctca
    38641 ttcgcagaga cattcatagc cagttacaca gcgtgggaat cgatattaat gatgggacgg
    38701 gcgattaatt ttcggacaag tactatgcgc gcgcgaggag ctgaacatga agcaagatat
    38761 tgcagacagg ctggagatac tcgaaggcca gcgggcagaa gcgaaacaac tacgtaagca
    38821 ggccagaaga gcgcacagga acaacgaagc agagttacta accaaataca tcagtttcac
    38881 caattactgc atctacgagt gttacaagga agatgcagag gactggctgg attcattgcc
    38941 agaacagtac tgagcagaat aacaccacgt catgttgttc tgaggtgagc atatcacctc
    39001 tgttcaggtg gccaaattta gtgtgccact tcagcatcac cgttgaaatc acaataacga
    39061 tttttttgta aatatgctct cacatatgta ggaatagacc aggttggcat tgaaacacat
    39121 ctaccatgac cataataaag taattttttg atatgtttat ggttttatat taattaaaat
    39181 atatctaaaa ttgatagttt taaaattgca aaatccagat tgtgtttgta atgtttaatc
    39241 tgattgtatt gagtggcgtt tattatgaaa aataaatcag gtaattcttg ttatagagaa
    39301 gcagtcgata ttcatagctc acttgagctt tcatttatat tgcataaata tcatttgcgt
    39361 gtagatgaga agaaatctcg aaatgtattc agcgccatac gtgatgagct catccttgat
    39421 ggggattcac gacaaaactt agctactttc tgtcaaacat ggcttgatga tgaaatccgt
    39481 gagttgctta atttagctat tgataaaaac atgatttata aagatgaata tccacaaatg
    39541 gcggaacctg aaggtcgctg tgtgcgtatg ctttccgatt cgtggagttt ccccgattcg
    39601 cgacacacct taggttgctc aaccattggt tcatctgaag ctgctatgct cggccgcttg
    39661 gcgttaaaat ggcagtggtg taaaaaacgc gaagtacaag gtaaatcaac cgaacctgat
    39721 ttgcgatcct gtgcaaatat gctggcataa attctgccgt tactgtgatg tgaaattgtg
    39781 tgaaattcca cttgatgatc atcacctgat aatgaatgct aaagaggttt tgaagcatgt
    39841 cgatgaaaac actattggtg tcgttacgac tttaggtatt acttattttt gtaaagcaga
    39901 ttgcgttagt gtttttaaga taagtgttaa agttaatatt agtttttata tttacatcgt
    39961 aattatatgt ttctatttta aaaatattcg agagtataaa aatgtctaat gctaatatcg
    40021 cacccaaaag taagaaaatg acagtgggga cactcgctat catgaatata actgctgtag
    40081 taagtctacg atttatacct tctgaagcag aatacggctt aggtgccatt ttttactata
    40141 cctttgctgc aattatgttt ttagtacctg ttgcctttgt tgcggctgag ctagctacaa
    40201 catatccaca aaaagggggg gcatttcgat ggataggaga agcatttggg gctcgctggg
    40261 gttttgtagg aatgcttatg acctggctac aagtcatccc ctactttcca acagtactga
    40321 catttggtgc agtctcggtt gcatttattg atgttgatat gggggtggca gaatcaattg
    40381 ccagtaataa atggtatatt ttatttttcg tactatttgt ttactgggga gctatttgtg
    40441 ttgcattacg tggcgttggt attttctcgc aggtatctaa gtggtgcggt attataggga
    40501 caattattcc agctattatc cttgttattc ttggtttctc ctatctgtgg ttcagcggta
    40561 aaccagcggt gatcccatta ggttggggtg atcttttccc taatatagct aacttccaga
    40621 atattgttct tgccgccagc atatttcttg cttatgcagg gatggaaatg aatgcggtcc
    40681 atgttaacga tttagataat ccaactaaaa aatacccaat tgcaattacc atcgcttcta
    40741 taggtactat tgcaatattc ctgctcagta ctttaggggt cgcctttatt attcctacag
    40801 ataaaattag tctaactcaa agtttgttgg ttgcctacga tatgctgttc gaatgggctg
    40861 gagtgccatg gcttggctct gtgatggcat ttatgctagc aattggcgta ctgggtgggg
    40921 tggttacttg gattgcaggg cctaatacag gtgtattagc gatcgcgaaa gctggttatt
    40981 tacctaagtt tttccaaaaa accaatcgtc atggaatggg gcaccatcta atgtttgtac
    41041 aaggtataat tgtctcagtt ctttctgtta cttttgtgat aatgccttca gttcaagcag
    41101 cttttcagat tttatcacag ttaacagtta tgctctattt agttatgtat atgctcatgt
    41161 tcgctagtgc tatctatcta cgacattctc aacctgatac aaaacgccca tatcgagccc
    41221 ctgctttatt tttctgggca acacttggat tcttagggtc ttttctggct tttgtattaa
    41281 gttttattcc gcccgagcaa atcccaactg gtagtccaac cagctatgtt ctttatttgt
    41341 tagtattagt tgccctattt ttattaattc caatattaat ttacgccttc cgaaaatctg
    41401 actggaaaga tccaaactct gatttcgaac cattttcttg ggaaaaaaga actagtaatt
    41461 aaattttcca taagctacaa aagatgaggt taaatatgtt tagtgctgaa tctccaattg
    41521 gtgtaataga ctcaggtgta ggtggattga catcggtaaa ggaaataatt aatttgcttc
    41581 ctggtgaaga tattatctat tgtggagata atatgaatgc accttatggc aaccgttctg
    41641 ccgatgatat catcagtttg actaaaaaaa tgctgacttt tttacagtca aggaatgtca
    41701 aactagttgc tgtcgcatgt aatactatat cctcaacttt ggaatccgaa aaatacagcg
    41761 gatatgctaa gagctttccg tttcctatct tatccatcat tgaacctgca gtagaagatg
    41821 tgattcgcca gcaatataaa aatgttggta ttatcgctac cgagtttact attaaaacgg
    41881 ggtgtcacaa agagctaata aaaaaattga attcaacaat aaatgtattt ggagaaccaa
    41941 gcaaaaatct tgcaatgtta attgaggaag gtaatcttaa tgctcctgca atcttgaatg
    42001 acatcaaaaa acatgttaac catttaattt ccctccatcc agtaaatgaa attattctcg
    42061 gctgtactca ctatccaatt gttcagaatt tttttgaagc agttgcaccg gatataaaat
    42121 ttattaatcc tgcacatgat caggctatct ctattaaaaa tcacttggga cagctaaatt
    42181 tattaaactc ctcaaatata ggaactttgc aaataaatac tagtggaagt atggagatct
    42241 acaaaacggt actaggggaa ttgagcatta cgaaacctca tacattcagt atcaggcagt
    42301 tttaaattta tccggtgcac cgtaaaatcc cgtccttctg aactgcaccc caaaagttgg
    42361 acaccaactg agtaaggtgc attttatgga caaacccagt tacatactgg gtagtgcatc
    42421 caattagtag aacatgtgtt tttcgataaa cgctccgatc actttttcgt ggatctcaac
    42481 tgagcgtgag aagcagattg ttttacgacc caaccgctta atgcgggtgc gtagcgtcag
    42541 attattacgc tcaatgcgtt gggtgaatat tttgccggtc agatgcttat tcttcggcac
    42601 ctcccggcca tagctgcccc agtcatcgct ggtcagcatg ccgatgttga agggtgtaag
    42661 cagtgccagt agctcccggc acgtttgatc ggttcgggga ccaaaagtgt aggccagtac
    42721 accgcctgtt ttggtgttgt acgcgtaccc gagccaatgt tgccgggctt tactgccaac
    42781 gtagctccat tgctcatcaa gctcgcagat aaacgccaca tcagcatggg caacaggcga
    42841 agaagttatt cgctttggcg cgagtttttt aaagtccgga tgacggtgtt actaccaatt
    42901 ttcagtgtcc tggcggtatc gcgaaccccg gcaccattaa aggccatttc agttatcagc
    42961 tctttcatac ccggcttacg tgcttgataa gtataagtga actgaaacac acggtggcag
    43021 tcacggcagc gaagtctgtc acggccttta gggttctgac catggcggta aacctgagct
    43081 gactgacaac ggggacaatg aatattaacg ctggtcatga gataacctca aaagcccgta
    43141 ttatacatca gattcaacta attagaggca tcacccatcc agcattataa aacatactaa
    43201 caataactaa attataataa tcacaactta caatcaaata ataaatgcat aagattaaca
    43261 atgatttttt cgggtta
//
LOCUS       DADLXX010000022        40384 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLXX010000022 DADLXX010000000
VERSION     DADLXX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.022"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..789)
                     /locus_tag="JNX27_001160"
     CDS             complement(<1..789)
                     /locus_tag="JNX27_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HAY7882434.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1036..1662
                     /locus_tag="JNX27_001161"
                     /pseudo
     CDS             1036..1662
                     /locus_tag="JNX27_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1659..1865)
                     /locus_tag="JNX27_001162"
     CDS             complement(1659..1865)
                     /locus_tag="JNX27_001162"
                     /inference="COORDINATES: protein motif:HMM:NF019607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7882435.1"
                     /translation="MAAKIIYWALKDKDVIVMSVNGDKVTEDGRILTDRGQRIRDVRT
                     GPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1835..2773)
                     /locus_tag="JNX27_001163"
     CDS             complement(1835..2773)
                     /locus_tag="JNX27_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7882436.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGR"
     gene            complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNX27_001164"
     CDS             complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNX27_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7882437.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3480..4805
                     /gene="rimO"
                     /locus_tag="JNX27_001165"
     CDS             3480..4805
                     /gene="rimO"
                     /locus_tag="JNX27_001165"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7882438.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4852..6180)
                     /locus_tag="JNX27_001166"
     CDS             complement(4852..6180)
                     /locus_tag="JNX27_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7882439.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVESINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6188..8536)
                     /locus_tag="JNX27_001167"
     CDS             complement(6188..8536)
                     /locus_tag="JNX27_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7882440.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFCVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="JNX27_001168"
     CDS             complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="JNX27_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7882441.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="JNX27_001169"
     CDS             complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="JNX27_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7882442.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="JNX27_001170"
     CDS             complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="JNX27_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7882443.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVIPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="JNX27_001171"
     CDS             complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="JNX27_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7882444.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="JNX27_001172"
     CDS             complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="JNX27_001172"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7882445.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15151..16386
                     /gene="moeA"
                     /locus_tag="JNX27_001173"
     CDS             15151..16386
                     /gene="moeA"
                     /locus_tag="JNX27_001173"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7882446.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16386..17135
                     /gene="moeB"
                     /locus_tag="JNX27_001174"
     CDS             16386..17135
                     /gene="moeB"
                     /locus_tag="JNX27_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7882447.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="JNX27_001175"
     CDS             complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="JNX27_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7882448.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18107..19006
                     /locus_tag="JNX27_001176"
     CDS             18107..19006
                     /locus_tag="JNX27_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7882449.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19012..21444
                     /locus_tag="JNX27_001177"
     CDS             19012..21444
                     /locus_tag="JNX27_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7882450.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21590..22405
                     /gene="ybiV"
                     /locus_tag="JNX27_001178"
     CDS             21590..22405
                     /gene="ybiV"
                     /locus_tag="JNX27_001178"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7882451.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22557..23822
                     /locus_tag="JNX27_001179"
     CDS             22557..23822
                     /locus_tag="JNX27_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7882452.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24063..25655)
                     /locus_tag="JNX27_001180"
     CDS             complement(24063..25655)
                     /locus_tag="JNX27_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7882453.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25874..26794
                     /gene="ldtB"
                     /locus_tag="JNX27_001181"
     CDS             25874..26794
                     /gene="ldtB"
                     /locus_tag="JNX27_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7882454.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26852..27970)
                     /locus_tag="JNX27_001182"
     CDS             complement(26852..27970)
                     /locus_tag="JNX27_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7882455.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27967..28434)
                     /locus_tag="JNX27_001183"
     CDS             complement(27967..28434)
                     /locus_tag="JNX27_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY7882456.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28620..28748
                     /gene="mntS"
                     /locus_tag="JNX27_001184"
     CDS             28620..28748
                     /gene="mntS"
                     /locus_tag="JNX27_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7882457.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29020..30603
                     /locus_tag="JNX27_001185"
     CDS             29020..30603
                     /locus_tag="JNX27_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7882458.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="JNX27_001186"
     CDS             complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="JNX27_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7882459.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGLNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31219..31284)
                     /locus_tag="JNX27_001187"
     CDS             complement(31219..31284)
                     /locus_tag="JNX27_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882460.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31519..32406
                     /gene="rhtA"
                     /locus_tag="JNX27_001188"
     CDS             31519..32406
                     /gene="rhtA"
                     /locus_tag="JNX27_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7882461.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32705..33208
                     /gene="dps"
                     /locus_tag="JNX27_001189"
                     /gene_synonym="pexB"
     CDS             32705..33208
                     /gene="dps"
                     /locus_tag="JNX27_001189"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7882462.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33612..34358
                     /gene="glnH"
                     /locus_tag="JNX27_001190"
     CDS             33612..34358
                     /gene="glnH"
                     /locus_tag="JNX27_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7882463.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            34497..35156
                     /gene="glnP"
                     /locus_tag="JNX27_001191"
     CDS             34497..35156
                     /gene="glnP"
                     /locus_tag="JNX27_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7882464.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35153..35875
                     /gene="glnQ"
                     /locus_tag="JNX27_001192"
     CDS             35153..35875
                     /gene="glnQ"
                     /locus_tag="JNX27_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7882465.1"
                     /translation="MIEFKNVSKHFGSTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36136..36330
                     /locus_tag="JNX27_001193"
     CDS             36136..36330
                     /locus_tag="JNX27_001193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882466.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTELAPEPDIEQ
                     KKRPMAHWRMCWITTPRVKS"
     gene            36297..38360
                     /gene="ybiO"
                     /locus_tag="JNX27_001194"
     CDS             36297..38360
                     /gene="ybiO"
                     /locus_tag="JNX27_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7882467.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="JNX27_001195"
     CDS             complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="JNX27_001195"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7882468.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39559..39819
                     /locus_tag="JNX27_001196"
     CDS             39559..39819
                     /locus_tag="JNX27_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7882469.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40084..>40384
                     /locus_tag="JNX27_001197"
     CDS             40084..>40384
                     /locus_tag="JNX27_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7882470.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10048 a        10679 c         9825 g         9832 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ctcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
      841 taagttcggt gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt
      901 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
      961 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagtctgtg gctgcctgtc
     1021 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaactaa cgtaaaaaaa
     1081 gtattgctga cgctcgaata actggaacta ccttatgagc aaattctcgc gggccgtgag
     1141 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
     1201 ttacgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
     1261 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg
     1321 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
     1381 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
     1441 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctct
     1501 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
     1561 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
     1621 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
     1681 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
     1741 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
     1801 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cccaataaat aatttttgct
     1861 gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg ggcgaatctt
     1921 tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtatct
     1981 taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt
     2041 gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac agtgcattac
     2101 tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc
     2161 gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac
     2221 gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc
     2281 ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc
     2341 cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact tttgagagat
     2401 catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg
     2461 aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acgtccagca
     2521 ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca gataatcctt
     2581 ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattat
     2641 cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta
     2701 ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg
     2761 attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga
     2821 taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag
     2881 ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca gcgttaagct
     2941 catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg
     3001 cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact
     3061 ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt
     3121 cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc
     3181 cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag
     3241 atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcaa
     3301 ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct tcacgccgaa
     3361 ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat acaaatttta
     3421 tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta
     3481 tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc
     3541 ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac gtggtaccga
     3601 gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac
     3661 aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg
     3721 gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca
     3781 ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga
     3841 aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg ccgcgtcatt
     3901 atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc attattccgt
     3961 ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc
     4021 tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcctatggcg
     4081 ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc agcatggtca
     4141 gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt
     4201 atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg
     4261 acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg
     4321 tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac
     4381 gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg
     4441 acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag
     4501 gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga
     4561 accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc
     4621 gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg
     4681 cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac attaagccgg
     4741 gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg
     4801 tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg attactgcca
     4861 gctaatctgt gccctgccgt ttttcttact cttatacagc cgctcatccg ccagcatttt
     4921 caagcgttcg acggtagtac atttatttga atgcgctaca ccgatactgg tgcttaaatt
     4981 aatgctttcc acctggaaat gaacgatttc ttttagtatc gatagcaact cttccagcgt
     5041 gccgccagta taaatcaccg caaattcgtc acctccgaag cggtagactt tacctttatc
     5101 taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat cgcccagcaa
     5161 atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacagac acaaataacc
     5221 gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat tttctgccca ttgctttggt
     5281 caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat cggcagccga
     5341 aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg cttctttctc
     5401 cacaatacca cgattaatca ctctgcgtac cagatagagg caactggcac ccgatatcac
     5461 tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc cgcaaataaa catctttttt
     5521 atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg cttccgaaac
     5581 ggtgaagatg gcgtttttag cattatattc atcaccggtc accggtttac cgttcttaaa
     5641 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
     5701 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
     5761 gaaggtttta ccatcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
     5821 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
     5881 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
     5941 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
     6001 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
     6061 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
     6121 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
     6181 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
     6241 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
     6301 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
     6361 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
     6421 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
     6481 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
     6541 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
     6601 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
     6661 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgttga ctaaaatgtc
     6721 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
     6781 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
     6841 ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat atcaacgatc ggttgataga
     6901 cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc cagatattct
     6961 cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac atatggtcac
     7021 ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt tccccctgca
     7081 aaggttcaca aatggcagac ttatggatgc gcagattgcg gtagccaaag ctgctgtcaa
     7141 aatcgtttac catcgctatt ttacgggtaa atgtctgtac attttcccga cacagaagta
     7201 aatagagatc ggcattgaat gtcacgatat cgcagcattg cgcatatttc tcggcaattc
     7261 gctgacacaa tttcctgacg attttattgc gttcatcaac acaaaacacc gcttccacgg
     7321 cttccagacc acacagtttg atgatcccca cagccagata ctgattattg ttggactcaa
     7381 taatcgccat atttttaata aaaccgcttt cctgatcgta agagatcagt gagagcaatt
     7441 tatttgaccg ttcaatttcg gcatctttca tttccgcgat cgaatttttg atgctgataa
     7501 tctctttcgc atcgccttcg cgaattttca gagcctcgat ctgatccgtt gagtcgggca
     7561 aaaacgccag cgtattaacc agctcggtca tttcagaaaa ttgccgacgg aacacacgag
     7621 tggtcatgaa atataccacc agcaacacga taataatgaa cgcaaacggt agataaaaaa
     7681 gtttatctga tgcgttcaat aaattaccgc caggataata aaaatagatt tcgaacaatg
     7741 cattatttgg cactgctgat gtctttgcaa tatactccgt atcgacagaa tcaataatat
     7801 tataattacc ttctctatta taaataatca gtttttcacg agcgagttga ttcttggcaa
     7861 tgatctcatg accttgtttc aaaaatagaa cttcatcatt agcgctgatt tgatcaagat
     7921 actcgttcag ataccgccag tttaaatcga catgaatttt atctaccgct aacaaatcgt
     7981 gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga cgatcaaagc
     8041 aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac cacggtcgtt
     8101 tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc ggtccattta
     8161 cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag agataatatt
     8221 tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc attttcagaa
     8281 tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc tttaatgtca
     8341 gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata agttcaaagc
     8401 ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatc atgaaaaaaa
     8461 gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc tttatctttt
     8521 cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc tgtgccgctg
     8581 caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca aaatcagcag
     8641 aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc tttcaagcaa
     8701 tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca
     8761 acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg
     8821 gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct ggtgtcggcg
     8881 gctgcgcacc taggccgaga aatgacaggc tggcggcaga gataatcgag gtgccaatgc
     8941 gcatggtgaa aaacaccacg atagaagaga cggtcccagg caggatatga cgcaataaaa
     9001 tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca
     9061 ccagcgtgtt gccgcgcacc aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga
     9121 taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac
     9181 ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac cagccttcat
     9241 aatatccagc caacaagccc agcaacgtcc ctatcgccac accgataaac acggcaaaca
     9301 cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca
     9361 gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg tcataatcaa
     9421 aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc acaatcaata
     9481 aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt
     9541 catgccacgg tgtacgtacc tggtcaggtt tgaccagtgg catggcgttt aacaccgcct
     9601 gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt taatggcggc
     9661 gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa gcagaatttc
     9721 cgcctgaatc accgggtaat cgcgcatttc tacagagtca acgagtaagc gtccaagtcc
     9781 gggccagttg aaaactttct ccaccacgat agaacctccg agcaaaaagc caaactgtaa
     9841 gcccatcatg gtcactaccg ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca
     9901 ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta aaacgtcgac
     9961 aaatgacgca cgggtaaagc gcgccatcac ggcggccact gccgcgccga gcgtcaggga
    10021 gggtaaaatg tagtgctgcc aactgtctgc tcccacagta ggcagccagc ccagttcaac
    10081 ggagaatacc tgaattaaaa acatccccag cgcaaatgcc ggaaacgaga tccccgacac
    10141 cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg cgataattcc
    10201 cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc acagcgttgg
    10261 cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg acaggccaaa
    10321 atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg gctgatccaa
    10381 ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgggcc cggcaatcaa
    10441 tcgcgccgga tcgccgggca gcatgtggac aaataaaaac accagcaccg agacgataaa
    10501 cagcgtcgga atcaacccca gtaatcgttt gataacgtaa ttaagcattc cactccctgc
    10561 gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgc tgtctggcat gatccaaaaa
    10621 ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg gatccacggc
    10681 gattcttgcc agatgatatc ctgcgccgcc ttatataagc gggtcttttc cgccggatca
    10741 ttcgttttca gtgcctgagc caggaagtca tccacctgtt tattgctgta aaacgcggta
    10801 ttaaacagcg tcggtggcca gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct
    10861 tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc tttttgccct
    10921 ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg ggctttaatc
    10981 ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta
    11041 tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt cagtaattcg
    11101 cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc gatacttggc
    11161 ggtatcacac cagtagctgg cgttgcatag cccgcaaagg caactttcac cagcgccgga
    11221 cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc
    11281 acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc gatatttttg
    11341 tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg
    11401 gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc
    11461 agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc
    11521 tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc aatctccttg
    11581 ccatattttt ccagcgctgt cggtgaaatc atcgcggtcg ccggatgggc aagaatatta
    11641 ataaacgctg agaacggctg tttgagggta atctttaccg ttgtcggatc gatcgcctcc
    11701 gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg atcgctggcc
    11761 cggtccagat tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt
    11821 ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata actctccgcc
    11881 agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta aaacgatttc
    11941 gctacggcct gagataacgt gtcattcgcg tcatacggat cgagcgtggt gaaattcgat
    12001 cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa cgctgtcgca
    12061 atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt ctccgcctga
    12121 atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat gccccggccc
    12181 gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat tgctgggaag
    12241 atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc
    12301 cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc accgtgaacc
    12361 aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc gctctaccac
    12421 agccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga gatcgagcaa
    12481 caagttgata atctgcccgc gaatagaaac atccagcgcc gaaacggctt cgtcggcaat
    12541 gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc
    12601 ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc gctccagcaa
    12661 ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac gcagcggttc
    12721 gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat
    12781 aaactgaata tcccggcgca atgcctgaag tttgccaggt gacaaggtat cgattcgctg
    12841 accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt
    12901 agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag gccagagatc
    12961 aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca aaccgctgcg
    13021 caaagggaaa cgggtgacaa gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt
    13081 tttctgctcg atggggggtt cctgtttcgc tggatgttca agcgatatca atgggaaacg
    13141 tcggggataa tctaaccctt tcatcgcacc aagttgcgga acagcagcta acagcgcacg
    13201 ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc
    13261 gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca tatcgtgagt
    13321 gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt gcaggatctg
    13381 cgcctgaata gtgacatcca gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg
    13441 gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga gttgatgcga
    13501 ataacgtgaa agaatggttt gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc
    13561 ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt cggcaatctg
    13621 ttcacccaca gtaaatactg ggttcagcga tgtcatcggc tcctgaaata tcatcgccat
    13681 atccgcaccg cgcacatggc gcatttgtgc agcgctctgc tcgctaagtt caatcacatc
    13741 acgactgcgc cgccgcaaca gcattttatc gcactgtact aaaccgcccg cctgttccaa
    13801 cagacgcatc aatgccagcg cagtcactga cttaccggag ccggattcgc ctacaattgc
    13861 cagcgtctca ccgcgttgca gactaaaaga gagattgcgg accgcagcta ttttctgctg
    13921 gtcctgcata aaggcaatat taagattttc aaccgccagc acattaccgg catcaagttc
    13981 atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt ggcgtatcgc
    14041 ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg acattccctt
    14101 catggtcgat agcgattaag ccaccgctac cgccaagcgc agggagtttt tccattacta
    14161 cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg gcgatgtcat
    14221 atgccgccag cgcgcggatg aagacttcgc ctgtgccggt acaagaaacc gccacactgg
    14281 cgttattggc gtagcatccg gcacccacta agggactatc gccaactcgt ccgggtaatt
    14341 tattggtcat tccgcctgtg gacgtggctg ccgccagatt gccgtctaaa tccaacgcca
    14401 cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta tggtcgagga
    14461 ctgttgcccc ttcctcgcgc gctgccagta gttgttcata acgcaaaggc gtggagaaaa
    14521 tctccggtga gacgctctcc atgccatgag caaacgcaaa attttctgcc ccttcgccaa
    14581 tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc ggattacgca
    14641 gatgactaac gcccgccacc gcaccggctt tcagggtgtt accatccatc acacaggcgt
    14701 ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta aacagtggac
    14761 actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg ccggcttcca
    14821 gcattttctg cccggtttca acaatggcag acaacgcctc gatgtagcgt aattcctgtt
    14881 gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca atgactgctt
    14941 tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat atcagaattt
    15001 ttatgaatta ctcattcaat ttttgagtat agaaagatgg atatggcatg taaaggcagg
    15061 agccaacgct acgcccgagg tggcatacag tctatggcgt ttttctgaca taataggcaa
    15121 attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt gatgtcgctc
    15181 gacaccgcgc ttaatgagat gctttctcgc gttaccccac tgaccaccca ggaaacgctg
    15241 ccactggttc agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc
    15301 ccggggtttg ataactccgc tatggacggc tacgcggtgc gtttagccga tattgcctcc
    15361 gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccatacca tggtgaatgg
    15421 cctgcgggta cctgcattcg tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg
    15481 gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg
    15541 cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc
    15601 ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa ttgcttcact ggggattgcc
    15661 gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga tgaactccag
    15721 ttgcccggtc agccgctggg cgacggccaa atctacgaca ccaaccgtct cgccgtacac
    15781 ctgatgttag aacagctggg ttgcgaggta atcaacttag gaattatccg cgacgatcca
    15841 catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt gatcagttcc
    15901 ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga gctgggggag
    15961 atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcattcgg taaactcagc
    16021 aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac cttctatcaa
    16081 ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc
    16141 cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc
    16201 ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg acatcagggt
    16261 tcacatatat ttagctcctt tagtctcggc aactgcttta tcgtgctgga acgcgatcgc
    16321 ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc
    16381 ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa atcattctgc
    16441 gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc
    16501 tcggtggact cggctgcgca gcttcgcagt atctggcaag cgccggtgtc ggcaacctga
    16561 cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagaca ctgcacagtg
    16621 atgccacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg cggatcaacc
    16681 cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt gcagcattga
    16741 ttgctgaaca cgatctggtg ctcgactgta cggataacgt tgcggtacgt aatcaactga
    16801 acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag
    16861 ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt
    16921 tgtttggcga gaacgcgtta acctgcgtag aagcaggcgt gatggcaccg ttgatcggcg
    16981 taattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat ggcaaaccgg
    17041 ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa atgaaactga
    17101 tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc ctgataagac
    17161 gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga acgccttatc
    17221 tgccctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac tccgccacgt
    17281 catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc caaacgctcc
    17341 ctgccagtcc tgctcaaact tcgccacagc ggcttccacc gccggatagc taatcatctg
    17401 ttgtgccaca tccagtggca gagtaattga ttcacatccg gccagtaagc agtccagcgc
    17461 ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat gcattttcaa
    17521 taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat caatacgatt
    17581 aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc
    17641 atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa tcgctgccag
    17701 cccctcggct gtcaccggaa ctttcaccac gatatccgca ataatagaac gcagcttaag
    17761 cgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca gacgcccctg
    17821 accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggttttt tacccgcggc
    17881 gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca gcgccttcac
    17941 cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa tgtacttttt
    18001 gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat caatctaact
    18061 ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga ttttcaatat
    18121 tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc ttaaaggctg
    18181 ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct
    18241 gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg cgccggaagt
    18301 gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc cggagcatct
    18361 gacggcgtta accgactgct gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag
    18421 cgttgaggag atcatggcga ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg
    18481 cggcttaacg ctttcgggtg gtgaaccctt catgcagccg gaaatggcga cggcgctgct
    18541 gcaagcctgc cacgaggcag gcattcatac tgcggtagaa acctgtctac atgtgccgtg
    18601 gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt taaaacacgt
    18661 tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagggtgc tggataacct
    18721 gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga ttcagggctt
    18781 taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg agctgcacgt
    18841 tggcgaaatt cattttctgc cctaccacac gctgggcatc aacaaatatc acttacttaa
    18901 tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gagctgctcg attttgccca
    18961 gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac catgaccaca
    19021 ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt
    19081 gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta tcaacaacat
    19141 ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc
    19201 accatctgga tcaaacacta tgagttgatc attggcaacc aggcaagcga agttcgcgcc
    19261 gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga tgatctggca
    19321 gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg
    19381 tgcccgtggt ggcgcggtca gacagtacag gatcgctgct atggcatgtt taccgatgag
    19441 caaaaaggtc tgctggcgac cggcatcatt aaagcggaag gcaatatgac ctccggcgat
    19501 gcgcacctgg cggtcaattt cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag
    19561 aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa
    19621 cagttcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat tgaacgtttc
    19681 gctgccctgg cgcgtgcaat ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg
    19741 ctggcgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac tttctggcag
    19801 gcgctgcaac tgtgttactt catccagttg attttgcaga ttgaatctaa cggtcactcg
    19861 gtgtcgtttg gtcgtatgga tcagtatctc tacccgtact atcgccgcga cgttgaactg
    19921 aaccagacgc tggatcgcga acacgccatc gaattgctgc atagctgctg gttgaaattg
    19981 ctggaagtga acaaaatccg ctccggctcc cactcaaaag cctctgcggg aagtccgctg
    20041 tatcagaacg tcaccattgg cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg
    20101 aatccactct cttacgcgat cctcgaatcc tgcggtcgcc tacgttccac tcagcctaac
    20161 ctcagcgtgc gttaccatgc aggaatgagt aacgatttcc tcgacgcctg cgtacaggtg
    20221 atccgctgcg gcttcgggat gccggcattc aacaacgacg aaatcgtgat cccggaattt
    20281 attaaactcg gtattgaacc gcaggacgct tatgactacg cagcgattgg ttgtatagaa
    20341 accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat caacttcgcc
    20401 cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg
    20461 ccacaagaaa aagcgttgtc ggcaggtaac ttcaacaact tcgatgaagt gatggccgcg
    20521 tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac
    20581 accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga tgactgtatt
    20641 gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc tggcctgcag
    20701 gtcggtattg ccaacctcgg caacagcctg gcggcagtga agaaactggt gtttgaacag
    20761 ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga tggcctgact
    20821 catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa cgacgatgat
    20881 accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact gaaacagtac
    20941 cataacccac gctacggtcg tggtccggtt ggcggcaact attacgcggg tacgtcgtct
    21001 atctccgcta acgtaccgtt tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc
    21061 cacaccccgc tggcagaagg cgctagtccg gcctctggta ctgaccatct cggccctact
    21121 gcggtcattg gctcagtggg taaactgcct acggcagcga ttctcggcgg cgtgttgctc
    21181 aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa actgatgatc
    21241 ctgctgcgta ccttctttga agtgcataaa ggctggcata ttcagtacaa catcgtttcc
    21301 cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt
    21361 gtcgcgggtt attccgcctt cttcaccgcg ctctctccag acgctcagga cgatatcatc
    21421 gcccgtactg aacatatgct gtaatctctt cggcccggct gcggcgtcgc cgggctaaat
    21481 cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg ctctgcgtca
    21541 cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa
    21601 agttatcgtc acagatatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc
    21661 acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg ttgtcgccag
    21721 cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt
    21781 tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact
    21841 gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt
    21901 tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc
    21961 actgatggca aaacactacc atcgcctgaa acctgtaaaa gattatcagg agattgacga
    22021 cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa
    22081 actacacata gcgctggatg gcattatgaa acccgtcacc agtggttttg gctttatcga
    22141 cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga
    22201 tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa
    22261 aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg
    22321 ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga
    22381 taacacatcc ccttttaaca gctgacctct ctaccggagc catgcgctcc ggttttccct
    22441 tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact
    22501 tgtattgtca catgcaaatc agcatagttt cgaatgaaag ttaatgcgag gtggttatgg
    22561 cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga
    22621 agcacgcgct gcgggcgcag cttggcgatg tccagcagat ctttaatcag ctaagcgatg
    22681 acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc
    22741 cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga
    22801 ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct
    22861 gggatcagtc gatgctggac tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc
    22921 ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact
    22981 ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc
    23041 tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg
    23101 tcatctaccc tgaccgtatc cgccgccttc cgcccggaac gacctccaaa ggtcttggag
    23161 cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag cacgttttcg
    23221 ccaacgtctt taatggcaat ctggcgaaat acgatccctg gcatgcggca catcgtacgg
    23281 aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccagg
    23341 gctggactgc gctctcggat atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc
    23401 ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac
    23461 tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat ccactgttaa
    23521 ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg tggcactgcg
    23581 acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca
    23641 ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac
    23701 tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag
    23761 gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttgggc atggatgttt
    23821 gagagttaat ggaagtgaat tgatgtggga ttaagaaaat cgattcgacg ttactcagat
    23881 tgctgacaag tgcgctttct taatgcctga tgcggcgtta acgccttatc cggcctacaa
    23941 agttctgcag attcaatata ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag
    24001 gctattttgc atttgtcatc agccgtcgtc accccgacag tatcggggtg acggataatc
    24061 acttactcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg
    24121 cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc acggtcgtgt
    24181 gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga ctcgatggat
    24241 tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt
    24301 aacttaccaa acagcatccg ccctttttcc ccaccggaaa gcactttagc tggctttttg
    24361 atgtcgtcct ggctgaacag caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg
    24421 ccttcctgct tccactgact catccattcg aacacggtca gatcattttc aaactcatat
    24481 tcgtggtcct gggcgtagta accaatgcgc gcgttttcag accattttac ggtgccgctg
    24541 tccggttgca gatcgcccac cagcgttttc agcagcgttg atttaccgac gccgttggta
    24601 cccagtaccg caagtttttc accgacctcc agcagcaggt tgagattttt aaacagtgga
    24661 ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa cagtttctta
    24721 tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttcacctc ttccagtttg
    24781 attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta
    24841 aagcggctaa cgaaagattg caactcagca atctgcgctt tcttcttggc gttatcggcc
    24901 agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt acccggataa
    24961 acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt aaggaagtga
    25021 cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg ttccagccag
    25081 cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat atccggctca
    25141 gcaaacagcg cctgcgccag aagcacacgc agcttccagc caggagcaac ttcactcatc
    25201 gggccgtagt gctgttccac tggaattccc acgccaagca acagttcacc ggcgcgagct
    25261 tccgcagagt aaccgtccat ttcgccgtat ttcacttcca gatcggccac tttatagccg
    25321 tcttcttcac tcatttccgg caaagcatag atgcggtcgc gctcctgctt cacttcccac
    25381 aactctttat gccccatgat caccgtatcc agcacagtga acccttcaaa ggcaaactga
    25441 tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc
    25501 tcaaggtcgc cgccgagaat cttcataaag gtggatttac cactaccgtt cgcgccaatc
    25561 aggccgtaac ggttgccgcc gccgaatttg acggaaatgt tttcaaacaa cggcttactg
    25621 ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa agagatatga
    25681 caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca
    25741 ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt gagctggaat
    25801 gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg ccaggcacaa
    25861 aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct tcgctgttgt
    25921 cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct
    25981 ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc tggagtattt
    26041 tgccgcggag taccagatgg gactttccaa tatgatggaa gcgaacccgg gtgtggatac
    26101 cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc tgccggatac
    26161 cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact atccgaaagg
    26221 gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag atacgcctat
    26281 caactggacc accaaagttg agcataagaa agcaggcccg acctggacgc cgaccgccaa
    26341 aatgcacgca gagtaccgcg ctgcgggcga accgctaccg gctgtcgttc ctgcaggtcc
    26401 ggataacccg atgggtctgt atgcactcta catcggtcgc ctgtatgcta tccatggcac
    26461 caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt tgtgtgcgtc tacgtaacga
    26521 agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt ttattgatga
    26581 gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc ataacccgct
    26641 gtctaccacc gaagcccagt ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag
    26701 cgtgcagaca gtgaccggtc agccagatgt tgaccaggtt gttcttgatg aagcgatcaa
    26761 aaatcgttcc gggatgccgg ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga
    26821 ctcataatcc gcctttttta tttgccagac cttaccaggc cgggatcata actaacaaaa
    26881 cgtatcccac cagcgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc
    26941 agatgcggcg atcgttcgcc atacgtagcg caatcaaatt tgccagcgat cctggtaata
    27001 aaccaaagcc ccccacgttt accgcccatg ccagtaataa agacggaggt acataattga
    27061 gcagcaaaat ggtactcggc acgttactga tcacctgcga taaaccgact gccgttaacc
    27121 ataacccggg ttcagataaa tgactcacgt tacccaacac gccttgcaac gctggaagct
    27181 gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcaac gtccagtcca
    27241 cactcaacac cacgcgacgc gccagcagca caaagcccgc cgccacaatc accagtcccc
    27301 acagctcttg tttgaactcc agcgccgtca gaaaaacgat atacagcccc agacaattcc
    27361 acaccagccg cggtttccac tccggtgttt gcatccccgt atggtattgc agcgcctttc
    27421 cagggaaaca acaccagcac aacagcagga gtgtcagcat cattgcgcca gccagcggtg
    27481 ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt
    27541 gcgggttgcc aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa
    27601 tcagccgatt aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa
    27661 taaacagcgc gacatcgttg gtctgaaagg tagaaagcag cgccgccgcc agcaccataa
    27721 acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa
    27781 aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg
    27841 tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc
    27901 cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag
    27961 gcaggctcat ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc
    28021 actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa
    28081 caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc
    28141 tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc
    28201 cagccgctta agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc
    28261 cacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc
    28321 aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac
    28381 gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct
    28441 tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag
    28501 ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata
    28561 tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta
    28621 tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa
    28681 tgctgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttcag
    28741 ataaatagcg gcgtcgcggt acgccgcttc actcctgctt ttatgcaggc ataacgcgtt
    28801 ttggtctgaa aaaaaccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc
    28861 gtcagtagtt gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg
    28921 gctcgcggat gcggacccct ttcccctctt cacgcactct tgcaggtatt gacccttgac
    28981 gccagggtaa gcacatggcg tttgttacca tagtggcata tgaatttaac cctcaaagaa
    29041 tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg
    29101 ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg
    29161 gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc
    29221 agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc
    29281 aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt
    29341 tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc
    29401 aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta
    29461 ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt
    29521 aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat
    29581 aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg
    29641 acggtcactg ccgttaagcc gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg
    29701 cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc
    29761 gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt
    29821 ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc
    29881 ctagcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa
    29941 tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa
    30001 ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa
    30061 gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat
    30121 ccgcaggcct gcgacaggac acaaggcaaa tacaaaacct ttgtgcaatc gaaagaaacg
    30181 tcgtgctatc tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag
    30241 ttacgcaaca gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt
    30301 aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc
    30361 caggtgcctt ttatggtcat ttccagcggc gataaagcgc atcgggtgat taaagcccgc
    30421 cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa
    30481 ataaatatca aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac
    30541 ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct
    30601 taaaaacaaa aatccgcccc gagaggcgga tttttatatc accaaagtga ttagaagcgg
    30661 taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa
    30721 gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa
    30781 ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa
    30841 cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca
    30901 gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg gcttttctcg
    30961 gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc
    31021 aggtttaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca
    31081 gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt
    31141 gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag
    31201 ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca
    31261 tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca
    31321 gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca
    31381 ccagactaat gattccatca atcctggcgc attttagtca aaacggggga aaattttttc
    31441 aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag
    31501 atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata
    31561 gtcatattac tcgttgccat ggcgtctatt cagggtggag cctcgttagc taagtcactt
    31621 tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg aacgctgatc
    31681 ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg
    31741 ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc tattcagaca
    31801 gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc
    31861 tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc
    31921 ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggcactg
    31981 ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcggaacat
    32041 ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga
    32101 gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg tctggctgtc
    32161 gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca
    32221 acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg
    32281 attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc tatcatcgcc
    32341 gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga attagacatt
    32401 aattaaattt acatttctgt atggttatgc ataaccatgc agaatttctc gctacttttc
    32461 ctctacaccg tctttatata tcgaattatg caaaagcata tttattccga aaattcctag
    32521 cgagcagata aataagaatt gttcttatca atatatctaa ctcattgaat ctttattagt
    32581 tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca
    32641 catagccggt gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga
    32701 aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa
    32761 cgatgtctcc gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca
    32821 gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt
    32881 cattgccgta catgaaatgc tggatggctt ccgcaccgca ctgatcgatc atctggatac
    32941 catggcagaa cgtgccgtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa
    33001 cagcaaaacc ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa
    33061 agaactggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc
    33121 gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct
    33181 gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtacc
    33241 cgataaaagc ggcttcctga caggaggccg ttttgttttg cagcccacct caacgcactt
    33301 atttagtgca tccatctgct atccccagct gattaagtaa attttttgta tccacatcat
    33361 cacacaaccg ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg
    33421 ttgagtgcac aattttagcg caccagattg gtgccccaga atggtgcatc ttcagggtat
    33481 tgctctataa atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa
    33541 ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa
    33601 aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg
    33661 cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc
    33721 cgtttgaatt taaacagggc gataaatatg tgggctttga cgttgatctg tgggctgcca
    33781 tcgctaaaga gctgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc
    33841 cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc
    33901 gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga
    33961 aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga
    34021 agagcggtac tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc
    34081 agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc
    34141 tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag
    34201 cggtaggtga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg
    34261 agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg
    34321 aagtctacaa aaaatggttc agtactgaac cgaaataata acgctacacc tgtaaagcgc
    34381 actggcagtt ccctctcccc cctggggaga ggattagggt gaggggcgca aacccgctcc
    34441 ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc
    34501 agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga
    34561 ccctgtggat ttcggtcctc ggtctggctg gcggtctggt aatcggattg ctggcaggtt
    34621 ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga
    34681 tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt
    34741 ttaacgactt acgcatcgac ccatttactg cggcggtggt caccatcatg atcaactccg
    34801 gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg
    34861 aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc
    34921 tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca
    34981 cctcactgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg
    35041 ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta
    35101 ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga
    35161 atttaaaaac gtctccaagc actttggctc aacccaggtg ctgcacaata tcgatttgaa
    35221 cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct
    35281 gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct
    35341 gaaggttaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag gtatggtgtt
    35401 ccagcagttt tacctcttcc cgcatctgac ggcgctggga aacgtcatgt ttggtccgct
    35461 acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa
    35521 agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca
    35581 gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc
    35641 gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc
    35701 tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc
    35761 ttcgcggctg atctttatcg acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt
    35821 gatcaagaac ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaataaga
    35881 cacattgcct gatgcgctac gcttatcagg cctacaggat atctggcaac ttattaaaat
    35941 tgcatgaact tgtaggacgg ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg
    36001 ttgtcagcaa ccggcttaat atcccttcct ccctttcacc cgaaagggag gcacaccaga
    36061 ttcctctcat ttaaaatcgc ccctccccca gcatctatac ttatcttttt gctctgtttt
    36121 ctcactggag gagtcatgcg gtggatcctg ttcatcctct tctgcctgct gggcgcacct
    36181 gcccacgcgg tatccatacc cggcgttaca accacaacga caacggactc aacgactgaa
    36241 ctggccccgg aaccggatat cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc
    36301 tggataacga cacctcgcgt aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc
    36361 cccctgcgga accggtcccg aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc
    36421 tgcaaaaggt caccgaagtc agccgccatt atggtgaagc cctttccgcc cgcttcgggc
    36481 aactttatcg caatatcacc ggctccccgc ataagccatt taatccacaa accttcagca
    36541 atgcgctgag ccatttttca atgttagcgg tattagtgtt tggtttttac tggctgattc
    36601 gcctgtgcgc actgccgcta tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg
    36661 agcgcagcaa ctggttgcag cttccggcga tgattattgg ggcgtttatt atcgacctgc
    36721 tgttactggc cctgacatta tttgtcggcc aggtattaag cgacaacctg aatgcaggta
    36781 gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg attgaatttt
    36841 tcaaagccgt actacgcctg attttttgcc caaacgtggc ggagctgcgc ccgttcacga
    36901 ttcaggacga gagcgcccgt tactggagtc gccgcctgag ctggttaagc agcctgattg
    36961 gctatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta cagataggtg
    37021 cgctggcgaa cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc
    37081 gaaataaaaa agagattacc cagcatttgc tcaactttgc ggagcattcg ctggcttttt
    37141 tcagcctgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc gcctatttta
    37201 tcgtgctgtt tttcttttcg ttgttcgatc cgggcaacag tctgaaattt atgatgggtg
    37261 caacggtgcg cagcctggcg attattggta tcgcagcgtt tgtttccggt atgttttccc
    37321 gctggctggc gaaaaccatc accctctcgc cacatactca gcgtaactat ccggagctac
    37381 aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct
    37441 gcgtggcagt aatgttgctg ttgagcgcat ggggattgtt cgatttctgg aactggctgc
    37501 aaaacggcgc ggggcagaaa accgtagata tcctgatccg catcgcactc attcttttct
    37561 tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg gcttcggata
    37621 ttcatggccg cccgctaccc agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg
    37681 cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa atcggcgtca
    37741 atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctcg tttggttcgc
    37801 aaacgctggt aaaagatatt atcaccgggg tatttattca gtttgaaaac ggcatgaaca
    37861 ctggagattt ggtgactatc gggccgttga ccggcacagt ggaacggatg tcgattcgct
    37921 ccgtgggcgt gcgccaggat accggggcgt atcacatcat tccgtggtct tcgataacca
    37981 cctttgctaa cttcgtccgc ggtattggtt cggtagtggc aaactacgat gttgatcgcc
    38041 atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa
    38101 acgaagaaat tcgcgggctg attattggtg aaccgaactt tgccgggatt gtcggcttaa
    38161 gcaataccgc gtttacactg cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg
    38221 tacgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc
    38281 cagtgcagac ttatcaggtg ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc
    38341 cgggggagcc aacgctttaa cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca
    38401 taaaggtcca ggcaataaag cgactctgct tttgcccctg ggccatctct ttttttacca
    38461 ccttcaccgc gcccacgtcg gtcagagcac gatacaacgg cggtaagttt tcaccacgag
    38521 ataccagcga ggtaaaccac atcacctgct tcgcgaagcc tttgctctct tcaatcatct
    38581 ttttgataaa gttgacttca ccgccttcac accacaactc ctgttgctga ccaccaaagt
    38641 tcagtgcatc gtctttgttc agccccaggt tacggcgttt acgctcacta cctgcccgcg
    38701 ccgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt
    38761 tgtggatgat gccgttaaaa atcgccccac tctctttttg ccgacgcaga cgaatggcgc
    38821 ggttaagccc cggattagca ctgataatcg cctgcgcact gcttaacgcc tggctgctgg
    38881 tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga taaatacagt
    38941 tcgcaccaac gccgatatcc agaatgctgg cattcgctgg aattgttccg cttgcttcag
    39001 ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggt gggcagagaa
    39061 aaccgtcggg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac gccttattga
    39121 gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg
    39181 tgaggaattg cctgagttca ggattgacct gacaaagcgt ggcgagatcg tagcggctgt
    39241 gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc tcctttaagc
    39301 agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt ttatcattcc
    39361 ccaaaacctt aacatggcta tggtttatgg caattcatca taaaaatcag cattatgcat
    39421 gtcattgatt tgtattattt tgtaaaaata tatcgttcaa aaaataagca tatctctgtc
    39481 aattttttgc gtcatttgca tcgcgcaggc gacctacact tatcaggcac tacccgttgt
    39541 atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac cgtcctgagc
    39601 ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag cggtcagcta
    39661 cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct ggaggataaa
    39721 ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg tcattaattc cgcaggcgga
    39781 aatgaccaga tgttcggtac tgcaaccatc tacaaataac cccaccaacg taactgcatt
    39841 cgcctctgga tgcagttttc gcctgttttg taatcaaaaa acatcaaata tgaacgcaat
    39901 gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcgtc aagtgataat
    39961 acttatcaaa attattatca cttgcacaaa cactatcacg ggattaacag tggcatcgca
    40021 tccgcagaga tgctttctcg tggcagtgaa aatttcaaca tataagaaaa agtcacctgc
    40081 aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac gttctttgcc
    40141 ggtctttgta ttggtattac gcctgtggca caggcactcg ccgccgaagg gcaagctaac
    40201 gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc acaacaatct
    40261 gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac ggtgatttct
    40321 gaacaagtga ttaaagatca gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc
    40381 ggcg
//
LOCUS       DADLXX010000023        39556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLXX010000023 DADLXX010000000
VERSION     DADLXX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.023"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..188)
                     /locus_tag="JNX27_001198"
     CDS             complement(<1..188)
                     /locus_tag="JNX27_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY7882471.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="JNX27_001199"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="JNX27_001199"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7882472.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="JNX27_001200"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="JNX27_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7882473.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="JNX27_001201"
     CDS             3865..4146
                     /locus_tag="JNX27_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY7882474.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4439..4981
                     /locus_tag="JNX27_001202"
     CDS             4439..4981
                     /locus_tag="JNX27_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7882475.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5062..5736
                     /locus_tag="JNX27_001203"
     CDS             5062..5736
                     /locus_tag="JNX27_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7882476.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5752..8232
                     /locus_tag="JNX27_001204"
     CDS             5752..8232
                     /locus_tag="JNX27_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7882477.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPLVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8248..9281
                     /locus_tag="JNX27_001205"
                     /pseudo
     CDS             8248..9281
                     /locus_tag="JNX27_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9363..9701)
                     /gene="rcnB"
                     /locus_tag="JNX27_001206"
     CDS             complement(9363..9701)
                     /gene="rcnB"
                     /locus_tag="JNX27_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY7882478.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9920..10768)
                     /locus_tag="JNX27_001207"
     CDS             complement(9920..10768)
                     /locus_tag="JNX27_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY7882479.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            10889..11161
                     /gene="rcnR"
                     /locus_tag="JNX27_001208"
     CDS             10889..11161
                     /gene="rcnR"
                     /locus_tag="JNX27_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY7882480.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11253..11349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11384..12172
                     /gene="thiM"
                     /locus_tag="JNX27_001209"
     CDS             11384..12172
                     /gene="thiM"
                     /locus_tag="JNX27_001209"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY7882481.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12169..12969
                     /gene="thiD"
                     /locus_tag="JNX27_001210"
     CDS             12169..12969
                     /gene="thiD"
                     /locus_tag="JNX27_001210"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7882482.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13034..13852
                     /locus_tag="JNX27_001211"
     CDS             13034..13852
                     /locus_tag="JNX27_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY7882483.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13904..14650
                     /locus_tag="JNX27_001212"
     CDS             13904..14650
                     /locus_tag="JNX27_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7882484.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14624..15589)
                     /locus_tag="JNX27_001213"
     CDS             complement(14624..15589)
                     /locus_tag="JNX27_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7882485.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15586..16589)
                     /locus_tag="JNX27_001214"
                     /pseudo
     CDS             complement(15586..16589)
                     /locus_tag="JNX27_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16586..17062)
                     /locus_tag="JNX27_001215"
     CDS             complement(16586..17062)
                     /locus_tag="JNX27_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7882486.1"
                     /translation="MLARSSFHRKGIQKVLLCGIVIAYLTLPVNFTYALLFLGILLHG
                     VSYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQE
                     PVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17216..18268
                     /gene="fbaB"
                     /locus_tag="JNX27_001216"
     CDS             17216..18268
                     /gene="fbaB"
                     /locus_tag="JNX27_001216"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7882487.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18576..19430
                     /gene="gatY"
                     /locus_tag="JNX27_001217"
     CDS             18576..19430
                     /gene="gatY"
                     /locus_tag="JNX27_001217"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY7882488.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRLKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNLAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19459..20721
                     /gene="gatZ"
                     /locus_tag="JNX27_001218"
     CDS             19459..20721
                     /gene="gatZ"
                     /locus_tag="JNX27_001218"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY7882489.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAVVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            20731..21183
                     /gene="gatA"
                     /locus_tag="JNX27_001219"
     CDS             20731..21183
                     /gene="gatA"
                     /locus_tag="JNX27_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7882490.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21214..21498
                     /gene="gatB"
                     /locus_tag="JNX27_001220"
     CDS             21214..21498
                     /gene="gatB"
                     /locus_tag="JNX27_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7882491.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQYRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21502..22858
                     /locus_tag="JNX27_001221"
                     /pseudo
     CDS             21502..22858
                     /locus_tag="JNX27_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            22906..23946
                     /gene="gatD"
                     /locus_tag="JNX27_001222"
     CDS             22906..23946
                     /gene="gatD"
                     /locus_tag="JNX27_001222"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7882492.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24046..24825
                     /locus_tag="JNX27_001223"
     CDS             24046..24825
                     /locus_tag="JNX27_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7882493.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQEAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(24907..25806)
                     /gene="yegS"
                     /locus_tag="JNX27_001224"
     CDS             complement(24907..25806)
                     /gene="yegS"
                     /locus_tag="JNX27_001224"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY7882494.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            26212..26529
                     /locus_tag="JNX27_001225"
     CDS             26212..26529
                     /locus_tag="JNX27_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882495.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(26871..28232)
                     /locus_tag="JNX27_001226"
     CDS             complement(26871..28232)
                     /locus_tag="JNX27_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7882496.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28379..28701)
                     /locus_tag="JNX27_001227"
                     /pseudo
     CDS             complement(28379..28701)
                     /locus_tag="JNX27_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(28892..29614)
                     /gene="baeR"
                     /locus_tag="JNX27_001228"
     CDS             complement(28892..29614)
                     /gene="baeR"
                     /locus_tag="JNX27_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7882497.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHESGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(29611..31014)
                     /gene="baeS"
                     /locus_tag="JNX27_001229"
     CDS             complement(29611..31014)
                     /gene="baeS"
                     /locus_tag="JNX27_001229"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7882498.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31011..32426)
                     /locus_tag="JNX27_001230"
     CDS             complement(31011..32426)
                     /locus_tag="JNX27_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7882499.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(32427..35492)
                     /gene="mdtC"
                     /locus_tag="JNX27_001231"
                     /pseudo
     CDS             complement(32427..35492)
                     /gene="mdtC"
                     /locus_tag="JNX27_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
     gene            complement(35493..38615)
                     /gene="mdtB"
                     /locus_tag="JNX27_001232"
     CDS             complement(35493..38615)
                     /gene="mdtB"
                     /locus_tag="JNX27_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY7882500.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPSASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(38615..>39556)
                     /locus_tag="JNX27_001233"
     CDS             complement(38615..>39556)
                     /locus_tag="JNX27_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HAY7882501.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         9918 a         9685 c        10238 g         9715 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcattcctg taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tatttatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccta ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaaatcatt tatataaatg
     4201 atttcgtctt ttttattgat atcaacaata ccatttacat aaatctacat taattaatag
     4261 atgtgataaa tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt
     4321 gttttaaatc aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt
     4381 gtttccaaga gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat
     4441 gaaacgttca attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt
     4501 tgctgcagac gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg
     4561 taaattcgaa gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt
     4621 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat
     4681 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
     4741 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtgg gttactatct
     4801 ctatttaggt gataacagcg atgtcctaga tgtttctgca ccttttaaca ttgagagtta
     4861 taaaacagca gaaggtcaat atgctattcc gtttaaagca aaatacctga aactgacaga
     4921 taactcagtg caatcaggtg atgtgttatc ttctctggtt atgcgtgtag cgcaggatta
     4981 ataaataaaa ttgcaagttt ctccaggtaa taaacatggc cgctatcccc atggcggctt
     5041 tttaatttaa gaggcattaa gatgaaagga ttattatctt tactcatttt ttctatggtc
     5101 cttcctgcac atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat
     5161 aaagaagtta tggtgcagtt gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg
     5221 attgatgatg gcgatacgtc gttaccacca gaaaaaattc aggttccttt catgttaacg
     5281 ccaccagtgg caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg
     5341 aataaactgc ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca
     5401 aatagtccag agcaagaagg caagaatgca ctgaagtttg cgatgcaaaa cagaattaag
     5461 ttgttttacc ggccagcggg tattgctccg gtaaataaag cgacctttaa aaagttactg
     5521 gtaaatcgca gtggcaatgg attggtgata aaaaatgact cagctaattg ggtgacgatt
     5581 tcggatgtca aagctaataa tgttaaagtc aattatgaaa ctattatgat tgccccctta
     5641 gaaagtcaga gtgttaatat caaaagtaat aatgcaaata actggtatct gaccattatc
     5701 aatgaccacg gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga
     5761 atgaccccac ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct
     5821 gaagaaacct ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat
     5881 attcgtcttg atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat
     5941 aagcaatggc gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta
     6001 tcaagagaag ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa
     6061 tgtttaacat ttgagcaact tgttcagggt gggagctata gctgggatat cggggttttt
     6121 cgtctcgatt tcagtgtccc gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca
     6181 ccggaaaact gggagcgggg tattaatgcg ttttatactt cttattatgt gagtcagtat
     6241 tacagcgact ataaagcgtc gggtaacaac aagagtacat atgtacgttt taacagcggg
     6301 ttaaatttac tggagtggca actgcattct gatgccagtt tcagtaaaac aaataacaat
     6361 ccaggggtgt ggaaaagcaa taccctgtat ctggaacgtg gatttgccca atttctcggc
     6421 acgcttcgcg tgggtgatat gtacacatca agcgatattt ttgattctgt tcgcttcagc
     6481 ggtgtgcggt tgtttcgtga tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca
     6541 cgggtgcagg ggattgctca gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg
     6601 gtttatcaga aagaggttcc tcctggcccg tttgcgatta cagatttgca gttggccggt
     6661 ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg gctcggtaac cacctatctg
     6721 gtgccttatg cagcggtgcc aaatatgctg caacccggcg tgtcgaaata tgattttgcg
     6781 gcgggccgta gccatattga aggggcgagc aagcaaagtg attttgtcca ggcaggttat
     6841 cagtatggtt ttaataactt attgacgctg tatggcggca cgatggttgc taataattac
     6901 tatgcgttta ccctcggaac gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc
     6961 acgaagtcgc atagtaaaca agacaacggc gatgtgtttg acgggcaaag ttatcaaatt
     7021 gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat
     7081 tcgtcgcgtg attaccggac atttaacgat cacgtttggg caaacaataa agataattat
     7141 cgccgtgatg aaaacgatat ctatgacatt gccgattatt accagaacga ttttggccgt
     7201 aaaaatagct tttctgccaa tatgagccag tcattgccag aaggctgggg ttctgtgtcc
     7261 ttaagtacgt tatggcgaga ttactggggg cgtagcggca gcagtaagga ttatcagttg
     7321 agttattcca ataactggcg gcggataagc tataccctcg cggcaagcca ggcgtatggc
     7381 gagaatcatc atgaagagaa acgttttaat atttttatat caattccctg tgattggggt
     7441 gatgacgtta cgacgcctcg tcggcaaata tatatgtcta actcaacgac gtttgatgat
     7501 cagggttttg cctcaaataa tacgggatta tcaggaacag tagggagtcg ggatcagttc
     7561 aattatggtg tcaacctgag tcatcaacat cagggaaatg aaacgacagc tggggcgaat
     7621 ttgacctgga acgcgccggt tgcgacagtg aatggcagtt atagtcagtc gagtacttat
     7681 cgacagactg gagccagtgt ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg
     7741 gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc caggaattaa agatgcttat
     7801 gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag tggtggtata cgacggaatg
     7861 acaccttatc gggaaaatca cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa
     7921 ttacgtggca accggaaaat tgccgcccct tatcgcggcg cggttgtact ggttaatttt
     7981 gataccgatc agcgcaagcc ctggtttata aaagcgttaa gagcggatgg tcaaccatta
     8041 acgtttggtt atgaagtcaa tgatatccat ggtcataata ttggcgttgt cggccaggga
     8101 agccagttat ttattcgcac caatgaaata ccgccattgg ttaatgtagc aattgataag
     8161 caacaaggac tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag tagaaattat
     8221 atttgccagt aatagtcagg tggttctatg gaaattcgca taatgctatt tatattaatg
     8281 atgatggtta tgcctgtgag ctatgcggca tgttatagtg agttatctgt tcagcacaat
     8341 ttggttgttc aggggaattt tgcacttact caaacacaaa tagcgacata tgagcataat
     8401 tttaatgatt cgtcatgcgt gagtacaaat actatcaccc ctatgagccc atcggatatt
     8461 attgttggac tttataacga taccattaaa ttaaatttac attttgaatg gaccaataaa
     8521 aacaacatca cgttgtcaaa taatcagagc agtttcacca gtggttattc ggtaacggtg
     8581 acacctgcgg ccagtaatgc aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg
     8641 attaatggtg ttgcgacatt atccagtgct tcatcatcga cacgcgggag tgccgcagta
     8701 caatttctac tgtgtttatt aggtggcaag tcatgggatg catgtgtaaa tagctacaga
     8761 aatgcattgg cacaaaatgc aggtgtctat tccttcaatc tgacattgtc atacaacccg
     8821 ataaccacaa cctgcaaaca ggacgattta ttaattactt tagacagtat tcccgtttca
     8881 caattaccag ccacaggtaa caaagcaaca ataaatagta aaaaaaggga tattattctg
     8941 cgttgtaaaa atttattagg tcaacaaaat caaacatcac ggaaaatgca ggtgtattta
     9001 tcaagttctg acttgttaac caacagcgac acaatactga aaggtgcgga agataatgcg
     9061 taggatttat tcttgaaagt aatggttcgc cagtcacact tttaaatatc actaacagca
     9121 gtaaaggata cacaaattta aaagaaattg cggcaaagtc aaaacttaca gatacaacgg
     9181 tttcaattcc gataacagcc agttactacg tctacgatac aaacaaagtt aaatctggcg
     9241 cactggaggc aaccgcatta atcaacgtga aatacgacta ataacaagca agacgagcaa
     9301 gtggctaata ataaaaaata acaaggtgtc tgcatttccc cctcatgatg agggggcttt
     9361 ttttagcgat gataaaaaat ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg
     9421 ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg tcccggcatc aggcgcgggc
     9481 aggttacgta actgccactg cttaatgttg tactcatcgg tgcggtacat ctctgggacg
     9541 gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac tacttaactc atacttagaa
     9601 attcccgagg tattggtcga acccgcggtg gctggtgcgg cccaggcggc actggtaacc
     9661 agcaaaagca cacccagcaa catcttattt ttaatggtca taaactctcc actttgggga
     9721 tctgtaatat cagtcgcata tctttcctgc catacaatat aactgacaag cgatattttg
     9781 ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg atgccctaaa agcatcctta
     9841 gcgagcagag agtcagcgga aatagctgat ttaagaatca tcctgggctg aagataacat
     9901 gcaagcctaa aggattgagt tatcgcatta tgcccatgaa gccatgtacg cccatataca
     9961 caccgactaa gccaatcaac agactggaaa aatagggggc gcgtttagcg agagtgttaa
    10021 atccgctcca gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg cctacggtga
    10081 caagcgttaa cgccaggcca atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg
    10141 ctttcaactg aatgcatatc aacagcacgg taattgctgc cgggcagggg ataaggccac
    10201 cggttaagcc aaataacaaa atttgccagt tgatgacctc tctaccatca aagcgtcgtt
    10261 taatgtcatt ggcatgggct cgtgcatggg catcctgata ctcgccatgt tcgtgatggt
    10321 gatgatgtcc ttggtcgtgg tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat
    10381 ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt tcgccgcgcc
    10441 aggtacgcca gaacatccag aacacggtgc caataatgat cactgcggaa atcagctgga
    10501 gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg ccaaaggcaa
    10561 tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt
    10621 taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt
    10681 ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt
    10741 gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct
    10801 gattctactc ccccccagta cctgatacta ccccccagta gattaatagt gctatgattt
    10861 ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact
    10921 gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga
    10981 gccgcacgaa tgcgctgcag ttttacaaca gattgctgct atccgtggag cggtaaacgg
    11041 tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga
    11101 gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata
    11161 atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgggt gcgctggaca
    11221 ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt
    11281 gaaggctgag aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg
    11341 accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct
    11401 gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca
    11461 ctgcatgacc aatgatgtgg tgcaaacctt taccgccaat accttgctgg cgctcggtgc
    11521 atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc
    11581 cttgctgatt aacgttggca cactgacgca gccacgcgca caggcgatgc gtgctgccgt
    11641 tgagcaagca aaaagctctc aaacacgctg gacgcttgat ccagtagcgg ttggtgcgct
    11701 cgattatcgc cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg
    11761 taatgcttcg gaaatcatgg cattagctgg cattgctaat ggcggacggg gagtggatac
    11821 cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc
    11881 aatcgtcgtg gtcactggcg aggtggatta tgttaccgat ggacatcgta tcattggtat
    11941 tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt
    12001 tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca
    12061 ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag gcagttttgt
    12121 tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt
    12181 aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattct ggccgatctt
    12241 aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa
    12301 aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc
    12361 gattcggtgt tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc
    12421 gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc
    12481 gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc ggttgctacg
    12541 ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagctgcc
    12601 gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg
    12661 ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa
    12721 agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg
    12781 accaaaaaca cccacggcac tggttgtaca ctctctgcgg cgttggctgc actacgcccg
    12841 cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta
    12901 gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc
    12961 tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc
    13021 agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg
    13081 cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta
    13141 ttcgacagca gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc
    13201 tttcgctatt cgcattcctg ccagttacac cattcacgga ttagatgttt cgcgctggca
    13261 ggagcgtatc gactggcagc gggtggcaaa aatgcgcgac aacgatatcc gcttacagtt
    13321 tgcttttatt aaggctacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
    13381 gcaattaagc cgcgaaaatg gcctgctgcg cggggcgtat cattatttct ccccgtcggt
    13441 atccgcttca gttcaggcgc gattatttct gcaaacggtg gatttctcac aaggcgattt
    13501 ccctgccgtg ctggacgtag aagaacgggg aaaattatcg gcaaaagaat tacgcaagcg
    13561 ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga aaaaagccga ttatttactc
    13621 aggagccgtt ttttatcaca ccaatctggc gggctatttc aatgaatatc cgtggtgggt
    13681 ggctcactat tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
    13741 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
    13801 cacggtggat gagttgcagg cattcgttga tgggattaaa gaaacgcctt aaataagaag
    13861 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
    13921 ccagctgatt gcccaactga acgagcgtat tttagcggcg gataacacgc cgctttatat
    13981 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
    14041 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
    14101 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
    14161 gatcaacaac atcttcgaat actcgttgaa agaagcgaga ggtttttctc agcaggtggt
    14221 attgcgcggg aaaaagcccg atacgctatg ggttaataag cgtgtcgtga aatgtcccga
    14281 agaagttgcg cagcagctgg cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat
    14341 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
    14401 tcatgatgtt gatgccatcg gaatttctct ttatgactat ttccgcagcc agcacattta
    14461 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
    14521 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
    14581 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
    14641 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
    14701 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
    14761 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
    14821 atgcccgaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
    14881 gaacgatcaa cggcgcggca aatttctcct gccattgctt gccaagtgtt gttatctctg
    14941 cagataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagt gacactaaag
    15001 gtcgacaggc catgatccgc gccagtaatg cgtccgggat atcgccaata cgtgggccaa
    15061 aatcgataaa cggcgtcacg tcttgcagct tttccagcca ttccactaac aattcgccgc
    15121 agggcgaggc cagttgataa ccggaaaaat agagcaggtt gccaggcgca acggttaatc
    15181 gtgccaacca ttggcgattc cattgatttt ccacaccgct gaatgacata aaagtgcgtt
    15241 cgccatccgg ctcaaccagt gccagacacc agccgttatc gctttcggcg ttatcgatca
    15301 ggctgattaa cccctctttc gccatccggt tgcgaataat ctccgcccac acgccctgac
    15361 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
    15421 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
    15481 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
    15541 gacgcgtggt taattcaggc agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg
    15601 ttgttgacga tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
    15661 atccagctcc gcctttaacg caggatcgac agcgttaatg ccatgcaacg cgccgcaaat
    15721 cgccgtcgcc atagcaccta tggtgtctgt gtcgccgcca agattggcgc acaggacggc
    15781 gcagcggttt gggtcggttt gtgccagttc aactagcgca atggcgcacg gaacggactc
    15841 aatagtgctg gtgcctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
    15901 gtcggcatta cgcacaattt tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa
    15961 cgtggtggtg cgtttttgct gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc
    16021 tgaccagcgt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
    16081 aaccgccaga tcggatttat gggtcgggct ggacgccagc gccacatcat caataaagga
    16141 atcaacatca cgcgctggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
    16201 ggttacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
    16261 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc
    16321 gagaatatta cgcccaatca gattcggatc gatcttgcct tcacattcca gtaacgcatc
    16381 cgccagacac aggccatcga ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc
    16441 gcgttattct cctttggtcc aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg
    16501 cggctgcgtg gccaaagctc ggagggcatc cccatcgcat cccctaacgc ctgcccataa
    16561 agagcaccga gaatacgttc tgttttcatt taacttcccc ttgtgtcaac gcaatatctc
    16621 gatcatcgac cttgatagcc gtaatttcgt tgtcggattc gcggaaaaaa atcatgaaca
    16681 gcacggcgat aatggcaatc atcaccgcgc cgaaagtcca catcccggac cagttgaaag
    16741 tcagtccgtt taccggttcc tgataagcga acatcttttc catcatcaca ccgccaagac
    16801 gatagccgag caaactgccg aagccctggc agcagagcgt gatcagcccc tgcgcagcgg
    16861 tacgcatatg cacgggggct tttttatcga catagatgta agcggtaacg tagtaaaaat
    16921 cgtaacttac gccgtgaagc aaaataccga ggaacagtaa cgcgtaggtg aaattgacag
    16981 gtaatgtaag gtacgcgatg acaataccac ataataatac cttttgaata cctttgcggt
    17041 gaaaacttga ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa
    17101 aacatccaaa agatggaaaa actcgcccgt aacgaccagg attatgattg cccgcaaaag
    17161 gcgggccagg acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac
    17221 agatattgcg cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac
    17281 tattccttct gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga
    17341 caataatcgc ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct
    17401 ggctggcaca ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg
    17461 agcttcattt gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat
    17521 cgaagcgggc tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg
    17581 ctatgcgcat cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc
    17641 gaatacctac gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt
    17701 ggcggttggt gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat
    17761 ttctgcggcc tttgaacgcg cgcacgagct gggcatggtg gcagtgctgt gggcctattt
    17821 gcgaaactcc gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg
    17881 tcaggcgaac catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga
    17941 aaataacggc ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa
    18001 gttaaccagc gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg
    18061 ccgggctggg ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc
    18121 agtgcgtact gcggttatca acaaacgcgc aggcggaatg gggctgattc ttggacgtaa
    18181 ggcgttcaag aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc aggacgttta
    18241 tctcgatagc aaaattacta ttgcctgata cgcctcatac gaacagcacc accccgtagg
    18301 acggataagg cgtttacact gcatccggca acctatgcct gatgcgacgc ggaagcgtct
    18361 tatcatgcct acatagcact gccacgtatg tttacaccgc atccggcata aaaacacgcg
    18421 cactttgcta cggcttccct atcgggaggc cgtttttttg cctttcactc ctcgaacaat
    18481 tttcatattg tcgtttttgt gatcgttatc tcgacattta aaagcaaata atttcattat
    18541 attttgaaat cgaaagcaaa cgacaagata tgaaaatgta cgtggtatcg acaaagcaga
    18601 tgctgaacaa cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg
    18661 aaacgatgca agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg
    18721 gaacgcctgg cacatttact catgctggta cagaaaatct gttggcgctg gtcagcgcga
    18781 tggcgaagca atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg
    18841 atatcgctca gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc
    18901 cttttgcgca aaatatttca cggctcaaag aggtggtgga tttttgccat cgctttgatg
    18961 tcagcgtcga ggcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg
    19021 aagccgatgc gttgtacacc aacctcgcac aggcgcgtga atttgccgag gcaaccggga
    19081 ttgattccct ggcggtcgcc atcggcacgg ctcacggtat gtatgccagc gcaccggtgc
    19141 ttgatttttc tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg
    19201 gcgcgtcagg attatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa
    19261 tcaacgttgc aacggagctg aaaaatgcct tctcgcagtc gttaaaaaat tacctgaccg
    19321 agcaccccga agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg
    19381 atgtggtgag caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc
    19441 atttaacaag gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc
    19501 ggcatatgtt cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat
    19561 cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc
    19621 ggttataccg gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa
    19681 gttgggtttg cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg
    19741 cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt
    19801 cgtgctggct tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata
    19861 ccgttagcac cagaaacggt tgcggaacga gctgttgtgc tttgctttgc tgcggaaagt
    19921 gtggcgacag attgccagcg tgagcaactg agctatgtca ttggcactga agttccggtt
    19981 ccgggcggtg aggccagcgc cattcagtca gtacacatca cccatgttga agatgccgcc
    20041 aatactttac gtacgcatca aaaggccttt attgcccgtg ggctgacaga ggcgttaaca
    20101 cgtgtgattg ccatcgtggt gcagccgggt gtggaatttg atcacagcaa tattatccat
    20161 tatcagccgc aggaagcgca ggcgctggcg caatggatag aaaacacccg aatggtttat
    20221 gaagcacatt ctaccgatta ccagacccgg acggcttatt gggaattagt ccgcgatcac
    20281 tttgcaatat tgaaagtcgg tcccgcatta acctttgctt tacgcgaggc gatatttgca
    20341 ctggcacaaa ttgagcagga acttatcgcc cccgaaaatc gcagcggttg cctggcggta
    20401 attgaagaag tgatgctcga cgaaccgcaa tactggaaaa aatattatcg cacgggtttt
    20461 aacgattcat tactggatat tcgttacagc ctgtcggatc gtattcgtta ttactggccg
    20521 catagtcgga ttaaaaatag cgttgaaacg atgatggtga atctgcaagg cgtggacatc
    20581 ccactgggca tgattagtca gtatcttccc aaacaatttg aacgcattca gtccggggaa
    20641 ttatcagcaa tgccgcatca gcttattatg aataaaattt atgatgtttt gcgcgcctat
    20701 cgctacggct gtgcggaata aggacggtat atgactaacc tgtttgttcg tagcggaatt
    20761 tcttttgtcg atcgtagcga agttttaacc tatatcggta atgagatgct cgcgaaaggt
    20821 gtggttcatg atacctggcc acaggcatta attgccagag aagcagaatt ccctaccggg
    20881 ataatgcttg agcagcacgc cattgcaata ccgcattgtg aggcgattca tgctaagtcg
    20941 tcagccattt atctgttaag gccaacaaat aaagttcatt ttcagcaagc ggatgatgat
    21001 aacgacgtgg cggtatcgtt ggttattgcg ttgattgtgg aaaatccgca gcagcaattg
    21061 aaacttttac gctgtttatt tggcaagtta caacagcccg atatcgtcga gacactaatt
    21121 actcttcctg aaactcagtt aaaggaatac ttcacaaagt atgttttaga ttcagacgaa
    21181 taaatccctc tgtaacaata aaaaggacta tttatgaaac gcaagattat tgtcgcttgc
    21241 ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa ttaaagagtt gtgtcagagt
    21301 cataatattc ctgttgaatt aatccagtat cgggttaatg aaatagaaac ctatatggat
    21361 ggtgtgcatt tgatatgtac cactgccagg gtggatcgta gttttggcga tattccgtta
    21421 gttcacggca tgccttttgt ttctggtgtc ggtatcgaag cattacaaaa taaaattctg
    21481 actatcttac aggggtgacc tatgttttca gaagtcatgc gttatattct cgacctcggc
    21541 cctacagtga tgctgccggt tgtcatcatt attttttcta aaatattagg catgaaggca
    21601 ggcgattgct ttaaagcggg tctgcatatc gggattggct ttgttggcat tggccttgtg
    21661 attggcttaa tgctggattc cattggtccg gcggcgaaag cgatggcgga aaatttcgac
    21721 ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt caccaatgac ctgggcgtcg
    21781 caaattgcgc tggtggcgat tccgattgcg atattggtta acgtggcgat gttactgacc
    21841 cgtatgacgc gggtggtaaa tgttgatatc tggaatatct ggcatatgac cttcaccggc
    21901 agcgttgctg cacctggcaa ccggttcatg gatgataggg atggcgggtg tggtaattca
    21961 cgcggcgttt gtttataagc tcggcgactg gtttgcccgc gatacccgaa atttctttga
    22021 gctggaaggt attgctattc cgcacggtac gtcggcgtat atggggccga ttgcggtgct
    22081 ggtcgatgct atcatcgaga aaatcccagg cgttaaccga attaaattta gcgccgacga
    22141 tattcagcgc aaatttggcc cttttggcga acctgtcacc gtgggttttg tgatggggct
    22201 gattatcggc atcctcgcgg gttacgatgt caaaggtgta ttgcagctgg cggtaaaaac
    22261 ggcggcagtt atgctgctga tgccacggat gattaaaccc atcatggatg gtttaacgcc
    22321 catcgctaag caggcccgta gtcgtttaca ggcgaagttc ggcggtcagg agttcctgat
    22381 tggcctggat cccgcattac tgctggggca tacggcggtg gtatcggcaa gcctgatttt
    22441 tattccgctc accattttaa ttgctgtttg tgttccgggt aatcaggtac tgccgtttgg
    22501 cgatcttgcc actatcggct tcttcgtggc gatggcggtc gcggtgcatc gtggaaatct
    22561 gttccgcacc ttaatctcgg gtgtcatcat tatgagcatc accctgtgga tcgcgacgca
    22621 aactattggt ttgcacaccc aactggcggc taatgctggg gcgttaaaag ccgggggtat
    22681 ggtggcttca atggatcagg gcggttctcc cattacctgg ttactgattc aggttttctc
    22741 cccgcaaaat attcccggtt tcattattat cggcgcaatt tatctgaccg gtattttcat
    22801 gacctggcgt agagcgcgtg gttttactaa acaagagaaa gccgttctcg cagaataatt
    22861 tttacctgag ggggaggtta tcccccttaa caatcaggag tttttatgaa atcagtagtg
    22921 aatgatactg atggtatcgt gcgcgttgca aaaagcgtca ttcctgaaat taaacatcag
    22981 gatgaggtgc gggtaaaaat tgccagctcg ggcttatgtg gttccgattt acccaggata
    23041 tttaaaaatg gtgcacatta ttatccaata acgttaggcc atgaatttag cggctatatt
    23101 gatgcggtgg gatccggtgt tgatgattta caccctggcg atgcggttgc ctgtgtgccg
    23161 ttattaccct gttttacttg tccagagtgt ttgaaagggt tttattccca gtgcgcaaaa
    23221 tatgatttta ttggctcgcg gcgtgatggt ggatttgctg aatatattgt cgttaagcga
    23281 aaaaatgtct ttgctctacc cacggatatg cctattgagg atggggcttt tattgagccg
    23341 attaccgttg gcctgcatgc ttttcattta gcgcaaggtt gtgagaataa aaacgttatt
    23401 attattggtg ccggaaccat tggcctgctg gctattcagt gcgctgtcgc gctgggagca
    23461 aagagtgtga cggcgatcga cattagctca gaaaaactgg cactggcaaa atctttcggt
    23521 gcgatgcaaa catttaacag tagcgaaatg agcgcgccgc aaatgcagag cgttttacgc
    23581 gaactgcgct ttaatcagct tatcctcgag acggctggcg taccgcaaac tgtcgaactg
    23641 gcggtagaga ttgccggtcc tcatgcccaa ctggcgctgg tgggcacgtt gcatcaggat
    23701 ctgcatttaa catcggcaac gtttggcaaa atattgcgta aagagctgac ggttatcggc
    23761 agttggatga actattccag cccttggccg gggcaggagt gggaaacggc gagccggttg
    23821 ctgacagaac gtaagttaag cctggatcca ttaatcgctc accgtggaag ctttgaaagc
    23881 ttcacccagg cggtgcgtga catcgctcgt aatgccatgc cgggcaaagt gttgctcatt
    23941 ccatgaaacc gcgggccagc gtgatgctgg cccggtattg tgcaaaacag atcattcacc
    24001 aatggtcccc cttcgtttac actagccaca attgaatatg ggtaaatgac gatgaattca
    24061 ttcgagcgaa ggaataagat catccaatta gtgaatgaac agggaaccgt gcttgttcag
    24121 gatctggcgg gagtatttgc tgcctcggaa gcgacaatcc gtgccgattt gcgctttctc
    24181 gaacaaaaag gcgtggttac gcgctttcat ggcggtgcgg cgaaaataat gtccggtaat
    24241 agtgaaaccg agacccagga agtcgggttt aaagagcgat ttcagctcgc cagcgcgcca
    24301 aaaaacagaa tagcgcagga ggcagtcaaa atgatccatg aagggatgac cgttattctc
    24361 gacagcggaa gtaccacaat gcttatcgcc gaaggattaa tgaccgctaa aaatatcacg
    24421 gtgattacca acagtctccc ggcagcgttt gccctttccg aaaataaaga cattactctg
    24481 gtcgtctgtg gtggcacggt ccgccataaa acgcgctcga tgcatggttc cattgccgag
    24541 cgttcattgc aggatattaa tgccgattta atgtttgtcg gtgctgatgg tattgatgcg
    24601 gttaatggta ttacgacctt taatgaaggt tattcgatta gcggggcgat ggtcaccgcc
    24661 gcaaataaag tgattgccgt tctcgattca tcgaaattta atcgtcgcgg ttttaatcag
    24721 gtactgccaa tcgaaaaaat tgacattatt gttaccgatg atgctgtgtc agaggtggat
    24781 aaattggcat tgcagaagac acgggtaaaa ttaattacgg tgtagtattg ttctgtatgc
    24841 cggataaggt gtttacaccg catccggcat tacggattct ccctggttca agagagaata
    24901 gtttgattaa cgcaataatg gacaatctgg tggtaatcga caacgcaacg ccgccggaag
    24961 tatttcaata tggaagtttt gcccactcaa cggttcgcca tcaagattaa aagtgatttc
    25021 gtgtggggct tgtatatcaa accacgacga agcgccttcg ataatattcg ggttatcttc
    25081 gtcagatttt aaggttgata cgagagccgg aagaatttca tcgccggtaa aaatgcgcag
    25141 ttgcagcaag ccatcgttaa ttaacgcgtt cgggcacaat tgttgaccgc caccggcctg
    25201 acgcccgtta ccaataccaa tgaccagggc gtcaccttgc cagtgaaagt tttcaccgcg
    25261 gatttcacaa cggtccggtt gcagagtatc catgcgcatt aagccatgaa tgatgtaaga
    25321 gacgccaccc agcgcggctt ttaatttttc cggcgtttct gtggtaatac gcgtcccaaa
    25381 tccgcctgtc gccatattaa taaaacaggt ttgtttgttg acctgcgcca tatctatcgc
    25441 aatggcatta ccggcaattg ccagtttcag cgccttatcc agtgcctcag gaatccctac
    25501 actggtggca aaatcattgg cggttcctaa tggcaaaatt cccagcgcgg gtatgtcatc
    25561 cccctcacac tgaatcaacg ccgtagaaac ttcattaatg gtgccatcgc caccaccggc
    25621 aatcaccgtt gcgacgccca acttccgggc ctcctctaca aatcgtgcgg catcaccttt
    25681 ctcccaggtg acccgcacat ggatcgtcat tccttcctca cgcaacagca taattgcttc
    25741 gcgcaagggt agattgtcag tacttttgcc attaagaatc agtaagctgg cgggaaattc
    25801 tgccatgatc gtatgtgcct ttatgattgg tctgtagata gtgtagagca gaaaacaaaa
    25861 ggtggagtca gaacaggatg aaagtcggag gataaaaagt cagttcaagt atggaggatt
    25921 cagtggttac caccaggagc ttgcaatggg gacagcgaat actaacggct gccaccgata
    25981 aatttcaaaa aagagcatat acctaatatt caactaaaca gtggcatctt caatataata
    26041 tattaaagcc cccatggagt taccctgaag ggcctcaatg tccgtaattc ctacttatgt
    26101 aggaaatgtt gtacagaaca tttattataa tcctattcaa ttataataat catgccatta
    26161 ttatatttaa acactagaga gtgtcgttgg tatttaatgg gggaaggtga gatgaaaaag
    26221 atagctgcta tatcattaat tagtattttt cttatatctg gttgtgctgt gcataatgat
    26281 gaaacaagta tcggtaaatt tggtcttgca tataaaagta atattcagcg taaactcgat
    26341 aaccaatact acaccgaagc cgaagcttct ttagccaggg gcagaatatc tggtgcagaa
    26401 aatatagtaa aaaatgatgc agcccatttc tgtgttactc agggcaaaaa aatgcagata
    26461 gttgacctga agacagaagg tgcaggatta catggcgtcg ctcgtctgac attcaaatgt
    26521 ggagagtgag aatatttttt ggtaagcgtc aaacatgcgc gttctggctg tgcgtagccg
    26581 gaacctgggc gagcacgata ccgttacgtg aaaggcattg tgctatgaag ggggattcta
    26641 tcgatgtggt caatggaaga cggttatcag ggatcaatcc ttctttacag cttatgtaca
    26701 aaaaataagc ccgtgtaagg gagattacac aggctaagga ggtggttcct ggtacagcta
    26761 gcattttatg gattatgttt ttcagcgaaa cggatgataa ccttaataaa tgcagctgta
    26821 tgtgatcggt ttctaagaat tttccatccg ggaaaaataa tcgaaattaa tcacttaccg
    26881 tgggggttac gcgtggtttc cccggagaaa ttacgcatta gcagcgcgta atttagctca
    26941 agatcctgcg ggaccgggag ccacacagta taaccatcgc ctggtgcgac cggcatagct
    27001 tcgcctttgg cgttttccat gtgctcaagg gtaaagttaa tgttgccttg cggcgtcatc
    27061 agctcaagac tgtcgccaac ggagaattta tttttcaccg ctaccgctgc gaggtccccc
    27121 ttgcgctcac cggtaaactc accaacaaac tgctggcggt cagaaactga ataaccgtat
    27181 tcgtagttct gataatcgtc gtgagtatga cgacgcagga aaccttcggt atagccacga
    27241 tgcgccagac cttccagagt ttccagcagg ctggtatcga acggttttcc cgcagcggcg
    27301 tcatcgatag ctttgcggta aacctgtgcg gtgcgtgcgc aatagtagaa agatttggta
    27361 cgaccttcga ttttcagcga atgcacgccc attttggtca ggcgttctac atgggcgatg
    27421 gcgcgcagat ctttcgagtt catgatgtaa gtgccgtgct catcttcaaa cgcggtcata
    27481 tactcgcccg gacgctgggc ttcttcgatc ataaacactt tgtcggttgg cgcgccgata
    27541 cccagcgtcg gctcaacatt ttgcaccgga atcggctcgt acttgtgtac gatgttgcca
    27601 acgtcatctt ctttcccttc ctggacgttg tactcccagc ggcaggcgtt ggtgcaggtg
    27661 ccctggttcg ggtcgcgctt gttgatatag ccagagagca ggcagcgacc ggagtaggcc
    27721 atgcacagtg cgccgtgaac gaagatctcg atctccatat ccggcacctg attatggatc
    27781 tcttcaatct cttccagcga cagctcgcga gagaggatca cgcgggtcag tcccatttgc
    27841 tgccagaatt tcaccgtcgc ccagttcacg gcgttagcct gcaccgagag gtggatcggc
    27901 atttctggga agtgctcacg caccagcata atcagccctg gatcggacat aatcagcgca
    27961 tccggcccca tttccaccac cggtttcagg tcacggataa aggttttcag cttggcgttg
    28021 tgtggtgcaa tgttgaccac gacataaaac tttttcccca gcgcgtgggc ttcattgatg
    28081 ccgagctgaa gattttcgtg gttgaattcg ttgttgcgca cacgtaggga gtaacgcggc
    28141 tggcccgcat aaacagcatc tgcgccataa gcgaaagcgt aacgcatatt tttcagcgtt
    28201 cccgccgggg aaaggagttc cggtttaaac ataattctct cgttctgatg acaggtcaga
    28261 tccgcttcac ctgatgaagc ggttagggga ggcccccact ttaagggcgg gcattgtagc
    28321 gctgtagggg ggatctggta aagcgccgga cgccaacaac ccacgcccgg catcgtaatt
    28381 acgtattgtc tttcaccgtc tggcttgtgc cattggcttt aacggaggca attccggttt
    28441 cacgagattg cgcggtggcg tacatctggc tggagccgat aatttgatga ttagcggctt
    28501 tcagattgaa atagaatttg ccgttttttt ctcatagcgc tcttcttgcg ggctgttgct
    28561 acgcaccgac gcgatgccct tttccgcaga ggcttttgag gtatacagct cgctggtgag
    28621 gatagtctca ccattgcccg cttttagcac aaaccggaac tgattatcac tgctcttact
    28681 gagttcaaac caaccagcca taatgactcc ttgattgaaa ttgcttatcg aaaacagcat
    28741 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
    28801 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
    28861 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
    28921 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
    28981 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
    29041 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctgat tcgtgagaga
    29101 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
    29161 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
    29221 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
    29281 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
    29341 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
    29401 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
    29461 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
    29521 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
    29581 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
    29641 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
    29701 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
    29761 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
    29821 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
    29881 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
    29941 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
    30001 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
    30061 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
    30121 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
    30181 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
    30241 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
    30301 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
    30361 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
    30421 atcgataagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
    30481 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
    30541 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
    30601 cgcaccgttg accagaatgg gtcgtcgtgt accgtcaggt ggaatcggcg ctcgcggacc
    30661 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
    30721 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
    30781 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
    30841 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
    30901 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
    30961 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
    31021 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
    31081 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
    31141 tgtgtggtgc cgctgtcgac gctgacatgc tgcgaaccaa aaagccccag caacagcccg
    31201 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
    31261 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
    31321 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
    31381 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
    31441 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
    31501 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
    31561 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
    31621 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
    31681 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
    31741 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
    31801 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
    31861 gtgtagtttg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
    31921 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
    31981 agtagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
    32041 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
    32101 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
    32161 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
    32221 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
    32281 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
    32341 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
    32401 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
    32461 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
    32521 agctggctca tcaccagacc gccgacaatg gtgatcctca ggggttgccg cagctccgag
    32581 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
    32641 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
    32701 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
    32761 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
    32821 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
    32881 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
    32941 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
    33001 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
    33061 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
    33121 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
    33181 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
    33241 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
    33301 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
    33361 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
    33421 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
    33481 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
    33541 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
    33601 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
    33661 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
    33721 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
    33781 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
    33841 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
    33901 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
    33961 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
    34021 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
    34081 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
    34141 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
    34201 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
    34261 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
    34321 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
    34381 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
    34441 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
    34501 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
    34561 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
    34621 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
    34681 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
    34741 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
    34801 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
    34861 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
    34921 gggttcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
    34981 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
    35041 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
    35101 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
    35161 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
    35221 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
    35281 agttagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
    35341 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
    35401 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
    35461 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
    35521 cgacttttgg tccacaatac caagcggtcg aacagcagat aaatcaccgg cgtggtgaac
    35581 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
    35641 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
    35701 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
    35761 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
    35821 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
    35881 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
    35941 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
    36001 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctgg
    36061 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
    36121 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
    36181 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
    36241 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
    36301 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
    36361 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
    36421 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
    36481 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
    36541 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
    36601 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
    36661 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
    36721 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
    36781 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
    36841 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
    36901 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
    36961 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
    37021 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
    37081 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
    37141 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
    37201 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
    37261 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
    37321 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
    37381 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
    37441 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
    37501 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
    37561 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
    37621 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
    37681 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
    37741 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
    37801 tctacagttg ctacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
    37861 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
    37921 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
    37981 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
    38041 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
    38101 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
    38161 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
    38221 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
    38281 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
    38341 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
    38401 acggcagagg tcatgacatc cgggctggca cttgggtaga gcgtgaccac ctgaatggtc
    38461 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
    38521 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
    38581 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
    38641 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
    38701 ctttctgact gtcctgaatg cccggcgtca ccagatgttt gctgaccttg ttttcgctat
    38761 tcagcaccca gacaaaatgg ccttcattgc ccatttgcag ggcggctgtt gggatcacta
    38821 cggcgttttg ttcggtgtcg actaacatgc gcgcgttaac aaactgattg ggaaacagcg
    38881 catcatcctg attattaaag cgtgctttca ctttaatcgt accggttgtg gcatcgattt
    38941 ggttatcgag gcttaacagc gtgccttcac ttaatttctt cgaattggtg cgatcccagg
    39001 cttctaccat cagcggtttt ccggcttttt gcgcctgcac tacagtagcg atatcgcttt
    39061 ccggcagggt aaagactaaa tcgataggat gcgtctgggt gatcaccaca attccggtgg
    39121 tatcaccact ggagatttgg ttaccaacat caacttgctt gagaccaacg cgaccatcga
    39181 ctggtgcggt gatgcggctc cagtcgagtt gcaactgcgc gctggcgacg cttgcttcat
    39241 cagccttaat ggtgccttcg gtttcactga ccagcgcctg ttgggcatcc agctcctgac
    39301 gggagacgag attggttttt gccagttgct gataacgcgc cagatcacgg cgggcgttag
    39361 caagcgtggc tttatctttt gccagttggc cctgggtttg tgctaatgca actttgaact
    39421 ggctggggtc aatttctgcc agtaaatcgc ctgctttgac ctgctggcct tcctggaaat
    39481 gtaacgccat cagttgaccg tccacgcggc tgcgcaccgt aacagtatta gcggcggtaa
    39541 tggtgccaag cccggt
//
LOCUS       DADLXX010000024        37628 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLXX010000024 DADLXX010000000
VERSION     DADLXX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.024"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..174)
                     /locus_tag="JNX27_001234"
     CDS             complement(<1..174)
                     /locus_tag="JNX27_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7882502.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQYR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNX27_001235"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNX27_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7882503.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNX27_001236"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNX27_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7882504.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNX27_001237"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNX27_001237"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7882505.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNX27_001238"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNX27_001238"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7882506.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNAVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNX27_001239"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNX27_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7882507.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="JNX27_001240"
     CDS             complement(5178..5420)
                     /locus_tag="JNX27_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7882508.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNX27_001241"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNX27_001241"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7882509.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNX27_001242"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNX27_001242"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7882510.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="JNX27_001243"
     CDS             complement(8337..9311)
                     /locus_tag="JNX27_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7882511.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="JNX27_001244"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="JNX27_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY7882512.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVIEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /locus_tag="JNX27_001245"
     CDS             11708..12751
                     /locus_tag="JNX27_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7882513.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="JNX27_001246"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="JNX27_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7882514.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="JNX27_001247"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="JNX27_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7882515.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="JNX27_001248"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="JNX27_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7882516.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="JNX27_001249"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="JNX27_001249"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7882517.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /locus_tag="JNX27_001250"
     CDS             16537..20337
                     /locus_tag="JNX27_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7882518.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="JNX27_001251"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="JNX27_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7882519.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNX27_001252"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNX27_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7882520.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="JNX27_001253"
     CDS             23178..23381
                     /locus_tag="JNX27_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7882521.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="JNX27_001254"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="JNX27_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY7882522.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="JNX27_001255"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="JNX27_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7882523.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGMLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="JNX27_001256"
     CDS             26386..26658
                     /locus_tag="JNX27_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882524.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JNX27_001257"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JNX27_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7882525.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JNX27_001258"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JNX27_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7882526.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="JNX27_001259"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="JNX27_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7882527.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JNX27_001260"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JNX27_001260"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7882528.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="JNX27_001261"
     CDS             complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="JNX27_001261"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY7882529.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31926..32324)
                     /locus_tag="JNX27_001262"
     CDS             complement(31926..32324)
                     /locus_tag="JNX27_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7882530.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32518..33645
                     /locus_tag="JNX27_001263"
     CDS             32518..33645
                     /locus_tag="JNX27_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7882531.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33864..34292
                     /gene="rplM"
                     /locus_tag="JNX27_001264"
     CDS             33864..34292
                     /gene="rplM"
                     /locus_tag="JNX27_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7882532.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34308..34700
                     /gene="rpsI"
                     /locus_tag="JNX27_001265"
     CDS             34308..34700
                     /gene="rpsI"
                     /locus_tag="JNX27_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7882533.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35095..35733
                     /gene="sspA"
                     /locus_tag="JNX27_001266"
     CDS             35095..35733
                     /gene="sspA"
                     /locus_tag="JNX27_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7882534.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35739..36236
                     /gene="sspB"
                     /locus_tag="JNX27_001267"
     CDS             35739..36236
                     /gene="sspB"
                     /locus_tag="JNX27_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7882535.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36279..37541)
                     /gene="dcuC"
                     /locus_tag="JNX27_001268"
     CDS             complement(36279..37541)
                     /gene="dcuC"
                     /locus_tag="JNX27_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7882536.1"
                     /translation="MMCGVWLGFGGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLS
                     IMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYIILSATYVIGQIMAQFITSASGL
                     GMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGMKIATYFFH
                     YQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLI
                     LMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGT
                     QFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMG
                     SGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVS
                     PFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9110 a         9412 c         9904 g         9202 t
ORIGIN      
        1 tctgtactga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgcggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaga ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac ggcattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac gagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagtgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga cgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accctggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatagaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgtg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gagccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcaccgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg acatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttggcggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cctaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg atgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccttt
    26461 ggtctggcta aagataaggt acgcgatctc gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaaa gcccccgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtacacac taattattga
    27061 actaattaat accacgctaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcgaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggcgtg
    30661 atcatttcag cgctggccga cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt
    30721 ggtttgccgt tacgcagcag gccaagcttc actttcgtgc ccggctctgt agtcgcgata
    30781 cgagagcgca actcagcaaa gctattcagc ggtttgccgt tgaggctggt aataatatcg
    30841 cccgctttga cgcccgcttt cgctgagcca gaacttggca acacttcgct gacaaacgcg
    30901 ccacgctgca cgtcaaggtt gaaggctttg gcgatatcgg cactcatctc ggtgcctttg
    30961 atgcctaaca aaccgcgttt gatttcacca aagtcgataa gctgctgcgc cagtgttcgc
    31021 gccatattac tggggatggc aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt
    31081 gcagtgttga tgccaattaa ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg
    31141 cggttaatgg aagcatctgt ctggataaag ttttccagac cttcaagatt caacccgctg
    31201 cggcctaatg cggaaacaat gccagaggtg gcggtttgcc caaggccaaa tgggttaccg
    31261 accgctacgg caaaatcacc gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt
    31321 aatttgctcg ggttttgaat ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc
    31381 agttttgcat caaactcgcg cccatcattg agctgaatac tgattttctg tgcctgatta
    31441 atcacatggt tgttggtcag cacatagcct ttactggcgt tgatgatgac accggaacct
    31501 aaaccttcga agggttgtgc aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat
    31561 tcttccggga ttttctgtcc ctgactggcc gttccttcca cccgtacgct caccactgcc
    31621 ggaagcactt tttccagcat tggagccaga ctggggagag gggcctgatc ggcaacctgg
    31681 cctggaatcg acgcgacggc ctgaaatgac gccgagagag ttaacccggc acttaacgct
    31741 aatgcactca acagctgggt ttgttttttc attattcctg ctctcgtacc tgaatgataa
    31801 gaaaagagat tcaaaacagt ttgatgttat tgaattttca gacggctaac aatgagacac
    31861 cagattaaat gatagtcggg gagggagaga agaggagggc gcaatggctg cgcccgaaaa
    31921 ataaattagt cgcgcttcgc gccagtacgc agcaggccgg atgcgccttc agaatagtcg
    31981 cgaggcatct gcaccggtgc ctgatcgttg ctggcttcag actctgccag acgattacgg
    32041 aacgggtttg cttcagcaga cagttccggc agcaggctgc tggagctttt tgccatgtgc
    32101 tgatacagct ggcgatagtc gtgcgccatg gtatccagta attccgcgct gcgggcaaag
    32161 tggctaacca gctcttcgcg atactcgtcc agttcagctt tattcttttc cagttcgtac
    32221 tgcaacgcct gttgctggcg tagtttacga ttaccaaaac gcatggccac agcaccaata
    32281 atgatgccga cgactaaccc aattagcgca tattcccagg tcatgaacat ctcccgttgt
    32341 cttttgtttc cgtagggtgt tggcttcagg ctccatgcct gcggctgatt atgccactat
    32401 aaccgttaat tccacagaag tggaatcccg actgcatatc gcgtagtgta gaacggcctt
    32461 tttttcgtca accgtgaaca acggcgcacc gattattcaa ggaataacaa taagatcatg
    32521 caaagcgtta ccccaacatc gcaatacctg aaggcgctca atgaaggcag ccatcaaccc
    32581 gacgacgttc aaaaagaggc cgtcagccgc ctggaaatta tttatcaaga actcatcaat
    32641 agcaggccac cagcccccag gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt
    32701 aaacgcgaag acacaaagca tacgccagtg cgtggcttat atatgtgggg cggtgtagga
    32761 cgcgggaaaa cctggctgat ggaccttttc tatcaaagcc tgccgggaga gcggaaacag
    32821 cgcctgcact ttcaccgttt tatgctgcgg gtgcacgaag agctaactgc cttacagggg
    32881 cagaccgatc cgctggaaat tattgccgat cgctttaaag ccgaaactga cgtgctctgt
    32941 tttgacgaat tttttgtttc tgatattacc gacgccatgc tacttggcgg tctgatgaaa
    33001 gccctgttca cccgcggtat taccctggta gcgacgtcaa atattccgcc ggacgaactt
    33061 tatcgaaatg gcctgcaacg tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat
    33121 tgtgatgtaa tgaacgtgga cgctggtgtt gattatcgac tgcgtacact cactcaggcg
    33181 catctgtggc tttcgccact caacgatgaa acccgggcgc agatggataa actatggttg
    33241 gcgctggcgg gggtgaaacg agaaaattca ccgacgttag aaatcaacca tcggccattg
    33301 gcgacaatgg gcgtcgagaa ccagacgctg gcggtttctt ttactacgct gtgcgtcgac
    33361 gcccgcagtc agcatgacta tattgcgctc tcacgcctgt ttcacacagt aatgttgttt
    33421 gatgtgccag tcatgacgcg gttgatggag agcgaagcgc ggcgctttat tgcgctggtg
    33481 gatgagtttt acgagcgcca tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa
    33541 atttatcagg gtgagcggct gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag
    33601 atgcaaagcg aagagtatct gaagcgcgag catttggcgg gttaaaacct gccacaaatc
    33661 acaaaaaggg gtcgatcttt gaccccgact tctctataat cctgcgaccc cacgttacaa
    33721 gaaagttttt ttcccaaaac tttttgtgtg ctggcatagg ctattcgaag gggtaggttt
    33781 gccggacttt gtcgtgtgaa cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta
    33841 actatttatt gggtaagctt ttaatgaaaa cttttacagc taaaccagaa accgtaaaac
    33901 gcgactggta tgttgttgac gcgaccggta aaactctggg ccgtctggct actgaactgg
    33961 ctcgtcgcct gcgtggtaag cacaaagcgg aatatactcc gcacgtagat accggtgatt
    34021 acatcatcgt tctgaacgct gacaaagttg ctgtaaccgg caacaagcgt actgacaaag
    34081 tgtactatca ccacaccggc cacatcggtg gtatcaaaca agcgaccttt gaagagatga
    34141 ttgctcgccg tcctgagcgt gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc
    34201 cgctgggtcg tgctatgttc cgtaaactga aagtttacgc gggtaacgag cacaaccacg
    34261 cggcacagca accgcaagtt cttgacatct aatcgggatt ataggcaatg gctgaaaatc
    34321 aatactacgg cactggtcgc cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca
    34381 acggtaaaat cgtaatcaac caacgttctc tggaacagta cttcggtcgt gaaactgccc
    34441 gcatggtagt tcgtcagccg ctggaactgg tcgacatggt tgagaaactg gacctgtaca
    34501 tcaccgttaa aggtggtggt atctctggtc aggctggtgc gatccgtcac ggtatcaccc
    34561 gcgctctgat ggaatacgac gagtccctgc gttctgaact gcgtaaagct ggcttcgtta
    34621 ctcgtgacgc tcgtcaggtt gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc
    34681 cgcagttctc caaacgttaa ttggcttctg ctccggcaga aaacaatttg cgaaaaaacc
    34741 cgcttcggcg ggttttttta tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct
    34801 ttttgctgcc gtctgaaaga caggtaaaac aaaaaccaca tcgccaataa gggtctaagt
    34861 caactatttc agactaaagc gcatctcttt ttccccattt ccggcatcca ctcaccacaa
    34921 aggtcgcaaa atctggtaaa ctatcatcca attttctgcc caaatgtcgg gtattgctca
    34981 ttttttgttt gattttcgaa caaagagagt cgttccttat tgggtaacac aacttctgac
    35041 tggccacctg gtggctggta gcagtaaaaa ttctgactat acctggaggt tttcatggct
    35101 gtcgctgcca acaaacgttc ggtaatgacg ctgttttccg gtcctactga catctatagc
    35161 catcaggtcc gcattgtgct ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa
    35221 aaggacaatc cgcctcagga tctgattgac ctcaacccga atcagagcgt tccgaccctg
    35281 gtggatcgtg agctgaccct gtgggaatct cgcatcatta tggaatatct ggatgagcgt
    35341 ttcccgcatc cgccactgat gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac
    35401 atgcatcgca tcgaaaaaga ctggtacacg ctgataaaca ctatcatcaa cggttcagct
    35461 tctgaagcag atgccgcacg taagcaactg cgcgaagaac tgctggcgat tgcgccggtc
    35521 ttcggtcaga agccgtactt cctgagcgat gagttcagcc tggtcgattg ctaccttgct
    35581 ccgctgctgt ggcgtctgcc gcaactgggc atcgagttca gcggcccggg tgcgaaagag
    35641 ctgaaaggct atatgacccg cgtctttgag cgtgactctt tccttgcttc tttaactgaa
    35701 gcagaacgtg aaatgcgtct gggccggagt taatctgtat ggatttgtca cagctaacac
    35761 cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt gctggataac cagctcacgc
    35821 cgcacctggt ggtggatgtg acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg
    35881 acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg
    35941 atgaggtgcg ctttaacgcg cgctttggtg gcattccgcg tcaggtttct gtgccgctgg
    36001 ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag
    36061 ctgcctacga tgaagatacc agcatcatga atgatgaaga ggcatcggca gacaacgaaa
    36121 ccgttatgtc ggttattgat ggcgacaagc cagatcacga tgatgacact catcctgacg
    36181 atgaacctcc gcagccacca cgcggtggtc gaccggcatt acgcgttgtg aagtaataca
    36241 aaacaggccc aggcggcctg ttttgtcttt ttaatgactt aataaaatag cgtgattgtg
    36301 gcaatcatat tcaccacgaa accgactgcc atggggatcg ctgtccgctt caccaccgca
    36361 aaaggcgaaa cgcctgcaat tcctgacgta acgaccacca ccgcagtaat cggcgaaacc
    36421 gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca ttacaaccgc tggtacatgt
    36481 agtccggctg cgatattcgg aataagactg gcaaatgaca taaacggcgc attgccagag
    36541 cccatcacaa tggcacaaat ggcaatgact agcgccataa taatcatcac gccaataccg
    36601 cccagaccag aatgctccgc acccctgata accgcatcaa cagtgccaat cgtcgttaag
    36661 cctttcgcaa atatttcacc cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc
    36721 atgccgtcaa aaaacgcctg cacatcgtcc attgtttcgc gcaagttatg cttgcggaag
    36781 aactcaacaa acatcgtcac cgtcaaactt agtaacatca ccaccaccag atgcagttct
    36841 gacttcatca gcccgacgtg ggcgaggaac agcgagccga gcatcaggat taacggcatc
    36901 acaggtaaaa tggcgtaata gagcggcggg acattatcga gagttttttg ctctgcctgt
    36961 tcgtgattga tatttttatc ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc
    37021 gccaccgaga taatgacgca agaggcgacc ggaagctggt agtggaagaa gtatgtggca
    37081 attttcattc ccgcgacctg ggctgcaaaa atggagttcg tttccagaat cccccactca
    37141 atggacatcg tggttgcgat aaccgccacc gcagagaggc gacttactcc cagactcacc
    37201 agcgtcggaa ataaggtgac catcagcaac atacccagac cggaggcgct ggtaataaac
    37261 tgcgccatga tttggccgat gacgtaagtt gccgacagaa taatatacgg cgagcgaatg
    37321 agttttaacg ggcggcttag caagctcacc atcgcgcgac tggctcctat gcgctccatg
    37381 tagcgggcat aaccgcccac cgccataatc gacagcccca atccggcaat gcggttggac
    37441 agcatgcgca ggatttcatt ataaatgtcg acgatcaagt agccgctgct tttggcggga
    37501 tcgagtacac caccgaaccc taaccagact ccgcacatca tcaggaagac acctgcggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DADLXX010000025        36607 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLXX010000025 DADLXX010000000
VERSION     DADLXX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.025"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="JNX27_001269"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="JNX27_001269"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7882537.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /locus_tag="JNX27_001270"
     CDS             2619..3380
                     /locus_tag="JNX27_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7882538.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="JNX27_001271"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="JNX27_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7882539.1"
                     /translation="MSSDADAHKVGLIPVILMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="JNX27_001272"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="JNX27_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7882540.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="JNX27_001273"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="JNX27_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7882541.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="JNX27_001274"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="JNX27_001274"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7882542.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNX27_001275"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNX27_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7882543.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNX27_001276"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNX27_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY7882544.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="JNX27_001277"
     CDS             complement(10310..11188)
                     /locus_tag="JNX27_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882545.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNX27_001278"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNX27_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7882546.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="JNX27_001279"
     CDS             13815..14150
                     /locus_tag="JNX27_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7882547.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="JNX27_001280"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="JNX27_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7882548.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="JNX27_001281"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="JNX27_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7882549.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="JNX27_001282"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="JNX27_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7882550.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="JNX27_001283"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="JNX27_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY7882551.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="JNX27_001284"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="JNX27_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY7882552.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVAEALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="JNX27_001285"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="JNX27_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY7882553.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="JNX27_001286"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="JNX27_001286"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY7882554.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="JNX27_001287"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="JNX27_001287"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY7882555.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLVHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="JNX27_001288"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="JNX27_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY7882556.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHVLEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="JNX27_001289"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="JNX27_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY7882557.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="JNX27_001290"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="JNX27_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY7882558.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="JNX27_001291"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="JNX27_001291"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7882559.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNRIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="JNX27_001292"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="JNX27_001292"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY7882560.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="JNX27_001293"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="JNX27_001293"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY7882561.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="JNX27_001294"
                     /pseudo
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="JNX27_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25844..26173)
                     /locus_tag="JNX27_001295"
     CDS             complement(25844..26173)
                     /locus_tag="JNX27_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882562.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="JNX27_001296"
     CDS             complement(26242..26874)
                     /locus_tag="JNX27_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7882563.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="JNX27_001297"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="JNX27_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="JNX27_001298"
     CDS             29284..30804
                     /locus_tag="JNX27_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7882564.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="JNX27_001299"
     CDS             30801..31775
                     /locus_tag="JNX27_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7882565.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="JNX27_001300"
     CDS             31806..32747
                     /locus_tag="JNX27_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7882566.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="JNX27_001301"
     CDS             32802..33485
                     /locus_tag="JNX27_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY7882567.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQGLMLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="JNX27_001302"
     CDS             33500..34360
                     /locus_tag="JNX27_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HAY7882568.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="JNX27_001303"
     CDS             34357..35358
                     /locus_tag="JNX27_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY7882569.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="JNX27_001304"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="JNX27_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36607
                     /locus_tag="JNX27_001305"
     CDS             36352..>36607
                     /locus_tag="JNX27_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882570.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         8526 a        10223 c         9724 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gattacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt catcctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgccgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctgggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgaagcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatggttc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg tgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg tgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aatgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccgccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcgtattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tacaggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gtaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagc gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagcc gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccactcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctcg ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgctgcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaagtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacagg
    33001 ggctgatgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatc
//
LOCUS       DADLXX010000026        35272 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLXX010000026 DADLXX010000000
VERSION     DADLXX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.026"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="JNX27_001306"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="JNX27_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7882571.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="JNX27_001307"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="JNX27_001307"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY7882572.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="JNX27_001308"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="JNX27_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7882573.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNX27_001309"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNX27_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY7882574.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="JNX27_001310"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="JNX27_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNX27_001311"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNX27_001311"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7882575.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNX27_001312"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNX27_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7882576.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="JNX27_001313"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="JNX27_001313"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7882577.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="JNX27_001314"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="JNX27_001314"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7882578.1"
                     /translation="MRFDTVIMGSGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNKENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="JNX27_001315"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="JNX27_001315"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7882579.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="JNX27_001316"
     CDS             12630..13529
                     /locus_tag="JNX27_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7882580.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13548..13769
                     /locus_tag="JNX27_001317"
     CDS             13548..13769
                     /locus_tag="JNX27_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882581.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQQGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13810..14280)
                     /locus_tag="JNX27_001318"
     CDS             complement(13810..14280)
                     /locus_tag="JNX27_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7882582.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14335..15537)
                     /locus_tag="JNX27_001319"
     CDS             complement(14335..15537)
                     /locus_tag="JNX27_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY7882583.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="JNX27_001320"
     CDS             complement(15624..16166)
                     /locus_tag="JNX27_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882584.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="JNX27_001321"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="JNX27_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7882585.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /locus_tag="JNX27_001322"
     CDS             complement(16909..17511)
                     /locus_tag="JNX27_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7882586.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="JNX27_001323"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="JNX27_001323"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7882587.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="JNX27_001324"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="JNX27_001324"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7882588.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="JNX27_001325"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="JNX27_001325"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY7882589.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYDS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /locus_tag="JNX27_001326"
     CDS             21909..22799
                     /locus_tag="JNX27_001326"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7882590.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24444
                     /gene="arnT"
                     /locus_tag="JNX27_001327"
                     /pseudo
     CDS             22799..24444
                     /gene="arnT"
                     /locus_tag="JNX27_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24441..24776
                     /gene="arnE"
                     /locus_tag="JNX27_001328"
     CDS             24441..24776
                     /gene="arnE"
                     /locus_tag="JNX27_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7882591.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24776..25162
                     /gene="arnF"
                     /locus_tag="JNX27_001329"
     CDS             24776..25162
                     /gene="arnF"
                     /locus_tag="JNX27_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7882592.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="JNX27_001330"
     CDS             complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="JNX27_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7882593.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25533..26888)
                     /gene="menE"
                     /locus_tag="JNX27_001331"
     CDS             complement(25533..26888)
                     /gene="menE"
                     /locus_tag="JNX27_001331"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7882594.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26885..27847)
                     /gene="menC"
                     /locus_tag="JNX27_001332"
     CDS             complement(26885..27847)
                     /gene="menC"
                     /locus_tag="JNX27_001332"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7882595.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFTKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27847..28704)
                     /gene="menB"
                     /locus_tag="JNX27_001333"
     CDS             complement(27847..28704)
                     /gene="menB"
                     /locus_tag="JNX27_001333"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7882596.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDICYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28719..29477)
                     /gene="menH"
                     /locus_tag="JNX27_001334"
     CDS             complement(28719..29477)
                     /gene="menH"
                     /locus_tag="JNX27_001334"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7882597.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29474..31144)
                     /gene="menD"
                     /locus_tag="JNX27_001335"
     CDS             complement(29474..31144)
                     /gene="menD"
                     /locus_tag="JNX27_001335"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7882598.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31233..32528)
                     /gene="menF"
                     /locus_tag="JNX27_001336"
     CDS             complement(31233..32528)
                     /gene="menF"
                     /locus_tag="JNX27_001336"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7882599.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="JNX27_001337"
     CDS             complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="JNX27_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7882600.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32967..33428)
                     /locus_tag="JNX27_001338"
     CDS             complement(32967..33428)
                     /locus_tag="JNX27_001338"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7882601.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDCIPHIGMAREVIQA"
     gene            33493..34410
                     /gene="rbn"
                     /locus_tag="JNX27_001339"
     CDS             33493..34410
                     /gene="rbn"
                     /locus_tag="JNX27_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7882602.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34601..35272
                     /locus_tag="JNX27_001340"
                     /pseudo
     CDS             34601..35272
                     /locus_tag="JNX27_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8673 a         9421 c         8716 g         8462 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa gggatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgaggaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgtt ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcagc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa taaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgcagatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attacccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gagataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcagcaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatgattc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    23221 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    23281 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    23341 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    23401 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    23461 ctggcgatag cgcagcggga gcctgacttg tggcactatt ttttctgggt tgagcatatt
    23521 caacgctttg cactagatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    23581 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    23641 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    23701 tttttctccg tcgctaaagg caaattgccc acctatattc tttcttgctt tgcacctctg
    23761 gcaatgctgt tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    23821 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtgatc
    23881 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    23941 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    24001 gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    24061 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    24121 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    24181 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    24241 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    24301 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    24361 gatatcaaca gtctcgccat tccgcccgca gatgccatcg ttcgtcagga gcgtctggtg
    24421 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    24481 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    24541 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    24601 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    24661 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    24721 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    24781 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    24841 tgcggcaagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    24901 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    24961 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    25021 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    25081 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    25141 cacgacaaaa caacgttact gagttttccc tgccgctgta cactgccgtt cccactcatc
    25201 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    25261 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    25321 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    25381 atcttgttta ttgcagcacg attttttgtg ccagccattc cattgcatta tcctgtttgc
    25441 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
    25501 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
    25561 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
    25621 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
    25681 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggaa
    25741 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
    25801 tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg
    25861 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
    25921 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
    25981 ttattaacga ttttcacttc ccgacccggc agcggcaaac caacgtctgc caggccgtcg
    26041 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
    26101 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
    26161 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
    26221 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
    26281 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
    26341 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
    26401 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaacc ggtagaaccc
    26461 gaggtcaacg tcattgagca cagacgcgtc ggctgccacg cagcggcatg tacgccttca
    26521 accagctgaa tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc
    26581 gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg cagctgaggg
    26641 ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
    26701 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
    26761 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
    26821 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
    26881 aagatcataa caactgctcc agtacatcag cgtccactaa cggcagtggg ctccccggcc
    26941 agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg
    27001 gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc taagctcgac tcaatggaag
    27061 aactgatcac cgccgtcagc cccagcgcat gcgccgcctg tacctgctcg cgtacttttt
    27121 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    27181 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    27241 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    27301 gataatccgg gttaacatat ttggtaaact gctgaccttt cagcggtgtc caggcgcgat
    27361 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagattc accaccatgc
    27421 cgtcgcgcac cgcttcgtac aatcccactt tgaccttcgc cactttctcg cccggcatat
    27481 cggcaagttt gaggatcaga tcgtccggat cgccattaca cagcggtgcc gcacggtagt
    27541 tggctgcttg cggcaacgta tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca
    27601 cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    27661 gcacgctttg cgcctcttcc cagttttcct ggctgaagcc cggcagtggg gagatctccc
    27721 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    27781 gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    27841 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    27901 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    27961 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag
    28021 cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt tttccagatc
    28081 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg cgtcgtactg
    28141 acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag ccatgtagga
    28201 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    28261 atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc cgatggagta
    28321 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    28381 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    28441 ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga tcacgccgat
    28501 attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    28561 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt cggtggattt
    28621 ttcataacaa atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaagg
    28681 cattgcttca tcaggataaa tcatattgtg tcctttagtc aaaaacgcaa gatctgcgcc
    28741 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    28801 acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca
    28861 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    28921 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    28981 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    29041 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    29101 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgataata
    29161 accccacaaa gccccgccaa cccctggcaa gccgccatca tcgccacccg tccgccaagc
    29221 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    29281 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    29341 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    29401 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    29461 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    29521 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    29581 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    29641 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    29701 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    29761 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    29821 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    29881 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    29941 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat tgcccctgtt
    30001 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg
    30061 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    30121 gtttttctgc cggatgcagc tccagccagt cggcaatatt gccaattaag cgacgtccgc
    30181 gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac tcttctggtt
    30241 cacagcttgc ctgccattgc aggagccgtt tccccgtcag gctgcttccc agttgcacca
    30301 caatttgtgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    30361 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    30421 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    30481 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga cgaggcgctt
    30541 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    30601 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    30661 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    30721 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    30781 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    30841 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    30901 ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    30961 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    31021 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    31081 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    31141 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    31201 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcagc
    31261 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    31321 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    31381 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    31441 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    31501 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    31561 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    31621 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    31681 cgctggtttt tatcatccgc catcagcctc tctcctaact gctgcgcctg cttatcatca
    31741 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    31801 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    31861 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    31921 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc acctttggcg
    31981 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    32041 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    32101 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcaaccgc
    32161 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    32221 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    32281 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    32341 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    32401 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    32461 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    32521 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    32581 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    32641 ccgacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta gtcatcagca
    32701 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct aacccgtttt
    32761 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcggcg
    32821 ggctcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt caggtcgtca
    32881 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    32941 cactaactat agacaagagt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    33001 tgtgggatac aatcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    33061 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgta
    33121 catgtttcga gtgttttact catcagctgc tgccccactt tttcaccacg caacgcttca
    33181 ctgacaatca cccgacctat aacgaccggc tcaagatcat catcactttt cagaatcctc
    33241 gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    33301 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    33361 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    33421 tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata ttttttgaaa
    33481 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    33541 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    33601 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    33661 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    33721 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    33781 atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg
    33841 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    33901 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    33961 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    34021 gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    34081 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    34141 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    34201 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    34261 ctagcccgtg aggctggagt cggcaagcta atcattaccc acgtcagctc gcgctatgat
    34321 gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg
    34381 gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac agggtgaaac
    34441 ttgccctgtt atagcaaccc gcgctacata tatccaccca cacaggcata ttgagcgatt
    34501 tttgatattt attcatctcc ttacgcacta tattcagagc aataagttgc ctcttttcat
    34561 cttgattcag cacaaccttt gcaactaaaa ggagcccatg atggttacag ttgtcagcaa
    34621 ttattgtcaa ttatctcaaa cccaactcag tcagacattt gcagaaaaat ttactgtgac
    34681 cgaggaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag aaagtattga
    34741 gttactgcat aatattgcgt taggttatga tgaatttgga aaaaaagcag aagatattct
    34801 ttaccatatt gttagaaacc caacaaatga gacctatcga ttatccgact tattaaaaat
    34861 gcctgtttaa aattatataa tttggcacat accgcaacca actcccacct taaaccaact
    34921 ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc gaaattaatg
    34981 acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc aaaggtgctt
    35041 ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa aaatcgtcaa
    35101 ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc aaaaaatact
    35161 caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca cctggaagac
    35221 gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca gt
//
LOCUS       DADLXX010000027        35252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLXX010000027 DADLXX010000000
VERSION     DADLXX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.027"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNX27_001341"
     CDS             complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNX27_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY7882603.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1519..2649
                     /locus_tag="JNX27_001342"
     CDS             1519..2649
                     /locus_tag="JNX27_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7882604.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2649..3587
                     /gene="fruK"
                     /locus_tag="JNX27_001343"
     CDS             2649..3587
                     /gene="fruK"
                     /locus_tag="JNX27_001343"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7882605.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3604..5295
                     /gene="fruA"
                     /locus_tag="JNX27_001344"
     CDS             3604..5295
                     /gene="fruA"
                     /locus_tag="JNX27_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7882606.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5719..6660
                     /locus_tag="JNX27_001345"
     CDS             5719..6660
                     /locus_tag="JNX27_001345"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7882607.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6648..7586
                     /locus_tag="JNX27_001346"
     CDS             6648..7586
                     /locus_tag="JNX27_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY7882608.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7680..8930
                     /locus_tag="JNX27_001347"
     CDS             7680..8930
                     /locus_tag="JNX27_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7882609.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9002..9700)
                     /gene="yeiL"
                     /locus_tag="JNX27_001348"
     CDS             complement(9002..9700)
                     /gene="yeiL"
                     /locus_tag="JNX27_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY7882610.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLT
                     LEMDPENKFSGMMQ"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="JNX27_001349"
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="JNX27_001349"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7882611.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10872..12122
                     /locus_tag="JNX27_001350"
     CDS             10872..12122
                     /locus_tag="JNX27_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7882612.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVIGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13262)
                     /locus_tag="JNX27_001351"
                     /pseudo
     CDS             complement(12178..13262)
                     /locus_tag="JNX27_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="JNX27_001352"
     CDS             complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="JNX27_001352"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7882613.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14196..15245)
                     /locus_tag="JNX27_001353"
     CDS             complement(14196..15245)
                     /locus_tag="JNX27_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7882614.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15344..16225
                     /locus_tag="JNX27_001354"
     CDS             15344..16225
                     /locus_tag="JNX27_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7882615.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16431..17900
                     /gene="lysP"
                     /locus_tag="JNX27_001355"
     CDS             16431..17900
                     /gene="lysP"
                     /locus_tag="JNX27_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7882616.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18192..20183
                     /gene="cirA"
                     /locus_tag="JNX27_001356"
     CDS             18192..20183
                     /gene="cirA"
                     /locus_tag="JNX27_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY7882617.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20215..21051)
                     /gene="yeiG"
                     /locus_tag="JNX27_001357"
     CDS             complement(20215..21051)
                     /gene="yeiG"
                     /locus_tag="JNX27_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAY7882618.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21309..21977
                     /gene="folE"
                     /locus_tag="JNX27_001358"
     CDS             21309..21977
                     /gene="folE"
                     /locus_tag="JNX27_001358"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7882619.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21994..23151
                     /locus_tag="JNX27_001359"
     CDS             21994..23151
                     /locus_tag="JNX27_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7882620.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23293..24261
                     /gene="galS"
                     /locus_tag="JNX27_001360"
     CDS             23293..24261
                     /gene="galS"
                     /locus_tag="JNX27_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7882621.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNTNAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRAATVLCRR"
     gene            24612..25610
                     /gene="mglB"
                     /locus_tag="JNX27_001361"
     CDS             24612..25610
                     /gene="mglB"
                     /locus_tag="JNX27_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY7882622.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25671..27191
                     /gene="mglA"
                     /locus_tag="JNX27_001362"
     CDS             25671..27191
                     /gene="mglA"
                     /locus_tag="JNX27_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7882623.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27207..28217
                     /gene="mglC"
                     /locus_tag="JNX27_001363"
     CDS             27207..28217
                     /gene="mglC"
                     /locus_tag="JNX27_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7882624.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28253..29488)
                     /gene="preA"
                     /locus_tag="JNX27_001364"
     CDS             complement(28253..29488)
                     /gene="preA"
                     /locus_tag="JNX27_001364"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY7882625.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29482..30721)
                     /locus_tag="JNX27_001365"
                     /pseudo
     CDS             complement(29482..30721)
                     /locus_tag="JNX27_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(30915..31154)
                     /locus_tag="JNX27_001366"
     CDS             complement(30915..31154)
                     /locus_tag="JNX27_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7882626.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31157..31876)
                     /gene="sanA"
                     /locus_tag="JNX27_001367"
     CDS             complement(31157..31876)
                     /gene="sanA"
                     /locus_tag="JNX27_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7882627.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32026..32910)
                     /gene="cdd"
                     /locus_tag="JNX27_001368"
     CDS             complement(32026..32910)
                     /gene="cdd"
                     /locus_tag="JNX27_001368"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7882628.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33040..33735)
                     /locus_tag="JNX27_001369"
     CDS             complement(33040..33735)
                     /locus_tag="JNX27_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7882629.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEITASILPKSVTTPIAMSVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33732..34130)
                     /locus_tag="JNX27_001370"
     CDS             complement(33732..34130)
                     /locus_tag="JNX27_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7882630.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34369..>35252
                     /gene="dusC"
                     /locus_tag="JNX27_001371"
     CDS             34369..>35252
                     /gene="dusC"
                     /locus_tag="JNX27_001371"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY7882631.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8662 a         8614 c         9306 g         8670 t
ORIGIN      
        1 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
       61 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      121 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      181 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      241 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      301 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      361 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
      421 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      481 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      541 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      601 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      661 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      721 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      781 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      841 ttgcgattcc aggcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      901 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
      961 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1021 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1081 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1141 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1201 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1261 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1321 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1381 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1441 gtctttaacc ctaagccagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1501 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1561 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1621 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1681 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1741 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1801 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1861 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1921 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     1981 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2041 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2101 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2161 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2221 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2281 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2341 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     2401 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2461 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2521 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2581 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2641 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2701 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2761 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2821 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2881 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     2941 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt tgaagtcacc cccgccgact
     3001 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3061 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3121 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3181 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3241 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3301 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3361 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3421 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3481 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3541 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3601 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3661 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3721 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3781 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3841 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3901 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     3961 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4021 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4081 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4141 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4201 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4261 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4321 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4381 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4441 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4501 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4561 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4621 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4681 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4741 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4801 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4861 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4921 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     4981 caggaaggtg gcaaagccgc tctggtattg gggctgtgct tcatttcgga aggtgcaatt
     5041 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5101 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5161 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5221 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5281 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5341 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5401 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5461 tattttctgc ccttttttag tgttcctgtt ctgtgtatcc cgtcacaaat tcctcgcttt
     5521 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5581 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5641 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5701 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5761 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5821 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     5881 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     5941 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6001 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6061 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6121 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6181 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg
     6241 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6301 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6361 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6421 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6481 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6541 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6601 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
     6661 aaatttcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6721 aaccggttgt ggcgctggaa tcaaccatta tttctcacgg gatgccgttc ccacaaaatg
     6781 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6841 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6901 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     6961 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7021 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7081 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7141 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7201 taattggtta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7261 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7321 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7381 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7441 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
     7501 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7561 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7621 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7681 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7741 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7801 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7861 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7921 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     7981 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8041 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8101 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8161 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8221 tggcgtccat tgctggttcg atgatgattg gttatgccgg aatgggcgta ccaattgact
     8281 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8341 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8401 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8461 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8521 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8581 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8641 gcctgattgg gcagaaactg gcgattaacg aattcatcgc ttacctgagt ttctccccat
     8701 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8761 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8821 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8881 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctacgatt
     8941 tgtagaccgg ataaaacgta ttaagcgttg catccggcaa tcgcgctatt gcaaataatt
     9001 tttactgcat catcccggag aatttattct ccgggtccat ctccagcgtc agtcctgaca
     9061 actgctttct gtttttaatt agatatcctt tcttgctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcattaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcagca aagaaatcga
     9481 tcagcgacac gcgaccatta gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcatcagcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ccaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatagggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcgtg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacagttgc tgaacacctt gctgatgcaa tgcattcact
    12481 gcggcattac ggtcagcatc gctggtgatc gcctgtcccc ataaaatttc cagctccggt
    12541 aaagtgggtt tcagggtgtg aatatgcgcc agccagtgtt tgattttgcc cgctttgaat
    12601 tctgaaacgg tatcgacaaa caccgggatc tcatcagcga gggtaaagac ccattccagc
    12661 gcctcggctg tcaggttgca atcaaccagt accacgcccg catgacgaag taaatcgcgc
    12721 gacccgttca gtagttgcgg tgtcaactgt tccagcagat gggtatcgtt aatcgccagc
    12781 acggtttgat cgtctcgatt ggcaattgcc agatacgtcg atgtgctttg accatgcaaa
    12841 cgaacgcagc cggagacatt cacgccggca cggcgcgttt cttccaggag catttcgcca
    12901 taaaagtcat cgccaatcac tgaaagcaaa tggacgtcac ggcctaacag cgccagattg
    12961 tgggcgatat tgcgtccaac gccgcctgcc gaacaatgaa ttgtaccggg atgagaagcc
    13021 gcttgcgggt aatggatatc cgccatcccg cgaatatcca tattgattgt ccccaccact
    13081 acgcagtatt cctgctcggt gagaatgtaa cctttgcctt taatccggcc tttgcgcatt
    13141 aaatccataa tatgcgccgc aacgcgcgaa cgactgattt gcagcatgtc cgcaatttcg
    13201 ttctgctgaa tcagcgggtt acgccgtaaa attgcaagga tctccttttc ccggttattc
    13261 atcttcaggc taccgctttt tcagtttgtt gcgctttcag ccaggcgatc tcttctgccc
    13321 agatatccgg gttgatggtt tcaaggatca gcggaatgcc gtcgaaacgg tcgttctgca
    13381 taatccagcg gaacgcatca tgaccgatat tgccttcacc gaggctatga tggcggtcaa
    13441 cgcggctgcc aaaggtactt ttcgcatcgt taaggtgcat cccgcgcaga tacttaaagc
    13501 cgacaatacg ggcaaaatca gcgaacgttt tttcgcattc ggctggagta cgcaaatcat
    13561 acccggcagc gaaagcatgg caggtatcaa tgcagacgcc gacgcgggat ttatcttcca
    13621 cgccgtcgat aatcgccgcg agatgttcga atttaaaccc taagttactg ccctgaccgg
    13681 cggtgttttc aatcaccgct gttacacctt gagttttatc cagcgcaatg ttgatggatt
    13741 cggcaatacg cgcaaggcaa tcctcttctg aaatctgcat cagatggctg ccagggtgga
    13801 agttgagcaa agaaagcccc agttgttcgc aacgctgcat ttcatctata aaggcatcgc
    13861 gcgatttttc cagagcttca gtgaccggat gtccgaggtt gatcagataa ctgtcgtggg
    13921 gaagaatttg cgccgatgtg tagtgatatt tttcacaggc ggctttgaat tcatcgatgg
    13981 tttgcgtcgt gagcggtgcg gcacgccact gacgttggtt tttggtgaac aaggcaaacg
    14041 cggttgcgtc gatttcggcg gcgcgaattg cggcatttgc cagaccgcca gcagcactaa
    14101 cgtgcgctcc aatgtatttc atgcgaggac tcctgttaaa cccgctggag gaaaacggta
    14161 atgatagcgg gttaacagga gcaagaagta gtggtttatg cgatgacgct ttgaatcaca
    14221 tagttaatcg caccaccacc aacaatcagc caggcaaaca gtaccagtgc catcagcaga
    14281 ggtttcgccc cagctttttt cagcgcgctg acgtgagtgg tcagacccag cgccgccatc
    14341 gccattgcca gcaggaaggt atccagcgtt accagcatgt tcaccacgct ctgcggtaac
    14401 aggtggaacg agttaaagat ggcaactacg atgaacaaga tggcaaacca cggaatagtg
    14461 attttgcttt tctcgccgct gttcgcccca gacagctgtt taacacgcgc cgccagcagg
    14521 atgaggaacg gagccagcat catcacgcgc agcattttgg aaataactgc tgcgttttct
    14581 gcatccgggc tgatggcatg acctgccgcc accacctgcg ccacttcgtg cacagtagaa
    14641 ccaatgtaga taccgaaagt ttccggacta aaccattgag acatcagcgg atatatcgcc
    14701 gggtagagga aaatcgcgac ggtcccgaag ataacaacgg ttgcaacagc cacggttact
    14761 ttactggctt ccgctttcac taccggctca gtcgccagta ccgcggcagc accacagata
    14821 ctgctaccgg caccgatcaa ccaactggtg tgcttatcca gaccaaacac tttctgcccc
    14881 aggaagcaag ccagcaggaa ggtactggac agcgtcagca cgtcaatgat gatcccactg
    14941 ataccgacat cggcaatttg cgagaacgtc agacggaagc cataaagaat gatacccaga
    15001 cgtaataaat attgcttggc aaacagcaca ccaccgtcac agcttttcca gatgtgcgga
    15061 tagatggtgt tgcctaaaac catccccaac aagattgcga gggtgagggc actaaacccg
    15121 gcacccgcaa ccgcgggaat ggaaccaccc cacagggcga ccccggtgat aactgcactc
    15181 agggctaacc ccggaataaa atgccacagt gtacgatgtt gtttctgtaa ggtgatattc
    15241 gtcataaccc tctcctttaa ccgggcataa ggttacgact aactggttta aaaataaaat
    15301 tgattatata tttataatta atctttataa gtggtaagcg actatgcaca tcaccctccg
    15361 gcagttggaa gtttttgcag aagtattgaa aagtggatca accacccagg cgtcggtgat
    15421 gctggcgttg tcgcaatcag cagtgagcgc agccttgact gacctggaag ggcagcttgg
    15481 cgttcaactg tttgatcgcg tggggaaaag actggttgtt aatgaacacg ggcggctgct
    15541 ctatccgcga gcgttggcac tgcttgaaca ggcggttgaa atcgaacaac tgtttcgcga
    15601 agacaacggc gcgattcgta tctatgccag tagtaccatc ggtaactaca ttctgcctgc
    15661 agttatcgcc cgttatcgcc atgattatcc gcagttgccg attgaactta gcgttgggaa
    15721 cagccaggac gtgatgcaag cggtgctgga tttccgcgtt gatattggct ttattgaagg
    15781 accgtgccac agcactgaaa tcatttctga accgtggctg gaagacgagc tggtggtttt
    15841 cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc ttagaacagc tggccgctgc
    15901 gccgtggatc ctgcgtgaac gcggttccgg cacgcgggag attgtcgatt atctgttgct
    15961 gtcacattta ccgaagtttg agatggcgat ggaattaggt aactccgagg caatcaaaca
    16021 tgcggtgcgt catgggttgg gaattagttg cctgtcgcga cgtgtgattg aagatcaatt
    16081 gcaggcaggc acattaagtg aagttgcggt ccctctgccg cgcctgatgc gtacgttgtg
    16141 gcgtatacat catcggcaaa aacacctttc caacgcgcta cggcgctttc tggactattg
    16201 cgatcccgca aatgtgccgc gttaagttgc tgtacaagaa catgctggtg ctgtgtcgat
    16261 ttcgtgacgc agcgccttca gcatgcattc gccagaaaag agattggctg ctttacttat
    16321 aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga aggattgcca
    16381 atcgtctgct acaatcgcgc catcattttt aagatggata gcatttttgt atgggttccg
    16441 aaactaaaac tacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt cacctgacga
    16501 tgattgccat tggcggttcc atcggtacag gtctttttgt tgcctctggc gcaacgattt
    16561 ctcaggcagg tccgggcggg gcattgctct cgtatatgct gattggcctg atggtttact
    16621 tcctgatgac cagtctcggt gaattggctg catatatgcc ggtttccggt tcgtttgcca
    16681 cttacggtca gaactatgtt gaagaaggct ttggcttcgc gctgggctgg aactactggt
    16741 acaactgggc ggtgactatt gccgttgacc tggttgcagc acagctggtc atgagctggt
    16801 ggttcccgga tacaccgggc tggatctgga gtgcgttgtt cctcggcgtt atcttcctgt
    16861 tgaactacat ctctgttcgc ggctttggtg aagcggaata ctggttctca ctgatcaaag
    16921 tcacgacagt tattgtcttt atcatcgttg gcgtgctgat gattatcggt atcttcaaag
    16981 gcgcgcagcc tgcgggctgg agcaactgga caatcggcga agcgccgttt gctggtggtt
    17041 ttgcggcgat gatcggcgta gctatgattg tcggcttctc tttccaggga accgagctga
    17101 tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa cattccacgc gcggtacgtc
    17161 aggtattctg gcgaatcctg ttgttctatg tgttagcgat cctgattatc agcctgatta
    17221 ttccgtatac cgatccgagc ctgctgcgta acgatgttaa agacatcagc gtcagtccgt
    17281 tcaccctggt gttccagcac gcgggtctgc tctctgcggc ggcggtgatg aacgcagtta
    17341 ttctgacggc ggtgctgtca gcaggtaact ccggtatgta tgcgtctact cgtatgctgt
    17401 acaccctggc gtgtgacggt aaagcgccgc gcattttcgc taaattgtcg cgtggtggcg
    17461 tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg tttgtgcttc ctgacctcca
    17521 tgtttggcaa ccagacggta tacctgtggc tgctgaacac ctccgggatg acgggtttta
    17581 tcgcctggct ggggattgcc attagccact atcgcttccg tcgcggttac gtattgcagg
    17641 gacacgacat taacgatctg ccgtaccgtt caggtttctt cccactgggg ccgatcttcg
    17701 cattcattct gtgtctgatt atcactttgg gccagaacta cgaagcgttc ctgaaagata
    17761 ccattgactg gggcggcgta gcggcaacgt atattggtat cccgctgttc ctgattattt
    17821 ggttcggcta caagctgatt aaaggaactc acttcgtacg ctacagcgaa atgaagttcc
    17881 cgcagaacga taagaaataa gttttctccc ttccttgcta agccctctca accgagaggg
    17941 ctttttcaat tccatttccc tgacaaatca tgcggatata aaatttaaca tttggattga
    18001 taattgttat cgtttgcatt atcgttacga cgcaatcaaa aaaggctgac aaatcagagg
    18061 ctgttccggc tttctgggat gatcacctgc atacaaataa gtccaccgcg atgctgccgt
    18121 acgcaagggg acgtaaagaa gatgtgagcg atacccattt tattttcgta gttacctcat
    18181 ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt cgggctgtgt ttgtccgcta
    18241 tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg cgaaacgatg gttgtcactg
    18301 catcttccgt ggaacaaaat cttaaagatg cacccgccag tatcagcgtc attacccagg
    18361 aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt cctcaaagaa gtgcctggcg
    18421 tacaactgac gaacgaaggg gataaccgta agggcgttag tattcgtggt ctggacagca
    18481 gctacaccct gattcttgtc gacggtaaac gcgttaactc ccgcaatgcc gtcttccgcc
    18541 acaatgattt cgatctgaac tggatcccgg tcgattccat cgaacgtatt gaagtggtgc
    18601 gtggcccgat gtcgtcgcta tacggctccg atgcgctcgg cggtgtagtg aatatcatca
    18661 ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt cgataccacc attcaggaac
    18721 atcgcgatcg cggtgatacc tataacggtc agttctttac cagtggacca ttaattgatg
    18781 gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg tgaaaaggat gacccgcaaa
    18841 actcaacgac caccgatacc ggagaaacgc cgcgtattga aggattctcc agccgcgacg
    18901 gcaatgtcga atttgcctgg acaccgaatc aaaatcacga ttttactgcc ggatacggtt
    18961 tcgaccgtca ggatcgtgat tccgactcgc tggacaaaaa ccgcctggaa cgccagaact
    19021 actgcgtcag ccataatggg cgttgggatt acggcaccag cgaactgaaa tcctacggtg
    19081 agaaagtcga gaacaaaaac cctggcaaca gcagcccgat aacttccgaa agcaatacgg
    19141 tcgacggcaa atacacgttg ccgctgacgg cgattaatca gtttctcacg gttggcggtg
    19201 aatggcgtca cgacaaactt agcgatgcgg tgaacctgac cgggggaacc agctccaaaa
    19261 cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg gcggatcttc gagccgctgg
    19321 cgctgacgac cggcgtgcgt atggacgatc acgaaaccta cggtgaacac tggagtccgc
    19381 gtgcctacct ggtttataac gccaccgaca ccgtaacggt gaaagggggc tgggcgacgg
    19441 catttaaagc accttctctg ttgcaactta gccctgactg gacgagcaat tcctgccgtg
    19501 gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga aaccagcgaa agttgggagc
    19561 tggggcttta ctacatgggt gaagaaggct ggctggaagg gggtgaatcc agcgttatcg
    19621 ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg tacgtctgac gtcaacgctg
    19681 caccgggcta ccaaaacttt gttggttttg agacgggcgc taacggacgg cgcataccgg
    19741 tatttagcta ctacagcgtt aacaaagctc gtattcaggg cgtggaaacc gaactgaaaa
    19801 ttccgttcaa cgatgaatgg aaactgtcga tcaactacac ctacaacgat ggtcgtgatg
    19861 tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt ccatactgct aacggtacgc
    19921 tggactggaa accgctggcg ctggaagact ggtcattcta tgtttctggt cactataccg
    19981 ggcagaaacg cgccgacagc gcgacggcta aaacaccggg cggttatacc atctggaata
    20041 ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg cgcaggcgtg ctgaaccttg
    20101 gcgacaagga tctcagtcgt gacgactaca gctataacga agacggacgt cgttacttta
    20161 tggcagtgga ttatcgcttc tgatgagaag atgcccggcg aaccgggcgg actttcactt
    20221 cagtaaatac tgcgcatgga agcgcaggtg atcctctata aaagaggcga tgaagtagta
    20281 actgtgatca tatcccggct gaatacgcag cgtcatcggc cacgctttct ggcgcgcggc
    20341 ttctgccaat accgcaggtt gcaactggtc ggcaagaaac tgatcattat cgccctgatc
    20401 gataagcgtc gggatcgcat cctgcgcgtt actggcatac atcagcgcgc aactatccca
    20461 ttccagccat gcatttttgt cctcacctaa ataggtgcta aacgctttga ttccccacgg
    20521 gacgctgcac ggattcacaa tgggcgcaaa ggccgaaacg ctggtgtatt tacccggatt
    20581 tttcagcgcc ataatcagcg caccgtgacc acccattgag tgaccgctaa tggcgcagcg
    20641 gtcgctgaca ttaaattgcg actgaaccag cgccggtaat tcatcgcgca gataatcata
    20701 catccggtaa tacgtcgccc acggcggttg cgtggcatta agataaaagc ctgcgccctg
    20761 gcctaaatcg tagccatcgt cgttggcaac cttttcgccg cgcgggctgg tgtctggcat
    20821 caccagtaca atccccagtt ccgccgctac ccgctgggca cccgccttgg tggtgaagtt
    20881 ctcgtcattg caggttaatc cggaaagcca gtacagcact ggtggcgggg tgtgatcacg
    20941 agggggtggg agaaagatac tgaacgtcat cgggcagttt aaggtactgg agtcgtgtcg
    21001 ccagcgttgc tgccagcctt caaaacagcg gtgctcttcg agcatttcca tgcgtggctc
    21061 cttgttgtgt tgtttgcaat ttcttcatca taccgtttat tcaatgtgct gtgagtaact
    21121 ttcacttccg tatttgcata acgatgtttt aacatctgct gatgaaaggc agcggcaatt
    21181 acaataatta tcgctgtgaa tactggatta tgtgcgccgc ctcacgcaca ataatcaagc
    21241 tgtaaatcag cttaataact ttgcccccac gcagggcgga ggcgtcacac ctgcaggaga
    21301 aatcataaat gccatcactc agtaaagaag cggccctggt tcatgaagcg ttagttgcga
    21361 gaggactgga aacaccgctg cgcccgcccg tgcatgaaat ggataacgaa acgcgcaaaa
    21421 gccttattgc tggtcatatg accgaaatca tgcagctgct gaatctcgac ctagctgatg
    21481 acagtttgat ggaaacgccg catcgcatcg ctaaaatgta tgtcgatgaa attttctccg
    21541 gtctggatta cgccaatttc ccgaaaatca ccctcattga aaacaaaatg aaggtcgatg
    21601 aaatggtcac cgtgcgcgat atcactctga ccagtacctg tgaacaccat tttgttacca
    21661 tcgatggcaa agcgacggtg gcttatatcc cgaaagattc ggtgatcggt ctgtcaaaaa
    21721 ttaaccgtat cgtgcagttc tttgcccagc gtccgcaggt gcaggaacgt ctgacgcagc
    21781 aaattcttat tgcgctacaa acgctgctgg gcaccaataa cgtggctgtc tcgatcgacg
    21841 cggtgcatta ctgcgtgaag gcgcgtggca tccgcgatgc aaccagtgcc acgacaacga
    21901 cctctcttgg tggattgttc aaatccagtc agaatacgcg ccacgagttt ctgcgcgctg
    21961 tgcgccatca caactgatta aaaggcagga accatggagc gcaacgtcac gctcgatttt
    22021 gttcgcggcg tcgccattct ggggatcctg ctattaaaca tcagcgcctt tgggctacca
    22081 aaggcggctt atctcaatcc cgcctggtac gatgctatta cgccgcagga tgcatggacc
    22141 tgggcatttc tcgatctcat cggccaggtg aaattcctca cgctttttgc gctgctgttt
    22201 ggtgctggcc tgcaaatgtt gctgccccgt ggcagacgct ggatccagtc acggttaacg
    22261 ctgttagtct tgttgggctt tattcacggt ttattgttct gggacggcga tattctgctg
    22321 gcttacgggc tggtgggctt aatctgctgg tggctggtgc gcgatgcgcc atcggtaaaa
    22381 agcctgttta ataccggcgt catgctttat ctggtggggc ttggcgtttt gctgttattg
    22441 gggctgattt ccgacagcca gaccagccgc gcctggacgc cggatgcatc ggctattttg
    22501 tatgaaaaat actggaagct tcacggcggc gttgaagcga tcagtaatcg tgccgatggt
    22561 gttggcaaca gtttactggc actgggcgca cagtatggct ggcaactggc agggatgatg
    22621 ctcattggtg cggcgctgat gcgcagcggc tggctgaaag ggcagttcag cttacgtcac
    22681 tatcgtcgta ctggttttgt gctggtggcg attggggtga ccattaacct tcctgccatc
    22741 gccctgcaat ggcagctgga ctgggcgtat cgctggtgtg ccttcttact tcaaatgccg
    22801 cgggaactga gtgcgccgtt tcaggcgatt ggctatgcgt cgctgtttta tggcttctgg
    22861 ccgcaattga gccgctttaa gctggtgctt gcgatcgcct gcgtcggacg gatggcgctg
    22921 accaactatc tattgcaaac gctgatttgt accacgcttt tttaccacct cggtttgttt
    22981 atgcattttg accgcctgga gctgctggcg tttgttattc cggtatggct ggcgaatatc
    23041 ctcttctctg ttatctggct gcgttacttc cgccaggggc cggtggaatg gctctggcgt
    23101 cagttaactt tgcgtgctgc cggactggca atatctaaaa catcaagata acgatctgga
    23161 tcacattcgt taacaaaacg gctgtaaccg tttccattgc tgtgactcga ttcacgaagt
    23221 cctgtattca gtgctgacaa aatagccgcc agcaagcagt catttactgc aatctcataa
    23281 caggtagtga atatgatcac cattcgtgat gtagcgcgtc aggctggcgt ctctgtggca
    23341 acggtttccc gggtgctcaa taacagcacg ctggtcagtg ccgacacgcg tgaagcagta
    23401 atgaaagccg tgagtgagct ggattatcgg ccaaacacca atgcccaggc gctggcaact
    23461 caggttagcg acaccattgg cgtggtggtg atggacgttt ctgatgcgtt tttcggcgcg
    23521 ctggtaaaag cggtggatct ggtcgcccag cagcatcaga aatacgtgct aatcggcaat
    23581 agctatcatg aagcggaaaa agagcgtcac gccattgagg tgttaattcg ccagcgttgt
    23641 aatgcgttga ttgttcactc aaaagcattg agtgacgatg aactggcaca atttatggat
    23701 aacattcccg gtatggtgtt aatcaaccgc gttgtgccgg ggtacgccca tcgttgcgtt
    23761 tgcctagata atctcagcgg tgcccgaatg gcgacgcgca tgttgctgaa taacggtcat
    23821 caacgtattg gttatctttc ttccagccac ggcattgaag atgacgccat gcgtaaagca
    23881 ggctggatga gtgcgttgaa agagcaggat attattccgc cggaaagctg gattggcact
    23941 ggtacgccgg acatgccggg cggtgaggcg gcgatggttg aactgctggg gcgcaatcta
    24001 caacttaccg ctgtatttgc ttataacgac aatatggccg ctggcgcact gacagcatta
    24061 aaagataatg gcattgcgat tccgttacat ctctcaatca tcggtttcga tgatattccc
    24121 atcgcccgtt acaccgaccc gcaattaacg accgtgcgtt atcccattgc ttcaatggct
    24181 aaattagcca ccgaactggc cttgcagggg gcagcaggca atattgatcc tcgtgccgcc
    24241 actgttttat gccgacgtta gtgcgtcgcc attctgtcgc aacgcgccag aatgcggcgg
    24301 cgatcactaa ctcaacaaat caggcgatgt aaccgctttc aatctgtgag tgatttcaca
    24361 gtatcttaac aatgtgatag ctatgattgc accgttttaa tgttgtaacc cgtatgtaac
    24421 agtgaataat cactttcacc gaggtaatag cgtcataaca acaattaaag ccgttttctg
    24481 gagcgttacc gggcatggaa gaacgaattt taaaaagtga gcttcggcgt tcagtaacac
    24541 ttcattaact ctactgcccc gccgagcatt tatctcaagc actaccctgc ataagaaaaa
    24601 ccggagatac catgaataag aaggtgttaa ccctgtctgc tgtgatggcc agcatgttat
    24661 tcggtgccgc tgcacacgct gctgatactc gcattggtgt aacaatctat aagtacgacg
    24721 ataactttat gtctgtagtg cgcaaggcta ttgagcaaga tgcgaaagcc gcgccagatg
    24781 ttcagctgct gatgaatgat tctcagaatg accagtccaa gcagaacgat cagatcgacg
    24841 tattgctggc gaaaggggtg aaggcactgg caatcaacct ggttgacccg gcagctgcgg
    24901 gtacggtgat tgagaaagcg cgtgggcaaa acgtgccggt ggttttcttc aacaaagaac
    24961 cgtctcgtaa ggcgctggat agctacgaca aagcctacta cgttggcact gactccaaag
    25021 agtccggcat tattcagggc gatttgattg ctaaacactg ggcggcgaat cagggttggg
    25081 atctgaacaa agacggtcag attcagttcg tactgctgaa aggtgaaccg ggccatccgg
    25141 atgcagaagc acgtaccact tacgtgatta aagaattgaa cgataaaggc atcaaaactg
    25201 aacagttaca gttagatacc gcaatgtggg acaccgctca ggcgaaagat aagatggacg
    25261 cctggctgtc tggcccgaac gccaacaaaa tcgaagtggt tatcgccaac aacgatgcga
    25321 tggcaatggg cgcggtagaa gcactgaaag cacacaacaa gtccagcatt ccggtgtttg
    25381 gcgtcgatgc gctgccagaa gcgctggcgc tggtgaaatc cggtgcactg gcgggcacag
    25441 tactgaacga tgctaacaac caggcgaaag cgacctttga tctggcgaaa aacctggccg
    25501 atggcaaagg tgcggctgat ggcaccaact ggaaaatcga caacaaagtg gtccgcgtac
    25561 cttatgttgg cgtagataaa gacaacttgg ctgaattcag caagaaataa gactgattgg
    25621 gcgcagtcgt ttactgcgcc cttattgtat ggccaacaag gtaaaattat atggtcagct
    25681 caacgactcc gtcttccggg gaatacttgt tggaaatgag cggtatcaac aagtcctttc
    25741 ctggtgttaa ggcacttgat aacgttaatt taaaagtccg gccacattct atccatgcat
    25801 taatggggga aaacggtgca ggaaaatcga cattattaaa atgcctgttt ggtatttatc
    25861 aaaaagactc cggcaccatt ttattccagg gtaaagagat cgatttccat tccgccaaag
    25921 aggcactgga aaatggtatt tcgatggtac accaggagtt aaacctggta ttacaacgtt
    25981 cggtgatgga taacatgtgg ctggggcgat atcccaccaa aggcatgttt gtcgatcagg
    26041 acaaaatgta ccgcgaaacc aaagcgattt ttgatgaact ggatattgat atcgatccgc
    26101 gtgcgcgcgt cggcacatta tccgtttcgc aaatgcagat gatcgaaatc gccaaagcgt
    26161 tttcctataa cgcgaaaatt gtgattatgg atgaaccgac ttcttcgtta accgaaaaag
    26221 aggtcaatca tctgttcact attattcgta aattaaaaga gcgcggctgc ggtattgttt
    26281 atatctcgca taaaatggaa gaaatcttcc agttatgtga tgaagttacc gtattgcgcg
    26341 acggtcagtg gatcgccacc gaaccgctgg caggactgac gatggacaag atcatcggca
    26401 tgatggttgg gcgttctctt aatcagcgtt ttcctgacaa agaaaacaag ccgggcgaag
    26461 tcatcctcga ggtgcgtaac ctaacgtcac tgcgccagcc gtcgattcgc gatgtctcgt
    26521 ttgatctgca taaaggggag atcctcggta ttgccgggct ggtgggggcg aaacgtaccg
    26581 atattgttga gacgttattt ggtattcgcg agaaatcggc tggcaccatt acgttgcacg
    26641 gcaaacagat caataaccat aatgccaacg aagccataaa ccacggattt gcactggtaa
    26701 ctgaggagcg ccgctcaacg ggaatttatg cctatctgga tattggtttt aactcgttaa
    26761 tttccaatat tcgcaactac aaaaataaag ttggtttact ggataactcg cggatgaaaa
    26821 gcgataccca gtgggtgatt gattcgatgc gggtaaaaac gccgggtcat cggacgcaaa
    26881 ttggttcgct ctccggtggt aatcagcaaa aggtgattat tggtcgctgg ctactaacgc
    26941 aaccagaaat attaatgctc gatgaaccga cgcgcggtat tgatgtcggg gcgaagtttg
    27001 aaatttatca gttaattgcc gaactggcga agaaaggcaa ggggattatt attatctcct
    27061 ctgaaatgcc tgagttgtta gggataacag accgtattct ggtcatgagc aatggtctcg
    27121 tttccggaat tgtcgataca aaaacaacaa cgcaaaacga aattctgcgt cttgcgtctt
    27181 tgcaccttta agattagggg cttcccatga gtgcgttaaa taagaaaagt tttcttactt
    27241 acctgaaaga gggcggtatt tacgtcgttc ttttagtttt gctggcgatt attattttcc
    27301 aggacccaac atttttaagt ctgttgaact taagtaatat tctcacccag tcatcggtgc
    27361 gtattattat cgcgctcggt gtggcagggt taattgtcac ccaggggacc gatctttctg
    27421 ctggtcgtca ggtagggctg gcggcagtgg tggctgcgac attattgcag tccatggata
    27481 acgccaacaa agtgttcccg gaaatggcga cgatgccgat tgcgctggtt attctgattg
    27541 tctgtgccat tggtgcggtg atcggtttga tcaacggtct gattatcgct tatctcaacg
    27601 tgacgccgtt cattaccacg ctcggcacga tgatcatcgt ctatggcatc aactcgctct
    27661 attacgactt tgtcggggcg tcgccaattt ctggttttga cagtggcttc tctacctttg
    27721 ctcagggctt tgtcgcgctg gggagtttcc gtctctctta catcaccttc tacgcgttga
    27781 ttgcggtggc gttcgtctgg gtgttgtgga acaaaacccg cttcggtaag aacatttttg
    27841 ccattggcgg taacccggaa gcggcaaaag tatctggtgt caacgtcggc ctgaacctgc
    27901 tgatgatcta cgcgttgtct ggcgtgttct atgcctttgg cgggatgtta gaagccggac
    27961 gtatcggctc tgccaccaac aacctcggct ttatgtatga gctggatgct atcgcggcgt
    28021 gcgtggtagg cggtgtatcg ttcagcggcg gtgtggggac ggtgattggc gtggtgaccg
    28081 gggtaattat ttttaccgtc atcaactatg gcctgacgta tatcggcgta aacccatact
    28141 ggcagtacat catcaaaggg gcgattatta tcttcgccgt agcgctggat tcactgaaat
    28201 acgcgcgtaa gaagtgatgt gattgcggat attgccggat gcgtctctgg ttttacaacg
    28261 ttaccgggtg ttctttctcg cctttcttaa acttcacttc cccgagctca atacaaccca
    28321 ccgggcagac gtgaccacaa agcagacaac ccacacattt ctcggtatta caatgcggtg
    28381 tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac
    28441 agcgtccaca gccaacacat ttgtcaagat tgatacgggg atagacaata taactgcggt
    28501 ctaaatcttc cgccgggaca atattgttat tcgccagacc taccatctcc tgcaacgaat
    28561 caaatccctg atcggcgaga taatgcgata acccgctcgc catatcttct acgatgcgat
    28621 agccgtactg cataatgccg gtggtgacct gtaacgttgc tgcgccgagc aataaaaact
    28681 ccgccgcatc ttcccaggtt tcaatgccgc cgataccgct gattgggaaa tcgcgcagtt
    28741 ctggatgggt gcgcatttgc tgaatgaagc gcagggcgat cggttttacc gctttaccgg
    28801 aatatccgga aatactcgat ttaccgttaa cgatcggcat accgattttc tgattaagat
    28861 cgatattggt gatggattta acggtgttaa ttgccgcaat gccatctgcg ccgccgcgct
    28921 tcgccgccag cgccacttcg cacatatcac cgatattcgg cgtcatcttc gccagcattg
    28981 gcagcgtgga accccgtttc actgcccgac aatatttttt taccagctcc gggctttgcc
    29041 cgacatcgct acccatcgca tgagaagtca tttgcggaca ggagaagtta cactcgatca
    29101 tatccgcgcc agcttcttgc accaggcgcg ccagctcctc ccattgctgc tcattttccc
    29161 ccatgatcga agcgatcaat actttgtccg ggtaatcttc cttcagccga cgcagggcgg
    29221 tcagattctc ttccaacgga tgttcagcaa tctgctccat atttttgaag ccgataaaac
    29281 cggtatcttc tttcaccaga tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc
    29341 cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta ggctttggca cacatctcat
    29401 agcagttgcc taccggcgaa gaagagaggc agaacgggtt gggaaagttc acgccgcaaa
    29461 aagtaatcga aagatctttc gttaacatga gcaagctccc tctaatatag tgatgaatcg
    29521 cctcggcggc ttctttcccg gttttcacgg catagaccac ggttttgtca ccctcaacaa
    29581 tatcgccagc agcaaagact tgcggatcgc gggtctgata attttgtgtt ttgatggtgt
    29641 tacgctgctg ctctaactcc gcaaaggcat ccagtctggc atgctgaccg acggcgagaa
    29701 taattttatc tgccgccatc gtcagttcgc ctgataaccg tacatgctta aacgtgactt
    29761 tattgccttc cacggctact ggcgtgaatc catcgatgat cgaaacgccc agctcccggg
    29821 cgctggcaaa ctctttttcg cttgccggaa actcatctaa ctcttcacgt gctacgcaag
    29881 ttaccgcctg acagccgaga actttcagcg tgctggctac gtccatcgcg acatcaccgc
    29941 cgccgataat taatgcgctt tgtggaatgc tgatatcgcc ttgcgcctgt cgtacacgtt
    30001 gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa cagcggtagc ccggaaccgc
    30061 ttgataaccc cacggtgacc agtaccgcgc ggttttccgc tttcagttgc tccagggtga
    30121 gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc cattttttca atacgggcga
    30181 tctctgcatc cagcactgat tgcggtaacc ggaattgcgg aataccgtta cgcaaccagc
    30241 caccggggtg cgcttctttc tcataaatcg tgacgtcata accctggttt gtcagtgtca
    30301 cactggcctg caatccggca ggacctgcgc caataatcgc gactttgccg agcgttttag
    30361 taccgggctg ataaatttcc atcccggttt gttgttcaaa atcagtaaca aaacgctgta
    30421 agcggccgat atcaatggga gcatcaacac cggcacgggt acaaccgctt tggcataatt
    30481 tctccgtcgg gcacactctg gcacaaacgg caccgagggc attattttcg cgaattgtct
    30541 cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat aaatttcccc ggatcggtct
    30601 gtgccgggca agcctgacta cagggagcgt cgtgacataa taaacagcga gaggcttctt
    30661 taatcgccag taacggcgta aaagccggag tgagttcatc cagataattt tgttgcggca
    30721 tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc taaataaata ttgcgtgctg
    30781 atattaaggc tatgcgcgtg gggaaattta aactgtgaaa ggattcacaa atagctgcgg
    30841 ttcagaatgt tatatccgca gcttattgag tgaagtaata attattctca ataattaaca
    30901 gaaaagagaa aatattaacg atagaaaata agataaacaa tcatgccgat agataaaatt
    30961 ccgaagccaa tatatgccac catataagcg aagaagagtc cgggattttt tgctcgccag
    31021 acatacaccg gttccggtgc atttttaacc cgtttatcaa tcgcgcctgc gcgccagcct
    31081 ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa aaacggcaac gacaaaaaac
    31141 tgctgtacat ccataactac tttccttgtt tcttttgtaa ttcaagtaac tgttcgggtg
    31201 taacggcggg atacccctgc gcatcttccg gcacctggtg catagccgga atagggacca
    31261 gcggccctaa aaaacgcggt tcacgtttaa aaatataaag gtcagccagc gcaccgaaac
    31321 gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt cggtgacggt acggcataac
    31381 actgagcctg aatccccata tgcagcgcaa taaataatgc tcgctcacag tggaaacgtt
    31441 gggtgataat aatgaaatca ttagtatcga aaactttgcg tgtacgcacg atggaatcca
    31501 gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg gtcgacacca gcagcgatta
    31561 aatctttgcg catggtcatc ggctcgttat aactttgcaa tgcgttatcg ccgctcagta
    31621 atagataatt taccttaccg ctgttatagg cattaatcgc tccttgaatg cggtagcgat
    31681 aatactgatt aataacgccg gtacgataat attttgctgt gccgagcacc acgccgacct
    31741 ggcggtaggg gagatcctgc aattcgtcgt agatataagg cgcggttttc cagctcatcc
    31801 agcgatcgag gccgagcaca gtcaacagca gcaagccgat caggactaac aggctgagga
    31861 acacgcgctt taacatgcag gagactcgag ctaaggaaaa tttccatcag gctactttac
    31921 ccgctggttt tgcgcaagaa acggcaaact gtttgccggg aatttcgaca ccaggagagg
    31981 gctggcaagc cggacaaggc gttcacgccg catccggcac caggcttaag cgagaagcac
    32041 gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg gaggtggcat cccactgaat
    32101 caacggcgca tcggcttttt ctgccagaac cgcgcgctgg atatccgggt aatcataacc
    32161 cttgagattc aacagaatta acgccccctg caacggcggc agagtcgggt tgaatgcggc
    32221 gttttcagcg tagctgccgc tgaaaatacg accgtcttta cattccagcg cgacaccgct
    32281 tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct gcaatcgccg cctgagaaag
    32341 cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc atcagcagcg ttttaatctc
    32401 cagatctttc ggcccaaagg catccggcag atagtcacgc agcgcgtgtg cctcgcggcc
    32461 cggcaaatga atacgcagat ccagaccgct gttcagttca ttcataaact gacggcagtg
    32521 accacaaggc gtatagttaa cggtgatggc tgcaagcgct ttttcaccac tcaaccaggc
    32581 gtggctgatc gcgctttgtt cggcatgaac ggtttgctgc attgttgcac caataaattc
    32641 catattggca ccaaaatacc aggttccgct cacaccgcgc gcaattgcgc caacattaaa
    32701 attcgacaat ggcgtacgcg cacaggccgc cgccagcgga agtagtgcga atgccagcgc
    32761 gtcttcgtcc agccccgttg cgctcttcag cgatgagact tgctccccgg tcaacaaagc
    32821 ggggaagtac ttgtctgcca gaataggttc cagtgcagat tgcaagttat ccgcaagttg
    32881 ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct cataatcatg taatgggata
    32941 acagtttacg aacccgttat ggctttatat gtgatctgaa tctcattaat tatgcaacac
    33001 gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt tagcccatta ctgccagaat
    33061 aatcgggaaa aggaacggtg cgatcagcga agtaattatc ccgcataaca ccagcgctag
    33121 cgaactaaat gcaccttcct gataatccag ctcggcgcag cgcgccgtac cgagggcgtg
    33181 cgaggcagtc cccattgcca gaccgcgcgc agctttggta cgaatacgca tcgcattaag
    33241 caatgtatgg ccaaataccg cgccgaggat gccgacgaaa atcacgcaaa ctgcgctgat
    33301 tgccggaata ccgccaatac tgccgccaac cgacattgca ataggcgtgg tgactgattt
    33361 cggcaggatt gacgcggtga tttccggtga agcgcccatc aacaatgcca cggaagtccc
    33421 cgtcaccatc gcaaccacgc tgccgataaa acagatggtg atgatcgatt tccagcgcgc
    33481 gcggatctgg tgtagctgct catataaagg ataggccagc gcgacgaccg ccggttgcag
    33541 cagatcgttt agcacttcgc tacctttaaa gtaactgtcg taagagatgc cggtcagcat
    33601 taaaaaagga atgatcacca ccattgctac cagcaacggg ttaagcaggg gaaacttata
    33661 ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac gtcagcggta atgaccacca
    33721 gatattcgcc atcattcttc tgatcctttc tgacctacga ctttacgttc accgtgtacc
    33781 agttgtgaac tccagctcac caccagaaaa accaccagcg tactgacagc acaggaaacc
    33841 actaccgggc caaactgcgc gcggagcaaa tcaaaatatt gcatgacgcc tacgccaatc
    33901 ggcacaaaca atagcgccat atagcgaatc agtacgtagc accccggatt gacccatttc
    33961 gccggaagaa tttgcaaggc cagcaggaca aacaggatca gcatcccgat gatgctgccc
    34021 ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg catacaggca ggcataaatc
    34081 aggacgaaag cgcgtaaata ttgccagata atgttcagtg tcttactcat aatcaacacc
    34141 ccagatgaga tggattcatc atacaattaa ttcgtaaaac gtgctaccga tcacattaag
    34201 aaatcagcgc ttattttttc gctacccggt attacgcaat gagcagcgaa ctcaggcata
    34261 catcaagcat agagagggat tgcagtttgc agcaatccct ctttgcagac ctttaatccg
    34321 ctgaccatag gcgctaacat agcgcctcat ttttttgcgg gtgatgatat acgtgtgtta
    34381 ctggcaccga tggagggagt gcttgactct ctggtgcgtg aattgctgac cgaagttaac
    34441 gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg atcaactgct gccggtaaaa
    34501 gtctttcatc gcatttgccc tgagctacaa aacgccagcc ggacaccatc tggtacgctg
    34561 gtgcgcgtgc agttgttagg tcagttccca caatggctgg cagagaacgc cgcccgtgcg
    34621 gtggagttag gttcctgggg cgtggatctc aattgcggct gcccgtcgaa aacggttaac
    34681 ggtagcggcg gcggggcgac gttactcaaa gatcctgaac tcatctacca gggggcaaaa
    34741 gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg tgaaagtgcg tctgggctgg
    34801 gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc aacaggctgg cgctacggag
    34861 ctggtggtgc atgggcggac gaaagagcag ggttaccgcg cggagcatat tgactggcag
    34921 gcgattggcg agattcgcca gcggctgaat attccggtga ttgccaacgg tgaaatctgg
    34981 gactggcaga gcgcgcaaca atgcatggcg atcagcggct gcgacgcagt gatgattggt
    35041 cgcggggcgc tcaatattcc caacctgagc cgggtggtaa aatataacga accgcgaatg
    35101 ccgtggccgg aggtggttgc tttgctgcaa aaatataccc gtctggaaaa gcagggcgat
    35161 accgggttat atcacgttgc gcggattaaa cagtggttga gttatttgcg taaagaatac
    35221 gatgaagcaa cggaattatt tcagcatgtt cg
//
LOCUS       DADLXX010000028        34866 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLXX010000028 DADLXX010000000
VERSION     DADLXX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.028"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(18..93)
                     /locus_tag="JNX27_001372"
     tRNA            complement(18..93)
                     /locus_tag="JNX27_001372"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JNX27_001373"
     tRNA            complement(152..228)
                     /locus_tag="JNX27_001373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JNX27_001374"
     CDS             complement(331..1716)
                     /locus_tag="JNX27_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7882632.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JNX27_001375"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JNX27_001375"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7882633.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JNX27_001376"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JNX27_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7882634.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIFRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JNX27_001377"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JNX27_001377"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7882635.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTWMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JNX27_001378"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JNX27_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7882636.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /locus_tag="JNX27_001379"
     CDS             complement(6327..7457)
                     /locus_tag="JNX27_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY7882637.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JNX27_001380"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JNX27_001380"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY7882638.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8977)
                     /gene="rfbA"
                     /locus_tag="JNX27_001381"
     CDS             complement(8114..8977)
                     /gene="rfbA"
                     /locus_tag="JNX27_001381"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7882639.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIAWRNGWLDDEGVKRAASSLAKTGYGQYL
                     LELLRARPRQY"
     gene            complement(8996..10063)
                     /gene="rffG"
                     /locus_tag="JNX27_001382"
     CDS             complement(8996..10063)
                     /gene="rffG"
                     /locus_tag="JNX27_001382"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7882640.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10063..11325)
                     /gene="wecC"
                     /locus_tag="JNX27_001383"
     CDS             complement(10063..11325)
                     /gene="wecC"
                     /locus_tag="JNX27_001383"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7882641.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11322..12452)
                     /gene="wecB"
                     /locus_tag="JNX27_001384"
     CDS             complement(11322..12452)
                     /gene="wecB"
                     /locus_tag="JNX27_001384"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7882642.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12508..13554)
                     /gene="wzzE"
                     /locus_tag="JNX27_001385"
     CDS             complement(12508..13554)
                     /gene="wzzE"
                     /locus_tag="JNX27_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7882643.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13566..14669)
                     /gene="wecA"
                     /locus_tag="JNX27_001386"
     CDS             complement(13566..14669)
                     /gene="wecA"
                     /locus_tag="JNX27_001386"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7882644.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14909..16168)
                     /gene="rho"
                     /locus_tag="JNX27_001387"
     CDS             complement(14909..16168)
                     /gene="rho"
                     /locus_tag="JNX27_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7882645.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16253..16354)
                     /gene="rhoL"
                     /locus_tag="JNX27_001388"
     CDS             complement(16253..16354)
                     /gene="rhoL"
                     /locus_tag="JNX27_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7882646.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16495..16824)
                     /gene="trxA"
                     /locus_tag="JNX27_001389"
     CDS             complement(16495..16824)
                     /gene="trxA"
                     /locus_tag="JNX27_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7882647.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16955..18220
                     /gene="rhlB"
                     /locus_tag="JNX27_001390"
     CDS             16955..18220
                     /gene="rhlB"
                     /locus_tag="JNX27_001390"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7882648.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18354..19838
                     /gene="gppA"
                     /locus_tag="JNX27_001391"
     CDS             18354..19838
                     /gene="gppA"
                     /locus_tag="JNX27_001391"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7882649.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19885..21906)
                     /gene="rep"
                     /locus_tag="JNX27_001392"
     CDS             complement(19885..21906)
                     /gene="rep"
                     /locus_tag="JNX27_001392"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7882650.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            21993..22274
                     /gene="ppiC"
                     /locus_tag="JNX27_001393"
     CDS             21993..22274
                     /gene="ppiC"
                     /locus_tag="JNX27_001393"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7882651.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22320..23795)
                     /gene="ilvC"
                     /locus_tag="JNX27_001394"
     CDS             complement(22320..23795)
                     /gene="ilvC"
                     /locus_tag="JNX27_001394"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7882652.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23945..24835
                     /gene="ilvY"
                     /locus_tag="JNX27_001395"
     CDS             23945..24835
                     /gene="ilvY"
                     /locus_tag="JNX27_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7882653.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24832..26376)
                     /gene="ilvA"
                     /locus_tag="JNX27_001396"
     CDS             complement(24832..26376)
                     /gene="ilvA"
                     /locus_tag="JNX27_001396"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7882654.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26379..28229)
                     /gene="ilvD"
                     /locus_tag="JNX27_001397"
     CDS             complement(26379..28229)
                     /gene="ilvD"
                     /locus_tag="JNX27_001397"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7882655.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28294..29223)
                     /gene="ilvE"
                     /locus_tag="JNX27_001398"
     CDS             complement(28294..29223)
                     /gene="ilvE"
                     /locus_tag="JNX27_001398"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7882656.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29243..29506)
                     /gene="ilvM"
                     /locus_tag="JNX27_001399"
     CDS             complement(29243..29506)
                     /gene="ilvM"
                     /locus_tag="JNX27_001399"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7882657.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29503..31149)
                     /locus_tag="JNX27_001400"
     CDS             complement(29503..31149)
                     /locus_tag="JNX27_001400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7882658.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31152..31202)
                     /locus_tag="JNX27_001401"
     CDS             complement(31152..31202)
                     /locus_tag="JNX27_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882659.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31288..31386)
                     /gene="ilvL"
                     /locus_tag="JNX27_001402"
     CDS             complement(31288..31386)
                     /gene="ilvL"
                     /locus_tag="JNX27_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7882660.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31738..33258
                     /locus_tag="JNX27_001403"
     CDS             31738..33258
                     /locus_tag="JNX27_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY7882661.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33283..33621)
                     /gene="maoP"
                     /locus_tag="JNX27_001404"
     CDS             complement(33283..33621)
                     /gene="maoP"
                     /locus_tag="JNX27_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7882662.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33740..34579
                     /gene="hdfR"
                     /locus_tag="JNX27_001405"
     CDS             33740..34579
                     /gene="hdfR"
                     /locus_tag="JNX27_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7882663.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLSGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGSLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34675..34750)
                     /locus_tag="JNX27_001406"
     tRNA            complement(34675..34750)
                     /locus_tag="JNX27_001406"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34715..34717),aa:Trp,seq:cca)
     gene            complement(34759..34835)
                     /locus_tag="JNX27_001407"
     tRNA            complement(34759..34835)
                     /locus_tag="JNX27_001407"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34799..34801),aa:Asp,seq:gtc)
BASE COUNT         8502 a         9542 c         8805 g         8017 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt atgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gaaaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccacgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcacgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcataa
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctta aagccctggc gtttttctac tgtctgcaca aaagtgctgg
     8281 cctcaatcag gctgtcgtga gtgtcggtat ccagccaggc aaatccgcgc ccgagcagtt
     8341 caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac
     8401 cgcgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa
     8461 gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttcaagc gagatagcgc
     8521 ggaaattgtc gtcaaattcc accacgccaa agcgttccgg gtccatcacc tgatagtcaa
     8581 aaaccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg aagccctgac
     8641 cgaagaagat gttatcgccc agcaccagac aagaaggttc accattaagg aaggtttcac
     8701 cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcatat tccagctgga
     8761 taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag
     8821 tgatgatgag aatttcgcgg ataccggcca gcatcagcac cgacagcgga tagtaaatca
     8881 ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc
     8941 gggtaccgga accgcccgcc aggataatac ccttcatgtt tgcctccgct gaaaattagc
     9001 ctttcagacc taaacgctca ccctgatagc tgccgtcctg cacctgcttc caccagcttt
     9061 cattagccag ataccactga accgttttac gcattccact ttcaaaggtt tcctgcggca
     9121 gccagccaag ttcacgggca attttcgaag catcaatggc ataacgcaga tcatgccccg
     9181 gacggtcagc gacaaaggtg atcaggtcac gatagtgcgc cacgccgtgc ggcttgttcg
     9241 aagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt
     9301 tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg
     9361 cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg
     9421 gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt
     9481 ggtaatgacc gtagttattc gagcagttgg tgatcagcgt cggcagaccg taggtccgca
     9541 gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg
     9601 catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt
     9661 cggtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc gtattccagt
     9721 aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca ataaacgctg
     9781 ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg
     9841 gttgatgctc agtgaatacg cgtgccagtt ctgcccgatc gcagatatca actttctcaa
     9901 aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg gcgtaggtca
     9961 gcttatcgac cactaccacc gcatcgctcg tttcgttgat gatataacgc accagcgccg
    10021 agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc agactccttt
    10081 ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttga actgactatg
    10141 atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt
    10201 acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagcgtttc
    10261 gccgctgtgc cactgggcga tcagttcagc gatttccatc gccgggcttt cgcgcaggtc
    10321 atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt
    10381 atcggtagca gcaaggcagt cggcaaccgc cgctttcacc tgatcgataa cccagaacgg
    10441 tttgtgatcg ttaacttcgc gcgcggtacg gataagccgc gcctgctggg ggttctgtgc
    10501 cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccagggc caggctgaag
    10561 aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt taatcccctg
    10621 atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc ggaagctgtt
    10681 ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag
    10741 gaaaattttg tacaattcgc tggcgcgggc cgaacaaacc ggcgtcatac caccaatcac
    10801 gcgatcgttt ttaatcagct cgaccataac ctggcccggt aatacacgtt ccgggcagta
    10861 agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtcagat ccgggcgcat
    10921 ctctgctaac cattcagcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat
    10981 caccagggcg ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt
    11041 catatctggc tcatgatcgc ctttaaacgg cgtaggtaca gcaatcagcc aggcatccgc
    11101 ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct
    11161 cgccaaatca ggctcgacga tatggatttc gccacgattg atggtatcaa ccgcgtgttg
    11221 gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag
    11281 cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta
    11341 ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg
    11401 cggctcatag cttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt
    11461 cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca
    11521 gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg
    11581 cctgagtcag tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc
    11641 tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac cggctctctg
    11701 acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt ggtggcgatg
    11761 tctgccagcg cgtggcagat ttcttcaaag ccacgaccga aactctcacg cctatgaccg
    11821 gtcaccagaa tcatcttttt atcgggggcg ataaacgggt aatttgccgc cagttctgaa
    11881 cgcagcgtgt cgctgctcat cacctggtca cgcacccata acagtgcatc aatgactgta
    11941 ttaccggtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt ttgccgggaa
    12001 gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg gttagcctct
    12061 tccggccacg gcgaatagag atcgcccgtg cgcagaccag cctcaacgtg accaacagga
    12121 atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgtcgtatc gccgtgaacc
    12181 agcacaacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag
    12241 gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg
    12301 gaaaagagtt tcagtacctg atcaagcatc tcccgatgct gcgcagtgac gcaaacttta
    12361 gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gagccatctt gatggcttcc
    12421 gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc
    12481 gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc
    12541 agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac gacgtgggct
    12601 atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa aacgcggatc
    12661 cagggttgga ccaacattca gggtgtttaa catggctcga ttctgatcat agtcgagatc
    12721 aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag
    12781 caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat
    12841 attatgctgc tcagcaattt tcagcgcctg ctcaatgctg ttcatccggc ggtcgtagat
    12901 ggctttcgcc acctcttcct gacgcttcac ctgagctttc atctggatgg tgcgtgccgc
    12961 ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg caaaagcaac
    13021 atactgacgt aacaggttat tagcgtcagg cgcggtttcg gcaataagct tcacgctgtc
    13081 attgaccgcg cgagtaaagt ctccggggat aaactggatg ttgttaatca tttcatccag
    13141 caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt cggtttgcag
    13201 ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt tataggcttc
    13261 gtccatgacc gatggttgat cggcagaagc catgtttgaa cggacatcca ggttacgcaa
    13321 aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat cggtaatcgc
    13381 cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta acgcaaacgc
    13441 cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca acccacgaat
    13501 atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc
    13561 ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg
    13621 cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata gaggaagaat
    13681 gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc
    13741 acgccaatgg aagcgagcag tgctgcggca agggtaatca gcacaaacgc ctgacgggaa
    13801 gtaaacccgg cacgcatgat caaatggtga atatgctggc ggtcaggaga gaatgggctc
    13861 atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt
    13921 atccacaaag cggtaaccgg gctgatggga tgggttttgc cctgggtcgt ttcgagcagg
    13981 atccagataa cggtaaaacc aatcagcgta ctgcccgcat cacccataaa gactttgtag
    14041 cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca
    14101 aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc gattgctgca
    14161 aacgagacgc aggacaaccc gcccagcaag ccatcaatgc catcaaccat gttgaacgca
    14221 ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc
    14281 tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa caccatcata
    14341 acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc aaaacgatca
    14401 tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga tgcatgcgga
    14461 atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca
    14521 acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac taaaccgact
    14581 ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg
    14641 agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag
    14701 aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc caccagccct
    14761 gtgcaaaaat taaccatttg atatgcaaat tgcataacat gccagcaaat tccaggaatt
    14821 ttcctaacat cgccagcgcg acattaagat tacagatata aaagcaaaac gccacgtaaa
    14881 cacgtggcgt ttttggcata agaccaattt atgagcgttt catcatttcg aagaaatcgt
    14941 cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg atttcgccca
    15001 tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga gtcgtgagca
    15061 gctcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg cgtttttcag
    15121 cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtaaataa
    15181 cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatggtc aggctgccgc
    15241 cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgaagg gcattggcgt
    15301 ccacaccacc ggtcaacact ttacctgacg ccggaacaac agtgttgtaa gcgcgcgcca
    15361 gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc aggcgtttgg
    15421 ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag
    15481 aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt
    15541 cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc tgagcaatgt
    15601 tctgcagcag catagtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac
    15661 gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta gaaccgttac
    15721 cacgttccat acgcagacga gagtttgcgt gcagcggggt taagttctca aagaggattt
    15781 tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat
    15841 agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt
    15901 tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc
    15961 tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa
    16021 agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac
    16081 gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg
    16141 gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc
    16201 tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac
    16261 tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat
    16321 tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa
    16381 attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg
    16441 actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc
    16501 caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca ctttggttgc
    16561 cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg
    16621 cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc
    16681 gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca
    16741 gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt
    16801 caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt
    16861 tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc
    16921 gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca
    16981 gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt
    17041 tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt
    17101 agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca
    17161 ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat
    17221 tatggcaccg acgcgtgaac ttgccgtgca gatccacgcc gacgcggaac cgctggcgga
    17281 agctaccggc ctgaagctgg gcctggctta cggtggtgat ggctatgaca aacagctgaa
    17341 agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa ttgactacgc
    17401 caagcagaac cacattaacc tcggtgccat tcaggtagtg gtactggacg aagccgatcg
    17461 catgtacgat ctaggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc
    17521 aaatcagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc
    17581 gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca
    17641 ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac
    17701 gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca aacaccgttg
    17761 tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga
    17821 tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg atctggatat
    17881 tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt
    17941 taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg
    18001 cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat tgaatttgcc
    18061 tgctattgag acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt
    18121 gatgaccgat ctgccaaaac cgctgcgcct cacgcgcccg cgcacaggca atggtccgcg
    18181 tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa
    18241 tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcttac tgtagtaagt
    18301 gattcgggtg aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt
    18361 ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg
    18421 tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat taaacgcaaa gtgcgtctgg
    18481 ctgctggcct gaatagcgaa aatgacctct ctaatgaagc aatggagcgc ggttggcaat
    18541 gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg
    18601 ctacggcgac attacgactt gccgtcaatg cgggtgattt tattgccaaa gcgcaggaaa
    18661 tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcgcgtctg atttatcagg
    18721 gcgttgctca caccactggt ggtgccgatc agcgcctggt ggtggatata ggcggtgcca
    18781 gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg
    18841 gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcaa gaaaattttg
    18901 atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc
    18961 acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga
    19021 tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg aaacagcgag
    19081 ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg gaacgtgcgt
    19141 tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg aatattcagt
    19201 gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg
    19261 ccgtcgagca ggatattcgc agccgtacac tgcgtaatat tcaacgccgc tttatgatcg
    19321 atattgatca ggcacagcgc gtagccaaag ttgcggctaa cttcttcgat caggtggaaa
    19381 acgaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg
    19441 aaatcggcct gagcgttgac ttcaaacaag ctccgcaaca cgctgcttat ctggtgcgta
    19501 acctggatct tcccggtttt acccccgcac agaagaaact gctggcgacg ctactgctca
    19561 accagactaa cccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccaccgcgcg
    19621 tcgcagaaca actctgccgt ttactccgcc tggctatcat ttttgccagc cgtcgccgtg
    19681 acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc
    19741 cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag gaaaaccagt
    19801 ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg
    19861 ctgacgcatc ttctccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag
    19921 attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcactgacca ctttgcgctc
    19981 ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg gctccgggcg
    20041 caccagttcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc
    20101 gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatact
    20161 gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag gaaactccag
    20221 cccttttgac gcatggagag tcatcagttg cacctgatcc agctcttctt cactctcacc
    20281 acgctccatc atgtcgcgta aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc
    20341 atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt
    20401 catgcgcatt tcggcagctt tcgggctggg cgatgtttcg tacagccagg attcataatc
    20461 catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat
    20521 ttctgccaac cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg
    20581 actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccactc
    20641 acccagcttt ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa cgatacgcag
    20701 aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat
    20761 ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat
    20821 caggaatttt tcaaacaccc gcgactgatg gttaccgcga taaagaatag cgtaatcttt
    20881 gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc gctcggcttc
    20941 atgttcttcg ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca gttcggagaa
    21001 cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat
    21061 acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg
    21121 gctcagcagc accaggtttt gcggacgtgc gccgcgccag gagtagatcg actggtcatc
    21181 gtcacccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg
    21241 gctggtgttg gtgtcctgat attcgtccac cagcaggtag cgaattttgt tctgccagcg
    21301 ctcgcggact tcttcattgc gttgcagaag tagcgtcggc aataaaatca gatcatcgaa
    21361 gtcgagcaca ttacaggctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat
    21421 acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga gatcgttctt
    21481 ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt caatcagccc
    21541 ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg aaaagttcgc
    21601 cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg tgtggaaggt
    21661 tgagatcatc agcccacgcg cctctttgcg acccagcgtc tgtccgacac gctctttcat
    21721 ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct gataaccgca
    21781 cccgcggatc agatgggcga ttttattggt gataacacga gttttaccgg aacccgcacc
    21841 cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag
    21901 acgcatagga aaatcgctca atgaaagtcg ggaggggaaa ataacagcgt ggtagtatag
    21961 ccagcctcat ccttaccact caaggcacga tcatggcaaa aacagcagca gcactgcata
    22021 tccttgtaaa agaagagaaa ctggctctgg atcttctcgt gcagattaag aacggtgccg
    22081 atttcggcaa gctggcgaag aaacactcca tttgcccatc aggcaaacgc ggcggtgatt
    22141 taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagttgtg ttctcttgtc
    22201 cggtactgga gccaaccggc ccgctgcaca cccagttcgg ttaccacatc attaaggtgc
    22261 tgtaccgtaa ctaatagcaa ggccttctcc gggagaaggc cttgaactct gaatcgctat
    22321 taacccgcaa cagcaatacg tttcatatcc gtcatatagc cgcgcagttt cttacctact
    22381 tgctcaatcg catggctgcg aatcgcttca tttacatcac gcagctgcgc gttatctacc
    22441 gcaccttccg gaatagcttt acccaaatcg cccggttgca gttctgccat aaacggtttc
    22501 agcagcggca cacaagcgta agagaacaga tagttaccgt actcggcagt atcagagata
    22561 accacgttca tttcatacag acgcttacgg gcgatggtgt tggcaatcag cggcagttcg
    22621 tgcagtgatt catagtaagc agattcttcg atgatgccgg aatcaaccat ggtttcgaac
    22681 gccagttcaa cgcccgcttt caccatcgcg atcatcagta cgcctttatc gaagtactcc
    22741 tgctcgccga ttttgccttc atactgcggc gcggtttcga aggcggtttt gccggtctct
    22801 tcacgccagg tcagcagttt cttatcatcg ttggcccagt ccaccatcat accggaggag
    22861 aattctccgg agatgatgtc gtccatatgt ttctggaaca gcggtgccat gatctctttc
    22921 agctgttcag acaacgcgta agcacgcagt ttcgccgggt tagagagacg gtccatcatc
    22981 agtgtgatac cgccctgctt cagcgcttcg gtgatggttt cccaaccgaa ctgaattagt
    23041 ttttctgcgt atgccggatc ggtgccctct tccaccagct tgtcgaagca cagcagcgag
    23101 ccagcctgca acataccgca caggatggtt tgctcaccca tcaggtcaga tttcacttcc
    23161 gcaacgaagg acgattccag cacgcccgca cggtgaccac cggttgctgc cgcccatgct
    23221 ttagcgatcg ccatgccttc gcctttcgga tcgttttccg ggtgaacggc aatcagcgtc
    23281 ggtacgccga atccacgttt gtactcttca cgcacttcgg tgccagggca tttcggcgca
    23341 accatgacta cggtgatgtc tttacggatc tgctcgccca cttcgacgat gttgaaaccg
    23401 tgcgagtaac ccagcgccgc gccgtctttc atcagtggct gtacggtgcg cactacatca
    23461 gagtgctgct tgtccggcgt caggttaacc accagatccg cctgtgggat cagttcttcg
    23521 taagtaccca ctttaaaacc attttcggtc gctttacgcc aggacgcgcg cttctcggca
    23581 atcgcttctt tacgcagagc gtaggagata tcgagaccag aatcacgcat gttcagaccc
    23641 tggttcagac cttgtgcgcc acagccgacg atgactactt ttttaccctg aaggtagctc
    23701 gcgccatcgg cgaattcatc gcgccccata aagcgacatt tgcccagctg tgccagctgc
    23761 tgacgcagat tcagtgtatt gaagtagtta gccatggtga ttcctcgtga tgttgtgctt
    23821 cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa atttattgcg
    23881 gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat tgagggctgc
    23941 gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc gccattttgg
    24001 ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc tcacggcaga ttcagcgcct
    24061 ggaagaagat ctcggccagc cgctgtttgt gcgcgataac cgcacggtga cgctgactga
    24121 agcgggcgaa gaactgcgcg ttttcgccca gcaaacgctg ttgcagtatc agcagctgcg
    24181 ccacaccatc gaccagcaag ggccgttgct ctatggcgaa ttacatatct tctgctcggt
    24241 aaccgccgct tacagccatc tgccgccgat tctggatcgc ttccgcgcgg aacacccgtc
    24301 ggtggagatc aaactcacta ccggtgatgc ggcagatgca atggaaaaag tcgtcacagg
    24361 tgaagctgat ctggcgattg ccgggaagcc agagactctg cccggcgcgg tggcgttttc
    24421 gatgctggag aatctggcag tggtgttgat tgcacccgca ttgccctgcc cggtgcgtaa
    24481 tcaggtttct gtcgaaaagc cggactggtc aacggtaccg tttatcatgg ccgatcaggg
    24541 gccggtgcgc cgccgcattg agttgtggtt ccgacgcaat aaaatcagta acccgatgat
    24601 ttacgccacg gttggcgggc atgaagcgat ggtatcgatg gtggcgctcg gctgtggcgt
    24661 ggcattgttg ccggaagtgg tgctggaaaa cagccccgaa ccggtgcgta accgcgtgat
    24721 gattttagag cgcagcgatg agaaaacgcc gtttgagctg ggcgtgtgtg cgcagaaaaa
    24781 gcggctacat gagccgctaa ttgaggcatt ctggaagatt ttgccgaacc attaacccgc
    24841 caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc ccagctcatt
    24901 cagccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca gtacgcgccc
    24961 gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg tacccagcgt
    25021 gttgaggaag cgcagcagcg cgcccggtga ttccgggaat tcgaagctgt agaggcgttc
    25081 ctgcaacgga tgtgacggac gcccgccgac catatagcgc acatgcaact tcgccatttc
    25141 gtcgtcggag agatccacca cgctgtagcc gccgtcgttg agcatctgca aaatttcttt
    25201 gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag atgcaggcgt ttttggcatc
    25261 ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca agcagttggc agaatttgag
    25321 gaagctgcct ttttcttccg gaatggtcac cgccaacaac gcttcacgct gttcgcccag
    25381 ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc acgttggcac cggaaagaat
    25441 atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca ttcccgccag
    25501 cgccagcgcg ccagagggtt ccgccaccgc acgcacatct tcgaataaat ccttcatcgc
    25561 cgcacagatc gcatcgctat cgacggtgat gatgtcgtcg agatactcct ggcataaacg
    25621 gaaggtttcg tcaccgatgc gttttaccgc gacaccttcg gcgaacagtc ccacgcgtgg
    25681 cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt cttccgcttc
    25741 tacggcgatc actttgattt gcggcatcag ttgtttgatc agcaccgcta cgcccgccgc
    25801 cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggagtcct gctggagcag
    25861 ttccagcgcc agcgtgcctt gcccggcaat caccatcgga tggtcgaacg gcggcaccca
    25921 ggtgaacccc tgctgctgtg acagttcgat cgctttagct ttcgcttcgt caaagttcgc
    25981 gccatgaagc agcacttcgc cgccgaatcc acgtaccgca tcgactttga tgtcggcggt
    26041 ggcggttggc ataacgatca gggccttcac gcctaaccgc gcagaagaaa acgcgacgcc
    26101 ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc gctttttgtt cttccgtcag
    26161 gcccgccatc atggcgtatg cgccgcgcag cttaaagctg tgcactggct ggcgatcttc
    26221 gcgcttcacc agaatgacgt tatcaagacg cgacgacagt ttttccattt tttgtagcgg
    26281 cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact gctcttaaat attcggcacc
    26341 ttccggagca ccggacaggg gttgcgagtc agccattatt aaccccccag tttcgattta
    26401 tcgcgcaccg cacctttgtc ggcgctggtt gccaggctgg cgtaggcgcg cagggcaaag
    26461 gaaacctgac gttcacggtt tttcggcgtc caggctttgt caccccgagc ttcctgcgct
    26521 tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg gttcgggatg
    26581 tcgatagcga tcaggtcacc atcttcaatc agaccaatgc tgccgccgct tgccgcttcc
    26641 ggtgagacgt ggccgatgga aagaccagag gtgccgccag agaaacgacc gtcggtgatc
    26701 agcgcacagg ctttaccgag acccattgat ttcaggaagc tggttgggta gagcatttcc
    26761 tgcatgccag ggccgccttt cgggccttca tagcggatca ccaccacatc gcccgcgaca
    26821 actttgccac cgaggatcgc ttctaccgcg tcgtcctggc tttcgtacac tttcgccggg
    26881 ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt tcacgatgca gccgttttca
    26941 gcaaaattac cgtagagcac cgccaggccg ccgtctttgc tgtaggcgtg ttccagcgag
    27001 cggatacagc cgtttgcgcg atcgtcatcg agagaatccc aacggcaatc ctgcgagaat
    27061 gcctgtgtag tacgaatgcc cgcagggcct gcgcggaaca tattttttac cgcgtcatcc
    27121 tgggtcagca taacgtcgta ttgttccaga gtttgcggca acgtcaggcc aagtacgttt
    27181 ttcacatcac ggttcagtaa ccccgcgcga tccagttcac cgagaatacc gataacacca
    27241 ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcaac tttgcataac
    27301 tgcgggactt tacgcgaaag tttgtcgata tcactcatgg tgaagtcgat ttccgcttcc
    27361 tgcgccgccg ccagcaggtg aagtaccgtg ttggtagagc cgcccatcgc gatatccagc
    27421 gtcatggcat tttcaaacgc cgctttgcta gcaatattgc gcggcagtgc actttcgtcg
    27481 ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt taccggcatt gaggaacaac
    27541 tgcttacggt ccgcgtgggt tgccagcagc gagccgttgc ccggctgcga caggcccagc
    27601 gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga gccgcaggtc
    27661 ggacacgcgg aacgttcaac ctgatcgctc tgggagtcag aaactttcgg gtctgcgccc
    27721 tggatcatcg catcaaccag atcgagtttg attatctgat cggacagttt ggttttcccg
    27781 gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag ggaagccatc
    27841 agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc ggcgcagtgg
    27901 gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggaag tgaataaagc
    27961 atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa ctctttggca
    28021 acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg cagatggacg
    28081 tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt gccaaaatcg
    28141 gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt acgaccatga
    28201 gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc tgtctcgtaa
    28261 atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat ctaaccagcc
    28321 ccatttatct tcggtttcgc cagtgaagag gccgaagaag gcttgctgaa tgcgtttggt
    28381 aaccgggcca caacggcctt cgccaatctg aataccgtct acgctgcgca ctggcgtgat
    28441 ttctgccgcc gtaccggaca taaagacttc atccgccaag tacagggatt cgcgcgacag
    28501 cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg catcacgggt
    28561 aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat ctttcacttc
    28621 aaacaggttt tcgcctgcgc cttcagagat ataaccgttc acatccagcg cgataccttc
    28681 ctgataaccg tggcggcgcg cttcgctacc caccagcagg gaagagagat agttaccacc
    28741 ggcttttgcc gcagttggga tggtgtttgg tgctgcgcgg ttccaggagg aaaccatcgc
    28801 atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga aagcggcgat
    28861 aatcacatcg gttgagtatc ccgctggcgg gtttactccc atgccaacat caccgacgaa
    28921 gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt cacgacaagc
    28981 ttccatcagc tcatcaatgc tctgtgaaac cgggaagcga tagattttgg cggagtcatg
    29041 cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgtg agtcgtagca
    29101 acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca catgcacctt
    29161 cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt tcttcgtggt
    29221 catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt gtgctctggc
    29281 agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt aagtcgaccg
    29341 accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg gcggcagcca
    29401 tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca cgttctaagg
    29461 tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata atttctccaa
    29521 catttctgaa ttactggcac ctggcggcac cagcggccag acgttctcaa gttcgtcgat
    29581 tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg ccgcttcaac
    29641 ctggtcttta cgggtaatgt gttggccagg gatgccgaag gcgctggcta acatgaggaa
    29701 atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tgaaaaaaca gttgctgcca
    29761 ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg gtaactgctt
    29821 gcgttttacg gtgcccagct cttgcacatt catcatgaaa gagccgtcac cggagataca
    29881 gacaacggta tcgttcggtc gcgcgacttg tgcgccaact gccgccggta aaccaaaacc
    29941 catggtacct aagccgctgg aggtgatgaa attttccggg cgagtgtggg cgatgtgctg
    30001 cgcagcccac atctggtgct gccccacatc tgtggtcacg acgcaatccg caggtttacg
    30061 atccgacagt tgttttaaca acaacggagc gtagatagcg tcaccgggat ggtcgtaacg
    30121 ccaggaatgt tcatcacgca gctgcgcgca gtgttgctgc cagtcattga tatttaacgg
    30181 ctgctgtaat gctggtaaca gagcatttaa atcaccttgt aatgccacat gtgcctgacg
    30241 cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt gtggtgcgaa
    30301 ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca cccacggcga tcagcaggtc
    30361 acactcctgc accgcgaagt ttgccgcttt ggttccgtgc atccccagca tgcccagata
    30421 gtacggataa tctgcttcta ctgcgcccag ccctttcagc gtacaggtgg caggcatttt
    30481 tgtggcagcg agaaattcac gcaaagccgg aactgcctgc gccatcccca cgccaccgcc
    30541 aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct cgacttcggc
    30601 atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccagat cgccgctggc
    30661 taactggata tcttttggga tatcgaccag aaccggacca ggacgacctg agctggcaac
    30721 gtcgaatgct tcagccatga tgcgcggcaa ctcttccaga gactgcacca gaaagctgtg
    30781 cttggtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt cggtgccgat
    30841 aaacggtgcg gacacctgac cagtgatggc aacgacaggg atggaatcta acagtgcgtc
    30901 cgcaagcccg gttatcaggt tggttgcgcc cggaccagac gtggcgatac atacgccagt
    30961 tttaccggta gcacgggcat aaccgatagc cgctattgcc gcaccctgct catgtcggca
    31021 cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa ttgcgccacc
    31081 cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca cccactgtgc
    31141 gccattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt gctgttattc
    31201 attgtctgct cctcggttat gtttttaagg tcaaaaaaac ccccggacct ttcggtgcgg
    31261 gggtcttagt tcgttaaggc ttgatctcta agcctttcct cgtccaagtg cagccccgca
    31321 cggtgggata ataatcacca ccacgctaat cacgaccagg ctaatcactc gtagaagggc
    31381 tgtcattttg tcttttcttg catcttgttc gaaggaatgc ctaaagagtt accatagatt
    31441 ttgcgaatag cacaagatat ttttgggcct atttttatga tggctttaat aatttcaata
    31501 gaaattgttg ttttttaaaa agaaaatagt gagtgaaaaa attcaatttc agtataaatg
    31561 ttgttccctt cctcgtagtt catcctttgt ccgctgcatt tttgcgagca tcattccacc
    31621 gccagaacaa ctcaacttca cttgcacaaa taaacaggaa tcagccacta aaattcacga
    31681 attagcgggc tacagcagcg atatcttatg cacataatgg tgactcaagg agggtttatg
    31741 tcactgtcaa ttgttcatac ccgcgcagcc ctggaagtaa atgcgccccc aatcactgtt
    31801 gaggtacata tcagtaaagg tctacccggc ttaacgatgg tgggcttaac agaaacaacg
    31861 gtgaaagaag ctcgcgatcg cgtgcgcagc gccattatca atagcggata tgaatatccg
    31921 gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc caaaagaagg gggacgatat
    31981 gatttaccta tcgccattgc gttgctggcg gcctcagaac agcttacagc caataagtta
    32041 gatgaatatg aattagtcgg agaactagcg cttacaggcg ctctgcgtgg cgttcccggt
    32101 gcaatctcca gtgcaactga agctattaag tcgggcagaa aaattatcgt cgcgaaagat
    32161 aacgaagatg aagtggggct aattaacggt gaaggatgcc tgatagccga tcatctgcag
    32221 gctgtctgtg cgtttctgga aggtaagaac actctcgaac gcccgaaacc aactgatgca
    32281 gtatcccggg cgctacaaca tgatctcagt gatgttgtcg gtcaggagca aggaaaacga
    32341 ggactggaaa ttaccgccgc tggtgggcac aaccttttac tgattgggcc gccgggaaca
    32401 ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc cagatttaag caatgaagag
    32461 gcactggaga gcgctgcgat attaagtctg gtaaatgctg aatcagtaca aaaacaatgg
    32521 cggcagcgcc cgttccgctc acctcatcac agtgcgtcgt taactgcgat ggtgggcggt
    32581 ggtgcaattc cagggccagg tgaaatttcg ttggcgcata acggcgtgct ttttcttgat
    32641 gagctacctg aatttgaacg gcgtacactg gatgccttgc gagagccgat tgaatccggg
    32701 cagatccatc tttcacgcac acgagcaaaa ataacctatc cagcccgttt ccagcttgtt
    32761 gcggcgatga atcccagccc taccggacat tatcagggaa accataaccg ctgcacgcca
    32821 gaacagacat tacgttatct caaccggctc tcggggccct ttctcgaccg cttcgatctc
    32881 tcactggaga tcccattacc accccccggc attttgagta aaacggtagt gccgggagaa
    32941 agcagcgcca ccgttaaaca acgcgtaatg gccgccagag agcgccaatt taagcggcag
    33001 aataaactga atgcctggct ggatagtccg gaaatacgcc aattctgcaa acttgagagc
    33061 gaagatgcga tgtggttgga agaaacgctg atccatctgg ggttatcgat tcgtgcctgg
    33121 cagcggttac tgaaagttgc acgaaccatt gctgatattg atcagtctga cattatcaca
    33181 cgtcagcatt tgcaggaggc agttagctat cgtgcgattg accgtttgct catccatctg
    33241 caaaaactac tgacataaaa aaagggcatt tcgccctttt tattaatcgt cagaatcggt
    33301 gtagtcttca gcaccttcaa cctgcggttt accgccggaa agggtgtgaa aacgttttgg
    33361 acgcttaata cgcgtcatat acttggacca cacgcgttct gcttctgtca ctggctcacg
    33421 ttcgccacgg catactgcta cgaagagttt ctcttcctcg gtaaccggct cgcgtttgcc
    33481 aagatccaac tcattgaagg cataaccatg acgctcaagc agttgtgcct ctttgatggt
    33541 gaaatcacca tgacgagaga atccacgtgg ataatgttta ttgtcgaaat atcgattagt
    33601 cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct ttgaccgagc tagttatggc
    33661 gcggagtatt agttacgctt gacagagtgt aaaacaaaac atttaaatca taacgacaaa
    33721 taattttgcg gagagcactg tggatacgga attgttaaaa actttcctgg aagttagccg
    33781 aacgcgtcac tttggtcgag cggctgaatc gctctatctg acccagtcag cagtgagctt
    33841 tcgaatcaga caactggaaa atcaactggg tgtgaacctt ttcacccgcc acagaaacaa
    33901 tatccgttta accgctgccg gtgaaaaact actgccttat gcagaaacgc tcatgagcac
    33961 ctggcaggcc gcccgcaagg aggtggcgca tacctcacga cataacgagt tttctatcgg
    34021 tgccagcgcc tcgttgtggg aatgtatgct taatcagtgg ttgggacgct tgtatcaaaa
    34081 tcaggatgcc catacaggct tacagttcga agcgcgaatt gcccaacggc agtctctggt
    34141 aaaacagctg catgaacgcc agcttgatct tcttattacc actgaagcgc ccaaaatgga
    34201 cgaatttagt agtcagttgc tggggtattt cactttagcg ctttatacca gtgccccttc
    34261 taaactaaag ggagatctta actatctgcg actggagtgg gggccagatt ttcaacagca
    34321 tgaggcaggt ttgagcggtg ctgacgaagt tcccattctg acaaccagtt ctgctgaact
    34381 ggcacagcaa cagattgcga tgcttaatgg ttgcacctgg ctacccgtca gctgggcgcg
    34441 taaaaaaggc agcctgcata ccgttgtcga tagcacaaca ctttcacggc cgctttatgc
    34501 catatggctg caaaatagcg ataaaaatgc gttgattcgc gatcttttga aaattaacgt
    34561 gctggatgaa gtgtattaat ctgaatggct ggcaaggatg ccggtagaag gatttacttc
    34621 ggagaggatt atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggcag
    34681 gggcggagag actcgaactc ccaacacccg gttttggaga ccggtgctct accaattgaa
    34741 ctacgcccct aattagggtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga
    34801 caggcaggta ttctaaccga ctgaactacc gctccaccga attcttttac aaccaccggt
    34861 tttatg
//
LOCUS       DADLXX010000029        34281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLXX010000029 DADLXX010000000
VERSION     DADLXX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.029"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(93..2189)
                     /locus_tag="JNX27_001408"
     CDS             complement(93..2189)
                     /locus_tag="JNX27_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7882664.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYARELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2191..2442)
                     /locus_tag="JNX27_001409"
     CDS             complement(2191..2442)
                     /locus_tag="JNX27_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7882665.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2595..3158
                     /locus_tag="JNX27_001410"
     CDS             2595..3158
                     /locus_tag="JNX27_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY7882666.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3434..3856)
                     /locus_tag="JNX27_001411"
                     /pseudo
     CDS             complement(3434..3856)
                     /locus_tag="JNX27_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(3856..4449)
                     /locus_tag="JNX27_001412"
     CDS             complement(3856..4449)
                     /locus_tag="JNX27_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7882667.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4596..5003
                     /locus_tag="JNX27_001413"
     CDS             4596..5003
                     /locus_tag="JNX27_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7882668.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5123..6115)
                     /gene="rpoS"
                     /locus_tag="JNX27_001414"
     CDS             complement(5123..6115)
                     /gene="rpoS"
                     /locus_tag="JNX27_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7882669.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6178..7317)
                     /gene="nlpD"
                     /locus_tag="JNX27_001415"
     CDS             complement(6178..7317)
                     /gene="nlpD"
                     /locus_tag="JNX27_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7882670.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGAQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7457..8083)
                     /gene="pcm"
                     /locus_tag="JNX27_001416"
     CDS             complement(7457..8083)
                     /gene="pcm"
                     /locus_tag="JNX27_001416"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7882671.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8077..8838)
                     /gene="surE"
                     /locus_tag="JNX27_001417"
     CDS             complement(8077..8838)
                     /gene="surE"
                     /locus_tag="JNX27_001417"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7882672.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8819..9868)
                     /gene="truD"
                     /locus_tag="JNX27_001418"
     CDS             complement(8819..9868)
                     /gene="truD"
                     /locus_tag="JNX27_001418"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7882673.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARNALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9865..10344)
                     /gene="ispF"
                     /locus_tag="JNX27_001419"
     CDS             complement(9865..10344)
                     /gene="ispF"
                     /locus_tag="JNX27_001419"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7882674.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10344..11054)
                     /gene="ispD"
                     /locus_tag="JNX27_001420"
     CDS             complement(10344..11054)
                     /gene="ispD"
                     /locus_tag="JNX27_001420"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7882675.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11073..11384)
                     /gene="ftsB"
                     /locus_tag="JNX27_001421"
     CDS             complement(11073..11384)
                     /gene="ftsB"
                     /locus_tag="JNX27_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7882676.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11578..11901)
                     /locus_tag="JNX27_001422"
     CDS             complement(11578..11901)
                     /locus_tag="JNX27_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7882677.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11951..12556)
                     /gene="cysC"
                     /locus_tag="JNX27_001423"
     CDS             complement(11951..12556)
                     /gene="cysC"
                     /locus_tag="JNX27_001423"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7882678.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12556..13983)
                     /gene="cysN"
                     /locus_tag="JNX27_001424"
     CDS             complement(12556..13983)
                     /gene="cysN"
                     /locus_tag="JNX27_001424"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7882679.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13985..14893)
                     /gene="cysD"
                     /locus_tag="JNX27_001425"
     CDS             complement(13985..14893)
                     /gene="cysD"
                     /locus_tag="JNX27_001425"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7882680.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15145..16182
                     /gene="iap"
                     /locus_tag="JNX27_001426"
     CDS             15145..16182
                     /gene="iap"
                     /locus_tag="JNX27_001426"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7882681.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16268..17419)
                     /locus_tag="JNX27_001427"
     CDS             complement(16268..17419)
                     /locus_tag="JNX27_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY7882682.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(17613..17765)
                     /locus_tag="JNX27_001428"
     CDS             complement(17613..17765)
                     /locus_tag="JNX27_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7882683.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18029..18763)
                     /gene="cysH"
                     /locus_tag="JNX27_001429"
     CDS             complement(18029..18763)
                     /gene="cysH"
                     /locus_tag="JNX27_001429"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7882684.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(18837..20549)
                     /gene="cysI"
                     /locus_tag="JNX27_001430"
     CDS             complement(18837..20549)
                     /gene="cysI"
                     /locus_tag="JNX27_001430"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7882685.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20549..22348)
                     /gene="cysJ"
                     /locus_tag="JNX27_001431"
     CDS             complement(20549..22348)
                     /gene="cysJ"
                     /locus_tag="JNX27_001431"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7882686.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22664..23029
                     /gene="queD"
                     /locus_tag="JNX27_001432"
     CDS             22664..23029
                     /gene="queD"
                     /locus_tag="JNX27_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7882687.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            23107..24378
                     /locus_tag="JNX27_001433"
     CDS             23107..24378
                     /locus_tag="JNX27_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7882688.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24369..24629
                     /locus_tag="JNX27_001434"
     CDS             24369..24629
                     /locus_tag="JNX27_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7882689.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24646..25221
                     /locus_tag="JNX27_001435"
     CDS             24646..25221
                     /locus_tag="JNX27_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY7882690.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25369..26262)
                     /locus_tag="JNX27_001436"
     CDS             complement(25369..26262)
                     /locus_tag="JNX27_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7882691.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADID
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(26226..27005)
                     /locus_tag="JNX27_001437"
     CDS             complement(26226..27005)
                     /locus_tag="JNX27_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY7882692.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26983..28392)
                     /locus_tag="JNX27_001438"
     CDS             complement(26983..28392)
                     /locus_tag="JNX27_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7882693.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28414..29868)
                     /locus_tag="JNX27_001439"
                     /pseudo
     CDS             complement(28414..29868)
                     /locus_tag="JNX27_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29938..30724)
                     /locus_tag="JNX27_001440"
                     /pseudo
     CDS             complement(29938..30724)
                     /locus_tag="JNX27_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31042..32319
                     /locus_tag="JNX27_001441"
     CDS             31042..32319
                     /locus_tag="JNX27_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7882694.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     HGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32346..33824
                     /locus_tag="JNX27_001442"
     CDS             32346..33824
                     /locus_tag="JNX27_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7882695.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVLGHITPQGALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         8282 a         9075 c         8423 g         8501 t
ORIGIN      
        1 tcatcaatat cagtcatagt atttcctcgg ttatttcaaa tcagctttgt ttcctttatt
       61 taacagtccg ttcccgcgga ctgttttttt attcaggcca gcggatcatc ttcttcctgc
      121 atggcaaaaa gtttttgcat ccccagccgg aaatcgcgtt caaattcttc gtcatgctct
      181 tcttcatatt ccggttgctg aactgccacc acattaccaa ccgggcgata aacattactc
      241 agccagggtt cagcctgttt gtgttccaga atctgaatac cttcatcttc agcatcacga
      301 attttttcct cagcgcgttt gcgcattcct ttaatacgct gctgttgttt gtgatattca
      361 gcactgacag caaaggctgc gcgtttattg ccgttccata tcgcctcaca gataaagctg
      421 ccgtccttgc gacgtaccgt aattcgctcg gcgtcatgaa tgtcataact gataagcact
      481 ttgcgaccgt gttcgtcacg caactcccgc gcgtagtaaa tattgttcag ccactgaatt
      541 tcgcagcgtc ttaccggtcg ttccaccatt gggcggaaca tgtcgcgcag ctcaagatca
      601 ctcagccact caatttcggt gtcttcttct gccaggcgtt tttttctgaa ctctgtcggg
      661 ctgtaatgcc gtccgttcgg tttcatcggc agctcagaat gcgggcggtt gttgtaccat
      721 tccacgcctt cacgaatagc ctcaataagc tccgcccacg atggcagctc gcgcattgcc
      781 gctttctggc ggtcgttcag aggcttgtcc tgttgcaggg cgttaaacgc tgaacgcaga
      841 tcacggttaa gtacacgcaa cgattcccgg tcagcaccct tgccaaaata ggtgcgatac
      901 ctcctggcta tacgcatcgg cagtgtccgg ttcaggcgtt caataatgcc gcgtccctgt
      961 ggattaccgg caataccggt agggtgttta atccccaggc gtggcagaat acccacgacc
     1021 tctttgtcca gcatgtcagc cgtttcaccg gaaccgttat ccgagtaata caggaacggt
     1081 tttccgtggt tgcgaatgcc gtgctgtatc gcaccggcaa cggcgaacac gttttcggca
     1141 aggtcaagac tccagccaac aacaaaccgt gttccaccat cgatcacaaa cgtgacttcc
     1201 ggtgagaatg gacgcccgtg gatcggatgc gcacatttca gcttcatacc gtgaccgtca
     1261 ccaatccaga cataattcac cggcatagct gtccagtcgc ggcgggtgaa tccttcaagc
     1321 tgacggtatt cactgccggt aacgcgccct ttttgtttca ccacttccgg cagttttttc
     1381 atggcatacc ggacaacatc ataggaaggc atcacctcca gcatgtacgg ttcgtccgca
     1441 tggcgacgct gccattctgc gacaaaatcc tcgtaggctt ctgacatggg tctgccgttc
     1501 acctggcgat actgcgccag aaattcaggc agccagctga tttcttccgg ttttatttcg
     1561 tcgcgttttc ccggtgccag taacagcagc cgttcggcgg cgttctgcga ctggttgtat
     1621 ttcagaaccc actggtaaag tggatcgcgg ctgatagtac gattagcgcc acgttttgca
     1681 ttggcgttaa ctgcggcctg ttgcaggcgc tccggcagct cacctttacg ggctttttca
     1741 acaagaaagt taatggcttt cagtcgcccg aggttaccgg cgctttccag cttcagcact
     1801 tccaccacca gcgcagctct tgcttcagat acccagcgct ggttttcggt cagcgcattc
     1861 aggcgttctt ccatcagttg tggagaaccg cgatatgctt cgattgcctg gacagcgtca
     1921 ggattgcgtg tttttctggt aacagctttc acttcagaaa cattgttctc tgtagccacc
     1981 agctgttcca catagcgttc acgcagtgcc tttcgtgtta tttcaggcag acaatcaatg
     2041 tggtactcaa tggcttttgt gccttcccga cggcgactaa cgtcaccaag atcacgagaa
     2101 aaacgctgta acgcctgtcg gatgcctttt gtggtggctg gcattcctgg tacgccgagc
     2161 aggtcattta cggtaacgta cgactgcata tcacgacgcc ttcctgataa atccatcgtt
     2221 acgataccgg ctgggccaga tgatttctgg cggtacacct atagcgtcag aaattacgcg
     2281 ttcgtatttt ttgtttggtg tgcgaactac cgctttcagt gaatcctgag cataaccagc
     2341 atggagtgaa agctcccgga aggaagacca cccgttatct ttgagcgcca gctgaatctt
     2401 cagcgaaggc caatcgcggt cactatcttc tctttgtctc atctttcctt taacctacaa
     2461 aataacccgc gcggatatcc gtatggataa agattaagat accaaagatt ccctataaag
     2521 ggttctttgg tgcttttatg gtttattttt taatttcatt atttatcaat gaattagata
     2581 gaaaggaacc aagaatgaga aaagcacccc aaggttcttt tcctgccgat ggaaaagaac
     2641 cgattatcga aagaatcttt aggttagtag aacgataccc ttcaagaaat gcggctgctc
     2701 gggcatgggg gatcaatgaa ggcactttaa aaaattatta caacaggcgg gattacgcac
     2761 cgataccacg cccgcatcag ttaaaaaaga tagcagaaag cgaaaatgtt accttggaat
     2821 ggctcatgac gggagagggt gatgttgaac taaaagattc caaagaaccc aaaaaagaga
     2881 atgcggataa tgttgatgct caaattctaa tgttcttgtc atttcttaaa ccacaagaaa
     2941 aacaacaagt agctgatgtt cttgggagaa aaggagcgga gcaattactc attcttctgg
     3001 atgagcacat ccaagagttg cacgctctag ttggagttcg tagagccata gcgttaagcc
     3061 ttggtaattt gccagacgaa aaggttagag agatttacgc gttgtccgaa gctaagggcg
     3121 atcacttaaa tctacctcag aaagcagcca gcgcataacc cagtgcaagt gcctagctgt
     3181 gctaaaatta cattggtttt ggcgcttgca cccatcagcg ctataataaa cacctgaatc
     3241 gtttttgaac ggtctttaaa ggctattgta agaacctgtg agttatcttg aatgttttat
     3301 tgatatcgaa ccgtttaaca tttttggact cgctttatct tgaaaacctt gagttcctaa
     3361 cttgttgatt ccaatctatt cccgtctttt ttaatctctt cccggttatc ttgaataaat
     3421 tacctagtca tacgagatag aaaccgccat cgccatcatt caggcgaaac agcggcagaa
     3481 tatcgaacag gttgatctct tcgccatcga cggtgttctg cgcccaggct ggattggcgc
     3541 ggcgctccgg ggcaatcggg aagttatccc agcggcggat aaactcatca atctgctttt
     3601 tgaccggggt gtttggtggc aggccaaggg aaatcgcgtg gttctgccag gaaccgatgg
     3661 tattcatcgc cacgcgggca tcggtaaagc cgcgaatatt atcaaaccac agcgcgggcg
     3721 cgccgtcgcc gatacgcccg gtggcgttag ctgctgctgc cagatccggc tcagcgttca
     3781 cttcttcgct gattttcagt aactggccgt ggtcatcaag cgcctgtaaa aagctgcgta
     3841 aatcatcaaa tgccattatt cattctcctg agaaaaattc cgggcctgcg gcaatccttg
     3901 ccagcgcctg gcgtggggat gttcgaggcc aaattgatcc agcacgcggg ctaccacatg
     3961 gtggacaatg tcatctaccg tttcgggatg gttataaaag gcaggcatcg gcggcaccat
     4021 cgccacgccc atgcgtgaaa gtgcgagcat attttcgaga tggatggtgc taagcggcat
     4081 ttcacgcggc accagcacca gtttgcggcc ttctttgagc acgacgtccg ccgcgcgccc
     4141 taccaggcca tcggcgtaac cagcgcggat accggcgagc gttttcatac tgcacggaat
     4201 aacgatcatg ccgtcagtac gaaaagaacc ggatgagatg atcgccgcct gatccgccgg
     4261 gttatggctg aagtctgcga gggcagcaac atcgcgagcg ctgtaaggcg tttccagttc
     4321 aatgatggtt ttcgcccact tcgacatcac cagatgagtc tcgacattcg gcatctcacg
     4381 cagcgcttgc agtaatgcca caccaagagg cgcaccggta gcccctgtca tcccgacgat
     4441 cagtttcatt gcttattctc ttaattattc gcatacgaac gttattatta acatactcct
     4501 tcatttccac agcggcaagg attgaaaagt aacaacatct tcttcgtcac taaaaaaccg
     4561 ctatgatagc gacagagaat tagcccggag tttacatggc gttacgaaat aaagcgtttc
     4621 atcagttacg acagcttttt cagcagcaca cggctcgctg gcagcacgag ttacctgacc
     4681 tgaccaaacc acagtacgcg gtgatgcgcg ccattgctga taaacctggt attgaacagg
     4741 tggcactgat agaagcagca gtcagcacca aagcaacgct agcagaaatg ttggcaagaa
     4801 tggaaaatcg tggcttagtc agacgtgaac atgacgtcgc tgacaagcgg cgacgctttg
     4861 tctggctgac tgccgaagga gaaaagctac ttgctgcggc gatacctatt ggtgacagcg
     4921 tggatgagga atttttgggg cgtttgagtg gagaagagca ggagctgttt atgcagttgg
     4981 tgcgcaagat gatgagcaca tagaatgctg attgccgggt aggacgctga cgtgtcttat
     5041 ccaggcgaca aaaacacaag cttcccaaac aaaagaaaaa ggccagcctc gcttgagact
     5101 ggcctttctg acagatgctt acttactcgc ggaacagcgc ttcgatattc agcccctgcg
     5161 tttgcaggat ttcgcgcaaa cggcgcaggc cttcaacctg aatctggcga acacgttcac
     5221 gggtgaggcc aatttcacga cctacatctt ccagtgttgc cgcttcgtac cccagcaaac
     5281 cgaatcgacg tgccagtact tcacgctgtt tggcgttcag ctcgaacagc catttgacga
     5341 tgctctgctt catatcgtca tcttgcgtgg tatcttccgg accgttctct ttttcatcgg
     5401 ccaggatgtc cagcaacgct ttttcggaat caccacccag cggggtgtct accgaggtaa
     5461 tgcgctcgtt aagacgaagc atacggctga cgtcatcaac tggcttatcc agttgctctg
     5521 cgatctcttc cgcacttggt tcatggtcca gcttatggga caactcacgt gcggttcgca
     5581 ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg aatagtacgg gtttggttca
     5641 taatcgcccg ttcaatcgtc tggcgaatcc accaggttgc gtatgttgag aagcggaaac
     5701 cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc caagttgccc tcttcgataa
     5761 ggtccagcaa cgccagacca cgattgccat aacggcgggc aatttttacc accagacgca
     5821 agttactctc gatcatccgg cggcgagagg cgacatctcc acgcagtgcg cgacgcgcaa
     5881 aataaacttc ttcttcggcc gttaacagtg gtgaataacc aatctcacca aggtaaagct
     5941 gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa cagttcctct tcggccaaat
     6001 cgttatcact gggttcctct tctactaagg ccttttcgtc aaaaacctca actccgttct
     6061 catcaaattc cgcatcttca tttaaatcat gaactttcag cgtattctga ctcataaggt
     6121 ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa agcctggttc cgccgattta
     6181 tcgctgcggc aaataacgca gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg
     6241 caagcgtgtt gaactggttc cggtgctacc catggttgct attttttgcc ccgccttcac
     6301 ttcttgttgt tcccggacca gcattgtgtc gttatgggcg taggcactca ggtaatcatc
     6361 attatgtttg atgataatca gattaccgta gccgcgcagc gcgttaccag cataaacaac
     6421 gcggccatct gcggtcgcga taattgcctg tcctttgctg cctgcgatat caatcccctt
     6481 gttgcccccc tcagaagcgc caaaggtttc gatcactttg ccctcagtcg gccagcgcca
     6541 ggtggagata ggcgtactgg ttgatgtact gctgacagtc ggctcggttg tgcttgctgt
     6601 tggtaccgtt acaggcgctg tgaccgtggt cgcagttggc ttgttgttcg gcaacatttt
     6661 gttagcactc tgtgcacccg aagactcaga atacgtaatt gtcggttgcg acgcaacagc
     6721 aacggtggaa ttttgtgcag gcttgatcac aactccttgc tctgctgcgt cggcctgggt
     6781 aatagcattt ccgccagtga ttggcgtacc ggaagcatta cccacctgca aggtctgacc
     6841 aacgttcagc gcgtatggtg cctgaatatt gttgcgctga gcaaggtcac ggaaatcgtt
     6901 gccagtaatc caggcgatat agaaaagtgt gtcacctttt ttcacggtat aggtactgcc
     6961 gctataactg cctttcggaa tgttcccata ctgacggtta tagacgatgc gtccgttttc
     7021 catctgtacc ggctgctgag ctactggctg cactggctgg atttgcggtt gttgagtagc
     7081 ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc tgcgctgtag acgtcgtccc
     7141 tattttcggc ggcggcgtaa tcaacatacc agaattagta tttgcaggcg cattgccatt
     7201 aacggagctg accggtgccg gtggatttga agtgtcagaa cagcctgcca gccatagcga
     7261 aaccagtgac aaagccgcaa tgcggcgaac ggtgaatttt gggcttcccg cgctcattta
     7321 tcccccagga aaaattggtt aataaccagt gacataatta ccgtgcaagg caccctactg
     7381 aacactggaa aagatgttca cgatacgctg acctacggca aaataaccag gaaaaatcca
     7441 ggtatttcct cacgttttaa gccagctcac ccttcactaa agggacaaag cgcacggcct
     7501 ccacggtatc gataataaat tcgcctcccc gacgacgcac ccgtttcaaa tactggtgct
     7561 cctcccctac gggtaagacg agaatcccgc cttcgtccag ctgcgtcatt agcgcagttg
     7621 gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa cggcgcacgt gcctgccaac
     7681 cttgccatcc atcaccatga cgggttgaaa cattatgtaa atcaagattt ttcaggcggc
     7741 gacgtgcctg ccactgcaag cctttaatcc gttcaaccga gcaaacatgc tggacaagat
     7801 gcgccaggat tgccgtttga tatcccgaac cagtgccaat ttccagcacc cgcgactgcg
     7861 gcgtcagctc gagtaattcg gtcattcgcg ccaccatata cggctgcgaa attgtctgcc
     7921 cctgacctat cggcaaggcg atattgtccc aggctttttg ttcaaacgct tcatcaacga
     7981 atttttcacg cggcacggcg gcaagtgcat tcagcacctg ctcatcctga ataccttgcg
     8041 cacgtaattg atccagaagt gcttgtacgc gtctgcttac cattgcgtgc caactcccac
     8101 gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc gcagttaaat ccacatgcag
     8161 cggcgtgatg gagacatagc cctcatctac cgcagcaaaa tcggtccccg gaccggcatc
     8221 acatttaccg cccggcgggc caatccagta cagcgtattg ccgcgcggat cttgctgcgg
     8281 gatcacctga tctgccggat gtcgtgtacc gcagcgcgtc acgcgaatac ctttgatttg
     8341 atccaagggt aaatccggaa cgttaatatt aagaatacgt ccggtgcgca gcggctcttt
     8401 acacagtgcg cgcaaaattg aacaggttac cgccgcggca gtgtcgtaat gtttatgccc
     8461 gtcaagcgag acggccagcg ccggaaaacc taaatgacgg ccttccatcg cggcggctac
     8521 cgtaccggaa taaataacat catcccccag attcggcccg gcgttaattc cggacacaac
     8581 aatgtccggg cgcggacgca tcagagcatt cacgccaaga tagacacaat cggtcggagt
     8641 tcccatttgc acagcaatat caccattttc aaaggtaaac gtgcgcaggg aggattccag
     8701 tgtcagagaa tttgaagcgc tgctgcggtt acgatcgggg gcgaccacct gaacgtcagc
     8761 aaactcacgc aaggctttcg ccagcgtttg tataccgggt gcatgtaccc cgtcatcatt
     8821 actcagcaat atgcgcataa tcacctgttg tgttgataag ttcccttaca acgctggttg
     8881 caaaactacc cgccggaagc cagaaacgga tctctacggt gacgtcatcc caccagttcc
     8941 agcttaattg ttgcggatac agcagcatcg ctctgcgcgc ggcttcaact ttttcgcgca
     9001 ccagtaaagc ttgtaattca gtttctgcgg cgacagctgc ttgttcgaat gccagcgctt
     9061 cacgctgagt tccccattcg ccactgcccg gcaatgcggc ggttatcatc agctctttat
     9121 cgttgacgcg acgctgtaat tccgccagtt cttcggtggt tgctacaaac cagctaccac
     9181 gtccggctaa ttgtagcgca tcgccgtcaa caacttgatt aacgtctgct tttttgaggc
     9241 gctcagcaac aatctgatta aacaacgcat tgcgggctgc cgacaaccaa aaactccgtt
     9301 tattgcgatc gcgcaccgga gtattggttt gcgcccagcg cagcgcgccc tgcaagttgc
     9361 taccgccaat cccaaaacgt tgggcaccga agtagttcgg tacacctttt acgcaaatat
     9421 cgatcagacg ttgttcaacg tcatcgcgat tgctcacttc gcgcagaacc agggtaaagg
     9481 cgttaccttt cagcgcccct aaacgcagct tgcgcttgtg ccgcgcatac tccagcacct
     9541 ggcagccttc cagttgaaag gcgctcagat cgggcatttc cttgcccggc acgcgagcac
     9601 ataaccactg ttccgttaca gcatgtttgt ctttttgccc agcgaagctg acttcacggg
     9661 aatgaatttt caggaatttc gccagtgcat ccgccacaaa acgggtattg cagccgtttt
     9721 tgaggattct aaccagaata tgctcacctt caccatcagg ctcaaagccc aaatcttcca
     9781 ccaccacaaa gtcttccgga ttggctttca gcagcccggt gccttgcggt ttaccgtgga
     9841 ggtaagtgag attatcaaac tcaatcattt tgttgcctta atgagtagcg ccaccgcttc
     9901 acaggcaatc ccttccccac gtccggtaaa tccaagtttt tccgtagtag tggctttcac
     9961 gttaacatca tccatatggc agccgagatc ttcggcaata aacacgcgca tttgtggaat
    10021 gtgcggcaac atcttcggtg cctgagcgat gatagtgaca tcgacgttgc caagggtata
    10081 acccttcgcc tgaatacgac gccaggcttc gcgtagcagc tcgcggctgt cggcaccttt
    10141 aaatgccgga tcggtatccg ggaacagttt gccgatatcc cccagcgccg ccgcgccaag
    10201 caatgcatcg gtcaacgcat gaagcgccac gtcgccatca gaatgcgcca gcaatccttt
    10261 ttcgtaagga atgcgtacgc caccaatgat aattgggcct tcaccgccaa aggcgtgtac
    10321 gtcaaaaccg tgtccaattc gcattatgta ttctcctgat ggatggttcg ggtgaggtaa
    10381 aactcggcca gtgccaaatc ttccgggcgc gtgactttaa tgttatccgc gcggccttcg
    10441 accaactgcg gatgaaatcc gcaatattcc agcgccgagg cttcgtcggt aatggtagcg
    10501 ccttcattta gagcgcgcgt cagacagtca tgtaacagct cacgagggaa aaattgcggc
    10561 gtcagcgcgt gccataagcc gttgcgatca acggtatgag caatggcatt tttgcccggt
    10621 tcggcacgtt tcatagtatc gcgcactggt gcggcgagga tccctcccgt gcggctggtt
    10681 tcgctcaacg ccaacaatcg cgcgaggtca tcctgatgca aacaaggacg agcggcgtca
    10741 tgcaccaata cccactgcac atcgccagcg gctttcagac ctgccagcac ggaatcggca
    10801 cgctcatcac cgccatctac aacggtgatt tgcggatgat tcgccagagg aagttgtgca
    10861 aaacggctat cgccaggact tatggcaatg acgacacgtg tcacccgggg atgcgccagc
    10921 agcgcatgca ccgagtgttc aagaatggtt tgattaccga ttgagagata ttgcttagga
    10981 cattccgttt gcattcgacg gccaaatccg gccgccggaa ccacggcgca aacatccaaa
    11041 tgagtggttg ccatgttaat tcccgggctg atttatcgat tgttttgccc cgcagactgt
    11101 gcgcgcttcg acgcgtcagg caccagacga taaaaagttt cgcccggcct ggtcatgctg
    11161 agttcattac gcgcacgctc ttcgagcgcc tcctggccgc cattgagatc gtcaatttcg
    11221 gcaaaaagtt gatcgtttcg cgctttaagt ttcgcgtttg tagcttgcag tgccgccaca
    11281 tcatcattga cgcgggtata gtcatgtata ccgttcttac cgaaccacag cgaatactgt
    11341 agccagacca gaatagccag caacagcagc gttagtttac ccatcctgcc ccctgaaaaa
    11401 ctgcatcatc atcccatgca tccgaagacg actctacatc ctctgttggg gataccgcga
    11461 caacgcgggc aaatgtacca catttgtcca ttgttacgta tacccagggc gtgcagaaca
    11521 taatctcatt attagttacg gtttgaatta tgaacagagg agacaagaaa gtacaaatta
    11581 gcccagtagc cacataaaca gtgcgccaaa cataatgcct actgtcacca gggtgaaaac
    11641 aatactgtag cgtagctttc cgtccatcaa tgaatgcagc gcaatcccca ccactaccgc
    11701 gacaggcatc agtgccagaa agaaaggcca ggtgtagata aagaagaaca gcgtgttaga
    11761 gccataaatc aacatcggca tcgccagcgc aaataaccag gagataaaac cgaccacggc
    11821 accaggcagt gaccatgtag tttcttcatc ctcagtaagg ctgtcgttat ttgttagtgt
    11881 aatgttatgg ctattacgca tatttgatcc tgttactttg acgaaccggg catggaaacc
    11941 cggtggtgtc tcaggatctg ataatatcgt tctgtctcaa cagatctaat aattgctgta
    12001 ccaaatttgt tactaattgt tcaccattga gatgaatttc tgccgattca ggctcttcgt
    12061 aaacggaatc tattcccgta aagttgcgca gttcaccggc acgcgctttc ttatataagc
    12121 cttttggatc gcgggcttcg caaatcgcca gcggcgtatc gacaaacact tcaataaagc
    12181 gcccttctcc tacgcgttcg cgaaccatct ggcgttcggc gcggtgtggc gagataaatg
    12241 cggtcagcac caccagtccg gctgcaacca tcaaattcgc cacttcaccg acgcgacgga
    12301 tattctcttt acgatcggca tcgctaaaac cgagatcgct gcataatccg tggcgaacat
    12361 tgtcgccatc cagcagatac gtactgacgc cgagtttatg taacgcctcc tccagcgccc
    12421 cggcgaccgt tgatttaccg gacccggaga ggccggtaaa ccacagcact acaccacgat
    12481 gaccgtggtg tagttcgcgt tgttgcacag tgaccggatg actatgccag acgacgtttt
    12541 cgtcatgcag cgccattatt tatcccccag caaatcgcgc gcgccccagt gcggaaagtg
    12601 gcgacgaacc agggcattca attccagttc gaatgcactg aattcagatg acgcagctgt
    12661 tgcctggcta actggctcgt gcaccatacc ggcacccacg gtcacattgc tcaggcgatc
    12721 gataaaaatc agcccaccgg taaccggatt ttgctgatag cgatctaaca ccagtggctc
    12781 gtcaaaagtg agatccacca ggccgatgcc gttcagcggc aggttttcaa cttcgcgttg
    12841 ggtaaggtta ttaatatcaa cctgataacg aatgccatca acacgagcac gcgtcttctt
    12901 accggcaatt ttgatgtcat aactctggcc cggggaaagc ggctgttccg ccatccatac
    12961 cacatccacc gacgcactct gcaccgccgg taacgcttcg tctgccgcca gcagcagatc
    13021 accacggctg atatcgatct catccgtcag caccagggtg atcgcttctc cggcaaagac
    13081 ttcttcgcga tcaccatcaa aagtcacgat ccgcgcgacg tttgattcca caccagaggg
    13141 cagcactttt acacgttgcc cgacttccac gcgaccggat gccagcattc cggcgtagcc
    13201 acgaaaatcg agatttgggc ggttaacgta ctgcaccggg aagcgcattg gctgggcatc
    13261 caccactcgc tggatctcca cggtttccag cacttcgagc agtgtcagac cgctgtacca
    13321 cggcatactt tcactttgcg aagccacgtt gtcaccttcc agtgcggaga gcggcacaaa
    13381 gcggatatcc agattacccg gcagctgccc ggcaaaggtc agataatctt cacgaatacg
    13441 ggtgaacgtc tcttcactgt aatccaccag atccattttg ttgatcgcca cgaccagatg
    13501 tttgatcccc aacagtgtgg agataaaact gtgacgacgg gtttgatcga gcacgccttt
    13561 acgggcatca atcagtaaga tcgccagttc acatgtcgat gcgccagtcg ccatattgcg
    13621 agtgtactgc tcgtgccctg gggtgtcggc gataataaat ttacgcttct cggtagagaa
    13681 atagcggtag gccacatcaa tagtgatgcc ctgttcgcgc tcagcttgca ggccgtccac
    13741 cagcagagcc agatccagct tttcgccctg ggtgccgtga cgcttactgt cgttatgcag
    13801 cgatgagagc tgatcttcgt agatttggcg ggtatcgtgc agcagacgac caatcagggt
    13861 acttttgccg tcatcgacgc taccacaggt cagaaaacgc agcaggcttt tatgttgttg
    13921 cgcaatcatc caggcttcga cgccgccttc attggcgatt tgttgtgcaa gtacggtgtt
    13981 catcttaaaa atacccctga cgttttttca gctccatgga tcccgcctgg tcgcggtcaa
    14041 tcacgcgacc ctgacgttca ctggtggtgg aaaccagcat ctcttcgatg atttccggca
    14101 gtgtttgtgc atttgactcc accgcaccgg tcagcggcca gcagcccagc gtacggaaac
    14161 gcaccatccg ttttttgatc acttcgcccg gttgcaggtc gatacggttg tcatcaatca
    14221 tcatcaacat accgtcgcgt tccagaaccg gacgttccgc agcgagatac agcggcacaa
    14281 tgtcgatatt ttccagccag atgtattgcc agatatcctg ctcggtccag ttagagagcg
    14341 ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt gtagttgtgc cacagctccg
    14401 ggcgctgatt tttcggatcc cagcgatgga agcggtcacg gaaagagtag attcgctctt
    14461 tagcgcggga tttctcttcg tcacggcgcg caccaccgaa ggcggcatca aaaccgtatt
    14521 tgttcagcgc ctgtttcagg ccttcagttt tcataatatc ggtatgtttc gcgctgccgt
    14581 gcacgaatgg attaatcccc atcgccacgc cttccgggtt tttatgcacc agcagctcgc
    14641 agccgtaggc tttcgccgta cgatcgcgga actcatacat ctcgcggaat ttccagccgg
    14701 tatcgacatg cagcaacggg aaaggcagcg tacctggata aaacgccttg cgcgccagat
    14761 gcagcatgac gctggaatct ttaccgatag agtagagcat caccggattt gagaattctg
    14821 ccgccacctc gcgaataatg tggatgcttt ccgcctccag ttgccgcagg tgagtaagtc
    14881 gtttttgatc cataaccgtt cctttgcaat accgctattt tcttgccatc agatgtttcg
    14941 actataggga gcgtaagaga acgaatgaaa ttaccaatta gaatgagtag ttccttaacg
    15001 gaataacgat ttggcaaagc taatatcaaa aagtgcttaa ggcaccggat ttcgggcgtt
    15061 taggaagatt tgaaattgtt ttagcgcagc ggcagtttca tactatggcg gtaaaaaaat
    15121 ttgcatggta tttaaggact cactatgttt tccgcattgc gccaccgtac cgctgccctg
    15181 gcgctcggcg tatgctttat tctccccgta cacgcctcgt cacctaaacc tggcgatttt
    15241 gctaataccc aggcacgaca tattgctact ttctttccgg gacgcatgac cggaactcca
    15301 gcagaaatgt tatctgccga ttatattcgc caacagtttc agcaaatggg ttatcgcagt
    15361 gatattcgga catttaatag tcggtatatt tataccgccc gcgataatcg caagaactgg
    15421 cataacgtga caggaagtac ggtgattgcc gctcatgaag gcaaagcgcc gcagcagatc
    15481 atcattatgg cgcatctgga tacttacgcc ccgctgagcg atgctgacgc agatgccaat
    15541 ctcggcggac tgacgttaca aggaatagat gataacgccg caggtttagg tgtcatgctg
    15601 gaactggcag aacgcctgaa aaatacgcct accgagtatg gtattcgatt tgtggcgacc
    15661 agcggcgaag aggaaggaaa attaggcgct gagaatttac tcaagcggat gagtgacacc
    15721 gaaaagaaaa atacgctgct ggtgattaat ctcgataact taattgttgg cgataaattg
    15781 tatttcaaca gcggtgtaaa aacccctgaa gcagtaagga aattaacgcg cgacagggcg
    15841 ctggcaattg cacgcagtca cggaatagcc gcaacgacca atccgggttt gaataaaaat
    15901 tatccgaaag gcactgggtg ttgtaatgac gcagaaatat tcgacaaagc gggcattgct
    15961 gtactttcgg tggaagcgac taactggaat cttggaaata aggatggtta tcagcaacgc
    16021 gcaaaaacac ctgccttccc ggcgggaaat agctggcatg acgtaagact ggataatcaa
    16081 caacatattg ataaggcact tccaggaaga atagaacgtc gctgccgtga cgttatgcgg
    16141 ataatgctac cgttggtgaa ggagctggcg aaggcgtctt gatgggtttg aaaatgggag
    16201 ctgggagttc taccgcaggg gcggggaatt ctaagtgata cccatcatcg catccagtgc
    16261 gtccggttta cttccgggaa aaccgcaccg tattgcgccg gtttatctcc gctggagcgg
    16321 ggaacacctc tggcgaatag aaagcaagcc aggttttgac cagtcgatcg tacatggcat
    16381 tggccgttgc cattgttggc aggccaaaaa acagcccctg agcgttaccc gcagccatca
    16441 acctgtgcgc caggataagc gccgcttctg ttttacctgc gccggtcaca tcttccagaa
    16501 taaacaactg tggtcctggc tgattgatat ccagatccag caccttttgc tgtaacggtg
    16561 tcgtattctc aataaaagga aacagcgtat taattccggt gaaaggcgcg gtttctgctt
    16621 gtggcggaaa gacggttaat gcgttttgag cctgaactaa agccttttgc cagtaatctt
    16681 caatatccat tgcctgtgcg acgcgtggaa aaaatcgcgt tgacgaccct gtccagtctg
    16741 caagtacgac ggttgcagag atataccagg aaagttgttt tataagttca acgccctcgt
    16801 catcatccca gaatgtgggg atctctattc gcggaaacag tggcttgatt tcaaggagaa
    16861 aatctcgtgc tgcagctttg tcttcaggca gaaaattatc caattcatcc atacgatcag
    16921 ggggccgacc atgatgccct gtcgtcacgg gcatccacat ttctatcact cgcctaagtt
    16981 tgcgcggaga gagcgaagat gaaggaagta actcctgaca ttcacttaaa taatgattcc
    17041 acaaccagta gcccagcgtt gaatgagaga ttttttcgta attttttctg gcaccttcct
    17101 gaatcttgaa ttcagggggc aggtaaagtt gttgaaaaga gcgggcaaat tttccaatat
    17161 cgtgccagca cagcaaccag gcgaaaaatt gagctgcctg ttccttatca gaaatcccta
    17221 attgacgaaa ataatcagcc agcccgaagc aatttctttt aaccattaaa tagcccattg
    17281 cggccacatc cagcaaatgc cagcaaagaa ggtgatagtc gtcgctaccc tctttctcgc
    17341 cacgtcggtt ttttccccag aaatcaaaga aagtcacaat atttttatcc ttcagtaaac
    17401 ttaaaggata tttacgcata ttttaaaaac tatctgtgat atatatcagc caataaaaca
    17461 tttacattat gaataaatta atgattttca tttgaaattc ataataataa atagagacta
    17521 tatattcaca taaaaaatat aaattttaaa aaagggtgca ggagaccaaa ccatcacaat
    17581 tcagtacgcg gggttttacc cccgcgtggt ggctacttct tcgattcgta agcgagaact
    17641 gccttaaact ccataccacg ttctctgata ctcaactcac ataacgagtc acctaccatc
    17701 aacgtgaatc ccagtactgt acaacacagt acgatgattg ccacaagggc atattttgtc
    17761 agcatgtctt gcctccctgc aaagaagaga ctaacatccg aattgctacg ttcgaacgcg
    17821 agcctcaggt tgatagaaat atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc
    17881 ttaacgccag acagcctcaa gcacccaacg ccattctata cctgttaatt ttccctgcga
    17941 tgttcacctt taaatgccgg aatgctgctc gcaaaataaa aacgccccca tcatttctga
    18001 cagaggcgtt taatttatcc ggcaatattt acccttcgtg taatccacat tcccttttta
    18061 agccaaagaa acgcgtttct tcttccgcca tgccgggttc ccatttacgg gttgtatggg
    18121 tatcgcccac cgataaatat ccttcgtccc ataatgggtg atatttcagg ccatgttttt
    18181 gcaggtactg ataaatagtt cggttatccc agtcgataat cggcagcact ttaaatacgc
    18241 cacgctgaat tgccagcacc ggtaaattgg cacggctgcc ggattgttca cgccgcaggc
    18301 cagcaaacca cgtttgcgca ttcagttctt tcagcgcccg gttcatcggt tcgactttgt
    18361 tgatgtcatt gtacttttca atgccttcaa cgccctgttc ccacagtttg ccgtagcgtg
    18421 cttcctgcca ggcggcgctt tcggtagcgc ggtacacttt caggttgagc ttgagtttgt
    18481 ccgttaactc gtcaataaag cggtaggttt ccgggaacag atagccggtg tcggtgagga
    18541 tcaccggaat atccgggtga atctgattca ccagatgcag gctcaccgcc gcctgaatgc
    18601 caaagctgga agaaagtaca tattcaccgg gcagattatc cagcgcccag gcaacgcggc
    18661 cttcagcgtc cagtttttcc agttcggcgt tagtttctgc cagcgccaga atgcgatcta
    18721 ccttcggcag ttcgttcagg gcgtttagat cgagtttgga catagattcc tcactgtttg
    18781 ccttgcctga tgcgacgctc acgcgtctta tcaggcctac aagaccgggc tgatggttaa
    18841 tcccacaaat cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag
    18901 tcgccgaagc cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc
    18961 gacgccagga tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca
    19021 atgcggttgc cgccaagatg caggttgtag cgacccggtg ctttacccac caggcccact
    19081 tccgccagca tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc
    19141 tcatcgctga caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg
    19201 aaacgctccg cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag
    19261 ttttcacgct gcggcgtgac ggcattcatt aacccgctct ctttggcaat tttctcgatc
    19321 ttcgctttct cgctttccgg cacaccggcg atgatcagat tctggttcgc cgtaatgcgg
    19381 aaatcacctt tgtggatctt cgcgatctcc agcaggccag ttttcagcgg acgccccgga
    19441 taatcaagga tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc
    19501 ttaacccagc caatacgatc gccgcgtcct gtaaattcat acgggcggat cggttcaaat
    19561 ttgatccccg cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagt
    19621 gtgtattttg ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc
    19681 acgacggctt ccgccaccgc tagcgtatgc tctagcggca gatagccaaa ctcactcgcc
    19741 gtgcgggcgt aggttttctt gttaccgtgt tcgatagaaa gcccaccgcc caccagcagg
    19801 ttgaagccca ccagcttgcc gttttccgcg atcgccacga agttcatgtc gttggcgtgc
    19861 agatcaatat cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag
    19921 gtctgaccaa ggatcggttc ttcatcggtg gtggcgactt tttcctgatc gagccagatc
    19981 tccgcatacg cgcgggtacg cggcagcaga tgttcagaaa tcttcttcgc ccactcgtac
    20041 gcttctgcgt gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg
    20101 tcgttagcag tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg
    20161 acgttctttt tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg
    20221 ccatagatgg tgttttcacc ggcaaattta tcgatcgcct gccactgttt ggtggtgata
    20281 actccacccg gcagacggca gcgcaacagc atcgcgtggc gcggctccag cttctgttca
    20341 gcacgttcgg cgcggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc
    20401 aggaagttat cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg
    20461 ccgcgcaggt agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc
    20521 actaaaggcc ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta
    20581 cgcgcagctc acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa
    20641 tcacttccag aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat
    20701 aaatgtgggc accgtcattg atccagcgcc acagctccgc gccctgttcg cgcagtttat
    20761 cttgtacgta aactttttct ttttggtcgc gcgaccaggc gagatcgatc cgcgtcagca
    20821 caccctcttt gacgtagcgc tgccactcca cctgatacag gaagtcttcc gtaaaatgcg
    20881 gattgccaaa gaacagccag tttttacctg gcgcttcgtc agcggcgcgt tgctgcataa
    20941 aggcgcggaa cggcgcaata ccggtgcctg ggccaatcat aatcaccggg gtttccaggt
    21001 tagcgggcag gcggaagtta tcgttatgtt cgataaatac gcggacttcg ccctcttctt
    21061 ccacgcggtc agcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt
    21121 aacgcaccac accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcaa
    21181 tggaatagag acgcggggtc agcgggcgca gcagattaat tagcgcttcg gcatcaagct
    21241 gtgccgggga gaaacgcacc atgtcaacaa tcagcgtcgt cgcggcgtaa tgctgtaact
    21301 tcgctttatc gcccaccagc ggcagcagtg tttcactgcg ggtaagcgtg gcgtaattct
    21361 caacaatgtt ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag
    21421 gcaacgtttt gccctcgaca gtgacaggtt catcgcctgt cagccacagc agttcgacaa
    21481 gttctttcac cagtgccgga tcgttctgat accagacgcc cagcgcgtca cccggctggt
    21541 aacgcaggcc cgagtcgcct aagtcaattt cgatatggcg aacgtctttt tcagagttac
    21601 gcccagtaat tttctggtta acagagaggc tagccaccag cggcgcgtct ttgctgtacg
    21661 ggctggtgtg gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag
    21721 gcgcacgcga tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt
    21781 attcaacatc agaatcgaca cggtcgagca ggcgttcacc acccagttcc gccagcttgc
    21841 tgtcaaaatc tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca
    21901 cggcaaacgc ggtgttttcc agctttggcg cttttttgga gaacaggaac ttatgcagcg
    21961 cgacggcttc ttccggcggt tccccttccc cttgcgttga cgtcactacg atgagcagtt
    22021 tttcgctggc gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt
    22081 ttgctgctaa taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg
    22141 aagcagagat aatcgttata cccggcattt ctgcggcggg cgctggcgtc actgcaagcg
    22201 caacaggctg ctggttgagc acgccccaga aatagccaga aacccaggca agctgggtag
    22261 gcgttaaatc ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca
    22321 acgcggaagg tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt
    22381 ctgcgccctg tcagcgccca taaaacagaa gagaaggtaa ggttaacggg gtgaacggtg
    22441 tggattaaag acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata
    22501 gtctttaaca acaaaataga ttaaccaacc taatgaaaaa caaatgaatt tagccaatca
    22561 ttaagataaa tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc
    22621 tttccggtac tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt
    22681 atttaaagat ttcaccttcg aagccgctca ccgcttacca catgtcccgg aagggcataa
    22741 atgtggtcgc ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga
    22801 tccgcatacg ggctggatta tcgatttcgc tgaactgaaa gcggcgttta agccaactta
    22861 tgagcgcctc gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga
    22921 agttttagca aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt
    22981 gatggtaaaa gaaacctgca ccgcagggtg tatctatcgc ggcgaatgat aagagtgtgt
    23041 cggcggtcaa tttcccttta gtaacgctat gttagggtgt tgtgttctgg atatctgggg
    23101 catgacatgg aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct
    23161 tgcgcgttac gctgcgcgcg agcggattta tccgttttgt tactcgaacg cgctgaaatc
    23221 cccggcagca aaaatctttc cggcaggcgg ttatataccc atgcactcgc ggaactcctc
    23281 ccgcaatttc atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttccctg
    23341 ttaacgccgg atggcgcaac gacgttttcc agcttacagc ccggcggtga atcctggagt
    23401 gtattacgtg cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa
    23461 tgcatccccg gagcgacggt ggatgcactg tatgaagaaa acggcagagt ctgtggcgtt
    23521 atttgtggtg acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc
    23581 gtcctggctg aacgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcgttgggg
    23641 atcaaagaag tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat
    23701 aacgaaggcg cagcgttgct gttcagcggc ggaatctgtg atgacttacc cggcggcgca
    23761 tttctttata ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt
    23821 acgcaaagtc gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg
    23881 cgcacgctta tcaaaaacac ggaatcactg gagtatggtg cgcatctggt gccagaaggt
    23941 ggcttgcaca gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg
    24001 ttgcgcagtt gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc
    24061 gcacaggcgg cggcacaaac gctaataagc gcctgccagc accgcgagcc gcaaaatctg
    24121 tttgcgcttt atcatcacaa cgtagagcgc agcctgctat gggatgttct acaacgttat
    24181 cagcatgttc cggcgctttt gcaacgccct agctggtatc gggcgtggcc tgcgttaatg
    24241 caggatattt cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag
    24301 ttattctggc gtcatttacg tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg
    24361 agtctgcgat gtctgtagcc cgtaatctct ggcgcgttgc tgatgcgccg cacattgttc
    24421 cggctgactc cgttgagcgt cagatggtac aacggttgat taacgcctgt ccggtaggtc
    24481 ttttttctct cacacaggaa ggtggcttac gtattgacta tcgcggctgc ctggagtgtg
    24541 gcacctgccg tttgctgtgc gacgaatcca cactacaaca gtggcgctat ccgccttccg
    24601 gattcggcat cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac
    24661 ctgctccgcc agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggcc
    24721 atcgattctg aatgccagtt tatttccgtg ttgtacggca atatctgcac catcagtaat
    24781 atcgttaaaa agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa
    24841 ggcgcgtcaa ataaagaagt ggtgattcag tttttgaaac tggtgaccga ggcggatgga
    24901 attatcagca ccaaagcatc gatgctaaaa gcggcaagag cggaagtttt tttctgtatt
    24961 catcgcctgt ttattgttga ttcgatttcg tttcacaaca ttgataagca agttgcgcaa
    25021 tcgaatccgg attgtattga gatcctgcca ggctgcatgc ccaaagtgct gggctgggtg
    25081 acggaaaaaa tccgccaacc gctgattgct ggcgggctgg tatgcgatga agaagatgcg
    25141 cgtaatgcga ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg agtctggacg
    25201 ttagcgaaaa aattgctttg acgggataat cgtaaccaat tgaatttgtt ttgatttgtt
    25261 ggccgcacgc cacatccgac attcagcgcc tgatgcgacg cttaacgcgt cttatcaggc
    25321 ctacaggtcc ccagtccaga accgtaggca ggatcatccg gcagcacgtt attggtattc
    25381 tgcgtgaatg tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgtcaat
    25441 atctgcctgt gaaaataccg ctgcgctggc gtcatgatta atcgccacca taaacttgct
    25501 attgcgtacg cccgccatta acgctgctgc gccggaagcg ccaacaacaa tgcacacctc
    25561 aggtgccagc agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat
    25621 cactcgcgcc cgactgtagc ccacttcggt tcccagcttt tctgccagca taacgatttc
    25681 ctggttatct gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg
    25741 gtcgcggcta acatttttca gatcctctgt gctaaccagc cagtcgggaa gcgcacctgg
    25801 gacaatgttc agttgctgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg
    25861 acgcgccagg gaaaggcata acgggcgttt ttcagtttgc agcgtcgccg ttaacgcatt
    25921 tccccagtgg gatttgcgca tgcttacggt tggaatatct aacgaagtca cctgacagat
    25981 gcttgcacca tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg
    26041 cggaaagaga acaacgtccg caggcgttcg ctgccactgt tccaccatcg catccagcac
    26101 ctgttccgcc actacgggct gaggttctat ttgccagtgc gccagcgtac aaccgctgaa
    26161 atcctgcgcc gccagccagc tcgctattgc ggcattctcc tgatttattg tcacaattgc
    26221 gatattcatg gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg agctttttct
    26281 gctaccgttt gcccatcaat cagcgtcgct ccgcatcgtt gctctgctct tgccagttgc
    26341 agacactgca tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc
    26401 gcagccattc gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg
    26461 gcaatcaccg caggcaagcg aacccggcaa cagcgcaacc catgttcagt acgctgttcg
    26521 agggtgataa acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc
    26581 agcatttccg ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg
    26641 gtaattatca gatccagcgg attctgacgc tgccattcgg caatatggcg agcgacaaat
    26701 tccggcgcaa agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga
    26761 gccatgagat agcgcagcca gtgcaacgcc cgttcatccc ccatgctcaa cgcggttaaa
    26821 gacatcggcg taccgttttt tctctgcgct aacagcagcg cggcagcggc ctgttcatca
    26881 gcaccgagta aacttcgcag tagcgaaaca tccggtccgc tattaccctg agccgccgcc
    26941 tgccactctt tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg
    27001 ttcattcgca cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc
    27061 ccatagccag gtcacaacaa aaccaaccaa caacactgtc gccaggagca ataaggtgac
    27121 ttgcattccc cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag
    27181 tcgactcatg gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc
    27241 cgcaggcaaa atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa
    27301 aagcagcagc gttaatgaac tgccggaagg caaacaggcc attactacca gcgttgccgc
    27361 cagcagcaaa aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa
    27421 tcccagcagc gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc
    27481 ttccagacca atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat
    27541 tacgaggcag acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa
    27601 caaggttttg atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg
    27661 accaaaatag cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc acagtagcca
    27721 acgtggcgat tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcaaagctgc
    27781 cgatgccagc aaccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat
    27841 acttgccaac acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg
    27901 atggcggcgc ggggaaaatt cagccagcaa ggtgtgacct actgagtaat cgcctcccag
    27961 accaatgccg atcaaaatgc gcagcccaat aagatgctct ggcgtggtgg caaaaaattg
    28021 caagaacgaa gcgagcgtaa tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat
    28081 atggtcggag atccacccaa gaaccaggct gccaagaaac aaaccaagga gcgccgagcc
    28141 gccgatcatt cccgccataa acggcgtcag ttgcatggcg ggcgtaagtt gaataatggc
    28201 gtaaccaatg acgccgagaa cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc
    28261 aattcgacag tgaaaacggt taagcggtaa atcatccatt cgcaccggtg aagtgttcat
    28321 ctttacccct tcattgtccg gtaataagaa cggcttctcg cctgagaagc cgaaaaggca
    28381 acgccccgtc atcgcggaac gctgcctgat acattatttt tcaatcggat agatagtacc
    28441 cgtattcata atgccgttag gatcgaactg ctttttcagc ccttccagca acgcccacgc
    28501 gctgccgtgt tccagcttgc tccagtgaac gcgatgttta ccaatgccat ggtggtgcac
    28561 catcgaaccg ccgaggcgaa tggtttcttc gcagatgatt ttgttgagcg gattgtagta
    28621 cttgtcgatt tcctcttccg gcttacagtc aacaacgttg tagtcataga cgaagtacat
    28681 gttggtgccg ttctgatagc tatgagagga gtgaccgccc agcatggtga tgtcgtcggc
    28741 gtgcgggaac tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct
    28801 ccagcagccg gacacttcgg tggtaaagcc catgttgccg gttttgagga tctgcacacg
    28861 ttcggcagcc actttatccg gtccccagtt caggttgtta aaccaggttt caatcagttt
    28921 gctgtccacg cgctggcact gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt
    28981 cgccttcgca atgcgagggt taccttcagc cataaagatc agcacccatt ttccgtcggc
    29041 aaaatgggtg aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg
    29101 atacccttcc accatgattt cacgcaggat gttgaagccg gttttcatgt cttccaggat
    29161 atagccgtag aagaggttgt tttccggggt gtatttaaag attttcactg ttacttcagt
    29221 gatatagcac aatgcacctt cgttgccgat gatgatgtga cgaatgtccg ggccagccgc
    29281 gcggcgtggc acgtttttaa tgcgtgtgac ggtgccatct gccaacactg cctccagacc
    29341 aacgaccata tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc
    29401 taccaggccg cccatctgcg ccagcggctt tgactgcgga gaatgccccg tggtgtaacc
    29461 tttttcacgc aacgcgtttt ccagcacttc cagcggaaca ccacattgca ccgtcgcctg
    29521 catattctca atatcaatat taatgatttg attcatggcg gagccgtcga gcaccaccga
    29581 gttttctaca acagtttcca gcccaccttc ggtggcggaa gcaccggtac gcggcacacc
    29641 gttaattttg tgcgcattca taaaattcag cacacgggat acttactctg tggaaccgag
    29701 ttttacgacc gctgccggaa tcggcaaagt ataaatgcca tgaatatccg gaaatttacg
    29761 aaaacggtca atactgtttt tctttaatac tgtttcatcg gtaattacgc gatctgcacc
    29821 aacaatttcc tttagctggt cgacaatcgc tgcgcgagat aaagacatag taattccttc
    29881 ctgataataa aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta
    29941 gcgcactaaa taaccgccat cgaccactaa taaatgcccg ttgacataat tcgatgcctg
    30001 acttgcgaga aacacggctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg
    30061 aatatgatca agaacgcgct gattggtttc tggattactg cgtgtcgcca gcataatatg
    30121 ctgttgcata atagccaggg gcgataccat ttacctgaat attatattga cctagttcat
    30181 cacaataagc tttggtgaac ccggcaagag catgtttagt ggcagaatat gcaggtgacc
    30241 attgtccacc taagtaagag aacaatgaac agatattaat aattttgccg cttttctgcg
    30301 ggatcataat ttttgccgct tcatagctta actcgaatgc ggcggtcagg ttcacatcaa
    30361 tcatcggatc ccagtcggca cgaccgaagt ccagcacctt attcagctta caaataccgg
    30421 cattgttaac cagaatatcg acagtaccga agcgctcaca gcaggcagcg ataatcttct
    30481 gcggtgcgcc ttctgcggta atatccacct gcatgaagtc cacttcaaca ccctgttttt
    30541 caatcatttc ctttgtttcg ccgttatctt tgacgaagct aggaataaaa atatttgcgc
    30601 cagctttggc caacgccatt gcaaatgcct gacctaaacc gctattcccg ccggtaacaa
    30661 ttgctgtttt acctttcagg gagaaaaaat ccattgagaa cgcattgaga gattcgattg
    30721 acatagttag ctccaatttc cgtaaggcaa aaaaaagaaa ggtaacttcc cccgcgagag
    30781 ggaagttacc tttctcatta tctctggtaa ctggagataa tttttagcat aacggcataa
    30841 ctgctaatgt gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca
    30901 gaagcgtgat tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac
    30961 tagagacctg agaaaagtaa tccacctgaa agggcggctt acttttctct tttttttgtc
    31021 tattacaaat gggaatacga tatgcaacac aactcatatc gccgttggat aaccctcgcg
    31081 ataattagtt ttagcggcgg cgttagtttt gacctggctt atttacgtta tatttatcaa
    31141 attcccatgg cgaaatttat gggattcagc aataccgaga taggtttaat aatgagtacc
    31201 tttggtattg cggctattat tctttatgcc cccagcggcg ttattgccga taaattttca
    31261 caccgcaaaa tgattacttc cgcgatgatc attaccggat tactgggtct gttaatggca
    31321 acgtatccac cgctgtgggt aatgctctgt attcaggtcg cctttgcgat aacgacgatt
    31381 ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc ttggcgatca tagcgagcaa
    31441 gggaaaatta tgggctggat ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg
    31501 tttaccatgt gggtcttttc tcgctttgca ccggatgaca gcgccagcct gaaaacagtc
    31561 attatcatct acagtgtggt ttacatcttg ttggggattc tgtgctggtt ttttgttagc
    31621 gataacaaca acctgcgcag tgccaataac gaagaaaaac agtcattcca gcttagcgac
    31681 atccttgccg ttttgcgtat cagcaccacc tggtattgca gcatggtgat ttttggtgtc
    31741 ttcacaatct acgccattct gagttactcc accaactatc tgaccgaaat gtacggcatg
    31801 tcgctggtgg cagcgagcta catggggatt gtgatcaaca aaatcttccg cgcgctgtgc
    31861 ggcccacttg gaggcattat caccacctac agtaaagtga aatcccctac ccgcgtgatt
    31921 caaatccttt ccatcatcgg tttgctggcg ttgacagccc tgctcgtcac gaactctaac
    31981 ccgcaatcgg tcgcgatggg gattggcctg attttactgc tgggattcac ctgttacgcc
    32041 tcacacgggc tgtactgggc ctgccccggc gaagcgagaa caccgtctta cattatgggc
    32101 accacggtag gtatttgttc ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt
    32161 atcggccact ggcaagacac cctgcccgct gcagaagcct accgcaatat gtggctgatg
    32221 ggcatggcgg cgcttgccat ggtgatagtc tttacctttt tgctgttcca aaaaattcgt
    32281 actgctgata gcgcccccgc aatggctagc agcaagtaag ccaggaaaat tacaggagaa
    32341 aaatcatgtc gaaaaaatac atcataggga ttgatggcgg aagtcagagc acaaaagtgg
    32401 tgatatacga tctggaaggt aatgtggttt gcgaagggaa aggcttattg cagccgatgc
    32461 acacgccaga tgccgatact gcagaacatc ctgacgacga tttatgggca tcattatgtt
    32521 ttgccggtca cgatttgatg agtcagtttg ccgggaataa agaagatatt gtcggtattg
    32581 gtctgggatc catccgttgc tgccgtgcgt tattgaaagc cgatggcacg cccgctgcgc
    32641 cgttgattag ctggcaggat gcacgcgtaa cgcgtcccta cgaacacact aatcctgacg
    32701 tggcatatgt cacctctttt tcgggttatc tgacgcatcg cttaaccggc gagtttaaag
    32761 acaatatcgc caactatttt ggtcagtggc cggtggatta taagagctgg gcatggagcg
    32821 aagatgctgc ggtaatggag aagtttaata tcccccgtca tatactgttt gatgtgcaaa
    32881 tgccaggcac tgttcttggg catatcacac cacaaggcgc gctggcgaca catttcccag
    32941 ctgggctgcc ggttgtttgt accaccagtg ataaaccggt agaagctctg ggggccggat
    33001 tactggatga tgaaacggcg gtgatttcac tcggtactta tatcgcgctg atgatgaacg
    33061 gcaaagcctt gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa
    33121 cattgctgta tgaaggttac ggtattcgca aaggtatgtg gacggtgagc tggctgcgcg
    33181 acatgttagg cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt
    33241 tactcaacaa gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg
    33301 attggctgac caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca
    33361 gcatggatta cgcatggata tatcgttcga tactggaaag cgtggcgctg acgctgaaga
    33421 acaattacga caatatgtgt aatgaaatga atcactttgc gaagcatgtg atcattactg
    33481 gcggcggttc gaacagcgat ctgtttatgc agatttttgc cgacgtgttc aaccttccgg
    33541 cacgacgtaa cgccattaac ggttgtgcaa gcctgggggc agcgattaat acagcggtag
    33601 gtctggggct atacccggat tacgcaacgg ctgtcgacaa aatggttcgc gtgaaagata
    33661 tctttatgcc tgttgagagc aatgccaaac gctacgacgc gatgaataaa ggtattttca
    33721 aagacctaac caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg
    33781 aattggggaa tgcggattcg atccagagct ggtcgaatgc gtaagtgagg aagaccgggc
    33841 gggaaactgc ccggcctgaa tatacctgaa tggttatccc cgctgacgcg gggaactcga
    33901 ataactgacc aacgtctggc tctctaaatc cggtttatcc ccgctggcgc ggggaacact
    33961 ctaaacataa cctattatta attaatgatt ttttaagcca gtcacaatct accaacttta
    34021 caggatcaca caaacaacac atccattatg ttaaagagca cttaatccat tgattaaaaa
    34081 ggtaaatatt taaaataact ctatacaaac taaaatctac caaacttcgc cgcaataatt
    34141 ttcactccag caaaaaatta acgccgcaga atttgtagag aatgataatg attagtccgg
    34201 gatgggcaag atggatgagt tttcaggggc gacaaataat acaaatgagt ggaatttttt
    34261 acaagttcca cccatttata a
//
LOCUS       DADLXX010000030        33542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLXX010000030 DADLXX010000000
VERSION     DADLXX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.030"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="JNX27_001443"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="JNX27_001443"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7882696.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /locus_tag="JNX27_001444"
     CDS             complement(2999..3928)
                     /locus_tag="JNX27_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7882697.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="JNX27_001445"
     CDS             complement(4145..4381)
                     /locus_tag="JNX27_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7882698.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="JNX27_001446"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="JNX27_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7882699.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYNAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="JNX27_001447"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="JNX27_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7882700.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRK
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="JNX27_001448"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="JNX27_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7882701.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSI
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSL
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="JNX27_001449"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="JNX27_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7882702.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="JNX27_001450"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="JNX27_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7882703.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB"
                     /locus_tag="JNX27_001451"
     CDS             complement(10727..11353)
                     /gene="ipgB"
                     /locus_tag="JNX27_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7882704.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="JNX27_001452"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="JNX27_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7882705.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="JNX27_001453"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="JNX27_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7882706.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="JNX27_001454"
     CDS             13569..15185
                     /locus_tag="JNX27_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7882707.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVYYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="JNX27_001455"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="JNX27_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7882708.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="JNX27_001456"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="JNX27_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7882709.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17130
                     /gene="mxiG"
                     /locus_tag="JNX27_001457"
     CDS             16024..17130
                     /gene="mxiG"
                     /locus_tag="JNX27_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7882710.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQSNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFFR"
     gene            17148..17399
                     /gene="mxiH"
                     /locus_tag="JNX27_001458"
     CDS             17148..17399
                     /gene="mxiH"
                     /locus_tag="JNX27_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY7882711.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17412..17705
                     /gene="mxiI"
                     /locus_tag="JNX27_001459"
     CDS             17412..17705
                     /gene="mxiI"
                     /locus_tag="JNX27_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7882712.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17711..18436
                     /locus_tag="JNX27_001460"
     CDS             17711..18436
                     /locus_tag="JNX27_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7882713.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18433..18960
                     /locus_tag="JNX27_001461"
     CDS             18433..18960
                     /locus_tag="JNX27_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7882714.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18902..19597
                     /locus_tag="JNX27_001462"
     CDS             18902..19597
                     /locus_tag="JNX27_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7882715.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19590..19997
                     /locus_tag="JNX27_001463"
     CDS             19590..19997
                     /locus_tag="JNX27_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7882716.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19981..20409
                     /gene="mxiM"
                     /locus_tag="JNX27_001464"
     CDS             19981..20409
                     /gene="mxiM"
                     /locus_tag="JNX27_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7882717.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20539..21171
                     /gene="mxiE"
                     /locus_tag="JNX27_001465"
     CDS             20539..21171
                     /gene="mxiE"
                     /locus_tag="JNX27_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7882718.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21158..22858
                     /gene="mxiD"
                     /locus_tag="JNX27_001466"
     CDS             21158..22858
                     /gene="mxiD"
                     /locus_tag="JNX27_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7882719.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22877..23944
                     /gene="mxiC"
                     /locus_tag="JNX27_001467"
     CDS             22877..23944
                     /gene="mxiC"
                     /locus_tag="JNX27_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7882720.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23957..26017
                     /gene="mxiA"
                     /locus_tag="JNX27_001468"
                     /gene_synonym="virH"
     CDS             23957..26017
                     /gene="mxiA"
                     /locus_tag="JNX27_001468"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7882721.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26030..26431
                     /gene="spaK"
                     /locus_tag="JNX27_001469"
     CDS             26030..26431
                     /gene="spaK"
                     /locus_tag="JNX27_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7882722.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26435..27727
                     /locus_tag="JNX27_001470"
     CDS             26435..27727
                     /locus_tag="JNX27_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7882723.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27820..28158
                     /gene="spaM"
                     /locus_tag="JNX27_001471"
     CDS             27820..28158
                     /gene="spaM"
                     /locus_tag="JNX27_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7882724.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28145..29023
                     /gene="spa32"
                     /locus_tag="JNX27_001472"
     CDS             28145..29023
                     /gene="spa32"
                     /locus_tag="JNX27_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7882725.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29017..29898
                     /gene="spaO"
                     /locus_tag="JNX27_001473"
     CDS             29017..29898
                     /gene="spaO"
                     /locus_tag="JNX27_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7882726.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29903..30553
                     /gene="spaP"
                     /locus_tag="JNX27_001474"
     CDS             29903..30553
                     /gene="spaP"
                     /locus_tag="JNX27_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7882727.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30568..30828
                     /gene="spaQ"
                     /locus_tag="JNX27_001475"
     CDS             30568..30828
                     /gene="spaQ"
                     /locus_tag="JNX27_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7882728.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30828..31598
                     /gene="spaR"
                     /locus_tag="JNX27_001476"
     CDS             30828..31598
                     /gene="spaR"
                     /locus_tag="JNX27_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7882729.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31605..32633
                     /gene="spaS"
                     /locus_tag="JNX27_001477"
     CDS             31605..32633
                     /gene="spaS"
                     /locus_tag="JNX27_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7882730.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32646..32888
                     /locus_tag="JNX27_001478"
     CDS             32646..32888
                     /locus_tag="JNX27_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882731.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10474 a         4876 c         6709 g        11483 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccctgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc tggtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagttact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg tggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca agtcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgcgttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagagggggg gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatatccttt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgatgtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtagtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgtttttttg
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa atactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtaa gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttg tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttgttagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgt
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttatta atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttatt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggga gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgtc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ctaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt ttttagataa aaataagtga ggataaaatg agtgttacag
    17161 taccgaatga tgattggaca ttgagttcat tatctgaaac ttttgatgat ggaactcaaa
    17221 cattacaagg tgaactaaca ttggcactag ataaattagc tagaaatcct tcgaatccac
    17281 agttgctggc tgaataccaa agtaaattat ctgaatatac attatatagg aacgcgcaat
    17341 ccaatacagt gaaagtgatt aaggatgttg atgctgcaat tattcaaaac ttcagataat
    17401 agggagcatt catgaattac atttatccag tcaatcaggt tgatattatc aaagccagtg
    17461 attttcaatc tcaagagata tcaagtctgg aagacgtcgt gtcggctaaa tatagtgata
    17521 ttaagatgga tacagatatt caagtatcac aaataatgga gatggtaagc aatccagaat
    17581 cattaaaccc agaatctttg gccaagttac agacgacgct ctcaaattat tcaataggag
    17641 tatcattagc tggcacgtta gcaagaaaaa cagtttcggc tgttgaaact ttattaaagt
    17701 cttaatttat atgattaggt ataaaggttt tattttattc ttgttgctga tgttgattgg
    17761 atgtgagcaa cgtgaagagt taatttctaa tttatctcaa agacaggcaa atgaaataat
    17821 atctgtgcta gaacgccata atattactgc tagaaaagtt gatggaggta aacaggggat
    17881 ctcggtacaa gtcgaaaagg ggacatttgc atcggcagtt gatttgatgc gcatgtacga
    17941 tttgccaaat ccggagagag ttgatatctc acaaatgttt cctacagatt cattagtgtc
    18001 ttctccaaga gctgaaaagg cccgtttata tagtgctatt gagcaacggc tggaacagtc
    18061 tttagtttct attggtggtg ttatttcggc aaaaatacat gttagctatg atcttgaaga
    18121 aaaaaatata tcttcaaaac cgatgcatat atcagtaatc gctatatatg actcaccgaa
    18181 agagtctgaa ctattagtta gtaatattaa gcgatttttg aaaaacacct tttctgatgt
    18241 taagtatgaa aatatatctg tcatattaac tccgaaagaa gaatatgttt atacaaatgt
    18301 acaacctgtt aaggaagtta aatcggaatt tttaacaaat gaagtaatat atttatttct
    18361 cgggatggct gtactagttg tcattctttt ggtatgggca ttcaaaacag ggtggttcaa
    18421 gagaaacaaa atatgataag aatggatgga atttataaaa aatatctttc aataattttt
    18481 gatccagcgt tctatataaa tagaaatcgg ttgaatttgc cttctgaact gttagaaaat
    18541 ggcgtaatca gaagtgagat taataatctc ataattaata aatatgatct aaattgcgat
    18601 attgaacctt taagcggggt aaccgctatg tttgttgcca actggaattt acttccagct
    18661 gttgcttatt ttatcggtag tcaagagtct cggttaatta atcatagtga gatggttatt
    18721 agttattatg ggggcaaaat cagtaagcaa ggggaggccg ctattcgaag tggtttttgg
    18781 catcttattg cgtggaagga aaatatatct gttggtatat atgaaagaat aaacttatta
    18841 tttaatccaa ttgctttgga gggaaattat acgcctgtag aaagaaattt gtcccgatta
    18901 aatgaaggta tgcaatatgc aaaaaggcac tttaccggta tccagacatc atgcctatga
    18961 tggtgttgtt ataaagagaa ttgaaaagga attatgtaaa accataaaag atagagacac
    19021 tgagagtaag aagaaagcaa tttgtgttat aaaggaggct acgaaaaaag ctgaatcact
    19081 gcgtatagat gctgtatgtg atggttatca aattggtata cagactgctt ttgagcatat
    19141 tattgactat atttgtgaat ggaagttaaa gcaaaatgaa aacagaagaa atattgagga
    19201 ctatataact agtttgcttt cagaaaattt acatgatgag aggatcatta gcactttatt
    19261 ggagcagtgg cttagttcgt tgcgtaatac agtaacagaa ttaaaagtgg ttttgcccaa
    19321 gtgtaattta gcactgagta aaaagttaga acttgatctc cataaatatc gctcagatgt
    19381 caagattatt ttaaaatatt ctgaagggaa taattatata ttctgtagtg gtaatcaagt
    19441 agtggagttt agtcctcagg atgtaatatc aggtgttaaa atagaacttg cggaaaaatt
    19501 aacaaaaaat gataaaaaat atttcaaaga attagcacat aagaaactga gacaaattgc
    19561 agaagacttg ttaaaagaga atcctgttaa tgattaatca aataaatgca agcaatgctc
    19621 tgcagcaaag attaaatagt gaagaatttg ttaatttaaa tgagagatta tcatcttcgc
    19681 aatcatttga tgaggatata atctatgaaa taatgcaata tttctcacag agtgaattaa
    19741 attccataga taatgatgaa ctgcacaata agattgaaca attattcaat tcacgttttc
    19801 cttatcttac agctgcacaa aaaagttcac ttcttaataa actcatagat gcaaatcaat
    19861 atgtcgattt gcatgaggga ttttatgcat cattgtccat atataacaat attgatttct
    19921 atattaagac tacaaccttc gattcattaa ttagtgtctt tgaagcaggg agagaggcag
    19981 atgattcgac atggtagtaa taagttgaaa atatttattt taagtatatt gctattaaca
    20041 ctgagtgggt gtgctttaaa gtcatcatct aattctgaaa aagaatggca tattgttcct
    20101 gtaagtaagg attatttttc tattccaaat gatttattat ggtcgtttaa tacaaccaat
    20161 aaaagtataa atgtttactc taaatgtatt agtggtaagg cggtttatag ttttaatgca
    20221 ggtaaattca tgggcaactt taatgttaag gaagtagatg ggtgcttcat ggatgcacaa
    20281 aagatagcta tagataaact attttctatg ctgaaagacg gggttgtttt aaaaggtaat
    20341 aagataaatg ataccatcct tatagagaag gatggggaag ttaaattaaa attaattcga
    20401 gggatataat tgtattgtga gtaaatataa aggtctaaat acaagtaata tgttttacat
    20461 ttactctagt ggacatgaac cagttaacgt tgagcttgta aaagataaag aacgtaacat
    20521 aattgagctg gctccagcat ggaagggttt ttttttgtcc gaaatcaaaa tataaaattc
    20581 agtgataatg tgaactatca ttatcgcttt aacattaata gttgtgcaaa atttcttgct
    20641 ttttgggact atttcagtgg tgctctagta gagcatagcc atgctgaaaa atgtattcat
    20701 ttctatcatg agaatgatct tcgggatagt tgtaataccg aatcgatgct tgataagtta
    20761 atgttaagat tcatcttttc atctgaccag aatgtaagta atgcactggc tatgatacga
    20821 atgactgagt catatcattt ggttctttat cttctgagaa ctattgagaa agaaaaggag
    20881 gtaagaataa agagtctgac agagcattat ggtgtgtcgg aggcttattt tagatcttta
    20941 tgtagaaaag ctttaggggc aaaagtcaaa gaacaactga acacttggcg gttagtaaat
    21001 ggtttattag atgtattttt gcacaatcaa acaatcactt ctgctgccat gaataatggg
    21061 tatgcatcaa catcccattt ttcaaatgag ataaaaacac gactaggctt ttctgcaaga
    21121 gaattatcga atattacttt tttagtgaaa aaaataaatg aaaaaattta atattaaatc
    21181 tttgactctc ttgattgtat tgttaccatt gatagttaat gctaataata ttgactctca
    21241 tttattagaa caaaatgaca tagcgaaata tgttgctcaa agtgatacgg taggatcttt
    21301 ttttgaacgt ttttctgcgt tattaaatta tcctattgtt gttagcaaac aagctgcaaa
    21361 aaagagaata tctggggagt ttgatttatc taatccagaa gaaatgcttg aaaaactgac
    21421 attacttgtg ggactcatat ggtataaaga tggaaatgca ttatacatat atgactctgg
    21481 agaattaata agcaaagtta ttttgctgga gaatatatca cttaattatc ttattcagta
    21541 tctgaaggat gctaaccttt atgatcacag atatcctata agaggaaaca ttagtgataa
    21601 aacattctat atatctggtc ctccggcatt ggttgaactg gtagctaata cagcaacttt
    21661 gcttgataag caggtttctt ctattgggac agataaagtt aattttgggg ttataaaatt
    21721 aaaaaataca tttgttagtg acagaactta taatatgaga ggggaggata ttgtcatccc
    21781 aggggtggct actgttgtag aacgtttatt aaataatggt aaggctctga gtaacagaca
    21841 agcccaaaat gatccgatgc caccctttaa tattacacag aaagtatctg aggatagtaa
    21901 tgacttttca tttagttcag ttaccaatag ttctatatta gaggatgtat cacttattgc
    21961 atatccagaa accaattcta tattagtcaa aggtaatgat cagcaaatac agattattcg
    22021 cgatattatc actcagttag atgtagccaa acgacatata gaattatcat tatggataat
    22081 cgatattgat aaatctgaat taaataacct tggtgttaat tggcaaggaa cggcttcatt
    22141 tggtgattcg tttggtgcgt catttaatat gagctcatca gcatcgataa gcactttgga
    22201 cggcaataag tttattgctt cagttatggc attaaatcag aaaaagaaag caaatgtagt
    22261 ttcaaggccg gtgattttga cacaagagaa tattccggct atatttgata ataacaggac
    22321 tttttatgtg tcattagttg gtgagcgcaa ttcttcgttg gagcatgtta cgtatggaac
    22381 attgataaat gttatacctc gcttttcatc tcggggacaa attgagatgt ctttaacgat
    22441 agaggatgga acaggaaata gtcagtcaaa ttacaattat aataatgaaa atacatctgt
    22501 ccttccagaa gttggaagga ccaaaatcag tacaatagcg agagttccac aaggcaaaag
    22561 tttgttgata ggtggttata cgcatgaaac gaactctaac gaaatagtat ctattccttt
    22621 tctctcctct attcctgtaa tcggtaatgt gttcaagtat aaaacaagta atatcagtaa
    22681 tattgtaagg gtttttctga ttcaaccccg tgagataaag gagtcaagtt attataatac
    22741 tgcagaatat aaaagcctca tatctgaaag agaaatacaa aagactacac aaataatccc
    22801 atctgaaact actttgctgg aagacgaaaa atcattggtt tcatacttaa attactaact
    22861 ataaagtagg tgatgtatgc ttgatgttaa aaatacagga gtttttagct ctgcattcat
    22921 tgataagttg aatgcaatga caaattcaga tgatggagat gagactgctg atgcagagct
    22981 tgattctggc ttggctaata gcaagtatat tgactcatct gatgagatgg cttccgctct
    23041 ttcgtcattt ataaacagaa gagaccttga gaaactgaaa ggaacaaata gtgatagtca
    23101 ggaacgtatt ttagatgggg aagaagatga aattaatcac aagatttttg atttaaagag
    23161 aacgttaaaa gataaccttc ctctagatcg ggattttata gacagactaa agagatattt
    23221 taaagatcca agtgatcaag tcttagcatt aagggaactt ttgaatgaaa aagatcttac
    23281 tgctgaacaa gtcgaattat taactaaaat tattaatgag ataatatcag gtagtgaaaa
    23341 aagtgttaat gctggaataa attcagctat acaggctaaa ttatttggca acaaaatgaa
    23401 acttgaacca cagcttttgc gtgcatgtta tcgtggtttt atcatgggga acatatcaac
    23461 aacagatcag tatatagaat ggcttggtaa ttttggtttt aatcacagac atacaattgt
    23521 gaattttgta gagcagtcac tgattgtaga catggattct gagaaaccga gctgtaatgc
    23581 ttatgagttt ggttttgtgt tatctaaatt aattgcaatt aagatgatta gaacttcaga
    23641 cgtaattttt atgaagaaac tggaatcctc aagcttgcta aaagatggca gtttaagtgc
    23701 agagcagcta ttgctaactt tattatatat ttttcaatat ccaagtgaaa gtgagcaaat
    23761 tcttacttct gttatagaag tatcacaagc cagtcatgag gattctgtag tgtatcaaac
    23821 atatctatct tctgttaatg aaagtcctca tgatatattt aaaagtgaaa gtgaaagaga
    23881 aattgcgatc aatattctac gagagcttgt cacaagtgca tacaagaaag agctttctag
    23941 ataacaggag ataaaagtga tccagtcttt tcttaagcaa gtaagtacta agcctgaatt
    24001 aattatatta gtgcttatgg ttatgatcat tgctatgttg atcataccat tacctacata
    24061 ccttgttgat tttttaatag ggcttaatat tgtacttgct attctcgttt ttatgggatc
    24121 attttatatt gaaagaatat tgagtttttc tacattccct tcggttttgt tgataacgac
    24181 attatttcgt ctcgctttat caataagtac tagtcgacta attttagttg atgcggatgc
    24241 gggtaaaatt attactacat ttggccagtt tgtcattggt gatagtctgg ctgtaggttt
    24301 tgttattttt tcaatagtaa ctgttgtgca gttcattgtt ataacaaaag gatctgaaag
    24361 ggttgcggag gtagctgctc gcttttctct tgacggtatg ccggggaaac aaatgagcat
    24421 tgatgcggat ttaaaggctg gaattattga tgccgcagga gctaaagaaa gacgcagtat
    24481 tctggaacgt gagagtcaat tgtatggttc atttgatgga gcaatgaagt tcattaaagg
    24541 tgatgcaatt gctggcatca ttattatttt tgtcaattta ataggaggta tttctgttgg
    24601 tatgagtcaa catggaatgt ccctatctgg tgcattatct acttatacca tactaactat
    24661 tggtgacgga ttggtctctc agattcctgc tctgttaatt tctataagtg caggatttat
    24721 cgttacaaga gttaatggtg atagcgataa tatgggacgt aacataatgt cccagatatt
    24781 tgggaatcct tttgttctca ttgttacatc agctcttgcc ttggcgatag ggatgttgcc
    24841 aggttttccg ttttttgttt ttttcctgat agcagttact ttgacggctt tattctatta
    24901 taaaaaggtc gtagaaaaag aaaaaagtct gtctgagtct gattctagtg gctatactgg
    24961 tacatttgat atcgataata gccatgactc atctttggca atgatagaaa atctggatgc
    25021 tattagttca gaaaccgttc ctttaatttt attatttgcc gaaaataaga taaatgcaaa
    25081 tgatatggaa ggtcttattg aaaggataag aagtcagttc tttattgatt atggtgtcag
    25141 gcttccaact attttatata gaacaagcaa tgagcttaag gtcgatgata ttgttttgtt
    25201 aataaatgag gtgcgtgccg atagttttaa tatatatttt gacaaagtgt gtattacaga
    25261 tgaaaatgga gatatagatg ctctaggtat tcctgttgtt tcaacttcat ataatgaacg
    25321 tgttatttct tgggtagacg tttcatatac agaaaatcta actaatattg atgctaaaat
    25381 taaaagtgct caagatgaat tttatcacca gttgtcacaa gctttattaa acaacataaa
    25441 tgagattttt ggtatacaag aaacaaaaaa tatgttagat cagtttgaaa atcggtatcc
    25501 tgatctatta aaggaagtct tccgacatgt gactatacag agaatttctg aggtattaca
    25561 aagattgctt ggagaaaata tttctgttcg caatttaaaa cttattatgg agtctttggc
    25621 gctttgggct cccagagaaa aagatgtcat aacattagtt gaacatgtcc gtgcatcact
    25681 ttctaggtat atttgtagta aaatagctgt ttctggtgag attaaagttg tgatgctttc
    25741 cggatatatt gaggatgcaa taagaaaagg gataaggcaa acctctggtg gctctttctt
    25801 gaatatggat atagaggttt cggatgaggt aatggaaact ttagcacatg ctttgagaga
    25861 attgagaaat gcaaaaaaaa atttcgttct tttggtatca gtagatatac gtaggtttgt
    25921 taaaagactt atagataaca gatttaagag tatactcgtt atatcgtatg ctgagattga
    25981 tgaagcatat accattaatg tattaaagac tatttagtga ggtttaaata tgagtaacat
    26041 taatttagtt caattagtta gagatagtct tttcacgatt ggttgtcctc catcaattat
    26101 tacagattta gacagccatt cagcgattac catttctttg gatagtatgc ctgctattaa
    26161 tatagctctt gtcaatgagc aggttatgtt atgggctaat tttgatgcac caagtgatgt
    26221 aaaacttcag tcatctgctt ataacatatt aaatttaatg ttaatgaact ttagttatag
    26281 cattaatgag ctggtagagc ttcatcgttc tgatgagtac ttacagttaa gagttgttat
    26341 taaagatgac tacgttcacg atgggattgt ttttgcagag attctgcatg agttttatca
    26401 gaggatggaa atcttaaatg aggtcttata atcaatgagc tatacaaaat tgctcactca
    26461 attatctttt cctaatagaa tctcggggcc aatcttggaa acaagtctta gcgatgtttc
    26521 gattggtgag atttgtaaca ttcaggctgg aattgaaagt aatgaaattg ttgcaagagc
    26581 tcaggttgta ggatttcatg atgaaaaaac aatattaagc ttgattggaa attctcgtgg
    26641 actttcacgg caaacgttga ttaagcccac tgcccagttt cttcatacgc aagtcggccg
    26701 tggattattg ggagcagtag tcaatccttt aggggaggtt actgataagt ttgctgttac
    26761 ggataacagt gaaattcttt atcgacctgt agataatgct cctccgctat atagtgaaag
    26821 ggctgcaatt gagaagcctt ttttaacagg tattaaggtt attgattctt tactcacgtg
    26881 tggtgaagga cagcgaatgg ggatttttgc gtcagctggt tgtggcaaaa cttttctcat
    26941 gaatatgctc attgaacata gtggtgctga tatatatgtt attgggttaa ttggtgagcg
    27001 aggtcgagag gttacagaaa cggttgatta tttgaaaaac tctgagaaaa aaagcaggtg
    27061 tgttttagta tatgcaactt cggattactc ttcggttgat cgttgtaatg ctgcatatat
    27121 agccactgct atagccgaat tttttaggac tgaaggacat aaagtagcgc tttttattga
    27181 ttcattaaca aggtatgcca gagcattacg tgatgtggcc ttagccgctg gagaatcacc
    27241 tgccagaaga ggctatccgg tttcggtttt tgatagctta cccagacttc ttgaaaggcc
    27301 aggaaagtta aaggcaggtg gctctattac tgcattttac actgttcttt tggaggatga
    27361 tgattttgct gatccattag ctgaagaggt aagatccatt ttagatggac atatatattt
    27421 gagcagaaat ctagcccaaa aaggacaatt tcctgcaatt gattccttaa aaagtataag
    27481 cagggtattt acacaggttg ttgatgaaaa acatcgtatt atggccgctg catttcggga
    27541 gctactttct gaaatagaag agctaaggac aattattgac tttggtgaat acaaaccggg
    27601 ggagaatgcc tctcaggata aaatctacaa caaaatatct gttgttgaga gttttctgaa
    27661 gcaagattat cgactcggtt ttacttatga gcagacaatg gagcttattg gtgaaacaat
    27721 tagataaggt tttaaaaatc aaagataaat atcaacggtc tgttaaatta attgaagcac
    27781 acatattaac actaaaaaaa aaactctttg tatcgagatg tggaggcatt agataaaagg
    27841 attatatact tcctgcaact ggagaatgac ttagaacctg taggcgctca gagcgttagt
    27901 cagcttttca atacaagacg taagatcgct atagtaaaaa agcatattat ccaatatcag
    27961 tctgaacgaa ttcttctaaa gggaagaatt gaagaaattc aaaaagatat tgatgaggca
    28021 aatgcttcta aaagaaaact gcttcataaa gagagtaaaa tatgtaaacg gattggcttg
    28081 attaaaagaa ataactttgc caaacagttg atacttgatg agttatcaca agaggatatg
    28141 aagtatggca ttagataata taaacctaaa ttttagtagt gacaaacaga tagaaaaatg
    28201 tgagaaacta tcttcaatag ataatattga tagtctcgtt ttgaagaaga agaggaaggt
    28261 tgaaattcct gagtactctt taattgcatc taactatttt actattgata agcactttga
    28321 acataagcat gataaaggag aaatttatag tggcattaaa aatgcgttcg aacttagaaa
    28381 cgaacgagcg acatattctg atattccaga atcaatggcc attaaagaaa atattttgat
    28441 accagatcaa gatatcaaag caagggaaaa aataaatatc ggcgatatga gggggatctt
    28501 ttcatataat aagagtggaa atgcagacaa gaacttcgaa agaagtcata cttcttctgt
    28561 aaaccctgat aatctgctag aatctgataa tagaaatggt caaattggtt taaaaaatca
    28621 tagcttgtct attgataaga atattgctga catcatttct ttactaaatg gaagtgttgc
    28681 taaatcattt gagctgcctg taatgaataa aaatactgca gacataaccc catccatgtc
    28741 attgcaagaa aaatcaatag ttgaaaatga taaaaatgtt tttcaaaaaa atagtgaaat
    28801 gacttaccac tttaaacagt ggggggctgg acattctgtt agtatttcag tggagtctgg
    28861 ttcttttgtt ctaaaaccgt cagatcaatt tgtaggaaat aaacttgact taattttgaa
    28921 acaagatgct gagggtaatt acagatttga tagcagtcaa cataataagg ggaataaaaa
    28981 taatagtaca ggatataatg aacagagtga agaagaatgc taagaattaa acattttgac
    29041 gctaacgaaa aactacagat tttatatgca aagcaactct gcgagcgttt ttcgattcag
    29101 acattcaaaa ataaatttac aggcagtgaa agtttagtca ctcttacttc cgtgtgtggg
    29161 gattgggtaa ttcgtattga tacattatct tttttgaaaa aaaaatacga ggtattttca
    29221 ggattttcta cacaagaatc tttactgcat ttatcaaaat gtgtctttat agagtcgtca
    29281 tctgtatttt cgattccaga actgtctgat aagattactt tccggatcac gaatgaaatc
    29341 cagtatgcaa ctactgggag tcatttatgc tgtttttcct cttctttagg tattatttat
    29401 tttgacaaga tgccggtatt acgtaatcaa gtttctcttg acttattgca tcatctttta
    29461 gagttttgct taggttcatc taatgtaagg ctggctactt taaaaagaat tcgcactggt
    29521 gatataatca tagttcagaa actttataat ttattattgt gtaatcaagt tattattggg
    29581 gattatattg tgaatgataa taatgaggca aaaattaatc tgtcagaaag taatggtgag
    29641 tcagaacaca cagaagtttc tttggcatta ttcaattatg atgatatcaa tgtaaaagtg
    29701 gactttattc ttttagaaaa aaatatgaca atcaatgaac taaaaatgta tgtagaaaac
    29761 gaattattca agtttcccga tgacatagtt aaacatgtaa atattaaagt aaatggttct
    29821 ttggttgggc atggggaact tgtttctatt gaggatggtt atggtatcga gattagttct
    29881 tggatggtaa aggagtaatc tcatgctgag tgacatgtcc ctcatagcta cactttcttt
    29941 ttttacgcta ttaccatttt tagttgctgc tggtacctgt tatataaagt tttctattgt
    30001 ttttgtaatg gtacgaaatg cacttggttt gcagcaggtt ccatcaaata tgactctcaa
    30061 tggtattgca ttgattatgg ctttgttcgt gatgaaaccc attattgaag ctggttatga
    30121 aaattatcta aatggccctc aaaaatttga tacaatatct gatattgtcc gtttttctga
    30181 ttctggcttg atggaatata agcaatactt gaaaaagcat acagatcttg agttagcacg
    30241 ttttttccaa aggtctgaag aagaaaatgc tgatttaaag agtgcagaga ataatgatta
    30301 ttcattgttt tccctcttgc ctgcatatgc gctaagtgaa atcaaagatg cgttcaaaat
    30361 aggtttttat ttatatttgc catttgttgt tgttgatctt gttatttcaa gcattttact
    30421 tgcacttggt atgatgatga tgagtccaat cacaatatca gtaccaataa aacttgtatt
    30481 atttgttgcg cttgatgggt ggggtatcct gtctaaggcc ttgattgaac aatatatcaa
    30541 tattcctgct tagatgatgg aggtaatgtg tctgatatag tttatatggg taataaggct
    30601 ctttatttaa tccttatctt ttccttatgg ccagtaggta tagctacggt tattggatta
    30661 tctattggtt tgttacagac agtgactcaa ctccaagagc agacacttcc ttttggtata
    30721 aagcttatag gtgtatcaat atctttgcta cttctttctg gatggtatgg tgaggtttta
    30781 ttgtcttttt gtcatgaaat aatgttttta attaagagtg gggtttgatg gacatttcaa
    30841 gctggttcga aagtattcat gtgtttttaa tactcctgaa cggcgttttt tttagattgg
    30901 ctccattatt tttctttctt ccatttttaa ataacggtat aatttctcca tctattagaa
    30961 tacctgtgat ttttcttgtt gcatcaggat taattacttc tggtaaggta gacataggtt
    31021 cttctgtttt tgaacatgtt tatttcctta tgttcaagga aataattgtt ggcctccttc
    31081 tctctttttg cttgtctctt cccttttgga tatttcatgc tgttggtagc attattgaca
    31141 accagcgtgg ggcaacgctt agtagttcaa ttgatcctgc caatggtgtt gatacgtctg
    31201 agttggcaaa atttttcaat cttttttctg cagttgtatt tctatacagt ggtggtatgg
    31261 tctttatttt agaatccata caattgtctt ataatatatg cccgttattt tctcaatgtt
    31321 ctttccgtgt ctcaaatatc ttaacatttc tgactttatt ggcaagtcag gctgttattt
    31381 tagccagtcc tgttatgata gtattgttac tatcagaagt attacttggt gtattatcga
    31441 gatttgctcc gcagatgaat gctttttccg tatcattaac tattaaaagt ttacttgcaa
    31501 tatttattat tttcatctgt tcttctacta tttacttttc taaagttcaa tttttcctcg
    31561 gtgaacataa atttttcaca aatctatttg ttagataaaa tattatggca aataaaacag
    31621 aaaagccgac acctaaaaaa ctaaaggatg ccgcaaaaaa aggacagtca tttaaattta
    31681 aggatttaac gactgttgtt attattctgg tagggacatt tactataata tcattctttt
    31741 ccttaagtga tgtaatgctc ttatacagat atgtaataat taatgacttc gaaattaatg
    31801 agggtaaata cttttttgca gtggttattg tcttttttaa gataattggc ttcccacttt
    31861 ttttctgtgt tctttcggct gtgttgccaa cattggttca aacaaagttt gttcttgcga
    31921 ctaaagctat caagattgat ttttcagtat taaaccctgt taaagggtta aaaaaaatat
    31981 ttagtataaa gacaataaaa gaatttttca aaagcattct gcttcttatt attctagcat
    32041 taacaaccta tttcttttgg attaatgacc gaaaaataat tttttctcag gtgttttcta
    32101 gtgttgatgg cttatatctt atttgggggg ggctgtttaa ggatataata ttatttttct
    32161 tggcattttc tatttttgtt attattcttg actttgtgat tgagttcatt ttatacatga
    32221 aagatatgat gatggataaa caggagataa aaagagaata tatagagcaa gagggacact
    32281 ttgagacaaa gtcgagaagg cgtgagttgc atatcgagat tctttcagag cagactaaat
    32341 ctgatatacg taattcaaaa ttagtggtaa tgaacccgac tcatattgca attggtattt
    32401 attttaatcc agaaatagcg cctgcacctt ttatttctct cattgaaact aaccagtgtg
    32461 ccttggctgt cagaaaatat gcaaatgaag ttggtatacc gactgtgcgt gatgtgaaat
    32521 tagctagaaa actatataaa acacatacaa aatatagttt tgttgatttt gaacacttgg
    32581 atgaagtcct acgtcttatt gtttggcttg agcaggttga aaacactcat taataagtaa
    32641 aggagatgtg ttatatggga gttaatttct gtaataaaat aggtattgat cagtctgaat
    32701 ttgaaataga atcttccatc attaactcca ttgctaatga ggtattgaac ccaatatctt
    32761 tccttagcaa taaggatata ataaatgttt tactcagaaa gatttcatct gaatgtgacc
    32821 tggttagaaa agacatttat cgttgtgctc tggagttggt cgttgaaaaa acacctgatg
    32881 atttataatc attcataata actcttttgc tgatataaat atttttgttg aagatattta
    32941 tgtagtcaca ggatcaattg tagaaaattg ttatgttttt gttaggaact gcactaaata
    33001 atatatgata agacagcaaa aacgactgac cataatcctt cttttattag gagttgataa
    33061 gagagactat tcatcctgta atgttaaaac tctgttatat agtattcgcg attatgcaaa
    33121 gtctgtaaat gatcatgaga tactaaccga gagtaatcgt ttattatctc attgcatttc
    33181 agatagtaac ggagctttct ttaaaagtag taaatacgtt ccccttaagt acttacgtaa
    33241 aaggagaata gcacgaaaga taccaaatga ttagcacttt gctagtaagc ttgctggtaa
    33301 actatattga ccgttgggtt tgttttgaga ctgtaattta aattgtgtaa ttgcctgttt
    33361 ttgatatgtt tactccaaca acggagacag gtaaattatg gacgaaaaga agcttaaagc
    33421 ccttgcggct gaactggcta aaggcctcaa aactgaggcc gacctcaatc agttttcccg
    33481 tatgctgacg aaatatcccg gctcttaaaa cgatagtggc tcaggctttc atttttcagc
    33541 gt
//
LOCUS       DADLXX010000031        32327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLXX010000031 DADLXX010000000
VERSION     DADLXX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.031"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNX27_001479"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNX27_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7882732.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JNX27_001480"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JNX27_001480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7882733.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JNX27_001481"
     CDS             complement(3736..4092)
                     /locus_tag="JNX27_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7882734.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JNX27_001482"
     CDS             complement(4096..4512)
                     /locus_tag="JNX27_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7882735.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JNX27_001483"
     CDS             complement(4623..5336)
                     /locus_tag="JNX27_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY7882736.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5699..6241
                     /locus_tag="JNX27_001484"
     CDS             5699..6241
                     /locus_tag="JNX27_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882737.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLIQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNX27_001485"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNX27_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7882738.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNX27_001486"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNX27_001486"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7882739.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /locus_tag="JNX27_001487"
     CDS             complement(9042..10457)
                     /locus_tag="JNX27_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7882740.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="JNX27_001488"
     CDS             10540..11523
                     /locus_tag="JNX27_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7882741.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNX27_001489"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNX27_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7882742.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNX27_001490"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNX27_001490"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7882743.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14172)
                     /locus_tag="JNX27_001491"
     CDS             complement(13465..14172)
                     /locus_tag="JNX27_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7882744.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14192..14449)
                     /locus_tag="JNX27_001492"
     CDS             complement(14192..14449)
                     /locus_tag="JNX27_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882745.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKCVFN"
     gene            14767..15282
                     /gene="zur"
                     /locus_tag="JNX27_001493"
     CDS             14767..15282
                     /gene="zur"
                     /locus_tag="JNX27_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7882746.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15324..15533)
                     /locus_tag="JNX27_001494"
     CDS             complement(15324..15533)
                     /locus_tag="JNX27_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7882747.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15649..17028)
                     /gene="dinF"
                     /locus_tag="JNX27_001495"
     CDS             complement(15649..17028)
                     /gene="dinF"
                     /locus_tag="JNX27_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7882748.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="JNX27_001496"
     CDS             complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="JNX27_001496"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7882749.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17765..18133)
                     /locus_tag="JNX27_001497"
     CDS             complement(17765..18133)
                     /locus_tag="JNX27_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7882750.1"
                     /translation="MVNNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18304..20727
                     /gene="plsB"
                     /locus_tag="JNX27_001498"
     CDS             18304..20727
                     /gene="plsB"
                     /locus_tag="JNX27_001498"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7882751.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="JNX27_001499"
     CDS             complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="JNX27_001499"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7882752.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVASALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="JNX27_001500"
     CDS             complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="JNX27_001500"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7882753.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22487..24067)
                     /locus_tag="JNX27_001501"
                     /pseudo
     CDS             complement(22487..24067)
                     /locus_tag="JNX27_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24296..25216)
                     /gene="malM"
                     /locus_tag="JNX27_001502"
     CDS             complement(24296..25216)
                     /gene="malM"
                     /locus_tag="JNX27_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY7882754.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKIQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="JNX27_001503"
     CDS             complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="JNX27_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY7882755.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26780..27895)
                     /gene="malK"
                     /locus_tag="JNX27_001504"
     CDS             complement(26780..27895)
                     /gene="malK"
                     /locus_tag="JNX27_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY7882756.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28260..29450
                     /gene="malE"
                     /locus_tag="JNX27_001505"
     CDS             28260..29450
                     /gene="malE"
                     /locus_tag="JNX27_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY7882757.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29604..31147
                     /gene="malF"
                     /locus_tag="JNX27_001506"
                     /pseudo
     CDS             29604..31147
                     /gene="malF"
                     /locus_tag="JNX27_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31162..32052
                     /gene="malG"
                     /locus_tag="JNX27_001507"
     CDS             31162..32052
                     /gene="malG"
                     /locus_tag="JNX27_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY7882758.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8229 a         8289 c         7801 g         8008 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtgaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaagttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aataatgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt ttacgtgcat
    14221 gccataaatt atgcgtgatt cgttgaataa ataaatctat ttttgcaata attatatccc
    14281 gatgacgcag cgctgcataa tcaatagatt taccatatat attatttaat ttacctttga
    14341 ttgaacctct attatccaat ttatctttgg ttgcatcttt attttcgaat tgatttgaat
    14401 tgagtgtttt attaaagtat ccagttgcac cgggtattat cttcaacatg attcatctac
    14461 cttttttgtc gtaccgtgtc ctccttataa ttctttttcc atctggtcac tatcaaaaat
    14521 cgctcataat ttctgaacta caggtatgcg tttaaaccca gttaaataaa tagcgagtta
    14581 acttgaagca atttatttaa aaatgaatta ttaattatta tcgtgttgat aatggtacga
    14641 caaggcaaca caacacctta cctacaaaac agaaaatcaa cattacggca acaataaggg
    14701 ttctcggtgt ttttcctgga acatggtaaa gtaaggacat tcttaacccc cactttgagg
    14761 tgcccgatgg aaaagaccac aacgcaggag ttattagcgc aggctgaaaa aatctgcgcg
    14821 cagcgtaatg tgcgcctgac cccacagcgc ctggaagtgt tgcgcctgat gagtctgcag
    14881 gatggcgcta tcagcgctta tgatctgctt gatttactgc gcgaagctga accgcaagcc
    14941 aagccgccaa cggtttatcg cgcgctggat tttctgcttg agcaaggttt tgtgcataag
    15001 gtggaatcca ccaacagtta tgtgctctgt catctgttcg accagcccac gcatacgtca
    15061 gccatgttta tttgcgatcg ctgcggcgcg gtgaaagaag agtgtgcaga aggcgtggaa
    15121 gacattatgc atacgctggc ggcaaaaatg gggtttgccc tgcggcataa tgtgattgaa
    15181 gcacatgggc tctgtgcggc atgcgtggaa gtggaagcgt gtcgtcatcc tgaacagtgc
    15241 cagcatgatc actctgtgca ggtgaaaaag aaaccgcgtt aagagggcgt acatccttgt
    15301 acacgtcggg caggagggat taattaccag cgatattcat tgcgggtttc ccaatccacg
    15361 acctcttttt ctgcctgatc tttctgataa ccataacgtt cctggatttt accgaccagt
    15421 tgatcacgtt taccttcaat gatcgtcata tcatcatcgg tcagtttgcc ccattgctct
    15481 ttcactttac ctttaaactg tttccagtta ccgccggctt catctttatt cataatcaag
    15541 acctcatcgt taggttgtga atgagagtac gttcactttt cttctgaacg tgagattaag
    15601 tgtagtcagc aatttggctt aggataattt attcagaatt tttaaccgtc acgttgcggc
    15661 aaaccaggta ccgttgcgcc agtgacgccg ccagatagcc gccagagaaa gcccgcgcaa
    15721 cgccagaaag acggttaatg ccagccacaa accatgatta cccagccacg gcagcgtaag
    15781 gagcgtcagc gcaaaacctg cggcggccac cgccatactg ttacgcattt cggcggcacg
    15841 cgttgcgcct ataaacatgc cgtccagcag ataacaccag acgccaacca acggcaaaat
    15901 cacctgccag ataagatagc ggtcagccag ctgctgaatc tgggttaacg acgtcagcaa
    15961 cgcaatgatg tgttcccctg ccagcaaata aaccaccgaa aacagtaacg ctacaatccc
    16021 cgactggcgg cacgctgccc gccatacatc cagcaactgg ctaccgtcgc gcgcaccgta
    16081 tgcctgaccg gagtgcgctt caaccgcgta ggcaaaacca tcaagcgcat aggcggtaaa
    16141 ggtgagtagc gtcatcagaa ccgcgttaac agcgataatg tcactcccca gtcgcgcacc
    16201 aagtacggtg atcgcgccga aacagagttg caacaacagc gaacgcagca tgatatcgcg
    16261 gttaagcgcc agcaagcgac ggaagtttcc tcgccaggca gttttcagca tttcgccgga
    16321 aattccgcgt agtttgagga ttttacgcac cattagcaga ccaatcagca atgttgcata
    16381 ttccgcaata accgtcgcca gcgccgcgcc ctgcacgttc atatgcagcc ccatcaccag
    16441 ccagacatcc agcacaatgt tgaggatatt gccgaccact aacaaaatta ctggcgcacg
    16501 ggcatattgc acgccgagta accaaccgag taataccaga ttcgccagcg acgccggagc
    16561 gcttaaccag cggatttcaa gaaagcgccg cgcctgttcc aggaccgctt cactaccgcc
    16621 aacaatatgc agcgccagat cgataatcgg cgtacgcaac agcgcaatta acgccccggc
    16681 ccccaacgcc aacagcaacg gttgcaccag cgcacgggct aatgcctgag gatttttggc
    16741 accataagcc tgcgcagtca gcccggtggt gctcatgcgt aaaaacagca acagcataaa
    16801 gagaaagctg gtcgccgttg caccaaccgc cacgccgccc aaataaaccg gactatcaag
    16861 atgaccaatt accgccgtat cgaccagtcc cagcaacgga acggtgatat tggagaaaat
    16921 catgggtaag gcgagatgcc agagtgcttt atcagatgaa gtgaggaatg ccatgcagac
    16981 aagcctgatg aagagagatg aaaaacaaac cgcgacgcca ggcggcatcg cggtctcaga
    17041 gatatgttac agccagtcgc cgttgcgaat aaccccaacc gccagccctt caatggtgaa
    17101 gctctgctga cgaaggtcaa cgacaattgg tttaaactcg ctattttctg gcaacagttc
    17161 gactttattg ccctgttttt tcaggcgctt aacggtaact tcgtcatcaa tacgtgcgac
    17221 aacgacctga ccgttacgta catcctgagt tttatgcact gccagcaagt cgccatccat
    17281 aatgccgata tctttcatcg acatcccgct gacgcgcagc aggaaatcag cattcggctt
    17341 gaacaaggaa ggatcgacct gataatgacc ttcaatatgc tgttgcgcca gaagcggttc
    17401 accggcagcc acacgaccta ccagcggcaa cccttcttcc tcttcctgca acagacgaat
    17461 cccgcgtgat gcgccggaaa caatttcaat aacgcctttg cgtgccagcg ccttcagatg
    17521 ttcttcagcc gcgtttgggg aacggaaccc caaacgctgc gcgatttccg cacgcgtcgg
    17581 cggcatacct gtctggctga tgtgatcacg gatgagatca aacacctctt gttgcctggc
    17641 cgttaacgct ttcattccgc cccctgggtg tatatacagt tatactgtga gtatatacag
    17701 caaaaggcga ttttggaacc ataaactgca caataaacca gagatttatc gaaatctgga
    17761 agggttatcc aaaatgcgac cataacagga tgcaccaggt aatcacggcg acgataatgg
    17821 caatcagcac cgcggcggat cccatatctt ttgcgcgtcc ggaaagctca tggtattcag
    17881 agccaattcg gtcaaccact gcttcgatgg cgctattgag gatttccaca atcatcacca
    17941 gcatcacgga gctgataagc agcacgcggg taattgcgtc cacatccagc cagcaggcga
    18001 tgaccaccgc caacaatacc gctacgcctt cctgacggaa tgccgcttcg ttgatccatg
    18061 cagcgcgtaa acctttccag gaatagccag cagctttgat aattcgggtg aatccagtgg
    18121 tattattgac cattgaaaga acctttttac attatgagcg tcaatatcag tgtaccgtta
    18181 aatccgatga ttcgggtttg cgtaacgccg cgcgaaacat gagcggatac cacagaattt
    18241 cccatgactt tctgctatcc ttgccgcgca tttgcattat taaccagagg ctttacatcg
    18301 tttatgtccg gctggccacg aatttactac aaattactga atttaccatt aagcatcctg
    18361 gtaaaaagca agtctattcc ggcagatcct gccccggaac tggggctgga tacctctcgt
    18421 ccaattatgt acgttttacc gtacaactcg aaagcagatt tgctgacgtt gcgcgcccag
    18481 tgtctggcac atgatttgcc tgacccgtta gagccgctgg aaatcgacgg cacgctactg
    18541 ccgcgctatg tgttcattca cggcgggccg cgtgtattca cctattacac gccgaaagaa
    18601 gagtctatta agcttttcca cgactatctc gatttgcacc gtagcaaccc aaatctggat
    18661 gtgcagatgg tgccagtatc ggtgatgttt ggtcgcgcgc cggggcgtga aaaaggcgaa
    18721 gtgaacccgc cgctgcgtat gcttaacggc gtacagaaat ttttcgctgt actgtggctc
    18781 ggtcgcgaca gttttgtgcg tttctcgccg tcagtgtcgc tgcgccgtat ggcggatgaa
    18841 cacggcacgg ataaaactat cgctcagaaa ctggcgcgcg tggcgcgtat gcactttgcc
    18901 cgtcaacgtc tggctgccgt aggcccacgt ctgcctgctc gtcaggatct gtttaataag
    18961 ctgctcgcct cccgcgccat tgccaaagcg gtagaagatg aagcgcgcag caaaaaaatc
    19021 tcccatgaaa aagcgcagca gaacgcgatt gcgctgatgg aagagattgc ggcgaatttc
    19081 tcttacgaga tgatccgcct gactgaccgc attctgggct tcacctggaa ccgattttat
    19141 cagggcatca acgtccataa cgccgagcgc gttcgccagc tggcccacga cggtcatgag
    19201 ctggtgtatg tgccttgcca ccgcagtcac atggactacc tgctactttc ttacgtgctg
    19261 taccaccagg ggctggtgcc gccgcatatc gccgccggga tcaacctgaa cttctggccg
    19321 gccgggccga ttttccgccg tctgggggcg ttctttattc gccgtacttt taaaggcaat
    19381 aaactttatt ccaccgtttt ccgtgagtat ctcggcgaac tgttcagccg tggttattcc
    19441 gtcgagtact tcgtggaagg cggtcgttcc cgcacggggc gtttgctgga tccgaaaacc
    19501 ggtacgctgt cgatgaccat tcaggcgatg ctgcgtggcg gcacgcgtcc gattacgctg
    19561 attccgatct atatcggtta tgagcacgtc atggaagtgg gtacttacgc caaagaactg
    19621 cgcggcgcga cgaaagagaa agagagcctg ccgcagatgc tgcgcggttt aagcaagctg
    19681 cgtaatctcg gtcagggtta cgtcaacttc ggtgaaccaa tgccgttgat gacctacctt
    19741 aaccagcacg taccagactg gcgtgaatct atcgatccca tcgaagcggt gcgtccggcc
    19801 tggttaacgc cgacggtcaa taatattgct gccgatctga tggtacgcat taacaacgca
    19861 ggcgcggcaa acgccatgaa cctgtgctgt actgcgctac tggcatcacg tcagcgctca
    19921 ctcacccgcg agcagttaac cgagcaactc aactgctacc tggatctgat gcgcaacgta
    19981 ccttactcca cggactctac cgttccttca gccagcgcca gcgagcttat cgatcacgcg
    20041 ctgcaaatga acaagtttga agtcgagaaa gacactatcg gcgacatcat cattctgcca
    20101 cgcgagcaag cggtgctgat gacctactat cgcaacaaca ttgcgcatat gttggtgctg
    20161 ccttcgctga tggcggcaat tgtcacccag catcgccaca tctcccgcga cgtattgatg
    20221 gagcacgtca atgtgcttta cccaatgctg aaagcggagc tgttcctgcg ctgggatcgc
    20281 gacgagttgc cggacgttat tgatgcgctg gcaaatgaga tgcaacgtca ggggctgatt
    20341 accctgcaag atgatgagtt gcatatcaac ccggcgcatt ctcgcaccct acagctgctg
    20401 gccgcaggcg cgcgcgaaac gctgcaacgt tatgccatca ccttctggtt gttgagtgcc
    20461 aacccgtcga tcaaccgcgg tacgctggag aaagagagcc gcaccgttgc gcaacgtctc
    20521 tccgtgctgc acggcatcaa cgcgccggag ttcttcgaca aggcggtgtt cagttctctg
    20581 gtgctgacgc tgcgtgatga agggtatatc agcgatagcg gcgatgccga accagcagaa
    20641 acgatgaagg tttatcagtt gctggcggag ttgattacat cagacgtgcg tttgacgatt
    20701 gagagtgcga cgcagggcga agggtaatca gagagaattg ccggatgcgg cgaaaacgcc
    20761 ttatccggcc taccatgacc tgcaaattca ataaattgcg attcaccggg taggcctgag
    20821 aagcgcagcg catcaggcaa cccagaagaa agccggatga tcatccggct tttttacatc
    20881 atcagaaatg ccagtaactc attgccagtc ctaaaaacag taccagacca acatagttat
    20941 tattcataaa tgctttaaag caggcttcac gctcgcggtt ggcaatcagt ttttgttgat
    21001 aaacaaacag cgcgctagcc accacaattg accagtaata tccccagcct aagccattta
    21061 actcaccgat gatcgccatc agtgccagta cgccaatctg caaaatacca ataatcaatt
    21121 tatcgtattg gccgaacagg attgccgtgg atttaatgcc aatcttcaca tcatcatcgc
    21181 ggtcaaccat cgcatactgc gtgtcgtaag ccaccgccca gagaatattg gcgaggaaca
    21241 ttaaccagca actcaatggc accgactcac tcacagcggc aaaagccatt ggaatcgacc
    21301 agccaaacgc cgcgcccagc accacttgcg ggagatgggt ataccgcttc ataaacgggt
    21361 acacccacgc caatgccaga gcggcaatcg acaacagaat ggtcatcgta ttcagcgtca
    21421 gaaccagtaa aaacgaaatc agtaccagca ctacaaacag cgcgcgcgcc tctttctctg
    21481 ttaccgcgcc gctgggaagt ggtcggttcg ccgtgcgctt aacatgacca tcaaacttgc
    21541 gatcagcata atcattcacc acgcaaccag cagcgcgcat cagccagaca cccgcgacaa
    21601 acaccgccag gatccagagc tgaggaacgc ccggtgtcgc cacccacaac gcccataatg
    21661 ttggccagag cagcagtaac gcgccaattg gcttatccgt acgcattaag cgatgaaacg
    21721 ccagcagctt attctgcgtc agactccact ccatattttt ttcctcttag tacaacggtg
    21781 acgccggtaa aaacagttct gttagcaaca gcggtttacc gcttaatcgc aggcgggaac
    21841 gtcgccccca cagtccggca tcacggccta tctcgataaa atcccgggtt agtgtcgatg
    21901 atgtgaacag atagcgtcct aacggcgttt tacccaattt ttgtaacgct agttccggcc
    21961 cgcttaatgt tgatacagga acgacggtac gaccggcaag ccacggttca ccatcggcac
    22021 ataacaaaat ttcacgtaac cagtaacgag actctttcgg cagtagcggc agttcttcgg
    22081 ggatttcatt ctgctcgaca aacccctcgc ggatcatcgt cacactcacc gtttttccct
    22141 gctgttcaaa acgttttgtc atggaatcct ccagcaacag ccagtcgaac agttgcggat
    22201 ccagggcagg gatctcttta aaatagcgca gcgcacgcag ttgcgttaac gcggggtgtg
    22261 acatgccgaa ctctccgtta cataacgtaa aggcattgta tcgcagaaaa gtgtttacgg
    22321 gagatgtaaa gcaaattaag tgatctaatg cgcaacaaaa acgcaacaat cgcatgaagt
    22381 ggtaaaacgc tgttgaacct tacgctttgc cgatgcccgt gctggcgagg gcaaccatag
    22441 gggcatcacc gcccatgatg ctaggaacgg tgatgttttc ctttacttaa atacctttat
    22501 tctttatacg agtagctcgc caactcgcag taaaaattgg gaaaagcgca tatttaccat
    22561 gagatttgga gctgaaaagt gtttcctgag taagattaat aggcaacatc ttatgtctgg
    22621 atttatagat acacgtggaa ttccaattgg tatcttttgt aggattcaac atatcatgta
    22681 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
    22741 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
    22801 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
    22861 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
    22921 tatttttatc aaccagccct ttattcatat taaatctgtt aataaaatgg atcatctgtt
    22981 gggtatcgaa attcacctta ctgaatcgat gttgacgcat aaaattatcg aacttgtcat
    23041 cttccccaga atgtatataa ctacgtataa ttggatcttt tatataagga caatggctaa
    23101 atgttttctt catattctca ataaagtttt tattaacaat attgagaatt tttaattgca
    23161 tcaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
    23221 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
    23281 aatcaatagt taattccaat tgttctgtaa ttcaatctta aaaattccct gtcgacattc
    23341 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
    23401 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
    23461 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
    23521 tatcaagaat attttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
    23581 tgtgtaaacg tcgacattta aatatgacat tatgaactgt tgccttagta aaattgcaat
    23641 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
    23701 tagaaataac gtcaccttta aaatgacaat ttgttaaatc aaaatcagac aggtcgatat
    23761 acccctgcgc atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
    23821 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
    23881 gatttttttc gttccgttgt tttatattta aaaatatatc actgatataa atgaagttat
    23941 ctctgtttaa acctgtcacc ccattgaaat tacgtagtaa atcaagatct actatattat
    24001 ttccttgcac cacatcatgt ttcatcaaag agttattgcc gccagaattt gtgatatgac
    24061 ctatcataaa acctctgtaa tacagaaata aaataacctg gaaaagtagc ctgagcgtcc
    24121 agacactacc ggcgaaagga aaagaatctc tgataatgca ttgaaataat ggacattctt
    24181 agtgaagaag aagtgaagta ctataaaaaa tcgctcatat cccaggaagg aagcataaaa
    24241 aaaggtgcgc caggagacgc accagttgtt gcaaaatcag cactgtgggg cgtaattacc
    24301 ccttgccttt tacactgctg ataaaggtgg aacgggcaga tgtcgatccc aggcgttcag
    24361 cttcatcaag cagttttaac gccttatcaa catcaccttt cgcgacagcg tttttgatcg
    24421 cggtattaaa ataactttcc gtgtcgttga gcatcggctc gcttttcttc accggcaccg
    24481 gagcaggtgc agccacagct ggtgccgccg tgttgcctac cgtaaccgga gctggagcgg
    24541 aagaaccaaa tagtggtcct accaacacgc tggagctgga gttcgttttg actttcagtt
    24601 tcagtaagcc atcgctggta tgacgagcaa ccggatcggg aatatccggg atcgagttac
    24661 cgacgccttt ggcataggct ttagccgggt cgagcagttg ggtcgtctgc tggagatctt
    24721 tttccatggt aaagaccaga acataaagtt tttgctgccc caacgccggt gtcaggcgca
    24781 taacgccttc cagccgatct gcactcatca cgcctagttc ctggtaggtg aaataactgc
    24841 tggggaagaa ggctgatggg gtcatgttct gatcaataat cagcacgttc ggcgcaaaaa
    24901 cgctggtttg tttgttcact tcgctggtca gcgtcagggt cagttcgcca atgtttgccg
    24961 ggacgctgta cgcagcaacc ggaccactga tgccgggaac gttcagttgt tggccgccgg
    25021 tcgccagttg ggtggtctgg attttagatt gatcgaccgg tgtccaggtg agttgttgca
    25081 gcgcggcaga tggaatggct ggcgcgtcgc tggtgttttg cggtacgtag ttaacatcgg
    25141 caaggctaat tccaggcgcg cttgccagta accctgctga taaacagagg gcgatgagac
    25201 ttttattcat tttcattgtt ttcacctcaa aatctggagc tcagcggtag ccaggcaata
    25261 gcgcgctaaa cccgataatc agaggggctt tcgccccttt cagataatga caacctgttt
    25321 ttatgccgga tgcggcgtaa acgccttatc cggcccaggt tttgctatta ccaccagatt
    25381 tccatctggg caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct gccgccgttg
    25441 aaatcagcag gaacggcttt gccgtagttc gggttaacct tgctatcgcc gttgtagtcg
    25501 taaccccatt tctcatccca cttggcgtag gttgcgaaga cacgaatagc cgggcgtgac
    25561 cagatgctgt cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg
    25621 tcgccggtgc gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt
    25681 ggcgtccact tgtacatcgg gcgaatacca acggtccacc acttggtgcc gttgtcgtta
    25741 tcccagttga tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag
    25801 atcgcaccgt ggtcgaggat acgcagcatg tgaccgttgt tgttgatatt gtaggcaaat
    25861 ttttcgttat caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc
    25921 gagtcagtag cgtactgaac aacaaacttg ttaaagccct tcaggacact ctgagtatgt
    25981 tcagcagtga ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc
    26041 aggttggcac gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc
    26101 tgcgctaaac gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg
    26161 ttgctggcaa aagaggaaga accaccagct tcagaggagc gggttgctgc cagagagagt
    26221 ttaccgaagc caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag
    26281 tagaagtcga tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg
    26341 gaacctggca gccattcgat caggtttttt ccctgcacgt ttgcttcacg gaaggccgga
    26401 tcggtagctt cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag
    26461 aagctcttat cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca
    26521 cattcgttgc caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttca
    26581 ccgccgctac ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt
    26641 gcctgagcag acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta
    26701 atcatcattc tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt
    26761 tgtgtgtttt gtggggtgct taaacacccg gctccttatg cagtcgacga catgcagtgc
    26821 catcctcacg gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt
    26881 cttctaccaa caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct
    26941 ggatatagat ttgggtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga
    27001 tgacgtcagc gatatcactc ggcagtagat gttccgggcg aatacccagc gacatattgg
    27061 ctccaacctg gacatcacgg ctttcaacag gcagccagac ttgttgacga tttggcatcg
    27121 gcagctccac ctgcacttga tcgattgcgg tggcggtcac ttttaccggc aggaagttca
    27181 tctttggcga accgataaat ccggcgacaa aacggtctgc cggatagtgg tacagttcca
    27241 gcggtttccc aacctgtgcc acgcgaccgg cgtccagcac cacgattttg tcggccagcg
    27301 tcatcgcttc gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca
    27361 gacgggagat ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt
    27421 catcgagcaa aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acgcgctgac
    27481 gctgaccacc ggagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt
    27541 ccgccacctg gttgacgcgt tggttaatca cctctttttt tgcgccagcc agtttcaggc
    27601 caaatgacat gttttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca
    27661 taccaacgcc gcgttctgct ggcggagtgt cattcatccg tttctcaccg atgaacaggt
    27721 cgccgctggt gatcgtctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc
    27781 cagacggtcc gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg
    27841 ataccacgac ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct
    27901 tctccctttg taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca
    27961 tcaggagatg gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga
    28021 tgagaacgcg gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt
    28081 gcaaaaatcg tggcgatttt atgtgcgcat ccccacatta ccgccaattc tgtaacagag
    28141 atcacacaaa gcaacggtgg ggcgtagggg caaggaggat ggaaagaggt tgccgtataa
    28201 agaaactaga gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta
    28261 tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt
    28321 ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
    28381 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
    28441 tcaccgttga gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg
    28501 atggccctga cattatcttc tgggcgcacg accgctttgg tggctacgct caatctggcc
    28561 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacatggg
    28621 atgccgtacg ttacaacggc aagctgattg cttacccaat cgctgttgaa gcgttatcgc
    28681 tgatttataa caaagacctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
    28741 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
    28801 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
    28861 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
    28921 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
    28981 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
    29041 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
    29101 ctaaaccgtt cgttggcgtg ctgagcaccg gtattaacgc cgccagtccg aacaaagagc
    29161 tggcgaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
    29221 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
    29281 cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
    29341 agatgtccgc tttctggtat gccgtacgta ctgcggtgat caacgccgcc agcggtcgtc
    29401 agactgtcga tgaagccctg aaagacgcgc agactcgtat caccaagtaa tgctgtgaaa
    29461 tgccggatgc ggcgtgaacg ccttgtccgg cctacaaaac tgaaacgcat gtaggcctga
    29521 taagacgcat cagtgtcgca tcaggcagtt gttgtcggat aaggcgtgaa agccttatcc
    29581 gtcctggaat gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg
    29641 acgcgctgaa atggtcagtg ctaggtctgc tcggcctgct ggtgggttac cttgttgttt
    29701 taatgtacgc acaaggggaa tacctgttcg ccattaccac gctgatattg agttcagcgg
    29761 ggctgtatat tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg
    29821 ctggaatggg attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca
    29881 actacagcag cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ttagatcgct
    29941 cctggcaagc aggcaaaacc tataactttg gtctttaccc ggcgggcgat gagtggcaac
    30001 tggcgctcag cgacggcgaa accggcaaaa attacctctc cgacgcgttt aaatttggcg
    30061 gcgagcaaaa actgcaactg aaagaaacga ccgcccagcc cgaaggcgaa cgcgcgaatc
    30121 tgcgcgtgat tacccagaat cgtcaggcgc tgagtgacat taccaccatt ctgccggatg
    30181 gcaacaaagt gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac
    30241 tcgacggtga cggcacgttg acgaataatc agagtggcgt gaaatatcgt ccgaataacc
    30301 aaattggctt ttaccagtcc attaccgccg acggcaactg gggggatgaa aagctaagcc
    30361 ccggttacac cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca
    30421 ttcagaaacc gttcctcgcc attttcgtct ggaccgtggt gttctcgctg atcactgtct
    30481 ttttaacggt ggcggtcggc atggttctgg catgtctggt gcagtgggaa gcgttgcgcg
    30541 gcaaagcggt ctatcgcgtc ctgctgattc tgccctacgc ggtgccatcg ttcatttcaa
    30601 tcttgatttt caaaggattg tttaaccaga gcttcggtga aatcaacatg atgttgagcg
    30661 cgctgtttgg cgtgaagccc gcctggttca gcgatccgac caccgctcgc acgatgctga
    30721 ttatcgtcaa tacctagctg ggttatccgt acatgatgat cctctgcatg ggcttgctga
    30781 aagcgattcc ggacgatttg tatgagcctc agcaatggat ggcgcaggtc cgttccagaa
    30841 cttctttaag attacgctgc cgctgctgat taaaccgctg acgccgctga tgatcgccag
    30901 cttcgccttt aactttaaca acttcgtgct gattcagctg ttaaccaacg gcggcccgga
    30961 tcgtcttggc acgaccacgc cagccggtta taccgacctg cttgttaact acacctaccg
    31021 tatcgctttt gaaggcggcg ggggtcagga cttcggtctg gcggcagcaa ttgccacgct
    31081 gatcttcctg ctggtgggtg cgctggcgat agtgaacctg aaagccacgc gaatgaagtt
    31141 tgattaaggg agataacaaa aatggcaatg gtccaaccga aatcgcaaaa agcacgtttg
    31201 tttattactc acctgctact gctacttttt atcgcggcga ttatgttccc gctgctgatg
    31261 gtcgtcgcta tctcgctgcg tcagggaaac ttcgcgaccg gtagcctgat cccggagcaa
    31321 atctcctggg atcactggaa actggcgtta ggttttagcg ttgaacaggc tgatggtcgc
    31381 attacgccac cgccattccc ggtactgctg tggctgtgga actcggtaaa ggtcgctggg
    31441 atttccgcga ttggcattgt ggcgctctcc accacctgcg cctacgcttt cgcccgtatg
    31501 cgctttccag gcaaagcgac gctgctgaaa ggaatgctga ttttccagat gttcccggca
    31561 gtactttcac tggtcgcgtt gtatgcgttg tttgatcgtc tgggtgagta cattccattc
    31621 attggcctga atactcacgg cggcgtaatt ttcgcgtatc tgggtgggat tgcgctgcat
    31681 gtctggacca tcaaaggcta tttcgaaacc atcgacagtt cgctggaaga agcggctgcg
    31741 ctggatggtg cgacaccctg gcaggccttc cgccttgtcc tgttgccgct gtcagtaccg
    31801 attctggcgg tggtattcat cctgtcgttt atcgctgcca ttactgaagt tccggtcgcg
    31861 tcgctgttac tgcgtgacgt aaacagctac accctggccg tggggatgca gcaatacctc
    31921 aacccgcaaa actacctgtg gggtgacttt gccgccgctg ccgtgatgtc tgcattaccg
    31981 atcaccatcg tcttcttgct ggctcaacgc tggctggtca acggcctgac ggcaggtggt
    32041 gtgaaaggtt aaagatgttg ttctgccaat gttatgccgc tgcaccctca acttacgtta
    32101 tcccaacttg tgactattat tcggcgctcc acggagcgcc tttttttctt tcgtctgcaa
    32161 tctgaatcgt tcgccggtta atattcccat catagaactt attattttta cgttatttgt
    32221 tttcccactt acgataattc tctttcgtgc tctgagtcac ggcaatagta ttgttttcat
    32281 caatttttga taattatcac aattaagatc acagaaaaga cattacg
//
LOCUS       DADLXX010000032        31488 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLXX010000032 DADLXX010000000
VERSION     DADLXX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.032"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(2..94)
                     /locus_tag="JNX27_001508"
     tRNA            complement(2..94)
                     /locus_tag="JNX27_001508"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNX27_001509"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNX27_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7882759.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNX27_001510"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNX27_001510"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7882760.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNX27_001511"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNX27_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7882761.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNX27_001512"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNX27_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7882762.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNX27_001513"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNX27_001513"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7882763.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNX27_001514"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNX27_001514"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7882764.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8018
                     /gene="srlA"
                     /locus_tag="JNX27_001515"
                     /gene_synonym="gutA"
     CDS             7455..8018
                     /gene="srlA"
                     /locus_tag="JNX27_001515"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY7882765.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8015..8974
                     /gene="srlE"
                     /locus_tag="JNX27_001516"
                     /gene_synonym="gutE"
     CDS             8015..8974
                     /gene="srlE"
                     /locus_tag="JNX27_001516"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7882766.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8985..9356
                     /gene="srlB"
                     /locus_tag="JNX27_001517"
                     /gene_synonym="gutB"
     CDS             8985..9356
                     /gene="srlB"
                     /locus_tag="JNX27_001517"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7882767.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9360..10139
                     /gene="srlD"
                     /locus_tag="JNX27_001518"
     CDS             9360..10139
                     /gene="srlD"
                     /locus_tag="JNX27_001518"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7882768.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10244..10603
                     /gene="gutM"
                     /locus_tag="JNX27_001519"
     CDS             10244..10603
                     /gene="gutM"
                     /locus_tag="JNX27_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY7882769.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDILFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10670..11443
                     /gene="srlR"
                     /locus_tag="JNX27_001520"
                     /gene_synonym="gutR"
     CDS             10670..11443
                     /gene="srlR"
                     /locus_tag="JNX27_001520"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7882770.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11436..12401
                     /gene="gutQ"
                     /locus_tag="JNX27_001521"
     CDS             11436..12401
                     /gene="gutQ"
                     /locus_tag="JNX27_001521"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7882771.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNX27_001522"
     CDS             complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNX27_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7882772.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRVVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14099..15538
                     /gene="norV"
                     /locus_tag="JNX27_001523"
     CDS             14099..15538
                     /gene="norV"
                     /locus_tag="JNX27_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7882773.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15535..16668
                     /gene="norW"
                     /locus_tag="JNX27_001524"
     CDS             15535..16668
                     /gene="norW"
                     /locus_tag="JNX27_001524"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY7882774.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNX27_001525"
     CDS             complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNX27_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7882775.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNX27_001526"
     CDS             complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNX27_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7882776.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19877..20890)
                     /locus_tag="JNX27_001527"
     CDS             complement(19877..20890)
                     /locus_tag="JNX27_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7882777.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            21147..22604
                     /gene="ascF"
                     /locus_tag="JNX27_001528"
     CDS             21147..22604
                     /gene="ascF"
                     /locus_tag="JNX27_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7882778.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            22613..24037
                     /locus_tag="JNX27_001529"
     CDS             22613..24037
                     /locus_tag="JNX27_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7882779.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFHEKGVTIDKALGDDEILKNTVDFVSFSYYTSRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24152..24571)
                     /locus_tag="JNX27_001530"
     CDS             complement(24152..24571)
                     /locus_tag="JNX27_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7882780.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24568..24879)
                     /locus_tag="JNX27_001531"
     CDS             complement(24568..24879)
                     /locus_tag="JNX27_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY7882781.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25052..25795)
                     /locus_tag="JNX27_001532"
     CDS             complement(25052..25795)
                     /locus_tag="JNX27_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882782.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(25847..26317)
                     /gene="hycI"
                     /locus_tag="JNX27_001533"
     CDS             complement(25847..26317)
                     /gene="hycI"
                     /locus_tag="JNX27_001533"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY7882783.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26310..26720)
                     /locus_tag="JNX27_001534"
     CDS             complement(26310..26720)
                     /locus_tag="JNX27_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY7882784.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26717..27484)
                     /gene="hycG"
                     /locus_tag="JNX27_001535"
     CDS             complement(26717..27484)
                     /gene="hycG"
                     /locus_tag="JNX27_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY7882785.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27484..28026)
                     /gene="hycF"
                     /locus_tag="JNX27_001536"
     CDS             complement(27484..28026)
                     /gene="hycF"
                     /locus_tag="JNX27_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY7882786.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28036..29721)
                     /locus_tag="JNX27_001537"
     CDS             complement(28036..29721)
                     /locus_tag="JNX27_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7882787.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIACDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29739..30635)
                     /gene="hycD"
                     /locus_tag="JNX27_001538"
     CDS             complement(29739..30635)
                     /gene="hycD"
                     /locus_tag="JNX27_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7882788.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(30638..>31488)
                     /locus_tag="JNX27_001539"
     CDS             complement(30638..>31488)
                     /locus_tag="JNX27_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882789.1"
                     /translation="YHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISI
                     AMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAV
                     MCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAA
                     VPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKS
                     MVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVI
                     IVSRGA"
BASE COUNT         7406 a         8711 c         8085 g         7286 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaacga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tacatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaagttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttgcgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggaa tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgctggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctaggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacgctga
     7861 acctgccact tggcccactg gcggtgagtt atctgctggt tggtctggtc accaatttct
     7921 tccgcggctg ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac
     7981 ttgaacaaaa agttcacctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg
     8041 aacgggtggc tggggcggcc cgcttgagct ggaagctacg ccgggcaaaa aaatcgtcta
     8101 tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg gcacaactta ctggctggca
     8161 ggctattgac ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat
     8221 cgactgtggc ggcacattac gctgcggcat ctatccgaaa cgacgtattc ccaccattaa
     8281 tatccactcg acgggcaagt ccggtccgct ggcgcagtac attgtggaag atatttatgt
     8341 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
     8401 cgtgggccgt gactatgaca ccagtaagaa aatcaccgaa caaagcgatg gtttactggc
     8461 gaaggtggga atgggcatgg ggtccgccgt tgccgtgttg tttcagtctg gtcgtgacac
     8521 catcgacact gtattaaaaa ccattctgcc gtttatggca ttcgtctcgg cgctcattgg
     8581 catcattatg gcttctggcc ttggtgactg gattgcccac ggtcttgctc cgctggcgag
     8641 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccactgc tttcaccttt
     8701 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
     8761 tctcggcaat attccgccgc atctggcttt accggcactg tttgccatca acgcgcaggc
     8821 ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
     8881 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcgccga ctgtactgat
     8941 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc
     9001 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
     9061 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
     9121 gcgagttgaa aggtgcactc catcccggtt tgcaattttc actcgggcag catcgctatc
     9181 cggtgaccgc tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc
     9241 tgcgcttcga tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg
     9301 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
     9361 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
     9421 gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
     9481 atgtggcaca agaaattaac gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg
     9541 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttggtc
     9601 gcgtggattt gctggtctat agcgccggaa tagcaaaagc agcctttatc agcgacttcc
     9661 agctcggcga ttttgaccgt tcgctacaag tgaatctggt gggttatttc ctgtgtgccc
     9721 gtgaattttc gcgtttgatg atccgcgacg ggattcaggg tcgcattatt cagatcaact
     9781 cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg aaatttggtg
     9841 gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
     9901 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcactgttg ccacaatacg
     9961 cgaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    10021 tcaaacgcgg ctgcgattat caagatgtgc tgaatatgct gctgttctac gccagtccta
    10081 aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    10141 caacagcgga gatccgttaa ggatctccgt gagactatag aatgcctgat gcgctacgct
    10201 catcaggcat acaggacttc cgccactaca ttaaggaaaa gttatggtat ccgcactcat
    10261 caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggctggc aaatttctcg
    10321 ttttaaccgt gccttcgaca cactatgcca gcaagggagg gttggcgtgg gccgttccag
    10381 cgggcacttt aaaccgcggg tcgtggtcgc catcgcgctg gacgatcagc agtgcatcgt
    10441 cgacatcttg tttatgaaag gactgactgt cttcgcccga ccgcaaaaaa ttcccgcaat
    10501 taccggcatg catgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc
    10561 acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta
    10621 cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg
    10681 tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga
    10741 attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga
    10801 acatgccgga accgtcattc gtacttatgg cggagtggtg ttgaataaag aggaatccga
    10861 tccgcctatc gatcataaaa cgctcatcaa cacccacaag aaagagctga ttgcagaagc
    10921 tgccgttagt tttatccatg atggcgattc gatcattctt gatgctggca gtaccgtttt
    10981 gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca acagcctgca
    11041 tatcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac
    11101 atttcgcaaa aaatcggcct catttcacgg gcaactggca gagaatgcct tcgagcattt
    11161 caccttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac
    11221 gttcaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt
    11281 gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca gtcttgaaag
    11341 cgtcgataag ctaattaccg acgcaggtat cgatccggcg tttcgtcagg cgctggaaga
    11401 gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg
    11461 gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa cgtctgggcg
    11521 atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg
    11581 gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc agtaccggca
    11641 caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg atgattgaaa
    11701 gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg gatctgatta
    11761 ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac
    11821 cgctgggcct ggcggcgaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga
    11881 tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac gcgctggcga
    11941 tggcggtcat gcaggcgcgc ggttttaatg aagaagattt tgcccgctcc cacccagctg
    12001 gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgtcgt gatgacgcta
    12061 tcccacaggt agcgttaacc gccagcgtga tggatgcgat gctggaactc agccgcaccg
    12121 gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg
    12181 gcgatttacg tcgctggctg gtaggcggcg gcgcactcac cacgccagtt aatgaagcga
    12241 tgacgacagg cggcaccaca ttacaggcac aaagtcgcgc catcgacgcc aaagaagttc
    12301 tgatgaaacg caaaatcact gccgcaccag tggtagatga aaacggcaaa ctcaccggcg
    12361 caattaacct gcaggatttc tatcaggccg ggattattta atccttcaat cccagacgtt
    12421 tcgccagccg atgcaggttg gcgacgtcag tttccagcat tcgcgcacag gctgcccagt
    12481 tgtgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt
    12541 cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc aacgtcacct
    12601 caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg
    12661 ccagcactac cacccgatga atagcatgtt ccagttcgcg cacgtttccc ggaaaactgt
    12721 agtgttgcag taaatttcgc gccccggcac ttaataccac gcgggagagc ccctgccgca
    12781 aacgacactg ctcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca
    12841 gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caaatcggcg cggaatcgcc
    12901 ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc
    12961 gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc ctcagcagct
    13021 tggcctgcaa tgccaacgac aactcgccga tctcatcgag aaacagagta ccgttatccg
    13081 ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct ttcacatgcc
    13141 cgaacaactc actttccgcc acactttccg gcagtgcggc acagttgaga tagaccagcg
    13201 gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc
    13261 ccgtctcacc gctgatcaga acgttgagat cggacgccgc cacaatctca atctcttttt
    13321 tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg
    13381 gcgtggcatc gcctggcagc atattctggc tttccagttg ttcaatcagc aacgcattgc
    13441 ttaacgctcc cgccgccagc gcggcaatca gccgtaactc ttcgtcgctg aaaacatcga
    13501 actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgt ccgacaaaca
    13561 atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc aaaccgtcat
    13621 agggatcggg caattcgctg tctgcgggaa agcgcaccac atccccggcg cgggcaatcg
    13681 cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacatcc tttgccagac
    13741 cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc
    13801 actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc
    13861 cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct
    13921 cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag tcattttgac
    13981 tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa gaaactggca
    14041 cgcaatctgc aattagcaag acatcttttt agaacacgct gaataaattg aggttgctat
    14101 gtctattgtg gtgaaaaata acattcattg ggtgggtcaa cgtgactggg aagtgcgtga
    14161 ttttcacggc acggaatata aaacgctgcg cggcagcagc tacaacagct acctcatccg
    14221 cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca
    14281 gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta accatgcaga
    14341 agaggaccac gccggggcgc tgaccgaact gatggcgcaa attcccgata cgccgatcta
    14401 ctgtactgcc aacgctatcg actcgataaa tggtcatcac catcatccgg agtggaattt
    14461 taatgtggtg aaaactggcg acacgctgga tatcggcaac ggcaaacagc tcatttttgt
    14521 cgaaacacca atgctgcact ggccggacag catgatgact tacctgacag gcgacgcggt
    14581 gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt tcaacgatga
    14641 agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata tcctgacgcc
    14701 gttcagccgc ctggtaacgc cgaaaattac cgagatcctg ggctttaact taccagtcga
    14761 tatgatagcg acctcccacg gcgtggtctg gcgcgataac ccgacgcaaa ttgtcgagct
    14821 gtacctgaag tgggccgctg attatcagga agacagaatc accattttct acgacaccat
    14881 gtcgaataac acccgcatga tggctgacgc tatcgcccag ggaattgcgg aaaccgaccc
    14941 acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa
    15001 tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg tgatgatgcc
    15061 gaaaatcgct gggctggtgg aggagatgac cggattacgc ttccgtaaca aacgcgccag
    15121 tgctttcgga tctcacggct ggagcggcgg tgcggtggat cgcctttcca cgcgcctgca
    15181 ggatgcgggt ttcgaaatgt cgctcagcct gaaagccaaa tggcgaccag accaggacgc
    15241 tctggagtta tgccgtgaac acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct
    15301 gccgcagagc acggtgaata cggtagttaa agaagaaacc tctgccacga cgacggctga
    15361 cctcggccca cgaatgcagt gcagcgtctg ccagtggatt tacgatccgg caaaaggcga
    15421 gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata acttcctctg
    15481 cccggaatgc tccctcggca aagacgtctt tgacgaactg gcatcggagg caaaatgagc
    15541 aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc
    15601 aaacaggacg ccactattcc attaaccctg attgccgccg acagcataga tgagtacaac
    15661 aaacccgacc tcagccatgt tatcagtcag gggcaacgtg ccgacgacct tacccgccag
    15721 acggcgggtg aatttgccgg gcagtttaat ctgcgcctgt ttccgcacac ctgggtgacg
    15781 gatatcgatg ccgaagccca tgtggtgaaa agccagaata atcagtggca atacgacaag
    15841 ttagtgctgg caaccggtgc cagcgccttt gtcccgcctg tgcctgggcg tgagttaatg
    15901 ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga
    15961 cgcgtgttga ttgtcggcgg tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt
    16021 gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc attaatgcca
    16081 ccagaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca tctgctgttg
    16141 aaatctcagt tgcaggggct ggaaaaaacg gattctggca ttctggcaac gctggaccac
    16201 cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc
    16261 ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa
    16321 accagtaatg ccgatattta cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg
    16381 ccgttcctgc agccgattca acttagcggg atggtgctgg caaaaaatct tctcggcaat
    16441 aacacgccgc tgaaactacc ggcgatgctg gtgaaaatca aaacgccgga attaccgctg
    16501 catctggcag gcgaaaccca gcgtcaggat ttacgctggc agattaatac cgaacgccag
    16561 ggaatggtgg cgcgcggcgt tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag
    16621 gatcggatga aagaggcatt tgtattgttg aaaacgttgc cggtgtaggt gggctactgt
    16681 gcctaaaatg tcggatgcga cgctggcgcg tcttatccga cctacgggga cgcatgtgta
    16741 ggccggataa ggcatttacg ccgcatccgg caagggtgtc caaatgcaac acgctttatc
    16801 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    16861 aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    16921 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    16981 tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat
    17041 catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac tgctgccaga
    17101 aagtggcgag atccagttga ttgtccaccc gcggcatcgt caccggatgc gtcactccgt
    17161 cgcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17221 ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac gatgatgcca
    17281 gcggcgcgtt aattccacgc tcaatggccc gcgccagtac gctccagttt tgctgttgca
    17341 cacttgccgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca
    17401 ggtttcgcca cggctgcttc gccgccaaat cgccacccgg aagcgccact gcaggcaagc
    17461 cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc cacaaagcgc
    17521 cattctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg
    17581 gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt accgtttgcg
    17641 tcggcagatt catttcgctc gcccactggc tggagacata gcccggatgc acatcatgca
    17701 caatgtattg cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    17761 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac
    17821 cgcgcaccag gcagaaggta tttttcagat ccgcgccaag acacagcaca ggcggaacat
    17881 ttttaaagcc cggaggcaaa accagcgcat ccggcacata cccccgcgaa cggcgcagca
    17941 tttcgccgct ttcgcgcacc actgaatcat ccatccgctg cacgatgtcg cggttatgta
    18001 gcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg
    18061 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18121 gcaaatgctg gagcgggttc gcaggcaaca tcaccccgac ttcgttaagg ccaggggcga
    18181 tatcatcaca aagctcaggt acgtattttt tatccaccag cacaatcggc gcagcgggcg
    18241 tggtaagcaa ctggcgcgca gcgtctggta aactttctgc cactggcaac atgactgcca
    18301 gcggtttcgc cgggcgatgt ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg
    18361 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18421 actgtgcgat agctgcctgt aatgccgcct cttgttctgc atgttcacca tgacttaccc
    18481 attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18541 gcgggtcgcg gtactctttg tcacaggccg gacatagcgg aaacgccgcc atcacggtaa
    18601 acgggcggtc gtaaggcatc gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt
    18661 tgataaacgg ataacgataa cgccgctcgc ctggggtatt catttcggca aggcaagcag
    18721 ggcaagtggc ggcatccggg acaatttgcg tattcatggt gccgccggtg ctctggcgga
    18781 tggtgaactc ggtgggcagt tgtgaccaga taaacggctc acgctcgacg ctatcaatac
    18841 gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa cgtttccgga tcttccagca
    18901 gccggacttc tacgccatcg ccgtcattac atacatcgcc gtgaagattt aattgctgtg
    18961 ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19021 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19081 ccgccctgcc ggacacgaca gcgtcgtatc cggcagtctc aggtcggcga taccgcccgc
    19141 tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt
    19201 ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc agttgttcaa gtttattacg
    19261 atcgacacaa atcagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg
    19321 atggttgcac aggtcgcatt tattggcttc ggctttgtca gcccgtacat tcagacccgc
    19381 gccgctgttg cgaatcacag gacgtaccac cacttccatc gcgccatacg ggcaagccac
    19441 cacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg
    19501 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac
    19561 tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg
    19621 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19681 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19741 caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat ttgctgttat
    19801 ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc atcgacacta gtgacgatga
    19861 catgtgatga caatgtttat cgcgaaaggg caatgagtga gtcgcggcgg atcagttttc
    19921 cgctaaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa
    19981 taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat
    20041 agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc
    20101 cgcgctcgtg caacgctttg atcgcaccta tcgccatatc gtcgttactg gcaactaacg
    20161 cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg ctggcgggcg
    20221 tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc gacagcgcgt
    20281 ctttataccc ggcaagacgt tcaatgctgg tgggggaatc catcgagccg gtaaggaaag
    20341 caatctcctg atgcccggcg tttatcaact ctgccacggc gttaaagctg gtctgtttat
    20401 gatcgcacca gacgctatgg ctgctattat tgcgcaggcg gcgattaagc accattatcg
    20461 gctgactgtg cgcgtcaatg atgtcatcaa tctcatccac gcttaaaaaa cgcgggtaaa
    20521 tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg
    20581 cgctgtgttt accatctgcc aatagcaact gtcgcccttt ctcttccgcc attcgcgcgg
    20641 catgaaacag taattcacta aaataaatgc cgtgataaag cgtgttggtc actaccagcc
    20701 ccagcgtctg agtactcttc gccgacagat tgcgggccag caaatttggg cggtaactgc
    20761 tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg
    20821 aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg
    20881 tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat
    20941 tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat
    21001 gaacaattaa ctctcttgct tatttagtga caactattca tgattttgtg aaaccggttt
    21061 cttaattccg tttcagcatc ggtatttttc cgtcacgtcg actgataaca actacatcta
    21121 ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg
    21181 tgatagcggc actgggcggc gttgataaca tctcggcagt cacgcactgt atgacgcgtt
    21241 tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg
    21301 gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt aattatcggc aatacggttt
    21361 cgcaggcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc gcacagcccg
    21421 tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg gatcctcgat gcgctgatcg
    21481 gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga
    21541 tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg
    21601 tgattggtga cggtgctttc ttcttcctgc cgctaatggt cgcggcatct gccgccatca
    21661 aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt
    21721 ttattgaatt aatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc ctgatcccgg
    21781 tcaccgcggt gaaatacacc tacacggtga tcccggcgct ggtgatgacc tggtgcctgt
    21841 catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc
    21901 caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta
    21961 tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc
    22021 tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg
    22081 tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaagga atggtcatgc
    22141 catcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg ccgtggaaaa
    22201 cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg
    22261 ggatttccga accggcgtta tacggcgtgg cgatccgcct gaaacgtccg cttatcgcca
    22321 gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact
    22381 caatggcagc gccggggcta tttaccagcg tgcaattctt cgatccggcg aatccaatga
    22441 gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt gctgtcgttt atcctcacac
    22501 tgttgctcgg ctttgaggat attcctgttg aggaagcggc tgcccaggcg cgaaagcatc
    22561 agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt
    22621 atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt
    22681 ccgtgaaggc ggaaaagggc tgaccaccgt cgatatgatc ccacacggcg agcatcgaat
    22741 ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag
    22801 ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat
    22861 gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc aggacgatga
    22921 aatcacgccc aatcagcagg gcattgcttt ttatcgtcct gtctttgaag agtgtaaaaa
    22981 gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac
    23041 cgaatatggc tcctggcgta accgcaagct ggtggagttt ttcagccgct acgccagaac
    23101 ctgctttgaa gcatttgatg gtctggtgaa atactggcta accttcaatg aaatcaacat
    23161 tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga
    23221 tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccaccaaaat
    23281 cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gtaacttcta
    23341 cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg gagaaagatc gggaaaacct
    23401 gttttttatc gatgtgcagg cgcgcggcgc gtatccggct tactctgccc gcgtatttca
    23461 cgaaaaaggg gtaaccatag ataaagcgct gggcgatgat gagatcctga aaaacaccgt
    23521 cgattttgtc tctttcagct attacacctc gcgctgcgcc tcggcggaga tgaacgccaa
    23581 caacagcagt gcggcgaacg tggtgaaatc gctgcgtaac ccgtatctgc aagtgagcga
    23641 ctggggctgg ggaattgatc cactcggtct gcgtatcacc atgaatatga tgtacgatcg
    23701 ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc
    23761 caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat
    23821 gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg gctgtattga
    23881 tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg
    23941 tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa
    24001 aaaagtgatt gccagtaatg gggaagattt agagtaagta acagtgccgg atgcggcgtg
    24061 aacgccttat ccggcctaca tagttcgtgc gacatgtagg cccgataagc gcagcgcatc
    24121 gggcaattta gcatttggca ttagtcaaaa atcaaagaaa ccattccggc ttaacgccaa
    24181 atcgggctga aagtgcctta atgtgggata tcgtcaggga tcgttgtcca gacaaaatct
    24241 gactgaccag ggatttagag ccgatttcgt tcttcaaatc cgcataggtc agattatgtt
    24301 gatcaatcag cgtgcgaagt aacgccacgc caacaggcat tgccgcaacg gctttgtcga
    24361 actctgcaaa ctcttggttg ttgttttcat attctgcaat gcggctcgcc agaaagtcga
    24421 taagtggatt ggtgtcatct tcttcaatca aatattcgac cagttccagt gcttcacgat
    24481 aatcatcttc tgagtcacta cctcccagaa aggggactgc attgaccagt tctcttgttg
    24541 ctttaacagc gtgtgcagca ttagcagtca ttctttgttc tctctgtagt aacgtgtcag
    24601 tttgtcatac tcggcgtgat tagttatgtg cttcacaaag aaccgcttat taacaaagtt
    24661 tatgtaggca atcaccccta ggttgttccc acctacatcc agaacgtacc atttgttgcg
    24721 atatttaaaa ttgtcgaggt tgggaaacgc cgttcgcatc tcttctggcg tagaaaaatc
    24781 cgtttcctta ataacccgat acaatgcctg aagagccaga gcgtcgttag gatatttacg
    24841 cgcagactct tcaaatggcg ctttagaaat gatgtgcatt tttaagtttt aacttcctta
    24901 tagtaagtaa acagccacat ctgcgagttc tatccttatg tgttcggaaa ctatagctca
    24961 tcaaatccaa gttcacaata tgtaaacact tgtgcgcgat ataattaccc ccaaaatcct
    25021 catagccact ctgcaaaaag tcagttctgg ttcacccctc aagcacatcc ccctttatca
    25081 agcaacgctg cgtctgcgcc agatcttccg tttgcgggtc gcgcccggcg atgggtttga
    25141 ggacgagttt acggttgggg gtataagtaa aacgcgtgag cgcgggatag cttaacttgt
    25201 caaaccagcc gtgaacacgc tgccatttgg cgtcttttac ctgatacaga ctggttatcc
    25261 agtaaccaga attaaactgc atatccagca attgcgtgtg accattgcct tgcaaatcca
    25321 gcaaatcatc tacgccagtt ttgcttgcgg tgtagaatcc cctgggttca aagacacagg
    25381 gacggccttc gcgcgtgaac gtcataagaa ttaaatgtgc ataaggagcc agaccattac
    25441 cggtacttgg taaccagata accagatcct gaatgccgtt acgatcaaga tcggcgcgat
    25501 agagttgccc tttgcccgct atgtttgcca gcatacccac aggcacagtc caggatttac
    25561 cggcgcggtc tttagcggtt aattgccagt aatcttcttc atttggcatc ttctcaagcg
    25621 tcacgccagc aacgcccact ttcctaaaat gccaggtttg ctcggcatca taaggcatgg
    25681 tcaggagagg tttcagcgcc cgcttttgcg cgtattcgct gtcactcatt ggcagatatt
    25741 cggtaaagct ggtggcctgt gcaggcagca ctaagctcaa taaaaaagcg cacatccctg
    25801 ttctgatgtt caattccctg aattccctga attccctgat ggagaattat tcttcttcca
    25861 ccgctaactg cgcaaatcca ccatttcctt cccagccttc cagtcgttga taaacggttt
    25921 ctaccgcatc tttaatcggc tgggtcatcg ggtagtaaaa gccgacgata tccggctgaa
    25981 tgccgaggaa aatcacttca ccaatgtctt cttttaactg gtcgataagg taattcaacg
    26041 gcatgttatg ggtagtcatc ataaacatct cggcgatatc atccgggtcg atgatgcgga
    26101 tttcgccggg gtttagcccc atatccgtgg cgtcgacaat cagcagtcgt gtcgggcgca
    26161 gttcacggat ggcgacgatg tcgttttccg gtgcactacc gccgtcaatc accacccagt
    26221 tacctttcgg cgcggcggcg cacttttccg ccagcagcgg gccagcgcca tcatcgccca
    26281 tcatgctatt gccaacacag agtaaaacgt cagtcacgca gtctcctcac catcaaatag
    26341 atggcgtttt cctgatgaat atcatgcagc atacttaaca gcattgtact ccattcctgc
    26401 tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcgt tggccagcat cggcacatgg
    26461 ttaatgtcga tgacgatctc gccatatttc ggtacgcctt ccattttgtg gcgggcttcg
    26521 ctgcccgcct ccagagtggc aatccatgcc agatattcat cccatgggca ggtgagcgcc
    26581 gcttccaggc aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc
    26641 tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa atttacggct cagttgactg
    26701 aacaccacct tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga
    26761 caatctcgtt cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc
    26821 cctgccctaa ctgtgtaagg taatcatcgg caatctgacg accgtaacga taacccgcca
    26881 ggcgacgtgc ttcgcgatcc actttcacgc gcagcggctg caccatatcg ccatgcagga
    26941 tctccgccgg ttgttcatcc agttcacccg gcccacgggc gtgaattttc tgctccagca
    27001 ggccgagcgc cattgcaaag ccgtacagcg tggcggcagg cgttggcggg cagctaggga
    27061 tataaacatc cacagggaca attttatccg taccgcccca cacgcagtag agatcgtgta
    27121 agatcccgcc gctgttaccg caggcaccgt atgagataca gattttcggg tccggcgcgg
    27181 actgccacgc acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta
    27241 aaatatccgc atgacgcggt gaaggaacga ctttaatgcc gaagcgttct gcatcgaaca
    27301 gcggagaaag cgtggcgaaa atttcgattt cgcagccgtt gcagccgccg cagtccacgc
    27361 ggtaaacata ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg
    27421 attcatccac cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc
    27481 tcatcagatg gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca
    27541 tttctggcgc ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct
    27601 gtcaccattg tgcttaagca gcgcaatggc gtagtcgatc tctttctgta cggcgaaagg
    27661 acgattgcag acgcggcagt tgcacagcgc gaagcgggac tgttgcagga agtcttcttt
    27721 cttccacacc gccagttcgt actcttgcga cagtttgatc gccgccgtcg ggcaaacttc
    27781 ttcgcagcgt ccacagaaga tgcagcgccc gagattaaac tcccacgcca gctctcctgt
    27841 ggcgaggtca gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aagccgcgca
    27901 gccgatgcac tgctgcgggt tctgctctgg cttaccacgg aagtttttat caaccgcaat
    27961 cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
    28021 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
    28081 ttctttgtac ggcaccactt tgctcttctt cttacgcaca tcgactacgg tcatgcggtc
    28141 ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatcgg aaacggtatt
    28201 gccacgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
    28261 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc
    28321 ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
    28381 accgcccggc aggttatcca gaccgtagtc gatcatattc agcgcggtat agacctcgtt
    28441 gattcgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
    28501 cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga gaccgctggc
    28561 gcgaaccatc gggccaacgt tactgaaatc gcaggcgatt tccgggtcca gacgaccaat
    28621 gccgacggtg cgttgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
    28681 ttcacgacgc atttgttgcg ccagctggcg ggtctggatc atgtcatctt tcagcagatc
    28741 gtgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc
    28801 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc
    28861 ggtaaagtga caggcgaggc cgaggttgag cagatgcgag tgcaggcgtt ccacctccag
    28921 cagaatggcg cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac
    28981 cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag
    29041 gaaggtcact tcgttataac ccatgcgggt ttccgccagt ttttccatac cgcgatggac
    29101 gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
    29161 acccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
    29221 gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg
    29281 ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagacc
    29341 aaccggaatc aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac
    29401 gcgcggcgtc acggacggat actccggttt gttggcgtca acttcgacgc gaaccgttac
    29461 ccaacacttg gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag
    29521 tttgcgttcg tcgttaccaa acagcaccga cagccagcca ccctgcttgt agtagagaaa
    29581 ctccaccact tccggcagat agttcacctt tacggtgaca gtcagctgat ctttggtctg
    29641 ccaggcgtgg tccagcacga cgcctggaaa tgcctcattc agcgcggcga gataatgttg
    29701 acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg
    29761 aacgctaaaa atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc
    29821 agacgggcca tactgttttc gaacagcgcg ataaccagga cgccgaccac cagtttgacg
    29881 atggcaatca ccagcgccag cagcagtcca ccgacggtga aggtttccat ttgtccccac
    29941 ggaataaaca ccccgacgaa catctgcaac accaccagct gtttcaggct gataccccat
    30001 ttcatgacgc caaagccgct accgctgtat tcggagagcg gaccttcctg caactcctgc
    30061 tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag
    30121 gcgcagagcg ccagcaccag cgggatgctt tggctcagcg gccagtgata aacggtgtcg
    30181 gtaatgttgc tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc
    30241 aacatcggtt cgaccagcac gccaagcatc gcttcacggc tcgcaccgat agcggtaaac
    30301 gggctaccgg tatccagacc ggaaatggca aagaagaaac gcgcgatggc gaagagatac
    30361 agtaaggtga tcaaatcgcc cagttgcggc agcggagaac cgatagtcag catgacgccc
    30421 accatcacat acggcgtcag gcggaacatc cagccagagg catccgggcc aacgctctgg
    30481 cggcccagca gtttgataat gtcgcgatac tcctgcaaca cgcccggccc gcgacggtta
    30541 tgcagacggg cgcgcgccac gcgggtaata ccggagagca gcggcgcaac ggcaaataac
    30601 accagcgcct gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa
    30661 tcaccaccag taccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactacctt
    30721 cgcactgcca gcccggcacc agcgacaccg gattcagcca tttgcgcagt ttcagcactg
    30781 gcgcaaacgc ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt
    30841 catggtcgta gccgcacacc caggccgcac cgcgggaacg tgacggcagg cggtcgcctt
    30901 tgcaaatcgc cataatgatg aatggcagca gcgggcaggc aatcagcagc aatgtgatca
    30961 ttggttgaga aacggtggtg ttagcaggct ccagaggcag aggtacagca gcagagagca
    31021 tcggcagcag ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta
    31081 cgcttacgct catcaggagc ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg
    31141 cgccgaggaa cgtgacgcca tagactttcg ccatacacat caccgccagc gcaccggtaa
    31201 ttgccagacc cacagcgagc agcggcccca gcagacgggc aacaaacgcg ccgctattgc
    31261 tcagtttgaa gaaggattga tagataaccc attccccggc aaacccgttc agtggcggca
    31321 gcgcagccat tgccatcaac ccgactaaca tggcgatgga gataaccggc attttcttgc
    31381 caataccacc gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgccc
    31441 cgaggaacag tacgcttttg aacaggctat ggttaagcag atggtaca
//
LOCUS       DADLXX010000033        30681 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLXX010000033 DADLXX010000000
VERSION     DADLXX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.033"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="JNX27_001540"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="JNX27_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HAY7882790.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="JNX27_001541"
     CDS             complement(1899..2636)
                     /locus_tag="JNX27_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882791.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="JNX27_001542"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="JNX27_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HAY7882792.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="JNX27_001543"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="JNX27_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HAY7882793.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="JNX27_001544"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="JNX27_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HAY7882794.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /locus_tag="JNX27_001545"
     CDS             complement(6912..8285)
                     /locus_tag="JNX27_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HAY7882795.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="JNX27_001546"
     CDS             complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="JNX27_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HAY7882796.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="JNX27_001547"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="JNX27_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HAY7882797.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="JNX27_001548"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="JNX27_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HAY7882798.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="JNX27_001549"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="JNX27_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HAY7882799.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="JNX27_001550"
     CDS             9819..10154
                     /locus_tag="JNX27_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HAY7882800.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="JNX27_001551"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="JNX27_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HAY7882801.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="JNX27_001552"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="JNX27_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAY7882802.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="JNX27_001553"
     CDS             complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="JNX27_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAY7882803.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13339)
                     /gene="traN"
                     /locus_tag="JNX27_001554"
     CDS             complement(11510..13339)
                     /gene="traN"
                     /locus_tag="JNX27_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAY7882804.1"
                     /translation="MVFFLSFSVFADSNSDYRAGSDFAHQIKGQGSSSIQGFKPQESI
                     PGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAP
                     FIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRTASITGDW
                     KYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFM
                     NTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLV
                     SGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKP
                     WNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSC
                     ESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVAALNGVDV
                     RAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFC
                     SRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQKI
                     QFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="JNX27_001555"
     CDS             complement(13650..14030)
                     /locus_tag="JNX27_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882805.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="JNX27_001556"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="JNX27_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAY7882806.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="JNX27_001557"
     CDS             complement(14674..14979)
                     /locus_tag="JNX27_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882807.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /gene="traU"
                     /locus_tag="JNX27_001558"
     CDS             complement(15009..16001)
                     /gene="traU"
                     /locus_tag="JNX27_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HAY7882808.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..16630)
                     /gene="traW"
                     /locus_tag="JNX27_001559"
     CDS             complement(15998..16630)
                     /gene="traW"
                     /locus_tag="JNX27_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAY7882809.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="JNX27_001560"
     CDS             complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="JNX27_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAY7882810.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17010..19640)
                     /gene="traC"
                     /locus_tag="JNX27_001561"
     CDS             complement(17010..19640)
                     /gene="traC"
                     /locus_tag="JNX27_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAY7882811.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19766..20113)
                     /locus_tag="JNX27_001562"
     CDS             complement(19766..20113)
                     /locus_tag="JNX27_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882812.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20141..20359)
                     /locus_tag="JNX27_001563"
     CDS             complement(20141..20359)
                     /locus_tag="JNX27_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882813.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20458..20931)
                     /locus_tag="JNX27_001564"
     CDS             complement(20458..20931)
                     /locus_tag="JNX27_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882814.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20924..21337)
                     /locus_tag="JNX27_001565"
     CDS             complement(20924..21337)
                     /locus_tag="JNX27_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882815.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21330..21551)
                     /gene="traR"
                     /locus_tag="JNX27_001566"
     CDS             complement(21330..21551)
                     /gene="traR"
                     /locus_tag="JNX27_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HAY7882816.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21686..22201)
                     /gene="traV"
                     /locus_tag="JNX27_001567"
     CDS             complement(21686..22201)
                     /gene="traV"
                     /locus_tag="JNX27_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAY7882817.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22198..22566)
                     /locus_tag="JNX27_001568"
     CDS             complement(22198..22566)
                     /locus_tag="JNX27_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HAY7882818.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINVITARVVPDKNVSDDFMHAVL
                     SNCTTKIVLPAPKVFSSESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKT
                     NLTAELISAVVGSNKKGEGQ"
     gene            complement(22505..23095)
                     /gene="traP"
                     /locus_tag="JNX27_001569"
     CDS             complement(22505..23095)
                     /gene="traP"
                     /locus_tag="JNX27_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HAY7882819.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23085..24512)
                     /locus_tag="JNX27_001570"
     CDS             complement(23085..24512)
                     /locus_tag="JNX27_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HAY7882820.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24512..25240)
                     /gene="traK"
                     /locus_tag="JNX27_001571"
     CDS             complement(24512..25240)
                     /gene="traK"
                     /locus_tag="JNX27_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAY7882821.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(25227..25793)
                     /gene="traE"
                     /locus_tag="JNX27_001572"
     CDS             complement(25227..25793)
                     /gene="traE"
                     /locus_tag="JNX27_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAY7882822.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(25815..26126)
                     /gene="traL"
                     /locus_tag="JNX27_001573"
     CDS             complement(25815..26126)
                     /gene="traL"
                     /locus_tag="JNX27_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAY7882823.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(26141..26500)
                     /gene="traA"
                     /locus_tag="JNX27_001574"
     CDS             complement(26141..26500)
                     /gene="traA"
                     /locus_tag="JNX27_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAY7882824.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(26534..26773)
                     /gene="traY"
                     /locus_tag="JNX27_001575"
     CDS             complement(26534..26773)
                     /gene="traY"
                     /locus_tag="JNX27_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HAY7882825.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(26881..27528)
                     /locus_tag="JNX27_001576"
     CDS             complement(26881..27528)
                     /locus_tag="JNX27_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HAY7882826.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(27720..28103)
                     /gene="traM"
                     /locus_tag="JNX27_001577"
     CDS             complement(27720..28103)
                     /gene="traM"
                     /locus_tag="JNX27_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAY7882827.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            28436..29026
                     /locus_tag="JNX27_001578"
     CDS             28436..29026
                     /locus_tag="JNX27_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAY7882828.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(29323..30144)
                     /locus_tag="JNX27_001579"
     CDS             complement(29323..30144)
                     /locus_tag="JNX27_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY7882829.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(30255..30551)
                     /locus_tag="JNX27_001580"
     CDS             complement(30255..30551)
                     /locus_tag="JNX27_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882830.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
BASE COUNT         7777 a         8121 c         6910 g         7873 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatcgcccc
    16561 aggtgccaag atcggcggcg gccacactct gcccccatat cagcagggca atcagccccc
    16621 ggcatctcat ggttccgctc tcattcgccg ggcaatatcc tgctggattt cccgggtgat
    16681 atccggagcc ccctgtacca cggcaggcga caccagaatg accgcgtggt gtttctgctg
    16741 ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc gctgaaaggg cttttgactg
    16801 tgcttctgac agttgtttct gactggcgct gtcaaaaaat gcatccacag tctgtttcat
    16861 gttgaaggcg gcagtcaccg gtgcgttcag tctgacaatg ccgtaactga cggctgcgtt
    16921 cagtaacacc attgccaggc atcccggcac cgtccaccac cagtggctgc gtcggggggc
    16981 tgtaacatca gtcgtttttt gcgttgtggt catgcaacac tcctgtattt ctcatgttct
    17041 tccagccagg cttccagcga agccatctcc ggccctgact tcttccacgc cagctgccac
    17101 accgcctcat gaatactcag gccctccctg cgtttctgct gcacaaactc aaaatccggg
    17161 ccgtcagagc tgtacatggc gcggcttaac gggtccacaa acagacggtg ccaggaggaa
    17221 tggttttcca cctgcagcag gaaggaactg aaccactggt ctttggccgc accaaacttg
    17281 ccaatcatgt cgcgctgcag tggctggaac tgatccggga ataactggtt atatttcgcg
    17341 aactccttcg cactctgttt gaggataatt ttgtaggagg agttacccca tgccgcacgg
    17401 gccgcactgg aggccttgtc agagtcaaag tcgacgatgt tctgtgtgat ggtgatatag
    17461 gcaccggtat ggcggcgaca ggtgcggtaa cctttttgaa taaactcacc gaccttgcga
    17521 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gagagtacgc
    17581 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
    17641 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
    17701 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
    17761 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
    17821 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
    17881 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
    17941 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
    18001 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
    18061 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
    18121 gagtccagca cgctgcggat aagtggctgt atcagtccgg ttttaccggc cccggaggtg
    18181 ccacagaccg ccatgttgta gttggtgtta ttcatcccct taaagaagat atcgataaac
    18241 gccagctggt tacggtaggt gggcgccagc agacctgccg gtgtcagggg gttatccgcc
    18301 actaacggca tcaggttggc cacattaaag ctctctgcgc gctgaaccac accggcctct
    18361 ttcagctgtt taaacagccc tttcccggcc ataaagggca ggcaggtcag gaaattgcgc
    18421 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
    18481 tcctgctcca cttccagtgc cgtttcatta ttgtccttgc agaaggctgt gatgttgagg
    18541 aagtaggaca cgacagaaga ctggccggag cccagccgct ggcgcagttc cccccactcc
    18601 ttcgcctctt tctccacgga cggaaaccat ttggcatatg aggtcttcga cttcttctcc
    18661 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
    18721 gtcagcgtca ggatgaacgg gcaggaaatg gacatttccg ggttcagcag attgctgtag
    18781 ttgtcggcca tgttccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
    18841 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
    18901 acattcaggt caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
    18961 tgacgtcttt tcgggtacag agagtccgga ttatggttaa tcatctcccc gacaatctcg
    19021 atgaaagcct gtgcatccac tgtctgtgtg gtgatttttg cgccgtgaaa agaggcacgg
    19081 atgattttca ccagattttc catttccaga atgtcagccc ggcttttttt cttcgaggga
    19141 gaacagtacg agataaacac ccggtaatga cgcagggtca ggggcagatt catcccctcc
    19201 ggcagcggga actgtgtcgc tgccgctttc atataatagg cacgggtaat ggcgttaaac
    19261 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat cctgtcacca
    19321 accagctgac tggacatcag atggatacac aacggaatac cgcgcggcag tttggtgcgc
    19381 agcatgtgat cgagtgcctc cacaatggac ttattcgccc cattgatggg aatggcttcc
    19441 agcataaagc ccatcgtggt gtcattcatg aacagaccgg attcctggtt gtaatcacgg
    19501 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
    19561 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
    19621 tcaagtgggt tattactcat atgagtattc cttagtgcgt ttaaaaaagg aaaatatcag
    19681 tctgtggccg tggatatatc acgcagtgcc attcctgaaa ccacagcaca aataagagat
    19741 ggaggctttt aacaggaaac caggattaac gttcctttac tgaatgagca tcatttaatg
    19801 cctgaatttg tttttcgcga atcttattgc gctcattttc tcttacccgt ttcacctctg
    19861 ccatttcgtt tctttttctg cattctgatg cgattttatt tgccttacca agtgttaaag
    19921 gttcagactg ataattaagt gtctcctgaa atgcttctgc tgcacaggga gtttcctgta
    19981 aaatctctgt cagttgttct ggtgttgcag gttgttgcgc aaggtaaacg atgtaaaaag
    20041 gaatacatac aatcatagcc ataacgccaa caaatataaa agccagaaca gattttaaat
    20101 atatcgactt catttcattt tccttcgaag cagtaagcac tcattgccca ccaccattta
    20161 gtgtttccat cacgctgttg aaaggttcat ttgttttaac ttcaatacac tcccctgtat
    20221 ttccaaagcc cctgccgcca tgtgcttcga ttgtccgaag aacctgcacg catacgtccg
    20281 tatttggcat gggtgtaata ttccagcttc cgccaccgtt aatatttttt atcagcacaa
    20341 gaaaaataaa ggtatgcatt ctttattatc tccgcttgta tattaatcac tgaatatccc
    20401 ttcagttaac ttatgaggtc ataaaacact gcagattttc attcagattt actctcttca
    20461 gcaaacttta ctctcttcag caaacttccg gatggtatcc tgttcacgct gacgggtggc
    20521 agactcctgc attgcatcca ctttctcctg ccagagacgc cggatatcct tttcatcccg
    20581 accagtcagt tttttaccgg acagcagacg gttaagcagt tcctgtctcg catccggtac
    20641 gtcttcagtc agacgcataa gctgaagcag gctctgacgg ctgagaacca tgagtgattc
    20701 cggcaggagg gtgaagaaga aaataaaaaa gcataccgca ccacagcagg caaggaccgc
    20761 aaacaggatt ttcagatata taccatcaac gccgaacgca gcaataatac agggaatcca
    20821 tgcaatacac gcacaatacg ccaggatgtt atctattcgg ttatgtctgc gatatatctg
    20881 ttgatgttca tccagaatag aatctatttt ttcagcttct gatttatgca tagtgtttcc
    20941 tctgcgtttc ctgccagaga cgccggatat cctgttcatc ccgaccagtc agttttttac
    21001 cggacaacaa acggttaagc agttcctgtc tcacatctgg tacgtcttca gtcagacgca
    21061 taagccggag taattcctga cgactgagta ccatgcgtga ttcctccggt acgctgtaat
    21121 aattaaaaat acgactgaat accccacaac agcaggcaac ggacaggcac agggatttca
    21181 gatacaggcc agaaacacag aatgtggcaa caccacaaag aatagctata actaaaaatc
    21241 ccttacccag tgccatacac aaagaaccct gttttctgga tatccgctca tgctcatcca
    21301 gaataaaatc tattttttca ttttctgatt tatgcatagt gtttcctctg cctttcctga
    21361 tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg ggcttccgga
    21421 ataggatttc cgcatgcttc acagagataa acaggaatac catgaacatt tatttcctgg
    21481 cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc ttcatcggct
    21541 tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc atcccttttc
    21601 acgcacaggg cactcgtttt acaggcggat tttgaataca gaaagcccgc cggcattgcc
    21661 ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac gcagacggtt
    21721 taataacaaa aaacacgctg gatggctgat gataaacatc ctggttatca atataaggcg
    21781 ctatccataa tgctgccgtc tgctcacccg ttcttagcgg acgaggaggt gttaccggcg
    21841 taactgaact gaccggaaca accgttttca cttcccgggc agcggtaaac agcgggcgtg
    21901 gtgccagcag tttctgctcc ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg
    21961 tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg
    22021 atgccgcaac cggctttgct tctgaggact gctccagttt tttggccttc tcattggcct
    22081 gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc gtatttgttc
    22141 ctgcgcagcc ggaaagtaat aaggttccca gcagaggaat aaataaagaa atctgtttca
    22201 ttgcccttct ccttttttat tactccccac aacagcagaa attaactctg ctgtcagatt
    22261 tgttttccct gcacagggat gtccggagac cactgtattt cccggagaag aaaataagct
    22321 catcgacatt cccttcttca tcacaccgac agcagccata ttaaatttgt gcggcaatga
    22381 ttcagaactg aacaccttcg gagcaggaag aactattttt gtggtgcagt tactgagaac
    22441 agcatgcata aaatcatctg atacgttttt atccggcaca acgcgggctg taataacatt
    22501 aatattacgc atgttcattt ttatctccgt tgcttctgtt tgttcggtca tcagggaaat
    22561 aaacatggca tttcctgatt tttcccggca gggaagcgtc ttccgtataa acgtccttca
    22621 gatattcatc tgcggttacc acccggatac atttcatttt tccgggcagc accggatatt
    22681 tcccggtgat cgctgccagc gagacataca tcagtgccag tgttgcccat aatgcggtga
    22741 ccagttgcat cagtgaccgg tcagtctccc tgacataatc tgcagcatca gccgcgcggt
    22801 aatggcagat atcctgtatt tctgatctgc ctgaagaatc agaggaagaa aaccagcatt
    22861 cactgaccgg gaactgtccg gcaggtgctg tttgtcttac gaagttaatc tccttaacca
    22921 gtaatttccc cggcgtggtg ttgtccttcc ataaaacaaa cagagccatc agggccattg
    22981 tggccagtgg cagaatgacg gtgtacttca gacaccagaa cagtcctcgc agaacccggg
    23041 cgacaacata ccgggcggca tggcacgcct gacgggacga catattattt gccatcgttg
    23101 cctcctccct ggctgccgga cataaactgc tgcggattca ggttgcccag ctgcttcagc
    23161 atctggtcag tattaaagcc gttaagatga ctttcagctg atggcggaac aggaaccgga
    23221 ctttccggat tatcttcttc cgctctgttc tgcgtgcgct ccagcgccat ctcttccacg
    23281 gttttcagct ggaagccgtc ctggaacacc acggtcactt cgttgcccgc accaatcggt
    23341 atcaccgggt gatactgttc ggcacgtttg atgtagtagt cactgagcgt ctgtgcggct
    23401 ttcgatgtgc cgccaccgat acccattttc aggacatcac cagccccgta agcggctgtg
    23461 gcacccagcc cgacagccgg ctgggaggcg cgctccattc cctgaccgat accgtcaaca
    23521 aatcccgcgc cccatgccca gccgaggatt ttgccgttac gcatcaccac ttcgcccttg
    23581 ataccgtttt taccccggaa gctgacatgc cccttaatcg gcatatcaat agttttgccg
    23641 tccttcaggc aactgatatt gcgggtgcgt acaatggcac gctcactgga cacatccccc
    23701 caggcttcca gccccacaaa acatcccgtt gcgtcatacg tcttgctgtt cggcatttcc
    23761 accaggccgg tgatacgcaa ctgcatcggc accgtggatt cattaccggt gactgaggca
    23821 ttggcatccg ccccttcaat cagcatggct ttcgcaaaac ttcctgacgg aatgtacggc
    23881 agcgatggtc cctgttttcc ctcattacgg gtaaacacct tacgctgtat ccggttaggc
    23941 accggcacag actggtacgt cacctgtggt ggtggcgtga caccattccc gggataaaac
    24001 gccgtcggcg gtggaacagc aacgctgccc tgcggcggga aggatacggg ggtgtttcct
    24061 ggctgtggtt cgccttccgg cccgggcggt gaggcaggca tctgaggtac aggctcaccg
    24121 gtcgccgtga cgggattagc acccagggct tttacctgct ctgccagggc ggcattgtcc
    24181 tgtcccagct tttcaatacg acgctggtca tcaccgcgct gtttgttcag gacatccagt
    24241 tcacgacgga tttcctcata ctgcttctgc atctgcgctg ccgtcacctg catctctgtg
    24301 gtggcatgct gacgcacctt gtcatcaaag gtcgtatcca ccacacctgt catatccggc
    24361 acaggctcct gttcagccac cgcctcaccg ttaccggaca tgtccacatc agacagatac
    24421 agtaccccac caatcgcgga ggctgcaccg acaaccacaa tccccagcca caggtactgc
    24481 ttgcgtttca caatggtatt gatactggcc atcagttgcc ctccccgtca cggatgacat
    24541 acacatccat gcgcgcacct gccagtaact ggctggcagg ctggctgaac atcacggcac
    24601 gggttcccgg ctgccagaag tcactttccc ggatattcag ggcagacagc gttttatttt
    24661 ccacggcaaa gcggaccatc ttcaggtgat tccccgtcca tacggcatct gccactgaag
    24721 acagccccgc cggggcctgc agggtttcct ttgtcacagg aacctgatac cagcctgcag
    24781 gtaattttcc gccacggaca gcctggctga tggtcacaag cagggattcg taaggcgtgg
    24841 acgtttccca cgctccggct tcttctccgg ttccgcgcag gtcactgacc agctgaatgg
    24901 tacgccccgc gccttcacgg ggaacagcct gaatggaaag gttcagacca cgctctgttt
    24961 ccagaatgaa cgtaaagggc tttttgttga cggtggccac caccacaccg ccggaggcgg
    25021 tctgctcatt attggtcagg gcaccatcca gactgttcac ggcgataatc cggtcaccgg
    25081 gaacggtaaa cagattcggg ctggtattgc tgatggcaat actggcctga ccaccgttca
    25141 ccattggcac cacagtgggg gccagcgtac cgtttgcggc catcacgctg caggaaaaaa
    25201 acaggccgcc gaatattatt gccgtattat tttttctcat cgtctgtttc cccgaaatta
    25261 tccagccagg tcaccccgtt ttcccgcttc agaataagga tgtaatgttt gatatcagta
    25321 aaaggttcgg agttaccaat ccatgttttc cgaattccac ggatttccac acgaccatac
    25381 tgaggccaga cacggacact ggtctggaaa aaagcagaat tcacgttatc ttttttaatg
    25441 agtttcgctt cttcagccag aataactttc atctggttct gtgcacccgg gcggatatat
    25501 tgcagaagcg cctgatgtga agcatcgacg atttccggtg agacattcag acggagggca
    25561 ataaatgaca gcgccatctg ctgcaaataa gaggcatcgg cactgttctg tgacacggca
    25621 aagggcacat taaatcccat tggggtcacg gcagtgcgtt gctcattctg aagacggtag
    25681 ttattcaccc cctgaatgag gttaacagag aggctgagaa caataagcac tgacataaat
    25741 ataaaggcga tggccattac acgactggta cttaaacggg caccgtgttc catataacaa
    25801 tcctggtatc agttctattt aatccactgc cggaaacacg aatcgggaac attatgaaaa
    25861 ataccgcgca gcagggctgt tggcatatac cagtaaatca ggtcacgtaa ccaggaactg
    25921 ccccgccctt ttttcagttt tttaatcccg aaataaacca gaaccgctgc accaataccg
    25981 aacagatatt tcgatgttgt gataccccag ccaacacaga ttgctgcggg gatcagttca
    26041 tccagtggca ggccaaacca gcggctctgg ttggtcagtg tttccgggaa acgatatttc
    26101 ttaagtttat tctcgtctcc cgacatcgtt ttatttcctg tcagaggcca acgacgctca
    26161 tacccacagc aataaataca gagatgatgg caaagccgac caggaacttg acgtttttgg
    26221 tcatcatgta catgaccgca ccgaccagca cctcagccag aacaacccat ttaacgatac
    26281 tggagtcctt accgaaggtc gccttaaccg tatcgttacc tttagccatc aggtcgcctt
    26341 taccagcagc catagctaac tcagggaaca acatcatcag aacagcaacc gggatcactg
    26401 cacgaaccag gcgaagcata ttcagacgag tgaatttaga aaaaaacgat ttattttcaa
    26461 cgggcgcaga agcaccctga acacttaaaa cagtattcat atgattcctc tgattaatat
    26521 tgacagatga aaactaaagt tctttaaatg ttgactcgtt ctcttcgatc acctccctga
    26581 atatttcttc attataaaaa tctggataac gttgcaaatg atcctgcaac ctcaccaatg
    26641 cttcgtttgt tttcgttctt ccactacgct ctctggctcc aagtaataat ttattcgttt
    26701 cctcatcaag ggtaagtaaa acttttctac ttggcgcaga tcttaaagct cctttaatca
    26761 ccctccctcc cataaataaa agagatttag tcaaaatatt cacataagac gcacacaaaa
    26821 ataaacaaca aaatagtaaa ttacgttaac aaaaaccggc acttttagtg ccgggaatgc
    26881 ttacatttct ttacagtgaa ggctgttaat attactagcg aagtattgat atagataaaa
    26941 taacacatca gagattatag catatcgcaa ccactcatcc ctgtctgaga aaccatagtg
    27001 ttttttaaaa ttcataaata tttttttaga ttggtcaaca gagatatcca ttctctgaga
    27061 agaatactca tgactataac ccaaaagata cggtattatc ttattaaagc gatatttgtc
    27121 aggttcacaa ctaaactcct tgccctttat aaaaaagcca ctaactacgt atctttcaaa
    27181 gaatctccag acaaacaaat tcaagttatt cacttccgta ctttcaacca ccacatccca
    27241 tgatacatca ttcaatatta caccattaat aatactcact ccattcgagg acgagaaagc
    27301 atcaacttca gcagtatgaa ataaaatttg cgtatcaata tcaataccag aaaaccatat
    27361 ttcagaatca ctattctgac actttattaa gtgtaaaaaa acagagtttg agtctatgaa
    27421 ttttcctgac tctgtgcgga cacagactgg atattttgcg gataaaataa tttccggcag
    27481 gcctgattga cgtatgagtg ctctttctct agtatccatc gcacacatag gaacctcctc
    27541 aaaggattct atggacagtc gatgcaggga gggacaagct ccctgcatcg gcgattttca
    27601 agttcaaatt ttaacgtggc attaaccacg taattaatat actaattatt ctgattattg
    27661 tcatcagttt gaaccattaa aaagtctgat atttgaacaa agtcaaaatt tatttccctt
    27721 tattcatcat cattttttgg aaagaacctt tccatctcag ccgacacctt ctctctgata
    27781 tcttcaacca tattggcata ttcaaacttt ggatttcctg agacatgagg actgagagac
    27841 tcaataccca aaattttcgc tactgatgac tgtgttttta caacgcattc aagaagtagt
    27901 ttattaaact cggcctgatt aaatgcagac tctttgcgct ccatctgagc ctcatataca
    27961 cgaagaccca gttcaagaag cattgaagcc gttgctgaaa aactgacatc tttttctctc
    28021 gccccctcct gtcgacgttt ttcaataatc gcgtttattt tttcataagc atcattgctg
    28081 atatacagat tcaccttagc cattagatat tacctttttt aaacataaaa caaagttaat
    28141 ttcgaaacac tagaattcat agtgcaaaca gggacgcacc gctagcagcg cccctgacgg
    28201 tatcctataa aaaaacacac cgcgccgcta gcagcacccc taatataaaa taatgttttt
    28261 tataaaaata gtcagtacca cccctacaaa gcggtgtcgg cgcgttgctg tagctgcgtt
    28321 aacgacgctg ctttaaaaaa atcagattta aacaatataa atccacaaat acaactcaat
    28381 gatattaaag ataaatcagc aaaaacttgt ttttgcgtag tgtgtggtgc ttttggtggt
    28441 gagaaccacc aacctgttga gcctttttgt ggagtgggtt aaattattta cggataaagt
    28501 caccagaggt ggaaaaatga aaaaatggat gttagcaatc tgcctgatgt ttataaatgg
    28561 gatctgcgaa gccgccgatt gctttgatct tgcaggtcgg gattacaaaa tagacccgga
    28621 tttactgaga gcgatatcat ggaaagaatc ccgttaccgg gttaatgcca tcggtattaa
    28681 tccggtaacg ggatatggca gcggactgat gcaggtagat tcccagcatt ttaacgaact
    28741 ggcccgctat ggaattaagc cggaacatct gacaacagat ccctgtatga acatttatac
    28801 cggtgcttat tatctggcaa tagcctttaa aaaatggggc gtctcctggg aggccgttgg
    28861 tgcatacaat gccggattca ggaagaccga acgccagaac cagagacgtc ttgcctacgc
    28921 atcagaggtt taccggattt atacctggat aaagagcagt aaaggcatcc gggttccgac
    28981 cacgaagaaa tcactttccc aaattaacag tgtgcagaaa aattaatatt gtatacattg
    29041 tcaaataccg gttatgccgc gacagcggca tggaaggcca cacacaaaaa tatcatatga
    29101 gtgataatgc tgactggccg aagccccgca aagcgggatc ccatcttata tatatccggt
    29161 aactgcgcag cctcctgcca gaccattttt cgcgggcaca atatttacag actaccgaaa
    29221 agccagttga tgaaattgag gcatatcaga cgacacagca atgccgcagg atgataatat
    29281 aagtaaagcc ccgaaaaatt tttcggggct ttacttatat tatcatgact tccactccct
    29341 gaactgttcg gcaatcatcc ataatgcttt gttgattcgg atatcaccgt caatgcctgt
    29401 aatggctctt gttctggtat ttttaccgga ggcacttcgc cccgataatc caccttttat
    29461 catattctcc tgaacccgct gccaggttgt ccagagatcg ttctgtttat cctcccaacg
    29521 gcggggagta attatttgtt ctggcgtcac tggcgtttta ttttcatctt cgtacctggc
    29581 catcaatgca gctctgccaa ataaatgttg ttcgtcactg ttaagatgta tctctttcat
    29641 tgtctccata ttttcagtga ctttatcaaa gacaccgagc acttcatacg ctccctcaat
    29701 aacctgcccg acaatatcac ctttatgtgg aacgcggatt tcgccaaaat tattcccaca
    29761 caccaggcca tttgtgcaga caaaacgaaa aattcctggg atcatctgat aactggatga
    29821 accatcatgt gaattaagca ggataatttc aggaacttct ttcccgttaa tatgcccttc
    29881 cctgcgaaga cgtaacatat gtttactgta ttcgcggcgt cccaaatcac gaacccgact
    29941 ctgacaggca aagaatggct ggaaaccttc atcacgtaac ttattgatga tattgatggt
    30001 tgggatatat gtataacgct cactccggga ttcatgttta tcaccagaaa agacagatgg
    30061 aacaaactgc attaattcgt catccgttaa cgggcgctca cggcgaatag aattatatcg
    30121 accaaaacgg gaagctaatc gcataacagt aactccttta tgaaggcata aaattaataa
    30181 aagttttttc gcttaatatc atcccccctc ggttatcgtt aacaaaaaga caacaattcc
    30241 gaggggaagg accatcaaat caggtcagtt cgggactgac caacctgccg agtgaaaagc
    30301 agtaacagcg cgatcaactc atcagcgttt tttctgacaa gaggagtatg attctccagg
    30361 ctatccagta agtcatgcag ctcattattg acctggtgga gcaccttaaa gagtgcacca
    30421 gaatcccggc agctctgata tgcaagattt gcctgaacag cacgattctg catgatcccg
    30481 atttgtgcga ttgcgtcttt ctgctgatcg cctgttggct gataagtggt gttaaaagaa
    30541 aatgcgctca taattcctcc tgtatctgat gttttcagcc cccatcatct gttgagtttc
    30601 gggagccgcg gttttgacgt tttgcgggga cctgcctttt gttccctccg gtattcacca
    30661 tcacctgttt gagaaccttc g
//
LOCUS       DADLXX010000034        30537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLXX010000034 DADLXX010000000
VERSION     DADLXX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.034"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..904)
                     /locus_tag="JNX27_001581"
     CDS             complement(<1..904)
                     /locus_tag="JNX27_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY7882831.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAP"
     gene            1199..1873
                     /locus_tag="JNX27_001582"
     CDS             1199..1873
                     /locus_tag="JNX27_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7882832.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(1912..3573)
                     /locus_tag="JNX27_001583"
     CDS             complement(1912..3573)
                     /locus_tag="JNX27_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7882833.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(3769..4197)
                     /gene="ibpB"
                     /locus_tag="JNX27_001584"
     CDS             complement(3769..4197)
                     /gene="ibpB"
                     /locus_tag="JNX27_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7882834.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(4309..4722)
                     /gene="ibpA"
                     /locus_tag="JNX27_001585"
     CDS             complement(4309..4722)
                     /gene="ibpA"
                     /locus_tag="JNX27_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7882835.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            5027..5359
                     /locus_tag="JNX27_001586"
     CDS             5027..5359
                     /locus_tag="JNX27_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7882836.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(5361..6534)
                     /locus_tag="JNX27_001587"
                     /pseudo
     CDS             complement(5361..6534)
                     /locus_tag="JNX27_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            6599..7617
                     /locus_tag="JNX27_001588"
                     /pseudo
     CDS             6599..7617
                     /locus_tag="JNX27_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(7578..8915)
                     /locus_tag="JNX27_001589"
     CDS             complement(7578..8915)
                     /locus_tag="JNX27_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7882837.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(8990..10138)
                     /gene="dgoD"
                     /locus_tag="JNX27_001590"
                     /pseudo
     CDS             complement(8990..10138)
                     /gene="dgoD"
                     /locus_tag="JNX27_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(10135..10752)
                     /locus_tag="JNX27_001591"
     CDS             complement(10135..10752)
                     /locus_tag="JNX27_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY7882838.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(10736..11603)
                     /locus_tag="JNX27_001592"
                     /pseudo
     CDS             complement(10736..11603)
                     /locus_tag="JNX27_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(11600..12289)
                     /gene="dgoR"
                     /locus_tag="JNX27_001593"
     CDS             complement(11600..12289)
                     /gene="dgoR"
                     /locus_tag="JNX27_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY7882839.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            12567..13223
                     /locus_tag="JNX27_001594"
     CDS             12567..13223
                     /locus_tag="JNX27_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882840.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13269..14081)
                     /gene="yidA"
                     /locus_tag="JNX27_001595"
     CDS             complement(13269..14081)
                     /gene="yidA"
                     /locus_tag="JNX27_001595"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY7882841.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMGNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14195..14602)
                     /locus_tag="JNX27_001596"
     CDS             complement(14195..14602)
                     /locus_tag="JNX27_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7882842.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(14833..17247)
                     /gene="gyrB"
                     /locus_tag="JNX27_001597"
     CDS             complement(14833..17247)
                     /gene="gyrB"
                     /locus_tag="JNX27_001597"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7882843.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(17276..18349)
                     /gene="recF"
                     /locus_tag="JNX27_001598"
     CDS             complement(17276..18349)
                     /gene="recF"
                     /locus_tag="JNX27_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7882844.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(18349..19449)
                     /gene="dnaN"
                     /locus_tag="JNX27_001599"
     CDS             complement(18349..19449)
                     /gene="dnaN"
                     /locus_tag="JNX27_001599"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7882845.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(19454..20857)
                     /gene="dnaA"
                     /locus_tag="JNX27_001600"
     CDS             complement(19454..20857)
                     /gene="dnaA"
                     /locus_tag="JNX27_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7882846.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            21154..21234
                     /locus_tag="JNX27_001601"
     CDS             21154..21234
                     /locus_tag="JNX27_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882847.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            21464..21604
                     /gene="rpmH"
                     /locus_tag="JNX27_001602"
     CDS             21464..21604
                     /gene="rpmH"
                     /locus_tag="JNX27_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7882848.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            21621..21980
                     /gene="rnpA"
                     /locus_tag="JNX27_001603"
     CDS             21621..21980
                     /gene="rnpA"
                     /locus_tag="JNX27_001603"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7882849.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            21944..22201
                     /gene="yidD"
                     /locus_tag="JNX27_001604"
     CDS             21944..22201
                     /gene="yidD"
                     /locus_tag="JNX27_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7882850.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            22204..23850
                     /gene="yidC"
                     /locus_tag="JNX27_001605"
     CDS             22204..23850
                     /gene="yidC"
                     /locus_tag="JNX27_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7882851.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            23956..25320
                     /gene="mnmE"
                     /locus_tag="JNX27_001606"
     CDS             23956..25320
                     /gene="mnmE"
                     /locus_tag="JNX27_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7882852.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            25562..25636
                     /gene="tnaC"
                     /locus_tag="JNX27_001607"
     CDS             25562..25636
                     /gene="tnaC"
                     /locus_tag="JNX27_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY7882853.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            25857..27272
                     /gene="tnaA"
                     /locus_tag="JNX27_001608"
     CDS             25857..27272
                     /gene="tnaA"
                     /locus_tag="JNX27_001608"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY7882854.1"
                     /translation="MENFKHFPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            27363..28610
                     /gene="tnaB"
                     /locus_tag="JNX27_001609"
     CDS             27363..28610
                     /gene="tnaB"
                     /locus_tag="JNX27_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY7882855.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGARLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            28742..29917
                     /gene="mdtL"
                     /locus_tag="JNX27_001610"
     CDS             28742..29917
                     /gene="mdtL"
                     /locus_tag="JNX27_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY7882856.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            29892..>30537
                     /gene="yidZ"
                     /locus_tag="JNX27_001611"
     CDS             29892..>30537
                     /gene="yidZ"
                     /locus_tag="JNX27_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY7882857.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         7343 a         8088 c         7739 g         7367 t
ORIGIN      
        1 ccggtgcggc agtgaagtac atcgctaaag aaatgcccac ggcaccaaag atttttgagt
       61 taccgtgcag ggcaaaaccg ccttccggga acaacgattt cagcggctcg gcgctcagac
      121 tgaactcttg cagatgctgc gcccagtaca tctgaatgcc gccttcaaca gctgccggac
      181 caaagataaa cggtccgtag atgaagtggt gtaaaccggt tgggatcaga atacgttcga
      241 ggaaggtgta aacccacacc ccaagcgcac ccgccgaacg caggaacgct tgcagagatt
      301 caatccccat ttgtactttt ggccagccga gcaatgtcag ccaggcacag gggatcatca
      361 ccaggaaggc gataatcacc acataagacg tcccctggaa aatgccgaga aaaaccggca
      421 gttttttatc gaacagacgg ttatgcagcg ccgtcacaat gccggaaatg ataattgcgc
      481 cgataatgct ggtatcgagg gttttaatcc cggccatcat tgtcagacca ctacctgcca
      541 ccgcgtcctg agtgaaatcg acgccgaagt agcttcccca ggtcattccc atcgcattga
      601 tgaaatagtt ccaggtcagg aaactcacca tcaccgccag acaagcacgt ccctgcgctt
      661 gcttagcaag gccaatgggt aaaccgacag caaaaatcag cggcatatta cggaataccg
      721 tccaaccgcc ctcttcaata atgtgtacga tttgcgcgaa taaactgttc ggatcggtca
      781 gtgattcccc gacaaacatc gggttttgca gcaagatggc aagacccacc acaatcccgg
      841 cgaagggaaa cagcagcact ggcgtgaaca tcgcgccgcc aaagcgttga atttgactga
      901 gcatttccac atcctctttt ctcaattctg taagtaggaa gcctgattgg ttatgcggca
      961 gacctggagc gaggatagga agatgcgatc ggcagaacat gacgacccaa tgggattcgt
     1021 gatcgctttc atgcttttcg ttgggcggta gagatctacg ttttcgcgaa aaagtagaca
     1081 tctgtgagca caggtacaca gcatcgaaca catctttaaa aaaaagattt ttttcaatcg
     1141 attaagcaga acttgtgggc gcattacccg tgcttgcagg caaaaaagag agctagagat
     1201 gatctacaaa agcattgcgg agcggttaag aattcgactt aattccgcgg atttcacgct
     1261 aaacggcctt cttcccggtg aaaaaaagct ggcggaagag tttgcggtat cgcggatgac
     1321 tatccgtaaa gccattgacc tgctggtagc gtgggggctg gtggtccgcc gccacggcag
     1381 cggcacttac ctggtgcgca aagatgtgct gcatcaaacc gccagcctga ccggactggt
     1441 ggaggtgtta aaacggcagg gaaaaacggt caccagccag gtgctgattt ttgaaatcat
     1501 gcctgcgcct ccggccattg ccagccagtt acggattcaa atcaacgagc agatctactt
     1561 ctcccgtcgc gttcgttttg tggaagggaa accgctaatg ctggaagaca gctatatgcc
     1621 ggtaaaactg ttccgtaacc tttcactgca acatctggaa gggtcgaagt ttgaatatat
     1681 tgaacaagag tgcgggattt tgattggcgg taattatgaa agcctgatgc cggtgctcgc
     1741 cgatagactg ctggcgcggc aaatgaaggt agcggaacac acgccactgc tgcggatcac
     1801 ctcgttgtca tatagcgaga gcggggagtt tttgaattat tcagtgatgt tcagaaatgc
     1861 cagcgaatac taggtggagt accatttacg gcgaccccac ccggaaaaga gttaaccgat
     1921 actccagaag agcaccgcca gtaattgggg ggtgataatg cgcaggaaca tcactaacgg
     1981 atagacagtg gcgtaagaga gcgccgccgc accactggtt ggatgaagat tattagcaaa
     2041 cgccagcgcc ggaggatcgg tcatggaacc tgccagcatc ccgcacatgg tcaggtaatt
     2101 cattttggct aacatccgcg ccagaatgcc aacagtaatc agcggaacgg cggtgatcag
     2161 ggcaccataa ccaatccagc ttagcccttc gccattgacc agggtattca caaaatcccc
     2221 accagatttc agaccaacga ccgagaggaa cagcacgatc cccagctccc gcagcgcgag
     2281 gttggcactt ggcggcataa accagtacag cttgccgata ctgccgatac gcccgaggat
     2341 caacgccata atcagcggac cgcccgccag ccccagtttc aacgcggccg ggaatcctgg
     2401 cacaaagacg ggaatagaac ctaacaatac gccaagcccg atgccaataa acactggcag
     2461 catctgaacc tgttgcagtt tttgctgcgc attccccagc acattggcaa cggcatcaat
     2521 tgcggacgga cgccccacca ggttgaggat atcgccgaac tgcaggctga tatcgccact
     2581 ggcgaccagt tcgaccccgg cacggttcag gcgcgagata acaacgtcat agcgttcttt
     2641 aaagtgcagg tcgcgaatac gttttccgag cacgttttca ttggtgacca ccacacgctc
     2701 gacgcgcaaa tcagtgcctt tcgtagacag cgaggtatcg acctcctgac caatcaccag
     2761 ttgcgcatta tgtaaatccg ctggctgccc caccagatgc agcaaatcgc ccagttggat
     2821 aatggtatct ggcgaaggta cttttagggt ttcttctcgt ttcagacgcg agcagataat
     2881 tttgtcgccg ttgagaatcg gtacatcttt aatggctaaa tcatgcaggt tagggttctc
     2941 aacgcgaata ttgatagtct tgatcagcgc gccgccattg gtgcgtgaag actcgtgctg
     3001 ctgagcttct gtctcgacat tgacgcggaa aataacccgc aacatccaca tggtgaacaa
     3061 aatcccgcaa atgccgaatg gatacgccat cgcgtaactc atccccatct gatcgaccat
     3121 ttccattggt gtacccaggt cgcgcaagat ctgctgccct gcccccagcg ctggcgtatt
     3181 ggttaccgca ccggagaaaa tccccagcac taccggcagt ggaatatcaa acagtttatg
     3241 caggatggcg gtaaccagac caccgatgat gacgatcaga acagcaaaca ggttgaggcg
     3301 taatccggag acgcgcaatg aggcaaagaa gcccggccct acctgaatcc cgatggtata
     3361 aacgaacagg atcaggccaa attcctgaat aacatgcagc atatcgctac ttaatgtcat
     3421 ccccgcctga gaaacaaaat ggccgacgat gatcccacca aacagcacgc cgccaatacc
     3481 taatcctatg ccgcgaaatt tgacgttgcc gataaacaaa ccgacgactg ccaccaaagc
     3541 cagaatactg accgttaatg ctatatcact catcgtccat ttctcttgag ataacatttt
     3601 aattgctacg gattatggct gaatcggtga agcagatttg ccttatagcg cacattatca
     3661 cgggaaagaa cgtgccgaaa tatcttaaac agtcgaaatt taacgcattg ttgaagaaga
     3721 aagacaaaaa aagccccacc agaatggcgg ggcaaagaga atagctagtt agctatttaa
     3781 cgcgggacgt tcgctgatag cgatacgctg cgctgcgatg ggttcaggct cattacgaat
     3841 taaatcaata tgcagtaaac cgtttacgaa ggttgcgcca gagacttcca tattttcagc
     3901 cagcgtaaaa ctcaggctaa atggctgatt cataagccct tgatgcagcc attttttctc
     3961 ttcttttggc tgctccggcg tgccttttac gctcaggcgc gtaccttcca gttgaatctc
     4021 taaatcttcc tgacggaaac ctgccagcgc aagggtaatg cggtagtggt tatcgtcgct
     4081 tttctcaatg ttgtacggcg ggaagctctg gctttcaccg gcgttttgca gtgcgttggc
     4141 cagtttgtca aaaccgatcc attgacgcat cagtggggat aaatcgaagt tacgcatagt
     4201 catttctcct tctaagaagc gagtaagtac ctgcaaatcc gaagattcgc atatgctccc
     4261 tgacggcgag catggagatg tcaggccgcg ccaggcggcc ttagggaatt agttgatttc
     4321 gatacggcgc ggttttttcg cttccggaat cacgcgttcg agatcgatat acagcaaacc
     4381 atttaccagg ttagccccac gaacatgaat gttctcagct aactggaatt tgcgttcaaa
     4441 gttgcgttca gcgatgccct ggtacagata ggtgcgctct ttttgttcgt cggcgtgagc
     4501 acctttcacc accagcagat tatcctgggc ggtaatttcc agttcgctct cagcaaaacc
     4561 agccacagcg atagcaatgc ggtaatggtt ttcgtctacc agttcaacgt tatacggagg
     4621 gtagccgcca ttactctggc tctggttgtt ttctaagtgg ttaaacaaac ggtcaaatcc
     4681 aatagcagaa cggtaaagcg gggataaatc aaagttacgc ataatcaata gctcctgaaa
     4741 tcagcgagaa tgtaagacct tccacaatgg acaggtcagg tagccagaac acccatcagg
     4801 cgcgtcctca tcgactacga tgtaaaatgg gtctggaaat gaacttttca agccttatca
     4861 gcggactttt ttttgcagtt tatggtctat tgcatagact gagggggcag cacgaaacgt
     4921 taaagtgcga tagccgccac agtgcgacgt aatgatgatg ttattttcag caagggatcg
     4981 ctataactca tcaggccaac acccacgatc aacagatgaa tttattatga taagaaatgt
     5041 gttgttagcg ttcatgatat gcagcggaat gacattactc ggaggatgct ccagcgtgat
     5101 gtcccatacc ggggggaaag aaggaacgta tcccggcacc cgcgccagcg ccaccatgat
     5161 tggcgacgat gaaaccaact ggggcactaa gtcgctggcg attctcgata tgccgtttac
     5221 cgctgtgatg gacacgcttt tactgccgtg ggatgtgttc cgcaaagaca gctcggtgcg
     5281 ctcgcgcgtt gaaaaaagcg aagccaacgc gcaagccacc aatgcggtta ttccaccggc
     5341 cagaatgcct gataactgat ttagcgatcg gtttcggtga tccacagctg gccaccttcc
     5401 atccacgcca gccattgacc atccggcgag aagaccacgg cgtccgcaga aggtggattg
     5461 gcgtggtttt cggttaaata ctcaacctcg ccgctttgcg catgggcaca aacaattcga
     5521 ttatccagca caaagcccaa ccattctcct gacggatgcc agttaaatgc agactgaata
     5581 tccgttttgt tatgggttaa cttgcgcggt tcgccgccct gtggcgagat aagccacagt
     5641 tgcacaatgc cgttatcatc acgcattaaa aacgcgattt gcgtaccctg cggattacag
     5701 cgcacccagt ggcgcgggta gggttgtttc cgttccactt aacggcgcat cacctgccga
     5761 tttccagcta gcttcatctt gcggtaactc aacgataaat agctccggca ctttctcgcc
     5821 ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc atccttcttc
     5881 ataagcacga ttgatttcat cactgccagg ctgcggcgtg ggcgtggttt tactcaccag
     5941 cacacaccag tggctaccgc tgtattcacg cggatgttgc ttttgtacgt tgaccgggcc
     6001 aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc ggatcgagtt gatgcattac
     6061 atggtcgtta taggtaaagc tccccctttc accgttcggg ctaaagacat gcacatggct
     6121 gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata tccattgcat cgagattgct
     6181 caccttgccg ccttcagcaa tgactccgcg tcgatgatgg aagtcgtaat gccatgtttc
     6241 atcaggattt tcagggccgt gaataaagac atatttctct gacttcggat gaacggtcac
     6301 cacgccgacg tgtgcgccct gtgacgcgcg atagataacc tcgacctcgc ctgtatggat
     6361 attcacacgc tcaatggtct cgccggtaaa cgacgcccca gaaggacgca cgtcaaatac
     6421 cagccactgg ctgtcgggcg tccaggtatt ggtattggtg agcaggtgat tacggggagc
     6481 aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg gttcccgcca tcatacttca
     6541 caaggacttc actttcagac aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat
     6601 ggaacatttc gacgtggcga ttattggcct cggcccggca gggtcggcgt tggcacgaaa
     6661 gttagccggc aaaatgcagg tgatcgcgct ggataaaaag caccagtgcg gtactgaagg
     6721 tttcagcaaa ccttgcagat tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg
     6781 cgtaactacc agcgaagcta tatcaatatt aatcgccatg ctttcgactt gtggatgaaa
     6841 tcgctgatcc ccgccagcgt tgaggtttat cacgatagcc tgtgccgaaa aatctggcgt
     6901 gaggatgata aatggcatgt catttttcgt gcagacggct gggagcaaca tattaccgcc
     6961 cgctatctgg tcggtgccga tggcgcaaac tcgatggtgc ggcgacatct ctacccggat
     7021 catcaaatcc gtaaatatgt cgctatccag cagtggttcg cagagaaaca tccggtgccg
     7081 ttctactcct gcatctttga taatgcgata actgactgtt actcatggag tatcagcaaa
     7141 gatggttatt ttatctttgg cggtgcctat ccaatgaaag acggtcagac gcgtttcacg
     7201 acgctgaaag agaaaatgag cgcctttcag ttccagtttg gcaaagcggt gaaaagtgag
     7261 aaatgcacgg tgttgtttcc ctcacgctgg caggattttg tctgtggtaa ggacaacgcc
     7321 tttctgattg gtgaagcggc gggatttatc agcgccagct cgctggaggg gattagctat
     7381 gcgctggata gcgcagagat tctgcgtgcg gtgttcctga aacagccgga gaagagcaac
     7441 gccgcttact ggcgcgccac acgcaaactg cgtttaaaac tcttcggcaa gatagtaaaa
     7501 agccgatgcc tgaccgcacc ggctttaaga aagtggatta tgcgcagtgg tgtggcgcat
     7561 attccacagt tgaaagatta tccaacgcgc ttcacatcac ccaccagcag gatgtaagag
     7621 agcgcgccaa tcaacgcgac ggcggagata taaaccagtg caggcgcgaa accgtaaccc
     7681 tgcgccaggt accccaccac cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta
     7741 aacacgccgc cggttaaacc aatcaggcgc atcggtgcca gagaagagac cagcgaccag
     7801 gtaatcgaag caaaaccgtt gccgaagaat gccagcgcca tcaggcacat aatcatcatc
     7861 ggatcgttgg tgtagttagc gcccataatg caggtggaga tcagcaagcc gcagataatc
     7921 ggcgttttac gcgcaaagcc cagtgaaaag cccttacgta ccagcagatc cgcgacccag
     7981 ccagagagca ggacgccgac aaacgccgcg aggaatggca ccgtggtcat aaagcctgct
     8041 ttcagcgccg tgattccttt ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac
     8101 cacagtgcag aagccaccgc aaattgccca agataaacgc cgatcagttt acgatggaac
     8161 accagtttcc agtctttggc tgttaacggc tgacgcgcct ctttcttcac cggcgcatca
     8221 ccatccacca gaccgccgcc atcacgaatg taatccagtt cagctttgct gatacctttg
     8281 gtcaggcgcg gcggctgata aaccttaaac caaatcagcg accagataat gccgatacca
     8341 ccagtgacaa tgaacaccca gtgccagctc aacatctcct gaatccagat cagcagcgga
     8401 gtcaaaaacg ccagaccgac aaactgacca gacgtataaa aaccaacggc ggaagcgcgt
     8461 tcatgttccg gaaaccagct ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg
     8521 aaaataccgg ttatcgcacg cagaccaatt aatgacatta atcccgtggc aaagccctgg
     8581 aataaagtcg ccactgacca accaaatatc gcaataaaat aagtcacgcg agaacctacg
     8641 cgatctaaaa accaaccgcc ggggatctgg catagcgtat aaagccaggc gaaggccgaa
     8701 aatacatagc ccatttccgc tttcgtaatg ccgaactctt cctgaatatg ggcggaagcc
     8761 acggccaggt tagcgcggtc aacataacaa atgaccaccg taataaagat catcaccagc
     8821 gtcagataac gccgacgccc cggctttgct gcattaacgg gaatatccat agcgagcttt
     8881 ctccagattt tgggcatagc gaagccgctc accatgccct gtaatttaca gagggttata
     8941 tttttgtatt gctgttttag tgcccgatga ggggcttacg tggcaggaat taccactctg
     9001 ctacgctgtt atcttcatga cgccagagcg gattacgcca gtccggggca tttttactga
     9061 actcaataac tttagcttcg tcgatttcca cacctaagcc cggtttcgtt aacggtttaa
     9121 agaagccgcc aaccatgctg aagtcttctt tgtttttcac aaagtcgagt aactccgcgc
     9181 ctttgttgta atgaatgccc atactttgtt actgaagtac cgcgttatag gaaacaaagt
     9241 cgatatgcag gcaagccgcc agtgcaatcg gtccgaacgg acagtgcggc gcaagggtca
     9301 catcataggc ttctgccatt ccagcaattt tgtagcattc ggtaataccg cctgcatggg
     9361 agagatccgg ttgcagaatc gaaataccgc ctgcctccag cacgcgttta aaatcgaaac
     9421 gtgagaacat gcgctcaccc gccgccagtg gaatatgcgt ttgtgccgcc aatttcgggt
     9481 agtattcggc ttgttccgcc agcaccggct cttcaataaa cagcgggcga tacggctcca
     9541 gttctttaat cagcactttc gccattggcg cgctgacgcg accgtggaaa tcaagaccaa
     9601 actcaatctg attgccaaaa gcttcacgaa tttgtgccac ggtgttgact gccgcatcta
     9661 ccgcgcggga gttatcaatt agccccagtt cttcacaacc gttcagtttg aaggtatcga
     9721 agccgatttc gcgcagggtt ttaatgccgt cgataacatc cgccggacga tcgccgccga
     9781 cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc catcagttgc cagactggcg
     9841 cattcagcac tttgcctttg atatcccata acgcctggtc gatcccggcg atggcgctca
     9901 tcaggattgg accgccacga taaaatccgg cgcgatacat cacttgccat aagtcgttga
     9961 tgcgcgaagg atcctgacca atcaaatagt cacccagctc gtgaactgcc gcttccaccg
    10021 tacgggcgcg gccttcaatc acgggctcgc cccaaccgac cacgccttca tcggtttcaa
    10081 ttttcaggaa catccagcgg ggaggtaaac gatacgtggt aattttggtg attttcattg
    10141 cactgcctct cgatacgcct taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga
    10201 ctgcccggcg cgataaagat cgctgcctaa gcccgcccca acacaacctg cgtctatcca
    10261 ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag actgcgatgt ccggtggtaa
    10321 taccgctttt aacgctttga tgtattgcgg accaaaagcc gatgacggaa atattttcag
    10381 cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca
    10441 aacggtcatg ccgtagccca ccgcgcggcg gatcacttca ctatggatat tgggcgtaac
    10501 gatgagctga cagcccatcc tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc
    10561 tgcgccaatc aacgccttgt cgccatacgc atcaacgatg gcgggaatgc tctgctccca
    10621 ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac
    10681 atgcgccagc gcctcgtcgg gcgtaatgcc gcgcaaaatg gcgatcagcg ggagtttagt
    10741 ttgccactgc atgagcgatg ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg
    10801 ccgccacgtc gcaacccatc gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc
    10861 cggcgacaag ggtgatggcg tgttgatggg tcacatagtc gcgcatactg gcaacctctg
    10921 cgccaatcaa caaaccagag agaatgttcg cggagaagtg ttcccagcac atgcgaggcg
    10981 cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc aaggccagcc
    11041 gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa tgagtgattt
    11101 aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat ttgctggcta
    11161 tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga aggagccaga
    11221 gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt atcgtcatga
    11281 gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa ttgttcgcca
    11341 atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc gacattgctg
    11401 ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt cgtgacttct
    11461 gctaacaccg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg
    11521 ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga tccccagtca
    11581 attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag ctggcaatca
    11641 tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca tcgaacagcg
    11701 ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag gttcgttcaa
    11761 aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc gggttatgca
    11821 ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct
    11881 gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg ctggaggtcg
    11941 cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact tcgctcatgg
    12001 cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg tcagtgtcga
    12061 ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc atttcaatca
    12121 gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag gttgcaaact
    12181 cttcacagag ctccgcctca gccggaagtg gcgagccagg tacgtatttg ccgtgaacga
    12241 tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg gagagtcctt
    12301 gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc aactttgtag
    12361 taccagcgtg atgacgttcg cgtttgccgt gcgtgtaatg tagtacaaac ttatattgtt
    12421 gtgctacaat ttagatcaaa aaaagaacaa tgcataaaaa aatgacatgc gtcgggcaga
    12481 aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc tttcaacggc
    12541 ttagctactc gtttaaaaga ttaatcatga agttgaattt taagggattt tttaaggctg
    12601 ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct ctggtttccc
    12661 ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc actgggctat
    12721 cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt
    12781 accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca gcacttaacc
    12841 ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat
    12901 ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag gcactggcaa
    12961 cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa aacctaaaag
    13021 gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg ttctaccatc
    13081 catttaaagt gcgattttat gaatactatt cacccagagg cattccggat ggtgtttccg
    13141 cagcattact gccagtgact gttacgctgg acatcattac tgcaccgctg caatttctgg
    13201 ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg
    13261 cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc ttcaaggttg
    13321 gattttgtga caaagttcgc catttctttc accgaaggaa tagcgttacc catcgccaca
    13381 ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt
    13441 tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc tttgttaacg
    13501 cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata tttctctttc
    13561 acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc aatcatcatc
    13621 actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaatt
    13681 gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc ggtgtacagc
    13741 gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa
    13801 cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc ggccttctgt
    13861 accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc
    13921 aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt cacgccacgg
    13981 gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga
    14041 agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca aggtagatta
    14101 gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag
    14161 gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc cctttcagca
    14221 gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca ccttgcggtg
    14281 agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc gtactggtat
    14341 ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc gattccagtt
    14401 gctcgccgct aactgattga tttctctgtt gattactcaa ccaagttgag agaatggccc
    14461 ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc tcctcaaccc
    14521 aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacttcat
    14581 caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatc
    14641 tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct ccgccctttt
    14701 cttatagctt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgcaaa
    14761 atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg cacgctcgca
    14821 tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt caataaacgc
    14881 acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag cggcaatcgc
    14941 atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg tttcccacag
    15001 ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc cacgacggga
    15061 ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac gacgctcgcc
    15121 acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat
    15181 acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag tatccacacc
    15241 gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg tgtggacatc
    15301 aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac
    15361 ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg gctgatagat
    15421 aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca ttttctgcgt
    15481 cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg gtgcactggc
    15541 gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat ccatcgcttc
    15601 gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg gcggctgagc
    15661 gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga aggtcaacag
    15721 cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata
    15781 acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg cggtgataag
    15841 cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga cgttaaggat
    15901 tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc
    15961 gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc gttcctggca
    16021 gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc gggtcatttc
    16081 acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc caaccacgat
    16141 tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca tctgctgttc
    16201 aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg agaatttcgg
    16261 gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc
    16321 gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca gggtacgggt
    16381 catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt
    16441 aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt caacgccaat
    16501 accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt tgttcttgtt
    16561 cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc
    16621 acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg
    16681 tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga ggctgggcca
    16741 gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg gagcctgcgg
    16801 tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa ccagctccag
    16861 tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt
    16921 ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc
    16981 cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat cctgtacaga
    17041 gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc
    17101 gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt
    17161 gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat
    17221 actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat
    17281 ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa
    17341 cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg
    17401 caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac
    17461 accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag gcgcacatca
    17521 acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc
    17581 gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat
    17641 tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg aaagagaaag
    17701 tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc gcgataccgg
    17761 cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt tctttatccc
    17821 acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc tgcttgagca
    17881 atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag catccccagt
    17941 cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta
    18001 tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc gtaccgtcaa
    18061 tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc tcgcgctctt
    18121 cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg acgcgaccaa
    18181 tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc agcacgctgg
    18241 ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa gcgagatccg
    18301 cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat
    18361 tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca cgctggaaac
    18421 cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa catccagcac
    18481 atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc
    18541 ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt cgctgacata
    18601 caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa acgcctgctt
    18661 gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa cgcgacggta
    18721 atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa cgtgggcacg
    18781 aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga gcatacgcat
    18841 cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag attgaccaat
    18901 tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca gttcttcacc
    18961 ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg ccatcgaaaa
    19021 ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct
    19081 ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg aaaaacggct
    19141 acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt ccgcgccttc
    19201 aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg ctcctggctc
    19261 gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag taccggtcag
    19321 cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg
    19381 accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt
    19441 aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct tctttgatat
    19501 cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt
    19561 cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc tctttcgcta
    19621 gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat
    19681 ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca
    19741 gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg
    19801 cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc agctcacgta
    19861 cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa cgaatgtcat
    19921 tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc ggttcgatcg
    19981 ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt
    20041 tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg
    20101 tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca
    20161 gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg
    20221 ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct
    20281 tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc agacccgtgc
    20341 cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc tggcgagccg
    20401 ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt
    20461 ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa cctgagcgcg
    20521 tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc
    20581 tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct
    20641 gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt
    20701 cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggacagcgtg ttatcgctca
    20761 gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca
    20821 atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag
    20881 tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgct
    20941 tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg
    21001 atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt
    21061 ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct gccgcgaggc
    21121 gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat
    21181 cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt ataatcctcc
    21241 acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc tgcgaaaagg
    21301 tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca attattacaa
    21361 tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc
    21421 caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca
    21481 accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa
    21541 tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa
    21601 gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa
    21661 ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta
    21721 ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga
    21781 aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc
    21841 gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg
    21901 ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc
    21961 gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct
    22021 actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg
    22081 caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc
    22141 tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta
    22201 acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc
    22261 tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca
    22321 acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa
    22381 ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt
    22441 gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg
    22501 ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc
    22561 ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg
    22621 ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac
    22681 acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac
    22741 gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt gaagcaatcc
    22801 atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgt
    22861 ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga taccattgcc
    22921 gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct gcaacagtat
    22981 ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac cgctaatctg
    23041 ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca gcctggtcag
    23101 actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa aatggcagct
    23161 gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat ctctcagccg
    23221 ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc
    23281 atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc
    23341 atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat
    23401 gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg
    23461 ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg
    23521 ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg
    23581 gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag
    23641 aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac ctttatgccg
    23701 gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta ctatatcgtc
    23761 agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc
    23821 ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg
    23881 tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc cgccacacaa
    23941 agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac acctccggga
    24001 cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt tgccgaaacc
    24061 gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa agacgccgac
    24121 ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc
    24181 gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct gctgttaaaa
    24241 cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg
    24301 tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct tatcgacgcc
    24361 agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg
    24421 gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat
    24481 ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca gcttaatgac
    24541 gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa
    24601 gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct gttaaacgcg
    24661 ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg tgacgtgctg
    24721 cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc cgggctacgt
    24781 gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga aattgaacag
    24841 gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga tccggcagag
    24901 atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt ggtacgcaat
    24961 aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca cgcgttaatt
    25021 cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct caaacagagc
    25081 atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca cttacaggcg
    25141 ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg agcctgggca
    25201 ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga aatcaccggg
    25261 gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat tggtaagtaa
    25321 ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt gttagcactc
    25381 ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat aatctttaaa
    25441 aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta aacaatttca
    25501 gaatagacaa aaattctgag tgtaataatg tagcggcgtg tctttcgagg ataagtgcat
    25561 tatgaatatc ttacatgtat gtgtgacctc aaaatggttc aatatcgaca ataagattgt
    25621 cgatcaccgc ccttaattta cccttctgta gccatcacca gagccaaacc gattagattc
    25681 aatgtgatcg attagtttgc tatatcttaa ttttgccttt tgcaaaggcc atctctcgtt
    25741 tatttactta ttttagtaaa tgatggtgct tgtatatata catggtgaat taatggatat
    25801 tgcagatgta atattcacag ggatcactgt aattaaaata aatgaaggat tatgtaatgg
    25861 aaaactttaa acatttccct gaaccgttcc gcattcgtgt tattgagcca gtaaaacgta
    25921 ctactcgcgc ttatcgtgaa gaagcaatta ttaaatccgg tatgaacccg ttcctgctgg
    25981 atagcgaaga tgtgtttatc gatttactga ccgacagcgg caccggggcg gtaacccaaa
    26041 gtatgcaggc agcgatgatg cgcggcgacg aagcctacag cggcagccgc agctactatg
    26101 cgttagccga gtcagtgaaa aatatctttg gttatcaata tactattccg actcaccagg
    26161 gccgtggcgc agagcaaatc tatattccgg tactgattaa aaaacgcgag caggaaaaag
    26221 gcctggatcg cagcaaaatg gtggcattct ctaactattt ctttgatacc acgcagggcc
    26281 atagccagat taacggctgt accgtgcgta acgtctatat caaagaagcc ttcgatacgg
    26341 gcgtgcgtta cgactttaaa ggcaactttg acctcgaagg attagaacgc ggtattgaag
    26401 aagttggccc gaataacgtg ccgtatatcg ttgcaaccat caccagtaac tctgcaggtg
    26461 gtcagccggt ttcactggca aacttaaaag cgatgtacag catcgcgaag aaatacgata
    26521 ttccggtggt aatggactcc gcacgctttg ctgaaaacgc ctatttcatc aagcagcgtg
    26581 aagcagaata caaagactgg accatcgagc agatcacccg cgaaacctac aaatatgccg
    26641 atatgctggc gatgtccgcc aagaaagatg cgatggtgcc gatgggcggc ttgctgtgca
    26701 tgaaagacga cagcttcttt gatgtgtaca ccgagtgcag aaccctttgc gtggtgcagg
    26761 aaggcttccc gacatatggc ggcctggaag gcggcgcgat ggagcgtctg gcggtaggtc
    26821 tgtatgacgg catgaatctc gactggctgg cttatcgtat cgcgcaggta cagtatctgg
    26881 tcgatggtct ggaagagatt ggcgttgtct gccagcaggc gggcggtcac gcggcattcg
    26941 ttgatgccgg taaactgctg ccgcatatcc cggcagacca gttcccggca caggcgctgg
    27001 cgtgcgagct gtataaagtc gccggtatcc gtgcggtaga aattggctct ttcctgttag
    27061 gccgcgatcc gaaaaccggt aaacaactgc catgcccggc tgaactgctg cgtttaacca
    27121 ttccgcgcgc aacatatact caaacacata tggacttcat tattgaagcc tttaaacatg
    27181 taaaagagaa cgcggcgaat attaaaggat taacctttac ctacgaacca aaagtattgc
    27241 gtcacttcac cgcaaaactg aaagaagttt aattaatact acagagtggc tataaggatg
    27301 ttagccactc tcttacccta catcctcaat aacaaaaata gccttcctct aaaggtggca
    27361 tcatgactga tcaagctgaa aaaaagcact ctgcattttg gggtgttatg gttatagcag
    27421 gtacagtaat tggtggaggt atgtttgctt tacctgttga tcttgccggt gcctggtttt
    27481 tctggagtgc ctttatcctt atcattgcct ggttttcaat gcttcattcc gggttattgt
    27541 tattagaagc aaatttaaat tatcctgtcg gctccagttt taacaccatc accaaagatt
    27601 taataggtaa cacctggaac attatcagcg gtattaccgt tgccttcgtt ctctatatcc
    27661 tcacttatgc ctatatctct gctaatggtg cgatcattag tgaaacgata tcaatgaatt
    27721 tgggctatca cgctaatcca cgtattgtcg ggatctgcac agccattttc gttgccagcg
    27781 tattgtggat aagttcgtta gccgccagtc gcattacctc attgttcctc gggctgaaga
    27841 ttatctcctt tgtgatcgtg tttggttctt tcttcttcca ggtcgattac tccatcctgc
    27901 gcgatgccac cagcaccact gcgggaacgt cttacttccc gtatatcttt atggctttgc
    27961 cggtgtgtct ggcgtcattt ggtttccacg gcaatattcc cagcctgatt atttgctatg
    28021 gcaaacgcaa agataagtta atcaaaagcg tggtatttgg ttcgctgctg gcgctggtga
    28081 tttatctctt ctggctctat tgcacgatgg ggaatattcc gcgcgaaagc tttaaggcga
    28141 taatctcctc aggcggcaac gttgattcgc tggtgaaatc gttcctcggc accaaacagc
    28201 acggcattat cgagttttgc ctgctggtgt tctctaactt agctgttgcc agttcgttct
    28261 ttggtgtcac gctggggttg ttcgattatc tggcggacct gtttaagatt gataactccc
    28321 acggcgggcg tttcaaaacc gtgctgttaa ccttcctgcc acctgcgttg ttgtatctga
    28381 tcttcccgaa cggctttatt tacgggatcg gcggtgccag gctgtgcgcc accatctggg
    28441 cggtcattat tcccgcagtg cttgcaatca aagctcgcaa gaagtttccc aatcagatgt
    28501 tcacggtctg gggcggcaat cttattccgg cgattgtcat tctctttggt ataaccgtga
    28561 ttttgtgctg gttcggcaac gtctttaacg tgttacctaa atttggctaa atccttcaag
    28621 aagccagcca ttcgctggct tcttgcctct caggaaatca cttatgtcca aatggcaact
    28681 cgccttatcc tccttcacca cgtatgcttt gcgtcacctt actatcagga cgctttagcc
    28741 catgtcccgc tttttgattt gtagttttgc cctggtttta ctttatcccg ccgggattga
    28801 tatgtacctc gttggtttac cgcgcatcgc cgccgatctc aatgccagcg aagcgcagtt
    28861 gcatattgcg ttctccgtat atctggcggg gatggcagct gcgatgctat ttgccggtaa
    28921 agtggccgat cgttcaggga gaaagccggt cgccataccc ggcgcggcgc tatttattat
    28981 tgcctcggtg ttctgttcac tggctgaaac cagcacgtta tttcttgcag gccgatttct
    29041 acaggggttg ggcgcaggct gttgttacgt ggtggcgttc gccattttgc gcgacacgct
    29101 ggatgatcga cgtcgggcta aagtgctgtc attactcaac ggtattacct gcatcattcc
    29161 ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa ttcccgtggc agagtctgtt
    29221 ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg tctttgttta ttttaaaaga
    29281 aacgcgccca gcgtcccccg ctgcttcgga caaaccacga gaaaatagcg agtcgctgct
    29341 taatcgtttt ttcctcagcc gtgttgttat caccaccctc agcgtttcgg tgatcctcac
    29401 tttcgtcaat acatcgccgg tattgctgat ggaaatcatg gggtttgagc gcggagaata
    29461 cgccaccatt atggcgctga ccgctggcgt cagcatgacc ttttcattct cgacgccatt
    29521 tgcgctggga atttttaagc cacgtacgtt gatgatcacc tcgcaggtgt tattccttgc
    29581 ggcggggatc acccttgccg tttcaccttc ccatgcggtt tctctgtttg gtatcacgct
    29641 gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg agtcaggcgt taggaccatt
    29701 ttcattacgc gcgggcgtag ccagctcgac cttaggtatt gcgcaggttt gcggttcgtc
    29761 actgtggatt tggctggccg cggtggttgg tatcggcgca tggaatatgc tgatcgggat
    29821 tctgattgcc tgtagcattg tgagcctgtt gctgattatg ttcgtcgcgc ctggacgccc
    29881 cgttgccgct catgaagaaa tccatcacca cgcttgatct caatctgctg ctctgtctgc
    29941 aactgctgat gcaggagcgc agcgtgacca aagcggcgaa gcggatgaac gtgacccctt
    30001 cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt tgacgacccg ctctttgtga
    30061 actcaccgct ggggctgtcg cccacacagc taatggtcag catggagcaa aatctggcgg
    30121 aatggatgca aatgagcaat ctgctgctgg ataaaccgca ccaccagaca ccgcgcggcc
    30181 tgaagtttga gttggcggcg gaatcgccgc tgatgatgat catgcttaat gcgctgtcga
    30241 aacggatcta ccaacgttac ccgcaggcga ccatcaaact acgtaactgg gattacgatt
    30301 ccttagatgc cattactcgt ggtgaagtgg atattggttt ttccggtcgc gaaagccatc
    30361 ctcgctcgcg ggagctgtta agctcgctac cgttagccat tgattatgaa gtgctgttta
    30421 gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc actgcatgaa acgtggaatc
    30481 tggacacctt tttacgttat ccgcatatca gcatttgctg ggaacagagc gacacct
//
LOCUS       DADLXX010000035        30527 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLXX010000035 DADLXX010000000
VERSION     DADLXX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.035"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..588
                     /locus_tag="JNX27_001612"
     CDS             <1..588
                     /locus_tag="JNX27_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7882858.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="JNX27_001613"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="JNX27_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7882859.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="JNX27_001614"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="JNX27_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7882860.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="JNX27_001615"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="JNX27_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7882861.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="JNX27_001616"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="JNX27_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7882862.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="JNX27_001617"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="JNX27_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7882863.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="JNX27_001618"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="JNX27_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7882864.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="JNX27_001619"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="JNX27_001619"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7882865.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="JNX27_001620"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="JNX27_001620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7882866.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="JNX27_001621"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="JNX27_001621"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7882867.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15250)
                     /gene="thiG"
                     /locus_tag="JNX27_001622"
     CDS             complement(14405..15250)
                     /gene="thiG"
                     /locus_tag="JNX27_001622"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7882868.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIVDKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="JNX27_001623"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="JNX27_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7882869.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="JNX27_001624"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="JNX27_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7882870.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="JNX27_001625"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="JNX27_001625"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7882871.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="JNX27_001626"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="JNX27_001626"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7882872.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="JNX27_001627"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="JNX27_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7882873.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="JNX27_001628"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="JNX27_001628"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7882874.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="JNX27_001629"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="JNX27_001629"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7882875.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="JNX27_001630"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="JNX27_001630"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7882876.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="JNX27_001631"
     CDS             22192..22782
                     /locus_tag="JNX27_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7882877.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="JNX27_001632"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="JNX27_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7882878.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="JNX27_001633"
     CDS             23254..23949
                     /locus_tag="JNX27_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7882879.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="JNX27_001634"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="JNX27_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7882880.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="JNX27_001635"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="JNX27_001635"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7882881.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="JNX27_001636"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="JNX27_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY7882882.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="JNX27_001637"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="JNX27_001637"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7882883.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="JNX27_001638"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="JNX27_001638"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7882884.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7147 a         7951 c         8403 g         7026 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga tgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttctg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag gaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgaaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaactaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagaaccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagttgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cacaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatgag cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctaggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgcct
//
LOCUS       DADLXX010000036        29733 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLXX010000036 DADLXX010000000
VERSION     DADLXX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.036"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            347..640
                     /locus_tag="JNX27_001639"
     CDS             347..640
                     /locus_tag="JNX27_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882885.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JNX27_001640"
     CDS             712..1371
                     /locus_tag="JNX27_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7882886.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JNX27_001641"
     CDS             1448..1909
                     /locus_tag="JNX27_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7882887.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNX27_001642"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNX27_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JNX27_001643"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JNX27_001643"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7882888.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /locus_tag="JNX27_001644"
     CDS             3808..5076
                     /locus_tag="JNX27_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7882889.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSTSVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNX27_001645"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNX27_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7882890.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNX27_001646"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNX27_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7882891.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="JNX27_001647"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="JNX27_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7882892.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="JNX27_001648"
     CDS             complement(8331..9863)
                     /locus_tag="JNX27_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7882893.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARHYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /locus_tag="JNX27_001649"
     CDS             10193..11491
                     /locus_tag="JNX27_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7882894.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..12571
                     /gene="dadX"
                     /locus_tag="JNX27_001650"
     CDS             11501..12571
                     /gene="dadX"
                     /locus_tag="JNX27_001650"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7882895.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12739..14165)
                     /locus_tag="JNX27_001651"
                     /pseudo
     CDS             complement(12739..14165)
                     /locus_tag="JNX27_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14775..16511)
                     /locus_tag="JNX27_001652"
     CDS             complement(14775..16511)
                     /locus_tag="JNX27_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7882896.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNX27_001653"
     CDS             complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNX27_001653"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7882897.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17620..18231
                     /gene="emtA"
                     /locus_tag="JNX27_001654"
     CDS             17620..18231
                     /gene="emtA"
                     /locus_tag="JNX27_001654"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7882898.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLRYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18233..18967)
                     /locus_tag="JNX27_001655"
     CDS             complement(18233..18967)
                     /locus_tag="JNX27_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7882899.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19168..19422
                     /locus_tag="JNX27_001656"
     CDS             19168..19422
                     /locus_tag="JNX27_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7882900.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19472..21425)
                     /locus_tag="JNX27_001657"
                     /pseudo
     CDS             complement(19472..21425)
                     /locus_tag="JNX27_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(21451..22305)
                     /locus_tag="JNX27_001658"
     CDS             complement(21451..22305)
                     /locus_tag="JNX27_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY7882901.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22305..23114)
                     /locus_tag="JNX27_001659"
                     /pseudo
     CDS             complement(22305..23114)
                     /locus_tag="JNX27_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(23124..23882)
                     /locus_tag="JNX27_001660"
     CDS             complement(23124..23882)
                     /locus_tag="JNX27_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7882902.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APIIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23879..24859)
                     /locus_tag="JNX27_001661"
     CDS             complement(23879..24859)
                     /locus_tag="JNX27_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7882903.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24859..25881)
                     /locus_tag="JNX27_001662"
     CDS             complement(24859..25881)
                     /locus_tag="JNX27_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY7882904.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWLPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26947..27387
                     /locus_tag="JNX27_001663"
     CDS             26947..27387
                     /locus_tag="JNX27_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882905.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(27466..29163)
                     /locus_tag="JNX27_001664"
     CDS             complement(27466..29163)
                     /locus_tag="JNX27_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY7882906.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLGTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(29483..>29733)
                     /locus_tag="JNX27_001665"
     CDS             complement(29483..>29733)
                     /locus_tag="JNX27_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7882907.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         7456 a         7226 c         7638 g         7413 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcac gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaatg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca gcaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgtt
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacccctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgtttttg
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa acccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agtggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgcccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttaatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg cgtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcgctc ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttgggcttcc agcccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg
    12061 ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg
    12121 gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac
    12181 cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc
    12241 gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat
    12301 tgtcgccgca ggatacgccg acggttatcc gcgccatgcg cctaccggta ctcctgtttt
    12361 agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga
    12421 tttaacgcct tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat
    12481 caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct
    12541 ggcgttacgc gtcccggttg tgacagtgta acttattgta agccggatcg gaggcagcgt
    12601 cttctggttg cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
    12661 agagccattg cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga
    12721 actcgccgca caggcgttct acataaaacg cttacgcttc attattgact cggagtcgac
    12781 ttagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tacagttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag gcgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag ttccaccgac aggaagagcc gccctggcgg gcgtcgtacg
    14101 tctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta
    14161 gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag acgtgaatgt
    14221 ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc atcgttgact
    14281 ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag
    14341 ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt tattaagcaa
    14401 aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga aacgcctatt
    14461 gtgtgggcta tgtcagtgcg gcaggttgcg tggggaatat gttcaggcga tgtcaggcag
    14521 gggcccctga tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa
    14581 aaagccctta cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa
    14641 tggtttgtca tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg
    14701 aaatgacaag tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag
    14761 atgctgcatt aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac
    14821 acttcattat cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca
    14881 ccgacaaccg gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta
    14941 tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg
    15001 ctggcttcga gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg
    15061 ctgaacagtt taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg
    15121 ccttcgcgca gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct
    15181 gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta
    15241 tcagcactca attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca
    15301 acgcgtgaca ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac
    15361 gagagtgatg ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag
    15421 aacagtcgcg cattctccag ccccgccatc atcgggaaca cggccaggat gattggcacc
    15481 gcgccgcgta atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa
    15541 gggagcaatc cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa
    15601 atgagcgccg gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc
    15661 aggaacatgg cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag
    15721 cggttgcgaa tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg
    15781 ctgccttcca gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat
    15841 ggatataatc cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg
    15901 ccaagaccga tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa
    15961 ctgacactgc tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc
    16021 attggatcat tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc
    16081 ttaccaccca gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt
    16141 aataagcctt caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca
    16201 gaggtgataa gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag
    16261 gaactggcct gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt
    16321 accatgtagg cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc
    16381 agcatgccga tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa
    16441 ctaagtaata tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt
    16501 gtggcatcca acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat
    16561 tagcggcaga gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac
    16621 aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg ttattcagaa tggcatgtgc
    16681 gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg
    16741 aatcgacagg cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata
    16801 atcattgggc gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat
    16861 tcccgcatga tagagctgca acagcatacg ttcgacgcgg aaagggtgct cgttaatatc
    16921 ttccagcacc agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat
    16981 cagcatcgca agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc
    17041 ttgccattca atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa
    17101 ggcgttcagt tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac
    17161 attgccttgc gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag
    17221 taacgggtca tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg
    17281 actggcaccg taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc
    17341 aagggaattg agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg
    17401 ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg
    17461 cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat
    17521 ggcaattcct tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg
    17581 tcacctcaac ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc
    17641 tttttgattg tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg
    17701 aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt
    17761 gcagcctggg gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt
    17821 aatccgaacg cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc
    17881 tccggacgtg atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg
    17941 aaaaatccag agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc
    18001 ccgctggcag gcattgaaga tccgaaggta ctgcgatatg cgctggtggt gtcatacgct
    18061 aacggggcag gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa
    18121 atcaacgatt tggatgctga tgagttcctc gatcacgtag cgcgaaatca tcctgcgccg
    18181 caggctccgc gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc
    18241 gcactttgtc cgctttttcc cgtgcttctc gctcgagaga gaaaataatc cgctgtaatt
    18301 gccgctccac cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg
    18361 tttcattctt gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt
    18421 gaaaaacacc ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta
    18481 attcggcagg ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca
    18541 agcggaaacg taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga
    18601 gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta
    18661 cggtaataaa tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact
    18721 cgactttcgc accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga
    18781 tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta
    18841 ttttgctgat cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca
    18901 aatcgcgtaa cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat
    18961 gactcacgcg gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac
    19021 aaaacttaat acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac
    19081 gcccagcatc gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga
    19141 ccgattcatc gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg
    19201 atagccggca ttatcgccaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg
    19261 acctgtattc tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc
    19321 attggcggct ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct
    19381 atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat
    19441 agcgatacag atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga
    19501 aattgcgacc ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat
    19561 tgaccgacgc attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca
    19621 ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct
    19681 cttccgacag cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg
    19741 tctgcgttgg aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca
    19801 gagcgtagct caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca
    19861 gaccgctgta atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt
    19921 gcgagaggat ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc
    19981 gcgtgaaggc accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgtg
    20041 ggttaacaat cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg
    20101 tcggaaagcg tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt
    20161 atttccccat cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc
    20221 tgccatcttt ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg
    20281 ccacgacatc gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac
    20341 gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt
    20401 tccagttaac ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc
    20461 cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag
    20521 ccagcgagtt gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca
    20581 gggtaaaacg gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact
    20641 ctggccactg ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt
    20701 tttcaccatc ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac
    20761 cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc
    20821 ctgcaatatc attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac
    20881 cgctgacttg caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg
    20941 cattgtcctg actacggctc catccctggc gatatcccag acttgcttct tcagggtggt
    21001 gatattaatg gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga
    21061 aacttcgact gcccccagat tgttggtcag aacccgcgcc agatcgaggt tgccgtcata
    21121 gggaacataa atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg
    21181 aacttttacc tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac
    21241 acttaatgcc tgagcgacat tctgtttatc caaggctttc atggtgggtt gatcgagaac
    21301 ggtggtcgtc gtcgatgata ccggactgga ccatacggtc agcgtgtcgc ctcctgagcc
    21361 gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt
    21421 ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgtgggtt gcagactgac
    21481 tttaatgtca gctggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca
    21541 gtcgaggtaa tttttgagtg tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg
    21601 aggagccagc gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc
    21661 gagctgaagt acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc
    21721 ttcaacaaca attttcttct cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca
    21781 gccctgattg tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca
    21841 tccggcacga tgaattagtc ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt
    21901 gcccggaatt gcttttcggg tgcaggcgat attgccgtca ggataacgtt cacgaagcaa
    21961 tgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac
    22021 tgccttccat ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgttgacc
    22081 cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa
    22141 catcttacac gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaacgctat
    22201 atagccaggc tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc cctggatatc
    22261 ttccagcagt aacgcatcga tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc
    22321 ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca
    22381 tcttgttgtt gataccagtg atataaccgc tcacgctcat cgtcgttaag cgcgccaaga
    22441 ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt
    22501 ccacggataa aatcaacgtg ggcataaatt cccatctgat acaggacgtt gagcgcaaag
    22561 atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg
    22621 aacgaggtgc tgaccagatg agttgtatag acgcgaagcc gtgcctgatt attcagtttg
    22681 ctcattgcat ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag
    22741 cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtcagcc
    22801 ttcagtgcgg cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca
    22861 taaaccgtag tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc gcagcccata
    22921 tcgaataagg tctgagcgcc ttgcaggtca attttagcga tcagttgttg caggtagctg
    22981 tccgttggcg aggctcaatt ttccgccatt ttctccgccc gttgatccca gtgatcgggc
    23041 gttttttctg tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc aaaatcaata
    23101 tcatcaatga gcatgattgt tccttagcgg ctaattgcag agaggtgatg atgaatttgg
    23161 tcggcagaaa cccgatacag ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag
    23221 ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgttggca
    23281 tggagcggat ggtgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc
    23341 gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcgggctc atccagtaac
    23401 aacaagcgcg gttgttggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg
    23461 ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc
    23521 gtggctttca ggcgttcttg cttaccggga acggaaaaag cgcctatgat gggggccagg
    23581 cccatcaaca ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc
    23641 caggcaatag cctgtgcgcg ctggcgatgg ctgagtgtct gcacagggag gctatccagt
    23701 aggatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg
    23761 ccgcaaccat tcgcgccaag cagacaacaa attgttcctt gcgacagggt gaaggacacg
    23821 tcgcgtagca ctggatggcg ggagtaaccc agagacgcat gacgaacggc aagcaaagtc
    23881 actggcgacc tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca
    23941 gaatgccaag tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga
    24001 gcagaatagc gccagtacac attgccatag gcaacagctg ctgatggtta tgaccggtca
    24061 acaagcgggc aatgtgcgga acgaccaggc cgatccaacc aattatgccc gctattgcta
    24121 ccgtgctggc ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt
    24181 tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga
    24241 gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta
    24301 aggtcacggt ggacagtccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag
    24361 gatcggccag cgttttaatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc
    24421 ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga
    24481 cgcccgcgac caccatcagc tcgccgcaaa atgcgtacag ctggatgtaa acaatgggta
    24541 atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat
    24601 ccggtgaaac taagggatta cgcaacattc cctgataggt ggttccggct cctgccagcg
    24661 ctgcgcccag taacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt
    24721 gttcctgcac agacacattt tcttgacgaa gaattagcgc cagcagttgc tgcatatcga
    24781 gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac
    24841 atccgtttac gattctcatt agctcgccac caatttttga tagtcggcat cactgagtga
    24901 acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc
    24961 gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc
    25021 atcgagccag ccaaagggca agccacttaa aaataagatg cgttgttccg caacggcttt
    25081 cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag
    25141 aataatatcc ggcagccagc gcaggagatt ttccatggaa acctgtgtca ggccgtggcg
    25201 atcggctatt tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga
    25261 accctgtaag cccgtttcca gccctcgggg accgcgcgca gcataaaagc ggaggttagc
    25321 ggcgggtgag gtggcgaatg cctgagcttc accaacgaag tggctggcga gattggcttg
    25381 ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga
    25441 ttgcgccagt tttccgttaa gcaataacca ggggatttgt gtctgttcgc taacctgccg
    25501 tgcctgggag atcaaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa
    25561 cgccataagc ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg
    25621 taactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga
    25681 aaaaccgacc attttttctg gcgcgacagc cagcaatagc aaatcggcgg gcgcgcctgc
    25741 gctgacgatg cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa aacgattaac
    25801 tgtttgtgaa aaggacggta gagactgcag gagcagggta gatgcaagtg cctgcgcaaa
    25861 cgtccgtctg gttatgggca tggtgaacat cctgaaaagc aaaatcgata tatactttca
    25921 tatatatcga taaaaatcat gttcaatatc aaagtttagc tattgaaaac atgaagatgt
    25981 gacgacttcc gcaagcctgt tgaccctctc tatcattaaa aatgtattta tcaggtggct
    26041 aatattattt aaatcattta aggctaaatc atttaaatat gaatgacatt attgtttttt
    26101 atcgtccacg gttacgcgac gttgaagtta cttgttttac aatgagcgat ttttgatatc
    26161 tcgccgagaa aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag
    26221 gtgaaaagaa tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt
    26281 gctgatagta agcagcgtaa ccagaagtta ccgcaacaag actttgagga ttttaagaac
    26341 gacgttatta atgtcattaa caacaatgaa gatatttatt ttcttagaga gagtaattta
    26401 ctgaatgcgt ttattcgaac gtgcaaaaaa agagatgata acagttacgg aaatatgata
    26461 tttacgcttt attttgataa cgatatctat gggaacagaa ttatcaaagc atcgctgaac
    26521 ggcggagagt tgcagttatt aagcacaggg tgtagcaatg ataacattct ccattttatt
    26581 aatgcatttc tcattcgggt gaaatttccg ctacctggac gcataaaacc ggaactctcg
    26641 ccaaaagccg ggcactccga aggaattgac gcgttgcgct gctctgtaac ggcatttacc
    26701 gcaagccatt tatttcgcca gcgacaatcc gcagagaacc aaacgcatca ggaaacgact
    26761 gaacacaata aatactgtat tgatcaacat attatactgg cacctccagc acgcagtgag
    26821 gatacactgt atcatcagcg aatcttatca ctgcggagct ggttgaacga ctttctgagc
    26881 gaaataagtc ggttcccggt aacaacaaca gaatcctttt ttaattattg tttcgttgtt
    26941 tttggtgtga ttcagaaaat aaatatttta gacagtagcc agtcaggcgt taaaaatggg
    27001 gcggatagtg aggcggtttt ggctgcgctt attgaacatt tagaattgat caatccttct
    27061 ggcagattaa gccaaaatac ccgttcggca atgcttcagt tacgcgaaga ggaatggtct
    27121 gaattctttt tttggttgct aaattctctg gagtgtctgg attatgtgat tattaattta
    27181 actccagaaa gcaaaaaaac gttgatgagt gaacaccgta acaatattca ggtagcaatt
    27241 gatgctttat atagccaacg tcggcgtaaa tcgccgggtg atgaaagtga aacacttacc
    27301 cgtagaaatg acgctatctt cggcaaccac gtatggcaaa catttgcgca atacttcccg
    27361 ccaggacttg aaaaaccgtc tgtgtagaaa aaaccataga tatggtgtta tctgccgaca
    27421 acgccatccg gagaagccga atgacgttgt cggagtaagc gctggttaag gtgtgggttg
    27481 tgcctctttg gttgagggtt gcgtcgttgc tgacttaacg gtcggacgcg tcgccggaac
    27541 attgtcacac ggttgctctt tcgggcagat caaatccagc attttcagcg tcacgccatt
    27601 ggtccagcca aagccatcct gtaatggata ttcgccaccg ccgccccccg ttccggtggt
    27661 gctgacatca tatttttcca ccagcttttt ctcccggtca taggtgtgct gaacattggt
    27721 caggaagtgc cagctaatgt ccatcgccac ctctttttgc ccgtagtttt gtaatccttc
    27781 tgtcgcgacc cactgtaacg gtgcccagcc atttggcgca tcccattgtt gcccactttt
    27841 caccgacgtg gtgttcaggc cgccgggttg cagcagatgt gttttcgtcg ccgtcgccat
    27901 tttgttggcg cgatctttcg ctgccgcatt gacgtacagc gggaacaggg cggccgcggt
    27961 taactgattg cgcactttat gacttttcag gtcgtaatcg gcataccagc cttgttgatc
    28021 gttccacagg tatttttcga tccctttttg acgggcattc gccagcgttt cgtactggtt
    28081 tgccatcgcg ttatctcctg cagctttgct ggcgcgggcg aggatttttt ccattttaaa
    28141 catcaggctg ttcagatcga ccggtacgat gctggtggtg cctaaggtat ttaactgctg
    28201 cgggttgtcc atccagcgcg agctgaaatc ccagccagac gcagcggcag agcgcaggtc
    28261 gcggtaaatt tcagtggcag gtcgattcgg attgcttttg gcggtggcaa tatcttccac
    28321 ccatgactct ggtcgtggcg tatcgcgatc gtcccagtag cggttgagaa gggtaccatc
    28381 ctgaagtttt acaacgcgtt tttcctgttg tccggcttgc aggttttcaa caccgtccat
    28441 ccagtaagca tattcttttt gcatttgcgg caggtattgc ttcaacgcgg catcgccttc
    28501 atgctgcgcc agtaactcta ccatcagggc aaagaagggc ggttgcgagc ggcttaaata
    28561 gtaactgcgg ttgccgttgg gaatatgacc gtaagtgtct atttcatgag caaaattggc
    28621 caccatatcc gcgactttat cccagtgacc gctttcggca agtcctaaca tggtgaagta
    28681 actgtcccag taatatacct cgcgaaaacg tccgcccggc acgacataag gttccggcag
    28741 cggtaacaga gaatcccatt tttcggtgtt ttcggtagaa cgcgttaata ccggccaaag
    28801 tccgtcaata tgttcgcgca gtgactgccc ctctggcgga acatatttct cgccttcttt
    28861 cggcagggtg aaattgacgt taacgaaatg gcgcagatca aatccgctct ggttttgctg
    28921 catccgataa tcagcaagga tcatcagcgg atcgctgttc ggcacggcat cggcaaaggt
    28981 tttttggtcc ggaaaaagtt tggcgttttg cacatcatta aacagcggcc ctaataaaat
    29041 atcaggcggc tgtggtgtta ccggtgtttc ttctgcctgc accgatagcg cagcgaaaca
    29101 caaaaagata caggctggaa ttaacgccat tttttgcggg cgagaaggtg cgggggattt
    29161 catcaatcat tctcctttgg cgaaaccgaa taaaacggta ttcaagtatc agaaaacctt
    29221 agttcaggat cgcactagct gcatgatcga aatctcattc tgcgaacggt caggcatttt
    29281 agaaaaggga attaaaggga taaataacgg cgaaatagag tgatcaaata acgtctgttt
    29341 attagccagc cagcgacatt tggttcgcga ttagcgaaga aaaatcatca taaattggta
    29401 aaaaatataa caggtagtta aaaaacatca gtgctgggta aattgccgga tgacatcaga
    29461 acgatgccat ccgaacggtg gcttaaccct gacggttgaa acgttgcgtt ttaacgtcca
    29521 gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg caaatccagc
    29581 caatccccag ttcacgggcg attagggcgc tgtgggatac cggacttccg gcgctaaggc
    29641 agataccttt tacaaccgcc ggatccagtt gcagtactgt ggaaggataa atgttctccg
    29701 ccagtagaat agttggcgag ttaaactgcg gga
//
LOCUS       DADLXX010000037        28640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLXX010000037 DADLXX010000000
VERSION     DADLXX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.037"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            16..4593
                     /locus_tag="JNX27_001666"
     CDS             16..4593
                     /locus_tag="JNX27_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7882908.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4961..6616
                     /gene="lldP"
                     /locus_tag="JNX27_001667"
     CDS             4961..6616
                     /gene="lldP"
                     /locus_tag="JNX27_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7882909.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6616..7392
                     /gene="lldR"
                     /locus_tag="JNX27_001668"
     CDS             6616..7392
                     /gene="lldR"
                     /locus_tag="JNX27_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7882910.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7389..8579
                     /gene="lldD"
                     /locus_tag="JNX27_001669"
     CDS             7389..8579
                     /gene="lldD"
                     /locus_tag="JNX27_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7882911.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8775..9248
                     /gene="trmL"
                     /locus_tag="JNX27_001670"
     CDS             8775..9248
                     /gene="trmL"
                     /locus_tag="JNX27_001670"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7882912.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9301..10122)
                     /gene="cysE"
                     /locus_tag="JNX27_001671"
     CDS             complement(9301..10122)
                     /gene="cysE"
                     /locus_tag="JNX27_001671"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7882913.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10202..11221)
                     /gene="gpsA"
                     /locus_tag="JNX27_001672"
     CDS             complement(10202..11221)
                     /gene="gpsA"
                     /locus_tag="JNX27_001672"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7882914.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11221..11688)
                     /gene="secB"
                     /locus_tag="JNX27_001673"
     CDS             complement(11221..11688)
                     /gene="secB"
                     /locus_tag="JNX27_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7882915.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11751..12002)
                     /gene="grxC"
                     /locus_tag="JNX27_001674"
     CDS             complement(11751..12002)
                     /gene="grxC"
                     /locus_tag="JNX27_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7882916.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12144..12575)
                     /locus_tag="JNX27_001675"
     CDS             complement(12144..12575)
                     /locus_tag="JNX27_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7882917.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12820..14364
                     /gene="gpmM"
                     /locus_tag="JNX27_001676"
                     /gene_synonym="pgmI"
     CDS             12820..14364
                     /gene="gpmM"
                     /locus_tag="JNX27_001676"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7882918.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14374..15657
                     /gene="envC"
                     /locus_tag="JNX27_001677"
     CDS             14374..15657
                     /gene="envC"
                     /locus_tag="JNX27_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7882919.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15661..16620
                     /locus_tag="JNX27_001678"
     CDS             15661..16620
                     /locus_tag="JNX27_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7882920.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16607..17641)
                     /gene="waaH"
                     /locus_tag="JNX27_001679"
     CDS             complement(16607..17641)
                     /gene="waaH"
                     /locus_tag="JNX27_001679"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY7882921.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17880..18905)
                     /gene="tdh"
                     /locus_tag="JNX27_001680"
     CDS             complement(17880..18905)
                     /gene="tdh"
                     /locus_tag="JNX27_001680"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7882922.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18915..20111)
                     /gene="kbl"
                     /locus_tag="JNX27_001681"
     CDS             complement(18915..20111)
                     /gene="kbl"
                     /locus_tag="JNX27_001681"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7882923.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20386..21243)
                     /gene="yibB"
                     /locus_tag="JNX27_001682"
     CDS             complement(20386..21243)
                     /gene="yibB"
                     /locus_tag="JNX27_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY7882924.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21548..22480
                     /gene="rfaD"
                     /locus_tag="JNX27_001683"
     CDS             21548..22480
                     /gene="rfaD"
                     /locus_tag="JNX27_001683"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7882925.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22490..23536
                     /gene="rfaF"
                     /locus_tag="JNX27_001684"
     CDS             22490..23536
                     /gene="rfaF"
                     /locus_tag="JNX27_001684"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7882926.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23540..24532
                     /gene="rfaC"
                     /locus_tag="JNX27_001685"
     CDS             23540..24532
                     /gene="rfaC"
                     /locus_tag="JNX27_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7882927.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLDYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24529..25737
                     /gene="rfaL"
                     /locus_tag="JNX27_001686"
     CDS             24529..25737
                     /gene="rfaL"
                     /locus_tag="JNX27_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY7882928.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25773..26915)
                     /locus_tag="JNX27_001687"
     CDS             complement(25773..26915)
                     /locus_tag="JNX27_001687"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY7882929.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26924..27937)
                     /locus_tag="JNX27_001688"
     CDS             complement(26924..27937)
                     /locus_tag="JNX27_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY7882930.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27962..>28640)
                     /gene="rfaY"
                     /locus_tag="JNX27_001689"
     CDS             complement(27962..>28640)
                     /gene="rfaY"
                     /locus_tag="JNX27_001689"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7882931.1"
                     /translation="KVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTAR
                     GPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTL
                     NFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSE
                     KGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKV
                     KLGLKSK"
BASE COUNT         7461 a         7088 c         6922 g         7169 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cggctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaataaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     1921 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     1981 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2041 ggcggcaatg cttctgtgaa tactgacggc gtgtttaccg gtccaaccta caaaatcggt
     2101 gaaacaaatt attataacgt cggcgatgca ctggctgcga ttaactcctc atttagcacg
     2161 tctctcggcg atgctctgct ttgggatgcc accgcaggta aattcagtgc caaacacggt
     2221 actaatggtg acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc agactccagt
     2281 tctgacgcag taaacggctc acaactccac ggcgtgagca gttatgttgt tgatgcgctg
     2341 gggggtggtg ccgaagtcaa tgcagacggc accatcactg cgccgacgta caccattgct
     2401 aatgctgatt acgataatgt cggtgatgcc ctgaatgcta tcgataccac tcctgacgac
     2461 gctctgctct gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa
     2521 gataaaactg ccagtgtaat cactaacgtc gctaacggtg caatctctgc tgccagcagc
     2581 gacgcgatta acggctcaca actctatacc accaataagt acatcgctga tgcgctgggt
     2641 ggtgacgcag aagtcaacgc tgacggcacc atcaccgcac cgacttacac cattgcgaac
     2701 gccgagtaca acaacgtcgg tgacgccctg gatgcgcttg atgataacgc cctgctgtgg
     2761 gataagactg ccaatggcgg tgctggagcc tacaatgcca gccatgacgg taaagccagc
     2821 atcatcacta atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc agtgaacggt
     2881 tctcagttga atgcgacgaa tatgatgatt gagcagaaca cccaaattat caatcagctc
     2941 gctggtaaca ccgacgcaac ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt
     3001 actaacgaca acgatttagc ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct
     3061 gtaggttata acgctgtcgc gtctggtgcc agcagcgtag ccattggtca gaacagcagc
     3121 agcaccgttg ataccggtat tgcgctgggt agcagctccg tttccagccg tgtgattgcc
     3181 aaaggttctc gtgacactag cgtaacggaa aatggcttgg tgattggtta tgacaccact
     3241 gacggcgaac tgctaggtgc attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc
     3301 aacgtagctg atggttcaga agcccatgac gccgttacgg ttcgccagtt gcagaacgct
     3361 attggagcgg tcgccactac gccaaccaag tactttcacg ccaactcaac ggcagaagac
     3421 tcactggccg ttggtgaaga ctcgctggca atgggtgcga aaactgtcgt taatggtaat
     3481 gcaggtattg gtatcggttt gaacactctg gttctggctg atgcgatcaa cggcattgct
     3541 atcggcagta acgcacgcgc aaaccatgca aacagtatcg caatgggtaa cggttctcag
     3601 accacccgtg gtgcacagac tggctacacc gcctacaaca tggacgcacc gcagaactct
     3661 gtaggtgagt tttctgtcgg tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct
     3721 ggttcagcgg ataccgatgc ggttaacgtg ggtcagttga aagtcactga tgagcgcgta
     3781 gcgcaaaata cccagagcat tactaacctg aacaatcagg tcactaatct ggatactcgc
     3841 gttactaata tcgaaaacgg tattggcgac attgtcacca ccggtagcac caagtacttc
     3901 aagaccaaca ccgatggcgt agatgccaac gcccagggta aagatagcgt tgctattggt
     3961 tctggttcca ttgctgccgc tgacaacagc gtcgcactgg gtaccggttc cgttgcagag
     4021 gaagaaaata caatctctgt aggttcttct actaaccaac gccgtatcac caacgtagct
     4081 gccagtgtta atgccaccga tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc
     4141 ggagtgcgct acgacaccaa agctgatggt tctatcgact atagcaatat caccctcggt
     4201 ggtggcaacg gcagtacgac tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac
     4261 gcggtgaact acgcgcagtt gaagcaaagc gcgcaggaaa cgaagcaata caccgatcag
     4321 cggatggttg agatggataa caaactgtct aaaactgaaa gcaagttgag cggtggtatc
     4381 gcttctgcaa tggcaatgac cggtctgccg caggcttata caccgggtgc cagcatggct
     4441 tctattggtg gcggtactta caacggtgaa tcggcagttg ctttaggtgt atcgatggtg
     4501 agcgccaatg gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac
     4561 tccgccgcac tcggtgccgg tattcagtgg taatgatcca ttaagttagt gtgactaagg
     4621 gcaaacgcca aattgcctga tgcgttacgc gcatcaggca agataaggcg ctccgggtca
     4681 acaatctaag ggcaattctc tgatgaggat tgcccttttc tttaccagac atctcccccc
     4741 acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa ttccccaatt
     4801 aaaactcaca tcaatattgc taatacataa catttagtta accattcatt gtcattatcc
     4861 ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca
     4921 gactcattac acgatgtgcg tggactctag gagacctgca atgaatctct ggcaacaaaa
     4981 ctacgatccc gccgggaata tctggctttc cagtctgata gcatcgcttc ccatcctgtt
     5041 tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt
     5101 agcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc
     5161 ctcggtggtt tatggcttct tctatggttt gtggcccatc gcgtggatca ttattgcagc
     5221 agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc gctcgtctat
     5281 tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct gtttcggcgc
     5341 tttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg cattgctggt
     5401 cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc
     5461 agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa caggcatcga
     5521 cagctttgag attggccaga tggtggggcg tcagctaccg tttatgacca ttatcgtgct
     5581 gttctggatc atggcgatta tggacggctg gcgcggtatc aaagagacgt ggcctgcggt
     5641 cgtggttgca ggcggctcgt ttgccatcgc tcagtacctt agctctaact tcattgggcc
     5701 ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa
     5761 acgctggcaa ccagttcgcg tattccgctt cggtgacttg ggggcgtcac aggttgatat
     5821 aacgctggcc cacaccggtt acaccgcggg tcaggtgtta cgtgcctgga caccgttcct
     5881 gttcctgacc gctaccgtaa cactgtggag catcccgccg tttaaagccc tgttcgcatc
     5941 gggtggtgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctca ataaactggt
     6001 tgcccgtatg ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt ttaagtttga
     6061 ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga ttgtctggct
     6121 gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct
     6181 acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact attccggact
     6241 gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct tcttctcgcc
     6301 gttcctcggc tggctggggg tattcctgac cgggtcggat acctcatcta acgccctgtt
     6361 cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt tgctggttgc
     6421 cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc
     6481 ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca
     6541 cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac
     6601 gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt
     6661 gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc
     6721 caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct ggcaaaactg
     6781 gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat
     6841 gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc cgatgatccg
     6901 gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac cgcatggcat
     6961 gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt tgaagcaacg
     7021 ctaagtgaag accctgatat cgcctcacaa gcggacgttc gttttcatct ggcgattgcc
     7081 gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa
     7141 tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt ttcacaactg
     7201 accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga cggggcgcgt
     7261 aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt cgatgaagat
     7321 caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca ttcgagggag
     7381 aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc
     7441 cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg ctgcgccgca
     7501 acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac atgtccgact
     7561 taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca ctggcaccgg
     7621 tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg
     7681 cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc
     7741 cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc ggctttatgc
     7801 gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc accgtggata
     7861 tgccgacacc gggcgcacgc taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg
     7921 caatgcgccg ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga
     7981 acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg accggactgg
     8041 aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg
     8101 agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc gatccggaag
     8161 atgcgcgcga tgcagtacgt tttggtgctg atggaattgt ggtttctaac cacggtggcc
     8221 gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca gatgcggtga
     8281 aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta
     8341 tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa
     8401 cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgaaaaagag atgaaagtgg
     8461 cgatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg ctggtgcagg
     8521 ggctgggtaa agagttgcct acggcactgg ctccgatggc gaaagggaat gcggcatagt
     8581 cgtttgcgcc ccctcaccct aaccctctcc ctcagggaga ggggaccgtt cggcgctgta
     8641 tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc cctccgggga
     8701 gggccggggt gaggggaaaa ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc
     8761 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
     8821 gcaacatcat ccgtctttgc gctaataccg gctttcgtct acatatcatc gaaccgatgg
     8881 gatttgcctg ggacgacaag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
     8941 ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt
     9001 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag gatggcgatt
     9061 atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg
     9121 ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaat ctgtccaatg
     9181 cggtgtcggt agtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga
     9241 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca
     9301 ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc
     9361 cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt
     9421 tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc
     9481 aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
     9541 cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
     9601 tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat
     9661 aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg
     9721 gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg
     9781 ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac
     9841 tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg
     9901 gtcagcggcg taggcttctt caaccacttc acggatagca atagcaggca taattggcga
     9961 tgacagcttg ttcgccagca tgtagctcag tgcactgcca aggttttcgt gcttgagtag
    10021 cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc
    10081 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
    10141 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg
    10201 gttagtggct gctgcgctcg tccttgcgtg cacgacctaa taatgtcaat gctgcctcgc
    10261 gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc atttcaacgc
    10321 cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac
    10381 caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg ccaaaacggc
    10441 ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc gcccagcccc gccatgccca
    10501 taaaggtggc aggatcggca cccagcgccg caccaagacg cgacatttca gccagcccac
    10561 gggtgatcag cgccgtacgc gcattcgcac caaaaccgat accgtcagac atccccgcgc
    10621 caatggcaat gacgtttttc accgcgccgc caagctgcac gccaatgaaa tccgggttgc
    10681 tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct
    10741 gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg
    10801 ttgggccaga gataaccgcc agcggaattt gatcgcctaa ggcctcacgc gccacgtcct
    10861 gtaacagacg tccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag
    10921 gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga
    10981 cgagaatatt acgactggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat
    11041 cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga
    11101 tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtaatgg
    11161 caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca
    11221 tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa
    11281 cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc gggataccat
    11341 gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca
    11401 atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc
    11461 acacaggaac gcggtttctt cgcccaagga ggccgttacg gtaacacgca gtaccacttc
    11521 gtatacgtca tctgccagtt gggaagatgc cgtatccaga tcaagtttaa cttctggttg
    11581 ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat ccttggtata
    11641 aatacgttgg atctggaaag tcatttcagt gttgttttgt tctgacatgt gtagaaaacc
    11701 cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa cgttcacacg ttatttcagc
    11761 aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc
    11821 tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca
    11881 cgcttggcgg cattgccatc gatcggcaac tcctggaaac tcacgccctt gctgctcagc
    11941 agtgcttttg cacgatggca atacgggcag gtttctttgg tatagatttc aacattggcc
    12001 ataatttagc tcctgttttt ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg
    12061 agtgtcccca gaagcttata acaataagtc gctgggggcc taattgcagc taacggcctg
    12121 catcatgaaa gacgacaggt aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc
    12181 cagccagcaa cgccctcttt cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg
    12241 tttgcaggct cctggcactg catgccagaa ccgtctacca cgataaccga tttgtctttg
    12301 tgcttctcaa gctcaccaac attgttggct ttgatttcgc tcggcaacag attgatagaa
    12361 cctgcgatat ggcctttacg gaagtcatca cgctgacgta aatccacaac cacagcgtct
    12421 tctttgttga tcagacgcgt agcttcacca cgagtaatca ccttcacttt cgaggtcagg
    12481 ctcttaaacg tagtcacaag aaccgccacc agtaacgcga tccaggcgat actcagtatg
    12541 ggatgacggc caacaaattg cataatttct tgcatggggg gtaacaactc ccaacgtagt
    12601 gattaaaaaa accaggaaag gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt
    12661 aactggaatg caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg
    12721 ccgcgagcgg tcgtatattt tgatcttcag aggctatttt atcgattcag ctgtagtaaa
    12781 attacgcaaa ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct
    12841 atggtactgg tgattctgga tggctatggc tatcgtgaag aacagcagga taacgccatt
    12901 tttagtgcta aaaccccggt aatggatgca ctgtgggcca atcgtccgca taccctgatc
    12961 gacgcttccg gtctggaagt cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc
    13021 cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa
    13081 atcaaagatc gcgctttctt tgctaatcca gtgctggctg gcgcggtaca taaagcgaaa
    13141 aacgcaggta aagcagtaca cattatgggt ctgctctccg caggcggcgt acacagccac
    13201 gaagatcaca tcatggcgat ggtagaactg gcagctgaac gcggcgcaga aaaaatctac
    13261 ctgcacgcat tccttgacgg tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa
    13321 aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt
    13381 tactacgcga tggaccgcga taaccgttgg gatcgcgttg aaaaagccta tgacctgctg
    13441 actctggcgc agggtgagtt ccaggccgat actgccgttg ctggtttgca ggcagcttat
    13501 gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca
    13561 gatgcggcaa tggaagacgg cgatgcgctg attttcatga acttccgtgc tgaccgcgcg
    13621 cgtgaaatca ctcgtgcatt cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg
    13681 gttaacgtcg atttcgtgat gctgaccgaa tacgccgctg acatcaaaac tgcggttgct
    13741 tacccacccg cttccctggt taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact
    13801 cagttgcgta tttccgaaac cgaaaaatat gcccacgtta ctttcttctt caacggtggc
    13861 gtagaagagt cgttcaaagg cgaagatcgc attctgatca actcaccgaa agtggctacc
    13921 tacgatctgc aaccggaaat gagctccgca gagctgaccg aaaaactggt tgcggccatc
    13981 aagagcggca aatacgacac cattatctgt aactatccga acggcgacat ggtaggtcac
    14041 accggggtga tggaagcggc ggttaaagcg gttgaagcac tggatcactg cgtggaagaa
    14101 gtcgcgaaag cggttgagtc cgtgggtgga caactgctga tcaccgctga ccacggtaac
    14161 gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca cggcacacac caacctgcca
    14221 gttccgctga tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg caaactttct
    14281 gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt
    14341 aagccgctgt tcatcgtgga ataatccctc cccatgaggg gaaaggcgat taataccatg
    14401 acacgggccg tgaaaccgcg caggtttgca atcaggccca tcatctacgc cagcgttctt
    14461 agcgctggcg tattgttgtg cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa
    14521 tctattcagg ccgatatcgc cgcaaaagag cacgcggtac gccaaaagca acaacaacgc
    14581 gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc cacccgtaag
    14641 ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac agattgatga gatgaacgcg
    14701 tcgattgcca aactggagca gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg
    14761 gatgccgcat tccgtcaggg cgagcatacc ggtattcagc taattctcag cggtgaagaa
    14821 agccagcgtg gacagcgttt acaggcttat ttcggctatc tcaaccaggc gcgacaagaa
    14881 accattgccc agttgaagca aacgcgtgaa gaagtcacca tgcagcgtgc tgaactggaa
    14941 gagaaacaaa gcgagcaaca aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg
    15001 actcaggttc tgaacgagcg taaaaagacg ctggcggggc tggaatcttc catccagcaa
    15061 ggtcagcagc agctaagtga actgcgtgcc aacgaatccc gtctgcgtaa cagcattgcc
    15121 cgtgcggaag ccgcggcgaa agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt
    15181 cgcgagcgcc agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa
    15241 aaatcgctga tgtcccgaac tggtggcctg ggtgcgccgc gtggtcaggc attctggccg
    15301 gttcgcgggc cgacgctgca tcgctatggc gagcagctac agggtgaatt acgctggaaa
    15361 gggatggtta tcggtgcctc tgaaggtact gaagttaaag caattgccga cggtcgagtg
    15421 attctggctg actggctgca aggttacggt ctggtggtgg tggttgagca cggtaaaggc
    15481 gacatgagtc tttacggcta taatcagagc gcactggtga gcgttggttc gcaggttcgc
    15541 gcgggccagc caattgcact ggtgggcagc agtggcggtc agggtcggcc ttcactctat
    15601 ttcgaaattc gccgccaggg tcaggcggtc aatccacagc cgtggttggg aagataagtt
    15661 ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct ctcctccccc
    15721 gtacttgctg gcaaacttgc catcgtcatt gatgattttg ggtatcgccc gcacaacgaa
    15781 aaccaagtgc tggcgatgcc ttccgctatc tccgtcgctg tattacccga ttcaccgcac
    15841 gccagagaga tggcgaccaa agcgcataac agcggtcacg aagtgttgat tcatctcccg
    15901 atggcaccgt tgagtaaaca gccgctggag aaaaatacgc tacgcccgga gatgagcagc
    15961 gacgaaattg agcgcattat tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc
    16021 aacaaccaca tgggcagcaa gatgacctct aacctgtttg gtatgcagaa agtgatgcag
    16081 gcgctggagc gttacaatct ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg
    16141 atgcgcgccg cgcaaggtac tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat
    16201 tcgcagaacg aagcggacat ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc
    16261 aacggttcaa ccattgccat tgggcatcct cacccttcaa cggtacgcgt gctacaacag
    16321 atggtttata acctgccgcc agacattacg ctggtgaaag ccagcagctt gctgaatgaa
    16381 ccgcaggttg atacttctac accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg
    16441 ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag aacctgttta tgctaatcgc
    16501 ttctttgaag tattaagcga aagcatcagc cagagcacgc tgatcgttta cttccagcat
    16561 cagtggcaag gctggggcaa acagcacgaa gcggcgaagt ttaacgctag cgcaaattaa
    16621 aggcgctacg cgtaatgcga tggtgcgacc gcgttttgtc gcgccattgc cataagcgga
    16681 aagaccacag taacgcctgg taaccgacct tcacgctgcg cacattggta atcaggcgct
    16741 tatacatacc ggaagtaaat atctcggcaa tcatccgttg gcgggtaaga atatccggct
    16801 ctttgcgcac cgcatggcaa acacgcaatg cttcgtaagt tatttgctga tgaaattccg
    16861 gataaatcgt aattttgtcg gcataatttc gatttaattt ctccagcagg cgggtaatct
    16921 taatatagtg acgttgataa ttaaggtttt tattcccttg tctatgcaac cgactcactg
    16981 acgtattatg cagataatat ttatataatg attgctcggt atatcgcgca cgcagcgcgt
    17041 taaacatgaa ttctgttgtc cagacaatat cctgatgatg taatccggca ataaatttaa
    17101 tgttattttt aacaataaca tcacggcgat aaacccccat ccagacaacg tgagtccaac
    17161 ggcgcgaaga aagccccatc cgcagccagt ccgggccggt taatacaccg gttgagcgaa
    17221 ggcgatcgct ggggatggat tgccaggttt ctcccgtttc acgaaaagac cagtcagcgt
    17281 tgcactgcgc cacgtcgagg tcgtcctcta acgccatggt catcagcgtt tcgtacatgg
    17341 tgggatagac ctcatcgtca gcatcgacaa aagcgacata tttgcccgtc gccacctcga
    17401 tcccacgatt acgcgctacc gatgcgccag cattcgcctg atgcaacaaa cgaacgtgcg
    17461 gatagttttc tgcgtaatgc tttgctattt caacagaatt atccgttgaa ccatcgttaa
    17521 taataatgat ttccagagca gtccaggttt gcgtaattaa agattccata caagtgcgga
    17581 aatcatcgcc cgcattatat aacggaataa taacactaag tttattggtg ctgttcatca
    17641 taaattaccg acgacctctg aagttatcct gacgttttac aggctttgta ttaagaaact
    17701 attaagcctg tgagatatta tttttacagg tgagagagtc acttcagggt agagagataa
    17761 tgagaacagc tatataaaag atcagcgcaa ttacgaactc tttattacac tgacaggatt
    17821 ttacataatt aacagattat cctcagataa aagggctgga ataccagccc ttgttcgtgt
    17881 taatcccagc tcagaataac tttcccggac tggcccgaac gcatagcgtc aaagcccttc
    17941 tggaaatcat cgatagagaa acgatgggta atgatcggcg agagatcgag gccagactga
    18001 atcagcgccg ccatcttgta ccaagtttca aacatctcac gaccgtaaat gcctttaatg
    18061 aacaagcctt taaagatcac tttggtccag tcgatagaca tatcagacgg tggaataccc
    18121 agcatcgcaa tacgaccgcc gtggttcatg gtatcgagca tggtacgaaa cgctggcggc
    18181 gcgccggaca tttccagacc gacatcaaag ccttcggtca tgcccagttc agccatcaca
    18241 tcattaaggt tttctttcgc aacgttaacc gcacgggtga tacccatttt gcgcgccagc
    18301 tcaaggcggt attcgttaac atcggtgatc accacattgc gtgcaccaac gtgtttcgcc
    18361 accgccgcag ccataatacc tatcggacct gcaccagaaa ccagcacatc ttcgcccacc
    18421 agatcgaacg acagcgcggt atgcacggcg ttaccgaagg gatcaaaaat ggaagccagg
    18481 tcgtcggaga tattgtcggg gattttgaaa gcgttgaacg ccggaatcac cagatattcg
    18541 gcgaagcagc ccggacggtt aacaccgaca ccaatggtgt tgcggcacag atgggtacga
    18601 ccgccacgac agttgcgaca gtgaccgcag gtgatatggc cttcgccaga aacgcgatcg
    18661 ccgattttga agcctttcac ttcctgacca atacctacca cttcaccgac atattcatgg
    18721 cccacgacca tcggcaccgg gatggttttt tgcgaccact catcccagtt atagatgtga
    18781 acgtcagtcc cgcagatggc tgttttacgg attttaatca gcagatcgtt atgcccgagt
    18841 tccggtacag ggacgtcggt catccagatg ccctcttccg ctttcagttt ggataacgct
    18901 ttcatctcac atcctcaggc gataacgccc agttgtttac caatacgcgt gaatgcttct
    18961 actgcacgcg taatttgctc aggggtatgc gccgcagaca tctgggtacg aatacgcgcc
    19021 tgacctttcg gaacgaccgg atagaagaaa ccggtgacgt aaatgccctc tttttgcagc
    19081 tctcgggcaa atttctgcgc cactaccgca tcaccaagca tgaccggaat aatggcgtga
    19141 tcggctcccg ccagggtaaa gcccgccgcc gacatttgct cacggaactg acgcgcgttc
    19201 gcccacagac ggtcacgcaa ctcgctaccc gcttcaacca tctccagcac tttgatggaa
    19261 gcggcgacaa tcgctggtgc cagcgagttg gagaacaggt acggacgaga acgctggcgc
    19321 agccactcaa ccacttcttt gcgcgccgcg gtataaccac cagaagcccc gcccagcgct
    19381 ttaccgagcg tgccggtgat gatgtcgaca cgccccatca catcgcagta ttcatgggaa
    19441 ccacgaccat tttcaccgac aaaaccgacc gcgtgggagt cgtctaccat caccagggca
    19501 tcgtatttat ctgccagatc gcaaacgccc ttcagattgg caatcacgcc gtccatcgag
    19561 aacacgccat cggtggcaat cagcacatga cgcgcaccgg cttcacgcgc ttctttcaga
    19621 cgagcttcaa gctcctgcat atcgttgttg gcatagcgat agcgtttagc tttacacaga
    19681 cgcacaccat caataataga cgcgtggttc agtgcgtcgg agataatggc gtcttccgcg
    19741 cccagcagcg tttcaaacag gccaccgtta gcatcaaagc aggaagagta gagaatcgca
    19801 tcttccatcc ccaggaatgc cgccagtttt tgttcaagct ctttatggct gtcctgagtg
    19861 ccgcaaataa aacgcaccga agccatgccg aaaccgtgag aatccattcc cgcctttgct
    19921 gccgcaatca gatccggatg attcgccagc ccgagatagt tgttggcaca aaagttaatg
    19981 acgtggcttc catcagccac ggtgatatct gcttgctgcg cagacgtaat aatgcgctct
    20041 tctttaaaca acccttccgc ccgtgcggtt tccagatcgt tggttaactg ctgataaaat
    20101 tctccacgca ttgcgattct ccagacaggg caaattccag cacatattac ccaaacttat
    20161 aggtcgcgac gagatgatgc gttaacactt ctgcaaaatt caggataaat aacgtgtatc
    20221 ttgatgtctc tcaacaccta cagatagtag aaaaactgta cagttttggc tgagcataat
    20281 gatgagtgat tttactgcat tgatataaaa agaaaaaatg atgccaaagc atcatttttc
    20341 ctatctcagt tggcatcaaa acgctaccca ctaatggagg gaaacctatt ttcttgaaag
    20401 gaaaattctc agcgcctgca tttttgcccc taaagtgtta ctcctgaagc agcgaaacaa
    20461 atcgaaccat tttcctctcc ccagatagtt taggttgaag agatcggaac gtttgtaata
    20521 gcacatgaca aaaatgccct ggtcatcgtc cacaatgtta ttgtttagtg ttattttttg
    20581 actttccaga actaatttat agaattcttt ccatttatgc tgtgaaccaa caattgcgcc
    20641 cccaataata tatacatgat tgccaatcat gaaatcaaaa gcctgttgtt gagatgtaac
    20701 agtaagccct ttttttatag tgaaaagatg catcttattt tcatcaaatg ggaaatccca
    20761 aattttcaat cctcgagtta catttggctt acggcaatag ccaaaatcta tccacgcaac
    20821 aagaggcgtt ttcactaaac ccatgttgat agctttattt acaaaatagg ccttaaggtt
    20881 acataccaaa acatactctg gtgaccagta ctctgggttt tttaattgtc gaggctcaag
    20941 tctatttgta aacgactcat ctttttgaat tttttcgata cgacttctga tatatctaaa
    21001 tttctttttt atatcgataa cgataaccgt tgttggtttt ccgttgcgaa tcgcttcaat
    21061 ccttggtttc aaatcgggag aagtgaaaat aatcatctca ttttcaagtg cggccagacg
    21121 ctcaaagtaa ctaaaataaa catcaactga acgagctagt ttttcacgga accctttatt
    21181 agcagtccaa tcccctctac caatgtcaaa atatgcagtg ataattgtgg tggatgattt
    21241 cattactaat aatgaccatt aatataaaaa aagaattata tcataaatat taacagaatg
    21301 tatactgtat attatccatt caaatgattc cgttatttat catacatcgg aatattgata
    21361 ctaaagcact attcacatgc aaaaccaaca tccgccatga aggactagct aaaacccaaa
    21421 ctagtttgtt gcaattagca tccttgcacc tctatgtaaa gggctgaagg gattcggatg
    21481 tgatggtatg attacagaca ttcgtgtctg agattgtctc tgactccata attcgaaggt
    21541 tacagttatg atcatcgtta ccggcggcgc gggctttatc ggcagcaaca tcgttaaggc
    21601 cctgaatgat aaaggcatca ccgatattct ggtggtggac aacctgaaag acggcaccaa
    21661 gtttgtgaac ctggtggatc tggatatcgc ggactatatg gataaggaag acttcctgat
    21721 ccagattatg gctggcgaag agttcggtga tgtcgaagcg attttccacg aaggcgcatg
    21781 ctcttccacc accgagtggg atggcaagta tatgatggat aacaactatc aatactccaa
    21841 agagctgctg cactactgtc tggagcgcga aatcccgttc ctgtatgcct cttccgcagc
    21901 cacctacggc ggacgcacct ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa
    21961 tgtctacggt tactcaaaat tcctgtttga tgaatatgtt cgtcaaatcc tgccagaagc
    22021 gaactcgcag attgttggct tccgctattt caacgtttat ggaccgcgtg aaggccataa
    22081 aggcagcatg gcgagcgtcg ctttccatct caacacccag cttaataacg gtgaatcacc
    22141 gaagctgttc gaaggtagcg agaacttcaa acgtgacttc gtctatgtgg gcgacgtggc
    22201 agatgtaaac ctgtggttcc tggaaaatgg cgtttccggt atcttcaacc tcggcaccgg
    22261 tcgtgcagaa tccttccagg cagtagcaga tgctacgctg gcttatcaca agaaaggcca
    22321 gatcgaatac attccgttcc cggataagct gaaaggccgc taccaggcgt tcactcaggc
    22381 agatctgaca aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg
    22441 tgtaacggaa tacatggcct ggctgaatcg cgacgcataa gagctctgca tgaaaatact
    22501 ggtgatcggc ccgtcttggg ttggcgacat gatgatgtcg caaagtctct atcgcacgct
    22561 ccaggcgcgc tatccccagg cgataatcga tgtgatggca ccggcatggt gccgtccatt
    22621 attatcgcgg atgccggaag ttaacgaagc tatccctatg cctctcggtc acggagcgct
    22681 ggaaatcggc gaacgccgca aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc
    22741 ctacgtctta ccaaactcct tcaaatctgc attagtgcct ttcttcgcgg gtattcctca
    22801 tcgcaccggc tggcgcggcg agatgcgcta cggtttactc aacgatgtac gcgtgctcga
    22861 taaagaagcc tggccgctaa tggtggaacg ctatgtcgcg ctggcctatg acaaaggcat
    22921 tatgcgtacc gcacaagatc tgccgcagcc attgttatgg ccgcagttgc aggtgagcga
    22981 aggtgaaaaa tcatatacct gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg
    23041 cttttgcccg ggtgcggagt ttggtccggc aaaacgctgg ccacactacc actatgcgga
    23101 actggcaaag cagctgattg atgaaggtta tcaggtggtt ctgtttggct ctgcgaaaga
    23161 tcatgaagcg ggcaatgaga ttcttgccgc tttgaatacc gagcagcagg catggtgtcg
    23221 gaacctggcg ggggaaacac agcttgatca agcggttatc ctgattgcag cctgtaaagc
    23281 cattgtcact aacgattctg gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt
    23341 tgccctgtat ggtccaagta gcccggactt cacaccgccg ctatcccata aagcgcgcgt
    23401 gatccgtctg attaccggct atcacaaagt gcgtaaaggt gacgctgcgg agggttatca
    23461 ccagagcttg atcgacatta ctccccagcg cgtactggaa gaactcaacg cgctattgtt
    23521 acaagaggaa gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt
    23581 tctccatacg ttgcccgcac tcactgatgc ccagcaggca atcccaggga ttaagtttga
    23641 ctgggtggtg gaagaagggt tcgcacagat tccttcctgg cacgctgccg ttgagcgagt
    23701 tattcctgtg gcaatacgtc gctggcgtaa agcctggttc tcggccccca taaaagctga
    23761 acgcaaagcg tttcgtgaag cgctacaagc agagaactat gacgcagtta tcgacgctca
    23821 ggggctggta aaaagcgcgg cactggtgac acgtctggcg catggcgtaa agcatggatt
    23881 ggactggcaa accgctcgcg aacctttagc cagcctgttt tacaattgta agcatcatat
    23941 tgcaaaacag cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa gtttggacta
    24001 tagcaaaccg caaacccagg gcgattatgc tatcgcacag cattttctga cgaacctgcc
    24061 tacagatgct ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg ataaacactg
    24121 gccggaagaa cactggcgag aattgattgg tttactggct gattcaggaa tacggattaa
    24181 acttccgtgg ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg aaggatttgc
    24241 ttatgttgaa gtattgccga agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc
    24301 taaatttgta gtgtcggtgg atacggggtt aagccattta acggcggcac tggatagacc
    24361 caatatcacg gtttatggac caaccgatcc gggattaatt ggtgggtatg ggaagaatca
    24421 agtagagtgt cgttctacaa gtatgtctct tgcagatttg ccagctcaaa ccgtttttca
    24481 aaatttaaat cttgaaataa taaccaataa gttgacatcg gagataagat gacctcaaca
    24541 ttatttttct ctctcgagaa aaaaaactgg atagcgtact ggaacagagc tctcgtattc
    24601 ttattcatta ccacctattt tttgggtggg attacaaggt ataaacatct tattgttatt
    24661 cttatgacaa taacgacaat cgtctatctc tgcaaacggc caaaacacta tctctcacta
    24721 tttaaaacat ttctttttgg tagtgttgcc atattaacta ttgctgcatt gctgtcactt
    24781 cttcaatccc ctgatgcagg tgctagcatg aaggaaattt tcaaagctat tattgagaat
    24841 actttactat gcacaatagc aataccggtc atattgagag acgagaaaag agaagatgtc
    24901 gaaaaaatcg ttttcttctc atttattagt gcgttgggct tacgctgttt ttctgaattg
    24961 attacctatt ataaggacta tcaacaaggg ataatgccat tcgcagatta tagacaccgt
    25021 agcatttctg actcgatggt ctttttattc cctgcattgt taaatctctg gcttatcaaa
    25081 tcagcaaaat accgcatttc ttttgtggtt ctaagcgtta tttttatttt tctgatatta
    25141 ggaactttat ccagaggggc ctggctttcc gtgttagtca ttggattaat atggattctg
    25201 atgtttaaac aatggaagtt actattagta ggagtaatgg tttccatcat tgcattgtcg
    25261 gttattttca cacataagga gatgaccgca aagctaacgt ataaacttca acaaactaat
    25321 agttcttatc gctatgcaaa tggtactcaa ggcagcgcac tcgatctaat attagaaaat
    25381 cctgttattg gttatggtta cggtaacgtt gcatataaag atgtctataa taaacgtgtc
    25441 attgattatc cagaatggac ctttagacaa tcaatagggc cacataattt tgcgctattc
    25501 atctggtttg gcactggttt attagggctg gtaagtctta tgatgctata ctgtgcaata
    25561 ttgaaagagt gtataaaaaa tggcgtcaag aataaatatc gctcaccata taatgcatat
    25621 tatataatct tactatcttt tataggttat tttgttatcc gtggaaacgt agaacaaatt
    25681 gaaccaaatt tattaggcgt ttacgccggc ttattattag cgatgaaaaa caagtaaaaa
    25741 ataaaaaagg ctgcataatg cggccttttt atttattcaa accaattatg aataacctct
    25801 tcgaatcttt gagttacgcc atcccaactg tagtgatcaa acacaaagtc ctgaccttgc
    25861 tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatggag
    25921 tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc
    25981 atccccccac gcgtactcac taaaacaggc ttaccagcac ccatagcttc aattgccacc
    26041 atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag tgggtaataa
    26101 caatgcatct tttcaggcgg tatgcttcct aacataatgc aacgatcctt caaacgtttt
    26161 gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa
    26221 tcaccaatca caactaattt taaatttttg tgagcagcgg ccaatttttc gaatgattgc
    26281 atcaacagca atatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct
    26341 tcaggggaga tattaagttc gcttctagtg atcggctgaa aattagattg ataggtttct
    26401 aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac
    26461 tttttcaagt ataaactagg gactatcatt ttaacatttt gctcaagcaa ttttggttca
    26521 aatgcattat gcatatgtat aaccactctt gcctgaggag ctcgcttacg aatttgagtg
    26581 tatagcttca tgctattatg aactacaata acactttctt tcgttatagg aaaatcatgt
    26641 gcaatattca atattctctg tgaatatgga agaggatcaa gtcttgtcca tttttgaaat
    26701 aaccgcttat atatgcgact gaatccaacc ctatgaatac tacaattatc attaacaaag
    26761 gtgtaatttg agtatcctgg atttttgata caaacaatac gatttggaaa attggtacgc
    26821 tgagcaacct gatacatcca cgtttcaaca gcagccgctc cgcgaggagg aattgaaaaa
    26881 ataggagtaa ccgtaaatat tattttatca accatctcgt atattaacct ttcataacat
    26941 tatatttaat tgctgtaaaa acaccatcga gaaatttttt ctggtaaagc aagtgtttat
    27001 atttttcttt gtgctcatgt tttttaacag cttttttata aggtatattt ctccatggtg
    27061 atatattata aatattacga aaataatctg cagaggcgta gttcgcccaa tcatgccatg
    27121 gcttagttat accagtataa tgtatgaaga cagtgtcatc attaataaac cgggtgtaat
    27181 attcactatt tttttcttca aattctgact ttatagtata aatacaatta tattttctcg
    27241 gtaatatttt agcatgatgt agaaacatta agtttaaaat atcttgatcc gggtacttta
    27301 atttttttat aattgactca tctgataata gatcaaagaa tttttccgtt agtctttgtt
    27361 ttaaccattc ccgtaaatta atatacatta caccagagtt aaaatagcta ccgttaaaat
    27421 cctcattaca caaacgctca gcgcttttac tttgcatagc atctacatca attaccactg
    27481 ccccatattc atctttaaac tctaatgcta ttaactcgtt caatgaaccc ttacagacga
    27541 tatcagcatc aagatataat aatgaatcca atcgcgcaga aaaataatca aatgaaagca
    27601 aacgataata aatagatact ggccagatat ttgattgagg tagcgccttc aatggctcag
    27661 aatcaatatg atataattta attactgttt gatacttcgt tgctaattgt gataataatt
    27721 caatatactc tttgtctaag taatcagcga ttatgtgaaa ggtaaatttg ttttgtttat
    27781 tcttttcaag aattgaagca attgaaatcg cagccccaac ctggtagttc tcatcaatac
    27841 cccatgagac attaaacgtt tcccgttcat caagtttaac ggggcgcttg tccagttcaa
    27901 ttatatcttt aaattgagta agatgtttaa aatccaattt aattaccctt catcataatc
    27961 attatttgct cttcaatcct agtttgactt ttacatccct gattactttt ttaattttct
    28021 ttttgaatat aagatatgta tagccaaagt ctttcaattc attttttata ttatagtgcc
    28081 gttcaagatc aatgcgatct tttgctttta aaacagcggt tgttttttta cctgaaaggt
    28141 caattagtct taaacctttt tcggatacta taaaatttcc tttatgagga tcgccagaca
    28201 ccattccatg ctgatgcaac tcttttatcg attctgataa ttgatccttc agatcttcag
    28261 aaatttccaa atactcattt aacccgacac cttcaatata ctcaatgagc ataatataat
    28321 aatgagcgaa atttaatgtt ttacgttcag ctagcaaaaa atagtcatta attgattgga
    28381 tgccttcacc acgtacccga tcggtttgat aaattaaatt ttcataataa tcttttttta
    28441 tgcaagattt taagaaacgt tcggtcattt tatgctttgg cgcataaact ttaagtacaa
    28501 gaggtccacg agcagtgtca ataagaatga ctttagtatc atcaatagaa cgaaaaactt
    28561 ttaatgtttt aaccctgcaa gttaaaaagt catttagaac ttgctcataa aagggatcat
    28621 tatcttttat gaaaactttc
//
LOCUS       DADLXX010000038        28463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLXX010000038 DADLXX010000000
VERSION     DADLXX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.038"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="JNX27_001690"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="JNX27_001690"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7882932.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNX27_001691"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNX27_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7882933.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="JNX27_001692"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="JNX27_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7882934.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /locus_tag="JNX27_001693"
     CDS             2778..4127
                     /locus_tag="JNX27_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7882935.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="JNX27_001694"
     CDS             4278..5927
                     /locus_tag="JNX27_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7882936.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="JNX27_001695"
     CDS             complement(6081..7373)
                     /locus_tag="JNX27_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7882937.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNX27_001696"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNX27_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7882938.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVMLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIISFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="JNX27_001697"
     CDS             complement(9197..9511)
                     /locus_tag="JNX27_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7882939.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNX27_001698"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNX27_001698"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY7882940.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="JNX27_001699"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="JNX27_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7882941.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAVLDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="JNX27_001700"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="JNX27_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7882942.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="JNX27_001701"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="JNX27_001701"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7882943.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="JNX27_001702"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="JNX27_001702"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7882944.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="JNX27_001703"
     CDS             15750..17468
                     /locus_tag="JNX27_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7882945.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLAAFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="JNX27_001704"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="JNX27_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7882946.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="JNX27_001705"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="JNX27_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7882947.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="JNX27_001706"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="JNX27_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7882948.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="JNX27_001707"
     CDS             complement(20170..20859)
                     /locus_tag="JNX27_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7882949.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNX27_001708"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNX27_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNX27_001709"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNX27_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7882950.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNX27_001710"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNX27_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNX27_001711"
     CDS             complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNX27_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7882951.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGDDSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27862..28137)
                     /locus_tag="JNX27_001712"
     CDS             complement(27862..28137)
                     /locus_tag="JNX27_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882952.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28346..>28463)
                     /locus_tag="JNX27_001713"
     CDS             complement(28346..>28463)
                     /locus_tag="JNX27_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882953.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6485 a         7207 c         7641 g         7130 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcagtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctagagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag attatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tccttttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagctgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcatcaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg tgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaacttaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cggcgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgtgct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttcga gcgccgcacg ttgcgccacc agcgcatcga cgccgctgac
    27301 ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg
    27361 ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg
    27421 ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt
    27481 ggcattaacc gagtcgtcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat
    27541 ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg
    27601 cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag
    27661 ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc
    27721 gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc
    27781 caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc
    27841 catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc
    27901 aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac
    27961 tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata
    28021 ataagcgccg atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg
    28081 aattatccag gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt
    28141 aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg
    28201 tcagtatggc tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac
    28261 ttatatattt tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct
    28321 tcagcgcccc caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga
    28381 aattatccag acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc
    28441 tcttcgcttt caccagatcc gcc
//
LOCUS       DADLXX010000039        28092 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLXX010000039 DADLXX010000000
VERSION     DADLXX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.039"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..192)
                     /locus_tag="JNX27_001714"
     CDS             complement(<1..192)
                     /locus_tag="JNX27_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7882954.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
     gene            386..1549
                     /gene="yqhD"
                     /locus_tag="JNX27_001715"
     CDS             386..1549
                     /gene="yqhD"
                     /locus_tag="JNX27_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7882955.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
     gene            1654..2481
                     /gene="dkgA"
                     /locus_tag="JNX27_001716"
     CDS             1654..2481
                     /gene="dkgA"
                     /locus_tag="JNX27_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7882956.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDEPGKIAKLDQGKRLGPDPDQFGG"
     gene            2681..3607
                     /locus_tag="JNX27_001717"
     CDS             2681..3607
                     /locus_tag="JNX27_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7882957.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            3658..3915
                     /gene="yqhH"
                     /locus_tag="JNX27_001718"
     CDS             3658..3915
                     /gene="yqhH"
                     /locus_tag="JNX27_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7882958.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(3958..6177)
                     /locus_tag="JNX27_001719"
     CDS             complement(3958..6177)
                     /locus_tag="JNX27_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7882959.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSLINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(6288..7700)
                     /gene="ftsP"
                     /locus_tag="JNX27_001720"
     CDS             complement(6288..7700)
                     /gene="ftsP"
                     /locus_tag="JNX27_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7882960.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(7775..8512)
                     /gene="plsC"
                     /locus_tag="JNX27_001721"
                     /gene_synonym="parF"
     CDS             complement(7775..8512)
                     /gene="plsC"
                     /locus_tag="JNX27_001721"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7882961.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(8746..11004)
                     /gene="parC"
                     /locus_tag="JNX27_001722"
     CDS             complement(8746..11004)
                     /gene="parC"
                     /locus_tag="JNX27_001722"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7882962.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11550..12032)
                     /locus_tag="JNX27_001723"
     CDS             complement(11550..12032)
                     /locus_tag="JNX27_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7882963.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(12085..12477)
                     /gene="ygiW"
                     /locus_tag="JNX27_001724"
     CDS             complement(12085..12477)
                     /gene="ygiW"
                     /locus_tag="JNX27_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7882964.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            12629..13288
                     /gene="qseB"
                     /locus_tag="JNX27_001725"
     CDS             12629..13288
                     /gene="qseB"
                     /locus_tag="JNX27_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7882965.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLSSDFIRTVHGIGYTLGE
                     K"
     gene            13285..14634
                     /gene="qseC"
                     /locus_tag="JNX27_001726"
     CDS             13285..14634
                     /gene="qseC"
                     /locus_tag="JNX27_001726"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7882966.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLIGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLLALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14680..15012)
                     /locus_tag="JNX27_001727"
     CDS             complement(14680..15012)
                     /locus_tag="JNX27_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY7882967.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            15331..15912
                     /gene="mdaB"
                     /locus_tag="JNX27_001728"
     CDS             15331..15912
                     /gene="mdaB"
                     /locus_tag="JNX27_001728"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7882968.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            15943..16257
                     /locus_tag="JNX27_001729"
     CDS             15943..16257
                     /locus_tag="JNX27_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7882969.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(16305..18197)
                     /gene="parE"
                     /locus_tag="JNX27_001730"
     CDS             complement(16305..18197)
                     /gene="parE"
                     /locus_tag="JNX27_001730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7882970.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(18226..18807)
                     /gene="yqiA"
                     /locus_tag="JNX27_001731"
     CDS             complement(18226..18807)
                     /gene="yqiA"
                     /locus_tag="JNX27_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7882971.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(18807..19634)
                     /gene="cpdA"
                     /locus_tag="JNX27_001732"
     CDS             complement(18807..19634)
                     /gene="cpdA"
                     /locus_tag="JNX27_001732"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7882972.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(19659..20081)
                     /locus_tag="JNX27_001733"
     CDS             complement(19659..20081)
                     /locus_tag="JNX27_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY7882973.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(20082..20711)
                     /gene="nudF"
                     /locus_tag="JNX27_001734"
     CDS             complement(20082..20711)
                     /gene="nudF"
                     /locus_tag="JNX27_001734"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7882974.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            20916..22397
                     /gene="tolC"
                     /locus_tag="JNX27_001735"
     CDS             20916..22397
                     /gene="tolC"
                     /locus_tag="JNX27_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7882975.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            22545..23216
                     /locus_tag="JNX27_001736"
     CDS             22545..23216
                     /locus_tag="JNX27_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7882976.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            23222..24382
                     /locus_tag="JNX27_001737"
     CDS             23222..24382
                     /locus_tag="JNX27_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7882977.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(24420..25235)
                     /gene="ygiD"
                     /locus_tag="JNX27_001738"
     CDS             complement(24420..25235)
                     /gene="ygiD"
                     /locus_tag="JNX27_001738"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7882978.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            25351..26124
                     /gene="zupT"
                     /locus_tag="JNX27_001739"
     CDS             25351..26124
                     /gene="zupT"
                     /locus_tag="JNX27_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7882979.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(26182..26352)
                     /locus_tag="JNX27_001740"
     CDS             complement(26182..26352)
                     /locus_tag="JNX27_001740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7882980.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(26613..27266)
                     /gene="ribB"
                     /locus_tag="JNX27_001741"
     CDS             complement(26613..27266)
                     /gene="ribB"
                     /locus_tag="JNX27_001741"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7882981.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(27371..27519)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27581..27931
                     /gene="ubiK"
                     /locus_tag="JNX27_001742"
     CDS             27581..27931
                     /gene="ubiK"
                     /locus_tag="JNX27_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7882982.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT         7095 a         7454 c         6954 g         6589 t
ORIGIN      
        1 attgatataa ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac
       61 cggtgtacgt gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga
      121 caaactattt tctttatttt tcagtttatt aactttatcc gccagcaagc gacaaatctc
      181 ttcacgtttc atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt
      241 ttgtagcatt tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg
      301 agagccaggg cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat
      361 attggcccag caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat
      421 tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt
      481 attgattacc tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga
      541 tgccctgaaa ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga
      601 aacgctgatg aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt
      661 tggcggcggt tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga
      721 aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc
      781 gatgggctgt gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat
      841 ctcccgtaaa accacaggcg acaagcaggc gttccattct gcacatgttc agccggtatt
      901 tgccgtgctc gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt
      961 agtggacgcc tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat
     1021 tcaggaccgt ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct
     1081 gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct
     1141 gaacggtttg attggcgctg gcgtaccgca ggactgggca acgcatatgc tgggccacga
     1201 actgactgcg atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact
     1261 gtggaatgaa aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg
     1321 gaacatcact gaaggttccg acgatgagcg tattgacgcc gcgattgccg caacccgcaa
     1381 tttctttgag caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc
     1441 catcccggct ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca
     1501 tgacattacg ttggatgtca gccgccgtat atacaaagtc gcccgctaag ctttttacgc
     1561 ctcaaacttt cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc
     1621 ctgctccggt gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta
     1681 caggatggca atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa
     1741 gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg
     1801 gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa
     1861 gaactgttca tcaccactaa gctgtggaac gacgaccaca agtgcccccg cgaagccctg
     1921 ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc
     1981 gttcccgcta tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag
     2041 ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt
     2101 gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa
     2161 cgccagctac acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta
     2221 gcgcaaggag gaaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac
     2281 ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc
     2341 ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc
     2401 gacaaagacg aacccggcaa aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat
     2461 cctgaccagt tcggcggcta acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg
     2521 gacttttcag aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca
     2581 ggaatgccaa atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca
     2641 tctatatccg ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt
     2701 tttagcagcc ctggcaagtt ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca
     2761 aacagtccgg cagatttatc agaactataa atcagatgcc agtacgcctt atttcggtga
     2821 aaccggagag cgggcgataa cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa
     2881 tcttacacta ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga
     2941 ttttggcgat ctggtgttag aaagcgtagc gataacccaa actgacgtcg atcatgccga
     3001 tgccgttgtg cgctttcgta tctttaaaga tgataaagaa aagaccacac agacactgaa
     3061 aatggtggcg gaaaatggtc gttgggtcat tgatgatatt gtcagcaatc atggcagcgt
     3121 cttacaagca gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcagaa
     3181 agaacagcca gaatcctttg ttgctgaact ctttgaacat attgctgatt atagctggcc
     3241 gtggacgtgg gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac
     3301 cttcaagacg gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga
     3361 caatccgatc tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct
     3421 ggagaaaacc gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa
     3481 tgaagagcaa gagctggttt tacagcggcg cgaaggtaag tgggaaatcg ctgattttat
     3541 ccgcccgaac agcggcagcc tacttaagct gattgaggca aaaaccgccg ccagattaaa
     3601 gcaatgagct gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg
     3661 aaaacgattt tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc
     3721 aatgagcaaa aggtcaatca actggcgagc aatgtacaaa cattaaatgc caaaatcgcc
     3781 cggcttgagc aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct
     3841 aacagagcca atacgcgtct tgatgcacag gactattttg attgcctgcg ctgcttgcgt
     3901 atgtacgcag aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta
     3961 acgtccaacc gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt
     4021 agacgacgct ttttttgccg tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt
     4081 agtcaacgcc ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt
     4141 tggcgcaggc actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc
     4201 cagcgcctgg cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg
     4261 caaacgacgc tgtttgtcgc ccttcggtac gaagacgtct tcgctcttat aaccaatctt
     4321 cgccagcggt tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta
     4381 gaagttctgt acctggtcga gacggaaacg atgctttttc agccacagcg ccaaattcac
     4441 catatcttca tcacgcgtac cggggtgcgc agagatgaaa tacgggatca gatactgctc
     4501 tttacctgcc tgtttcgagt aggtatcgaa cagctcttta aagcggtcat agctgcccat
     4561 gcccggcttc atcatcttcg acaacggccc ttcttcggta tgttccggag caatcttcag
     4621 ataaccgccg acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat
     4681 gtcataacgt acgcctgagg cgataaggat ttttttaatg cctttcagat cacgcgcacg
     4741 gcgatagagg ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata
     4801 aacgcacgac aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata
     4861 catgttggca gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc
     4921 gcggatcgct tcgatctcat taatgagcga atcttccgaa cggctctgaa taatgcgccc
     4981 ttcgtgctcg gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac
     5041 cgaaaaacgg atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac
     5101 gcgcttgtac ggcagcgcaa aaacgcagtc catctcttcg gtagaaagcg ggatagcagg
     5161 cgggttgatc cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg
     5221 gttggtttcg tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac
     5281 tttctcgaaa gaaggcagca atacgtaggt tttttcccac ggtttctggc gcggtggctg
     5341 cacgattaca gctttcgctt cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg
     5401 caaatcttcg ccatacggat gcgggattgg gtcgattttc cctggggtat caagacgggt
     5461 ggaatccaca ccgctccagc caggcagcgc ctcttttacg ataattgcgg tattacgcac
     5521 atcgcggatt tcactaatcg gctcgcccat cgccagacga tgcgctactt ccaccagcgg
     5581 acgttcaccg ttaccaaaca tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg
     5641 cacggtatcg gaccagtaat cataatgcgc ggtacggcgc aggctagcct caataccgcc
     5701 gaggatcacc ggcacatctt tccacgcctc tttacaacgc tgggtataaa ccagcgtggc
     5761 gcgatccggt cgcttacccg cgacattatc tggcgtgtag gcatcatcat gacgtaaacg
     5821 gcgatcggcg gtatagcggt tgatcatcga atccatgttg ccagcagtaa caccaaaaaa
     5881 cagattcggt ttacccagac gcataaagtc gtctttgctg ctccagtccg gctgggcgat
     5941 gatcccgacg cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct
     6001 tgggtgatcg acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag
     6061 ttgatccatc tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca
     6121 gtactgcggc caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt
     6181 gcttccagaa atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag
     6241 aaatcgaaag gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga
     6301 ttgaccaaca gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag
     6361 tagaacggga agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg
     6421 tcaacccaaa cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg
     6481 ttacggatct ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc
     6541 gtccagcgtt cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc
     6601 tgtccattaa tacccgggtc atcacccaga ctgatatcgc gactgcgaat tggcgaacca
     6661 gccatgattt cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg
     6721 ccggttgggc gcagcgttag caccaaggta gaaaccagaa tactggatgg ctcaaagaag
     6781 ccgcgaatac gatcaacaat gctcgccgct tcgccacagg tgatcgacac ttcatcgccg
     6841 ttgctcatat ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc
     6901 gatacaggag caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg
     6961 ctcatctgta gttgatagcg acgagagttc gacgcgttca gcagtcgcaa gcgcacccag
     7021 ccacgcgaga cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca
     7081 aagccgccgc ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc
     7141 tggataatga ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg
     7201 acttcatctt ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg
     7261 ttgggagtat tggcgtgata ccacagagta gctgcgttct ggcgaatagg cagtacgggt
     7321 gcccagtcag cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc
     7381 tgtagcccgg ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta
     7441 acatcgtcgc ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag
     7501 accgacgcgc gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc
     7561 ggttgcccac ggcgagattc aagtagcggc ggaacgggta gcggttgctg ttgcccggct
     7621 gcgctggcct tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac
     7681 tgacgccgac tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag
     7741 gaaagtgttc cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt
     7801 ctgcgacttc tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag
     7861 ccagctcacg aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga
     7921 caatcaccag accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc
     7981 acacgggaat aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg
     8041 gtagcaggcc gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc
     8101 gttttttgaa atgattcacg acttccgcaa tggtgccgtg agctttagtg cgattgtttc
     8161 tgtcgatcaa taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca
     8221 agctcttttt acctaccgtc accgtcggcg gttgcacaat gttcgatgct gtcaccatgt
     8281 catagttgtt ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag
     8341 gtttgcggca ctcaactttc aggccgaaca gtggcgcaag acggccaaac atatgcccaa
     8401 aggtggccac atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac
     8461 agactaagat gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct
     8521 ctgaaggtct gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa
     8581 agccgctctc tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag
     8641 ggaattttta tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc
     8701 cggcgttcct tgctagcggg aggaaacagc gccctccccg gcatattact cttcgctatc
     8761 accgctgctg gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg
     8821 catcaacgta ccgcggcgtc cacgttcgcc agtgactttc tgtagctctt ccggacgcag
     8881 tttaattttg cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag
     8941 ttgcgccaga ccatcctcgc cacgtgcggc ttctgccgat ggaatgttga taatcttgtt
     9001 gcctttgccc ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc
     9061 ctgagtgatt gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg
     9121 ggcattttcc ggtaaggtga tcaaagcctt acccgcacgg ttacgcgcca ccagatcgtt
     9181 aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct
     9241 ttccatcagc atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg
     9301 ttcgccctga ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt
     9361 ggaatcaaca aacactaccg gttggttact cttacctttc accgccgctt tgaagctatc
     9421 acccgcttta taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca
     9481 gcccatctgc gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat
     9541 agctttcgct tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc
     9601 gtctgcctgc agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc
     9661 ctgcaactgg tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc
     9721 cagtttggca agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag
     9781 gccaaaccgc gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac
     9841 ttcgtcgatg ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac
     9901 tttctccaga cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc
     9961 ggagaggatt tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag
    10021 gttaatgcga tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc
    10081 catatccacg cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga
    10141 ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg
    10201 ctccagtacg cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc
    10261 ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg
    10321 aatctcggcg cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat
    10381 atccagcagc tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc
    10441 tacttcacgc aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt
    10501 gccgttaagc aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc
    10561 gtcgaagttt ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga
    10621 atatttcgac aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg
    10681 cgcgccccag ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat
    10741 caggaccatc gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc
    10801 accgacagta cgggccgatt ttttaaattt ggctctggca ttcaggccca gttcagacat
    10861 cgcatacaca atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg
    10921 gtccatgatc acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc
    10981 aaggcgctct gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa
    11041 aggggcaata ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg
    11101 tgaatagcgc cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac
    11161 aactctttgc tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca
    11221 ccactcggca tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg
    11281 gaagcctaaa gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg
    11341 tctccaccag tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tacccccgag
    11401 acggcttacg gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa
    11461 attataagcc caacaacaat acgcctgtga cattgttcgc tcaaacttat cgcgagtgat
    11521 ttggctcaca ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc
    11581 agtcgctgct ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta
    11641 cgcattttct cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa
    11701 atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca
    11761 ttgctcacac cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag
    11821 cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt
    11881 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag
    11941 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    12001 ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    12061 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    12121 ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt
    12181 caccgtcaca ccgttccagc gtttgtgatc gatatcaaca ttgatagtac cgctggcatc
    12241 tttaaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca
    12301 ggtgtcgtca cgcagggatt ttacgctttc tacagtcgtt acgctgccgt tcggcccctg
    12361 aaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc
    12421 catcaccggt gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    12481 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    12541 aagggaaaaa aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag
    12601 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    12661 gattggtgac ggcatcaaaa ctggccttag taaaatgggt tttagtgtcg actggtttac
    12721 acaaggtcgt cagggaaaag aggcgcttta tagcgcacct tatgatgcgg tgatcctgga
    12781 tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag agaaaggtca
    12841 gcgtgagccg gtactgaacc tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct
    12901 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    12961 gctggaggct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa
    13021 cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa
    13081 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    13141 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    13201 agtgcatgtg catcatctgc gacgcaaact cagtagtgat tttattcgta ccgtgcatgg
    13261 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg
    13321 ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc
    13381 tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc
    13441 aaacggttaa gtacgcccga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg
    13501 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc
    13561 cacgacggca gaatggtcct taatgatggc gataacggag aagatattcc ctatagctat
    13621 caacgggaag gttttgctga cgggcaactg atcggtgaag acgatccttg gcgttttgtc
    13681 tggatgacct cacctgatgg caaatatcgc atcgttgttg gccaggaatg ggaataccgt
    13741 gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggctgct cgcactgccg
    13801 attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg
    13861 gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta
    13921 cccagcgaag tgcggccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg
    13981 atgatggttc gagaacgacg ctttacctcc gatgcagctc acgaactacg tagcccgtta
    14041 acagcgctga aagtgcaaac cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg
    14101 aaaaaagcac tgctccaatt acattccggg atcgatcgcg ctactcgtct ggttgatcaa
    14161 ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    14221 cttgaagatc tcctacaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa
    14281 attgacgtgc gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg
    14341 ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc
    14401 gtgatagacg tcacgctgaa tgctgataat ttcatcgtga gggataacgg ccccggtgtg
    14461 acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg acaaaccgct
    14521 accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaagctgca tgacatgaat
    14581 gttgagtttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg gtaacttctc
    14641 tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa atataaaccg
    14701 tccccaaaaa agccaccaac cacaaaccag acaaataacg agcgagaata catatagcca
    14761 aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa gtggcgtaag
    14821 aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat
    14881 actgcaaata ttgcctcccg actcaagcat ccagtcatac ttattgaaag caaaaaaaga
    14941 atctattaac caataagtaa gcattatata tagcaatgct tcaaatattg ttttaatttt
    15001 ctgctttaac atatatcagt acgctcataa aaatccattt ataattatca acatgacacg
    15061 ttgatatttt catcttttac ttattcaaag ccatattttc aatccccaaa aaaaccgaac
    15121 caaacgtgta aattattccg gtacgagtaa gaattatgag gaatggctat cagtattgtc
    15181 attttcagaa aatatttatc ctgcatcggt gagtcagagc tagatcagac ttttgctaaa
    15241 ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac cgctctgtga cgtactatag
    15301 tcgacaaacg tctcaccttg aggttaaaaa atgagcaaca tcctgattat caacggcgcg
    15361 aaaaaattcg cccactccaa tggtcaactg aacgacaccc tgaccgaagt cgcggatggc
    15421 acactgcgcg acctcgggca tgatgtccgc atcgttcgcg ccgacagcga ctacaatgtc
    15481 aaagcggaag tacaaaactt tctctgggct gatgtggtga tctggcagat gccaggctgg
    15541 tggatgggcg cgccgtggac agtgaaaaaa tatattgatg atgtattcac cgaaggtcac
    15601 gggactctgt atgccagcga tggtcgtacc cgcaaagatc cgtcgaaaaa atacggttcc
    15661 ggcggcctgg tacagggcaa aaaatatatg ctttctctaa cctggaacgc accaatggaa
    15721 gccttcaccg aaaaagatca gttcttccac ggcgttggcg ttgacggtgt gtatctgccg
    15781 ttccataaag caaaccaatt cctcggtatg gaaccgctgc cgacatttat cgctaatgac
    15841 gtgataaaaa tgcctgatgt tccccgctat actgaagaat atcgcaagca tcttgtggaa
    15901 atttttggtt aactagagct caggctttag aaggagttaa ccatgcttac cgtaatcgca
    15961 gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca gtttgctaaa
    16021 atcgttccaa ccgtactgaa agaagaaggt tgccacggct atgcgccaat ggtggattgc
    16081 gctgctggcg tgagtttcca gtctatggca ccggattcta tcgtgatgat tgagcagtgg
    16141 gaaagcatcg cgcaccttga agcgcatctg caaaccccgc acatgaaggc gtacagcaaa
    16201 gccgtaaaag gcgacgtgct ggagatgaat atccgtattc tgcagccagg gatttaatcc
    16261 tgccttgttt gcccggccat cctgaccggg caatgttctt tcctttaaac ctcaatctcc
    16321 gccatgtcgc ctttctcttg caaccagttg cggcgatctt ccgagcgttt cttcgccagc
    16381 agcatatcca tcatcgcgtc agtacgctga tcgtcttcat catcgatagt caactgcacc
    16441 agacggcgag tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt catttccccc
    16501 agacctttaa aacgctggac gttcggcttg cctttcttgc gttttaattg ctcaagtacg
    16561 ccctctttct cttcttccgt cagcgcgtaa taaacctctt tcccgagatc aatacggtag
    16621 agcggtggca gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa gtgttttacg
    16681 aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag agtccgcatc cgcgaggata
    16741 cagattttgc cataacgaag ctggctcaga tcgtcgctgt caggatcgat accgatcgct
    16801 accgaaatat catgcacttc ctgcgaggcc agcacttcgt cggaagaaac ttcccaggtg
    16861 ttaaggatct tacctttcag cggcatgatc gcctgatatt cacgatcgcg cgcctgcttg
    16921 gcagatccgc ctgcggagtc accttccacg aggaacagtt cggtacggtt aaggtcctgc
    16981 gcggtacaat cagccagttt gccaggcagc gccgggccgc tggtcagctt tttacgcacc
    17041 acttttttag ccgcacgcat acgacgctgg gcgctggaaa tcgccatctc tgccagcaac
    17101 tccgccgcct gaacgttctg gttcagccac aggatgaagg catctttcac cacgcccgaa
    17161 acgaatgccg cacactgacg cgaagagaga cgctctttgg tctgcccggc aaactgcgga
    17221 tcctgcattt taaccgacag aacataggcg cagcgatccc agatatcttc cgccgacagc
    17281 ttcacgccgc gcggcagaat gttgcggtat tcacagaact cacgcatcgc gtccaacagg
    17341 ccctgacgca gaccattaac atgggtaccg ccctgcatcg ttgggataag gttgacgtag
    17401 ctttcggtca gcagttcacc gccttccggc agccacagta gcgcccagtc cacagcttca
    17461 gtatcgccag cgaaattacc gataaacggt ttttctggca gcgtcggtaa accgtttacc
    17521 gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc agcgctgttc ggtgttgttg
    17581 atctcatctt taaaggtaat ctcaacgccg gggcacagta ctgctttggc cttcagcaca
    17641 tgcgtcaggc gtgaaacaga aaaacgcggg ctgtcaaaga aggtttcatc cggccagaag
    17701 tgcacgctgg taccggtatt gcgtttaccg caagtgccga caacctgtaa atcctgcacc
    17761 ttttcgccat tttcaaaggc gatgttataa acctgaccat cgcggcgcac gttaacttct
    17821 acgcgcttcg acaaggcgtt aactaccgaa atccccacgc catgcaggcc gccagagaac
    17881 tggtaatttt tgttagagaa tttaccgcct gcatgcagac ggcaaagaat cagttcaacc
    17941 gccggtactc cttcttccgg gtgaatatcc accggcatcc cgcgcccatc gtcaatcact
    18001 tctaacgact ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc cgccagtgct
    18061 tcatcgacac tgttatcaat aacttcttgc cccaaatggt tagggcgagt ggtatcggta
    18121 tacatccccg gacggcggcg aaccggctca agcccggtga gtacctcaat ggcatcagcg
    18181 ttataagttt gcgtcatggt ttaagttagc aattcgagtt gatcgtcaga gatggtgcag
    18241 accaagaaaa tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg cgtggttgcc
    18301 gccttctatg acagtctggc ggcaggaagc gtagtacgcc accgcctggc ggtaatccag
    18361 cacttcatct cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca gcgggtcaat
    18421 ctgcatgact ttgagatcgt aaatatggcg tgactctagc acatattgct gcccggtgta
    18481 ggggttctcg ttctgaccga gatagtccgt cagcaattca aacgggcgta ccgccgggtt
    18541 taccaccact gcgggcagca taaaacattg cgacaaccag gtggcgtaat atccccccag
    18601 tgacgaaccg acaataccca gcgaatcgcc gccatgttcc aggacaatgg attccagcag
    18661 ttctgccgcg tcggaaggat acggcggcaa ctgcggaatg atcatctcaa cgtcagggtg
    18721 atgttccgcc agccagtttt ttaacaagct cgcttttgca gagcgcggcg agctgttgaa
    18781 accgtgtaaa taaagaagcg tagacatcag tagccttctg aagcggtatc aggttggaaa
    18841 cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg taactcgaga
    18901 gtacgccagc cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg cttaaactgc
    18961 acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc cagctcctga
    19021 tgaatatgac cgcacagcaa gtatttgacg tgcggaaact tcgccagcac ggtatccagt
    19081 tcgcccgcgt tacgcagact gtgttgatcg agccaactac aacccgcagg tagcggatga
    19141 tgatgcagca gcagcaacgt atggcgttct ggcgcatcgg ccagtttacg ttccagccac
    19201 tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa acacctggct atccagcaac
    19261 aggatttgcc attgctcacc aataaacacc cgcttcgccg gggagatacc cgcatcctgt
    19321 aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg gcagccagac gcagggcgca
    19381 cgaaaacttg cgatgccttc agcgaaatgc tgataggccg cagaggattg atcctgcgct
    19441 aaatcacctg tcgcgacaat caggtcgaat tcgtgctggt gtggacgaat cgcctccagc
    19501 accgcctggt agctttccca ggtgtttacc cctaacaggg cttcgtgctt ttgtgcaaac
    19561 aggtgagtat cggtaatttg taaaatcctt cctctggcct caccagccag aggaagggtt
    19621 aacaggcttt ccaaatggtg tccttaggtt tcacgacgct agtaaaccgg aatcgccatc
    19681 gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa actggttaat ttgatgcttt
    19741 tcgtcgcgtt gatgcaactt tttattagga taatcatacc gcgctttgaa gcgaaaaatc
    19801 tgctggcttg aacacacttc agccaccatc gcgtcatgat acagacgcac cgtcattgac
    19861 ggaaggctcc agtaactgat cgcgggcgca gtctgttcta ttgtcaccag ggtagtgtat
    19921 cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg ccacctgata gcttacagtt
    19981 tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt catctcgcac
    20041 aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca tttatgccca ctcatttttt
    20101 aacgcttgat gatgcagctg cagccattgc aaagcgatga ccgacgctgc gttgtcgatt
    20161 ttcccctctt ctacccactg gtatgcctgt tcccggctta ccacatgaac gcgaatatct
    20221 tcgttttcat cagccagacc atgaataccg cttgcggtcg tggcgtccac ttcgcccacc
    20281 ataattgacg aacgctcact ggtgcccccc gggcttgcca ggaaacttaa caccggtttg
    20341 gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac atcttccaca
    20401 ctttcaccct cttcaatcat cccggcaacc atctccagta gccagggggt ttcgctggtg
    20461 tcgtacgcgg caatccgaat ctgctcaatc agcacaactt catcacgcac tgggtcaaag
    20521 ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc gccgtacctc atgactcatt
    20581 tgcccgttga atagacgatg acgaaatcta taaagatcta atgaaaaaaa gccgcgataa
    20641 agtgtttctc gtgcaataat ttctacatcg tttttgccaa atgtaacggg caggttgtct
    20701 ggcttaagca ttgttaatgt cctggcacta atagtgaatt aaatgtgaat ttcagcgacg
    20761 tttgactgcc gtttgagcag tcatatgtta aattgaggca cattaacgcc ctatggcacg
    20821 taacgccaac cttttgcggt agcggcttct gctagaatcc gcaataattt tacagtttga
    20881 tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc cccattctta
    20941 tcggcctgag cctttctggg ttcagttcgt tgagccaggc cgagaacctg atgcaagttt
    21001 atcagcaagc acgccttagt aacccggaat tgcgtaagtc tgccgccgat cgtgatgctg
    21061 cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc acagctaggt ttaggtgcag
    21121 attacaccta tagcaacggc taccgcgacg cgaacggcat caactcgaac gcgaccagtg
    21181 cgtccctgca gttaactcaa tccatttttg atatgtcgaa atggcgtgcg ttaacgctgc
    21241 aggaaaaagc agcagggatt caggacgtca catatcagac cgatcagcaa accttgatcc
    21301 tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt tcctatacac
    21361 aggcacaaaa agaagcgatc taccgtcaat tagatcaaac cacccaacgt tttaacgtgg
    21421 gcctggtagc gatcaccgac gtgcagaacg cccgcgcgca gtacgatacc gtgctggcga
    21481 acgaagtgac cgcacgtaat aaccttgata acgcggtaga gcagctgcgc cagatcaccg
    21541 gtaactacta tccggaactg gcggcgctga atgtcgaaaa ctttaaaacc gacaaaccac
    21601 agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg ttacaggcac
    21661 gcttgagcca ggacctggcg cgcgagcaaa ttcgccaggc gcaggatggt cacttaccga
    21721 ctctggattt aacggcttct accgggattt ctgacacctc ttatagcggt tcgaaaaccc
    21781 gtggtgccgc tggtacccag tatgacgata gcaatatggg ccagaacaaa gttggcctga
    21841 gcttctcgct gccgatttat cagggcggaa tggttaactc gcaggtgaaa caggcacagt
    21901 acaactttgt tggtgccagt gagcaactgg aaagcgcaca tcgtagcgtc gtgcaaaccg
    21961 tacgttcctc cttcaacaac attaatgctt ctatcagtag tattaacgcc tacaaacaag
    22021 ccgtagtttc cgcgcaaagc tcattagacg cgatggaagc gggctactcg gtcggtacgc
    22081 gtaccattgt tgatgtgttg gatgcgacca ccacgctgta caacgccaag caagagctgg
    22141 cgaatgcgcg ttataactac ctgattaatc agctgaatat taagtcagcc ctgggtacgt
    22201 tgaacgagca ggatttgctg gcactgaaca atgcgctgag caaaccggtt tccactaatc
    22261 cggaaaacgt tgccccgcaa acgccggaac agaatgctat tgctgatggt tatgcgcctg
    22321 atagcccggc acccgtcgtt cagcaaacat ccgcacgcac taccaccagt aacggtcata
    22381 accctttccg taactgatga cgacgacggg gcttcggccc cgtctgaacg taaggcaacg
    22441 taaagatacg ggttatctgc cgcattcttc ccccttctcg cttcaatttc gaccagccat
    22501 cctctattct gatgggtatt taccactggt cccggaagac aaaaatgaaa cggacaaaat
    22561 ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg ccatctgaca ccagtcgctc
    22621 tcgcggttgc cactgttttt atgctggctg gctgtgaaaa gagtgatgaa acagtgtctc
    22681 tctatcaaaa tgctgacgac tgttcagctg caaacccagg caaaagcgcc gaatgtacca
    22741 ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc gccgaaatac gccacccgtg
    22801 aagactgtgt tgctgaattt ggtgaaggtc agtgtcagca agcaccagcc caggctggca
    22861 tggcaccaga aaaccaggcg caggctcagc aatccagcgg gagtttctgg atgccgctga
    22921 tggccggtta catgatgggg cgtctgatgg gcggcggcgc gggatttgca cagcagccgc
    22981 tgttctcctc gaaaaacccg gccagtccgg cttacggtaa atataccgac gcgacgggta
    23041 aaaactatgg cgcagcccag ccaggccgca ccatgaccgt accgaagacg gcaatggcac
    23101 caaaaccggc gaccaccact accgttaccc gtggcggttt tggtgaatct gttgccaaac
    23161 aaagcactat gcagcgtagc gcaaccggca cctcttctcg ttcaatgggt ggctgatacc
    23221 gatggaaaga gtcagtatta ccgagcgccc ggactggcgt gagaaagccc acgaatacgg
    23281 tttcaatttt cacaccatgt acggcgagcc gtactggtgt gaagatgctt actacaagtt
    23341 gaccctcgcc caggttgaaa agctggaaga agtcaccgcc gaactgcacc agatgtgcct
    23401 gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg accaaattcc gcataccaaa
    23461 acacacctgg agttttgtgc gtcaatcatg gctgactcac cagccatcgc tttattcgcg
    23521 tcttgatctg gcgtgggatg gcactggcga acctaaactt ctggaaaata acgccgatac
    23581 gccaacgtca ctatacgagg cggcgttctt tcagtggatc tggctggaag atcagcttaa
    23641 cgccggtaac ttgccggagg gcagcgacca gtttaacagt ctgcaagaaa aactgatcga
    23701 tcgcttcgtt gagctgcgtg aacagtgtgg cttccagttg ctgcatctca cctgctgtcg
    23761 cgacacggtg gaagatcgcg gaaccattca gtatttgcag gactgcgcaa ccgaagctga
    23821 aatcgccacc gagttcctct acatcgacga gataggttta ggcgaaaaag gccagttcac
    23881 ggatttacag gatcaggtga tttctaacct gttcaagctt tatccgtggg aatttatgct
    23941 gcgcgaaatg ttctccacca agctggaaga tgctggcgta cgctggctgg aaccggcgtg
    24001 gaagagtatt atctccaaca aggcacttct accgctactg tgggagatgt tcccgaatca
    24061 cccgaacctg ctgcccgctt attttgcgga agatgatcat ccgcaaatgg aaaaatatgt
    24121 ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg atcattgaga acggcaaaac
    24181 cattgaagca gcggaaggtc cgtatggcga agaagggatg attgttcagc aattccaccc
    24241 gttaccgaaa ttcggcgaca gctatatgct gattggtagc tggctggtga acgatcaacc
    24301 tgccggaatt ggcattcgtg aagaccgtgc gttgatcacc caggatatgt ctcggtttta
    24361 tccacatatt tttgttgaat aagccacgat accggatggc actcgccatc cggtaattgt
    24421 tagcctatct gcaccgacag catacttaaa ctgcccattt ctataccatc gaccggaatg
    24481 gtaattggct cctgcccatc ccacacacct aacacataca acaacggcag atagtgctct
    24541 ggcgttgggt tcgataacgc gccaccttca tggtcgagat aattcaccag aggatgctgc
    24601 tctaccggcc cttgccacgt cagattcgct ttcacatact cattaaacga cgtcgcccac
    24661 ggatacggtg aactatcacc gtgccacttc actgtgcgca ggttatgcac cacgttaccg
    24721 ctggcgacca acattattcc ttcatcacgc aacgccgcga gtttgcgccc catttcgaaa
    24781 tgccaggcgg caggtttgct actgtcgata ctcaactgca ccatagggat atcggcgtca
    24841 ggatacatct taatcagcac gccccacgag ccgtggtcaa agccccaggc ttctttatcc
    24901 agcgtcaccg ggatcggcgc taacagctca accagacgct gtgccagcgc aggcgaaccc
    24961 ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg
    25021 ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc cgaaaccacc
    25081 acaatcgctt gcgggcgtgg caatgtcatc cccaacttct gccagctgcg ggtatacaaa
    25141 ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg catacgtgtt
    25201 gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca atacgcttgt
    25261 tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt gacgatctgc
    25321 ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct gaccatactg
    25381 gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcggacaaaa accctcgaac
    25441 cgcttactgg cgttttcgct aggttttgcg gcggggatca tgttgctcat ctcattaatg
    25501 gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc cggtgttggg ttatggaatg
    25561 tttatcttcg gtctgcttgg ctactttggc ctggaccgca tgttgccgca tgcgcatccg
    25621 caggatttaa tgcaaaaatc ggtgcagctg ttgccaaaat cgatcaagcg cacagccatt
    25681 ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac ctttgtcacg
    25741 gcgagcagca acctggagct gggatttggc atcgcactgg ccgtcgcgtt gcacaatatc
    25801 cctgaaggtc tggcagtggc aggcccggtt tatgcggcaa cggggtctaa acgcaccgcg
    25861 attctgtggg cggggatttc tggactagca gaaattcttg gtggtgtgct ggcgtggtta
    25921 atcctcggta gcatgatttc cccggtggtc atggcggcaa tcatggcggc ggttgcagga
    25981 attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat tgaccctaat
    26041 aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag tttagtgctg
    26101 ctacaaacgg cgggaattgg ttaaaaacaa catcagaagt tgtccagcac atggacaact
    26161 tctgccagct tcacgaaagc cttattctac cttttcggct gtctcttctc tcgtactgtt
    26221 taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg cccgacaata
    26281 acgtttcaaa tgcaggaaat aacccaccac aaccagagca atcaatacaa tccagtacca
    26341 ggcgataaac atccttttac ccttaatatt taacaatact aaacatttag cgcataaatt
    26401 tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca attatatgat
    26461 tttgatcata atgaaaaaga atgcctgata ttaatgaaat tttctcatag aaagagaagc
    26521 ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac aacttcggtt
    26581 tcttgattaa ggcagtaatt aagcagcggt tttcagctgg ctttacgctc atgtgcctga
    26641 cggtatgcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt ggcaaactca
    26701 atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca
    26761 gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg cttcagtatg accgccacgc
    26821 gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt ggcccgggcg attcagatca
    26881 gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacgatc agcggcagaa
    26941 acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt gccataggcg
    27001 ctggtgttat tttctaccat cattggcaga tcaagttgtt tacggcgatc ttcagtgatg
    27061 cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc aacggtcatg
    27121 gtttctgccg ggaagatcat atcgccttcg ttttcacggt cttcatcatc aagcaccatt
    27181 acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca
    27241 aaagaggaaa gtagcgtctg attcatggta aaaaaaacct cactaaaatt atggttacca
    27301 gaatcagggc agtcttagga gtggcggcat atagccaaaa taacgtgagc gggtccatgc
    27361 ccgacagaat cgttactctc tcccatccgg actctaaccg tcggccccgg aattacaccg
    27421 gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact gagttgattt
    27481 accgccggtg gggaatttcg ccccgccctg agaataagcg gattcactat aactctaatg
    27541 attagcggca gcaacgcata gcttcacata attctggttt atgacttacc cttatcgcac
    27601 tacaatggta ctcaacacct atcagtacag ggaaactaca atgattgacc cgaaaaaaat
    27661 tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga
    27721 agatgtggag aaaaaaatcc gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt
    27781 aagccgtgaa gagttcgacg tccaaacgca agtcctgtta cggactcgtg agaaactggc
    27841 gctgcttgaa caaagaatca gcgaactgga aaatcgcagt actgagatca aaaagcagcc
    27901 agatccagag actctccccc caacgctgta aacatttgtc tttaatgcac tgtaaattat
    27961 gaattaatcc tctaatttac agtgttttct attacattat cgcttaatcc cattcagagt
    28021 atcatcacaa aaatgaatga ttgcaattga aaatataatt gttatgtatt ctttaagttt
    28081 tacttaatcg at
//
LOCUS       DADLXX010000040        27741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLXX010000040 DADLXX010000000
VERSION     DADLXX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.040"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(136..211)
                     /locus_tag="JNX27_001743"
     tRNA            complement(136..211)
                     /locus_tag="JNX27_001743"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Lys,seq:ttt)
     gene            complement(376..1167)
                     /gene="cpoB"
                     /locus_tag="JNX27_001744"
     CDS             complement(376..1167)
                     /gene="cpoB"
                     /locus_tag="JNX27_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7882983.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAHAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1177..1698)
                     /gene="pal"
                     /locus_tag="JNX27_001745"
     CDS             complement(1177..1698)
                     /gene="pal"
                     /locus_tag="JNX27_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7882984.1"
                     /translation="MQLNKVLKGLMIALSVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1733..3025)
                     /gene="tolB"
                     /locus_tag="JNX27_001746"
     CDS             complement(1733..3025)
                     /gene="tolB"
                     /locus_tag="JNX27_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7882985.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3158..4399)
                     /gene="tolA"
                     /locus_tag="JNX27_001747"
     CDS             complement(3158..4399)
                     /gene="tolA"
                     /locus_tag="JNX27_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7882986.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4464..4892)
                     /gene="tolR"
                     /locus_tag="JNX27_001748"
     CDS             complement(4464..4892)
                     /gene="tolR"
                     /locus_tag="JNX27_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7882987.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4896..5588)
                     /gene="tolQ"
                     /locus_tag="JNX27_001749"
     CDS             complement(4896..5588)
                     /gene="tolQ"
                     /locus_tag="JNX27_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7882988.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5585..5989)
                     /gene="ybgC"
                     /locus_tag="JNX27_001750"
     CDS             complement(5585..5989)
                     /gene="ybgC"
                     /locus_tag="JNX27_001750"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7882989.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6139..6432)
                     /gene="ybgE"
                     /locus_tag="JNX27_001751"
     CDS             complement(6139..6432)
                     /gene="ybgE"
                     /locus_tag="JNX27_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7882990.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6432..6545)
                     /gene="cydX"
                     /locus_tag="JNX27_001752"
     CDS             complement(6432..6545)
                     /gene="cydX"
                     /locus_tag="JNX27_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7882991.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6560..7699)
                     /gene="cydB"
                     /locus_tag="JNX27_001753"
     CDS             complement(6560..7699)
                     /gene="cydB"
                     /locus_tag="JNX27_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7882992.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7715..9283)
                     /gene="cydA"
                     /locus_tag="JNX27_001754"
     CDS             complement(7715..9283)
                     /gene="cydA"
                     /locus_tag="JNX27_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7882993.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10132..12810)
                     /locus_tag="JNX27_001755"
     CDS             complement(10132..12810)
                     /locus_tag="JNX27_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY7882994.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLEPLATLAWTLGFE
                     YHHGLLEKIWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIAD
                     NMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPG
                     LIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENA
                     FWQIALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQ
                     CEIIHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLD
                     NQADDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNML
                     NYAALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGI
                     KMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKA
                     GFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISC
                     SETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12828..14804)
                     /gene="mngA"
                     /locus_tag="JNX27_001756"
     CDS             complement(12828..14804)
                     /gene="mngA"
                     /locus_tag="JNX27_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY7882995.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWESVDGPEAVDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNALSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMQ"
     gene            14914..15636
                     /gene="mngR"
                     /locus_tag="JNX27_001757"
     CDS             14914..15636
                     /gene="mngR"
                     /locus_tag="JNX27_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7882996.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15740..16609)
                     /gene="sucD"
                     /locus_tag="JNX27_001758"
     CDS             complement(15740..16609)
                     /gene="sucD"
                     /locus_tag="JNX27_001758"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7882997.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16609..17775)
                     /gene="sucC"
                     /locus_tag="JNX27_001759"
     CDS             complement(16609..17775)
                     /gene="sucC"
                     /locus_tag="JNX27_001759"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7882998.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17869..19086)
                     /gene="odhB"
                     /locus_tag="JNX27_001760"
     CDS             complement(17869..19086)
                     /gene="odhB"
                     /locus_tag="JNX27_001760"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7882999.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19101..21902)
                     /gene="sucA"
                     /locus_tag="JNX27_001761"
     CDS             complement(19101..21902)
                     /gene="sucA"
                     /locus_tag="JNX27_001761"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7883000.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22203..22919)
                     /locus_tag="JNX27_001762"
     CDS             complement(22203..22919)
                     /locus_tag="JNX27_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7883001.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22935..24701)
                     /gene="sdhA"
                     /locus_tag="JNX27_001763"
     CDS             complement(22935..24701)
                     /gene="sdhA"
                     /locus_tag="JNX27_001763"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7883002.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24701..25048)
                     /gene="sdhD"
                     /locus_tag="JNX27_001764"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24701..25048)
                     /gene="sdhD"
                     /locus_tag="JNX27_001764"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7883003.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25042..25446)
                     /gene="sdhC"
                     /locus_tag="JNX27_001765"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25042..25446)
                     /gene="sdhC"
                     /locus_tag="JNX27_001765"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7883004.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25827..25934
                     /locus_tag="JNX27_001766"
     CDS             25827..25934
                     /locus_tag="JNX27_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883005.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26140..27423
                     /gene="gltA"
                     /locus_tag="JNX27_001767"
     CDS             26140..27423
                     /gene="gltA"
                     /locus_tag="JNX27_001767"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7883006.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         6721 a         7485 c         6737 g         6798 t
ORIGIN      
        1 acactacgct ggatactaca actaactatg ttagtggtgg gtcgtgcagg atgactcgac
       61 ttcgtctcgc ccttcggcgc cgttgctgat gcaacgttat ccttcacatt ttactaccgc
      121 ttccacctta gcgattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
      181 tcaactgctc taccaactga gctaacgacc cactttttcg ttgctttcgg tttgtttgat
      241 atcccgtggc aacggcggca tatattactg atttcagact tgagcgcaac aaaaatttcg
      301 atgcagatca ctcaactgct tatgattcgc acgacacgac cagaaataat gcgacttctg
      361 gtcgtgtgtt atgcattaca tcgcgttcag acgtttttgt gcctgtttag cgccatcagt
      421 accagggtat ttactgataa cctgctggta tacggctttc gcttttgcgg tgtcaccttt
      481 gtcctgcatg atgacgccga ctttaaacat cgcatcagca gcctttggtg acttcggata
      541 gtttttcact accgaagcaa aatagtacgc cgcatcatct tttttaccct tgttgtagtt
      601 taactgaccc agccaataat tggcgtttgg caggtaagtt gaatcagggt aatttttgat
      661 gaaattctga aatgccacca ttgcgtcatc ctggcgggat ttatcctgca ccagcgcaat
      721 agctgcattg taatccgtgt ttgcatcgcc gcttttcacc ggcgcgccag cattcgcagt
      781 accagcatca gctgtcggcg tcgttgatgc cgccgcaccg ctttgatcgc tgctggttga
      841 ttgcgccgct gcaccaccgc tgctgaggct gtcgatctgc aacaggatct gcttttgccg
      901 ctccacgacc tgattcagtt gatactgatt ttcctgaatc tgaccacgca gggaatcaat
      961 atcggattga ttatcagaaa gttgttgctg gagttgggtt aaaagctggc tgtgagcatt
     1021 agaaatacgc tcaagttgag tgacgcggtc ttcgaccgag cctgagccga cactactgat
     1081 tggtgcatga gcaaaagcgg cccagggggc cgctatacca accagtaacg acagactcaa
     1141 tagttgatgt ctgaagttac tgctcatgca attctcttag taaaccagta ccgcacgacg
     1201 gtttttggag tatgctgctt cgtcatgacc cagtactgca ggtttttctt taccgtaaga
     1261 aacgatggag atctggtctg cagaaacgcc tttaccctgc aggtacatct taacggcgtt
     1321 cgcacgacgt tcacccaggg agatgttgta ttccggagta ccacgttcgt ccgcgtgacc
     1381 ttctacggtg actttgtaag acgggttgct acgcaggaag tttgcatgtg catccagcat
     1441 ttgagcgaag tcagaacgga tatcgtactt gtccagatcg aagtaaacga tgttgttctg
     1501 ctgcagctgt tgcatttgca gacgagcctg ctcttcggaa gacatgttgc cgttgccgcc
     1561 gttcgcatcc ataccagtgc cagcacccag catgccttcg ctgccgtcat tgctggcgtt
     1621 cttgttggaa gaacatgccg caattgccat aacagacaga gcaatcatca gccctttcag
     1681 cactttgttc agttgcattt caatgattcc tttactattc aattaattat tatcacagat
     1741 acggcgacca ggcagggaat ttgacctgtc catcagttgc cggaagacgc gctttgaaac
     1801 gcccatctgt agaaaccaaa ttcagcacgg atcccatccc ctgagaagag ctgtagatta
     1861 ccatagtgcc gttaggtgcc agacttggcg tttcatccag gaacgtggac gacagaactt
     1921 gtacgcctcc cgttgccaga tcttgtttgg caatgtgctg ctgcccacca ttggagctga
     1981 ccattaccat aaatttaccg tcgctgctga catccgcatc ctggttctgc gaaccttccc
     2041 aggtaatacg ttgtggcgca ccgccgttga tattcacttt ataaacctgc ggacgaccgg
     2101 cctggtcaga agtaaatgcc aggttctggc tatccgggaa ccaggtcggt tcggtattgt
     2161 tactgcgacc atcagtcacc tggcggatct gaccagaagc caaatccatt acgtacaggt
     2221 tcagactacc ggttttcgac aaggcgaatg ccagtttgct gccgtctggc gagaatgcag
     2281 gtgcaccgtt gtgacgcggg aatgaagcca cctgacgtac agcgccattt gccagcgtct
     2341 gaataaccag cgcggaacga ccgctttcga aggtcacata agccagttta gaaccgtctg
     2401 gtgaccacgc cggagacatc agcggctgcg gtgaacggtg aacgacaaac tggttgtaac
     2461 cgtcatagtc agatacgcgc agttcatacg ggaactgacc gccgttggtc tgaacaacgt
     2521 aggcaatacg ggtacggaac gcacctttaa tgccggtcag tttttcaaac acttcatcac
     2581 tggcggtatg accagcataa cgcagccact gcttgttcac tttgtacgag ttctgagcaa
     2641 gtacagtacc cggtgcgccg ccagtgtcaa caagttgata agcaacattg taagaaccat
     2701 ccggattcgg agtgacctga ccgacaacta cagcgtcaat gcccagtgcg gaccatgcag
     2761 ctggttgtac ttcctgcgca ctacccggct gctgtggcag acgagcgcga tctaacggat
     2821 taaatttacc gctgttacgc aagtcagcag caacgatgcc gccaatatct tcaggtgccg
     2881 caccaggccc cgcccactgg aaaggaacaa caccaatagg acgaccggaa tctacaccgc
     2941 tgtcgatcac aatgcggact tcagcatgca gaactgatgc ccacagtatg agaaaaccaa
     3001 atgctactcg taatgcctgc ttcatcatat ctcccttatc tggaccgatg gcccacgata
     3061 atttagcaga atgttaacaa actaaaatac acaaaactac cagaaccccg tggcaaccgg
     3121 tgcctgatgt tgaccgtccg aacagtcaac atcgcgatta cggtttgaag tccaatggcg
     3181 cgtttttgaa cacttcatat actgcctggc ttggtggttt cgggatcttc gcaagtttag
     3241 ctgctgccaa cgcagcctga caaagtgcgg gatcgccacc ttcaggtttg atatccagta
     3301 acataccatc gggtgccagt tttatgcgca gcgtacaggt tttgcctgca taggacgatg
     3361 cgtcatagaa cttactttcg atagcagatt taatctgccc ggcatagtta ttgatatcgg
     3421 cccctgatgc gccattgttt ttagtattac cactcccggc aggcgaagca ttgttccctt
     3481 tcgcccctcc ccccgttttc ggtgcattct taccagagct tagctcaccg aaaatatcat
     3541 ctgcctctgc ggcagctttt gctgcagcgg ctttttcagc tgctgctttt gccgctgccg
     3601 cttttttgtc tgcggcggct ttttctgccg ctgctttctt atcagctgct gccttatcag
     3661 cagccgcttt ttctgctgct tttttgtcgg ctgcagcttt ctctgctgcc gctttcttat
     3721 cagctgcagc cttttcagca gccgctttct tctcagcttc tgccttggct ttttcagcag
     3781 cttcagttgc cgctttcttt cttgcttcag ctgcagctgc ttctgccgct tccgctttct
     3841 tcttcagtgc cgcagcggct acctcggctt ttttctgcgc ttcggctgcg gctttggcgg
     3901 cttctgcttc tgcttttttc ttcgcgtctg cagccgcttt cttcgcagct tcttccgcag
     3961 ctttagcatc tgccgccgct ttcgctgccg ccacttcagc ttgcttctgc tttaactcgg
     4021 cctgttttgc ggcttcttca gcctgctttt tctgctcctg agccgctaac cgctctttct
     4081 caagttgctt caggcgttcc tgttcagccg cttgtttctc acgcagttct tcagcagcct
     4141 gctgttcctt catcttgcgc tgttcatcag aacgcttcgc gcttgattcc tggctttgca
     4201 tgcgtttgta ctgctcaact accgcacctg aatcaaccat gacagcgtcg atggacgaac
     4261 caccgccgcc tccggctgaa gcttctatat tctcatcgaa cgaactccag atcagcgccg
     4321 caaataagat gacatgcagc actgctgaaa taattatcgc ccgcttgagc ttgtcgtttt
     4381 gttcggttgc ctttgacact ctcggtttcc aaaaactgtt cgcctgttac ccgctctctt
     4441 tcaagcaagg gaaacgcaga tgtttagata ggctgcgtca ttaaaccaac cgatttcaca
     4501 cccgcactat gtaacaagtt cagtgcttta attatttcat cgtaaggcac atcttttgcg
     4561 ccaccgatca gaaagaccgt tttcgggttg gccttgaaac ggctggacac ttccgccacc
     4621 acctgctctg gtggtaaacg ctccaggcga tctttctcaa ccaccacggt gtactgacca
     4681 ataccagaca cttcaacaat cactggcgga ttatcgttac tgctcaccgc ctgtgattca
     4741 gtagcgtctg gcagatcgac ctccacgctc tgggtgatga tgggcgctgt cgccataaag
     4801 atcagcaaca gcaccagcag tacgtccagc aacggtacaa tgttgatttc ggacttgaga
     4861 tcgcgacgac ctcgtccacg cgctctggcc atggcttacc ccttgttgct ctcgctaacg
     4921 gtaaacgcct ggcggtgcag aatcgcggta aactcttcca taaagttgtc gtaattcagt
     4981 tccagtttgt ttacgcgctg gttgagacgg ttataagcca ttaccgccgg aattgcggcg
     5041 aacagaccaa ttgccgtcgc aatcaacgct tctgcgatac cgggcgcaac catttgcagt
     5101 gttgcttgtt ttaccgcccc gagggcgata aaggcgtgca tgatccccca gaccgtacca
     5161 aacagaccaa tatacgggct gatggagcca accgtaccga ggaacggaat gtgcgtttcc
     5221 agattttcaa gttcacggtt catggagata cgcatagcac gcgacgcccc ttccactacg
     5281 gcttccggcg catggctatt ggcacgatgc aggcgcacaa actctttgaa cccgctgtaa
     5341 aagatttgtt ccgaaccagt cagattatcc cgtttcccct gactctcttg atagaggcga
     5401 gagagttcga ttccagacca gaatttatct tcaaacgctt cggcttcacg cgccgctgcg
     5461 ttaagaatac gggttcgctg gataataatg gcccaggatg cgattgaaaa accaatcaaa
     5521 atcaacatga taagtttaac cagaaggcta gccttcagga acaaatcaag gatattcatg
     5581 tcagtcactg cttaaactcc gcgacaatag acttgggaag cgcacgaggc ttcattttga
     5641 gtgggtcaac gcaaacaacc agaacctctg cttcattcag caaagtattc tcggcgttga
     5701 caatatgttg cgtgaaaacc aaagaggtgc cacgcattga tgttatttca gtctgtattt
     5761 cgagcatatc gtcgagccgc gcaggtgcgt aatattccac cgtcatttta cgtaccacaa
     5821 aggcaacgcg ttcagccatc agcgcctgct gactgaagtg atgatgacgc agcatctctg
     5881 tgcgtgctct ttcataaaaa gcgacgtaac tggcgtggta caccacacca ccggcatcgg
     5941 tatcttcata gtagacgcga accggccatc gaaacagcgt tgtattcact ttacatcccg
     6001 gtaatgcaac aaaagttaga gcttttaaac gctgctacta tacgcgcgcg attgaggttt
     6061 gggaatgagg gcaagttaag ggagcgtaaa aatttatggg cctggagcgg cccataattt
     6121 tttaaagaga attctgattt aaaagaagaa gaaaatcagc ccgacaatga gaacaatatc
     6181 ggcaagcaat gggcaaaaaa tcccttgcca aagaaccttc tgcggacgaa agcccacgcc
     6241 gtgaatcaca ccagcacaga cggcccacat cagcaataaa ccatgccaga tttccagttc
     6301 actggtcttc gcggcaaaac gagatgggtc ccaaaacata catcctgcta acagaagcgc
     6361 catcacgaag gaaagcgccc gcagggggcg cttgtccatt accgcataca aggtcgcgat
     6421 aatcttactc atcagatgtc ttcttgaccg gctttgcctg attcgacgtg ttcaagcgcc
     6481 agcgcggtga ttaccccaaa cgaacaggca agaagcgttc ccagaatcca tgcgaaatac
     6541 cacattttta gctccttact tagtacagag agtgggtgtt acgttcaata tcttctttgg
     6601 tgatacgacc gaacattttc cagtaacacc aggcggtgta gagcagaatg atcggtacca
     6661 gaaccaccgc aacccaggtc atgacgttaa gcgtcagctg gctggaagtt gcatcccaca
     6721 ttgtcagact tgcgttcatc atggtgctgg acggcatcac aaacgggaac attgcgatac
     6781 cggctgtcag gatgatgcag gccagcgtca gggaggagaa cacaaacgcc cacgcggctt
     6841 tatccatacg tgcagtcagg atggtcagca gcggcagaac cacacccagt gccggaatag
     6901 cccacagaat tggcgtgttg ttgaagttaa ccagccatgc gccagcttca cgaaccactt
     6961 ctttattcag tgggttagag gctgcgtaat ggtccattgt cgatttcacg acataaccat
     7021 cgataccgta catcacccat acgccagcca gtgcgaaaca gaccagtgtc accagcgcag
     7081 ccacctgagc cgttgcacgg gtacgcaggt gcagttcgcc cccggtacgc atttgcagat
     7141 aggttgcgcc ctgagtaatg atcatcccta cgctcaccac gcctgccagc aggccgaacg
     7201 ggttaagcaa ctggaagaag ttaccggtgt agtacagacg cagatattca tcaacgttga
     7261 acggtacgcc ctgcaacagg ttaccgaacg ctacaccaat taccagcggc ggaacgaagc
     7321 taccaatgaa gatgccccag tcccacatgt tacgccagcg ggtttcttca atcttggagc
     7381 ggtagtcaaa accgaccgga cggaagaaca aagacgccag cacgaggatc atcgccacat
     7441 agaagccgga gaacgcagcg gcatagacca tcggccaggc agcaaagagt gcgccgcccg
     7501 cggtgatcag ccaaacctgg ttaccgtccc agtgtggtgc aatggagtta atcataattc
     7561 gacgctcggt gtcgttacga ccgaggaaac gggtgagcat gcccaccccc atgtcgaaac
     7621 cgtcagtgac tgcaaaacca atcagcagaa cgccaaccag cagccaccag ataaaacgca
     7681 atacttcata atcgatcatt tgacgactcc tgtcttagcg tgccggctga gtagtcgtgg
     7741 aagactgctc aaagtgatag cgaccggttt tcaggctgct tgggccgagg cgtgcaaact
     7801 tgaacattaa gaacaattct gccaccagga acagggtata caggccgcaa atcagcacca
     7861 ttgagaagat gagatcgcct gcggtcagtg acgaattcgc cacagctgtc ggcagcactt
     7921 caccgatagc ccacggttgg cggccatatt cagccacgaa ccagcccgct tctacagcaa
     7981 tccacggcag cggaataccg tacagcgcgg cgcgcagaag ccattttttc tcgccaatgc
     8041 ggttgcggat gacactccag aaagagagcg cgatgattgc cagcagcagg aagccacacg
     8101 ccaccatgat acggaacgca aagtacagcg gcgctacacg cgggatggag tctttggttg
     8161 cctgttgaat ctgcgcttca gtcgcatcag ccacgtttgg cgtatagcgt ttcagcagca
     8221 gaccgtaacc gaggtctttc ttcatgctat tgaactggtc acgaaccgcc tggtcggtag
     8281 aaccagaacg cagttgttcg agcagagagt acgccttcat cccgttacga atgcgttctt
     8341 catgctgcac catcagctct ttcaggccga taaccggggt atccacggaa cgcgttgcaa
     8401 tgatgcccag tgcgtaaggg atctgaatcg caaatttgtt cgtctcctct tcctgatcag
     8461 gaatgccgaa caaagtaaag gcagcaggcg caggttgcgt ttcccactcg gcttcaatag
     8521 cagccagttt ggttttctgc acgtcgccca tttcgtagcc ggattcatca cccagaacaa
     8581 taacagacag aacagcagcc ataccgaagc tggcagcgat agcaaaggag cgtttagcga
     8641 aggcgaagtc acgacctttc agcatatacc atgcgctgat accgaggatg aacatcgcgc
     8701 cagtcacata accagacgct acagtgtgaa cgaatttcac ctgagcaacc gggttaagca
     8761 ccagctcgga gaagctcacc atctccatac gcatagtttc aaagttgaaa tcggacgcga
     8821 ttgggttttg catccagccg ttcgcaatca gaatccacag tgcggacagg tttgaaccga
     8881 gcgccaccag ccaggtgaca cacatatgct gaactttacc cagacgatcc caaccgaaga
     8941 agaacagacc tacaaaggtg gattcgagga agaaggccat cagaccttcg attgccagcg
     9001 gcgcaccgaa gatatcccct acatagtggg aatagtaaga ccagttagtc ccgaactgga
     9061 actccatggt cagaccggta gccacaccca gagcgaagtt gataccaaac aacttgcccc
     9121 agaacttggt catatcttta taaatctgtt tgccggagag gacgtagacc gtttccataa
     9181 tggccaggag gaacgccata ccgagcgtca gtggcacaaa aaggaagtgg tacatcgcgg
     9241 tcaaggcaaa ctgtaagcgc gacagttcga ctatatctaa catcatgact ccttgctcat
     9301 cgcatgaaga ctccgagagt gaaccccgtt agaaagggtc acacgcatgc cccaatagaa
     9361 atttgtttga acactttcat cataccatgt gaatgcacac aaaaacggcg taaagaaagg
     9421 ttgcaaacat gttaataaaa actcaaattg atcccacgta tatattacgc cgcaaaatcc
     9481 ttacaataaa caggtttttg ttgaagcaaa tttgcatttt tcgacggtga tcaatttata
     9541 gctaaattgc cgcctttcag ccaatttgat cgagaacaat ttatctcttt ttgatgccca
     9601 tttccaagac ttatacattg ataaatatca atttctacaa attatgttaa tcatgtttca
     9661 acaaggtaac tttagtgaca tttatgttta aaatgtgtga attataagac tgaaattagt
     9721 ttcggttggc aattaaagat aaaaagagac gaagcaaaaa cagcacggtt gaccttaatt
     9781 ctttttttgg tcactggcta atacgacgca gagtttcagg attattaata aaggcataaa
     9841 tctcctcccc gccgatatta acatttaatt aacttataat tatattataa ttaactcacc
     9901 cggggttatt attttgttaa aaataaagcc acatatacgt agcttgagag tgaagataaa
     9961 cgcaaaatta cctgatgcgc tacgcttgcc agtgtctaca tcatctctgc aatatattga
    10021 atttgcctgc ttttgtagac cgaataaagc gttcatgccg tatccggcat gaacaaagcg
    10081 ccctttgtca acaatctggc cgcgcatatg tggcttcggt ttgattgcta ttcaggcaag
    10141 ccggtaactg aacgtccgtg actggccggg taaaaaaggc cccgatagat ttgaaccttg
    10201 attttcctcg gtggtaatgt gttcatccat catcgtttct gagcaagaaa tcacctcgcg
    10261 actgaaagca acagtcgcat cacaggttgc tgattcagcc ggattaaaca gccgtaaaat
    10321 cacttcttgt cggtcttcag ctttcttaag tgcgcttatc aggcatccca ctgggggcat
    10381 tttcaacaaa ctataacttt ccggcacgtt gaatccggct ttgttgagct tcatcgcatc
    10441 ccatgggatt ttgttgtaac actgtactgg agtcagccat gctcgcgcct gctgagctac
    10501 accagcggcg gttggcgtac cggtataact caataaactt aggcgacaag aaagcagacc
    10561 acgtagttgt gagtccggga ctggcatttt aattcccgaa ggccgcctag gccttaaaag
    10621 cagatcttct ttccccagta agcccacgcc gcgcagcaac gtaatggcaa aggttttctt
    10681 ctcttcaccg atgacttcaa attcacgtag cccttcgcta aagacagcca tgccgttacg
    10741 cccttcctgt aaggctgcat agttgagcat attccatacc ggaaccggcg cttctttcca
    10801 gccttcttgc tgccagttgt tcattgcact gtcgttcacg gggcgcgtta gcgaaccaaa
    10861 ctgggtatct gccagaacac tatcggtgtt aaaagatgta gggatcagga cgcgaaggcg
    10921 atgatcgtca gcctggttat caaggttgat atccacatca atgcgcctgt tgttatgact
    10981 aagagtgaca accaactcga cacctactct gccagtggat tgtcttgcgc tgcgttgttg
    11041 cagattacgc ggcaccgcca tttcatagcg gataacagcc ctgctctgcc aggcctcatg
    11101 aataatctcg cattgcggtt tcgcattcgc cgaagtaatt acccactctt cttttgcggg
    11161 tgaatagtca tattcatcac catcatcaga gctttcttca atttgcaaca cccggtcata
    11221 gcgcacaccg ctatctttat ctaccagttg tagcgttcca tcctcattga gcgcaatttg
    11281 ccagaaagca ttttccagta tcccttcagc gtcacttttt gccgcaatca cgttgccagg
    11341 ctgattcgct tcgatataaa gcgtgcgata gcccatagaa gggacaatct ggttgatctg
    11401 tatatcaaac tccataaagg gatcgtaatt accgtaatga actatttgcc gatcgattag
    11461 gcctggatcg atctcacggg catggcgaat aaaatacggt acaggctgac cgcgatcgtc
    11521 tcgtagatta aactggctgg cgcgcagccg cacagtggtg ttgatcactt cttcccgcgg
    11581 ccagggcatc aggttaaaca ggacgagttt gtcggcgtcg ctctgcggca tgttgtcggc
    11641 gattttacgc atatagaaac gcagcagatt atccgccatg tcttcagcca gttcgaagcg
    11701 ggcgacgatt tcgcgatgaa ctttgtcact acagcagcag ccgatgctgt cgtgggcatg
    11761 attttttaag atctctttcc atattttctc cagcaagccg tggtggtatt caaaacccaa
    11821 cgtccaggcc agcgttgcca gcggttccag cggttccagc ggttccagcg gttccagcgg
    11881 ttccagcggt tccagcagat taacaatctt attttcaata cgtgcgtggg caactttgat
    11941 atccatgcgc gtggaaccga tggtgcgatg cacgcgcata tatttgccat caataaattc
    12001 ccctttcagg gttgccagat tatctcgctg cgcttcgatc ttctcaaata cctcttcaaa
    12061 gcggctcatc acaaactttc gttgaggata aatttcgcgc agtttctcca tcacttcgaa
    12121 gatattttgc tgcaatggca tctgatcatg cccattcggc agcaaaatct ctttggttac
    12181 agacgctttt tccagcacgt caaaataact gtcgaggcgt ttgcggaatc cgttttcgtc
    12241 cgcaggtaag tacttaccga tggcataacc caacggcatc acctgcgccg tcacttcgct
    12301 accgtcactg ctttgccaca aaaactcggt tttatccgta ccgtggcgct cagaacatcc
    12361 acgccagaac atggtgcagg taatgccaaa tccattgtag atatgcggca gttgcccgga
    12421 catgccaaag gaatcgggta aataacctat tttcatcggc tcgccaaacg cgaggcagtc
    12481 acgcattccg tacatcagat tacggacgat ggattccgca gaaacaatcg tggtatcggt
    12541 ctgggtatac caggggccga taatcaactt gccggcttct accagtttct tcacgcggtc
    12601 tttgttttcc ggtttcaccg cgaaataatc ttcgaggatc gccgtttgcc cgtcgagtac
    12661 gtaatatttg tattcgttgt cctgttccag tcggcacagg atctcttcca tattattgac
    12721 cagcagaatg cgtgactctt cggtggtgaa ataccactcc cgatcccagt gcatatgcgg
    12781 ggtgatgtga acgcgagata ccgctttcat cgtcgtttcc tttttgttta ttgcattacg
    12841 ccatcagtca gatagttgcc atgcttaacc gcgtgacgcc gccagatcag gagaattgca
    12901 gtcgagattg cagcccccac caatgccgca ccaaaccagc caattgccgc cataacaccg
    12961 cccgcgccat tatcatgaag taaaaagagc gagaaaatgc cagcaccggg tgtcgaaagg
    13021 ccgatattca tcgcaccgac aatagcgccc gttaccatag agcccagcac aaacgaacca
    13081 ataacccgca gcggatcttc aatcgccatc gggatcgccc cttcggtaat acctgccagc
    13141 cctaacagcc aggtggattt cccggtctca atttcaaact ctttaaacag gcgcggtgcg
    13201 agcatcgtgg aagcggttac ggtaaatgcc gaaaccattt tgacggaggc gaaaatggca
    13261 tacgggccgt aaacgccgtt cgccattgcg cccaggcaga atgcataagc ggctttattc
    13321 actggccctc caaggtcaaa ggaacacata aaaccgagaa tggcacccag caacagcgcg
    13381 ttacttcctg acagagcgtt cagccaggcg gtaagcgagt tattgatcca ggcgacaggt
    13441 tcccccacca caaacagcat cagactgccc gctcccaatg taccgagcac caggtagaga
    13501 taaaaagtca ggaagccatt gaatttactg ctaagacgca agtgattttt catccagcgt
    13561 atcaagtaac cggctatcaa tccgccaacg accgcgccga gaaacccgga gccgatcatg
    13621 ttggcggcaa gtccagccgc aaagcctggc gctaacgccg gtttatctgc cagcgaatag
    13681 gcggtatagg ctgccagcac cggtaccatc aaaattccga gcagcccgcc gcccagtttg
    13741 cggtacatcc acagccagga gttttcttca ttaaacagat cttgtagccc gaagatttgc
    13801 gacagtaata ccgcgaccgc cagcaccgtc ccccccgcga caatcaacgg tacggcaaaa
    13861 gagatcccgc tcaacagtgc ctgtttcagc tccgttttga cgcttttcac cggttgcgta
    13921 tcttgctgta cggtacgcgt ctcatcgcca cgctcgaggg taagcgattg ttggatcaac
    13981 gcttctgcat ggcgaatcgg ctcggcaaca ggcacagaaa gcgcaggaat gccgttaaaa
    14041 cgctcactct ccttgatggc gacttcagcc gcaaaaatac aggcagttgc actattgagt
    14101 tgatccgccg ttaaacgccc ttcaataccg ttagcacctt gcttttcaac gtagacattc
    14161 acgccgagtt tgcgtccggc tttttccaga tactccgcag ccatgtaggt gtgagcaata
    14221 cccgccgggc aggccgttac gcaaacgata gttggcgcgt tggaaaaaga ggcagaaggt
    14281 tgcgtgcctc ccttgtcatc cagcgccgag agcaattcat caggcgtcgt cgccgactgt
    14341 atacgcgccc gaatctcatc atccgccagg cgcgtggtca gcgctgtcag cagttgcatg
    14401 tgcgtagtac ccgcttcatt ggggggaatc gccagcaaca ccactaaatc aaccgcttcc
    14461 gggccatcca cgctttccca ctgaagcggc tcgctgagtg tggcgaccgc aaacgccgct
    14521 tcttttaccg cagcagtttt gccatgcggc acagccaacc cttcacctaa ggcggtcggg
    14581 ccaaggcttt cacggcgata cacttctttc agaaattgct cagtactgga aattttcccc
    14641 agagcagcaa gacgttgagt caacgcgtga atggcctctt cacggctggt aaagcgcgca
    14701 ttcagacaca acgcatcgcg gtgggtcaga gtcgtcaggt tcatcatgat cctcacttga
    14761 tcgtttttat agtcgcgcca gtgtgcccga taaaacaata ccattgcgta atcaatttca
    14821 cttgattgaa attatttgca ttgtatttgt attatttaca ggatgaattt atcgggcata
    14881 atggctaaat tcgtattaat gagatgctaa ataatgggac acaaaccctt ataccgacag
    14941 attgctgatc gcattcgtga gcaaattgcg cgtggcgagc ttaaacctgg cgatgcgtta
    15001 cctaccgaat cggcattaca aacagagttt ggcgtcagcc gggttacggt gcgtcaggct
    15061 ttgcgccagt tagtcgagca acagatcctc gaaagcattc agggcagcgg gacttacgtc
    15121 aaagaagagc gggtcaatta cgatattttt cagttaacca gttttgatga aaaactgtcg
    15181 gaccgtcacg tcgatacaca cagtgaagtt ctgatatttg aagtgattcc ggctgacgat
    15241 tttcttcagc aacagctaca aatcaccgtg caggatcgtg tgtggcatgt gaagcgcgtg
    15301 cgctatcgca agcaaaagcc aatggcgctg gaagaaacct ggatgccgct tgcgttgttc
    15361 ccggatctca cctggcaggt catggaaaat tcgaaatatc actttattga agaagtgaag
    15421 aagatggtta tcgatcgtag cgaacaggaa atcattcctc tgatgccaac cgaagagatg
    15481 agccgtctgc ttaatatcag ccagacaaag ccgattcttg aaaaagtatc gcgtggatat
    15541 ttagttgatg gtcgggtgtt tgaatatagc cgcaacgctt ttaataccga tgactataaa
    15601 tttaccctga tagcgcagcg aaaatcatcg cgataagcac aaaaaaggcc atcatacgat
    15661 ggccaaccat gtcgaaaccg acagcccttc gccattcctg tagagggaag cggcgagggc
    15721 tatttcttat tacagatatt tatttcagaa cagttttcag tgcttcaccg atatccgcca
    15781 ggctgcgaac ggttttcacg cctgcggctt ccagagcagc gaatttctca tccgcagtcc
    15841 ctttcccacc ggcaatgatc gcacccgcgt ggcccatacg tttgcctttc ggcgcagtca
    15901 caccagcgat gtaacccaca actggcttgg taacatgctc tttgatgtac gctgccgctt
    15961 cttcttcagc gctaccgccg atctcaccga tcatcacgat cgcttcggtc tgcggatctt
    16021 tttcgaacat ttcgagaatg tcgataaagt tagagccagg gatcgggtca ccgccgatac
    16081 cgacacaggt cgactgaccg aaaccgtaat ccgtggtctg tttaaccgct tcataggtca
    16141 gtgtaccgga acgggaaacg atacccactt tacccggttt gtgaatgtga cccggctgga
    16201 taccgatttt gcattcaccc ggggtgataa cgcctgggca gttcgggccg atcatacgaa
    16261 cgcctgcttc gtccagtttc actttcacgg tcagcatatc cagcgtcggg atgccttcag
    16321 tgatggtgat aatcagtttg atgcctgcat cgatagcttc cagaatggag tctttgcaga
    16381 acggtgctgg tacgtaaata acagacgcgg aagcgccagt agcagcaaca gcttcacgca
    16441 cggtgttgaa caccggcagg ccgaggtggg tagtgccgcc tttacctgga gttacgccac
    16501 caaccatttt agtgccgtat gcaatggcct gttctgagtg gaaagtcccc tggctaccgg
    16561 taaagccctg gcagataacc ttggtgtttt tatcaattaa aatggacatt atttcccctc
    16621 cactgcggca acaacctgct gagctgcatc cgtcagacct tttgctgcaa taatattcag
    16681 gccgctgtca gccagtttct tcgcgccgag ttcggcgttg ttaccttcca gacgtaccac
    16741 gaccggtacg ttaacaccca cttccgctac cgcgccgatg ataccgtcag cgatcaggtc
    16801 gcaacgaact ataccgccga agatgttaac cagaacggct ttcactttgt cgtcagagag
    16861 gatgattttg aacgcttcgg ttacacgttc tttggttgcg ccgccgccaa cgtccaggaa
    16921 gttagccggt tcgccgccgt gcagtttaac tatgtccatc gtacccatcg ccagacctgc
    16981 gccgttaacc atacaaccga tgttaccgtc cagcgcaacg tagttcagtt cccactgtgc
    17041 agcctgtgct tcacgcggat cttcctgcga ctggtcacgc atttcgcgca gatcaggctg
    17101 gcggaacagg gcgttgccat cagcgcccag tttgccgtcg aggcaaatca gatcgccctg
    17161 tttggtgatg accagcgggt tgatttcgat cagcgccagg tcgcgctcca ggaaaatggt
    17221 cgccaggccc atgaagattt tggtgaactg ctgaaccagt ttaccttcca gacccagttt
    17281 gaacgccagc tcgcgtccct gatacggcat cgggccagtc agcggatcaa gcgcaacttt
    17341 atggatcagg tgcggagttt cttccgccac tttttcgatt tccacgccgc cttcggtgga
    17401 ggccataaag accacgcgac gggaactacg gtctacaacg gcaccgagat acagctcttt
    17461 agcgatatcg gtcgccgctt caaccagaat ctggttaacc ggttggccat tggcatctgt
    17521 ttgatacgtt accagacgct tgcccagcca gttttctgca aaagcacgga tgtcttcttt
    17581 gctgtttaca actttcacac cgcccgcttt accgcggcca ccagcgtgaa cctgacattt
    17641 cactacccac ggaccggcac cgatttttga agcggcttct tctgcttcgc gcggagtagt
    17701 acaggcataa cccaccggtg ctggtaagcc atagcgggca aacagttgtt ttgcctgata
    17761 ttcatgtaag ttcatgtgtt ctgtccatcc ttcagtaatc gttatctttt aaaccgtaga
    17821 ccgacaccct atccacgtcg gtctacagtg caggtgaagc ttaaactact acacgtccag
    17881 cagcagacgc gtcggctctt ccagcaactc tttgatcgtt accaggaagc ccacggattc
    17941 gcgaccatcg atcagacggt gatcgtagga cagcgccagg tacatcatcg gcaggatctc
    18001 aacctgacca ttcaccgcca tcggacgatc tttgatagcg tgcataccca gaattgcgct
    18061 ctgcggcggg ttgatgatcg gcgtagacat cagggaaccg aacacaccac cgttggtgat
    18121 ggtgaagtta ccaccggtca gatcttcaac cgtcagcttg ccatcacggc ctttgactgc
    18181 cagctctttg attttcttct cgatgtctgc catgccgagg gtatcgacat cacgcagaac
    18241 cggcgtcacc aggccgcgcg gcgtagaaac cgccatgctg acgtcgaaat agttgtggta
    18301 aaccacgtca tcgccgtcga tagacgcgtt cacttccggg taacgtttca gggcttcaac
    18361 caccgctttc acgtagaagg acataaagcc cagacggatg ccgtggcgtt tttcaaacgc
    18421 ttcaccgtac tgcttacgca gatccataat cggcttcatg ttgacttcgt tgaacgtggt
    18481 cagcatggcg gtggagtttt tcgcttccag cagacgctct gccacacgct tacgcaggcg
    18541 agtcatcggg acacgttttt cactacgtgc agccagagcc ggttgcgccg ccggagcagc
    18601 cgctgccgga gcagactctt tcgccggggc tttcgccaga tgtttttcca catcttcacg
    18661 agtcagacga ccacccacac cggtgccttt aatggcgctg gcgtcgagat tgtgttcagc
    18721 cagcaggcga cggatcgccg ggcttaacgc atcgttgttt tgctcttcca gagacgcctg
    18781 ctggcgttgc gccggagtgg acgctttctc ttcagatttg gcgctggttt ctttaccggc
    18841 gctgttgcct tcacgcaggc gaccaaggat ctgacgagac gttaccgttg taccttcatc
    18901 ttccagaacc gcatccagaa tgccgtctgc tgatgccggt acttccagta ccactttgtc
    18961 agtttcgatt tctaccagca cttcatcacg tacgactgcg tcgccgggtt ttttatgcca
    19021 ggttgcgacg gtggcatcgg ctacggattc aggcaggtca gggaccagaa tatctacgct
    19081 actcattgtg tatcctttat ttattcgacg ttcagcgcgt cattaaccag atcttgttgc
    19141 tgtttctggt gaacggacat ataccctacc gccggagagg cggaggccgg gcggcctgca
    19201 taacgcagag aagccccaaa cggaatcact tcacggaaat gatgctggct gcagtaccat
    19261 gcgccctggt tgagcggctc ttcctggcac cagacaaaat ccttgacgtg agcaaactgc
    19321 tgcaacactt cctgcatcgc tttatgcggg aacgggtaga gttgctcgat acgcacaatg
    19381 gcgacatcgt gttgattgtt cttacgacgc tgttccagca ggtcgtaata aaccttacca
    19441 gaacacatca ctacgcgctt cacgcccttc ggatcaagtt cgtcgatttc accgatggct
    19501 ggcaggaagg tgccgttcgc cagttcttcg aggctggaaa ccgccagcgg atgacgcagc
    19561 agggatttcg gcgacatcac gaccagcgga cgacgcatcc cgcgcagcgc ctgacgacgc
    19621 agcatgtggt aaacctgtgc cggggtagac ggtacgcaaa cctgcatgtt ttgctcagca
    19681 caaagttgca gataacgttc cagacgcgcg gaggagtgct ccggcccctg cccttcgtaa
    19741 ccgtgcggca gcaacatcac cagaccacac atccggcccc atttctgctc gccagaggag
    19801 atgaactggt cgataaccac ctgcgcaccg ttggcgaagt caccgaactg cgcttcccag
    19861 atggtcaggg tgcgtggttc tgcggtggca taaccatatt caaacgccag cacggcttct
    19921 tctgacagta cggagtccca gacacggaac gcgccctgac cgttatggat gtgttgcagc
    19981 ggcgtgtaag tggaaccgtt agactggttg tggatcaccg cgtggcggtg gaagaaggta
    20041 ccgcgaccgg agtcttcacc cgacaggcga accggaatgc cttcatcaac cagcgtggcg
    20101 taagcgaggt tttccgcacc gccccagtcg aacagtttct caccggctgc catcgcctgg
    20161 cgatcgccat aaatcttggc aacgcgagac tgcatttcaa ctgcttctgg taccgtactg
    20221 atacgtttag ccagttcctg caggcgcttc atctcaactt tgttcgggta ctcttcgtcc
    20281 cattcgtggt tgaggtacgg cgaccaggtg aaagagtgca tgttcatcgg acgccactct
    20341 gccactacgc aatcgccagc atccagcgca tcgcggtaca ggttaaccat ctcggtggca
    20401 tcttccagcg tcgccacttt ttcctgctcc agcttgtcag cgtagatttt gcgcggtgtc
    20461 ggatgttttt tgattttctg atacatcagt ggctgggttg cgctcggctc gtcggcttcg
    20521 ttgtggccgt gacggcggta gcataccagg tcgatgaaga catcacgttt aaaggtgtta
    20581 cggaaatcga gcgccagacg ggtcacaaag gcaacggctt ccggatcatc cgcgttaacg
    20641 tggaaaatcg gtgcctgaac catcttaccg atatcagtac agtacggtgt agaacgggca
    20701 tccagcgggt tagaggtggt gaaaccaacc tggttgttga taacgatacg taccgtaccg
    20761 ccaacttcat aaccacgcgc tttcgacatg ttcagagttt cctgaaccac gccctgcccg
    20821 gtcactgcgg cgtcaccgtg gatggtgatt ggcagcactt tgttgctgct cggctcatca
    20881 agtctgtcca gacgggcacg aacagagccg ataacgaccg ggcttacaat ctcaaggtga
    20941 gacgggttaa acgccagcgc caggtgcacc aggccgccat cggtctggaa gtcagacgag
    21001 aagcccatgt ggtatttcac gtcgcctgtg ccgaggtgtt ctttatgttt accggcgaac
    21061 tcgtcgaaca agtcttgcgg ttttttaccc agcacgttca ccagcacgtt cagacgacca
    21121 cggtgcgcca tcccgagaac cacttcgcgg gtgccgctgt tgccagcgtg gcggatcatc
    21181 tctttaagca tcgggattaa cgcgtcaccg ccttccagcg agaagcgttt tgcgccaggg
    21241 aattttgcgc cgaggtaacg ctcaaggcct tcagcggcgg tcagttcgct taagaagcgt
    21301 tttttctctt cgctattgaa agtcgcgcga ccagactcga tacgctgttg gatccagcgt
    21361 ttttcttcgg tgctggtgat gtgcatatac tcggcaccaa tcgggccgca gtaggtttgc
    21421 ttgagggcgg aaataagctc gccgagtttc atggtttctt tgccgctggc aaatgaaccg
    21481 acgttgaagg tttcctggaa gtctgcttcg gtcagatcgt ggaaagacgg atccagatcg
    21541 gccactttat cttgctgcca cagtcccagc ggatctagat tcgcatgctg gtgaccacgg
    21601 aagcggtatg cgttaatgag ctgcaggact ttaacctgct tcacattggt gtcagggtcg
    21661 gagatcgttg aagagtaacg tgaagcgtct ttcgccaggc ggcggaaata ttcacgcgtt
    21721 tgagagtgga attgatccgg tttgactccc gtaccaggta actgctggaa cgtcgaacgc
    21781 cagttagcgt caaccgagtc aggatcggtt aagaagtctt catagagctg ttctatccag
    21841 ctctggtttg cgccagagag gtaagaagag tccaaccagg ctttcaaagc gctgttctgc
    21901 atcgtgatcc cttaagcatc ttttttatgc ttacttcgcc gtggatacta ccacgcacag
    21961 tgatgtgcgt gccggggttc acttgcgagc tcttgtctac gtagtggctc gcgaaggaac
    22021 ctttaaaaac tgtcttagtg tcaggggcag tttttaaaga tttcctgcat ttctttgtta
    22081 cgcctgatgc gctgcgctta tcaggcctgc gggtaatgac ttgtaggccg gataaggcgt
    22141 tcacgccgca tccggcactg gttgcctgat gcaacgcttg cgcgtcttat caggcctaca
    22201 gtttacgcat tacgttgcaa caacatcgac ttgatatggc cgatggcgcg cgtcgggttc
    22261 agccccttcg gacatacact gacgcagttc atgatgctgt gacagcggaa tacgctgaat
    22321 gcatcgctca aaccgtcgag gcggctgtca gtctcggtat cacggctatc gatcaggaaa
    22381 cgatatgccg ctaacaagcc tgccgggccg ataaacttat cgggattcca ccagaaagac
    22441 ggacaagagg ttgaacaaca tgcgcagaga atacattcat acagcccgtc gagtttttcg
    22501 cgctgctctg gcatctgtaa atgctcgcga gctggcggat tttgtccatt attcaacagg
    22561 taaggcttaa ttttctcata ttgcgcatag aattgtccca tgtctaccac caaatcgcgg
    22621 atcaccggta aacctggcag cgggcgaatc acaatcttct tgcccggctg gttgagtgcc
    22681 gaaatcgggg taatacaggc cagaccattc ttgccgttca tgttcagacc gtcggaaccg
    22741 cacacacctt cacggcagga gcggcggaac gacaggctgg gatctttctc tttcagctgg
    22801 ataagcgcat ccagcagcat catgtcgcga ccttcttccg cttccagggt gtaatcctgc
    22861 atacgcggag catcatcaac atccgggtta tagcgataaa ttgaaaactc gagtctcatt
    22921 ttcctgtctc cgcattagta agtacgaatc ttcggcggga atgccgggcg cagtttcggt
    22981 tccatgttga cgcttcggcg cgtcatggat tccgactcag gcagatacag ggagtggcac
    23041 agccagtttt catcatcacg atccgggaag tcgaagcggc tatgtgcgcc acggctttcg
    23101 gtacggaagt tggcagaaac agccgttgca tacgccgttt ccatcaggtt atccagttcc
    23161 aggcactcaa cgcgctgggt gttgaactcg ctggaagtgt catccagacg ggcatttttc
    23221 agacgctcgc ggatcacttt caactgctca agccctttcg ccatcgcatc accttcacgg
    23281 aagaccgaga agttatgctg catacattct tgcagcgctt tacggatcgc caccggatct
    23341 tcaccgttac gattattgtt ccaccggttc aggcgatcca gagacgcttc aacatcagac
    23401 tcgctggcat cgcgcagtgc gccctgctcg gcgatagact cttgcagatg cagacctgcc
    23461 gcgcgaccaa agaccaccag gtccagcagc gagttgccgc ccagacggtt agcgccgtgt
    23521 accgatacac aagcgatttc accaacggca aacagtcccg gaacaaccac atcttcgcct
    23581 ttctcattca cagtcagcgc ctgaccggta actttggtcg gaataccgcc catcatgtag
    23641 tgacaggttg ggataaccgg aatcggctct ttcaccggat cgacgtgagc gaaggtacgg
    23701 gaaagctcca ggatacccgg cagacgggat tcgagaactt ctttacccag gtgatcgagt
    23761 ttcagtttcg cgtgtggccc ccacggacca tcacagccgc gaccttcacg gatttcaatc
    23821 atgatggaac gcgccaccac atcacggccc gccaggtctt tggcgttcgg cgcataacgc
    23881 tccataaagc gttcgccatg tttgttcagc agataaccgc cttcaccacg gcaaccttcg
    23941 gtgaccagta cgcccgcacc ggcaatgccg gtcgggtgga attgccacat ttccatatcc
    24001 tgcaccggta cgcctgcgcg gatagccatg ccgacaccgt cgccagtgtt aatgtgggcg
    24061 ttagtggtgg actgataaat acgccctgcc ccgccagtcg ccagcacggt agcgcgggct
    24121 ttgaaataaa ccacttcacc ggtttcgatg cacagtgcgg tacaacccac caccgcgcca
    24181 tcctggtttt tcaccagatc cagcgcatac cactcggaga aaatggtggt gtggtttttc
    24241 aggttctgct gataaagcgt gtgcaacagt gcgtgaccgg tacggtcagc agccgccgca
    24301 gtgcgtgccg cctgctcgcc gccgaagttt ttcgactgac cgccaaacgg acgttgatag
    24361 atacgaccat catcgagacg cgagaacggc aggcccatgt gttcgagttc cagaatcgct
    24421 tccggcccgg ttttacacat atattcaatc gcgtcctggt caccgatata gtccgaccct
    24481 tttacggtgt cgtacatatg ccattcccag ttatcttcat gggtattacc cagcgcaacg
    24541 gtaatgccgc cttgcgcaga aacggtatgg gaacgggtcg ggaagacttt agagagcagc
    24601 gcacaggtct ggccgctctg ggaaatttgc agcgccgcgc gcatacctgc gccaccggca
    24661 ccaatcacaa ctgcatcaaa ttctctgact ggcaatttca tcacaccccc cacaccacaa
    24721 cgaatccata aatcacgtaa accaccagtg caacgacaat caccagttgc agcatcaggc
    24781 gcaaggccag cggtttaacg tagtcggtca acacctgcca catgccgatc caggcatgaa
    24841 tcaagataga aaacagcgcc agcagggtga acactttggt gaacgcagag gcgaagaaac
    24901 cgatccagac ttcatatgtc agttcgccac tggtggcgaa aaaaccgacc atataaatga
    24961 tgtagagcgt caggacgata gcggtagcgc gaacgaggat gaaatcatgt acgccattgc
    25021 gtcctaatgc ggaggcgttg cttaccatac gaggactcct gcgagaagtg aaagcacgac
    25081 agtaataaca aaggagattt tggcggagcg tttacccgct tcgaatgttt cttccagata
    25141 gccaaaatcc atcatcatgt ggcgaatacc tacgacgacg tgatacgcca gagcggtaag
    25201 gatgccccac atgataaatt tgacgaagaa gctgcccata atcgcggaag cttgctcgaa
    25261 accttcaggg gaagagaggc tggtacccag aagccacagc aggatgccca ctgcaacaaa
    25321 ggtgatcaca ccggaaacgc gatggagaat ggacgctatc gccgtgatgg ggaaccggat
    25381 ggtctgtagg tccagattaa caggtctttg ttttttcaca tttcttatca tgaataacgc
    25441 ccacatgctg ttcttattat tccctgggga ctacgggcac agaggttaac tttctgttac
    25501 ctggagacgt cgggatttcc ttcctccggt ctgcttgcgg gtcagacagc gtcctttcca
    25561 taactgcgcg tcatgcaaaa cactgcttcc agatgcgaaa acgacacgtt acaacgctgg
    25621 gtggctcggg attgcagggt gttccggaga cctggcggta gtataggctg ttcacaaaat
    25681 cattacaatt aacctacata tagtttgtcg ggttttatcc tgaacagtga tccaggtcac
    25741 gataacaaca tttatttaat ttttaatcat ctaatttgac aatcattcaa caaagttgtt
    25801 acaaacatta ccaggaaaag catataatgc gtaaaagtta tgaagtcggt atttcaccta
    25861 agattaactt atgtaacagt gtggaagtat tgaccaattc attcgggaca gttattagtg
    25921 gtagacaagt ttaataattc ggattgctaa gtacttgatt cgccatttat tcgtcatcaa
    25981 tggattcttt acctgcaagc gcccagagct ctgtacccag gttttcccct ctttcacaga
    26041 gcggcgagcc aaataacaaa cgggtaaagc caggttgatg tgcgaaggca aatttaagtt
    26101 ccggcagtct tacgcaataa ggcgctaagg agaccttaaa tggctgatac aaaagcaaaa
    26161 ctcaccctca acggggacac agctgttgaa ctggatgtgc tgaaaggcac gctgggtcaa
    26221 gatgttattg atatccgtac tctcggttca aaaggtgtgt tcacctttga cccaggcttc
    26281 acttcaaccg catcctgcga atctaaaatt acttttattg atggtgatga aggtattttg
    26341 ctgcaccgcg gtttcccgat cgatcagctg gcgaccgatt ctaactacct agaagtttgt
    26401 tacatcctgc tgaatggtga aaaaccgact caggaacagt atgacgaatt taaaactacg
    26461 gtgacccgtc ataccatgat ccacgagcag attacccgtc tgttccacgc tttccgtcgc
    26521 gactcacatc caatggcagt catgtgtggt attaccggcg cgctggcggc gttctatcac
    26581 gactcgctgg atgttaacaa tcctcgtcat cgtgaaattg ccgcgttccg cctgctgtcg
    26641 aaaatgccga ccatggccgc gatgtgttac aagtattcca ttggtcagcc atttgtttat
    26701 ccgcgcaacg atctctccta tgccggtaac ttcctgaata tgatgttctc cacgccgtgc
    26761 gaaccgtatg aagttaatcc gattctggaa cgtgctatgg accgtattct gatcctgcac
    26821 gctgaccatg aacagaacgc ctctacctcc accgtgcgta ccgctggctc ttcgggtgcg
    26881 aacccgtttg cctgtatcgc agcaggtatt gcttcactgt ggggacctgc gcacggtggt
    26941 gctaacgaag cggcgctgaa aatgctggaa gaaattagct ccgttaaaca cattccggaa
    27001 tttgttcgtc gtgcgaaaga taaaaatgat tctttccgcc tgatgggctt cggtcaccgc
    27061 gtgtacaaaa attacgaccc gcgcgccacc gtaatgcgtg aaacctgcca tgaagttctg
    27121 aaagagctgg gcaccaaaga tgacctgctg gaagtggcta tggagctgga aaacatcgcg
    27181 ctgaacgacc cgtactttat cgagaagaaa ctgtacccga acgtcgattt ctactctggt
    27241 atcatcctga aagcgatggg tattccgtct tccatgttca ccgtcatttt cgcaatggca
    27301 cgtaccgttg gctggatcgc ccactggagc gaaatgcaca gttacggtat gaagattgcc
    27361 cgtccgcgtc agctgtatac aggatatgaa aaacgcgact ttaaaagcga tatcaagcgt
    27421 taatggttga ttgctaagtt gtaaatattt taacccgccg ttcaaatggc gggttaattt
    27481 ttatatgcct aaacacaaaa aattgtaaaa ataaaatcca ctatcggaca ttcatatatt
    27541 ctaaaaaaaa atatatcggt ataaattata aacaaatcga tgatttttaa tcatctatct
    27601 tcctctcagt tacatgaata aaccaccaaa tatagcgcac ccctataatt tccaatactt
    27661 tattcatcaa acatttaatt cataccatca attaattact tttacattta ataaaattaa
    27721 taatattttt aagaaaaact a
//
LOCUS       DADLXX010000041        27538 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLXX010000041 DADLXX010000000
VERSION     DADLXX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.041"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JNX27_001768"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JNX27_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7883007.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JNX27_001769"
     CDS             complement(1614..1958)
                     /locus_tag="JNX27_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7883008.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNX27_001770"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNX27_001770"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7883009.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNX27_001771"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNX27_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY7883010.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDIYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JNX27_001772"
     CDS             complement(6144..6842)
                     /locus_tag="JNX27_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7883011.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNX27_001773"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNX27_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7883012.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JNX27_001774"
     CDS             7537..8574
                     /locus_tag="JNX27_001774"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7883013.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNX27_001775"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNX27_001775"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7883014.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JNX27_001776"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JNX27_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7883015.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNX27_001777"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNX27_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7883016.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JNX27_001778"
     CDS             10745..11626
                     /locus_tag="JNX27_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7883017.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNX27_001779"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNX27_001779"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7883018.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JNX27_001780"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JNX27_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7883019.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNX27_001781"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNX27_001781"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY7883020.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JNX27_001782"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JNX27_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7883021.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JNX27_001783"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JNX27_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7883022.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JNX27_001784"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JNX27_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7883023.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JNX27_001785"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JNX27_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7883024.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHACLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JNX27_001786"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JNX27_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7883025.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JNX27_001787"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JNX27_001787"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7883026.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JNX27_001788"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JNX27_001788"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7883027.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JNX27_001789"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JNX27_001789"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7883028.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JNX27_001790"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JNX27_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7883029.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JNX27_001791"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JNX27_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7883030.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNX27_001792"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNX27_001792"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7883031.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JNX27_001793"
     CDS             24893..25273
                     /locus_tag="JNX27_001793"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7883032.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JNX27_001794"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JNX27_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7883033.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNX27_001795"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNX27_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7883034.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27538
                     /locus_tag="JNX27_001796"
     CDS             26442..>27538
                     /locus_tag="JNX27_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY7883035.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6793 a         6856 c         7220 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaagcgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa atatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgcca ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag gcagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaattg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgatac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcatgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagt atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc actggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgtaaa
//
LOCUS       DADLXX010000042        26933 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLXX010000042 DADLXX010000000
VERSION     DADLXX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.042"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..437
                     /locus_tag="JNX27_001797"
     CDS             <1..437
                     /locus_tag="JNX27_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY7883036.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNX27_001798"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNX27_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7883037.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="JNX27_001799"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="JNX27_001799"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7883038.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWHQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="JNX27_001800"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="JNX27_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY7883039.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="JNX27_001801"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="JNX27_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7883040.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNX27_001802"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNX27_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7883041.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="JNX27_001803"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="JNX27_001803"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7883042.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="JNX27_001804"
     CDS             complement(7412..8227)
                     /locus_tag="JNX27_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7883043.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="JNX27_001805"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="JNX27_001805"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7883044.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="JNX27_001806"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="JNX27_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7883045.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="JNX27_001807"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="JNX27_001807"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7883046.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNX27_001808"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNX27_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7883047.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="JNX27_001809"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="JNX27_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7883048.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHRVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="JNX27_001810"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="JNX27_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7883049.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="JNX27_001811"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="JNX27_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7883050.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="JNX27_001812"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="JNX27_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7883051.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="JNX27_001813"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="JNX27_001813"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY7883052.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNX27_001814"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNX27_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7883053.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="JNX27_001815"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="JNX27_001815"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY7883054.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVVTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="JNX27_001816"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="JNX27_001816"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7883055.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNX27_001817"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNX27_001817"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY7883056.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNX27_001818"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNX27_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY7883057.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="JNX27_001819"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="JNX27_001819"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7883058.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="JNX27_001820"
     CDS             24262..24876
                     /locus_tag="JNX27_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7883059.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="JNX27_001821"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="JNX27_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7883060.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="JNX27_001822"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="JNX27_001822"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7883061.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6166 a         6858 c         7431 g         6478 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcatcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatc
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc ggcagggcaa gtatttacag caccgtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 tgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gcggaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggctgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtggttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaa
//
LOCUS       DADLXX010000043        26319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLXX010000043 DADLXX010000000
VERSION     DADLXX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.043"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            118..1089
                     /locus_tag="JNX27_001823"
     CDS             118..1089
                     /locus_tag="JNX27_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7883062.1"
                     /translation="MRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1112..2623
                     /locus_tag="JNX27_001824"
     CDS             1112..2623
                     /locus_tag="JNX27_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7883063.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2648..3646
                     /locus_tag="JNX27_001825"
     CDS             2648..3646
                     /locus_tag="JNX27_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7883064.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTGLSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3679..4773
                     /locus_tag="JNX27_001826"
     CDS             3679..4773
                     /locus_tag="JNX27_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7883065.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4785..5657
                     /locus_tag="JNX27_001827"
     CDS             4785..5657
                     /locus_tag="JNX27_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7883066.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5705..6127)
                     /locus_tag="JNX27_001828"
     CDS             complement(5705..6127)
                     /locus_tag="JNX27_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7883067.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6224..7430)
                     /locus_tag="JNX27_001829"
                     /pseudo
     CDS             complement(6224..7430)
                     /locus_tag="JNX27_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNX27_001830"
     CDS             complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNX27_001830"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY7883068.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8249..8569)
                     /locus_tag="JNX27_001831"
     CDS             complement(8249..8569)
                     /locus_tag="JNX27_001831"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7883069.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8569..9087)
                     /locus_tag="JNX27_001832"
     CDS             complement(8569..9087)
                     /locus_tag="JNX27_001832"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7883070.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9084..10445)
                     /locus_tag="JNX27_001833"
     CDS             complement(9084..10445)
                     /locus_tag="JNX27_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY7883071.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10581..11471
                     /gene="hcaR"
                     /locus_tag="JNX27_001834"
     CDS             10581..11471
                     /gene="hcaR"
                     /locus_tag="JNX27_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7883072.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11631..12770
                     /locus_tag="JNX27_001835"
     CDS             11631..12770
                     /locus_tag="JNX27_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7883073.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNX27_001836"
     CDS             complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNX27_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7883074.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAVLAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(14233..15087)
                     /locus_tag="JNX27_001837"
     CDS             complement(14233..15087)
                     /locus_tag="JNX27_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7883075.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNX27_001838"
     CDS             complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNX27_001838"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7883076.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16154..16894
                     /gene="trmJ"
                     /locus_tag="JNX27_001839"
     CDS             16154..16894
                     /gene="trmJ"
                     /locus_tag="JNX27_001839"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7883077.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17164..17652
                     /gene="iscR"
                     /locus_tag="JNX27_001840"
     CDS             17164..17652
                     /gene="iscR"
                     /locus_tag="JNX27_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7883078.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17764..18978
                     /gene="iscS"
                     /locus_tag="JNX27_001841"
     CDS             17764..18978
                     /gene="iscS"
                     /locus_tag="JNX27_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7883079.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19006..19392
                     /gene="iscU"
                     /locus_tag="JNX27_001842"
     CDS             19006..19392
                     /gene="iscU"
                     /locus_tag="JNX27_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7883080.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19409..19732
                     /gene="iscA"
                     /locus_tag="JNX27_001843"
     CDS             19409..19732
                     /gene="iscA"
                     /locus_tag="JNX27_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7883081.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19828..20343
                     /gene="hscB"
                     /locus_tag="JNX27_001844"
     CDS             19828..20343
                     /gene="hscB"
                     /locus_tag="JNX27_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7883082.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20360..22210
                     /gene="hscA"
                     /locus_tag="JNX27_001845"
     CDS             20360..22210
                     /gene="hscA"
                     /locus_tag="JNX27_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7883083.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22212..22547
                     /gene="fdx"
                     /locus_tag="JNX27_001846"
     CDS             22212..22547
                     /gene="fdx"
                     /locus_tag="JNX27_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7883084.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22559..22759
                     /gene="iscX"
                     /locus_tag="JNX27_001847"
     CDS             22559..22759
                     /gene="iscX"
                     /locus_tag="JNX27_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7883085.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22818..24101
                     /gene="pepB"
                     /locus_tag="JNX27_001848"
     CDS             22818..24101
                     /gene="pepB"
                     /locus_tag="JNX27_001848"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7883086.1"
                     /translation="MTEAMKITISTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24243..25019
                     /gene="sseB"
                     /locus_tag="JNX27_001849"
     CDS             24243..25019
                     /gene="sseB"
                     /locus_tag="JNX27_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7883087.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25840..>26319)
                     /gene="sseA"
                     /locus_tag="JNX27_001850"
     CDS             complement(25840..>26319)
                     /gene="sseA"
                     /locus_tag="JNX27_001850"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY7883088.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6240 a         6557 c         7289 g         6233 t
ORIGIN      
        1 cgttcactaa accctattaa ctgtaatatt tgagcggcac gcgctgccgc tcatcacaaa
       61 ataattagaa taatccgttg ttaataaaaa ccctacagga ggcactatgc ctaaaaaatg
      121 agaacaaccc gtaatctatt attaatggct acgctattag gtagcgcact atttgccagg
      181 gctgcggata aagaaatgac cattggtgca atataccttg atacccaggg atattacgct
      241 ggagtgcgtc agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt
      301 gaaactaacg cccaggggga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg
      361 cgtaatgtcg atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt
      421 cgtcgcgcca gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag
      481 ggtgtcgata aatatgtctc ggcgtatctg gtcggcgatc cactggaatt tggtaaaaaa
      541 ctgggtaacg ctgccgccga ttattttatt gccaataaaa ttgaccagcc gaaaattgcc
      601 gtcatcaatt gcgaagcctt tgaagtttgt gtgcagcgac gtaaaggatt tgaagaagta
      661 ttaaaatccc gcgttcccgg cgcgcaaatt gtcgctaacc aggaagggac tgttttagat
      721 aaagcgattt ccgttggtga aaaactgatt atctccacgc cggatctcaa cgccattatg
      781 ggggagtcgg gcggtgcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc
      841 ggaaaaattg ctgttttcgg ttcggatatg acaaccgaaa ttgctcagga gctggaaaac
      901 aatcaggtgc tgaaagcggt ggtggatatt tccggtaaga aaatgggcaa tgctgttttc
      961 gcgcaaacat tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca
     1021 atcgatctct ataccaaaac ggaagatggt aaacagtggc tggcaacgca cgttgatggt
     1081 ctgccctaat cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt
     1141 agcaaaagtg gtggcaggaa ataagcgtta ccccggcgtc gttgcgctgg ataacgttaa
     1201 cttcacgctc aataaaggcg aagttcgtgc gctgttaggt aaaaacggcg cgggcaaatc
     1261 gactctcatt cgaatgctta ccggcagcga acgcccggat agcggtgata tctggattgg
     1321 cgagacgcga ctggaaggtg acgaagctac gttgactcgc cgtgccgctg aactgggggt
     1381 tcgtgcggtt tatcaggaat taagtctggt ggaagggctg acggtggcag aaaacctctg
     1441 ccttggtcaa tggcctcgcc gcaacggcat gattgattac ctgcaaatgg cgcaggatgc
     1501 ccaacgttgc ttacaggcgc tgggcgttga cgttagtcct gaacaacttg tttcaacgct
     1561 aagcccggcg caaaagcaac tggtggaaat tgcacgggtg atgaagggcg agccgcgcgt
     1621 ggtcattctt gatgaaccca ccagctcgct tgccagtgca gaagttgaac tggtgatcag
     1681 tgcggtgaaa aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga
     1741 agaaatccgc cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga
     1801 tgtgatgctc gaaaacacct ccacgcatca tattgtgtcg ctaatgctag ggcgtgatca
     1861 cgttgatatt gcgccggttg cccctcagga aattgtggat caggccgtac tggaagtccg
     1921 tgcgttacgc cataagccca agctggagga tatcagcttt acgctacgtc gtggcgaagt
     1981 gcttggcatt gctggcctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg
     2041 gctggagacg tatgaacagg gcgaaattgt tatcaacggc gagaaaatca tgcgccccga
     2101 ttacggcgac atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg
     2161 gatcattccc tggctgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag
     2221 caccaacggt gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg
     2281 gatgacggtc aaggctgcca gtagcgaaac acccatcggc acgctttctg gtggcaatca
     2341 gcaaaaagtg gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga
     2401 gccaacgcgc ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgtgagct
     2461 ggctgccgaa ggaaaaagcg tggtgtttat ctccagtgaa gtggaggaat tgccgctggt
     2521 gtgtgaccgc atcctgttat tacagcacgg cacgttctcg caggagtttc actcgccggt
     2581 caatgtggat gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa
     2641 cctgaagatg tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca
     2701 atttgtcagt cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct
     2761 ggtcttctcc ctgaacgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg
     2821 cgatgccgcc accattggga ttgccgcctg ggcgatgacg ctgattatta tctccggtga
     2881 aattgatgtc agcgttgggc cgatggtggc ttttgtctcg gtgtgcctgg catttttgct
     2941 gcaatttgaa gttccgttgg cgattgcttg tctgctggtg ttgctgttag gcgcgctgat
     3001 gggaacgctc gccggggtgc tgcgcggcgt gtttaacgta ccaagtttcg ttgccacact
     3061 ggggctgtgg agcgctctgc gcggaatggg gctgtttatg accaacgcac agccagtgcc
     3121 gattgacgaa aacgaggtgc tggactggct gggcggacaa tttctcggtg tgccagtatc
     3181 cgcgctgatc atgatggtgt tgtttgcgct gtttgtgttc attagccgaa aaaccgcctt
     3241 cgggcgctcg gtttttgccg ttggcggtaa tgccacggcg gcgcagttgt gcggtattaa
     3301 cgttcgtcga gtgcgcattc ttatctttac cctttcggga ttattagcgg cggtgaccgg
     3361 cattttgttg gcggcacgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt
     3421 tgacgtcatc gccgcggtcg tggtcggcgg tacgggactt tccggcggtc gcggttccct
     3481 gttcggtaca ttgcttggtg tgctggtgat tacgctaatc ggtaacggtc tggtgctgct
     3541 cggtattaac tcctttttcc agcaggtggt acgcggcgtc atcatcgtgg tggcggtgct
     3601 ggcgaatatc ttgctgaccc agcgaagcag taaagcgaaa cgctaaacct taacgcccct
     3661 ggcgcgggtg cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg
     3721 atgctggcag cttatttacc aggaaattca accgtcgatc tgcgggaagt tgcggtgccg
     3781 acgccgggga ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat
     3841 gtccactata tctatcatca acaccgtgcc acggcggcgg cacccgataa accgttatac
     3901 cagggattta tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctgc
     3961 cgccatttta aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc
     4021 ccgaactgcc gccgcggctt tcctatctct tgtactggca aaggaaaagc ggcttacggc
     4081 tggcagcgtg acggcggtca tgccgaatat ctgctggcgg aagaaaaaga tctgatcctc
     4141 ctgccggatg cgctgagcta cgaagatggt gcgtttatca gttgcggcgt tggcacggcc
     4201 tatgaaggga ttttgcgcgg cgaagtttcc ggcagcgata acgtgctggt ggtcggtctg
     4261 ggggcggtcg gcatgatggc gatgatgctg gcgaaaggtc gcggtgcaaa acggatcatc
     4321 ggcgttgata tgctgccgga acgtctggcg atggcaaaac agttaggggg gatggatcac
     4381 ggctatttag caaccaccga aggtctgccg cagataattg ctgaacttac ccacggtggc
     4441 gcggatgttg cgctcgattg ttccggtaat gccgcaggtc gcttgctggc actgcaatcc
     4501 actgctgact gggggcgggt ggtttacatt ggtgaaaccg gaaaagtgga attcgaggtc
     4561 agcgctgatc tgatgcacca tcaacggcgg attatcggct cctgggtgac cagtctgttc
     4621 catatggaaa aatgcgccca cgatttgact gactggaagc tgtggccgcg taacgccatt
     4681 acccatcgct tctcgctaga acaggcaggt gatgcctatg tgctgatggc gagcggcaaa
     4741 tgcgggaaag ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct
     4801 tatcccacgg gtcgctaaag ctggacgttt ctgaccaggg cggcgtaatc gaaggcttct
     4861 ggcgcgatac gacgccgctc ttacgtcctg gtaaaaaaag cggtgtggca accgatgcct
     4921 catgttttcc gctggtgcca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg
     4981 ggcgtgagta tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg
     5041 gctggctggg cgagtggcaa tgtgtctcgc acagcgatga tagtttgtgt ctggtgtatg
     5101 aacatcgcag cggtgtctat cactatcggg taagtcaggc gtttcattta acggcggata
     5161 cgctgacggt gacgctctct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg
     5221 gctggcatcc ttattttccg ttgtcaccac aaacgcggat tcaggcgcag gcgagcggtt
     5281 actggctgga acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac
     5341 tggattttaa ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat
     5401 ggaatggtca ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga
     5461 cgccacctgc accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg
     5521 cgtttgattt cttctgtctg gaaccgatga gccatgcgcc ggatgatcat catcgcccgg
     5581 aagggggtga tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc
     5641 gggtagagtg gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc
     5701 tttcctaacg ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg
     5761 cgccagcgat actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg
     5821 tcatatccca gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca
     5881 ccgccagcgg acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa
     5941 ctaccgcaat aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga
     6001 ccgtaccgtc aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg
     6061 cgatacgggc aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg
     6121 tgttcataac caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac
     6181 ctcgccacgc cccaggcatt gagatattcg ctaattatcc tggttacagt gatttaagcg
     6241 cgatgttctc atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc
     6301 gaattgggcg aatctcacgc ccctgattca gcgtgaccgc accgataagc acgccgtttt
     6361 gcagattaaa ccaaatcgcc ttctgagttt ccgggttgcc acgacaaagc cagtcatcgc
     6421 cacgcatatc gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg
     6481 gcggcagtag cggtagccct aacattgcgg cagcggcaat ttgcgcctgg ttattggtgt
     6541 tttcccagct ttcgcagcgg tgtagtgcac cattatcaag acgagtgatt gccacatcgc
     6601 caccggcaaa gatcgcggga tcgcaggtgc ggcaagcctc atcaatgaca atgccattgg
     6661 cagtatcaag gttggcctcg cgagccagtt gctcgttggc gctgatacta ataccgtaaa
     6721 tcaccacatc agcctgaagc gtttccccac tttgcagcgt cagttctact ttttcaccat
     6781 cgaccacatg ttcaatggca ttattgagca gaatgcgcac accagcctgc tgatggcgct
     6841 gtaaaagata gcgttgcacg ggcggtggtg cattacggcc catgacggtt gccgccagtt
     6901 caatcactgt caccttacat ctgcgctgcg ctgcgctgcg tggcgctggc agccagttcc
     6961 agaccaatag ttccggcacc gacaatcacg actgaccgtt cgggctgcag aacttctcgc
     7021 agtctggcgg catcaccggc atggtgtagg gtaaagcagc gttctcccag tgcatcaagc
     7081 aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt
     7141 tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg
     7201 gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg ctgtaactgc
     7261 ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg aagatgtcgc
     7321 tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc
     7381 attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct
     7441 taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg
     7501 ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacggc
     7561 cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc
     7621 ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg
     7681 tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa
     7741 gccagttggc gaataagtcc ggttgcggca tgtttactgg tggtgtacag cgggccaccg
     7801 ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc actggcgatt
     7861 aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac gttaaacagc
     7921 tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa ccagtgaggc attgtgatcc
     7981 cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag gatctgatcg
     8041 accgcgcgtt gataatcggc ataacaggtc acgttacctt ccaccgccag aatatgttcg
     8101 ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgc ggcaacctgc
     8161 gcgccttctt cgataaatcg ctcgaccagc gccagcccta atcccgatcc gccgccggta
     8221 ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa
     8281 tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg ggcaggcata
     8341 acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc accgtggcgt
     8401 catcttccag atacccttct gacatcgacg cattaccatg gctgcaacga tcgttaatgg
     8461 cataaaactc gccgcccacg ttgaacaagg cgatgacggg cgaggtatct atccggagag
     8521 cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac
     8581 actcaggtta tgggaagtga tcaccgcttg atccaggacg atatcccgtt ccagcaagcg
     8641 ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc
     8701 atcgcgctct ttttgtgccc gataaagtag ataattcacc ctcacggcaa atacgtttgg
     8761 gatgtcggtt tcgcttacct ggcagttgct gattaagtga cgagtgcgtg acggcggctc
     8821 ttctgcccag gccatgcccg tttccagacg ggcgattcgc cgctccagct ggtctttggt
     8881 gtcattgaaa atccaggtcg tcggcggctg gacgcctttg cgacggtcgc gtgtttgcgc
     8941 gttaaccgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgtgcca accagtcacg
     9001 aaatttccag tcatccagca gactggcttc gtgaaagaga aactggctaa tgcggtgatg
     9061 taactccagt gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta
     9121 tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca
     9181 cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccgtc gtcgcggcgc
     9241 ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggccttta
     9301 agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga
     9361 ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg
     9421 tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg ccagacgcgg
     9481 agtgtggcag tgccgtttag ccatgaaagc gtggggaata tattgttatg acccgccagg
     9541 cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag
     9601 cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg
     9661 ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg
     9721 gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc taatacctga
     9781 acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg ctctgccggg
     9841 aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc ggtgccgcct
     9901 tcgcgacgat ccagcatgcc atccagatac caggcaatgt cacccaggta atcatgcagg
     9961 cccggtgcgc tggtatccca gttgccaaaa atcagtcctt tataactctc cacacaagga
    10021 acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc
    10081 ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg gcaggtaaag
    10141 gcgcgagtgt tgccacaatc tgcataactc acacgcatgg cccggtggcg gcattggtta
    10201 agaaacgcct tgatgctgcc gtctttctgg cgcaccacga caactgcatc ttctcccatg
    10261 taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag aaatagccag
    10321 caacgaccga aaatacgctc aagctccagt tggtaaatgt ccgggtcggt ataaatacgc
    10381 ggagtgaccc gaccgttttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg
    10441 gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg tgccataagg
    10501 atgttgctgg ttggttgcta atatgtgaaa tagttaattt ctaccagctg agacgttttt
    10561 tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc
    10621 aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagta
    10681 gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg gataagcgca
    10741 aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac
    10801 aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca
    10861 ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg atgtttcgtc
    10921 tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa caggaggaaa
    10981 aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga
    11041 ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt
    11101 tagcacatga aaaagagatc accgccgcgc aactggatgg cgtgaatttc gtcagtaccg
    11161 atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca
    11221 gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggca
    11281 tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc gggcaggttg
    11341 tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg tggaagaaag
    11401 gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg
    11461 taacggcata attacgcatt atttttgcaa catgcgccgc ggatcgcagt tttttagtgc
    11521 ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg
    11581 caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc
    11641 aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttt
    11701 tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc atcggcctgt
    11761 tattgggggc aggtctggtt gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca
    11821 gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc actgctgcca cttctctttg
    11881 ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta
    11941 acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc
    12001 agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt gtcattggct
    12061 cggcgctgac gggcaaactg gtcactatgt ttgattatcg ggtgatcctc gtgctgttga
    12121 cgttgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag
    12181 gggcaagccg ccagcaggag agcaccggtt ggtctgcgtg gttggcgctg gttcgccaga
    12241 actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc tattacggtt
    12301 ttagcgccat ttactggcag gcagctggct actcggcctc ggcggtgggg tatttgtggt
    12361 cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt
    12421 gtagtgctcg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta
    12481 tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat tgcggcacct
    12541 tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccaggtt agcgaagtca
    12601 tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg
    12661 ttttcgccgg tttcctgtat caatatctgg gccacggtgt gttctgggta atggcgctgg
    12721 tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt
    12781 ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt ccgtatagat cagcggtggt
    12841 gaaaagagcg gtaacggcat ggtataaggc gcaataatca gtgccgcgcc acgcggagcg
    12901 cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag taacgttagt
    12961 tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata
    13021 atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat caccgcgacc
    13081 agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcacc
    13141 gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga actcttccgg cagggtatta
    13201 tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtggat ataaagctga
    13261 tcacataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc
    13321 agacgtttga tagactgacg caattgtctg tcccgctgat gcgcatcccg cagtggatcg
    13381 ggaacccgca acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacatgc
    13441 tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag
    13501 gattccgccc agcgacgttg gagcggatta aactgaggcg taattccagc gtggtgctgc
    13561 agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc aaagtgtttt
    13621 tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta
    13681 cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg gggtgaattg gctggtaata
    13741 aatgacggac acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta
    13801 agcactgtac cttcaacccg gtagctaccg tcataaccag tcgtcagtgt gagttgatga
    13861 tagcgcagga tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca
    13921 ccattaagct cgctgaaggt tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc
    13981 aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc
    14041 atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct cagggaaatg
    14101 gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatttta
    14161 aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct
    14221 agcgatcatg ggttagttct gcggattcaa cgcgctaagg ataaagtcca ccatctgttc
    14281 gcgataaaca ttgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag
    14341 gatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat
    14401 aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc gggctgacgc tggcgatgta
    14461 attttcgccg ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc
    14521 gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc
    14581 accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga acagcaccag
    14641 ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt gcgtatcgtc
    14701 cagcaatccg gcctgggacg gagtgccttt tgatttacca aacccgcgat aatcaaacat
    14761 aaaaacgttg aaattacgct caggtaacca actgaccagc ggccagtggg cggacatatt
    14821 tccggcattg ccgtgagcat gaatgatggt tgcgatggcg ttgtcagcag ggctcgtcga
    14881 agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt cgacggattc
    14941 cgccgaccag gggtcgggac cgtaaatttt gtcgtcagga taatagaaga agttgatggc
    15001 aacgcgcagc actaaataaa cgcagaaggc aacaacaaag agaataaaca gaattttggg
    15061 aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta tcatccatta
    15121 atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcggtgagga agtatttcgg
    15181 aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc cgcctgagtc attaacgctt
    15241 cagagcgtcg cttaactcgt cacgcatgtt cgccagtatg gctttcacaa cacgcgggtt
    15301 accagcaacg atgttaccgg tcagcatata gttatgacca ccggtgaagt cgctgacgat
    15361 gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac gcagaccgat
    15421 ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc agatccagcg ccgcagaacc
    15481 ggtacgacgg aagtctgcac attcgttgaa cagcttgccg acgatgttga tgtaggtagt
    15541 ggcgtactgt tttgctttga acgggaagcc ggtcgccaga atggtaccgt cgagatcgcg
    15601 agcggtgcta ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt
    15661 gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc ctttgatacg
    15721 aacggcgata gataccgcga agtgcggcag acgtttgata aagttggtag taccatccag
    15781 tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt
    15841 gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc
    15901 tttatctacg ttggtcacga aatcgttact gcctttctgg ctcgcttcta cagcgtccgg
    15961 ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt
    16021 cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag
    16081 tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag
    16141 atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg
    16201 caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt
    16261 taatccactg gtgaaacccg actcccaggc gattgccctg gctgcagggg ccagcgatgt
    16321 gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt
    16381 ggtgggcacc agcgcacgct cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg
    16441 cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt ttggtcgtga
    16501 gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg cgattgcggc
    16561 aaacccggaa tacagttcgc tgaacctggc aatggcggtt caggttattg cctatgaagt
    16621 gcgtatggcc tggctggcaa ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc
    16681 gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc
    16741 aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt
    16801 tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg cgcgggattc tggcttctat
    16861 tgagcagcag aataaaggta acaaggccga ataacagtgg ttgcctgatg cgacgcttaa
    16921 cgcgtcttat caggcctaca atgtacagaa tcgtaggtcg gataaggtgt tcacaccgca
    16981 tccgacaaca ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa atacccgact
    17041 aaatcagtca agtaaatagt tgaccaattt actcgggaat gtcagacttg accctgctat
    17101 gcaatacccc cacttttaca ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa
    17161 gacatgagac tgacatctaa agggcgctat gccgtgaccg caatgcttga cgttgcgctc
    17221 aactctgaag cgggcccggt accgttggct gatatttccg aacgtcaggg aatttccctt
    17281 tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt
    17341 ggaccaggcg gtggttatct gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg
    17401 attagcgccg ttgacgaatc tgtagatgcc acccgttgtc agggtaaagg cggctgccag
    17461 ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt
    17521 tttctcaaca acattacttt aggcgaactg gttaataacc aggaagtgct ggatgtgtct
    17581 ggtcgtcagc atactcacga cgcgccacgc acccgcacac aagacgcgat cgacgttaag
    17641 ttacgcgctt aataaaaaga attcagaatc aggccggagt gctaaatact ccgtaaacac
    17701 ggtcgtacat ccagccggta gcctgattcc ttgcattgag tgatgtacgg agtttataga
    17761 gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt
    17821 gccgagaaaa tgatgcagtt catgacgatg gacggaacct ttggtaaccc ggcctctcgt
    17881 tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc
    17941 gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac
    18001 aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc
    18061 agcaaaaccg aacacaaagc ggtactggat acttgccgtc agctggagcg cgaaggtttc
    18121 gaagtcacct acctggcacc gcagcgtaac ggtattatcg acctgaaaga actcgaagcg
    18181 gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg
    18241 gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt
    18301 gatgcaaccc agagcgtggg caaactgcca atcgacctga gccagttgaa agttgacctg
    18361 atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtgcgt
    18421 cgtaaaccgc gtgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg
    18481 cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaagccta tcgcatcgca
    18541 aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac
    18601 ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac
    18661 attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac
    18721 ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg
    18781 cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt
    18841 tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt
    18901 ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc
    18961 gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa
    19021 aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgataa caacgacgag
    19081 aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtaatgaa gttgcagatt
    19141 aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttcc
    19201 gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg
    19261 caggcgatca aaaacaccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac
    19321 tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt
    19381 gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc
    19441 gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag
    19501 aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga
    19561 agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt
    19621 tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac
    19681 caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata
    19741 ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc
    19801 gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc
    19861 cgttatcaac tcgataccca ggcactgagc ctgcgttttc aggatctaca acgtcagtat
    19921 catcctgata aattcgccag cggaagcctg gcggaacaac tcgccgccgt acagcaatct
    19981 gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg
    20041 ctttctttac acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc
    20101 ctgatggaac agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa
    20161 gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg
    20221 atggttgaac agttagacaa cgaggcgtgg gacgcggcgg cggatacggt gcgtaagctg
    20281 cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt
    20341 taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc
    20401 gccgcatcag cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc
    20461 gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc
    20521 atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc
    20581 agcgcttgac accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc
    20641 tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct
    20701 gccgatgatt gaaacggcgg cggggctact gaacccggtg cgcgtttctg cggacatcct
    20761 caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat
    20821 caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct
    20881 ggcggggttg catgttctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg
    20941 gctggattcc ggtcaggaag gcgtgatcgc tgtttatgac ctcggtggcg ggacgtttga
    21001 tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcaaccg gcggtgattc
    21061 cgcgctcggc ggcgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcgga
    21121 cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc
    21181 caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga
    21241 aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc
    21301 ttgtcgtcgc gcgctgaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat
    21361 ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg
    21421 tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc
    21481 ggatattctg gtgggtaaca agccagacag cgaaatgctg ctgcttgatg tgatcccact
    21541 gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac
    21601 tattccggtg gcccgcgctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc
    21661 tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt
    21721 tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca
    21781 ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc
    21841 gtctattcag gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa
    21901 agactcaatg agctatgccg agcaggacgt aaaagcccgt atgctggcag aacaaaaagt
    21961 agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt
    22021 aagcgccgca gagcgtcagg tcattgacaa tgctgccgct cacctgagtg aagtggcgca
    22081 gggcgatgat gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga
    22141 tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga
    22201 cgaggtttaa tatgccaaag attgttattt tgcctcatca ggacctctgc cctgatggcg
    22261 ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agcgctgcgt aacggtatcg
    22321 agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg
    22381 aaggttttga ctcactgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct
    22441 ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag
    22501 tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat
    22561 gggacttaag tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga
    22621 tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga
    22681 tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg
    22741 gctggacgaa gccgagtgaa tgaacgggct gcctgggggt agcccgtttt gctgttaaac
    22801 gaataaggat aactaaaatg acagaagcga tgaagattac catctctacc caacctgccg
    22861 acgcgcgctg gggagaaaaa gcaacttaca gcattaataa tgacggcatt accctgcatt
    22921 tgaacggggc agacgatctg gggctgatcc agcgtgcggc ccgcaagatt gacggtctgg
    22981 gcatcaagca tgttcagtta agcggtgaag gctgggatgc ggatcgctgc tgggcattct
    23041 ggcaaggtta taaaggcccg aaaggcacgc gtaaagtgga gtggccggat ctggacgatg
    23101 cccagcgcca ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca
    23161 acgcaccggc agaagaattg ggaccatcgc aactggcaca gcgtgctgtt gatctgatca
    23221 gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc
    23281 aaggttatat ggggctgcac actgtcgggc gcggttcaga acgttctccg gtattgctgg
    23341 cgctggatta caacccaact ggcgataaag aagcaccggt gtacgcgtgc ctggtaggta
    23401 aaggtatcac ttttgactcc ggcggctaca gcatcaaaca gactgcgttt atggactcga
    23461 tgaagtcgga catgggcggc gcggcaacgg ttaccggggc gctggcattt gccattacgc
    23521 gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc ggataacctg attagcggca
    23581 atgcgttcaa gctgggcgat atcatcacct atcgcaacgg taaaaaagtt gaagtgatga
    23641 acactgatgc cgaagggcgt ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga
    23701 aaccggaaat gatcattgat gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta
    23761 atgattatca cgcgctgttc agttttgacg atgcgcttgc cggtcgttta ctggcgagtg
    23821 cctcacaaga gaacgaacca ttctggcgtc tgccgctggc ggaattccac cgcagccagc
    23881 tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga
    23941 gcacggcggc gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata
    24001 tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg
    24061 gacttggtgt gcgcacgata gctaatctgt taacggcgta aactaatatg ccggatgcgg
    24121 cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa aaaattgcga atcccttgta
    24181 ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa cgttgaatga attgtggttc
    24241 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    24301 cgcaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg cctggtacgg
    24361 cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga tttacagcac tgggaaaaag
    24421 aagacggcac cagcgtcatt ccttttttca cctcgttaga agcgcttcaa caggcggttg
    24481 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    24541 aaacgctctt ccttaatgcc aaactgccga ccggtaaaga atttatgccg cgtgaaatca
    24601 gtttgttgat tggtgaagag ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg
    24661 aatcgctgat attatcggaa gtcgcagagc cgccagcaca aatgattgat tcactcacca
    24721 ccttatttaa aaccattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    24781 aagaggcaca gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta
    24841 ttcaggcgac gggaagtgta gcgaccgata cattacctgg cgatgaacca atcgatattt
    24901 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat
    24961 tctatgaacg tcgctggggt ggttttttgc gtgactttaa acaaaaccgg ataatctaaa
    25021 atatgccgcc ctaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccac
    25081 cagtaaattg tcgccggaga gactccggtt attatttact tgggaaaaca gcgcaccaga
    25141 aacaataagt acgatacaga agcaccgtat cttcattgtg ctgtcgtgat gcgaagaccg
    25201 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    25261 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg
    25321 ccgcagtgga taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga
    25381 gttactggcg agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg
    25441 ctggaaaggc cgcgaaccgg accagcaaca aataaccgcc tgattttggc ggttttttgt
    25501 tttttaaagg aaagtaaatt tacatgttta agtaatatct tttaccggtt agaattttta
    25561 attttgagca ggatttgttt atatttgtct gaattcggac taataagcca ggttatttta
    25621 ttaccgaaca cggcggaaag ggccggtgtc gataatacgc taaataaaca ataattactc
    25681 tcttttgctt gacaaaaaga gagttactgg tgagtattgt tttactgtga agcacctgcg
    25741 ttgctcatgt aacacaagtc aacctgctgg aaaggccgcg aaccagacca gcaataaaaa
    25801 aacgccaaat tcggcggttt tttattgcct gtaaaatact tatttcactg gctcaaccgg
    25861 taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg tacagtttca cgtttggcac
    25921 atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt acaccagagc cgcagctgac
    25981 gataatcggt ttgtcatagc tgacgccgcg accaaaaaat atcgcatcca gttcatcggt
    26041 cgtttttagt tcgccttcgc gcaccagttc cgtccacgga acgttcaatg caccgggaat
    26101 atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg
    26161 gcgggcatca ataatttgcg ccgtattttc atggcttgcc aataacacat cggttacttt
    26221 caccactgct tcaggattaa acgcggcgtt aaactctcct tccggcagct ctactgcacc
    26281 ttcttctaat agcaggtcat cgcgctgcca gcctgcaag
//
LOCUS       DADLXX010000044        26266 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLXX010000044 DADLXX010000000
VERSION     DADLXX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.044"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(45..263)
                     /gene="osmB"
                     /locus_tag="JNX27_001851"
     CDS             complement(45..263)
                     /gene="osmB"
                     /locus_tag="JNX27_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7883089.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(532..1281)
                     /locus_tag="JNX27_001852"
     CDS             complement(532..1281)
                     /locus_tag="JNX27_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7883090.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(1371..1544)
                     /locus_tag="JNX27_001853"
     CDS             complement(1371..1544)
                     /locus_tag="JNX27_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883091.1"
                     /translation="MSEFDTQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(1692..3666)
                     /gene="pdeR"
                     /locus_tag="JNX27_001854"
                     /pseudo
     CDS             complement(1692..3666)
                     /gene="pdeR"
                     /locus_tag="JNX27_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            3720..3822
                     /locus_tag="JNX27_001855"
                     /pseudo
     CDS             3720..3822
                     /locus_tag="JNX27_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3900..5834)
                     /locus_tag="JNX27_001856"
     CDS             complement(3900..5834)
                     /locus_tag="JNX27_001856"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7883092.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5902..7029)
                     /locus_tag="JNX27_001857"
     CDS             complement(5902..7029)
                     /locus_tag="JNX27_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7883093.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(7173..7961)
                     /gene="fabI"
                     /locus_tag="JNX27_001858"
     CDS             complement(7173..7961)
                     /gene="fabI"
                     /locus_tag="JNX27_001858"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7883094.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(8329..8682)
                     /locus_tag="JNX27_001859"
     CDS             complement(8329..8682)
                     /locus_tag="JNX27_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7883095.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(8750..9556)
                     /gene="sapF"
                     /locus_tag="JNX27_001860"
     CDS             complement(8750..9556)
                     /gene="sapF"
                     /locus_tag="JNX27_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7883096.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(9558..10550)
                     /gene="sapD"
                     /locus_tag="JNX27_001861"
     CDS             complement(9558..10550)
                     /gene="sapD"
                     /locus_tag="JNX27_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7883097.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(10550..11440)
                     /gene="sapC"
                     /locus_tag="JNX27_001862"
     CDS             complement(10550..11440)
                     /gene="sapC"
                     /locus_tag="JNX27_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7883098.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(11427..12392)
                     /gene="sapB"
                     /locus_tag="JNX27_001863"
     CDS             complement(11427..12392)
                     /gene="sapB"
                     /locus_tag="JNX27_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY7883099.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(12389..14032)
                     /gene="sapA"
                     /locus_tag="JNX27_001864"
     CDS             complement(12389..14032)
                     /gene="sapA"
                     /locus_tag="JNX27_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7883100.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(14345..14590)
                     /locus_tag="JNX27_001865"
     CDS             complement(14345..14590)
                     /locus_tag="JNX27_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7883101.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(14724..16109)
                     /gene="puuP"
                     /locus_tag="JNX27_001866"
     CDS             complement(14724..16109)
                     /gene="puuP"
                     /locus_tag="JNX27_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7883102.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVHQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(16099..16263)
                     /locus_tag="JNX27_001867"
     CDS             complement(16099..16263)
                     /locus_tag="JNX27_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883103.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(16412..17829)
                     /locus_tag="JNX27_001868"
                     /pseudo
     CDS             complement(16412..17829)
                     /locus_tag="JNX27_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
     gene            18041..18805
                     /gene="puuD"
                     /locus_tag="JNX27_001869"
     CDS             18041..18805
                     /gene="puuD"
                     /locus_tag="JNX27_001869"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY7883104.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            18832..19389
                     /gene="puuR"
                     /locus_tag="JNX27_001870"
     CDS             18832..19389
                     /gene="puuR"
                     /locus_tag="JNX27_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7883105.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            19539..21026
                     /gene="puuC"
                     /locus_tag="JNX27_001871"
     CDS             19539..21026
                     /gene="puuC"
                     /locus_tag="JNX27_001871"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY7883106.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            21028..22308
                     /locus_tag="JNX27_001872"
     CDS             21028..22308
                     /locus_tag="JNX27_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7883107.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            22346..23611
                     /gene="puuE"
                     /locus_tag="JNX27_001873"
     CDS             22346..23611
                     /gene="puuE"
                     /locus_tag="JNX27_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7883108.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(23731..24708)
                     /gene="pspF"
                     /locus_tag="JNX27_001874"
     CDS             complement(23731..24708)
                     /gene="pspF"
                     /locus_tag="JNX27_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7883109.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLQDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            24875..25543
                     /gene="pspA"
                     /locus_tag="JNX27_001875"
     CDS             24875..25543
                     /gene="pspA"
                     /locus_tag="JNX27_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7883110.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            25597..25821
                     /gene="pspB"
                     /locus_tag="JNX27_001876"
     CDS             25597..25821
                     /gene="pspB"
                     /locus_tag="JNX27_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7883111.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            25821..26180
                     /gene="pspC"
                     /locus_tag="JNX27_001877"
     CDS             25821..26180
                     /gene="pspC"
                     /locus_tag="JNX27_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7883112.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            26189..>26266
                     /locus_tag="JNX27_001878"
     CDS             26189..>26266
                     /locus_tag="JNX27_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein D"
                     /protein_id="HAY7883113.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLT"
BASE COUNT         6601 a         6807 c         6813 g         6045 t
ORIGIN      
        1 aagcgtatta taattgcgag tcgataatcg acatttattt acgattattt accgacctgg
       61 tgaccaataa caccaccgac agctgcacca cctaatgtac ccaacgtact gccatcggtc
      121 agtactgcac cgcctaatgc ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta
      181 gaccagttag aacaggcact cagagacatt gccaaagtaa ttgccagaac agccgcggtc
      241 atttttttgc tcgttacaaa cataatactc tctcctgaat ttatgattca cggaagtaag
      301 ctctctataa ctataatagc taagaataag cctggtgaaa ttattccgtg aaatctgctc
      361 gcgcgcaggg ttatatcacg cacgtgataa ttacttcctg ttatatcgct gataataatt
      421 ttatatcttg agagtgttaa taacaggtaa atagtcttaa ttatcaacca ggaatcatct
      481 tagagcggat gatttgccaa actgcaaatc atccgtagag aagggaaatg gttaaatgtc
      541 aacgacgtga acggtgagtt tactgtgctc aagatgcatc agatcgctgg cgcgtatttt
      601 cgaatcggta atcacgcgat tgaatcgctc aacgggaccg atggagtatg aatgtacagc
      661 accaaatttc gagctgtcag tcaggactat cgcttcgcac tctttttcca gcacggcatt
      721 gaccacatcg gtgcgcatca tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc
      781 aataccaata aatgctttgc tgaaatgtac ctgctggatg cactggcgtg tcaaagggcc
      841 aaccatactt tcgctttttt tctggtacac gccaccgagc aaaatgactt cacaaggcgc
      901 gtctttcagc aaatgcgcga tgtagctgct gaccgtgatg atagtgacat ttttcttctg
      961 ctcccccaga gtccgggcga gcagggcatt gctgctgcca ttttcgataa agatggtttc
     1021 gcccggttga accagtgacg cggcaaactc ggcgagttca cgcttcagcg tatagttgct
     1081 catcatacgg gtttcgacgt catcactatc aagcgaaact gcaaagccat gtgcacggcg
     1141 gaggtaactc agtttttcga gggtgttgag atcctggcga atggtaactt cagaaactcc
     1201 agtggctttt gccagatcgg ttacgctaac ctgaccctgg tcaatgacca tctgtagaat
     1261 agtttgttgt cgggaattca tcgctgtaat tcttataacg ttataatact aaataaaaaa
     1321 atactgacgc gccgggcatc ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc
     1381 cacggtgtct tcgggatgtc tggcgtagat tcagcaacct ggcgcaagag ttctgcttta
     1441 agtatctcga gattatggat cgcagagtgg taatcgcctg ccacaaggat atctaacaca
     1501 atatcaatac gttcggcaac gcgctgggta tcaaattcag acatcaagga catccttata
     1561 gcaaaagggg gaaaatgaat aatgcaaaaa ttgccgctaa aagagaagtg tttaacagca
     1621 acggctaatt atcatccagg aatacgatat ataatgacgg gatatagcgc taagtatata
     1681 tattcatcta cttatgcgcg cttcagatag cgtttatacc agcgttcgaa ggcgacggcg
     1741 ggcatcggtt tggcaaacaa aaatccttgc cgctcattga tcccgttctt ggttaaaaaa
     1801 gcatcttcct ttgcactctc tacaccttcg gcgatcacct gaagattcaa tgcctgggcc
     1861 acagcgacga tcgcccggac cagtgactgc gagacaggtt gtttgtgaat atctcgaaca
     1921 aaaacctggt caagtttgat ggcatcgatc ggaaagcgcg ccagttgcga aagtgaagag
     1981 tagccggtac caaaatcatc cagatgcact tgcgcaccta gttggctaaa ttgttgaata
     2041 acagacagtg ccagttcatc attctcaatc agacaactct ctgtcagttc aacatctata
     2101 gggcagtatt caaaattgag ttcctggaga acctgtttca gggcggtgaa aatggtttga
     2161 tcggcgagct gacgtgcaga aatatttacc gccactcgca ggtttatgcc tttatcccgc
     2221 cactttgcca cctggcgtac gacatcgaga atcacccaac ggcctaaagg cacaattagc
     2281 cctgactctt cggcgtagga aatgaagtcc aacggtggaa tcaacccacg ttcaggtgac
     2341 tgccaacgta ctagtgcttc cagactgcgc acttcgccac gctaggtgat tttcgataac
     2401 caactgatcg ttttccagtg ctttacgcaa gttggtatcc agccagagat attcaaatac
     2461 ccgctgattc atttctggag taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt
     2521 gtacattgct gtgtcggcgt gacgaataat agtcgcgctg tccgaaccat gttcgggaga
     2581 gagcgaaata cctactgaac agctggtata aacttcaatt aaaccaatac gaaaggggag
     2641 gcgtaagcgg gttaaaattc gtgatgccat tgcttccagc gcgctttgtg aggtattgga
     2701 tgccagtacc agaaactcat cgccacctgg acgcgccaac acctggtcat gttcgagaca
     2761 gcttaaaata gccaatgaca cgtcgcgtaa taactggtca ccaaacaaat gcccataggc
     2821 gtcgttgacc tttttaaaat tatccaaatc aagatacaca accccaactt tattgttatc
     2881 tgcctgatta atagcgtgat cgattaactc ctgcattgcg ttacgattcg gcagtccggt
     2941 gatactgtcg gtatttgcca gaatacgcag tcgctcctga gtgcggcgct cttcggtaat
     3001 gtcggtaccg gaacagatta aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt
     3061 attgcgaaac agaaacagcc gctggccttt acgtgttggt atccacagtt cgacttcata
     3121 tgcattgccg cttcgaaaaa atacacggtt attgcgcctg gatgccgcag cttcacgacg
     3181 gctcataaac agtttaaaca cgctttgccc aatgacgtcg tgttctttca accctgtgta
     3241 atcttcacat aaccgattga agcgttggat attcccccgg ctgtcgagaa tcactattgc
     3301 ggaatgtgct tcagaaacta cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc
     3361 aaccgccggt gtgtcatgcc atgcagacgc gttgccagcc cattcccgtt tattgatttt
     3421 tcgtcctacc agatgctcag aaagatcgct ttcatcgacg gtcagactca tcatcaggct
     3481 ggaggtgatg accgtcattt ccctgatgcg agcagcctgt tcggcagata actgcaatgt
     3541 acgatcaggt tctgtggttt tggtggtaga aaagcgcaaa gcatcgctgc tttccgtcaa
     3601 ccgccagtat ggattgtgcg atccgagaaa gttgtacaac gttgtggact ccctaacggt
     3661 tttcatgatg catatcctcc ggttaacagc ggagtccgcg gttaaataaa aggaacaaca
     3721 tgacttacat gaaattaacg gcggataaac gcattgtttt ttatatattt atttgtaatc
     3781 cagttttgga aaaacgccag ttttcaaacg aaagtcagtt aaaaaatctg cctggatata
     3841 acgaaggtag agcggggaaa taaacggccc atccatgagg aatgggccgt gaaaggagat
     3901 tacgcgaccg ggcgcgcaat aatgctgcgg gtttccatgc ggacttcggc aatggtgacg
     3961 tcaataacgt cagttacttt gtaaaccgtt tcacctttaa tttgtacagt gccgttttcc
     4021 tggctgcaaa ccagttcatc gcgcacagcg tgtaagaaag gtgccggaat aaaggcgata
     4081 gcgccgttat caaccaaacg cacacgcatg ccgccacggc tgatatcgac aatttccgcc
     4141 gcgaaacggg tgccggtccc ggctttatct ttcaggaagc gtgcgtataa ccagtcgcca
     4201 acatcacgtt ctgccatccg gttgagacgg cgacgttcgg ccatttggac agtgatttca
     4261 tcctgcggac gcgtcgcagt ttcgcctttg ataaccgctt tcagcagacg gtggttgatc
     4321 atgtcgccat atttacggat cggcgaagtc caggtggcgt atgcttccag accgaggcca
     4381 aagtgaggac cgggttcagt gctaatttca gcaaatgact ggaagcgacg aatgcggctg
     4441 tcgaggaaac cagttggttg cgcatccagt tcacgacgca gtttgcagaa accgtccagc
     4501 gtgagtactt cttcggcatc gacatgcaga ccgtgcgttt tcagcaacgc tgccagcgcg
     4561 tcggcattcg ccggatcaaa gcccatatgc acgttataga tgccaaaacc gagcttatcg
     4621 cgcagtacgc gggccgcaca aatgttagcg gcaatcatcg cttcttcgac gatacggttg
     4681 gcaatgcgac gaggctcggc gacgatatcc agcacttcac ctttttcacc gagaataaag
     4741 cggtaatccg ggcgatcttt aaacactagt gcgtggttat gacgccactc gccgcggcgt
     4801 tggcaaattt gcgctagcaa acggacttgt tcggcaattg cttcactttc aggcttccag
     4861 tcaccggtat tttccagcca gtcagaaacc tggtcataca ccagcttcgc tttggattcg
     4921 atggtggcgg caaagaattc gatattatct tcaatggtgc catcagcgga gagcgtcatg
     4981 cggcatgcca gtaccgggcg gacttcattg gcgcgcagtg agcagagatc gtcagaaagc
     5041 tcgcgaggca gcatagggat gttgaagcca ggcagatagt tggtgaatgc gcgaattttc
     5101 gcggctttgt ccagcttgct gccttcagca atccacgcgg ttggatcggc aatcgccaca
     5161 atcagctgaa gtttgtcatc cggcagcgcc ttagcgaaaa gggcgtcatc catatcttca
     5221 gtgctggcac tgtcgatggt gacaaaatcc agcgaggtca gatcttcacg aaccagacct
     5281 tcatcgagca tttcggtagc gacgccgtct ggtgcttctt tttccagatt atggcgcgca
     5341 agggtaaccc accacggtac aaagtggtcg tcgccaaaag tgatgtattg tgtcagttca
     5401 gcatagaaag aacgatcgcc tttcagcgga tgacggcgca tttcggcaac cgcccagtcg
     5461 ccttctttaa actcgtggtt caggccacgg gctgcgcggc aaggaatggc gtcttttaaa
     5521 agtggatgat caggaacgat ggtcagacgg tcatttttgc cctgaacttt acccacgaaa
     5581 cgagtcagga acggttcaac cagttcttct ggctctgcgg attcacgttc tttttcactg
     5641 tggatcaccg cgataattcg gtcgccatgc atgacttttt tcatctgcgg cggcggaatg
     5701 aaataacttt tttgtgcgtc gacttccagg aagccaaagc ctttttctgt ggcttttacc
     5761 accccttcag cgcgtggcgt ctgggaatgc agttgctgtt taagctgcgc tagcagcggg
     5821 ttgtcctgaa acataattgt ctattttggt ggccattaga gcggctgaca gttttacgcg
     5881 aatctgtctg acgcggcaag gttaatatgt ctcacccaac gcgattttta agcgatttat
     5941 ccagccacac agcccgctcc ataccagcag attaataatc tgcgttgatg atttttcgtg
     6001 ttcgagtaaa gggctgaact gggcggcgct aaagcgatcg ggtgctcggg tcagccattg
     6061 tatcgcctga acggtagcgc gctcaacaga atgacggtca gcccatcggt taatttcatt
     6121 ttcgcccttg cgcagaaggg tgctgatttt tttatattca tttgatgaat ccatacaggt
     6181 ggaaaaacaa gcaggactgc catttatacg cgcgcataac aacgccgcaa gctgattatc
     6241 cggctgtgta ttgacccgtg tgctgtttaa caacttttcg agaagggaaa gtgaaatagg
     6301 gtggagagag tgtgtttcgg ccagttggca aagttctgca gttgaacttt ttgtgtgctc
     6361 ttcaggtagt ttttccacct catagctgct tcgccagcgt attgattcat cagcaaacag
     6421 tgacgcgtcg gcgtagtttt gtatcgccag cccgggtaac cagcgtaccg gatgcccgag
     6481 ataagcctga aatgtcgcaa ttgtccgcgc ctgaaagcca ataaaaccaa tgatttggtt
     6541 aatgatcaca cagtcccaga cagtaagacc gacgtcattc agatgctggc gcgatcggtt
     6601 gtcgataata gcaggcgagc tagccagttg gcgggcatat tgcgtgattt gtgccaaccg
     6661 atgattactt tccctggagg aatcaggtcc ggggagcggc gtaagtcgcg ccgaatagta
     6721 gttacataac cgctggacgc cgcaaacctg agcaaccgtc agggccgtcg acaagcgttc
     6781 ataagcggtg aaggtttgtg aacgggtcac ggtaagctgg tcagggaaca actccactgc
     6841 aagaacgcgc gcagggacca gccatgaaag gaagggggcc agcgtctctt catcaaggat
     6901 cacgtcgagc agaaagggat cgctgacctt tgcggcttcc gggaccagag gcagaacgtc
     6961 atactccgta gtactggatt gcgtttcatg ataccagtgg cttttgccgg tgatgtggcg
     7021 ttgttccatg ggcgctcctt ggtcgtaaag gaaatcgtta tcctgacgca aggcgggaag
     7081 gggagaaaga cggatcggcg ataacaaata tcagaaaggt ataacagaga taacgggcgg
     7141 cagaacgccg cccatcttta ccaacagaac gattatttca gttcgagttc gttcattgca
     7201 gcgatgctga aaccgccgtc aacgtggact acttcaccgg agataccggc agagagatcg
     7261 gagcacagga atgccgcaga gttacccaca tcttcaatag taacggtacg gcgaatcggg
     7321 gtaacggctt cgcaatgagc cagcattttg cggaagtctt tgataccgga ggccgccaga
     7381 gtacggatcg gaccagcaga gatggcgtta acacgcacac cttccggacc catcgcgttc
     7441 gccatatagc gcacgttcgc ttccagagac gcttttgcca gacccataac gttgtagttc
     7501 gggatagcgc gctcagcgcc aaggtaggaa agggtcagca gggcagaacc cggattcagc
     7561 atggagcggc aagcttttgc cattgcaacg aagctgtagg agctgatgtc gtgggcaatt
     7621 ttgaagcctt cacgggtaac ggcgttaaca tagtcaccat ccagctgatc gccaggtgca
     7681 aaaccaatag agtgtacgaa accgtcaaat ttcggccaaa ctttccccag ttcagcgaac
     7741 atggtgtcga tgctggcatc ttctgcaaca tcgcactgca gaacgatgtc agaacccaat
     7801 tgagcggcaa attcttctac gcggcctttc agtttgtcgt tctggtaggt gaatgccagt
     7861 tcagctcctt cgcggtgcat cgcctgagcg ataccgtagg cgatggacag tttgctggca
     7921 acaccggtta ccagaatgcg cttaccggaa agaaaaccca tagctttaat ccttattgtt
     7981 gatgcttgtt gtgcctgaaa atcaggcgat tcgtcgtttt agtaaacagt acgaacagat
     8041 aaacggttat tataatcaac ctggctgtga gtagctatag ttgccaggtc cgaccggagc
     8101 aggctgcggc agggggggcg cttttcccct taccctaacc ctctccccag aggggcgagg
     8161 ggaccatatt gtgcaaatat tgttacccca gcaacaaaca ggctcataca gcccctaacc
     8221 cttttatggc gatggctggg atggttcaga ccttgccgaa tattctccag cactgtctcc
     8281 atgttttcac cacaacagct attcggctcg gtcggccccc tcgccctttc agggtgaggg
     8341 tgaccggcgg ttcagttcct gcagaataat ctccagcacc gcctcctcat tacaatcaat
     8401 ctcgttattc cagaaacgca gcacggtcca gccctgcgac tcaagccagc tagtgcgcct
     8461 ggtatcatag gcaactgcca aatcatgctg cccaccatcc agctcaacga ctacgcgcgc
     8521 cgagcagcaa gcaaaatcga gaatgtagct ccccaccgga tgttgacggc gaaatttgaa
     8581 atcaccaaaa cgtcggctgc gaagatatcg ccagagcttt cgttcctgca aagtgagatt
     8641 gcgtcttaaa tcacgcgcat ttgattttat tttatccatc acctcattct gacaagattt
     8701 aatctcttgt cagaatgagg tgaataatct ggaaggagga ttcagaaaat tagcgatctt
     8761 tacgccacgc atccgctgtc aatgcttcgc caaagtgccc ggcaatcagc cgtttggtga
     8821 gttcatgcag cggcgatgcc agcacatccg cggtgctgcc tcgctcgaca acctcgccct
     8881 gatgcatcac cagcacctgg tcgctaatgt gcttcatcat tccgatatgc tgggtaacat
     8941 aaatatacga aatgccctgt ttttcctgta actccagcat cagattaatc aactgcgaac
     9001 gcatcgacat atccagtgaa gcgagggctt catcggcaat aatgactttt gggcgcaata
     9061 tcagcgcgcg cgccagaccc agacgctgtt tttgtccggg tgccaacata tgcggatagt
     9121 aactgacgtg atccggtagc agcccaacca tacgcatcgt ttcaataatc tgtttgcgac
     9181 gctgttccgg ttccaggtcg gtgttcaggc gcagtggaaa atccagaatt tgtgagatac
     9241 gttgacgggg attcaacgag gtcgaaggat cctgaaaaat catgcgaata cgctgactac
     9301 ggaaggaata atcgccaaaa tgcagcggat gatcgtcaat caataactcg ccgctggtag
     9361 gttctatcat tcccgccagc atttttgcca gtgtggattt acccgaacca ttctcgccaa
     9421 taatcgccag tgtctggcct tcacgtagcg taaagctcaa gggttttacc gcttctacgg
     9481 tctgacgacg aaaccaaccg gtccggtagc ggaacgtttt acttaaatta cgcacttcaa
     9541 gcagcgtttc gatcatctca ctctttctcc atgttcagcg ggaaatgaca ggcatagaga
     9601 tgatttttcg cccccgtcaa acgtggcgtc acaatgcatt ctcgttgtgc atacgggcaa
     9661 cgtggcccca gacgacaacc aatcggtaac tgttccagca atgggatagc gccgggtaga
     9721 gtattgaggc gacttttatg cggcatcgcg ctgccgaagt ctggtatcgc gcggatcagc
     9781 gcctgggtat aaggatgatg tggcatcgtc accaactcct tactcggcgc ggtttccact
     9841 gtttgaccgc agtaaagcac gttaatttta tccgcccatt ggctaagcat ttgtaagtca
     9901 tggctgataa gcaaaatagt ggtattgctg ttttggttga gacgcgtcag caggcgaaag
     9961 atttgcgcct gggttgttgg ctccattgag tttgtcggtt cgtcagcaat cagcagacgc
    10021 ggttgattcg ccagtgcaat ggctatcatc actttctgac attcaccttc ggtcaactca
    10081 tagggaaaac tgcgcatcgc atctttgtga tctttaatcc ccacgcggtg cagcagttca
    10141 atggcacggc gtttgcgcca gccaaaacgc tgccaccaac ggcctttata ggtccaggct
    10201 gggatgtttt gcatcaactg gcgacccaca cgttctgaag ggtcaagaca cgactgcggt
    10261 tcctggaaaa tcatcgacac gttatggcca accagtttgc gccgttcgcg tgcggagaga
    10321 cgcagcaaat cgatatcatc aaaacgcata cggtcagcag taacacgcca gttatcttta
    10381 ttcaccccac aaatcgcttt cgcaatcaaa ctcttgccgg aaccggattc accaacaaga
    10441 ccgcggattt caccttcggt taaggtcata cttacccggt cgacggcttt aacccactca
    10501 tcaccggttt taaattcaat ggtcaggtta cgaatatcga gtaatggcat tattccaccc
    10561 ccgcaataat cgcacgacga acgccatcgc caaggaggtt aaccaacaac acgctaatca
    10621 taattgccgc acctggcagc atgacagtcc acggggcgac ataaatcagt tccagcgcat
    10681 caccgagcat cgctccccat tcaggcgagg ggagttgtgc gccgagatcg agaaagccca
    10741 gcgcggcgat atcgagaatt gccatcgaca gtgcgcgggt gatctcggtg accagcccgg
    10801 cggtgatgtt tggcatcaca gcaaaccaga gaatattcag cgttgatgcg ccatccagac
    10861 gggcggcgat aacgtactct ttttccagtt cgtcatgcac catgctgtaa atcgaacgta
    10921 ccatacgcgg cagcaatgcc agccagacag caaacatagc gtgggacaaa ctcggtccgg
    10981 caaacgctac aacgataatt gccaacagca gcgaagggat cgccagtaat gtatccagaa
    11041 tatggttaag caccgctgag cggaggccgt gtgtcgcccc ggcgaaggtg cccagtacca
    11101 ggccacagat cgtcgcggca agcgtcacca caaatgcgcc acccacggtt ggcgcagctc
    11161 cgctcagtaa acggcttaaa acatcacgtc cgaggtcgtc agtccccagg aagaaagaaa
    11221 cttcgccata gcgcgaccat gatggcggca ataattgata accgagaaat tgctggtcga
    11281 tgccgtaagg cgcaaaccag ccgccaaaaa tacacagtac agccagtccc gcgcagccgt
    11341 acaggccgac catcgcagag gcgtcactat aaaatttgcg ccaggcggta cgcagcgtgc
    11401 ccggcgggcg cttttcgctg tatacgctat cgtaaggcat accattcctt atgtttcaga
    11461 gggttagcca tggcacccaa aatatcagaa atcacgttaa caataatgac cagtgagcca
    11521 cacaccatca ctccggcgga aatggctgcg taatcctgct ggcggatggc gttaattaac
    11581 cagcgcccta aacccggcca gctaaaaacc atttcggtga tcatcgccag cgtcagcatg
    11641 gtggaaaact gtaagcccag gcgaggaata accggcggta acgcgttatg cagaacgtgg
    11701 cgacgcaaaa tagtaaagcg cgacaaaccg cgggtcgccg ccgcttttac atagttctgg
    11761 tcatacactt caatagtgct gatacgcatc aggcggatca cttcagttgt tggcgcaacc
    11821 gacagggtga tcacgggcaa tatcatatgg cggattgcgc tcatgatcat ttcatcccgc
    11881 catggtgagt ccgaaagcca ggcatcaatc aacgcaaaac cggtaatcgg tttcacttcg
    11941 tagagcagat cgaaacgccc tgaaacgggc aaccaaccta gcgtgagtga acaaaacagt
    12001 gtcaacagaa gcgccagcca gaacaccggg attgaaaagc ccagtaaggc gatggcgttg
    12061 atcaagttat cctgccactt atggcgcgta atcccggcaa tcatccccac ggggatgcca
    12121 acaatcagcg caaaaccgaa ggcgaggatg cacagttcta tcgtcgccgg gaagacctct
    12181 tttaactgtt cggcaattgg ttgaccgtta atgctggaaa caccaaaatc ccagtggatc
    12241 aggccattaa accagaacac ccaggcattc cacagtgacg cgccttgtaa cggcgcgtgt
    12301 ggagtgaaat agctcaagct gaagccaacg aaggtcagca ggaacaaggt gacaatcaat
    12361 aacaaaatgc ggcgtaaggt gaagataatc atggtttttt cacctcatcc tgtttctcgc
    12421 gatacacccc agcaaaggag gcgttaccaa acgggctaag taccagacct ttgatatcgt
    12481 accgataggc ctgcaaacgc aatgacgacg ccagcggcag aataggtaat tcctgcgcca
    12541 gaatactctg cgcttcgtca taggcttcaa tacgcgctgc cagctgctgc gaggagagcg
    12601 ccttacgcaa cacgctgtcg aatttcggat cgcaccagtg ggcgaggttg gtctgtgaat
    12661 gaattgccgc gcagctcagt aacggacgga agaaactgtc cgggtcgtta ctgtccgtcg
    12721 cccaaccgga taacgtcaga tcatggctca tatccatcaa ccgcgcctcc tgaaagcgac
    12781 cttctaccgg cacaatcacc acttttacgc caacctgcgc catatccgcc tgaatcagtt
    12841 cggcagtttt cagtggactg gggttccacg cctgcgaacg tgtgggcacc cacagtttca
    12901 gcgttaagtt ttccagcccc aacgccttca actgttcgcg cgatttcgcc ggattgtatt
    12961 cagtaatttt agcctcgttg tcataggccc acgaggcgcg cggtaaaata gaggccgccg
    13021 tttcagccgt accataatag atggattgca tcaggcgctg gttattaatc gccagtgcca
    13081 gtgcatggcg gacagcggga ttatttagcg gcggtttggc ggtgttaaat gccagatagg
    13141 cgacgttcat cccaggacgc agcgttaaac gcaagcgcgg gtcgtcacgc aaaatggata
    13201 gctggctggc agcaggccag gccagaacgt cgcattcccc ggtcaggagt ttcgacagac
    13261 gtccggtgcc gccggagcct aaatccacca ctacctgcgg cattaacggt ttaccgcgcc
    13321 agaagtcatc atgacgttgt aggcgaataa attgcccggc gcggtattcc gacaactgat
    13381 acggaccggt gccgaccggt tgacggtcga gttgctcctg gcgatcttct ttctctaact
    13441 tccgggcata ttctgccgac atgaccgaag cataatgggt tgcgaggtgc cacaaaaaag
    13501 aagcatccgg ctgggcgagt cggaactcaa cggtatgatt atccagtttg cggacgcttt
    13561 tcacgttatc ggcaaattgc aggctgtcga agtaggggaa gttgctgccg ttgacgttat
    13621 gccacgggtt gttgcggtca aaaattcgct ggaaggtaaa caccacatcg tcggcattca
    13681 ttttacgagt gggagtaaac cagtcggttt tttgaaacgg aacatcgcga cgcaggtgga
    13741 agcgataggt cgcgccgttg tcgagtactt cccagctttc ggcaagttcc ggcatcaggc
    13801 gataggtata gggatcgaca tccagcagtc gatcataaaa ctgggcggca agggtatcga
    13861 caattaaccc actgctcgct ttggatgggt taaaggtgtt gacttgcccg ctgacgcaat
    13921 agacaaaacc gctgtcgcgg atatcagcat gcggggggga ttcaggcgcg gcgattgcct
    13981 gaccactcac aagtccagca atcaccaaaa gagacgataa tacctggcgc ataatattaa
    14041 gggattttat gtaaagaggc tatcttacta atatttaatg acatttgcca ttaccgtttg
    14101 tgttcagggg tcgtaatgag ggccgtgtgg ttggtcgttg aacaggtggc gtcgccatct
    14161 attcaacatt cgtacccgtg atttctctat aactataact cacagaacaa cttagcgagg
    14221 agcagagcgg tacgatcgtg agaaacccat taaagcacgc tgcagtgcgt gtcattgtta
    14281 gccggatgcg gcgtgaacgc tttatccgga caacgatact gaccgatcgt ctgcaattaa
    14341 ataattactc attaccccat tgattcaaaa actctgcgat ctcatcaatg cgtacgggat
    14401 taatccccgc ttcagcagcc atttcatgtt gggcttcttc gctgatctct tcattgttca
    14461 tcaaatgggt gagcagtaac tggaagtagt gggccagcgg tgtgcgttca gcctctgcga
    14521 caggctctgc gcattcggtc gcgtactcat cggcaatatc aaaatatttt agcgggatat
    14581 cgtggttcat tatttgcccc tggggttaat gcgctaagca taagggcgga tgatagcact
    14641 cgtgatccgg gtcatacatc tttttatagt taatggcgcg gcgcgttacc ctcaggcagg
    14701 ataatgcgcc gcgcatccgg ctattacgtt tcgctcaccg gcgttctgtc accttcgtac
    14761 agcggcactt tgcgatagcg gcggatcaga taccacaaat atgcgccgcc taacgaagcc
    14821 cataccagac cgagtgtcag tgacgttgac tctaggttaa cccacagcac acccaccgtc
    14881 agcgcgccaa ccagcggcat cagcaaatag tggaagtgat ccttccagct tttattcatt
    14941 cctttacgcc gccagaaatg attaaacacc gacagattca cgaaggtaaa cgccaccaga
    15001 gcaccgaagt taatcagtgc tgtcgcggtg actaaatcaa agaacagcgc cgacagcgcg
    15061 acaatcccga ccataatgac gttgagtgcc ggagtccgcc attttgggtg cacatagcca
    15121 aacacgcgct ccggaaacac attgtcacgc cccatcacat acagcagacg cgacacgctg
    15181 gcatgtgaag ccaggccaga cgctaatgtg ttcacaaacg tggtgcagag gaaaatcgac
    15241 tgaaagagct tgccgccgac gtacagcgca atttcaggca gtgcggcgtc cgggtcttta
    15301 aagcggctga tatcgggaaa gaacagctgc ataaagaacg acgccgcgat aaagataacg
    15361 ccgccgtata ccgccgtcag gaagatggct ttcgggatca cgcgtgcggc gtccggcgtc
    15421 tcttccgaaa gcgtggtcac cgcatcgaaa ccgaggaacg agaaacagac aatcgtcgca
    15481 ccggtaataa tcgggatcag gtgcgcgttc tcgctgataa acggctgaag tgaccagacg
    15541 gtgccaacgc cttctccttt atgcagtccc tgaaccacca ggaagataaa caccaccatg
    15601 atggagattt gcaccagtac aaacagggta ttgaagttag cgaccaggtt gacgctcttc
    15661 agattcgcgg cggttaaaat ggcgacgaag gttaccaccc acacccacgg cggcacttcc
    15721 gggaagaggg cggagagata gattttcgcc aacaaaacgt tgatcattgg caaaaagaga
    15781 taatccagca gtgatgacca gccgaccata aatccgacgt gcgggttaat cgacttttgc
    15841 gcgtaggtat aggccgaacc ggcctccgga aactggtgaa ccagtttgcc gtagctgata
    15901 gcggtaaaca gcacgcccgc cagcgccagc aaataggacg ccggaacgtg accgtcgcta
    15961 atgccggaga caataccaaa agtatcaaat acggtcatcg gcgtgagata ggccagaccc
    16021 atcattacca cctgccacaa tttcagtgat tttcgcagac gggttttgcc gggttgcgca
    16081 gcaatattca gtggtgaatt aatagccata attgctgcct cccctgcgta ataaggtttg
    16141 cggtcgtgtc gacctgaaat caaacctgtt tagcaaggga agcaaagggg gcggagtcca
    16201 gtcagtagca ggaggggcgc atacgcagcc tccctcgcca tgcggggatt taatcatcgg
    16261 catcatctca tttccttgtc acagtcttaa tcgttaacgg aagcccgttg ccgcctgcgc
    16321 tccataacgc gacatccggg aaatgtctgg attaccagca aatgtcaggc ctggctccgc
    16381 tcaggccgat gaaacaaccc cgcaagggga attacgcgtt tttcaacatc cactcaattt
    16441 ctgtttctgt gatgaggcgc tcaaactgca acagctcatc atttttacag gcgtgataca
    16501 catggcaaaa ccgttcgcct aaatagcggc gcaggtgatc attctcgata aactcaccca
    16561 ggcatcgctc tggcgaatcg ggaagggtaa gccttcctgt tccagcccgt tgccttcgac
    16621 ttcttcctgc aacggcagct cgttatcaag gccatgcaaa ataccggcaa aaatcgctgc
    16681 catcaccaga tatgggttgg catcggcacc cgccacgtga tattccacgc ggtgattatg
    16741 acggtcaccg cacggaatac gcagggcgac ggtgcggttg ttatggcccc acgacgcctg
    16801 cgtcggcaca tacattcccg gctggaagcg gcgatacgag ttcacgtttg gtgccagcaa
    16861 cgccatcgac gacggcatca ggtcaatcat cccggcgagc atctttttca gcagcggcga
    16921 atcttcgcct tctgtgtcag aaagcacatt ctcgccacga ttattttgca tactgatatg
    16981 gatatgcatc ccgctgcccg cgtgctcttc atacggcttc gccataaaag tggcgtgcat
    17041 cttatgcttt tctgccatca gacgcaccag gcgttttagt gccagcgcat catcgcaggc
    17101 ttccagcacg ttatcagtat ggtaaaggtt gatttcaaac tgacccggcg aggcctcagc
    17161 gaccgcgcca tctgccggaa tcagctgcaa ctgcgccagt tcatcaatat cattgagcac
    17221 gtcggcgaag tggttgaggt tatcaacgga gtaaacctgg ctttgtgtat tgcggtcatc
    17281 ggtgccaggc gcgcagggcg gttgcagatc cccttcagcg tcgcgctggc gatccagtaa
    17341 atagaactcc agctctaccg ctacaaccgg gaacaatccg cgctggcgca gctgctgcca
    17401 caggcggtta agaacgttcc gcggctcaac gtcaaaggga gcgccatctt catcgaccat
    17461 ggtcaggagc atctgatcga taaactctgg atcggcggca gaaggagtta aggaaccgag
    17521 aacaggaaca caggtacgat ccggctcgcc catttcctga cccagacctg cttcttctac
    17581 tacgttgccc agaatatcca ttgcaaatac cgaggccggg aaataacacc ctttctcgag
    17641 cttcttcagg cttgaaacag ggatacgttt tccacggaag caaccattca aatcagtaag
    17701 taaaacatca acatattgcg tattcgggta gcgctccagg tagcgtttca cttcctgcgt
    17761 aaaggcgcta ccccgcctct cttctgactg ctgaacaaag ttctctactt caacgatatt
    17821 ggtttccatg attcttcgcc tttggtttgt tttccgctcg ttatcaaagc gtaaaatata
    17881 atgaccatca ttcgaatctg tatgcaaacc aaatgtttgt caaatgttaa atttagtttg
    17941 caaaaatgaa aatccactgc tagattgaaa aaatattgaa cataaaggtc atttaaagcg
    18001 cagtaatggc gataatttag tccactttgt gagattgagc atggaaaata taatgaacaa
    18061 tccggttatc ggtgtcgtaa tgtgcaggaa caggcttaag ggtcatgcga cccagactct
    18121 gcaagaaaag tacctgaatg ccatcatcca cgcaggcggc ttgccaattg cgctaccaca
    18181 tgcgctggcg gaaccgtcgt tacttgaaca acttttgccg aaactcgatg gcatttatct
    18241 tcctagtagt cccagcaatg tgcagccgca cctatatggt gaaaacggcg atgagcctga
    18301 cgccgatccc gggcgtgatc ttctgagcat ggcgataatt aatgccgcac tcgaaaggcg
    18361 catccccatt ttcgccatct gccggggttt acaagaactg gtggtggcaa ccggtggttc
    18421 gttgcatcgc aagctgtgcg aacagcctga attgctggag catcgggaag atcccgaact
    18481 gccggtggaa cagcaatatg caccgtcgca tgaagttcag gttgaagagg ggggattact
    18541 gtctgcgttg ttacctgaat gtagcaactt ttgggtaaac tctctacatg gacaaggggc
    18601 gaaggtcgtt agcccacggt tgcgtgttga agctcgctcg ccggatggct tagttgaggc
    18661 ggttagcgtc atcaatcatc cttttgcgct gggcgtacag tggcacccgg aatggaacag
    18721 tagcgagtac gcgctttcgc gtatattgtt cgagggcttt atcaccgctt ggcagcacca
    18781 tatcgctgaa aaacagcgac tctgaccact acagtttaag gaaatgcaaa tatgagtgat
    18841 gagggactgg cgccaggaaa acgcttgtcg gaaatccgcc agcagcaggg gctttcacaa
    18901 cgtcgtgccg ccgaactctc cgggctgact cacagtgcta tcagtacgat agaacaagat
    18961 aaagtcagcc ctgccatcag tacgctgcaa aagctgctga aggtgtatgg tctgtcactc
    19021 tcggaattct tttccgagcc ggaaaaacct gatgagccgc aggtcgtcat taatcaggac
    19081 gacttaattg agatgggcag tcagggtgtg tcaatgaagc tggttcataa cggtaacccg
    19141 aatcgcacgc tggcgatgat ctttgaaacg taccagccgg gcacaaccac tggggaaaga
    19201 attaagcatc agggtgagga aataggcaca gtactggaag gtgaaattgt tctgacgatt
    19261 aatggtcagg attaccacct cgtcgcgggg caaagttatg ccattaatac cggcatcccg
    19321 cacagtttca gtaatacgtc ggcaggtatt tgccgaatta tcagcgccca tacgcccacc
    19381 acgttttaat ctttttgttc tgtaagccgg gtaagcgcaa tgcgcccggc aatccataaa
    19441 gaatgtcgcc agaaataaac gggcatgcag cccggggatc tctgcgccct gacgttcaca
    19501 aactgcatat atctgataga cgtgaaacag gagtcataat gaattttcat catctggctt
    19561 actggcagga taaagcgtta agtctcgcca ttgaaaaccg cttatttatt aacggtgaat
    19621 atactgctgc ggcggaaaat gaaacctttg aaaccgttga tccggtcacc caggcaccgc
    19681 tggcgaaaat tgcccgcggc aagagcgtcg atatcgaccg tgcggtgagc gcagctcgcg
    19741 gcgtatttga acgcggcgac tggtcactct cttctccggc taaacgtaaa gcggtactga
    19801 ataaactcgc cgatttaatg gaagcccacg ccgaagagct ggcactgctg gaaactctcg
    19861 acaccggcaa accgattcgt cacagtctgc gtgatgatat tcccggcgcg gcgcgcgcca
    19921 ttcgctggta cgccgaagcg atcgacaaag tgtatggcga agtggcgacc accagtagcc
    19981 atgagctggc gatgatcgtg cgtgaaccgg tcggcgtgat tgccgccatc gtgccgtgga
    20041 acttcccgct gttgctgact tgctggaaac tcggcccggc gctggcggcg ggaaacagcg
    20101 tgattctaaa accgtctgaa aaatcaccgc tcagtgcgat tcgtctcgcg gggctggcga
    20161 aagaagcagg cttgccggat ggtgtgttga acgtggtgac gggttttggt catgaagcgg
    20221 ggcaggcgct ttcgcgtcat aacgatatcg atgccatcgc ctttaccggc tccacgcgta
    20281 cgggtaaaca actgctgaaa gacgcgggtg atagcaacat gaaacgtgtc tggctggaag
    20341 cgggcggcaa aagcgccaac atcgttttcg ccgattgtcc ggatctgcaa aaagcggcaa
    20401 gcgccaccgc cgcaggcatc ttctacaacc agggccaggt gtgcatcgcc ggaacgcgtt
    20461 tgttactgga agagagcatc gccgatgaat tcttagccct gttaaaacag caggcgcaaa
    20521 actggcagcc gggccatcca cttgatcccg caaccaccat gggcacctta atcgacagcg
    20581 ctcacgctga ctcggtacac acctttattc gtgaaggcga aggcaagggg caactgctgc
    20641 tggatggtcg caacgccggg ctggcagcgg ccatcggccc gaccatcttc gtggatgtgg
    20701 acccgaatgc gtccttaagc cgcgaagaga ttttcggtcc ggtgctggtg gtcacccgtt
    20761 tcacatcaga agatcaggcg ctgcagcttg ccaacgacag ccagtacggc cttggcgcgg
    20821 cggtatggac gcgcgacctc tcccgcgcgc accgcatgag ccgacgcctg aaagccggtt
    20881 ccgtcttcgt caataactac aacgacggcg atatgaccgt gccgtttggc ggctataagc
    20941 agagcggcaa cggtcgcgac aaatccctgc atgcccttga aaaattcact gaactgaaaa
    21001 ccatctggat aagcctggag gcctgaaatg accgaacata ccagcagtta ctacgccgcc
    21061 agtgcgaata aatatgcacc attcgacaca ctgaatgagt caatcacctg cgacgtttgc
    21121 gtggttggcg gcggctatac cgggctctcc tccgcgctgc atctggcgga agcgggcttt
    21181 gacgtagtgg ttctcgaagc ctcacgcatc ggctttggcg caagcgggcg caatggcggg
    21241 cagcttgtga actcctacag ccgcgacatc gacgtgatcg aaaaaagcta cggcatggac
    21301 accgcccgta tgctcggcag catgatgttc gagggtggcg agatcatccg cgaacgtatc
    21361 aaacgttatc agattgactg cgactaccgc cccggcggtc tgtttgtggc gatgaatgat
    21421 aaacaactcg ccacactcga agagcagaaa gagaactggg aacgctacgg caataaacag
    21481 ctggagctgc tggacgccaa cgctattcgc cgcgaggtcg ccagcgatcg ctacactggt
    21541 gcgctgctgg atcacagcgg tgggcatatt cacccgctaa acctcgccat tggtgaagcc
    21601 gatgccatcc gcctgaacgg cgggcgcgtg tatgagcttt ccgccgtgac gcagatccag
    21661 cacaccacgc cagccgtggt tcggactgcc aatggtcagg tgacggcgaa gtatgtgatt
    21721 gtcgccggga atgcgtatct gggcgataaa gtagagccgg aactagcgaa acgcagcatg
    21781 ccgtgcggca cgcaggtgat caccaccgaa cggctgtcgg aagatttagc ccgttcgctg
    21841 atcccgaaaa actactgtgt ggaagactgt aactatctgc ttgattacta ccgtcttacc
    21901 gccgacaacc gcctgctgta cggcgggggt gtagtgtatg gtgcacgcaa cccggatgac
    21961 gttgagcgtc ttgtggtacc gaaactacta aaaaccttcc ctcagttgaa gggcgtgaaa
    22021 attgattacc gctggacggg caacttcctg ctgaccttgt cgcgcatgcc gcagttcggc
    22081 cgccttgata ccaacatcta ttacatgcag ggttacagcg gccacggcgt cacctgtacc
    22141 catcttgccg ggcgtttgat tgctgaactg ctgcgcggtg acgccgagcg tttcgatgcc
    22201 ttcgccaatc tgccgcatta cccgttcccc ggcgggcgca cgctgcgcgt gccatttacc
    22261 gcgatgggcg cggcttatta cagcctgcgc gatcgtctgg gcgtttaatt tccgattaac
    22321 agtgaagagt caaaaggtgt gaaacatgag caacaatgaa ttccatcagc gtcgtctttc
    22381 tgctacccca cgcggggttg gcgtgatgtg taacttcttc gcccagtcgg cagaaaacgc
    22441 tacgctgaag gacgtagagg gcaacgaata catcgatttc gccgcaggca ttgcggtgct
    22501 gaataccggg catcgccacc ctgatctggt cgcggcggtg gagcagcaat tgcaacagtt
    22561 tacccacacg gcgtatcaga ttgtgccgta cgaaagctac gtcacgctgg cggagaaaat
    22621 taacgccctt gccccagtga gcgggcaggc taaaaccgca ttcttcacta ccggcgcgga
    22681 agcggtggaa aacgcggtga aaattgctcg cgcccatacc ggacgacctg gcgtgattgc
    22741 gtttagcggt ggcttccacg gtcgtacata tatgaccatg gcgctgaccg gaaaagttgc
    22801 gccgtacaaa atcggcttcg gcccgttccc tggttcggtg tatcacgtac cttatccgtc
    22861 agatttacac ggcatttcaa cacaggactc cctcgacgcc atcgaacgct tgtttaaatc
    22921 agacatcgaa gcgaagcagg tcgcggccat tattttcgaa ccggtgcagg gcgagggcgg
    22981 tttcaacgtt gcgccaaaag agctggttgc cgccattcgc cgcctgtgcg acgagcacgg
    23041 tattgtgatg attgccgatg aagtgcaaag cggctttgcg cgtaccggta aactgtttgc
    23101 catggatcat tacgccgata agccggactt aatgacgatg gcgaaaagcc tcgcgggcgg
    23161 gatgccgctt tcgggcgtgg tcggtaacgc gaatattatg gacgcgcccg cgccgggcgg
    23221 gttgggtggc acctacgccg ggaacccgct ggcggtggct gccgcgcacg cggtgctcaa
    23281 tattatcgac aaagaatcac tctgcgaacg cgcgaatcaa ctgggccagc gtctcaaaaa
    23341 cacgttgatt gatgccaaag aaagcgttcc ggccattgct gcggtacgcg gcctggggtc
    23401 gatgattgcg gcagagttta acgatccgca aacgggcgag ccgtcagcgg cgattgcaca
    23461 gaaaatccag caacgcgcgc tggcgcaggg gctgctcctg ctgacctgtg gcgcatacgg
    23521 caacgtgatt cgcttcctgt atccgctgac catcccggat gcgcaattcg atgcggcaat
    23581 gaaaattttg caggatgcgc tgagcgatta atagcccaac gcaataatgt ctgatgcgct
    23641 gcgcttatca ggcctgcaaa cgacgtattg attatgtatg ccgaataaag cattcacgcc
    23701 gcatccggca agttgtattg ctcaacttcg ctaaatctgg tgctttttca acaacgcgcg
    23761 gaactgatga taagttaacc ccagtaattc agccgcgcgc ttctggttat atttcccctg
    23821 ttgcaaactg agttgcagca actctttttc ctgctgcatc tgaaactcgc gtaaatccag
    23881 cggcagcgtc ggaagcgagg tattttctga aacggcgatg gcttcttcag gtggacgccg
    23941 tttaaaggga tcaataatga tgtcatcaag cggataatcg ctggtgccgt ggcgatacac
    24001 tgaacgttcc accacgtttt tcaattcacg aatatttccc ggccaacgat aattcagcaa
    24061 tgtttctctg gcgcgctccg taaaccccgg gaacagaggc agcttgattt cccgacacat
    24121 ctgaatggca aagtgttctg ccatcagcat aatgtcgctt tcgcgctcgc gcaggggcgg
    24181 cagttgcact acatcgaaag ccagccggtc gagcaggtca gcgcgaaaag taccttcatt
    24241 gaccatcgcc gggagatcgg cattcgtcgc gcataccaac cgcacattca cctgcaacgg
    24301 ctggctaccg ccaacgcgct ccagttcacc gtactcaatc acacgcaata atttttcctg
    24361 caccatcatc ggtgccgtag cgagttcatc aagaaatagc gtaccgccgt cggcacgttc
    24421 aaatctccca ggatgacgtt tttgcgcacc ggtaaacgcc cccgcttcgt gaccaaacag
    24481 ttcggaatcc tgcagatttt catttaacgc cgcgcagtta agggaaataa acggcccttg
    24541 ccaacgggag gagagataat gcaggcggct ggcaatcaac tctttaccgg tgccgcgttc
    24601 gccgatgatg agcaccggtt tgtccagcgg tgcgagatgc gaaacctgtt ccagcacttc
    24661 gagaaagctg ttcgcctcac caagtaaatt atctttgtat tcggccatga tgaaattcgc
    24721 cacttgttag tgtaattcgc taattcatcc tggcatgttg ctgttgattc ttcaatcaga
    24781 tctttataaa tcaaaaagat aaaaaattgg cacgcaaatt gtattaacag ttcagcagga
    24841 caatcctgaa cgcagaaatc aagaggacaa cattatgggt attttttctc gctttgccga
    24901 catcgtgaat gccaacatca acgctctgtt agagaaagcg gaagatccac agaaactggt
    24961 gcgtctgatg atccaggaga tggaagatac actggttgaa gtacgttcta cttcggcgcg
    25021 tgcgttggca gaaaagaaac agctgactcg ccgtattgaa caagcgtcgg cgcgtgaagt
    25081 tgaatggcag gaaaaagccg aactggcgct gctgaaagag agagaggatc tggcacgtgc
    25141 agcgttaatt gaaaaacaga aactgaccga tctgattaag tccctggaac atgaagtgac
    25201 gctggtggac gatacgctgg cacgcatgaa gaaagagatt ggcgagctgg aaaacaaatt
    25261 gagcgaaaca cgcgctcgcc agcaggcatt gatgttacgt catcaggcgg caaactcgtc
    25321 gcgcgatgtg cgtcgtcagc tggacagtgg caaactggat gaagcaatgg ctcgtttcga
    25381 atctttcgaa cgtcgtattg accagatgga agcggaagca gaaagccaca gcttcggtaa
    25441 acaaaaatcg ctggacgatc agtttgccga actgaaagcc gatgatgcaa tcagcgaaca
    25501 actggcacaa ttaaaagcca aaatgaagca agacaatcaa taataacatc caggcggcgt
    25561 ccgaacgcgc cgccgctcat cgtctaagga gtacttatga gcgcgctatt tctggctatt
    25621 ccgttaacca tttttgtgct gtttgtttta ccgatctggt tatggctgca ttacagcaat
    25681 cgttctggtc gcagtgaatt gtcgcaaagt gagcagcagc gattagcgca actggttgat
    25741 gaagcaaaac ggatgcgcga acgtattcag gcgctggaat ctattcttga tgcagaacat
    25801 ccgaactgga gggatcgcta atggcgggca ttaatctcaa taaaaaatta tggcgtattc
    25861 cacagcaggg catggtccgc ggcgtctgcg ccgggattgc caactatttt gatgtaccgg
    25921 taaaactggt gcgtatcctg gtggtgctgt cgattttctt cggtctggcg ctgtttaccc
    25981 tggttgctta catcattttg tcatttgcgc ttgatccaat gccggacaac atggcctttg
    26041 gtgagcagct accttccagc agcgaattgc tggatgaagt cgaccgtgaa ctggcggcaa
    26101 gtgaaacgcg tttacgcgag atggaacgtt atgtcacttc cgatactttc acgttacgta
    26161 gccgtttccg tcaactgtga ggaaagttat gaatactcgc tggcaacagg ccgggcaaaa
    26221 ggtgaagctt ggtttcaaat tagcaggcaa gctggtactt cttacc
//
LOCUS       DADLXX010000045        25444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLXX010000045 DADLXX010000000
VERSION     DADLXX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.045"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            273..614
                     /locus_tag="JNX27_001879"
                     /pseudo
     CDS             273..614
                     /locus_tag="JNX27_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="JNX27_001880"
     CDS             complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="JNX27_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7883114.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            2626..3186
                     /locus_tag="JNX27_001881"
     CDS             2626..3186
                     /locus_tag="JNX27_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7883115.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            3361..3570
                     /gene="cspE"
                     /locus_tag="JNX27_001882"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             3361..3570
                     /gene="cspE"
                     /locus_tag="JNX27_001882"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7883116.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="JNX27_001883"
     CDS             complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="JNX27_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7883117.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            4100..4888
                     /locus_tag="JNX27_001884"
     CDS             4100..4888
                     /locus_tag="JNX27_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7883118.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTLGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            5017..5220
                     /gene="tatE"
                     /locus_tag="JNX27_001885"
     CDS             5017..5220
                     /gene="tatE"
                     /locus_tag="JNX27_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7883119.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="JNX27_001886"
     CDS             complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="JNX27_001886"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7883120.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(6495..7448)
                     /locus_tag="JNX27_001887"
     CDS             complement(6495..7448)
                     /locus_tag="JNX27_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7883121.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQSVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="JNX27_001888"
     CDS             complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="JNX27_001888"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7883122.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            complement(8449..8712)
                     /locus_tag="JNX27_001889"
     CDS             complement(8449..8712)
                     /locus_tag="JNX27_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7883123.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="JNX27_001890"
     CDS             complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="JNX27_001890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7883124.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="JNX27_001891"
     CDS             complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="JNX27_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7883125.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="JNX27_001892"
                     /gene_synonym="rodA"
     CDS             complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="JNX27_001892"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7883126.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="JNX27_001893"
     CDS             complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="JNX27_001893"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7883127.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="JNX27_001894"
     CDS             complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="JNX27_001894"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7883128.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="JNX27_001895"
                     /gene_synonym="rsfA"
     CDS             complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="JNX27_001895"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7883129.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            complement(15367..15978)
                     /locus_tag="JNX27_001896"
     CDS             complement(15367..15978)
                     /locus_tag="JNX27_001896"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY7883130.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="JNX27_001897"
     CDS             complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="JNX27_001897"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7883131.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            complement(16645..17676)
                     /gene="holA"
                     /locus_tag="JNX27_001898"
     CDS             complement(16645..17676)
                     /gene="holA"
                     /locus_tag="JNX27_001898"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7883132.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="JNX27_001899"
     CDS             complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="JNX27_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7883133.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="JNX27_001900"
     CDS             complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="JNX27_001900"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7883134.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            21089..21571
                     /locus_tag="JNX27_001901"
     CDS             21089..21571
                     /locus_tag="JNX27_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7883135.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTELDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            complement(21641..22624)
                     /locus_tag="JNX27_001902"
     CDS             complement(21641..22624)
                     /locus_tag="JNX27_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7883136.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            22782..23489
                     /locus_tag="JNX27_001903"
     CDS             22782..23489
                     /locus_tag="JNX27_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7883137.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            23486..24913
                     /locus_tag="JNX27_001904"
     CDS             23486..24913
                     /locus_tag="JNX27_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7883138.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(24923..25381)
                     /locus_tag="JNX27_001905"
     CDS             complement(24923..25381)
                     /locus_tag="JNX27_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7883139.1"
                     /translation="MVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHG
                     SDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQG
                     YAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT         6357 a         6253 c         6322 g         6512 t
ORIGIN      
        1 atgagctgat ttgtgatatg agtcatttgg tgacagcctg gttgttatcg gttttgaacg
       61 ttttttgaag ctatgattga aatgatttat agtgtttttg tttttcgttt tttcaatgaa
      121 acaaaagcaa ttttttacta tctttcgcgt ttcattggtt aacgcctaaa atttgtacta
      181 caggcttgca agccccacca aatctaatcc catcagatcc cgaataatcc aatgttctcc
      241 cggttttttc gtacttcaag tgaatcaata cactggggca aacgctaacc cgtttccgcc
      301 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
      361 ttaatgccta tgctcgcggt gaacccaaag acgaactgcc gaccggcatt gaccccttga
      421 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
      481 tggcgcaggc actgaccatc agccgcacca ctgccaggcg ttatcttgaa tattgcgcca
      541 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagacca caacgcatat
      601 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
      661 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
      721 tagcccaaca agcaccggca ccgaggtgcg ttttacgact tcaaatggtg agattttcgc
      781 catcccggca accgcaacga ctacgccaga aacgggtgat agggtacgac ccaggtttga
      841 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
      901 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
      961 cagcatggtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     1021 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     1081 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     1141 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     1201 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     1261 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg cgggggcagt
     1321 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     1381 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     1441 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcctgcgccg ccagcaccac
     1501 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagctgctgc
     1561 gccacgactg ataccaacgt ttaccatcac cggaaacagg gttgccatca gcaaaacacc
     1621 cagaccggtt gcggaagaga cggccagtga catcagacaa gcaacaaaat aggcggcaat
     1681 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     1741 atcattcgcg ccgatatggg tcatgtaagc ggcaaagcca cacagcatca taatcatcat
     1801 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc
     1861 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     1921 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     1981 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     2041 aatttttcct gtctccaggc ccaaagtaaa taataaaaac ttcctaaagc ttaaggaaaa
     2101 aatatgccca ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaga
     2161 cattgaagtt gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta
     2221 tcacataata atgtgtgtaa attgatggtg gattgatgca aatttgttaa ttgcaatggt
     2281 gttagctgcg gctgtgcgct caaaaaataa tctaatatga gcatgggttg acgatatata
     2341 ttatatactt cgttaagatg attgttgtat ctcgttaaaa aaaaataatt tttccttgat
     2401 tgcattttgt catcaaaaaa gacttggttt ttcttttttg actattccca tcgcagaaaa
     2461 cgacgcatca tctttaatcg atgcgcggaa atatttaact tgaacaagcg gaaataaata
     2521 gagcagctat tcagattatt ctttatgttg ggtctattaa ggttatgtta attgtagctt
     2581 tgctatgcta gtagtagatt tttgataaat gttttatggt cacaaatgaa cgtgagtaaa
     2641 tatgtcgcta tcttttcctt tgtttttatt cagttaatca gcgttggtaa agtttttgct
     2701 aacgcagatg agaggatgac aacgtttaga gaaaatattg cacaaacctg gcaacagcct
     2761 gaacattatg atttatatat tcctgccatc acctggcatg cacgtttcgc ttacgacaaa
     2821 gaaaaaaccg atcgctataa cgagcgaccg tggggtggcg gttttggcct gtcgcgttgg
     2881 gatgaaaaag gaaactggca tggcctgtat gccatggcat ttaaggactc gtggaacaaa
     2941 tgggaaccga ttgccggata cggatgggaa agtacctggc gaccgctggc ggatgaaaat
     3001 tttcatttag gtctgggatt caccgctggc gtaacggcac gcgataactg gaattacatc
     3061 cctctcccgg ttctactgcc attggcctcc gtgggttatg gcccagtgac ttttcagatg
     3121 acctacattc cgggtaccta caacaatggc aatgtgtact ttgcctggat gcgctttcag
     3181 ttttgaggca aatgaagttt tagtaacttc tttaaaatca ataactaaaa taagcaacat
     3241 caaaaataac gcgactttta tcactttttg gtaaagttgc actggacaaa gcgtaccaca
     3301 attggtgtac tgataaccga cacagcattt gtgtctattt ttcatgtaaa ggtaattttg
     3361 atgtctaaga ttaaaggtaa cgttaagtgg tttaatgagt ccaaaggatt cggtttcatt
     3421 actccggaag acggcagcaa agacgtgttc gtacacttct ctgcaatcca gactaatggt
     3481 tttaaaactc ttgctgaagg tcagcgcgta gagttcgaaa tcactaacgg tgccaaaggc
     3541 ccttctgctg caaacgtaat cgctctgtaa gatacgtcag caagaattca aaacccgctt
     3601 aatcagcggg ttttttttgg tctttagtgt gcggttgagg ccgaaaacag ccagaatgcc
     3661 agtgcggtca tggcaaaaga ccccagaagg ttgacgaaaa cgttcagtaa tgcccagcca
     3721 aagcggccct cttgtaacaa aaacaccact tctgccgaaa atgttgagaa ggttgttaga
     3781 ccgccacaaa atccggtggt gattaatact ttccacactg gatcaatgtt cgtcatcctg
     3841 ctgaaccagg cgaatcccat tcctatgatg aatgccccaa tcaggtttgc tgccagcgtc
     3901 cccaacggaa tcgcctgatg cagcgggtta aatcgcatac ttaacagcca tctcgctacg
     3961 cttcccgtac caccgccaat aaaaactgct aaaagaagtt gtaacactgc aaaatcctgc
     4021 tatttgattt gtatgagtga taagtgtaac gccgaataat cgtcgttggc gaattttacg
     4081 actctgacag gaggtggcaa tgctggttgc tgcaggacag tttgctgtta catctgtgtg
     4141 ggaaaagaac gctgagattt gtgcctcgtt gatggcgcag gcggcggaaa acgacgtatc
     4201 gctgtttgtc ctgccggaag cattgctggc gcgcgatgat catgatgcag atctatcggt
     4261 taaatcagca cagctgctgg aaggcgaatt cctcggacgt ttacggcgag aaagtaaacg
     4321 taacatgatg acgacaattc tgacgattca tcttccttca acgctggggc gcgcatggaa
     4381 tatgctggtg gcacttcagg caggaaacat cgtcgcccgt tatgccaaac tgcatctcta
     4441 tgatgcattt gccattcagg aatcacgcca tgttgatgct ggtaatgaaa tagctccgtt
     4501 actggaggtg gaagggatga aggtcggtct gatgacctgt tatgacttac gctttccaga
     4561 gctggcgctg gcacaggctt tacagggagc tgaaatcctg gtacttcctg ccgcctgggt
     4621 tcgtgggccg ctcaaagagc atcactggtc aacgttgctt gccgctcgtg cgctggatac
     4681 cacctgttat atggtggcgg cgggggagtg cgggaacaaa aatatcgggc aaagccggat
     4741 tatagatccc tttggcgtca ccattgcggc agcgtcagaa atgcctgcac tcattatggc
     4801 ggaagtgacg cccgaacgtg tgcgtcaggt gcgcgcgcaa ctgcccatct taaacaaccg
     4861 tcgctttgcg ccgccgcaat tattatgatg tttttttact cggcgcttga ttcaccttgt
     4921 tacagattgc tattgtgtgc gcgcgtcgaa tgaccgttaa tattctctgg tttttaaggc
     4981 gcgttctgtt gccggttata tgtcaagaag gtatctatgg gtgagattag tattaccaaa
     5041 ctgctggtag ttgcggcgct ggtcgttctg ctgtttggga ctaagaagtt acgtacgctg
     5101 ggcggagacc ttggagcggc cattaaaggg ttcaagaagg cgatgaatga tgacgatgct
     5161 gcggcgaaaa aaggcgcaga cgttgatctt caggctgaaa agctctctca taaagagtga
     5221 cgtggcgagc aggacgctcc ctcaatatct tgttcgatac aaaaaacccg cttcaaaaag
     5281 cgggtttttt atcagacaga tgtaagtaat tattacagga ttacttaact tccatccctt
     5341 tcgcctgcaa atcggcgtgg taagaagagc ggacaaacgg accgcatgca gcatgggtaa
     5401 agcccatcgc cagcgcttcg gctttcattt cgtcgaactc atccgggcta acgtaacgtt
     5461 gaaccggcag gtgatggcgg cttggctgca aatattgccc cagcgttaac atcgtcacac
     5521 catgacggcg caggtcgcgc attacctcaa taatttcttc attggtttca cccagaccca
     5581 ccatcagacc agacttggtc gggatttccg gatgcgcttc tttaaagcgt tccagcagct
     5641 tcagcgacca gttgtaatct gcgccaggcc gtacctgccg gtaaatacgc ggtacgtttt
     5701 ccaggttatg gttgaacaca tctgggggcg ttgcagtcag aatatccaga gcacgatcca
     5761 ttcgaccgcg gaaatccggc accagagttt caattttgat ttgcgggctt ttttcccgaa
     5821 tggcagtaat acaatccgca aagtgttggg caccgccatc gcgcaggtca tcacggtcaa
     5881 cggaggtgat aaccacataa cgcagcgcca tatcggcaat ggtctgcgcc agtttcactg
     5941 gttcattggc atcaggagct accgggcgac cgtgggcaac atcacagaac ggacaacggc
     6001 gggtacaaat agcgccgagg atcataaacg ttgctgtgcc gtggttgaag cattccgcca
     6061 ggttagggca ggaggcttcc tcgcagacag aatgcaggcc atttttgcgc attgcagctt
     6121 tgatgccctg gatacgtgta gagtccgctg gaagcttgat tttcatccat tccggcttgc
     6181 gcagcagggc ttcgcgctct gttgccacgt ttttaaccgg gataagggcc atcttatcgg
     6241 catcgcggta tttaacaccg cgttccatca caatgggttt actcatagcg tgcgtgttcc
     6301 agttgcgaat tacgaaggaa agcgtttcaa ttcaatagat tgttgtaatt atcaactatt
     6361 tttgaattaa cgactggcag tatatcattg aaacggacct gaaagcagcc aaagcggtcg
     6421 cgaaaatgta aaattgttgt tggattgtgc cattttatcg ttctgcactg tgatctggct
     6481 gtaacaatac tttttcaaaa tgcattacgg ataacatcta ttactccgtg caggatggga
     6541 tcacgcaggc tgaatttgtt gtagtggata gagaaatcta tttgctcctc atttaaggac
     6601 ggaaaaggca gtttttccag cggccagcaa cgactaaaca ggttataaaa acggctggga
     6661 ataatcgcta acatgtcact gttggcaacc agtgcagcga ttgtcaaaat gttgtagctg
     6721 gtgaaattga tttgccggtc cggaaacatc tcttgaactc tctgccgcag accgctgaaa
     6781 ttttgccctt ccggtaacag aagtacatgc gcagcgttgt cgatagtctc tctgtcatct
     6841 tctaaagaga gtagtggatt tcccttgcgg caaattaaca ccatattgtc ggtgaacaga
     6901 acatgatgtt gcaccgtacg attggtgcaa aacatgttat cgatgatgag atcggtttga
     6961 aactgactga gttggttttc cgcgtcgctg atgggcgggt tgcgcagtaa aagctgcgga
     7021 tagtgagttt taatcgcccg atagatgaca ggaaggacca gggctccgac cgagggagtg
     7081 gtcgcaatag ttatcgttcg ttgcttatca tagcttcctt cgatatccag cgcgccaaga
     7141 atggactcaa ggccctgact gatatactca tgtagatgca tcgcaaatgc ggtaggagtg
     7201 acactctggc ctttgcgaat aaacaatggg tcagggaata taacgcgcag tttctgaata
     7261 gactgactga ttgccgaggg ggtcagatta agcactttcg ctgcattaac gatcccttta
     7321 tgtacatata cagcttcaaa aatagtcaga aggttaagat caatatttcg taaggttgta
     7381 aatatttgtg gtttaccttc tgacgacttt ctgcttaaac agggttcaat ttgattatta
     7441 ctatccacgc acttactcca attttattca tggaaaaata atatttaaaa aattacaata
     7501 atcttatgtc taattggaac ggaacgcttt tgctcaccat aatcaactat ttcaataggt
     7561 taatcaatgg ggtgagttac ggagcaaggc gtggcaccgc atttctgcaa gtgataaaga
     7621 aaatatacaa acgcgaagga gatgtaaagc attagcaggt attatgagta atgacccagt
     7681 gtaaattggg ccattgatgt atggaattaa gcggtaatat attcgaagtc cggattgttt
     7741 agtagcgcta aaatattttc cagtaaacgt ggagcaatat tattagtcgt cgcttcgggt
     7801 ttccattgtg atattttagc catttccatt ccggcatacc cacaaggatt aatacgtaaa
     7861 aatggtgaaa gatccatatt gacgtttaat gccagaccgt ggaatgaaca accgcgtcga
     7921 attcgtaaac ccagtgagca aattttcttt tccccgacat agacaccagg cgcgtcagcc
     7981 cgaggatgcg cttctatacc cagttcagcc agggtattca ccactgtttg ctcaagcaag
     8041 gtcaccagtt cacgcacacc gagtttacgg cgtttcaggt taagcaacac atacatcacc
     8101 tgttgtcccg gcccgtgata agtcacctgc ccaccgcgat cgctctggat caccggaata
     8161 tcacccggca ttaaaatgtg ctccgctttt cctgcctgac cttgggtgaa taccggatag
     8221 tgctcgacca gccagatttc atcaagggta ctattatcgc gggtatcggt gaattcatgc
     8281 atagcccggg agattggctc gtaaggctga agaccgagct ggcggacaag aattttatcc
     8341 tgatacaaaa cggcatctcc gtggagaatg agtaaaagtg ggggaaaagt atatcacagc
     8401 gaggagagga gagttacccg accaggagcc gggtaacgga gaagcgagtt acagaaccat
     8461 gcggacaata tcgattttgc ccagttcttc atacagtgtt tcaacctgct cgatatgagt
     8521 ggcgttgata gtgatagata ccgagtggta gttgcctttg ctgcttggtt ttaccgttgg
     8581 ggtgtagtca cctggcgcat ggcgctgtac cacttcaacc acctgatcaa ccagctcagg
     8641 taacgcctgc cccataactt tgtaagtaaa aggagtaggg aattcaagca gttcgttaag
     8701 tttggttttc atgtcagctc cggcgtaacg taattaaata gcaactcccg ccagaaggtg
     8761 ggagtttttt tactgatgct tagtatatgg ggacggaaat tacactttca agtgttcaat
     8821 ttttaaccaa accagtgatg gaacattaat ttaatgtaat caatgatttt gccgaagaag
     8881 ttaccttccg ggatttcttg caacacaacc agcgggcgtt gctcgatcgt tttgccatca
     8941 agctggaagt tgatagttcc gacgacctga ttcttttgca gcggcgcatg caattcactg
     9001 ctgttcagca catagctggc tttcagatct ttcatgcgac cacgcggaat ggtcaggtac
     9061 acgtctttat caacccctaa cgaagcgcga tcagaatcac taaaccaaac cggttctgag
     9121 gcgaactctt tacctacttt cagtgggtta acggtttcga agaaacggaa gccccaggtt
     9181 agcagttttt tactttcggc ttcacggcct ttaaaagtac gtccacccat caccgcagaa
     9241 atcaagcgca tctggccttc agtcgcagaa gcaacaaggt tgtaacctgc tttgtcagtg
     9301 tgtccggttt tgatgccgtc gacattcagg ctgttatccc ataacaggcc gttacggttc
     9361 agctggcgaa taccgttaaa cgtaaattct ttttctttat agatcgagta ttcattcggt
     9421 acgtcacgga tcaatgcctg gccgatcagc gccatatctc gcgcggagct gtactgacca
     9481 tcagcatcca gaccatgtac cgtctggaag tgggtatttt tcaggcccag tgcgttaacg
     9541 tagctgttca tcaagccaac aaaagcgtcc tggctaccag cggcaaaatc ggccatggcg
     9601 acacaagcat cgttacccga ttgcaggtta ataccgcgga tcagctgaga aaccggaacc
     9661 tgcatgcccg gtttgaggaa catcagcgaa gaacctttaa acaccgggtt accggtggcc
     9721 catgcgtcgt tgccgatagt gactaaatca gtttctttaa atttaccggc tttcattgcc
     9781 tggccgataa cgtaactggt catcattttg gtcaggctgg caggatcgcg gcggacatct
     9841 gcgttctgtt cggcgagcac tttgccggag ttatagtcaa tcaggatgta ggactccgca
     9901 tcgatctgcg gtacacccgg gatcatagtt ttgatattca ggtcatcggc atgtgcagca
     9961 gagataaagg ctgtgcaaag agccgtggtg agcgccaggc gcttcatgat acgagcggaa
    10021 aaaatggtat tcatggtctg aactacgaca tccgtgatgg aattaaaaaa gtgccctact
    10081 atagcaaatg cactaccggc aggcatctga ctttccgcgt gactttgtta acgtcattta
    10141 cagaaattga cacatcagat gcctgcttta cgctactgcg cggtagtaat aaatgactgt
    10201 aattgggctt cggtttgcaa acgttgctgc aaggtactgg cttcggcttt gctggcgaat
    10261 gggccaagct ggatccgcca gaccgcgcca ttttgagtta cgcgaccggg gacgccgaac
    10321 ttctgtccca gttgctgttg gtactgttgc gcacgagcct gatcgcttac ggccccgact
    10381 tgcaccataa agttgccgct ggcgctttgc gaggcggctt gcggtgtcac cattgcaggc
    10441 gaggttgcag gcgttgtcga cgaagctgta acaacgggct gtggcgcagg cgtcggttcg
    10501 ctgccttcca gtacaccagg cgctaaggtc gtttgagcgc cgaggaaacc gctactggtt
    10561 accggcgcgc cggtcggatc ttcgcttttt agcgtcgaat tactgaccgg aagaatgtca
    10621 ccctgcgggc cagacactga acttgttccc gcgccaccgc ttaaatcggg aggtgcaggc
    10681 agggcataag tctgtttggc gactgtggta caagccatac caggaccaga aagcgaacca
    10741 tcctgggcga caataatcgg atcgatacga actttggtgt tgtttgacgt gttaagacgg
    10801 tcagctgccg cgcgagaaag cgaaataacg cggtcgttgc cgtaagggcc gcgatcatta
    10861 atgcgcacca cgatcattcg cccgttagcc aggttagtga ttctggcgta gctggggatc
    10921 ggaagcgttg gatgggccgc cgtcagcttt gtcggatcga aagcttcgcc agaggccgtc
    10981 aggttactgc ctggttcggc atcatagatt gccgccagtc ccgcctggct aaatcgagac
    11041 ggatcctgca cgattttgta gcttttaccg tcgcgctggt aatcctgatt tgccgtcgcg
    11101 ttcagtggtt cgaaacgcgg gtccgccccg ctaatttcaa ctatggggcc gttacatacc
    11161 gcaggctgcg gtacacttac cgtctgttgc tgaccatcat cgcttgtaca tgccgcgagc
    11221 attcctgccg cgatgcagat cccgagccac tgcttacgca ttgcgcacct cttacacgct
    11281 tttcgacaac attttcctgt gggtgtggat tgacattaca atcccgaacc cagccatcag
    11341 cacaattagc gccgatcctc cataactgac cagtgggagc ggaaccccta caaccggcag
    11401 aataccgctt accataccaa tatttacgaa gacataaacg aataatatca gcattaagcc
    11461 accagccatg acgcgaccaa aggtggtttg cgctctggcg gctatccaca gcccgcgcat
    11521 gatcagcaga atgtagagag cgagcagaat cagaatgccc actaatccca gctcttccgc
    11581 cagtaccgcg aagataaagt cagtatggcg ttcggggaga aattcaagct gtgactgagt
    11641 gccgtgcagc cagcctttgc cgcgtaatcc gccggagcca atagcaattt tagactgaat
    11701 aatgtgatag cccgcgccga gtgggtctga ttccgggtcc aggagcatca ttacgcgctg
    11761 gcgctggtaa tcatgcatca ggaagaacca cagaatcgga atgaacgccg ctaccagcac
    11821 tactgcgacg ccaatcagac gccagctaag gccagagagg aacagtacaa acagaccgga
    11881 aagcgcaacg aggattgatg ttcccaggtc aggctgtgca gccaccagca gcgtgggcat
    11941 aaatatcagc accagcgcga tgccagtgtt cttcaacgat ggcgggcaaa cgtcgcggtt
    12001 gataaagcgc gcaaccatca gtggtacggc tattttggca atttccgacg gctgaaaacg
    12061 aacaataccg aggtccagcc agcgttgagc gcctttagag atggctccga aagcatctac
    12121 cgccaccagc aaaataatac agatgatata gagatagggg gcccagcctt cataaacgcg
    12181 tggaggaatt tgcgccatca ccaccatgat gaccagaccc atcgcgattt ggccgatttt
    12241 acgctccatc atgccaatat cctgaccgct ggcgctccag ataaccaggg cgctgtaaac
    12301 cagcaatgcc agtaaaatca gcagcattgt gggatcgaga tggactttat cccagaatgt
    12361 ttttttattc ggattatccg tcatgattaa tggtcctccg ctgcggcaac cgctggattt
    12421 tccgcaggca gatcggtgtt gttatcaccc agcataatgt ggtcgaggat ctggcgcatc
    12481 agcgtaccaa ccgccggacc cgcgccgccg ttctccagaa tcatggcgac agccacttgc
    12541 ggattgttgt atggcgcaaa ggcggtcatc agtttgtggt cacgtaaacg ctcggcaatt
    12601 ttgtgcgcat tataggtttc gttcgctttc agaccgaaga cctgagcggt accagatttc
    12661 gccgcaattt tgtacggtgc gctggcaaag tatttatgcg ccgtaccatt agggcggtta
    12721 gcaacaccgt acataccgtc tttcgccagc tcccagtaac cggaatgaat atcgccgacg
    12781 ggcggttcat gcggttgtac ccatggcacc tgtttgccgt cttcggcggt gctcatcagc
    12841 aaatgcggaa ccttcacgat accgtcatta atcaggatca tcagtgcctt actcatctgg
    12901 attggggtcg ctgtccagta accctggccg ataccaaccg gaatggtgtc accctgatac
    12961 cacggttttt taaagcgttt ctgtttccat tcgcgggtag gcatgttgcc ggaacgttct
    13021 tccgccaggt cgataccggt gtaatgacca taaccgaatt tacccatcca ttcggagagg
    13081 cgatcgatcc ccatatcgta ggccacctga tagaagaagg tatccgcaga ttcttccagc
    13141 gatcttgtga cattcagacg cccgtggccc cattttttcc agtcacgata acgtttttcc
    13201 gaccctggta gttgccacca gcctgggtca aacagcgtcg tattgcgcgt gatcaccccg
    13261 gcgcttaatg ccgaaaccgc cacatagggt ttaactgtag acgcgggagg ataaaccccc
    13321 tgtgtggcgc ggttcaccag cggtgtattc gggtcgttca gcaaggcgga ataatctttg
    13381 ctggagatac cgtcaacaaa caggtttggg tcataactag gcgtggaaac cagcgccagc
    13441 accccacctg tacgcggatc ggtaacaacc acagctgcgc ggctacccgc cagcagcgtt
    13501 tcaatatatt gctggagttt gagatccagc gtcaggtaaa tatcgtgtcc ggcttgcggt
    13561 ggtacttctt ttaactggcg gataacacgc ccacggttgt taacttcaac ctcttcataa
    13621 ccggtctgac cgtgcagcac gtcttcatag taacgctcaa tgcccagctt accaatatca
    13681 tgcgttgccg catagttggc cagtttgccg tcattattca ggcgttcgac gtctttatcg
    13741 ttgattttcg acacatatcc gatgacgtgg gtcaacgccg aaccgtaagg atagtaacga
    13801 cgtttatagc ctttaacttc gacacccgga aaacggtact gattgacggc aaagcgagct
    13861 acttgtactt cggtcaggtt agttttcacc ggaatagagg tgaaacggtg tgaacgtgcg
    13921 cgctcttttc ggaatgcagc aatatcgtca tcggtcagat ctaccacgct gcgcaaagcg
    13981 tccagcgttt gctgcacgtt atcgactttc tccggcatca tttctatctg gtagatagtg
    14041 cggttgaggg ccagagggat accgttacgg tcgtagataa tgccgcggct gggcgcgata
    14101 ggcaccagct taatgcggtt ttcattagag cgggtctggt agtcggtaaa gcgaacaatt
    14161 tgcagattat acaggttggc gataagcacg ccggtcagca gcaaaatccc caaaaaggcg
    14221 accagcgccc ggcgcacaaa cagcgcggac tctgccgtat agtcgcgaaa agagttctgt
    14281 agtttcatcc gctgcgtttt ctactcaaag ctcccttatc actcacggtg ataaggatgg
    14341 ttggtggtga tgctccacgc ccggtacaga ctctctgcga ccagcacgcg aaccagcgga
    14401 tgggggaggg taagcgccga cagcgaccag ctctgctcag ccgccgcttt acaggcaggc
    14461 gacaaccctt caggcccgcc aatcagtaga cttacgtcgc gaccatccag cttccagcgt
    14521 tccagctcag cggctaactg cggcgtatcc cagggcttgc ctggaatatc gaggatgaca
    14581 atgcggtttt tgcctgcggc cgccaacatc tgctcaccct ctttgtcgag tatgcgcttg
    14641 atgtccgcat tcttgccgcg tttgccggcc ggaatttcaa tcagctcgaa gggcatatct
    14701 ttcggaaaac gacgcaggta ctcggtaaaa ccggtttgta cccagtccgg cattttcgtt
    14761 cccacggcga caagttgcag cttcacgcat taactccaga gtttttccag ttcatacagg
    14821 cgacggctct cttcctgcat gacatggaca atcacatcgc ccaaatccac gacaatccag
    14881 tcggcgctgt tttcaccttc tacgccgagc ggtaacaggc ccgctgcgcg agactcctgc
    14941 acaacgtggt cagcaatgga cataacatga cggctggacg tacccgtaca gatgatcatg
    15001 cagtcggtga tgctggattt gccttgaacg tctaaggcga tgatgtcctg acctttgagg
    15061 tcatcaattt tgtcgataac aaaatcctgg agtgctttac cctgcaagtt ttccccctgg
    15121 gtgaatcaaa tagataaaaa tggtctgtca gtatacctga accagaggcg atttcgggac
    15181 aattgtcgcc gaatcggctt tcgaaagtgg gctatcatcc caccccgcgc cgcagattgc
    15241 atcgccattt ttgtaaaaca atttctacaa agtcgtgtct ggcggaaaaa gtctggctgc
    15301 ggagaatatc agcctgcccg gggctgtcaa tggtcgttgc ggcttacccg taaaaaaaca
    15361 gaaaagtcat gcattctcga ccccgatggc acggctattg aggacgcgta gcgtcgcgaa
    15421 tttttggttg atatcaatgg cgctccaaca cccctggtca acgcgaaaat gccacataga
    15481 ctcggcaggc atgccaatta aacgagcaat taacagactc agtacgccct gatggctgac
    15541 gattaaaata ttctgatagt gctgatattc actaagcctt gcgataaagc gttccacacg
    15601 ttgcgaaaat gcctgaaatc cttcaccgtt cgtggggata gcatgctgcc agtcattgca
    15661 ccacgcgcta tagttttcgg catcttcttg catgaggtcg cgatgatgtc gcatctccca
    15721 gtcgccaaaa aacatttcgt tgagttcagg gatgatatga acggggagct ggcggtcact
    15781 gagaaccagt cgcgcggtat gctgtgcccg ttccagttca ctgcataaaa ccaggtcaaa
    15841 ggaaacatca tctagcagtg tatgcagatt ttgcgcttgc tcaataccgc gtgcggtcag
    15901 gggtgtgggc gcatgaccgc tgtacagacc atcgacattc gcttgtgttt cgccatgacg
    15961 aattaaccac agtcgcatca tgccctccgt aacgacaggt atcagcgata caagccttgt
    16021 tggttaatgt aagtcaatac cggttccggc aataaatcct cacacgattc accgttttgc
    16081 aaacgttcgc ggatgatggt cgccgagatg ttaaaccacg gcgtttcagc cagataaatt
    16141 ttaccggcag gctgaaggtg aagatcttcc gggttatgtg tcaaatgatc ttccagccat
    16201 tgctggtatt gcggttgcgc catttcaagt gggtaacctg gacgccgaca gacgatcaaa
    16261 tgtgcattgt cgagtatcgt ttcgtattcg taccaggtcg gaaaggtcag caatgaatcc
    16321 tgaccaataa taaacgccag cggcacgtcc ggtccttgtt cctgccgcca ctctttcagt
    16381 gtttgtgcag tgtaagaggg ggcattgcgc tttagctcgc gttcatcaag agtaaataat
    16441 ggcttgtcgg caatcgccag ttcaagcatg tgtttacgct gcacgctgtt cgcttccggc
    16501 tggggacgat gcggaggaac attattaggg atgattgtga cccgcgtcag accaatcaaa
    16561 ttcgccagcg tttccacggg ttttagatga ccatagtgca ccggatcaaa ggtgccgcca
    16621 aacagagcct gtaaagattt catatcaacc gtcgataaat acgtccgcca ggggtttatg
    16681 gcacaacaga agagataacc cttccagctc tgcccacact gactgaccgt aatcttgttt
    16741 gagggtgagt tccgttcgtg tcaggagttg cacggcctga cgtaactgcg gctgacttaa
    16801 gcgatttaac gcctcgccca tcatgccccg gcggttctgc catacccgat gcttatcaaa
    16861 caacgcacgc agtggcgtat gggcagactg gcgtttcagg ttaaccagta acaacagttc
    16921 acgttgtaat gtgcgcaaca aaataaccgg ctcgctgcct tccagacgca gttgctgaag
    16981 aatatgcaac gcgcgcttac tttttcccat caacaaagca tcaacccaat gaaaaggggt
    17041 gaaatgcgcg gcatcattca ccgcctgttc aacgcgcggt aatgtcagtt tgccgtctgg
    17101 ccagagcagc gataaacgct ccagtgcctg agccagcgcc agcaggttac cttcataaca
    17161 gtagcagagc acctgattcg ccgcgtcatc cagttctaag ttgagctgtt ttgcgcgcac
    17221 agcaacccag cggggaagct gagcctgctc cggtgtctga caggtcacct gcacgctgcg
    17281 attcgcaaga gcagtaaacc aggcggcatt ttcttgcgct ttgcttaatt tattaccgcg
    17341 gacgatcaac agcaggtcgt catgcagaag tccggtgagt gtgagaagtt gttcattgat
    17401 cgccgcattc ggtccgtttt ctggtaacaa cagcaatagc gtttgtcgac tggcaaacag
    17461 actcatagcc tggcataacg aaaagatcgc attccagtca gtgttgggat caatggaaaa
    17521 agtgtggtgt tcttcgaatc cttgtgccgc agctacctgg cgaacagcgt cctggctttc
    17581 ctgcaataac agaggatcgt taccaagtaa aagatacgcc gcgcgcagcc cttcattgag
    17641 ctgcgcgcgg agttgttccg ggtacaaccg aatcatcagt tacccagcat ggtggagacg
    17701 cgcgcaggcg ttgccggagt atccgttgtg gtcgacgtct gttcttcgtc ggaacgaata
    17761 tccgcagcac ggatgcttgg cagcttacga atcagctgtt cggcagcacg gtcgtacatc
    17821 tcttttacga tcatgtcttg ttcgttatct ttcgctaacg ccatttgcgg gttatcgaag
    17881 aatgaacgga agactttggc gctaattggg tagatatcac ggccggggat caacacggtc
    17941 gcattcaccg tcatgatcat ctgatactct gctgtttgac cgttacggaa taccgatgcg
    18001 gtatctttcg cgatgctcac tttacccaaa cgcagggatg gaacgtcctt acgcgtggtt
    18061 tctttatcaa gcaactcgac accattcaga cgtaactggt tacgcaccgc acggcttaat
    18121 ggcccgttcg gatcgcctga gtccaggatc atgaccttca tagtggaagg aacctgcgtg
    18181 gtgtcacgca gatgccagcc acacccggcg gtgattaaca ccgccagaga taacaacaat
    18241 gttgccagat atcgcacgct tcctcccgcg cttagccaac gaccagattg aggagtttac
    18301 ctggtacgta aatcacttta cgtacagtaa cgccatcaag atattttgct accagatgtt
    18361 cctggccagc acgttcgcga acctgttctt ccgttgcgtc caccggaacg gtgattttgg
    18421 cacggacttt accgttaacc tgcaccacga ccagcgtgga gtcttccacc atcgcttttt
    18481 cgtcagcaac cggccacggc gcgttgtcga tatcgccttc gcctttgagt tcctgccaca
    18541 gcgtgaagca gatgtgcggg gtgaacgggt tgagcatacg gacaacggcc agcagtgctt
    18601 cctgcatcag agcgcgatcc tgctcgccat cggtcggtgc tttcgccagt ttgttcatca
    18661 gctccataat cgccgcaatt gcggtgttga aggtctgacg acggccgata tcatcggtca
    18721 ctttagcgat agttttatgc acatcgcgac gcagcgcttt ctgatcttca gtcagcgcat
    18781 caacgttcag tgccgcaaca tcaccttttg ctgtgtgctc gtacaccagt ttccagacac
    18841 gtttcaggaa gcggttagcc ccttccacac cggattcctg ccattcgaga gtcatatcag
    18901 ccggagaagc aaacatcata aacagacgaa cggtgtccgc gccgtaacgt tcaaccatca
    18961 cctgcgggtc gataccgttg ttcttcgact tggacatttt gctcatgccg gtataaacca
    19021 gttcatggcc tgccgcatct ttcgctttca cgatacggcc tttctcgtca cgttcaacga
    19081 tagcatcaac cggggaaacc cagttacgtt cgccgttttc gccaacatag tagaaggcat
    19141 ctgccagcac cataccctga cacagcaact gtttcgctgg ttcgtcagag ttcaccatgc
    19201 ctgcatcacg catcagtttg tggaagaagc ggaagtagag caggtgcata atggcgtgtt
    19261 cgataccacc aatgtagata tccaccggca gccagtagtt agccgcttcg gaatccagca
    19321 taccttcttt gtactctggg caagtgtagc gcgcatagta ccaggaggac tccataaagg
    19381 tgtcgaaagt gtcggtttca cgcagcgctg gcataccgtt aacggtagtt tttgcccact
    19441 ccggatctgc tttaatcggg ctggtaatgc cgtccatcac cacatcttcc ggcaggatca
    19501 ccggcagctg gtcgtccggg gttggcatta cggtaccgtc ttccagcgtc accatcggaa
    19561 tcggcgcgcc ccagtaacgc tgacgagaaa caccccagtc gcgcaggcgg tagttcactt
    19621 tacgctcgcc aacgcccatc gcagtcagtt tatcggcgat ggcgttgaag gccgcttcat
    19681 ggtcaagacc gttgaactcg ccagagttga acagcacgcc tttttcagtc agggcttgct
    19741 gagaaagatc tggctcagag ccgtcagccg ccaggataac cggtttgatg ttcaggccgt
    19801 atttagaggc aaactcgtag tcgcgctggt cgtgacccgg aaccgccata actgcgcccg
    19861 tgccgtactc catcaatacg aagtttgctg cccaaacggg aatttcttcg cccgttaatg
    19921 ggtgaacagc tttaaagcca gtatcgacgc cttttttctc cagcgtcgcc atttcagctt
    19981 cggcaacttt ggtgttacgg cattcgtcaa taaaggccgc cagttcagga ttattttccg
    20041 ccgctttctg cgccagcgga tgacccgcag ctaccgccag gtaggtacaa cccataaagg
    20101 tgtccgggcg ggtagtgtaa acggtcagcg tgttgtcata gtcgttaacg ttgaaggtga
    20161 tctccacgcc ttcggaacga ccgatccagt tacgctgcat ggttttaacg gtgtctggcc
    20221 agtgatccag tttatccaga tcgttgagca gctcgtcagc gtaagcagtg attttgataa
    20281 accactgcgg gatctcttta cgttcaactt tggtatcgca gcgccagcag cagccgtcga
    20341 taacttgttc gttcgccagt acggtctggt cgttcgggca ccagttgacc gcagaagtct
    20401 ttttatatac caggcctttt ttatacagct cggtgaagaa tttctgttcc caacggtagt
    20461 attccggcgt acaggttgcc agctcgcggc tccagtcata accaaagccc agcattttga
    20521 gctggttttt catatacgcg atgttgtcgt acgtccacgg tgccggagcg gtgttgtttt
    20581 tcaccgccgc gccttccgca ggcagaccaa acgcgtccca gccgatcggc tgcagaacgt
    20641 ttttgcccag catgcgctgg taacgggcga tcacgtcacc gatggtgtag ttacgtacgt
    20701 ggcccatgtg tagtcgacca gaaggatagg gaagcataga caggcagtaa tacttctctt
    20761 tgctctcgtc ttcggttact tcaaatgtgc gcttctcatc ccaatgaagc tgtactttgg
    20821 attctatctc ttccgggcgg tattgctctt gcatggcagc cagtggtcct gttttcaata
    20881 cggctacaaa tgtagcgttg aggtggtttt tcagatccgc atagcatagc ccaaacgtcc
    20941 gcgtcaaaac agcctttcgc gcactcgacg ttgaaatgat gcccggatta ttcgtacatt
    21001 aatttacaga gtttgtgggc gtattagcaa agcaaggaac aaagaacgtc tattattata
    21061 gtcagttaac gacccgggag atgaaacgat gaacaaggtt gctcaatatt accgtgaact
    21121 ggtcgcgtca ctgaacgaac gcctgcgcaa tggcgaacgt gatatcgacg cactggtgga
    21181 acaggcgcgc gagcgcgtaa taaaaacagg ggagttaacg cgaaccgagc tcgatgagct
    21241 gacgcgagct gtcagacgtg acctggaaga gttcgccatg agctatgaag agagcctgaa
    21301 agaagaatct gacagcgtct ttatgcgggt gattaaagaa agcttgtggc aggagctggc
    21361 agacatcacc gataaaacgc agcttgaatg gcgcgaagtt ttccaggacc tcaatcatca
    21421 tggggtttat cacagcggag aagtggtcgg gctgggaaat ctggtctgcg agaaatgtca
    21481 cttccatctc ccgatctaca caccggaagt gctgacgcta tgcccgaaat gtggttatga
    21541 ccagttccag agacgcccgt ttgagccgta atagtctgat ttcgcatagc aataaagaga
    21601 atatgggcag aatgaaattc cccggccctt gtttttattt ctacagtaaa gcttcagtct
    21661 cacgaatttc ctgactgaca tctctggttc cggatgactg aactttacga tacaggtcga
    21721 gagcctgtcg gagatcgaca ggtcttcctt ctccgtagtg atacataatg gccaggttat
    21781 accaggcagt ggcgtcattg cattcagcgg ctttcgtaaa ccattcaaag gcagcctgat
    21841 aatctttctc aacgccttgt ccgtgtttat acatataacc gatgttgacg taagcgccga
    21901 cgctttcctg agcggcagcc ttcagatacc agtacatcgc ttgcttatag tcctggtcca
    21961 ccccttcgcc agcgttataa tcccatgcaa tttgaaactg ggcgtgccga ttaccctgct
    22021 gtgcgctttt caaataccag aatgcggcga gtgtcttgtt ttgagcaacg ccttctccgt
    22081 ctttataaag atcggccaga ttgtcttgtg cgtaactatg gccttgtaat gcagcttgtt
    22141 tgtaccagaa aaatgccagc gtatagtctt gtgcgactcc gttgccattt ctgtacatcc
    22201 atccaagatt attttgcgca taagacattc cgctctccgc ggctatctga taccaggtaa
    22261 ccgcttcttt atagttgggg tcttctccac gatccagtat ccagccaagg gcgttggaag
    22321 cgaaagtatg gccttgtagg gcagcttttt tcagccagaa gatggcttgc tcattatctt
    22381 tatgacggct tttatcctcg gtatactttt gccctaagga atactgtgct tcacaatgac
    22441 cttgctcagc ggccagcttc agccagtaaa aggccttttc gtcgtccgga gagtcaattg
    22501 cgctatcgcg attataataa aacccaacaa tatattgagc ctcgcaatcg cctttttcag
    22561 cacgttcgat aatctcatct attgataaat tatcgcagca acttgacgta aaaattatta
    22621 tcatttcctt atggttgtag ataaaatata ttatattttt atgtgttcca tttttgcgta
    22681 agccttattt tatgcgtatt atgttgcgtc agaaaagtat aaataactga gtctcgaaat
    22741 gtagtttgca ttcattaaat gaatgtttaa ggataaatta tatggacatg gggtcgcaaa
    22801 aaatattgtt tgcactttcc actccgatgg aaatacgcaa tgaatgctgt ttaccttcgc
    22861 attcatcccc caaaatgtat ttaggtacat gcttttttga cctttcttcc tcatggggaa
    22921 ttgatgaccg tgatgacctg ctgcgcacaa ttcaccgaat gatcgagaac ggtcatgccg
    22981 ctcggttggc agggttttat caccgctggt ttcgttattc tccatgtgaa tggcgtgact
    23041 atcttgctga attaaatgag caaggtcagg cttatgcgca atttgtcgcc agcacagccg
    23101 aatgctgtgg tgagggagga attaaagcat gggattatgt ccggatgggt tttttaagtc
    23161 gaatgggcgt acttaataac tggttgagtg aagaagaaag tctatggatc ctgtcgcgta
    23221 ttcatctacg ggctctacgt tattatagta actggcggca atactttgcc ggttatacct
    23281 ttggtcggca atattggcag tctcccgaag atgatcatct gcctttatta cgcgaattct
    23341 tagcgcgtaa agaatacgac gattccggca atgatatgtt ttatcaatta tttgccagtg
    23401 atgatgcgta ttaccctacc ttgtcctggc aaccactggc tgactacccc acatgcccgg
    23461 aaacgcttaa ggatatgagc gacttatgaa aaattgctgg aagatcctcg atatagagga
    23521 aacgactgac gtcgatatta tccgccgcgc ttatctggcg ctgttaccgt cctttcatcc
    23581 agaaaccgat ccgcagggtt ttaaacaact tcgtcaggcg tatgaggagg cgctacggat
    23641 tgcgcagtcg cctgctaaat ctgtttggca accagaagaa tatgaggtag cagaacatga
    23701 aattctgctc gcctttcgcg cgttacttgc ctctgatagc gaacgttttc tgccctccgc
    23761 ctggcagcga ttcattcagc aattaaatta ttgctcgatg gaagagattg atgaattacg
    23821 ctggtcgctg tgcacaatcg ccatgaacac tgcccattta tccttcgagt gcgtggtgtt
    23881 attagcagaa agattgcggt ggttgcagga ggaaaacgtc ggggaaatag acgaagaaga
    23941 actggaatcc tttttatatg ccattgcgaa ggggaatgtt tttaacttcc agaccattct
    24001 gcatctgccc gttgccgtgc aaaatgacac catcgatttt taccaaatgt tcgctcggat
    24061 ttggtcatcg catccagaat ggctgacatt gtatttagcg caacatcgcg cagtgattat
    24121 ccccgatgat gcaaaactgc acagaaattt actccgctgg tatagcgcaa gccgcctggg
    24181 aatccccgaa ctcctggatt acgcccggtc gtggcgggaa gctgaacctg ataatgaaga
    24241 tgcgcgttat tatgaatacg cacaacgcgt ctattgtgga gaaggcgaaa gcctgctggc
    24301 agaactttgt tactactggc gcgagtatcc ctccacccag gcggatgctt taatgttgca
    24361 atggtgccgt cagcatcggg tcgattatta cccattagtg gtgatgatga ttgaagcgcg
    24421 tgatctggtt aacgatcagg gaaaaccgct actttatgtc cccggcgaca gcgcccgtac
    24481 gcgttttcat ttatacgaaa tactcagcga tgaaaaactc tctgcgctgg ggcgttcact
    24541 ggtcgagatg gttttgcaca aaggacgtaa gccgcggatc tcactcacgc gtgatacaga
    24601 acatccctta tggccattat atttagttgc caaacaatta gtgcaggcca gccaaccgac
    24661 agaagaatca ttaatgccga ttgtgagccg ccttgatgca gaagatcgtt gtccactgga
    24721 agcattaatt attcgtcgat tattaattca ggcggcgaat tttaccggtc aggaaaccgt
    24781 cgagccggag ccgcaaccgc agccaatgcc cgttgacgat ggcgggctgg gctgtctggg
    24841 cgtcatcaaa attattttct acatttttat ctttgccggt ttgataggga aaatactcca
    24901 tctcttcggg tgacagtcga cgttaatgtg cgggaagtcc ggcaagtaat ctttccgcat
    24961 ttttgtaatg ttgtttggcg gccagtttta accatttttg agattcataa tggttaacat
    25021 taataccagt gccatttctg tagatcaaac ccaaagtata ttgagcatga gcataccctt
    25081 gttcagctgc ttccttatac cagagaattg ctttacgggt atcgggtttg actcccaggc
    25141 ctttcaggta gatccacccc agtttgcttt gtgcgcgcgg attttcctgt tctgctgatt
    25201 gctcatacca gtatctcgcc tgtgtataat ccagtggttt accgtcgcta ccatgaagat
    25261 aaaattcgcc aaggcgatat tgcgatgttg agtgaccatg aatcgcggaa agctccgtgg
    25321 ctgccgtggt taacggtaga ctgcctggtg atcttaaata atcaaaggca taattcacca
    25381 tggctttatc acgcaacgtc gttttcttgg gttggctaat acaggtaatt gctgcaacta
    25441 taaa
//
LOCUS       DADLXX010000046        25397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLXX010000046 DADLXX010000000
VERSION     DADLXX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.046"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..116
                     /locus_tag="JNX27_001906"
     CDS             <1..116
                     /locus_tag="JNX27_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1315 domain-containing protein"
                     /protein_id="HAY7883140.1"
                     /translation="TEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            486..1562
                     /locus_tag="JNX27_001907"
     CDS             486..1562
                     /locus_tag="JNX27_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7883141.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            1589..2957
                     /locus_tag="JNX27_001908"
                     /pseudo
     CDS             1589..2957
                     /locus_tag="JNX27_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2974..4019
                     /locus_tag="JNX27_001909"
                     /pseudo
     CDS             2974..4019
                     /locus_tag="JNX27_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            4040..4876
                     /locus_tag="JNX27_001910"
     CDS             4040..4876
                     /locus_tag="JNX27_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY7883142.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            4881..5829
                     /locus_tag="JNX27_001911"
                     /pseudo
     CDS             4881..5829
                     /locus_tag="JNX27_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            5839..6819
                     /gene="ydjG"
                     /locus_tag="JNX27_001912"
     CDS             5839..6819
                     /gene="ydjG"
                     /locus_tag="JNX27_001912"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY7883143.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDPALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            6956..7714
                     /locus_tag="JNX27_001913"
     CDS             6956..7714
                     /locus_tag="JNX27_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7883144.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            7832..9190
                     /locus_tag="JNX27_001914"
     CDS             7832..9190
                     /locus_tag="JNX27_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883145.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9283..9924)
                     /gene="pncA"
                     /locus_tag="JNX27_001915"
     CDS             complement(9283..9924)
                     /gene="pncA"
                     /locus_tag="JNX27_001915"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7883146.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(9935..10951)
                     /gene="ansA"
                     /locus_tag="JNX27_001916"
     CDS             complement(9935..10951)
                     /gene="ansA"
                     /locus_tag="JNX27_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7883147.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(11118..12974)
                     /gene="sppA"
                     /locus_tag="JNX27_001917"
     CDS             complement(11118..12974)
                     /gene="sppA"
                     /locus_tag="JNX27_001917"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7883148.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            13135..13686
                     /locus_tag="JNX27_001918"
     CDS             13135..13686
                     /locus_tag="JNX27_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7883149.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13803..14846
                     /gene="selD"
                     /locus_tag="JNX27_001919"
     CDS             13803..14846
                     /gene="selD"
                     /locus_tag="JNX27_001919"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7883150.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSKMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14851..16812
                     /locus_tag="JNX27_001920"
     CDS             14851..16812
                     /locus_tag="JNX27_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7883151.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIEQDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            16939..17979
                     /locus_tag="JNX27_001921"
     CDS             16939..17979
                     /locus_tag="JNX27_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7883152.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMRLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(18096..19439)
                     /gene="gdhA"
                     /locus_tag="JNX27_001922"
     CDS             complement(18096..19439)
                     /gene="gdhA"
                     /locus_tag="JNX27_001922"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7883153.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARAITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            19675..19947
                     /locus_tag="JNX27_001923"
     CDS             19675..19947
                     /locus_tag="JNX27_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7883154.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(19913..20320)
                     /gene="nudG"
                     /locus_tag="JNX27_001924"
     CDS             complement(19913..20320)
                     /gene="nudG"
                     /locus_tag="JNX27_001924"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7883155.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            20407..21027
                     /locus_tag="JNX27_001925"
     CDS             20407..21027
                     /locus_tag="JNX27_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7883156.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(21036..22343)
                     /locus_tag="JNX27_001926"
                     /pseudo
     CDS             complement(21036..22343)
                     /locus_tag="JNX27_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            complement(22410..23063)
                     /locus_tag="JNX27_001927"
     CDS             complement(22410..23063)
                     /locus_tag="JNX27_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7883157.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(23063..24597)
                     /locus_tag="JNX27_001928"
                     /pseudo
     CDS             complement(23063..24597)
                     /locus_tag="JNX27_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(24570..25397)
                     /locus_tag="JNX27_001929"
                     /pseudo
     CDS             complement(24570..25397)
                     /locus_tag="JNX27_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         6333 a         6083 c         6635 g         6346 t
ORIGIN      
        1 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
       61 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
      121 ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc acgactcgtc
      181 tcaattatct cgacccgacc tggctaagtc ccagtccgat ctcttgcagt cgcgttcacg
      241 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcgaa
      301 tttgctcttt ttgcgtcccg ccttccacca acctgtaaca gacccagcca ttttctgcgt
      361 aattgtgatc taagttgtaa aatgcaggtc attatctgtg ttgtgcagat ttaatgttga
      421 cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac acagaggatg
      481 acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag atgattcatg
      541 tcccacaacc catttgtggc ccggaagacg tagtgattga aattaaagcc gcggcaatct
      601 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
      661 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
      721 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgt ccggcctgtg
      781 aacagggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
      841 ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa attcatcgtc
      901 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
      961 tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttcctggt caggatgttg
     1021 tcgtcattgg cactggccca ctcgggctgt tttccgtaca aatggcgcgg attatggggg
     1081 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
     1141 aagaactggg ggcgacggca gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc
     1201 agcaaatttg tggcaaagat aatctgggac tggtgattga atgctccggt gccaatatcg
     1261 cactgaaaca agccatcgaa atgctccgcc cgaatgggga agtggtacgc gttggaatgg
     1321 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaataaa agcatcattg
     1381 ggcatatggc ctatgactcc acctcatggc gtaacgctat ccggctatta gccagcggcg
     1441 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
     1501 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
     1561 aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc attgtggtgt
     1621 acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt
     1681 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
     1741 gttgggctgg acagataatt ccaccacagc cacattctca gcaatcacga ccgccggaat
     1801 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
     1861 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caccgaatat
     1921 ggattttctt attgcctgcc gttttgtgat ggacgttggg ctgggagctt tactggttac
     1981 actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg
     2041 ggtttccttt attggcaact ggtcatatcc gctctgctca ctgattgcga tgggactcac
     2101 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttattcctg caatattgtc
     2161 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
     2221 gtgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
     2281 ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag cgccacaagc
     2341 ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat taggctcttg
     2401 tgtgctgatt accatgaacg ttgtgcagta cacactaatt aactggttgc caacaatatt
     2461 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
     2521 cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
     2581 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctaccagt atgttgctca
     2641 taacgctgat tggtttcttc ctgattactt tcgtctatat gtacgtttgc tatgcctcgg
     2701 cagtgtatgt ccctgaaatc tggccgacag aggccaaact ccgtggctcc ggtctggcga
     2761 atgcggtagg gcgaatcagt ggtattgccg caccttatgc cgttgcagtg ctgctcagta
     2821 gttatggtgt aacgggagtc tttattcttc tgggggctgt ttcaattatt gtcgcaattg
     2881 ctatcgccac cattggaatt gaaaccaaag gtgtctccgt tgaaagttta agtattgatg
     2941 cagtagccaa taaataatac gggtggattc aaaatgaaaa attcaaaagc aatattgcag
     3001 gtgccgggca caatgaaaat tatttcagca gaaataccag tgcctaaaga agatgaagtt
     3061 ttgattaaag tagaatatgt cggtatttgt ggttcagatg tacatggttt tgaatcaggc
     3121 ccgtttattc cgcctaaaga cccaaatcaa gaaattggcc tgggtcatga atgcgccggg
     3181 acggttgtgg ctgtgggaag ccgcgtgcgc aaatttaaac cgggggatcg ggtaaatatc
     3241 gaacctggcg ttccttgcgg tcactgtcgt tactgtctgg aaggcaaata taacatctgc
     3301 ccggacgttg attttatggc gacacaaccc aactaccgcg gcgcattaac gcactatctg
     3361 tgtcatccgg agagctttac ttacaaactg cccgacaata tggacacgat ggaaggggcg
     3421 ctggtggagc ctgccgcagt cgggatgcat gccgcgatgc tggcagatgt taaaccgggt
     3481 aagaagataa ttattctggg agcaggttgt attggtttga tgacgttgca agcgtgcaaa
     3541 tgcctgggcg caacggaaat tgccgtcgtt gatgtactgg aaaaacgtct ggcaatgggg
     3601 gaacagcttg gtgcgacagt ggttattaat ggcgcaaaag aggacactat tgcacgctgt
     3661 cagcaattta ccgaagacat gggcgcagat attgttttcg aaacagcggg ttctgcggtc
     3721 accgttaaac aggcaccttt aatctggtaa tgcgcggcgg taaaattatg attgttggta
     3781 ctgtacccgg cgattcggca atcaatttcc tcaaaatcaa tcgcgaagtc actatccaga
     3841 cggtattccg ctatgccaat cgttatccgg tcacgattga agctatttct tcagggcgat
     3901 tcgatgtgaa atcgatggtg acgcatattt acgattatcg ggatgtacaa caggcatttg
     3961 aagagtcagt taacaacaaa cgcgacatta ttaaaggcgt tattaaaatt agcgattaat
     4021 taatacggga gaacagaaca tgctcgcaga tatcagatat tgggaaaacg atgccacaaa
     4081 taaacattat gcaattgccc attttaacgt atggaatgca gaaatgttga tgggcgttaa
     4141 agacgcagcc gaagaagcga aatccccggt tattatttct tttggtacag gttttgtcgg
     4201 taacacctca tttgaagatt tctctcacat gatggtatca atggcacaaa aagcaacggt
     4261 gccggtaata actcattggg atcatggtcg gagtatggag attattcata acgcctggac
     4321 tcatggtatg aattcattaa tgcgtgatgc ttctgcattt gatttcgaag aaaatattcg
     4381 tttaaccaaa gaggctgttg atttctttca tccgctgggt attccggtag aggcggaatt
     4441 agggcatgtc ggtaatgaaa ccgtttatga ggaagcgctg gcgggctatc actataccga
     4501 tcctgaccag gcggctgaat ttgttgaacg tacgggctgt gactcattgg ctgtcgccat
     4561 cggcaaccag catggggttt atacgtcaga gccgcaattg aactttgagg tcgtcaaacg
     4621 cgtacgcgat gctgtttctg ttccgctggt tttgcatggc gcatcgggga tcagtgatgc
     4681 cgacattaaa actgcaattt cgttgggtat tgcgaaaatc aacattcata cggagctctg
     4741 tcaggccgca atggtcgcag tgaaagaaaa ccaggatcag cctttcctgc atttagaacg
     4801 tgaagtacgt aaagctgtaa aagaacgggc attggaaaaa attgaactgt tcggttctga
     4861 tggcaaagcg gaataatgat atggataatc tcgacgttat ttgtataggt gccgctattg
     4921 ttgatattcc attacaaccg gtcagtaaaa atatctttga tgtagattct tatcctcttg
     4981 aaagaatcgc aatgagcacc ggggggcgat gcaataaatg aagcaacaat tatttctcgt
     5041 ctggggcatc gtaccgcatt aatgagccgc attggtaaag atgcctcagg gcaatttatt
     5101 ctcggccatt gtcgcaaaga gaatattgat attcaaagcc tgaaacagga cgtcagcata
     5161 gatacatcta ttaacgttgg actggtgacg gaggatggcg agcggacgtt tgtcacgaac
     5221 cgtaacggca gtttgtggaa attaaatatt gacgatgttg attttgcgcg gtttcctcag
     5281 gcgaaattat tatcactggc cagtattttc aacagtcctc tattggatgg taaagcgcta
     5341 acagaaattt ttacgcaagc gaaagcccgg cagatgatca tttgtgccga tatgatcaaa
     5401 ccgcggttga atgaaacgct ggatgatatt tgcgaagcat tgagttatgt cgattatctg
     5461 tttccaaatt ttgccgaggc aaaattactc acagggaaag agacactgga tgaaattgct
     5521 gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa agacggctgc
     5581 tttatcaagc gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat aaccgccatc
     5641 gacaccattg gcgcgggcga taactttgct tcaggtttta ttgcggcact gttagaaggc
     5701 aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc ggttctaagc
     5761 gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg aacaattgct ggaagaatac
     5821 gaaggataat gaaggcaaat gaaaaagata cctttaggca caacggatat aacgctttcg
     5881 cgaatggggt tggggacatg ggccattggc ggcggtcctg catggaatgg cgatctcgat
     5941 cggcaaatat gtattgatac gattcttgaa gcccatcgtt gtggcattaa tctgattgat
     6001 cctgcgctag gatataactt tggcaatagt gaagttatcg tcggtcaggc gttaaaaaaa
     6061 ctgccccgtg aacaggttgt agtagaaacc aaatgcggca ttgtctggga acgaaaagga
     6121 agtttattca acaaagttgg cgatcggcag ttgtataaaa acctttcccc ggaatctatc
     6181 cgcgaagagg tagaagccag cttgcaacgt ctgggtattg attacatcga tatctatatg
     6241 acgcactggc agtcggtgcc gccatttttt acgccgatag ctgaaactgt cgcagtgctt
     6301 aatgagttaa aggctgaagg gaaaattcgc gctataggcg ctgctaacgt cgatgctgac
     6361 catatccgcg agtatctgca atatggtgaa ctggatatta ttcaggcgaa atacagtatt
     6421 ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc gtgataatgg cattgtggtt
     6481 caggtttatt ccccgctaga gcagggattg ttgaccggca ccatcactcg tgattacgtt
     6541 ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct gaaagtgatt
     6601 gatatgcttg aacagtggca gccactttgt gctcgttatc agtgcacaat tcccactctg
     6661 gcactggcgt ggatattaaa acagagtgat ttaatctcca ttcttagtgg ggctactgca
     6721 ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga tgcagacgca
     6781 acattgatga gggaaatggc agaggccctg gagcgttaaa tactcaacac gattcacctc
     6841 tgtttctgtg aaaactgtga tgaaaatctg tgattcaaat aggttatttt gaaagtaaac
     6901 atcggttatg cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg taatcgtggc
     6961 ggcaaaagac aggattcagg caattaagca aatggttgcc aacgataaaa aggtgacagt
     7021 ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc attcgccgcg atcttgagaa
     7081 gctggaagat gaaggctttt tgaccagaac ctatggtggt gctgttttaa atacagcggt
     7141 gttgacggag aatatccatt tttataagcg cgcttcatcg ttttatgaag agaagcagct
     7201 tattgcacgc aaggcactac cctttatcga caataaaacc accatggcag ccgattccag
     7261 tagtaccgta atggaattgc tcaaattgtt acaggaccgt agtgacctga cgttgctaac
     7321 caactcggcg gaagcgattc atgttctggc tcagtcagaa attaaagtcg tttcaacggg
     7381 tggggaacta aacaaaaata cactttccct gcaaggaaga attactaaag agatcatcag
     7441 ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt cttgatatta acagcggcgc
     7501 gctggactct aatgaagcgg aagctgaaat caaaaagaca atgatccgtc aggcgacaga
     7561 agttgcgtta ttggttgatc actctaagtt tgatcgtaaa gcttttgtcc agttagctga
     7621 ctttagtcat attaattata taataactga taaatcaccg ggtgcagagt ggatagcatt
     7681 ttgcaaagac aataatatcc agctggtgtg gtaatttaag cacacgttgt caatattatt
     7741 tgttcctgcg cctggaaata aaatgatgat aaagatgcgg caagtgaatt tgccgtaatt
     7801 gctacctttt atagcaaatc cgaggcaccc aatggaacaa tatgatcaaa ttggcgcaag
     7861 actggaccgc ttgcctttgg cccggtttca ttatcgtata tttggtataa taagctttag
     7921 tctgttatta acggggtttt tgagttactc aggtaatgtc gtcttagcaa agctggtaag
     7981 caatggatgg tcaaataatt tcctcaatgc cgcctttacc tcggcattaa tgtttggtta
     8041 tttcatcggc tcacttactg gtgggtttat tggtgactac tttgggcggc gcagggcgtt
     8101 tcgcataaat cttctcatcg tcggtattgc tgcaacaggg gccgcttttg tccctgatat
     8161 gtactggctc attttcttgc gcttcctgat gggaacagga atgggggcgc tgattatggt
     8221 tggctatgcc tcatttacgg agtttatccc cgcgacggtg cgtggaaaat ggtccgcgcg
     8281 gctctcattt gttggtaact ggtcgcccat gctgtctgcg gcgataggcg tggtggttat
     8341 cgcttttttt agttggcgaa taatgtttct tctgggtggt attggcatac tgttagcctg
     8401 gtttctctca ggtaaatact ttatcgagtc gccacgatgg ctggcaggga aagggcaaat
     8461 cgccggtgca gaaagccaac ttcgtgaagt agagcagcaa attgaaaggg agaagagtat
     8521 tcgtttaccc cagcttactt tgaaccagag caacagcaag gttaaggtaa tcaagggtac
     8581 cttcaggctc ctgtttaaag gggaaatgtt acgacgtaca ttagttgcga taactgtttt
     8641 aattgcaatg aacatttcgc tttataccat taccgtatgg ataccgacca tatttgttaa
     8701 ctccggcatt gatgtcgata aatcaatatt aatgaccgct gttattatga ttggtgctcc
     8761 ggtaggaata tttattgcgg cattaattat tgatcatttt cctcgtcgat tatttggctc
     8821 cgccttactt attattattg ccgtgttagg ctatatctat tcaagtcaga ctacagagtg
     8881 ggcgatttta atctatggtc tggtgatgat cttcttttta tacatgtatg tttgcttcgc
     8941 gtcggcggtt tatatcccgg agctttggcc aacgcattta cgcctgcgcg gttcgggttt
     9001 cgttaatgcc gtcggacgga tcgtcgcagt tttcacgccc tatggcgttg cggcattatt
     9061 aacacattat gggtcgatca cggtgtttat ggtgcttggt gtcatgttag tgctctgtgc
     9121 gctggttctc tccatttttg gcatcgaaac gcggaaggtg tcgctggaag agatttctga
     9181 ggtgaattaa atctttcgtt gatggcatca attgccagat gtaccactga cgtgttttat
     9241 ttggcctaca atgccagtac agaattgtag gccagcgtaa aattacccct gcgtctcttc
     9301 ccagtctgcc agcgtatata gcgttgcccc agctgctgac atctccataa acgcgtgtgc
     9361 actgtcctgg ggctggatat tcacgccacg acagccatcg gtaatcacgt ttaccttata
     9421 acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag ccaggcccat
     9481 aacgatcaat tcatcgattt catgatcgcg caaccagtca tcaagcgcgg ttttctgccg
     9541 acgtccgtta tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc ctttatggaa
     9601 caccgctgcg atctcttttt gattcagtaa cggatgtaat tgtgcgcctt cactgttctg
     9661 cacacagtgt tcaggccaga aagtttgccg caggccgtcg agttggcctg gcgtataagg
     9721 ctctacgccg tgctggctgg caaagctgcc gtgattcgcc ggatgccagt cctggctggc
     9781 gataaccgct tctccgcgcg actggcacca gtcaataagg cggttagcaa cctccaccgt
     9841 actgtcacct tccggtacgg cgagcgcgcc accagcacag aaatcatttt gtaaatcgac
     9901 cagtaacagg gcgcgatggg gcattgcagt ctccttaatc atccggcgtc agttcgccgc
     9961 gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc cagttcctgg ctcagcaggt
    10021 aatgcagttt ggttagcgtg gcttctacag tcatatctgc accgccaata acgccggcat
    10081 gggcgaggac gttaccggtg gcataaccac tcatattcac tttaccggac atacattgtg
    10141 tcaggttgac caccacaata ccgcgatcgc tggcttcttg taattcctgc aggaaggctt
    10201 tgttttgtgg cgcattaccc acgccatagg agcgcagaat caatgctttc accggttggc
    10261 gcagaaaatt gcgcacgacg tcagcagaaa tccctggata aatcgtcact acgccaattg
    10321 gttgtggcgt gattggatga acgattaatt ccccttcacc gtgcggggcg ggtggcgtat
    10381 tcaaacgacg aatatggata ccagcttcca gtaacggagg aaggtttgga gaggcaaacg
    10441 catcaaaacc atcggcatgg gctttggtag tgcggttgcc gcgatacaat cggttattga
    10501 aaaagagcgt tacttcgttg atcggataat tcgccgcaac gtacaacgca ttcagcagat
    10561 taatttgtcc gtcagagcgt aactcagcca gcgggatttg tgaccctgtc acaatgaccg
    10621 gtttaccgag attctcgagc atgaacgaca gcgcagaggc ggtatacgcc atcgtgtcgg
    10681 taccgtgtag aatgacaaaa ccatcatagt cgtcatagtg cgctttaata tcttcagcaa
    10741 tatgctgcca gtcttccggc gtcatatcag aagaatccat caacggcgta tattcatgaa
    10801 tggtgaaatc tggcatctcc aggcgatgga attccggcat cagcgccagt tggcgttgta
    10861 gatgacctga caccggaata taaccctgct cggaacgctg catcccgatg gtcccgcccg
    10921 tgtaggcgac gtaaattgat ttcttttgca tgatattgat atgaatgata tcgaaagagc
    10981 aaaagtatat acgtgaggca ggaggagcgc acctgatact atgcggataa ggaaatatgg
    11041 ggcgagacag cgggaagagg attcgtcgag gtaaaaaata atgtggccga cgatctgtcg
    11101 gccactcagt acaagactta acgcacgttg gcgcaggtca ggcaaaacgc ataacggttt
    11161 tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt taacggtaga ggctaccgag
    11221 gcaagcggtg caggtaacat ggcctggaac gcatctggca acattgcccg gacagaacca
    11281 gacatgttgt ccatcacttt gtcgaagaag gtcggttcat caacgtagta ttccagatgc
    11341 cactgtttca cttttgcgag ctctgctgct ttggcaaccg catcatcgaa atccccgaga
    11401 ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc ctgggcgatt
    11461 ttatcaattt gctccggcgt cgaatgacgc gcatcagcaa ccagcgtgat aaagcgttta
    11521 tagccattct caatgcttaa ttgcatcatc tgctgcgctt ccggcggcag tgccctggtg
    11581 atagaaacat ccgccagcgg tgaagttgag acaccatcag tatgaacacc aatcgaatcc
    11641 agactatttt ctacggtggt gatcacgccg aagataccga tagaaccggt cagggtgctg
    11701 gggttagcca caatgtaatt agctggcgtg gaaatccagt aaccaccaga tgccgccatg
    11761 ccgcccatcg atacaaccac agacttaccc gctgcccggg ctgctgccag ttcagcgcga
    11821 atcacttcag acgcggtaac gctgccgcct gggctattaa cacgcaggac aatcgctttc
    11881 actttcgggt caaggcgagc gtcgcggatt tgtgccgcag tggtatcacc gccaacattc
    11941 ccctgagttt cctcgccatc cataattgcg ccattagcga agacgacacc gatgctgtca
    12001 ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg ataattttta
    12061 tcagtcttac tccagccgaa ctctttggtc agagcttttt cgatttcggc actcgatgcc
    12121 agtgcatcga ccagcttgtt ttccagtgca tatttcgcgg tatcgccacc ggttttggtt
    12181 aaaccctcaa gcaacccttg cgcgccaggg aatacctgct gagcagggat ctgccggtta
    12241 gcggcaacag tattcagata gttttgccac agctcaccaa tccagcggct gtcagcttcg
    12301 cgggctgccg gtgacatatc atcacgaata aacggttcaa cggcagattt atacgtaccc
    12361 acgcggaaca catgggtgga aactttcagc ttatccagca acgatttgta gtacagaccg
    12421 ttggtagcaa aaccgtgcag atcaaccacg ccttgcggtg acagccaaat tttattggcg
    12481 aaactggcga gataatattg cccctggctg tagttctcgc caacggcata aaccggtttc
    12541 ccgctgtcac gaaactcttt cagggctttg ccgatgtact gcatagacgg ctggtcgccg
    12601 cctgcgaagt ttttcagatc catcacgata ccggtgatat tgcggtcgtc cttcgcctgg
    12661 cgaatggtgt tgacgatatc aaacagtgag ttttcctgca gacgatcgga actggcacca
    12721 agcagctggc ggcttaattt actaaaccgc tgagaactgt ctggtttatc gacgatcaca
    12781 ccagaaatgt ccagcagcag tgcgccacga ctggccgttt ctttcgaatc accaccactg
    12841 acctgcatcc agatccccac accaaccagt acgaggaaaa taaagaacag gttaagtacc
    12901 atttcacgga cgaaattcag cagacgccac gtccatttaa aaaatccggc aataaatcgc
    12961 caaagggttc gcatgtattc tcccaactta aggtcacacc tgtcacaggc gcaatagatg
    13021 tggttatcgt aatgacccaa ccggcacttg tcagcaggaa ttgcggactg tgctgtaaca
    13081 aaatccactc tcgtgttaat tttgtgaata aatatcacga caggagttaa tcaaatggat
    13141 gcactcgaac tattgatcaa tcgccgtagc gcctcccgct tggctgaacc cgcgccaacg
    13201 ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca taagtccatg
    13261 caaccgtggc atttttttgt gattgaaggg gaagggtgcg agcgtttcag cgccgtactg
    13321 gaacaggggg cgattgctgc cggtagtgat gataaagcta tcgacaaagc ccgtaatgcg
    13381 ccgttccgcg caccgctcat catcacggtg gtggcgaaat gcaaagaaaa tcataaagtc
    13441 ccgcgatggg aacaggaaat gtctgccgga tgcgcggtca tggcgatgca aatggcagcc
    13501 gttgcccagg gatttggcgg catctggcgc agtggcgcat taactgaaag tccggtagtg
    13561 cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg gttttctcta cctcggcacg
    13621 ccgcagttga aagcatctac gtcgattaac gtcccggacc cgacgccgtt tgtaacttat
    13681 ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa ttcagacact
    13741 ctcacttatc acttcacgga atgagggtta ccatacccgg attgcattga caggagatgt
    13801 ccatgagcga gaactcgatt cgtttgaccc aatacagcca cggagctggt tgcggctgta
    13861 aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag tttgttgatc
    13921 cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat ctgggcaatg
    13981 gcaccagcgt tatcagtacc accgacttct ttatgccgat cgttgataat cctttcgatt
    14041 ttggccgcat tgcggcgact aacgccatca gcgatatctt cgcgatgggg ggcaaaccga
    14101 ttatggcgat tgcgatcctc ggctggccga ttaacaaact ttccccggaa attgcccgcg
    14161 aagtaaccga aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg gctggcggtc
    14221 actccatcga tgcgccggag ccgatttttg gtctggcggt aacggggatc gtaccgaccg
    14281 agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa actgttcctg acgaaaccgc
    14341 tggggatcgg cgttcttacc acggctgaga aaaaatcact gttgaaacca gaacatcagg
    14401 gactggcgac ggaagtgatg tgccggatga acatcgcagg cgcgtccttt gccaacatcg
    14461 aaggcgtaaa agcgatgacc gacgttacgg gctttggtct gctgggccac ttgagcaaaa
    14521 tgtgtcaggg ggctggtgtg caggcacgcg tcgactatga agcgatcccg aaactccccg
    14581 gtgttgaaga gtacattaag ttgggcgcag tacctggcgg cactgaacgt aactttgcca
    14641 gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg cgatctgctg tgtgatccgc
    14701 aaacttctgg cggtttgctg ctggcggtca tgccggaagc agaaaatgag gttaaaacta
    14761 cagccgccga gtttggcatt gaactgacgg cgattggcga actggtgcca gcgcgcggcg
    14821 gtcgtgccat ggttgagatc cgttaattca atgcggttgt ttattgccga aaaaccgagt
    14881 ctggcgcgcg ccattgctga tgtcctgccc aaaccgcacc ggaaaggcga tggctttatc
    14941 gagtgcggta atggtcaggt ggtgacttgg tgtatcggtc acctgcttga gcaggcgcag
    15001 ccagacgcct acgacagccg ctatgcgcgc tggaatcttg cggatttgcc gattgtcccg
    15061 gaaaagtggc aattacagcc acgaccctcc gtgaccaaac aacttaacgt catcaaacgc
    15121 ttcctgcatg aagccagcga aatcgttcac gccggggacc cggatcgtga agggcaattg
    15181 ctggtggatg aagtgctgga ctatctgcaa ctggcaccgg aaaagcgcca gcaggtgcag
    15241 cgttgcttga taaacgacct gaacccgcag gcggttgagc gggcgatcga ccgtcttcgt
    15301 tccaacagtg agtttgtgcc attgtgcgtt tctgcgctgg cgcgagcgcg tgccgactgg
    15361 ctgtacggca tcaatatgac ccgtgcgtat accattctcg gtcgcaatgc cggttatcag
    15421 ggcgtacttt ccgtgggacg cgtgcagaca cccgtgctcg ggctggtggt gcgccgcgat
    15481 gaagagattg aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca tatcgtgaca
    15541 cctgccgatg agcggtttac cgctatctgg caaccgagcg aagcgtgtga accgtaccag
    15601 gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa ccgcattagt
    15661 ggtcaaccgg ctattgtcac cagctataac gataaacggg aatcagaatc cgcgccgctg
    15721 ccgttttcgc tttcggcgtt gcagattgaa gcggcaaaac gcttcggtct gagcgcgcag
    15781 aacgtgcttg atatctgcca gaagctgtac gaaacgcaca agctaatcac ttatccgcgt
    15841 tccgattgtc gctatttgcc agaagaacat tttgccggac gccacgcggt gatgaatgcc
    15901 atcagcgttc atgcaccgga tctgttgccg cagccagtgg tagatccaga tatacgcaac
    15961 cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca tcatcccgac cgcacggagt
    16021 tctgcgatca acctgacgga gaacgaagcg aaggtctata acctgattgc ccgtcaatac
    16081 ctgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaaca ggacattgcc
    16141 aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag caggctggcg cacgctgtta
    16201 ggcagcaaag agcgcgatga agaaaacgac ggcacgccat tgcctgtggt ggcgaaaggc
    16261 gatgagttgc tgtgtgaaaa aggtgaagtg gtagagcggc aaacccagcc gccgcgccat
    16321 tttaccgatg caacactgct ttcggcgatg accgggatcg cgcgctttgt gcaggacaaa
    16381 gatctgaaaa aggtccttcg tgcgaccgat ggtctgggga cagaagcaac ccgtgccggg
    16441 attattgaac tgctgttcaa gcgtggtttt ctgaccaaaa aagggcgcta tatccactcc
    16501 accgacgccg gaaaagcgtt attccattcg ctgccagaaa tggcgacgcg accggacatg
    16561 accgcgcact gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg ctatcaggac
    16621 tttatgcagc cgctggtggg gacgctatat cagcttattg atcaagccaa acgtacgccg
    16681 gtgcggcagt ttcgcggcat tgtggctccg ggcagtggtg gcagtgctga taagaaaaag
    16741 gctgcaccgc gtaaacgtag tgcgaaaaaa agtccgccag cagatgaagc cggaagcggg
    16801 gcgatagcgt aagcgagcga atctttcgtg ctattcgagt catattctga aatatccagc
    16861 ggatcaagaa aattcgttgg atattttttt gcatggataa aattatcgcc tctaaagtat
    16921 gtaataacag ggaatgtgat gaaaaaagtt ttattacaaa accatcctgg gagcgagaag
    16981 tattctttta atggctgggg aatatttaat agtaattttg aacgggtgat taaagaaaat
    17041 aaggccatgc tgctttgtaa gtgggggttt tatttaacat gtgttgtcgc tgtaatgttt
    17101 gtattcgcag cgataacatc caacggtttg aatgaaagag gcctgattac cgcgggatgc
    17161 tcttttcttt atctattaat tatgatgagg cttattgttc gggccggttt taaagcaaaa
    17221 aaagaacaac tacattatta tcaggctaaa ggtattgagc cgctcagtat cgagaagtta
    17281 caggcgctac aattgatcgc accttatcga ttctatcata agcaatggtc tgaaacgcta
    17341 gagttctggc cgcgaaagcc tgaacctggc aaagatacct tccaatatca tgtgcttcct
    17401 tttgactcga tcgatatcat cagtaaaaga cgcgagtctt tagagaatca atggggtatc
    17461 gaagatagcg aaagttattg tgccttaatg gagcattttc tttctggcga ccatggagcc
    17521 aataccttta aagcaaacat ggaggaagcc ccagagcagg ttatcgccgt gttgaataaa
    17581 tttgctgttt ttccctcaga ctatatctct gattgcgcta ataatcgctc cggtaaatcc
    17641 tcggcgaagc taatatgggc ggcggaatta tcatggatga tctcgatatc aagcacagct
    17701 tttcaaaacg ggacaattga agaagaactg gcctggcatt atataatgct tgcttctcga
    17761 aaggcgcacg agttgttcga aagcgaagaa gattatcaaa aaaatagtct aatgggattt
    17821 ctttactggc atatctgctg ctatcgcaga aagttaacgg atgctgaact tgaagcgtgt
    17881 tatcgttacg acaagcagtt ttgggagcac tacagtaaga aatgccgttg gcccataaga
    17941 aatgttccct ggggagcatc atccgttaaa tactcataaa cgcctgaaat tttgccgggg
    18001 gcgctgcgct tgcccggcct acattagagc gtaactgcaa tgaattgtgc ccatctgtag
    18061 gcctgataag cgtagcgcca tcaggcattt acaacttaaa tcacaccctg cgccagcatc
    18121 gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt ggtttgctca
    18181 ccttcaccac catgctcaac acaggcatgg tggatatcca gcatgatgtg atgcaaacgt
    18241 gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat ttccaggccc
    18301 gacgttgcta caccgccagc attagccgct ttacccggtg caaacagtac gcctgcctgc
    18361 tggaacagtt cagtcgcttc gatggtggtc ggcatatttg ccccttcagc gacggcttta
    18421 acgccattgg cgataagctg atgcgcggtg tcaacatcca gttcgttctg ggtggcgcaa
    18481 ggcagggcga tatcaaccgg tacagaccac ggctgttggc cttcgagata gaccagacca
    18541 aattctttgg cgtaatctgc cactcgacca tcgcggctgg ctttgatttc gataagacgt
    18601 gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca
    18661 gtgatcgcac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc gacgttgccg
    18721 gagccagaaa cggaaacgcg catcccttca aaacccatac cgtggcgttt taacattgct
    18781 tctgtgaaat aaaccagacc gtagccggta gcttccgggc gaataagact accgccaaat
    18841 gaaaggccct taccggtgaa gacgcaggcg gtattgttgg agagcttttt catcatcccc
    18901 gccataaagc cgacttcacg accaccaacg ccgatatcac ctgccggaac gtcggtatcc
    18961 gcgcccaggt ggcgatacag ttcagtcatc agcgcctggc aaaaacgcat cactttacct
    19021 tcgctttttc ctttcggatc gaaatcgctg ccgcctttac caccgcccat cggcagggta
    19081 gtcagggcat ttttgaaggt ttgttcaaag ccgaggaatt tgagaatgga aaggttaact
    19141 gacggatgga agcgcatacc gcctttgtac gggccgatgg cagagctgaa ctgcacacgc
    19201 catgcacggt tgacctgtat ctggttgcga tcatcaaccc ataccacgcg aaactggatc
    19261 acgcgctccg gttcaaccag acgctccagt aatgacatct ggcgatattt tggattttgt
    19321 tcaagaaaag gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa ctcggtttga
    19381 ttcgggtcgc gcttttggac atggttgagg aatgactcca gagaatatgt ctgatccata
    19441 gatataaaaa ccttatatat taatacgatt gtgcttatgt tgtttatgtc atgtgctttt
    19501 gcagttttcg actataccat caagaatgtg acggtaagca agaaaaaaat gacccaggaa
    19561 agcaaattaa taacgagagt aatctcataa ccaaatgcga taactcagac gctaaagttt
    19621 tgccacccgg ctaccgttat cagtagcaga gacaataacg ttaaggagtt ccctgtgagt
    19681 cgagcattgt tcgccgtagt attagcattt ccattaattg cactggctaa cccgcattat
    19741 cgtccggacg tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg gcagtcagcg
    19801 cagccctgta ctcagtgctg cgtttatcag gatcaaaatt attctgaagg ggcggtaatt
    19861 aaagccgaag gaatactgct gcaatgccag cgtgatgaca aaacccttag cactaatccg
    19921 ctgctctggc ggcgcgtaaa gccataaacg cctctaataa tggaatgtca gcaggggcca
    19981 gcggatattg cagcgcctct tcaggtgagc accagaccag cgcctgatgt tcatgtgcct
    20041 gtaacgtccc gtggaagtcg ggtacgtgcc aggcatgaag atggataatc cggcccgaaa
    20101 cttctcgctg atggctggca acatactcac ccacagttgc ttcgatgtcc aattcttcgt
    20161 ttaactcacg caccagcgcc tgctgctggc tttcatccag ctcgacttta cctccggcaa
    20221 actcccataa tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa attttgccat
    20281 cacgttcaat gatggcggca acaacttcaa tcattttcat aaattacaca cgggcagata
    20341 agcgaaaaat gccataatat ccgcactgtt gctcaatcag aaaggcttta tatttaagga
    20401 gtgcctgtgc tagaccgcca tcttcatccc cggattaaac cgttgttgca tcagtgcgtg
    20461 cgggttcttg ataaatcggg cattacgcca gatggtttaa cattagtggg atttgccatc
    20521 ggcgtgctgg ctctgccgtt tctggcgctg ggctggtatc tggcggcgct agtcgtcatt
    20581 ttgttgaaca ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag agggcttacc
    20641 gacgcgggcg gctttcttga tatttctctc gattttctct tttacgcgct ggtgccgttt
    20701 ggctttattc ttgctgcacc ggagcaaaat gcgctggcag gcggctggtt gttgtttgcg
    20761 tttatcggca cgggcagcag ttttctcgcc tttgctgcac tggcagcaaa acatcagatt
    20821 gataaccccg gttatgcaca taagtcgttt tactatctgg ggggattaac cgaaggcaca
    20881 gaaacgatct tactgtttgt gctgggatgt ttattccctg catggtttgc gtggtttgcg
    20941 tggatcttcg gtgcgttgtg ctggatgacg acctttacgc gggtgtggag tgggtatctg
    21001 acgctgaagt cgctccagcg tcagtaattg caatgttatt tgctactgcc cgggccgcgt
    21061 tcaccggttg caaccggatt ttttggatcg ctgctccatt cgtaccagcc gccgtcatac
    21121 acggaaacat tcttccaacc catggcgcgt gcgtacataa aggtttcgga cgcgcgccag
    21181 ccggtgccgc agtagaatga aacttgctga tctggtttga tattccatgc tttccacata
    21241 gcggtaatat catcggcgct acgcatggtg ccatccgggt tatggaaatc ttccatatgc
    21301 gtcgagtcgc taccagcgtg tccccaacgt gctccggcta tttcaccttt tggtttaata
    21361 tagctgtaac cgctggtcgt accgataaat tctggccacg aacgaatgct caccagcgat
    21421 gcatcctggc gatgcagcag tccacgggcc tgttccatat cgagcatcaa ctgcggttgt
    21481 gccgggatct tcacgccgaa atctggttcc gctttcactt tcggtggcgt tcctcgctca
    21541 acaggcagtc ccgcgtcgga ctaggtttgc cagccgccat ccagcagacg cacatctttc
    21601 acgcctgcgt agagcataat ttgcgccaca cgcgctccag cgtatacgtc acgcccgtac
    21661 agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca gcatcgcttt tagttgttca
    21721 tcagaaactt tgttccacag cggttcgctt tccacttcgt tggtatcgat gtagtcagcg
    21781 ccaggaatat ggctgataag gtaaagctta ggagcgcccc aggccgcttc aatgactttc
    21841 cagccaccga caggtttcgc cgtaacctct ttaccttgtt gcaggtcatg cagccattgc
    21901 ggataaacca gctgctcaaa gtgcggcagt ttttgtagac gggaaggttc gcttagcgtg
    21961 tcactcagaa tggagatatg cgtaaaacct gctttttgca gtcgcgtttt gacggcgtcg
    22021 acatctttgc cattaccgta cagcgccacc ggggcatcgg ctttcaggtt atgttgcttg
    22081 atccacgcgt tgagctgttc ggtgctcatt ttgtcgagcc agctagcaga gaggtttaag
    22141 gcggcaggtt catgaccaga agggccattt aaggtttgtg gccagccgtt ataaaacgcg
    22201 ctggggcgag tatctatcgc tttgccattt tgttgctgaa gttggtcaag tgtaagaggc
    22261 ttcgccagtt cagcggccca cgaagaagcg caagccagcc ctaaagccat tgccagcgcg
    22321 gtcatttgag aaacacgttt catcgaaaat cccgacatta tagttttgag tgcgccattc
    22381 tgaactgacg cattgagaaa aactttgcgt tatcgcagtt tgttgtaatt ttctgaccac
    22441 tgcgccatat ccagaacaga actatcagca ggaacatcct ggagatcgtg cgttacctga
    22501 acgacgggga tcgccagggc gcgaacttcg ctgaacaccc actggcgaaa attatcgcgc
    22561 agagccacat caagacggct gaatggctca tccaggagta acgcttttgg ctgggcgaga
    22621 agggcgcgta gtagagcaac gcgcgctcgc tgaccgccag acaaagtggc aggatcctga
    22681 tggaaagctc cgtcaaggcc tgaacgctca agtgcatcat tcacggcatt acgtcgggca
    22741 ttccccttaa gtgtcgccgg tagcgccagc agtaaatttt gcccgacgct gaactggtca
    22801 aataacagtg catcctgaaa aagaatgcca atctgacgct gtgcggtggg tagcatgtca
    22861 atccgttgct cattgagcca tagctcacct gtacaagaaa actgtccggc cagtgcacca
    22921 atcatccatg aaaacagagt ggattttcca cagccagatg gccccattaa cgtgacgatg
    22981 tcacctttat caaccgtaaa gttaacgttt gtcagcaagc ggctttctgg taaacgtagc
    23041 gaaacatttt tcacgcagag cattagcgga ttccttgtct gacataacct acccattttg
    23101 cgactaacgc ggtcagggca aaaataataa gcggtaacaa cagttgccat aaagcctggg
    23161 cggcgagaat accgttgctg ccgccgctgc ttaatgccac cgcctcagtg gtgagcgtcg
    23221 gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg cgcaatactg actgaaaatc
    23281 ccaccgcgaa ggcaatcagc acagggcgca acatgagtgg gcatttcacg taaaagaaga
    23341 ttttggcccg cgaccagccc agtgtttgcg caatcaaaat taaccgtgaa tcaatgcgct
    23401 gccaggcggg ttgcaggata aacagcatcc acggcattac ccacagcaga tgcccccaga
    23461 ccaccgccgt ccagcttcca tccaggttca gccatagcgc cagcgtgtac tggcccgcca
    23521 ccagtggcag agcgggcaat agaatgggca accatagcca caactggcga cgctgtgccc
    23581 cccattccag ccacagcagt aacaggagca aagcgatgaa tgttgctacc agccccattg
    23641 tcagactgtt gatgagtgct tcactattga tcgtcgactg atccgcgagg atcgccagca
    23701 gaaccacaca cagcacaccg gttaagggta aaaaactcgc cagcgtagtg cctggtaata
    23761 aaggtgtggc aggcttgcga atgccatcta cgcggggaat agtgcgccgc cagctgcgcc
    23821 atagcagata gctcagcaaa acgtaggcgg cgagtaacag catcagcagc agactggcga
    23881 gtgcgccttt cgtttgttga tccgcgtcgc cctgagttaa ccactgccag ctaattactg
    23941 ccagcgtcgg tggattacct ggcccaagaa taatcgccac atccacgacc gaaagcgacc
    24001 aggcaacaat cgccagcatc gccattgcca gcgaaggcgc gacggagggc aacagcagcc
    24061 agttcaggca ttgccagcgg ctgtagccca gcgaatccag cacaatgacc tgctgcaaca
    24121 gccgtttttc gctcaatact gccactaaga tccacagcag aaatgcgctt tctttcactg
    24181 caagggtgag gcccagaccg atccaaatcg gtccattggc ggagtgaaat acgggaaata
    24241 gtcatatagc agccctccgt cagcaaagag cagcagggcg ctggtggcaa aagccacatg
    24301 gggaatggcg agcagccacg gcagacgggc gcacatacgc tgccatttcg gcccaggcca
    24361 tagcgccaca atcaccagca gggcaatcaa caatgccccg accgccacga tggttgtcga
    24421 caccagcgtt gccagtaatg cctgcggtaa ctgtggatcg gcaaataacg cctgccagcg
    24481 tgtcaacgac aacgcaggcg atatcagcgt gagagctgcc gggatcagcg gtgcataaat
    24541 caccgccacc atcgcccaca gcaaaaagat taatgcgtac cgtaacggcg taaccattct
    24601 tgttccagtg cattcaccca tcctgcgtgc ggttcagcca gtaccggcgg cagatcctgc
    24661 ggcattcttg attgcaatat ttcgcgctgc ccgtcaggca gtttttgcgg atcgagaaca
    24721 gaaggatcgc cccagacaac gggatctgct ttacgcagtt gcgcatcggg tgagagcagg
    24781 aaattggcaa ctaccttcgc cgcagcactg gcattcgcgt tggctggaat ggtgacgaaa
    24841 tgcacgttgc caatcatccc ctcgcgaaag ccaaaactgt aactgcttgc cggcaaatcg
    24901 ccgctggcga ttttttgctg cgcatgcgcg gggttaaagg tcagcgacag gcgcaatgtg
    24961 ccggctttca gcagagcatc catccgcgcg ggcgaaggcg ggaaatcctt tccttcgcgc
    25021 cacaaatacg gatgcagtgc atcaagatat tgccacaagg gcgcagtgac acgggcgaaa
    25081 gtcgcatcgt ccggcgcttc ttttaatgcg gcgggatctg gcgtcagcat aatcagcaac
    25141 tgttcaagaa acgccgtgcc ggtaaagtcc ggtgggcgcg gataagtaac cgtgccggga
    25201 ttggctttag caaactccag taaggcttgc ggcgtttgtg gtggctgtgg taaacgtcag
    25261 ttgtgcgccg ccccagggcg attccgcacc ttgtgtcggc actgaaaaat cttcccgcac
    25321 cggcagctgt gtgtcgacat agcgccagtt gggcagagtc tccgcccagc ccgtttgtaa
    25381 taaattggcc tctttta
//
LOCUS       DADLXX010000047        25100 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLXX010000047 DADLXX010000000
VERSION     DADLXX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.047"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(129..1649)
                     /locus_tag="JNX27_001930"
     CDS             complement(129..1649)
                     /locus_tag="JNX27_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7883158.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKSKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            1740..2471
                     /gene="tonB"
                     /locus_tag="JNX27_001931"
     CDS             1740..2471
                     /gene="tonB"
                     /locus_tag="JNX27_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7883159.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRAL
                     SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWR
                     YEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(2511..2909)
                     /gene="yciA"
                     /locus_tag="JNX27_001932"
     CDS             complement(2511..2909)
                     /gene="yciA"
                     /locus_tag="JNX27_001932"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7883160.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3014..3553)
                     /locus_tag="JNX27_001933"
     CDS             complement(3014..3553)
                     /locus_tag="JNX27_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7883161.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3583..4326)
                     /locus_tag="JNX27_001934"
     CDS             complement(3583..4326)
                     /locus_tag="JNX27_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7883162.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4354..4443)
                     /locus_tag="JNX27_001935"
                     /pseudo
     CDS             complement(4354..4443)
                     /locus_tag="JNX27_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4683..5321
                     /gene="ompW"
                     /locus_tag="JNX27_001936"
     CDS             4683..5321
                     /gene="ompW"
                     /locus_tag="JNX27_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7883163.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5381..5887)
                     /locus_tag="JNX27_001937"
     CDS             complement(5381..5887)
                     /locus_tag="JNX27_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7883164.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(5933..6433)
                     /locus_tag="JNX27_001938"
     CDS             complement(5933..6433)
                     /locus_tag="JNX27_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7883165.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6519..6698)
                     /locus_tag="JNX27_001939"
     CDS             complement(6519..6698)
                     /locus_tag="JNX27_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7883166.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(7079..7885)
                     /gene="trpA"
                     /locus_tag="JNX27_001940"
     CDS             complement(7079..7885)
                     /gene="trpA"
                     /locus_tag="JNX27_001940"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7883167.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(7885..9078)
                     /gene="trpB"
                     /locus_tag="JNX27_001941"
     CDS             complement(7885..9078)
                     /gene="trpB"
                     /locus_tag="JNX27_001941"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7883168.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(9090..10448)
                     /gene="trpCF"
                     /locus_tag="JNX27_001942"
     CDS             complement(9090..10448)
                     /gene="trpCF"
                     /locus_tag="JNX27_001942"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7883169.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(10452..12047)
                     /gene="trpD"
                     /locus_tag="JNX27_001943"
     CDS             complement(10452..12047)
                     /gene="trpD"
                     /locus_tag="JNX27_001943"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY7883170.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12047..13609)
                     /gene="trpE"
                     /locus_tag="JNX27_001944"
     CDS             complement(12047..13609)
                     /gene="trpE"
                     /locus_tag="JNX27_001944"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7883171.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            13882..14763
                     /gene="yciV"
                     /locus_tag="JNX27_001945"
     CDS             13882..14763
                     /gene="yciV"
                     /locus_tag="JNX27_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7883172.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            14760..15380
                     /locus_tag="JNX27_001946"
     CDS             14760..15380
                     /locus_tag="JNX27_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7883173.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15481..16353
                     /gene="rluB"
                     /locus_tag="JNX27_001947"
     CDS             15481..16353
                     /gene="rluB"
                     /locus_tag="JNX27_001947"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7883174.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16393..16983)
                     /gene="cobO"
                     /locus_tag="JNX27_001948"
     CDS             complement(16393..16983)
                     /gene="cobO"
                     /locus_tag="JNX27_001948"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY7883175.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(16980..17738)
                     /locus_tag="JNX27_001949"
     CDS             complement(16980..17738)
                     /locus_tag="JNX27_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7883176.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            17958..19007
                     /gene="sohB"
                     /locus_tag="JNX27_001950"
     CDS             17958..19007
                     /gene="sohB"
                     /locus_tag="JNX27_001950"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7883177.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     AWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19043..19294)
                     /locus_tag="JNX27_001951"
     CDS             complement(19043..19294)
                     /locus_tag="JNX27_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7883178.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            19674..22271
                     /gene="topA"
                     /locus_tag="JNX27_001952"
     CDS             19674..22271
                     /gene="topA"
                     /locus_tag="JNX27_001952"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7883179.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVIDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            22481..23455
                     /gene="cysB"
                     /locus_tag="JNX27_001953"
     CDS             22481..23455
                     /gene="cysB"
                     /locus_tag="JNX27_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7883180.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            23750..23914
                     /locus_tag="JNX27_001954"
     CDS             23750..23914
                     /locus_tag="JNX27_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7883181.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            23917..24084
                     /locus_tag="JNX27_001955"
     CDS             23917..24084
                     /locus_tag="JNX27_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883182.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            24198..24293
                     /locus_tag="JNX27_001956"
     CDS             24198..24293
                     /locus_tag="JNX27_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883183.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            24457..>25100
                     /locus_tag="JNX27_001957"
     CDS             24457..>25100
                     /locus_tag="JNX27_001957"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HAY7883184.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHT"
BASE COUNT         6337 a         6162 c         6338 g         6263 t
ORIGIN      
        1 cacagcctca gctaatgagc gagcttcaaa agtgcttaag tcgggtaatg ctttacggaa
       61 gtactctgtg aatccgaata agaagggcct tattgagaca taaaaagggg cttcatagcc
      121 cctgaaaatt acagttgata agcatcaata tccagtagct ctttatcagc aatttcaaag
      181 attggtgacc catcatttag cggtagcggg tctgtaggta tggtgatttc agtgtcgttc
      241 tgtagcgtta tagttactga acccttaccc aatgtaagcc cttttagatg tttggacaaa
      301 attaattgac actcgacacg acccgcttta gttgtcaggt caatatcttt aagctggggt
      361 agttccttat cagtgagtga agccttacgc ttcatagtat caatcgtggt ctgtagttct
      421 gtgcgcttct ggttaagttc cttaagctga atttttagtt catcaatatc atccatctca
      481 agaagtaggg cggtagcttt cttgattctg gctgtgacaa tttcaacctc atgctgtaac
      541 gctgcaatct tcctctctga ttctccatca ttccccacgt caatcatccc cagaaaatta
      601 tcaatcatcc atcggtcaaa cggtttacgc ttcaacggtg gaacatcaca gcgaccgtct
      661 aagtggttat tgcacctgta gaccccagca tacttactct ttgttccggt aggatggaca
      721 ctagccccac actgacacct aaacagacct ttgaagatgt taatcgttag tgggttgtca
      781 cttgcaggtg tacggccttt acgattctta tcaagaatct cttgagcttt attgaacgtt
      841 gcagcatcta tagcggcttc gtagtagttc ggtatagcgg ggcggtcatt gcgctgtgat
      901 gctggtagtg tacctatcgt tgtctctgac cttaagatag tacgcacaga atgaacgcta
      961 aatccattag catctttacg tcgtgagaat gaactaatgt tattagcctg taggtgacgt
     1021 gcaatgttat gcagactttc accatctagc agcagcttga aaatcaagcg tacagtttca
     1081 gcatgttcat tgagtttaaa agtctcaggg tcaatccatc cgggaacatt ccttgttaaa
     1141 cgttgttcac cagcaagggc aagctttgca cgttgttccc atttctgact aacacggaaa
     1201 gacttctgtt tactctcctg atgcgctatg tagcttttaa cccgctgaat gattgagatt
     1261 cctaaatcat cggatagagc atcccttgtg tagattagcc catcctgtag agtgtgaatc
     1321 tcgataccat gagcaataat gcgattcatc aaggcttctg tttcaagatg ttcaaggcgg
     1381 cctatgcggt cgatagcttc gactagtaaa attgtccctt ctggaaactc ccctgattca
     1441 atgcgggaga gcatatcccc aaattgccct gataagtgtt tacctttgta accggataga
     1501 ccttcatcat tgaaagatgc cactacctga gcatcgctat tcttccttaa ccactgtttg
     1561 aatagctcat tttgtcgtct ggttgaatca gcaccttcag caccttgtgt ggctgatgag
     1621 aatctcaagt atgttattac ggttctcacg gtgataaact cccatgcaat tgattatcat
     1681 ttaaagatga acttataaac gttatggcta atgctacagt gccgaatagc tacaatacaa
     1741 tgccccttga tttacctcgc cgcttcccct ggccgacgtt actttcggtc tgcattcatg
     1801 gtgctgttgt ggcgggtctg ctctatacct cggtacatca ggttattgaa ctacctgcgc
     1861 ctgcgcaacc aatttctgtc acgatggttg cgcctgctga tctcgaaccg ccacaagccg
     1921 ttcagccgcc accggagccg gtggtagagc cagaaccgga acctgagccg atccccgaac
     1981 cgccaaaaga agcaccggtg gtcattgaaa agccgaagcc gaaacctaag ccaaaaccga
     2041 agccaaaacc gaagccggtg aaaaaggtac aggagcagcc aaaacgcgat gtcaaacccg
     2101 tagagtcgcg tccggcatca ccgtttgaaa atacggcacc cgcgcgcccc acatcaagta
     2161 cggcaacggc tgcaaccagc aagccggtta ccagtgtggc ttcaggacca cgcgcattaa
     2221 gccgtaatca gccgcagtat ccggcacgag cacaggcatt gcgcattgaa gggcaggtta
     2281 aagttaaatt tgacgtcacg ccggatggtc gcgtggataa cgtacaaatc ctctcagcca
     2341 agcctgcgaa catgtttgag cgtgaggtga aaaatgcgat gcgcagatgg cgttatgagc
     2401 cgggtaagcc aggcagtggg attgtggtga atatcctgtt taaaattaac ggcaccaccg
     2461 aaattcagta agcagaaagt caaaagcctc cgaccggagg cttttgacta ttactcaaca
     2521 ggtaaggcgc gaggttttcc ttcaggatca accgcgacat acttaaataa tgcttctgtc
     2581 gctttatagc gttgcccgat tggttcagac gctacttttt tcacccacac ttcaatatta
     2641 atgctgaccg atgtcgtccc tttctggaca cagcgtgcat agcagcacac cacatcgccg
     2701 accgcaaccg gccgtaagaa agtcattcct tcaacccgca cagtcactac gcgaccgtgg
     2761 gcaatttctt ttgccagaat agcgccgcca atatccattt gtgacattaa ccaaccacca
     2821 aagatgtcac cattggcatt ggtatcggcg ggcatggcta aagtacgtaa aacaagatcg
     2881 ccctgaggga cgttatgtgt tgtagacatg gtaaaaccga cttaaaagga aaatcacagg
     2941 cgcgatgcta ctatgatttt cctgaggaga acagaggaga tacagcgcca gccccgaagg
     3001 actggcagtc tggttaggat ttatcttcct gcggcatgtg gcggtagata tagataccgc
     3061 ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa gactttaaag ttgacccaaa
     3121 tattttgcgg cagccagaat gcgatgtaga tgtttgccag accgcaaagg ataaagaaaa
     3181 cagcccaggc cagatttagc ttcgaccata ccggttgcgg cagcgtgagt tctttaccca
     3241 gcatccgctg aattagcggc tttttcatca cccattggct gactaacagg gctaccgcaa
     3301 acagagcata aatgactgta accttccatt taataaactc atcattgtgg aagaacagcg
     3361 tcaagccacc gaagacgacc accagaacaa aagtgatcag ggccatcttc tcaaccttac
     3421 gaaagcgaac ccagctatag ataagcacaa tcgccgtggc gacgatcagc gctgcagtag
     3481 ccgcatagat gtcataaatc ttgtaaaacg cgaaaaagac aaccagcggt aaaaaatcaa
     3541 gaaactgctt cattttacga ttccgtaatc aaatgcttaa ggttattggc gaatcaacat
     3601 atacaggcgg aacagataga tgagcaatat ggcagaaatc aggttgctca aggtgttcgc
     3661 cagtacggca ccaatttccg gggttaatgc ggcaaaagaa gaggcaaaaa gcagcaacag
     3721 tgtttttgcc agcaaccagc tcagtactgc gggtgccacc agacgcatat tcgcccaagt
     3781 cagccgcata ctgctacgca tcgaggcaaa aatgcccatt ttgtcctgta ccagcatcac
     3841 cggtgccagg gccagtaaaa tggccatgat aattcccgga acgaccacca gcatgatgcc
     3901 aatctgtact aaaagggtag tcagaaaaat cagaataaat aacttgggca atatcggcgc
     3961 actggcacca atagctcgca gcgcacagac tctctgaccc gcagacacca gctggataat
     4021 taatattacg cctccggcga gaatggcgtt accgattaat tctgaaaaag tggacgccgc
     4081 tgaagcctgc agcaaaattt gttgctgttc cggtgacata ttctgaacca ggtcgaacaa
     4141 cccactactg ccgctaacgg gcacgccgtc attgagctgc gcaagctgtg catcactggg
     4201 tgagaaaaca tgccctaaca ccactgtgat aaacgcacat agcaacgata ccaacagaat
     4261 ggtcataaat tgattacgga agaaatttcc ggtgtcacgg tatacggact gcgccgtgat
     4321 agacatgcac tctccttgag ttttatgcag gtgttaatta gcgggcaatt gtaccctggt
     4381 taagccttgg gtggcagcat caggtcttgt atggaaaagc atatctttgt aaagcggagg
     4441 taattgctgg ccttcaggcg tgggcaaacc gcggcaagtg gcgatcgagt taatatttgc
     4501 gtagagaaaa tatttaaaaa ttgatttaaa tcacattaac caggattctc aatgcaactt
     4561 ctaaattaat ccagatcaat aaatggtgaa ttatcatatg taatgtgatc tatgtaggat
     4621 catttgttac tccaatgtag gtatattcgt cacgttttta taaccataac gacggagcgg
     4681 atatgaaaaa gttaacagtg gcggctttgg cagtaacaac tcttctttct ggcagtgcct
     4741 ttgcgcatga agcaggcgaa ttttttatgc gtgcaggttc tgcaaccgta cgtccaacag
     4801 aaggtgctgg tagtacgtta ggaagtctgg gtggattcag cgtgaccaat aacactcaac
     4861 tgggcttgac gtttacttat atggcgaccg acaacattgg tgtggaatta ctggcagcga
     4921 cgccgttccg ccataaaatc ggcacccggg cgaccggcga tattgcaacc gttcaccatc
     4981 tgccgccaac actgatggcg cagtggtatt ttggtgatgc cagcagcaaa ttccgtcctt
     5041 acgttggggc cggtattaac tacaccacct tctttgataa tggatttaac gatcatggca
     5101 aagaggcggg gctttccgat ctcagtctga aagattcctg gggagctgcc gggcaggtgg
     5161 gggttgatta tctgattaac cgtgactggt tggttaacat gtcagtgtgg tacatggata
     5221 tcgataccac cgccaattat aagctaggcg gtgcacagca acacgatagc gtacgcctcg
     5281 atccgtgggt gtttatgttc tcagcaggat atcgttttta attccgcaca aaaacgaccc
     5341 cgtaatatac ggggtcaata aggacatggt ataaagggta ttatttcttc gcttctacgc
     5401 catcagtttc agaacgaatt aaaaatttct cagttgtttg cggaatatgc tgaatcagcc
     5461 agtcggccat ttgcttttcc tcatttaaaa tcgcttcgat gatcggaatt gagactgtat
     5521 caccggcatt ttttgctgct gctaatagtg aggtgtaaca ggcgatttca aattgctcga
     5581 agacatatcc gctaatagag cctttaacta tttcatcaga agggaatata ccaccgattg
     5641 actgcccaag cgcagccatt ttactcatgg aatctttaat gactgaacgt gaaatgtcat
     5701 tacgatcaag aatagtttcc agttgaacaa tctggttttt ggtttcacta agatgttgtt
     5761 caatacgagc gcgtagttca ggataattat ctatacggct ggccatggat tcaagcatag
     5821 attcggcttg cttttccatt gcgtgggcgt cacgtaacca gtcatgataa tgttcaatac
     5881 gattcatgaa aatgtcctct ttataaaatg actaaaagtt aaaattcata tttcaggctt
     5941 tattttcggc tttcttattt acgttattaa gggccagatc agtcagtttg atgtcggtgg
     6001 ccttttcttc ttccagggtt tctttcagaa gcttcgctgc tttacggtaa cctaattgtt
     6061 cagccagcgt cgctaatgtc ccgtaactgg caatctcata atgctcgact ttctgtgctg
     6121 cggcaatcag tgcggcatca cgcacttcgt ttttctcggt actttcgatg acctcattag
     6181 cttcttcaat aagaccttcc attgccacac atttcatgcg cttaattttc agatttgatt
     6241 ctgattccac aacttgatca attcgttcaa tctgtccatg agtttcctcg aggtgcgcat
     6301 gaaaagcctg acttaatttt tcatttgatg ttgctcttgc gagttttgcc agtgcccggg
     6361 gtaattgttt ttctgcgctg taggtatctg aaagcagttg aataaataca tcttcaatgg
     6421 tcttcatatt catatttttc tccagtgaaa tcactgcgag ccatacggcc cgcagatgca
     6481 attatcttta tcagctgata ttaataatta acggtgaatc aggatttgtc tgatttatta
     6541 ccaccgcttt gttgaccgcc ttttttaccc gcttcagatg cgcgttgcgg atcattttta
     6601 aaattaccgc cgctatgctg accgccttta cggcctgcgt cggatgcctt ctcacggtct
     6661 tcggcgaaat ttcctgaacc accacgatgt tcggccatgt tatttctccc gttgcgttgc
     6721 attgtttcat taatatgagt gttgtgtgtc gacactcatt aaaattagtc gctaatgaga
     6781 attagtcaaa ttaagcgcaa cgagaagata gagggaaaat atattttgag gaacattctg
     6841 gatatattaa caattacctg aggaataaat gacttagaga aaattattga ttttactggt
     6901 gttatgttgc gggattaatt tgtttgatta tattactgtt gggcggaaaa atgacgtaag
     6961 ttgacgttcg accggggtaa gcgaaacggt aaaaagataa atattaaatg aatttaggat
     7021 ttttccggct tcattaaaga aagttaaaat gccgccagcg gaactggcgg ctgtgggatt
     7081 aactgcgcgt cgccgctttc atcggttgta caaaaacttt cagtgccgcc agcattttct
     7141 ctggctcatt aatatgttgc tcgatgattt taacaatggc cgaaccagaa atcgcgcccg
     7201 cagctcctgc atcaatcgct gcttttacct gatccggggc ggaaatacca aacccctgca
     7261 atggaggtgc agcgttgtac tctttcagct tcgcaaccag atgattgagg ggtaacgcgg
     7321 cgcggttttc tgcgccggtc acgcccgctc gtgacagcaa atatgtgtaa ccacgaccgt
     7381 aagaggctat ctggcgcagc aggtcgtcat cggcatttgg cgggcagatg aagataggtg
     7441 cgacattatg gcgcaacgcg gcctggcgga agggcgcgga ctcttcaact ggcacatcgg
     7501 caaccagcac cgaatcgacg ccgactttct cgcactgggc ataaaactca tcaatgcctt
     7561 tgttaaacac cagattggca tacatcaaca ggccaatggg aatggtcggg tgtttctggc
     7621 gaatcagtgc cagcatttca aaacattgtg ccggagtcac acctgccgca aaggcgcgca
     7681 gagtggcgtt ttgaatggtc gggccatccg ccagtgggtc ggagaagggg atacctaact
     7741 ccagcgcgtc agcaccggct tcaattagcg tatcgataat tttcaatgac tgctcaatgc
     7801 ccggatcacc gagcgtgacg aaaggaacga atgcgccttc tttgcgctcc ttcaactggg
     7861 caaacagaga ttcgtagcgt tccatcagat ttcccctcgt gctttcaaaa tatcgtgaac
     7921 ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc accagtagct gctctttatc
     7981 cgggttttcg cgcatcattt tcagcgcatg ggccagggcg tgggaggatt ccagcgccgg
     8041 gatgatccct tcgtgcaggc acagcgtttt gaaggcttca agggcttcat catcggtaat
     8101 agacacgtaa tcagcgcgtc cagtgctgtt aagatacgcg tgttgtgggc cgacagacgg
     8161 gaaatccagt ccggcggaga tggagtaaga ttcttcaatc tgcccgtctt cggtttgcat
     8221 catcggcgct ttcataccga aataaatgcc cacgcgacca tgttttagcg gtgcgccgtg
     8281 ctcgccagtt tcgataccgt gaccaccagg ctccacacca atcaggccga cgttggtttc
     8341 attgatgaaa tcagcaaaca tgccgatggc attcgaaccg ccgccaacac aggcgataac
     8401 ggcatccggc aggcgacctt ctctttccag aatctgcgct ttggtttctt cgccaatcat
     8461 ccgctgaaac tcacggacaa tggtcggata aggatgcggg ccagccgcgg tgcccagcat
     8521 atagtgcgcg gtttcataac tgccggacca gtcgcgtagc gcctcattac aggcatcttt
     8581 cagcgtggcg gaaccgctat gtaccgggat cacttccgca cccattaagc gcatacgaaa
     8641 aacgttaggc gactggcgtt caacgtcttt ggcacccata taaatacggc atttcaggcc
     8701 gagcagggcg ctggcaaggg ccgacgccac gccatgctga ccggccccgg tttcggcgat
     8761 gatttcggtt ttacccatcc gcttcgccag caacgcctgc cccagcacct ggttggtttt
     8821 atgtgcgccg ccgtgcagca aatcttcacg cttgagatac agcgtggtgt tcgtcccggc
     8881 tgtaatgttc tggcatttgg tcagtgcggt tggacgcccg gcatagtttt tcagcaggtc
     8941 gttgaactgg gcttgaaatt caggatcttt ttgcgcgctg acaaaagctt cttccagctg
     9001 gcgcagagca ggcatcagga tttgtggcac gtacatgccg ccaaactcac caaaataggg
     9061 gttaagtaat gttgtcattg ttcctttcct taatatgcgc gcagcgtctg gaaaaccgag
     9121 gccaaaagac gtgcgtcttt gatgcccggt tgcgactcta cagcagaatt aaaatcaagt
     9181 ccggcgcagc cggtttgtgc cgcttccacg cagttatctg cgcctaagcc ccccgccagc
     9241 agaacgttgc caagcgattg accatttaat agtgaccagt caaaacgttg cccgcttcca
     9301 ccctggccgt tgtctaaaac atatttatca acgtgctgaa actcgcgggc gggcagggtt
     9361 tcaccgacgc ttaatgcttt ccagatggca acatgtgctg gcagagcttc acgcagcgta
     9421 tcgatataca gctgatcttc attaccatgc agttgcactg ccgccagcga taacacctta
     9481 gctttgtcca ccacatcggc aatatcgtga ttgcggaaca cgccaacata ctgcaacggt
     9541 gccgcagcca tcacttcctg cgcctgttca acgttgacgc aacgcggtga tgtcgcaaca
     9601 aaaatcaacc caccgtaaat cgcgcccgcg tcataagctg ctttagcatc ttgcccacgc
     9661 gtcaggccac atactttatt ctcacccagc aacacccggc gcacggcggc gtgcaaatcg
     9721 tcatgggcca tcaacgccga accaatcaga aaaccgttag cgaagtggct taactcgcgc
     9781 acctgagcgt aagtattgat gccggattcg ctgattaccg ttacgttgtg ccccagtttc
     9841 ggcgcaagct cgcgggtacg gttgagatca atcgacaaat cacgcagatc gcggttgttg
     9901 atgccaacga cctttgcccc taatgcaatg gcgcgctcca gttcctcttc attactgact
     9961 tcggtcagca cccccatctc cagactgtga gcgacggctg caagctggcg atattggtcg
    10021 tcatccagta ctgaaagcat taataagcag gcatcggcct ggtaatagcg cgccagatag
    10081 atctggtaag ggtcgataat gaagtcttta cataaaatcg gctgcggggc gatttggctg
    10141 acgatgagga ggaaatcaaa gctcccctga aaatatttct catcagtcag caccgaaatt
    10201 gccgaagcgt aatgtttata aatggcggca atgcgtgccg gatcgaaatc atcacggatc
    10261 acgccttttg acggcgacgc ttttttacac tccagaataa acgccgtgcg tgcaccctgt
    10321 agcgcatcat aaaaatgtcg tgtgctcggc tgaacctcat tctgaaaact ggccagcggt
    10381 tgctgctgtt tgcgggtttc tacccaaatc gccttgtctg cgacgatttt cgctaaaacg
    10441 gtttgcatca tttaccctcg tgcagccagc gcggtaactc tgtcgtaagc ggaaccactg
    10501 cgcagtacct caagaacggt ttgcgcattg gcttgcagat cttcatggcc atgcaggcgc
    10561 attaacatgg cgacgttcgc agcgacggct gcttcatggg cggcgtcgcc tttaccttgt
    10621 aacaagcgtg ttaaaatgtc acggttttct tcaggtgttc cgcctgccag ttgctcctgg
    10681 tggtagggag tcaggccaaa atcttcagcg gtcaattgat agctcttaat tttgccgtca
    10741 tgcagctcgg caacgattgt cggcgcgtgt aatgaaactt catccatccc gccgctgtgc
    10801 accaccgccg cgcgttgata ccccagcaca cgcaaggttt cggcaatcgg cagcaccagt
    10861 tccggactat aaacaccaat taacgccagc ggcggatgcg ccgggttaat caatggcccc
    10921 agcacattga acagggtgcg ggttttcagt tgctggcgaa ccggcatcgc gtggcggaat
    10981 ccggtgtgat atttcggcgc aaagaggaaa catacgccta actcgtccag cgcctggcgc
    11041 gatttattgg cgttcatatc aagattaata ccgaatgccg ccagcagatc cgacgagccg
    11101 gatttactgg agacgctgcg gttaccgtgt ttcgccactt tcagcccaca ggccgcggcg
    11161 acaaacgcac tggcggtaga aatattgata ctgttgctgc cgtcaccgcc agtaccgacg
    11221 atatcagcaa acagataatc cgggcgcggg aacggcgctg cgttttccag tagcgcggtt
    11281 gctgccccgg cgatctcgtt cgggtgctca ccgcgaattt tcatgctcac cagcgccgcc
    11341 gccagttgtt ccggcttcag ctcgccacgc accaccgctg aaaacagctg gtggctttct
    11401 tgttggctaa gcgtctgcgc ctgatacagt ttttccagaa tcggttgcag cgtgttggct
    11461 ggctctagtt tctgctgcgc ccaggccagc gtttgttcca gcaggcgagc gccctgggtg
    11521 gtgagaatgg attccggatg gaactggaat ccacaaacgc gatccgcatc gtgacgtact
    11581 gccatcacca tgccattaaa atgggcgttg atggttaaac cggccggaat gttactgcca
    11641 accagcgagt gataacgcgc caccggcagc gggtttgtta atccggcaaa catcgcctga
    11701 ccgtcatgtt caatgctcga cgctttaccg tgaagaattt cgcccgcctg accgacatag
    11761 cccccgtaag cttcgacaat tgcctgatgt ccgaggcaaa tgccaataat gggcagcttg
    11821 ccacgcaagc gggtgaggag ttccggcata caaccggctt cgctcggcac accggggcca
    11881 ggagaaagca tcagtaccgg attgctcatc gtcgccaggc gttcaattaa ggtctgcgcc
    11941 ggaatatggt tgcggtaaat caccacgtta tgaccattgc tgcgcaactg atctgccagg
    12001 ttgtacgtaa aagagtcgat attatcgagc agcagaatgt cagccatcag aaagtctcct
    12061 gtgcatgatg cgcggtggca atagcgcgca gtacagcgcg ggctttgtta cgggtttcgt
    12121 cggcttccga ctgcggaaca gaatcaagga ctacaccagc acccgcttgc acggtggcga
    12181 taccgttttc caccagcgcc gagcggatca caatgcaggt gtcgagatcg ccatgcgcgg
    12241 tgaaataacc taccgcgccg ccgtagctgc cgcggcgacg accttccgcc tcggcaatta
    12301 actgcatagc gcgtactttc ggcgcaccgc ttaacgtccc catattcata caggcgcgat
    12361 aagcgtgcag ggcgtcaaga tcgtgacgca gttcgccgac tacgcgagag acgaggtgca
    12421 tcacatagga ataacggtca actttggtga gatcggcgac gtagcggctg ccaggggtgc
    12481 aaatgcgtgc cagatcatta cgggcgagat caaccagcat cagatgttca gacagctctt
    12541 tatggtcagt acgcatttcc agttcaatac ggctgtcgag atctctgtcc agtgaaccat
    12601 cggcgcgacg accgcgtggg cgtgttccgg caatcgggta gatctcaatc tggcggctgg
    12661 tggcatcata cttgagcgag ctttccggcg acgcgccaaa tagggtgaaa tcattatcct
    12721 gcataaaaaa catgtacggg ctgggattac tctttttcag cacgtaatag gccgccagcg
    12781 gtgacgggca gggcagagag aaacggcgag atggcaccac ctggaaaatt tctccagcgc
    12841 gaatcgcttt ttgcaacaaa cgcactacgc caccgaactc ttcatcgctc tgattacatt
    12901 cacaacgcat atgcggcacg gaaaccactg gcagcggcgg cgcggcttcg gtcagttgct
    12961 gacgtagttc gttcaggcga gcagtaagac gttgtttttc ttcttcattc ggagcaaaca
    13021 ggctggcctg aatacgggtg ctttttttct gatggtcaat caccatcagc gtttcagcga
    13081 gataaaaaca gaaatcaggg cagttatttt ccgctgacag ttgcggtaaa tcttcaaatc
    13141 ccgccacaag gtcataagag aacaggccgc cgaagaacat tgcttctcgt tcttccttcg
    13201 gtacattcaa cagattctgc aataaacgga aagcgtcaaa aaccgaaagg gagcataagc
    13261 gggcgtcttc atccagcagt ggactgacag gggggaagcg cagcacacgg cagtttggtg
    13321 attgttcact ttccacaccc gcaggcaggg cgttatccag tagtgccagc agggcttcgc
    13381 cattgccgga aagtgcctga attgtgacgg tgtcacctaa tgctgtaatg tgcagcgcac
    13441 tgtctatcag cagcaggctt tttaaatcat ctttgctgtc gatatctgcg gattccagca
    13501 gcagcgttgc cggacgattc ccacacaact ggtgaaaaag cgcagtcggg ttgtcgcgat
    13561 aagcgccttc gcaggttagc agttcgagag tcggtttttg tgtttgcatt gttattctct
    13621 aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg gtatctgatt gctttacgca
    13681 tggtgaatac actgcccgtt tcaggaagtg gccaccaacc tttcagtacg aaaattgctt
    13741 tcattgtcga taccattttt acgtgaactt gcgtactagt caactggttc gatgattaat
    13801 tgtcaacagc tcatttcaga atatttgcca gacccgttat gatgtcggcg caaaaaacat
    13861 tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta cgacctgcac
    13921 agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca ccgtgcagtc
    13981 gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca ccacagctgc aatcgcgcca
    14041 gcaagagagg aaatttcacg ttcgggactg gcgctgaatc ttattcccgg cgtggaaatt
    14101 tccacggtct gggaaaatca tgaaattcat attgtcgggc tgaatattga tattactcat
    14161 ccgctaatgt gtgagttcct cgcgcagcag acagaacggc gcaatcagcg ggcgcagcaa
    14221 attgccgaaa gacttgaaaa agcgcaaata cctggggcgc tggaaggcgc acaacgactg
    14281 gcgcagggcg gcgcagtgac gcgcggtcat tttgcgcgtt tccttgtgga gtgcggcaaa
    14341 gccagttcaa tggcagatgt ctttaaaaag tatctggcgc gcgggaaaac cggatacgtt
    14401 ccgccacagt ggtgtacaat agaacaagct attgatgtca ttcatcattc tggcggtaag
    14461 gcggtgctag ctcatcctgg gcggtacaat cttagtgcta aatggctgaa aagattggtt
    14521 gcgcattttg ccgaacacca cggcgacgcg atggaagtcg cgcagtgcca gcaatcgccc
    14581 aatgaacgca cccagctggc cacacttgcg cgtcagcatc atttatgggc atcacaagga
    14641 tctgattttc atcagccatg cccgtggatc gaactgggtc gtaaactctg gctgcccgca
    14701 ggcgttgaag gcgtctggca gctatgggaa cagccgcaga acaccacaga gagggaatta
    14761 tgagccagtt tttttatatt catcctgata acccgcagca acgtctgatc aaccaggcgg
    14821 tggagatcgt gcgtaaaggc ggggtgattg tctatccaac tgattccggc tatgcgctcg
    14881 gctgtaaaag tgaagataaa aacgccatgg agcgtatttg tcgtattcgc cagctgccgg
    14941 acggtcacaa ctttaccctg atgtgtcgcg atctttctga actctcgacc tattcattcg
    15001 tggataacgt tgcgtttcgt ttgatgaaaa acaacacgcc gggcaactat accttcatcc
    15061 tgaaggggac gaaggaagtg ccacgccgtc tgttgcagga gaaacgcaaa accatcggta
    15121 tgcgtgtgcc ttctaacccg attgcgcagg cgttgcttga agcactgggc gaaccgatgc
    15181 tttccacttc gctaatgctg ccaggcagcg aatttaccga atccgatccc gaagaaatta
    15241 aagaccgtct ggaaaaacag gtagatttga ttattcacgg cggctatctc gggcagaaac
    15301 caacaacggt tattgatctt accgatgata cgccggtcgt ggtgcgtgaa ggcgtaggtg
    15361 atgtgaagcc tttcttataa gtgcgtaact ctgtatacta cgcggcctta aaatggcgct
    15421 atacggcgcc attgattctc ctgttcgacg cctgtgaagg cgacaccaaa aggaagctct
    15481 atgagcgaaa aattacagaa agtgctggcc cgcgctggcc acggctcccg ccgtgaaatc
    15541 gaatctatta ttgaagccgg tcgtgtgagt gttgatggca aaattgctaa actcggcgat
    15601 cgcgttgaag ttacccctgg gctgaaaatt cgtatcgatg gtcatctgat ttcggtacgt
    15661 gagtccgctg aacaaatttg tcgcgttctg gcctattaca aaccggaagg tgagttgtgt
    15721 acccgtaacg atccggaagg gcgtccgacg gtgtttgacc gtctgccaaa gctgcgtggc
    15781 gcacgctgga ttgccgtagg tcgtctggac gttaacacct gtggtctgct gctgttcacc
    15841 accgatggtg aactggcaaa ccgtttaatg catccaagcc gtgaagttga acgtgaatac
    15901 gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc gtgatttgag tcgtggcgtg
    15961 caactggaag atggtccggc agcttttaaa accatcaagt tcagcggcgg cgaagggatc
    16021 aaccagtggt acaacgtgac tctgaccgaa ggccgtaacc gtgaagttcg tcgtctgtgg
    16081 gaagcggttg gtgtgcaggt gagccgcctg atccgtgttc gttacggtga tatcccactg
    16141 ccaaaaggtc tgccacgcgg tggctggacg gagctggacc tcgcccagac taactatctg
    16201 cgcgaactgg tggagcttcc gccagaaacc agctctaaag tcgctgtaga aaaagaccgt
    16261 cgtcgcatga aggctaatca gattcgtcgt gcggtgaaac gtcacagtca ggtgagcggc
    16321 ggtcgtcgtt ctggcgggcg taataacggt taatcaaagt atgccggacg tcatatccgg
    16381 catttttaca gattaataat caatccctat ttgcgcctta acaccggcat caaacgcatg
    16441 tctgatgggg cgcagctcac ttaccgtatc cgccagttca agaatatccc gatgacagcc
    16501 tcgacccgtg ataatcaccg tctgttgatg tggacgttca tttaacgcct gcaccacttc
    16561 ttccagtggc aaatagtcat acgccaccat atacgtcagt tcatcaagca aaaccatatc
    16621 cagtgaggaa tcagcaagca tccgctttgc atgttgccag acttcgcggc aggcggcggt
    16681 atcagactcg cggttttgtg tatcccaggt aaagcccgtt gccatcacct gaaactcaac
    16741 gccatgtggc tccagcagat tgcgttcgcc attaggccag gtgcctttaa taaactgcac
    16801 gacgcccact ttttttccgt gaccaactgc gcgtgttgcc gtaccaaatg ccgcggtggt
    16861 tttgcctttt ccattgccgg taaagacgat gataataccg cgttcatcct gggcctgggc
    16921 cacacgagca tctacttttt ctttcactcg ctgctgacgc tgttggtagc gttcatcact
    16981 cattgggaaa ttcctggttt acggcccggt tgggcgtcaa aggtcatgcc ggttttacgg
    17041 cggctgtcat cgcccatcag ccagaggtag agcggcatga tatcagcggg tgttttaagt
    17101 ttctgtggat cttcggtcgg gaaggcgctt gcgcgcattg cggtgcgcgt accgcctggg
    17161 ttaatgcagt tgacgcgcag gcgctgctga tattcatcgg ccagtacctg catcatccct
    17221 tcggtggcaa atttcgacgc tgcatatgca ccccagttgg ctcgtccctg acgtccaacg
    17281 cttgatgaag taaagaccag tgaaccggcg tccgatttga gtaataaagg aagcagtgcc
    17341 tgggtgagca taaaggtggc attaacgttg acctgcatga cgtcctgcca gacctgcgga
    17401 ttttgttcgc tcattgggca aacatcgccg agcaatccgg cattatgcaa aacaccatcc
    17461 agacgcggat aattaacgac aatgcgctgt gccagttgtt ggcaattttc ggacgtgcag
    17521 gtcagcaaat cgagaataaa ccactgtggc tgacgcccag tttcttcgtt tatgtggctg
    17581 gctacctgac gtaatttttc ttcattacgg cccaacagaa tcactgtcgc accatagcgt
    17641 gcatacgtca tcgcggcttc acgaccaatc ccatcgctgg ctcccgtcac caggataatg
    17701 cgatcattga gtaaatcttg ttttggctgg taatgcatgg ctactcctca acgacgttgt
    17761 ctgtcgcgct gccagtgtac tttatgactt tggggcttta tgcctgaaac agaagggcat
    17821 ttcaatcagc ctggggcgat aacaccgcaa aatggatact ttgtcatact ttcgctgcaa
    17881 taacatctct gcgagacggc ttaacatgcc tgttgtaaac tgtgagccaa agcgttgttt
    17941 aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg tttgtttttg gctaaaatcg
    18001 ttaccgttgt gctagcgatt gcggcgattg ccgccattat tgtcaatgtt gctcaacgta
    18061 ataaacgcca gcgtggcgag ttacgggtca acaatctcag cgaacagtat aaggagatga
    18121 aagaagaact ggccgcggcg ctgatggact cacatcagca aaaacagtgg cacaaagcgc
    18181 agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc gaaagccaaa ctgggcgagg
    18241 tggcaactga cagtaaacct cgcgcctggg tgctggattt taaaggcagc atggacgccc
    18301 atgaagtgaa ctcgctacgt gaagagataa cggcggtact cgcagcattc aaaccgcagg
    18361 atcaggttgt gctccgtctg gaaagccctg gtggcatggt gcatggttac gggttggcgg
    18421 cttcgcagct gcagcgtctg cgtgataaaa acattccttt aactgttacg gtagacaaag
    18481 tcgctgccag cggcggttac atgatggcct gtgtggcgga caaaattgtt tccgcaccgt
    18541 ttgctattgt gggttccatt ggggtggtgg cgcaaatgcc caactttaac cgcttcctga
    18601 aaagcaaaga tattgatatc gaactgcaca ccgccgggca gtataagcgc acgctgacct
    18661 tgctgggtga aaataccgaa gaagggcggg agaaattccg cgaagagctg aacgaaacgc
    18721 atcagttatt taaagatttt gtgaagcgta tgcgtccgtc tctggatatt gaacaggtgg
    18781 caacgggtga acactggtac ggacaacagg cggtagagaa aggcctggtt gatgaaatca
    18841 acaccagtga tgaagttatt cttagcctga tggaaggccg tgaagtggtt aatgtacgct
    18901 atatgcagcg taaacgactc attgaccgat tcaccggcag cgcggcagag agcgccgatc
    18961 gattgttgct acgctggtgg cagcggggtc aaaagccatt gatgtaaaag acaaacgcga
    19021 ggctaagacc tcgcgttttg ctttaatcaa ccagatgata tttttctgaa agcacatggg
    19081 ccaggtgttt gaacatatta aacaccgcgg tgcttttggc tgttggcaat ccttgttcat
    19141 ctaaaaagta gtcgccggta aataccagca cgccattacg ctgcgtgacg ccggtggctt
    19201 caatccctgc gagcgtatcc tcatgctcac gaatgatttt gttagcctct ttcaacagcg
    19261 tttcgcggtc gatgggttgt gtctctttgt tcattattta ctccttaaac aaggacatta
    19321 gtctacgcca ggcatggctt gcagacaaat ataccacgct ggtggcaaga gcgccttact
    19381 ggcaactttg gattttgcat gctaataaag ttgcgtatcg gattttatca ggtacagtgt
    19441 gacgctttcg tcaatctggc aatagatttg cttgacattc gaccaaaatt ccgtcgtgct
    19501 atagcgcctg taggccaaga cctgttaact cagtcacctg aattttcgtg aacagagtca
    19561 cgacaagggg gtgatatccg cagagagcga gtccatatcg gtaactcgtt gccagtggaa
    19621 ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt aggtaaggtg aatatgggta
    19681 aagctcttgt catcgttgag tccccggcaa aagccaaaac gatcaacaag tatctgggta
    19741 gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga tttgccgacc agtggctcag
    19801 ctgccaaaaa gagtgccgac tctacctcca ccaagacggc taaaaagcct aaaaaggatg
    19861 aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg gcacaattgg gaggcgcatt
    19921 atgaagtgtt gcctggtaaa gagaaggtcg tctctgaact gaaacaactg gctgaaaaag
    19981 ccgaccacat ctatctcgca accgaccttg accgcgaagg ggaagccatt gcatggcacc
    20041 tgcgggaagt gattggtggt gatgatgcgc gctatagccg agtggtgttt aacgaaatta
    20101 ctaaaaacgc gatccgccag gcatttaaca aaccgggtga gctgaatatt gatcgtgtta
    20161 atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg gtatatggtt tcgccgctgc
    20221 tgtggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt gcagtcggtg gcggtccgcc
    20281 tggtggtaga gcgtgagcgt gaaattaaag cgttcgtacc ggaagagttc tgggaagtcg
    20341 atgccagcac gactacgcca tctggtgaag cgttggcgtt gcaggtgact catcagaatg
    20401 acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc tgcggtaagt ctgctggaaa
    20461 aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac aaccagtaaa cctggcgctc
    20521 cttttattac ctctacgctg caacaagctg ccagcacccg tcttggtttt ggcgtgaaaa
    20581 aaaccatgat gatggcgcag cgtttgtatg aagcaggcta tatcacttac atgcgtaccg
    20641 actccactaa cctgagtcag gacgcggtaa atatggttcg cggttatatc agcgataatt
    20701 ttggtaagaa atatctgccg gaaagtccga atcagtacgc cagcaaagaa aactcacagg
    20761 aagcgcacga agcgattcgt ccttctgacg tcaatgtgat ggcggaatcg ctgaaggata
    20821 tggaagcaga tgcgcagaaa ctgtaccagc taatctggcg tcagttcgtt gcctgccaga
    20881 tgaccccagc gaaatatgac tccacgacgc tgaccgttgg tgcgggcgat ttccgcctga
    20941 aagcacgcgg tcgtattttg cgctttgatg gctggacaaa agtgatgcct gcactgcgta
    21001 aaggcgatga agatcgtatc ttaccggcag tcgataaagg cgatgctctg acgctcgttg
    21061 aactaacacc agcccagcac tttaccaagc cgccagcccg tttcagtgaa gcatcgctgg
    21121 ttaaagaact ggaaaaacgt ggtatcggtc gtccgtctac ctatgcgtcg atcatttcga
    21181 ccattcagga tcgtggctat gtgcgagtag aaaatcgtcg tttctatgcg gaaaaaatgg
    21241 gcgaaatcgt catcgatcgc cttgaagaaa atttccgcga gttaatgaac tacgacttta
    21301 ccgcgcagat ggaaaacagc ctcgaccagg tggcaaatca cgaagcagag tggaaagctg
    21361 tactggatca cttcttctcg gatttcaccc agcagttaga taaagctgaa aaagatccgg
    21421 aagagggtgg tatgcgcccg aaccagatgg ttctgaccag cattgactgc ccgacttgtg
    21481 gtcgcaaaat ggggattcgc acagcgagca ccggggtatt ccttggctgt tctggctatg
    21541 cgctgccgcc gaaagagcgt tgcaaaacca ccattaacct ggtgccggaa aacgaagtgc
    21601 tgaacgtgct ggaaggcgaa gatgctgaaa ccaacgcgct gcgcgcaaaa cgtcgttgcc
    21661 cgaaatgcgg cacggcgatg gacagctatc tcatcgatcc gaaacgtaag ttgcatgtct
    21721 gtggtaataa cccaacctgc gacggttacg agatcgaaga gggcgaattc cgcattaaag
    21781 gttatgacgg cccgatcgtt gagtgtgaaa aatgtggctc tgaaatgcac ctgaaaatgg
    21841 ggcgtttcgg taaatacatg gcctgcacca acgaagagtg taaaaacaca cgtaagattt
    21901 tacgtaacgg cgaagtggca ccaccgaaag aagatccggt gccattacct gagctgccgt
    21961 gcgaaaaatc agatgcttat ttcgtgctgc gtgacggtgc tgccggtgtg ttcctggctg
    22021 ccaacacttt cccgaaatcg cgtgaaacgc gtgcgccact ggtggaagag ctttatcgct
    22081 tccgcgaccg tctgccggaa aaactgcgtt atctggccga tgcgccacag caggatccgg
    22141 aaggtaataa gaccatggtt cgctttagcc gtaaaaccaa acagcaatat gtctcttcgg
    22201 aaaaagacgg aaaggcgact ggctggtcag cattttatgt tgatggcaaa tgggttgaag
    22261 gaaaaaaata acctttaatt ctgtcaggtt tttataaaca aagggtcgcg aaagcggccc
    22321 ttttttattg catattattt tttcttcaca cctatacact aaggctataa atgatatagt
    22381 ggttatagtt agcacctttt ttattattaa atcgtattag tcacccgcca ggtgtgacga
    22441 aaaaacgatg ttctgatggc gtctaagtgg atggtttaac atgaaattac aacaacttcg
    22501 ctatattgtt gaggtggtca atcataacct gaatgtctca tcaacagcgg aaggacttta
    22561 cacatcacaa cccgggatca gtaaacaagt cagaatgctg gaagacgagc taggcattca
    22621 aattttttcc cgaagcggca agcacctgac gcaggtaacg ccagcaggac aagaaataat
    22681 tcgtatcgct cgcgaagtcc tgtcgaaagt cgatgccata aaatcggttg ccggtgagca
    22741 cacctggccg gataaaggct cgctgtatat cgccaccacg catacccagg cacgctacgc
    22801 gttaccaaac gttatcaaag gctttattga gcgttatccc cgcgtttctt tgcatatgca
    22861 ccagggcccg ccgacacaaa ttgctgatgc cgtctctaaa ggcaatgccg atttcgctat
    22921 cgccacagaa gcgctgcatc tgtatgaaga tttagtgatg ttaccgtgct accactggaa
    22981 tcgggctatt gtagtcactc cggatcaccc gctggcaggc aaaaaagcca ttaccattga
    23041 agaactggcg caatatccgt tggtgacata taccttcggc tttaccggac gttcagaact
    23101 ggatactgcc tttaaccgcg cagggttaac gccgcgtatc gttttcacgg caacggatgc
    23161 tgacgtcatt aaaacttacg tccggttagg gctgggggta ggggtcattg ccagcatggc
    23221 ggtggatccg gtcgccgatc ccgaccttgt gcgcgttgat gctcacgata tcttcagcca
    23281 cagtacaacc aaaattggtt ttcgccgtag tacttttttg cgcagttata tgtatgattt
    23341 cattcagcgt tttgcaccgc atttaacgcg tgatgtcgtt gatgcggctg tcgcattgcg
    23401 ctccaatgaa gaaattgagg tcatgtttaa agatataaaa ctgccggaaa aataatttct
    23461 tgcgttattt tcggcacctt ttatgtagcg aaggtgccgg aatatattct cttttgttac
    23521 ttatttctat accgtgcaca atatttttat ccctcatatt tatagggtaa attacctgta
    23581 aagtagtgtc taattacccg cctcgcaaat tccagttttc aaatatctat ttcaggtcaa
    23641 aagattgaat tattcactgg agacgattcg taaattcgct ggatttttga ctaaagtttc
    23701 tttaggattt gtctcatcgt atgattaatt acaccaatca ttcaacgtct tgctgataag
    23761 tgatggcgat tatatgaggt tagcaatgcc ttctggaaat caggaacccc gccgtgatcc
    23821 tgaattaaaa cgtaaagcct ggctggcggt ttttcttggt tctgcacttt tctgggtggt
    23881 tgtcgcactg ctgatttgga aagtgtgggg ataactatgg tcggtcagga gcaactggag
    23941 tcgtcaccat tatgccagca tagtgacaat gaaccagaaa cgaaacggga atgttccgtc
    24001 gttattccag acgactggca actaacatcg cagcagcaag cctttataga actgtttgct
    24061 gaagatgatc agccgaaaca ataattatca tcattcttat tacccatttt taatgaatta
    24121 aagggctttt aatacaccgc agcaataaca gcttgagtta tctcaacaca aaataataac
    24181 cgttaagggt gtagcctatg atcaacacaa atatgaaata ttggtcctgg atgggcgcgt
    24241 tttctctgtc gatgctcttc tgggccgaac tcctctggat cattactcac tgatccttga
    24301 ccccgctgcg gcggggttgt catttgcttt gccacaaggt ttctcctctt ttatcaattt
    24361 gggttgttat caaatcgtta cgcgatgttt gtgttatctt taatattcac cctgaagaga
    24421 atcagggctt cgcaaccctg tcattaagga ggagctatgt cgtcaaccct acgagaagcc
    24481 agtaaggaca cgttgcaggc caaagataaa acttaccact actacagcct gccgcttgct
    24541 gctaaatcac tgggcgatat cacccgtcta cccaagtcac tcaaagtttt gctcgaaaac
    24601 ctgctgcgct ggcaggatgg taactcagtt accgaagagg atatccacgc actggcggga
    24661 tggctgaaaa atgcccatgc tgaccgtgaa attgcctacc gcccggcaag ggtgctgatg
    24721 caggacttta ccggcgtacc tgccgttgtt gatctggcgg caatgcgcga agcggttaaa
    24781 cgcctcggtg gcgatactgc aaaggttaac ccgctctcac cggtcgacct ggtcattgac
    24841 cactcggtga ccgtcgatcg ttttggtgat gatgaggcat ttgaagaaaa cgtacgcctg
    24901 gaaatggaac gcaaccacga acgttatgtg ttcctgaaat ggggaaagca agcgttcagt
    24961 cggtttagcg tcgtgccgcc aggcacaggc atttgccatc aggttaacct cgaatatctc
    25021 ggcaaagcag tgtggagcga attgcaggac ggtgaatgga ttgcttatcc ggatacactc
    25081 gttggtactg actcgcacac
//
LOCUS       DADLXX010000048        24861 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLXX010000048 DADLXX010000000
VERSION     DADLXX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.048"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..352
                     /locus_tag="JNX27_001958"
     CDS             71..352
                     /locus_tag="JNX27_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7883185.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1394
                     /locus_tag="JNX27_001959"
     CDS             318..1394
                     /locus_tag="JNX27_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7883186.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMADWIERYKTILIRRKASRNTYKTRVN
                     QLVTIKENLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGILFSENPPTFHEIRSLAGRLYKEICGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1787..2128)
                     /locus_tag="JNX27_001960"
     CDS             complement(1787..2128)
                     /locus_tag="JNX27_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7883187.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2141..3013)
                     /gene="copD"
                     /locus_tag="JNX27_001961"
                     /pseudo
     CDS             complement(2141..3013)
                     /gene="copD"
                     /locus_tag="JNX27_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="JNX27_001962"
     CDS             complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="JNX27_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7883188.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3530..3760
                     /locus_tag="JNX27_001963"
     CDS             3530..3760
                     /locus_tag="JNX27_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7883189.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3862..4518
                     /locus_tag="JNX27_001964"
     CDS             3862..4518
                     /locus_tag="JNX27_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY7883190.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4542..5204
                     /gene="exoX"
                     /locus_tag="JNX27_001965"
     CDS             4542..5204
                     /gene="exoX"
                     /locus_tag="JNX27_001965"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7883191.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="JNX27_001966"
     CDS             complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="JNX27_001966"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY7883192.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQHWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7470..8129)
                     /locus_tag="JNX27_001967"
     CDS             complement(7470..8129)
                     /locus_tag="JNX27_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7883193.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8456..8812)
                     /gene="yebF"
                     /locus_tag="JNX27_001968"
     CDS             complement(8456..8812)
                     /gene="yebF"
                     /locus_tag="JNX27_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7883194.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8879..9169)
                     /gene="yebG"
                     /locus_tag="JNX27_001969"
                     /pseudo
     CDS             complement(8879..9169)
                     /gene="yebG"
                     /locus_tag="JNX27_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9303..10481
                     /gene="purT"
                     /locus_tag="JNX27_001970"
     CDS             9303..10481
                     /gene="purT"
                     /locus_tag="JNX27_001970"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7883195.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10536..11177)
                     /locus_tag="JNX27_001971"
     CDS             complement(10536..11177)
                     /locus_tag="JNX27_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7883196.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGVGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11214..13025)
                     /gene="edd"
                     /locus_tag="JNX27_001972"
     CDS             complement(11214..13025)
                     /gene="edd"
                     /locus_tag="JNX27_001972"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7883197.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="JNX27_001973"
     CDS             complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="JNX27_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7883198.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15074..15943
                     /locus_tag="JNX27_001974"
     CDS             15074..15943
                     /locus_tag="JNX27_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7883199.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16071..17513
                     /gene="pyk"
                     /locus_tag="JNX27_001975"
     CDS             16071..17513
                     /gene="pyk"
                     /locus_tag="JNX27_001975"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY7883200.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17644..18615)
                     /gene="lpxM"
                     /locus_tag="JNX27_001976"
                     /gene_synonym="msbB"
     CDS             complement(17644..18615)
                     /gene="lpxM"
                     /locus_tag="JNX27_001976"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7883201.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18735..20057)
                     /gene="mepM"
                     /locus_tag="JNX27_001977"
     CDS             complement(18735..20057)
                     /gene="mepM"
                     /locus_tag="JNX27_001977"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7883202.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20073..21005)
                     /gene="znuA"
                     /locus_tag="JNX27_001978"
     CDS             complement(20073..21005)
                     /gene="znuA"
                     /locus_tag="JNX27_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7883203.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21084..21839
                     /gene="znuC"
                     /locus_tag="JNX27_001979"
     CDS             21084..21839
                     /gene="znuC"
                     /locus_tag="JNX27_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7883204.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21836..22621
                     /gene="znuB"
                     /locus_tag="JNX27_001980"
     CDS             21836..22621
                     /gene="znuB"
                     /locus_tag="JNX27_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7883205.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22768..23778)
                     /gene="ruvB"
                     /locus_tag="JNX27_001981"
     CDS             complement(22768..23778)
                     /gene="ruvB"
                     /locus_tag="JNX27_001981"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7883206.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23787..24398)
                     /gene="ruvA"
                     /locus_tag="JNX27_001982"
     CDS             complement(23787..24398)
                     /gene="ruvA"
                     /locus_tag="JNX27_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7883207.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24537..24602)
                     /locus_tag="JNX27_001983"
     CDS             complement(24537..24602)
                     /locus_tag="JNX27_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883208.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24673..>24861
                     /locus_tag="JNX27_001984"
     CDS             24673..>24861
                     /locus_tag="JNX27_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883209.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITT"
BASE COUNT         6165 a         6295 c         6108 g         6293 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcaga ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg tgacaataaa agaaaattta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtgg attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcctgt tcagtgaaaa cccaccaact tttcacgaaa
     1261 tcagaagcct ggctggacga ttgtacaaag aaatatgtgg agaagaattt gctcagcgtc
     1321 tacttggcca cacatcggag aagacaacaa aaatgtatct tgatgagaga gaaaaaacgt
     1381 acttactgct ctgattttaa cgtaaatgga ttgttaaatg tattttggtt gtgatataac
     1441 caaaaaagac cggaatacag aaattcgagt aaatttcggg gaatttcggg gagacgtttg
     1501 caactaattg attttaaata caattaaaaa aagaccgaat acgattcctg tattcggtcc
     1561 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
     1621 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
     1681 caataaaaag cgcggtggtc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga
     1741 actgaggcgt tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
     1801 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
     1861 tctgaatcac cgaaatgggt tgggcattgg tcttcccact ggcaacttcc ttttgtgcga
     1921 tatcgtttaa aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg
     1981 gtcggcaagt cagcattacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
     2041 aacgactcac ggttatcacc tgtggagccg ccagcgccgc tgcagaactg gtgagtaaca
     2101 gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
     2161 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
     2221 acacaaacca gggtgccgca cgatcttcat cctgcctcat gcgcggtaca agaacataac
     2281 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
     2341 ggccctagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
     2401 cgcttgccag tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct
     2461 gaatagcctg gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
     2521 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
     2581 tcgctgttac cccttcattc agcgtcgcat gccccactcc cgccagcaga ataaattgcg
     2641 cggtagtgag cataaacaac aaacgtggca tattccgcgg ttgcataagg gcgacgatca
     2701 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
     2761 cagcctgcca aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct
     2821 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
     2881 gcagagaacg cttcgtcagc aagcgacgaa tcattaaggg ggccagccag gcaccataca
     2941 ttgcaaagcc aaagaccagc atcaacgagg tgaaatggat aaatcgcagc gcgatccagg
     3001 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
     3061 ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg
     3121 gaacaatcag ttgcttttgg tcctgctcat tccgcttcgc tggtaatgtt ttaatatttt
     3181 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttcaacgcct tccgagaagt
     3241 ttaaagtgat tgcctgcggg gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
     3301 ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgtcagcg
     3361 cgtagcgaag ggagcgtgca gttgaagcca tattatctat tcctttttgt aataactttt
     3421 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
     3481 caggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
     3541 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
     3601 ggtggctttt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
     3661 gccagaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
     3721 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact
     3781 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcccccat aatttgcagt
     3841 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
     3901 cgtaaaacct cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc
     3961 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
     4021 cgacgtgccc ttcctgcccc acccgatctt tcactgttag acccgctttg ttatgccgca
     4081 acgacctggc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
     4141 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggaactacca tcagagtcat
     4201 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt
     4261 atggatatgg acccgacctg ttcactgttt acgacagggc aatgtctggg agaacccgac
     4321 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg
     4381 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
     4441 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
     4501 atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata
     4561 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
     4621 gaaaaatcgt caaccccatg agcctcctgg tgcgccccga tcgtcctatt agtccacaag
     4681 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
     4741 tgatcccaca ctattacggt agtgaatggt atgtcgcgca caacgccagc tttgaccgcc
     4801 gcgtactgcc tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt
     4861 ggccagggct caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga
     4921 caccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
     4981 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
     5041 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
     5101 ttgccgaacg cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
     5161 cggagctgcg tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagtgg
     5221 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
     5281 atttaaagcg gccagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
     5341 gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatgtt
     5401 gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
     5461 cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcggatttc
     5521 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
     5581 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
     5641 caacgcccat caacatgccc cccgcacttc cgcccatcgc ataacagagc gaaggagagc
     5701 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
     5761 tcttcagaaa tttaccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat
     5821 ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg
     5881 catcaatact tgcgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc
     5941 gaaaatgttt gcgatgatag accagcgaaa ccggaacttc gacgccatca cgggcgacta
     6001 tccacaggtg ttcactgcgg taattcgccg catcaaaacc gggcacttct gtttgtttta
     6061 atacacgacg ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg
     6121 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
     6181 cataggccgg atcatcaaag gcaatgccaa tgacttcccg ggtcttgcgg ttaatttggc
     6241 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
     6301 acccttccag catgatgttt tcacgtggcg gaattaactc ttcccactgt tgctcatcac
     6361 gcatacgggt acggtataag ccaaagtttt tgccgtggcg attggaacgc agataaaaac
     6421 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
     6481 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
     6541 gatgaatgac gacatagtgc ttcgacgtcg ttttatgcag gctgacataa taggtatcgt
     6601 ctttttcttc gtagatcagt gtatcttgcg atgctggcgt accgatggcg tgacgccaga
     6661 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
     6721 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttacctgttt
     6781 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
     6841 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
     6901 cgcgcttgtt ggcatcgagc aatgtttccc actcatccca ctcttcgctg aatgccgatt
     6961 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
     7021 tttttatgta gggcgcagaa acttctcttt gcggaatgcg gtcgatgatt tcctttaaga
     7081 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
     7141 gcaggtagtc caggacttct ggctgagaac gcgtatcgtc ccgcagccag tagtaattat
     7201 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
     7261 tgttattgtt ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca
     7321 agcgaaacag ggtcgtcatt gcttagatga tgacaggtaa tggcgcggat atcgaatgtt
     7381 atgcagacag agaaaatcag cctgttcaaa tggctgtgcg gttctggata gcccgaaata
     7441 gtcaacttca ggctatccag agagcggaat tattccgcca aagtgcgttt ttgctgttcg
     7501 agatcgcgtt caatgccttc acgaacatcc tgggggattt tcagcgcgtc acccagtgca
     7561 ttcaggtaac tgcgttccat aaaatggtca atatcaatag ccgcgcagct caggaaatag
     7621 atttccagcg cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt
     7681 tgttcgattg cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat
     7741 tgctggtcga ttgccgcacg ttcattggca tcaatatgac catcactttt agcggcaaag
     7801 accagcgcaa ggatcaaacg ttccgtacgc tcatccagcg gcgtactttg cgcgccaaac
     7861 tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta
     7921 cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta
     7981 cgtgctgatt tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac
     8041 agtttgccca atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg
     8101 gattgcagtt gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct
     8161 gtagcatagc ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag
     8221 cggggcggta ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca
     8281 ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct
     8341 gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctggga
     8401 cggacaaggc gctcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac
     8461 gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg
     8521 cacttttacc acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct
     8581 gcaaactgac ccaggccacg ggatcggcct gaccgacaat tttttgatca tctgcccagc
     8641 gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg gtagcatcca
     8701 gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg
     8761 atgcgcaggc agaaaccaac aaccgcccta aaaacgcccc tctttttttc atgtttttct
     8821 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
     8881 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgaccagt ggggacggta
     8941 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
     9001 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagctag gtgtcaagca
     9061 gatccgccgt atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca
     9121 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
     9181 cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa
     9241 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
     9301 ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct
     9361 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
     9421 ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
     9481 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
     9541 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
     9601 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
     9661 tggcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat agcaaaagcc
     9721 ttttccgcga ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct
     9781 cttccggcaa ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat
     9841 acgctcagca aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt
     9901 ttgacttcga aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
     9961 cagtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga
    10021 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
    10081 gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg
    10141 tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt
    10201 ttgccctgca tgtacgtgcc ttcctcggac ttcctgttgg cgggatccgt cagtatggtc
    10261 ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg
    10321 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
    10381 gcagccgtcg tctgggggtg gtactggcta cttcagagag tgttgttgac gccattgaac
    10441 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaacgctac
    10501 aaaaatgccc gatcctagat cgggcatttt gacttttaca gcttagcgcc ttctacagct
    10561 tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc
    10621 agccaggaac caccgatgca cagcacgctt ttcagtgcca ggtagtcacg gtagttagcc
    10681 ggagaaatac cacccgtcgg gcagaaacgg acctgggaga acggacccgc gatcgcctgc
    10741 agggctttca cgccgccgtt agcttcagcc gggaagaatt tgaactcttt caaaccgtag
    10801 tccataccca gcatcagttc ggaaacagtg ctgatccccg gaatcagagg aatagtccct
    10861 tcggtagcag ctttcagcag cggctcggtc agacccgggc taattgcgaa ctgtgcaccc
    10921 gcttcagtga cttctgccag ctgctgtgga ttcagcaccg taccgacacc cacaatcgct
    10981 tcaggcactt ctttggcgat agcacggata gcgtcaactg cacactcggt acgcagagtc
    11041 acttccagaa cgcgcacccc accagcaacc aacgcttttg ccatcggcac cgcgtgttcc
    11101 agttttttta ccacgataac cggtacaacc gggccggtgg tcaggattga ttctgcactt
    11161 gttttccagt ttttcatcag agttttctct cgcctgatta caaatttgtc gtcttaaaaa
    11221 gtgatacagg ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc gctgaataat
    11281 tcacgtcctg ttctcacgcg tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc
    11341 agttccgctt cgtctaccag cagtgtcagt tcgcctgtct gtccattcac acgaatgatg
    11401 tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg cttctggtgt tacgtggata
    11461 gctgacggca ctttacctga agcgccggag agtcgtccat cggtaactaa cgcaattttg
    11521 aaacaccggt ccaataatac accaagtggc ggcatgagtt tatgtaattc tggcattccg
    11581 ttcgcttttg gcccctgatg acggacaaca acgacacaat cgcggtccag caaacccgct
    11641 tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc
    11701 tggttctcaa ccggcacggc agaggttttc ataaccgcac ggcccaggtt accgcttaac
    11761 acttttgtcc caccatgatg agagaaaggt tgttcgaagg aagcgatcac attgctgtcg
    11821 agtgattttt ccgccccttc ccgccagtcc agttcaccat tattcagcca tggttcaagg
    11881 gtataacgaa acagaccaaa gcctgccacc gtattgacat cttcatgcag caggcctgct
    11941 ttgagcagtt cacgcaccag aaccggtacg ccacctgccg cctggaagtg gttaatatcg
    12001 gccggaccgt tcgggtagag acgtgccatc agcggtacaa catcagaaag gtcagagaag
    12061 tcatcccagt taatctgaat accggccgcg cgcgccatcg ccaccaggtg catggtgtgg
    12121 ttagtggaac caccggtcgc caacagtgca acgataccgt tcaccaccac tttctcatcg
    12181 atcatcttac cgatcggcat ccattcatta ccattaccgg tcatgcgtgt aacctgacgc
    12241 gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa agaagagcct
    12301 ggcaattgca tccccataaa ttccaccacc atctggttgg tgttggcagt accgtagaaa
    12361 gtacatgttc ccggcgcatg gtaagacgcg gcttctgact ccagtaaggc catgcggtcc
    12421 actttacctt cggcataaag ctggcgaata cgcacttttt ctttatttgg caaaccgctt
    12481 gccatcggtc cagacggcac aaacaccgca ggcaaatgac caaacgacag ggctgccatc
    12541 gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta
    12601 tgggacagcc ccaccgccgc agacatcgct atcacttcgc ggcttagcag cgacaattcc
    12661 attccatcct gcccctgggt gacaccatca cacatcgccg gaacaccgcc cgcaacctga
    12721 ccaaccgcat tcgcttcatg cagggcttta cgaatgattt ctggatagtg ttcataaggc
    12781 tggtgcgcgg agagcatgtc gttataggag gtgatgatgg cgatattgtt acgcaacatg
    12841 cttttcaaag aggctttgtc ttctggctgg caggcagcga aaccgtgtgc caggttaccg
    12901 catgccaact gcgaacgatg aacggtcgac gttttcgctt gttctatccg ggcgagataa
    12961 gcagagcgag tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa caattgtgga
    13021 ttcataaagg ctcctgaaat tgagttgtca gagcaggatg attcacaacg cgtttcattc
    13081 agaggattta tgagtgaaac gcctgtaacc ggagctcata gggcaaaaac gtttcagtca
    13141 gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt
    13201 ctacaatctg tgcaagatca tgttaccggt aaaataacca taaaggataa gcgctgatat
    13261 tactcaaact cattccagga acgaccatca cgggtaatca tcgccaccga ggcaacgggt
    13321 ccccaggttc cggcctgata cggtttcggc gcatcattgt ccatcgccca cgcctcagta
    13381 atggagtcta cccatttcca ggcttcttcc acttcgtcgc gacgtacaaa cagtgcctga
    13441 ataccacgca tggtttccag cagcaaacgt tcataggcat ccgccagatg cgtctgatta
    13501 aaggtttctg aatagctcag atccagcttg gtgatttgca ggttatgttt gtggtcaagg
    13561 ccaggaactt tattcagtac ctggatatcc acgccttcat caggttgcag acggatagtc
    13621 agtttattct gcggcagatc ctgccacgac tctttaaaca gattcagttc aggtgttttg
    13681 aaatagacca cgacttcaga acatttggtc ggcagacgtt taccagtacg caggtagaat
    13741 ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta
    13801 ttgctgcttt tgttcgcgcc ctcttcttcc agatatcccg gcactttttt gccctgggcg
    13861 aagcccgcag tatattgccc gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg
    13921 cgcagggact tcagtacttt cactttttca tcgcggatgc tgtctgcgct caggtcagat
    13981 ggcggagaca tcgcaatcat gcagagaatt tgtagcaggt ggttctgaat catgtcacgc
    14041 atctgaccgg ctttatcaaa atagccccag cgcccttcga tccccacttc ttctgccacg
    14101 gtaatctcaa catgatcaat ggtgcgattg tcccagttat tcacaaacag ggagttagca
    14161 aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc gatacggtaa
    14221 acctggcact cctcgaagta ttcgccaacc tgatcattga tttcctgcga ggtcgccagc
    14281 gacgtcccca gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctcg
    14341 ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatagtg
    14401 atacgatttt tttgatccag catcgcgccg agacggttga atgcagcagt gtcattgaca
    14461 tcgagattac aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt
    14521 tctttcatga aagtttcgag cgcctcgcgg acaactttgg tatatgccgc tttatcccag
    14581 tcagcacgcc ctacgccgat aatccgggtg tccgggttga gctgaccggc tttttccagt
    14641 tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc
    14701 aggtcacagg cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc
    14761 cggtacttaa gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttccga
    14821 gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta cgggaaaagc gcgcaaagtg
    14881 cggcaaaact gataaaaaaa tcatcgtttt ccattcggta aaaatctgac actgctcatg
    14941 ttatgaaaaa aaaataacaa cttttttatc tgcttttgtc attaacacgg cacacaggcg
    15001 taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga
    15061 gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa
    15121 atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag
    15181 tattgctgct ctggcgctgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg
    15241 cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaaa gtctggcgaa
    15301 tggcactccc tatgttaatc gcaacgtcaa tgaagatgac agcgttgaat catacacggg
    15361 gaaaatattt gagtccgcaa tggcaacgct cgatcatgtc cgtcattcac tggataaatc
    15421 tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt
    15481 aggctcttct gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc
    15541 ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg
    15601 agacgtggtg gtgctgattt ctcacacagg aagaacaaaa aatctggtcg agctggcgca
    15661 gctggcacgc gaaaacgacg ccatggtgat tgccctcacc tctgcgggta ccccgctcgc
    15721 ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc
    15781 catggtttct cgacttgcac agctgaccgt gatagatgtg ctggcgacag gatttacttt
    15841 gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc tgaaggaatc
    15901 gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaagcgac tgtcactgtc
    15961 ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc
    16021 ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccaaaa
    16081 ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataatc
    16141 ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct cacggctcgc
    16201 ctgaagatca caaaatgcac gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc
    16261 atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag
    16321 gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg
    16381 aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg
    16441 gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca
    16501 tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca
    16561 aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga
    16621 ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc
    16681 tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg
    16741 ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg
    16801 acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg
    16861 gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga
    16921 cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg
    16981 tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgctg
    17041 ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa
    17101 tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag
    17161 ctattgccat gtcggcaatg tacgcagcta accacctgaa gggcgttacg gcgatcatta
    17221 ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctaccaa
    17281 ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta
    17341 cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc
    17401 tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg
    17461 tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag taagtacgtt
    17521 gccggatgcg gcgaaaacgc cacatccggc ctatagttca atgatagttc aacagatttc
    17581 gaatattctg aagcaaactt gaacttatca tcaggcgaag gcctctcctc gcgagaggct
    17641 tttttatttg atgggataaa gatctttgcg cttatacggc tggatttcgc ccggtttgcg
    17701 agttttcagc aattttagta tccaggtgta ttgttctggt cgcggaccaa caaaaatctc
    17761 gacttcttca ttcatccgcc gctcaatcgt atgatcatcc gcctctaaca gatcatccat
    17821 cggtgggcgc acctgaatcg tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg
    17881 tacaacgcgc gcacggcata ctttcatcaa acgaccaatc gcgggcaacg tcgctttata
    17941 ggtggcaaag aaatccacaa attcgctgtg ctctgggcca tgatcctgat cgggtaaata
    18001 atatccccag tacccctgac gtaccgactg gatgaatggt ttaataccgt catttctcgc
    18061 atgcagacga ccgccaaagc gacgacgcac cgtgttccag acataatcaa aaaccgggtt
    18121 gccctgatta tggaacatcg ctgccatttt ctgcccttgc gaggccatca gcatggcagg
    18181 aatatcgacg gcccaaccgt gcggcaccag aaagataact ttctcgttat tacgccgcat
    18241 ctcttcgatg atctccagcc cttgccagcc aacgcgcggc tgaattttct ccggcccgcg
    18301 tattgccaac tcagccatca ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac
    18361 aatcgcttcg cgttcagctt cactacgttc tggaaagcag agcgacagat tgattaacgc
    18421 acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg
    18481 atcacggaac tttggcggcg ttaaagcgat acccgccatc gctgctacac ccagccatgc
    18541 tccccagtag cgcgggtggc gaaaggattt atcaaactca ggaatgtatt cgctattatt
    18601 ttttttcgtt tccatgcttt tccagtttcg gataaggcaa aaatcaatct ggtgatagtg
    18661 tagcggcgca acttgccccg caccaaataa aaaaagccgg tactgactgc gtaccggctg
    18721 cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg
    18781 aattcgcgac gatcggagcc ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg
    18841 tttacggcct gctggtttat ccatacttca tagtgcagat gcggcccagt tgaacgtccg
    18901 gtattaccgg aaagcgcgat acggtcgcca cgtttcacct tctgtcccgg tttcaccaga
    18961 atcttgcgca agtgcatata acgcgtggtg tagctgcgac catgacgaat agccacataa
    19021 taacctgctg cgccactgcg tttggcaacc accacttcac cgtcacccac tgaaagcact
    19081 ggcgtacctt gtggcatggc gaaatcaaca cctctgtgtg gtgcaacgcg acctgtcacc
    19141 ggattagttc gacgcgggtt aaagttagac gagatacgga actgtttcgc cgtcgggaat
    19201 cgcaagaatc ctttcgccag accagtaccg ttacgatcgt agaatttgcc atcctcagcg
    19261 cggattgcgt aataatcttt accttctgaa cgcaaacgta cgcccagcag ctggctttgc
    19321 tcacgtttac catcaagcat ttctcgagac attaataccg caaattcatc gccttttttc
    19381 agtttgcgga aatccatttg ccactgcatg gctttaatca ctgcgctcac ttcggcgctg
    19441 gttaaaccgg cgtttctggc gctggcaaca aagctccctc cgacggtacc tttcagcaga
    19501 ttgttaaccc actctccttg ctgcatttcg ctggtcattt taaaaccgtt agcggcagta
    19561 cggtcatagg ttcgggtttc acgacgagac acttcccagg tgaggcgctg cagttcgccg
    19621 tccgcggtta atgtccagga gagttgttga ccgattttca ggttacgcaa ttctttgtcg
    19681 gcagcagcga gttgggtgat atcacccata tcaataccat actgattgag aatgctgctt
    19741 agcgtatcgc cagtggaaac aacatattca tgcacgcccg cttcaccggc gattttgtca
    19801 tccagttcgt cctggggaat ggcttcatct tcttgtgcag cttgatcaat cggctcactg
    19861 gcttcaggta agagcgaacg aatttcgttc tgttccagct caatggtttt gacaattggc
    19921 gtggcatcac ggtgataaac atagggccgc cagacagcga cggccagagt aagaacggtg
    19981 agcgacccca acataacgcg gtgtggtcgc ggtaaattat taaacgccag ggcgacagag
    20041 cgggctatct gttgcacgta atcacttcct cattaatctc ctttcaggca gctcgcatac
    20101 tggttggcta attgactcag gaattctgaa tagcttgttt tacccagttt gatattcgtc
    20161 cccaggggat ccaacgttcc catacgaacg gatgtccctc gtgcgacgct ctcaacgacc
    20221 gctggcctga actgtggctc agcaaaaacg caggttgctt tttgctcaac caactgtgtt
    20281 cttatttcat gtaaacgctg cgcgccaggt tgaatctcag ggttaacggt aaaatgacca
    20341 agcggtgtca gtccgaactg tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa
    20401 cctttcccct tgagcggcgc gagctcgtta ccaacctgcg tttcggttga ggccaattgt
    20461 gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac tttgcggcat aagttccact
    20521 aattttccat ggattgcaac cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc
    20581 atgttgaaat cgccgtgatg gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca
    20641 tcatcgccgt gaatactttt catcagtagc ggtttcacat cttcaagctg cgcaatcgtt
    20701 acctgcttcg cttctggtaa tttgcttacc ggtttttgca taaacgcttc catctccggg
    20761 ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta catccgatgg acgcagtgaa
    20821 taatcatgtt ctgaagcgcc gtcaggcagt aaaacctccg tttctgttac accatcagca
    20881 atggcagaag cgatgaaccc aacgggttta agcgaagcga caacggcggc atctgcggcc
    20941 tgtgttgcac ctccccagag agcggcggat aatgctgcga aaagaagcgt ttttttatgt
    21001 aacataatgc gaccaatcat cgtaatgaat atgagaagtg tgatattata acatttcatg
    21061 actactgcaa gactaaaatt aacatgacaa gtctggtttc cctggaaaat gtctcggttt
    21121 cttttggcca acgccgcgtc ctctctgatg tgtcgctgga acttaaacct ggaaaaattt
    21181 tgactttact tgggccaaat ggcgcaggta agtcgacact ggtacgggta gtgctcgggc
    21241 tggtaacacc cgatgaaggg gttatcaaac gcaacggaaa actgcgcatc ggctatgtac
    21301 cgcagaagct gtatctcgac accacgttgc cactgaccgt aaaccgtttt ttacgcttac
    21361 gccctggtac acataaagaa gatattttgc ctgcactgaa acgtgtccag gccgggcacc
    21421 tgattaacgc accgatgcaa aagctctctg gtggcgaaac gcagcgtgta ctattagcgc
    21481 gagcattgtt aaattgcccg caattattag tgctggatga acccactcaa ggcgtggatg
    21541 taaatggcca agtggcgtta tatgacctta ttgaccagtt gcgtcgtgaa ctggattgtg
    21601 gcgttttaat ggtttctcac gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt
    21661 gcctgaatca ccacatttgt tgttccggca caccggaagt tgtttccctg catccggagt
    21721 ttatttcaat gtttggtcct cgtggtgctg aacaactggg tatctatcgc catcatcata
    21781 atcatcgtca cgatttacag ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat
    21841 tgaattatta tttcccggtt ggttagccgg gatcatgctc gcctgtgccg cgggtccgct
    21901 gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc
    21961 attacttggt gtcgcgtttg gtttgttgct ggacgtgaat ccattctatg cggtgattgc
    22021 cgttacgctg ctgctggcgg gcggtctggt atggctggag aagcgtccac agctggcgat
    22081 cgacacgtta ttagggatta tggcgcacag tgccctgtcg ctgggcctag tggtcgttag
    22141 tctgatgtct aacattcgtg ttgatttgat ggcttacctg ttcggtgatt tactggcagt
    22201 gacgccagaa gatctcatct ctattgcgat tggcgtggtc atcgtggtgg ctattttgtt
    22261 ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg gatctggcgt ttgttgatgg
    22321 tgtgaaatta cagcaagtga aattgttgtt gatgctggtg acggcattga cgattggtgt
    22381 agcgatgaaa tttgtcggtg cgttgattat tacttcgttg ctgattattc ctgcagctac
    22441 tgcgcgtcgc tttgcccgta cgccggaaca gatggctggt gtcgctgttt tggtggggat
    22501 ggtggcagta actggcggtt taaccttttc cgcgttttac gatacgccgg cgggtccgtc
    22561 ggtggtgcta tgtgcggcac tgttatttat tatcagtatg atgaaaaagc aggccagcta
    22621 atctatcgct gaacacattt gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg
    22681 ctatctctgg ctggcctgat aagacgcgaa cagtgtcgca tcagacacac tgccagtgtc
    22741 ggatgcggcg cgagcgacca atccgactta cggcatttct ggcggcgtta tgccaaagtg
    22801 attccacgcc cgcgtcgtcg ccatacgccc gcgcggtgta cgctgcaaaa agccttgctg
    22861 aatcaaataa ggttccagta catcttcaat ggtttcacgt tcttcgccaa tggctgccgc
    22921 caggttatcc agacctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt
    22981 gcggtccata taatcgaaac cttcagcatc gacattcaac atatccagcg cctgagcagc
    23041 gatatctgcc gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag
    23101 cagacggttg gcaatacgtg gcgtaccgcg agcgcgacga gctacttcct gcgcaccgtc
    23161 atcactcatc tcaagcccca taaagcgtgc actgcgactg acgatatatt gcagatccgg
    23221 cacctgataa aactccagac gttgcacaat accaaaacgg tcgcgcaacg gtgatgtcag
    23281 cgaacctgcg cgcgtggttg cgccaatcag ggtaaacggc ggcaaatcaa ttttaatgga
    23341 gcgtgccgcc ggaccttcac caatcatgat atccagttgg tagtcttcca ttgccgggta
    23401 cagcacttct tcaacaactg gcgataaacg gtggatctca tcaataaaca gcacgtcatg
    23461 cggttcaagg ttagtgagca tcgcagccaa atcgcccgcc ttttccagca ccggaccaga
    23521 agtcgtgcgt aaattaacgc ccatttcatt ggcgacaatg ttggcaagcg tagttttacc
    23581 caaccccgga ggaccaaaaa tcaacaaatg atcgagggca tcgccgcgca gtttcgctgc
    23641 tttgatgaaa atctccatct gtgaacgaac ctgcggctga ccaacatact cttccagtaa
    23701 tttagggcga atggcgcgat ctgctacatc ttccggcaaa gtggtaccgg cagaaatcag
    23761 acggtctgct tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat
    23821 gtttcactgc tggcgtcagg gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt
    23881 ttatagccta gcgccaccag cgcggcaact gcttcttgtt cagcatcgtc ggtcgccggg
    23941 ctggcaggag acgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct
    24001 ttaaatcggt ctttcatttc aacaatcaag cgttcggcgg tttttttgcc aatacccggc
    24061 agtttcacca gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac
    24121 attccggaga ggatcgccag ggccaacttc gggccgacgc cgttggtttt gatcaactct
    24181 ttgaacaacg tgcgttcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc
    24241 accacaaagt gggtgaaaac gatcgcttcc tgacccgctt cagggagttc ataaaaacag
    24301 gtcatcggca tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt
    24361 tgtttttcaa tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat
    24421 caaagataat gctgtatgat aaaaaaatgc tggatagata tccagcgtag gatgaagaaa
    24481 acttgcgagg tgtcccgatg atctgaaaaa tggcacagta taatttattc aacaggttat
    24541 attggaagca aatatttgaa tattacatat tcagtgaaga aatgcgtaat aaaaatatac
    24601 attgcgactc ctgaaaaaat aattttttta tgctattacg tatattcgta tctatttcaa
    24661 tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga tatcgtcttt
    24721 acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat
    24781 cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt
    24841 cccctttcaa ccatcaccac g
//
LOCUS       DADLXX010000049        24830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLXX010000049 DADLXX010000000
VERSION     DADLXX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.049"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..373
                     /locus_tag="JNX27_001985"
     CDS             <1..373
                     /locus_tag="JNX27_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883210.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="JNX27_001986"
     CDS             484..1407
                     /locus_tag="JNX27_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7883211.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVKGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNX27_001987"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNX27_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY7883212.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="JNX27_001988"
     CDS             2451..2744
                     /locus_tag="JNX27_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883213.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNX27_001989"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNX27_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7883214.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="JNX27_001990"
     CDS             complement(4186..4638)
                     /locus_tag="JNX27_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7883215.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHPPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /locus_tag="JNX27_001991"
     CDS             complement(4691..6025)
                     /locus_tag="JNX27_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7883216.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="JNX27_001992"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="JNX27_001992"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY7883217.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNX27_001993"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNX27_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7883218.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNX27_001994"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNX27_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7883219.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNX27_001995"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNX27_001995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7883220.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNX27_001996"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNX27_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7883221.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /locus_tag="JNX27_001997"
     CDS             complement(13037..13327)
                     /locus_tag="JNX27_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY7883222.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNX27_001998"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNX27_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7883223.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNX27_001999"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNX27_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7883224.1"
                     /translation="MTTSMFNVKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="JNX27_002000"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="JNX27_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7883225.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="JNX27_002001"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="JNX27_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7883226.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="JNX27_002002"
     CDS             complement(17349..17846)
                     /locus_tag="JNX27_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7883227.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPSRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="JNX27_002003"
     CDS             complement(17843..18205)
                     /locus_tag="JNX27_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7883228.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="JNX27_002004"
     CDS             complement(18195..18542)
                     /locus_tag="JNX27_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7883229.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="JNX27_002005"
     CDS             18651..19064
                     /locus_tag="JNX27_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883230.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20577)
                     /locus_tag="JNX27_002006"
     CDS             complement(19111..20577)
                     /locus_tag="JNX27_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7883231.1"
                     /translation="MTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCT
                     PARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGT
                     GECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNR
                     AVDFLQQPVRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPE
                     HHRLWAQAMLSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDH
                     GEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEK
                     PEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDEL
                     YDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPR
                     WMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20588..22303)
                     /locus_tag="JNX27_002007"
                     /pseudo
     CDS             complement(20588..22303)
                     /locus_tag="JNX27_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22470..23363
                     /locus_tag="JNX27_002008"
     CDS             22470..23363
                     /locus_tag="JNX27_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7883232.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23360..24174)
                     /locus_tag="JNX27_002009"
                     /pseudo
     CDS             complement(23360..24174)
                     /locus_tag="JNX27_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(24174..>24830)
                     /locus_tag="JNX27_002010"
     CDS             complement(24174..>24830)
                     /locus_tag="JNX27_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY7883233.1"
                     /translation="VGITEPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGG
                     GLIDQVLPQNWIPMFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAE
                     VKLYSKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALHDM
                     SQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV
                     "
BASE COUNT         6251 a         6650 c         6184 g         5745 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaaaggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg ggatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc cattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgctactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gagataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctagcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccacagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcctg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt acgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggctcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgccagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg
    19681 aatacgggtg atgtcgtcat acatcgccgc tcctttactg atcagcttat gtgcgcccat
    19741 catttcaccg tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa
    19801 ggcattgatg acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg
    19861 atggtgataa agcccgtcat caccgactgg cgatagcatc gcctgcgccc ataagcggtg
    19921 atgttccggt ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc
    19981 agcgtatttc tctaaatact ccaccggaca ggtgaacggg tgattcggct catcatacga
    20041 aaccaccatc aggaagggtt cctcggcgcg cacgggctgt tgcagaaaat ctaccgctcg
    20101 attgctgatg cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc
    20161 gacgctgttt aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata
    20221 gttcgcccca tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa
    20281 atagtcatga ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt
    20341 aaagtagcgc cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc
    20401 ggactggtta gcgtagatac cggtgaatag tccggcgcgt gcaggcgtac aaaccggtga
    20461 acaagtgtag gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt
    20521 attcagcggt ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg
    20581 gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat
    20641 gccgccagct gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca
    20701 tcgctaacca gcgcgtaccg tagccgccga attcagccaa cccgacatag acgccaatca
    20761 tcgcgaacag gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg
    20821 caaacgcatc tttgaaggtg aacggcgtgg cgcgcgcttt gataaaaccg atcaccagca
    20881 tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact
    20941 tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg
    21001 ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt
    21061 tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc
    21121 acggcgctac caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga
    21181 ccagctgctg cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata
    21241 atgtactggc gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg
    21301 cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca
    21361 tgtctgcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta
    21421 ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca
    21481 ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt
    21541 tcaccagaat taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga
    21601 ctgagtttaa tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc
    21661 ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa
    21721 taccgttgat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa
    21781 ttcccgccag accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta
    21841 gcgattcccc gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg
    21901 gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt
    21961 tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg
    22021 gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca
    22081 ccgacatgcc gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt
    22141 tggttaacat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat
    22201 agccttgttg cgatccggta tctgttttgc gaaccttcca ctaggtgatc accgccacca
    22261 gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta
    22321 tgtttatgtc ccggacgcat gaatataggg agaggtaaaa tgtgtcgggt caggcagtca
    22381 gattcttaaa atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg
    22441 tgagcataat tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa
    22501 aaaacgaaac tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa
    22561 tttctctgat ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc
    22621 gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat
    22681 gtgggaaaat tcttttaaag aatggcgaac aaataactct gcatggcaac tgtattatat
    22741 ttttaaagcc aatggatatt cactcttatc actgtgaagg tttagtcttg gaacagtact
    22801 ggatggaatt tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt
    22861 ataatggcga aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag
    22921 aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt
    22981 gtcttatgtc cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg
    23041 ccactttaca tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc
    23101 cctgtagcga agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag
    23161 aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact
    23221 cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct
    23281 ttaaacataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
    23341 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
    23401 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg
    23461 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
    23521 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
    23581 caggccgtag tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat
    23641 ccggctttca ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag
    23701 acgacgcgtg gcttctgcga caattgctgc cgggttggag tagttgagca tctaggcgtt
    23761 tggtgaatat ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc
    23821 gtacgctatt ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat
    23881 tttttcatcc agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc
    23941 aacgtcgctg aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt
    24001 ttctttaagg atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc
    24061 ataaaatttc agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat
    24121 gcctggcgta aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact
    24181 gcctccgtaa tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt
    24241 tcacgcaatt gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag
    24301 acgccgtgcg ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc
    24361 agtgcaatac gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc
    24421 agggcttgca ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt
    24481 tggcctcgcg aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt
    24541 cccggcgtgc acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg
    24601 gtaaagcaca acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc
    24661 gggatccagt tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca
    24721 ccaaagagat acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc
    24781 aacggtgaaa tgaacaggaa ggtaaattcc agcggttcgg taattcccac
//
LOCUS       DADLXX010000050        24752 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLXX010000050 DADLXX010000000
VERSION     DADLXX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.050"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(47..4664)
                     /locus_tag="JNX27_002011"
                     /pseudo
     CDS             complement(47..4664)
                     /locus_tag="JNX27_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4757..5404)
                     /gene="rnt"
                     /locus_tag="JNX27_002012"
     CDS             complement(4757..5404)
                     /gene="rnt"
                     /locus_tag="JNX27_002012"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7883234.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5507..5914)
                     /gene="gloA"
                     /locus_tag="JNX27_002013"
     CDS             complement(5507..5914)
                     /gene="gloA"
                     /locus_tag="JNX27_002013"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7883235.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5995..7092)
                     /gene="nemA"
                     /locus_tag="JNX27_002014"
     CDS             complement(5995..7092)
                     /gene="nemA"
                     /locus_tag="JNX27_002014"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY7883236.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7129..7728)
                     /locus_tag="JNX27_002015"
     CDS             complement(7129..7728)
                     /locus_tag="JNX27_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7883237.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLIFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7831..8070
                     /locus_tag="JNX27_002016"
     CDS             7831..8070
                     /locus_tag="JNX27_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7883238.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8119..9015
                     /locus_tag="JNX27_002017"
     CDS             8119..9015
                     /locus_tag="JNX27_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7883239.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGMLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9096..9617
                     /gene="sodC"
                     /locus_tag="JNX27_002018"
     CDS             9096..9617
                     /gene="sodC"
                     /locus_tag="JNX27_002018"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY7883240.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9618..11600)
                     /locus_tag="JNX27_002019"
                     /pseudo
     CDS             complement(9618..11600)
                     /locus_tag="JNX27_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11497..12384)
                     /locus_tag="JNX27_002020"
     CDS             complement(11497..12384)
                     /locus_tag="JNX27_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7883241.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIHAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALVQGHAGAMALCV
                     TQYHCHVSGADGCLLFESG"
     gene            complement(12387..12623)
                     /locus_tag="JNX27_002021"
     CDS             complement(12387..12623)
                     /locus_tag="JNX27_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7883242.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            12824..13258
                     /gene="slyA"
                     /locus_tag="JNX27_002022"
     CDS             12824..13258
                     /gene="slyA"
                     /locus_tag="JNX27_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7883243.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13305..13772)
                     /gene="slyB"
                     /locus_tag="JNX27_002023"
     CDS             complement(13305..13772)
                     /gene="slyB"
                     /locus_tag="JNX27_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7883244.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14046..15155
                     /gene="anmK"
                     /locus_tag="JNX27_002024"
     CDS             14046..15155
                     /gene="anmK"
                     /locus_tag="JNX27_002024"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7883245.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15253..15582
                     /gene="mliC"
                     /locus_tag="JNX27_002025"
     CDS             15253..15582
                     /gene="mliC"
                     /locus_tag="JNX27_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7883246.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGFSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15641..16297
                     /gene="pdxH"
                     /locus_tag="JNX27_002026"
     CDS             15641..16297
                     /gene="pdxH"
                     /locus_tag="JNX27_002026"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7883247.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16426..17700
                     /gene="tyrS"
                     /locus_tag="JNX27_002027"
     CDS             16426..17700
                     /gene="tyrS"
                     /locus_tag="JNX27_002027"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7883248.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17762..18628
                     /gene="pdxY"
                     /locus_tag="JNX27_002028"
     CDS             17762..18628
                     /gene="pdxY"
                     /locus_tag="JNX27_002028"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HAY7883249.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHVLGIVRQVK
                     AANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVN
                     NVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMR
                     QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRI
                     AKPEHYFSATKL"
     gene            complement(18672..19277)
                     /gene="gstA"
                     /locus_tag="JNX27_002029"
     CDS             complement(18672..19277)
                     /gene="gstA"
                     /locus_tag="JNX27_002029"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7883250.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19383..20885)
                     /gene="dtpA"
                     /locus_tag="JNX27_002030"
     CDS             complement(19383..20885)
                     /gene="dtpA"
                     /locus_tag="JNX27_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7883251.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(21496..22131)
                     /gene="nth"
                     /locus_tag="JNX27_002031"
     CDS             complement(21496..22131)
                     /gene="nth"
                     /locus_tag="JNX27_002031"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7883252.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKERVDI"
     gene            complement(22131..22826)
                     /locus_tag="JNX27_002032"
     CDS             complement(22131..22826)
                     /locus_tag="JNX27_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7883253.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(22830..23450)
                     /gene="rsxG"
                     /locus_tag="JNX27_002033"
     CDS             complement(22830..23450)
                     /gene="rsxG"
                     /locus_tag="JNX27_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7883254.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23454..24512)
                     /gene="rsxD"
                     /locus_tag="JNX27_002034"
     CDS             complement(23454..24512)
                     /gene="rsxD"
                     /locus_tag="JNX27_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7883255.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24513..>24752)
                     /locus_tag="JNX27_002035"
     CDS             complement(24513..>24752)
                     /locus_tag="JNX27_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7883256.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         6113 a         6406 c         6454 g         5779 t
ORIGIN      
        1 tgaacgtagc agggaaccac gtctttcagg gcgtggagga tgtcaactat ccccaatcca
       61 gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag cgtaaacatc ggcgtttgcc
      121 ggaccggaag atcattcact tctgttagcg taaagcgcag ccttggttcg cgacgcagtg
      181 cggtggtcag cgcctggaaa atctctgggg cgagtaattc ctccttttcc tgccagacca
      241 gcattttttt gccgccttgc gccagataga gtaacaattt gccgccagaa acgaccacca
      301 gcgcacccgc ccgacgcgtt ggtaccagcg ctgccggatg tgcaggccag ggcagaagat
      361 ttccccacac attagccgga tcgttggcgg agagcgccac tggtgtatag tggcgtgttt
      421 gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt cagacgttca gcgaattgcg
      481 cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct tccatacttc
      541 gacaaagtgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac
      601 gactgatgat gccgtagcgg tcgagcatat tttccgccag cgccagcatc ctttcggtat
      661 cgtttagtgg ctccacctgc aataacgacc agcgtccagc cagatttggt gtgttgtaag
      721 atacccgcgg cgagacgggt tgtgcataga caggacgccc acgacgagcc cggtgactgc
      781 ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa
      841 tatcgctggt aatgacgcct tgccagacca gcgcccataa cgcctcttgc agtgccgagg
      901 aatcaaccga ttcgccgatt ttatcgcgta tccgttggct gatttgttgt gcaaaccagg
      961 ctcctccgtc agccagaaca gcgattatcg cttgctgcag cgccgaacga ttcgcctgat
     1021 ccgcttcggc gggagtgaac gattctgcgg catatttctg tagatgcagt gccaccaggc
     1081 cgtcatcttc acccagcttt tttttgtccc gaccagataa ccgctccggt tgccagtaat
     1141 tcatcgagca tttctggcga atagtcgcgt acgcgggcag gcaggatctg gctttcccag
     1201 agtgaggcgg gtaaaccgac tccggcaagc tgttcgatca cccgcatcac gccatctacg
     1261 ccctcataaa cgcctggcga tgttgaggca aagagcgccg ggctaccatc ggtggcgggt
     1321 aatacgccct gacgttccag cagcaatcgc gcataggtcg tggctgcaac gggacgcgtc
     1381 gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac gaaatacttc atcgctcacc
     1441 cagatgtctt gttgcagatt catcaccaga ccctgttcac gcagttgctg aagctgctct
     1501 tcgacaatgg caataccgag actaaactca tgagccagtt gttcagccgt gaccagagca
     1561 tgagcccgga gatagcgcag aaagaggtcg cgaagcgggt aactgactct atgtaaataa
     1621 atctctggca atgactctgg tagtcgtacg ccgagggcat cacgcagcct ggcggcatca
     1681 tccatacagg caagacgctc ctgtccgcta atcatcgcag ggaagattcg ttttactgca
     1741 agaagatttt ccagatacga cgcaacctct tcactgctgc ctgtatgccg ttgcgccagg
     1801 tcttcaacgg tcattggccc cagttcgcgc agcaggtcga acaatccttc ttcacctttc
     1861 gctcttctgc caggagccag tcgttgcaac tcttcttcca cctggcgaat gacctgcggg
     1921 tcgagtaatt cccccggatc gacctgtccg agtagattgc gcagtaactc gctgtccagc
     1981 gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc tctggtacat aaattccgcg
     2041 acatagccga acaataaact tgtggcgaaa ggcgagggcg tagtggtcgt tacatcggat
     2101 atttgaattt cgccaccgtt caggcgacgc atcaaacgtt ccagtgcggg aagatcataa
     2161 acatcttgca gacattcgcg tagggtttcg agaatgaccg gaaaatccgg atatccctga
     2221 gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc aaagcggggt gcgatggccc
     2281 ggcgtgcggc ccggcattaa taatgcccgc gcggcgcatt cgcgaaaacg ggcggcgaaa
     2341 agtgccgagc tgcctaccgc ctcgcggaca atttgcagca acttttctgg ttcaaacaaa
     2401 aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa tgcgcgcaac aatgccgtca
     2461 tcactggcga ccacagacgc gtcagcgccc catagggcat gtattcgccc ggcaatcgcc
     2521 agcgcccagg gttcatgcac ccgtcttcca tagggagagt gcaaaataat acgccagtcg
     2581 ccaatttcat cacggcaccg ttcgaggacc agatggcgac tccccggaac gatgcccgtc
     2641 gcgttgcgtt gctcgtcaat caacccctga atattggcgt tcgtattttc ttctgccagc
     2701 cacgggggaa tagtcccgga aaagaacgcg ccatccgcca gtaaatgaag aaaatcgccg
     2761 atcatctcgc ctaattcagc cggacgtccg ttaccttcac cacgccagaa ggggagccgg
     2821 gcagaacgac ccggagcagg agtcacaatc acctgatcgc gggtgatttg ctggatccgc
     2881 catgaggtag cgccgagagt gataatgtcg ttcacccgcg actcatatac catctcctca
     2941 tccagttcac ccacccgccg cgaaccggcc ttttcttcac cttcgggtaa taacacgcta
     3001 tacatgccac gatccggaat ggtgccgccg ctggtaaccg ccaacaattg agcgccaggt
     3061 cgggcggtca atatcccggt ctccctgttc cagaccagtt tggggcgaaa agcagaaaaa
     3121 tcgccagagg gatagcgccc ggaaagcata tccagcgtgg cgtcaaaaac acgtcttggc
     3181 agatctttcc acggtgcggc acggcgtacg cgggagtacc attcgtctac ctgtaatgca
     3241 tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt cgagaggatt atgcggtggt
     3301 gtcaggtttt ccagcctgcc tgcgaacata cactctacaa tgactgcgga atcgactaaa
     3361 tcacgccggg tacgggggaa aaacagccct ttagatacac cgccaacctg atgtccggcg
     3421 cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg ttgcctcctg aatcaccaga
     3481 tccaccgcgc ccatatcaat ccccagttca agactggagg ttgcgaccac gcagcgtaat
     3541 tcacccgatt tcagcgcctg ttcggtgatt gctcgttgtt ctttagagac ggagccgtgg
     3601 tgtgagcggg caataaaaac gtcgctactt tgtacacggt tagaggttgc gccggaggtc
     3661 gactcgaaat gggccgcatc aacggcgata gacggggaac gctgtaagcg tgcggcgtaa
     3721 agctcattta atcgtgccgt cagtttttcc gccagcccgc gtgaattagt aaagacaatg
     3781 gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt caatatatgg ccagatggag
     3841 ccttcccggc cggcatggct gtcttcgccg gtgccgctgg cgaccgatga gacatcatcc
     3901 atattggcga ccggtacgac aattcgtatc tgcggatggc gcattgcggg cgggttgact
     3961 accgtaaccg ggcgatcgcc accaagaaat gctgccacat cgctggctga gcgcacagtg
     4021 gcagaaaggc caattcgctg tgctgaggtg tggagcagcg catcgagccg ctccagactt
     4081 aacgccagat gcgcaccacg tttactgccc gctaccgcgt ggacttcatc aataattacc
     4141 gtttcgacgc cgtgtagcgt ttcgcgcgcg cgggaggtca gcatcagata gagtgattcg
     4201 ggtgtggtga tcagaatatc cggcggatta cgggtgagtt tgctgcgttc ctgtgcaggc
     4261 gtatcgccag tacggatccc tacgcgaaga ttgacttccg tttcgccgcg ccgccgccgt
     4321 tcatcggcaa tacccttcaa cgggatctgc aagttgcgct gaacgtcggt gcccagggct
     4381 tttatcggtg aaatatagag gatgcgtgag gttttacgct tatgcgcctc gcgggtatct
     4441 tcgccgcctt cgcggaagag ccgatcgagg gcgtagagaa atgctgccag cgttttcccg
     4501 gagccagtcg gtgcaatcac cagcgcatgt tcgcctcgcg ccgccacatg ccaggtttgc
     4561 gactggacag cggtcggctg tttaaaggcg cgaagaaacc agtcgcgggt cgccggtgaa
     4621 aacacgtccg gcaggagcga tgaagggtct ggattatctg ccattaacgc tattccccgt
     4681 gaatatcatg cttgatgcgt ggaatagcct cactatagat gcctgaatca ttgcatgtca
     4741 tcaggcatcg actcgattac acctcttcgg cggcaggtag cggccagcct cccagacgtt
     4801 tccagcggtt gacgatttca caaaacagca cagcagtgcg ttcggtgtcg tacagcgcgg
     4861 agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg gcaagccttt gacaatacgg
     4921 tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa agtggcgaaa gggtggaacg
     4981 ggttacgttt cagcgaggcg cgttctgcgg cggccatcat aaagctgtga tcaaaattgg
     5041 cgttgtgcgc caccataatg gcgcggttac agccgctcgc tttaatacct ttacgtacaa
     5101 ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc gccgcgatcg ggatcgttcg
     5161 ggtcaatgcc gttgaaggcg agggcttctg gttgcaaatt tgcgccgaca aatggttcga
     5221 cgtggaaatg taatgtggtg tccggcatca gccagccttg ttcatccatt ttcagggtga
     5281 tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa tccggctgtt tcaacatcga
     5341 tcacaacagg ataaaaacca cgaaaacggt cgcacagacc ggtaagttga gcgttatcgg
     5401 acatctgatt ctcttaatac ggataaattg cagcgcgcat tatgacaaat attacttgtc
     5461 gatgcagtaa agatgcgggc gcgatgagtt cacgcccggc aggagattag ttgcccagac
     5521 cgcgaccggc gtctttctct tcgattaact caattttgta accgtccgga tcttccacaa
     5581 acgcgataac cgtagtaccg ccttttaccg gacccgcctc acgggtcacg ttacccccgt
     5641 tttgacggat tttttcgcac gcttcagcgg cgttatctac gctaagcgcg atgtgaccat
     5701 aagcagtgcc gagttcgtat ttatccacgc cccagttgta ggtcagttca atcaccgctt
     5761 cttcggtttc cgggccgtag ccaacaaacg ccagtgagta tttgtattcc gggttttcgc
     5821 tggtacgcag cagtttcatg cccagcactt tggtataaaa atcgatggag cgttgcaaat
     5881 cgccaacgcg cagcatggta tgaagaagac gcatcttttt atcctcaaaa tggctaacag
     5941 attcaaaatg ttgttttagt atagcggcgg ctttacgccg ctcgcaatgt tggattacaa
     6001 cgtcgggtaa tcggtatagc cttccgcgcc gccaccgtag aaactttcgg cacgctgtgg
     6061 gttaagctca gctttgagct gcaagcgggc gaccagatcc gggttcgcaa tccagtcacg
     6121 accaaatgcc accgcatcaa ttaacccttt gccgatcagc gtttcagctt tttctactgt
     6181 gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg cgtacttttt cgcggaacgc
     6241 atcagtatac ggttcacccc ccgcccaatc tggttctgac atatgcagat aagcaatgcc
     6301 gcgtttaccc agttgttcaa tcagatacag tgcatcggct tcttcattcg ggccgttatc
     6361 tgtgttctgg aaagtaccga ttggcgaaac gcgaatgcca atgcgatcgg caccccattc
     6421 ttcaatcccg gcatcgacca cttccagtac caaacgtgcg cgattttcca cgctgccgcc
     6481 gtactgatcg gtacgatggt ttgaagaagg ggaaaggaac tgatgcagca aataaccgtg
     6541 agcagagtgg agctctacca gatcaaaacc ggcttcacgc gcgttagcaa tggcctgacg
     6601 gaaatcattg acgatacccg gaatctcttc cagttcaagc gcacgcggca tggatgtttc
     6661 aacgcggatc gcccgaccat tttcatcgcg cagagaagta cgtgttcccg cgctaagtgc
     6721 tgaaggcgct accggtgcct gaccgccagg ttgcaggctg gcgtgagaaa tgcgtccggt
     6781 gtgccacagc tgcaaggcca tatgaccatt ttcagcatga acgccagcgg tgattttttt
     6841 ccatgcggca atttgctccg gactatggat gccaggcgca cctgcatatc cttttgcctg
     6901 ggcagaaatt tgcgtggctt cactaataat caaaccggca ctggcacgtt ggcgatagta
     6961 ttccgccatc aacggggtag gaatgtcacc cggttcaata ctgcgcagac gcgtcagcgg
     7021 tgccataaaa atacggtttg ccgccgtgat cgcgcccact ttcagtgggg aatacagttt
     7081 ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa aatgcctgct aaacggcagg
     7141 cgtcgcaata atgtttttta catgggccag cgcgttttcc agtggctcga aactgcgcga
     7201 aattttggcc tgcagattcg cgccaagcca tagtgcgtaa agcacttgtg cctgttgtag
     7261 cggttcgcca caaaagatta aacaatggtt ctcacggcca ttttccagag cctgcgagag
     7321 cagggcgatc acgccgcgag cacctttatc catcgcgctg cgcatatctt ctgacagatc
     7381 gcacacttcg gcagagagtt ttactgtcag gcaaccactg atggttccat gttggcaaaa
     7441 ctggttcagt gtttgctggt aataagccag tatgcggtcg cggtagttac cttcgccgga
     7501 ttgcagcaac tcagccagtc gctggtgata tgcggcgtaa tgacgctcaa gcatggcaac
     7561 gccaaacgct tctttagagc gaaagtagtg atagaaggac cctttcggca cttcagcggt
     7621 ttttagtaat tcgcttagcc ccatcccggt gaatccacgt tgcaggcaaa gttgctcgcc
     7681 cgtcgccaag agatgttcgc gagtatcatg ttcggtgtgt ttgttcatgc gttggagtgt
     7741 agtagaccag tcggtctagt gcaagcaaag ttgctacttt ggacattaac agcttcaata
     7801 atggtaataa tcttatctga ggagggcgag gtggcggagc aattagagtt ctttcccgtc
     7861 cagagcccgt gccggggaat ttgccagtct gacgaacgcg ggttttgtcg cggctgtttt
     7921 cgtagtcggg atgagcgttt taactggaat aaaatgagcg atggcgaaaa acaagaggtg
     7981 ctgcgccttt gtcggcagcg attaatgcgc aaattacgcg caaataagcc agcgtcatca
     8041 gacgaaccgg agcaaccatc actcttttga acctaatctt gcgtatactc ctctcatatc
     8101 tgataagagg aagcgattat ggttcagcgt attactattg cgccggaagg cccggagttt
     8161 tcccgttttg tgatgggcta ctggcgattg atggactgga atatgtccgc ccgccagctg
     8221 gtcagtttta ttgaagagca tctggatctc ggcgtgacca ccgtggacca tgctgatatt
     8281 tatggtggct atcagtgcga agcggcgttt ggcgaggcac tgaaactggc acctcacctg
     8341 cgtgaacgga tggaaatcgt cagtaaatgc ggtatcgcga cgaccgcgcg tgaagaaaac
     8401 gtcattggtc attacatcac tgaccgcaat cacatcatta agagcgccga acagtcgcta
     8461 attaatctcg cgaccgatca tctggatctg ctgttaatcc accgaccaga cccgttaatg
     8521 gatgccgatg aagtggcgga cgcgttcaaa catctgcatc agagcggcaa agtgcgtcat
     8581 tttggcgtat cgaactttac gcctgcgcaa tttgccctgt tgcaatcacg tctgccgttt
     8641 acccttgcca ctaatcaggt ggaaatatcc ccggtgcatc agccgttact gctggatggc
     8701 atgctcgacc aactacaaca actgcgtgtt cgtccgatgg cgtggtcctg ccttggtggt
     8761 ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg atgaactggc tgtggtggcg
     8821 gaggagttaa acgcgggctc gattgaacag gtggtttacg cctgggtatt acgtttaccg
     8881 tcgcagccgc tgccaattat cggctcaggt aaaattgagc gcgtacgggc agctgtcgaa
     8941 gcagaaacac tgaaaatgac ccgtcaacaa tggtttcgta tccgtaaagc tgcactgggg
     9001 tacgacgtac cgtaagcact tttaggaata gccgccgttc aaaaatgtgt cactggttta
     9061 cacttattca ggaatgcaca atgaacggag gtcctatgaa acgttttagt ctggccattc
     9121 tggcgctggt tgttgcgacc ggcgcacaag ctgccagtga aaaagtcgag atgaacctcg
     9181 tcacgtcgca aggggttggg cagtcaattg gtagcgtcac cattactgaa accgataaag
     9241 gtctggagtt ttcacccgat ctgaaagcat taccccccgg tgaacatggc ttccatattc
     9301 atgccaaagg aagctgccag ccagccacca aagatggcaa agccagcgcc gcggaatccg
     9361 caggcgggca tcttgatcca caaaataccg gtaaacatga agggccagaa ggtaccgggc
     9421 atttaggcga tctgcctgca ctggtcgtca ataatgacgg caaagctacc gatgccgtca
     9481 tcgcgcctcg tctgaaatca ctggatgaaa tcaaagacaa ggcgctgatg gtccacgttg
     9541 gcggcgataa tatgtccgat caacctaaac cgctgggcgg tggcggtgaa cgctatgcct
     9601 gtggtgtaat taagtaatta cgaggccagc gtaccttgcg gtggtgcttg ctcaagttgc
     9661 gaaagcgagc agtacagccg ccagacaatt gccgccagct cacgggcggc aggttgatga
     9721 tgacgggcaa ggctgtcgca aatccgctgt aattcttcaa gtgtggccgc cagtgatttt
     9781 tgctgaacgc cacgctcact catcactccc cgcaacagtg aaatacagtt atcccgtact
     9841 cgcgataacg gatcggaacg cgattcccag tcgcgcaatt gccagacaac atgagaacag
     9901 ttcagcagca ctacacccca gcgtaataac caacggcgag ccagcgcatc ctggctgtta
     9961 ctcaactgac tgacgtgatg ataagtgagc gattcaaatt cgctttcact cagtgttgga
    10021 tggcggctta gctgatcgac aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta
    10081 cgagcatccg atcctggacg cagaatggcg aacgctagcc aagccagcgc aacgccaacg
    10141 atttttgcca gattatcgtt aagaaaatca gcaaaatcat acaccggggg attagtgaca
    10201 gcgataaacg aacccataaa aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc
    10261 tgcaatttaa gaagctgcat tgtcgccagc agtggaaaga gaaacagtaa aaattgccac
    10321 agatcgctaa tctggaccat caggccaaat ttgaccacaa agctgaatag cgaaagtaac
    10381 accagcgtgc gcatcagcag tgacaacgac ttaaacggtg ctgcgacggc ggagtagagt
    10441 acgcagctaa ttgctgccag cgttaatgca ttggcaccgg catcccattg cgatgcaata
    10501 ctccatgcgc caatcatcat caacgtacaa aatgtacgca gcccgctcca catagcttcg
    10561 gcgttgtcgg tatgacgagc cagagcggat gtccgtgcga gtctggcatg atcatctaca
    10621 ccgctttgca gacgatgtaa ttcctgacta ctttgcagat aaaggcggca aaaataacgt
    10681 agtcgctgcc agaaggcgac gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg
    10741 ataatacgtg cgacggtgta agcatctgtt tgcgaactgg cgagcgccgt cagtaattgt
    10801 tcgagaattt ctcgcgtggc acctggcggt gagggccagt tgagcaacat acgtcgcagg
    10861 ctggagatga cactggtcat acgtaattgc tggtggagta gcgcattaag gcgcgcgttt
    10921 tgctggcgaa aacgatagtg gctccagaaa gcctggatac gcagcaaatt catggtcagt
    10981 atctgcccaa tcaccccctc atgtgctgca cgaatggcat cgtttgtttc aggctgccag
    11041 agtaaactgg catgttccag taatcgggcg tgcatgtttt tcaatgcagt taaaagggca
    11101 gtagcatcgg aactgctagg caggatcatc atcatcatgc cgccgcacaa aataccgact
    11161 atcacctcgc aaacgcgcgc ctgagcgata tcccacagct ggctggcctc agtaatatta
    11221 accatcggaa aggcgatgat ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg
    11281 ttattcgtga agtgcgcaca ggcctaggta caaaagccaa gccacgccga catgctcaat
    11341 agaaaaaacc acggctcatt gagcgtatgt ccggcaagaa gcagtgccgc aatggctccc
    11401 agtaaactgc cagcgatgcg tccgaggctt ttgctgatga caccgccaac ggtgggaaag
    11461 ctaaccactg cagccgaggt catcgcccaa tagggttcat ccagattcaa ataataggca
    11521 accgtcagcg ccagacacat ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc
    11581 gtggccctga accagcgcaa agcggacggg aacgcgttgg gcgagacgaa cccaggggac
    11641 gttaggtttc acatccggaa taaggcttga gtcactttca acgctctgat cataaatcgc
    11701 ccgaccgata ctggaaacgt gaccctgtaa cgttttgtta tcactataga gggtaatttg
    11761 tgctggtgca ccttcgcgga tatggcgcaa cttggtttct tcaaaataac caatgacgta
    11821 aaacgagtgg ctgtcgacaa gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat
    11881 gcgggtagtg aggttagtca cccatccact caccggggcg cgaatttccg tttgcgccag
    11941 ttgccattgt gtctgtttca gcgtcgcctg tgcggcatta acactggcct gcatcgcttt
    12001 aacattgagg ttagcggtat cgagttcttc ggcaaaaata aaattttgtg agagatgacg
    12061 acgacgattc gcttcgttat tggcttttgc gaggtcagat tgcgctttcg ccagctgagc
    12121 ctgtgcgttc agctcggcga tctgaaaggg cgttttatcg atcgtgagca gaagttcccc
    12181 cgcgttaacc agttggttgt cttttatatt cagctcaaca atacgtcccg acacctgtgg
    12241 ggtgatagaa acctgttctg cgtgtatttt cccgtcgcgt gtccacggag attgcatgta
    12301 ataattccac agccaccatc cggcaagaac cgcaactatc gctacaatga ttgttgagaa
    12361 atacttaatt gttttaattg acatagttac cacgcaatca gtattgccag agcaaggcaa
    12421 acgcaaatcg caaacagcga taaatccatt aacaagggat gccagatgtc accggcgtag
    12481 atccagccac gaagcagacg gtgtacgaca agccagatga cgaatccaaa cgcgaatgct
    12541 ttgaaaaacg gaggaaaata gacggacgca ccgaacacca gatcttgtaa gggcaatcct
    12601 gttgcattga gcataaactt cacaaagtag attcctttac gaccgaatat attgcgttag
    12661 attaataaat attctttaag tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga
    12721 cggtaaccaa atgcagcaat acatttgttt tagcaataca attgctgcac actattctaa
    12781 aagccgcata atatcttagc aagctaatta taaggagatg aaattggaat cgccactagg
    12841 ttctgatctg gcacggttgg tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc
    12901 gctggagtta acacaaaccc attgggttac gttacacaat atccatcagt tacctccaga
    12961 ccagtcgcaa attcaactgg caaaagcgat tggcatcgag cagccatcac tggtccgtac
    13021 tctggaccaa ctggaagaaa aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg
    13081 ggctaaacgt attaaactga cggaaaaggc agagccactg atcagcgaaa tggaagctgt
    13141 tattaacaaa acccgcgcgg aaatattaca tggcatctcc gcagaggaac tggagcaact
    13201 gattacgctc atcgcaaaac ttgagcataa tatcattgag ttacaggcca aagggtgaaa
    13261 tgaagggggc gtgtggtcag taacctgacc acacgcaaac ttatttagcg cggagaaacg
    13321 gtcacctgac tgccattgct ggccagtacg acacgttggc ccggagagaa acgagtgttg
    13381 ccttgtttct gtaccaccat gatggtatta ccatcgtctt tacgaatttc cagctcgaca
    13441 ccctgcgttt tgttcattgc actctgtacg ccctgaccag ctacgccacc tgcaacagcg
    13501 cctgctgcag tagccagaga acgcccggtt ccgccaccaa cagtattccc caggaaacca
    13561 ccaagaacag caccgccaat tgcaccgata acgttggaat catcaccgcc ctgaatctgt
    13621 accggacgta cgttaacgat ggtgccatag ctgacattct gtacttgttt cgcttcagaa
    13681 gcggtataaa catcccctga cagggtgtcg ttattaacac aaccgacaag agacagacct
    13741 accattgaaa caaccaatac gcgtttaatc actgaaaaat ctcctgttca ccatgaaacg
    13801 ccacgcaagc atccctcatg gtcaaagtat atggcatatg tgaatccatg gttacatgtt
    13861 ctaccaaata tgagcaaatc ataatgaaag taggatcaac caattataaa caaaacattc
    13921 atgatgatta ttgatttgaa gataatgttt tactaccaac ggtaaaaatc ggtcattccg
    13981 catggtattc acgaaaagtt tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat
    14041 gaactatgaa atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg
    14101 atgttgtgtt ggcgacaatt gatgaacacc gggtcgcaca gctggcaagc ttgagctggc
    14161 cgatcccggt gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac
    14221 tttcgcagtt tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaatg
    14281 ccttgcttaa ggaacaaaac ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc
    14341 aaaccgtctg gcatgaaccg acgggcgtgg caccacacac tttacagatt ggcgataaca
    14401 atcaaattgt ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg
    14461 ccttgggagg acaaggcgca ccgctggtac ctgcgttcca tcatgccttg ctggctcacc
    14521 caaccgagcg acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc
    14581 ctgggcagcc ggttggcggc tacgataccg gtcctggtaa catgctgatg gatgcctgga
    14641 tctggcgtca ggcaggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag
    14701 ttattctccc actgctgcaa aatatgctca gcgacccgta tttctcgcaa cctgcaccga
    14761 aaagcaccgg acgcgaatac tttaactacg gctggctgga gcgccattta cgccattttc
    14821 cgggtgttga tccccgtgat gttcaggcca cactggcaga actcaccgcc gtgaccattt
    14881 ctgaacaagt tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc
    14941 gtaatccgct actcatggcg cgtctggcgg tattactgct aggcacagaa gtcaccacca
    15001 ccgatgccgt tggcattagt ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct
    15061 ggcggacgct ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga
    15121 cggtactggg ggctattttc cccgccaact cgtgatatca gaattaactg aattttttct
    15181 tatcgtgtct gccgttacac ttggaagatt aaggagggta tttgccctcc ggaccaggaa
    15241 agtcttcggg atatgactat gaaaaaactg ttaattatca ttttgccggt ccttctctcc
    15301 ggttgtagcg cctttaatca actggttgag cgaatgcaaa ccgatacgct ggaataccag
    15361 tgtgatgaaa aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt
    15421 tacgataatc aactactgca tctcaaacag ggcttttcag cctctggcgc gcgttactcc
    15481 gacgggatct atgttttctg gtcgaaaggt gaggaagcga ctgtctataa acgcgaccgc
    15541 atcgtcttga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
    15601 gcacaatagc gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt
    15661 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct
    15721 tcccgccgat ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact
    15781 ggcggaccct accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg
    15841 catcgtttta ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag
    15901 ccgtaaagca catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
    15961 ccttgaacgc caggttatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat
    16021 gaaatatttt catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc
    16081 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
    16141 tcaacagggc gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca
    16201 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga
    16261 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
    16321 atcgctggta ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag
    16381 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
    16441 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
    16501 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
    16561 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
    16621 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
    16681 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt
    16741 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
    16801 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
    16861 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
    16921 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
    16981 ctgaacaaac agtacgatgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
    17041 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
    17101 ccgctgatca ccaaagcaga cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
    17161 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat
    17221 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
    17281 ctagaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
    17341 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
    17401 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
    17461 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat
    17521 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
    17581 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
    17641 ggtcgtttta ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
    17701 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
    17761 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
    17821 ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
    17881 ttctaatcac acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
    17941 cgaaattgtg caaggcattg ccattgccgc cattgataaa ttacacacct gtgatgccgt
    18001 attaagtggc tatctgggat cggcggagca gggtgaacat gtcctcggta tcgtccgtca
    18061 ggtgaaagca gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga
    18121 aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtacggcacg gtttgcctgc
    18181 cagcgatatc attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa
    18241 taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt
    18301 gttggttaaa cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt
    18361 caccgccgat gaagcctggc atatcagccg tccactggtg gattttggta tgcgccagcc
    18421 ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac
    18481 gctgcaggag gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa
    18541 agcaatgcag gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga
    18601 acattacttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg
    18661 gccgatggct attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca
    18721 gccatacgtt gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg
    18781 tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag
    18841 atccagtgct catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg
    18901 cgaactgtcg gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta
    18961 tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt
    19021 accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg
    19081 ccttccgtca gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta
    19141 acggaaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc
    19201 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt
    19261 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
    19321 gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa
    19381 ttttacgcta cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc
    19441 agtttaggcg cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc
    19501 aagaatacgc gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca
    19561 gccatcatac ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag
    19621 ctacccataa tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc
    19681 agaccagaga tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg
    19741 acagacacga tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca
    19801 ccagaacaca tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc
    19861 ttgttataga tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc
    19921 tgatactgct caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca
    19981 aagaagttca gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc
    20041 atcaggatga aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct
    20101 ttaccgaaga taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc
    20161 tgattgtgca gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc
    20221 aacaggttac ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag
    20281 cgttggcaga aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac
    20341 gcaacactcc agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag
    20401 ccgatgttga cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc
    20461 tcatagcatg tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca
    20521 atagccgcca tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca
    20581 taaccaatcg ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg
    20641 tcacctaacc agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa
    20701 agggtgattg aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt
    20761 ccttgtaggc cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac
    20821 gccttcggtt gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg
    20881 gacactttta cctctttttt tacatcccat attaacgggg gggctttctc gcgttatgtc
    20941 taaaaaacat acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc
    21001 ttgcactttc agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc
    21061 aaccattccg ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt
    21121 ttcactgcat tgagaggggg atgatggctt tacggagata tgttctgcta tttagcgttt
    21181 ttcctagtga gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata
    21241 tttgttgcgt gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc
    21301 cacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
    21361 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
    21421 attgacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
    21481 tgttacccct tttcttcaga tgtcaactct ctctttgtat tcacaaagat cttcaataat
    21541 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
    21601 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
    21661 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
    21721 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
    21781 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc
    21841 cagtaatata cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt
    21901 tttgatatag gttttcaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
    21961 caccgggtag agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag
    22021 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
    22081 attgttctca cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc
    22141 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt
    22201 ctttcatcaa tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt
    22261 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
    22321 gcccagctac ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg
    22381 cgtagtgaac ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc
    22441 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
    22501 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
    22561 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
    22621 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
    22681 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
    22741 aatagcggac aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga
    22801 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
    22861 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
    22921 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
    22981 tgcgcatcat ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg
    23041 cgcaattcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
    23101 agtaccgtgc cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
    23161 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
    23221 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca
    23281 ttgttatagc gttcggctgg cagcacctga ccaaataacg ccttttgttg cagactggcc
    23341 tgttcagcaa ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc
    23401 gctgcaaata gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
    23461 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
    23521 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
    23581 actaataagc ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta
    23641 actgggtcag tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt
    23701 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
    23761 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg
    23821 ccaccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
    23881 tagatcggat attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat
    23941 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca
    24001 ccaccactgg cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg
    24061 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
    24121 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
    24181 tgtttagcga tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg
    24241 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
    24301 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc
    24361 gacgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
    24421 cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg
    24481 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg
    24541 gcggcttttt tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg
    24601 acctgttctt ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg
    24661 gcaatagcgg cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg
    24721 gcctgttgct gttccagctt gcgtgctttg gc
//
LOCUS       DADLXX010000051        24602 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLXX010000051 DADLXX010000000
VERSION     DADLXX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.051"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..247
                     /locus_tag="JNX27_002036"
     CDS             <1..247
                     /locus_tag="JNX27_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883257.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNX27_002037"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNX27_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7883258.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNX27_002038"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNX27_002038"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7883259.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JNX27_002039"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JNX27_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7883260.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="JNX27_002040"
     CDS             complement(3258..3542)
                     /locus_tag="JNX27_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883261.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNX27_002041"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNX27_002041"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7883262.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="JNX27_002042"
     CDS             complement(4443..5651)
                     /locus_tag="JNX27_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883263.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /locus_tag="JNX27_002043"
     CDS             5735..6271
                     /locus_tag="JNX27_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7883264.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="JNX27_002044"
     CDS             complement(6446..8131)
                     /locus_tag="JNX27_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7883265.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="JNX27_002045"
     CDS             8401..8778
                     /locus_tag="JNX27_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883266.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNX27_002046"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNX27_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7883267.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="JNX27_002047"
     CDS             9225..9512
                     /locus_tag="JNX27_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7883268.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="JNX27_002048"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="JNX27_002048"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY7883269.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="JNX27_002049"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="JNX27_002049"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7883270.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="JNX27_002050"
     CDS             11269..11745
                     /locus_tag="JNX27_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7883271.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="JNX27_002051"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="JNX27_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7883272.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRVKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="JNX27_002052"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="JNX27_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7883273.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="JNX27_002053"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="JNX27_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7883274.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="JNX27_002054"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="JNX27_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7883275.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="JNX27_002055"
     CDS             16302..16790
                     /locus_tag="JNX27_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7883276.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="JNX27_002056"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="JNX27_002056"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7883277.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNX27_002057"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNX27_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7883278.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNX27_002058"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNX27_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7883279.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNX27_002059"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNX27_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7883280.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNX27_002060"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNX27_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7883281.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNX27_002061"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNX27_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7883282.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="JNX27_002062"
     CDS             complement(22242..22757)
                     /locus_tag="JNX27_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7883283.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..>24602
                     /locus_tag="JNX27_002063"
     CDS             23406..>24602
                     /locus_tag="JNX27_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883284.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKI"
BASE COUNT         6171 a         5931 c         6262 g         6238 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgt gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 agacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaacaattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg ttttattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 ta
//
LOCUS       DADLXX010000052        24461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLXX010000052 DADLXX010000000
VERSION     DADLXX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.052"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..488)
                     /locus_tag="JNX27_002064"
     CDS             complement(<1..488)
                     /locus_tag="JNX27_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7883285.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKMKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            761..949
                     /locus_tag="JNX27_002065"
     CDS             761..949
                     /locus_tag="JNX27_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883286.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            961..1671
                     /gene="bcsQ"
                     /locus_tag="JNX27_002066"
     CDS             961..1671
                     /gene="bcsQ"
                     /locus_tag="JNX27_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7883287.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1668..4281
                     /gene="bcsA"
                     /locus_tag="JNX27_002067"
                     /pseudo
     CDS             1668..4281
                     /gene="bcsA"
                     /locus_tag="JNX27_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4292..6631
                     /gene="bcsB"
                     /locus_tag="JNX27_002068"
     CDS             4292..6631
                     /gene="bcsB"
                     /locus_tag="JNX27_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7883288.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCEKPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6638..7744
                     /gene="bcsZ"
                     /locus_tag="JNX27_002069"
     CDS             6638..7744
                     /gene="bcsZ"
                     /locus_tag="JNX27_002069"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7883289.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7803..7979
                     /locus_tag="JNX27_002070"
                     /pseudo
     CDS             7803..7979
                     /locus_tag="JNX27_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8307..10055
                     /gene="bcsC"
                     /locus_tag="JNX27_002071"
                     /pseudo
     CDS             8307..10055
                     /gene="bcsC"
                     /locus_tag="JNX27_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10137..12125
                     /gene="hmsP"
                     /locus_tag="JNX27_002072"
     CDS             10137..12125
                     /gene="hmsP"
                     /locus_tag="JNX27_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7883290.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12309..13595
                     /locus_tag="JNX27_002073"
     CDS             12309..13595
                     /locus_tag="JNX27_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7883291.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13816..15312
                     /locus_tag="JNX27_002074"
     CDS             13816..15312
                     /locus_tag="JNX27_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7883292.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15408..16556)
                     /locus_tag="JNX27_002075"
     CDS             complement(15408..16556)
                     /locus_tag="JNX27_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7883293.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKDADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSVENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16569..17336
                     /gene="pdeH"
                     /locus_tag="JNX27_002076"
     CDS             16569..17336
                     /gene="pdeH"
                     /locus_tag="JNX27_002076"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7883294.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17406..19466
                     /locus_tag="JNX27_002077"
     CDS             17406..19466
                     /locus_tag="JNX27_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7883295.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19648..20970)
                     /locus_tag="JNX27_002078"
     CDS             complement(19648..20970)
                     /locus_tag="JNX27_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7883296.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21381..22394)
                     /gene="yhjD"
                     /locus_tag="JNX27_002079"
     CDS             complement(21381..22394)
                     /gene="yhjD"
                     /locus_tag="JNX27_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7883297.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22443..23414)
                     /locus_tag="JNX27_002080"
     CDS             complement(22443..23414)
                     /locus_tag="JNX27_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7883298.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            23862..>24461
                     /locus_tag="JNX27_002081"
     CDS             23862..>24461
                     /locus_tag="JNX27_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7883299.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5791 a         6165 c         6730 g         5775 t
ORIGIN      
        1 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
       61 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
      121 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
      181 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
      241 aataatttca ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
      301 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
      361 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
      421 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
      481 tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa gaaataaacc
      541 taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa ggtcacaaga
      601 cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa agtcaaaaag
      661 tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg gttagaattg
      721 tatcatgaat ggtacggtta tttcataggg atcaagcaaa atgaataaca atgaaccaga
      781 tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg cgttaaagca
      841 ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac agttggccgc
      901 ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg ggattggtga
      961 atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac catcaccgcc
     1021 gcattagcct ggtcattaca aatgttggga gaaaatgtcc tagtggtcga tgcctgcccg
     1081 gacaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg ctgggccaga
     1141 gcgatgctgg atggccagga ctggcgtgac gctgggttgc gctacacctc gcagctcgat
     1201 ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca ctggcaaacc
     1261 cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg ttaccagtgg
     1321 attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct gagtttgtgc
     1381 gagcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact gcatcagcaa
     1441 gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag tcaggttcag
     1501 gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat gctcattcat
     1561 cgtgatgaag cgatggctga atgcctggcg gctgaagaga tactgacgct ggcgaactgg
     1621 tgcctgttga actactccgg gctgaaaacg ccagtcggga gtgcatcatg agtatcctga
     1681 cccggtggtt gcttatcccg ccggtcaacg cgcggcttat cgggcgttat cgcgattatc
     1741 gtcgtcacgg tgcgtcggct ttcagcgcga cgctcggctg tttctggatg atcctggcct
     1801 ggatttttat tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc
     1861 tgtatacgcc aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc tcattcaaac
     1921 atgctggtta ttgatcggtg catcgcgcaa agaaacgccg aaaccgcgca gcgggcattt
     1981 tcaggtctgc aaaatattcg tggacgttac catcaatgga tgaacgagct gcctgagcgc
     2041 gttagccata aaacacagca tctggatgaa aaaaaagagc tcggtcattt gagtgccggg
     2101 gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc tgattctggc gttaatctgc
     2161 gttactcagc cgtttaaccc gctggcgcag tttatcttcc tgatgctgct gtggggggta
     2221 gcgctgatcg tacggcggat gccggggcgc ttctcggcgc taatgttgat tgtgctgtcg
     2281 ctgaccgttt cttgccgtta tatctggtgg cgttacacct ctacgctgaa ctgggacgat
     2341 ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg aaacgtacgc gtggattgtg
     2401 ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc gtcagccggt gccattgccg
     2461 aaggatatgt cgctgtggcc gtcagtggat atctttgtcc cgacttacaa cgaagatctc
     2521 aatgtggtga aaaataccat ttacgcctcg ctgggtatcg actggccgaa agacaaactg
     2581 aacatctgga tcctcgatga cggcggcagg gaagagtttc gccagtttgc gcaaaacgtg
     2641 ggggtgaaat atctcgcccg caccactcat gaacatgcga aagcgggcaa catcaacaat
     2701 gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg actgcgacca cgtaccaacg
     2761 cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag agaaacagct ggcgatgatg
     2821 cagactccac accacttctt ctcgccggac ccgtttgaac gcaacctggg acgttttcgt
     2881 aaaacgccga acgaaggcac gctgttctat ggtctggtgc aggatggcaa cgatatgtgg
     2941 gacgccactt tcttctgcgg ttcctgtgcg gtgattcgtc gtaagccgct ggatgaaatt
     3001 ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata cttctctgcg tttgcaccgt
     3061 cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg cggggctggc gaccgaaagt
     3121 ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg ggatggtaca aatcttccgt
     3181 ctcgataacc cgctcaccgg taaaggactg aagtttgctc agcggctgtg ctacgtcaac
     3241 gccatgttcc acttcttgtc gggcattcca cggctgatct tcctgactgc gccgctggcg
     3301 ttcctgctgc ttcatgccta catcatctat gcgccagcgt tgatgatcgc cctattcgtg
     3361 ctgccgcata tgatccatgc cagcctgacc aactcgaaga tccagggcaa atatcgccac
     3421 tctttctgga gtgaaatcta cgaaacggtg ctggcgtggt atatcgcacc accgacgctg
     3481 gtggcgctga ttaacccgca caaaggcaaa tttaacgtca ccgccaaagg tggactggtg
     3541 gaagaagagt acgtcgactg ggtgatctcg cggccctaca tcttccttgt tctactcaac
     3601 ctggtgggcg ttgcggtagg catctggcgc tacttctatg gcccgccaac cgagatgctc
     3661 accgtggtcg tcagtatggt gtgggtgttc tacaacctga ttgttcttag cggcgcagtt
     3721 gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc gcgtggagat gacgatgccc
     3781 gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta ccgttcagga tttctccgac
     3841 ggtggtttgg ggatcaagat caacggtcag gcgcagattc tggaagggca gaaagtgaat
     3901 ctgttgctta aacgcggtca gcaggaatac gtcttcccga cccaggtggc gcgcgtgatg
     3961 ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc agcaacatat cgattttgtg
     4021 cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc aggacagcta cccggaagat
     4081 aagccgctgg aaagtctgct ggatattctg aagctcggct tccgtggcta ccgccatctg
     4141 gcggagtttg cgccttcttc ggtgaagggc atattccgtg tgctgacttc tctggtttcc
     4201 tgggttgtat cgtttattcc gcgccgcccg gagcggagcg aaacggcaca accatcggat
     4261 caggctttgg ctcaacaatg atgataacgc gatgaaaaga aaactattct ggatttgtgc
     4321 agtggctatg gggatgagtg cgttcccctc tttcatgacg caggcgacgc cagcaacgca
     4381 accactgatc aatgctgagc cagctgtagc cgcccagacg gaacaaaatc cgcaggtggg
     4441 gcaagtgatg tcaggcgagc agggagctga tgcccccatc gtggcgcaga acggtccttc
     4501 gcgtgatgtg aagctgacct ttgcgcaaat cgcgccgccg ccgggcagca tggtgctacg
     4561 tggtattaac ccgaacggca gcattgagtt tggtatgcgc agcgatgaag tggtgacgaa
     4621 ggcgatgctc aacctcgaat acacgccatc gccatcgtta ctgcctgtcc agtcgcagtt
     4681 aaaggtttat ctcaatgatg agctgatggg cgtgctgcca gtgaccaaag aacagttggg
     4741 taaaaaaacg ctggcgcaaa tgcccattaa tccactgttt attaccgact tcaaccgtgt
     4801 gcggctggag tttgtcggcc attatcagga cgtgtgcgaa aaaccggcca gcaccacgct
     4861 ttggctggat gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa
     4921 gaatgacctg tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac
     4981 cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt
     5041 cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag aacctcccgg tgctctataa
     5101 ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct
     5161 gcgcgatcat ccggcggtaa aagccccggt gattgagatg attaaccatc cgcagaatcc
     5221 ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa
     5281 aggtatcgct cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa
     5341 accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt
     5401 cacatttggc gaactgaaaa cctatgaaga acagttacaa tccagcggcc ttgagccagc
     5461 agcgattaac gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat
     5521 tgatatggat attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga
     5581 tatcagcctg aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa
     5641 ccgtctgctg ctgcgtattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc
     5701 tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa
     5761 cccgatgcca ggcggttcgg tagataactg tattaccttc cagccggtgc agaatcatgt
     5821 ggtgattggt gacgattcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc
     5881 ggatctacgc gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca
     5941 aaccatcacc gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgaatac
     6001 tgttggtttt atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga
     6061 tggcagcacc attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa
     6121 actgaaagac gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac
     6181 accgatgcgc cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga
     6241 aacccagtca acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc
     6301 gtataacgac cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat
     6361 gcttaacgat gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt
     6421 gatccgcgag tacggtatca acagcctacg tgttggcgac gtttattacg taggtcatct
     6481 gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct
     6541 ggcggctatc agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag
     6601 tcgtcgtcgt cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat
     6661 cgtgacgatg ctgttgttgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg
     6721 ggagcagttt aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc
     6781 gcgcaaaatc accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa
     6841 cgaccgtgca gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc
     6901 tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt
     6961 gctggacagc aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc
     7021 ggggcgtttg tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat
     7081 cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt
     7141 gggttttgct gaggataaca gctggcgttt taaccccagc tacatgccgc cgacgctggc
     7201 gcagtatttc acccgctttg gcgcgccgtg gaccacgctg cgcgaaacca atcaacattt
     7261 attgctggaa accgccccga aaggcttttc gccagactgg gtgcgctatg agaaagacaa
     7321 aggctggcag ctaaaagccg aaaaaacatt gatcagcagc tacgacgcta tccgcgttta
     7381 catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa gcgcggatgc tcaaccggtt
     7441 taaaccgatg gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc
     7501 tacggggaaa gcgcagggta aaggaccggt cggtttttct gccgccatgc tgcctttttt
     7561 acaaaaccgc gatgcgcagg cagttcagcg ccagcgcgtg gccgataact ttcccggcag
     7621 cgatacctat tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt
     7681 ccgcttctcg acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca
     7741 ctaaacatat tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca
     7801 accatcagca gaccctgcgg atggacagcg gcaacactaa tgccgtgcgc gggctggcga
     7861 atatttaccg ccagcagtcg ccagaaaaag ctgaagcgtt tatcgcctcg ctctctgcca
     7921 gccagcggcg cagcattgat gatatcgaac gcagcctgca aaacgaccgt ctggcacagc
     7981 aggcagaggt actggaaaac cagggcaaat gggcgcaggc ggcagcactt cagcggcaac
     8041 gactggcgct ggaccccggc agcgtatgga ttacttaccg actttcgcag gatctctggc
     8101 aggccggaca acgcagccag gccgatacgt taatgcgcaa tctggcgcag cagaagccga
     8161 acaacccgga gcaggtttac gcttacgggc tgtacctctc aggtcacaac caggacagag
     8221 cggcgctggc gcatatcaac agcctgccgc gcgcgcagta gaacagcaat attcaggagc
     8281 tggttaatcg actgcaaagc gatcaggtgc tggaaaccgc taatcgcctg cgagaaagcg
     8341 gcaaagaggc tgaagcggaa gcgatgctgc gtcagcaacc accttccacg cgtattgacc
     8401 tcacgctggc tgactgggcg taacaacgac gtgattacac cgccgcccgc actgcatatc
     8461 agaatgtcct gacgcgggag ccaactaacg ccgatgccat tcttggtctg acggaagtgg
     8521 atattgctgc cggtgacaaa gcggcggcac gtagccagct ggcgaaactg cctgccaccg
     8581 ataacacctc gctgaacact cagcggcgcg tggcgctggc acaggcgcag cttggagata
     8641 ccgcagcggc gcagcagacg tttaataagt tgatcccgca ggcaaaatct cagccaccgt
     8701 cgatggaaag cgcgatggtg ctgcgcgacg gtgcgaagtt tgaagcgcag gcgggcgatc
     8761 caaagcaggc gctggaaacc tacaaagacg ccatggtcgc atccggtgtg actacgacgc
     8821 gtccgcagga taacgacacc tttacccgac tgacccgtaa cgacgagaaa gatgactggc
     8881 tgaaacgcgg cgtgcgcagc gatgcggcgg acctctatcg ccagcaggat cttaacgtca
     8941 cccttgagca cgattactgg ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc
     9001 acactaccat gttgcaggtg gatgcgccgt attctgacgg gcggatgttc tttcgcagtg
     9061 atttcgtcaa tatgaacgtc ggcagtttct ccactaatgc cgatggcaaa tgggatgaca
     9121 actggggcac ctgtacatta caggactgta gcggcaaccg cagccagtcg gattccggtg
     9181 ccagcgtggc ggtcggctgg cgaaatgacg tttggagctg ggatatcggt accacgccga
     9241 tgggcttcaa cgtggtggat gtggtcggcg gcatcagtta cagcgatgat atcgggccgc
     9301 tgggttacac cgttaacgcc caccgtcggc ccatctccag ttctttgctg gcctttggtg
     9361 ggcaaaaaga ctccccgagc aataccggga aaaaatgggg tggcgtacgt gccgacggtg
     9421 tggggctaag tctgagctac gataaaggtg aagtaaacgg cgtctgggca tcgcttagtg
     9481 gcgaccagtt aaccggtaaa aatgtcgaag ataactggcg cgtgcgctgg atgacgggct
     9541 attactataa ggtcattaac cagaacaatc gccgcgtcac aatcggcctg aacaacatga
     9601 tctggcatta cgacaaagat ctgagtggct actcactcgg tcagggcggt tactacagtc
     9661 cgcaggaata cctgtcgttt gccataccgg tgatgtggcg ggagcgcacg gaaaactggt
     9721 cgtgggagct gggggcgtct ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt
     9781 atccgctgat gaatctgatc ccgaccgact ggcaggaaga agctgcgcgg caatccaacg
     9841 atggcggcag cagtcagggc ttcggctaca cggcgcgggc attacttgaa cgacgtgtta
     9901 cttccaactg gtttgttggc acggcaattg atatccagca ggcgaaagat tacgcaccca
     9961 gccatttcct gctctacgta cgttattccg ccgccggatg gcagggtgac atggatttac
    10021 cgccgcagcc gctgatacct tacgccgact ggtaagtttt cagatagcgc ctctcttaat
    10081 gccgctgcga tcgggtatac tcgggcggca atctgggatt tccgggggga gacaatttgc
    10141 gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt
    10201 tcgtttttat tttttgcacc gttttgctgt tccatctggt ccagcagaat cgctataaca
    10261 cggctacgca actggaaagc attgctcgct ctgtccgcga acccttatct tcagctattt
    10321 tgaaaggcga tattcccgaa gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg
    10381 tcagccgtgc cgatgtagtg ctgcctaacc agttccaggc gctgcgtaaa agttttattc
    10441 cagagcgtcc ggtgccggta atggttactc gcctgtttga gctaccggtt caaatctcgc
    10501 tgggcgtcta ctcgctcgaa cgtccggcaa atccgcagcc aatagcctat ctggtgctac
    10561 aggcggattc cttccgtatg tataagttcg tgatgagcac tctctcaacg ttagtgacca
    10621 tttacttact tttgtcgctt atcctgaccg tcgccatcag ctggtgcatt aaccgcctga
    10681 ttttgcatcc gttacgcaat attgctcgcg aacttaacgc catcccagcc caggagcttg
    10741 ttggtcacca actggcatta ccgcgtctgc atcaggacga tgaaatcggt atgttggtgc
    10801 gcagttacaa cctcaaccag caattgctgc agcgccatta tgaagaacag aacgaaaatg
    10861 cgatgcgctt cccggtgtcg gatttgccga acaaagcctt gctgatggag atgctggagc
    10921 aggttgtcgc gcgtaaacaa accaccgcgc tgatgatcat cacctgtgaa accctgcgtg
    10981 atactgcggg cgtgctgaaa gaggcgcaac gagaaattct gctgctgacg ctggtggaaa
    11041 aactcaaatc ggtactgtcg ccacgtatga tcctcgcgca gattagcggt tatgactttg
    11101 ctgtcattgc caacggtgta caggaaccgt ggcacgcaat cactttgggt cagcaagtac
    11161 tcactatcat gagcgagcgc ctgccgattg aacgtattca actccgtccg cactgtagca
    11221 ttggcgtggc gatgttctac ggcgatctca ccgccgaaca gctttacagt cgcgctattt
    11281 ctgcggcatt taccgctcgc cataaaggca agaatcagat tcagttcttt gatccgcagc
    11341 agatggaagc agcccagaag cggttgacgg aagagagcga tatccttaat gcactggaaa
    11401 atcatcagtt tgcaatttgg ttacagccac aggtcgagat gaccagcggt aaactggtca
    11461 gtgcggaagt gttactgcgt atccagcaac cggatggcag ttgggacctg ccggatggct
    11521 taatcgatcg cattgagtgc tgtgggctga tggttaccgt cggtcactgg gtgctggaag
    11581 agtcctgtcg attgcttgca gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa
    11641 acctctctgc gctgcaactg atgcacccga atatggtggc ggatatgctg gaactgttaa
    11701 cccgctatcg cattcagccg ggaacactga ttctggaagt gacagaaagc cgacgtattg
    11761 acgaccctca tgctgcggtg gcaatcctcc gtccgctgcg caatgccgga gttcgggtgg
    11821 cgctggatga tttcggtatg ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt
    11881 tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga aggcttgccg gaagatagca
    11941 gcatgattgc tgcaattatc atgctggcgc agagcctgaa cttacaaatg attgccgaag
    12001 gcgtggagac tgaagcacaa cgagactggc tggcaaaagc gggcgttggt attgcccagg
    12061 gcttcctttt tgctcgccca ctccctattg aaatcttcga agagagttac ctggaagaaa
    12121 agtagctacc ccaaactgat tacaaaactt taaaaagtgc tggtttgtgc gagccagctc
    12181 aaacttttta acctttttgt ttcaattatg atccaggtac atttctgtga tgttgtctgg
    12241 gtgttatttt aaggccgcag gtacccccat aaccttacaa gacctgtggt tttactaaag
    12301 gacaccctat gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg acagcgatag
    12361 ccattggtat tctccttggc catttctatc ctgaaatagg cgagcaaatg aaaccgcttg
    12421 gcgacggctt cgttaagctc attaagatga tcatcgctcc tgtcatcttt tgtaccgtcg
    12481 taacgggcat tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc gcagtcgcac
    12541 tgctttactt tgaaattgtc agtaccatcg cgctgattat tggtcttatc atcgttaacg
    12601 tcgtgcagcc tggtgccgga atgaacgtcg atccggcaac gcttgatgcg aaagcggtag
    12661 cggtttacgc cgatcaggcg aaagaccagg gcattgtcgc cttcattatg gatgtcatcc
    12721 cggcgagcgt cattggcgca tttgccagcg gtaacattct gcaggtgctg ctgtttgccg
    12781 tactgtttgg ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt tttaacgtga
    12841 tcgaaagttt ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt ctggcaccta
    12901 ttggtgcgtt cggggcaatg gcgtttacca tcggtaaata cggcgtcggc acactggtgc
    12961 aactggggca gctgattatc tgtttctaca ttacctgtat cctgtttgtg gtgctggtat
    13021 tgggttcaat cgctaaagcg actggtttca gtatcttcaa atttatccgc tacatccgtg
    13081 aagaactgct gattgtactg gggacttcat cttccgagtc ggcgctgccg cgtatgctcg
    13141 acaagatgga gaaactcggc tgccgtaaat cggtggtggg gctggtcatc ccgacaggct
    13201 actcgtttaa ccttgatggc acatcgatat acctgacaat ggcggcggtg tttatcgccc
    13261 aggccactaa cagtcagatg gatatcgtcc accaaatcac gctgttaatc gtgttgctgc
    13321 tttcttctaa aggggcggca ggggtaacgg gtagtggctt tatcgtgctg gcggcgacgc
    13381 tttctgcggt gggccatttg ccggtagcgg gtctggcgct gatcctcggt atcgaccgct
    13441 ttatgtcaga agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg accattgtcg
    13501 ttgctaagtg ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg aataatcgtg
    13561 cgccggatgg caaaacgcac gaattatcct cttaatccca ccacttatgc ccgcactccc
    13621 ttccccgagt gcgggtgttt gcgcataatt gacccctgtg cctgaactta ccacctgggt
    13681 gagttcaggt ttatttcact tcttccgtga cattttcatg ttcttgcggt ctaacacgaa
    13741 gtgtttttaa cgtcatttag actgaccgac aacggacagt ttttttatta ccaggatagt
    13801 tgatcagggg ttcacatgca gggcataaaa attcgacttt tagcgggcgg tttgctgatg
    13861 atggccactg ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag
    13921 gggacgcttt ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat
    13981 cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc
    14041 ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca
    14101 caggcgcgtt cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt
    14161 gtctcttatg acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg
    14221 aaagaagcgc tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc
    14281 atcaaccacg cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag
    14341 ggctggtggc gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg
    14401 ctgaaacaac ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg
    14461 gatgcaatga cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc
    14521 aacaaaacgt ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt
    14581 tctccgctgc gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta
    14641 tctatcatgt gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac
    14701 tggcgtgcgg acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc
    14761 agtgacagca aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag
    14821 tgtgccatca acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg
    14881 cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg
    14941 gcgcaaaaga aactggagct gcagaaactg tttgccgcct atgcacgggc tgataccgat
    15001 attctgatgg gccagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag
    15061 cagtatcaga aactgcggca ggatttcctt aacagcctga ctgtggagat gttaaatcag
    15121 gatctgcgtc agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag
    15181 ccggaattta acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatctacc
    15241 gccgctgcga ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca
    15301 cctgcacagt aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag
    15361 ctggatagag gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc
    15421 gcctcacgcg ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg
    15481 cgtttcgccg cgttttccac actgccgccc gtcaaacgta ccgccagata accggcgctg
    15541 aaagagtcgc cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga
    15601 acatcaacta acgcttcacc agcaatggac accaggcaag aacccgcccc gcgtttcacc
    15661 accacttctt tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga
    15721 ccccacagcg cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt
    15781 tgctggtaca cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg
    15841 tcgaaaatca cttttccgcc tttggcgcgg cattcgcgca gcagggaaag cagcttttcg
    15901 cggctggtcg ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc
    15961 gccagctctt cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt ggcggcggct
    16021 tcgttacgcc agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg
    16081 cccggaagac ggttttccat ccgttgggtc agggaagtat caacgttctc gccgtgccag
    16141 gcgtccagca tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt
    16201 aatgccgcag gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg
    16261 aaaccgcgct taacgtccgc gtctttctcg gaaagctcaa tcatgcattc gccaatcacg
    16321 gcaatctttt tagacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct
    16381 gtggtggttt gctggtcaac catattaaaa cagtgttcca ttattttttt gagccagaac
    16441 gtattcctga aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt
    16501 ctgcccttac gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga
    16561 catctttgat gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga
    16621 gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct
    16681 atcaaacatg cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga
    16741 acccttcgca acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga
    16801 tggaggttgt gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc
    16861 acggcctgct ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac
    16921 caaaaatcct gcgccagatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata
    16981 tccgtctgcc gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg
    17041 atgattttgg taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca
    17101 tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc cgcacactct
    17161 tttctcagct tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag
    17221 aaacaccgga agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt
    17281 ttctttcacg cccggcaccg atggaaacgc tgaatacggc ggttctggcg ctataagctg
    17341 cctcattttc cgccgggctg gactatcttt aggactggca caggaagaat atgaggaagc
    17401 gaacgatgag caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga
    17461 ttgtcgtggc gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca
    17521 accagaaagt ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg
    17581 tgtgggagcg gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac
    17641 atgcagaaga catcattctt ggtaatccac cggatattcc cgaagtcacg atggtgcatt
    17701 tgccgcgagt agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc
    17761 cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg cctctctgaa aagaacaata
    17821 actggacgtt taatcttgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat
    17881 ggtcgtttcg gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag
    17941 taagcaaagc ggatctggaa atttttgttg atccgttagg caagccgctg ccattcagcg
    18001 aagttactgg atcgaaaggt aaagcggata aagaaaaggt gggcgattac atttttggcc
    18061 tgaaggcgca gggacgttat aacggtgaac cgctcacggg tacgggaaaa ataggcggta
    18121 tgctggcgct gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta
    18181 atacccgtgt tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt
    18241 tacggcttaa attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc
    18301 tgcccgatac cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa
    18361 aatcgtcggt ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg
    18421 gttctctggt ctacaccacc ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc
    18481 ggcaattgcg gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag
    18541 aaaagtcgaa acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag
    18601 tgctgcctta tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct
    18661 tcaaagggcg gcgcattgag catggcagta gcctgccgat tagcgatctt tctactcata
    18721 tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca
    18781 gcattgcggc gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata
    18841 ttcaggctcg tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga
    18901 cgctggggga aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc
    18961 ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca
    19021 acctgatgga gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg
    19081 acgatgaggt gcgggtgaac tgcgcggcgg cgaatctgca tattgccaac ggcgtggcgc
    19141 gcccgcagat ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa
    19201 gttttgcttc ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta
    19261 tcacactgcg ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga
    19321 aagccggacc gctgattgcc cgtggtgctg ttgctgcggc actggcaacg ctggtaacac
    19381 cggcggcggc gttactggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga
    19441 cgatattgtc gcagatgaag aagtgatgat aattgccgga tgtctgacat ccggcaatta
    19501 aacagattta gatttttggc aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct
    19561 tatccggcct acaaatcctt gtgaattcaa tatattgtat ggaaattcag tcctgataag
    19621 cgtagcgcat caggcttttc acacttatta caacgactga tgtcgcgtct catgggtcag
    19681 cagcagggcg attaacgtca agccagccat cgccgccaga tataacccca ccgcacctaa
    19741 cccgtagtta gcctgcaacc aagctgcgat atacggcgca acggaagccc cgagaatcga
    19801 cgatacgttg taagagaacg atgctccggt gtaacgcact tctgtcggaa acagctctgg
    19861 taacagcgca cccatcggcc cgaaggtcag ccccatcaga cttaacccca gcagcaggaa
    19921 ggcaaaaacc agaatcgggt tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa
    19981 caggatgatc agcgtggtga tgatcaccat gcttttacgg cgaccaaagg catcagccag
    20041 taatccggcg actggcacca tcacgccaaa accaataact gccatcatca gcatccacaa
    20101 cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactga aggtcataga
    20161 gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg tacccagtac
    20221 ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct gttttttcgc
    20281 tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat acaggccgat
    20341 aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca taaactgctc
    20401 atcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg
    20461 tgcgcctagc tgtgggaagg agccatacag tgcacgtttg cgcggtgggg cgttttcagt
    20521 cgccagcagc gccgcgccgc cccattcacc gcctaagccc agaccctgac caaatcgagc
    20581 cagcgccagc agcagtgggg cgaaaatacc aatcgtggca tagcccggca gcagaccaat
    20641 caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac
    20701 gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga aggcgatggc
    20761 gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga agaagatatg
    20821 cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact caatggccgt
    20881 gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt attcttgttc
    20941 gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg ctaacgaata
    21001 gccatattac gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa
    21061 aaaccaggca aaaaggggat aatgtttagg ccagtgattt ttcttctatg aaatgcttca
    21121 cataatttta taaatagtgg ataaaactgg tttcaggtta aataaaagtt accaactgga
    21181 tttatatgct ggggttatga gttggagtga aattgtttgc tcccaaatag atggctgaag
    21241 aggctctggg gaagagtttc aagaggttat gtgcgcatta tagccggatg cgatgctggc
    21301 gcatcttatc cggcctacgg ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt
    21361 tacgccgcat ccggcctatt ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta
    21421 tattcggcgg tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa
    21481 aatgccatca gccccagcac ggaaccaaat gctgcgccag agggggattt catcagcgat
    21541 ggcagggtgt aggtcatcac gattttaatc acttcaaaac caatagcagc cagaaatgtt
    21601 ccgcgaatca gggctttttt gcgcggacgg tggcgcggca accgccagaa gatccagaag
    21661 aaaagcagat agttggcgaa gatggaaatc gccaggccaa tcaatcgcca cgtcggtttc
    21721 agccactcaa tgctgttcag gtgcagggcg ctaataatca tttgctgcgc cgaaccggca
    21781 accgaggtga tcgaaagcgt cacaatcagc gcaatcaaca aaccaatcag tgaaataaaa
    21841 tcacgcagat attttaccca gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg
    21901 cgcgactggg cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc ggaataaagt
    21961 gccaccgcca ggccgacaag ccctacagtc gtacgctgct gaacggcggt gttgatggtg
    22021 tttttcaacg tggcggctag tgtcggatcg ctgatgtttt gcagaatttt gtcgaagata
    22081 tcctgtagca acatcggatg ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc
    22141 aaaatcggga tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc
    22201 agccgatcgt taaagcgttc tgtcgcgcga ataaggtgcg caataaccgg ttggcgttgg
    22261 actttttcgg cagtggtggt gacggtttcc agcgcctggc taactttgcc gcctttctcg
    22321 ctattatcca actgcttaat cggctcgtgc tccagatcct gggtgggacg tttgatctcg
    22381 ttttcctgcg tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa aagtatagtc
    22441 ggtcagtcca ggtgctcttt cattacgcca gccagccact gcataaacag gtttacacgc
    22501 cgggaaagct cccgacgctg cgggtaaacc agcgaaaggg agagcggctc ggcacggtag
    22561 cctggcaaca cttcaataag ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg
    22621 atctgaataa tccccagacc ggcaagaccg gcggcgagat agttttcgct actgtttacc
    22681 gtcaacatgc cgccagactt aaaccactgg acgccattaa cgctggcaac ctcaaaacct
    22741 aacggatgta cacccaggtg cggtgtataa cgcactattg cgtgtgaagt cagatcatcg
    22801 gggctttgcg gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt gaccatggtt
    22861 agtttgccga ggggacgggc gataacgcca tcttccggta acgcgccagt gcgtatcacg
    22921 caatcaaaac catcatgaag aatatctacc ggacggttat ggctactcag ttccagctca
    22981 attcccggat gctgatagag aaattccgac agacgcggca gtaacaggtt tttcgcgatt
    23041 cctgacggga tgtcgatgcg taatttaccg ctgatactgg tggcatcctg ttgaaacaga
    23101 ccgtccagtt cgctgagatt actcaacaca tcttttgccc gttgataata ggtcatgcct
    23161 tctggcgtga gtttgacccg tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt
    23221 tccagtgcct gtatctggcg cgaaacactt ccctttggca aagcaaagaa atccgccgcg
    23281 cgggaaaaac tttccagctc cgcgactttg atgaacaact gcattgcgtg aattttatcc
    23341 atttctttga ccgattgttg ttcacagcga aacagtaacg cgcatatcgc attgtttatt
    23401 gttttttcta acatctaatg ccgggtttca caatgttgtc atgacgaatg tggcgatttc
    23461 tcagcaggaa aagggcggaa tagctttatg agaaaggtga atccccttaa acagagcgta
    23521 tttttcagtg attcccttta aagtgaatag aggacgtatt ggctatttgt gcttattttt
    23581 gtctcattgc catttgttat tttggcttgt agtcgcggaa tttcattttt aattgcatgc
    23641 gtactaacca ccaatataag gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt
    23701 aatttcaggc gtttagctgt aactatttat atcggtaact attttctacg cgtcatctct
    23761 gtagaatggc caccgccaaa tggcgaattc ttattttttc tcgcgggtaa tacccttcca
    23821 ctacttaagt ctttttaacg tattcaggtg gagattcagt tatgcaaata gtcatgtttg
    23881 acaggcagtc aatatttatt catggaatga aaatcagttt acagcagcgt attccaggag
    23941 tgagtattca gggggtcagt caggcagacg agttatggca aaagctggaa agttaccctg
    24001 aagccttagt tatgctcgat ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa
    24061 aaaccgtggt gcagtttcct gaggttaagg tgttaatcac ggcgacggat tgcaataaac
    24121 ggtggttaca ggaagttatc cattttaatg tgctggccat tgtgcctcgc gattcaaccg
    24181 tcgagacatt tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt ctgcctggag
    24241 actggcggac gacgccggaa aaggacatta aagatctcaa atcgttaagc gcccgccagc
    24301 gggagatttt aaccatgtta gcagcgggcg aatcaaataa ggagatcggc agagcgctga
    24361 atatcagtac cggaacggta aaagcgcatc tggaatccct gtatcgccgt ctggaagtga
    24421 aaaatcgcac ccaggcggca atgatgttaa atatctcctc c
//
LOCUS       DADLXX010000053        24248 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLXX010000053 DADLXX010000000
VERSION     DADLXX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.053"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..647
                     /gene="coaBC"
                     /locus_tag="JNX27_002082"
     CDS             <1..647
                     /gene="coaBC"
                     /locus_tag="JNX27_002082"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7883300.1"
                     /translation="ITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSG
                     PVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQA
                     TQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNVEEYARQKRIRKNLDLICA
                     NDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRYDEKNRR"
     gene            625..1083
                     /gene="dut"
                     /locus_tag="JNX27_002083"
     CDS             625..1083
                     /gene="dut"
                     /locus_tag="JNX27_002083"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7883301.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            1190..1786
                     /gene="slmA"
                     /locus_tag="JNX27_002084"
     CDS             1190..1786
                     /gene="slmA"
                     /locus_tag="JNX27_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7883302.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(1823..2464)
                     /gene="pyrE"
                     /locus_tag="JNX27_002085"
     CDS             complement(1823..2464)
                     /gene="pyrE"
                     /locus_tag="JNX27_002085"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7883303.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(2530..3246)
                     /gene="rph"
                     /locus_tag="JNX27_002086"
     CDS             complement(2530..3246)
                     /gene="rph"
                     /locus_tag="JNX27_002086"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7883304.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            3373..4236
                     /locus_tag="JNX27_002087"
     CDS             3373..4236
                     /locus_tag="JNX27_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7883305.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4457..5281
                     /gene="dinD"
                     /locus_tag="JNX27_002088"
     CDS             4457..5281
                     /gene="dinD"
                     /locus_tag="JNX27_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7883306.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            5572..6189
                     /locus_tag="JNX27_002089"
     CDS             5572..6189
                     /locus_tag="JNX27_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7883307.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(6186..7868)
                     /gene="ligB"
                     /locus_tag="JNX27_002090"
     CDS             complement(6186..7868)
                     /gene="ligB"
                     /locus_tag="JNX27_002090"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7883308.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            8126..8749
                     /gene="gmk"
                     /locus_tag="JNX27_002091"
     CDS             8126..8749
                     /gene="gmk"
                     /locus_tag="JNX27_002091"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7883309.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8804..9079
                     /gene="rpoZ"
                     /locus_tag="JNX27_002092"
     CDS             8804..9079
                     /gene="rpoZ"
                     /locus_tag="JNX27_002092"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7883310.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            9098..11206
                     /gene="spoT"
                     /locus_tag="JNX27_002093"
     CDS             9098..11206
                     /gene="spoT"
                     /locus_tag="JNX27_002093"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7883311.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            11213..11902
                     /gene="trmH"
                     /locus_tag="JNX27_002094"
     CDS             11213..11902
                     /gene="trmH"
                     /locus_tag="JNX27_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7883312.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            11908..13989
                     /gene="recG"
                     /locus_tag="JNX27_002095"
     CDS             11908..13989
                     /gene="recG"
                     /locus_tag="JNX27_002095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7883313.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(14023..15228)
                     /gene="gltS"
                     /locus_tag="JNX27_002096"
     CDS             complement(14023..15228)
                     /gene="gltS"
                     /locus_tag="JNX27_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7883314.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLRLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            15508..16899
                     /gene="xanP"
                     /locus_tag="JNX27_002097"
     CDS             15508..16899
                     /gene="xanP"
                     /locus_tag="JNX27_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7883315.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17020..18729
                     /locus_tag="JNX27_002098"
     CDS             17020..18729
                     /locus_tag="JNX27_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7883316.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNREWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(18782..21100)
                     /gene="yicI"
                     /locus_tag="JNX27_002099"
     CDS             complement(18782..21100)
                     /gene="yicI"
                     /locus_tag="JNX27_002099"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY7883317.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(21110..22493)
                     /locus_tag="JNX27_002100"
                     /pseudo
     CDS             complement(21110..22493)
                     /locus_tag="JNX27_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            22786..22880
                     /locus_tag="JNX27_002101"
     tRNA            22786..22880
                     /locus_tag="JNX27_002101"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22820..22822,aa:Sec,seq:tca)
     gene            23152..24248
                     /locus_tag="JNX27_002102"
                     /pseudo
     CDS             23152..24248
                     /locus_tag="JNX27_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         6051 a         6483 c         6117 g         5597 t
ORIGIN      
        1 tgattaccgc cggtccgacg cgtgaaccgc tcgatccggt gcgttatatc tctaatcaca
       61 gctccggcaa gatgggtttt gctatcgccg ccgctgctgc ccgtcgtggc gcgaacgtca
      121 cgctggtatc aggtccagtt tcactaccga cgccaccgtt tgttaaacgt gttgatgtga
      181 tgaccgcgct ggaaatggaa gccgccgtga atgcttctgt acagcagcaa aatattttta
      241 tcggttgcgc cgccgtggcg gattatcgcg cagctaccgt ggccccagaa aaaatcaaaa
      301 agcaggccac gcagggtgat gaattaacaa taaaaatggt taaaaacccc gatatcgtcg
      361 caggcgttgc cgcactaaaa gaccatcgac cctacgtcgt tggatttgcc gccgaaacaa
      421 ataatgtgga agaatacgcc cggcaaaaac gtatccgtaa aaaccttgat ctgatctgcg
      481 cgaacgatgt ttcccagcca actcaaggat ttaacagcga caacaacgca ttacaccttt
      541 tctggcagga cggagataaa gtcttaccgc ttgagcgcaa agagctcctt ggccaattat
      601 tactcgacga gatcgtgacc cgttatgatg aaaaaaatcg acgttaagat tctggacccg
      661 cgcgttggga aggaatttcc gctcccgact tatgccacct ctggctctgc cggacttgac
      721 ctgcgtgcct gtctcgacga cgccgtagaa ctggctccgg gtgacactac gctggttccg
      781 accgggctgg cgattcatat tgccgatcct tcactggcgg caatgatgct gccgcgctcc
      841 ggattgggac ataagcacgg tatcgtgctt ggtaacctgg taggattgat cgattccgac
      901 tatcagggcc agttgatgat ttccgtgtgg aaccgtggtc aggacagctt caccattcaa
      961 cctggcgaac gcatcgccca gatgattttt gttccggtag tacaggctga atttaatctg
     1021 gtggaagatt tcgacgccac cgaccgcggt gaaggcggct ttggtcactc tggtcgtcag
     1081 taacacatac gcatccgaat aacgtcataa catagccgca aacatttcgt ttgcggtcat
     1141 agcgtgggtg ccgcctggca agtgcttatt ttcaggggta ttttgtaaca tggcagaaaa
     1201 acaaactgcg aaaaggaacc gtcgcgagga aatacttcag tctctggcgc tgatgctgga
     1261 atccagcgat ggaagccaac gtatcacgac ggcaaaactg gccgcctctg tcggcgtttc
     1321 cgaagcggca ctgtatcgcc acttccccag taagacccgc atgttcgata gcctgattga
     1381 gtttatcgaa gatagcctga ttactcgcat caacctgatt ctgaaagatg agaaagacac
     1441 cacagcgcgc ctgcgtctga ttgtgttgct gcttctcggt tttggtgagc gtaatcctgg
     1501 cctgacccgc atcctcactg gtcatgcgct aatgtttgaa caggatcgcc tgcaagggcg
     1561 catcaaccag ctgttcgagc gtattgaagc gcagctgcgc caggtattgc gtgaaaagag
     1621 aatgcgtgag ggtgaaggtt acaccaccga tgaaaccctg ctggcaagcc agatcctggc
     1681 cttctgtgaa ggtatgctgt cacgttttgt ccgcagcgaa tttaaatacc gcccgacgga
     1741 tgattttgac gcccgctggc cgctaattgc cgcgcagttg cagtaatatg acgccggatg
     1801 acttttcatc cggcgagttt ctttaaacgc caaactcttc gcgataggcc ttaaccgccg
     1861 ccagatgttc cgccatttcc ggcttctctt ccagataggc aatcagatct ttcagggtga
     1921 tgatagagat cactttgcag ttgtaatcac gctcaacttc ctgaatcgcc gaaatctcgc
     1981 cgcgcccgcg ttcctgacga tcgagcgaaa tcaacacgcc agcaagcgtc gcgccattgg
     2041 cctgaataat ctccatcgac tcgcgaatcg ccgttccggc ggtgatcaca tcatctacca
     2101 gcattacgcg tccttgtaac gcgctaccaa ccagattgcc gccttcaccg tggtcttttg
     2161 cttctttgcg gttaaagcag tacggcaggt ccaggtcgtg atgctccgcc agtgccacag
     2221 cggttgtggt ggcaatcggg atccctttgt aagcagggcc aaacagcaga tcgaactcaa
     2281 tgccggaatc caccaacgct tcagcgtaaa aacggcctaa cagtgccaga tcgcgcccgg
     2341 tattaaacag cccggcgttg aagaaatagg ggcttttgcg cccggatttc agcgtaaact
     2401 cgccaaactt taacacctgc ttgctaagcg caaattcaat aaactggcgc tgatatggtt
     2461 tcatgccttc gctcctcatc ttacttttct acagacaaaa aaaaggcgac tcatcagtcg
     2521 ccttaaaaat cagtttgcca gcgccgcctt ctgcgtcgct acaatggatt cgattccccc
     2581 tcgggccagc gccaacaagg tgagtagctc ttcatgggtg aacggctcgc cttctgccgt
     2641 cccctgcact tcaatgatgc gcccgtcttc ggtcatcact acgttcatgt cggtctctgc
     2701 ggctgagtct tcaacgtatt ccagatcgca aattgcttca ccgtttacaa taccgacaga
     2761 aactgcggct accatccctt tcatcgggtt ggttttcagc ttgccgtttt ccaccagctt
     2821 ctgtagcgca tctgccagcg ccacgcaggc acccgtaatc gacgcggtac gagtgccacc
     2881 atcagcctga agcacgtcgc agtccagcgt aatggtgaac tcacccagcg ctttcaaatc
     2941 tactgccgcg cgaagagcac gggcgatcag acgctggatt tccattgtgc gtccaccctg
     3001 cttacctttc gccgcttcac gagcgttacg ggtgtgggta gaacgtggca gcatgccgta
     3061 ctctgcggtg atccagccct ggccctgacc tttcaggaag cgcggcacgc cttcttcaat
     3121 agaggcggta cacaatactt tggtatcgcc aaattcgacc agcaccgagc cttctgcatg
     3181 ttttgtatag ttacgagtca gggtaacggg acgcacctga ttattgctac ggcctgctgg
     3241 acgcatattg aaatctccgg cttgaaacaa atgtggctgc gcattatacg gacttccggc
     3301 ggttattcct atcctgacaa ggcatcgatg gctataatcc ttccacctct ccttttataa
     3361 acaggaacgt ctatgatccg cagtatgacc gcctacgccc ggcgtgaaat caagggtgaa
     3421 tgggggagcg caacttggga aatgcgctcg gtaaaccagc gttatctgga aacttacttt
     3481 cgtctgccgg agcagttccg tagccttgaa cctgtcgttc gcgagcgtat tcgttctcgc
     3541 ctgacgcgcg gtaaagtgga atgtaccctg cgctatgagc cagatgttag cgcacaaggt
     3601 gagctgatcc tcaatgaaaa actggctaaa cagctggtaa ctgccgcgaa ctgggtaaaa
     3661 atgcagagtg acgaagggga aatcaacccg gttgatattc tacgctggcc gggcgtgatg
     3721 gcagcccagg agcaggatct cgacgcgatt gccgctgaaa ttctcgcggc gttggatggt
     3781 acgctggacg actttatcgt cgcgcgcgaa accgaaggtc aggcactgaa agcattgatc
     3841 gagcagcgtc tggaaggcgt caccgccgaa gtggtcaaag tccgcgccca tatgccggaa
     3901 attctgcaat ggcagcgtga gcgtctggtc gcgaagctgg aagatgctca ggttcagttg
     3961 gaaaacaacc gtctggagca ggagttggtt ctgctggcac aacgaattga cgttgccgaa
     4021 gagctggacc gcctcgaagc gcatgttaaa gagacgtaca acattctgaa gaaaaaagaa
     4081 gcggttggtc gtcgtctgga ctttatgatg caggagttca accgtgagtc gaacactctt
     4141 gcgtcgaaat ctatcaatgc cgaagtgaca aactccgcca tcgagctgaa agtgttgatt
     4201 gagcagatgc gcgagcagat tcagaatatt gagtaactcg taacaccaat tctgactttt
     4261 cagccttcac aaatgccgcc cactcaaaca gagcggcatt tttcttcccc gcaacttcaa
     4321 ttctgttttg cgtgcctgct ccagattttg cgatgttttt ttgcccagca cactgagaac
     4381 gtgagatact cacaactgta tataaataca gttacagatt tactttcttt gcaatcgata
     4441 tcacatggag tgggcaatga acgaacatca tcaacctttt gaagagataa aactgattaa
     4501 tgcaaacgga gcagaacaat ggtcagcaag acaacttggg aaactactgg gttattcaga
     4561 gtatcgtcac tttatacctg tattaacgcg cgccaaagag gcctgtgaga acagtggtca
     4621 cacaattgat gaccatttcg aggagatcct cgatatggtc aaaattggct caaatgccaa
     4681 aagagcatta aaagacatcg tactctcccg ctatgcctgt tacctggtag tacaaaacgg
     4741 cgaccccgcg aaaccggtca ttgcggcagg gcagacttat tttactatcc agacccgacg
     4801 gcaggagctt gctgacgatg aagcatttaa gcaacttcgt gaagacgaaa agcgtctgtt
     4861 tctgagaaat gagttgaaag aacataacaa acaattggtt gaggcagcac agcaagctgg
     4921 ggtagcgaca gctactgatt tcgccatatt tcagaatcat ggttaccagg ggctgtatgg
     4981 tggattagat cagaaagcca tccaccaact gaaggggctg aaaaagagtc agaagatcct
     5041 ggatcatatg ggttcaacag aactggcggc taatctcttt cgagctaccc aaacagaaga
     5101 aaaactcaag cgggatagcg tgaattcaaa acagcaagct aataccacac actttgacgt
     5161 gggtagcaag gtgaggcaaa ccattcagga acttggcgga accatgcctg aggagttgcc
     5221 gaccccgcag gtcagcatca agcagttgga aaacagtgta aaaattacag agaagaaata
     5281 gcagaaacga aaaactcttt ttgttgattg agacacccga tgcgtaagcc aaggtccagg
     5341 tgcagtgaac acaatggcta aacattgcac ctttcttttc ccccctcagt tttaacctat
     5401 tttttcttat gcattttctc agacaagaag tcagaagaat gcatctacgc tacagaaaat
     5461 agcgatttca cataactaaa agttatcaat ttcctctcac cttaaaaaat atcaatcgtg
     5521 acaatgcgca caaatcgcta ccctgccaga cagattttta gggagagaac catgctgtta
     5581 cacattttgt atttggttgg cattactgcc gaagccatga cgggggcgct ggcggccggg
     5641 cgacggcgca tggatacatt tggcgtaatt attattgcaa ccgccaccgc aattggcgga
     5701 gggtcagtgc gcgatattct gctgggccac tatccgctcg gctgggtcaa acacccggaa
     5761 tatgtgatta tcgtcgctac cgccgcagta ctcaccacta tcgttgctcc tgtaatgcct
     5821 tacctgcgca aagtgtttct ggtgctcgat gcgctcggac tggtggtctt ttctatcatc
     5881 ggcgcacagg ttgcactgga tatggggcac ggcccaatta ttgccgttgt cgcggcggta
     5941 accaccggtg tatttggcgg cgttttgcgc gatatgttct gtaaacgcat tccgctggtc
     6001 tttcaaaaag agttgtatgc cggtgtctca tttgcctccg ccgtgctgta catcgcgctg
     6061 caacactatg tttctaacca tgatgtggtg attatctcca ccctggtatt cggctttttt
     6121 gctcgtttac tggcgctacg tcttaagctg ggattaccgg tcttttacta cagccacgaa
     6181 ggtcactaag gttcaaaacc tgtgatctgc tgggcagcca gccaactgcc cagcttcttg
     6241 atttgcgcat tttccttcca ttcaataacc tgtctggcgc gcccagatcc agtccccggc
     6301 agctgctgcc agaactgctc cgtgctaaat aaaagttgcg accaggatcg ttcatcactg
     6361 gcattaagcg ccgcccgggt tagcggtatt cccattgcca tcacccagcg agtaaaaggc
     6421 tggttacgag ccagattaaa ctgatgccat aactgcgcac ttttactttt cgcgatcccc
     6481 ggcgtgttct gtaattgctc tggcgttaat aaaagccagg aaaagatatg ctcaaagcga
     6541 tgagtctgat gcagcgcgcg ccaaccggcc tcaccaatgc catccagccc cagaacctgt
     6601 tttgacccca gccagactaa gcgtgaaatg aactgttcct gacaaacatc agaagcaaag
     6661 tagcaggtca acgagttaaa gcggttttct ggtggtgtcg gttttgtacg ttctgcaccg
     6721 cgccagacca catcatcaat gcgaggaata ccctgaccgg caaggctgac gaggatctga
     6781 tcaccaggcg caatatccca ctcctgccag cgcctgacgg aaccaatatt cacccgctgg
     6841 acttttttat catccagcat gacaggtgcg agtgacgcaa ccaccgatat tttaccgctc
     6901 ttacccaccg caaactgaat cgccttcact tcggcaacct gagctacagg ttgatatttc
     6961 caggccacca gccactccgc ctggcccggt agccaatgac gggattcggg ttccttagcc
     7021 cctcgcacaa ccacgccatc ggtgacgaag ggtaatttcg ctttccacca ctcattgcgt
     7081 acgcgcgcaa cctcatcagc atttttaacc gcacgggtat acctctgcgt tagagtaaaa
     7141 cctgcggtag ccagctcctt taaacgatcg gtcattaact gcggtccatc cggccatgcc
     7201 cagacaaaaa cagccagtga gtttagcgtg tcgctattcc cctggcgcat catcaggcca
     7261 gcaacttttg cgcgggcatt tattcccccc atttgttgtt ggatatgccc ctcgcgctgg
     7321 agaaatattt ccccctgaag cgtactgttg gctaaaggcc cgctaacggt ttgcggcaca
     7381 gcggaaatta agctaacttt ctgcgtccag tcctcgcctt tcaggccatt accgcgactg
     7441 attgctttgt tcagtttccc gtcccgataa accagggtta ccgcaacgcc atcaactttt
     7501 ggctgcaccc aaagatcgct acgttctcgc atccacagac ttaatgcgtt tttatccgcc
     7561 attttgcgta cacccgtatg ggcaaccgga tgcataaccg caccatttaa aggcggcatc
     7621 atcacgtcgc ggggctcgct cacaaagcag cgttgccact gcgtaagacg ggcgcttaac
     7681 tgatcgtaaa caccgtcttc cacctcactt tttccttcct tccagtagtc atcgtcccac
     7741 tgttttattt gctgttgcag gcgggaaatt tcttcctgtg ctctggctgg cgaccaggcc
     7801 ggacagaccg cccacacaga tgattgccag cacaagatac ttattaatat cgccatccat
     7861 actttcatct tcacctccgc tgtagtcagg ctggcagata tacaacgtgg tgaaggcaaa
     7921 gccgagtggc aaaaacggag tctgcgagga cgcttcctga gaatcgtctt tattgcagta
     7981 aatcacaggc aaatgcggaa acagctacgc aaaacgcaac aactttgcgc aaaaagtgtg
     8041 agcaagggct acgtcacatg gccgcgccgt gtataataag ctcgtatgta ggctttattt
     8101 cgctaatcac atacgaaaga tactcatggc tcaaggcacg ctttatattg tttctgcccc
     8161 cagtggcgcg ggtaaatcca gcctgattca ggctttatta aaaacccaac cgttgtatga
     8221 cacccaggtt tctgtttcac acaccacacg ccaaccgcgt cctggtgaag tccacggtga
     8281 acattatttc tttgttaatc atgatgaatt taaagaaatg attagcagag atgcgttcct
     8341 cgaacacgca gaagtttttg gtaattacta tggcacttcg cgtgaggcca ttgagcaagt
     8401 actggcgacc ggtgtcgatg tttttctcga tatcgactgg cagggcgcgc agcaaattcg
     8461 ccagaagatg ccgcacgcgc ggagtatctt tattttaccg ccgtccaaaa ttgaactgga
     8521 ccgccgtcta cgcggtcgcg gtcaggacag cgaagaggtc attgcaaagc gtatggcgca
     8581 agctgttgca gaaatgagcc attacgccga atatgattat ctgattgtga atgatgactt
     8641 cgataccgcg ttgaccgatt tgaagaccat tattcgcgcc gaacgtctgc gcatgagccg
     8701 ccaaaagcag cgtcatgacg ctttaatcag caaattgttg gcagactgaa cctgatttca
     8761 gtatcatgcc cagtcatttc ttcacctgtg gagcttttta agtatggcac gcgtaactgt
     8821 tcaggacgct gtagagaaaa ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg
     8881 cgctcgtcag atgcaggtag gcggaaagga tccgctggta ccggaagaaa acgataaaac
     8941 cactgtaatc gcgctgcgcg aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt
     9001 tcgcgaacgc caggaacagc aagagcagga agccgctgaa ttacaagccg ttaccgctat
     9061 tgctgaaggt cgtcgttaat cacaaagcgg gtcgcccttg tatctgtttg aaagcctgaa
     9121 tcaactgatt caaacctacc tgccggaaga ccaaatcaag cgtctgcggc aggcgtatct
     9181 cgttgcacgt gatgctcacg aggggcaaac acgttcaagc ggtgaaccct atatcacgca
     9241 cccggtagcg gttgcctgca ttctggccga gatgaaactc gactatgaaa cgctgatggc
     9301 ggcgctgctg catgacgtga ttgaagatac tcccgccacc taccaggata tggaacagct
     9361 ttttggtaaa agcgtcgccg agctggtaga gggggtgtcg aaacttgata aactcaagtt
     9421 ccgcgataag aaagaggcgc aggccgaaaa ctttcgcaag atgattatgg cgatggtgca
     9481 ggatatccgc gtcatcctca tcaaacttgc cgaccgtacc cacaacatgc gcacgctggg
     9541 ctcacttcgc ccggacaaac gtcgccgcat cgcccgtgaa actctcgaaa tttacagccc
     9601 gctggcgcac cgtttaggta tccaccacat taaaaccgaa ctcgaagagc tgggttttga
     9661 ggcgctgtat cccaaccgtt atcgcgtaat caaagaagtg gtgaaagccg cgcgcggcaa
     9721 ccgtaaagag atgatccaaa aaatcctctc ggaaatcgaa gggcgtttgc aggaagcggg
     9781 aattccctgc cgcgtcagtg gtcgcgagaa gcatctttat tcgatttact gcaaaatggt
     9841 gctcaaagag cagcgttttc actcaatcat ggacatctac gctttccgcg tgatcgtcaa
     9901 tgattccgac acctgttatc gcgtgctcgg gcaaatgcat agcctgtaca agccgcgtcc
     9961 gggtcgcgtg aaagactaca tcgccattcc aaaagcgaac ggctatcagt ctttgcacac
    10021 ctcgatgatc ggcccgcacg gtgtgccggt tgaggtccag atccgtaccg aagatatgga
    10081 tcagatggcg gagatgggtg ttgccgcgca ctgggcttat aaagagcacg gcgaaaccag
    10141 caccaccgcg caaatccgcg cccagcgctg gatgcaaagc ctgctggagc tgcaacagag
    10201 cgccggtagt tcgtttgaat ttatcgagag cgttaaatcc gatctcttcc cggatgagat
    10261 ttacgttttc acaccggaag ggcgaattgt cgagctgcct gccggtgcaa cgcccgtcga
    10321 cttcgcttat gcagtgcata ccgatatcgg tcatgcctgc gtgggcgcac gcgttgaccg
    10381 ccagccttac ccgctgtcgc agccgcttac cagcggtcaa accgttgaaa tcattaccgc
    10441 accgggcgct cgcccgaatg ccgcgtggct gaactttgtc gttagctcaa aagcgcgcgc
    10501 caaaattcgt cagttgctga aaaacctcaa gcgtgatgat tctgtaagcc tgggccgtcg
    10561 tctgctcaac catgctttgg gtggtagccg taagctgaat gaaatcccgc aggaaaatat
    10621 tcagcgcgag ctggatcgca tgaagctggc aacgcttgac gatctgctgg cagaaatcgg
    10681 acttggtaac gcaatgagcg tggtggtcgc gaaaaatctg caacatgggg acgcctccat
    10741 tccaccggca acccaaagcc acggacatct gcccattaaa ggtgccgatg gcgtgctgat
    10801 cacctttgcg aaatgctgcc gccctattcc tggcgacccg attatcgccc acgtcagccc
    10861 cggtaaaggt ctggtgatcc accatgaatc ctgccgtaat atccgtggct accagaaaga
    10921 gccagagaag tttatggctg tggaatggga taaagagacg gcgcaggagt tcatcaccga
    10981 aatcaaggtg gagatgttca atcatcaggg cgcgctggca aacctgacgg cggcaattaa
    11041 caccacgact tcgaatattc aaagtttgaa tacggaagag aaagatggtc gcgtctacag
    11101 cgcctttatt cgtctgaccg ctcgtgaccg tgtgcatctg gcgaatatca tgcgcaaaat
    11161 ccgcgtgatg ccagacgtga ttaaagtcac ccgaaaccga aattaatgtt ttatgaaccc
    11221 aacacgttat gcacgcatct gcgaaatgct cgccaggcgg cagcctgatc tgaccgtctg
    11281 catggagcag gtccacaaac ctcataacgt ttctgcgatt attcgtaccg cagatgccgt
    11341 tggcgtacat gaagttcacg ccgtctggcc tggtagccgc atgcgcacca tggcttcggc
    11401 agcggcgggt agtaacagct gggtacaggt gaaaacacac cgcaccattg gcgatgccgt
    11461 cgctcatctc aaaggccagg gcatgcagat tctggcaacc catctttctg ataatgctgt
    11521 cgatttccgc gaaattgatt acactcgccc gacctgcatt ttgatgggac aggagaaaac
    11581 gggcatcacg caggaagcat tggccctggc ggatcaggac atcatcattc cgatgatcgg
    11641 catggtgcag tcgctgaatg tttccgttgc ctcagccctc attctttacg aagcccagcg
    11701 tcagcggcaa aatgcaggca tgtacctgcg tgaaaacagc atgttgccgg aagcagagca
    11761 acaacgcctg ttgtttgaag gcggctatcc ggtgctggcg aaagtcgcaa aacgcaaagg
    11821 cctgccttat ccccacgtca atcagcaagg cgagatcgaa gctgatgccg actggtgggc
    11881 tactatgcag gctgcagggt aagtgccatg aaaggtcgcc tgttagatgc tgtcccactc
    11941 agttccctaa cgggcgttgg cgcagcactt agtaacaaac tggcgaaaat caacctgcat
    12001 accgtgcagg atctactctt acaccttccc ctgcgctacg aagatcgcac ccatctctac
    12061 cccatcggag aactactgcc gggcgtttat gccacggtgg aaggcgaagt gctgaactgc
    12121 aatatctcct tcggcggtcg gcggatgatg acctgccaga tcagcgacgg ttccggcatc
    12181 ctcaccatgc gctttttcaa tttcagcgcg gcaatgaaaa atagcctggc gacgggccgc
    12241 cgtgtactgg cttatggcga agcaaagcgc ggtaaatatg gtgcggagat gatccacccg
    12301 gaataccgcg tgcagggcga tctcagcacg ccagaattac aggaaacgct cacgccggtt
    12361 tatccaacaa cggaaggcgt aaagcaggcc acgctgcgta aattaaccga ccaggcgctg
    12421 gatctgctcg acacctgcgc cattgaagaa ctcctgccgc cggaactgtc acaaggaatg
    12481 atgacgctac cggaagcgtt gcgcactttg caccgcccgc caccgacgct acagcttagc
    12541 gatctggaaa ccgggcagca tccggcgcaa cgtcgtctga ttctggaaga actgctggcg
    12601 cacaacctca gcatgttagc cttacgtgcc ggagcacagc gttttcatgc ccagccgctg
    12661 agcgccaatg acacgctgaa aaataaactc ctcgccgcct taccgttcaa gccaacgggc
    12721 gcacaggcac gcgtagtggc ggagatcgag catgatatgg cgctggatgt gccgatgatg
    12781 cgcctggtac agggcgatgt aggttccggt aaaacgctgg tcgccgccct cgccgcgctg
    12841 cgtgcgattg cccacggcaa acaggtggca ctgatggcac caaccgaatt actcgccgag
    12901 cagcacgcca ataacttccg caactggttt gcaccgctcg gtatcgaagt gggctggctc
    12961 gccggtaagc agaaaggcaa agcgcgactg tctcagcagg aagccatcgc cagcggtcag
    13021 gtgcagatga ttgtcggtac acacgccatc ttccaggaac aggtgcagtt taacggcctg
    13081 gcgctggtga ttatcgacga acagcatcgt tttggcgtgc atcagcgtct ggcattgtgg
    13141 gagaaaggcc agcagcaggg cttccatccg catcagttga tcatgaccgc cacgccgatc
    13201 ccccgcacgc tggcaatgac ggcgtatgcc gatctcgata cctcggtgat agatgagctg
    13261 ccgccaggcc gcacgccagt gactacggtc gctattcctg atacccgccg taccgacatc
    13321 attgaccgcg tgcgccacgc ctgcataact gaaggtcgtc aggcgtactg ggtttgtacg
    13381 ttgattgaag agtcagagtt actggaagcg caggcggcgg aagctacctg ggaagagttg
    13441 aaactggcgc taccagaatt gaatgttggc ctggtacacg ggcggatgaa accagccgag
    13501 aaacaggcgg tgatggcgtc gtttaaacaa ggtgagctac atctgctggt tgccactacc
    13561 gttattgaag tcggtgttga tgtgcctaac gccagcctga tgattatcga aaaccctgag
    13621 cgtctgggtc tggcgcagtt acaccagctg cgtgggcgcg taggtcgtgg cgcggtggct
    13681 tctcactgcg tgctgctcta caaaacgccg ctttctaaaa cggcgcaaat tcgcctgcaa
    13741 gtgctgcgcg acagtaacga cggttttgtg attgcgcaaa aagatctgga gattcgcggc
    13801 cctggcgaat tgttaggcac gcgtcagacg ggtaatgctg aatttaaagt ggcggattta
    13861 ctgcgcgatc aggcgatgat cccggaagtt cagcgcctgg cgcgacatat tcacgaacgt
    13921 tacccacaac aggcaaaagc cttgatagaa cgctggatgc cggagacaga acgttactcg
    13981 aatgcgtaaa aggcggcggt gagaagaccg ccgcttcatc ggttaaccgg caaaaatcgg
    14041 caacattaaa tacagcttaa tcaccagcgc attgacgata tcgataaaga acgcaccgac
    14101 catcggcacc accaaaaacg ccatgtgcga cgggccaaag cgttcagtga tcgcctgcat
    14161 gttggcgatt gccgttggcg ttgcaccgag gccaaaacca cagtgacccg cagccagcac
    14221 tgccgcatcg tagtttttgc ccatcatgcg ccaggtaacg aagatggcat acaacgccat
    14281 gaagatggtc tgtaccacca gaatcgccag catcggcagt gccagcgaag ccagctccca
    14341 cagtctcagc cccatcaacg ccatcgccag gaacaacgac aagcttacgt tacccagcac
    14401 ggataccgca cgctcaaaga cgcggtaaaa gcccattatt gacagaccgt tgctcagaat
    14461 cacgccaaca aacagtacac agacgaaggt cggcagttca aaagcagtgc cagccaaaag
    14521 ttgcgcaaca attttcccca ccgtcaggca gatagcaatc agcgcgatag tttcaatcag
    14581 caccaacgag gtgatcatgc gtcccacatc cggcttttca aacgccgtcg ggacttcctg
    14641 gtcatccgga ataccgttcg gcgtggtgga gtgtttcacc agatagcgcg ccaccggacc
    14701 gccaatcaag ccgcccagca ccagaccgaa cgttgcacag gccatcgcca cttccgtcgc
    14761 attggtgaag ccataacgtt caatgaacaa tttactccac gcagcgcccg taccgtgacc
    14821 gccggaaagc gtaatagaac cggccaacag ccccatcagc ggatcaagcc ctaacaggct
    14881 agccatacca ataccaatgg cattttgcat caccaacaga ccaacaacca caatcaagaa
    14941 gatgccaacc acacgcccac cggcacgcaa actggcaatg ttggcgttca ggccaatggt
    15001 ggcgaagaaa gccagcatta acggatcgcg cagggacata tcaaagttga cttcccagcc
    15061 catgcttttt ttcagtacta gtagcgccag cgccaccaac aaaccacccg caacaggttc
    15121 cggtatggtg tatttcttca aaaaggagac ggaatggacc aacttacgcc cgagcagcaa
    15181 cgtcagcgtt gcggcaacaa gcgttgctaa agtatcgaga tgaaacatag ttactccttt
    15241 gtatccgcat cactctttca tactcgtcat acttcatcgc gcagtggcaa cttcatgaac
    15301 tatgacgggt atcgagttat gctttcctgg gtcaaattct cagctgttaa tgaacaaatg
    15361 gcatggattt taagcagaaa aataccaaaa gttatataaa aaagtgcatt tatctcattt
    15421 aaatatttag atgaggatgt ggcaaacgtt tgcttttctc tttcggtcag ctaaaatgcc
    15481 cgctttgcta ccacgggatt gttttcaatg tctgtttcca ccctcgagtc agaaaatgcg
    15541 caaccggttg cgcagactca aaacagcgaa ctgatttacc gtcttgaaga tcgtccgccg
    15601 cttcctcaaa ccctgtttgc cgcctgtcag catctgctgg cgatgttcgt tgcggtgatc
    15661 acgccagcgc tattaatctg ccaggcgctg ggtttaccgg cacaagacac gcaacacatt
    15721 attagtatgt cgctgtttgc ctccggtgtg gcatcgatta ttcaaattaa ggcctggggt
    15781 ccggttggct ccgggctgtt gtctattcag ggcaccagct tcaactttgt tgccccgctg
    15841 attatgggcg gtaccgcgct gaaaaccggt ggtgctgatg ttcctaccat gatggcggct
    15901 ttgttcggca cgttgatgct ggcaagttgc accgagatgg tgatctcccg cgttctgcat
    15961 ctggcgcgcc gcattattac gccgctggtt tctggcgttg tggtgatgat tatcggcctg
    16021 tcgctaattc aggtggggct gacctccatt ggcggcggtt acgcagccat gagcgataac
    16081 accttcggcg caccgaaaaa tctgctgctg gcaggcgtgg ttttagcctt aattatcctg
    16141 cttaaccgtc aacgtaaccc ttacttacgc gtggcctcac tggtaattgc gatggcggcc
    16201 ggatatgcgc tggcgtggtt tatgggcatg ttgccagaaa gcaacgaacc gatgacgcaa
    16261 gaactgatta tggtgccaac gccgctctat tacggtcttg gcattgaatg gagtctgctg
    16321 ctgccgctga tgctggtctt tatgatcact tcgctggaaa ccattggcga tatcacggcg
    16381 acctctgacg tttccgaaca gccagtgtcc ggtccgctgt acatgaaacg cctgaaaggc
    16441 ggcgtgctgg caaacggcct gaactcgttt gtttcggcgg tgtttaacac cttcccgaac
    16501 tcctgcttcg ggcaaaacaa cggagtgatc cagttgactg gtgttgccag ccgctatgtc
    16561 ggttttgtcg tcgcgctgat gttgatcgtg ctgggtctgt tcccggcagt gagcgatttt
    16621 gtacaacaca ttccagaacc ggttctgggc ggcgcaacgc ttgtaatgtt tggcaccatc
    16681 gccgcctccg gtgtgcgtat cgtttctcgt gagccgctga accgtcgggc gattctgatt
    16741 atcgcgctgt cgctggcggt tggtctgggc gtgtctcagc agccgctgat tttgcagttt
    16801 gcccctgaat ggctgaaaaa cctgctctcc tccgggatcg ccgcgggcgg tattactgcc
    16861 atcgtgctga atctgatttt cccaccagaa aaacagtaat tcactggcgg cggtaagata
    16921 attaccgccg cagtctcacc tctttcacca ttcccgcctt gaggattgcg cgtaaatcgt
    16981 gcataactcc cttatgtgca tttcacggga tggaagacca tgaaatttat tgggaagctg
    17041 cttctctaca ttctcatcgc tctgttagtg gtgatcgctg gcctctattt tcttctgcaa
    17101 acccgctggg gagcagaaca tatcagcgca tgggtttccg agaatagcga ctatcatctg
    17161 gccttcgggg cgatggatca ccgtttttcc gcgccatctc atatcgtgct ggagaatgtc
    17221 acgtttggtc gtgatggtca gcccgcgccc ctggtggcaa aaagtgtcga cattgcgcta
    17281 agcagtcgcc aactgaccga accacgccat gtcgatacca tcctgctgga aaacgggacg
    17341 ctgaatctca ccgaccagac cgcgccgcta ccgttcaaag ccgatcgtct gcaactgcgt
    17401 gatatggcgt ttaatagccc gaatagagaa tggaaactga gcgcgcagcg ggtaaatggc
    17461 ggcgtggttc cgtggtcacc agaagccggt aaagtgctgg gtacgaaggc gcagattcag
    17521 tttagtgccg gatcgctttc gctcaatgat gttcctgcca ccaatgtact gattgaaggc
    17581 agtattgata acgatcgcgt tacgctgact aacctgggtg ccgacatcgc ccgcgggaca
    17641 ttaaccggaa acgcgcagcg taacgccgac ggcagctggc aagtggaaaa tctgcgcatg
    17701 gcggatatcc gtctacaaag cgaaaaatcg ctaaccgact tctttgcgcc attacgctct
    17761 gtcccgtcgt tgcagattgg tcgcttggaa gtcatcgatg ctcgtctgca aggtccggac
    17821 tgggcggtga ccgacctcga tctcagcttg cgcaacatga ccttcagtaa agatgactgg
    17881 cagacacagg aaggcaaact gtcgatgaac gccagcgagt tcatttatgg ttcgctgcat
    17941 ttatttgacc cgattataaa cgcggaattt tccccgcagg gcgtagcgct gcgccagttc
    18001 accagccgct gggaaggggg tatggtcaga acgtcaggga actggctgcg tgacggtaaa
    18061 acgttgatcc ttgatgatgc ggcaattgcc gggctggaat ataccttgcc gaaaaactgg
    18121 caacagttgt ggatggaaac gacacccggt tggttaaaca gcctgcaact gaagagattt
    18181 agcgccagcc gcaatctgat cattgatatc gaccctgact tcccgtggca gctcaccgcg
    18241 ctcgatggtt acggtgccaa cctgacgctg gttaccgatc ataaatgggg cgtctggagt
    18301 ggctcggcga atctgaatgc cgccgccgcg acattcaatc gtgttgatgt tcgtcgcccg
    18361 tcgctggcgc tgaccgccaa cagcagcacg gtgaatatca gcgaactgag tgcatttact
    18421 gaaaaaggca ttctggaagc caccgcaagt gtttcacaaa cgccacaacg tcagacccat
    18481 atcagcctga atggacgcgg tgtgccggtg aatattttgc aacaatgggg atggcctgaa
    18541 ttacagttga ctggcgacgg caatattcag cttaccgcca gtggagatat tcaggccaat
    18601 gccccgctga aacctacggt tagcgggcaa ttgcatgccg tgaacgccgc aaagcagcaa
    18661 gtgactcaaa ccatgaatgc gggcgtcgtt tccagcggtg aagttacatc gacggagccg
    18721 gtgcggtaaa catcaatctc gccgggtacg actcagacgt acccggcatt ccgtcaatag
    18781 attacaacgt aattgtcagc gcattccctt gaggcgtcac caccagcccc tgctcacttt
    18841 cagcctgcga accgccttgc agaccattta ctttcacaac attgcgcaag cacagcgtcc
    18901 agttcttcgc ctcgcccgca ccagtcacag taatcgtgtt gccagtacgt gctgctttta
    18961 aagtaaagat caccgatccg tcagcagcag gcacttcaca gacggcttca tgcccgtctt
    19021 gcagattaaa gaggtggaat gccgtgcctt cgtgccacac gtaatcggga cgttgatcgt
    19081 tgttgcccag cgccagtaga gtgttatcac gcacataaac gggcagactc aggaagccgt
    19141 gctgctgttt atgccagcga ctaccgtcga gttcatcgtt gtgccacagg tgtgtccagc
    19201 gaccttccgg caggtagaac tgcacatcgc ccgcttcagt gaacaccggc gcaaccatca
    19261 cgttgtcgcc taacatgtat tgacggtcaa ggtaatcaca agccggatcg tccgggaact
    19321 ccatcatcat ggcccgcatc atcggcgtac cccgcgcgtt cgcacgcgca gcttcacgat
    19381 acagatacgg catcatgcgg catttcagtt gcgtgaagaa gcgcaccaca tcacaggact
    19441 catcatcgta ggcccacggc acacgataag atttgctacc gtgtaaacgg ctatggctgg
    19501 agagcaaacc aaacgcgcac cagcgtttgt aaacgtgcgc cggagcggta ttttcaaagc
    19561 cgccgatatc gtggctccag aagccaaaac ctgaaaggcc aatagacaaa ccaccgcgca
    19621 ggctttccgc cattgattcg tagttagcgt aacaatcgcc accccagtgt accgggaatt
    19681 tctgcgcacc gacggaggcc gagcgggcaa acaagacagc ttcttcctca ccaacggtgt
    19741 ccttgagcac gttccacacc agttcgttgt agatgtacgc ataatggtta tgcattttct
    19801 gcggatcgga accgtcaaac cactgaacat cagttgggat acgttcgcca aagtcggtct
    19861 taaagcaatc aacgcccatc gcgaccagac ctttcagttt gtcggcgtac catttgcagg
    19921 catccggatt ggtaaagtca taaatcgcca gacctggctg ccatttatcc cactgccata
    19981 gcgaaccgtc cgggcgtttg agtaaatagc ctttctcttg taactcttta aagacggggg
    20041 atttttgacc gatatagggg ttaatccaga cgcagatttt cagccctttc gctttcaggc
    20101 ggcggatcat cccttccggg tcagggaaag tcagcgggtc ccactcaaaa tcgcaccact
    20161 ggaaggcttt catccagaaa cagtcaaagt ggaaaacatg cagcggcaga ttgcgttccg
    20221 ccataccatc gataaagctg tttaccgtcg cttcgtcgta gttggtggta aatgaagtgg
    20281 ttagccacag gccgaaggac cacgggggcg gcagcgccgg acgaccagta aagcgggtat
    20341 aacgatcaag taccgctttc ggcgtcgggc cgtcgataac aaagtattcg agatattcac
    20401 tctcaacgct gaactgcact ttggagactt tctccgatcc cacttcaaaa gagacacact
    20461 ggggatgatt gaccagtacc ccataaccac ggttagtcat gtagaacggg atatttttat
    20521 acgcctgttc agtacttgtg ccgccgtccc ggttccaggt ctctaccgtc tggccattgc
    20581 gcaccagggc agtaaagcgc tctcccagac cgtaaactgt ttcgccaacg ccaagatcaa
    20641 gccgctcaaa catataattg cgttgattat tcgtgtcctg cacgtagcca ttatttttca
    20701 cctgactacc ggtaatacgt tcgccgttgc gcagaaaatc cagtgaccag aactcacctt
    20761 tgctgacacg cgcgcttaag ttgccacttt taaactcagc ataacgttct gtgttttcga
    20821 ttgtgacctt cacgtcctgc aaaatattga gcggataatg aggaccgtta ttcagcgccc
    20881 cctgaaaata ctcaatccgc acaccgacaa taccttcctg tggggagaaa aagcgcagcg
    20941 taaataaagg cgtatcaagc tgccaggtac gttcacgcac atcacggggg gcagcataga
    21001 ccaccatttc attatcctgc tgttcaacct cgaatacctg aagcgggtga atcaaattga
    21061 ggccaggttg aatcaaccag tttccatcgc taattttcat tgccgttcct tagttctgca
    21121 cttcttgaga ggtgaattgt tgctggcaac ggcgtttacc ctgagccagc tgttccataa
    21181 cggttttcag attgtgcgtc gtgagtgagt agtagcgttt agcgataatc gcgctcagca
    21241 aataacagat cgccggaaca atcgtgaata gcgcaataat gatgctaatc gtggcgctgt
    21301 tctgcgcttt ttctgccgca tcatatccgc cataagccag catccagccg ataagagcgc
    21361 cgccgaaggc caaccccagt ttgagcacaa acagcgtgcc agcaaaactg atcccggtca
    21421 ggcgtttacc attgcaccac tcgccgtagt cgacggtatc gaacatcatt acccactgga
    21481 taggtgtcac cagttgatgc aacacaccaa tcacgaagat gaagacaaac atagtgatgc
    21541 tggcctgcat gggaacaaag aacatcgcga ggctaatcac tgccagcagg gcgttcgtcc
    21601 accagaagat agtgacttta catttccagt cggtcagagg ttttgccagt gcggaaccaa
    21661 tcaggttacc cacgcaataa gtggtgagaa aagcgacaaa cacttccggc gtgcccaaaa
    21721 tccatgtgac gtaatacatc atcgccccac cgcgtacgca caccgccagg atattgaaaa
    21781 tggttagtaa accgacaatc cgccactggt cgttttgcca gatatcacgt aaatcttccc
    21841 gcatagacgt tgttgtaggt ggtgcttcaa cgcgttcttt agtggtgaag aaacaaaatg
    21901 ccagcatcat gaatgccacc acggaaagga ccacgatacc gccctggaaa ccgagtggtt
    21961 tattatcacc gccaattaaa ttaaccagtg gcatcatcag aacagtagaa agcatgcctc
    22021 ccgcggtcgc cagcacaaaa cgccaggatt gcagcgagat acgctgagtc gggtcattgg
    22081 tgattacacc acccaatgcg cagtaaggga tattgacgac ggtataaagt aaggtaagtt
    22141 agggtgtaag taattgctgc atagatcatt ttgccgttca tactgagatc tggcgtgcta
    22201 taggccagta cacagacgat cccgaatggc agtgcgccaa acagtaccca cggacgaaat
    22261 ttaccccagc gagagcgcgt tcgatcggcc aacaacccca tgcaaggatc ggaaatcgca
    22321 tccagtgcac gagcgaccaa aaacatggtt ccgacaaatc cggcaggaat gccaaaaata
    22381 tcggtataaa agaacatcat atataacatt acgttatcga aaataatgtg gctggcggcg
    22441 tctcccatgc cataaccaat tttctcttta acggacaaca cttcactctt catctgcttt
    22501 tccttgatgc tataggggtg ctgagaccga taacgcattt tgtaaaccat ccctgtgaga
    22561 gcgaatattc cgatatctgg ttattaattt atgattcttg ttttatgtga tcgtggtagc
    22621 gttaattccg ctcatatatc attgtaaaat atgggtttta tataaattat aatgctttcg
    22681 tgataatacg ctgcgtttat taggcggaaa aaactgatct gggggatgta gaaactcaag
    22741 gaagtagcta taatgcgccc cgcctccatg tagcaatcga ggcgcggaag atcgtcgtct
    22801 ccggtgaggc ggctggactt caaatccagt tggggccgcc agcggtcccg ggcaggttcg
    22861 actcctgtga tcttccgcca gtttacctct tctgaactct cccaatgtca ataaatctct
    22921 tctaatccct tgtatatcag tatttatttg tatcctgagg tcaacctact tcaccccgta
    22981 tcaagtttct tttgggggta ctttaggggg tgttatttcc tattgaaata caagcacccc
    23041 cataatagga aaaatgtgat acataacaac ggcttttaac aatatctttg ttatacaagg
    23101 ggtgtattgg gggtatcagg ttagttactg gcagaaatcc ccctcaggaa catgaattca
    23161 tcaaaagccg gaggatgtca tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa
    23221 agacaaaccc tataagcttg ctgatggcgg cggtatgtat ctactgatta acactaatgg
    23281 ttctaaatac tggcgcatga agtaccgctt tgctggtaaa gaaaagatgc tttctattgg
    23341 cgtatacccc gatgtaacgc tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact
    23401 cctcgctgct ggaggcgatc caggggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat
    23461 gagtctgaaa aatacgttcg aggctattgc tcgcgaatgg caccagttaa aagctgatcg
    23521 gtggtcatta cgctatcgtg atgaaatcat tgataccttt gaaaaagaca tttttcccta
    23581 cattggcaag cgccctattg ccgaaatcaa accaatggag ttactggaag cactgcgtaa
    23641 aatggaaaaa cgtggagctc tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt
    23701 gttccgctat gcaattgtta ctggccgggc agactataac ccagcccccg atcttgccag
    23761 tgctttagct acaccgaaaa aagtacattt tccctttctt actgctaatg aacttcccca
    23821 cttcctcaac gatctggcgg gttataccgg aagtataatc actaagacag ctactcagat
    23881 cattatgtta accggcgtac gaacacaaga actgcgtttt gcacgctggg aagatatcga
    23941 ttttgagaca aaattgtggg agatcccggc agaagtaatg aagatgaagc gacctcatat
    24001 tgtgccgctc tctgagcagg tcattatgct atttaagcag cttgaaccaa tctcaaaaca
    24061 tcatccccta gtctttatcg gtagaaatga tccccgcaaa ccaattagta aggaaagcat
    24121 taaccaagta attgaattac ttggatataa gggaagactc acagggcacg gtttcagaca
    24181 caccatgagt acaattctgc atgaacaagg ctttaattcc gcctggattg aaatgcagtt
    24241 agctcatg
//
LOCUS       DADLXX010000054        24188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLXX010000054 DADLXX010000000
VERSION     DADLXX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.054"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(22..594)
                     /locus_tag="JNX27_002103"
     CDS             complement(22..594)
                     /locus_tag="JNX27_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883318.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JNX27_002104"
                     /pseudo
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JNX27_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNX27_002105"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNX27_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7883319.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNX27_002106"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNX27_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7883320.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JNX27_002107"
     CDS             complement(3556..5337)
                     /locus_tag="JNX27_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7883321.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JNX27_002108"
     CDS             complement(5330..7102)
                     /locus_tag="JNX27_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7883322.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIHEEAVDA"
     gene            complement(7132..7590)
                     /locus_tag="JNX27_002109"
                     /pseudo
     CDS             complement(7132..7590)
                     /locus_tag="JNX27_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNX27_002110"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNX27_002110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7883323.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLSSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="JNX27_002111"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="JNX27_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="JNX27_002112"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="JNX27_002112"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7883324.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /locus_tag="JNX27_002113"
     CDS             complement(11173..11571)
                     /locus_tag="JNX27_002113"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7883325.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="JNX27_002114"
     CDS             complement(11665..12036)
                     /locus_tag="JNX27_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883326.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNX27_002115"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNX27_002115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7883327.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNX27_002116"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNX27_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7883328.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNX27_002117"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNX27_002117"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7883329.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNX27_002118"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNX27_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7883330.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNX27_002119"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNX27_002119"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7883331.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNX27_002120"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNX27_002120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7883332.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNX27_002121"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNX27_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7883333.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="JNX27_002122"
     CDS             21807..22385
                     /locus_tag="JNX27_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7883334.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="JNX27_002123"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="JNX27_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7883335.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5670 a         6659 c         5833 g         6026 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgctag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg aagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcactc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gtgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cagcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ctgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gcgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctaatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcacgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgactgtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DADLXX010000055        23748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLXX010000055 DADLXX010000000
VERSION     DADLXX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.055"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="JNX27_002124"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="JNX27_002124"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7883336.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="JNX27_002125"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="JNX27_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7883337.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="JNX27_002126"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="JNX27_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7883338.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="JNX27_002127"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="JNX27_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7883339.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3880..3924
                     /locus_tag="JNX27_002128"
     CDS             3880..3924
                     /locus_tag="JNX27_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7883340.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="JNX27_002129"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="JNX27_002129"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7883341.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="JNX27_002130"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="JNX27_002130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7883342.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARCHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREVDLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="JNX27_002131"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="JNX27_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7883343.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="JNX27_002132"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="JNX27_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7883344.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="JNX27_002133"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="JNX27_002133"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7883345.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="JNX27_002134"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="JNX27_002134"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7883346.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="JNX27_002135"
     CDS             10418..10882
                     /locus_tag="JNX27_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7883347.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="JNX27_002136"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="JNX27_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13331
                     /locus_tag="JNX27_002137"
     CDS             11895..13331
                     /locus_tag="JNX27_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7883348.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMPMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNX27_002138"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNX27_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7883349.1"
                     /translation="MRYTDSRKLTLETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNX27_002139"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNX27_002139"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7883350.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNX27_002140"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNX27_002140"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7883351.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="JNX27_002141"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="JNX27_002141"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7883352.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNX27_002142"
                     /pseudo
     CDS             complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNX27_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="JNX27_002143"
     CDS             complement(20164..20457)
                     /locus_tag="JNX27_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7883353.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="JNX27_002144"
     CDS             complement(20454..21743)
                     /locus_tag="JNX27_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7883354.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21769..22736)
                     /locus_tag="JNX27_002145"
                     /pseudo
     CDS             complement(21769..22736)
                     /locus_tag="JNX27_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22756..23520)
                     /locus_tag="JNX27_002146"
     CDS             complement(22756..23520)
                     /locus_tag="JNX27_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY7883355.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5856 a         5784 c         6261 g         5847 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgttg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagcgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagttgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtct aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgccgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaagcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaatcaatg cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt ggcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagggg
    22321 ctgttgatct tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg
    22381 tgaccatcga caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca
    22441 cttaaccgtg ctgccagagc tttaccacgt ttcgtcgcgg ccaacagcaa catagcgggg
    22501 tgtttatctt tcagcagggc agcaatactt tcggcgtaat tttcagtgcg ttgcagcgcg
    22561 tcgttttgct caagaacata aatacatttt ggaccataag gcataactgc ctgcgcctgg
    22621 tcggtatttt gtacaatggc atacacctgt tggccccatt gctgagcgcc gccaaacagt
    22681 tcagcataac gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt
    22741 tcttctccgt aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc
    22801 ctccggcgaa tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat
    22861 gccgaccagt tcagcaagtg gcgcgctctg gctccagtca atatcacttg cctgccactg
    22921 atttaccggt tttttacccg cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt
    22981 aatatcggag gtgacgcaga gcacggctgg aacagagagt tcaataactt caacatcatc
    23041 ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt
    23101 aatcaccgga agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc
    23161 gccggaacct tcaccaaaga tcagtaaatc gaagccgatc ttttcaattg ctgccgccag
    23221 cgcttttgcg gtatcgagag gcagtgcatg ttcaagttgc gcatcctgca ccaaatacag
    23281 gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc gagttctgca acaatgagcc
    23341 gccaacggtc agcgcggcta tctcatcgtc atctgttgcg agctggcttg cagcttcaat
    23401 ggcattgaga tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc
    23461 tggagtgaca acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat
    23521 gatttctcct gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa
    23581 atagtgtgcg atagcggtgc ggggggttcg acggagcaag ctgttttcct tgccaacaat
    23641 cttgcaaaaa aaatgcgatt gctaaaacaa aagtgcaatt tgcggcaata tagttattcg
    23701 gtaatgctgc caacttactg atttagtgta tgatggtgat tttaaggt
//
LOCUS       DADLXX010000056        22800 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLXX010000056 DADLXX010000000
VERSION     DADLXX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.056"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(105..566)
                     /locus_tag="JNX27_002147"
                     /pseudo
     CDS             complement(105..566)
                     /locus_tag="JNX27_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
     gene            1171..1584
                     /gene="hns"
                     /locus_tag="JNX27_002148"
     CDS             1171..1584
                     /gene="hns"
                     /locus_tag="JNX27_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7883356.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1728..2636)
                     /gene="galU"
                     /locus_tag="JNX27_002149"
     CDS             complement(1728..2636)
                     /gene="galU"
                     /locus_tag="JNX27_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7883357.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2838..3851)
                     /gene="rssB"
                     /locus_tag="JNX27_002150"
     CDS             complement(2838..3851)
                     /gene="rssB"
                     /locus_tag="JNX27_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7883358.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFHDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3943..4848)
                     /gene="rssA"
                     /locus_tag="JNX27_002151"
     CDS             complement(3943..4848)
                     /gene="rssA"
                     /locus_tag="JNX27_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7883359.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            4961..5419
                     /locus_tag="JNX27_002152"
     CDS             4961..5419
                     /locus_tag="JNX27_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7883360.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5469..6311
                     /gene="purU"
                     /locus_tag="JNX27_002153"
     CDS             5469..6311
                     /gene="purU"
                     /locus_tag="JNX27_002153"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7883361.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6471..6555
                     /locus_tag="JNX27_002154"
     tRNA            6471..6555
                     /locus_tag="JNX27_002154"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6505..6507,aa:Tyr,seq:gta)
     gene            6602..6733
                     /locus_tag="JNX27_002155"
     ncRNA           6602..6733
                     /ncRNA_class="other"
                     /locus_tag="JNX27_002155"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6917..7594)
                     /gene="narI"
                     /locus_tag="JNX27_002156"
     CDS             complement(6917..7594)
                     /gene="narI"
                     /locus_tag="JNX27_002156"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7883362.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7594..8304)
                     /gene="narJ"
                     /locus_tag="JNX27_002157"
     CDS             complement(7594..8304)
                     /gene="narJ"
                     /locus_tag="JNX27_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7883363.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8301..9839)
                     /gene="narH"
                     /locus_tag="JNX27_002158"
     CDS             complement(8301..9839)
                     /gene="narH"
                     /locus_tag="JNX27_002158"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7883364.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9836..13579)
                     /locus_tag="JNX27_002159"
     CDS             complement(9836..13579)
                     /locus_tag="JNX27_002159"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7883365.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSCSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(13972..15363)
                     /gene="narK"
                     /locus_tag="JNX27_002160"
     CDS             complement(13972..15363)
                     /gene="narK"
                     /locus_tag="JNX27_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7883366.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15702..17498
                     /gene="narX"
                     /locus_tag="JNX27_002161"
     CDS             15702..17498
                     /gene="narX"
                     /locus_tag="JNX27_002161"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7883367.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17491..18141
                     /gene="narL"
                     /locus_tag="JNX27_002162"
     CDS             17491..18141
                     /gene="narL"
                     /locus_tag="JNX27_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7883368.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18142..19536)
                     /gene="ychO"
                     /locus_tag="JNX27_002163"
     CDS             complement(18142..19536)
                     /gene="ychO"
                     /locus_tag="JNX27_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7883369.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19721..20074
                     /gene="ychN"
                     /locus_tag="JNX27_002164"
     CDS             19721..20074
                     /gene="ychN"
                     /locus_tag="JNX27_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7883370.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20118..20813)
                     /locus_tag="JNX27_002165"
     CDS             complement(20118..20813)
                     /locus_tag="JNX27_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7883371.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(20971..21201)
                     /gene="chaB"
                     /locus_tag="JNX27_002166"
     CDS             complement(20971..21201)
                     /gene="chaB"
                     /locus_tag="JNX27_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7883372.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSTWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21471..22571
                     /gene="chaA"
                     /locus_tag="JNX27_002167"
     CDS             21471..22571
                     /gene="chaA"
                     /locus_tag="JNX27_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7883373.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5710 a         5962 c         5488 g         5640 t
ORIGIN      
        1 gcactaagac cgcacgaata taacggtggg ttacccccaa acgaatcgga aaatcgatac
       61 tggaaaaact ctaactcggt ggccagtttt tgttgaccac ttcacaccat gcttgcttta
      121 cggccacaaa aacagatggt ttttaattca accagtttgt cggaccatgc cagtaagtat
      181 tggctgccaa taaataattc acctcgaaaa tcggtacgta aaccataaca aagtacgggt
      241 atatcgagtt gatcgacaac ctccgataat tcatatactt gttgtctggt taaaaactgg
      301 cattcatcaa ccagtacgca atgaattgcc tgctgttcat gttccgcacg aatctcatca
      361 aataatgatg aattttggtt aaataatttt gcaggcgatg acaaacctat acgcgaactg
      421 actttcccgg caccaaagcg atcatcaatt tctgccgtat atacgacagt gcgcatgccg
      481 cgttcctggt aattgtatga agattgcaac aatgctgtag acttacccgc attcattgcg
      541 gaatagtaga aatatagctg tgccatcagc cacaggccct caatgatatg aaataaagtt
      601 ggctggagtt tatcataatt cgtcagtttt tcagctcaac agtatgcaca gaagtgggta
      661 tatgcgttct cccttacgaa gccttgcata atccttctga gctatcatta caactgcctc
      721 gcttgttata agcgggtaaa tgactgctgg taactattca caatctttaa cctgttgcgc
      781 aagtaatagc cctctgttga cctccaggag atagtgcaat actaagtcca tgctcttatt
      841 gcgactgttc tacttttcat cattcgctta atagggaatt ctcgtaaaca caactaatac
      901 agaagactga aaggtcgtca gcctacgata atctccccat aaaatgtgac atgaatcagg
      961 aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat agggctatat gccgcgtctt
     1021 ttctggctaa ttttatgaaa agatatttat tggcggcaca aaataaagaa caattttgaa
     1081 ttccttacat tcctggctat tgcacaactg aatttaaggc tctattatta cctcaacaaa
     1141 ccaccccaat ataagtttga gattactaca atgagcgaag cacttaaaat tctgaacaac
     1201 atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga agaaatgctg
     1261 gaaaaattag aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc tgctgctgaa
     1321 gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa tgctgatcgc tgacggtatt
     1381 gacccgaacg aactgctgaa tagccttgct gccgttaaat ctggcaccaa agctaaacgt
     1441 gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa aacctggact
     1501 ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg taaatccctc
     1561 gacgatttcc tgatcaagca ataatctttt gtagattgca cttgcttaaa atcccgccag
     1621 cggcgggatt ttttattgtc cggtttaaga caatttaata agaataagct ataaaaaaac
     1681 ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat acggatgtta cttcttaatg
     1741 cccatctctt cttcaagcca ggctttaaat tccgtgccaa gggtgttatg acgaataccg
     1801 tattcaacga aggcctgcat gtaacctaat ttattaccgc agtcatggct cttccctttc
     1861 atatgatagg cttccaccgt ttctttttcg atcagcatat caattgcgtc ggtgagctga
     1921 atttcatcac cagctcccgg aggggttttt gccagcaacg gccaaatatc cgcgctaagt
     1981 acgtaacgac ccacaatagc gagattagac ggcgcaacat ccgctttcgg tttttctacc
     2041 acaccaacca tcggtacgct ttcacccggc gctaattcaa cgcctttgca atccacaacg
     2101 ccatatgcgg tcacatcagc aaccggttca accatgatct ggctatgacc cgtttcatca
     2161 aagcggcgga tcatctctgc caggttatcc tgtgacaaat cggattcata ttcatccaga
     2221 ataacatcag gcaaaataac agctaccggt tcatcaccca ctaccgggtg agcacacaat
     2281 accgcgtgtc ccaggccttt cgccagaccc tgacgaactt gcataatagt cacgtgcggt
     2341 ggacaaatag actgcacttc atcaagcagt tgacgtttta cacgtttttc cagcattgcc
     2401 tccagttcaa aactggtatc aaagtggttt tcaatagagt ttttagatga gtgtgtaacc
     2461 agcacaattt cagtaatgcc agccgcaata cattcattca cgacgtattg aattaatggc
     2521 ttatcgacaa gtggcagcat ctctttcggg atggctttcg tcgccggcaa catcctggtt
     2581 cctaatcccg caacggggat aacggctttt ttgactttcg tattaatggc agccatttaa
     2641 attctcctgg actgttcgtg ttttgaacgt gttcatattt ctgtatcgca tcccagtata
     2701 tcagcacccc gaaggggcta agttctgaaa aggaggcgag atagcataat taagacaatt
     2761 acttatacga atggcgacga tagtaccacc agccgacatt agcaggtaat gcaaatttag
     2821 cccgcgttat cgtttgctca ttctgcagac aacatcaagc gcagtcgacc accggttccc
     2881 catatttggc attgccaggc atcgcatcgc tggctcaatt gattcaaata agcgttacct
     2941 aaagtgccta acggaacacc attactgatt tgcacctggt gttcgccggt atttaacgtc
     3001 gcattcagac ccgcagaaac cagaatgaga tttttcagtt cgcgatgata atagccaact
     3061 aatagcggaa actgccccgg cagattggcc tgacgaagta aatggtttac ctgcttcaat
     3121 aacgcgccca actctggcaa ccgttgattt tggtgtgcaa gctgttcctg taataatccg
     3181 ttaaacaatg cgcgtaataa caaggcagca agtacgccat tatgtccagc tcgggtgaca
     3241 tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg caatatcaag caccaggccg
     3301 ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc aatgggaaat cacctgctga
     3361 accggcggtt gtagttcctg taataatttc gccgctgcgg cagggttatc aaccattgca
     3421 tcccagtcgt gaaaaagcct ttcctcttcc tcaacgcgcg aattaaacat gctgggatag
     3481 agacaggcaa aaaccatctc gcgcaagcga ttcagatctt taactggttt cagcaaaaca
     3541 tcttcaacgc ccagacgtaa cgctttggca atatctgcca tattttcagt ggcagatatc
     3601 accagaactg gggtctggtc gcctctgtta cgtatatgct ccagcagttt aagcccgttc
     3661 attcgtggca tcgcgatatc acatatcatc aggtctggag tgaaacctcc cagcaactca
     3721 agggcatcca ccccatcagc cgccagtacc gttgtcgctc ccaatgagga aaaccatgaa
     3781 tccagaagcg agcgaaatac ctgctcatct tcaacaatga gaatctgttt tccgaccaat
     3841 ggctgcgtca tgttctctcc cctgaccggc tttactcaat agtggcatgc tattgccacc
     3901 tgcgcctgtc agaattaact taagtgtaga taaaaattct ggtcaaatgt tggtgcgtac
     3961 caacggcaaa agttcgtcca ttttcctttc cactgccagc tgtccggccg caatggcagc
     4021 gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa tcagaatatc
     4081 gggcggatca cctgccatgc ggttcctttt aaggcggttc tccaacacct ggatagacgt
     4141 ggtcataatc tctgttgccg ttggcgctgt cacagcccga cgtgtcgtta tgctgcctaa
     4201 cctttctttc agacgcgcat gccacggtag agaatcacca ttctcgctat tttcttcact
     4261 gacattaaag gagagcagat cttgttgcat caaatgagca tcgtgctgca ggtcgaccgc
     4321 tatcacaata tcagccccca atgcacgcgt aagagaaata ggaattgggt taactactgc
     4381 cccatcaacc agccagtagc cgttatgtgc aacgggtgcc atgagtcctg gaatactgca
     4441 tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac cataattcac gccccgtact
     4501 taaattggtg gcaacagccg caaagcgacg ggaacaattt tcgatctctg tttccggcat
     4561 tatttcgcga tattgattga agacacgctc gccgcgcagt aacccaccgc gctgccagga
     4621 gaggtccatc aggcgtaaaa cgtcccaata actgaaagag gttacccaat cttccagcgc
     4681 agataatcga tcgcatgcat aggccgcgcc caccagcgaa ccaattgaac atcctgcaac
     4741 gatatcaatt tcaataccca ctttttttag cgcattaata acgccaatat gcgaccaacc
     4801 tctcgccgcg ccagatccca gcgccagccc tatttttatc tttctcatta ttcccgcaag
     4861 attcccctgg aatgcaatcg tagccacatc gctactgctc ggttaacata gtgctactca
     4921 ggcggttaat acgccgttat tgtttccagg gagattattg gtgtctcagc tttgtccctg
     4981 tggtagtgct gtcgagtata gcctatgttg ccacccttat gtgtctggtg aaaaggttgc
     5041 acctgatcca gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc aagacgctga
     5101 ttatttaata aagacctggc atccctcttg tggagcagcg gcgttacgtg ccgaattgat
     5161 gaccggattt gcgcatactg aatggttggg attgagcgta tttgaacatt gttggcagga
     5221 cgccgacaat atcggatttg tcagttttgt tgctcgattt accgaaggtg gcaaaacagg
     5281 tgcaataatt gaacgttcgc gatttttgaa agaaaacggt caatggtact atattgacgg
     5341 tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt ggttcaggta aaaaatttaa
     5401 aaagtgctgc ggccaataat ggttgacggt acggtttagc aaacactctc aacaaggttt
     5461 ttccagcaat gcattcactc caacgtaaag ttctgcgtac tatttgtccg gaccaaaaag
     5521 gtctgatcgc acgtattacc aatatttgct acaagcacga gttaaatatc gtacagaaca
     5581 atgaatttgt tgatcaccgt accgggcgct tttttatgcg cacggaactg gaagggattt
     5641 ttaatgattc caccctgctg gcggatctcg atagcgcatt gccagaaggc tccgtgcgtg
     5701 agctgaatcc tgccggtcgt cgccggatag tgattctggt cactaaagaa gcgcattgcc
     5761 ttggcgattt gttgatgaaa gccaattacg gcggtctgga tgtcgaaatc gcggcagtta
     5821 ttggtaacca cgatacttta cgttctctgg ttgagcgttt tgatattccg tttgagctgg
     5881 taagccatga agggttaagc cgcaacgagc acgatcaaaa gatggcggac gccattgatg
     5941 cttatcaacc tgactatgtg gtgctggcga agtatatgcg ggtattaacg ccggaatttg
     6001 tggcacgctt cccgaataaa atcatcaata ttcaccattc attcctgcct gcgtttatcg
     6061 gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa gattattggc gcaaccgctc
     6121 actatgtgaa tgacaatctg gacgaaggcc caatcatcat gcaggacgtt attcatgtcg
     6181 atcataccta cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag aaaaacgtct
     6241 taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt tgtttacggt aatcggacga
     6301 ttattcttta atcgccagca aaaataactg gttaccttta atccgttacg gatgaaaatt
     6361 acgcaaccag ttcatttttc tcaacgtaac actttacagc ggcgcgtcat ttgatatgat
     6421 gcgccccgct tcccgataag ggagctggct agtaaaaagc attaccccgt ggtggggttc
     6481 ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa ggttcgaatc
     6541 cttcccccac caccatcact ttcaaaagcc ccggaattct caaacgaatc tgcaatcaaa
     6601 tattctgccc aagcggggaa ggatgagaag cttcgaccaa ggttcgactt gagcgccagc
     6661 gagagagcgt tgccgcaggc aacgacccga agggcgaagc gcgcagcgct gagtaatcct
     6721 tcccccacca ccatcgcttc cttgaataat caaaacctgt tactccgagc taattttgcc
     6781 cgatggcacc acgtttaccg ggcctacaat atcctcccct gtaggtcgga taagcgaagc
     6841 gcatccgaca actcaccccg caccgtgccc ataaaaaaac cccgccgaag cagggtctat
     6901 gtgaactaaa attcgtttag tgacgagcgc gcaccagctg gtacttacgt gtcagatact
     6961 ccaccggtac gctccagatg tgtaccagac gcgagaacgg gaacagcagg aataacgtca
     7021 tccccagcac caggtgcaga cggaagataa acgccacacc atcgaggtgt tgagaagcgc
     7081 caccgtggaa ggtcaccacc gactgcgccc agccaaccag tttcatcatc tcgctaccgt
     7141 ccatatgctg agcggagaac ggaatggtca gcaggcccag cgcgcactgg ataacgagca
     7201 gcgacaggat caggatatcc gctccggtag tggttgcacg cacgcgtggg ctgaacatac
     7261 gacgtttcag caaaagcacg ccgccaatca gacacagcac gccgctggca ccaccagcaa
     7321 acatcgccat tttctgtttc acttcaatcg gcagccaggc ttcatacatc cagtgcggcg
     7381 tcaacatacc gaagaagtga ccgacaaaaa tccccagaat accgatatgg aacaggttcg
     7441 acgccaggtt catccctttg cgatccagca tctggctgga cgccgcgcgc caggtgtact
     7501 gcccgtagtc ataacgcagc cagctaccaa tcaggaagac cgccccggct atgtacgggt
     7561 agatatcaaa gaagaacata ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt
     7621 cagatattgc ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga ttgctgaatc
     7681 accgcagcct ttgtcagcaa agaatttaac ctgctcttct tcccagacag catccagcgc
     7741 ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc gccactttgt cgctgtcgat
     7801 agcggtattg gccaatttca gcagcagatc aaacatcacg gcataacggc tttcacgctg
     7861 ttgcagacgc gcgctcagca atgccagaat cggcgcgata tctttcaaac cttccacggc
     7921 ttcgctttgc ggcagctgcg acaggtactc cagatacagc ggcagatggt ccggtaattc
     7981 gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca ccatcgcctg
     8041 gccgcggtca cgggattcac cgtgaacatg ttcaaatagc aacagtgacg tggcgcggcc
     8101 acggtcgaac agttcgctgt actggacctg ggcatcgagc ggatccatcg ccgttaaatc
     8161 gcgcaggaaa atgcccagcg catgggcatc ttctttcgac agatttttcg acgcagcaat
     8221 cgcctcaaac atctcttgtt gatgctgcca taaggcagca tccggatatt caaggagacg
     8281 cgatacaatc acgagttcga tcatggatgc ggctccgttt tgctggtcac atcgatggca
     8341 tcgatacgac ggctgttgaa cagattgaat ttggtatctg agccgtggca gccatcacca
     8401 aaggtaaagc cgcagccatt tttctccggg aaggcttccc gtgccagttc acgatgacta
     8461 ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca gataacggta catctcctgt
     8521 gcctgggctt cggtcagacc gacctcttcc agcgcacggg tatcaacttt accgtcaaca
     8581 gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt tcagcgcacg cagtaccggt
     8641 ttggtatcac cggcggtcag cagattcgcc agatactgta ccggaatacg caagctttcg
     8701 acgtctggca gaattccgtt gctgcccagc tcgcccgcgt ctgctgcaga ctgaatcgga
     8761 gacagaggcg gcacgtacca gaccatcggc agtgtgcgat attctggatg cagcggcagc
     8821 gccagtttcc attccatcgc cattttataa accggcgact gctgtgcagc ttcaataacg
     8881 ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg
     8941 aacacgtcca gctgacgctg gtagagatct ttctcgttct cggtgctggc tgcacgttca
     9001 atcgcgtcgg catcgtacag cagcacgcca agataacgga tacgaccgac acaggtttct
     9061 gagcacacgg tcggctgacc cgcttcaata cgcggatagc agaagatgca cttctcagac
     9121 ttaccgctct tccagttgaa gtagattttt ttgtacggac atccggtgat gcacatacgc
     9181 cagccacggc atttatcctg gtcgatcagg acgatgccat cttcttcacg cttgtaaatc
     9241 gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca caggcgcggc
     9301 aaatacatca taaaggtgtt ttcgaactgg ctatacatcg ccttctggat gttgtcgaag
     9361 ttcttgtctt tcgccagttt atcaaactca ccgcccaggt catcttccca gttcggccct
     9421 ttttcgattt tcgccatccg ttccccggta atcagcgaac gcggacgggc aatcggctgc
     9481 gatttgctgc cttccggcgc ggtatgcagg ttctgataat caaaatcgaa cggctcgtaa
     9541 taatcgtcga tccccggcag atgcgggtta gcaaagattt tacccagcag catggctcgg
     9601 ttacccatgc gcggttgcag tttgccattg attttacgga tccagccgcc tttgtatttt
     9661 tcctggtttt cccagtcagt cgggaagccc tggcccggct tggtttccac gttgttgaac
     9721 cacgcgtatt ccacgccttc acggctggtc cagacgtttt tacaggtaac tgaacaggtg
     9781 tggcagccga tgcacttatc gagattcaac accatgccga cttgtgaacg aattttcatt
     9841 ttacgctctc ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg ttcttcatct
     9901 tacgcactac aacaaactca tcgcggttag aacctacggt gccgtagtag ttaaagccgt
     9961 atgccagatg ggcatagccg ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg
    10021 agttatggat accaccacgc tgttgggtaa tttccgaacc aggcaggtta acgatacgtt
    10081 cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg ctggctgaca accgcacggg
    10141 cagtcagtgc accgttgctg ttgaagactt caatccagtc gttatcggcg atacccagat
    10201 ctttggcatc ggcttcactc aaccagacca ccggaccacc gcgacctaaa gtcagcatca
    10261 gcaggttgtc gctgtaggtg gagtggatac cccacttctg gtgcggcgtc aggaagttga
    10321 gcgctttttc cgggttgccg ttggatttct ggcctatcac ttctttcacc gaacgggtgt
    10381 cgatcggcgg acgataaacc agcaggcttt caccgaaatc acgcatccac tggtgatcct
    10441 gatacagttg ctgacgacca gagagcgtac gccatgggat cagctcgtga acgttggtgt
    10501 aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga ccaggtcggg ctggagataa
    10561 ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt ctcgtcttct ttattcagcg
    10621 ccagatgcgt atggtcacga ccggtaaatt cgctcagggc agcccaggct ttcacggcta
    10681 cctgaccgtt ggtttccggt gccagcgtca ggatcatctc tgccgcatca attgcggtgt
    10741 tcagcatcgg ctggcctttt gccggacctt ccgctttggt gtagttgagc ttacgcagca
    10801 gatccatctc gctctgggtg ttccaggcaa tccctttacc gccgttaccg attttctcca
    10861 tcagcgggcc gatagaggta aagcgttcgt aagttgccgg ataatcgcgc tctacgacca
    10921 taatgtgcgg tgcagttttg cctgggatca ggtcgcattc gccttttttc cagtctttca
    10981 catccagcgg ctgcgccagt tcagcggcag agtcgtgctg gataggcagc gtgacgatgt
    11041 cggtttcttt acccagatgg ccgacgcaca cttcggagaa tttcttcgcg atggctttgt
    11101 agatttccca gtcgctttta gcttcccagg ccggatcgac cgccgcagac agcgggtgaa
    11161 taaacggatg catatccgaa gtattcatgt cgtctttttc gtaccaggtc gccgtcggca
    11221 aaatgatgtc ggaatagaga caggtgctcg acagacggaa gtccagcgta accaccagat
    11281 ccagcttgcc ttccagacca ttgtcctgcc agtccacttc ttccggcttc acgccgccct
    11341 gttgccccag atctttaccc tggataccgt gctccgtccc cagcaggtac ttgagcataa
    11401 actcatgacc tttaccggaa gaaccgagca ggttagaacg ccagatgaac aggttgcgcg
    11461 ggtggttttt accgttttct ggttgttctg ccgcaaaacg gatggaaccc tctttcaggg
    11521 atttcaccgt atagtccacc ggattcatcc cggctttttc cgcttcgcca gcgatagtca
    11581 gcgggttagt gcctaactgc ggtgcagacg gcagccagcc catgcgttcc gcacggacgt
    11641 taaagtcgat caagtgtccg gtatagcggg atttgtccgc catcggtgac agcaactctt
    11701 ccgccgtgac ggtttcataa cgccactggc tggagtggtt atagaaataa gaagtgctgt
    11761 tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc cagccggttt
    11821 gcggacgcag tttttcctga cctacatagt gcgcccagcc gcccccgctc tgaccgacac
    11881 agccgcagaa aatcagcatg ttgatcagac cacgatagtt catatcgagg tgataccagt
    11941 ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg cgttttatca gcgttatcgg
    12001 caaattcacg ggcgatgcga ataatctggc tgcaagaaac gccggtaatc tgctcggccc
    12061 aggccggggt ataagctttc acatcgtcat agctggttgc acagttaacg tcgttcaggc
    12121 cacgttccag accgtagttt gccagcgtca gatcataaac ggtggtcacc agggcggtgc
    12181 tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg ttttccagtt
    12241 ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct acctcagcga
    12301 tctcatcctg gctacccagc aggctcagtt gcagctcggt ttcttcgcca gttttgccgt
    12361 cgcgctgctc aagattccat ttgcccttct cgccccagcg gaagccaata gaaccgttcg
    12421 gcgcaaccat ttcgccattg gtattaaagg cgacagtttt ccattccgga ttgttttcct
    12481 ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg acctgcagcg tagtaaccgt
    12541 cgcgttcttc cagcatcacc agcatcggca tgtcggtgta gcgacgcaca tagtcggtga
    12601 aatactggct tgggttgtcg aggtggaatt cacgcagcat tacgtggccc atcgccagcg
    12661 ccattgccgc atcggtgccc tgtttcggtg ccagccacag atcgcacagt ttggcgattt
    12721 cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt gtaacgcact tcagtaaaga
    12781 agtgagcatc cggggtacgc gtctgcggca cgtttgaacc ccaggcgatg atgtagctgg
    12841 agttatacca gtcagcagat tccggtacgt cagtttgctc gccccaggtt tgcggagacg
    12901 caggaggcag gtcgcagtac cagtcgtaga agcttaagca agtaccgcca atcagcgaga
    12961 gatagcgtgc acccgatgcg taagaaacca tcgacattgc cggaattggc gagaaaccag
    13021 caacacggtc cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg atcagttcgt
    13081 tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc acgcgcctgc ttaaagcttt
    13141 tcgctttatc ggcgtcttca atgatagaag cccatgcctc aaccggatcg ctatgcagcg
    13201 ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat catcgggtat ttcaggcggt
    13261 tggcactgta aagataccag gagtagctgg caccgcgcgg gcagccgcga ggttcatggt
    13321 ttggcagatc cggacgggta cgcggatagt cagtctgctg ggtttcccag gtgaccagac
    13381 cgtttttgac gtagattttc cagctgcagg agccggtgca gtttaccccg tgggtagagc
    13441 ggacgatttt gtcatgctgc caacgctggc gatatccatc ctcccagtca cggttggtat
    13501 tgagaagctg gccatgccca tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc
    13561 ggtccaggaa tttactcatc ggttttctcc tgtgggagcc tgtcggccct ctgataaatc
    13621 gacattgcta atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa ttgataacga
    13681 tcaagagtga tggggatgaa aaataaagta aattcctaag ataccccttt aggataatta
    13741 acagttacag cataacatgc tgtaaattaa gacgttttat acctgtagtt atttaaacta
    13801 ttaagatttt tatgcaaatg ggtattaagg agtattcccc atgggtaaca tattaatggc
    13861 tcttaatgaa atccggcgaa ataagccata taacactaat atgttgtaac taaatgacgc
    13921 ataaaaaaag cgcggcatca tgccgcgcaa aggataatca aatattaaga tttatttttt
    13981 agaatgccga ccatataccg cccaagtaat cactacgcag gcgatataga aaatcaaaaa
    14041 tactttcatt gcacctactg gcgaacccgt taatgccagc gagctaccaa acgctttcgg
    14101 gataaagaag ccaccaatcg cgccaatcgc agagatgaaa cccagcgccg ctgccgtgcc
    14161 ggttgccgct tcacgcatcg cacgttcgtc agaaccccct tctgctttca cgcgatccat
    14221 tgtcagttta cggaagatca ctgaaatcat ctggaaagtg gaaccactac ccagcccagc
    14281 tgtcaggaac agcgccagga agaccgcgaa gaacgccatg aagcttccgc cctgcccgtc
    14341 agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc atcagaataa agttcaccag
    14401 cgtgacacga gttccgccca gacggtcaga taatgcacca cctgcagaac gcgccagcgc
    14461 accaataaac ggcccgaaga aagcgtattg cagaatctga acatccggga actgcgtttt
    14521 tgacagcata gcaaagcccg cggagaagcc gatgaaggag ccgaaggttg ccagatacag
    14581 caggctcata atccacagat gaccccgttt gagtaccggc aactgctcct tgatggaggc
    14641 tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga tggcaaggaa
    14701 cggcacccat atccaggacg cattcgccag atacagctca gtcccatccg gctgtttgac
    14761 gccctggcta ccaaatactg cgaaaatcga cagtgatacc accagcggag caaccaactg
    14821 catgacgctg acgcccatgt ttcccagacc accattcaga cccagcgcgc caccctgctt
    14881 ctgtttcgga aagaagaagc tgatgtttgc catactggat gcgaagttcg cgccagcaaa
    14941 accgcatagc agagagatga tgatgaagac gctataaggc gtggaggtat cctgcacggc
    15001 aaaacccagc cagacgcaag gaataatcag aataccggtg ctgaacgccg tccagcgacg
    15061 accaccgaag ataggaacca taaaggagta tggaacacgt aataacgcgc cagaaaccga
    15121 aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccga ctttcggtaa
    15181 gttcaccgca acagcgctga acaacatcca tacgcaaaac gccagcagca gacagggaac
    15241 ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt tgttgccaga acgcaggatc
    15301 ttccggtcgc caatctgtta tgacagctcc agtagccctt tcggaggcag atgagtgact
    15361 catagacacc tctgatactc gtttcgatgt ctgccacctt agtgtctgga gctaaaggca
    15421 atttgatgta aatcaaacga taagataact ttatctatca ttgatattta tcattaccca
    15481 tagtgagtac agtgatttca taaaaattat gagatttttc acggtgctgt agaatcccta
    15541 cccttaccga tgtaaagcga ctaaccacac ggcaaataag gagtaactct ttccggctat
    15601 gggtatactt cagccaatag ccgagaatac tgccattcca gaatatatcg tcacattcat
    15661 taaggttatt gctcatttaa agcctgaagg aaggggttta catgcttaaa cgttgtctct
    15721 ctccgctcac cctggttaat caggttgcgc ttattgtgtt gctttctact gctattggac
    15781 tggcagggat ggcggtttct ggctggctgg tgcaaggcgt tcagggcagc gcccatgcga
    15841 tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct gttggcggca gtgccattaa
    15901 gcgagaaaga caagccctta attaaagaga tggaacaaac ggcatttagc gccgagttga
    15961 ctcgagcagc agaacgagac ggacaactgg cgcaattaca gggtttacaa gattactggc
    16021 gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg agaaacggtg tcagcggatg
    16081 tcagccagtt tgttgccggg cttgatcagc tggtatctgg ttttgaccgc accacggaaa
    16141 tgcgtattga gacagtggta ctggtccatc gggtaatggc ggtatttatg gcacttttac
    16201 tggtgttcac tattatctgg ttgcgggcgc gactgctaca accgtggcgg caactgctgg
    16261 caatggcgag tgccgtcagc catcgcgatt ttacccaacg cgcaaacatc agcgggcgca
    16321 acgaaatggc gatgcttgga actgcgttga acaatatgtc tgcagaactg gccgaaagtt
    16381 atgccgtact tgagcagcgg gttcaggaga aaaccgccgg gctggagcat aaaaatcaga
    16441 tcctctcttt tttatggcag gctaaccgcc gtttgcattc ccgcgccccg ctgtgtgaac
    16501 gcctgtcacc ggtactcaac ggcttacaga atttaaccct gctacgtgat atcgaactgc
    16561 gggtgtatga cactgatgat gaagagaatc atcaggagtt tacctgccag ccagatatga
    16621 cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt attacccgtt ggtgatcgcg
    16681 gcacgaccct gaagtggcgg ctggctgact ctcatacgca gtacggtatt ttgctggcga
    16741 ccctgccgca ggggcgtcat cttagccatg atcaacaaca actggtggat accctggttg
    16801 aacaacttac cgccacgctg gcgctggatc gccatcagga acgtcagcaa cagttgatcg
    16861 tgatggaaga gcgtgccacc attgcgcgcg aactgcatga ttctattgcc caatctctct
    16921 cttgcatgaa gatgcaggtg agttgtttac agatgcaggg cgatgcgctg ccagaaagca
    16981 gccgcgaact gttaagtcag atccgtaacg aactgaatgc atcctgggcg cagttgcgtg
    17041 aattgctcac cacattccgc ttgcagctca ccgagcctgg attacgtccg gcgctggaag
    17101 cgagttgcga agagtacagc gccaaatttg gcttcccggt gaagctggat tatcaattgc
    17161 cgcctcgtct ggtgccttca catcaggcaa tccacttgtt gcaaattgcc cgtgaggcat
    17221 taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt ggtgacggtg gcgcaaaacg
    17281 ataatcaggt caaactgacc gtccaggata acggctgcgg cgtgcctgaa aatgccatcc
    17341 gcagcaatca ctacggcatg ataataatgc gcgaccgtgc gcaaagttta cgaggcgatt
    17401 gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt ggtcaccttt attcccgaaa
    17461 aaactttcac agacgtccaa ggagataccc atgagtaatc aggaaccggc tactatcctg
    17521 ctgattgacg atcacccgat gctgcgaact ggcgtaaaac agcttatcag tatggcacca
    17581 gatatcaccg tggttggcga agcgagtaat ggcgaacagg gtattgaact ggcggaatct
    17641 cttgatcccg atctgatcct gttagatctc aatatgcccg gcatgaacgg tctggaaacg
    17701 ctggataaac tgcgcgaaaa gtccctctca gggcgcattg tggtattcag cgtctctaac
    17761 catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg atggctatct gttaaaagat
    17821 atggaaccgg aagatctgct gaaagcattg catcaggcag ctgctggcga aatggtatta
    17881 agcgaagcat taacgcctgt tctggccgcc agcttgcgcg ctaaccgtgc cactactgag
    17941 cgcgatgtta accagttaac cccacgcgag cgcgatattc tcaagctgat tgcccagggt
    18001 ttgccgaaca agatgattgc ccgccgcctg gatatcaccg aaagcacagt aaaagtgcac
    18061 gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg tggaagcagc ggtatgggtg
    18121 catcaggagc gcattttctg attacggttc ccagcgcagt tcgtcgtttg acaatgcatc
    18181 gaacggttcg acaagcgtta gcgtgatctc attggaggag acacgctgcc cctggttatc
    18241 ttccaccacc acagacaatc gccagtgatt gcttgcccct tccccgttct gccagtcagg
    18301 catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg cccggcgtca aacttaatat
    18361 ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa cgactgcgca tttgtaattt
    18421 cagcggcact gtttcgccag gttttagatc ccacggcggt gtcgccagaa acaccgttaa
    18481 cgtttttcgc tgtcggtact caagagtcgg tagattattt cgctgcggat tgtcatagcg
    18541 actaccacgt aacgactgac tttcggcaac ctcgcccgca gaaagttgtt ttttgagcgg
    18601 tacaccaaag cggtaattaa gattcagccc gaggttattt tgattttcgc cactttcccc
    18661 ctgtttatgc tgggccgtca cagtgactaa tggcacaggg gtgtaattta atcccagact
    18721 caacgcgacg ggattgtgat aacccgtacc aaagttaaac aaatcaacac gatcaccaaa
    18781 atactgttct aggctgacac tggtattgag gtgttgatag aacggcatgc gcatccgagc
    18841 tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg gctgtctgtt catgccatgc
    18901 agcaaacggc tgataaaagt ttgccgataa tcgcaaatat tcgccccacg cttcggcacc
    18961 aaagcccgct cgctgaagat tttcgtcctg caagttgtca taaaaagtgt tataacccac
    19021 cagccagttg ccgcgcgccc agcgttgccc aacgcccaca ttgctcacca acccattatc
    19081 ctgctgagta agaccaagct ggctccaggt gagataacga tcattatctt gtaacggcac
    19141 aaaccagctt ccacgactgc cggtgaaatg tccttcgtta tccactttga cgtcaacact
    19201 ggcatttccc cacggtgata gccaggactc tacgtgctga ttaacctgtt gactaagcgc
    19261 gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg ccagtatcca gcccgttatc
    19321 attcatactg gtttcgccga aatctttcac aatttcagca aagtgttttt ccccatcatg
    19381 attttcggga gccatgccta aatccggcag accatcattg ttattatcaa agggatttgc
    19441 cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg cctgctacaa gcaagagtaa
    19501 ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg tcggtaactt ccggtaataa
    19561 tgaaaaacca tagccttaaa gaataacgat tactggcaac ttttgtcgta ttttctcgcg
    19621 ctttcaggaa aatccttaaa aatgtgaccg attggtcaga cgctgcagaa gcagtaagat
    19681 agaagatgag tgtctaattc cctgaataca ggagtaaata atgcaaaaaa tcgtgatcgt
    19741 tgccaatggc gcaccttacg ggagcgaatc cttgtttaac agcttgcggc tggccattgc
    19801 gttacgagag caggagagca atctggatct gcgtctgttc ctgatgtctg atgcggtcac
    19861 agccgggttg cgcgggcaaa aaccagggga aggctacaac attcagcaaa tgctggagat
    19921 ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc tgtaccgacg ggcgcgggat
    19981 tagcacactt cctctgattg atggggtgga aatcggtact cttgtggaac tggcgcaatg
    20041 gacgctgtca gccgataaag tgctcacatt ttaatgttta agggggcgtc tgacgcccca
    20101 ctatgaggga agtttactca ggcgaatccc ggccttaaca caccatccgg ataattctcc
    20161 gccagtagtt tttttaccga taccagcaaa tcattcagcc catcgtcctg cattcccagt
    20221 ttgataagct cctgttccag tgaaaacagg tattgtgcat tggttcccag cggaccgctc
    20281 gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt cagattcata ttctggatgt
    20341 cgcgggtcca taataaacac aatggcgttt actgtgcgtc catcatcaag atcaagctga
    20401 caccaggttg gcagataaca gccggtaatc atctcgcgct tccacaacag ggttagttcc
    20461 tgctccagcg tctcttctgg tagtcgatag gcgacgcctg tggtgcgtcc gccctctttc
    20521 agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag gcgcaggcaa
    20581 aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt cggtaaactc cagtgccgga
    20641 ttccacatca acgatccata accgaagatc cacaccggtc cctcatcagg tcgacaagcc
    20701 agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc ataagagtga ttcttcaatg
    20761 gcaccaaatg ccgttttaca atcggcattc atcaagaaat cacgcgttat caccttacac
    20821 ctccgccact gcatacttcc ctgcgacaac tttttggtgt cttccagaca tttttaccgc
    20881 tcattttgaa accactaacg ggacaatatg caatccacgg accacttgca aggcaacacc
    20941 gcacgaatag ctttaactaa gctaccggtt ttacgatttt ttatgccatt tatcatcatc
    21001 ccctttggca tattcatgct tcacagcagc ccaggccact ttatgcgcgg tttcttcgcg
    21061 actggcgtca tcacgccgat cttctttatc tttatattga tcccatgtgc tgttgaacgc
    21121 ttctttatag atatcctggg catgagacgg tagaacgtgc tttacgcttt ccggcagatc
    21181 gcttttcgtt ttatacggca tcgttgactc cttttacaaa gttgaacatt aagtatggtc
    21241 aacaatatct actcttgcca aaacataaga attttcatgt cagactgttc ttaagaaacg
    21301 ctaaaacaaa cattgtctaa acgtaatact tatttttatg ccccaatgag taaaaaatcg
    21361 acagcggggt aaaaattcgt aaaatgttac cgatatccag acactttctg ttatttttcc
    21421 tacctatttt ttacatttcc gataaattca tctgcaaagg gataaccata atgtcaaatg
    21481 ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct tattttcccg gttctggcgc
    21541 tggtagtgct gttcctgtgg ggaagtagcc agacactacc agtggtcatt gccatcaatc
    21601 ttcttgcgct tattggtttt ttaagtagcg cctttagtgt tgtccgtcat gcggacgtat
    21661 tagcccatcg cctgggagaa ccttacggtt cgcttattct tagcctttca gtggttattc
    21721 ttgaagtcag tttgatttca gctttaatgg caaccggcga cgccgcgcca acgctaatgc
    21781 gtgatacgct ctattcaatc attatgattg ttaccggtgg gctggttggc ttttcattat
    21841 tgttgggcgg tcgtaagttt gccacccaat atatgaatct gtttggtatc aagcagtatt
    21901 taattgcgct gttccccctg gcgataatcg tactggtatt tccaatggct ctgcctgcgg
    21961 cgaatttttc aaccggtcag gcgttactgg tagcattaat ttctgcggca atgtatggcg
    22021 tatttttgct gatccaggcc aaaacgcatc aaagtttgtt tgtctacgag cacgaagatg
    22081 acagtgatga cgacgacccg caccacggta aaccgtctgc ccatagcagc ctgtggcatg
    22141 ctatctggtt gattatccat ctgattgccg ttattgcagt caccaaaatg aacgccagct
    22201 cgctggagac attgctcgac agcatgaatg cccctgttgc ctttactggc ttcctggtgg
    22261 cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa agcagtgttg aacaaccagg
    22321 ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc aacaatttcg cttaccgtac
    22381 ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt gcagtttgca cttggtgcgc
    22441 cagaaatggt ggtgatggtg gcctctttag tgctgtgcca tatctccttc tccaccggac
    22501 gtactaacgt actcaatggc gcagcgcatc tggcactgtt tgccgcctat ttgatgacga
    22561 tatttgcctg acagttatat ctcctccgat gacggaaatc gggagagata agataaattc
    22621 ctgcttattg tatttcttca tactacctgg acagctcgtt aatcagaatt gcgagcctgt
    22681 accacaacct gcacgaaacc actgcaacag ctgttgaaaa gccggaatgt actctttatt
    22741 gcaaacattt cagggatgaa gtgaagatga attgagacgt agtatccaca ccaagccgga
//
LOCUS       DADLXX010000057        22319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLXX010000057 DADLXX010000000
VERSION     DADLXX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.057"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JNX27_002168"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JNX27_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7883374.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JNX27_002169"
     CDS             831..971
                     /locus_tag="JNX27_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883375.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JNX27_002170"
     CDS             985..1857
                     /locus_tag="JNX27_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY7883376.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNX27_002171"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNX27_002171"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7883377.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNX27_002172"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNX27_002172"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7883378.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNX27_002173"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNX27_002173"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY7883379.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNX27_002174"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNX27_002174"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAY7883380.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNX27_002175"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNX27_002175"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7883381.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="JNX27_002176"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="JNX27_002176"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7883382.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVITLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNX27_002177"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNX27_002177"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7883383.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="JNX27_002178"
     CDS             complement(14044..15384)
                     /locus_tag="JNX27_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7883384.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /locus_tag="JNX27_002179"
     CDS             complement(15386..16738)
                     /locus_tag="JNX27_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883385.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="JNX27_002180"
     CDS             complement(17172..17621)
                     /locus_tag="JNX27_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7883386.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNX27_002181"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNX27_002181"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7883387.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="JNX27_002182"
     CDS             complement(18421..18750)
                     /locus_tag="JNX27_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7883388.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNX27_002183"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNX27_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7883389.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="JNX27_002184"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="JNX27_002184"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7883390.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="JNX27_002185"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="JNX27_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7883391.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5511 a         5916 c         5294 g         5598 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccaag
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac ggtttttcca cccggcccca gaaccacggt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtca ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgattacc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gagcagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttag attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DADLXX010000058        22050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLXX010000058 DADLXX010000000
VERSION     DADLXX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.058"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..530
                     /locus_tag="JNX27_002186"
     CDS             <1..530
                     /locus_tag="JNX27_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7883392.1"
                     /translation="RSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFHECSMHGVK
                     IKPWLPVKWTKELISDYLYGCLLSLYSIGARDIYNMNAGNNVKVAADAFLEIIFSLKN
                     KYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYRPQSQDGE
                     DFRSHLQYKLPLSAH"
     gene            741..1064
                     /locus_tag="JNX27_002187"
     CDS             741..1064
                     /locus_tag="JNX27_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7883393.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            1061..1891
                     /locus_tag="JNX27_002188"
     CDS             1061..1891
                     /locus_tag="JNX27_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY7883394.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1888..2901)
                     /locus_tag="JNX27_002189"
     CDS             complement(1888..2901)
                     /locus_tag="JNX27_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7883395.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(3000..4430)
                     /locus_tag="JNX27_002190"
     CDS             complement(3000..4430)
                     /locus_tag="JNX27_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7883396.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4441..5442)
                     /gene="ltaE"
                     /locus_tag="JNX27_002191"
     CDS             complement(4441..5442)
                     /gene="ltaE"
                     /locus_tag="JNX27_002191"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7883397.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5479..7197)
                     /gene="poxB"
                     /locus_tag="JNX27_002192"
     CDS             complement(5479..7197)
                     /gene="poxB"
                     /locus_tag="JNX27_002192"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7883398.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7330..8298)
                     /gene="hcr"
                     /locus_tag="JNX27_002193"
     CDS             complement(7330..8298)
                     /gene="hcr"
                     /locus_tag="JNX27_002193"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7883399.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPNLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8310..9962)
                     /gene="hcp"
                     /locus_tag="JNX27_002194"
                     /gene_synonym="priS"
     CDS             complement(8310..9962)
                     /gene="hcp"
                     /locus_tag="JNX27_002194"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7883400.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10106..11005)
                     /gene="lysO"
                     /locus_tag="JNX27_002195"
     CDS             complement(10106..11005)
                     /gene="lysO"
                     /locus_tag="JNX27_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7883401.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11462..12157)
                     /gene="aqpZ"
                     /locus_tag="JNX27_002196"
     CDS             complement(11462..12157)
                     /gene="aqpZ"
                     /locus_tag="JNX27_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7883402.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYAEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            12582..14240
                     /locus_tag="JNX27_002197"
     CDS             12582..14240
                     /locus_tag="JNX27_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7883403.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(14237..15193)
                     /locus_tag="JNX27_002198"
     CDS             complement(14237..15193)
                     /locus_tag="JNX27_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7883404.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15344..16459
                     /gene="macA"
                     /locus_tag="JNX27_002199"
     CDS             15344..16459
                     /gene="macA"
                     /locus_tag="JNX27_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7883405.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            16456..18402
                     /gene="macB"
                     /locus_tag="JNX27_002200"
     CDS             16456..18402
                     /gene="macB"
                     /locus_tag="JNX27_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY7883406.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18475..18699)
                     /gene="cspD"
                     /locus_tag="JNX27_002201"
     CDS             complement(18475..18699)
                     /gene="cspD"
                     /locus_tag="JNX27_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7883407.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            19022..19342
                     /gene="clpS"
                     /locus_tag="JNX27_002202"
     CDS             19022..19342
                     /gene="clpS"
                     /locus_tag="JNX27_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7883408.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            19373..21649
                     /gene="clpA"
                     /locus_tag="JNX27_002203"
     CDS             19373..21649
                     /gene="clpA"
                     /locus_tag="JNX27_002203"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7883409.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         5445 a         5523 c         5912 g         5170 t
ORIGIN      
        1 ttagatctgt taaaattaat aacgtaaata ctcattcttt aaaatttatt aactgcaatt
       61 ttttcaatgt tgatatgata agagttaatc tcagcaaatg tctttttcat gaatgtagta
      121 tgcatggtgt caagataaaa ccctggttgc ctgtaaaatg gacaaaagaa ttaataagtg
      181 attatttgta tggctgttta ctatctctgt actctatcgg tgcaagggat atatataata
      241 tgaacgccgg gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc
      301 ttaaaaataa atattgtatt aaactattaa gcgctcagga tcgggcgttt atatacgagt
      361 ttgcaagaat gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt
      421 gttttgcggc ggctgaccaa aaagcaatcc agcggtatag acctcagtca caagatgggg
      481 aagactttag gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt
      541 gcaacataat aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac
      601 ggataatcgt aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga
      661 tcaaaacctt cttcccaaca tggcatatca gtccgataat gctttagaga tcgaaggctt
      721 aacaaacaag gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca
      781 tcgttgaata ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg
      841 atttctttgc cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac
      901 tgcgtaaagc acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg
      961 ccgatgccgt agttggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc
     1021 tgatggttag cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct
     1081 ggtcgctgcc gctctgttac tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga
     1141 gggatatcag cttgataccc gacaccaggc gcaggcggcg tatccgcgca ttaaagtgct
     1201 ggtgatccac tacaccgcag atgattttga tagctcgctg gcgacactga ctgataagca
     1261 ggtcagctcg cattatctga tccctgcggt gccaccgcga tacaacggta aaccgcgcat
     1321 ctggcaactg gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg
     1381 ggcaacgcgc cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa
     1441 atcatccgga gtgaaatatt ttgccccgtt tgaacaggca cagattcagg cgcttattcc
     1501 gctggcgaaa gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc
     1561 ggatatcgca ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc
     1621 gcagcagggg attggtgcct ggccggatgc gcagcgggtg aacttttacc ttgccgggcg
     1681 cgcgccgcac acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta
     1741 tgacgtaaaa cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat
     1801 gcatttccgc ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga
     1861 agcattgctg gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca
     1921 ggcggcagtt ttctcgatgc cttcatccag ggtgatgacc ggctgataac ctaactcttc
     1981 ctgcgcacgc gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt
     2041 cagcggcggc tcttttgctg acttgcggcc taaacgctcc atgctgtggg cgatcatatc
     2101 cagcatcggg taagggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt
     2161 ctgcacgatg ctgcgcagtg tgcgatgctc gccgttggtg atgttgtaca cacgcccgga
     2221 aggtagctta tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg cattttcata
     2281 gtaggtcata tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg
     2341 catcatatgc gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg
     2401 gcgcagaata gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc
     2461 gctggctgct ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt
     2521 aatatcgcga tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt
     2581 acgcacaccc caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag
     2641 atcgaaagcc tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc
     2701 aatgcccgcg agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgtcggaac
     2761 aaactctgcg cccatttttt ccagcaattt acccattgct tcgttgcgac cgctcgcacg
     2821 cacgctgatg cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc
     2881 gccggtaacc agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct
     2941 tccgtgattt cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat
     3001 taatctgtgc tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac
     3061 agatgcgcgg ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag
     3121 aaagcgcgga catcgatagt acgatagtca cctttatctt ccagggtaaa acacagtcgt
     3181 cccagccccg gcgctttcat gccaaataac aacgtaagtt gctttttcgg ttcaacgaca
     3241 atcactttcc agctatccac cgcatcgccg gtctgtaaat attcgcgctc cgggcggcct
     3301 ttcgccagat tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg
     3361 ccaaagaaat aacgctcttt accgccgatt tggttcacca ccagccataa agccgcaagg
     3421 ctggcggacg ttttaacggt aaaccccgcc tgtttggcga aataaccgta ctccggtcgc
     3481 cagcgggcaa aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcttcc
     3541 tctttcaacg tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca
     3601 cgtagcgcgg tatcatctgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg
     3661 gtggtgggcg gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg
     3721 agggggatgg ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt
     3781 tgctgataac tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gttggctgga
     3841 tggtctaaca gcgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt
     3901 gaacgtaccc agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact
     3961 tcgaacgccg ctgaacctgc gccaacgata attccggccc gaagttcggt cactggtaca
     4021 ttcgcttcac gaagaatgtc cgccgtagcc tgacgagctc gcagatgatc cgactgctca
     4081 tgtggcgggg cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc
     4141 gcatcgcgga cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc
     4201 atgctgtgca ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc
     4261 ggccagctga gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg
     4321 tcgacatgac gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga
     4381 tgctgaccaa tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc
     4441 ttaacgcgcc aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac
     4501 gtcaagatgc gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt
     4561 catgtattcg cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt
     4621 gtcctgacgc atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt
     4681 gtcgtgatct tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc
     4741 cagaatcccg gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt
     4801 aatgtaatca cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag
     4861 gcaaatggtg aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac
     4921 cacggcatta aagatgtgcg caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa
     4981 ttcccatgct tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttccag
     5041 actgagtaat ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt
     5101 atccagcggt agcgtgccgt cggcagccgc gtcgatgggt tgcggctgaa tactgcccag
     5161 caccgctgcg ccaccggctt caaacagata gttatgcgcg gcctgaccga caatatactc
     5221 ttcgccacgt tcgcagtgac tgagcagagc gaccaggttg gcctgagtgc cggtaggcag
     5281 aaaaatggcg gcttctttac cggaaagctc tgctgcatag tcctgcagag cattaacggt
     5341 agggtcgtct ccgtaaacgt cgtccccaac cggggcggcc atcatcgctt cgagcatggc
     5401 gcggctcggt cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga
     5461 cgggaaatgc cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc
     5521 gccgcgtccg ctgataattg cgcgcaacat atacaggcta aaacctttgg cctgttcgag
     5581 tttgatctgc ggtggaatgg ctaattcttc tttggcgacc accacatcca ccaacaccgg
     5641 accgtcgata gagaaggcgc gttgcagggc ttcatcaact tcagaggctt tttctacacg
     5701 gatacccgta atgccgcacg cttcggcaat gcgggcaaag ttggtgtcgt gcagctcagt
     5761 accgtctgtc aggtaacctc cggccttcat ctccatcgcc acaaagccca gcacgctgtt
     5821 attaaagacg ataattttca ccggcagttt catctgcact actgagagga aatcgcccat
     5881 caacatgcta aaaccgccat cgccgcacat ggcgaccacc tgacgttccg gctcggttgc
     5941 ctgcgcaccc aacgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa
     6001 caggcgacgc ttgccgttca ttttcagata acgtgccgcc cataccgttg gcgtgccgac
     6061 gtcacaggta aagatggcgt catcggcggc aaaatgacta atttgctgcg ccagatattg
     6121 cgggtgaatg gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcatcgcg
     6181 gtaatcttcc agcgctttat ccagaaactt gcgatcggtt ttttcttcca ccagtggcag
     6241 caatgcgcgc agggttgatt tgatatcgcc gaccagcgcc atatctacct tgctatgcgc
     6301 gccgatgctg gctggattaa tatcaatctg aataattttg gcatcggtcg ggtagaacgc
     6361 acggtagggg aactgagtac cgagtagcac taacgtgtcg gcgttcatca tggtatggaa
     6421 acctgacgag aatccgatta acccggtcat tccaacatca tacggattat cgtattcgac
     6481 atgctcttta ccgcgtaggg catgaacaat aggtgcttta attttcccgg caaactcaac
     6541 taactcttta tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata
     6601 acgcagcagt tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggctgtgg
     6661 cgcatgatac cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcccgg
     6721 taacacaaca acggaaacgc cacggttaag caccgctttg cgcatagcaa ttgccagcac
     6781 ttgtgggatc tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag
     6841 ctcttgtggg tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc
     6901 ggcaatcgcc agtaccggaa cgttattgcg gtggcaatcg aataggccgt tgatcaagtg
     6961 caggttgcct gggccgcacg atccggcaca gaccgccagt tctcctgaaa gttgtgcttc
     7021 agcgccagcg gcaaacgccg ccacttcttc atggcgggtg gacatccact cgatggtgcc
     7081 catgcgatta agactgtcgc taagaccgtt cagagagtcg cccgtgactc cccagatgcg
     7141 tttcacgcct gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt
     7201 tctccatctc ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg
     7261 gatgggagag aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc
     7321 ataaggcgat tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta
     7381 accttcagcg atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga
     7441 aaccactttt gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac
     7501 gttattgctt tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc
     7561 cggttgcagt ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gggtgaagaa
     7621 tttctcttta aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat
     7681 atacggagcc gggccgcagg tcatcacggt acgtgaagct aagttaggta catctgccag
     7741 cagttcgcga gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag
     7801 cgttaccgga tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt
     7861 gtagatcacc cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat
     7921 aatcggcgtg acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc
     7981 gcaggtaaat tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg
     8041 cgtcagccac tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc
     8101 actcacgcct ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac
     8161 gctgaccagt gcatattgcc cggcgcgata tgggtagtga tcgtggcaaa tcagggaaat
     8221 cgtccacaca tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg
     8281 attcgttggc atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct
     8341 tcaacagtgg tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt
     8401 gtcaggaaac taggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc
     8461 gtcagcagaa tgacaatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga
     8521 tcgttcacac cacagcccag tttctctgcc agagtgacag ccagaataat tgctgagtaa
     8581 gcatcgttac attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca
     8641 agtttgttaa agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg
     8701 ctggtggcga aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc
     8761 aggaagatat gacgcagttt ttcacggcta accagatcaa tcagcgtatc ggctgcacca
     8821 agcagcgtct ggcgaccaaa gcccacggtg atcaggtgag gaatttcgct gtacgggaag
     8881 cccgccattt gttgcgcctg ggcgatgacc gcagagaaat cttcgccatc cagatgacgc
     8941 acgccaggcc agccaacgat gctgcgggtc cagatacgat cgtcataagc gcctacggtt
     9001 gggtcgatga tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact
     9061 tgctgattct gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc
     9121 gggtagccat gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg
     9181 gtctgttcca gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta
     9241 cccgccgtcg ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg
     9301 cctgcatcca gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca
     9361 agaagcgcgt tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg
     9421 gcataaatat cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca
     9481 cctttcaggc catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct
     9541 ttatctttgt taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc
     9601 accagttgca ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat
     9661 tgtgctttca gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga
     9721 gaatcgaagt taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca
     9781 tcgtggttga tgatgccgta ttcacgcgct tttaccgccc aggcagaaag cccttgcagc
     9841 gccgcgatga gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc
     9901 gcgtatgagc agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac
     9961 atgatcacac cttttaaagt tatatttaat atacatgttt aaggttaaga tgcttaacgc
    10021 ggggataaaa gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg
    10081 gcagggatct accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta
    10141 acaggctaag aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac
    10201 gttgaagaac gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag
    10261 agcggctgcg gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat
    10321 cattaaaaaa cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg
    10381 aaagagaata ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa
    10441 gaataaaggc gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa
    10501 tcattccccg gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa
    10561 taccaacgag gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta
    10621 agaaagccag tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta
    10681 gcgactccag cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc
    10741 acggcaggcc tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa
    10801 tactgacggc agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg
    10861 ccagactgat acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa
    10921 taacttttaa cgcagcttgt tggcgaagcg gaatgaggta acccacaatc aggggaacca
    10981 gaatgattaa cagcccagaa aacatgaaaa tccagtcctt gcaaagaaga agtcgaaatg
    11041 cgcgatgaca cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt
    11101 atgcatcaga gggggatttt taatcctatc tgagtgttta gcattgttgc gctcctttca
    11161 tgtgcaatgc catataaagc cactggcgga gggagcgcgc agggggcagc cagtcgccgc
    11221 cgccccctgc acccccgggc tctggcgaac aaaatcgccg ctgcgcggta ccttcagctt
    11281 atccccttcg gcaaccaggt cgggcgcgag gtaacgtcct gtaagacgcg ccctcagccc
    11341 acatccatgt gggctgcccc ggccttcggg gaacgcttca gcgattttga cgccaccaat
    11401 acccgtgcgg tttgtaatgt cgaagataaa acaaccaggg cagataaaat gccaatgcta
    11461 attaatcacg cttttccagc agagtccggt aaatcagacc accgataatg ccgccgacaa
    11521 ttggcaccac ccagaagaac cacagttgtt ctaatgccca gccgccctgg aagatagcaa
    11581 ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac cggaatactg attaagtgaa
    11641 tcagggttaa ggccagacca atagcgatcg gcgcaaaacc tgccggagcg aatttgtcgg
    11701 ttgcaccgtg gatcactaac aggaagcccg cactcaatac cagttcaact accagcgcgg
    11761 aaagcatgga ataaccgcct ggtgaatgct cggcataacc gttagaagca aaaccgctgg
    11821 ctgccgcgtc aaaacccgtt ttaccactgg caattaaata cagcagcgcc gctgcaacaa
    11881 taccgccgac aacctgggca atcacgtagc caacgacttc ttttgccgga aaacgtccgc
    11941 cagcccataa accaatagtg accgccgggt taaaatgacc accagaaata tgaccaacag
    12001 cgaaggccat cgtcagaacg gtcaaaccaa atgccagcgc cacgccggca aaaccaatgc
    12061 ctaattccgg gaagcctgcg gccagtacag cactaccaca gccaccaaaa acaagccaga
    12121 aagtaccaaa acattcagct gctaattttc tgaacatatc cacctcaatt aaaaattgac
    12181 cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa aatgacgacg gaaataatgc
    12241 gcggctattt taaaaacgaa ggcgagtcat tcaccagata aataaatcca gtaaatttga
    12301 tttagggcaa cagcgggttg ccccatatag tcatttgtct gattgacagt gtagtgcaag
    12361 caaaagattt aatcctttag gcgtaataaa aaataattta tcatgctaat tatttgattt
    12421 tgttgttttt gcgggcttat cagcaagagg gagtataacg cgattattcg ctcatttttc
    12481 agacaattgc catgcttaaa tgtgatgtca tcacgtatta gcaaggcctt tcccgttata
    12541 ctgccagcgt aaaggataag tcacatattt ctggagggga tatgattctt gagcgcgttg
    12601 aaattgtggg ttttcgcggt atcaaccgtt tgtcgttgat gctcgaacaa aacaacgtcc
    12661 tgattgggga gaacgcgtgg ggtaaatcca gcttgctgga cgccttaacc ctgctgctat
    12721 cgccagaatc agatctctac cattttgagc gcgacgattt ctggttcccg ccgggagata
    12781 tcaacgggcg agaacatcat ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc
    12841 gacatcgggt tcgccgttat cggccgctgg aagcgtgctg gacgccatgc accgatggct
    12901 atcaccgtat tttttatcgt ctggaagggg agagtgcgga agacggcagc gtgatgacac
    12961 tgcgcagttt tctcgataaa gacggacatc cgattgatgt tgaggatatt aacgatcagg
    13021 cacgccatct ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc
    13081 gtattcgtaa cggcacggtg ccaaatgtcc ccaatgtgga agtcaccgcg cgccagctcg
    13141 atttcctcgc ccgtgagttg tcctcacatc cgcaaaatct ctctgatgga cagattcgtc
    13201 agggactttc cgcaatggtg cagttgcttg agcattattt ctctgagcag ggggctggac
    13261 aggcgcgata tcgtttaatg cggcggcggg ccagcaatga gcaacgaagc tggcgctatc
    13321 tggacatcat caaccggatg attgaccgac ctggagggcg ctcgtatcgg gttattttgc
    13381 tcggcctgtt tgctacttta ttgcaggcaa aaggcacatt gcgactggat aaagacgcgc
    13441 gtccgttatt gctaattgaa gatccagaaa cccgcctgca tccgattatg ctttccgttg
    13501 cctggcatct gttgaatctt ttgcctttac agcgtattgc taccaccaac tctggcgagc
    13561 tgctttcgtt aactccagtg gaacatgttt gccgcctggt gcgtgaatct tctcgtgttg
    13621 ccgcctggcg tctggggcca agtggcttga gcaccgaaga cagccgccgc atctcttttc
    13681 acattcgttt taatcgtccg tcatcgctgt ttgctcgctg ctggttgctg gtggaagggg
    13741 aaacggaaac ctgggttatc aatgaactgg cgcgtcagtg cggacatcat tttgatgccg
    13801 aagggatcaa ggtcattgag tttgcccagt ccgggctaaa gccactggtt aaatttgccc
    13861 gccgaatggg gattgaatgg catgtactgg tcgatggcga tgaagcaggg aagaaatatg
    13921 ccgctacggt acgcagcctg ttgaataacg atcgggaagc cgaacgagaa catttaacgg
    13981 cgttaccggc gctggatatg gaacatttta tgtatcgcca gggattttcc gatgtgttcc
    14041 accgcgtggc gcaaatcccg gaaaatgtac cgatgaatct acgcaaaatt atctcgaaag
    14101 cgatccatcg ctcttccaaa cccgatcttg ccattgaagt ggcaatggag gcaggacgtc
    14161 gtggtgtgga ctccgtacca acgctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg
    14221 cgcgcggtcg cgcggattaa ccgcgaaaca tcgtggccat ttgtggctga atagcgtcga
    14281 gcatctcata gcgccgacgg tattcagccc gttttttact ggcgatttcg gcaatctctt
    14341 ttcgtgctat ctgtgctgga aggcggtaat ggcgttcagc atcacatacg ccgccaaccg
    14401 actcccagaa cgcgttgtaa tcagcatgga tcttgccttc tttatcgcga taacgcaggc
    14461 tgcggtaaat atgcgtttca ttgctgacgg caataatctg ctctacctgc aaacgttggg
    14521 caaacagaca ggccgcttcc atcacgaggc gtttgggaaa tagcccgtgg caggctttcg
    14581 tcgcattctg gatttcctga tgtggaattt cccattttgc gccttgcagt ccgccaataa
    14641 acatcgttct tttcccctga tattcacaca gggtaaacgt gatctctgcc agaggaatac
    14701 cttcgctgtt gcggaacagg attgtgctgt caccttcttt atccattgag atcatcatgg
    14761 tcagctcaag cgtgaactgc tcaccgtttt tgccttccag tttagccagt tgcagcccgg
    14821 gggtattcaa atataagctg aattcttccg ccgacataca cccgcggagt aacgcataat
    14881 ggtaacgtaa cgcctccagc aattgcttac ggctaagatt cgccgcaagg taagggcgat
    14941 gcagacgcac aggcagtcgc ggctggcgcg ttaacaacac attgagatta ggccagtggg
    15001 aaagttcgtt catccactca acgcttaaac gcggcataat caacgagcgc agcaaaaatt
    15061 tctggcgaaa actacagcga tgccagaatt tacccggccg acactgtcca cgtgccagac
    15121 taagaaaaag tgacaggctg ctgagagatt cagatggcgt aaaggtccgt tcagttagct
    15181 gcgacatatt catgaaatca atggttatac atgacgtcga tttcaccatt gcgtatctta
    15241 accaaacatc aatagtgtga ttactaacgt aaattttagg attttgttga tatttcgttg
    15301 aagttaatga cccggattgg catgtggagt attcagaata tttatgaaaa agcggaaaac
    15361 cgtgaagaag cgttacgtta ttgcgctggt gatagtcatc gccggactga ttacgttatg
    15421 gagaattctt aacgcacccg tgccgactta tcagacactg attgtgcgcc ccggtgattt
    15481 acagcaaagc gtgctggcga ccggaaagct ggacgcgctg cgtaaggttg acgtaggcgc
    15541 gcaggtcagc ggtcagttga aaacgctgtc ggtggcgatt ggcgataaag taaaaaaaga
    15601 ccagctttta ggggtcattg atcctgaaca ggctgaaaac cagatcaagg aggtcgaagc
    15661 aacgctgatg gagctacgcg cgcagcggca gcaggcggaa gcggagctga aactggcgcg
    15721 ggtgacgtat tcccgtcagc aacgtctggc acaaacgcaa gcggtttcac tgcaggatct
    15781 cgataccgcc gcgacggaga tggctgtgaa acaggcgcaa attggcacca ttgacgcgca
    15841 aatcaagcgc aatcaggcct ctctcgatac ggctaaaacc aatctcgatt acacccgtat
    15901 cgttgccccg atgggcgggg aagtgacgca aatcaccact ctgcaaggcc agacggtgat
    15961 tgccgcacag caagcaccga acattctgac gctggcagat atgagcacta tgctggtaaa
    16021 agcgcaggtt tctgaagcgg atgtaatcca cctgaagccg gggcaaaaag cctggtttac
    16081 ggtgcttggc gatccactga cgcgctacga ggggcaaatc aaggatgtgc taccgacgcc
    16141 ggaaaaggta aacgacgcta ttttctatta cgcccgtttt gaagtaccca accccaatgg
    16201 tttgctgcgg ctggatatga ccgcgcaagt gcatattcag ctcaccgatg tgaaaaatgt
    16261 gttgacgatc cctctgtcgg cgttaggcga tccggttggc gataatcgtt ataaagtcaa
    16321 attgttgcgt aatggtgaaa cacgcgagcg tgaagtgacg attggcgcac gtaacgatac
    16381 cgatgttgag attgtcaaag ggctggaagc gggcgatgaa gtggtgattg gtgaggccaa
    16441 accaggagct gcacaatgac gcctttgctc gaattaaagg atattcgtcg cagctatcct
    16501 gccggtgatg agcaggttga ggtgctgaag ggcatcagcc tcaatattta tgcgggtgag
    16561 atggtcgcga ttgttggcgc ttcgggttcc ggtaaatcga ccctgatgaa tattctcggc
    16621 tgcctggata aggccaccag cggcacctat cgcgtagccg gtcaggatat tgccacgctg
    16681 gacgccgatg cgctggcgca actgcgccgc gagcatttcg gctttatttt ccagcgttac
    16741 catttgcttt cgcatttaac cgccgagcag aacgttgaag tacccgccgt ctatgccggt
    16801 cttgagcgga aacagcgact gcttcgtgcc caggagttgc tgcaacggct ggggctggaa
    16861 gaccgcacag agtattatcc ggcacagctt tcgggtggtc agcaacagcg cgtcagcatc
    16921 gcgcgggcat tgatgaacgg cggtcaggta attcttgccg atgaaccgac cggcgcactg
    16981 gacagccatt ctggcgaaga ggtgatggcg atcctgcatc agctgcgcga tcgcgggcat
    17041 acggtgatta tcgtcaccca cgatccgcag gtcgctgctc aggccgagcg ggtgatcgaa
    17101 attcgcgacg gcgaaattgt gcgcaatcct cccgccattg aaaaagtgaa tgttgctggc
    17161 gggacggagc ctgttgtcaa tacagtgtct ggctggcggc agtttgtcag cggttttaac
    17221 gaggcgctga cgatggcatg gcgggcgctg gcagcgaata aaatgcgtac tttactgacc
    17281 atgctgggga ttattatcgg tattgcgtcg gtggtttcca ttgtcgtggt gggtgacgcc
    17341 gccaaacaaa tggtgctggc ggatattcgt tctattggta cgaatactat tgatgtctat
    17401 cccgggaaag attttggcga tgacgatccg caatatcagc aggcgctgaa gtacgacgac
    17461 ttaatcgcca tccaaaaaca accgtgggtc gcctcagcca cacctgccgt ctcgcaaaac
    17521 ctgcgcctgc gttataacaa tgttgatgtt gctgccagtg ccaatggcgt gagcggcgat
    17581 tattttaatg tctatggcat gaccttcagt gaaggaaaca cctttaatca ggagcagctg
    17641 aacggtcgtg cgcaggtcgt ggttctcgac agtaatactc gccgccagct tttcccccat
    17701 aaagcagatg tggttggcga ggtgattctg gtcggcaata tgcccgccag agtcattggt
    17761 gtggcggaag aaaaacagtc gatgtttggt agcagtaaag tgctgcgtgt ctggctacct
    17821 tacagcacga tgtccgggcg agttatgggc cagtcgtggc ttaactccat tactgtcagg
    17881 gtgaaagaag gatttgacag cgccgaggcg gaacagcaac tcacgcgttt actttcactg
    17941 cgccacggta agaaggattt ctttacctgg aacatggacg gcgtcttgaa aactgttgaa
    18001 aagaccacac gtactttaca actgtttctg acgctggtgg cggtgatttc gctggtggtg
    18061 ggcggtattg gtgtaatgaa tattatgctg gtgtcagtga ccgagcggac gcgggaaatt
    18121 ggcattcgca tggctgtagg tgcgcgagca agcgatgttt tgcaacagtt cctgatcgaa
    18181 gccgtactgg tttgcctggt cggtggcgcg ttgggaataa cactgtcact gttaattgct
    18241 ttcaccttgc agcttttctt acccggctgg gagattggtt tttcaccgtt ggcgctgctg
    18301 ctggcgtttc tctgctcgac ggccaccggg attttatttg gctggttacc cgcacgaaat
    18361 gcggcacgac tggatccagt agatgctctg gcacgggagt aatttttgag ataaaaatgc
    18421 cagccgatcg ggctggcatt ttgcctttag gatgtacaca atgagacaga agagctatgc
    18481 gactgccgct tctacttcga cgggcacaat aacactggcg tgattgcctt ttggcccctg
    18541 gtggacatca aactgaacgg attgtccagc ttttagcgtt ctgtaaccat ccatctgaat
    18601 ggtggaataa tgagcgaaaa tatcttcgcc gccgccttca gggcagatga aaccaaaacc
    18661 tttggcattg ttgaaccact taacagtacc cttttccatg cttcgacatc cttcgcaaat
    18721 cttatacaag taagatggaa taaaccgggg tcagagaggg ggctgttcaa aacctcgcca
    18781 actctagaaa tacaatttag agaattaggg cgagccgtca agcatttgac aggggccagg
    18841 gggcaggtat gaatcaaaaa tttgaagcag ttaacgctat tgacaggaat gtgacagatg
    18901 tcgctgatgc caacgataga tgatagttat ctatcatgtg gagtagattg gtcaggcaaa
    18961 taagctcttg tcagcggcag ggcgttctgc cgataaccgt aaccgaagat gataactgac
    19021 aatgggtaaa acgaacgact ggctggactt tgatcaactg gcggaagaaa aagttcgcga
    19081 cgcgctaaaa ccgccatcta tgtataaagt gatattagtc aatgatgatt acactccgat
    19141 ggagtttgtt attgacgtgt tacaaaaatt cttttcttat gatgtagaac gtgcaacgca
    19201 attgatgctc gctgttcact accaggggaa ggccatttgc ggagtcttta ccgccgaggt
    19261 tgcagaaacc aaagtggcga tggtgaacaa gtacgcgagg gagaatgagc atccattgct
    19321 gtgtacgcta gaaaaagcct gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa
    19381 tcaagaactg gaactcagtt taaatatggc tttcgccaga gcgcgcgagc accgtcatga
    19441 gtttatgacc gtcgagcact tgttactggc gctgctcagt aacccatctg cccgggaggc
    19501 gctggaagcg tgttctgtgg atttggttgc gctccgtcag gaactggaag cctttattga
    19561 acaaaccaca cccgttctgc ctgccagtga agaggagcgc gacacacagc cgacgctgag
    19621 ttttcagcgt gtactgcaac gtgcggtctt ccatgtccag tcctccggtc gcaatgaggt
    19681 taccggtgca aacgttctgg tcgctatctt cagcgaacag gagtcgcagg cggcatatct
    19741 gttgcgtaaa catgaagtca gccgtctcga tgtggtgaac tttatctctc atggcacgcg
    19801 taaagacgag ccgacacagt cttctgatcc tggcagccag ccaaacagcg aagaacaagc
    19861 tggtggggag gaacgtatgg agaatttcac gacgaacctg aaccagcttg cgcgtgtggg
    19921 cggaatcgac ccactaattg gtcgtgagaa agagctggag cgtgctattc aggttctctg
    19981 ccgtcgccgt aaaaacaacc cgctgctggt gggggaatct ggtgtcggta aaaccgcgat
    20041 tgcagaaggt cttgcctggc gaattgttca gggtgatgtg ccggaagtga tggctgactg
    20101 tacgatttac tctctcgata tcggttctct gttagcgggc acaaaatatc gcggcgactt
    20161 tgaaaaacgt tttaaagcgt tgctcaagca gctggagcag gacactaaca gcatcctgtt
    20221 tattgatgag atccacacca ttatcggtgc gggtgcagcg tctggtggtc aggtcgatgc
    20281 ggctaaccta atcaaaccgt tgctctccag cggtaaaatt cgcgtaattg gttcgacaac
    20341 ctatcaggag ttcagcaaca ttttcgagaa agaccgtgct ctggcgcgtc gcttccagaa
    20401 aattgatatt actgaaccgt cgatcgaaga aactgttcaa atcatcaatg gcctgaaacc
    20461 gaagtatgaa gcgcaccacg acgtgcgtta taccgcaaaa gcggtgcgtg cggcggtaga
    20521 gctggcggtg aaatacatta acgatcgtca tctgccggat aaagccattg atgttatcga
    20581 cgaagcgggc gctcgcgcac gtctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt
    20641 ggcggatatt gagtccgtgg tggcccgtat tgcacgcatt ccagagaaga gtgtttctca
    20701 gagtgaccgc gataccctga aaaacctcgg cgatcgcctg aaaatgctgg tcttcggcca
    20761 ggataaagcc attgaggcgc tgactgaagc cattaagatg gcgcgtgcag gtttaggtca
    20821 cgaacataaa ccggtcggtt cgttcctgtt tgccggtcct accggggtcg ggaaaacaga
    20881 ggtgacggta cagctttcga aagcgttggg cattgagctt ctgcgctttg atatgtccga
    20941 gtatatggaa cgccataccg tcagccgtct gattggtgcg cctccgggat acgttggttt
    21001 tgatcagggc ggtttgctga ccgatgcggt aatcaagcat ccacatgcgg tgctgctgct
    21061 ggacgaaatc gagaaagcgc atccggacgt gttcaatatt ctgttgcagg tgatggacaa
    21121 cggtacgctg accgataaca acggacgcaa agcggacttc cgtaacgtgg tgctggtgat
    21181 gaccactaac gccggggtac gggaaactga gcgcaaatcc attggtctta tccaccagga
    21241 taacagcacc gatgcgatgg aagagatcaa gaagatcttt acaccggaat tccgtaaccg
    21301 tctcgacaac attatctggt tcgatcatct gtcaactgac gtgatccatc aggtggtgga
    21361 taaattcatc gtcgagttgc aggttcagct ggatcagaaa ggtgtttctc tggaagtgag
    21421 ccaggaagcg cgtaactggc tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc
    21481 gatggcgcgt gtcatccagg acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg
    21541 ttcgctggtg gacggcggtc aggtgacggt tgcgctggat aaagagaaaa atgagctgac
    21601 ttacggattc cagagtgcac aaaagcacaa ggcggaagca gcgcattaat ctgattgtca
    21661 ggtaggttgg tgaagtccgt aatctcgtca ggggttacgg actttttatt tatggggtgg
    21721 aacggtgaaa accctatttt tggaggtgaa ggtaagttgt tgataattag tgctgctgga
    21781 aggtaaggat aaaaaagggt gctgcaggag aatgggatgg ttttgcttta ttaacaacgg
    21841 gctaaacgtg tagtatttga gttcactgcc gtacaggcag cttagaaatc gacggggtgc
    21901 ggtaaaacct ttgcgaacgc gttcactgcc gtacaggcag cttagaaatt cacaggtaac
    21961 atactccacc cgcccaccat gttcactgcc gtacagacag ataaaatgcg aaaaaaaagc
    22021 tcgcactttc gtacgagctc ttctttaaat
//
LOCUS       DADLXX010000059        21667 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLXX010000059 DADLXX010000000
VERSION     DADLXX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.059"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(136..1026)
                     /locus_tag="JNX27_002204"
     CDS             complement(136..1026)
                     /locus_tag="JNX27_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY7883410.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1056..2312)
                     /gene="wzx"
                     /locus_tag="JNX27_002205"
     CDS             complement(1056..2312)
                     /gene="wzx"
                     /locus_tag="JNX27_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY7883411.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(2328..2873)
                     /gene="rfbC"
                     /locus_tag="JNX27_002206"
     CDS             complement(2328..2873)
                     /gene="rfbC"
                     /locus_tag="JNX27_002206"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7883412.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(2878..3756)
                     /gene="rfbA"
                     /locus_tag="JNX27_002207"
     CDS             complement(2878..3756)
                     /gene="rfbA"
                     /locus_tag="JNX27_002207"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7883413.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(3815..4714)
                     /gene="rfbD"
                     /locus_tag="JNX27_002208"
     CDS             complement(3815..4714)
                     /gene="rfbD"
                     /locus_tag="JNX27_002208"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7883414.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(4714..5799)
                     /gene="rfbB"
                     /locus_tag="JNX27_002209"
     CDS             complement(4714..5799)
                     /gene="rfbB"
                     /locus_tag="JNX27_002209"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7883415.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(6172..7065)
                     /gene="galF"
                     /locus_tag="JNX27_002210"
     CDS             complement(6172..7065)
                     /gene="galF"
                     /locus_tag="JNX27_002210"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7883416.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7240..8634)
                     /gene="wcaM"
                     /locus_tag="JNX27_002211"
     CDS             complement(7240..8634)
                     /gene="wcaM"
                     /locus_tag="JNX27_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7883417.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(8645..9865)
                     /gene="wcaL"
                     /locus_tag="JNX27_002212"
     CDS             complement(8645..9865)
                     /gene="wcaL"
                     /locus_tag="JNX27_002212"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7883418.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(9862..11142)
                     /gene="wcaK"
                     /locus_tag="JNX27_002213"
     CDS             complement(9862..11142)
                     /gene="wcaK"
                     /locus_tag="JNX27_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7883419.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(11322..12800)
                     /gene="wzxC"
                     /locus_tag="JNX27_002214"
     CDS             complement(11322..12800)
                     /gene="wzxC"
                     /locus_tag="JNX27_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7883420.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(12802..14196)
                     /gene="wcaJ"
                     /locus_tag="JNX27_002215"
     CDS             complement(12802..14196)
                     /gene="wcaJ"
                     /locus_tag="JNX27_002215"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7883421.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14251..15621)
                     /gene="cpsG"
                     /locus_tag="JNX27_002216"
     CDS             complement(14251..15621)
                     /gene="cpsG"
                     /locus_tag="JNX27_002216"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY7883422.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(15726..17162)
                     /gene="cpsB"
                     /locus_tag="JNX27_002217"
     CDS             complement(15726..17162)
                     /gene="cpsB"
                     /locus_tag="JNX27_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY7883423.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17165..18388)
                     /gene="wcaI"
                     /locus_tag="JNX27_002218"
                     /pseudo
     CDS             complement(17165..18388)
                     /gene="wcaI"
                     /locus_tag="JNX27_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(18385..18864)
                     /locus_tag="JNX27_002219"
     CDS             complement(18385..18864)
                     /locus_tag="JNX27_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7883424.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18864..19832)
                     /gene="fcl"
                     /locus_tag="JNX27_002220"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(18864..19832)
                     /gene="fcl"
                     /locus_tag="JNX27_002220"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7883425.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(19835..20956)
                     /gene="gmd"
                     /locus_tag="JNX27_002221"
     CDS             complement(19835..20956)
                     /gene="gmd"
                     /locus_tag="JNX27_002221"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7883426.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(20982..21530)
                     /gene="wcaF"
                     /locus_tag="JNX27_002222"
     CDS             complement(20982..21530)
                     /gene="wcaF"
                     /locus_tag="JNX27_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7883427.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21546..>21667)
                     /locus_tag="JNX27_002223"
     CDS             complement(21546..>21667)
                     /locus_tag="JNX27_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY7883428.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         5396 a         5712 c         5002 g         5557 t
ORIGIN      
        1 tcagttgata aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata
       61 ctttttaatt gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact
      121 cttttttgat cagaactatg cattataacg accgccccca gtaattcctc ttattccatc
      181 cagcgtagcc aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat
      241 taaagtcgta tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg
      301 attttttcta agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg
      361 aattggtatg ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc
      421 tatatcagga atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt
      481 atcgacataa tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt
      541 tgaaaccatg gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt
      601 gacatgaatt ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga
      661 cttatctcta tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca
      721 ggaaagtttt ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt
      781 ctctgcccct ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc
      841 attcctgcca agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt
      901 gaaattagtc ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat
      961 cagtgcattc agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact
     1021 attcattatc caagtgactc agtaattggt taattttagt tttcatatac agaacacatt
     1081 ctagtataaa gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt
     1141 gcggcaccca gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc
     1201 aattcagcac aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag
     1261 aatggtatca ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta
     1321 aaggaattaa tttggtaatt ctcaccaaac catattttca atacaagccc tgaatataac
     1381 aagcctatga ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca
     1441 ttacgtttaa attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg
     1501 attgctgcag gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca
     1561 actttatctg cccccataat atttgacact ataaatcgat caaaatacac catgaccgga
     1621 cttatgatat tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta
     1681 taacaaaaat gaacccccga aattttaata tcattcctga cataataggc agagactaaa
     1741 atcgaaataa ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa
     1801 aaaacaaata tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt
     1861 acaatgccaa atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat
     1921 aatggaatgc aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca
     1981 gaaattttta gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag
     2041 catgaaaata gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga
     2101 ttatcacgat gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt
     2161 ccagcatacc caacaagtgc aattgcaatt gtataaacac cgaaaccttc aggccccaaa
     2221 cctcgagcga gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa
     2281 aggttccaga cactattttt tattatgctc attattacat ttctttatca tataaaataa
     2341 tcagcatcta caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc
     2401 ggccattcta tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa
     2461 tagaaattat ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga
     2521 gcgaatcctt ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc
     2581 cacttgccaa atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg
     2641 acaacacagc gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt
     2701 aacacaccct tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca
     2761 gcctcttcga aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat
     2821 acttttggtt caaaaattag cacatcagga atttcagttt taattacgtt cattttatta
     2881 ataaccttta atcattttca gcagatactg accataagca tttttcttca gcggctcggc
     2941 taataccttc acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca
     3001 ggaaactttc agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag
     3061 actttgatgt gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa
     3121 acgcccttgg tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga
     3181 tggtttaagg tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac
     3241 cgcatagtta ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc
     3301 atcaaattcc accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc
     3361 gccactctct ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat
     3421 attatcacca agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa
     3481 tgcttgcgcc agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg
     3541 gcttccatca ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag
     3601 aatatctcga atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc
     3661 ataaatcggt agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga
     3721 accaccagcc agaataatac ctttacgcgt tttcatttca ctattccttt taattcatct
     3781 cgctctggca tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt
     3841 cgttgagcat acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct
     3901 gaaatttttc ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg
     3961 ttgtcggcac ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt
     4021 caaataccag agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca
     4081 aaccagcaac ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca
     4141 gtaattcagc ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt
     4201 cttttgccag gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc
     4261 tggtacggaa aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct
     4321 tggtttcacc gtagacattt agcggtgcgg ttgcatccgt ttccagccat ggcgtgtcac
     4381 cgttgcctgg gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag
     4441 ctgcttttgc aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg
     4501 attccgcttt gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt
     4561 ttttgacggt ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt
     4621 gaacatcgag cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca
     4681 cctgccctgt tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc
     4741 aatccacgac tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata
     4801 ccattccacc gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc
     4861 gcggctaatt ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac
     4921 ataagtaatt tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca
     4981 aatagtgagc actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc
     5041 acccgcttta ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa
     5101 ccagtcgcga atttgatccc ctttaccata aataggtaat gccttacctt ccagagcatt
     5161 cagaataacc aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca
     5221 attagtcaca atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct
     5281 ggaagcttta gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag
     5341 cggtaacgct tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc
     5401 agtagaaata tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta
     5461 attgcgcgcc gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc
     5521 agggcccgtt attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg
     5581 ctggtgctgt gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa
     5641 agcataacgt tcagaatcag aaacatcagc gagtgattct aggttcccgg cgtatgttaa
     5701 tttatcgaca ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga
     5761 accaataaac cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat
     5821 agagcatgct gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta
     5881 ccgcccctgg cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt
     5941 agccactcgc gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga
     6001 aacaaggaaa atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata
     6061 ggctttataa aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct
     6121 tcactgccgt tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct
     6181 taacagcttc tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata
     6241 cttcacaaac gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat
     6301 cagcattgca tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat
     6361 acgtccccat gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac
     6421 atagcgacct acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat
     6481 aaattcaaca atacggctga ctttaccctc acggtccagc ggctctttag tctggatgac
     6541 ggagtattca gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc
     6601 gttgaaacgt gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc
     6661 gatcacaacg tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca
     6721 caaaatggag tggcctaaac ctaaaggttc gccctgacgc acgttcataa tggtcacgcc
     6781 cggcggacag atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag
     6841 tgactctaac tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt
     6901 taccaggagg atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat
     6961 tggcttgtcg acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg
     7021 catcccgaga cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg
     7081 taaaatggtt gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa
     7141 ttgattactg aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt
     7201 cctaagtatg gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa
     7261 tctaaagtta atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga
     7321 gctttgccct ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt
     7381 cgccataaag acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc
     7441 aactgaggat tcctgcatca cattgatatt tcgtataaaa agatgttgcg gcttgttgtg
     7501 taactccagc gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt
     7561 cccggcggag atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat
     7621 attattcact cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc
     7681 gattaacatc ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc
     7741 gtaaatagcg actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt
     7801 gcgggcattg atattacgaa taacaaaatg tttaccattt tcaacatggt tcaactgccg
     7861 acaatccgat cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta
     7921 gttgttatca taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt
     7981 ggtacagttg atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc
     8041 cacgttccat tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc
     8101 accgataatc tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt
     8161 gaggttatcg atggtcaggt tgcgcatgac ccgtttgttt ttaccgccaa tataaatctg
     8221 cattaccggg ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aaccacgcac
     8281 atccagggta atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg
     8341 taagacaaat cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc
     8401 gggagggatg aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac
     8461 cgtttgccct tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc
     8521 gttaatgtta acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc
     8581 aagcgccgag cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc
     8641 tcctctataa agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat
     8701 cgtgttcgac tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt
     8761 ggctaaacgc cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc accagccagc
     8821 cggatttatc ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa
     8881 taccgaccgc catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc
     8941 ccgttaccga tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc
     9001 tcggtttaaa gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc
     9061 gcaggcgtcg ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct
     9121 ctttcaactg acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg
     9181 cgacagatat gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg
     9241 tcatgtccac gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca
     9301 gccttccggc ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt
     9361 gatattcggg agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga
     9421 tggtggcaat tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg
     9481 ccgggccaaa atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa
     9541 tggcagacaa aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc
     9601 aggtattttt acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta
     9661 ctttgcccgc aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc
     9721 atgccgcgtg ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc
     9781 ccatatcaat aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt
     9841 tcaataagaa gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac
     9901 catctgcatt cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac
     9961 cggaagctgg cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg
    10021 gatatcaatc gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc
    10081 atagttgatg gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg
    10141 cgtaccgacg gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc
    10201 atccatcact acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac
    10261 catgcggtcg tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg
    10321 atagccttca tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg
    10381 agtggtgccg agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt
    10441 ctgttgtgcg gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt
    10501 gtggtgatcg accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt
    10561 gctgcgtttc atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc
    10621 aaagacatag ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc
    10681 aatcataaac agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac
    10741 gccgtagaga tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag
    10801 caggcgcacg aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc
    10861 ggtgacgcgt gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaacgcg
    10921 cccgacaacg cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat
    10981 taccgggcgg ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc
    11041 ggtatgtgga ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc
    11101 acgattgccg caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg
    11161 atgccaggtg aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg
    11221 cgcgtcttat caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta
    11281 cgccgcatcc ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc
    11341 attttcattt tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt
    11401 gacagcacaa tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc
    11461 atccccagcg ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc
    11521 ggcaacgaga gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca
    11581 agaaccggtt taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg
    11641 aaaccgagcg tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata
    11701 aacagaaatg ttttgaatac attgaattta aagctgatat cgacccgcgc tttcgccatc
    11761 agcagcgaac caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc
    11821 agcaccggaa taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac
    11881 accaccatca gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg
    11941 tagaagttaa cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac
    12001 acgcgggtga tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg
    12061 ttgtatcccc ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg
    12121 gtgttgagat agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt
    12181 gccacagacg ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca
    12241 aacagcagtg ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc
    12301 ggccagaaat gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg
    12361 atcatgccga ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg
    12421 tgggggatca ccacaaacgc cagcgacaat gttttaatca acggtgccag gtccgggtta
    12481 ttcagcacgt cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc
    12541 agcccgacgt tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga
    12601 ataatcgagt tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc
    12661 gacacagtca gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc
    12721 accagcccga ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg
    12781 gttttttcac gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt
    12841 caggaaaacg attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa
    12901 ctcgacgcgt ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg
    12961 cgcccagccg gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg
    13021 gcgatactgt tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc
    13081 ggtcagcaca ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc
    13141 cactttagtg acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat
    13201 cactttcatg gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt
    13261 ctggcggaaa ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag
    13321 caccggggag atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag
    13381 caggcggtta ccccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat
    13441 ctcttcgaga cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga
    13501 acaggtggta tccgccagtt gatgggccag ttttttcact cgtgcgccgt cgcacatttg
    13561 catcgcgata tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag
    13621 gttacccgcc cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac
    13681 ttcaaaccct aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa
    13741 atcccccgct acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc
    13801 aatgcgaata catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag
    13861 ccagattttc agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat
    13921 cacgcttaag gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg
    13981 ccatgagcga taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag
    14041 cgccaccaac aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag
    14101 cccggcaaac atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt
    14161 ggttttcgct cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt
    14221 acccgcagtg gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg
    14281 cgttcgcgct tccatcagcg gcacatctcc gcgcgattcc acattcaggc gcaccaccgg
    14341 ttcggtattg gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc
    14401 gctgcgatcc accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc
    14461 aacgggttgc gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc
    14521 gcgcaccagt tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca
    14581 cgggatcatg ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc
    14641 gccaccgtag atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt
    14701 cgacattacc ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aggagagacg
    14761 cggatcatgg atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc
    14821 gacaatgtag tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa
    14881 atcgccatca aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc
    14941 gtcgcgacat tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt
    15001 gttgtgtact ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc
    15061 gtccaccacc ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag
    15121 attcttgaca ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg
    15181 ctgatagcga ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg
    15241 ctggacgtcg cgcagtccgg tatcaccgct gatcgggcga gccccctcgc gcaccagctt
    15301 catgccgttg taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc
    15361 gagatggaac gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc
    15421 gacgcccgca tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg
    15481 gacatcaccg cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat
    15541 gcgccaggcg atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt
    15601 aaagcaggtt aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga
    15661 aacggccaat tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc
    15721 gacgtttaca cccgtccgta gcgatccgcg aagcgcacca catcatcctc ttcgagataa
    15781 gagccggagc gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcagtgc
    15841 gtcgccccca gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca
    15901 atggtgactt ttgccgttcc cgcgaccact acccagtgtt ccgcgcggtg atggtgcatc
    15961 tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc
    16021 gcgtcgatag agtcatattt gccccacgga cgatacactt cgcgatgcac ccgatgctca
    16081 tggcgaccat cggctttgat ctgctcgacc acttttttca catcctgtac cgcgttacgg
    16141 tcggcaatca gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg
    16201 gtgactaggc ctgattcggc atacacatag ctgttttcag ttttgtgatt aatcacatcg
    16261 ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct cccataatga agaccaggaa
    16321 ccaacatcgc tccagcccgc atccatcggc accacaacag catctgccgt acgttccatg
    16381 accgcgtaat ccaccgactc ttccgggcag gcgagaaacg cttcttcatc tacacgaata
    16441 aaatcgagat ccggatcgac ggcgctcatc gctttttcgc aggcatcgag gatatccggg
    16501 cgatattttt tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc
    16561 cagtaatatt cgccgcttgc tacataggcc tgggcggttt ccagattcgg tttttcgaca
    16621 aactgcgcca cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga
    16681 atatagccat aaccggtttc tggtagatcc ggcacaatgc caaaggtcac cagcttgccc
    16741 gcttcggcat aaggcatggc attacgcacg gcggcacgga acgcgtcttt atcggcaatc
    16801 acatgatccg ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc
    16861 gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc ctgccggttc gagaataatg
    16921 ttctcggtga gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca
    16981 ttgcaaatca ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc
    17041 agcatggtga gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc
    17101 ggccataaac ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc
    17161 ataattatcc ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagcgtgc
    17221 gttcggcata ttcacgtgcc accgtgttat gtttgggcag caggagcgcc tgacgaatcc
    17281 ccgccaccag cgcctcgacc gattccggtt ctacgcaaac cgcaataccc ggaaaggttt
    17341 cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg
    17401 ccagaatatt ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttacacca
    17461 ccagatggca atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga
    17521 aaaattgcat gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc
    17581 cgccctgccc gacaatggca aaaagcagcg gttcatcgcg caggagatcg gcagcttcaa
    17641 taacgttttc cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt
    17701 tatccggcag gccaagctgg ttacgaaggg catcaacatc ggcgtctgca acatgctgaa
    17761 aacgggcaat ttccgaccag ttggggaaga agatgacgtt atccgccgcc acgccttttt
    17821 cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac gttatcgacg ttatgcagtc
    17881 cgctacgttc gaacgccgtt gccagctgtg ccactttgcc gcctttgcct tttccggcaa
    17941 ggcccagccc cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac
    18001 cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc actacgccaa
    18061 taatgcgatc cggcttccag cgacgttgcg ccatcagtgg aaagaaactg ctgacggcaa
    18121 aactgccgag atgcaacaag cgtttcaggg tgctcggctg ttttggcacg tacagcgggc
    18181 agcgccacac cgtggcggcc ccttcttctc gtttgtagcg ccaggcggaa tagttctcac
    18241 ccacctgcca ttgcgggtag taaggcggtg cggtaatgac ccgcacctca tgaccttgtg
    18301 ccgccaacca ttccaccatc tcgccggtgt atttgccgat gccggttaac tccggcgagt
    18361 agttaatgcc gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag
    18421 aaaataggcg cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag
    18481 ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc tcttcttccg ctactctgaa
    18541 gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata
    18601 aaaatgctgc cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc
    18661 cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg tctttctgca cgcgccctcc
    18721 cggcacaaac cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcgcc
    18781 gcgactgttc tcgacaataa agtcgagaga gacaagcggt gtggagcgca ctacagtggc
    18841 aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc ggtcttgatt ctcaaggaac
    18901 cactggtaag tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga
    18961 tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa
    19021 accacccgac ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg
    19081 cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg gttggctatt ctccagccag
    19141 acttcatgcg ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg
    19201 aattcacgca tcggtgtacc gctgccccat accaccacgt ccggcgcatt ctgtgccgtc
    19261 gcctcgtgga agcgacgcag caatgctggt atcacatggg agttactcgg gtggaagttg
    19321 tcatgcggcc cgtacaggtt ggtcggcatg acagagcggt aatcgcgtcc atactggcgg
    19381 ttgtaagatt cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc
    19441 ggctccagcg tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg
    19501 tagatgcagg acgatccgag aaacatcagt ttgttcacgt cgttctgatg cgcggcgtga
    19561 atgatgttgc tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg
    19621 gcaacaatgc cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca
    19681 aagaaatcat gcacggcgcg gctgtccagc aggttcagtt catcgcgggt acgtaatacc
    19741 agctccacat caccgcgctg ttcgagctgc cgcttgatgg cagaaccgac catcccacaa
    19801 tggccagcga taaaaatgcg ttgtttactt atgcttatga ctccagcgcg atcgccacgt
    19861 cgtagccgtg agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca
    19921 tttcagacac catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt
    19981 tggtcgggtc gccgagcagc gtttcaactt ctgccggacg gaagtaacgc ggatcaacgg
    20041 cgataatcac atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa
    20101 tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgctgccg
    20161 ccatttccac aaactgacgt acggagtact gaacgccggt cgcgataacg aaatcttccg
    20221 gctgttcctg ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt
    20281 cacgcaggga atccatattt ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg
    20341 cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt
    20401 tgaagagaat tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc
    20461 agtaggcgta cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt
    20521 tctgcggaat ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag
    20581 ttttcttttc cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt
    20641 cgacgtcagc ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca
    20701 ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac
    20761 tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt
    20821 gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac
    20881 ctttttccag cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga
    20941 gagcgacttt tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt
    21001 tcgcgtatca ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa
    21061 acactgcttc gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag
    21121 acatcggttg ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa
    21181 tgttgacttg catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa
    21241 tgtgcgccaa tggttatttc accgagggta tataaattga cgtcatcgcc aacccacgcg
    21301 taatcaccta aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg
    21361 ttttttccta tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact
    21421 tgtggcgacc aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg
    21481 ttgccgcccc ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct
    21541 tatattcaga ctttgttata taaggcttta gtcttacctg tcgtacgttg gcgtaaatgc
    21601 caggataatt ctgcccagaa gccaggtaca tgtaatatct gccgttggac ttttttggcg
    21661 tcagcac
//
LOCUS       DADLXX010000060        21644 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLXX010000060 DADLXX010000000
VERSION     DADLXX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.060"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..157
                     /locus_tag="JNX27_002224"
     CDS             <1..157
                     /locus_tag="JNX27_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7883429.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JNX27_002225"
     CDS             227..1033
                     /locus_tag="JNX27_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7883430.1"
                     /translation="MQTWLNLQDKIIIVTGSASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JNX27_002226"
     CDS             1043..1450
                     /locus_tag="JNX27_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7883431.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JNX27_002227"
     CDS             1450..1944
                     /locus_tag="JNX27_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY7883432.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JNX27_002228"
     CDS             2010..2807
                     /locus_tag="JNX27_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7883433.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JNX27_002229"
     CDS             2818..3606
                     /locus_tag="JNX27_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY7883434.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /locus_tag="JNX27_002230"
     CDS             3658..4887
                     /locus_tag="JNX27_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7883435.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JNX27_002231"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JNX27_002231"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7883436.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JNX27_002232"
     CDS             complement(6049..7680)
                     /locus_tag="JNX27_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7883437.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNX27_002233"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNX27_002233"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7883438.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JNX27_002234"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JNX27_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7883439.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /locus_tag="JNX27_002235"
                     /pseudo
     CDS             12924..15108
                     /locus_tag="JNX27_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNX27_002236"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNX27_002236"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY7883440.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNX27_002237"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNX27_002237"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY7883441.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNX27_002238"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNX27_002238"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY7883442.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNX27_002239"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNX27_002239"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7883443.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21577
                     /locus_tag="JNX27_002240"
     CDS             21287..21577
                     /locus_tag="JNX27_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883444.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5339 a         5549 c         5469 g         5287 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcagcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccag atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttactg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaatggaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ccttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac ttgctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagg cggttgggtt
    21481 ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc
    21541 gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca
    21601 agctggagaa gacgcgccag cgtcgcaccc ggcatttttt tcac
//
LOCUS       DADLXX010000061        21504 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLXX010000061 DADLXX010000000
VERSION     DADLXX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.061"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNX27_002241"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNX27_002241"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7883445.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNX27_002242"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNX27_002242"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7883446.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEMEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JNX27_002243"
     CDS             complement(3121..3420)
                     /locus_tag="JNX27_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883447.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JNX27_002244"
     CDS             3595..4590
                     /locus_tag="JNX27_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7883448.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JNX27_002245"
     CDS             complement(4632..5069)
                     /locus_tag="JNX27_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7883449.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JNX27_002246"
     CDS             complement(5115..5456)
                     /locus_tag="JNX27_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883450.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNX27_002247"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNX27_002247"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7883451.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMLALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNX27_002248"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNX27_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="JNX27_002249"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="JNX27_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7883452.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     TKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEKEL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="JNX27_002250"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="JNX27_002250"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY7883453.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="JNX27_002251"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="JNX27_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7883454.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13864
                     /locus_tag="JNX27_002252"
                     /pseudo
     CDS             12686..13864
                     /locus_tag="JNX27_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13972..14796)
                     /locus_tag="JNX27_002253"
     CDS             complement(13972..14796)
                     /locus_tag="JNX27_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7883455.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="JNX27_002254"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="JNX27_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="JNX27_002255"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="JNX27_002255"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7883456.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="JNX27_002256"
     CDS             complement(18728..19201)
                     /locus_tag="JNX27_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7883457.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNX27_002257"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNX27_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7883458.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20351..21349
                     /gene="yiaK"
                     /locus_tag="JNX27_002258"
     CDS             20351..21349
                     /gene="yiaK"
                     /locus_tag="JNX27_002258"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7883459.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21361..>21504
                     /locus_tag="JNX27_002259"
     CDS             21361..>21504
                     /locus_tag="JNX27_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY7883460.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5406 a         5488 c         5277 g         5333 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccatttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcga
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacacc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagaa agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctcgacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc agcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgtcgat
    20881 gttctcttac ggcatgttag aagttaaccg cctggcaggc cgtcagctcc cggtcgatgg
    20941 tggctttgat gatgagggca atttgaccaa agaacctggc gttatcgaga agaatcgccg
    21001 cattttgccg atgggctact ggaaaggttc tggcatgtcg attgtgctgg atatgatcgc
    21061 tactctcctt tccgacggcg catcggttgc cgaagtcacc caggacaaca gcgacgaata
    21121 cggcatttca caaattttta ttgccattga agtggacaag cttatcgacg gtcccacccg
    21181 cgatgccaag ctgcaacgta tcatggatta cgttactacc gctgaacgcg ctgacgaaaa
    21241 ccaggccatc cgcttacccg gccacgaatt tactaccctg ctggccgaaa accgccgtaa
    21301 cggcatcact gttgatgaca gcgtgtgggc caaaatccag gcgttatgag gagataaatc
    21361 atgatttttg gacatatcgc gcagcccaat ccgtgccgtt tgcccgccgc cattgaaaag
    21421 gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg agccgggcgt tgtcgaaatc
    21481 gacggcaaga atatttatgc gcaa
//
LOCUS       DADLXX010000062        21417 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLXX010000062 DADLXX010000000
VERSION     DADLXX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.062"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..2005
                     /gene="speF"
                     /locus_tag="JNX27_002260"
                     /pseudo
     CDS             1..2005
                     /gene="speF"
                     /locus_tag="JNX27_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="JNX27_002261"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="JNX27_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY7883461.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGTSLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNX27_002262"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNX27_002262"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7883462.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNX27_002263"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNX27_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7883463.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="JNX27_002264"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="JNX27_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7883464.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6608..6970
                     /gene="ybfE"
                     /locus_tag="JNX27_002265"
     CDS             6608..6970
                     /gene="ybfE"
                     /locus_tag="JNX27_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7883465.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="JNX27_002266"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="JNX27_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7883466.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="JNX27_002267"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="JNX27_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7883467.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="JNX27_002268"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="JNX27_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7883468.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="JNX27_002269"
     CDS             complement(8459..8785)
                     /locus_tag="JNX27_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883469.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNX27_002270"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNX27_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY7883470.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="JNX27_002271"
     CDS             complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="JNX27_002271"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7883471.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="JNX27_002272"
     CDS             complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="JNX27_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7883472.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14952..15752
                     /gene="nagB"
                     /locus_tag="JNX27_002273"
     CDS             14952..15752
                     /gene="nagB"
                     /locus_tag="JNX27_002273"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7883473.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15812..16960
                     /gene="nagA"
                     /locus_tag="JNX27_002274"
     CDS             15812..16960
                     /gene="nagA"
                     /locus_tag="JNX27_002274"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7883474.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAGAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            16969..18189
                     /gene="nagC"
                     /locus_tag="JNX27_002275"
                     /gene_synonym="nagR"
     CDS             16969..18189
                     /gene="nagC"
                     /locus_tag="JNX27_002275"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7883475.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18237..18979
                     /gene="nagD"
                     /locus_tag="JNX27_002276"
                     /pseudo
     CDS             18237..18979
                     /gene="nagD"
                     /locus_tag="JNX27_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19376..21040
                     /gene="asnB"
                     /locus_tag="JNX27_002277"
     CDS             19376..21040
                     /gene="asnB"
                     /locus_tag="JNX27_002277"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7883476.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYARDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         5262 a         5430 c         5401 g         5324 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatt agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggca cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcttg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cttgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga actgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctgacagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10321 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    10381 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    10441 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    10501 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    10561 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc
    10621 tatttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa
    10681 tatattgcag agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga
    10741 aatttattgt ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact
    10801 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
    10861 ggtttttcgg ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg
    10921 aatgctttac ccgctaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
    10981 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg
    11041 aacagacgat catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
    11101 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa
    11161 gtacagaaga tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg
    11221 acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
    11281 gcttcttcgc ggaaatcggc gcgatcgatc caaatttcac cgctaaacgg cacctgacgg
    11341 ctccccattt ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga
    11401 tagttttcga taaccagttt caccggatcg ataactgcca ttgcgcgcgg cgcattttcg
    11461 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
    11521 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
    11581 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
    11641 agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
    11701 tactggcgcg gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg
    11761 ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg
    11821 caatgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
    11881 gcaaatttaa tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg
    11941 attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac
    12001 agcgccaagt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc
    12061 agggtaccgc ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct
    12121 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
    12181 acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac
    12241 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
    12301 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
    12361 ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca
    12421 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
    12481 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
    12541 ttagtttttt aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag
    12601 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag
    12661 gcatacagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    12721 ctgagcttta atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
    12781 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    12841 gcttacctgc gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    12901 gtcgataccc atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg
    12961 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    13021 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    13081 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    13141 tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc
    13201 agcggctacc ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
    13261 cgcgccaaca atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag
    13321 acgtttacac atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt
    13381 aatacaggca tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    13441 tgccagttga gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc
    13501 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
    13561 cacgaagtag atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct
    13621 ggcggccggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    13681 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    13741 gaggtacagc agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    13801 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    13861 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    13921 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    13981 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    14041 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    14101 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    14161 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    14221 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    14281 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    14341 ttctgggttg atgatcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    14401 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    14461 tgcgaggtta tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    14521 tggctgaccg aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa
    14581 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    14641 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct
    14701 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa
    14761 ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    14821 tggctaaacc agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct
    14881 gcgcaaatcc aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat
    14941 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    15001 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac
    15061 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    15121 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    15181 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    15241 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg
    15301 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    15361 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    15421 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
    15481 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
    15541 aagaagtgat gattctggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg
    15601 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca
    15661 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    15721 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ttccttgctc
    15781 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
    15841 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    15901 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    15961 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    16021 caccgctgga gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    16081 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    16141 cgtgcgcgtt atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
    16201 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    16261 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    16321 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    16381 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg
    16441 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
    16501 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    16561 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    16621 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    16681 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    16741 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    16801 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg
    16861 cacgctcgcc gcaggtaaag tagccaacct gaccgcattc acacctaatt ttaaaatcac
    16921 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    16981 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    17041 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    17101 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    17161 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    17221 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    17281 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    17341 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaagctg
    17401 cgcgagctaa tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    17461 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actgaaagaa
    17521 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    17581 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    17641 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa
    17701 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    17761 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    17821 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    17881 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa
    17941 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc
    18001 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg
    18061 aaggcgtttc gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    18121 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    18181 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    18241 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
    18301 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    18361 ccaactatcc ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
    18421 atgtgccgga tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
    18481 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    18541 ccggtttcac tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttcct
    18601 acaactggga catgatgcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg
    18661 ccaccaaccc ggacacggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa
    18721 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcggc
    18781 attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata acctgcgtac
    18841 cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt ctggtgtttc
    18901 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc
    18961 tgaaatcgac gttatctgaa taccccccac gtctgcggac gtggttggct ctgatgacaa
    19021 acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga
    19081 attttcatgc ttttgtaggc cgtataaggc gttcacgccg catccggcat gaacaaatcg
    19141 cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc cgctaaaaat
    19201 tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat cgacaatcac
    19261 cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc tttgcggcat
    19321 ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg
    19381 ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact
    19441 cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgcccgcga
    19501 taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc
    19561 tctctacaac caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct acaaccacca
    19621 ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt
    19681 gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt
    19741 tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg
    19801 gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg cctcagaaat
    19861 gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg
    19921 gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact ttgatgcagt
    19981 gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt ccgttaaaag
    20041 ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg attcctcaat
    20101 tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga
    20161 agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatcttaa
    20221 agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc acttcactgt
    20281 acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt atgatgtgac
    20341 cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat
    20401 taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc tttacttcca
    20461 taaagcgccc aacgctaaag aactacatga agagacggtg cgtaaactgc tggccctgca
    20521 tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt
    20581 tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc aggataaaat
    20641 gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt catacctgcc
    20701 cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat
    20761 cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg aaactgcccg
    20821 cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc gcgagatctt
    20881 tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc cttccgtcgc
    20941 gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg acgatccgtc
    21001 tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt actgccccgg
    21061 agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg cagtaaggaa
    21121 atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata
    21181 gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac aaaagaatgg
    21241 ctgtagggtg gggaaaaaac acaaagtaaa caataattga cgaatatagc gccacgctgt
    21301 tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag gttgacgctg
    21361 caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga aaaaaag
//
LOCUS       DADLXX010000063        21400 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLXX010000063 DADLXX010000000
VERSION     DADLXX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.063"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="JNX27_002278"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="JNX27_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7883477.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="JNX27_002279"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="JNX27_002279"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7883478.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JNX27_002280"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JNX27_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7883479.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JNX27_002281"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JNX27_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7883480.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JNX27_002282"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JNX27_002282"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7883481.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="JNX27_002283"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="JNX27_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7883482.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11351
                     /gene="ftsK"
                     /locus_tag="JNX27_002284"
     CDS             7245..11351
                     /gene="ftsK"
                     /locus_tag="JNX27_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7883483.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAVDPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11506..12117
                     /gene="lolA"
                     /locus_tag="JNX27_002285"
     CDS             11506..12117
                     /gene="lolA"
                     /locus_tag="JNX27_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7883484.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDEQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12128..13471
                     /locus_tag="JNX27_002286"
     CDS             12128..13471
                     /locus_tag="JNX27_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7883485.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13562..14854
                     /gene="serS"
                     /locus_tag="JNX27_002287"
     CDS             13562..14854
                     /gene="serS"
                     /locus_tag="JNX27_002287"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7883486.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15094..17538
                     /gene="dmsA"
                     /locus_tag="JNX27_002288"
                     /pseudo
     CDS             15094..17538
                     /gene="dmsA"
                     /locus_tag="JNX27_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            17549..18166
                     /gene="dmsB"
                     /locus_tag="JNX27_002289"
     CDS             17549..18166
                     /gene="dmsB"
                     /locus_tag="JNX27_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7883487.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18168..19031
                     /locus_tag="JNX27_002290"
     CDS             18168..19031
                     /locus_tag="JNX27_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY7883488.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19067..19693)
                     /locus_tag="JNX27_002291"
     CDS             complement(19067..19693)
                     /locus_tag="JNX27_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7883489.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20008..21156
                     /locus_tag="JNX27_002292"
     CDS             20008..21156
                     /locus_tag="JNX27_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883490.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGSVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         5281 a         5376 c         5660 g         5083 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat
      121 atctacatcg agacaagtta tggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tttgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcac aaaagacaaa cagggcaatt aatgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata acgctgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat tttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cacgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcggccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacctgca gccagcgtca ccccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta gcggtgctgt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca atacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg cgccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgta tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtct agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgtcgaccc ggacgacgtc ctatttttgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgcaa ctgcgccgga acagcctgcg caacagccgt
     8581 attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc
     8641 aatccatttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
     8701 ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg
     8761 aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg
     8821 aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac
     8881 cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac
     8941 ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
     9001 tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg
     9061 gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
     9121 gcgtgccaac tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca cagcgtgcgg
     9181 cggaagaaaa agcccgtgaa gcccagcgca atcagtatga ttctggcgat cagtataacg
     9241 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
     9301 agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg
     9361 ctgcggcaga ggctgaactg gctcgtcagt ttacgcaaac tcaacaacaa cgttattccg
     9421 gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga
     9481 aagcgttgct ggatgatggt ccacacgaac cgctgtttac gccaattgtt gaacctgtac
     9541 agcagccgca acaaccggtt gcaccacagc agcagtatca gcagccacaa cagccggttg
     9601 cgccacagcc gcagtatcag cagccacaac agcaggttgc gccgcagcca caatatcagc
     9661 agccgcaaca accggttgcg ccacagcagc aatatcagca gccgcaacaa ccagttgcgc
     9721 cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc
     9781 cacaacagcc agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta
     9841 atggcgacag ccgtccgttg cacaaaccga cgacgccgct gccttctctg gatttgctga
     9901 caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggcgcgtc
     9961 tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgttaat tactctccgg
    10021 ggccagttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt
    10081 ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag
    10141 ttattcctgg caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt
    10201 atctgcgcga agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg
    10261 tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact
    10321 tgttggttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga
    10381 gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc
    10441 tggagctttc ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga
    10501 aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga
    10561 tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc
    10621 gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc
    10681 atccggtgct gaaaaaagaa ccatacattg tcgtgttggt tgacgaattt gccgacctga
    10741 tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg
    10801 ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc
    10861 tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac
    10921 gtaccattct tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact
    10981 ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag
    11041 ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca
    11101 cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc
    11161 cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg
    11221 tacagcgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag
    11281 cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc
    11341 cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat
    11401 caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag
    11461 tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgct
    11521 atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc
    11581 gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg
    11641 actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca
    11701 aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa
    11761 acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc
    11821 accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac
    11881 aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg
    11941 aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag
    12001 caggacgagc agcgcagcag ttatcagctg aaatcccagc aaaatggggc tgtggatgca
    12061 gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg
    12121 cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc
    12181 gcgtatgcgg ccagaaaatt tagcacagta tatcggtcag caacatttgc tggctgcggg
    12241 gaagccgttg ccgcgcgcta tcgaagccgg gcatttgcat tctatgatcc tctgggggcc
    12301 accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt
    12361 ggaacgtatt tctgccgtca cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc
    12421 ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt
    12481 caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat
    12541 tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg
    12601 tgtttatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat
    12661 ggaagacaaa acccgtggct atggtggtca ggatattgtt ctgccagatg atacacgacg
    12721 tgccattgca gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat
    12781 ggcggatatg gccgaagtct atgatagcgg taagcgggtc ctgaagcctg aattactgac
    12841 cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct
    12901 aatttccgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta
    12961 tgcgcgaatt attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat
    13021 tgcgtctgaa gacgtcggta atgccgatcc acgggcgatg caggtggcaa ttgcggcctg
    13081 ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt
    13141 ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc
    13201 cgatgctcgc gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa
    13261 attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc
    13321 ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc
    13381 gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga
    13441 tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt
    13501 atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag
    13561 catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg
    13621 ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt
    13681 gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca
    13741 ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga
    13801 agagctggat gcagcaaaag ccgagctaga tgctttacag gctgaaattc gcgatatcgc
    13861 gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa
    13921 cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt
    13981 gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc
    14041 ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat
    14101 gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa
    14161 ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac
    14221 tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt
    14281 tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat
    14341 gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg
    14401 tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccagaaga
    14461 ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg
    14521 cctgccgtac cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac
    14581 ttacgacctg gaagtatgga tcccggcaca gaacacctac cgtgagatct cttcctgctc
    14641 caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgccgcagca agtcggacaa
    14701 gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt
    14761 tgcagtaatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg
    14821 tccgtatatg aacggactgg aatatattgg ctaataccca atttttctga atctaaaaag
    14881 cgcctttagg gcgctttttt tgtctccctt tgataccgaa caataattac tcctcactta
    14941 cacgtaatac tactttcgag tgaaaatcta cctatctctt tgattttcaa attattcgat
    15001 gtatacaagc ctatatagcg agctgctata gaaataatta cacaatacgg tttgttactg
    15061 gaatcaatcg tgatcaagct tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta
    15121 ttggctgctg aggagagtcg ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca
    15181 atggccagca gcgcattaac attacctttt agtcggattg cgcacgctgt cgatagcgcc
    15241 attccaacaa aatcagacga aaaggttatc tggagcgcct gtacagttaa ctgtggtagt
    15301 cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca aatatgtcga aacggacaat
    15361 accggcgatg acaattacga cggcctgcac taggttcgcg cctgcctgcg tgggcgttcc
    15421 atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc cgatgaaacg agtcggggcg
    15481 cgcggtgaag gcaaattcga gcgcattagc tgggaagaag cctacgacat catcgcgacc
    15541 aatatgcagc gcctgatcaa agagtacggc aacgagtcta tctatctgaa ctatggcacc
    15601 ggtacgctgg gcggcaccat gacccgctcc tggccgccgg gaaataccct ggtcgcgcgc
    15661 ctgatgaact gctgcggcgg ttatcttaac cattacggtg actactcttc cgcgcaaatt
    15721 gcggaaggtt tgaaccatac ctacggcggc tgggcagatg gtaacagccc gtcggatatc
    15781 gaaaacagta aattggtagt gctgtttggt aataacccag gcgaaacgcg aatgagtggc
    15841 ggtggggtga cttactatct tgaacaggca cgccagaaat ctaatgctcg cataatcatc
    15901 atcgatccgc gctataccga caccggtgcc gggcgcgaag atgagtggat ccctattcgt
    15961 ccaggaacag atgccgcact ggttaacggt ctggcgtacg tcatgatcac tgaaaacctg
    16021 gtggatcagg cattcctcga taaatattgc gttggctatg acgagaaaac cctgcccgcc
    16081 agtgcgccga aaaatggtca ttataaagct tatattctgg gcgaagggcc agatggcgtg
    16141 gctaaaacac cggaataggc ctcgcaaatc accggtgttc cggcagacaa aatcatcaaa
    16201 ctggctcgtg aaatcggcag taccaaaccg gcgtttatca gccagggatg gggcccgcag
    16261 cgtcacgcta acggtgaaat cgcaacccgt gctatctcga tgctggcgat tctgaccggt
    16321 aacgttggta ttaatggagg gaacagcggc gcgcgtgaag gttcatacag cttaccgttt
    16381 gtccgtatgc cgaccttgga aaacccgatc cagaccagca tttcgatgtt tatgtggacc
    16441 gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg atggtgtgcg cgggaaagat
    16501 aagctggatg tgccgatcaa aatgatctgg aactatgccg gtaactgcct gattaaccag
    16561 cattctgaaa tcaaccgtac ccatgaaatc ctccaggatg ataagaagtg cgagctgatt
    16621 gtggttatcg actgccacat gacctcatcg gcgaaatatg ctgacatcct gctgcctgac
    16681 tgcaccgctt ccgaacagat ggactttgca ctggatgcat cctgcgggaa tatgtcttac
    16741 gtgattttca acgatcaggt gattaaaccg cgctttgaat gtaagaccat ctatgaaatg
    16801 accagcgaac tggcaaaacg tcttggcgtt gagcaacagt ttactgaagg ccgtacccag
    16861 gaagagtgga tgcggcatct gtatgcccag tcgcgggaag cgattcctga actgccaacg
    16921 tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg acccacaagg gcatcacgtt
    16981 gcttataaag ccttccgtga agatccgcag gcaaacccac tgactacgcc atcgggcaaa
    17041 attgagattt attcgcaggc gctggctgac attgccgcta cctgggaatt gccagaaggc
    17101 gatgtgatcg atccactgcc gatctacacg ccgggctttg aaagttatca ggatccgctg
    17161 aacaaacagt atccgctgca gcttacaggt ttccactata aatctcgcgt tcactcaact
    17221 tacggcaacg ttgatgtgct gaaagcggct tgccgtcagg aaatgtggat caacccgctt
    17281 gatgcccaaa aacgcggtat ccacaacggc gataaagtca ggatctttaa cgatcgtggt
    17341 gaggttcata ttgaggcgaa agtgacgcca cgaatgatgc cgggcgtggt cgcactgggt
    17401 gaaggcgcct ggtatgaccc ggatgcaaaa cgtgtcgata agggcggttg tatcaacgta
    17461 ctgaccactc aacgtccgtc tcctctcgct aaggggaatc cgtcacatac aaaccttgtt
    17521 caggttgaaa aggtgtaagg agtaaccgat gacaacccag tatggatttt ttattgattc
    17581 cagccgttgc accggttgca aaacctgcga gctggcttgt aaagactaca aagatttgac
    17641 gccagaagtc agtttccgcc gcatttatga atatgctggc ggcgactggc aggaagataa
    17701 cggtgtctgg caccagaacg tgtttgccta ctatctgtcg atttcgtgca accactgcga
    17761 agatccggct tgtactaaag tctgcccgag cggtgcgatg cataaacgcg aagatggttt
    17821 tgttgtggtc gatgaagatg tatgcattgg ctgccgttac tgtcatatgg cttgcccgta
    17881 tggcgcaccg caatataacg aaacgaaagg ccatatgacc aaatgcgatg gttgttatga
    17941 ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct tgcccgctgc gggcgctgga
    18001 tttcggccct atcgacgagc tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt
    18061 gccgcgagct cactttacca aaccgaatat tgtgatcaaa cccaatgcca atagccgccc
    18121 gacaggggat accactggct atctggcaaa cccgaaggag gtgtgagatg ggaagtggat
    18181 ggcatgaatg gccgctgatg atcttcacgg tcttcgggca atgtgtggcg ggtggtttta
    18241 tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta
    18301 tcgcctgcat gtttggctta tgggtactga tgggcattgg ctttatcgcc tctatgctcc
    18361 atcttggttc accgatgcgc gcctttaact cgctcaaccg ggtaggggct tcagcactca
    18421 gtaacgaaat cgccagcggt tcgatctttt ttgctgtagg cggcatcggc tggttgctgg
    18481 caatgctgaa aaagctgtca ccggcattgc gtacgctatg gctggtggtg acgatggttc
    18541 ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa cagcattgat accgttccga
    18601 cctggtacag catctggacg ccgatgggct tcttcctgac gatgttcatg ggcggcccgt
    18661 tactgggtta cctgttgctg agtctggctg gcgtcgatgg ctgggcgatg cgtctgctgc
    18721 cagccatttc tgtactggca ctggtggtga gtggcgtgat gtcagtgatg cagggcacag
    18781 agctggcaac tattcatagt tctgtgcagc aggccgcagc gctggtgccg gactatggtg
    18841 cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac
    18901 agctaaaagg ttatcagcct gcggttccgc tactatccgt ctcattcatt ctgcttctgg
    18961 caggggaatt aatcggtcgc ggcgtattct atggtttgca tatgaccgtg gggatggccg
    19021 tcgcaagcta atcagtaaca accggggttt cggccccggt tctcttttat ttctgcttcg
    19081 ttaacgtgtc ataactggtc atcaaattac gataatcagg aatatggtta gagaacaacg
    19141 tcgccagtcc ttcaatatca ttacgccagt cacggtgcag ctcgcaagcc acgccgaacc
    19201 atgtcatcaa ttgcgcacca gcttgcgaca tgcgatccca cgccgaatgc cgggtaattt
    19261 cattaaaggt ccctgatgca tcagtcacga caaagacatc aaacccttct tcaatggctg
    19321 aaagcgccgg gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt
    19381 tacctgtcgc tttgactgct tttacaaaat cttcgttatc ccaggcgtta atattgcccg
    19441 ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag ttctagaact aatgggccgt
    19501 taggaccggt ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagccaggt
    19561 cacccagcgc cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa
    19621 gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat
    19681 acggtttggt catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcggtcagac
    19741 gcgatgcatt gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt
    19801 ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat aaaaacaatc agatgagatt
    19861 aatcatctct tcagcattag cgttatgaat gagtaatata aatcgataat accgccgctg
    19921 cgatatgcgc gtattttcac ccattgacaa tgtttttggc ggtggcatga tgcgcatgaa
    19981 atttgaactt cctcacggtt ttaattcatg tccacgtata cccggcctgt catgcttttg
    20041 ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt taaatacact cgtgccgctt
    20101 tggctcgccc aggaacacat gtccacatgg caggtaggcg ttgtcagctc atcctatttt
    20161 accggcaacc ttgtcggtac attgctgaca gggtatgtca ttaaacgcat tggctttaac
    20221 cgcagctatt atctgtcctc cttcattttt gctgctggct gtgccggcct tggcctgatg
    20281 attggtttct ggagctggtt ggcgtggcgt tttgtcgcgg gcgtcggctg tgccatgatt
    20341 tgggtggttg ttgagagcgc gctgatgtgc agtgggacgt cacgtaaccg tgggcgtttg
    20401 cttgctgcgt atatgatggt ttattacgtg ggaacgtttt taggccagtt actggtcagc
    20461 aaagtctcaa ccgagctgat gagcgtattg ccgtgggtta caggtttgac attggcaggg
    20521 atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg ctgaaaacca tgattcgacg
    20581 tcaattacgg caatgctaaa actccgtcag gcgcggcttg gcgtgaatgg ctgcattatc
    20641 tcaggaagcg ttctgggatc tctatatggc ctgatgcctc tgtacctcaa tcacaaaggg
    20701 gtgagcaatg ccagtattgg tttctggatg gcggtactgg tcagtgcggg tatcctcgga
    20761 caatggccga ttggacgtct ggcggataag tttggtcgac tgctggtttt gcgtgttcag
    20821 gtctttgtcg tcattctcgg cagtattgcg atgcttagcc aggcggcgat ggccccagcg
    20881 ttattcatcc tcggtgccgc tggctttacg ctatatccgg tggcgatggc ctgggcttgt
    20941 gagaaagttg aacatcatca actggtggcg atgaaccaag ccttactgtt gagctatacc
    21001 gtgggaagtc tgcttggccc gtcatttacc gctatgctaa tgcagaattt ctccgataat
    21061 ttattgttta tcatgatcgc cagcgtatcg tttatctatt tgctgatgct gcttcgcaac
    21121 gccggtcata cgccgaaacc cgttgctcac gtgtaaatga attcaagcaa agtgtgaact
    21181 tactgtttca cactctgctt ttttgtttct tctatctgac ttgctttttt ccaaatttta
    21241 ttcgtttaaa aataaaatgt gcagcaggtt ataattttgc atttcgctat ttccgcactt
    21301 cttatttgcc gcgcataatc cctcgtttta cagatgcccc tttaattttg gcgaaggatt
    21361 tgtctatggc tgggaatgtt caggaagtaa gcgccccatc
//
LOCUS       DADLXX010000064        21264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLXX010000064 DADLXX010000000
VERSION     DADLXX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.064"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            15..923
                     /locus_tag="JNX27_002293"
     CDS             15..923
                     /locus_tag="JNX27_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883491.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTSQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLATLTSPLTSTTPVAENVE"
     gene            1162..1221
                     /locus_tag="JNX27_002294"
     CDS             1162..1221
                     /locus_tag="JNX27_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7883492.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1538..1597
                     /gene="ibsE"
                     /locus_tag="JNX27_002295"
     CDS             1538..1597
                     /gene="ibsE"
                     /locus_tag="JNX27_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7883493.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JNX27_002296"
     CDS             complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JNX27_002296"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7883494.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JNX27_002297"
     CDS             complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JNX27_002297"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7883495.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6061..7362)
                     /locus_tag="JNX27_002298"
     CDS             complement(6061..7362)
                     /locus_tag="JNX27_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7883496.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWLYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLIQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7604..8224
                     /locus_tag="JNX27_002299"
     CDS             7604..8224
                     /locus_tag="JNX27_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7883497.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPNRKRKDRWMN"
     gene            8288..9526
                     /gene="cca"
                     /locus_tag="JNX27_002300"
     CDS             8288..9526
                     /gene="cca"
                     /locus_tag="JNX27_002300"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7883498.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JNX27_002301"
     CDS             complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JNX27_002301"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7883499.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JNX27_002302"
     CDS             complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JNX27_002302"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7883500.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11074..11691
                     /gene="plsY"
                     /locus_tag="JNX27_002303"
     CDS             11074..11691
                     /gene="plsY"
                     /locus_tag="JNX27_002303"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7883501.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="JNX27_002304"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="JNX27_002304"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12842..13685
                     /gene="ttdA"
                     /locus_tag="JNX27_002305"
                     /pseudo
     CDS             12842..13685
                     /gene="ttdA"
                     /locus_tag="JNX27_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13682..14287
                     /gene="ttdB"
                     /locus_tag="JNX27_002306"
     CDS             13682..14287
                     /gene="ttdB"
                     /locus_tag="JNX27_002306"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7883502.1"
                     /translation="MKKILTTSIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14335..15798
                     /locus_tag="JNX27_002307"
     CDS             14335..15798
                     /locus_tag="JNX27_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7883503.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYPPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JNX27_002308"
     CDS             complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JNX27_002308"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7883504.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17092..17307
                     /gene="rpsU"
                     /locus_tag="JNX27_002309"
     CDS             17092..17307
                     /gene="rpsU"
                     /locus_tag="JNX27_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7883505.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17418..19163
                     /gene="dnaG"
                     /locus_tag="JNX27_002310"
     CDS             17418..19163
                     /gene="dnaG"
                     /locus_tag="JNX27_002310"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7883506.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19358..21199
                     /gene="rpoD"
                     /locus_tag="JNX27_002311"
     CDS             19358..21199
                     /gene="rpoD"
                     /locus_tag="JNX27_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7883507.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5127 a         5878 c         5462 g         4797 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagcgcg tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa
      361 ccactagcca gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc
      421 aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg
      481 cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacgtaaa aagggtctgg
      541 cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa
      601 ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg
      661 ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatcgtg
      721 gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaaccta gcggaacaag
      781 cattatcagc cgctctctct taccgcacac aggctccgct gattgactcc ttgctcaatg
      841 aaattggcgt ttcaggcggt tcactggcga cattgacttc acccttaacc tcaacaactc
      901 ccgtcgccga aaacgtagaa taaattttca gctatgaacg tccctggcac aaaaagccgg
      961 ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt
     1021 gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc
     1081 tcatcaacaa tcggggagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
     1141 gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttactc
     1201 gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg
     1261 ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc
     1321 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag
     1381 gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
     1441 gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc
     1501 ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact
     1561 ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa
     1621 tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg
     1681 cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat
     1741 cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc
     1801 gccgttggcc cagacttctt tactcccggc gatctcttct ggtttatagt cgccgccctt
     1861 caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt cttcaaacga
     1921 caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt cgagcgggtt
     1981 aacggggcgg gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat
     2041 caagcggtca cccagtttgc gggcatttgc cagatagaag acgtgtccgg cgtgcaggat
     2101 gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt ttacgcgctg ccgctacggc
     2161 cagcttcagt tcctcttcgg tcatcacgcc aaagccggta tctgcgcgcc cacgcactgc
     2221 attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc
     2281 cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc
     2341 caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct gcgcctgggt
     2401 tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt
     2461 cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc
     2521 ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg gtgtcagcag
     2581 cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacgcc
     2641 cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc
     2701 agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg
     2761 cggatcaaca ccttcgaaac cttcttcaaa atccagacgg atcagctgtt ggttgcggga
     2821 aagtacccgt aatttggtga tggtcggatg cgtcggtaca gaaacgaagt cgcatttgac
     2881 gttgacgtcg gccagagatt tactcagcgc gcgcgctgca tcgtcaatgc ccgtcaaccc
     2941 gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag ccgcgccgcc
     3001 cggacgttct tcgatggtat tcactttaac cacgggcacc ggcgcttccg gcgagatacg
     3061 actggtgggg ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc
     3121 acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg
     3181 cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccaga
     3241 tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg taatgccaga
     3301 tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca gcgccatcgc ttcctgctct
     3361 tccataatgt cgttttgcgc cagcagctcc agaatacgca cgttgtctga ccagcgcgtt
     3421 aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg
     3481 gtaattccgc cttcatcggc tttgatatca aagcgattac gatgtttatt gccgagatga
     3541 gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttctcgcggc
     3601 agtgtcataa tctcacgacg caccgcgtca aagtgcgcag tgagctgcgg atcgccgtac
     3661 actacacgcg cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt tttctgataa
     3721 tcggcaaatg ctgctgcgga tgtcaccagc attcccgccg ccccggacgg acgcagtcga
     3781 gcatccactt catacaaaat gccggaagag gtacgcgtac tgaacagatg cataatgcgt
     3841 tgcgccagac gcagataaaa ctgccgcccg tcgatttccc gctcgccgtc agtcatcgca
     3901 tccattgggc aatcatggag gaagataagg tcaagatcgg aactgtagcc taactcccag
     3961 ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc cttcacgttc gttgaggtga
     4021 tttggcttac cgtagcgggc caccatttga ccccacgcct gctgaacgac ggcatctatc
     4081 atagcttctg ccagccaggt taagtgatcg ctcactttca ttaccggtag cgtaccggcg
     4141 atatccgctg ccgcaatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc
     4201 tgttgttctt cgtcatcttc cggcacgcgc agcaaatact ggcgcagttc atcacggtag
     4261 gcatcggtcg ccgtcggctg gtaaagggta tttggatcga gcaattcatc cagcaataat
     4321 ggataacgcg ctagctggct ggcaatcatc ggcgatgcgg cacacagaga aatcaaatgt
     4381 ttaagcgccg cagggaattc actgagcaat tctaaatagg ttgtgcgggt aacaatcccc
     4441 accagcaagg cggtaatgcg cgacagcgtg acagcagcgt cttcacgcgc acagacatca
     4501 cttagcagat gtggcatcag atggtcgagc acctgacgtc ctcgcggccc gatagtacgc
     4561 ttatccagct ctttgcggaa atcagcaatc agcgttagga cctgtttgcg atcatcctcg
     4621 ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca
     4681 cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca
     4741 ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag ttgcggccag
     4801 tcagcaaagt ccatcgccca cgccagccgc gcacggttaa gctcatcaga aggaagcgtt
     4861 tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatat
     4921 gccactcgca attgttcagc atcgttttca gaaagcagat gcagcgcggc aatggcgctg
     4981 agcgttggca gtaaagagcg cgactgcagc aacggttcgc gcccaccgcg aatgagctgg
     5041 aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg
     5101 gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga
     5161 atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg
     5221 ttagcataga cgccttcgct atcgcccata atccgcgctt tgaccatcgc gtaacgctcc
     5281 cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg caaagctcag caccagcggg
     5341 ccactttcgc caaacggacg cagtcgcata tccacgcgat agacgaagcc atcctgcgtt
     5401 ggttgatcca gcactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc
     5461 agttcccggc gtccgccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata
     5521 tcagaggaga aatttagttc cccgccgccc agcttaccca tgcctaagat cagcagcggt
     5581 tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc ggcagcaggc gtcatacagc
     5641 cagtcacgcg ccgcaacaat cagcgtctcc gccagataac tgagctgctg caatatgctc
     5701 tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc gcaccataat gcgccgccgg
     5761 aatagccgca gctcgcgcat taacccggct tcgtcgctca cattactgag cgcctcctgc
     5821 aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg gttggctttc cagttccgtc
     5881 agccactctg gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt aagtactgac
     5941 ttcgcctgtg cactaagtga ttcctcggct aaaagctctg gcagccgctc aacaacggtc
     6001 tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga
     6061 ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg
     6121 ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc agcccctgcc agttctccag
     6181 ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc
     6241 acgcatcagg cgtggcaact ggtcacggta gcgatcgcct aacggctggc agaaaacgct
     6301 tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga aggagtcgct
     6361 aattttgccc tgcgctttgg catctaaaaa cggctgccat gctttgctta ccagccattc
     6421 ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag accgccgtca cggcagaaac
     6481 cgcagaagcg atggtcgctt cgcattgaat cagcaaatca cgtaagtgag tgctcgcttt
     6541 acgcggcaca atgccaccga acagcatcag tgtatgacgg actaggctaa tggctgccag
     6601 cacctgctct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat acagccattg
     6661 cgctaacacc agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac
     6721 atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc
     6781 acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc
     6841 cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc agttccagct cgcagatagg
     6901 ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga gcgatttcaa ttcggctgcc
     6961 atcgatctct actagccatt tttcgcgata aaaatcggtg ctaaacagcg gctgcacgcg
     7021 ggaagcgaga tcggcaggca attcgccgtt cggccagatt tccgtcggta actgcgccag
     7081 gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctggtgta agccgcctgt
     7141 cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa
     7201 gcccatatcg tgcctacgca gccagttatc cggcgtttcg tagtaaatat tcagcaactg
     7261 cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc
     7321 actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt
     7381 atgtcacata agtgacgatg aacggcgaac ttaatgcgat cttttttgtc agtagatagt
     7441 attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg
     7501 gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg
     7561 ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct
     7621 gatcggatta actttactcg cacttagcgc gactgccgtc tcacacgctg aagaaacgcg
     7681 ctatgtttct gacgaactga atacctgggt ccgtagcggt ccgggagatc attatcgcct
     7741 cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa
     7801 ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag
     7861 cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac
     7921 cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa
     7981 agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa
     8041 cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg atgacaaaca
     8101 gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct
     8161 gctcggtctg gtactgccgc acctgatccc aaaccgcaaa cgcaaagatc gctggatgaa
     8221 ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga
     8281 tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt tagggctatc
     8341 ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg
     8401 ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta
     8461 tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatgc
     8521 cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct
     8581 ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa
     8641 tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg tactgcgcgt
     8701 ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctgac
     8761 gctgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg
     8821 gaaagagacg gaaagcgccc tcaccacccg caatccccag gtgttcttcc aggtactgcg
     8881 cgattgcggc gcacttcgcg ttttattccc ggaaattgac gcactgtttg gcgttccggc
     8941 acctgccaag tggcatccgg aaatcgacac gggtattcat accttaatga cgctctcaat
     9001 ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca actttatgcc acgatctcgg
     9061 taaaggactg acgccgccag aactttggcc gcgtcatcat gggcatggcc cggcgggtgt
     9121 taagttagtg gaacaactat gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc
     9181 cagactggtg gctgagtttc acgatctcat ccacaccttc ccaatgctga acccaaaaac
     9241 catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact
     9301 ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag cggactaccc
     9361 gcaaggccgt tggttgcgcg aaggctggga agtggcgcag tcagtgccga caaaagcggt
     9421 cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag ctgacccgac ggcggattgc
     9481 ggcggtagcc agctggaagg aacaacgttg cccaaagcct gactgagtat aaaactaaac
     9541 gttagcacga accgtcccct cgccccttcg gggagagggt tagggtgagg ggaacagacc
     9601 agcaccggtg cgaacaatca cccccacccc ggccctctcc ctacagagcg agggagaaaa
     9661 cccggcctgc tcccgaacct tttctcaatc aaaagaacac gacatacacc gcagccgcca
     9721 caataaagcg ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg
     9781 ttttaatcgc tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga
     9841 tatcgccggt tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca
     9901 tcatcggtac cgccagcagg aacgagaact cggaagcagc gtaacggctc acgcccatca
     9961 gcatcccacc tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact
    10021 ggaaacagcc aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg
    10081 gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagcgcat
    10141 acatcacatt tatcgggtta aacaatgact taattgtgtc gtggaataat agccccagta
    10201 ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttcac
    10261 cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga
    10321 acatcactac taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg
    10381 tgtcgccctc aaatcccaac aagtgaccga caataatcat atggcccgtg ctggataccg
    10441 gcagaaattc tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca
    10501 tatcgctcat caataaaccc ctaaattatt aaaatgtacc gcttgtccga actactgcag
    10561 tatgaccagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat
    10621 tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc
    10681 tgagtttgat acgcacccac ggcgagttga agcgtgctaa cagcagctcc gccacctctt
    10741 cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc accgtttctg
    10801 caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat
    10861 cccatgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa
    10921 caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc
    10981 tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat
    11041 cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgcc tggaatgatc
    11101 ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg
    11161 ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc
    11221 ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgcct
    11281 gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc
    11341 gcctgtcttg gacacatctg gcccgttttc ttcggtttta aaggaggaaa aggcgttgct
    11401 accgcttttg gagcaatcgc accaattggc tgggatctca ccggagtaat ggcgggaacc
    11461 tggttactga ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt cagtgcactg
    11521 attgctccgt tttatgtctg gtggtttaag ccacaattca ccttcccggt ttcgatgctc
    11581 tcttgcctga tcctgctgcg tcatcatgac aacatccagc gtctgtggcg tcgtcaggag
    11641 acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg
    11701 gatctacatg acctgatagc cttcatcggg cttgcccagc cgttgctggc accatgccgc
    11761 cagaaattcc acgcagacac gaatttcatg ctgcgataga gcggctcccg gtaaacagcc
    11821 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    11881 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    11941 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12001 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12061 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12121 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12181 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12241 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    12301 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    12361 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    12421 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    12481 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    12541 tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    12601 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    12661 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    12721 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    12781 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    12841 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    12901 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    12961 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13021 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13081 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13141 cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13201 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13261 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    13321 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    13381 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    13441 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attctgcgcc cgattggcag
    13501 ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag gactcaaccg
    13561 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg catcgtcgcg
    13621 gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    13681 tatgaaaaag atcctgacaa cctcgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    13741 cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc accgccgttt
    13801 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    13861 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag
    13921 tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa aactggtggt
    13981 tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca aggcgttaca
    14041 tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag agattgaaga
    14101 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    14161 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    14221 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14281 caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa cacgatgaaa
    14341 ccttccactg aatggtggcg atatcttgca ccgctggcgg tcatcgccat tattgctcta
    14401 cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt ttttactggc
    14461 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    14521 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    14581 ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc taattcggtt
    14641 atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga
    14701 cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt tctcggctat
    14761 gcggtgatgt tctccgagct gatcctagca cctgtaacac cgtccaactc ggcgcgtggt
    14821 gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    14881 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    14941 gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat tggactgatg
    15001 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    15061 agcattttac tgcttctgct ggttccctgg ctggcttacg tgctataccc gccggtactg
    15121 aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15181 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    15241 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15301 ctgcgcatta tcagttggga cgacattgtc agtaataaag cggcatggaa cgttttcttc
    15361 tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt tattagctgg
    15421 tttggcaaac tgttagcagg cagcttaagt ggttatccgc cgacgatggt gatggtgacg
    15481 ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctcc
    15541 gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    15601 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    15661 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta ctggcgactg
    15721 ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg
    15781 cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca cccaatagtt
    15841 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc cgagatccgc
    15901 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    15961 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttcagccag
    16021 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    16081 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    16141 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgctg ttgtcacgaa tcgtgttcgc
    16201 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16261 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    16321 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    16381 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16441 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    16501 cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc
    16561 cagagagcgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    16621 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    16681 caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga cgccgccgta
    16741 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    16801 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    16861 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    16921 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    16981 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17041 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17101 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17161 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17221 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17281 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17341 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17401 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17461 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17521 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    17581 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    17641 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    17701 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    17761 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    17821 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    17881 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    17941 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    18001 gcagttacga tcgtttccgc gaacgggtga tattccccat tcgcgataaa cgcggtcggg
    18061 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    18121 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18181 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18241 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18301 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18361 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    18421 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    18481 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18541 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    18601 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    18661 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    18721 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    18781 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    18841 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    18901 agccaggtct gacctccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    18961 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    19021 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19081 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19141 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19201 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19261 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    19321 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19381 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19441 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19501 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19561 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    19621 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    19681 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    19741 ccaagttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    19801 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    19861 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    19921 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    19981 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    20041 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    20101 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20161 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20221 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20281 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20341 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg caaaaactgc agcagattga
    20401 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20461 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    20521 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    20581 taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    20641 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    20701 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    20761 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    20821 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    20881 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    20941 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21001 ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21061 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21121 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    21181 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    21241 tctctctgca caaacgccac cttt
//
LOCUS       DADLXX010000065        21254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLXX010000065 DADLXX010000000
VERSION     DADLXX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.065"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(32..1012)
                     /locus_tag="JNX27_002312"
     CDS             complement(32..1012)
                     /locus_tag="JNX27_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY7883508.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSALDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JNX27_002313"
     CDS             complement(1020..1730)
                     /locus_tag="JNX27_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY7883509.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNX27_002314"
     CDS             complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNX27_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7883510.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4143..5552
                     /gene="glnA"
                     /locus_tag="JNX27_002315"
     CDS             4143..5552
                     /gene="glnA"
                     /locus_tag="JNX27_002315"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7883511.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5725..6774
                     /gene="glnL"
                     /locus_tag="JNX27_002316"
     CDS             5725..6774
                     /gene="glnL"
                     /locus_tag="JNX27_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7883512.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6786..8195
                     /gene="glnG"
                     /locus_tag="JNX27_002317"
     CDS             6786..8195
                     /gene="glnG"
                     /locus_tag="JNX27_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7883513.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8307..8417
                     /gene="yshB"
                     /locus_tag="JNX27_002318"
     CDS             8307..8417
                     /gene="yshB"
                     /locus_tag="JNX27_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7883514.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNX27_002319"
     CDS             complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNX27_002319"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7883515.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNX27_002320"
     CDS             complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNX27_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7883516.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11299..11931
                     /locus_tag="JNX27_002321"
     CDS             11299..11931
                     /locus_tag="JNX27_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7883517.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNX27_002322"
     CDS             complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNX27_002322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7883518.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15101..15343
                     /locus_tag="JNX27_002323"
     CDS             15101..15343
                     /locus_tag="JNX27_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883519.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFIFHAVKNKSIPNN"
     gene            15463..16395
                     /locus_tag="JNX27_002324"
     CDS             15463..16395
                     /locus_tag="JNX27_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7883520.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16436..17866)
                     /locus_tag="JNX27_002325"
     CDS             complement(16436..17866)
                     /locus_tag="JNX27_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7883521.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNX27_002326"
     CDS             complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNX27_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7883522.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNX27_002327"
     CDS             complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNX27_002327"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7883523.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQESPLQLTPMY"
     gene            complement(19727..19996)
                     /locus_tag="JNX27_002328"
     CDS             complement(19727..19996)
                     /locus_tag="JNX27_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7883524.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20066..20650
                     /gene="mobA"
                     /locus_tag="JNX27_002329"
     CDS             20066..20650
                     /gene="mobA"
                     /locus_tag="JNX27_002329"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7883525.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20632..21159
                     /gene="mobB"
                     /locus_tag="JNX27_002330"
     CDS             20632..21159
                     /gene="mobB"
                     /locus_tag="JNX27_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7883526.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5181 a         5351 c         5122 g         5600 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctaaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc catcgctggc
     1921 aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga
     1981 cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc
     2041 agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc
     2101 agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg
     2161 cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact
     2221 tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg
     2281 acagctttac cctgaccgtt agagatcagt acaccgttct gacgctgacc cacttcgccc
     2341 ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg
     2401 gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt
     2461 acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc
     2521 tgcatcacag aaccctgatg ctgttcttca acgtccagcg tcacgttttc atacggctct
     2581 tgtttacgac cgtcgatttc acggaagatt actttcggac gggataccgc cagttcgaaa
     2641 ccttcacgac gcatgttttc gataagaaca gagaggtgca gttcaccacg accagaaacg
     2701 cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct
     2761 ttgttcagac gatcgaggat ctgacgagac gttacgaact taccttcttt accgcagaac
     2821 ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc
     2881 agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc
     2941 gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg
     3001 tggcccagca ctttaccgac tttcgcgtta cgggttttgc cttcgctatc gatgatagta
     3061 acctgctggt tcggcttcac tttaccacgc ttgatgcggc caatgccgat aacgccaaca
     3121 tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc
     3181 ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct
     3241 tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc
     3301 tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca
     3361 ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca
     3421 aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc
     3481 agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc
     3541 cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg
     3601 aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt
     3661 tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccaa ggtggtttta
     3721 ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct
     3781 caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac
     3841 aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa
     3901 aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat tgaagcacta
     3961 tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag catgataacg
     4021 ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac
     4081 ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag gagagttaaa
     4141 gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt
     4201 tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac tatccctgct catcaggtga
     4261 atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag
     4321 gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt
     4381 tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag
     4441 gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg cgttccactg
     4501 gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc
     4561 gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga
     4621 actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt
     4681 acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga
     4741 tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga
     4801 acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca
     4861 aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac
     4921 cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg
     4981 ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg
     5041 gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt
     5101 ataagcgtct ggtcccgggc tacgaagcac cggtaatgct ggcttactct gcgcgtaacc
     5161 gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac
     5221 gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc
     5281 ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc
     5341 tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga
     5401 acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg gatgaagcga
     5461 tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc
     5521 cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaca
     5581 acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc ttttttctcc
     5641 gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt
     5701 gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc
     5761 ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat ccattacgcc
     5821 aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacgccgtta
     5881 ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg
     5941 gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt
     6001 tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc accgatggat
     6061 aaccagcgcc gcttaagcca ggaacaacta caacatgctc aacaagtagc tgcccgtgat
     6121 ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg
     6181 cagttactca gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc
     6241 gaacaggcgg accggctgcg aaatttggtc gaccgtctgt tggggccgca gctgcccggt
     6301 acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg
     6361 gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg
     6421 cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg
     6481 ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta
     6541 cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt
     6601 ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc
     6661 gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa aattgaattt
     6721 accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga
     6781 cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc
     6841 ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac ggcgcggaag
     6901 tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg
     6961 gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca
     7021 tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt
     7081 ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta
     7141 tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc ccaacgaccg
     7201 atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg tattatcggt cggctttcgc
     7261 gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc
     7321 atgccctgca tcgccacagt ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag
     7381 ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg
     7441 gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggcacg ttatttctcg
     7501 acgaaattgg agatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg
     7561 gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg
     7621 ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc
     7681 accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc
     7741 cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt
     7801 tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc
     7861 agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc
     7921 aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac
     7981 cggacagttg ggcgacactt ttagcgcagt gggcagacag agcgctgcgt tccggtcatc
     8041 aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc
     8101 gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacactc
     8161 tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg taaagattga
     8221 ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt cagtatgatc
     8281 ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg
     8341 gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga
     8401 ttgggctgtt tagctgaagc tcgttgttta tgccggatgc ggagaaagtc gtaggcctga
     8461 taagacgcga ccagcgtcgc atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc
     8521 cttatccggc ctacaaaagc aataatttca acaggttttg ttgcccagcg catcaggcaa
     8581 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
     8641 actctttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
     8701 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
     8761 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
     8821 aagtaatcag cgaagtgcaa atcccactgt ttctcaagag gggcgtaatc cagacggaag
     8881 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
     8941 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
     9001 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
     9061 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
     9121 gccaccgcca gctcgtcatc aggacgggca aagtgatcca taccgataaa ctgatagccc
     9181 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
     9241 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
     9301 aaaacgctca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
     9361 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
     9421 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
     9481 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
     9541 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
     9601 gcatcggtat tgaaatggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
     9661 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
     9721 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
     9781 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
     9841 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
     9901 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
     9961 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    10021 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    10081 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    10141 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    10201 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    10261 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    10321 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    10381 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    10441 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc actcccaatg
    10501 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    10561 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    10621 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    10681 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    10741 tattccgtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    10801 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    10861 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    10921 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    10981 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    11041 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    11101 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    11161 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    11221 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    11281 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    11341 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    11401 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    11461 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    11521 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    11581 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    11641 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    11701 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    11761 cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    11821 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca agctgcggca gaaactggat
    11881 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    11941 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    12001 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    12061 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    12121 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    12181 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    12241 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    12301 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    12361 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    12421 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    12481 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    12541 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    12601 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    12661 gcccagctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    12721 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    12781 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    12841 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    12901 gatatctttt ccttccgcga atgcggtcag caagccttta tcacgcgaga gatgcgccat
    12961 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    13021 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    13081 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    13141 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgact tcagcttcgc
    13201 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    13261 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    13321 tttttcaaac agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    13381 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    13441 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    13501 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    13561 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    13621 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    13681 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    13741 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    13801 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    13861 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgtgag agatttgatc
    13921 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    13981 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    14041 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    14101 gatagtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    14161 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    14221 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    14281 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacatcggt
    14341 tttaatcgtc gccaactggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    14401 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    14461 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    14521 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaagtcga tgatcaattc
    14581 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    14641 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctaat
    14701 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    14761 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    14821 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    14881 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    14941 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    15001 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    15061 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    15121 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    15181 gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt catgtggata actttgtgca
    15241 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    15301 tatttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    15361 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcattt
    15421 tctgctaaaa tctgcgcctt gcgcttcttt agtgcactgt ctatggctaa ccttttgaat
    15481 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    15541 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    15601 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    15661 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    15721 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    15781 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    15841 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    15901 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtcaa
    15961 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    16021 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    16081 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    16141 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    16201 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    16261 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    16321 tggctgaact cattgtggca ggaaaaagac cggcttctga cgtctctcat atcatcacag
    16381 cgtcagaata agtaagtttt actcgcctct gaatgagcag aggcgagtga gtattttaat
    16441 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    16501 caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    16561 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    16621 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    16681 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    16741 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    16801 tccatcttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    16861 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    16921 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc actgctgagg
    16981 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    17041 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    17101 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    17161 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    17221 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    17281 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    17341 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    17401 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    17461 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactacgttc acttttgccg
    17521 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    17581 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    17641 tatcgaatgt catttgcatt tgaatacggg aattaaaaaa gcctttgtca aagtttttat
    17701 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    17761 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    17821 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    17881 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc ggcaggatgc
    17941 attatcagaa tgtatttata tttattaaat aataaaaaaa gcccgtgaat attcacgggc
    18001 tttatgtaat ttacattgaa ttattttttc tcggacagat atttcaccgt atcagcatac
    18061 tgctgaacaa aaacatccat attgctggta tccataccct gcggattcag ctgatattta
    18121 ccgttaacaa acatcgccgg aacaccacgc aattgcacat cagctgcggc tttttcctgc
    18181 tgagcgacca gagatttcac cacgaagctg ttccacgccg cgtcgtactc ttcaccttta
    18241 atacctgcat tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
    18301 tgtacgcctt caaacagcgg aacagtcact ttgtcttcca cgcccagcgc catcgccaca
    18361 gcccatgcct gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta
    18421 gtcatcttca cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct
    18481 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
    18541 gctaccggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgca
    18601 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
    18661 cgattaatac ataggtgtta attgcaaagg ggattcttgt agaacttttg cctgttctat
    18721 aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt aaccacggga aatttttcgg
    18781 gaacgcggga tcagcccaat gccgcattag ccaggcaaga taataaacca aacgcatggc
    18841 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
    18901 ttcaataata gtttccagtt gcatccgctg ctgggcttta tcgccattga gcaacatcca
    18961 caaatcctga atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
    19021 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
    19081 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
    19141 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
    19201 attcagaccg atggtcgggc gatgaataaa aagctgtttg cgccccgtct ggtgcatacg
    19261 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
    19321 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
    19381 aaaggccaca ggtgctgcga ccggaacttc atcatttacc agctgcaacg caaattgatg
    19441 ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg
    19501 tcgacgctct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
    19561 accggaatcc acccggatcc cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag
    19621 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
    19681 aaggatatca tttcacgatg attttggtgg cgccgcttac aagcttttac tctttaatta
    19741 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
    19801 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
    19861 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
    19921 acaagttaaa gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
    19981 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
    20041 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
    20101 ggcaggcggt aaagccagac gaatgggcgg cgtagataaa ggattgctgg acttaaatgg
    20161 caaaccgctg tggcaacatg tcgctgacgc gcttaagacg cagctctctc acgtcgtggt
    20221 taatgctaat cgtcatcagg aaatctatca ggcaggcggt ctgaaagtga ttgaagattc
    20281 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagctgg
    20341 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
    20401 gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca
    20461 cccgactatt gctctggtaa accgcgctat tgagccttta ttactggaat atctgcaagc
    20521 aggagaacgc cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
    20581 cgatcataaa gatgcatttg ttaacgtgaa tacgccagag gagcttgccc gatggcagga
    20641 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt
    20701 ttgaaaaaac tgatcccggc attatgcgcc agagggatcc gtccggggct gattaagcat
    20761 acgcaccatg atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct
    20821 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
    20881 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
    20941 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
    21001 gcagccggac atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt
    21061 gatgtgccgc ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct
    21121 gattttgtgg tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga
    21181 cgcagccagt gtcgcatccg acctttttct gcaccagcgc aaaaaggccc gactgggcct
    21241 tctgcttaat ttga
//
LOCUS       DADLXX010000066        21004 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLXX010000066 DADLXX010000000
VERSION     DADLXX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.066"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(703..3273)
                     /locus_tag="JNX27_002331"
                     /pseudo
     CDS             complement(703..3273)
                     /locus_tag="JNX27_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189288.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3391..4584
                     /locus_tag="JNX27_002332"
     CDS             3391..4584
                     /locus_tag="JNX27_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7883527.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKTTQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4782..6035)
                     /locus_tag="JNX27_002333"
     CDS             complement(4782..6035)
                     /locus_tag="JNX27_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7883528.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6362..7552
                     /gene="hmpA"
                     /locus_tag="JNX27_002334"
     CDS             6362..7552
                     /gene="hmpA"
                     /locus_tag="JNX27_002334"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7883529.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNX27_002335"
     CDS             complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNX27_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7883530.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNX27_002336"
     CDS             complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNX27_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7883531.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNX27_002337"
     CDS             complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNX27_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7883532.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNX27_002338"
     CDS             complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNX27_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7883533.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNX27_002339"
                     /gene_synonym="purI"
     CDS             complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNX27_002339"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7883534.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16328..17884
                     /gene="mltF"
                     /locus_tag="JNX27_002340"
     CDS             16328..17884
                     /gene="mltF"
                     /locus_tag="JNX27_002340"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY7883535.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNX27_002341"
     CDS             complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNX27_002341"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7883536.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNX27_002342"
     CDS             complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNX27_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7883537.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19286..20134
                     /locus_tag="JNX27_002343"
     CDS             19286..20134
                     /locus_tag="JNX27_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7883538.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVVQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20473..20964)
                     /locus_tag="JNX27_002344"
                     /pseudo
     CDS             complement(20473..20964)
                     /locus_tag="JNX27_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436854.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         5042 a         5674 c         5348 g         4940 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccaa ggtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtttcag cgcctgactg gcatacgcca ccgcttgcgg
     1741 gaaatctgcg cggttatatt ccagcatcgc cagcgccagg ttacagcgat aatccagcgg
     1801 gtccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg
     1861 ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgccag
     1921 tggcgctttg gcgacgtccg gcaacggcaa ctcctgcggc tgatgttcct gataactcag
     1981 tacaatattg ccgtcggcat cggagagttc aatggtcagt ctgtcaggat tgataccgtg
     2041 caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga gcaacgcgtt
     2101 gcatttgccg atttcgcgga tcgccagacg gtatccgttc aacggagaga tggcatacag
     2161 cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc gggaggcatt
     2221 ttgcaccatg cacagagaat gataaggcag gaaatactgc tcgaaacgct tctcttcgta
     2281 agcatcaagc caggtaaaat caggctggtt atcggcaaaa ataccggtca tcagttcgat
     2341 atacgggccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt cactgtgtcc
     2401 ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca gcaaaccgcc
     2461 atcttcatcg tgacaccacg cgccgacaaa atcgtactgc gatttttcgg ccatatatga
     2521 ggttggaaca ggcacatttt tatagcgaga aatgtccact ccggcggagt agtccacttt
     2581 gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac
     2641 cgcagtcaca tccggtggga aaacgctctg atgcccttca ccccctttca ctgccgggtt
     2701 ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc tggcgatttc
     2761 cagtgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac catgcatcgg
     2821 ctccgtttcg ccgacccaca ccgtctgtgc accgtcttca tgggcttcga gggtgaaatc
     2881 aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa tcccgccgga
     2941 gatccacggt cccagcagcc ccaccagcgc aggtttaatg acttcattgt gatagacaaa
     3001 atcgcgctgt ttcactttat cccatgcgcg atgcacccga ccgcccagtt ccggcaggat
     3061 catcactttg atgtagtcgt tttccagcca caccgcctgc taggatttca gggttttctg
     3121 ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc cctgataaac
     3181 gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg tcgggatctc
     3241 aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa caagacatac
     3301 tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg cggaataatc
     3361 acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa tcaacagatt
     3421 cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg cgccaataaa
     3481 tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat tttgcaggaa
     3541 ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa gccagacgcg cgggcatagt
     3601 agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgcctgaa cgtgacgccc
     3661 accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggcga atttgagtat
     3721 ttacagattg atgtaccgac gccgcaggcg ctgctgtccg aaatcgaaaa atgctggcat
     3781 cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat ccacgggcag
     3841 gttgatccgg tgaccggcgt atcgcaaacc atgccgcaag cgccgtggac aacgccagtt
     3901 gaggtgaagt atctgctgga agagaagctg ggcattcggg tgctggtcga taatgactgc
     3961 gtgatgctgg cgcaggcgga gaaatggcaa aataattcgc aggaacggga tttctgcgtg
     4021 atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat ttatcgcggc
     4081 agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg cgtcgtctgc
     4141 gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcatt aaaaaaacag
     4201 gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga aaaactgaca
     4261 accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag ctgggttgat
     4321 cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct caatattaat
     4381 cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa tactattatt
     4441 cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa aacgacgcaa
     4501 attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta tttgtatgtt
     4561 gaggcgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc cgaaggaagc
     4621 cttgatgatc ataaacgaaa aattgcctga tgcgctacgc ttatcaggcc tacacggaga
     4681 ttgcaatata ttgaatttac aagattttgt aggcctgaga aggtgttcat gccgcatcca
     4741 acatgaacaa cgagcacatt gtcagcaaat caccgtttcg cttatgcgta aaccgggtaa
     4801 cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga taacggcttc atcattgatg
     4861 ctgtccagca cgtcacacat ccagccagcc agttctttcg cttcagcttc tttaaagccg
     4921 cgacgggtaa tcgccggagt acctacacga ataccggagg tcacaaacgg gctcttcgga
     4981 tcgttcggta cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct
     5041 tctttaccgg tcaggttttt atcaaccaga tcaaccagga acaggtggtt atcagtgccg
     5101 ccggaaacca ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt agcgtttttc
     5161 gcgacctgct gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct
     5221 ttaccggcga ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc
     5281 agttttttgt acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc
     5341 gccagggttt tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag
     5401 acgccagcag caaccaggcc cgcaacgtgc gccatatcaa cgaacagata agcaccgatg
     5461 ctgtcagcga tttcacgcat tttcgcccag tcaaccacgc cggaatatgc agagaaacca
     5521 ccgataatca ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtaatcgata
     5581 tgaccggtag catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc
     5641 ggagaaccgt gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct
     5701 ggttccagca gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg
     5761 ttagcgtagt cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata
     5821 tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg
     5881 gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc
     5941 agttcgatgt gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca
     6001 tcataatcgg caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa
     6061 taaaattttt ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc
     6121 attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa cgattacctt aaggctacgc
     6181 aaggctttgg agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg
     6241 caaatttttc acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag
     6301 ggctattttt tataagatgc atttgagata catcaattaa gatgcaaaaa aggaagacca
     6361 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
     6421 ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
     6481 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
     6541 tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
     6601 aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca tcgtcggtga
     6661 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
     6721 gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
     6781 cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa
     6841 aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
     6901 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
     6961 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtatcgc
     7021 ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg ccaatgttgg
     7081 cgatgtcgtg aaactggtcg ctccagcagg tgatttcttt atggctgtcg cagatgacac
     7141 accagtgacg ttaatctcta ccggtgttgg tcaaacgcca atgctggcaa tgctcgacac
     7201 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
     7261 agttcacgcc tttgctgatg aagttaagga actggggcag tcactgccgc gctttaccgc
     7321 gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
     7381 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
     7441 tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg atctgggcgt
     7501 gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt aatttgatgt
     7561 tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt cgtcctcttc
     7621 accggtacgg atgcgaatga cccgagccac gtcaaagacg aagattttac cgtcaccgat
     7681 tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa tgtcgtccgg
     7741 tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg cgccgcggta
     7801 cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg tcatgccggt
     7861 aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt ttataatcgc
     7921 atcaatcttt ttcatgctat tccttgaaaa ggttgcctgt cttttgatct gctaaacgta
     7981 acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc gggaaagcag
     8041 tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt
     8101 ggtgatttgc agcagcttac gcaaatagtt gagttcaaaa tgattacgtg cctcaacaaa
     8161 ggttggcagc gccgtatttt caccctccag cgcctgctcc accagcgcat cactaatcac
     8221 cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacattacc
     8281 cggccagctc gcggtcatca ggcgtttcat cgcatcggta gagaacgcgc ggacaaacgg
     8341 tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa tgtcttctgt
     8401 gcgctccgcc agtgccggaa ttttcaggct gacaacgttg aggcggtaat agagatcttc
     8461 gcggaattcc ccgcgcgcca tcgcttttga cagatcacgg tgagtggcag aaataatccg
     8521 cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac
     8581 gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga gaaatagcgt
     8641 tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag taaacgcgcc
     8701 acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc cacagttaat
     8761 agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc
     8821 tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg ccaccagccg
     8881 cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct cgcgccagcg
     8941 ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat atagcgcatc
     9001 tttatcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag caacggcatc
     9061 ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca ctttctggat
     9121 ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga taactaaatc
     9181 tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg tgaccacact
     9241 gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat cgtcatcgac
     9301 caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg gatggcgcgg
     9361 gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga
     9421 gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc agttgctgct
     9481 gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac tgctggagtt
     9541 tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg tcgcgccaga
     9601 gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca atacgcgcga
     9661 ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa
     9721 aggtattttg ccagctgccg tcgtcatatt ggcgagcttg ctgacgcgac tgcgcaggca
     9781 ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt tcggttgatt
     9841 taccttgcag cgcccagatg tcactacatt cagtagaaag atagtcagcc agttgataaa
     9901 ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc tgcacgcaac
     9961 ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat aatcgttgaa
    10021 aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcagac
    10081 agtctggaca tggacagcgc ggagaaaccg gggcgtacat atcgtacgtg aagatttcga
    10141 gcactacccg gggccgaact gacaaataaa atagcctgat aggataggct gttagcatta
    10201 tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc
    10261 gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag
    10321 accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat
    10381 catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg
    10441 cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc
    10501 attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca ggcaagctgt
    10561 tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga
    10621 aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg cactgtccgc
    10681 ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc agctgctgct
    10741 atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctgatcagc
    10801 cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg
    10861 tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta accaaagaat
    10921 acgttgccca accgagcgta actcgctcgg tccacagaac gaaacgctat tgcccagaga
    10981 acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc cgataatcat
    11041 ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg attgccaacc
    11101 aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac
    11161 tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa gacgcgcggc
    11221 ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt tcaaatcctg
    11281 acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat cgagcatttc
    11341 gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt ccagcacgca
    11401 atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt cactacgccg
    11461 ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg cattcaggct
    11521 ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa atgccagcat
    11581 taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt tactctcgtc
    11641 agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta
    11701 cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat
    11761 agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta
    11821 tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct tactattatg
    11881 cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg ttgctttgag
    11941 agatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct aaaaagagac
    12001 aacttaagat aaacttatta gattacaata aaatcaatca tttaagtgtc gccagaaagc
    12061 gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc ggagttggga
    12121 gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa tgaactgatg
    12181 acttacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc atcctcgccc
    12241 cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg gtgcggcatc
    12301 ataatggtga ctcgaccact ttcagtcgta accgccgtaa taccgttcgg tgaaccgttc
    12361 gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg cagtgccacc
    12421 agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac gcgcccttca
    12481 ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacag cagagacggg
    12541 ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt gttacgcaca
    12601 aaacgtggcc acaactcgct ccccgggatc agttcacgca ggttagacat catctggcaa
    12661 ccgttacata cccccagggc cagcgtttgc ggacgatgga agaaggttgc aaactcatcg
    12721 cgtacacggt cattgaacag gatagacttc gcccaacctt caccggcacc cagcacatca
    12781 ccgtaggaga aacctccgca cgcgaccagg gcgtgaaaat cctccagtcc cgtacgtcca
    12841 gtcagcaggt cgctcatatg cacgtcgata gcatcaaagc ccccacgttg gaaagctgcc
    12901 gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt cggacgtgcg
    12961 ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag ttttacattc
    13021 aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca ctccgggttg
    13081 tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa cgtagtgcgg
    13141 ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc
    13201 tgcccaacat aatggacgca atccgcaagc ccatgctgtg ccagtacgga ctcgaccgct
    13261 tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa caacgccgcc
    13321 aggcgatcgt cacccagaga ggcgatatcc gcgtcaatgc cacaatgacc tgcaaaggcc
    13381 atttccgcca gcgttaccag caggccgcca tcagagcggt cgtgatacgc cagcagctta
    13441 cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttggac aacatcgcgt
    13501 acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt tgcgcccagc
    13561 gcgttattgc ctttgcccaa atcaatcagc agcagtgcgt tatcttcggt acaaagctgc
    13621 ggcgtgatgg tgtgacgtac atcttccacg cgggcaaatg cagaaatcac cagcgacagc
    13681 ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat cgacatagag
    13741 tctttaccca ccgggatcgt caggcccagc gccggacaaa gttcttcacc cacggcttta
    13801 acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat ccagttggcg
    13861 gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt taacgcttca
    13921 ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc acgctcgcca
    13981 atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc gcagttagcg
    14041 accggcacct gccacggccc caccatctga tcgcgcgcca ccatgccggt tacgctgcgg
    14101 tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag tacgcgtttc
    14161 accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc tttcagcgtt
    14221 tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg cagatcgatc
    14281 ggctgattat caaaatgacg atcgtgcaga gaaagatgca gttcttcggt cgcttcacca
    14341 atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg taattgatcg
    14401 gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc cagcgggctc
    14461 atgcccggtt cgtcgcttag aatatcgcgc agttcaaatt taccgccgcg tccgccgtcg
    14521 ctcaccagct ccggcatcgc gttagaaaga ccgcccgcac cgacgtcgtg gataaacaga
    14581 attgggttag catcaccaag ctgccagcaa cggtcgatca cttcctggca acgacgctcc
    14641 atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga ctgaccagac
    14701 gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc gccgagaacg
    14761 accagcttcg caccgacgtt gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg
    14821 atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc attgtggctg
    14881 ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc aaattcgttg
    14941 ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt aacaatgcgc
    15001 tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caggaattcg caggttggat
    15061 acggagaaac caaccaaacc agctttcggc ttcgcgccgc gcccggtggc accttcatcg
    15121 cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg
    15181 ttgtgagttt cgactttcat caggatgtgc gccggttcct gatggaaatt gtagcggccc
    15241 gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc ggcgttatct
    15301 ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat catcctgaac
    15361 agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat tttgtggcgg
    15421 cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg gttacgacca
    15481 agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc caggccaaga
    15541 cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac gcttgtgacc
    15601 ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa aaagaccgtt
    15661 tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt ggtcagcgta
    15721 ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg tagcccgcag
    15781 ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg acgcggggtc
    15841 accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata tttcagcagg
    15901 cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc aaaatggaca
    15961 tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg
    16021 ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg
    16081 tcttctaagc tttcggtgta cccaaggggg ggaaacgggc gtcattataa agaatctgat
    16141 gcgctgacga aaccgtttgc gtggaaataa aatcaccatc gtgaattagc aacgcgtgcc
    16201 gccaatggct gtaataagtt gccatctggc gcaggtttac gcaaaatgcc gctcatttat
    16261 gagtaaacct ttcactatta ttacgttttt tcaagctggg acgcgcacga cacagagaat
    16321 taactaattg aaaaaattaa agattaatta tctgttcatc ggcattctgg cactgctgct
    16381 cgcggtcgct ctctggccat ccattccctg gtttggtaaa gccgacaacc gtatcgccgc
    16441 cattcaagcg cggggagagt tgcgtgtgag caccattcat actcccctga cttataacga
    16501 aatcaacggg aaaccttttg gcctggatta cgaactggcg aaacagtttg ccgattacct
    16561 cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc cagctgtttg acgaccttga
    16621 taatggtaac gccgacctgc tggcggcagg actggtctat aacagtgagc gggtaaaaaa
    16681 ttatcagcct ggccctacct attattccgt gtcacaacaa ctggtttata aagtgggtca
    16741 gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa ctcaccgttg caccgggtca
    16801 tgtggtggtt aacgatctcc agaccctgaa agaaacaaaa ttcccggaat taagctggaa
    16861 ggtagacgac aaaaaaggct ctgcggaatt aatggaagat gtcatcgaag gaaaactcga
    16921 ttacaccatt gctgattctg tcgccatcag cctgtttcag cgcgttcacc cggagctcgc
    16981 cgtagcgctc gatatcaccg atgaacaacc ggtgacttgg tttagcccgt tagatggcga
    17041 taataccctt tccgccgccc tgctcgactt cttcaacgaa atgaatgaag acggtacgct
    17101 ggcacgcatt gaagagaaat acctggggca tggcgatgat tttgattacg tcgatacgcg
    17161 cacattttta cgcgccgtcg atgcggtact gccgcagtta aagcccctgt ttgagaaata
    17221 cgccgaagaa attgactggc gtttgctggc cgctattgct tatcaggaat cgcactggga
    17281 tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg atgttaacca aaaataccgc
    17341 gcaaagcctc ggcattacgg atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg
    17401 ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg ccggagaacg aacggatctg
    17461 gtttgccctc gctgcgtaca atatgggcta tgcgcatatg ctggatgccc gcgccctgac
    17521 ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta aaacagcgtc tgcctttact
    17581 tagccagaaa ccctattaca gcaagctgac ttacggctac gctcgtgggc atgaagccta
    17641 cgcttatgtc gaaaatattc gtaagtatca gattagcctg gtgggttatc tgcaagagaa
    17701 agagaagcag gctacagaag cggcgatgca actggcgcag gattatccgg cggtatcgcc
    17761 tacggagttg ggcaaagaga aatttccttt tctctcgttt ctttcccagt cgtcatcaaa
    17821 ctatttgacc cattctccct ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa
    17881 ttaatccgtc gaggattgcg cttttttctg cgctttaatt tcctggcggc gcatgcgaaa
    17941 gaagtcactg agcaacgccg cgcactcatc cgccagtatt ccttccgtaa tttccactcg
    18001 gtgattcata cccggatgat gcagcacatc cattaaagat cccgcagcgc cagttttcgc
    18061 gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg atcatcgctc cggcacacat
    18121 tacacatggt tcaagcgtga catacaacgt ggcgtcgatc agacgataat tttgcatcac
    18181 cagaccaccc tgccgcaggg ccatgatttc tgcatgtgcg gtgggatcat ggcgaccaat
    18241 cgggcggttc cagccttcgc cgattacccg attgttatgc actaataccg cgccgaccgg
    18301 cacttcccgc tcatcccagg cacgtttcgc cagcgtcagc gcgtgacgca tccagtattc
    18361 gtggctaaat tcgacttcag acaaaaagaa aactccggtt ataaaagcgc ggcgcattat
    18421 acacggacgc tatgctttac tctattccag ttgctggagt tcaccgcgcg gggttactcg
    18481 ccaacgatgc tgacagaaat aaagcagcgg attgtcctga ttactgtcgc tatagccact
    18541 gtacagccgc agcggcgtgc cgattttgcg ctccagttgt gcgacctttt catgtcccag
    18601 acaacgcatc gtcaataccc aaccaccata gccacgctga atttggctgg cgataagatt
    18661 aacccgcggc agccagggcg tatcgaaata aaccgcttca accagcggct gcggagagcc
    18721 ggtaatcaac cagatatcag catcggaact taacaggtag gtggttaatc gctcctgaac
    18781 cagcggaaag gcggtaacat tgtcgcgaaa ccagcgcacg aaatcggcct gcaacgtctg
    18841 taaacgtgct tcgctgtgac caaaagtgca cccccacaga agcagactca tcggccagcg
    18901 tgccgcacga ccttttatca ataacgcaat ggctataatc ggtaacaacg gcaggacaag
    18961 taacgcattc agcggttggc gacgcagtaa atagcgcaga aaactgccga acatatcctg
    19021 ctgatgcaat gttccatcta agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc
    19081 atactcctct ggactgaaac atcctgatta attttcgcta ctacatagcc taacagatag
    19141 atcatcactt ttccggcaac aatcgaccgc atcatggcta actggaattt ttaattcatg
    19201 gcaattagcg gcaatggaat ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg
    19261 ccactatttc atcgcgagga aagatatgaa cggcttactt cgtatccgtc agcgttacca
    19321 ggggcttgcc cagagtgata aaaaactggc ggattatctg ctgctacaac ctgatacggc
    19381 gcgccattta agctctcagc aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt
    19441 gaagttcgcg caaaaactcg gctataaagg ttttccggcg cttaagttgg cgttgagtga
    19501 agcgctggca agccagccgg aatcgccctc cgtgcccatt cataaccaaa tccgcggtga
    19561 tgatccgtta cggctggtcg gcgaaaaact gattaaagaa aatactgccg ctatgtacgc
    19621 aacgctaaac gttaatagtg aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc
    19681 gcggcggata attctgaccg gtattggcgc ttcgggtctg gtggtgcaaa actttgcctg
    19741 gaagctgatg aagattggct tcaatgctgc cgcactgcgc gatatgcatg cgctactcgc
    19801 aacagtacag gcgtcgtccc ctgacgatct gttattagcc atttcctaca ccggtgtacg
    19861 acgcgagtta aacctggcgg cagatgagat gctgcgagtg ggcggaaaag tgctggcgat
    19921 taccggcttt actccgaatg ccctgcaaca gcgtgcttct cattgcctgt ataccattgc
    19981 cgaagaacaa gcgacaaaca gtgcttcaat ctctgcttgt cacgctcagg gaatgttaac
    20041 ggatttgctg ttcattgcgc tgattcagca ggatctggaa ctggcaccag aacgtattcg
    20101 tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc
    20161 ccggtttgtg ttgttttgag agtttcctta tggcgctgtt aatcactaaa aaatgcatca
    20221 attgtgatat gtgtgaaatc aaataaaaac acatttattt tcatagtgat acagcaatgt
    20281 gttattgaat gtgttgttgg atttatggtg caaaaaaatt ttgcataagt tcagcaacac
    20341 aaagaacacc actttcttta acgtcctccg cgccctaaag gacggggctt tacggcgcaa
    20401 aaggcctgtg acatacaaca ttaacttttc agtcaaccgt agcacgtcct tgtatacgcc
    20461 gtgtctgcgg ttttatataa ccctggctgg aaacctctta tacaaagtcg attcgccaac
    20521 atcatagatg atcgccacct tctggcgaga aactcctgat gcaattagtc gcccggcctg
    20581 cgcccattat tctggtgaaa gtttgggacg gcgaccgcca attcgtccct gtgcgcgagc
    20641 agcttccagt cctgcttttg ttcgttcaac aatcagttca cgttccattt cagccagggc
    20701 acccatcaca tggaagaaaa agcgtcccat tggtgtgctg gtatcaattg aatcagtcag
    20761 actacgaaag ttgatgcctc gttcgcgcaa ctcctccacc agcacgacaa gatgccgcat
    20821 actgcgcccc agccgatcca gcttccagac aaccagtgtg tcgcctgccg ataatgtcct
    20881 gagcagtttt ttcagccccg gtctttcgga ctttgtacca cttatcttgt cttcaaaaat
    20941 cagctcgcat cctgcacagt tcaacgcatt acgttgtaga tcggtattct ggtcatttgt
    21001 tgat
//
LOCUS       DADLXX010000067        20686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLXX010000067 DADLXX010000000
VERSION     DADLXX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.067"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNX27_002345"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNX27_002345"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7883539.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JNX27_002346"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JNX27_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7883540.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JNX27_002347"
     CDS             complement(2649..3200)
                     /locus_tag="JNX27_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7883541.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JNX27_002348"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JNX27_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7883542.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JNX27_002349"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JNX27_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY7883543.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNX27_002350"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNX27_002350"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7883544.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNX27_002351"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNX27_002351"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7883545.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNX27_002352"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNX27_002352"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7883546.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLTKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNX27_002353"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNX27_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNX27_002354"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNX27_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7883547.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JNX27_002355"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JNX27_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7883548.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JNX27_002356"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JNX27_002356"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7883549.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNX27_002357"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNX27_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7883550.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNX27_002358"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNX27_002358"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7883551.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNX27_002359"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNX27_002359"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7883552.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAACLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSQTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNX27_002360"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNX27_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7883553.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JNX27_002361"
     CDS             complement(18174..18569)
                     /locus_tag="JNX27_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7883554.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTEVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNX27_002362"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNX27_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7883555.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="JNX27_002363"
     CDS             19485..20078
                     /locus_tag="JNX27_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7883556.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /locus_tag="JNX27_002364"
     CDS             20223..20675
                     /locus_tag="JNX27_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7883557.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4917 a         5208 c         5488 g         5073 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc ggatggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcactgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaaa catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agtcagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt caggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggtggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtctgg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcacgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 acctccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaagcattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgc
//
LOCUS       DADLXX010000068        20668 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLXX010000068 DADLXX010000000
VERSION     DADLXX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.068"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNX27_002365"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNX27_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7883558.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNX27_002366"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNX27_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7883559.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNX27_002367"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNX27_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7883560.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIHEQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNX27_002368"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNX27_002368"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7883561.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JNX27_002369"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JNX27_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7883562.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JNX27_002370"
     CDS             complement(8217..9110)
                     /locus_tag="JNX27_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7883563.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNX27_002371"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNX27_002371"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7883564.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JNX27_002372"
     CDS             complement(10007..10189)
                     /locus_tag="JNX27_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7883565.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JNX27_002373"
     CDS             complement(10241..11473)
                     /locus_tag="JNX27_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7883566.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNX27_002374"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNX27_002374"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7883567.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVVMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNX27_002375"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNX27_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7883568.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="JNX27_002376"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="JNX27_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNX27_002377"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNX27_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7883569.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNX27_002378"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNX27_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7883570.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNX27_002379"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNX27_002379"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7883571.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /locus_tag="JNX27_002380"
     CDS             complement(19788..20552)
                     /locus_tag="JNX27_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7883572.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAGMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4968 a         5713 c         5017 g         4970 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcaaacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgt ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc atattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaaacct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcaccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc accataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca ttccagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaacg ccgacaat
//
LOCUS       DADLXX010000069        20661 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLXX010000069 DADLXX010000000
VERSION     DADLXX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.069"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            8..1159
                     /gene="tyrP"
                     /locus_tag="JNX27_002381"
     CDS             8..1159
                     /gene="tyrP"
                     /locus_tag="JNX27_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7883573.1"
                     /translation="MLAMPLAAAGVGFSVTLILLIGLWALMCYTALLLLEVYQHVPAD
                     TGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGAGELLASSISDWTGISMSATA
                     GVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVMLVLLLPHIHKVNLLTLPLQQ
                     GLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVFIIGSAIPLVAYIFWQVATLG
                     SIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVHLFADLALATSFLGVALGLFD
                     YLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFVMALGYAGVALAVLALIIPSL
                     LTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQFLIAAGLLPEVG"
     gene            complement(1221..1886)
                     /gene="yecA"
                     /locus_tag="JNX27_002382"
     CDS             complement(1221..1886)
                     /gene="yecA"
                     /locus_tag="JNX27_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7883574.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(2082..2168)
                     /locus_tag="JNX27_002383"
     tRNA            complement(2082..2168)
                     /locus_tag="JNX27_002383"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2132..2134),aa:Leu,seq:taa)
     gene            complement(2181..2254)
                     /locus_tag="JNX27_002384"
     tRNA            complement(2181..2254)
                     /locus_tag="JNX27_002384"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2220..2222),aa:Cys,seq:gca)
     gene            complement(2536..3084)
                     /gene="pgsA"
                     /locus_tag="JNX27_002385"
     CDS             complement(2536..3084)
                     /gene="pgsA"
                     /locus_tag="JNX27_002385"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7883575.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(3141..4973)
                     /gene="uvrC"
                     /locus_tag="JNX27_002386"
     CDS             complement(3141..4973)
                     /gene="uvrC"
                     /locus_tag="JNX27_002386"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7883576.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(4970..5626)
                     /gene="uvrY"
                     /locus_tag="JNX27_002387"
     CDS             complement(4970..5626)
                     /gene="uvrY"
                     /locus_tag="JNX27_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7883577.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            5922..6098
                     /locus_tag="JNX27_002388"
     CDS             5922..6098
                     /locus_tag="JNX27_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883578.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            6085..6309
                     /locus_tag="JNX27_002389"
     CDS             6085..6309
                     /locus_tag="JNX27_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7883579.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(6342..6962)
                     /gene="sdiA"
                     /locus_tag="JNX27_002390"
                     /pseudo
     CDS             complement(6342..6962)
                     /gene="sdiA"
                     /locus_tag="JNX27_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(7192..7944)
                     /gene="yecC"
                     /locus_tag="JNX27_002391"
     CDS             complement(7192..7944)
                     /gene="yecC"
                     /locus_tag="JNX27_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7883580.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(7941..8609)
                     /gene="tcyL"
                     /locus_tag="JNX27_002392"
     CDS             complement(7941..8609)
                     /gene="tcyL"
                     /locus_tag="JNX27_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY7883581.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(8624..9610)
                     /gene="dcyD"
                     /locus_tag="JNX27_002393"
     CDS             complement(8624..9610)
                     /gene="dcyD"
                     /locus_tag="JNX27_002393"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7883582.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(9715..10515)
                     /gene="tcyJ"
                     /locus_tag="JNX27_002394"
     CDS             complement(9715..10515)
                     /gene="tcyJ"
                     /locus_tag="JNX27_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7883583.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(10603..11154)
                     /gene="fliZ"
                     /locus_tag="JNX27_002395"
     CDS             complement(10603..11154)
                     /gene="fliZ"
                     /locus_tag="JNX27_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7883584.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(11200..11919)
                     /locus_tag="JNX27_002396"
     CDS             complement(11200..11919)
                     /locus_tag="JNX27_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY7883585.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDAPQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(12084..13736)
                     /locus_tag="JNX27_002397"
     CDS             complement(12084..13736)
                     /locus_tag="JNX27_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY7883586.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            14002..15408
                     /gene="fliD"
                     /locus_tag="JNX27_002398"
     CDS             14002..15408
                     /gene="fliD"
                     /locus_tag="JNX27_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7883587.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            15433..15843
                     /gene="fliS"
                     /locus_tag="JNX27_002399"
     CDS             15433..15843
                     /gene="fliS"
                     /locus_tag="JNX27_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7883588.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            15843..16208
                     /gene="fliT"
                     /locus_tag="JNX27_002400"
     CDS             15843..16208
                     /gene="fliT"
                     /locus_tag="JNX27_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY7883589.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            16286..17773
                     /gene="amyA"
                     /locus_tag="JNX27_002401"
     CDS             16286..17773
                     /gene="amyA"
                     /locus_tag="JNX27_002401"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7883590.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(17807..18220)
                     /locus_tag="JNX27_002402"
     CDS             complement(17807..18220)
                     /locus_tag="JNX27_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7883591.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            18407..19612
                     /gene="yedE"
                     /locus_tag="JNX27_002403"
     CDS             18407..19612
                     /gene="yedE"
                     /locus_tag="JNX27_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7883592.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            19609..19842
                     /gene="yedF"
                     /locus_tag="JNX27_002404"
     CDS             19609..19842
                     /gene="yedF"
                     /locus_tag="JNX27_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7883593.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            19949..20619
                     /locus_tag="JNX27_002405"
                     /pseudo
     CDS             19949..20619
                     /locus_tag="JNX27_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         5025 a         5352 c         5051 g         5233 t
ORIGIN      
        1 cgcaggcatg ctggcaatgc cgctggctgc ggccggtgtt ggttttagcg ttacgttaat
       61 cttgttgatt gggctttggg cgttgatgtg ctacacggcg ctattactgc tggaggtgta
      121 ccagcatgtt ccggcagata ccggtctggg cacgctggca aaacgctatc tgggacgcta
      181 cggtcaatgg ctgacgggct tcagtatgat gttcttaatg tatgctctga ctgcagcata
      241 catcagcggt gccggtgaat tgttggcctc cagcatcagc gactggacag gtatttcaat
      301 gtcggcaacc gctggcgtgc tgttgttcac ttttgttgcc ggtggcgtgg tttgtgttgg
      361 aacatcgctg gtcgatttat ttaaccgttt tctgttcagc gccaaaatta tttttctggt
      421 ggtgatgctg gtattgctgc taccgcatat tcacaaagtg aatcttttaa ccctgccgtt
      481 gcaacagggg ctggcactgt ctgcaatccc ggtgattttt acgtcgtttg gttttcacgg
      541 tagcgtgccg agtattgtca gctatatgga tggcaacatt cgtaagctac gctgggtgtt
      601 tataatcggt agtgccatcc ccctggtggc atatattttc tggcaggtgg cgacgcttgg
      661 cagcattgat tcaacaacct ttatgggatt gctggctaat catgctggat taaacgggct
      721 gttacaggcg ttacgcgaaa tggtgccctc tccgcatgtt gagctggcgg tgcatttatt
      781 tgctgattta gccctcgcca cgtcatttct cggcgttgcg ttaggcttat ttgattatct
      841 ggctgatttg tttcagcgtt caaataccgt tggtggacga ttgcaaactg gtgcaattac
      901 ctttctgccg ccgttggcgt ttgcactgtt ttatccacga ggatttgtga tggcgctggg
      961 ttacgccggt gtggcgctgg cggtactggc attgattatc ccttcactat tgacctggca
     1021 aagcagaaag cacaatcctc aggcgggtta ccgggtcaaa ggtggtcgtc cggcgctggt
     1081 ggtggtgttt ctctgtggta ttgctgtgat tggcgtgcaa tttttgattg cggcagggtt
     1141 gttaccagaa gtggggtgat cagatagcct caaattcctt attgggtgcc agaattaacg
     1201 ctgacaccca atgtgacctc ttaatgcagg cagcactgct taaatttctt accactgccg
     1261 cacggacaag gatcgttacg ccccggtttc tcttctgctt tgatcggttg ctgaacagct
     1321 ttttcctgcg gatgcgccat ccagtatgca tgtaaatcaa gcgccgccag tcgaatggca
     1381 tctacgctct cttcaaacgc ttctggcgac attttctcta cccgctcgaa gttttcctca
     1441 gtaccgtgca gcgcaatcgc ctccagcgct ggttttaaag aatcgggcaa cgttgaccag
     1501 tcagaaagtg ccacgccccg catatagcca aagcaccact cctcaacaat cgtcagctcg
     1561 ctgccatcaa cttctcgcaa gccgaataac ggctcaaact gctccgggaa ttcgttcaga
     1621 cgctctgcgg tatcggccat atgttgaaaa gccaggttca taaagcgcgt catctctttc
     1681 tctgacgccc aacgcggcac atagtcagcc ccaccccaca cggcaaccag ccactgttct
     1741 ggttcaatct cttgcggaga actcaacacc gccgtcaata aaccgtccag ctccgccaca
     1801 tcaaggatgg cgtggtcagt gttgtatttg gtcagaatat cgtccagcca ttccaactca
     1861 ctttcgttta acggtcccgt tttcatacgc ttttccttgt ggatctcaac tcgccagcac
     1921 ctatcttaca tgccggtccg tatcagagat actttttgag tggctttgcc ggtgattaaa
     1981 aattaaggag ggtgtaacga caagttgcag gcacaaaaaa accacccgaa ggtggtttca
     2041 cgacactgct tattactttg attttattct tatctttccc atggtacccg gagcgggact
     2101 tgaacccgca cagcgcgaac gccgagggat tttaaatccc ttgtgtctac cgattccacc
     2161 atccgggctc gggaagaaag tggaggcgcg ttccggagtc gaaccggact agacggattt
     2221 gcaatccgct acataaccgc tttgttaacg cgccaaattc ttcaggcctt tcagccagac
     2281 atccgcttga cgccgatgtc ttttaaactg gagcgggaaa cgagactcga actcgagacc
     2341 ccgaccttgg caaggtcatg ctctaccaac tgagctattc ccgtattcat caagcaatca
     2401 gttaatcact tgattttatt atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt
     2461 ctacttacct ggcgcgatga gtcaacgata tttttcacca cttttgatcg tttgctgaaa
     2521 attacgccga aacgatcact gatcaagcaa atctgcacgc gcagcgctca aatattgcaa
     2581 cattgaccac agagtcagta ccgcagccac aaagaaaagt gcaataccgg cgtactcaac
     2641 ccaaatgttc ggacgccaca gcagccaggc caacgccacc atctgggcag tggttttcac
     2701 tttcccaatc caggagacgg ccacgctact gcgtttaccc aactccgcca tccattcgcg
     2761 tagcgcagaa ataataattt cacgggcgat catcgttgcc gccggtaatg tcacccacca
     2821 gctgtgataa tgctcggtta ccagcaccat ggcgatagcc acgagaactt tatctgccac
     2881 agggtcaagg aaagcaccaa accgggtact ctggttccag cggcgtgcca gaaaaccatc
     2941 gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaggt
     3001 gacaggcaga taaaagacca atacaaagaa tgggataagg atgacacgga acagtgtaag
     3061 caacgtaggg atattaaatt gcataatgac gggtaactat ctgttgtcag taagattacc
     3121 cctatgttgc tacagagaca tcaatgtttc aacgaccaga agatcttttc tgccagacct
     3181 tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa
     3241 ccgcccatat atttcaacaa catttgccga cgttttggcc cgacgccttc aatggtttcc
     3301 agggaactgg tatttttgac cttcgcccgt tttttacggt gcccgccaat cgcgtgatca
     3361 tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg gtgaatctgg cggcaaacta
     3421 aatccctcac cttccggctc aaagaacagc gtttccagtc cagccttacg atctgctcct
     3481 ttggcaacgc caagtagcag cggatgattt ttatcccatg agacatccag ttcggcgaag
     3541 acatttttcg cctgcgcaag ctggcctttg ccgccgtcga taaggatcac atccgggatc
     3601 ttactgtcgt caatggcttt accataacgc cgacgcagca cctgattcat cgccgcataa
     3661 tcatcgcccg gcgtgatgcc tgtaatgtta tagcgccgat actccgcacg cagcgggccg
     3721 ttagcatcaa acaccacaca ggaagcgacg gtttgttcgc ccatggtatg gctgatgtca
     3781 aagcactcca tccgcttcac ttccggcaat ttcaacacgc tggcaagcgc ggtcagtcgc
     3841 tggtgaacgg tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc
     3901 gcgagtttca gataacgcgc cctatcgccg cgaggtttgg tttgaacatt aatcttgcgt
     3961 cccgccagtt ctgaaaggga atcggcgagc agcgttttat cgctaagatt aaaatcgagc
     4021 aggatctcac ccggtaaggt gcgcatctgg ctgccttgta aatagaactg acctacgaag
     4081 gtttctacca cctcgctcag ttccgtaccg ccaggcactt tcgggaaata gctgcggctg
     4141 ccgagcactt tgccctgacg aatgaacaat acgtggacac aagccatgcc cgcatcgaac
     4201 gccacaccaa taacgtcaag gtcgtcgccg gtattggaaa caaactgttt ttcggtgacg
     4261 cgtcgcaccg cctgaatttg gtcgcgaata catgccgctt cttcaaactc cagattctgg
     4321 ctggcagttt ccatacggct aatgagttgc gtaagcacct gatcatcttt gccagacaaa
     4381 aacaggcgca catactcgac ctgctgagcg tattcttctt cactcaccag tccttcaacg
     4441 cacggtccca gacagcgccc tatctggtat tgcagacacg gacgcgagcg attgcgataa
     4501 acactatttt cgcactggcg aatggggaaa atcttttgca gtagcgccag tgtttcacgt
     4561 acggcatagc cattcgggaa cgggccgaaa tattcacctt tggcatgctt tgcaccacga
     4621 tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata agatttatca
     4681 tcgcgtagca aaacgttgta acgcggctga tagagtttga tgtagttgtg ttccagcaac
     4741 agcgcttcgg tttctgtatg cgtaaccgtt acatcaattt gctggatctg ggcgaccagc
     4801 gcttcggttt tgcgcgaagc gaggttgctg cggaaatagc tggaaagccg ttttttcagg
     4861 tctttcgctt tgccgacata gataaccgta ccaccagcat cgtacatgcg ataaacgcct
     4921 ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa actgatcact cactgacttg
     4981 ataatgtctc cgcattacac agaccatggc gaattgccag gtgagtcagc tcaacatcgc
     5041 catgaatgtt tagtttactg aacatacgat agcggtagct gttcaccgtt ttcggactga
     5101 gattgagctg ttctgagatc tcattgacct tctggccctt agtgatcatc agcataatct
     5161 gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt tttttctggt tcgatctggc
     5221 ttaacgccat ttgttgagcg atatcagaag caatgtaacg ctgccctgaa tagacagaac
     5281 gaatcgcact cacgacttcc tgcggagccg cgcctttgct gaggtagcca gcagcacctg
     5341 cctgcatgac tttcgctggt aaagggtttt ctgtatggac cgtaagcatg atgattttaa
     5401 catcagctgt ggaacgcgcg attttacgcg tcgcctcaag accgccaatg cccggcatac
     5461 tcatgtccat tagcaccacg tcaacggcat ttgtccggca ccacttaacg gcgtcttcac
     5521 cgcacgatgc ctctccgacg acttttatac cctttatatc ttccagaatg cgtcgtatcc
     5581 ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga atatctccag
     5641 aaatagggat aacgctactg atagttagtc attcgtatat tagcggtttt tattgcaact
     5701 ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa ttcgattgtc
     5761 cacagttatg gtcacgcccg tcaccaacgg cttaccggat cgtttcacag taagccctac
     5821 gatgaaatgt gacaaaaatt gactttattc agcaaaaatg aaaatcagcc cgctgaatat
     5881 gtaacattaa ttaacccgaa caatacgggc aaaaaacatt catgataaaa atatttattg
     5941 gtcattatat taacgtattt tatagcactg ccgatatcac gctcaaaaaa caaccactgc
     6001 tatttttagc aaagcttatg gtatactccg ccgccttaac atttttcacc gcaaattttc
     6061 attgcaacat gacgaggaaa ataaatgagt acgcctgatt tttctactgc cgagaataat
     6121 caagaactgg caaatgaagt ctcctgcctg aaagcgatgc tgacgctgat gctgcaggcg
     6181 atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg aaaaacagct tgcgctgatc
     6241 gaagacgaaa cccaggctgc agtattttcc aaaacggtta agcaaattaa acaagcctac
     6301 cgtcagtaat ataaaaccgg ctgagagcgt gcctttcagc cggttattga caaaatcatc
     6361 gctatctctg ctgatgtctt cccttccgcc gtccatttca gaatttcttt ttcgcgcttg
     6421 ctgaaattca tctctggcgt catcactatt tcatcattta aacgcatcag agccatcaga
     6481 ctttcgcgca ccagtaactg catttttaat tgcagttcat cactaagaat gggtatttcg
     6541 cgcgtgctgc aacgggaaaa ggacaaaaag cccagcgccc gatttggcag cattaaatac
     6601 tgagtgacac cgcggcgtaa accatgtgcg tgcgcggctt cccataatgg ctgtgcttcg
     6661 ctgaataagt catcattcca cattaaatgg tcctgactaa agttttcagg gttcagcaca
     6721 ggatcaattg cgagaaaatt ttttgcctga taataactaa cccacgactc agggtaattg
     6781 gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt ggcgaacaca taacgaatag
     6841 taatcgtact ccagctgctg agcctgaagc tctatttcat ggtagacctc ttctgcggcc
     6901 tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc tgaaaaaatc cgtatcctgc
     6961 atagtaaacc gcaacgcccc tgagagtgag tatcatttat aatgataatt caaactatca
     7021 cataaataag atttatatat aatttatatt attcaggcaa tgaattactt ttgcaagcca
     7081 tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt tatcacgctt
     7141 gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt attattgcag
     7201 cagaagcttc tcgaggaact ggcgggtgcg aggctgctgg ggatcggcaa ataacgcttt
     7261 tgctgccccc tgctcgacta tccgcccctg gtccataaag atcgcccggt ccgcaacatc
     7321 ccgggcaaag ctcatttcgt gtgtcacaat caccatcgtg cgcttttcct gcgccagctg
     7381 gcggatggtg ttcaggactt cgcccaccag ctctggatcc agcgccgacg ttggctcgtc
     7441 aaacaaaatc acctcaggac gcattgccag tgcacgcgca atcgcaacac gctgctgttg
     7501 acctccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa ctttcgccag
     7561 cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga tcaccggccc
     7621 ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct ggaagacaaa
     7681 cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc tgacttaatg aacgtgcagt
     7741 atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca gcagatttat
     7801 gctacgtagc aatgtggttt tgccggaacc actcggaccg ataattgcca ccacttcgcc
     7861 aggctttacc tcgaggtcga taccgtgcag caccgtctga ccgtggaatt ttttcaccag
     7921 gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag ttgattctca
     7981 aaatggttct gcaacgtcga taacacagtc gccatgatcc agtagatgag cgaagccgcc
     8041 aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg acggaacagc
     8101 tccggcacct ggattgtcgc ggccagcgac gtatctttta ccagactgat aaagctgttc
     8161 gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg catggtctgc
     8221 cacggcgtca taccaatact ggcagcggct tcccactgac ctttatcaat agaagaaatg
     8281 gcggcgcgca gcgtttcggc ggcataggcg gcagtgttca gcgacagacc aatcattgct
     8341 gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat cataaacagt
     8401 tgtgcgatga gcggcgtacc acgaaaaata gagatataaa aacgcgccag ccagcgcacg
     8461 ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagtagtaa gccaaaaaac
     8521 atgccgccga tactgagttg cagggtatac ccagcccctt tcaacaggaa cggcaaggaa
     8581 tcaataacca gttgtatact ttcttgcatg agcgttcccg gagttaaacg tggggatgat
     8641 aggcgaacag cgcaggcgcg ccgccggtat gaataaacag aatcggccct tcatctttga
     8701 agcgtttctg actgataccg tcaatcagcc ccgccatcgc ttttccggta tacacaggat
     8761 caagcagaat accttccaga cgcgccagca atttcactgc ttccatgcct tcgtcgttcg
     8821 gcacgccgta gccaggtgca aaatagtcat cccagagtaa aatttccgct gatgcggtca
     8881 gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaat tgatcggcaa
     8941 cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt tccagcccaa
     9001 cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac taccaccgac gaaatattaa
     9061 ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct agcgcgccaa
     9121 gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct tcgactcgcg
     9181 tcgccagctc ttccagttgg gcattgggat cggtcagtgc gtcgcacatt tcaatctggg
     9241 tattgaacag atccagcaac aaacgattgc cgttggttaa atagttttct gcggttgtgc
     9301 caataggatt ttccagcagc gccacgcagt gcagaccgag tttcgccgcg actgcggcag
     9361 tctggcgcac atggttagac tggatcgcgc cggcagtaat cagcgtatcg gcaccttcgc
     9421 gcagggcatc tgccgcgaga aattccagct tacgtaattt attgccgccc attgccatgg
     9481 gggtgacctc atcccgtttg atgaaaattt cccgtccaag ataatcagaa aagcgcggca
     9541 gatattcgag cggcgttggc gcgccgataa actccaaccg tggaaaacgg gttaaattat
     9601 gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt tcttattatg cacgctgaaa
     9661 acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt tgtcattatg ctgattattt
     9721 ggtcacatca gcaccaaacc atttttcgga aagagcttgc agagtgccgt ctttttgcat
     9781 ttccgcaatt gcatcattca ccgctttcag caggtcttca tttcctttac gcagcgccac
     9841 gccagactcc tgacgggaga atgcttcacc ggttactgcc agcgtatcgt tggttttctt
     9901 caccagatcc agcgccgcca gacgatcaac gaggatcgca tcgatacgcc ctacgcgcag
     9961 atcctgatat ttggtcgggt catcatcata ggtacgcaca tcgacaccct gaacattctg
    10021 ccgcagccac tcttcatagt tggtgcccag accgacaccc acttttttgc ctttcagatc
    10081 agcagccgtt ttaatggtgc cttcgttacc ttttttcacc agcgcctgaa taccggaaat
    10141 ggtgtaaggg gtagagaaat cgtatttttt cttgcgctcg tcagaaatgg tgacctgatt
    10201 aatcaccaca tcaatgcgtt tagagtccag cgacgccagc ataccgtccc atttggtcgg
    10261 ttttagtgac gcctcaacgc caagatgttt tgccagctgt tgggcaaatt ccacttcaaa
    10321 accggttaat ttgccgtcat ccccctgaaa actgaacggc ggataagtgc cttccagccc
    10381 taccagcagc gtgccgcgct ctttgacttt attaagcaga ccttcatctg caaaactttt
    10441 aacgctcatg cccgcaacca gcgccacgcc catcacaccc atcaatgcct gacgtcccag
    10501 atgtgctaat ttcatattca ccccgaatgt tgttatgtct gtttgcagtg tagagccatt
    10561 gtttgtaaac acaaaaacaa ctccgctaca tcttattctt atttaatata tatcagaagc
    10621 aggcaggctg gaggatttct gcacaagtcc ggtacaggtt tggcgctgat agtgttgata
    10681 cttccgtaat gcgatgcggt aattgttggt gctggtggtg ggcagccacg gggcgaggat
    10741 ttcatcaagg aaaccgtcct gcagctgatc gagggaaata ttttgctcat gtagatgatt
    10801 acccaaacgg cgcagacgaa cgacatattc acgcaccgta cctgggctca tttcagtttg
    10861 ctcaaaaaga aatttcttaa agccgataat atcgaaaaaa tcactctgcg ttttgcaatg
    10921 taaatcaccg caaaatcggc acaatgccgc ccatgattgt tgttccgttt gccagccttt
    10981 ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta ttcactattt tgccgtcgcg
    11041 aactaaggta atgcgatcga gtaatttacg gcaatgcgcg caatgggtct ggctgtgttt
    11101 aaagtcttta agatagcggc ttaatggccg tcttttcagg tgctgcacca tcattaagaa
    11161 ctcctggtag tcaaagttaa agtgcggcat ttactgacgt tataacttac ccagtttagt
    11221 gcgtaaccgt ttaatggcct ggctgtgtaa ctgactgacc cgcgattccc cgacctccag
    11281 caccgcgcca atctctttga gattcagctc ttcctggtaa tagagggtta ataccagttt
    11341 ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc tggcgcagat tactgtccag
    11401 cagttgttgt agcgggtttt ctcgctgatg atcatcagta accagttcga tgctatcgcc
    11461 gtgctcttca cgccactcat cgtaggagaa gagctggctg ttattggtgt cgagcagcat
    11521 ttgacgataa tcggcaatat cgatccctaa acgttccgct acctcagttt ccgtggcgtt
    11581 gcggccaagt tcctgctcca gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg
    11641 cacgctgcgc ggcacccagt cacggctgcg aagttcatcc agcatagcgc cacggatacg
    11701 ctgcactgcg taagttgtaa atgccgttcc ttgtggggcg tcatagcgtt cgacagcatt
    11761 aagtaaccca atgccgcccg cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg
    11821 aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc acagcgagtg
    11881 tttatccatt acaccttcag cggtatagag tgaattcacg ataaacagcc ctgcgttata
    11941 tgagttatcg gcatgattat ccgtttctgc agggttttta atcggacgat tagtgggtga
    12001 aatgaggggt tatttggggg ttacgcaaaa agccgaatgt aaaaaacccc gtcggtggcg
    12061 gggtttgaga ggcattcaga cgattaaccc tgcagcagag acagaacctg ctgcggtacc
    12121 tggttggctt ttgccagcac ggagttaccg gcctgcagga taatctgcgc tttcgacatg
    12181 ttggacactt cggtcgcata gtcggcgtcc tgaatacggg actgcgcttc agacaggttg
    12241 gtagtggtgt tgttcaggtt ggtgactgcg gaatccaaac ggttttgcac cgcacccagg
    12301 gaggagcgga atttgtcgat ggagctgatg gcttcatcca gagctttcag gggatcggct
    12361 gtcgtagctg cttttgtttc agcatctgtc gtcaatttac catcagcatc tttataaact
    12421 gccttaccgc tgccgttagt cactgaacca tcatcctgta ggtaaaaatc tacagtatca
    12481 gatgtggtca acgcctcgtt gttatcaaca taaaccgaat caacaccttt tgttacagcg
    12541 ccagttgtgc cgttgacgct ataacctgca gtagtgaaat caacattgtt tcccgaggtt
    12601 gatacagctt tatcgaaagc tgcctgatca acttttgcac ctgttactga ataagtaata
    12661 gtgtccggcg tagtactatt accggtaaag gagatagagg taccgccaat gtcaatggtt
    12721 gtcgctttgg tattattggc tgcaagtgtg gacagactag cctcagtcaa agaagcacca
    12781 gagccgtttt tcgttaagcg cccagttgta tcaatgtaga gtttatctcc attgcttgcc
    12841 gtaattttac catcactgct gatctgaaca tcctgtgcag aaccatcgat agtaatagtg
    12901 cccttagcgg tatcaccaac tcccggggtc aaatatttct gaacatcggc agctgaagcc
    12961 gttgtggtat caaaagagta tgacttactt gcactgtcat acttgtaatt cgttgcacta
    13021 gccgccgttc cgaagccgtt attaatggtt gcctgaacag tatcaccatc actaactcca
    13081 gccagcaaat cagacgcttt agcagcatcg taacccgcac tcacagtata tttgttgccg
    13141 accactgttg cattagcagc taccaagtca gctttagatg cagcagtatt agccacagcc
    13201 ccgccaccat tcacattaaa cccattcagc cccagcgtat cagagtcaat tttcttcaga
    13261 tcaatagtga tagtctggcc gtcattcgca ccaacctgaa ttttcatcga accgtctttt
    13321 gccagtacgt tcacgccgtt gaactgggtc tgaccggata cgcggtcaat ttcgtcgaga
    13381 cgggatttga tttcgtcctg aatggagtcc agatccgaat cagagttagt cccggtagaa
    13441 gcctgaaccg tcagctcacg gatacgctgt aagttgttgt tgatttcgga cagcgcgcct
    13501 tcagtggtct gtgcaacaga aataccgtcg ttggcgttac gtgcagcctg agtcaggcct
    13561 ttaatgttag aagtaaaacg gttagcaatc gcctgacccg cggcgtcatc cttcgcgctg
    13621 ttaatacgca agccagaaga cagacgctcg atagaactcg acagcgcaga ctggttcttg
    13681 ttgatattat tttgagtgat cagcgagagg ctgttggtat taatgacttg tgccatgatt
    13741 cgttatccta tattgcaagt cgttgattac gttttgggtt tccacccgtc ggctcaatcg
    13801 ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag aatttttttc acaaacagcc
    13861 attttttgtt agtcgacgaa atactctttt ctctgcccct tattcccgct attaaaaaaa
    13921 acaattaaac gtaaactttg cgcaattcag gccgataacc ccggtattcg ttttacgtgt
    13981 cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg gtcaggtctg
    14041 gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct aacccctatt
    14101 tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct gaaaagcgcg
    14161 ctgacgactt tccagaccgc caatactgca ttgtctaaag ccgatctttt ttccgccacc
    14221 agcaccacca gcagcaccac cgcgttcagt gccaccaccg cgggtaacgc catcgccggg
    14281 aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc
    14341 agagatgata cgaaaacggc gatcgccacc agcgacagca aactcaccat tcaacaaggc
    14401 ggcgacaaag atccgattac cattgatatc agcgcggcta actcatcgtt aagcgggatc
    14461 cgtgatgcca tcaacaacgc aaaagcgggc gtaagtgcga gcatcattaa cgtgggtaac
    14521 ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgataatgc gatgacactc
    14581 tcggtcagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag tgccagcagc
    14641 aacggtatgg aggtctcggt cgccgcccag aatgcgcagc tgacggtcaa caacgtcgcc
    14701 atcgagaaca gcagcaacac tatcagcgac gcgctggaaa acatcaccct gaacctgaac
    14761 gatgtcacca cgggcaacca gacgctaacc atcactcagg acacctccaa agcgcaaacg
    14821 gcgattaaag actgggtgaa tgcctataac tcgctaatag ataccttcag cagcctgacc
    14881 aaatacaccg ccgtagatgc gggagctgat agccagagtt ctagcaatgg cgcactgctc
    14941 ggcgactcca cgctgcggac gattcagacg cagttgaagt cgatgctgag taataccgtc
    15001 agttcttcca gctataaaac gctggcgcag attggtatca cgaccgatcc cagcgatggc
    15061 aaactggaac tggatgccga caaactcacc gctgcactga aaaaagatgc cagcggcgta
    15121 ggtgcattga ttgttggcga tggtaaaaaa accggcatca caaccaccat cggcagcaac
    15181 ctgaccagtt ggctttcgac aacgggcatt attaaagccg ctaccgatgg cgttagtaaa
    15241 accctgaata agttaactaa agactacaac gccgccagcg atcgcattga tgcgcaggtc
    15301 gcgcgctaca aagaacaatt tacccaactg gacgttttaa tgacctcgtt aaacagcacc
    15361 agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc attattcatc
    15421 gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcgcaaat tggcgtcgaa
    15481 agcaccgtaa tgagcgccag ccaacagcag ttggtcacca tgctatttga tggagtgctg
    15541 agcgcactgg ttagagcgcg actgtttatg caggacaaca atcagcaagg caaaggcgtc
    15601 tctttgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct tgatgaagag
    15661 agcaaagacg aattaaccca aaacttaatt gctctttata gctatatggt caggcgtttg
    15721 ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aggtggaagc attaatgcgt
    15781 aatattgccg atgcctggaa agagtcttta ctctcccctt cttcgattca ggacccagtc
    15841 tgatgaacaa tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc
    15901 tcatgttacg cctggcaacg gaagaacaat gggatgaact catcgctagc gaaatggcgt
    15961 atgtgaatgc ggtgcaggag atcgcacacc tgactgaaga ggttgccccg tccactacga
    16021 tgcaggagca gctccgcccg atgctgcacc tgattctcga caacgaaagc aaggtaaagc
    16081 agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
    16141 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caggataacc
    16201 tcttttgaat cttaatgagc cgatggctcg cgaataatcc gattacggct acgcttctaa
    16261 tattcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
    16321 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgcag acggttttaa
    16381 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcggcta
    16441 ttcggtcggc tacgactcct atgatttatt tgatctaggt gagtttgatc agaaaggcag
    16501 catccctact aaatatggtg ataaagcaca actgctggcc gccattgacg ccctgaaacg
    16561 taatgacatt gcggtgctgc tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
    16621 agaagctatt cgcgtgcagc gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat
    16681 cattgagtgt gaaggctgga cgcgttacac cttccccgct cgtgccgggc aatactcgca
    16741 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaacacc ctgatgaaga
    16801 tggcattttt aaaattgtta acgactatac cggagaaggc tggaacgatc aggttgatga
    16861 tgaactcggt aatttcgatt atctgatggg tgagaatatc gattttcgca atcatgccgt
    16921 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggctt
    16981 tcgtcttgat gcggtcaaac atattccggc atggttttat aaagagtgga tcgaacacgt
    17041 acaggaagtt gcgccaaagc cgctgtttat tgtggctgaa tactggtcgc atgaagtcga
    17101 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgtttg atgcgccgct
    17161 gcagatgaaa ttccatgaag catcgcgcat gggtcgcgac tacgacatga cgcagatctt
    17221 cactgggacg ctggtggaag ccgatccttt tcacgccgta accctcgttg ccaaccacga
    17281 cacccaaccg ttgcaagccc tcgaagcacc ggtcgaaccg tggtttaaac cgctggcgta
    17341 tgccttaatt ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg acctctacgg
    17401 tgcacattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat
    17461 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacatt
    17521 atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg
    17581 ctgcgtggtg gtcatgtcga acggagatga tggcgaaaaa accattcatc tgggagagaa
    17641 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg tagtgacaga
    17701 cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga
    17761 agaggtgatt taaatccatc cccggcggca agccggggag atttcattac ggcagtggcg
    17821 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    17881 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    17941 gttttacggt aacgttagtg agatcatcag acatcaatgt caacttaccc ggcacggcaa
    18001 tcccccgctg ccactgacgg caatccaagg tatcgccctc tttagtgaca atcaggctgc
    18061 ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag tagcccgcca
    18121 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    18181 ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    18241 aaaagaggat tgtggcatta aagctctgat gtcgccagcc tttccaggtt cagagagaaa
    18301 tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc atggtgtatg
    18361 attcgcggct cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    18421 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    18481 tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca cgggtgaatt
    18541 tacccgttgg ggcggtcagc tcctgcagct gtttggcgtc catgctgaag agtggggtta
    18601 ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggca tgatgatcct
    18661 cggtatgttt ggcggctgct ttgctgctgc gctgtgggcc aacaatgtca aactacgcat
    18721 gccacgcagc cgtatccgca ttatgcaggc catcattggc ggcattatcg ccggttttgg
    18781 cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggcattc ctcagttctc
    18841 gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcgcgctt
    18901 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc
    18961 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    19021 cggcatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat
    19081 gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcggc
    19141 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat
    19201 ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ctaaaatcat
    19261 gtgggcggga ccaaatgcgg taattggtgg tttacttttt ggttttggca tcgtgctggc
    19321 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    19381 ggtcggtctg ggcaacgtga tcggctcaac gattctggcg tattactggg atgatttcgc
    19441 tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacttttg gtccgatggg
    19501 aggcctgctg gtgacatatt tgctgctgtt tgctgcgctg atgttgatta tcggctggga
    19561 aaaacgcttc ttccgccgtg cggcaccgca gactgctaag gagatcgcat gaaaaatatc
    19621 gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc agtcgcaacc
    19681 cttgaggcga tgccgcaatt aaaaaaaggt gagatcctgg aagtggtgag cgactgtccg
    19741 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctacactgt gctggatatt
    19801 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    19861 gcccacagcg ggaacatcgt tacctctgac acatagattt tacccgcccc ctctcctatt
    19921 cttttcagta tgatctcagg aggttgctat gtgtggacgc tgtgcccaat cccaaacgcg
    19981 tgaggattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct acgatcccga
    20041 acccattggc agatacaatg tcgcgccggg aaccaaagtt cttctgctca gtgaacgtga
    20101 tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt gggataaacc
    20161 gccgctgatt aacgcccgcg tagaaactgc ctcaaccagt cgcatgttta aaccgctctg
    20221 gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa aagaaggcga
    20281 caaaaaaagc cttattttat ctatcgcgct gacggacaac ctgtttttat ggccgcgata
    20341 ggtagcatac cgtttgagcg tggtgacgaa gccgaaggat ttttgatcgt cactgcggcg
    20401 gcagatcaag gtctggtaga tattcatgac cgccgcccgc tggttctgtc gccagaagct
    20461 gcacgggaat ggatgcggca agacattggt ggtaaagaag cctcagaaat cgctaccaga
    20521 agctgtgtgc cagcaaacca gttcacctgg caccctgtgt cgcgcacggt gggtaatgtc
    20581 aaaaaccagg gcgcggagtt aattcaacct gtttgttgac atcctccacg ccctgaatga
    20641 cgaggccccc tgctacgttc a
//
LOCUS       DADLXX010000070        20549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLXX010000070 DADLXX010000000
VERSION     DADLXX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.070"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JNX27_002406"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JNX27_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7883594.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JNX27_002407"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JNX27_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY7883595.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JNX27_002408"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JNX27_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7883596.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNX27_002409"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNX27_002409"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7883597.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNX27_002410"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNX27_002410"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7883598.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JNX27_002411"
     CDS             4449..4814
                     /locus_tag="JNX27_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7883599.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JNX27_002412"
     CDS             4964..5482
                     /locus_tag="JNX27_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY7883600.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JNX27_002413"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JNX27_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JNX27_002414"
     CDS             6713..7195
                     /locus_tag="JNX27_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7883601.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNX27_002415"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNX27_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7883602.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNX27_002416"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNX27_002416"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7883603.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNX27_002417"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNX27_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7883604.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNX27_002418"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNX27_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7883605.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNX27_002419"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNX27_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7883606.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JNX27_002420"
     CDS             10864..11730
                     /locus_tag="JNX27_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7883607.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JNX27_002421"
     CDS             11751..13037
                     /locus_tag="JNX27_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7883608.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNX27_002422"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNX27_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7883609.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JNX27_002423"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JNX27_002423"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7883610.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JNX27_002424"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JNX27_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7883611.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JNX27_002425"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JNX27_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7883612.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JNX27_002426"
     CDS             complement(16985..17275)
                     /locus_tag="JNX27_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7883613.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JNX27_002427"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JNX27_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7883614.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JNX27_002428"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JNX27_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7883615.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JNX27_002429"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JNX27_002429"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JNX27_002430"
     CDS             19137..20378
                     /locus_tag="JNX27_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7883616.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAAARGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5364 c         4827 g         5056 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgt gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcggac agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgttgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaattagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcta
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttagacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgcat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatctc tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgccagtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggctcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg caggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ctgatttag
//
LOCUS       DADLXX010000071        20494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLXX010000071 DADLXX010000000
VERSION     DADLXX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.071"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="JNX27_002431"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="JNX27_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7883617.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(476..688)
                     /gene="cspA"
                     /locus_tag="JNX27_002432"
     CDS             complement(476..688)
                     /gene="cspA"
                     /locus_tag="JNX27_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7883618.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(969..1259)
                     /locus_tag="JNX27_002433"
     CDS             complement(969..1259)
                     /locus_tag="JNX27_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY7883619.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1693..2403
                     /locus_tag="JNX27_002434"
     CDS             1693..2403
                     /locus_tag="JNX27_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7883620.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2453..3427)
                     /gene="ghrB"
                     /locus_tag="JNX27_002435"
     CDS             complement(2453..3427)
                     /gene="ghrB"
                     /locus_tag="JNX27_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7883621.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3531..4190)
                     /locus_tag="JNX27_002436"
     CDS             complement(3531..4190)
                     /locus_tag="JNX27_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7883622.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4343..6676
                     /locus_tag="JNX27_002437"
     CDS             4343..6676
                     /locus_tag="JNX27_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7883623.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6645..7085)
                     /locus_tag="JNX27_002438"
     CDS             complement(6645..7085)
                     /locus_tag="JNX27_002438"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7883624.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7082..7645)
                     /gene="tag"
                     /locus_tag="JNX27_002439"
     CDS             complement(7082..7645)
                     /gene="tag"
                     /locus_tag="JNX27_002439"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7883625.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7803..8501
                     /locus_tag="JNX27_002440"
     CDS             7803..8501
                     /locus_tag="JNX27_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7883626.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8730..9938
                     /locus_tag="JNX27_002441"
     CDS             8730..9938
                     /locus_tag="JNX27_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883627.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10262..11953
                     /gene="eptB"
                     /locus_tag="JNX27_002442"
     CDS             10262..11953
                     /gene="eptB"
                     /locus_tag="JNX27_002442"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7883628.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12045..12121
                     /locus_tag="JNX27_002443"
     tRNA            12045..12121
                     /locus_tag="JNX27_002443"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12079..12081,aa:Pro,seq:cgg)
     gene            12865..14472
                     /gene="dppA"
                     /locus_tag="JNX27_002444"
     CDS             12865..14472
                     /gene="dppA"
                     /locus_tag="JNX27_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY7883629.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14780..15799
                     /gene="dppB"
                     /locus_tag="JNX27_002445"
     CDS             14780..15799
                     /gene="dppB"
                     /locus_tag="JNX27_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7883630.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15809..16711
                     /gene="dppC"
                     /locus_tag="JNX27_002446"
     CDS             15809..16711
                     /gene="dppC"
                     /locus_tag="JNX27_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY7883631.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16722..17705
                     /gene="dppD"
                     /locus_tag="JNX27_002447"
     CDS             16722..17705
                     /gene="dppD"
                     /locus_tag="JNX27_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7883632.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17702..18706
                     /gene="dppF"
                     /locus_tag="JNX27_002448"
     CDS             17702..18706
                     /gene="dppF"
                     /locus_tag="JNX27_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7883633.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18736..20007)
                     /locus_tag="JNX27_002449"
     CDS             complement(18736..20007)
                     /locus_tag="JNX27_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7883634.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4902 a         5075 c         5389 g         5128 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaatcc ccgcaaaatg gcagggatct tagattctgt gcttttaagc agagattaca
      481 ggctggttac gttaccagct gccgggcctt tagcgccgct ttcgatggtg aaggacactt
      541 tctgaccttc gtccagagat ttgtaaccat agttctggat agcagagaag tgtacgaaca
      601 catctttaga gccatcgtca ggagtgatga agccgaagcc tttgtcagcg ttgaaccatt
      661 ttacgatacc agtcatttta ccggacatag tgtattacct ttaataatta agtgtgcctt
      721 tcggcgatat ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca ctacgagggg
      781 tatcaacgat aactcttgaa gggacttgcc ttactacact gctttaatgg tctgtacgtc
      841 aaaccgttga tgtgcattaa gccactcatt ggcgggtgat gcaacaatta tttttcatat
      901 ttatgattaa tcggttggaa aacggtcctg tcatcaggac cgtaaacagc aataaagtgg
      961 ataaaagtct atttcatcaa ctgcttactt aatgccgggt tggcttgaat caaacgcatc
     1021 aattttagtt cggcacttga aggcttcacg cgtctggatt cccattcctt taccatggcg
     1081 actgatacgc ccaaaacccg ggcgaaatca tcgattttta atcctgtccc ttttcgtaac
     1141 cgctcaattt cggtacagga cgttgttctg tgcgtcaggg taattttctg cgtttcatct
     1201 ttaaaaacaa tctgttccag gctgctcaac agctcatgca ttggatcttt atattccatt
     1261 gaaaactcct caaatcacac tgcgggatcg tgaattgcat cgaagctcat taagaatagt
     1321 cgggaaaagc agacacagcg gggttaagtc agggcatgct ctggctatga ttcgattcag
     1381 cctgtcattt agcgtgactt atagccacct aacgatctga taaatgccga tatatctctt
     1441 atcactcctg ttttgtaaag atttcgcaaa tctaaccttc tgcgaaggcg taaatctctg
     1501 gctacaaaaa aggcaacggc actatcattt tttaacaatg aaacaacaaa ttaaccttgt
     1561 ttcattttct ccatgatttt accaggaaga ttacgtagaa agggtatcct gcaagcctgt
     1621 cctggactat ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt tttgggtgaa
     1681 aggagtaaga aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc tcttgcggcg
     1741 ttattaatgg tagttagcct gagtgggtgt tttgataaag aaggcgatca gcgtaaagcg
     1801 tttatcgact tcctgcagaa tacagtgatg cgtagcggtg aacgtctacc aaccctgact
     1861 gccgatcaga aaaaacagtt tggtcctttt gtctctgatt acgcgattct gtatggttat
     1921 tctcagcagg tgaatcaggc gatggattcc ggtctgcgcc cggtcgtaga cagcgttaac
     1981 gcaattcgcg taccgcagga ttacgttacg caaagcggtc cgctgcgtga aatgaatggt
     2041 tcgctgggtg tgctggcgca acaactgcaa aatgcgaagt tgcaggccga tgctgcgcac
     2101 tctgcgctaa aacagagcga tgacctgaaa ccggtatttg atcaggcatt caccaaagtg
     2161 gtaacgacgc cagctgatgc attgcaaccg ttgattccgg cggcacaaac tttcacgcaa
     2221 caactggtga tggttggaga ctacattgct cagcaaggta ctcaggtgag ctttgtcgca
     2281 aatggcatcc agttcccgac ttcacagcag gcaagtgaat ataacaaact gattgcgcca
     2341 ttaccggcac aacatcaggc gtttaatcag gcctggacta cagcagttac tgcaacccaa
     2401 taaagagtaa aagcccgagc gattatcggg ctttacttca cgcagtcgcg gcttagtccg
     2461 cgacgtgagg attcacacag ttcttctcaa cctttccttg taacgcatca atcaaattat
     2521 ccacggcaca ggcggccatg ccataacgcg tctcatgggt ggcagatcca atatgcggta
     2581 ctgcgacgac gttggccatt gagagcaacg gcgaatctac ggacagtggc tcttgttcga
     2641 agacatccag cccggcggcg tgaatttcgc ctttctgcaa tgctgcgatc agtgcatttt
     2701 cgtcaaccac cgggccacgt ccggcattaa tgaaaatggc ggaggatttc attttggcga
     2761 attgttctgc gccaaacaga tgatgcgtct catcagttaa cggcaggatc aggcaaacga
     2821 aatctgactc ttgtaacaga gtatccaaat cgcagtagcg ggcgttgaag cgttcttctg
     2881 cttctttatg gtggcggcgc gcgttataga ggatgggcat gttgaagcca aagtgcgcac
     2941 gttgtgccag cgccatgccg atccgtccca tcccgacaat gcccagtgtt ttatggtgaa
     3001 cgtcagtgcc gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct tttacccgtt
     3061 ctgctacctc cacaacccga cgagcggtag acaacaccag cgccatcagc gtatcggcga
     3121 cggtttctgt taatacggtt ggcgtgtgca tcagcagaat ttttcgggcg gtaagcgcat
     3181 cgacatcaaa attgtcatag ccgacggaga tcgttgatgt ggcacgcagt ttcggcattt
     3241 tttccagcaa tgcggcatct acattctcgt ttgaacccag taaaccttca gcttctgcaa
     3301 aaattgctgc attttgaacg acggtttgtg ggctgaggtt tgccacctgg tgaacggtga
     3361 aatgcgcttg caggcgttgc agtaaatcat caggtaaggc tttgtagagg ataacggacg
     3421 gcttcatgct tctctccatt cactgataag gcgatcacct tagcctgaaa tatgatgaag
     3481 gaaaactgaa gaatgccagg tgatttctgc atcacctggc atgaaaggga ttacagcggg
     3541 cttaaggtaa tttctacacg gcggttttgc gccttacctt ctgcggtgct gttgctggcg
     3601 attgggttag ccgggccaag gccctgagta cggatgcggc tggcgtccac gccctgggtg
     3661 atcaacgcgc tggcaacgga atccgcacgc tgctgggaga gacgcatgtt caggtcgtga
     3721 ccccccgtgc tgtcggtata accaatcacg ttaaccgccg ttttcggata ctctttcagc
     3781 accattgcca cgccggtcag ggtgttagcg cctgccggtt tcaggggcgc gctgctgctg
     3841 tcgaaggtca cattgttcgg catattgagg ataatgttat ccccgctgcg ggttacgcta
     3901 acaccagtgc cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat gtaataaccc
     3961 acgccgccgc ccagagctgc gcctgctgct gcgccaatca gcgcgccttt accgcgatct
     4021 ttcttcgaag aagagagcgc accaataccc gcgcccacga gagagcccag acctgcgccg
     4081 atagcagatt tgcctgcttc gcgttcgccg gtgtaagggt tagttgtgca gccagatacc
     4141 gccagagcgc cactcactac ggcggcaata agataaacac gtttcttcat tgttaatcct
     4201 taataacctt tttattcttt gccacgggtt ccgtggcggg agattatgcc gcgtgaacat
     4261 gaagattatt cctgggaata ctcggaaatt tgtaagtaat atttaactgc tcaatacatc
     4321 taacctttca ggagtcttcg gtttggccaa ctcatcctca cgatattccg ttctgactgc
     4381 cgcccactgg gggcccatgc tggttgaaac cgacggcgaa accgtgttta gctcgcgtgg
     4441 cgcgttagcc acaggaatgg aaaactcctt gcagagcgcg gttcgcgacc aggttcacag
     4501 caatacgcgg gtacgatttc caatggtgcg caaaggcttt ctcgcgtcac cggaaaaccc
     4561 gcaaggcatt cgtgggcagg atgaatttgt tcgcgtgagt tgggatgagg cgctggatct
     4621 tattcaccaa caacataaac gcattcgtga ggcttatggt ccggcatcga tttttgctgg
     4681 ttcctacggc tggcgttcaa acggcgtgct gcataaggcc tcgacattat tacaacgcta
     4741 tatggcgctg gcaggcggtt ataccgggca tctgggggat tattcgaccg gtgcggcaca
     4801 ggcgatcatg ccgtatgtcg taggtggcag cgaagtttat caacagcaaa ccagttggcc
     4861 gctggtgctg gaacatagcg atgtcgtggt gttgtggagt gctaacccac tcaatacgct
     4921 gaaaattgcg tggaatgcat ccgatgagca ggggctttct tacttttccg cactgcgtga
     4981 cagtgggaaa aagctgatct gcattgatcc aatgcgatcg gaaaccgtcg atttctttgg
     5041 cgataaaatg gagtgggtgg caccgcacat gggcaccgat gttgcgctga tgctggggat
     5101 cgcccatacg ctggtggaaa atggttggca cgacgaagcg tttctggcgc attgcaccac
     5161 aggttatgcc gtcttcgcct cttatttgct gggcgagagt gacggaatag cgaaaaacgc
     5221 cgaatgggcg gcagagattt gtggtgttgg cgcagcgaaa atccgcgagc tggcggctat
     5281 tttccaccaa aataccacca tgctgatggc aggctgggga atgcagcgcc aacagtttgg
     5341 tgagcaaaaa cactggatga tcgtcacgct ggcagcaatg ttggggcaaa tcggcacacc
     5401 cggcggcggt tttggtcttt cttaccattt tgccaatggt ggtaacccca cgcggcgttc
     5461 tgcggtgctc tcttccatgc agggcagctt gccgggtggc tgcgatgcgg tggataaaat
     5521 ccctgttgcc cgcattgttg aagcactgga aaaccctggt ggcgcatatc aacacaacgg
     5581 tatgaaccga catttcccgg atattcgttt tatctggtgg gcgggcggtg ccaactttac
     5641 tcatcatcag gataccaatc gtctgatccg tgcctggcaa aaaccggagc tggtggtgat
     5701 ctctgaatgc ttctggacgg cggcggcgaa acacgcggat atcgttctgc ctgcgactac
     5761 ctcttttgag cgtaatgatc tcaccatgac cggtgattac agtaatcagc atctggtgcc
     5821 gatgaagcaa gtggtgccgc cacgctatga agcgcgtaat gattttgatg tttttgccga
     5881 gttgagtgaa cgttgggaga agggcggcta tgcgcggttt acggaaggaa aaagtcagct
     5941 gcaatggctg gaaacgtttt ataacgttgc ccggcagcgc ggggcaagcc agcaggttga
     6001 attgccgcca tttgctgagt tctggcaagc caaccagtta attgagatgc cggaaaaccc
     6061 ggacagcgag cggtttattc gcttcgctga tttttgccgc gatccgctgg cgcatccgtt
     6121 aaaaaccgcc agcggcaaga ttgaaatctt ctcacagcgt attgccgatt acggttaccc
     6181 ggattgccct gggcatccaa tgtggctgga gccggacgaa tggcagggca atgccgaacc
     6241 agaacagttg caggtacttt ctgcccatcc ggcgcaccgc ctgcacagcc agctgaatta
     6301 cagttctctg cgcgaattgt acgcggtagc aaaccgtgag cctgtcacca ttcatcctga
     6361 cgatgctcag gcgcgcggca taaccgaagg ggatatggtg cgggtgtgga actcccgtgg
     6421 gcagatcctt gctggagcgg tcattagcga gggaattaaa cctggcgtga tttgcattca
     6481 cgaaggggca tggccggatc tggatttaac cgctgacggt atttgtaaaa acggcgcagt
     6541 gaacgtgctg accaaagatc tccccagctc gcggctgggg aatggctgtg cgggtaatac
     6601 ggcgctggca tggctggaaa aatacaacgg tccggaactg acacttacag cgtttgaacc
     6661 accggccagc tcataatcca ggtgggtagt tgggtttcat cctgccatgc gcaatcgaca
     6721 atgtgaaaac cctgcgcacg gtaaaaatct atcgccggtt gatttttttg ataaacctcc
     6781 agcatcagat ggggatagcg ctgctgcaca tactgcatca gcgccttacc aataccgcgt
     6841 ctgacggcct tcggtgcgac aaacattgct gccagaaatc ggccttccat aatgctgaca
     6901 aaaccgagaa gcttaccgtc ctcttcccag acccagtttt gcgcgttggc aagataggca
     6961 tcccgcacca gcggaatgca gtcacgccag taattcgctt ttataaaggg atgcccccag
     7021 gttgtacttt ccagccacag ttcgaggatc gcggggagtt ctgaacgttg cgcttcccga
     7081 atcatggttt atttcccgga tagcaacagc agccaaccac atgatcattc accagcccac
     7141 atgcctgcat aaaggagtaa cagattgtgg tgccgacaaa cttaaaacca cattttttca
     7201 gtgccttaga tagggcgtcg gaggcggacg tagatgtggg aatttcgctc aacgttgtgg
     7261 cttgtgtcac ctgtggctga tgatttacaa acgaccagac aaagtcggga aacggttcgc
     7321 cgttctgttc catttgcagg tacgcccgcg cattaccaat aattgcctga attttccctc
     7381 gatggcggat aatcccggcg tcctgtacca gtctttcgac atcctcttcc tgcattgctg
     7441 cgaccttcac cggatcgaac tgatgaaagc aggcgcgata gttttcgcgt tttttgagga
     7501 cggtgatcca cgataaccca gcctgctgcc cttcaaggca gatcatttcg aacagttttt
     7561 tactgtcagt ttcaggcacg ccccactcat tatcatggta ggcaatataa agcgggccct
     7621 gactcaccca gccgcaacgt tccatacttt ccctcgctat gtacccgatc gtaaaaattt
     7681 cagccgcttt tcttgacgca tcggtgaaaa agtcaatatt taatacaggg ctactatgcc
     7741 cgatatcctt catacaatga caataatatt gccgaattcg gctcttccct ggagtcgtgt
     7801 taatgatcat aaaaaaaagc ggtggtcgct ggcagctaag cctgctggcg agcgtggtaa
     7861 tcagtgcctt ttttctcaac acagcttacg cctggcaaca agaatatatc gttgatacgc
     7921 aacccggaca ttccacagag cgttacacct gggatagtga tcatcaacct gattacaacg
     7981 atattttgtc gcaacgtatt caaagtagcc aaagggcgct gggactggaa gtcaatctgg
     8041 cggaagaaac tcctgtggat gtgaccagca gtatgagtat gggctggaat tttcctttat
     8101 atgaacaggt tacaaccggc ccggtcgcgg cattacatta cgatagcaca accacctcga
     8161 tgtataacga gtttggcgac agtactacca cgctgaccga tccgttatgg catgccagcg
     8221 tgagtagctt aggctggcgt gttgactccc ggcttggcga tctccgaccc tgggcgcaaa
     8281 tcagctataa ccagcaattt ggcgagaata tctggaaggc gcaatcaggc ctgagccgga
     8341 tgacggcgac aaaccagaac ggcaactggc tggatgtcac cgtaggcgct gatatgttgc
     8401 tcaatcaaaa tattgccgcc tatgctgcgc taactcaggc agaaaatacc actaataata
     8461 gcgactatct gtatacgatg ggggttagcg ccagatttta acgtaacagt cacaattgaa
     8521 accattgaat aacaacagtt gtggcgatag tgggtgctaa cttaccaaat aataaatttg
     8581 gtgaataatt gtcgcgtcat tcattcctga actaaggcat ttcattccgt tctgatggca
     8641 tttcatgccg tttttcccca agcataaaat gcacttcgtt atggttgtcg gcagagattt
     8701 ttccttttta ttactgcagg aatactgcca tgacaccttc aaattatcag cgtacccgct
     8761 ggctgacact catcggtact atcattaccc agtttgcgct ggggtcggtt tatacctgga
     8821 gcctgtttaa tggcgcgctt tctgccaagc tggatgcgcc ggtaagccag gtcgctttct
     8881 ctttcggctt gttaagtctg gggctggcaa tttcgtcttc tgttgcgggc aaattgcagg
     8941 aacgttttgg cgttaaacgc gtcaccgtgg cttccggcat tttgctggga ttaggcttct
     9001 tcctgaccgc gcattccaac aacctgatga tgctgtggtt aagcgccggt gtgctggtcg
     9061 gtctggcgga tggcgcgggt tacctgctga cgctctctaa ctgcgtgaag tggttcccgg
     9121 agcgtaaagg tctgatctcc gcgttcgcta tcggttctta tggtctgggt agcctgggtt
     9181 tcaaatttat cgacacgcag ctgctcgaaa cggtgggtct ggaaaaaacc tttgtgattt
     9241 ggggagcgat tgcgctggtg atgattgttt tcggcgcaac gttaatgaaa gacgcaccaa
     9301 aacaggaagt gaaaaccagc aatggtgtgg tggagaaaga ttacacgctg gcagagtcga
     9361 tgcgtaaacc gcagtactgg atgttagcgg taatgttcct gactgcctgc atgagcggcc
     9421 tgtacgtgat tggggtagcg aaagatatcg cccaaagtct ggcacacctt gatgtggttt
     9481 ccgcagccaa tgcagtaacg gttatttcca tcgctaacct ttcaggtcgt ctggtgctgg
     9541 gcattctgtc tgacaaaatt gcccgtatcc gtgttatcac cattggtcag gtgatttcgc
     9601 tggtgggtat ggcggccctg ctgtttgcac cattaaatgc agtgacgttc tttgcagcga
     9661 ttgcctgcgt ggcatttaac tttggcggca ctattaccgt ctttccatca ctggtcagtg
     9721 agttcttcgg cctcaataac ctggcgaaaa actacggtgt gatttacctc ggtttcggta
     9781 tcggtagcat ttttggttcg attatcgcct cactgtttgg cggtttctat gtgaccttct
     9841 acgtgatttt cgccctgctg attctgtcat tggcgctttc tacgacgatt cgtcagccag
     9901 agcagaaaat gttgcgtgag gcgcatggct ccctttaatg ctcagccgcg agctactggg
     9961 cttcagcttc cgtgtgcatc aaacgcatca acgaaatgta taaacaatga ctcgtaaaat
    10021 ttgggccagt taactggccc tttttactgc tttttgtaac gctaaagtct cttttcaaac
    10081 ttgcattttt gtaaatttgt gcttcatgca cactctttcc ccacactttt tcgctttgct
    10141 gtggtctact tattcgcgcg tgtagatttt acttatctga ctacctccgc acttttcccc
    10201 tgccgggcct gaaaagccgc taagcagggt gttatcacct gtttgtccag ggtttgtttg
    10261 catgagatac atcaaatcga ttacacagca gaagctgagc tttttgcttg caatctatat
    10321 tggccttttt atgaatggcg cggtttttta tcgccgcttc ggcagctatg cgcacgattt
    10381 taccgtctgg aaaggcgttt ctgctgttgt tgaactggcc gccaccgtac tggtgacctt
    10441 ctttttacta cgtcttctat cgctgtttgg ccgccgcagc tggcgtattc tggcgtcgct
    10501 ggtggtgctc ttttccgcag gtgccagcta ttacatgacc ttccttaatg tggtcattgg
    10561 ttatggcatc atcgcttccg tcatgaccac cgatatcgac ctgtcaaaag aagttgttgg
    10621 tctgaacttt attctctggt taatcgccgt tagtgcattg cctcttatcc ttatctggaa
    10681 taaccgctgt cgctacacct tgctccgaca actgcgaacc ccagggcagc gtattcgcag
    10741 cctggtggtc gtcgtactgg cgggtattat ggtttgggca ccgattcgtt tgctggatat
    10801 ccagcagaag aaagtggaga gggcgaccgg cgttgatttg ccgagttatg gcggtgtcgt
    10861 agcgaactct tatctgccat caaactggct ttctgcgttg gggctgtatg cctgggcgcg
    10921 ggtcgatgaa tcttccgata ataattcatt gcttaatccg gcgaagaagt ttacatacca
    10981 ggctccacaa aacgttgatg atacttatgt cgtgtttatc atcggtgaaa ccacgcgttg
    11041 ggaccatatg ggtattttcg gctatgagcg taataccacg ccgaaactgg cccaggagaa
    11101 aaatctggcg gcgttccgtg gttactcctg tgataccgca accaaactct cactgcgttg
    11161 catgtttgta cgtcaggggg gcgcggaaga taatccgcag cgcacattaa aagaacagaa
    11221 cattttcgcg gttctgaagc agttaggatt cagttctgac ctctacgcta tgcagagcga
    11281 aatgtggttc tacagcaaca cgatggcaga caacattgct tatcgtgagc agattggtgc
    11341 ggagccacgt aatcgtggca agccggtaga tgatatgttg ctggtagacg aaatgcagca
    11401 atcgctaggg cgcaacccgg atggtaagca tctgatcatt ctgcatacca aaggttcgca
    11461 ttttaactac acccagcgtt atccgcgtag cttcgcgcag tggaagccgg aatgtattgg
    11521 tgttgatagc ggctgtacca aagcgcagat gatcaactcc tatgacaact cggtgaccta
    11581 tgtggatcac tttatctcca gcgtgattga tcaggttcgc gataagaaag cgattgtgtt
    11641 ctacgcagct gaccacggtg agtcaattaa tgaacgcgag cacctgcacg gcacgccgcg
    11701 tgaactggca ccgccggagc agttccgcgt accgatgatg gtctggatgt cagataaata
    11761 tctggaaaat ccggccaatg cgcaggcgtt tgcgcagctg aaaaaagaag ccgacatgaa
    11821 agtgccacgc cgtcacgtag agctgtacga caccatcatg ggttgtcttg gctatacttc
    11881 accggatggt ggaattaacg aaaacaacaa ctggtgtcac atcccgcaga caaaagtggc
    11941 agcggctaac taagcggcct gctgactttc tcgccgatca aaaggcattt tgctattaag
    12001 ggattgacga gggcgtatct gcgcagtaag atgcgctccg cattcggtga ttggcgcagc
    12061 ctggtagcgc acttcgttcg ggacgaaggg gtcggaggtt cgaatcctct atcaccgacc
    12121 aaattcgaaa agcctgctca gcgagcaggt ttttttgcat ttgtagctca tgaggatgag
    12181 aacctccggg ggcagggagg ctcgactcga cgtaggcctg ataagacgcg ccagcgtcgc
    12241 atcaggcaac ggcaccgcac ttattgtcgg atgcggcgcg accgccttat tcgaccaaca
    12301 caccacacat tttcctgcca actttgcgcc ccatatctca ctatccatta acggatttgt
    12361 gacagaaacc attgtctttt tttatatttg cttaaatctt ttttgcgtta cttatggctg
    12421 gcgctggcat tttccgctgt gcgttttagc gttcatggta ttaatcgctc agatatccac
    12481 cttttcctca gatttttttc attaaatgca taaaacttaa tcaccaaatt ttgctaaata
    12541 ttaagctgat tgttctggtt atgttggagt agcacaaatt tctctgctgc aaatagctgt
    12601 ttgaagtttt tttaatcttt gtttgtggat tctcaccgtt ggtgtaaatc ccggttggct
    12661 gtattgacgt tttcacattc tgttgacaga ttgtaggtca cgaggggcat tttatggagg
    12721 atccgcactg ttacactgat gttaattagt acggcatccc cacctcataa cgttgacccg
    12781 accgggcaaa aaacaaaaaa ggtcaggcag cgacaaccca ctgcaaaggg ttaaaacaac
    12841 aaacatcaca attggagcag aataatgcgt atttccttga aaaagtcagg gatgctgaag
    12901 cttggtctca gcctggtggc tatgaccgtc gcagcaagtg ttcaggctaa aactctggtt
    12961 tattgctcag aaggatctcc ggaagggttt aacccgcagc tgtttacctc cggcaccacc
    13021 tatgacgcct cttccgtccc gctttataac cgcttggttg aatttaaaat cggcaccacc
    13081 gaagtgatcc cgggcctcgc tgaaaagtgg gaagtcagcg aagacggtaa aacctatacc
    13141 ttccatctgc gtaaaggtgt gaagtggcac gacaataaag aattcaaacc gacgcgcgaa
    13201 atgaacgccg atgacgtggt gttctcgttc gatcgtcaga aaaacgcgca aaacccgtac
    13261 cataaagttt ctggcggcag ctacgaatac ttcgaaggta tgggcttgcc ggagctgatc
    13321 agcgaagtga aaaaggtgga cgataacacc gttcagtttg tgctgactcg accggaagcg
    13381 ccgttcctcg ctgacctggc aatggacttc gcctctattc tgtcaaaaga atatgctgac
    13441 gcgatgatga aagccggtac accggaaaaa ctggacctca acccaatcgg aaccggtccg
    13501 ttccagttac agcagtacca aaaagattcc cgtatccgct acaaagcgtt tgatggctac
    13561 tggggcacca aaccgcagat cgatacgctg gtcttctcta ttacccctga tgcttccgtg
    13621 cgttacgcga aattgcagaa gaatgaatgc caggtgatgc cgtacccgaa cccggcagac
    13681 atcgctcgca tgaagcagga taaatccatc aacctgatgg aaatgccggg gctgaacgtc
    13741 ggctacctct cgtataacgt gcagaaaaaa ccgctggatg acgtgaaagt tcgccaggca
    13801 ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg tttatcaggg cgcgggtgta
    13861 tcagcgaaaa acctgatccc gccaaccatg tggggctata acgacgacgt tcaggattac
    13921 acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag cgggtctgga aaaaggtttc
    13981 tccatcgacc tgtgggcgat gccggtacaa cgtccgtata acccgaacgc tcgccgcatg
    14041 gcggagatga ttcaggcaga ctgggcaaaa gtgggcgtgc aggccaaaat cgtcacctac
    14101 gaatggggtg agtacctcaa gcgtgcgaaa gatggcgaac atcagacggt aatgatgggc
    14161 tggactggcg ataacgggga cccggataac ttcttcgcca ccctgttcag ctgcgccgcc
    14221 tctgaacaag gttctaacta ctcaaaatgg tgctacaaac cgtttgaaga tctgattcaa
    14281 ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac tgtacaagca agcgcaggtg
    14341 gtgatgcacg atcaggctcc ggcactgatc atcgctcact ccaccgtgtt tgaaccggta
    14401 cgcaaagaag tcaaaggcta tgtggttgat ccattaggca aacatcactt cgaaaacgtc
    14461 tctatcgaat aattaaaagc catacaagac tgatggcaaa ggcaaaactg cctgatgtgc
    14521 tccgcttatc aggcctacga aaattctgca atgtattgaa tttgcacgat tttgtaggcc
    14581 ggataaggcg ttcacgccgc atccggcata aacaaagcgc actttgtcaa caatctgtat
    14641 acccggtggc gctgtgcctc tgcgtgcagc gccatccggc agcaatactt cattctctgt
    14701 ccgacaaggg cgggaattga tttgtgagca atacagacac gcagttccag gctgcgggtc
    14761 actatagaga atccgggtta tgttgcagtt tattctccga cgtttgggac tcgtcatccc
    14821 cacgtttatc ggtattaccc ttctcacatt tgcctttgtc cacatgatcc cgggcgatcc
    14881 ggtgatgatc atggcaggcg aacgtgggat ctcccctgag cgtcacgtgc agctgctggc
    14941 tgaactcggc ttagataaac cgatgtggca gcagtatctc cattacattt ggggcgtaat
    15001 gcacggcgat ctaggcattt caatgaaaag ccgaattcca gtatgggaag agttcgtgcc
    15061 gcgctttcag gccacgctgg aacttggcgt ctgcgcgatg atttttgcta cggcagtcgg
    15121 tattccggtt ggtgtgctgg ctgcggttaa acgcggttcc attttcgatc acactgcagt
    15181 tggcctggcg ctgaccggct actcgatgcc gatcttctgg tggggcatga tgctgatcat
    15241 gctggtttcg gtgcactgga acctgacgcc cgtctccggt cgcgtgagcg atatggtgtt
    15301 cctcgatgac tccaatccgt taaccggttt tatgctaatc gacaccgcca tctggggtga
    15361 agacggcaac tttatcgatg ccgtcgccca tatgatcttg cccgccattg tgctgggtac
    15421 tattccgctg gcggtcattg tgcgtatgac acgctcctcg atgctggaag tgctgggcga
    15481 ggattacatc cgcaccgcgc gcgccaaagg gctaacccgc atgcgggtga ttatcgtcca
    15541 tgcgctgcgt aacgcgatgc tgccggtagt gaccgttatc ggcctccagg tgggaacatt
    15601 gctggcgggg gcgattctga ccgaaaccat cttctcgtgg cccggtctgg ggcgctggtt
    15661 gattgacgca ctgcaacgcc gcgactatcc ggtagtgcag ggcggcgtat tgctggtggc
    15721 gacgatgatt atcctcgtca acttgctggt cgatctgctg tacggcgtgg tgaacccgcg
    15781 tattcgtcat aagaagtaag gggacatcat gtcacaggtt actgaaaata aagtgattag
    15841 cgcaccggtg ccgatgaccc cgttacagga gttctggcac tattttaaac gcaacaaagg
    15901 cgcggtcgtc gggctggttt acgtcgccat cgtgctgttc atcgcgatct ttgccaactg
    15961 gattgcgcct tataacccgg cggatcagtt ccgcgatgcg ctgctcgccc cgccagcctg
    16021 gcaggaaggc ggcagtatgg cacacttgct gggcaccgat gacgtcggtc gtgatgtgct
    16081 gtcgcggctg atgtacggcg cgcgtttatc gctgctggtt ggctgtctgg tggtggtgtt
    16141 gtcgctgatt atgggcgtta ttctcggcct gatcgccggt tacttcggcg gcctggttga
    16201 taacattatc atgcgcgtgg tcgatatcat gctggcgctg ccaagtctgc tgttggcact
    16261 ggtgctggtg gcgattttcg gcccgtcgat tggtaacgcc gcgctggcgc tgaccttcgt
    16321 tgccttgccg cactatgtgc gcttaacccg cgccgccgtg ctggtggagg tgaatcgcga
    16381 ttacgtcacc gcctctcgcg tggcaggtgc cggggcgatg cgccagatgt ttattaacat
    16441 cttcccgaac tgccttgcgc cgctgattgt tcaggcgtcg ctcggtttct ctaacgccat
    16501 tctcgatatg gctgctcttg gtttcctcgg catgggggca cagccgccaa cgcctgagtg
    16561 gggcaccatg ctctccgacg tgttgcagtt cgcgcaaagc gcctggtggg tcgtgacctt
    16621 ccctggtctg gcgatcctgt tgacggtgct ggcatttaac ctgatgggtg acggtctgcg
    16681 tgacgcgctc gatcccaaac tgaagcagta agaggttcga gatggcgtta ttaaatgtag
    16741 ataaattatc ggtgcatttc ggcgacgaaa gcgcaccgtt ccgcgccgta gaccgcatca
    16801 gctacagcgt aaaacagggc gaagtggtcg ggattgtggg tgagtccggc tccggtaagt
    16861 cggtcagttc actggcgatt atggggctga ttgattatcc gggccgcgta atggcagaaa
    16921 aactggagtt taacggccag gatttgcagc gtatctcaga aaaagagcgc cgcaacctgg
    16981 tgggtgccga agtggcgatg atcttccagg acccgatgac cagccttaac ccatgctaca
    17041 ccgtgggttt ccagattatg gaagcgatta aagtgcatca gggcggcaac aaaagtaccc
    17101 gccgtcagcg ggcgattgac ctgctgaatc aggtcggtat tcccgatccg gcttcgcgtc
    17161 tggatgttta cccgcatcag ctttccggcg gcatgagcca gcgcgtgatg atcgccatgg
    17221 cgattgcctg tcggccaaaa ctgctgattg ccgatgaacc gaccaccgcg ctggacgtga
    17281 ccattcaggc gcaaatcatc gaactactgc tggagctaca gcagaaagag aacatggcgc
    17341 tggtgttaat tacccatgac ctggcgctgg tggcggaagc tgcgcataaa atcattgtga
    17401 tgtatgcagg tcaggtagta gaaacgggcg atgcacacgc catcttccat gcgccgcgtc
    17461 acccgtatac tcaggcattg ctgcgtgcgc tgccagaatt tgctcaggac aaagaacgtc
    17521 tggcgtcgtt gccaggtgtt gttcccggca agtatgaccg cccgaacggc tgcctgctta
    17581 acccgcgctg cccctatgcc actgacagat gtcgcgctga agaaccggcg ctgaatatgc
    17641 tcgctgatgg gcgtcagtcc aaatgccatt acccacttga tgatgccggg aggcccacac
    17701 tatgagtacg caagaggcca cctcgcaaca accgctgttg caggctatcg acctgaaaaa
    17761 acattatccg gtgaagaaag gcatgttcgt gccggaacgt ctggtgaagg cgctggacgg
    17821 cgtttcgttt aaccttgaac gtggcaaaac gctggcagta gtgggcgaat ctggctgcgg
    17881 taaatcgacc ctcggtcggt tgctgacgat gatcgaaacg cccaccggcg gcgagctgta
    17941 ttatcagggg caggatctgc tcaagcacga tccgcaggcg cagaagctgc gtcggcagaa
    18001 aatccagatc gtcttccaga atccctacgg ttcgctgaat ccgcgtaaga aagttgggca
    18061 aattctcgaa gagccgctgc ttattaacac cagcttaagc aaagaacagc gtcgggaaaa
    18121 agccctgtcg atgatggcaa aagtcggcct gaaaaccgag cactatgacc gctatccgca
    18181 catgttttcc ggcggacaac gtcagcgtat cgctattgcc cgtggtctga tgctcgaccc
    18241 ggatgtggtg attgccgatg agccggtttc tgcgctggac gtctcagtgc gtgcgcaggt
    18301 gctgaatctg atgatggatt tgcagcagga gttggggctt tcttatgtct ttatctccca
    18361 cgacctgtcg gttgtggagc acattgctga tgaagtgatg gtgatgtacc taggccgctg
    18421 cgtggagaaa ggaacgaagg accaaatctt caataacccg cgccatccgt acacccaggc
    18481 gctgctctcc gcgacgccac gcctgaaccc ggacgatcgt cgtgaacgca tcaaactcac
    18541 cggtgaactg ccaagcccgc tgaatcctcc gccgggttgc gccttcaacg cccgctgtcg
    18601 tcggcgcttc ggcccctgca cccagttgca gccgcagcta aaagactacg gaggtcaact
    18661 ggtagcttgt tttgctgttg atcaggatga aaatccgcag cgttaactca caaaaccggg
    18721 gccatgcgct ccggtttact caatcagctt caacagtggc gacaagcata gcaaaatgcc
    18781 gtaaagcaga atcagccagg ctttaaatcc gcgaagtttt tccagttgtg cgactttata
    18841 gatcaggaaa aacggaatga ggcacgacac aatgccatat aacgggcttc ccaactgaaa
    18901 gaacaccagc actgatacac gaaacgaaac ccaaatcgtc aacgtaatga cgataaaagc
    18961 gcagatcgcc agagtcagca tgcgtgagtt aattttcttg gtatcaataa ttcggcttaa
    19021 cagattgaga ataatgcctt taatagcctc gtggaaaccg agataaatgc caaagaatgc
    19081 ggtcagtacg gcaaagatat tcagcaccgt agaggtgata tgaatgatat gtccagggat
    19141 cacctgcgcg gccagtgcca gtgccgagat attttgttca aaggcagaaa cggcttcttc
    19201 gtggctaatt gagaaggtaa acgaaaaggc aaaaaacagg atcaccgcga tgagcgtgat
    19261 ataactaatt cggtgggtac gcagcgcgag ccgggttgcc agtactttat ctgcttcccg
    19321 tttacgatag gcaatattca ttgggttaag tacctgaata aataccgcag aaaagaagca
    19381 aaatggaatg gtaagcagga catcgcggaa aaagacggag gcttgcggga aggcggttat
    19441 attggcgaaa ttccagtgcg ggatcatcgc aaaaccgaac acgacaataa tccctacttt
    19501 gaccaccacc attggcccag aaatcttaaa cagtaatcgt tcaccaccag acgcaatcgc
    19561 tatcagtacg gcgaaaatag cgactttata aagtagagat tgtgaaagat cggcatcggt
    19621 taaaccgaag gttttcaggt acgaggcgct gtcgaaaacc acggagagag agtagataaa
    19681 aatcccgtgg ataatcatca aaaagtagat aaccccgagg aaaattcccc agttcttccc
    19741 caggtaatga ctgataatat cggtgtagtc attacaggaa tcgctttcag aaagggtttt
    19801 taaataaatg tcctgcacta cccaggtggc aggataagca atgatcgctg cggtaataaa
    19861 tacccaaatt cccttcaagc caatttgtac tggcatcagc acggttccgg caccaatcgc
    19921 catgcctatg cataataaaa cccagccgaa gtcgtagcgt gtaaacggca atttctgatt
    19981 gtgtttcgat agtgtgttgt gctgcataaa gtcctgtcca gaaagaagtc ggcctcgcaa
    20041 aaatgaataa acccagtttc tcttgcgcaa caggtggaga agtcgaatac gcagtatgta
    20101 aaaaatgagg caacagattc actggggagg tcgcgaggag gcgcaattct acaaaattgc
    20161 gcatttcact accagtctgg agtcagcccg gatatgaact gcaactattg caaataaaga
    20221 aattaagctg gaaggcatcg cgcaatgagg aagattgccg ggtgctgaca gtaaaaagca
    20281 atcgtaaaat cagcggcaag ccggaagggt tctggtgagg cgcaatgttg cgggggcttt
    20341 atccctggtg gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgtacct
    20401 gacaacataa cgggggctaa tcttgaacct agaccactca agaatagccg cgaaacgttg
    20461 tcattacaac acaggcggct atatgacgct cgca
//
LOCUS       DADLXX010000072        19914 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLXX010000072 DADLXX010000000
VERSION     DADLXX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.072"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..744)
                     /locus_tag="JNX27_002450"
     CDS             complement(<1..744)
                     /locus_tag="JNX27_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7883635.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            838..1035
                     /locus_tag="JNX27_002451"
     CDS             838..1035
                     /locus_tag="JNX27_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7883636.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1129..1317
                     /locus_tag="JNX27_002452"
     CDS             1129..1317
                     /locus_tag="JNX27_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7883637.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1238..1963
                     /locus_tag="JNX27_002453"
     CDS             1238..1963
                     /locus_tag="JNX27_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HAY7883638.1"
                     /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVET
                     ISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETD
                     NRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQ
                     ASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYI
                     RFLNKRARKRLNTKLFKVQPYPK"
     gene            complement(2075..2422)
                     /locus_tag="JNX27_002454"
                     /pseudo
     CDS             complement(2075..2422)
                     /locus_tag="JNX27_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNX27_002455"
                     /pseudo
     CDS             complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNX27_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4429..5487
                     /gene="citC"
                     /locus_tag="JNX27_002456"
     CDS             4429..5487
                     /gene="citC"
                     /locus_tag="JNX27_002456"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7883639.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5502..5798
                     /gene="citD"
                     /locus_tag="JNX27_002457"
     CDS             5502..5798
                     /gene="citD"
                     /locus_tag="JNX27_002457"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7883640.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5795..6703
                     /gene="citE"
                     /locus_tag="JNX27_002458"
     CDS             5795..6703
                     /gene="citE"
                     /locus_tag="JNX27_002458"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7883641.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6714..8246
                     /gene="citF"
                     /locus_tag="JNX27_002459"
     CDS             6714..8246
                     /gene="citF"
                     /locus_tag="JNX27_002459"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY7883642.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8250..8801
                     /gene="citX"
                     /locus_tag="JNX27_002460"
     CDS             8250..8801
                     /gene="citX"
                     /locus_tag="JNX27_002460"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY7883643.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8776..9654
                     /gene="citG"
                     /locus_tag="JNX27_002461"
     CDS             8776..9654
                     /gene="citG"
                     /locus_tag="JNX27_002461"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7883644.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9705..11168
                     /gene="citT"
                     /locus_tag="JNX27_002462"
     CDS             9705..11168
                     /gene="citT"
                     /locus_tag="JNX27_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY7883645.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11282..12088
                     /gene="rna"
                     /locus_tag="JNX27_002463"
     CDS             11282..12088
                     /gene="rna"
                     /locus_tag="JNX27_002463"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7883646.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12317..12727
                     /gene="rnk"
                     /locus_tag="JNX27_002464"
     CDS             12317..12727
                     /gene="rnk"
                     /locus_tag="JNX27_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7883647.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12958..14195)
                     /locus_tag="JNX27_002465"
                     /pseudo
     CDS             complement(12958..14195)
                     /locus_tag="JNX27_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14416..14844
                     /gene="uspG"
                     /locus_tag="JNX27_002466"
     CDS             14416..14844
                     /gene="uspG"
                     /locus_tag="JNX27_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7883648.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNX27_002467"
     CDS             complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNX27_002467"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7883649.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNX27_002468"
     CDS             complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNX27_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7883650.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17710..18456
                     /gene="dsbG"
                     /locus_tag="JNX27_002469"
     CDS             17710..18456
                     /gene="dsbG"
                     /locus_tag="JNX27_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7883651.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18666..19568
                     /locus_tag="JNX27_002470"
     CDS             18666..19568
                     /locus_tag="JNX27_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7883652.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19715..19914
                     /locus_tag="JNX27_002471"
                     /pseudo
     CDS             19715..19914
                     /locus_tag="JNX27_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5018 a         5127 c         4757 g         5012 t
ORIGIN      
        1 aacttgcgaa ccgcaaagcc agttcccgtt attatccatg tacgcctgac catcggtgcc
       61 attggctgtc cttgagttat taatcatgta gatgccaaat tgcctattcc ccagcccacc
      121 aattacgaat ttacggtcgg tatggtcctg gcgaagcaat gcctgcgcac catcgttgga
      181 taccgcatta cggcccataa taacgttctg gtcacgcata tgaatccaca tgcctgtact
      241 gctgttaatt aaaaaacggt ttgtaaatat atctcctgta acaaccagac catgccccat
      301 gcttatccgg ccagttctga gattaagcgt aaaggggcgt agtggcccta tattaccatt
      361 ttccccttca ttctctcgtg tagggatgat atgcaggcat tcttcagaac gacgaaaaat
      421 ggcaccaaaa gatgaattaa atattctcag tgcattgacc gtcgatattt ttacttcact
      481 gctgaaaagg gctttaacaa gaacatacag agcatcccat ttaagattca tcaggtcttt
      541 aacccggccg ccagtgaggt ggtgtctcca tttgaaatac tcattgccgt tgtcgcctgt
      601 ttcaaaccac atgtatgaat cagtatcgct gtcggcatca tttttaaatc caatctttgc
      661 ccagtcagta ttccgaatcc aggcaaggat tgagtcgttt tcaaaagtaa gtccaccgga
      721 caacgtatcg ccattttttt gcacggcgtt accagccttg tttaccgttt cctgtaaacc
      781 gaggggccgt ttctggcctg catggcatga tttccgcttt taaacgggga attcagcgtg
      841 ctgattggct atgtaagggt atcaacaaat gaccagaata cagacctgca acgaaacgct
      901 cttgtttgtg caggatgtga acaaatattt gaagataaat taagcggaac aaggacagac
      961 cgaccgggat taaaacgtgc tttaaagcgc cttcaaaaag gaacgcatat agaaaacgac
     1021 gatcgaatca attaaatcga tctatattac cgatcgatta tgtcccgata accacacccg
     1081 actgatgaga ttttttaaga taacggcgaa ttgatgcaag gaggtgaaat gaaatcaatt
     1141 cgctgtaaga actgcaacaa actgttattt aaagcggatt cctttgatca cattgaaatc
     1201 aggtgtccgc gttgcaaacg tcacatcata atgctgaatg cctgcgagca tcccacggag
     1261 aaacattgtg ggaaaagaga agaaatcacg cattctgacg aaaccgtgcg ttattgagta
     1321 tgaaggccag attgttggct atggttcaaa ggagctgcgc gttgaaacca tatcctgctg
     1381 gctggcccgc acaatcattc agacaaagca ctattcccgc cgttttgtga ataactctta
     1441 ccttcacctg ggagtattca gcgggcgtga tctggttggc gttctccagt ggggatatgc
     1501 ccttaacccc aactcaggtc gtcgtgtcgt gcttgaaacg gataaccggg gctatatgga
     1561 gttgaatcgc atgtggctac acgacgacat gccccgcaac tctgaatcac gggccatcag
     1621 ctacgcgctg aaagttatca gattactgta tccgtcagtg gagtgggttc agtcctttgc
     1681 tgacgaacgc tgcggactcg caggcgttgt gtatcaggcg tcgaattttg attttattgg
     1741 cagccatgaa agcacgttct acgagctgga tggtgaatgg tatcacgaga taacgatgaa
     1801 cgcgattaag cgcggagggc aacgaggcga gtatttgcga gctaataagg agcgtgccgt
     1861 ggtgcacaaa tttaatcagt atcgctacat cagattcctg aacaagcgag caaggaagcg
     1921 gctaaatacc aaactattca aggttcagcc ataccctaag tgatccccat gtaatgaata
     1981 aaaagcggta attaatactg ctgtttcatt tgaagcgcga aagctaaagt tttcgcattt
     2041 atcgtgaaac gctttcgcgt ttttcgtgcg ccgcttcacc cagccgttca taggcaattg
     2101 gcttaatgag ataatcaaat acaccacaac gtacggcttc agacaccgtt tccatatcac
     2161 tggctgcagt ggtaaacacc acgtcgccgg gataatgcgc ctgcaccagt tcatgcagta
     2221 aattaatccc tctaccgtca ggaagatagt tatcgagcaa gattagcccc ggcttaaaac
     2281 gctcgatcat cattcgggcc tgcgccaggt ttcccgccag taatatctga ctgaatccgg
     2341 gaatgtgacg aatatattcc gcatgcatct ctgccagcgg cgtttcgtcc tcaacgatca
     2401 atagggttaa tggagctgtc attaggtttc actttcggaa tatagattga aaataaggta
     2461 ccgcagggat cattatcttc gagagtgata acaccaccgc agcgcgttac gtagctggca
     2521 atcaagtaca acccaatgcc atgttcaccg ggctcgtcag cacgcgtact gacccctgct
     2581 caaatatttt gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg acttcaatca
     2641 ccacatcatc gccttcatcg ctgaggaata attcaacgct cttgtttcct tcatcgctac
     2701 gcaggctggc ttcgaaggcg ttatcaagta aattgccaac aatggcggca aactcggtgc
     2761 tatccagtcc tggcggcagt tgcgaaagct ggctaccggg gacgatgatc atttttagcc
     2821 ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc cgccacctgg cgatcggcaa
     2881 acgcctcgcg caggctgtca ataagctgtt gctgggcctg agactccccc tgcaccatcg
     2941 ccagcacgcg atcatactct ttcatctgca acagaccatt gagagtcgac atccaattga
     3001 gatgctcgtg tcgcaatgta cgaaggctct caacgtattg ttttatttgc gtcagttgcg
     3061 cattgagggt ggaaatttcg tctttactgc gaaagctgat aatggctccc agcaaatcat
     3121 cacctgaacg aatagcttcc cgattggcaa taacgctcag accgttaaag ttagccacca
     3181 catcctgacg tttttcatca atctgttcgg taaagaaatc ggcagacctg accacttcag
     3241 caatgggttt acccaaccat tgccgtccag gggaactcag ccccagcatc tttcttgcgt
     3301 tacgattgat ggcggtaatg taaccgtgcg gatccaccgc aatcagccct tcataaaccg
     3361 aactaaacag cgcctcttgc tgacggacca cccgtgcgat ttgctttggc tccatgccca
     3421 tcatctgccg acggatatgc gcggccagga accacgacag caacatcaga atccctaaca
     3481 gcacgacaaa cacacctgcc atcggtaata aaaactcagc ccgccagcta tcgattttac
     3541 tcaccaggta gccaatcgac accacgccga tgacttttcc atcgtcatca aagattggcg
     3601 ttttggcgcg catcgccata cccattgacc ctttcccggt aatgaagtag ctctcccctt
     3661 tctccagcgc gccctgtttg gtgaactgca taggataacc aattttctcc ggattaggat
     3721 ggtaaaggcg gatcgagtgc cggtcgccaa tcaccacata atcaaaatcg gtatctcttt
     3781 gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg cgttttcacc gcagagatga
     3841 cactgtcatt ggaggcaata attttcgcct gattcattgc catgtcgcgt acatgaagcg
     3901 ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg acaaatattg
     3961 agaacaccag aatcagcaaa aagatacgca gcggaaatgt cagtctttgg aaaaatgcaa
     4021 actgtttatt ctcgttaagc tgcaacattg ttttccttgc gggttgtccc gagcctcttt
     4081 gacggatgta attaaaatgt tgaatcaaaa tacatagata ttaattatga tcgtggcaaa
     4141 ttttattcat taaatcaatt aaatcactta aacctgcgag tcatttcgta attacattaa
     4201 ttaaaaaact taaaaccatt aaacaaataa aaaaaccact aaccctatgt gaaataaatc
     4261 aaaatttcac gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc
     4321 gatcacaccc ctttctccca agtgaaaata aaaggttatc agtttgcaac attgaacaac
     4381 attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat gttcgtcaat
     4441 gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc
     4501 ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc
     4561 gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc
     4621 agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc
     4681 gcctatgagc ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg
     4741 ttccgccagt gcggtttttc cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa
     4801 aacagcgcca cgcgactgaa acgctatgcc gaatcgctga aaaaattgcg tcatccaggg
     4861 aacaagattg gctgcattgt gatgaacgcc aatcccttta cgaatggtca ccgttatctg
     4921 attcaacaag ctgcggcaca gtgcgactgg tttcatctgt ttttagtcaa agaagattct
     4981 tcacgcttcc cctatgaaga ccggctggat ctggtgttaa aaggcaccgc cgatattcca
     5041 cgcctgactg tgcatcgcgg ctcagaatac atcatctccc gcgctacgtt cccttgctac
     5101 ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc
     5161 cgtcagtacc tcgctcccgc gcttggtgta actcaccgct ttgtcggtac tgaacccttt
     5221 tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc
     5281 tccgcagcgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata
     5341 tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg
     5401 gtccctgcag tcacgctgca ttatttgcag aacctgcttg aacactcccg ccaggacgcg
     5461 gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg
     5521 ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc
     5581 aggatatcga cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat gcaattcgca
     5641 ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg
     5701 acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca
     5761 gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg
     5821 taaaactcgc acccgccgca gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag
     5881 caactccttc atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtggcatt
     5941 acgtgaaaaa gacaccgccc gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg
     6001 cgatattgaa accattgtgc gtgtcaacgc gctggattcc gaatggggtg ttaacgacct
     6061 ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca
     6121 ggatgttctg gatattgaaa aagagatcct gcgtatagaa aaagcctgtg gtcgtgaacc
     6181 cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcagtgga
     6241 aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg
     6301 caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgcac gctgttccat
     6361 tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa
     6421 caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa
     6481 atcgctgatc aacccgcgtc agattgatct gctgcacaac ctctacgcac cgacccagaa
     6541 agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct
     6601 cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct
     6661 ggtgctctcc cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc
     6721 agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcactgaat
     6781 gcgatcttgc cgctttccag aactcaccaa agcaaaccta ccaggctgaa aaagcgcgcg
     6841 atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctcaggttta caggacggca
     6901 tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg
     6961 acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt
     7021 gccatgcgcc gctggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg
     7081 gcctacgcgg tccactggcg gaagagatct cccgtggtct gctggcggaa ccggtgcaga
     7141 tccactctca cggcggtcgt gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg
     7201 ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac accggtaaag
     7261 cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga
     7321 tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg
     7381 ttgatttgat cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtacta
     7441 cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg
     7501 tcaactctgg ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc ggcgcttcgc
     7561 tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg
     7621 ccctcggcgg tattaccgcg acgatggttg atctgcacga aaaaggtctg atccgcaaac
     7681 tgctggatgt gcagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc
     7741 acatcgaaat cagcgccaac cagtacgcta actggggttc gaaaggcgca tcggttgatc
     7801 gtctcgacgt ggtggtactg agcgcgctgg aaattgacag gcagttcaac gttaacgtgc
     7861 tgaccggttc tgacggcgta ctgcgtggcg cttccggtgg tcactgcgat accgcgattg
     7921 cctctgcgct ttccatcatc gtcgcgccac tggtacgcgg tcgtattccg actctggtgg
     7981 ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg
     8041 gtatcgcagt taacccggca cgtccggaac tagcagaacg tctgcaggaa gcgggcatta
     8101 aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc
     8161 cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg
     8221 atgttgtgca tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc
     8281 atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg
     8341 cctggctcaa gcaccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga
     8401 ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct
     8461 tagccacaaa gcagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc
     8521 cggagggcat gttgagcatt gccgccccgg ctcgcgatct caagctcgcc accattgagc
     8581 ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg
     8641 aaattctctc ccgccgcgac tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa
     8701 gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg
     8761 aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg
     8821 tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg
     8881 tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg
     8941 gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt
     9001 ggtgcctgta gtgcagaaat ggcaccggaa gcggtactcc acggattacg cccaattggt
     9061 atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggcagc
     9121 attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct caaccagccg
     9181 gttacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc
     9241 gaactgcgta ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc
     9301 cttaccggcg cacgcggtga agccgaagcg ggttatccac tggtgatcaa ccacgccttg
     9361 ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt gctcgatacc
     9421 ttgctcctac tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg
     9481 ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc
     9541 cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt
     9601 ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt
     9661 aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag
     9721 ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt cttatccctg
     9781 tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg gcaatgattg
     9841 tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg gttactattt
     9901 gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta
     9961 atgcgcaaaa acaggcgctg aaatggggcc tggctggttt ttccagcacc acggtatggc
    10021 tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca
    10081 ttgccctttt cctggtgaaa ttcatggaca aacgcacgct gacgttgggt tatgcgattg
    10141 tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta
    10201 cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccg aacgatccgt
    10261 ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt
    10321 catccatgtt tgtcaccggt gcggcaccaa acatgctggg tctggagttc gtcagcaaaa
    10381 ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta
    10441 tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca
    10501 gtgaagaagt ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacacgca
    10561 gagagtggac gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg
    10621 aagtcattaa tgctactgcg gttggtctgc tggcagtttc gctaatgctg gctctgcacg
    10681 ttgtgccgtg gaaagacatt acccgctata acagcgcatg gaacacgctg gtcaacctgg
    10741 caactctggt tgtgatggct aacggcctga ctcgttctgg ttttattgac tggttcgccg
    10801 gtaccatgag tacgcacctg gaaggattct caccaaacgc aacggtgatt gtactggttc
    10861 tggtgttcta ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc
    10921 tgccggttat tctggccgtc ggtaaaggta ttccgggcgt accaatggaa caactgtgta
    10981 tcctgctggt gctgtctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg
    11041 gggtgattat ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa
    11101 tcttcggggt gatttacatc tctatgttgc tgttggttgg ctggccgatt ctcgccatgt
    11161 ggaactaata cgttcgccaa acgctgaata aatcatcacg cccaccaggt aagcccacct
    11221 ggcgggcttt tttatgattt aatagatagt attcagtgta tgtatgagtt ccacacccat
    11281 tatgaaagca ttctgccgta acgccgcgtt gctcgcggtt tctctgcttc ccttatcttc
    11341 tgccaacgcc gtagcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc
    11401 cctctcctgg caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga
    11461 tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg tacatggtct
    11521 gtggccagga ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg
    11581 cttcggttgc gctactcgcc caatcccgaa tctaccagaa gcacgcgcca gccgaatgtg
    11641 ttcatcgccg gaaaccgggt tatcactgga aacggccgct aaactaagtg aagtcatgcc
    11701 aggagctggc ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt
    11761 tggttttgat ccggacgcat acttcggtac gatggtacgc ctgaatcaag aaattaaaga
    11821 gagcgaagcc ggaaaattcc ttgcggataa ttacggtaaa acagtgagcc gccgtgactt
    11881 tgacgccgcc tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg
    11941 ccagggtaac cctgcgtatt tgactgaaat tcagatctcg atcaaagctg acgccatcaa
    12001 cgctccgctt tctgcaaact cattcttgcc acaacctcac ccaggtaact gtggcaaaac
    12061 ctttgtgatt gataaagcgg gttattaatc ctctgattta caaaacacgc taattcgtct
    12121 tccataacca tgccgattaa gaattataca taatgcgctg cattgaggaa ttccctgaat
    12181 gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat catcatgtga gttaaaccct
    12241 cgccgcctga cggtgagggt tttcttttgg gtatgtttat ctgcgacact cgcagtaccg
    12301 acgacatgga gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg atgccgaacg
    12361 catcgatatt ctgctggagc aacccgccta tgctggtttg ccaatcgccg acgcgttaaa
    12421 cgcagagttg gatcgcgccc aaatgtgttc gccagaagag atgccacacg acgtggtgac
    12481 aatgaacagc cgggttaaat tccgcaatct tagagatggc gaagtgcgtg tgcgcacgct
    12541 ggtgtatccg gcaaaaatga ccgatagcaa tactcagctt tccgttatgg ctccggtagg
    12601 tgccgcactg ctggggctgc gcgttggcga ttccattcac tgggaacttc cgggcggcgt
    12661 tgcaacccac cttgaagtgc tggaactcga ataccagcca gaagctgctg gcgactacct
    12721 gctttaatcc catctgcaca agcgataact cgctttgccc agaggatgca ctcgcatcct
    12781 ctttacgcaa aatcagcaat ttttcctggt tctcactgtt cacttttgct gcaaaattat
    12841 tccgtttcgt ttgtgggaat aaccatggca gaagcatttt acatcctgat aggatttctg
    12901 attatggcgg cgattatcgt catggctgtc ctttacctcg aaaaccactc ctgacgatca
    12961 tattgttccc cccggcatcg cattcaccag acctgaaacc gccttctgcg ccgcctctgc
    13021 gctttgtgca ccgggtacca gaatcacctt acggcactcc tcttcacgtt tttcgaaaat
    13081 ctcatatccc cgggcggcct cttcaaacgg catatagtgg gtaacaattt cttctggttt
    13141 cagtaatcct ttctcaatta acggtaataa ttctcccagc catgcgtgaa cgtgggtctg
    13201 tcccatttta aacgtcaacc ctttatcaaa ggcgtcgcca aacaggaaac cgtgaataaa
    13261 tccagcgtag acgcccggta cgctaacaat gccgccacgc ctgaccgccg caatacactg
    13321 gcgcaacgct ttaccgctgc tgccctccag tttcaggtta gtcagcaccg tttccgtggt
    13381 gctgcctttc gcttcaaaac cgacggcgtc tattactgca tccacgcccc ggtgacctgc
    13441 cgtttgttca ataattgact gtgccggatc gctatcttca tcgaaattaa tcgggatcgc
    13501 gccgtagcgg tcagcggcga aacgcaagcg gtagggatga tgatcaacaa caaaaatctg
    13561 ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat cccacaggac cagcaccata
    13621 gactgcaacg cttgaacctt gttggatctg cacatttttt gctgcctgcc atgccgttgg
    13681 cagaatatca gaaaggaaaa gggctttatc atctgaaagc aaaggcggta ctttaaacgg
    13741 ccccacattc cctttaggga cgcggacata ttccgcctgc ccgccaggaa cgccaccata
    13801 caggtgacta taaccaaaca atgccgcggg agcaggtatc tgttttttat tgagcgcagc
    13861 gcctttaccc gcattggtat tttcgcaggc ggcatattgc tgtaatcgac agaaaaaaca
    13921 gtcgccacaa gcaatgacga acggaattac cacccggtcg cctttttgca aattttttac
    13981 gtccttcccg gtttcaacga cttccccata aattcatgac caaaaatatc gccatgttta
    14041 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttaccgta
    14101 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
    14161 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
    14221 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    14281 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    14341 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa
    14401 cagggagaaa gggttatgta taaggcaatc attatgccag ttgatgtatt tgaaatggaa
    14461 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    14521 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    14581 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    14641 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    14701 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttatcgg ttctcgcaac
    14761 ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg ccacgccaat
    14821 ctgcctgtac tggtcgttcg ctaacagata aaagtattta tgccgccctg cctggaacat
    14881 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    14941 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    15001 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    15061 cgtacagtca cccgctgcga acacgccttt gacgttggtt tcacatttcg catcaatgat
    15121 aatctcgccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcgggagcag
    15181 accaatctgg acaaaaatac cggccagttc gatgttgtga atattgccgc tgacacgatc
    15241 tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt
    15301 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt
    15361 catttctggc gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcgattgc
    15421 cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg
    15481 gccgtcgcag tgcgggcagt aggtcacgcc tttagtgcga tactgatctt cgcccggaac
    15541 gttcatgttg cgccattttg cacccgtcgc cacgataatg ctgcgtgctt tcagtaccgc
    15601 gccagaagct gtttcaatct ggtgtagacc accttcaacc gctgccggga tcagtttgct
    15661 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    15721 tgccaacttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    15781 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    15841 gtaaattgct gccgctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc
    15901 acgcttgttc agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat
    15961 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    16021 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    16081 cttgatgcgc gggttcaata cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    16141 atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    16201 ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac
    16261 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggtccct ggttggaacc
    16321 tgggttggtg atcaggaaag acggcttacg cactggcaag ctgttatctt ctttaaaggt
    16381 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    16441 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    16501 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    16561 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    16621 ggtgccgcag cacccgaaga attaggtgaa tttccgaagt atttcgcgtc agagcaaggc
    16681 gactagcctg tgaatccccg ggagcttaca caagtaagtg accggggtga gcgggcgacg
    16741 ccaacgccgc tatggcgtga aagacgaagg aaatttagat tttaccaacc aggtccagag
    16801 acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag
    16861 aagctacgta ctgagctgct ttgattttac gcagcagatc agacgcgtca cggccaatgc
    16921 cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac
    16981 ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg
    17041 ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt
    17101 tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt
    17161 cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg
    17221 ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt
    17281 cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct
    17341 atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat
    17401 gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga
    17461 actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa
    17521 tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa
    17581 atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag
    17641 ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag
    17701 aacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca
    17761 gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaacg
    17821 ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc
    17881 atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt
    17941 gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg
    18001 tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt
    18061 gtctacgtct tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc
    18121 ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca
    18181 gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa
    18241 caatatgaag cctctggttg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag
    18301 caaatgaaag tgttaagtga caatgagaaa ctgatggacg atctgggggc aaatgtcacg
    18361 ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat
    18421 cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg atggttgaac
    18481 tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg
    18541 ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat
    18601 acatacgcaa aaaacattta ttaagtgaat ataccatgga agaaaaatat aaaccggagt
    18661 agtgtatggc caatctctac gacttgaaaa agttcgactt aaatcttctt gtcatatttg
    18721 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
    18781 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
    18841 gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga
    18901 aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
    18961 actttattat ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
    19021 gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
    19081 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
    19141 tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta
    19201 ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca
    19261 cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt
    19321 ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg
    19381 cagttacaga tttactggga atagtcccct atgaacttta taactaccac cgtgactttc
    19441 ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct
    19501 ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga
    19561 gtttttgata tataactaca gacagctact ataactttca tctatttatt cacagctcga
    19621 atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac
    19681 tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc
    19741 actcaatatt ctggaagctg cacgagagcg tattacctag acactcaaca ccctaccccg
    19801 cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga
    19861 acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aggc
//
LOCUS       DADLXX010000073        19906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLXX010000073 DADLXX010000000
VERSION     DADLXX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.073"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            7..375
                     /locus_tag="JNX27_002472"
     CDS             7..375
                     /locus_tag="JNX27_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883653.1"
                     /translation="MFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSER
                     PIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JNX27_002473"
     CDS             complement(411..1367)
                     /locus_tag="JNX27_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7883654.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNX27_002474"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNX27_002474"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7883655.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQCEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JNX27_002475"
     CDS             complement(2605..3366)
                     /locus_tag="JNX27_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7883656.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JNX27_002476"
     CDS             3499..3909
                     /locus_tag="JNX27_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883657.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JNX27_002477"
     CDS             complement(3871..4977)
                     /locus_tag="JNX27_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7883658.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JNX27_002478"
     CDS             complement(4988..6121)
                     /locus_tag="JNX27_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7883659.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JNX27_002479"
     CDS             complement(6114..7850)
                     /locus_tag="JNX27_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7883660.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNX27_002480"
     CDS             complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNX27_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7883661.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNX27_002481"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNX27_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7883662.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEETERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="JNX27_002482"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="JNX27_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNX27_002483"
                     /pseudo
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNX27_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JNX27_002484"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JNX27_002484"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7883663.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14883
                     /gene="ybiB"
                     /locus_tag="JNX27_002485"
     CDS             13915..14883
                     /gene="ybiB"
                     /locus_tag="JNX27_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7883664.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPL
                     LAILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPV
                     GAFCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFS
                     DIGGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETT
                     AQWTERCLAGSEAIPESLKIQMACCLVATGEVATISDGLARVNQAF"
     gene            15024..16109
                     /locus_tag="JNX27_002486"
     CDS             15024..16109
                     /locus_tag="JNX27_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7883665.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16338..16598)
                     /locus_tag="JNX27_002487"
     CDS             complement(16338..16598)
                     /locus_tag="JNX27_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7883666.1"
                     /translation="MKTINTVVAAMALSTLSFAVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16890..17129)
                     /locus_tag="JNX27_002488"
     CDS             complement(16890..17129)
                     /locus_tag="JNX27_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HAY7883667.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNR"
     gene            complement(17203..17916)
                     /gene="ybiX"
                     /locus_tag="JNX27_002489"
     CDS             complement(17203..17916)
                     /gene="ybiX"
                     /locus_tag="JNX27_002489"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7883668.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(17922..19906)
                     /locus_tag="JNX27_002490"
                     /pseudo
     CDS             complement(17922..19906)
                     /locus_tag="JNX27_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4618 a         5226 c         5378 g         4684 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggtgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gcactgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gtttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggctagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aaggggaagc agagatgctc
    14101 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
    14161 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta
    14221 ttggcgattc tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat
    14281 ccaactcgcg tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac
    14341 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg
    14401 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
    14461 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc
    14521 agcgtttcgc atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg
    14581 tgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg
    14641 cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct
    14701 ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt
    14761 tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
    14821 gtggctacgg gtgaagtggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
    14881 taatttttat ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa
    14941 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
    15001 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
    15061 acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
    15121 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga
    15181 tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa
    15241 tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg
    15301 cggcacacga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg
    15361 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag
    15421 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt
    15481 tccacggtcg cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag
    15541 ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg
    15601 tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc
    15661 cgacgaccaa tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg
    15721 aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg
    15781 gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga
    15841 ccactatcat catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag
    15901 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg
    15961 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
    16021 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc
    16081 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
    16141 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
    16201 gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
    16261 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc
    16321 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
    16381 gtggcggaag tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc
    16441 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
    16501 ttctgtgcct gggatgccgt taccggttcc gccgcgaaca cggcaaatga cagagttgaa
    16561 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
    16621 tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata
    16681 aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa catacagata
    16741 aaccatttat ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt
    16801 taaaggcacg gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt
    16861 agttaacgta actggctgtc tttcgaacct cagcgattat atcctgtata cgctggtttt
    16921 tgtaaatctt tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga
    16981 cgggcttgcg ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga
    17041 atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg
    17101 gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt
    17161 taagtgtagc taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc
    17221 ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg
    17281 gcttttcagc gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc
    17341 gcggatcatc gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt
    17401 cacgcaatgc aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg
    17461 atgttgtcca aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct
    17521 taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac
    17581 tgcgccatcc acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt
    17641 ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag
    17701 cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt
    17761 aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag
    17821 ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat
    17881 acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct
    17941 gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag
    18001 gcgacgtaat cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta
    18061 acccgatacc ccagtttggc atctgcgacc cagtaacctt ctgtaaatgc tggcgttccc
    18121 accgcgccgt ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag
    18181 atatcatcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc
    18241 agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg
    18301 gtatagccgc caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag
    18361 ccttcgacgc gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca
    18421 ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc
    18481 catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca
    18541 ctgttaccac tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa
    18601 acggcatagt taatatagac attgccgttt tccgtcaggt gatacagcgc cccggctttc
    18661 cagttcacca gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc
    18721 acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggtggt ggcactgtca
    18781 tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt
    18841 tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg
    18901 ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt
    18961 aacgtcaccg gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta
    19021 ctgacatcat gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg
    19081 agaattttat tactcacatc tttgatattc gccgtgcgtg actaggtcca gctattcaca
    19141 tcgctggtgg gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc
    19201 tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc
    19261 tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa
    19321 aagttatgag tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca
    19381 gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca
    19441 tgcagataat taagatacaa acgattcgct gtaccaaggc caaaagcgac agaaggggcg
    19501 acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc
    19561 acattcaggc gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg
    19621 cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc
    19681 ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc
    19741 cctttaatca cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg
    19801 cgaataccat caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg
    19861 ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DADLXX010000074        19418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLXX010000074 DADLXX010000000
VERSION     DADLXX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.074"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..352
                     /locus_tag="JNX27_002491"
     CDS             <1..352
                     /locus_tag="JNX27_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7883669.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            509..1258
                     /locus_tag="JNX27_002492"
     CDS             509..1258
                     /locus_tag="JNX27_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883670.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1280..1846
                     /gene="yieF"
                     /locus_tag="JNX27_002493"
     CDS             1280..1846
                     /gene="yieF"
                     /locus_tag="JNX27_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY7883671.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1900..3237)
                     /locus_tag="JNX27_002494"
     CDS             complement(1900..3237)
                     /locus_tag="JNX27_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7883672.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLANEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3401..4066
                     /gene="yieH"
                     /locus_tag="JNX27_002495"
     CDS             3401..4066
                     /gene="yieH"
                     /locus_tag="JNX27_002495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY7883673.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4133..4606
                     /locus_tag="JNX27_002496"
     CDS             4133..4606
                     /locus_tag="JNX27_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY7883674.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4655..5242
                     /locus_tag="JNX27_002497"
     CDS             4655..5242
                     /locus_tag="JNX27_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883675.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5304..6026)
                     /locus_tag="JNX27_002498"
     CDS             complement(5304..6026)
                     /locus_tag="JNX27_002498"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7883676.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6028..7203)
                     /locus_tag="JNX27_002499"
     CDS             complement(6028..7203)
                     /locus_tag="JNX27_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY7883677.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7230..8846)
                     /locus_tag="JNX27_002500"
     CDS             complement(7230..8846)
                     /locus_tag="JNX27_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY7883678.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVALENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8932..10329)
                     /locus_tag="JNX27_002501"
     CDS             complement(8932..10329)
                     /locus_tag="JNX27_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY7883679.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="JNX27_002502"
                     /pseudo
     CDS             complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="JNX27_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="JNX27_002503"
     CDS             complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="JNX27_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY7883680.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMCLAINIERVRK
                     EH"
     gene            complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="JNX27_002504"
     CDS             complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="JNX27_002504"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7883681.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
     gene            complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="JNX27_002505"
     CDS             complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="JNX27_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7883682.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="JNX27_002506"
     CDS             complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="JNX27_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7883683.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="JNX27_002507"
     CDS             complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="JNX27_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7883684.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="JNX27_002508"
     CDS             complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="JNX27_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7883685.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(18307..19380)
                     /locus_tag="JNX27_002509"
     CDS             complement(18307..19380)
                     /locus_tag="JNX27_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7883686.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
BASE COUNT         5061 a         4926 c         4507 g         4924 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg gcgctggaca atgtgttaca
       61 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
      121 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
      181 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
      241 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
      301 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
      361 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
      421 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
      481 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
      541 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
      601 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
      661 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
      721 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
      781 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
      841 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
      901 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt
      961 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     1021 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     1081 cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     1141 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     1201 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     1261 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     1321 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     1381 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     1441 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     1501 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     1561 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     1621 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     1681 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     1741 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     1801 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     1861 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
     1921 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     1981 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
     2041 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     2101 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
     2161 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
     2221 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     2281 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     2341 actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat tcgccagacc
     2401 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa
     2461 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     2521 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     2581 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     2641 ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag
     2701 attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc
     2761 catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat
     2821 gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc
     2881 ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa
     2941 agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa
     3001 tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt
     3061 gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag
     3121 gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt
     3181 aaaaacgcgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat
     3241 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc
     3301 gcgtttgtga gagacttttt attggattta cttatacggc aacgattgcg ttgcgcaaat
     3361 cggctctacg aaaacgttaa actgattaaa aaggaaaggc atgtcccgga tagaagcggt
     3421 atttttcgac tgcgacggta cgctggtcga cagtgaagtc atttgctccc gcgcatatgt
     3481 aacgatgttt caggaatttg gtattacgct cgatcctgaa gaggtattca aacgtttcaa
     3541 aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt gaacatggtg ttacgttagc
     3601 gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct cggctgttcg attcagaact
     3661 ggaagccatc gaaggggctg gagcgctcct gtcagcgatc actgcgccaa tgtgtgtggt
     3721 atctaacggc ccaaataaca aaatgcagca ttctatgggc aagctgaata tgttgcacta
     3781 cttcccggat aaactgttca gcggctacga tattcagcgc tggaagccag acccggcgtt
     3841 aatgttccat gcggcaaaag cgatgaatgt aaatgtagaa aactgcattc tggttgatga
     3901 ctcagttgcc ggtgcgcaat ctggtatcga cgcaggtatg gaagtgttct acttctgcgc
     3961 cgacccgcac aataagccga tcgttcaccc gaaagtcacc acctttaccc atctttcgca
     4021 gttaccggaa ctgtggaaag cgcgtggttg ggatattacg gcatagttct tcacactccc
     4081 ttcacttacc ccgcttaaat tggcgctcaa aggtaagtaa agggagtttg atatgtctgt
     4141 ttcacgtcgg gtaatacatc acggacttta ttttgcggtt ttaggaccgt taattggtgt
     4201 tctgtttctt gtcctctaca tattcttcgc aaaagaaccg ctggttcttt tggtaataat
     4261 acaggtactt ccattgtttc tcttattgtc gattactacg ggagctattc ctgcattgtt
     4321 aaccggcgtg atggtcgcct gtctgccgga aaagatcggg tcacagaaaa attatcgttg
     4381 tctggctggt ggcataggtg gcgtcgttat caccgagatc tattgtgcag ttattgtaca
     4441 tattaagggc atggcttcct cggagttgtt tgaaaacatt ctttctggtg acagtctcgt
     4501 tgtccgcatc attcctgcat tgctggcagg tgtggtgatg agcagaatca ttacccgtct
     4561 gcccggattg gatatttcat gtcctgaaac agactcttta agttaagcgg gatactttat
     4621 ctttgggcta ctcaaaagca gacaggatgt tactatgact caaaatatca ggccgttacc
     4681 ccaattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg ataaaaccgt
     4741 agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct tttattgcgt
     4801 tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg ctgaaaagtt
     4861 tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt atgatgaaga
     4921 ggacgaattt gccggtatga agaacacata tcctgatgaa atgctgaaag agttggttga
     4981 acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt gtggcgattt
     5041 ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg atgaatttgc
     5101 caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag ctaaatgcct
     5161 gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg gcaagctgag
     5221 actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag cgtaattcat
     5281 acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc ggctttatca
     5341 gcgattacca tcagcgaagg atgcagctgc aacacggaag caggaacatc ttcggtaacc
     5401 ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac aattatcagc
     5461 aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc aggcactaac
     5521 gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc ctggatcggg
     5581 aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca aaaatgacca
     5641 tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag tttctgatca
     5701 tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat ccccgcaggg
     5761 gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt gccgcgaaag
     5821 ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc tttaaccagg
     5881 gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc caggttaaca
     5941 cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct catttcctgg
     6001 taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct gtacgaaggc
     6061 tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt cctggtaatc
     6121 aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct tgccggttac
     6181 gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa tagctgccgg
     6241 atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa caccactgaa
     6301 tgtggccagc cagccaaagc tttccagatg attcattccg gaaaccagcg cctggtaccc
     6361 gccttgtgaa agccctgcca gcgcgcgacc atcggcatct ttacggacac taaaacgctt
     6421 gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag cattcagcgg
     6481 ataaaagact ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc
     6541 ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag ccagcaggtt
     6601 atccatgatt tgcgggatac gcccctgatc gatagcggaa cgtccagtat caccaaaacc
     6661 gtgatagaaa tagagcactg gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca
     6721 gacatacatc tgacgttcag attgcaatgc gttggagtgg taagttatgg cgatcaaatc
     6781 accatgcgca acagaacgcg tatccagata actgcctagc accagaatca tactggagtt
     6841 aacctggcgc tgaggcttag tcattgcggt gcctgtatca atgctgcgta caccatcaac
     6901 attgaaaaaa tactcgtaca ggttgccttt caggataggt gtgcgccacg accagactcc
     6961 tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca ccactacaga
     7021 gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg cattgacctg
     7081 agtcacatac tgtttcaccg ggatagtggg tgccgggctg gctggcatat ccgctgctat
     7141 ggcccactga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa tttttatatt
     7201 catcgtaata ttcctttcat taagttccat taccaccaga tttcagcctg ggcaccgaca
     7261 gcaaactggt cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt ttccagggct
     7321 ttgatatacg tggcgtagaa acgaatttcc agacgcgagg tcaacatact ggtattgact
     7381 ttaaaggtat ggaagagcgt ggttttataa ccagactcat taaatttatt actattcgca
     7441 tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata ttggtcccaa
     7501 atataggccg ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg
     7561 tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg
     7621 ccgatatagg cttcgccctg cgaggtcaga cgaactgctg ttccgccggt gtgatcacca
     7681 taataacgac cattaaaggt ggtaaatgga cttgcgccag cataacggcc aaagttcctg
     7741 gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccttt atcaaatttc
     7801 tgcgttaaag atgtgccgaa catccaggta tctttccagt cataataccc gttattatcc
     7861 tgattatctt tttccgatgc gctttcgttt gccgtgacat aacgaccact caccattaag
     7921 gtcgctttat cccataacgg gatatcttta tagcgtaaat caatagtatg ggtattaatc
     7981 tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata
     8041 tcaattttac ccggaccgac tttccagttt tccagagcta cacccgctgc ggcatcagta
     8101 cgctgcgttt tccagtccag catctggatt tcaattttcg gcgcaccgtg tttacccacc
     8161 cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacgta catatcggag
     8221 aactgcataa agttctcgcc accggcgtta tcgccaaacc agccggtaga gtactgctga
     8281 ccaacgttac catccatcat cacaacggca tcaacccgtt tgccgttttc gttgtagaca
     8341 cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg gcccagagaa
     8401 ccaatcgccc atgatttagg tgaaccatga gaggcggtcc cccaaccaga acggtaatag
     8461 ccgttgtaac taaagccaat ttcatctttc acaaacttac tgaaatcttt cagagtcatg
     8521 gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttgac cagtacagca
     8581 tcaggttttg cggcactact ggtcgggaac ggattggcgg catacccttg atcattcgta
     8641 cttacgctac gattcaccgt cgctggcgta tattttttct tctcttcatc tttatacttt
     8701 ttgagttcgc tttgcgtttc tcttaacgct ttttctaata actcaaggcg ttgttccacc
     8761 gttaatgatt cagccaatga ttgtgcagaa aaggctaacg gtgccattaa taagatggca
     8821 gaggtaataa gatttcgtct aaacataata tccctttatg gtgcaaagaa agaattaacg
     8881 tatcgcatcc agactgttct gaatgcgacg ataattaagg tgctttattg gttatttttt
     8941 taatgacagc ccccgcgttt tgatcacttc tgcataccat ccgaagcttt tcttacgcgt
     9001 tcttgtgagg cttccttcgc cattatcatc acgatccaca taaataaagc cgtagcgctt
     9061 agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg tgtaccccat
     9121 aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc
     9181 aattcgataa tcatcctgta tcgaaccatc cgcttcaacg ctgtctttta cgcctaatcc
     9241 attctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta acagaacccg
     9301 taatccaacc ggatcaattt gccaccccct ctctgaactt ttcagatgcg gattggggat
     9361 catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg aaacacaacc
     9421 agtcatgtaa taactaaaag agatgaaatc tacagtatgt tttaaatctt ctgcgtcact
     9481 ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat agccgggata
     9541 ctggccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt ccatggcctg
     9601 caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc
     9661 gattttcgct tcggggatca ggctatgata ggctttaact gcccgcgcac tggcaaccag
     9721 ttgatgatgg atcgcctgat aaacttccgc ctcgccactc tcttctgcca gccccacgcc
     9781 cgtgaatggc gcgtgtaacg acatattgat ttcattaaac gtcagccata acgctacttt
     9841 atgttggtag cgggtaaaga ccgtgcgggc gtaatgctcg aagtgatcga tgaccgcccg
     9901 attagcccaa ccgccgtagt ttttcaccag cccatatagc atttcgtaat gggataacgt
     9961 taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa
    10021 ccccgcttca ttcggttcgg cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga
    10081 aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg ggtaacggtg
    10141 ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg gttccatttt
    10201 tcccattacg ccatgaggct gtaaatctga ggtcgtgatc cctttgccat cttccagcca
    10261 ggcaccttcc acctgattgg cagctgttgc gccaccccaa agaaatgttt ctggaaatgc
    10321 tttcgtaatt aactcctttt atcgttagcg aatgatggat aacagcggtt cacctgcgct
    10381 tatctgcgcc gtgccgtggg gtaatacgtc tgtaaaatca tcgctattac tgattaatac
    10441 cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag aaatcagccg
    10501 atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag
    10561 ttttacggtg tcgataccga catgaatcag gatctccaca ccatcatctg actcaatgcc
    10621 aatggcgtgt aatgtggcga acaacgaagc aattcgaccc gcaaccggag aacgcacttc
    10681 accaaccgag ggcagaatgg cgataccttt acccagcagg ccactggcaa acgtggtatc
    10741 agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac cgccctgctc
    10801 aggtggtgta ataacctctg gtgttttctc ttgcggggca ccctgcgctg gctaacgttt
    10861 actggtgatg aaatgaagca tcaacgtacc aacaaatgcg caacctatgg caatgacacc
    10921 gccaataacg ctggcccaga cggtgaaatc aatccccgtt gacgggatgg tctgcatgaa
    10981 ggtgaaaata cttggcaaac caaaggagta aactttcgtt tgcgcgtagc caatgatggt
    11041 ggcccccaat gctccactga tacaggcgat aacaaagggg tacttacgcg gcaggttgac
    11101 gccatatacc gctggttcgg tgataccaaa cagacccgtc aacgccgctg atcccgccac
    11161 cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacctgcgc
    11221 cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt
    11281 gatacacagc ggcaccaggc cccagtgcag tccgaacatg acgaagattt gccagaagcc
    11341 gcccattacc gcgcccgcaa atgcaggaac cgcctgataa agccagagat aaccggcggc
    11401 aatcatttcg cttatccagg ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat
    11461 aaccatcaga catagcaatg gtgtgaagaa atttttgatt gccgacggta accacgcatt
    11521 aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa taaccgatga
    11581 cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccggcg catccgcttt
    11641 ttgcccgttc tcgaaggcag tcagaattaa tggatgcact aacgctccac caatcaccat
    11701 ggcagtaaat ggattaccgc cgaagcgttt gcccgcggtg tatcccagga ttatcgggaa
    11761 gaacccaaac aaggcatcac tggcgctgaa taaaattaaa taagtaccac tttgttcggt
    11821 cgtccactga aaagtgagcg ccagagccag catacctttc aggatcccgg ttgccgccat
    11881 cagaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt ttagcagatt
    11941 acctttctca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt
    12001 aaccgccagg aagacatcgg ccacatggtt acctatgacc acctgaaact gaccaccact
    12061 ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg ctttgctttc
    12121 atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa tgttatctgc
    12181 gcccccgact cctgcgacta tttttctggc taactccgtc ataacttgcc ctctaccgct
    12241 ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt
    12301 ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata atatttcagt
    12361 gttctttgcg cacgcgctct atatttatgg ctaaacacat aatctctgcg ggtgaaattt
    12421 tacgttgata ctgtaaacca ataaaaatgg cgatccgttc cgcacattgc catgcttgcg
    12481 ggtaattttg ttttactgct tgttgtaatg attcatcact atcgttaatt gaagcatgtt
    12541 caagaatacg ccaggataaa aacttcagat gtgtaaccag tcgctgataa ctcaagcttt
    12601 cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg cgcattaact
    12661 gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc agatgcatgg
    12721 caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca ataatggtta
    12781 acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc cacagcaacg
    12841 ggttgggcag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
    12901 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataagac
    12961 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
    13021 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
    13081 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
    13141 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
    13201 ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg tcaggttttg
    13261 cctgcgaatg cagtaacaat ccagtcattt attaatggtt tttataacga acatccaggt
    13321 tcggaaatta atttaattgc gtgcagagaa aatagcaatg cgctattgat aaaaatatga
    13381 ccatgctcgc agttattaac tttgtgtaat tttaggaatt tataaagtta tatataacaa
    13441 atcccaataa ttaagttatt gggatttgtc tggtgaatta tttgtcgcta tcttttcccg
    13501 ccagcagttt atccagctca tcactgccta catgacggaa atcctgaccc ttcacgtagt
    13561 agaagataaa ctcacaaata ttctggcagc ggtcgccgat acgttcgatt gaacgcgcac
    13621 agaacagcgc ggtaagtacg ctcggaatag tgcgcgaatc ttccatcatg taggtcatca
    13681 gttgacgaac aataccttcg tattcctgat cgactttttt atcttcacga taaatacgta
    13741 ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg
    13801 tatgacggcc cagcgactcc agacttacca gcaacggctg atgctgctgg gagaatttct
    13861 ccagcgcagt acggcagatt ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg
    13921 ttttactgat caccataacc agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa
    13981 tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt gacgttcttg tcgccttcga
    14041 tcacgcgctt cgccagatcg ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct
    14101 gctgctccac catgccgccc atggtcatca cctgcgtgcg gatactttcc agttcggcgt
    14161 tgaactggcc ggaaatatgt ttattaagat tgagactgtc cataacgcac tcctgaatca
    14221 accgtaacga ccggtgatgt agtcttctgt ttgtttcttc gctggcttgg tgaacagatc
    14281 gtccgtgttg ctgaactcaa tcaattcgcc caggtacata aatgccgtgt ggtcggaaca
    14341 acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc
    14401 ggtgatcagc tcttcaatac gcccggtaga gatggggtcg agcgccgaac acggttcgtc
    14461 gagcagcagc acttccgggc gaatggcgat accacgcgca atacacagac gctgttgctg
    14521 accaccagag agagagtaac cgctctggtg caatttatct ttggtttcgt tccacaatgc
    14581 ggctttggtc aatgcccact gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa
    14641 cagacgaacg ccaaaggcga tgttgtcgta gatggacatc gggaacggcg tcggtttctg
    14701 gaacaccatg cccactttcg cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt
    14761 gtcgccatca agcagaattt caccttccgc acgctgctcc gggtacagtt caaacatttt
    14821 gttgaaggta cgcagcagcg tcgatttacc gcagccggac ggcccgataa acgccgtaac
    14881 ctggtttttg gcgatatcca ggttgatgtt tttcagggca tggaatttgc cgtagtagaa
    14941 gttcaaatta cgaacctgaa ttttactcgg ggcagtttca accatactca ttgcaatctt
    15001 ttcctcatcg tacctgatgc ttttcgctaa tcaggcttac cgtagggccg ggtaaggcgt
    15061 tcacgccgca tccggcaaag tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca
    15121 gaatgttcag cagcaggacg cacagggtaa tgatcaatac cccggcccag gccagttgtt
    15181 gccattccgc aaacgggctc atcgcaaact taaagatcgt caccggcagg ttggcgatcg
    15241 gctgcatcat gtccgtgctc cagaactggt tggagagcgc ggtaaacagc agcggcgcgg
    15301 tttcaccggc aatacgggca atcgccagca ggataccggt cataatcccg gacaccgacg
    15361 ctttcagcgt aatcgcggag atcatcttcc acttcggtgt acccagcgca taagccgctt
    15421 cacgcaggct gtccggcacc agtttcagca tgttctcggt ggtgcggata acgatcggca
    15481 cctgcaacag cgccagcgca attacgcccg cccagccgga gaagtgctcc atctgtgcca
    15541 ccacgatggt gtagacaaac agaccaacca caatcgacgg cgcggagagc agaatgtcgt
    15601 taatgaagcg aatcacttct gccagccagg atttacgacc atattccgcc agataaatcc
    15661 ccgccataat gcccagcggc gtaccgaata ccgtggccca caaaattaac agcccgctac
    15721 ccgccagggc gttcgccaga ccaccacctt ccgtattggg cggcggcgtc atttcagtga
    15781 acagcgccag cgacataccg tcgataccgc gagtgatggt ggacattaaa atccagatca
    15841 gccagaacag gccgaaggcc atcgtcgcca tcgagagcgt cagcgcaata cggtttttga
    15901 ggcggcgacg cgcctgcatt ttgcggcgag attcagccag cgccgcagtg gtttgcattt
    15961 caaccatagc cattagcgtg ccccctcatt cttagccagg cgcataatca taaacttcga
    16021 tgcggcgagg acgatgaagg taatcacaaa caggatcagg cccagttcca tcagtgcggc
    16081 aacgtgcaga ccggattccg cttccgcaaa ttcgttcgcc agcgcagagg tgatactatt
    16141 gcccggcatg tacagcgagg cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac
    16201 cgccatggtt tcaccgagcg cgcggcccag ccccagcatg atgccgccga taacaccatt
    16261 tttggtgaac ggaagaacga tacgccagat aacttcccag gtggtgcagc caataccgta
    16321 ggccgactct ttcatcatca ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat
    16381 gtacggaata atcatgatgg cgaggatcac gcctgccgcg aggataccga taccaaatgc
    16441 ggggccagag aacagcgcgc caacaatcgg gatattcgac atgatattgc cgaccggctc
    16501 ctgaaagtaa acggcgaaca gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat
    16561 acttggaatg gctgccagca gctcaatggc gatacccagc gggcgtttca gccagccagg
    16621 cgcaagctca gtcaggaaca gggcgatacc gaaactcacc gggacggcga tcagcagcgc
    16681 gataaacgaa gtcaccaacg taccgtagat cggcaccagc gccccgtaga tatcgttcgg
    16741 tgcatcccac tctttggtcc ataggaaagc cagaccaaat ttctgaatgc tcggccagga
    16801 ggagatgatc agagagacaa taatgccacc caacatcaat agcacaatca gcgccgccag
    16861 ttttaccagc acgctgaaaa ttatgtcgcc ctttttaccc ggtgggttaa aagcgggctt
    16921 ggttgcagcc ataagttact cttcagttaa acgcgtttac aaagtcattt tgtaatgccg
    16981 gatgcggcgt aaaacaccgt acccggcctg gagttttatt agtacagcgg cttaccgcta
    17041 ctgtctttaa tattggtctt ccacgcagcg cgaacctgtt caactacact atccggcagg
    17101 ctagcgtaat ccaggtcgtt cgcctgttta gccccggttt tgtacgccca gtcgaagaat
    17161 ttcagcacct ctgtgccttg ttctggtttc ttctgatctt tgtggatcag aatgaacgtg
    17221 gtagaggtaa taggccatgc atcttcgcct ttctggttgg tcagatcctg agcgaaggtt
    17281 ttgctccagt ctgcaccttt tgctgcatta gcgaagtttt cttcggtcgg actaaccggt
    17341 ttaccatcag cggagatcag tttggtgtac gccaggttgt tctgcttcgc gtaagcatat
    17401 tcaacataac caattgcacc cggcagacgc tgaacgaacg cggcgatacc gtcgttacct
    17461 ttaccgccca gaccgatcgg ccattttacg gtagagccag taccaacgtt gtttttccac
    17521 tcttcgttca ctttcgccag gtagctggtg aagacgaagg aagtcccgga gccatctgcg
    17581 cggcgtacta cagcaatgtt ttgtgaaggc agtttcagac ccggattcag tttggcgatg
    17641 gcttcatcat cccacttctt gattttgcct aggtagatgt cgccgagggt tttaccatcc
    17701 agcaccagtt cgccagactt cagccctgga atgttaaccg ccagcaccac gccgccaatc
    17761 acggtcggga actggaacag accttcctga gccagttttt cgtcagacag cggcgcgtca
    17821 gaggcaccaa aatcaacggt attagcgata atctgtttta cgccaccgga agaaccgata
    17881 ccctggtagt taactttatt accggtttct ttctggtaag tatcagccca tttggcatac
    17941 accggcgcag ggaaggttgc acctgcacct gtcaggcttg cttctgcaaa cacagagaaa
    18001 gcactcatcg ataaggtcgc ggcgacaaca gttgcgacgg tggtacgcat aactttcata
    18061 atgtctcctg ggaggagtca taaagcattg tttgttggct acgagaagca aaataggaca
    18121 aacaggtgac agttatatat aaggaatatg acatttttat gacagaaagg aattaccaaa
    18181 taacttactg attttaaatg tgaagatgaa aatcccacat gccatctttt tgtcatgatg
    18241 catttaaaag tgataaagaa atgggtgaga tgacaacgat gtgatgaaca atcgttgtca
    18301 gacctattag cgcacaatct caacagttat cgtcgctgtt gcagtaaatt gccccggggc
    18361 tgggcgagct ccagtaaggc tcgcaggagc agctgaaaaa gttacactac ctgatttttc
    18421 accatacact gttgatttat ccatgtctgt tggcaactca ccaccgttca ctttgatttc
    18481 cttattattt ttgtcgtaaa tttttatccc cacgttagag tttgaagtta cgaccattga
    18541 attgtccgta ttgggttcgg tagcataaaa agacgtactg atatcatcgg tattcagtat
    18601 ccctaggttt tcacactcaa tagtgactgt tttagtaatt ttatgtgttg ttaccgcact
    18661 tccaacaaca gatgaaaaat ctgcagctgg tatttcttta aaatcaaaac aaactggctt
    18721 taaattattt atttcgcaac tttgcggagc tgtaatatcg ccccgaatat aaacatcagc
    18781 catcggctgt gagtaatcca ccggggtgtc acggctaatt gttccataca atgtcactat
    18841 ctttgtttca ggaataatca attttcctgc aaatgtcttc ttgatataaa aatatactat
    18901 cgctttagcc ccactggata tacttaaagg ttcaatgcgg ttgcaattgt actttgtatt
    18961 accagacggg ttgttagcat gatattcaaa aggtgcattc acatacccaa ccccaagaat
    19021 atctatagca atcccaacat caaggttcgg attaagatca taataattaa tattagagtg
    19081 aaccccagca taagttaatg cctggtttat ttttgacgta taatagatat aggggaattc
    19141 tcctacgtta ccagtatcac aatgacagcg tccaagatat gaggatccat tagttacagt
    19201 ggtgttaaat gtcgttccaa ccttattttc gttcgcggta ataagatatc ccgagaaatc
    19261 aagcgtactg tgaaatacgc cattatcagg ggtacagata cctttaggag ctgcaagtgc
    19321 tacactactt aacgagagca taaacacaga aaagcaccac tgtttgatat acttgttcat
    19381 aaaaatcctt ttaatgacat gtcacaccct gttcaatt
//
LOCUS       DADLXX010000075        19376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLXX010000075 DADLXX010000000
VERSION     DADLXX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.075"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNX27_002510"
     CDS             complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNX27_002510"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7883687.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNX27_002511"
     CDS             complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNX27_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7883688.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGPLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNX27_002512"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNX27_002512"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7883689.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNX27_002513"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNX27_002513"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7883690.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNX27_002514"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNX27_002514"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7883691.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNX27_002515"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNX27_002515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7883692.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNX27_002516"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNX27_002516"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7883693.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNX27_002517"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNX27_002517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7883694.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNX27_002518"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNX27_002518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7883695.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNX27_002519"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNX27_002519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7883696.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNX27_002520"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNX27_002520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7883697.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNX27_002521"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNX27_002521"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7883698.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNX27_002522"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNX27_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7883699.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNX27_002523"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNX27_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7883700.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNX27_002524"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNX27_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7883701.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="JNX27_002525"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="JNX27_002525"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7883702.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNX27_002526"
                     /pseudo
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNX27_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNX27_002527"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNX27_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7883703.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLKASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4628 a         5119 c         4860 g         4769 t
ORIGIN      
        1 cggcagccct tttttatcta tgatagtgat tactcaaccg taaccgattt tgccaggtta
       61 cgcggctggt caacgtcggt gcctttgatc agcgcgacat ggtaagccag cagctgcagc
      121 ggaacggtgt agaagatcgg tgcaatcacc tcttccacat gcggcatctc gatgatgtgc
      181 atgttatcgc tacttacaaa acccgcatcc tgatcggcga agacatacaa ttgaccgcca
      241 cgcgcgcgaa cttcttcaat gttggatttc agtttttcca acagttcgtt gttcggtgca
      301 acgacgataa ccggcatatc cgcgtcaatc agcgccagcg ggccgtgttt cagctcgcct
      361 gcagcgtagg cttcagcgtg aatgtaagag atctctttca gcttcaatgc gccttccagc
      421 gcgattgggt actgatcgcc acggcccagg aacagcgcgt gatgtttgtc agagaaatct
      481 tctgccagag cttcaatgcg tttatcctga gacagcatct gctcaatacg gctcggcagc
      541 gcctgcagac catgcacaat gtcatgttca atggaggcat ccagaccttt caggcgagac
      601 agcttcgcca ccagcatcaa cagcacagtt aactgagtgg tgaatgcttt agtggacgcc
      661 acgccgattt ccgtacccgc gttggtcatc agcgccagat cggattcgcg caccagagaa
      721 gaacccggaa cgttacagat tgccagcgaa ccaaggtaac ccagctcttt cgacagacgc
      781 aggcccgcca gggtatccgc ggtttcgcca gactgtgaca aggtgatcat caggctgtta
      841 cgacgcacgg cagatttacg atagcggaat tcagaggcga tttcgacgtc gcacggaata
      901 cctgccagcg attcaaacca gtagcgggaa accataccgg agttataaga agtaccacag
      961 gcgaggatct gaatatgctc aaccttcgac agcagttcgt cggcgttcgg tcccagctcg
     1021 cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg tgttttttat cgcgttcggc
     1081 tgttcgtaga tctctttctg catgtagtga cggtaaatac ctttatcgcc cgcgtcatat
     1141 tgcagattgg attcgatatc ctgacgtttt acttccgcgc cagttttatc gaagatgttt
     1201 accgaacggc gagtgatttc cgcaatatcg ccctcttcaa ggaagataaa gcgacgggtc
     1261 accggcaaca gcgccagctg gtcagaagcg ataaagtttt cgcccatacc caggccaatc
     1321 accagcggac taccagaacg tgccgccagc agggtatccg ggtgacggga gtccatgatc
     1381 actgtaccgt acgcaccgcg cagctgcggg atagcacgca gaacagcctc acgcagagtc
     1441 ccgccttgtt tcagctccca gttcaccaga tgggcaatca cttcggtgtc ggtttcagaa
     1501 acgaaggtat agccacgcgc ttttaactct tcacgcagcg gttcatggtt ttcgatgatg
     1561 ccattatgca ccaccacaat gtgttcagaa acatgcggat gcgcgttagc ttctgaaggt
     1621 tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc cgccatgcag aggatgttct
     1681 tccgctgcct gagccagcat ctggacttta ccgaggcgac gcaggcgagt catatgacct
     1741 tccgcatcaa caacggccag accggcagag tcatatccgc ggtattccag acgacgtaaa
     1801 ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg cgccaacaat tccacacata
     1861 gtttttgatt ccgatttata tcgttgtcgg tcaacctgta tgcccgtttt ttcgggcgcc
     1921 ccgagccttg tagagagtgg ggttattttt atagttactg cttgtgggag ggagattatg
     1981 ttatctcctc atcccagatg accggattac ccggccagaa ttactttttc tttaccggac
     2041 gacgccagcc ttctttctga gtctgcggca cacggctgat agctaatgca ttttcgccga
     2101 cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc tttgcctact gttaccgggg
     2161 ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc gataatggtc ttaaatttat
     2221 tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat gttaacgtta tcgccaattt
     2281 ccgcatcgcc taggtaagtc agatgcccgg ctttcgtgcc tttacccaga cgcgcttttt
     2341 tcatctcaac gaagttacca acgtgtgcac cttccagcaa ctcagcacca ggacgcagac
     2401 gggcaaacgg gccaatagta caggccgctg ccagattcgc gtcttccaca acggtatacg
     2461 gactgatttc gcaatcatcg ccaatcacgc tgtttttaat cacgcaaccg gtgccgattt
     2521 tcacgcgatg accgagagtc acgttgccct cgatgataac gttagtatca atttcaacat
     2581 cgcgcccgtg agtaagcggg ccgcgcagat caaaacgcgc cggatcgcgc agcataacgc
     2641 ctgctaacag cagtttttca gcctgttcgg actggtaaac gcgctccaga cgggagagtt
     2701 gcaggcggtt attcacacct tctacttcgc ttaaacgttg cggatgaacg gcgacgattt
     2761 cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat gtagtattcg ccctgagcat
     2821 tattgttggt cagcttcgcc agccagcgtt tcatatctgc gccgttggca atcagaatgc
     2881 cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc atctttgtgc tcaacaatgc
     2941 cggtaacttt gccattttca cgggtgatac gtccataacc ggtcggatca tccagtttca
     3001 ccgtcagcag accaatgcca ccctggggtt tagcatcacg caggcgctgg agtgtttcga
     3061 cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc ttcatcatcg gcaaagaaag
     3121 gtgcggcctg ctgcattgca tgacccgtac ccagctgctc tgcctgaagc acccagttaa
     3181 ggttgtcgtc tttcaacgcc tgtttaagca gatcgccgcc gtgaccgtac accaggtgaa
     3241 cgtgcgctgc gcctaattca ttcgcagcat caatgacatg ctgaaccatc gctttcccgg
     3301 caagggtatg cagcactttc ggaagatcgg aatacatgcg cgtgcctttg cctgcggcaa
     3361 ggatcactac gctcatagca ttattcaaca tacgcgtcct gactgtaatt tgagaacgaa
     3421 tttaaaccgc ttcaccttga aaaaactaca tttttttcat cgtgaaatgg acagaggata
     3481 aattgttcaa taacggatta tccccgctga cgaaagtgcc attttcgacc atcgtcacgc
     3541 cttttgtctg cagaaaatta aggcagtacc ttcatttaag cgtcaggtgg atgtttttgc
     3601 tcttatttcg atcaatgaat aaacgggaaa acaggggtgt tttatatcag caggatctat
     3661 gtgaacgctc ttcaggacgg gtcaacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacttg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagggcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggagtttat tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgttg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcaag tcgataggca gtaaatccat cagattcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caagaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaagccac ccggcctttc gctggtgtat
    10441 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct acccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatca
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtct
    12961 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgca gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacgctaaa gtcgatggtt
    13201 cgagcatcaa tacgcggcgg agtaccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagagagcgg aatggatggc ggatcgccag cacggccacc gctgtaatta
    13321 tccagaccga tatgaatttt gccgtcgagg aacgtcccaa cggtgagcac cacagctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagcgcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cagccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactgccc tgtggataac aaggatccga cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aacggttgtt ctttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctgccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata accagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatggta cgcatgattg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat aacttttata aagatgctgt
    14881 agtggccggt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggtttt ttattgaatg attattgcat gtgtgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttaac gaagagtttg gcctggcacc gttcctgccg gatcagattc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt cgggattggc ggcaagttga
    15961 gtgatggtca tcgtcacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggcca tgcgggcctg aacggcgata ttctggtgtg gaacccggta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gagtatggcc agttgctgtt cgcgagagcg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gctacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatataa cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaagcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga gcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctacgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaata aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtctagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag tcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgctta cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgtac aacaagatgt tgggtgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt aacgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgcccaggc gggtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcaa ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc cttcaataag cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ttgcacttta tctaaccata gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagtaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacgatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gagtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg ggtaat
//
LOCUS       DADLXX010000076        18261 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLXX010000076 DADLXX010000000
VERSION     DADLXX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.076"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNX27_002528"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNX27_002528"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7883704.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNX27_002529"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNX27_002529"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY7883705.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..2943)
                     /locus_tag="JNX27_002530"
     CDS             complement(1729..2943)
                     /locus_tag="JNX27_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883706.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            complement(3183..4328)
                     /locus_tag="JNX27_002531"
     CDS             complement(3183..4328)
                     /locus_tag="JNX27_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883707.1"
                     /translation="MGVILMSLNMASLETLCQTNAAGVSIVISSLGIGRLSVLLFAGL
                     LSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFGFLAGMANSFLDAGTYPSLME
                     AFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIMFINALFLYR
                     CTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYLVSQWLAQYGQFV
                     AGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFISFIALFTVCLHP
                     TFYVVIIFAFVIGFTSAGGVVQIGLTLIAERFPYAKGKATGIYYSAGSIATFTIPLIT
                     AHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHSLKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="JNX27_002532"
                     /pseudo
     CDS             complement(4487..4843)
                     /locus_tag="JNX27_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="JNX27_002533"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JNX27_002533"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNX27_002534"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNX27_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7883708.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="JNX27_002535"
     CDS             6773..9829
                     /locus_tag="JNX27_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7883709.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="JNX27_002536"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="JNX27_002536"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7883710.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="JNX27_002537"
     CDS             10336..10524
                     /locus_tag="JNX27_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883711.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="JNX27_002538"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="JNX27_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7883712.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="JNX27_002539"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="JNX27_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7883713.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="JNX27_002540"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="JNX27_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7883714.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="JNX27_002541"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="JNX27_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7883715.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTVHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGFKQQVLARIAQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="JNX27_002542"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="JNX27_002542"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7883716.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="JNX27_002543"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="JNX27_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7883717.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="JNX27_002544"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="JNX27_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7883718.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /locus_tag="JNX27_002545"
     CDS             complement(17801..18037)
                     /locus_tag="JNX27_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7883719.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4804 a         4405 c         4690 g         4362 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcttgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgaacg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagctatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acattccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgaca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaataaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttactgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcagcg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg gacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgtccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gctagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggcctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gcatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac tttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagtt atcgcggcca ggaaacgcac gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gtccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gtttaaacag caggtgctgg cccgaatcgc tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg gttgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca gcgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcggcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt g
//
LOCUS       DADLXX010000077        18180 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLXX010000077 DADLXX010000000
VERSION     DADLXX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.077"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..454
                     /locus_tag="JNX27_002546"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNX27_002546"
                     /inference="COORDINATES: protein motif:HMM:NF013106.1"
                     /note="penicillin-insensitive
                     transglycosylase/transpeptidase; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            941..2090
                     /locus_tag="JNX27_002547"
                     /pseudo
     CDS             941..2090
                     /locus_tag="JNX27_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            2240..2671
                     /gene="ndk"
                     /locus_tag="JNX27_002548"
     CDS             2240..2671
                     /gene="ndk"
                     /locus_tag="JNX27_002548"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7883720.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2821..3975
                     /gene="trmG/rlmN"
                     /locus_tag="JNX27_002549"
     CDS             2821..3975
                     /gene="trmG/rlmN"
                     /locus_tag="JNX27_002549"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7883721.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4260..5273
                     /gene="rodZ"
                     /locus_tag="JNX27_002550"
     CDS             4260..5273
                     /gene="rodZ"
                     /locus_tag="JNX27_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7883722.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5300..6418
                     /gene="ispG"
                     /locus_tag="JNX27_002551"
                     /gene_synonym="gcpE"
     CDS             5300..6418
                     /gene="ispG"
                     /locus_tag="JNX27_002551"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7883723.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6529..7803
                     /gene="hisS"
                     /locus_tag="JNX27_002552"
     CDS             6529..7803
                     /gene="hisS"
                     /locus_tag="JNX27_002552"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7883724.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7821..8441
                     /locus_tag="JNX27_002553"
     CDS             7821..8441
                     /locus_tag="JNX27_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7883725.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8452..9630
                     /gene="bamB"
                     /locus_tag="JNX27_002554"
     CDS             8452..9630
                     /gene="bamB"
                     /locus_tag="JNX27_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7883726.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9748..11220
                     /gene="der"
                     /locus_tag="JNX27_002555"
     CDS             9748..11220
                     /gene="der"
                     /locus_tag="JNX27_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7883727.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11290..11505
                     /locus_tag="JNX27_002556"
     CDS             11290..11505
                     /locus_tag="JNX27_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883728.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11502..12872)
                     /gene="xseA"
                     /locus_tag="JNX27_002557"
     CDS             complement(11502..12872)
                     /gene="xseA"
                     /locus_tag="JNX27_002557"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7883729.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13034..14500
                     /gene="guaB"
                     /locus_tag="JNX27_002558"
     CDS             13034..14500
                     /gene="guaB"
                     /locus_tag="JNX27_002558"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7883730.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14569..16146
                     /gene="guaA"
                     /locus_tag="JNX27_002559"
     CDS             14569..16146
                     /gene="guaA"
                     /locus_tag="JNX27_002559"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7883731.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16241..16780)
                     /locus_tag="JNX27_002560"
     CDS             complement(16241..16780)
                     /locus_tag="JNX27_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883732.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16796..17314)
                     /locus_tag="JNX27_002561"
     CDS             complement(16796..17314)
                     /locus_tag="JNX27_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7883733.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17625..17816)
                     /locus_tag="JNX27_002562"
     CDS             complement(17625..17816)
                     /locus_tag="JNX27_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY7883734.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17834..17986
                     /locus_tag="JNX27_002563"
     CDS             17834..17986
                     /locus_tag="JNX27_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883735.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4300 a         4453 c         4994 g         4433 t
ORIGIN      
        1 ttaccttgaa gcgctgatca attatgaaga tcgctggttc tggaagcatc cgggggttaa
       61 tccattctcg gtggcgcgcg cagcatggca agatctcact tcgggacggg ttatttccgg
      121 tggcagcacg ctcactatgc aggttgctcg tctgcttgat cctcacccca aaacatttgg
      181 tggcaaaatt ctccagctct ggcgcgcgtt gcaactggag tggcatttgt ctaagcgtga
      241 aattctgatc ttgtatctta accgcgctcc gtttggcggt acgttgcagg ggatcggtac
      301 ggcaagttgg gcttatctcg gaaaatcgcc tgcgaattta agctattccg aggcggcaat
      361 gctggcggtg ttaccgcaag tgctcagccg actgcgcccg gatcgttggc cggagcgtgc
      421 cgaagccgcg cgtaataaag tgctcgaacg gatggctgcg caaggtgtgt ggtcccgtga
      481 gcaggtaaaa gagtcaaggg aagaacccat ctggctggca ccccgacaaa tgccgcaact
      541 ggcaccgctg ttttcgcgca tgatgctcgg taaaagcaaa agcgacaaaa tcgttactac
      601 gctggatgcc ggtcttcaac gacgtctgga agaactggcg caaaactgga aagggcggtt
      661 gccaccgcgc agctcactgg cgatgatcgt ggttaatcat accgatatgc gtgttcgcgg
      721 ctgggtggga tcggttgatc tcaacgatga ttcacgcttt ggtcatgttg atatggtcaa
      781 tgcgatccga tcgccaggat cggtgctcaa accgtttgtt tatggtctgg cgctggatga
      841 aggcttgatc cacccggcat cactgctgca agacgtcccc cggcgcaccg gtgattatcg
      901 accaggtaac tttgatagcg gttttcatgg cccgatcaac atgagcgagg cgctggtgcg
      961 ctcgctgaac ttaccagctg tgcaggtgct ggaagcctat ggaccgaaac ggtttgcggc
     1021 aaagttacgc aatgttggat tgccgttata tttgcccaac ggcgctgcgc cgaatctttc
     1081 actcattctc ggcggcgctg gtgcaaaact ggaagatatg gcggcagcgt ataccgcgtt
     1141 tgctcgccac ggcaaggcag gcaaattgcg cttacagcct gacgatccgc tgcttgaacg
     1201 gcctttaatg tcgtcggggg cggcgtggat cattcgccgg attatggctg atgaagcgca
     1261 acccttgccg gatagtgcct tgccgcgcgt cgccccactg gcatggaaaa cgggcaccag
     1321 ctatggctat cgtgacgcct gggcgattgg ggttaacgct cgctatgtca ttgggatctg
     1381 gactggcaga cctgacggca cgcccgttgt tggtcagttt ggctttgcca gtgccgtacc
     1441 attagttgaa tcaggtcaat aatatcttac tgtcgcgcag tgcgaatctg ccggaagacc
     1501 cgcgcccgaa ctcagtcact cgtggcgtta tctgctggcc gggcggacag tctttgccgg
     1561 aaggtgacgg taactgtcgc cgccgcctgg caacctggct gctggatggc agccagccgc
     1621 caacgctgtt actgtcagag caggaaggga ttaatggcat tcgcttcccc atctggctgg
     1681 atgacaaagg gcagcgcgtc gccgctgact tcccgcaggc gaagcaagaa atggtaaatg
     1741 tctggccgct accgctggaa ccctggctgc ccgcatcaga acgtcgcgct gtgcgcttac
     1801 caccggcctc gacaatttgc ccgccatacg gtcacgatgc ccagctccct ctgcagctga
     1861 caggcgttcg cgatggcgcg attattaaac gtttaccggg cgcagcggaa gcgactttgc
     1921 cgttgcaatc aagtggaggg gcaggtgaac gctggtggtt tctgaatggc gaaccgttaa
     1981 ctgaacgcgg gcgcaacgtg accctgcatt tgacggataa aggtgattat caattgctgg
     2041 tgatggatga tgtggggcaa atcgcgacag tgaaatttgt catgcaatag tctgttttgt
     2101 ccctgattgt tgctaaaact cattttattt taaaaaaaat gttaccttca tcaatagtca
     2161 acggccctgt tgctcattat aatccgcgcc atctcgtacg ctggtacaga caacaacaga
     2221 acaatttaca gaggtaaaaa tggctattga acgtactttt tccatcatca aaccgaacgc
     2281 ggtagcaaaa aacgtcattg gtaatatctt tgcgcgcttt gaagctgcag ggttcaaaat
     2341 tgtcggcacc aaaatgctgc acctgaccgt tgaacaggca cgtggctttt atgctgaaca
     2401 cgatggaaaa ccgttctttg atggtctggt tgaattcatg acctctggcc cgatcgtggt
     2461 ttccgtgctg gaaggtgaaa acgccgttca gcgtcaccgc gatctgctgg gcgcgaccaa
     2521 tccggcaaac gcactggctg gtactctgcg cgctgattac gctgacagcc tgaccgaaaa
     2581 cggtacccac ggttctgatt ccgtcgaatc tgccgctcgc gaaatcgctt atttcttcgg
     2641 cgaaggcgaa gtgtgcccgc gcacccgtta ataatttcgt aaatgccgcg tgcaaacgtg
     2701 gcatccgtgc gccagaattt gtacaatgta gcgcccccgg acgagcagcc gctcaccggg
     2761 gcgtttcttt tttcaaccct ccaggggcca taacgtgtaa taacgaggcc ggaatagcat
     2821 atgtctgaac aattagtcac acctgaaaac gtcaccacga aagatggaaa aatcaacctg
     2881 ctggatctca accgtcagca gatgcgggag ttttttaaag atttaggtga aaaaaccttc
     2941 cgcgccgatc aggtgatgaa gtggatgtat cactattgct gcgacaactt tgatgagatg
     3001 accgacatca acaaagtgtt gcgcggcaaa ctgaaagagg tggcggaaat ccgcgcaccg
     3061 gaagtggttg aagaacagcg ttcatccgac ggcaccatta aatgggcgat cgccgttggc
     3121 gatcagcgcg tcgaaacggt gtatatcccg gaagacgacc gtgccacgct ctgcgtctct
     3181 tcgcaggtgg ggtgtgcgct ggagtgtaaa ttctgttcca ccgcccagca gggctttaac
     3241 cgcaacctgc gggtatcgga aattattggc caggtgtggc gtgcggcgaa aatcgtcggc
     3301 gcagcaaaag tcaccggtca gcgcccaatc actaacgtcg tgatgatggg catgggcgag
     3361 ccgctgctca acctgaacaa cgtcgttccg gcgatggaaa tcatgcttga tgatttcggt
     3421 tttggcctgt ctaaacgtcg cgtgacgctt tccacttccg gtgtagttcc ggcgctggat
     3481 aaactgggcg atatgatcga cgttgcactg gcaatttccc tgcacgcgcc gaacgacgaa
     3541 attcgtgacg aaattgtgcc gatcaacaaa aagtacaata tcgaaacgtt ccttgctgcg
     3601 gtgcgccgtt atctggagaa atccaacgcc aatcagggcc gagtcactat tgaatacgtg
     3661 atgcttgacc acgttaacga cggcactgaa cacgcgcacc aactggcgga actgctgaaa
     3721 gatacgccgt gtaagatcaa cctgatcccg tggaacccgt tcccgggcgc gccgtatgga
     3781 cgcagttcga acagccgtat cgaccgcttc tcaaaagtgc tgatgagcta cggtttcacc
     3841 actattgtgc gtaaaacgcg tggtgatgat atcgatgctg cctgtggtca gttggcgggc
     3901 gatgttatcg accgtacgaa acgtactctg cgtaaacgga tgcagggtga agccatcgat
     3961 attaaagcgg tctgatagcc attacgccac ggttacttta tgtaccgtgg cgtaatcttt
     4021 tatgaatgaa gtattaagtt gcaattgtct ggtcaatatt aacggtgcgt ttttgctgac
     4081 tttaaggcag tatgtaacga tgcaacagta agttagcctc agtgaatgtg ggctttgtca
     4141 cgagcacaca gacggtctta tactgtatga taacggttaa cttaacggat gtttcgcggt
     4201 gtgggtgagc atgatggttc accggcatct caattctcat ttaaacgtac ctgcagcgaa
     4261 tgaatactga agccacgcac gaccaaaatg aagcacttac taccggcgct cgcctgcgta
     4321 atgctcgcga acaactagga cttagtcagc aggccgttgc cgagcgactt tgcctgaagg
     4381 tttccacggt acgcgacatt gaagaagata aggcacccgc cgatcttgct tcaacattcc
     4441 tgcgcggata tatccgctct tatgcgcgtc tggtacatat tccagaagaa gaactgctgc
     4501 cagggctgga aaagcaggct ccacttcggg ctgcaaaagt tgcgccaatg cagagttttt
     4561 ccctcggtaa acgccgcaaa aaacgcgacg gctggctgat gaccttcacc tggctggtgt
     4621 tgtttgtggt tatcggcctg agcggtgcct ggtggtggca agaccacaaa gctcagcagg
     4681 aagagatcac cactatggcc gatcaatctt cggcggaact gagcagtaat agcgagcagg
     4741 ggcagagtgt tccgttaaat acgtcgacaa ctacagaccc ggctacaacg tccacgccgc
     4801 cagcgtctgt ggatactacc gcaaccaaca cgcaaacacc tgccgtaact gcgccagcac
     4861 cagctgttga tccgcaacag aatgcggttg tttcgccttc gcaggcaaat gttgataccg
     4921 ccgcgacccc ggtaccaacg gcagcaacaa cgccagatgg tgctgcgccg ttgccaaccg
     4981 atcaggctgg cgtgaccacg ccggcggctg atccgaatgc gctggtgatg aactttactg
     5041 ccgattgctg gctggaggtc actgatgcta ccggtaaaaa attgtttagc gggatgcagc
     5101 gtaaagacgg taatttgaat ttaaccggcc aggcaccgta taaactgaaa attggtgcgc
     5161 cagccgcagt acagatccag tatcaaggga aacctgtcga tctgagtcgt tttatcagaa
     5221 ctaaccaggt tgcgcgtctg accctcaatg ccgaacaatc accggcgcag taacagacgg
     5281 gtaacgcggg agatttttca tgcataacca ggctccaatt caacgtagaa aatcaacacg
     5341 tatttacgtt gggaatgtgc cgattggcga tggtgctccc atcgccgtac agtccatgac
     5401 caatacgcgt acgacagatg tcgaagcaac ggtcaatcaa atcaaggcgc tggaacgcgt
     5461 tggcgctgat atcgtccgtg tctccgtacc gacgatggac gcggcagaag cgttcaaact
     5521 catcaaacag cgggttaacg tgccgctggt ggctgacatc cacttcgact atcgcattgc
     5581 gctgaaagta gcggaatacg gcgtcgattg tctgcgtatt aaccctggta atatcggtaa
     5641 tgaagagcgt attcgcatgg tggttgactg tgcgcgcgat aaaaacattc cgatccgtat
     5701 tggcgttaac gccggatcgc tggaaaaaga tctgcaagaa aagtatggcg aaccgacgcc
     5761 gcaggcgttg ctggaatccg ccatgcgtca tgttgatcat ctcgatcgcc tgaacttcga
     5821 tcagttcaaa gtcagcgtga aagcgtctga cgtcttcctc gctgttgagt cttatcgttt
     5881 gctggcaaaa cagatcgatc agccgctgca tctggggatc accgaagccg gtggtgcacg
     5941 cagcggggca gtaaaatccg ccattggttt aggtctgctg ctgtcggaag gcatcggcga
     6001 cacgctgcgc gtatcgctgg cggccgatcc ggtcgaagag atcaaagtcg gtttcgatat
     6061 tttgaaatcg ctgcgtatcc gttcgcgagg gattaacttc atcgcctgcc cgacctgttc
     6121 gcgtcaggaa tttgatgtta tcggtacggt taacgcgctg gagcaacgcc tggaagatat
     6181 catcactccg atggacgttt cgattatcgg ctgcgtggtg aatggcccag gtgaggcgct
     6241 ggtttctaca ctcggcgtca ccggcggcaa caagaaaagc ggcctctatg aagatggcgt
     6301 gcgcaaagac cgtctggaca acaacgatat gatcgaccag ctggaagcac gcattcgtgc
     6361 gaaagccagt cagctggacg aagcgcgtcg aattgacgtt cagcaggttg aaaaataata
     6421 acgtgatggg aagcgcctcg cttcccgtgt atgattgaac ccgcatggct cccgaaacat
     6481 tgagggaagc gttgagggtt catttttata ttcagaaaga gaataaacgt ggcaaaaaac
     6541 attcaagcca ttcgcggcat gaacgattac ctgcctggcg aaacggccat ctggcagcgc
     6601 attgaaggca cactgaaaaa cgtgctcggc agctacggtt acagtgaaat ccgcttgccg
     6661 attgtagagc agaccccgct attcaaacgt gcgattggtg aagtcaccga cgtggttgaa
     6721 aaagagatgt acacctttga ggatcgcaat ggcgacagcc tgactctgcg ccctgaaggg
     6781 acggcgggct gtgtacgcgc cggcatcgag catggtcttc tgtacaatca ggaacagcgt
     6841 ctgtggtata tcgggccgat gttccgtcac gagcgtccgc agaaagggcg ttatcgtcag
     6901 ttccatcagt tgggctgcga agttttcggt ctgcaaggtc cggatatcga cgctgaactg
     6961 attatgctca ctgcccgctg gtggcgtgcg ctgggtatct tcgaacacgt aactcttgag
     7021 ctgaattcta tcggttcgct ggaagcacgc gccaattacc gcgatgcgct ggtggcattc
     7081 cttgagcagc ataaagaaaa gctggacgaa gactgcaaac gccgcatgta cactaacccg
     7141 ctgcgcgtgc tggattcaaa aaatccggaa gtgcaggcgc ttctcaacga cgctccggca
     7201 ttaggtgatt atctggacga ggaatctcgt gagcattttg ccggtctgtg caaactgctg
     7261 gagagcgcgg ggatcgctta caccgtaaac cagcgtctgg tgcgtggtct ggattactat
     7321 aaccgtaccg ttttcgagtg ggtgactaac agtctcggct cccagggcac cgtgtgtgca
     7381 ggcggtcgtt atgacggtct tgtggaacaa ctgggcggtc gtgcaacacc ggctgtcggt
     7441 tttgcgatgg gcctcgaacg tcttgtattg ttagtacagg ccgttaatcc ggaatttaaa
     7501 gccgatcctg ttgtcgatat atacctggtg gcttcaggtg ctgatacaca atctgcggct
     7561 atggcattag ctgagcgtct gcgtgatgaa ttactgggcg tgaaattgat gaccaaccac
     7621 ggcggcggca actttaagaa acagtttgcc cgtgctgata aatggggtgc ccgcgttgct
     7681 gtggtgctgg gtgagtctga agtggctaac ggcacagcag tagtgaagga tttgcgctct
     7741 ggtgagcaaa cggcagttgc gcaggatagc gtagccgcgc atttgcgcac gttactgggt
     7801 taaggaagga gaaggacagc gtggaaattt acgagaacga aaacgaccag gtagaagcgg
     7861 ttaaacgctt ttttgctgaa aatggcaaag cactggctgt tggggtgatt ttgggcgttg
     7921 gcgcactgat tggctggcgc tactggaaca gccatcaggt tgattctgca cgctccgctt
     7981 ctcttgccta tcaaaatgcg gttaccgcag tgagcgaagg caaaccggat agcatcccgg
     8041 cggcggaaaa atttgctgct gaaaataaaa atacttatgg tgcgctggct tctttggaac
     8101 ttgcgcagca atttgttgac aaaaatgaac tagagaaagc tgccgcccag ttacaacagg
     8161 ggctggcaga cacgagcgat gaaaatctca aagccgtgat aaatctgcgt cttgctcgcg
     8221 ttcaggtaca gctcaagcag gctgatgccg cgctgaaaac ccttgatacc atcaaaggtg
     8281 aagggtgggc tgccattgtt gccgacctgc gtggtgaagc attgctgagc aaaggtgata
     8341 agcaaggtgc gcgtagtgca tgggaagcag gcgtgaaaag cgatgttact ccggcactga
     8401 gcgaaatgat gcagatgaaa attaataatt tgtccatctg agagggaccc gatgcaattg
     8461 cgtaaattac tgctgccagg actgctttcc gttacccttt taagcggctg ttcgctgttt
     8521 aacagcgaag aagatgtggt aaagatgtcc ccattgccaa ccgttgaaaa ccagtttacg
     8581 ccgaccacgg cgtggagcac ttccgttggt agcggcattg gcaacttcta ttccaatctt
     8641 catccggcac tggcggacaa cgttgtctat gcagcggacc gcgctggttt ggtaaaagcg
     8701 ctgaatgcgg atgatggcaa agaaatctgg tctgtcagcc tggccgagaa agatggctgg
     8761 ttctctaaag agcctgcatt actttctggc ggtgtgaccg tgtctggtgg gcatgtctac
     8821 attggcagcg aaaaggcgca ggtttacgcg ctgaatacca gcgatggtac tgtggcatgg
     8881 caaactacag tcgcgggtga agcactttcg cgcccggtgg tcagcgacgg tctggtgtta
     8941 atccacacca gtaacggtca gttacaagcg ctgaacgaag ctgacggcgc tgtcaaatgg
     9001 acagttaacc tcgatatgcc ttcgctctct ttgcgtggcg agtctgcgcc ggcaacggct
     9061 tttggtgcgg ccgtcgtggg tggcgataat ggtcgcgtca gcgcagtgct gatggaacag
     9121 ggccagatga tttggcagca gcgtatttcc caggcgaccg gttctaccga aattgaccgt
     9181 ctgagcgatg ttgacacgac tcccgtcgtt gttaacggcg ttgttttcgc gctggcctat
     9241 aatggtaacc tgacggcgct tgatctgcgc agtggtcaga ttatgtggaa acgcgaactg
     9301 ggttcggtga atgatttcat cgtcgatggc aatcgcatct atctggtcga tcaaaatgac
     9361 cgggtgatgg cgttgaccat tgatggcggc gttacgctgt gggcacaaag cgatctgctg
     9421 catcgcctgc tgacttctcc ggtgctgtat aatggcaacc tggtggtcgg tgacagtgaa
     9481 ggttatctgc actggattaa cgtcgaagat ggtcgtttcg ttgcccagca aaaagttgat
     9541 agttccggtt tccagactga accggttgcc gctgacggca aactgctgat ccaggcaaaa
     9601 gacggaaccg tgtactctat tacacgttaa tcgtctctgt cgttcacttt gaaaacggct
     9661 cctggacagg ggccgttttc ctgtttttaa caacgacgcg aatatagcgt gcgttgtctg
     9721 atgatttata aaaatgaggc tttaaacatg gtacctgtgg tcgcgcttgt cgggcgccct
     9781 aacgtaggaa aatccacgtt atttaaccgt ctaactcgca cccgagatgc gctggttgcg
     9841 gatttcccgg gtctgactcg tgaccgtaag tacggtcgtg cggaaattga aggccgtgag
     9901 tttatctgta ttgataccgg cgggattgat ggcacagaag acggtgtaga aacccgcatg
     9961 gcggaacagt cgctgctggc gattgaagaa gcggacgtcg tactgtttat ggtggatgcg
    10021 cgcgcgggcc tgatgccggc agatgaagcg attgccaaac atctgcgctc ccgtgaaaaa
    10081 ccgaccttcc tggtggcaaa caaaactgac ggtctggatc ccgatcaggc agtggttgat
    10141 ttctactcgc ttggtttagg tgaaatctac ccgatcgccg cgtctcacgg tcgtggcgta
    10201 ttaagtctgc tggagcatgt gctgctgccg tggatggaag atctcgcacc gcaagaggaa
    10261 gtcgacgaag acgctgaata ctgggcgcaa tttgaagcgg aagagaacgg cgaagaagaa
    10321 gaggaagacg acttcgaccc gcaaagtctg ccgatcaaac tggcgattgt gggtcgtccg
    10381 aacgtaggta agtctacact cactaaccgt attcttggag aagagcgcgt tgttgtttac
    10441 gacatgcctg gcacgacgcg tgacagcatc tacatcccaa tggaacgcga tggacgtgag
    10501 tatgtgctca ttgacaccgc tggcgtacgt aaacgcggca aaatcaccga tgctgtagag
    10561 aaattctccg taatcaaaac gttgcaggcc attgaagacg ccaacgtggt gatgttagtg
    10621 attgatgcgc gcgaaggtat ttccgatcag gatctctcgc tgctgggctt tattctcaat
    10681 agtgggcgct cacttgtcat tgtggtgaat aagtgggatg gcctgagcca ggaagtgaaa
    10741 gagcaggtga aagaaacgct ggacttccgt ctgggcttta tcgattttgc tcgtgtgcac
    10801 tttatctctg ccttgcacgg cagtggtgtt ggtaacttgt ttgaatcagt acgtgaagcg
    10861 tatgacagct ccacccgtcg tgtggggacc tctatgctga cgcgcatcat gacgatggct
    10921 gttgaagatc accaaccgcc gctggtacgc ggtcgtcgtg tgaagctgaa atatgcccac
    10981 gctggtggtt ataacccgcc gattgtggtg attcacggta atcaggtgaa agacctgcct
    11041 gattcctaca agcgctactt gatgaactac ttccgcaaat cgctggacgt aatgggatcg
    11101 ccgattcgta ttcagttcaa agaaggggaa aacccgtatg cgaacaagcg taacaccctg
    11161 acgccaaccc agatgcgtaa acgtaagcgt ctgatgaagc acatcaagaa aaataaataa
    11221 ttatccgtaa gtattttatc tgccattccc tctatgaatg tagagggaat ggctaccgga
    11281 ggaattcata tggaactgca ttgtccaaaa tgtcagcatg tgcttgatca ggataacggc
    11341 catgcgcgtt gcccatcctg cggggaattt attgaaatga aagctctttg tccagactgc
    11401 catcaaccgt tgcaggtgtt aaaagcctgc ggtgcagtag attacttctg tcagcacggg
    11461 catggcttga tatcgaaaaa acgcgttgaa ttcgtgctgg cttaatgaac ctttttacgc
    11521 gattttttta ccggctggat gttttttact tcactttcta tccagccgtc ttccagacgt
    11581 gtggttagca tttcacccgc tttaacttgc ttcacttttt tcagtacatt gccgtcagta
    11641 gcagtagtaa cgctgtatcc acgcgccagc gttgacagtg ggcttacggc ttcgaggtgc
    11701 gttactgcat taccgaaacg ttcacgcgtg gcgctaagct gtgcgcgcag gatttctgct
    11761 aaacgatatt ccagttgctg aatgcgcgtt tgcgtgcgat gaatcttcgg ctgtggattt
    11821 tgctgattca gccgctgtgt taatcgctgc tgctgttgcc cggcacgctt aagctgattt
    11881 tccagcgcaa agctcatccg tttttgcagg cgttcaagca tggtttgctg gcgtgccagc
    11941 cggagctgcg gatgctgctg ctgcaagcga tgatgaatct gcgtaaagcg acgcgtacgg
    12001 ttggcgagat aataatccat cgccatctcc agccgttgac gggccgattg cacctggcgc
    12061 agtaactctt gctgattacg gctcactact tcagcggcgg cagacggcgt tggcgcacgc
    12121 agatcggcaa caaaatcggc aatggtcaca tccgtctcat gcccgacggc gctgacgacc
    12181 ggaatgcggc tggcaaaaat cgcccgcgct acgcgttcgt cgttaaaact ccataaatct
    12241 tccagcgaac cgccgccgcg cccaacgatc aacacgtcgc actcattgcg ctgattcgcc
    12301 agttcaatgg cgcgaacaat ttgccccggc gcgtcatcgc cctgaacgga ggtggggtag
    12361 atgatcaccg gtagagaagg atcgcgacgt tttaacacat gcaaaatatc atgtagcgca
    12421 gcaccggttt ttgaggtgat cacaccaacg caatgcgcag gggagggaag tggttttttg
    12481 tattgctgat cgaacaaacc ttcagcctgc aacttcgctt tgagctgttc gtacttctgt
    12541 tgcagcagcc cttcaccggc cggctgcata ctctcaacga ttatctggta gtcgccgcgc
    12601 ggctcgtaga gcgtaatatt ggcgcgaact aaaacttgtt gcccatgctg cggacggaag
    12661 gtgacccggc ggttgctgtt gcggaacatc gcgcagcgta cctgggcggt gtcgtctttg
    12721 agtgtaaagt accagtgacc ggaagccggt tgtgtgaaat tagagatttc gccgctgatc
    12781 caaacctgtc ccatctcatg ctcaagcagc agacgaaccg tttgattcag gcgactaacg
    12841 gtaaaaattg caggggattg agaaggtaac atgtgagcga gatcaaattc taaatcagca
    12901 ggttattcag tcgatagtaa cccgcccttc ggggatagca agcatttttt gcaaaaaggg
    12961 gtagatgcaa tcggttacgc tctgtataat gccgcggcaa tatttattaa ccactctggt
    13021 cgagatattg cccatgctac gtatcgctaa agaagctctg acgtttgacg acgttctcct
    13081 cgttcctgct cattctaccg ttctgccgaa tactgctgac ctcagcaccc agctgacgaa
    13141 aactattcgt ctgaatatcc ctatgctttc cgcagcaatg gataccgtaa cggaagcgcg
    13201 cctggctatt gctctggctc aggaaggcgg tatcggcttt atccacaaaa acatgtccat
    13261 tgaacgccag gcagaagaag ttcgccgtgt gaaaaaacac gaatctggtg tggtgactga
    13321 tccgcagact gttctgccaa ccacgacgct gcgcgaagtg aaagaactga ccgagcgtaa
    13381 cggttttgcg ggctatccgg tcgttaccga agaaaacgaa ctggtgggta ttatcaccgg
    13441 tcgtgacgtg cgttttgtta ccgacctgaa ccagccggtt agcgtttaca tgacgccgaa
    13501 agagcgtctg gtcaccgtgc gtgaaggtga agcccgtgaa gtggtgctgg caaaaatgca
    13561 cgaaaaacgc gttgaaaaag cgctggtggt tgatgacgaa ttccacctga tcggcatgat
    13621 caccgtgaaa gacttccaga aagcggaacg taaaccgaac gcctgtaaag acgagcaagg
    13681 ccgtctgcgt gttggtgctg cagttggcgc aggtgcgggt aacgaagagc gtgttgacgc
    13741 gctggttgcc gctggcgttg acgttctgct gatcgactcc tcccacggtc actcagaagg
    13801 cgttttgcag cgtatccgtg aaacccgtgc taaatatccg gatctgcaaa tcatcggcgg
    13861 caacgtggca acagctgcag gtgcacgcgc tttggcagaa gctggttgca gcgcggttaa
    13921 agtcggtatc ggccctggtt ctatctgtac gactcgtatc gttactggcg taggtgttcc
    13981 gcagatcact gccgttgctg acgcagttga agccctggaa ggcaccggta ttccggttat
    14041 cgcagatggt ggtattcgct tctccggcga catcgccaaa gctatcgccg ctggcgcaag
    14101 cgcggtgatg gtaggttcca tgctggcggg tactgaagaa tctccgggtg aaatcgaact
    14161 ctaccagggc cgttcttata aatcttaccg tggtatgggt tccctgggcg cgatgtccaa
    14221 aggttcctct gaccgttatt tccagagcga taacgctgcc gacaaactgg tgccggaagg
    14281 tatcgaaggt cgcgtagctt ataaaggtcg cctgaaagag atcattcacc agcagatggg
    14341 cggcctgcgc tcctgtatgg gtctgaccgg ctgtggtact atcgacgaac tgcgtactaa
    14401 agcggagttt gtacgtatca gcggtgcggg cattcaggaa agccacgttc acgacgtgac
    14461 cattactaaa gagtccccga actaccgtct gggctcctga ttctcttcgc ccgacttcat
    14521 gtcgggcgat ttatattatc tgtttcactt gcctcggaat aagcgtcaat gacggaaaac
    14581 attcataagc atcgcatcct cattctggac ttcggttctc agtacactca actggttgcg
    14641 cgccgcgtgc gtgagctggg tgtttactgc gaactgtggg cgtgggatgt gacagaagca
    14701 caaattcgtg acttcaatcc aagcggcatt attctttccg gcggcccgga aagtactact
    14761 gaagaaaaca gtccgcgtgc gccgcagtat gtctttgaag caggcgtacc gatattcggc
    14821 gtttgctatg gcatgcagac catggcaatg cagttgggcg gtcacgttga agcctctaac
    14881 gaacgtgaat ttggctacgc gcaggtagaa gtcgtaaacg acagcgcact ggttcgcggt
    14941 atcgaagatg cgctgaccgc agacggtaaa ccgctgctcg atgtctggat gagccacggc
    15001 gataaagtta ccgctatccc gtccgacttc gtcaccgtag ccagcaccga aagctgcccg
    15061 tttgccatta tggctaacga agaaaaacgc ttctatggcg tacagttcca cccggaagtg
    15121 actcacaccc gccagggtat gcgcatgctg gagcgttttg tgcgtgatat ctgccagtgt
    15181 gaagccctgt ggacgccagc gaaaattatc gacgatgctg tagctcgcat ccgcgagcag
    15241 gtaggcgacg ataaagtcat cctcggcctc tctggtggtg tggattcctc cgtaaccgca
    15301 atgctgctgc accgtgctat cggtaaaaac ctgacttgcg tattcgtcga caacggcctg
    15361 ctgcgtctca acgaagcaga gcaggttctg gatatgtttg gcgatcactt tggtcttaac
    15421 attgttcacg taccggcaga agatcgcttc ctgtcagcgc tggctggcga aaacgatccg
    15481 gaagcaaaac gtaaaatcat cgggcgcgtt ttcgttgaag tattcgatga agaagcgcag
    15541 aaactggaag acgtgaagtg gctggcgcag ggcaccatct accctgacgt tatcgaatct
    15601 gcggcttctg caaccggtaa agcacacgtc atcaaatctc accacaacgt gggcggcctg
    15661 ccgaaagaga tgaagatggg cctggttgaa ccgctgaaag agctgttcaa agacgaagtg
    15721 cgtaagattg gtctggagct gggcctgccg tacgacatgc tgtaccgtca tccgttcccg
    15781 ggaccaggcc ttggcgttcg tgttctgggt gaagtgaaga aagagtactg tgacctgctg
    15841 cgccgtgctg acgccatctt cattgaagaa ctgcgtaaaa cggacctgta cgacaaagtc
    15901 agccaggcgt tcaccgtctt cctgccagta cgttccgttg gcgtaatggg cgatggtcgt
    15961 aagtatgact gggttgtctc tctgcgtgct gtcgaaacca tcgactttat gaccgcacac
    16021 tgggcgcatc tgccgtacga tttcctcggt cgcgtttcca accgcattat caatgaagtg
    16081 aacggtattt cccgcgtggt gtatgacatc agcggcaagc cgccagctac cattgagtgg
    16141 gaatgatttt gatccctgca ctatgaatga acaaaaccct ccgttactac agagggtttt
    16201 ttatcttcaa gaatcatagg cttgaagtta ctaacatcga ttaattaaac cagctgtccg
    16261 atttgttctc ttctgctttg cccacgcttt tcatcagatc gcgaccgcct tcagtcatat
    16321 ttctgtttgc gtcagcttca gattgcacca catcggtttg cgcagctttg tgcttcagtt
    16381 cctgatcgat aaattcgttt tcgcgcttaa cgcgggcttc ttctttcgcc agcgccagtt
    16441 tttgtttctg aatctctaag ctgcgtagct catcttcata actttgatcg cgttttttgt
    16501 ccgcagtggc ttcggcgtcc agtttatcct gacgagcttt cttatttgcc gctgccgttg
    16561 ccgctctttt gttcgcggcg gcctgggcat ttgcgcgacg ttgcttctct tgctggattt
    16621 ccctgttgcg ctccgcgacc cattcgtcat gctgcctttg ctcttcattt ttaccttgct
    16681 gttccgcttc tgcgacagca gagagttgat cctgcaatga tgaggctata gccggatagc
    16741 ttaaggaggc taagatggcg caaagaaaaa ctttcttcat gactcctcct gattattagc
    16801 tcttttcagg acatttggta tttggctgaa tacgcgtttc gttatacgtt gtggtaataa
    16861 caacggctaa acctgtcgta aactggcact ctttaccctc ctgggtggag gtatacactt
    16921 tggtgccttc cttatatgtt aaagaaacac cttccactaa ggttttatca ttcaccatag
    16981 aacccgctgc cgcgcctaca gctccgccgc caactgcccc tgccgtcgtt ccggaattgc
    17041 tgccagaacc gacgttgtgg ccgataacac cgccagcgac tgcgccaata agcgcgccga
    17101 aggcttgtgc gttccgttta ttttgggagt tgtctacggc aacttttgcg ggaagaatgg
    17161 aaataatatt aacggtttta gtttcttgtt tggtattcag ttgatcggtt tgataaacat
    17221 cggcggcatg atcgtcagca tttgactggc atcctgccag agtgaatgac gctaacattg
    17281 ccacaggcag aagacatttt ttaaatttca tcactattcc ttgttataca cattacgaaa
    17341 aataagacat ttaagatcat caaaagagga aagtaaatgt attgcgtata aagggataga
    17401 ttaaatttat taaaattcac gcagggaata atttataaaa atatatcatt acaacatata
    17461 tattaatttt atatttattt cgcagccaaa caggataaac atcagtcagg acaaagacgt
    17521 aaaatttaag atgaaagaat atctgccgct ttgataatcc caaagagcaa aagcatccga
    17581 cagcgaagtg ccgttatgtt ctgtgaaagt ggagaaggta accgctaacg ttgtaccggt
    17641 gattcgacgg agagtgtgga ttgctgagct tcttttttgc tctgatggtg cagccaggca
    17701 ccgacggacg agtagataaa acggccaaag aagaagataa agctgatgag cagtacgata
    17761 cgggtcatgc gactgttaaa tcggtgtcgt tttcgcatac tagttgcctg actcacaaaa
    17821 ggttccttga agtatgtccc acgctgtgga cggtacttac attaaggcac aacaggacaa
    17881 aatggtcaat tcttcgttat gtaaaaaagc gtcagtggat acatatttta atgttatgga
    17941 agttaattta attatttaca atgatgatgt aataatgatg aattaacatg ataaacgtaa
    18001 taattcatta atcagggtta ttttgtttga tatatatcaa ttggaacttt catatgacac
    18061 ttagaatcaa tgctctctat agtgatgaat aatcatcatt cgaagtcagg tgggatgcct
    18121 gtctgaatac acctccttca ggatgtgggg gattctgctg agcatctatg aaactgaatg
//
LOCUS       DADLXX010000078        17720 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLXX010000078 DADLXX010000000
VERSION     DADLXX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.078"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..848)
                     /locus_tag="JNX27_002564"
     CDS             complement(<1..848)
                     /locus_tag="JNX27_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7883736.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTV"
     gene            856..1356
                     /gene="tnpA"
                     /locus_tag="JNX27_002565"
     CDS             856..1356
                     /gene="tnpA"
                     /locus_tag="JNX27_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7883737.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(1395..1742)
                     /gene="grxD"
                     /locus_tag="JNX27_002566"
     CDS             complement(1395..1742)
                     /gene="grxD"
                     /locus_tag="JNX27_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7883738.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            2077..2904
                     /locus_tag="JNX27_002567"
     CDS             2077..2904
                     /locus_tag="JNX27_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY7883739.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            3032..3613
                     /gene="sodB"
                     /locus_tag="JNX27_002568"
     CDS             3032..3613
                     /gene="sodB"
                     /locus_tag="JNX27_002568"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7883740.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3759..4928)
                     /locus_tag="JNX27_002569"
                     /pseudo
     CDS             complement(3759..4928)
                     /locus_tag="JNX27_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5094..5183)
                     /gene="ynhF"
                     /locus_tag="JNX27_002570"
     CDS             complement(5094..5183)
                     /gene="ynhF"
                     /locus_tag="JNX27_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7883741.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5482..6507
                     /gene="purR"
                     /locus_tag="JNX27_002571"
     CDS             5482..6507
                     /gene="purR"
                     /locus_tag="JNX27_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7883742.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6504..7436)
                     /locus_tag="JNX27_002572"
     CDS             complement(6504..7436)
                     /locus_tag="JNX27_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7883743.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7549..8760
                     /locus_tag="JNX27_002573"
     CDS             7549..8760
                     /locus_tag="JNX27_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7883744.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            9051..10199
                     /gene="cfa"
                     /locus_tag="JNX27_002574"
     CDS             9051..10199
                     /gene="cfa"
                     /locus_tag="JNX27_002574"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7883745.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(10239..10880)
                     /gene="ribE"
                     /locus_tag="JNX27_002575"
     CDS             complement(10239..10880)
                     /gene="ribE"
                     /locus_tag="JNX27_002575"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7883746.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            11095..12468
                     /gene="mdtK"
                     /locus_tag="JNX27_002576"
     CDS             11095..12468
                     /gene="mdtK"
                     /locus_tag="JNX27_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7883747.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12509..13765)
                     /locus_tag="JNX27_002577"
     CDS             complement(12509..13765)
                     /locus_tag="JNX27_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883748.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYCLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            14073..14149
                     /locus_tag="JNX27_002578"
     tRNA            14073..14149
                     /locus_tag="JNX27_002578"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14107..14109,aa:Val,seq:gac)
     gene            14154..14230
                     /locus_tag="JNX27_002579"
     tRNA            14154..14230
                     /locus_tag="JNX27_002579"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14188..14190,aa:Val,seq:gac)
     gene            14338..14643
                     /locus_tag="JNX27_002580"
     CDS             14338..14643
                     /locus_tag="JNX27_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7883749.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14769..16373
                     /locus_tag="JNX27_002581"
     CDS             14769..16373
                     /locus_tag="JNX27_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY7883750.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(16385..17197)
                     /locus_tag="JNX27_002582"
     CDS             complement(16385..17197)
                     /locus_tag="JNX27_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883751.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(17201..17720)
                     /locus_tag="JNX27_002583"
                     /pseudo
     CDS             complement(17201..17720)
                     /locus_tag="JNX27_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
BASE COUNT         4368 a         4568 c         4211 g         4573 t
ORIGIN      
        1 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
       61 attgcgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt
      121 gcgatacagg aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat
      181 ttgaccttcc ggcttaactg tcgctgaagt ctcgccagcg tcttctggtt tttctgaaaa
      241 ctgtttacag gctcaaagac tgtgccatct gacagcgtgg cgagtttagc cacgccagca
      301 tccagcccga tcattgatgc tgaagggtga accggagttg atacttcact ttctgtctga
      361 atactgatgt accacgtacc gcaggactgg ctgacagtga catttttcac aactcccgtg
      421 acctgacggc tgttccggta gcgcatccag ccaagtttcg gcagaaaaat acggctgttt
      481 tcctgatcga gcttaacgcc ctgcgggtag cggaatgcag cattctgtcc ccgctttttg
      541 aatcggggaa aagcagcccg cttccggaag aagtttttgt aagcccgctc aaggtctttc
      601 agtgactgtt gcaatggctg tgagggcgaa tctttaagcc attgcgtttc agtggcgttt
      661 ttccactcaa ccagccagga agccattttc ccgtaaggga tgtatttatt accggcctca
      721 tgattctcat tctgaagcgc cagagcacga ttgaaaacga aacgacatgc gcctgcgaag
      781 cgcctcattt cacgctcctg ttgaccaccg ggtcttaact ggaatttaaa tgcctgtaat
      841 cgtttcacag gtcaaatgat actttggtct gtgaaaaaag aaaccgatat ccggcgtgga
      901 aggcattgtg ttttcctgat gcatgttcac ctggtctttg tcaccagata ccgacgccag
      961 atttttgacc atgacacgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat
     1021 tttgcagctg aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac
     1081 tatcctccca aactggcgat atccagtctg gtaaacagcc tcaaaggcat atcgaatagg
     1141 ttactgcgac gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg
     1201 agtcctggct attttgccag tagctgcgaa ggtgcgccaa tatccgtcat ccgccaatac
     1261 attgaacaac agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa
     1321 ggacgaggtt ttacggcgca ccggataaag gactgacgga tgcccgttcg catccgtcag
     1381 tattgcagga cggattattc cgcgtccggc tcttcagact tgtatttagc ggcagtttct
     1441 ttgatcagct gctgcagttc gccacgctga tacatttcga tcacgatatc acaaccgccg
     1501 accagctcgc cgtcaaccca cagttgcggg aaggtcggcc agttagcata tttcggcagt
     1561 tccgcacgaa tgtccggatt ctgcagaata tcaacatagg caaaacgttc gccacatgcg
     1621 gcaagcgcct ggactgcctg ggcagagaaa ccgcagctcg gcagtttcgg tgaacctttc
     1681 atgtacagca ggatcgggtt ttcagcaatc tggcgttgga ttttttcgat agtggtgctc
     1741 attgtcttgc ttcctcaaac ttctttacgg cagtaatctg acattgtagc gggtcagtgc
     1801 ggcatcggaa aataacattt tcatcacgct tttgctattt tatccctttg ctctatttgt
     1861 tgcatttcaa atattcgttt cttctctgat gcgcggcaaa ctggcgtatt tataaccatt
     1921 tttagctttc actgctgcat ttttttgcgc tcgccaacga aacgtatttt ttaacaataa
     1981 aagctattaa ctttctcttc ttctatgcat tagaatcatc aagttttgta aatcagacgc
     2041 aggcatgata gacctgcctt aacagaggga cgctcagtgg cgcggataaa ccgtatttcg
     2101 atcacgctct gtgctttact ttttaccacc ctgcctttaa cgcctatggc ccatgcttca
     2161 aagcaagcca gggagagttc tgctaccgct catatcacca aaaaagcaga taaaaagaaa
     2221 agcacggcaa ccaccaaaaa gacccagaaa gccagccaga aaacagcgaa aaaagccgcc
     2281 agtaaaagta cgaccaaaag caaaaccgct tcttccgtta aaaaatcttc cattaccgct
     2341 tctaaaaacg ccaaaactcg cagcaaacac gccgtcaata aaacggcctc agccagcttt
     2401 accgaaaagt gtaccaaacg taagggctat aaatcgcatt gtgtgaaagt caaaaatgcc
     2461 gcgtcaggaa cccttgccga cgcgcacaaa gcgaaggtgc aaaaagccac gaaagtggca
     2521 atgaataaac tgatgcagca aattggtaag ccatatcgtt ggggcggcag ctcaccgcgt
     2581 accggttttg attgcagcgg cctggtttat tacgcttata aagatttggt gaaaattcgt
     2641 attccgcgta cggcgaatga aatgtatcac ctgcgtgatg caggacctat cgaacgtagt
     2701 gagctgaaaa acggcgacct ggtctttttc cgtactcagg gacgcggtac agccgatcat
     2761 gtcggcgtgt atgtcggcaa cggcaaattt attcagtcac cgcgcacagg tcaggaaatt
     2821 caaatcactt ctctcagtga agactactgg cagcgccact atgttggcgc tcgtcgggta
     2881 atgaccccaa aaacacttcg ctaaaacttt accctgttgt tacggcaaca gggtaagttc
     2941 atcttttgtc tcacctttta atttgctacc ctatccatac gcacaataag gctattgtac
     3001 gtatgcaaat taataataaa ggagagtagc aatgtcattc gaattacctg cactaccata
     3061 tgctaaagat gctctggcac cgcacatttc tgcggaaact atcgagtatc actacggcaa
     3121 gcatcatcag acttatgtca ctaacctgaa caacctgatt aaaggtaccg cgtttgaagg
     3181 taaatcactg gaagagatta ttcgcagctc tgaaggtggc gtattcaaca acgcagctca
     3241 ggtctggaac catactttct actggaactg cctggcaccg aacgccggtg gcgaaccgac
     3301 tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc tttgccgatt tcaaagcgca
     3361 gtttactgat gcagcgatca aaaactttgg ttctggctgg acctggctgg tgaaaaacag
     3421 cgatggcaaa ctggctatcg tttcaacctc taacgcgggt actccgctga ccaccgatgc
     3481 gactccgctg ctgaccgttg atgtctggga acacgcttat tacatcgact atcgcaatgc
     3541 acgtcctggc tatctggagc acttctgggc gctggtgaac tgggaattcg tagcgaaaaa
     3601 tctcgctgca taataactga tggcaaatgc agcattgcct gatgcgcttc gcttaccatg
     3661 ccaacgcgga tgaggcgtta acagcgcatt cggcaagata aatcgcacgt tgtcagcaac
     3721 tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt agctgttagc aacgcaaact
     3781 gtttcaggtt gttttctggc tgacataaac accagcaata atgccagtcc cgcgacaatc
     3841 gcccccgtca ccggcacaaa gctgtatccc agcccagcgg aaattaccgc accaccagca
     3901 gctgctccca gcgcatttcc aagattaaag gcaccaatat tgactgatga agacagacct
     3961 ggcgcttcac tggcgacatg catcacgcgc atctgtaacg gaggtacaac cgcaaaggtt
     4021 gctgcgcccc acaccaccat gctaatagct gcgccgaact cattgcgggc caggaacggg
     4081 attgccagca taatcaccat cagcagcaac aaaaagcctt tcaacgtgcc gttaactgaa
     4141 cgatctgcca gtttgccgcc gagatagtta ccgatagaga atccgacacc aatcagcacc
     4201 agcattgccg tgacgaacac cggtgttgcg tgggtaatac tttgcagtac cggagagata
     4261 taggtgtaga gggtaaacat tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc
     4321 acctgcggac gcattaatac cgccagctct tttttcactt caggtcgtgc ccctgtacca
     4381 cctttaggta atgagaagaa cagacttacc attgaaatca ctcccagccc cgccgttgcc
     4441 agaaatgaca tccgccagcc gatggtttca cccaacctgg tcgccgccgg cacgccaccg
     4501 atatttgcca gggttaaccc cataaacata gtggcaactg cgctggcctg tttatgtttt
     4561 ggcaccacgc ttgcggccac gactgaaccc aaaccaaaaa atgctcagtg attcaggctg
     4621 gtcaaaatac gtgaaagcat cagggtcata taatccggcg cgatggcgga aagaacgttg
     4681 cctagcgtga aaattgccat caggaaaatc aacgcactgc ggcgggcacg atgagaaagt
     4741 agaagcgtca tcagcggcgc gcccaccatt acgccaactg cataggcact gattaacatt
     4801 ccggcagcgg gaatcgagac atccacaccg cgcgcaatga cgggcaacaa gcccattggc
     4861 gagaactccg ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa cggatagtta
     4921 attttcatgc cttatctcca cctcttcgcg tcattacgcg atattcatta aagtggcgaa
     4981 agcatgacag caatcacaaa aaaatgaaaa taacaaaaag agaaaacact tttgccattt
     5041 tgctaacaaa caggaaggag atgcgaggga gaacgcgctc cctcgagagg aaatcagtgc
     5101 agcgcggcag tcaaacccac ggctacgatc aaaccgagga cgataatcgt tgttaccagt
     5161 gaaaatttaa ggtcggtgct catcaagttt tctccttttt tattaccaca caaaaaatga
     5221 tattacgcat ttttacacac tgtgatgaaa aaatctcccg tcatttataa tgataagtgt
     5281 ttttaccact tccccttttc gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt
     5341 actggccatt gaccccttcc tgacgctccg tgtcgttttt ccggcgtacc gcaacacttt
     5401 tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc gattttgcag gagctgaagt
     5461 tagggtctgg agtgaaatgg aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt
     5521 tccactacaa ctgtgtcaca cgtgatcaac aaaacacgtt tcgtcgctga agaaacgcgc
     5581 aacgccgtgt gggcagcgat taaagaatta cactactccc ctagcgcggt ggcgcgtagc
     5641 ctgaaggtta accacaccaa gtctatcggt ttgctggcga ccagcagcga agcggcctat
     5701 tttgccgaga tcattgaagc agttgaaaaa aattgcttcc agaaaggtta caccctgatt
     5761 ctgggtaatg cgtggaacaa tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa
     5821 aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg
     5881 ctggaagagt atcgccatat cccaatggtg gtgatggact ggggtgaagc aaaagctgac
     5941 ttcaccgatg cggtcattga taacgcgttc gaaggcggct acatggccgg gcgttatctg
     6001 attgaacgcg gtcaccgcga aatcggcgtc atccccggcc cgctggaacg taacaccggc
     6061 gcaggccgcc ttgccggttt tatgaaggcg atggaagaag cgatgatcaa ggtgccggaa
     6121 agctggattg tgcagggtga ctttgaacct gaatccggtt atcgcgccat gcagcaaatc
     6181 ctgtcgcagc cgcatcgccc tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc
     6241 gcactttgtg ctgctgatga aatgggcctg cgcgtcccgc aggatgtttc gctgatcggt
     6301 tatgataacg tgcgcaacgc gcgctatttt acgccggcgc tgaccacgat ccatcagcca
     6361 aaagattcgc tgggtgaaac agcgttcaac atgctgttgg atcgtatcgt caacaaacgt
     6421 gaagaaccgc agtccattga agtgcatccg cgtttgattg aacgccgctc cgtggctgac
     6481 ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg gagtctcttc cggctcccgc
     6541 agccactcct tattcagcgt ctcactatcg ccgagatact caagcagcca ggttaacgca
     6601 ggcgacatat cattttgctg ccatgtcaga caacatgccg aatccgggaa ggggttttcc
     6661 agttcaagtg ctacccactt cccctcatta agccacggtt tggcgaaatg tgttggcacc
     6721 atccctatgc ataatcctgc cgagatacag gttgccgatg attcccagtc aggtacgaca
     6781 agtctttttt ggttatccag caaccaggta atacgtttag gtagcgttcg cgaggtgtct
     6841 tcgcgcacca acgacggcca gttgcgcaac gtatcatcgc tgaacgggcc atccatcaac
     6901 gccagcgggt ggtgactggc aacaacgcaa ctccagctta gcatccccat atcccggaag
     6961 gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa ttgccagttc cacgcgcccg
     7021 tcggaaagcg catcccagac accgttgaac acttcctgaa agacaagaag ttcaacatca
     7081 tcaaaatggc gataaaaatc aacgatcatc tgccgtgtac gttctggcct gacaatatta
     7141 tccactgcga tagctaactg accgcgccag ccgttcgcta tctgctgaca ttgctggcgg
     7201 gtgatctgca tttttttgac aacagagcgc ccttctttga gaaaccacgc tccagcagcg
     7261 gtcagctcca catcacggtg ccgtcgttca aagagcggta ccgccagcca ctcttccagc
     7321 tgacgcacgg tatagctgac cgcagaagga acgcgatgca gctcctgtgc cgcagcgcta
     7381 aaactaccat tacgcgctac cgcatcaaca acttcgagtg aatattctga ccacatagtc
     7441 agcctgcaaa atttttgaaa ccagtcatca aatattaccg tttcacaaca ctaatttcac
     7501 tccctacact ttgcggcggt gtttaattga gagatttaga gaatatacat gcaacctggg
     7561 aaaagatttt tagtctggct ggcgggtttg agcgtactcg gttttctggc aaccgatatg
     7621 tatctgcctg ctttcgccgc catacaggcc gacctgcaaa cgcctgcgtc tgctgtcagt
     7681 gccagcctta gtctgttcct tgccggtttt gccgcagccc agcttctgtg ggggccactc
     7741 tccgaccgtt atggtcgtaa accggtgtta ttaatcggcc tgacaatttt tgcgttaggt
     7801 agtctgggga tgctgtgggt agaaaacgcc gccacgctgc tggtattgcg ttttgtacag
     7861 gctgtggggg tctgcgccgc ggcggttatc tggcaagcat tagtgacaga ttattatcct
     7921 tcacagaaag tcaaccgtat ttttgcgacc atcatgccgc tggtgggtct gtctccggca
     7981 ctggctcccc tgttaggaag ctggctgctg gtccattttt cctggcaggc gattttcgcc
     8041 accctgtttg ccattaccgt ggtgctgatt ctgcctattt tctggctcaa acccacgacg
     8101 aaggcccgta acaatagtca ggatggtctg acctttaccg acctgctacg ttctaaaacc
     8161 tatcgcggca acgtgctgat atacgcagcc tgttcagcca gtttttttgc atggctgacc
     8221 ggttcaccgt tcatccttag tgaaatgggc tacagcccgg cagttattgg tttaagttat
     8281 gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct gtcgcaccgc actgcagaaa
     8341 tggcaaggca agcagttatt accgtggttg ctggtgctgt ttgctgtcag cgtcattgcg
     8401 acctgggctg cgggcttcat tagccatgtg tcgctggtcg aaatcctgat cccattctgt
     8461 gtgatggcga ttgccaatgg cgcgatctac cctattgttg tcgcccaggc gctgcgtccc
     8521 ttcccacatg caaccggtcg cgccgcagcg ttgcagaaca ctctgcaact gggtctgtgc
     8581 ttcctcgcaa gtctggtagt ttcctggctt attagtatca gcacgccatt gctcaccacc
     8641 accagcgtga tgttatcaac agtagtgctg gtcgcgctgg gttacatgat gcaacgttgt
     8701 gaagaagttg actgcccgaa tcatggcaat gccgaagtcg ctcatagcga atcacactga
     8761 tctatatcga tatacttata cttaggctgc taacaaaatt ttgttgtatg attgaaatta
     8821 gcggcctata ctaatttcga gttgttaaag ctacgataaa tattatgttt ttacggggac
     8881 aggatcgttc ccgactcact atggatggtc atttcggcaa gggttcctcc tttccctctg
     8941 ttctacgtcg gattatagac tcgcggtttt ttctgcgaga tttctcacaa agcccaaaaa
     9001 gcgtctacgc tgttttaagg ttctgatcac cgaccagtga tggagaaact atgagttcat
     9061 cgtgtataga agaagtcagt gtaccggatg acaactggta ccgtatcgcc aacgaattac
     9121 ttagccgtgc cggtatagcc attaacggtt ctgccccggc ggatattcgt gtgaaaaacc
     9181 ccgatttttt taaacgcgtt ctgcaggaag gctctttggg gttaggcgaa agttatatgg
     9241 atggctggtg ggaatgtgac cgactggata tgttttttag caaagtctta cgcgcaggtc
     9301 tcgaaaacca actcccccat catttcaaag acacgctgcg tattgccggc gctcgtctct
     9361 tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa ggagcattac gatttgggta
     9421 atgacttgtt cagccgcatg cttgatccct tcatgcaata ttcctgcgct tactggaaag
     9481 atgccgataa tctggaatct gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc
     9541 agttaaaacc agggatgcgc gtactggata ttggctgcgg ctggggcggt ctggcgcact
     9601 acatggcatc taactatgac gtaagcgtgg tcggcgtcac catttctgcc gaacagcaaa
     9661 aaatggctca ggaacgctgt aaaggcctgg atgtcaccat tttgctgcaa gattatcgtg
     9721 acctgaacga ccagtttgat cgtattgttt ctgtggggat gttcgagcac gtcggaccga
     9781 aaaattacga tacctatttt gcggtggtgg atcgtaattt gaaaccggaa ggtatattcc
     9841 tgctccatac tatcggttcg aaaaaaaccg atctgaatgt tgatccctgg attaataaat
     9901 atatttttcc gaacggttgc ctgccctctg tacgccagat tgctcaatcc agcgaacccc
     9961 actttgtgat ggaagactgg cataacttcg gtgctgatta cgatactacg ttgatggcgt
    10021 ggtatgaacg attcctcgcc gcatggccag aaattgcgga taactacagt gaacgcttta
    10081 aacggatgtt tacctattat ctgaatgcct gcgcaggtgc tttccgcgcc cgtgatattc
    10141 agctctggca ggtggtgttc tcacgcggtg ttgaaaacgg ccttcgagtc gctcgctaaa
    10201 ggctattcta tcgccccctc ttcgggggcg agttcagatc aggcttctgt acctggttga
    10261 ttcatggcat tttctcgtgc cgccagcaca cgttctaccg tatctaccac tgcctgagtt
    10321 tgtggatcga tttcaatgtt gacgcgtgcg ccaagttttt tcttcccaag agtcgtgcgt
    10381 tccagtgttt ccggaattaa atggacgcaa aaacgcgttg gcgtgacttc gccgacggtc
    10441 aggctaatac cgtcgatgcc aataaatcct ttgtacagga tatatttcat caactgacta
    10501 tcctggactt taaaccagat ctggcgatta ttttccgagg ttaatatttt cgccacttca
    10561 gcggtggtca taatatgacc tgacattaag tgtccgccaa tttcatcact gaatttcgcc
    10621 gcacgctcaa cgtttaccca atcccccact tttaaatcgc caagattggt aatgcgtaac
    10681 gtttctttca tcaggtcaaa actgacatgg ttgccgttaa tttccgtcac ggtcaggcag
    10741 caaccgttat gcgccacgga agcaccggtt tccaggccgt ccagcatgtg gtcgggtaac
    10801 tccaccacat gcgtacgaaa atttggtttc tcgtcaatcg acaccagttt tgcggtgccc
    10861 tgtacaatcc ccgtaaacat acttacaact cctgaaatca gttaagacat tctgttcagc
    10921 acaatagcag gtggaaaacg cccttaccag tgaaggggta agaatggcta ttttttcact
    10981 ggagaattaa taaatcctcg ctacaataga ctgaatttcc cctgcttctt ctttttgctg
    11041 cccattcagg cggcttttta gtctctcata taactacaaa taaaaggtgt tcacgtgcag
    11101 aagtatatca gtgaagcgcg tctgttatta gcattagcaa tcccggtgat tctcgcgcaa
    11161 atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg cgggcggcta tagtgccacc
    11221 gacatggcgg cggtcgctat cggtacttct atctggcttc cggcgatcct ctttggtcac
    11281 ggactgctgc tggcattaac gccggttatc gcgcaattaa atggttccgg tcgacgtgag
    11341 cgcattgcgc atcaggtgcg acaaggtttc tggctggcag gttttgtttc cgttctcatt
    11401 atgctggtgc tgtggaatgc aggttacatt atccgctcca tggaaaacat cgatccggct
    11461 ctggcggaca aagccgtggg ttatctgcgt gcgttgttgt ggggcgcgcc gggatatctg
    11521 ttcttccagg ttgcccgtaa ccaatgtgaa ggtctggcaa aaaccaagcc gggtatggta
    11581 atgggcttta tcggcctgct ggtgaacatc ccggtgaact atatctttat ttatggtcat
    11641 ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg ctactgcggc ggtgtattgg
    11701 gtcatgttcc ttgccatggt ttcttacatt aaacgcgccc gctccatgcg cgatattcgt
    11761 aacgaaaaag gcaccgctaa gcccgatcct gcggttatga aacgactgat tcaactcggt
    11821 ttgccgattg cgctggcact gttctttgaa gtgacactgt ttgccgtcgt ggctctgtta
    11881 gtgtctccgc tcggtattgt tgatgtcgca ggacaccaga ttgccctgaa ctttagttca
    11941 ctgatgtttg tgcttccaat gtcgctggcg gcagcggtaa ctatccgcgt aggttatcgt
    12001 ctgggtcagg gctcaacgct ggatgcgcaa accgctgcgc ggaccgggct tatggtgggt
    12061 gtctgtatgg caaccctgac ggccattttc acggtttcac tgcgggagca aatcgccctg
    12121 ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc atttgatgtt gctggcggcg
    12181 gtatatcaga tttctgactc aatccaggtg attggcagtg ggattttgcg tggttataaa
    12241 gatacgcgtt ccattttcta tatcaccttt acagcttact gggtgctggg cttgccaagc
    12301 ggctatattc tggcactgac cgatctggtc gttgaaccta tggggccagc aggcttctgg
    12361 ataggcttta ttatcggcct gacatcggca gccattatga tgatgctgcg tatgcggttc
    12421 ctgcaacgta tgccgtcagc catcattctg caacgagcgt cccgctaata aagacaaggc
    12481 gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg gagcgttgcg ccaatcccgt
    12541 cttcgtctgg ctgtaatttc agagcgttac acagagtttg ccgactggca tctgttatct
    12601 caacgcgtat atccccgtta acttcccctt tcacattgat gaagtcaggt gaaatttctt
    12661 tcgcactggt ttgcaggcaa atcgctccct ctccgcgcag gcgtttcact ttcagttgca
    12721 catgcgttgc cgcaccagtg atcagccaca gttttccgcc attcagattg atgttaccaa
    12781 caatgaaagt gacgctgtca ccttttaccg tactgccgtc gagaatcaac acaccgccct
    12841 gctgtacagt aatcgttgat tgttgcagct ctgcgccata accaataatc attgaagcgc
    12901 cgtccagtaa tgcaacactc ccggtcagtt caaccggtgc gcgatcttcc tctacgacaa
    12961 aattttcgtc cgcgctggtg ggtgacaaca ggatcactgt tccaccttgt attaccgcat
    13021 tttccagcgt accggcatgc acttcagccc gatcccccac attcaccgac aggttttgcg
    13081 tcttaccgga actgtagagc tgaaggccat ccgtccccag acaataaatg gatccattct
    13141 caacaatagt gtcagagtct tcaccaccga gattattcat ggtgccacca gcaagcaacg
    13201 tatcactggc tttcccggcc agcataaaaa cgccgccctg attaatctca caattgctgg
    13261 cttctccact tggatacacc aacaattcac caccttcatc taccacgacc gctgaggttg
    13321 tcccattaac caacaggccg ccctcttgat cgagaatgat tttttccgcg cgatgtcctt
    13381 ccagtacacg caggttaccg ccattttcca gcaacaaacc gtaggcataa ccctgatcaa
    13441 cgctgaattc accttcggga tggcggccat taaccgtagc gagcgtagag agactaattg
    13501 cgcctccctc ggactgaaca atatctgtgg caatggcacc gtcgctgacc gatagcgtgc
    13561 cattgttacc gtactgtgtg catttggcga tcccaccggt tgccactttc aaccagccag
    13621 cccccgtgac cacggtattt tcggctcttc cgccagcttc aacaatcaac tcgccttcag
    13681 taagttgcgt cgcgccaatg acctcgccgg tcttaacaat ttgcacattc ccgtcgctca
    13741 tcaagttttt cgcatcagat cccatgattt attcctttgc tgcatctgtg tgcctttatt
    13801 gctacctaag tgtaaaggct acggaggatt tatccacgac agatttgaga tggtggcaaa
    13861 caactctgtt taaactctga tacacgaatt attgggttgt atcagatgta aatgcgatcc
    13921 tgaataaaaa tcgcccttgc aaatcaacaa aatatagcca atttgcttaa aagctctgca
    13981 aacggtcgaa atagcgcaga aaattacgtt ttgcctcttg ccacctttcc actctgccgc
    14041 taatattcgt ccccgttgtc acctacaacg ttgcgttcat agctcagttg gttagagcac
    14101 caccttgaca tggtgggggt cgttggttcg agtccaattg aacgcaccat cctgcgtccg
    14161 tagctcagtt ggttagagca ccaccttgac atggtggggg tcggtggttc gagtccactc
    14221 ggacgcacca gattttctta atctggtctt ttcctttttc cctctgtttc ttctctgtat
    14281 ccaatacgtt aaaagattta cactgtcttc gtctgcgtta tcagaaggag aatcgctatg
    14341 gcaactttgt tacaatttca ttttgctttt aatggcccat ttggcgacgc aatggctgag
    14401 cagcttaaac cacttgctga gtcgattaat caggaacctg gttttctgtg gaaggtatgg
    14461 acagaaagtg aaaagaacca cgaagccggt gggatctacc ttttcactga tgaaaaaagc
    14521 gcccttgcct atctggaaaa acatactgcc cgactgaaaa atctcggcgt tgaggaagtt
    14581 gtcgccaaag ttttcgatgt caatgagcca cttagtcaaa tcaatcaggc aaaactcgcc
    14641 tgacagaatt taatcaaggg cggttagcgc ccttttcatc cctgtctgaa atttctcaaa
    14701 ttctaaaaat ctcaaccaaa cttatctgat aacactaaat tcgaaagaat gcgtacaggt
    14761 aagtaacaat gaaaaaaatt gctattgtgg gtgccgggcc tacggggatc tacaccttat
    14821 tctcgcttct acagcaacaa actccacttt ctatttctat cttcgagcag gctgacgagg
    14881 ccggtgtcgg gatgccatac agtgatgagg aaaactcaaa aatgatgctg gcaaatattg
    14941 ccagtattga aataccgccg attaattgta cgtatctcga atggctacaa aagcaagaag
    15001 ccagccatct ccagcgttat ggcgttaaaa aagaaacctt gcacgatcgt cagtttttac
    15061 cgcgaattct gctgggcgaa tatttccgcg atcaattttt acgattagta gaccaggcac
    15121 gacagcaaaa atttgcagtg gctgtttatg aatcatgcca ggttaccgat ctgcaaatta
    15181 caaatgctgg cgtcatgctc gctacaaatc aggatttacc cagcgagacg tttgatttag
    15241 cggtgattgc cacgggtcac gtctggcctg atgaagaaga agcaaccaga acgtattttc
    15301 ccagcccgtg gtcaggcctg atggaagcaa aggtcgatgc gtgtaacgtg ggtattatgg
    15361 gaacatcctt gagcggactg gatgcggcaa tggcagtggc tattcagcat ggttcgttca
    15421 ttgaagatga taaacaacac gtcgtttttc accgcgataa cgcaagtgaa aagctaaata
    15481 ttacgttgat gtcgcgcacg ggtattttac ccgaagccga tttctattgc cctattccct
    15541 gcgagccctt acacatcgtc accgatcagg cattaaatgc tgagattcaa aaaggtgaag
    15601 agggcctttt ggatcgggta tttagattga tagtagagga aatcaagttt gctgatccag
    15661 actggagcca acgcatagcc ttagagagcc tgaatgtcga ttcctttgct caagcctggt
    15721 ttgccgagcg caaacaacgc gacccatttg actgggcaga aaaaaatctc caggaagtcg
    15781 aacgcaataa acgagaaaaa catactgttc cctggcgtta tgtcattctg cgcctgcatg
    15841 aagccgtaca ggaaattgtt cctcatctga atgaacacga ccataaacgg ttcagtaaag
    15901 gccttgccag ggttttcatc gataattatg cggcaatccc ttcagagtct attcgtcgac
    15961 tactggcctt acgtgaagcg gggatcattc atattctcgc cctcggtgaa gactacgaaa
    16021 tggaaattaa tgagtcgcgc accgtcctga aaacggaaga caacagctac tcgtttgacg
    16081 tttttattga tgcccgcgga cagcgtccgc ttaaagtgaa agatattcct ttccctgggc
    16141 tacgcgagca attacagaaa acaggggatg aaatccctga tgttggcgaa gattatacgt
    16201 tacagcaacc cgaagatatt cgtggacgcg tagcgttcgg cgcgttgccc tggctgatgc
    16261 acgaccagcc tttcgttcag ggacttacgg catgtgcaga aattggtgag gcgatggctc
    16321 gggcggtcgt aaagcctgca tcccgtgctc gtcggcgtct ttcgtttgat taaaggtaaa
    16381 gctatcagca ctcaacaaac tgatgctgtt cattaagacg atagcggact ccggttcgaa
    16441 tattgttttc accttcaatg gcgacggcga agcgaactct ttcgccgtca tgatatgcca
    16501 gtgcggcgct tccccctagc ccgagaataa ttgagtcgac cacgcccgtg ctcactaccg
    16561 ttgaattttc accgctggca atgatgtgta cattgtcgcc gctgttggca attctggcgt
    16621 ttgcgccaaa gctggcgatt tgcaccaggt caccattact ggcgacatga caccgttcac
    16681 ccaatgtaca aacgcgtact cgcattccgg tattcgcaat ccgactacta tctccagcgc
    16741 tgcttatccg cgaagaatta ccggttacag caacccgtgc gcgttcaccg gagctgccaa
    16801 tgtgactgtt aaagccgaca cttccaatcc gcacgctata acccatactg gcaatttgcg
    16861 cggcataccc agcggttgcg attcgggcgt tatcctcaca acagccgatg cgagcatgat
    16921 cgtcgctgcg ggcaaactga ctgcaaaatg gtatctctcc ggggtctgtg gccagttttt
    16981 gcatagagga aacttcctga tgcgcgaagt tttcatccgc cagccatttt ttccacgcat
    17041 actcaaccag actctccgcc cagtccgtat atccttcctc aatcagtgca tgacgaatat
    17101 cggcataact accgccgcgt gggaaatggc gcaggaacca gcgatacatc accgctccga
    17161 tacgccattc tcgcaggtct gctcgtgtaa tgatcattcg ttagtgccgg tgatagccat
    17221 cgaccatgct tttaaaggtt tcgtgtggcg tacgccccgt ggtgcaaaga taaagatgac
    17281 cgaagataaa aaagaggctt ataaatgcca gagcaaaatg cgcctgcaat aaccagtatc
    17341 ttacgccagg aaacacatct cccacggctt gcggatagag acacaacagc cccgtcaata
    17401 gtaacaacgg cagcaatcca tacatgacac caacataggc gacctgctgt aagggattaa
    17461 atttagactg tgttgttgcc gggaaaggat gttcttcccc ctgcataatg ccaaacaaat
    17521 aaaatcgcgt ttgttttgcc gctcgttcca gccacccctg acgacgaatg cgatagtggt
    17581 gaccattatc cccaacggca ttgatcagca caaagccgag ccagcatgcc agtaacaaaa
    17641 atcagcaaac ttcatgcacc gcaaccagac ttttaaccgc agttgcgccc accatcgcaa
    17701 agtgatttat cagcccactt
//
LOCUS       DADLXX010000079        17649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLXX010000079 DADLXX010000000
VERSION     DADLXX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.079"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            144..473
                     /locus_tag="JNX27_002584"
     CDS             144..473
                     /locus_tag="JNX27_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7883752.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            721..966
                     /gene="nrdH"
                     /locus_tag="JNX27_002585"
     CDS             721..966
                     /gene="nrdH"
                     /locus_tag="JNX27_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7883753.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            963..1373
                     /gene="nrdI"
                     /locus_tag="JNX27_002586"
     CDS             963..1373
                     /gene="nrdI"
                     /locus_tag="JNX27_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7883754.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1346..3490
                     /gene="nrdE"
                     /locus_tag="JNX27_002587"
     CDS             1346..3490
                     /gene="nrdE"
                     /locus_tag="JNX27_002587"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7883755.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLQEAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3500..4459
                     /gene="nrdF"
                     /locus_tag="JNX27_002588"
     CDS             3500..4459
                     /gene="nrdF"
                     /locus_tag="JNX27_002588"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7883756.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            4814..6016
                     /locus_tag="JNX27_002589"
     CDS             4814..6016
                     /locus_tag="JNX27_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7883757.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6009..7073
                     /gene="proW"
                     /locus_tag="JNX27_002590"
     CDS             6009..7073
                     /gene="proW"
                     /locus_tag="JNX27_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7883758.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7131..8123
                     /gene="proX"
                     /locus_tag="JNX27_002591"
     CDS             7131..8123
                     /gene="proX"
                     /locus_tag="JNX27_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7883759.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8315..9494
                     /locus_tag="JNX27_002592"
                     /pseudo
     CDS             8315..9494
                     /locus_tag="JNX27_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9618..10355
                     /locus_tag="JNX27_002593"
     CDS             9618..10355
                     /locus_tag="JNX27_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7883760.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10345..10680
                     /gene="ygaH"
                     /locus_tag="JNX27_002594"
     CDS             10345..10680
                     /gene="ygaH"
                     /locus_tag="JNX27_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7883761.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            10771..11301
                     /gene="emrR"
                     /locus_tag="JNX27_002595"
     CDS             10771..11301
                     /gene="emrR"
                     /locus_tag="JNX27_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7883762.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMN"
     gene            11428..12600
                     /gene="emrA"
                     /locus_tag="JNX27_002596"
                     /pseudo
     CDS             11428..12600
                     /gene="emrA"
                     /locus_tag="JNX27_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12617..14155
                     /gene="emrB"
                     /locus_tag="JNX27_002597"
     CDS             12617..14155
                     /gene="emrB"
                     /locus_tag="JNX27_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7883763.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNX27_002598"
     CDS             complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNX27_002598"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7883764.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNX27_002599"
     CDS             complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNX27_002599"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7883765.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16513..16941)
                     /locus_tag="JNX27_002600"
     CDS             complement(16513..16941)
                     /locus_tag="JNX27_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7883766.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNX27_002601"
     CDS             complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNX27_002601"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7883767.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVHPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4183 a         4592 c         4495 g         4379 t
ORIGIN      
        1 ctcgttattt tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
       61 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      121 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      181 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
      241 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt
      301 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      361 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      421 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
      481 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
      541 tgaatcacaa aaatccccct cccccgtcac gctcatatcc agggtaattt cgatcactat
      601 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
      661 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacaga aatacgaatc
      721 atgcgcataa ctatttatac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
      781 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
      841 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
      901 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
      961 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     1021 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1081 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     1141 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     1201 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1261 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     1321 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     1381 aacgatggat taccacgcgc tgaatgcgat gcttaatctt tacgatagcg caggtcgcat
     1441 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
     1501 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     1561 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     1621 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     1681 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     1741 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
     1801 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     1861 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
     1921 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     1981 gaatctgcag gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     2041 tccggtgatg aaaatgttgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     2101 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
     2161 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2221 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     2281 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccattagcg aacactatga
     2341 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2401 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacagt
     2461 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
     2521 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2581 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgatttttc
     2641 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     2701 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     2761 gaatttacat ggttatctgg cgagggaagg catcgcttat ggttcgccgg aagccctgga
     2821 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     2881 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     2941 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     3001 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     3061 cgtgatgcgc tacggcatat acaaccagaa tctccaggcg gtgccgccaa ccgggtctat
     3121 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3181 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3241 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
     3301 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3361 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     3421 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     3481 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     3541 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3601 aaaggtgccg ctgtcgaacg atattcccgc ctggcagaca ttaactgtcg tagaacaaca
     3661 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     3721 cgcgccttcg ctgatgcccg atgcgctcac gcctcatgaa gaggcggtat tatcgaatat
     3781 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     3841 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     3901 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     3961 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4021 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
     4081 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     4141 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
     4201 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4261 ctgttacaac accaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4321 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4381 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     4441 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
     4501 taattcccgc caaatagctt tttatcacgc aaatattctg tagtgatcta cactgatact
     4561 ctgttgcatt attcgcctga aactacaata ttcaggcgtt ttttcgctat ctatgacaaa
     4621 aaatatcaac ttttctcgat ttgctctcag cccttatatc acggaaaatt ccggcgattt
     4681 gctcgcatca atattcatgc cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt
     4741 tagggtagaa aaaagtgact attcccattg ggtaatatat cgacataaac aaataaagga
     4801 atcattctat tgcatggcca ttaaattaga aattaaaaat ctttataaaa tatttggtga
     4861 gcatccacag cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga
     4921 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     4981 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     5041 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     5101 cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     5161 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     5221 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     5281 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     5341 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
     5401 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     5461 caccattgtc tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc
     5521 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     5581 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tgttcagtgc
     5641 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaactcctg gcttcggccc
     5701 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     5761 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     5821 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     5881 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     5941 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     6001 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     6061 cgcaatccgc agacgcctgg gggacaccga cgactgcacc ggctgacggc ggtggtgctg
     6121 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     6181 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     6241 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc
     6301 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     6361 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ctcgctgatt gccatcggcg
     6421 caatcggtgc ctgttcgcag gcaatggtga ctctggcgct ggtgttaact gccctgctgt
     6481 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     6541 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     6601 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     6661 cgctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     6721 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     6781 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     6841 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     6901 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     6961 ttatcctcga tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     7021 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     7081 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     7141 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc
     7201 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     7261 agacgctgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg
     7321 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     7381 tgaactggac gccactccat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     7441 atcgtgaagg ggtatttgtt aacggcgcag cacagggtta cctgatcgat aagaaaaccg
     7501 ccgaccaata caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     7561 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     7621 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     7681 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     7741 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     7801 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     7861 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     7921 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     7981 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     8041 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     8101 aggcgctggc agcgcagaag taatttttat tcgggcggat aaggcgtttt cgcctcatcc
     8161 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ctatcatgcc tacaaaccac
     8221 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
     8281 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     8341 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     8401 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     8461 ctttattgtt accgccgcgc agttgggcta tgccgcaggt ctactgtttc ttgttcccct
     8521 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     8581 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     8641 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     8701 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     8761 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     8821 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
     8881 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgttcatcag
     8941 cgataagatc ctgcgcaccc gcgcgttgct gggctgcatg acctttgcca acttcagcat
     9001 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtga
     9061 cattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     9121 ttttgccgac aagggcaaat cgcaccacaa ctttcggtct gcttctgctg ttactttcat
     9181 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattatcggc attctggtgc
     9241 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
     9301 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
     9361 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
     9421 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
     9481 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
     9541 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
     9601 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
     9661 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
     9721 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
     9781 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
     9841 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
     9901 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
     9961 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    10021 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    10081 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    10141 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    10201 gcaaacaatc tctttgcgtt accgcagctt tagttggtgc ccttgcaggc gtaacgctat
    10261 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    10321 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    10381 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    10441 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    10501 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    10561 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    10621 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    10681 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    10741 atcgactgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    10801 atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat ccttctgact
    10861 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    10921 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    10981 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    11041 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    11101 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    11161 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    11221 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    11281 gttctcgaag cgatgaacta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    11341 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    11401 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    11461 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    11521 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca tttcgaagaa
    11581 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    11641 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt tctggtcact
    11701 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    11761 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    11821 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    11881 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    11941 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    12001 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctagctg
    12061 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtttcccg tcgtgccgtg
    12121 caaccgggtg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    12181 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat cggtcagccg
    12241 gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    12301 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    12361 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    12421 caatacccgc tgcgcattgg tttgtccacg ctggtaagcg tcaataccac taaccgtgac
    12481 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    12541 atcagcctag cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    12601 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    12661 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    12721 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccaaggaa cgtgggtaat
    12781 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    12841 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    12901 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    12961 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    13021 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    13081 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    13141 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    13201 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    13261 cctgcagatt atgctcgacc gcggtaaaga gctggactgt ttttcatcac aggaaattat
    13321 cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    13381 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    13441 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    13501 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    13561 tattccggtg attctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    13621 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    13681 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    13741 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    13801 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    13861 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    13921 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    13981 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    14041 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    14101 gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    14161 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    14221 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    14281 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    14341 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    14401 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    14461 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    14521 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggatcc
    14581 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    14641 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    14701 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    14761 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    14821 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    14881 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    14941 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    15001 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    15061 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    15121 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    15181 cagcgtttgc gcgacgcgtt tcagatcgtg gaacagatct ttacccacct gcagtaacgg
    15241 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    15301 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    15361 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    15421 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    15481 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    15541 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    15601 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    15661 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    15721 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    15781 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    15841 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    15901 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    15961 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    16021 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    16081 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    16141 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    16201 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    16261 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    16321 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    16381 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    16441 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    16501 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    16561 atagcgtaac gctttaccaa ggcacaaaaa aaagattact ggcccccagg agatgcgcat
    16621 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    16681 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    16741 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    16801 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    16861 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    16921 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    16981 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg gtgcacgccc
    17041 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatca
    17101 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa tgcctctgcg
    17161 atggcgcttt cactccccgt tcctacagcc attggacgac gaccatgcca acttttcacc
    17221 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    17281 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    17341 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    17401 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    17461 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    17521 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    17581 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    17641 tttggagaa
//
LOCUS       DADLXX010000080        17486 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLXX010000080 DADLXX010000000
VERSION     DADLXX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.080"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNX27_002602"
     CDS             complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNX27_002602"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7883768.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNX27_002603"
     CDS             complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNX27_002603"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7883769.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNX27_002604"
     CDS             complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNX27_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7883770.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNX27_002605"
     CDS             complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNX27_002605"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7883771.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5463..5660)
                     /locus_tag="JNX27_002606"
     CDS             complement(5463..5660)
                     /locus_tag="JNX27_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7883772.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNX27_002607"
     CDS             complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNX27_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7883773.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNX27_002608"
     CDS             complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNX27_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7883774.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNX27_002609"
     CDS             complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNX27_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7883775.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNX27_002610"
     CDS             complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNX27_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7883776.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNX27_002611"
     CDS             complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNX27_002611"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7883777.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQVVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNX27_002612"
     CDS             complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNX27_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7883778.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNX27_002613"
     CDS             complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNX27_002613"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7883779.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNX27_002614"
     CDS             complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNX27_002614"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7883780.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNX27_002615"
     CDS             complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNX27_002615"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7883781.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15979..17118
                     /gene="queG"
                     /locus_tag="JNX27_002616"
     CDS             15979..17118
                     /gene="queG"
                     /locus_tag="JNX27_002616"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7883782.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17101..17154)
                     /locus_tag="JNX27_002617"
     CDS             complement(17101..17154)
                     /locus_tag="JNX27_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883783.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17387..17462)
                     /locus_tag="JNX27_002618"
     tRNA            complement(17387..17462)
                     /locus_tag="JNX27_002618"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17427..17429),aa:Gly,seq:gcc)
BASE COUNT         3959 a         4985 c         4400 g         4142 t
ORIGIN      
        1 accatccaaa tctggatggc ttttcataat tcagaggaac tagctgcgct gacgaaccgc
       61 ttcaaataag caaattccgg ttgcaaccga aacgttcagg gaagaaacac ttcctgccat
      121 tgggatgctg atcaactcat cgcaatgttc acgggtcagg cgacgcatac cttcaccttc
      181 cgcgcccatc accagcgcca ggcgtccggt cattttgctc tgatagagtg tatgatccgc
      241 ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc aacatacgca tggtgcgcgc
      301 aaggttagtc acccgaatca gcggaacgct ttctgccgcg ccgcaggcta cttttttcgc
      361 cgtagcgttg agctgtgcgg agcgatcttt cggcacaatc accgcatgaa cgcctgcggc
      421 gtccgcacta cgcaggcacg cgccgaggtt gtgcggatcg gttacgccgt cgaggatcag
      481 caggaacggt tgatcgagcg aagcgatcag atccggcaga tcgttttcct gatactgacg
      541 tcctggcttc acgcgggcga taatgccctg atgcacggca ccgtcgcttt tctcgtcgag
      601 atattggcgg tttgccaact ggataaccac gccctgagac tcaagggcgt gaatcagcgg
      661 taacagacgt ttatcttcac ggccttttaa aataaagact tcctgaaaac gttcaggggc
      721 gcgctccagc agggcctgca ctgcgtggat gccgtaaatc atttcgctca ttaatgtact
      781 cgttgtttac gttttgcctg atgcactacg tttatccggc ctacatgatc tctgcaatat
      841 attgaatttg cgtgattatg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
      901 aagcgcattt tgtcagcaat ctaaccctct tcttttaaag agggtattga tcactctgcc
      961 acttttttct tcgccgcacg cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc
     1021 gatggctttt tcgcttttct cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca
     1081 ccgcggaagg cgctgtctgg ctcaaagttt acctttttac cgacctgacg acgcttgccg
     1141 ccttttttgc ctgcatcgcc ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc
     1201 ggtgcgcgtt cactggagat cagactaaag tcgattttgc gctcgtccat attaaccgct
     1261 tcgacgcgaa cttccacgcg atcgcccagg cgataagtct ggccgctgga ttcccccatc
     1321 aggcgttgcc ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc
     1381 agaccatcaa tgaacaagtc gtccagacgg acgaagaagc caaagccagt gacgctggaa
     1441 attacgcctt taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca
     1501 gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt
     1561 tgcaacatct cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc
     1621 ccctgctctt tcgccagcag atatttaatg gcgcggtgca gcgtcaggtc aggataacga
     1681 cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca gaccgaagtg accacggttt
     1741 tctggatcgt aaatcgcctg tttcatcgag cgtagcagca tggtttgcag catttctgca
     1801 tcaggacggt cggcaaccga ctccagcagt tccgcgtagt cacgcggttc cggcttgtta
     1861 ccacccggca gctccagccc cagctccgcc agcactgaac ggaaagaggt aatcgcttcg
     1921 gtgctcggct tgtcgtgaat acggaacagt gccggttctt tcgctttctc aacgaaacgc
     1981 gccgccgaga tattcgccag aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc
     2041 tgggtctgtt cgatacgttc aatacggcgt tcagcgttga aaatgaactt cgcttcttcg
     2101 ctctcaaatg agatcccacc gcgttcttca cgggctttat ccagcacttt atagaggtta
     2161 tgcaactctt cgagatgctt aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc
     2221 tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg
     2281 tagaatttgt agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac
     2341 aggcggtcta cctgcgggtt gagcgaacac aggccgttag agagcacttc cggcagcatc
     2401 gggataacct gcgaagggaa gtacaccgac gtgccacggt tacgcgcttc tctgtccagc
     2461 ggcgttggcg gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg
     2521 ccgccgcgtt ttttctcgca gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca
     2581 atggtgacca gcggtaaatc gcgcaaatcg acacggcccg cttttgcttc ttccggcact
     2641 tcttctttca gccccgcaac ctgttgctca acagcctgcg gccagatata cggaatttca
     2701 tgggtacgca gagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg
     2761 acgattttac ccaccgcttt ggtgcggcga gtcggacgct gagtcagttc gactacgacc
     2821 acaaagccca tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga
     2881 cggctatcgt caggaaccac aaagccgacg cccgcttcgg taaagtagcg accaacaatc
     2941 tggctggttt ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca
     3001 gcacccagcg gctgcgccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg
     3061 gagagataca aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtgg
     3121 ccaataacgg tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga
     3181 gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc
     3241 tgctcttcgc cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca
     3301 cgtttggtta aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca
     3361 gcttcgcgtt cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg
     3421 aagattttcg tcactccaac agcaataatt tataaagcgg ttgattctct tcaaccaaat
     3481 cggcaagcgt gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct
     3541 gtttcaaccg acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata
     3601 agggctccag ctcgcgcacc acatcaccaa tacgtatcgc actcgccggt ttacccaggc
     3661 gaatgccgcc attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga
     3721 ttattttgac catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg
     3781 tcatccgccc ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga
     3841 aactcgttaa ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga
     3901 tgataaacca gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg
     3961 aggatttaac actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact
     4021 gttagcccgg caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacgggtcgc
     4081 gcagaatcat ggtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccgg
     4141 tcagctcttc aatacgcttg atgtagttca gcgccgcctg cggcaggccg ctacgatctt
     4201 tcacgccgaa agtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt
     4261 tccagtcgtc agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag
     4321 ccacgcagag tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc
     4381 cagacaggga gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac
     4441 gacgacgacg acccgtagtt gcaccgaatt cgttaccctg cttgcagagg aactcgccag
     4501 tttcatcaaa cagttcggcc gggaaaggac ctgcacctac acgagttgag taagctttga
     4561 ggatacccag aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac
     4621 cagcagtggt gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg
     4681 taccctgcgc accttcgaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt
     4741 cagaaacgtc aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa
     4801 ctttctggta atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt
     4861 ccatcacttc tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca
     4921 gaccgcgacg tgccacttta tcttcataag caggcccgat accacgaccg gtggtgccga
     4981 tcgctttcgc gccacgcgct ttctcacgcg cgttatccag cgcaacgtga taatcaagga
     5041 tcagcggaca tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca
     5101 gttctttcat ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc
     5161 tggttacatt ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt
     5221 tgattacgag agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc
     5281 gttcagtcag aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta
     5341 cgacgacgtt gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc
     5401 gctttttcag agttacagca ctttttgcac tcaaaaatga cctcgaacag tctgtttttt
     5461 gttcagccaa tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca
     5521 ccgccaaaac gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc
     5581 catgccttcg ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc
     5641 agccagattg tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg
     5701 aggatgcggt ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc
     5761 ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg
     5821 ccagagaagc tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct
     5881 ttactgaatg catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcataatg
     5941 cggccctgac gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc
     6001 agtttttccg cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg
     6061 gcgcgcatac ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata
     6121 cgcacatcga caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact
     6181 ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atctgctgcc
     6241 ggggtagtaa cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg
     6301 agggtcagac gaccacggga atcggtgacg atatctttca cgtccaggcg accaatttca
     6361 gaacgcagac ggtcagagaa cttacgtttc aacagcactt ccgcttgcga aatgtcgcca
     6421 ccacccgttg ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagagtcg
     6481 acgatcaggt ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga
     6541 atacgtgcgt cgagcatttt caccgtttca atgaacggta tcttgaaatg cagaccaggc
     6601 tcataaacca aaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata
     6661 ccgcgctcac cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg
     6721 ataatcgcga taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct
     6781 gcgcgttggc gcggcgttgg tccataatat cgccctgact ggtggacgac gtgttgcttg
     6841 ctccactggt tgtggaggaa gaggctggcg gcagacgcag cagattgctg gcaccgttat
     6901 cgctcttcgc cgcaggggcg ttaccacctt tcaacatctg gtctaacggc agaaccatca
     6961 ggttgccacc tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg
     7021 tctcgatata cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt
     7081 tagcaaagcg cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg
     7141 cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg
     7201 cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg
     7261 ccgcttttac ttcttccggc ggacgagcag cctggaagtt gacgtccagc agcgtgatac
     7321 ccatgtcata cggacgaatc gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg
     7381 tacgaccttc cgtcagaatg cggtccatgg tgtatttacc gataactcca cgcagggcgc
     7441 tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg
     7501 gattggtgac gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca
     7561 gcatcacacc agaagcggcc agttcacgca cggcttccac gttcaccggt ttgacttcgt
     7621 cgataaacgt cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc
     7681 gtgttaccac gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa
     7741 tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt
     7801 gcgatgaact gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt
     7861 tgctcagttt gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt
     7921 ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc
     7981 cgttattacc gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc
     8041 ctgttgtctt tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc
     8101 aggtaatcga tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata
     8161 cgaacttgca gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc
     8221 tgataaaaac gactcctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc
     8281 acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc
     8341 tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg
     8401 aaatcttcca gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg
     8461 atctcttcaa gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca
     8521 tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgct
     8581 gccaccagat cgtgcggcag gtggcgaata aaccctacgg tatctgcaag tacggtttca
     8641 ccgacatctg caacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct
     8701 gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta
     8761 tatcccacca gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca
     8821 cgctgctttt caactctttc caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa
     8881 cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt
     8941 ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc
     9001 tcaacctgca acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg
     9061 ccggtgcggt cgataacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg
     9121 ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt
     9181 tcaactgctt taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta
     9241 atcacctgca atgcttcgac accggcggaa gagaccagag attcaaactc ctggaggtct
     9301 tccatatctt tgtcttgcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca
     9361 taacggtcaa acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg
     9421 ctccccgtgt aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
     9481 gaagtattct gtgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
     9541 ttactgtgat gagaaaccgg gcgagatggg acaacagtag aaatcgcgtg cttgtaaacc
     9601 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
     9661 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
     9721 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
     9781 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
     9841 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
     9901 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
     9961 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc
    10021 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
    10081 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
    10141 aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
    10201 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
    10261 cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
    10321 tgctgccgca accggatcta ccacctgaag ttgacgatgc aatgactccc aaccttgctc
    10381 tgccgcctgt tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa
    10441 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
    10501 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
    10561 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
    10621 gttcggcttc gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat
    10681 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
    10741 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
    10801 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
    10861 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
    10921 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
    10981 atacggactg cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt
    11041 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
    11101 tacccaattc cgctagggca gactgcgctt tttctaatgc cgatttttct tcgccagaaa
    11161 cttttagccg caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca
    11221 attgtgcctg acgcagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat
    11281 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    11341 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    11401 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat
    11461 tcggccaggg ggcagccgga cgactgcctg aggcgggcgc aggagagtag cgcggagcta
    11521 ccggctcacg agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg
    11581 ggatggcacg cggtgcgggt tgtggttcat cgtccagcgg tagcggcgtt tccagttgct
    11641 gttgtagcac gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga
    11701 aacgcacttc gtgtttggcg gggtgtacgt tgacgtccac ctgatgcggg tcgatctcca
    11761 gatacaacac aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga
    11821 tcgcatgatt gatcaggcga tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt
    11881 ccgccagtgc gggcgtagtg tgatttggat cggccaccca gccgcgcagg gtgagatcgc
    11941 cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta
    12001 agcgccgttc tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt
    12061 tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatttcat
    12121 caatatggct aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    12181 agaacagatc cagcacctcc agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca
    12241 cgtccatgtc gcgtccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    12301 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    12361 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgagcca
    12421 gcgccagcgc cagctcatct tttttgatac cgcagccgtt atcacgaatg cggataagtt
    12481 tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt
    12541 ccactagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    12601 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    12661 gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc accctgcggc gcagattgca
    12721 tcggatgcgc aaggaagtaa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    12781 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    12841 ccagtactga tgggatatcc ggcgagcgca gaacgccaag gctggcgtgt tctggtcgac
    12901 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    12961 ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    13021 actgactgtt cgccagcaca tcacccgccc cacccagcag ctccgactgt ttctcgtgct
    13081 gctccagcca gctggccatt tcactgttgg cgcgacggtt agagagcacc aatacggaag
    13141 cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    13201 tttgcttatg tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    13261 ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    13321 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    13381 cgtgtccggc atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg
    13441 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    13501 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt
    13561 tcgcaaccac gggcggcgga ggaggcggtg gcggcacatc ggcgttaatc gtaaagacga
    13621 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat
    13681 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc agagcgaatc gccttcacca
    13741 gattattgcc gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga
    13801 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    13861 acgttatccg tgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac
    13921 ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca
    13981 tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg
    14041 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    14101 ccgggtcagg aagaacacct gtaccttgtt gtggccactc cacaaggcag atggcatcgt
    14161 tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca aggcggtaca
    14221 aatcaaagtg atagaccatt aagttgtcga gcgtataggg ttcgaccagc gtataagtgg
    14281 ggcttttgac attaccctga tgacccagag cctgtaaaaa gccccggcta aaggtggttt
    14341 tacctgcgcc taaatcgcca tacagataga ttacggttgc tccatcgcag gctttcgcta
    14401 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    14461 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    14521 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg
    14581 acacagcctg cacaggctgc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    14641 ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata
    14701 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    14761 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    14821 acggaacagc ctaacaaccg tgcggcctcg ctaggatgcg gcgtgatcac gcgattgtga
    14881 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    14941 cgaaaattct caactttttg cagggctttt ttcccccact cttgctggcc cagaccggga
    15001 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat agtcagttca
    15061 tgcaccatca attctggtcg tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt
    15121 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    15181 gccgtgccgt gatcgccacc gataatcacc agccgcccgt gatcgccttt atgcgaagtc
    15241 gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    15301 tcctgacctg ccagccagct atccagcccc agtgagtcaa aatgcagttg tcctgtaaca
    15361 tcccgcgctt ttccagtgag caagcctagt ttcagcgcaa taaaagtgat ggtgtgatcg
    15421 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    15481 accgccacaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    15541 cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg
    15601 ggccagacaa tattcgaagc atggatctcg ccacccgcgt ttaaccatgc ttcgcgtgcc
    15661 agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccaacaacgt gacctcaatg
    15721 ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    15781 cacagcacca gccagtggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggcc
    15841 tcgccagcgc gaagcatcag ctcatagagt gtgagcccca gcacatctgc cgcctcgcgt
    15901 tctccgcggc ggatatcgtc ggcgtaccag acggtgtgtg gtatacttac ggggtttttc
    15961 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    16021 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    16081 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    16141 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattacccgg tacgctgcgt
    16201 gtaatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    16261 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    16321 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    16381 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    16441 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta
    16501 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc
    16561 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    16621 gatgctcgcc gctgtatctc ttatctcacc atcgaacttg aaggggcgat cccggaagag
    16681 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    16741 tggaatcgct attcgcaact cactacagaa gacgatttca gcccgcgtaa gccgctacac
    16801 gcaccggaac tcattgagtt atttgcctgg agcgaagaga agtttttaaa agtcacggaa
    16861 ggatcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc cgtggcatta
    16921 ggcaatgccc cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac
    16981 ccacttctcg atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg
    17041 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg
    17101 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    17161 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cggaatcttg atttttaact
    17221 tatttatcaa taggttaaaa aataccattc accgtgcgaa gtttctttga aattaagatt
    17281 acagcctgag gctgtggata actctgttta caagagtttg tcagagacaa caaaaaccag
    17341 caataacgca tcgcgtcgct gtggatattt tattgagaga agaatttgga gcgggaaacg
    17401 agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc
    17461 gcttgggtgg tctgtgcctt acagca
//
LOCUS       DADLXX010000081        17461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLXX010000081 DADLXX010000000
VERSION     DADLXX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.081"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            564..1334
                     /locus_tag="JNX27_002619"
     CDS             564..1334
                     /locus_tag="JNX27_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7883784.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JNX27_002620"
     CDS             1325..1798
                     /locus_tag="JNX27_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7883785.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGTLSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JNX27_002621"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JNX27_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7883786.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JNX27_002622"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JNX27_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7883787.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JNX27_002623"
     CDS             3233..3727
                     /locus_tag="JNX27_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7883788.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JNX27_002624"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JNX27_002624"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7883789.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JNX27_002625"
     CDS             complement(6410..7432)
                     /locus_tag="JNX27_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY7883790.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8484
                     /locus_tag="JNX27_002626"
                     /pseudo
     CDS             7571..8484
                     /locus_tag="JNX27_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8699..10060
                     /locus_tag="JNX27_002627"
     CDS             8699..10060
                     /locus_tag="JNX27_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883791.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNX27_002628"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNX27_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7883792.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNX27_002629"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNX27_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7883793.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="JNX27_002630"
     CDS             12610..13899
                     /locus_tag="JNX27_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7883794.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="JNX27_002631"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="JNX27_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7883795.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="JNX27_002632"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="JNX27_002632"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7883796.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="JNX27_002633"
     CDS             16225..16605
                     /locus_tag="JNX27_002633"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7883797.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17461
                     /gene="hpaH"
                     /locus_tag="JNX27_002634"
     CDS             16673..>17461
                     /gene="hpaH"
                     /locus_tag="JNX27_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7883798.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSIS"
BASE COUNT         4294 a         4627 c         4378 g         4162 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtacgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacgattt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg cttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccacaagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgaact gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
    17461 c
//
LOCUS       DADLXX010000082        17372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLXX010000082 DADLXX010000000
VERSION     DADLXX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.083"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..600)
                     /locus_tag="JNX27_002635"
     CDS             complement(<1..600)
                     /locus_tag="JNX27_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7883799.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNX27_002636"
     CDS             complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNX27_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY7883800.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNX27_002637"
     CDS             complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNX27_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY7883801.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNX27_002638"
     CDS             complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNX27_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7883802.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2535..4193
                     /gene="fliF"
                     /locus_tag="JNX27_002639"
                     /pseudo
     CDS             2535..4193
                     /gene="fliF"
                     /locus_tag="JNX27_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            4186..5181
                     /gene="fliG"
                     /locus_tag="JNX27_002640"
     CDS             4186..5181
                     /gene="fliG"
                     /locus_tag="JNX27_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY7883803.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5174..5860
                     /gene="fliH"
                     /locus_tag="JNX27_002641"
     CDS             5174..5860
                     /gene="fliH"
                     /locus_tag="JNX27_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY7883804.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQVHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5860..7233
                     /gene="fliI"
                     /locus_tag="JNX27_002642"
     CDS             5860..7233
                     /gene="fliI"
                     /locus_tag="JNX27_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY7883805.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7252..7695
                     /gene="fliJ"
                     /locus_tag="JNX27_002643"
     CDS             7252..7695
                     /gene="fliJ"
                     /locus_tag="JNX27_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY7883806.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7692..8819
                     /gene="fliK"
                     /locus_tag="JNX27_002644"
     CDS             7692..8819
                     /gene="fliK"
                     /locus_tag="JNX27_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY7883807.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8924..9388
                     /gene="fliL"
                     /locus_tag="JNX27_002645"
     CDS             8924..9388
                     /gene="fliL"
                     /locus_tag="JNX27_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY7883808.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9393..10397
                     /gene="fliM"
                     /locus_tag="JNX27_002646"
     CDS             9393..10397
                     /gene="fliM"
                     /locus_tag="JNX27_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY7883809.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10394..10807
                     /gene="fliN"
                     /locus_tag="JNX27_002647"
     CDS             10394..10807
                     /gene="fliN"
                     /locus_tag="JNX27_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY7883810.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYDVRITDIITPSERMRRLSR"
     gene            10810..11175
                     /gene="fliO"
                     /locus_tag="JNX27_002648"
     CDS             10810..11175
                     /gene="fliO"
                     /locus_tag="JNX27_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY7883811.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11175..11912
                     /gene="fliP"
                     /locus_tag="JNX27_002649"
     CDS             11175..11912
                     /gene="fliP"
                     /locus_tag="JNX27_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY7883812.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11922..12191
                     /gene="fliQ"
                     /locus_tag="JNX27_002650"
     CDS             11922..12191
                     /gene="fliQ"
                     /locus_tag="JNX27_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY7883813.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12200..12984
                     /gene="fliR"
                     /locus_tag="JNX27_002651"
                     /pseudo
     CDS             12200..12984
                     /gene="fliR"
                     /locus_tag="JNX27_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
     gene            13269..13892
                     /gene="rcsA"
                     /locus_tag="JNX27_002652"
     CDS             13269..13892
                     /gene="rcsA"
                     /locus_tag="JNX27_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7883814.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13936..14178)
                     /gene="dsrB"
                     /locus_tag="JNX27_002653"
     CDS             complement(13936..14178)
                     /gene="dsrB"
                     /locus_tag="JNX27_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7883815.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            14287..14514
                     /gene="yodD"
                     /locus_tag="JNX27_002654"
     CDS             14287..14514
                     /gene="yodD"
                     /locus_tag="JNX27_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY7883816.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14812..15627
                     /locus_tag="JNX27_002655"
                     /pseudo
     CDS             14812..15627
                     /locus_tag="JNX27_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="JNX27_002656"
     CDS             complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="JNX27_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY7883817.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGCIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         4372 a         4413 c         4470 g         4117 t
ORIGIN      
        1 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
       61 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
      121 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      181 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      241 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      301 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag
      361 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
      421 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
      481 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaacct tgtgaatctc
      541 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
      601 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
      661 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
      721 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
      781 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
      841 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
      901 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
      961 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     1021 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1081 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1141 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1201 tgtgccttga tctcattatg cataaaggca caatgatcgc gcaaatgcaa tcataattat
     1261 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     1321 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1381 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga
     1441 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     1501 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     1561 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     1621 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     1681 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctagtgtat
     1741 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     1801 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     1861 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     1921 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     1981 caagcatagt caacaggtta cacaacctac acctgcatag acatcacttc ctgatacgcc
     2041 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2101 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2161 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgatcg cggcgtgcag ctgcccggca
     2221 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2281 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2341 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2401 acgggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     2461 agtttgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     2521 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     2581 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     2641 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     2701 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     2761 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgc
     2821 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg
     2881 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
     2941 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     3001 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     3061 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt
     3121 ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc
     3181 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca
     3241 gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta
     3301 acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     3361 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
     3421 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     3481 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc
     3541 agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca
     3601 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     3661 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
     3721 tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg
     3781 aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg
     3841 gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc
     3901 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     3961 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     4021 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     4081 accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     4141 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     4201 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     4261 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     4321 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     4381 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     4441 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     4501 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     4561 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     4621 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     4681 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     4741 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     4801 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     4861 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt caatgatcgc
     4921 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     4981 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     5041 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     5101 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     5161 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     5221 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     5281 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggtccacg agcagggtta
     5341 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     5401 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     5461 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     5521 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     5581 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     5641 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     5701 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     5761 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     5821 gcaagaactc tgccgtctgg cagcactagg agtggtgtaa tgaccacgcg cctgactcga
     5881 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     5941 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     6001 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     6061 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     6121 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     6181 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     6241 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     6301 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     6361 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     6421 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
     6481 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
     6541 ggacgtgcac gctcagtggt gattgccgct ccggcggatg tttctccgct cctgcgaatg
     6601 caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     6661 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     6721 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     6781 ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     6841 gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc
     6901 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     6961 attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     7021 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     7081 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     7141 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     7201 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa
     7261 catggtgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgctcgc
     7321 ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag aacagctcaa aatgctgatc
     7381 gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc
     7441 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
     7501 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa
     7561 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
     7621 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
     7681 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat
     7741 tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat
     7801 tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtggca acagataagc
     7861 ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt
     7921 tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac
     7981 cgttaaccac cgctcagaca atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
     8041 acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga
     8101 tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg
     8161 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg
     8221 atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta
     8281 aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc
     8341 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt
     8401 ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac
     8461 aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag
     8521 tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc
     8581 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
     8641 aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
     8701 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
     8761 ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac
     8821 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
     8881 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
     8941 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
     9001 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
     9061 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt
     9121 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac
     9181 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
     9241 acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
     9301 taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
     9361 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
     9421 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
     9481 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
     9541 gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt
     9601 tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccatacc
     9661 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc
     9721 tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc
     9781 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg
     9841 aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga
     9901 aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca
     9961 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    10021 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
    10081 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    10141 tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc
    10201 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    10261 atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggcactctca
    10321 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    10381 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    10441 cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga
    10501 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    10561 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    10621 agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc
    10681 gctggatatt ctgaacaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga
    10741 taaatatgac gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag
    10801 ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca
    10861 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    10921 ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc
    10981 gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg
    11041 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    11101 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
    11161 ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta
    11221 tgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac
    11281 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    11341 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    11401 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    11461 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    11521 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11581 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11641 ccggcccgtt gcaggggcct gaagctgtac cgatgcgcat tttgctcccg gcctacgtga
    11701 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11761 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11821 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11881 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11941 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    12001 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    12061 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    12121 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    12181 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    12241 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    12301 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    12361 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    12421 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    12481 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttat
    12541 cgatcctggc gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta
    12601 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    12661 cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc acccaccaaa
    12721 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg
    12781 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    12841 gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg
    12901 ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt
    12961 attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc
    13021 cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa
    13081 gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta tacaacactt
    13141 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
    13201 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
    13261 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
    13321 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
    13381 accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat gaggactgtt
    13441 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
    13501 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
    13561 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg
    13621 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
    13681 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
    13741 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
    13801 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
    13861 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag
    13921 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
    13981 attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca ttgtgccttc
    14041 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
    14101 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
    14161 atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga ctgtctatac
    14221 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
    14281 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
    14341 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
    14401 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
    14461 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
    14521 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
    14581 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
    14641 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
    14701 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
    14761 ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca
    14821 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
    14881 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
    14941 attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta
    15001 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
    15061 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
    15121 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
    15181 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
    15241 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
    15301 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
    15361 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
    15421 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
    15481 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
    15541 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
    15601 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
    15661 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
    15721 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
    15781 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
    15841 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
    15901 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
    15961 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
    16021 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
    16081 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
    16141 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggaaaggagt
    16201 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
    16261 ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa atggtccaga
    16321 cgttcccagc taacatagcg actgtccata cgaatgcagc cccgcgtgtc atgttccatt
    16381 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
    16441 ttggaagagg tcaaaaatat cagcttactg tcatagagct gatactcacc atcgaggttt
    16501 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
    16561 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
    16621 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
    16681 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
    16741 cgcataaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
    16801 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
    16861 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
    16921 aatgcatccg atacgccatt gactggcaga gtgcgccgtc ggttgagttc gatttgccag
    16981 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
    17041 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
    17101 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
    17161 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
    17221 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
    17281 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
    17341 ttttatgatt ctggcataac ccggcaatag cg
//
LOCUS       DADLXX010000083        17341 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLXX010000083 DADLXX010000000
VERSION     DADLXX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.084"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNX27_002657"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNX27_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7883818.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="JNX27_002658"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="JNX27_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7883819.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="JNX27_002659"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="JNX27_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7883820.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="JNX27_002660"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="JNX27_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7883821.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="JNX27_002661"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="JNX27_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2739..3272)
                     /locus_tag="JNX27_002662"
     CDS             complement(2739..3272)
                     /locus_tag="JNX27_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7883822.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4494)
                     /gene="blaEC"
                     /locus_tag="JNX27_002663"
     CDS             complement(3361..4494)
                     /gene="blaEC"
                     /locus_tag="JNX27_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7883823.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNX27_002664"
     CDS             complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNX27_002664"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7883824.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNX27_002665"
     CDS             complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNX27_002665"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7883825.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5333..6067)
                     /locus_tag="JNX27_002666"
     CDS             complement(5333..6067)
                     /locus_tag="JNX27_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7883826.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNX27_002667"
     CDS             complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNX27_002667"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7883827.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8193..9170
                     /gene="epmA"
                     /locus_tag="JNX27_002668"
     CDS             8193..9170
                     /gene="epmA"
                     /locus_tag="JNX27_002668"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7883828.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9389..10891
                     /gene="yjeM"
                     /locus_tag="JNX27_002669"
     CDS             9389..10891
                     /gene="yjeM"
                     /locus_tag="JNX27_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY7883829.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNX27_002670"
     CDS             complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNX27_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7883830.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNX27_002671"
     CDS             complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNX27_002671"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7883831.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNX27_002672"
     CDS             complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNX27_002672"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7883832.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16587..17132
                     /gene="orn"
                     /locus_tag="JNX27_002673"
     CDS             16587..17132
                     /gene="orn"
                     /locus_tag="JNX27_002673"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7883833.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4002 a         4470 c         4547 g         4322 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtcctg
     2521 gattatctta gttattgctg gtctgccgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 actacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg ggtaaattgc tttgcaccca gcgggccata tcttcaatgg tcgacttcac
     3781 accataagct tcagcatcta acgcccctgg cgaaacatgc actgccttac cttcgcgata
     3841 tccccaggcg taattctttt cttctggggg aggtacatta atccacgtat ggttgagttt
     3901 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3961 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggagc
     4021 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4081 cggcacctgc aatggcaggc cgccagcggt gtaggtcgca agatgtaata gtgtgatccc
     4141 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4201 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4261 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4321 ggtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4381 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4441 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4501 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4561 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4621 catttgcccg caggcacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4681 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4741 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4801 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4861 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4921 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4981 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5041 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg tcagagtgat
     5101 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5161 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5221 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5281 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5341 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5401 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5461 atacaccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5521 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5581 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5641 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5701 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5761 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt catactgtcg gtgtaatcac
     5821 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5881 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     5941 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6001 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6061 cagccattcg ccttctcctt attattggct gcttccgcct tatcggctgc atccgcttcg
     6121 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6181 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgagaaagtt gacgtcgtca
     6241 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6301 gcggagtgcg ccatacattc agcaacgttc agaccgtggc tcagttcaat ggtgtagagc
     6361 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6421 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6481 ccttcttcca ttgccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6541 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6601 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6661 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6721 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6781 tcgataccgc ccatggtgta gtgtgcagtc ggacgtaccg gaatcggttc tttaaccgga
     6841 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6901 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatagtg
     6961 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7021 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7081 taacggtagc catttttgtt gaccagaata ccgccttcac cacggcagcc ttcggtcatc
     7141 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7201 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7261 ttgtaacgat aaacgcgacc cgcaccgccg gtagccataa cgaccgcgtt agcacggatc
     7321 tgcaccaacg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7381 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7441 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7501 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7561 gggcatcccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7621 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7681 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7741 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7801 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7861 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7921 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7981 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8041 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8101 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8161 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8221 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8281 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8341 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8401 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gctggttgtg
     8461 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8521 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8581 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8641 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8701 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8761 cgttgctaca attgctgttt acctttggcg tagaggcaaa tattggtaaa gaaaaaccga
     8821 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8881 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8941 atgaattaac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9001 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9061 gtatgcctga ctgctccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9121 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9181 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9241 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatcaagg tatcaattct
     9301 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9361 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9421 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9481 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9541 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9601 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9661 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9721 aacattcctc tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac
     9781 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9841 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9901 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9961 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10021 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10081 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10141 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10201 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10261 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10321 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10381 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10441 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10501 ggttactgtc ttcattctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10561 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10621 tttcccgttt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10681 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10741 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10801 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10861 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10921 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10981 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11041 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11101 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11161 cgtggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11221 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11281 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11341 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac actacgcgcg
    11401 tgaccgagtc agagagcgac cagttgataa actgcgcggt aataaacgcc ttgttcggca
    11461 cgattatctc tttacggtcc cagtcgctga tggtggtggc gcgggtgtta atttttgtca
    11521 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11581 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11641 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11701 cgaccagccc gccaatcagc atcagcagat attttgtgat ggtggtgatg gcgtaacccg
    11761 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11821 gcaccagctg cgtggtgatg ataaatacca gaatggcaat cagcaccgca ccgagggtaa
    11881 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11941 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa cgcagggcga
    12001 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12061 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
    12121 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12181 gcctgcgacg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12241 accagatggc aaccgaggtt tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac
    12301 ccaccgccga cgccagaatg gcaaccagtg gtgcgccaat catcatgttc cacagcatat
    12361 ggttggtaat gttgtcgccg ctgccttctt tgttgagata caacggaata ccggcttttt
    12421 tcaggcttag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12481 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc
    12541 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12601 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    12661 gcggcacggt ggccgttaca ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    12721 cgcgcaagcc gtaacccagc gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg
    12781 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    12841 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    12901 gaatcaacgc gccaaacagc ggcaaaattg tctctttgct ggtcagcatc atcacactgg
    12961 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13021 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13081 ttgcttcgtt cacctctttc agcgcatcct ccagttgccc gttggagact ttcagcttgg
    13141 tcagttccaa tagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13201 tgcgcagttg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13261 gatggatttg ccgtagcagc ggctgtttat taagtaaatc ctcataacgt aaccgttgca
    13321 cacgcaactg cgccatttcg gtatcaagct gttgtggttt tggcatttct ggtagccgtg
    13381 ccacctgcgc ccgcagcgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    13441 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13501 gcgaggcaac gagctccatc cgctgcgcct gttgattcag cgctgccgat agttcgcggt
    13561 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagtaatt
    13621 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctattcaac tgattacgta
    13681 agccctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    13741 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    13801 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    13861 tgccggtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    13921 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    13981 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14041 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14101 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14161 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14221 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    14281 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14341 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14401 ccaatggcct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    14461 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14521 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    14581 tggaaccgag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14641 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    14701 ccgcccagac cgtctcaatg ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg
    14761 ggccaaattc ggtatcgaaa aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    14821 cgttctgagc ggtgagatgg ttaacggaaa agagatcgcc cggcacgtag atcatctcac
    14881 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    14941 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15001 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15061 ggctgataac gccatcggca ggcatgacca gtacattcgg atcggtatcg attgggcgta
    15121 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15181 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15241 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15301 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15361 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    15421 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    15481 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15541 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15601 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15661 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    15721 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    15781 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttaccga
    15841 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    15901 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatgttg cggtagatat
    15961 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16021 cgataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16081 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggctatgg
    16141 gtttcacgcc gtcgtagaaa tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt
    16201 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16261 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16321 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16381 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16441 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    16501 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16561 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16621 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16681 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16741 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    16801 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    16861 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggacagg
    16921 accgtcgttt cctgtttaaa tacatgccgg agctggaagt ctacttccac taccgttatc
    16981 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17041 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17101 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17161 atccacttgc ggcggcgtga ttttgacgct aaatcgaacg tcttgctgat aatttcagca
    17221 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    17281 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17341 a
//
LOCUS       DADLXX010000084        17238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLXX010000084 DADLXX010000000
VERSION     DADLXX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.085"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..170
                     /locus_tag="JNX27_002674"
     CDS             <1..170
                     /locus_tag="JNX27_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7883834.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..446)
                     /locus_tag="JNX27_002675"
     CDS             complement(171..446)
                     /locus_tag="JNX27_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7883835.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="JNX27_002676"
     CDS             complement(467..703)
                     /locus_tag="JNX27_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7883836.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNX27_002677"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNX27_002677"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY7883837.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPVPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNX27_002678"
                     /pseudo
     CDS             complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNX27_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNX27_002679"
                     /pseudo
     CDS             complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNX27_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6356..6853
                     /locus_tag="JNX27_002680"
     CDS             6356..6853
                     /locus_tag="JNX27_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7883838.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6950..7648
                     /gene="proQ"
                     /locus_tag="JNX27_002681"
     CDS             6950..7648
                     /gene="proQ"
                     /locus_tag="JNX27_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7883839.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7668..9716
                     /gene="prc"
                     /locus_tag="JNX27_002682"
     CDS             7668..9716
                     /gene="prc"
                     /locus_tag="JNX27_002682"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7883840.1"
                     /translation="MSMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9908..10789
                     /gene="htpX"
                     /locus_tag="JNX27_002683"
     CDS             9908..10789
                     /gene="htpX"
                     /locus_tag="JNX27_002683"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7883841.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10835..12208)
                     /locus_tag="JNX27_002684"
     CDS             complement(10835..12208)
                     /locus_tag="JNX27_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883842.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12385..13176
                     /gene="kdgR"
                     /locus_tag="JNX27_002685"
     CDS             12385..13176
                     /gene="kdgR"
                     /locus_tag="JNX27_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7883843.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13319..13558)
                     /locus_tag="JNX27_002686"
     CDS             complement(13319..13558)
                     /locus_tag="JNX27_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883844.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13717..13860
                     /gene="mgrB"
                     /locus_tag="JNX27_002687"
     CDS             13717..13860
                     /gene="mgrB"
                     /locus_tag="JNX27_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7883845.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13935..14222
                     /locus_tag="JNX27_002688"
     CDS             13935..14222
                     /locus_tag="JNX27_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883846.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14892..15035
                     /locus_tag="JNX27_002689"
     CDS             14892..15035
                     /locus_tag="JNX27_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7883847.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15048..15257
                     /gene="cspE"
                     /locus_tag="JNX27_002690"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15048..15257
                     /gene="cspE"
                     /locus_tag="JNX27_002690"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7883848.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15423..16232
                     /gene="rlmA"
                     /locus_tag="JNX27_002691"
     CDS             15423..16232
                     /gene="rlmA"
                     /locus_tag="JNX27_002691"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7883849.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNX27_002692"
     CDS             complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNX27_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7883850.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4554 a         4170 c         4346 g         4168 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacctg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tgggacgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccggcttccg gcgcttcaac aataatgctt aaaccatcta agtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cctagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gttttattat tactccacgc
     4141 tggctgtggg ccagatcttc atacagacca aaggtatctt ccgcctcggc aggtttcgac
     4201 tcttcaggtg aatcgaaggc aatcgcaccg ttaaccagtg ccgccagact ttccagtttc
     4261 accttcgcgc cactgatact gacgttggca tcaacgccgg aaacgttcca gaaacggcta
     4321 ccttttttca ccagatcggt aaaacgccgc tcgatcagga catcaatcac cacgccttgc
     4381 ttgttgggat tgatggcata gtcgtagact tttcccaccg ggatcttgcg gaaatagacc
     4441 aatgaaccgc tgttcagcga accgagatcg ggggcttgca ggtggatcat cagatcgcca
     4501 ttgtccagcc gatatttcgg ttgggtatcg agcgcgacaa agtgatcctg ctctttacct
     4561 ttacccggca tcatgccgat atagttccca ccgacgaggg cgtccagccc ggagacacct
     4621 gccaacgatg cttttggcgt catcagccag aactgagtct cttcgcgcag cgcatctttc
     4681 atatcggact tgatgctgac cttgacttca atcttacgaa gatcgtcgct gaggctgata
     4741 tcctgcactg ttccgacttc aacgccctga taacgaacag gcgtacggcc cggaacaata
     4801 ccatccgccg acataaagtc gatggtgacg gtattacccc ggtcctgata actgtcccaa
     4861 atcagccaac cggcaatcat tagcgcgatg aaaggcagca gccagaaagg tgagatacgg
     4921 cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg tttcctgact catgtgcatc
     4981 ccaaagtaag cggctgtcca gccattccac agcaagaata gtcaaaatta cggcagcgcc
     5041 aaaataaaac gcagccggtc ccatagtaaa agcgaggatc tgatcgcgat taatcagcga
     5101 catggttaaa gatatgacaa acaggtcgag catcgaccag cgtccaatcc aggtcaccat
     5161 ccgcagtaac agaatgcgtg tgcgtaaacc ttgctggcat ttaaaatgaa tgctcaacag
     5221 taaagtgaac atgacgatca ctttagtaaa cggtaccaga atactggcga taaacacgat
     5281 tcccgcaacc gcaatgttgc tacttgccag cgacataatt ccggaaagaa tcgtatcttc
     5341 ctgccggcca ccattcagat aaatgataga aataggcaat aggttggctg gtaacaacaa
     5401 aacgattgac gctaacagcg ccgcccagca tttttgcaaa ctatgacgac gacgcaggcg
     5461 tagcgggata tggcaacgcg ggcagagacc acgctgatca ggatagccgg taaaatggca
     5521 cccaagacag acacgaagtt tctcgtccct acgcgtagcg gggcgctgcg gataatatcg
     5581 ctcccacagc tcctcaacat taagatgtga caacgtcacc gtcgttaaaa tcaccaacgc
     5641 cacaaaagag aacaagccga cacccgcctg gatatgggca taatcctgta ccttatatag
     5701 aagcaacgcc aatgccgacc aggtagatat ccagcattac ccactcttta agtcgctcaa
     5761 gcatcagcag taccggacgt aaattcattc ccagtcggtt accaaaccat aaatatgcta
     5821 tggaggacac cagaatgagg ggggcaccaa taacgcagaa aaagaccatt gatcccgtta
     5881 tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat gacgttggcg tcaatacgaa
     5941 tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcata agcaacagca
     6001 tggtgaaggc cattgccgcc aggcgcgtta gcgacaagtc gcgcccgtcg cgaatttttg
     6061 cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg ctaaacagca
     6121 tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg attgccctca
     6181 ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg tgccaaatgt
     6241 aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat taacgcattt
     6301 aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt aaataatgaa
     6361 caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg cgggagaaac
     6421 cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tacgagcgtc tcactgacgt
     6481 taactgggca ggtttttatt tgcttgagga cgatacactg gtactcggac catttcaggg
     6541 caaaattgcc tgtgtccgga tacccgtcgg gcgcggggtg tgcggcacag cggttgcccg
     6601 caatcaagtg cagcgtatcg aggatgttca cgcgtttgac gggcatattg cctgtgatgc
     6661 ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta ttggtgttct
     6721 cgacatcgat agtaccgtct tcgctcgctt tacagacgag gacgagcaag gcttacgtca
     6781 gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat tctttgcgag
     6841 cgtcgcagga taatcaacgg ataacgtagc atttactggt agcgtcatta taatgacgcc
     6901 tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca tggaaaatca
     6961 acctaagttg aatagcagta aagaagtaat cgcgtttctc gccgaacgtt ttccccactg
     7021 tttcagtgcg gaaggtgaag cgcgtccgct gaaaattggt atttttcagg atttggtcga
     7081 tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt tacgtctcta
     7141 cacttcgagc tggcgttatc tttacggtgt taaacccggc gcaacgcgtg tcgatcttga
     7201 cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca agcagcttga
     7261 agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa aacgcgaagc
     7321 tgccgcagct gctggtgaga aagaagacgc accgcgccgc gaacgcaagc cacgtccgac
     7381 cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc cggtagagaa
     7441 agcgccaaaa acagtaaaag cacctcgcga agaacagcac accccggttt ctgacatttc
     7501 agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga tggatgccac
     7561 cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta tgtctttgat
     7621 tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg agcatgtttt
     7681 ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc gctgtagaag
     7741 atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat gcgacggtaa
     7801 gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac ctcgatcagg
     7861 cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc cacaatgtgc
     7921 tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc gatgaactgc
     7981 gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag cgccgttttg
     8041 aacgttacca gtacgctttg tcggtactgg aaaagccgat ggatttcacc ggcaacgaca
     8101 cttataacct tgagcgcagc aaagcgccct ggccgaaaaa cgaggctgag ttgaacgcgc
     8161 agtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctgacagga aaaacggata
     8221 aagaaattcg tgaaaccctg acacgccgct acaaatttgc cattcgtcgt ctggcgcaaa
     8281 ccaacagcga agatgttttc tcgctggcaa tgatggcgtt tgcgcgtgaa atcgatccgc
     8341 ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg agtttgtcac
     8401 tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc aattcgatgg
     8461 tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa attgtcggtg
     8521 ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat gatgtggttg
     8581 ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct gctggtaaag
     8641 ggaccaagac ccgtactgta acgttgaccc gtgaacgtat tcgtctcgaa gaccgcgcgg
     8701 ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat attccgggct
     8761 tctatgtggg tttgacagac gatgtcaaag ttcaactgca gaaactggaa aaacagaatg
     8821 tcagcagtgt gatcatcgac ctgcgtagca atggcggtgg ggcgttgacc gaagcggtat
     8881 cgctctcagg tctgtttatt cctgcgggtc ccattgttca ggtccgcgat aacaacggca
     8941 aggttcgtga agacagcgat accgacgggc aggtgttcta taaaggccca ctggtggtac
     9001 tggttgaccg tttcagtgct tcggcttcag aaatctttgc cgcggcaatg caggattacg
     9061 gtcgtgcgct ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag caataccgtt
     9121 cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg ggttctgtgc
     9181 agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt aaaggcgtaa
     9241 caccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag aaattcgaag
     9301 ataacgcgct gccgtgggat agcatcgatg ccgcgactta tgtgaaatca ggagatttaa
     9361 ctgcctttga accggagctg ctgaaggaac ataatgcgcg tatcgcgaaa gatcctgagt
     9421 tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag cgcaatatcg
     9481 tttctctgaa ctacgctgtg cgtgagaaag agaataatga agatgatgcg acgcgtctgg
     9541 cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaag ctggatgatc
     9601 taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat atcgcactcg
     9661 atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgctcccgtc aagtaatatc
     9721 aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg taaatcagat
     9781 gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga cttgaaaata
     9841 gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact gaggtaaaaa
     9901 gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa tggtcgtttt
     9961 cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc tgatgatcat
    10021 ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca aatggatggc
    10081 attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg aacgttggct
    10141 ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc aagtggctat
    10201 ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg cctctctggt
    10261 tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg cggtaattgc
    10321 tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga ttcagggcgt
    10381 ggtgaacacc ttcgttatct ttatttcccg tattctggcg cagcttgccg cgggttttat
    10441 gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct actttgcggt
    10501 tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca tgtggttctc
    10561 gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc gcgagaaaat
    10621 gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa ccagcatgat
    10681 ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgttcatga ctcacccgcc
    10741 gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat atgaagtaaa
    10801 tactaaaaag cgcgtcctga tcgacgcgct ttttttatgc cctggctcgt ggctgagtga
    10861 tacgtaaacc actgacacag gcagcaatca ctgccagaat agccgcagcc atcagcgaga
    10921 cgtgcgtacc attatcacca aactgattta gcatcagcgc caccagcgcc gcgccactac
    10981 tctgacccag tagacgagcc gttcctaaca tgccactggc cccaccgcta cgttcgcgcg
    11041 gagcggaggt aataatggtg tggttattgg gtgactggaa taagccaaat ccagcgccac
    11101 ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc agaaccaggg
    11161 aaaaaagccc cgcagccatg atgaacaacc ctaaagcacc cagcaatcct gcatgtacgc
    11221 gttcaatcaa atagcctgcc agcggagcca tcaccatcgt tgctaacggc cacggtgtca
    11281 gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa aagggcaggg
    11341 aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata gaaagtgaaa
    11401 acagcgggat acgcagtaaa tccaccggta gcagcggtac gggaagagaa agctggcggc
    11461 gaataaagaa aataccaaca acaaccatta ccaccagttc cgcagcaatt aacgtcagcg
    11521 attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt aacgcgttca
    11581 tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg ggtggcagaa
    11641 aacgcatcgc cagaagcagg gcgataatac ctaacggtac gttgattaaa aataaccatt
    11701 tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca gaagagacgg
    11761 caacaataaa cgagtttatg cccatccctc tccccagaaa acgttgtgga tagatcaggc
    11821 ggataagtgc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt atgacacgcg
    11881 caagggtgag catttgcagc gaatcagaaa gggcgcagaa cagtgaagac aacagaaaaa
    11941 cgaccagacc gcatttataa atacgtcgat agccaaacat atcgcccaga aacgaaaacg
    12001 agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg gaactggctg
    12061 gtgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    12121 ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    12181 gcaggccgtc ggcctgaact tttggcatag gaattttata tctttggtga ataatcaaca
    12241 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    12301 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    12361 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    12421 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    12481 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    12541 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    12601 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    12661 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    12721 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    12781 tattcacgga ttgggcgtcg taatccgctg tacagcaccg cgattggtaa ggtactgctg
    12841 gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    12901 accgagcgga ccatcaccag tacagaagcg ttattacccg ttctggacca ggtgcgcgag
    12961 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggtaccg
    13021 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    13081 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    13141 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    13201 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg cgttatatta
    13261 aacgcgcttg ctataagaat attttactca tgagtgagaa tctggttatt tattaccctt
    13321 aaccattatc gaccacgata ttgcttttac gtaacagtgg gcaatctgtt atccccaaga
    13381 aaccactttt agtgtgcaag tattgtaccg tgctggtgcc tctggcagtc agataggtac
    13441 attgcaaacc taatcctgcg gcattctctt tgcttccaat caaaacgcca tatccgctga
    13501 gtaataatcc tatccatacc agtgctatca gcataactgt gcgaatgatg aatcgcatta
    13561 caatctcttc tctttttatg ttcgcttaat cgtagcggca atatgcgctg aagcaagcta
    13621 ctcattccga aaaagcacga atatcgacat agttaggcgc tgtttaacta acgcatgcta
    13681 gtttaatggc ataaggtagg tgaagcggag attggagtga aaaagtttcg atgggtcgtt
    13741 ctggttgtcg tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa catgatgtgc
    13801 gatcaggatg tacaattttt cagcggaatt tgtgccatta accagtttat cccgtggtga
    13861 tatcattttt tcagcactag attttcttcc tctccagcag tggtagaata gcgcctctac
    13921 tcagaggtgg tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt gcgtctttgg
    13981 ttgtatcggt ggtggttctt cttatcgggc tcatcttgtg gttttttatc aatcgtgcca
    14041 gttcacggac taacgaacag attgaactgc ttgaggcgtt gctggatcag caaaaacgtc
    14101 aaaatgcact gttacgtcgt ttgtgcgaag caaacgaacc agagaaagca gataaaaaga
    14161 ccattgagag tcaaaaatcg gttgatgacg aagatattat tcgcctggtc gccgaacgat
    14221 aatccttcct caatcttgag taaccccgtc gttgaagggt tactcaattc atcctgatta
    14281 tacgcactgt catggtgcaa ctgcttcaaa ttaaaactct ggtctgactt caaataatta
    14341 tcatattatt tatttgcgta acgggatatc agaaaaagat attttcctca ttaatttatt
    14401 aggatgttta catcagattt gtgattaagc gtggtattat ttattagcga aacgtttctc
    14461 tcttgatttt tttgctcact catcaatttt tcttatttta aatttacaat ccctttgggg
    14521 attgacttct ctttagggta attgatagcc gttaactgac tgttttatga gaaaaagtga
    14581 tataactttt tattcattgc atagcagaaa atgtgatatt gcacgcacta tgtaataact
    14641 tctccctctg gcctggaaca actgaactta ttgaactatg ttagaaacta cgccagttta
    14701 agtatctgcc tgaactggca aggttaagca caatgatata tcggcgcgta ttccgttgca
    14761 taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg cgtctgatcg agacatgttt
    14821 aaaaatggct tgccataatt aacgttgtat gtgataaaag atttcgggtt aaacgaggta
    14881 cagttctgtt tatgtgtggc attttcagta aagaagtcct gggtaaacac gttgacgttg
    14941 aataccgctt ctctgccgag ccttatattg gtgcctcatg cagtaatgtg tcagttttat
    15001 ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta
    15061 aaggtcaggt taagtggttc aacgagtcta aaggttttgg cttcattact ccggctgatg
    15121 gcagcaaaga tgtgttcgta cacttctccg ctatccaggg taatggcttc aaaactctgg
    15181 ctgaaggtca gaacgttgag ttcgaaattc aggacggcca gaaaggtccg gcagctgtta
    15241 acgtaacagc tatctgatcg aatccactga tctgaagtgt gaatacgctt caatctcgct
    15301 ataaagcctc gtcgaatgcg aggcttttta ctatgcttta tcttcgctcc tggcgttcgg
    15361 atatttgcca gccacgtgat tcgcgttaca cttgcggcct ttagtatcct gccggagttg
    15421 tcatgtcgtt ttcctgtcca ctttgccatc agcctctttc gcgtgaaaaa aacagctata
    15481 tctgtcccca acgacatcag tttgatatgg cgaaagaagg gtatgtcaat ctgctgcccg
    15541 ttcagcataa acggtctcgt gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg
    15601 cattcttaga tgccggacat tatcagccgc tgcgtgatgc aattgtcgcc caactgcggg
    15661 aacggcttga tgaaaaggcc acggcggtgc tggatattgg ctgtggtgaa gggtattaca
    15721 cacacgcatt tgccgatgcg ttgcccgaaa tcaccacgtt tggcctggat gtctcgaagg
    15781 tagcgataaa agcggcggcg aaacgctatc cgcaggtcac tctttgtgtc gcttccagcc
    15841 accgtttgcc gttttccgat accagtatgg acgccataat acgtatttac gcgccgtgta
    15901 aagcagaaga attagcacga gtagtgaagc ccggcggctg ggtcattact gccacgccgg
    15961 gaccgcgaca tttgatggag ctgaaggggc tgatttacaa tgaagtacat cttcatgcac
    16021 ctcatgcaga acaactggaa ggttttacat tacagcagag tgcggagttg tgttatccga
    16081 tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat gacgccgttt gcctggcgtg
    16141 cgaagccaga agtctggcaa acactggcag caaaagaagt gttcgactgc cagacggact
    16201 ttaatattca cctctggcag cgttcttatt aaccgtggaa gtgcgtccag aggatctgga
    16261 cgccgatgcc gatcagcacc agcccgccga gaatttccgc ttttttccca ataattgagc
    16321 cgataaagcg accaaccatc atccctaatg tcgacataat caaggttgca caaccaatgg
    16381 ccaatgcggt cgcgataatg ttgacctgaa ggaaagcaag accaacaccc acagccatgg
    16441 catccaggct ggtggcaatc gcggtggtta ccagtagcca gaaaccgtgc cgacggcgcg
    16501 gctcttcatc ttcatcatct gagccacgaa aaccctcaat aatcattcgc ccgccgagga
    16561 atatcagcag cacaaacgca atccagtgat tccattcaag gacaaaccgg ctggctaaca
    16621 tgcccattcc ccagccgatc agcggcgtca gggtttcgac ggcaccaaaa ataaggccgg
    16681 ttcgcaatgc ttcagaaaat ttcggtttat ggaggggggc acctttaccg attgatgcag
    16741 caaatgcatc catcgacata ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa
    16801 cgtcctgacc ggggtatcca taaaacacat catcgccccc agcaaacatg catacatgaa
    16861 caacaagccg cgatgggttt tcgcgttcat gcaggcatag cgattgatgt gcatatggtc
    16921 tcgcctgact gaaagtcttc agtccgtacg tgccacgttt tgcaacgagt atgttgacac
    16981 gtacatttcc ggaatctgga aatcggctac tccccaaatg agggcgcaac cttaacatat
    17041 tttgagaata taaaacaact gttggaatat attatttcat tgctttattg ataacgattt
    17101 tcatttatgt ttatatatca atgaaacgtt aaaataaata acaaataagg gaagagataa
    17161 tatagcctaa gctatgtctg attattaaaa atacactaaa catagctttg gctaaattca
    17221 taagttattg agttgata
//
LOCUS       DADLXX010000085        17183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLXX010000085 DADLXX010000000
VERSION     DADLXX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.086"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(329..673)
                     /locus_tag="JNX27_002693"
     CDS             complement(329..673)
                     /locus_tag="JNX27_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7883851.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JNX27_002694"
     CDS             805..2715
                     /locus_tag="JNX27_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7883852.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JNX27_002695"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JNX27_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7883853.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JNX27_002696"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JNX27_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7883854.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JNX27_002697"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JNX27_002697"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7883855.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JNX27_002698"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JNX27_002698"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7883856.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNX27_002699"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNX27_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7883857.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8853)
                     /locus_tag="JNX27_002700"
     CDS             complement(8251..8853)
                     /locus_tag="JNX27_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY7883858.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKQSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTNEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9210..9761)
                     /locus_tag="JNX27_002701"
     CDS             complement(9210..9761)
                     /locus_tag="JNX27_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7883859.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="JNX27_002702"
     CDS             9987..10214
                     /locus_tag="JNX27_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7883860.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
     gene            10208..10639
                     /locus_tag="JNX27_002703"
     CDS             10208..10639
                     /locus_tag="JNX27_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883861.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKVLEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNX27_002704"
     CDS             complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNX27_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY7883862.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNX27_002705"
     CDS             complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNX27_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY7883863.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNX27_002706"
     CDS             complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNX27_002706"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY7883864.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            12107..12361
                     /gene="yoaF"
                     /locus_tag="JNX27_002707"
     CDS             12107..12361
                     /gene="yoaF"
                     /locus_tag="JNX27_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7883865.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(12383..12730)
                     /locus_tag="JNX27_002708"
     CDS             complement(12383..12730)
                     /locus_tag="JNX27_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7883866.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNX27_002709"
                     /pseudo
     CDS             complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNX27_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            14063..14884
                     /locus_tag="JNX27_002710"
     CDS             14063..14884
                     /locus_tag="JNX27_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7883867.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(14841..15287)
                     /locus_tag="JNX27_002711"
     CDS             complement(14841..15287)
                     /locus_tag="JNX27_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY7883868.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            15457..15561
                     /locus_tag="JNX27_002712"
     CDS             15457..15561
                     /locus_tag="JNX27_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883869.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNX27_002713"
     CDS             complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNX27_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7883870.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16108..17166)
                     /locus_tag="JNX27_002714"
     CDS             complement(16108..17166)
                     /locus_tag="JNX27_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7883871.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQHVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
BASE COUNT         4406 a         4175 c         4358 g         4244 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgattagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag gtgagtattc gccgcaatct tgaaaagctt gcagccaatc atccagaact
    10321 acgtcgcaag cgtacgggtt caaaggtttt ggaatttcat atatcagttc taccagcggc
    10381 agcacgtgtt gagttgttat taaaatctga gcaaatagaa acaagccagg ggtattttga
    10441 aatcgcccgc cccacgctgg aagcgcacga ttacgatcgt gaggcactgt ggagcaaatg
    10501 ggataaagcc agcgattccc agcgcagact tgctgaaaaa tggttgcctg cggttcagac
    10561 tgcagacgaa atgcttcagc cgttattggt gcgattgtcg tgctatttat ctacaggaag
    10621 attaaaagtt aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt
    10681 agaggtgacg ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca
    10741 tcataataat cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa
    10801 aaaccatgct aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat
    10861 tcgcatgtta tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc
    10921 gcgacaaaag gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca
    10981 tgcagggcac tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca
    11041 ccgggataat acaaatcaac atcacctaag tgatattctc cacgtatttg ctgttgaata
    11101 cgttctcgca gcgacgaaat atccgcattc tcgttgttta gtgaaacaac caaaaactca
    11161 tcaccaccga gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca
    11221 acctgaatga gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag
    11281 ttatccagat cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac
    11341 agcgtcgtca gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta
    11401 tacgattgcg caatcagcgc agcattggct tcgtgcagct gttcaaccag tgcctctttt
    11461 tgaatatatt gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc
    11521 cactggcgct tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag
    11581 tgaattggcg tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcgccc
    11641 caacggtcag ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac
    11701 gagtaatccc acgagacggt aaaattctcc gggatgtgca ttttctggct gttacgggca
    11761 aacataatat gctgcaggcg cgcttcgaca tccactttgg tcaggtaggt tgattccata
    11821 tcagtgacca tttccagcat ctccagaagc tgtcggacca gactttccag tgactgttct
    11881 ttggcaaggg attgcgagac gcgggcgata atctgatctg acacggaaca actccaggat
    11941 acggcgggta agtgctgatt atttaatctt tgcaggcatg ataaaacatc atcaacaaag
    12001 atgaaacaag acgtaatatg ttttgagtga atgtaataaa aatagtgctg caaaacaaaa
    12061 cgataccgcg atactctaag cttggataaa tatgtgagat caataaatga aaattatcag
    12121 ttttgttctg ccttgcttgc tggtcctggc tggttgttca accccttctc agccagaagc
    12181 acctaaaccg ccgcagattg gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg
    12241 cgggacgctc attccagtgc agacagcgca aggggtcagc aacaattgca aattaccggg
    12301 cggtgaaacg attgatgaat gggcattgtg gcgacgggac catccggctg gtgaaaaata
    12361 accgccggat gccgtactaa aatcacaagc tacgtagcca gtcggccagc accagcgcat
    12421 ggttctgcgt ggtgtttttt gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt
    12481 ccgccagccg ctttccttct tgctcgtgtt gcgccagttc tgcgcgatat tgctcgcgaa
    12541 aggttgcaaa atcgacgact tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga
    12601 tttctttatc ccactcatca agggctaaat cggttttttt gataccgcgc ggccagaggc
    12661 ggtcgaccag tacgcgataa ccatcgctct gttcagccgg atcataaacg cgtttgcact
    12721 gaatattcat cgtgtttgtt ctccctttca cgcaagattt gtcacgtcac tatagcaccg
    12781 gatattagcg aatttctttg tctctcccgg cgcaaagtcc gaaaatcgcc atcagtagtg
    12841 aaagtattgc aacacccatc agtggtacgg accagttacc gttagcatcg tgtattttac
    12901 ccattagcgg cggcccacag gctgccagca aatacccgac ggattgtgcc atccccgaga
    12961 gtgccgccgc ctgatgcgca gaacttgccc gaagaccaat gaacgtcaac cccagtatca
    13021 ttgttgcgcc ggaaccaaaa ccgaaaagca gagtccaggt gatcgcgtgc gctggcataa
    13081 agcagagccc aaccgcgccc actgcgcaca tcaaggcaac gaacgctgca ataccacgct
    13141 ggtctttcac atgatgtaag aaaagtggga tcagcaaacc gggagctgct gtggctagtt
    13201 gcagcaaacc atgcagtgaa cccgcctgtg cttcgctata gccgtgactg atgaggatcg
    13261 ccggaagcca gccaataatc acgtaataga ccagtgagtt gatcccaaga aacaatgtta
    13321 cctgctaggc aagtggcgaa cgccagatac cccgagtatg taaggcgcgt gaggtactca
    13381 aatttgcatg ttgctgactt cgccactgtg gcagccataa aaaaagagcc agcagaggaa
    13441 aacacatcag catgaacaac gcgccttgcc agccaaaacc gttcaaagcc agcggcacga
    13501 ccatagccga tcccagtgcc gctgcagctc ccattgtcag ggaatatgcg ccggtaagtc
    13561 tggcgacgga atgagggaaa tcgcgtttaa ttaatcctgg cagtaagaca ttgcctaatg
    13621 caatcccacc gccaatgacc gctgtaccgc caaataataa gtaaggcgaa gggagagaac
    13681 gaattgcgat acctgcacag atcaaaagta acgcggcaaa caggctacgt tccataccaa
    13741 aacgtcgcgc caccggggca gccagaggtg agattagcgc aaaggccaat aatggcaggg
    13801 tggttaataa gccggtttgc gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta
    13861 acggcgctgc gccggtaaag gtgacgcgta atgttgtggc aatcatcaga atgccagcga
    13921 taaggacaat cctgtttttg ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt
    13981 tacaaacgag gctgtacctt accgttttta ttgttatgtt gaatcaagct aaaatgacaa
    14041 attatcgcta agttcggaca aaatgatgca caggttgaat ctcaatggct atgaacccga
    14101 ccgacaccat gaagccgcgg tcgcattttg tattcacgcc ggaacggatg agttaaccag
    14161 tcctgtacat cagcaccgca aaggacagct gatactagca ttacatggtg caattacctg
    14221 tacggtggaa aatgctttgt ggatggtgcc gccacaatat gcggtttgga taccgggcgg
    14281 ggtgaagcat agcaatcaag tgacagctaa cgctgaactc tgttttctgt ttatcgaacc
    14341 cagcgcagtg acaatgccga caacatgttg cacattgaaa atatcgccct tatgccggga
    14401 attaatttta accctggcaa acaggacgac gactcagcgt gctgagccga tgacccgcag
    14461 attaattcag gtcctgtttg atgaactccc tcagcagcca caacaacagc tgcacctgcc
    14521 cgtttcttct catcctaaaa tccgcacgat ggtggagatg atggcgaaag ggcccgtcga
    14581 gtgggggtca ttggggcaat gggctggctt ttttgcgatg agtgaacgca atcttgcccg
    14641 actgatagtg aaagagacgg ggttgagttt tcgccagtgg cgtcagcagt tacagctgat
    14701 tatggcgctt cagggattag tgaaaggcga tactgtacag aaagttgctc atacgctggg
    14761 gtatgattca acaacggcct ttattactat gtttaagaaa ggcctagggc aaacgccagg
    14821 ccgatatatt gcgggattaa ctactgtttc cccacaatca gcgaaaccag acccgcggca
    14881 ataagcgggc caaccggtac gccgcgaaac agcgctacgc ctaaaacagt gcccaccagc
    14941 agcccagcaa ccagttgcgg ctggctgccc attaacgtca cgccgcgccc acccagccag
    15001 gagacaataa cgcctactgc aattgccacc agcgatttcc agttcagaaa cgagtgaatc
    15061 aaggttgagg gcggtagcgt cccgctggcg atgggggcca taacgccaat cgtcagaatg
    15121 attatcccga tacttagccc ctgtttttca atccacggga aaaaggtgct taacggtgtg
    15181 acgcggacaa tgatcaacac cagaattgaa acggcgacag tggtgttatg actgataaag
    15241 cccagcgcag ctaacccgag caggatcagc aaagtgacat caaacatcgt ggtttccttg
    15301 ccaaaaaata ataagcctgc ttactttacg cttgctgcag gaaggaagta gaaaatcttt
    15361 acgttttttg cataaaaaat caggcgaact gtcatcaaac tgccatttgt gcacgctaac
    15421 aataacgtga aaacagctat ctcccggggg catgctatga acgatcaaat gttcgtcgag
    15481 acactgatta tcacatcatc gttttttgct attgcggttg tgctggtttt gtccgttctg
    15541 cttattgaac ggataggcta gctacgcagt gcggcggaaa ttgaccagtt ctggttgcgc
    15601 aatgcgcaaa taatccgcgg ttttcaaaat aacggattta tccagcatcc ctgtattgaa
    15661 ggcgatttca tcaaatcgtt cgaagagtag agggtcggca accagtttga gttttggatg
    15721 gaagctgaaa ggggggatcg cgccgaagac acagcctgtg agttcatcaa cttccgccgg
    15781 gctggccaac gaagcgcgta atccgcctat atggctggcg agttggctca gatcggcctg
    15841 ttgatcggct gcgaggatcg ccagaacatg ttgatttacg ccattgcctt tgactttaca
    15901 taccagtgcc ttagcaccct ggcctagagc ggtgccacga atttctgaca ccgcttcaca
    15961 tttacctacc gcttcatggg taacgacgcg gaagttagca ccttcttgtg ataataaagc
    16021 gattaactgc tgatgcgtca cgcttccttt agccatttca gtcatttcac gctcctgaga
    16081 ttacaagcaa acaaccacag aaggttatca tgaacggctg tttttgttct gcttattgac
    16141 atataaacgc tcatcggaag ctttatacgc atcatgtaac gtaatcgcta aatccccctc
    16201 ctgatgaccg aggttgtcat ttatttgctt taacttgtcc atgtcaattg caataaacat
    16261 caccgaagaa ccggattgca ccagtttctg caaccgctgc tccagttcag gggttaaaat
    16321 tttgcgatta tacagtccag tcatcgcatc actaatattt tctcgcgaca cattttggta
    16381 taaacggaag tgcatccgga ccatattcag caaaattaac gccgtcaaaa tccagaacag
    16441 aacgcttttc cacgaagacg tgataaagta aagaatatca atggataacg agacacgaat
    16501 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
    16561 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
    16621 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
    16681 cacaatccct tttagctgcc ctgcgacata taccggcgtc agcaaactca tcacgtgctg
    16741 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
    16801 aatatctttg cgatcgatgc caatacttcc tgactgcagg aaggaccagt tattcatcgc
    16861 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
    16921 atagtgaaat ttctcttcgg ctatcccaag acctgaaccg taatcatcct ggtggcggtt
    16981 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
    17041 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatggg cagtcaggtt
    17101 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
    17161 cgacacttcc gactgctcag cag
//
LOCUS       DADLXX010000086        17121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLXX010000086 DADLXX010000000
VERSION     DADLXX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.087"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(467..1612)
                     /locus_tag="JNX27_002715"
     CDS             complement(467..1612)
                     /locus_tag="JNX27_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883872.1"
                     /translation="MCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSA
                     KDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPK
                     NNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAH
                     SHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQS
                     DISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGC
                     KSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLF
                     DIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(1770..2108)
                     /locus_tag="JNX27_002716"
     CDS             complement(1770..2108)
                     /locus_tag="JNX27_002716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883873.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="JNX27_002717"
                     /gene_synonym="yiiD"
     CDS             complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="JNX27_002717"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7883874.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="JNX27_002718"
     CDS             complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="JNX27_002718"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7883875.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4118..4990)
                     /locus_tag="JNX27_002719"
     CDS             complement(4118..4990)
                     /locus_tag="JNX27_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY7883876.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="JNX27_002720"
     CDS             complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="JNX27_002720"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7883877.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5682..6467)
                     /locus_tag="JNX27_002721"
     CDS             complement(5682..6467)
                     /locus_tag="JNX27_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7883878.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="JNX27_002722"
     CDS             complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="JNX27_002722"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY7883879.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7565..8461
                     /gene="yihU"
                     /locus_tag="JNX27_002723"
     CDS             7565..8461
                     /gene="yihU"
                     /locus_tag="JNX27_002723"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7883880.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8485..9363
                     /gene="yihT"
                     /locus_tag="JNX27_002724"
     CDS             8485..9363
                     /gene="yihT"
                     /locus_tag="JNX27_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY7883881.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQPGDIVDEMMAKRR"
     gene            9380..10621
                     /gene="yihS"
                     /locus_tag="JNX27_002725"
     CDS             9380..10621
                     /gene="yihS"
                     /locus_tag="JNX27_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY7883882.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLFDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10735..11661
                     /locus_tag="JNX27_002726"
     CDS             10735..11661
                     /locus_tag="JNX27_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY7883883.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11860..13896
                     /gene="yihQ"
                     /locus_tag="JNX27_002727"
                     /pseudo
     CDS             11860..13896
                     /gene="yihQ"
                     /locus_tag="JNX27_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            13942..15327
                     /locus_tag="JNX27_002728"
     CDS             13942..15327
                     /locus_tag="JNX27_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883884.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTLYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15370..16773
                     /locus_tag="JNX27_002729"
     CDS             15370..16773
                     /locus_tag="JNX27_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7883885.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            16841..>17121
                     /locus_tag="JNX27_002730"
     CDS             16841..>17121
                     /locus_tag="JNX27_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883886.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         4267 a         4338 c         4116 g         4400 t
ORIGIN      
        1 gggatattta tttgcataat ctgaatggtg tagccgaaaa acgtcagcat cctaacagca
       61 ccaggattta aggtgaaatt aatctttcat cacagaccgg tcagtactgc caccaaccgg
      121 tctttacttg ctcatcgtta taattttgtt ggtaacagtt cgggaaacaa ggaattcctt
      181 ccggaacacc cccggcatca tccgatagca ttgacgtcaa tcatcagcgc gacgatgagt
      241 ttttttaatc gcatcatagg gtggcgacgt tcagcacgat tacgggtcat agcaaaactc
      301 ctgagcaggc ggccactatg ccgctttcac tggagttctg aatactggta ctttctgtat
      361 ggcttcgcca tgcccctcgc ggctaagtac ctgcacacat ctctctttgt atgtcatttt
      421 tgcaatgcat tgtccgaatt ctgataatcc taatgagttt tactcgtcag actcccatcc
      481 ctacggtata cgagttatat tcatgaatga aatcgacatt tttaaaaaac gaatcttgta
      541 aaacatcagt gttgatatta ccttgcaatc caacattgaa tttgttagag acatgaagaa
      601 ctcgaatatc aaaaaggttt tttaacatca ttaattcttc gggtgttaat gaatcgcaac
      661 taaaatatcc ctgttgatca aaaagatttt ctatttctcc actatcggcg aggcgttttt
      721 caaggagtgt ttgcaacatc gagttgccaa ggacaatgga tatcgtgcga tctttcgcag
      781 atttacaccc catcacaacg gcaacattcg tatccccctc aagagtttca tttatcgccg
      841 tcgctaaaat ggtggtcaaa cacgcaaacg gaactgtatt tctttcgtga tttaatttat
      901 ctaccagttc attccattta tcaaaagccg ggctatattt gttgcgcaca gaaatatccg
      961 actggttaaa caattcagcc atgcgatttt ttatcatctc gatatccggc gaaccagaaa
     1021 gactttgcgc ataatacggt cctgatgatg ttgcatacat taatgccaag cccacacctg
     1081 tcgccatgat gggcgcagcc acggttttgg caagggcgac gctgcgatgg ctatgcgctt
     1141 cacaggctaa aggaacatat tccactgact gcggtaattc cttacatgcc tccctcatca
     1201 ttgcggtatg ctttacaaac aacggttttt cactgcccaa aaaaactttc tgcttaattg
     1261 cactaagaaa acgcaaatca aaaataattg tttttggttt attatttttt ggcacatttc
     1321 ctattatccc gctaagaacc ttccttgctg catggagatt tttttgcaca gcacataaca
     1381 caattgatga agccttctcc actgcattat tgattttaat tttaaaatcc gcatgatata
     1441 cgttgacggg caatgcaagt atatctggac ctacatcttt tgctgagtat ttattggtga
     1501 tcgacggacg tgaacccacg agaaaactta gctgcaaatt atgctctata ttatcagctg
     1561 tttttgtggt gtggttaaac atagagatgc cacctttcat tctttcacac atatcctcaa
     1621 ctttttcact tcgttcttgt ggcaatttgg ctttaacatt aatttgatga tttttacccg
     1681 tgcattcctt aactacaagg ggaatgttat ctaaagtgcc atacaggcta ataacgtcac
     1741 caggattttt atcatgtaag taagtttgat taatacttga agcattcaca aagagggtca
     1801 atattataac tgtttataac ttcacctaca tcaagtgtgt agtgcttact ctggagactg
     1861 ccatccttat ttaattgtat atcaatcaat atatccccgg actttgtcac catatctttg
     1921 ccataaacca tttgcatagc catttcgagg agatttttat ctaactgatt gcgggcgttg
     1981 agtaagcgat taccaaataa tttatatact gttcccgtca ttgccagcgc tgcgctttct
     2041 ggtactttgc gtttccacag tgaggtttct ctatttttaa acaggtcata ttcattatta
     2101 ctcgacaaac catgcttcag ttcgttaaca caatcgcaca gttgctcctc accttccatt
     2161 ttaaggcaaa gtgaaaattg atttaatggc gtttacttcg gtgatttatt actcattagt
     2221 tgtttttcta aattaacaac agaataactt tcttgtttaa tgagattaat attggcaacc
     2281 attatagcct ccttcccacg gtttaagagc aaacatacaa cataatgatt cataacatac
     2341 agagcagtaa atgaatgata ctgacaatgt atgccagccc attatctttg cataatgttt
     2401 gtgagctata gattttaaaa atatttatga aaaaagtatg gcaccagagg gggttttgac
     2461 tatcaaaaat cgctcaaacc tgaactaaga tgatgagtta agcattatct gcaataattg
     2521 ccactcagct ctacaaataa tatttaaatg ctcctgaaca gtgatgtagc aagacacgta
     2581 tggtgccaac gaagaataaa aatttataaa gttcttattc aatataaccg gaatagtcag
     2641 ggactgatgt aaatgaccgg aatgaatgag agaggcagca ccgcccctct ccataagcta
     2701 ctcttcttcg ttcccgccct cttcatacgg gccaaatggc ttcgcgggca gaacgatata
     2761 cgtgccttca aacactgcac ccggcgtctc gtcgccaaag atttcgacct gcatctgcac
     2821 ccgtgctttt cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac caaggtcggc
     2881 taccgcatgg ggtttaccgc taatcggttt gctgtagcgg atatgcgcat ccgccagaat
     2941 aatcgttccg ccgaggtggc gttcacgcag catcagccag ataagccccc aaccggtgag
     3001 cgtcgccagc gagaataaac tcccggcaaa cagcgtatgg tgcggattct gattgccggt
     3061 ttccggcatg gtggtgataa atttttgccc ggtatattgt tgaatacgca cgcccatttt
     3121 ttcactaagc gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc agtcgccgcg
     3181 atgcaaaata tcatccagag tggcgacggg cttaatcatc aaaaaatggc gaatcggcgt
     3241 ggtggttggc gttgtgattt ctccctgatt aacaaacccc agcttggcga aaaactccac
     3301 cgcgtcttca cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc
     3361 cagggtcatc gccatcagcg ttcctaaccc tttgtcctgc acgtccgggt gaacggccat
     3421 aaagcgaatg gacgcttcat tgtcggcatt aatatacaat cggcctaccg ccaccagatt
     3481 accctgctcg tcgacgacca tctggtgatg cgccatcgca tcccacgcgt cgcgttccga
     3541 accttttggt tgatgcaggg gcttacgcaa catttcccag cgaaactgat agtaacgctc
     3601 taactcttct tctgtttgtg gaacctgaag gtgatacata gcgatactct ctcttgtttc
     3661 ccgtgacaac cctggaagct ggctcatacc tgcaaccaga atgtcacggg gccatcattg
     3721 accagcgata cctgcatatc ggcagcgaag cgtcctgttt gcgtgttcat ctcttgctgg
     3781 cggcagcgtt cgacgaaata gtcatataac gcctctgcgc gatccggtga tgcacctttg
     3841 gagaaacttg ggcgcatccc ccgttcggta tctgcggcga gggtaaactg ggaaaccacc
     3901 agtacactgc cgcccgcctg ttgcacgttg agattcatct tgccttcggc atcgctaaag
     3961 atgcggtagc cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt
     4021 tcgacaccca ataacaccaa aagtcccgcg ccaatttcgc ccgtcacttc tccctccacg
     4081 gtgacgctgg cacgggttac gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt
     4141 gttcagctgc ttgttttagt ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa
     4201 gcaagacgat acaccatgtc cagtagaccc aaacaaagag aatggggatc accgccagca
     4261 caccgtaaat gagctgatat gacgggaaca tggtgatata aagcgcgaaa cctttctttc
     4321 ctgcttcgaa cagaagtgcg gcgacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc
     4381 ggatggtagg aacaatgctg tacagcaacc agaaggagat ccacgacaac agcaacggaa
     4441 aaatacgcag cacgttgtcg atgacagtat tgagatcgct cgcccagcgc agggagagca
     4501 aataggaact gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc
     4561 agtacacggc gaacgagtaa atttttggtc gcgctcgttt actgcgccag atggtattca
     4621 acgcgctatc gatggagtac atcaacaata acgcggtgac gatcagcccg cacgccccaa
     4681 cggcggtcat cttgttggaa ttagcaacaa attgttcgat ataccgctga ataacatcgc
     4741 cagtagcagg cagaaagttg gcaaaaataa agtgacgtaa ctgaatgctg acgtcggaaa
     4801 acatgggaaa agcggcgaaa agcgcaaaaa caacggcaac cagcggcact aatgagagca
     4861 acgacacata ggcaaggtta cctgccaggg ttgtcatgtt gtcctcatca atacgttgcc
     4921 agagtagttt tagccaggcc catagtggac gggtacggtg cctggctttg tcctgaatgg
     4981 tttttagcat aacaccttcg cgaaatagtc cgggatggtg gttttatctt tcaccagaat
     5041 actggtaatg cctagctgat tagctccttc tatattatcg gcgttatcgt cgaaaaagac
     5101 cgtatcgctg ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc gtgcttcagg
     5161 tttgcgcatc cccagatctt gcgacagata gatatggtca gcagcatcac gaatttccgg
     5221 gtattcttcc ggccagaagg tggtatgcag gcggttggta ttggaaagca ccaccacgcg
     5281 atgcccctgc tcacgcagtt tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa
     5341 caccgcctgc cagccgtgag agaactgctc gtagcttagc ggtagagcca tctcatgaca
     5401 cagcgcctct gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc
     5461 ccccatatga aaactcttct taagcgatgc cagcggaata cgcgttaaat cgctccaggc
     5521 tcccagcaca cggttaaagt cgatatcgac aatcacatta cctaaatcaa agatatagag
     5581 catttttgcc tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga tgggtttggc
     5641 tatgtggcaa ggtatgaagc caggttaacg gtcacaaaaa tctacgcgtc ttcctgggga
     5701 agaatgattt caatttggct gtcttgtaag cgtgatttta gcgcagcggg cggcagctcg
     5761 tcggtaaaga gcgcagtgac ctgtgccacg ttaccaattt caaccgccgc cgaagcatga
     5821 tacttagtgt gatcggcgac cagcagaata tttctcgcgt gcgccatcat cgttttcacc
     5881 acgttagctt cgtttacatc aaactccatc aacgcgccat cgctctcaat cgccccaacg
     5941 cttgttacca gataatcagc acgaaaatca gccacaaagg acgccgctga agggccaatg
     6001 atcccgctat tatgagagcg caacgtaccg ccgggcacca tcacttcaaa gcgcgggttg
     6061 tggtaaagaa tatgcgccac acgcaggctg ttggtgatta tccgcaaatg attatggtta
     6121 agtaacgccc gggcaacatg ctcaacagtc gtaccaatgg tgataaatat tgttgaacca
     6181 tcaggaatat agtctgccac cgcttcggca atcgcttttt ttttctcggt ttgcgaaacc
     6241 tcacgctgct cgaacgccgt attaacgacg ctggaagccc gacccgcgcc accgtgatgg
     6301 cgcgtaatca ggccttgctc gcttaattta cgaatatccc ggcggaccgt ctgcgtggag
     6361 acatccagca gatttgccag ctcatcaata ttcatatacc cacgctcggc gatcagcatg
     6421 aggagttggt cgtgccgcgg gttaccggtt agttcggtaa ggctcatgaa aattcctcga
     6481 aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa aagatcgggt ttgatcacag
     6541 tcagggatcc cggctcgtcc accgggacgt gtgcatttta acgccgctac accgctggcg
     6601 aagcggactg actccgctaa atccccactt gttgccagcg ccaccgccaa agcgccgtga
     6661 aaaacatcac ccgcacctgt ggtatctacc acatcaactt tgaaggccgg ttgatgctga
     6721 cgcccaccat tttccagcca gtcgcagcct gcgctaccct gggtcacata gacatgacca
     6781 tttgtgagcg tttgtgcctg ttttagcgca ctggccatct ctttcacgcc cgttaagcgc
     6841 gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg ccaccagctc actgatatcc
     6901 tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg cctgacgggc cagggtgaag
     6961 gcttttttag cgccgtcgtg ccagcgtaca tctgccagca caacatccca ctgagagaaa
     7021 tcaatttcct ccaaccactc tgcgtcaggc agcagatccg ggctggggta gttaatgatt
     7081 atccgctcgc ctttggtatc caccatgatg gcggattgcg aagatttcgc ctggttatac
     7141 cgtttggtgt aacgggtgtt aacgccccag gattccagct ctgctagcag gctgttgccc
     7201 gcgtcgtcat cacctacgcg accaataaaa tcgacctgcg cccccagcct tgccgccgca
     7261 accgctgccg tcgccgctgg cccgccacca acttccgtat aatttctcgc cacgtattta
     7321 ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat ccatcacggt tatacctaca
     7381 caagcaacac gaatcatggt cattccttaa acattttggg atgcgttcat tttcatttca
     7441 ccaattgttt aaaaagtgac ggctgtcaat tttttgacga taaattacaa atgcgatcaa
     7501 aaacagacaa gcaaagcatg tcataaatga caaaaagtga catcactgta ttcaggagag
     7561 ggttatggca gcaatcgcgt ttatcggttt aggacaaatg ggttcgccaa tggcgagcaa
     7621 tttattgcag caagggcatc aacttcgcgt cttcgatgtg aacgccgaag ctgtgcggca
     7681 tctggtagac aaaggtgcga ctcccgccgc caacccggtg caggccgcta aagatgccga
     7741 atttatcatt accatgttgc cgaatggcga tctggtgcgc aacgtgttgt tcggtgaaaa
     7801 cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt gatatgtcca ccatccatcc
     7861 gctgcaaacc gataaattga ttgccgatat gcaagccaaa ggcttcagca tgatggatgt
     7921 tccggtaggc cgtacttctg caaatgccat taccggtact ctgttactgc tggctggcgg
     7981 caccgctgaa caagttgaac gtgccacacc gatcctgatg gcgatgggca gtgagttgat
     8041 caacgctggc ggtccgggca tggggatccg cgttaagctc atcaacaact acatgagcat
     8101 cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc gaagccctga atcttccctt
     8161 cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt aaaggtcact tcaccacttc
     8221 ctggccaaac aaagtcctca gcggagatct ttctcccgcc ttcatgatcg atcttgccca
     8281 taaggatctt ggcatcgccc ttgatgtcgc caaccagctg catgtgccaa tgccgctggg
     8341 ggccgcctca cgggaggttt atagccaggc gcgcgcagcg ggtcgcggtc gccaggactg
     8401 gtccgccatt ctggaacagg tccgtgtcag tgccgggatg actgccaaag taaaaatgta
     8461 acgactggat aaaggaataa atgaatgaat aagtacacca tcaacgacat tacgcgcgca
     8521 tcgggcggtt ttgccatgct ggcggtcgat cagcgcgaag ccatgcgcat gatgtttgcc
     8581 gcggctgggg cacccgctcc ggtagccgat agcgttttaa ctgatttcaa agttaacgct
     8641 gcaaaggccc tctcgcctta tgcctcggcg attctggtag atcaacaatt ctgctaccgc
     8701 caggtggttg agcaaaacgc gattgccaaa agttgcgcca tgattgtcgc cgccgatgag
     8761 ttcattcctg gcaacggtat tccggtcgat agcgtggtta ttgaccgcaa aatcaatccg
     8821 ctacagatca aacaagacgg tggcaaagcc ttaaaactgc tggtgctgtg gcgtagtgat
     8881 gaagatgcgc agcaacgtct ggatatggtg aaagagttca acggactgtg ccactcacac
     8941 ggtctggtaa gcatcattga gccagtcgtc cgcccaccgc gtcgtggcga taaattcgat
     9001 cgcgaacaag cggtcatcga tgccgccaaa gagctgggcg acagtggcgc tgacctctac
     9061 aaagttgaaa tgccgcttta tggcaaaggt ccgcaacaag agcttctctg tgcttcacaa
     9121 cgtctgaatg accatatcaa tatgccatgg gtgatcctct cttccagtgt cgacgaaaaa
     9181 ctgttcccgc gtgccgtacg cgtggcaatg acggcgggcg catcgggatt cctggcaggc
     9241 cgtgcagtct gggcatcggt cgtcggttta ccagacaacg agctgatgct ccgtgacgtt
     9301 tgcgcaccga aattacaaca acctggcgat atcgtcgacg aaatgatggc taaacgccgc
     9361 taagatgagg ataaaaggaa tgaaatggtt taacacccta agccacaacc gttggctgga
     9421 acaggaaacc gaccgcatct ttgattttgg taaaaattcc gtagtgccga ctggttttgg
     9481 ctggttaggc aataaagggc aaatcaaaga agagatgggc acccatctgt ggatcacagc
     9541 tcgtatgttg cacgtttatt ccgttgctgc ggcgatgggt cgacctggcg cttacgcgtt
     9601 ggttgatcac ggtattaaag ccatgaacgg cgcactgcgt gataaaaaat atggcggctg
     9661 gtatgcctgc gtgaatgacg agggcgtggt ggatgcctcc aaacagggct atcaacactt
     9721 ctttgctctg ctgggtgccg ccagcgccgt cacaacgggt cacccggaag cgcgcaagct
     9781 gttcgattac accattgaga ttatcgagaa atatttctgg agcgaagaag agcagatgtg
     9841 cctggaatcc tgggacgaag ccttcagcaa aaccgaagag taccgcggcg gcaatgccaa
     9901 tatgcacgcg gtggaagctt tcttgattgt ttatgacgtc actcacgaca aaaaatggct
     9961 ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac gtcgccagaa ataatcatta
    10021 tcgcgttaac gaacacttcg atacccagtg ggatccgctg ccggattaca acaaagataa
    10081 cccggcgcat cgcttccgcg cgttcggagg tacaccaggc cactggatcg aatggggccg
    10141 tttaatgctg cacatccatg ctgccctgga agcccgttgc gaacaaccac cagcatggct
    10201 gctagaagat gccaaaggtc tgtttaacgc caccgtgcgc gatgcctggg cacccgatgg
    10261 cgcggacggg attgtttata ccgttgactg ggaaggaaaa ccggtggtcc gcgaacgtgt
    10321 acgttggcct atcgtcgaag caatgggtac ggcctacgcg ctctacaccg tcactggcga
    10381 tcgccagtat gaaacctggt atcaaacatg gtgggagtac tgcattaagt acctgatgga
    10441 ctatgaaaat ggttcctggt ggcaggagct ggatgcggac aataaggtca taaccaaagt
    10501 ctgggacggc aaacaggata tttatcacct gctgcattgc ctggtgatcc cgcgtatccc
    10561 gttagcgccc ggcatggctc cagcggttgc agcgggtctg ctggatatta atgcgaaata
    10621 actgatctga aaatagggtg cgggttttcc cctcacccta accctctccc caaaggggcg
    10681 aggggaccgt ccggtgcagt ttttgggttt cacatcagcc tcacgggagg ctgtatgtcg
    10741 ttaattaagg tgccttgtat gcaaatcacc aatatgcatt gcagtggaca gaccgtttca
    10801 cttgccgcgg gggattatca cgcgaccatt gtgaccgtcg gtgctggtct ggctgaatta
    10861 acctttcagg gttgtcattt agtcatcccg cacaaacccg aagagatgcc gctggcgcat
    10921 ctgggtaaag tgcttattcc gtggccgaac cggatcgcca acggctgtta ccgttatcag
    10981 gggcaggaat atcagttacc cattaatgaa catagttcaa aagccgctat tcacgggctg
    11041 cttgcctggc gagactggca aattagtgaa ttaaccacca ccagcgttac cctgacagct
    11101 tttctgccgc ccagttacgg ctatccgttt atgctggctt cacaagttgt ttattcgctc
    11161 aacgcacaca cgggtctttc cgttgagatc gcaagccaga atatcggcac tgtcgcagcg
    11221 ccttatggcg tgggtattca tccttattta acctgcaact taacatctgt tgatgaatat
    11281 ctgttccagc ttccggcaaa ccaggtttat gccgtcgatg aacacgtaaa tccgacaacg
    11341 cttcaccacg ttgatgagct ggatttaaat ttcacgcagg cgaaaaaaat agccgcgaca
    11401 aaaatcgacc acacctttaa aaccgctaat gatttgtggg aaatgaccat tacccatcca
    11461 cagcaggcgc tttcggtaag cctttgttcc gatcaaccct gggtgcaggt ttatagcggt
    11521 gaaaaattac agcgtcaggg actggcagtt gaacctatga gttgcccgcc taacgccttt
    11581 aattcaggta tcgacttatt attactggaa ccaggaaaac cgcaccgcct gttttttaat
    11641 atttacggac agagaaaata acttgtattg attagcagtc aaaatcattg aagaacattt
    11701 cactcgctaa aggcaacatt atgtatcttc aatttattaa gttaaaaaca gctcagcttc
    11761 tgtaaagttt ttatatatca gttttatgag aatcatttta cataagcctg taataacagg
    11821 aaggcaacaa tctcgttctc aactctatgg agattagtta tggatacgcc acgtccacag
    11881 ttattagatt ttcaatttca tcagaataac gacagtttta ccctacattt tcaacaacgt
    11941 cttattttaa cccatagcaa agataatcct tgtttatgga ttggctcagg tatagcggat
    12001 atcgatatgt tccgcggtaa tttcagcatt aaagataaac tacaggagaa aattgcgctt
    12061 accgacgcca tcgtcagcca gtcaccggat ggttggttaa ttcatttcag ccgtggttct
    12121 gacattagcg ccacgctgaa tatctctgcc gacgatcagg gccgtttatt gctggaacta
    12181 caaaacgaca accttaacca caaccgtatc tggctgcgcc ttgccgctca accagaggac
    12241 catatctacg gctgcggcga acagttttcc tacttcgatc tgcgcggcaa accgttcccg
    12301 ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa cctatgtcac ctggcaggcc
    12361 gactgcaaag aaaatgcggg cggcgactat tactggactt tcttcccaca gcctacgttt
    12421 gtcagcacgc agaagtatta ctgccatgtt gataacagtt gctacatgaa cttcgacttt
    12481 agcgccccgg aataccatga actggcgctg tgggaagaca aagctacgct acgttttgaa
    12541 tgtgctgaca catacatctc cctgctggaa aaattaactg ccctgctggg acgccagcca
    12601 gaactgcccg actggattta tgacggggta acgctcggca ttcagggcgg gacggaagtg
    12661 tgccagaaga aactggacac catgcgtaac gcgggcgtga aggttaacgg catctgggcg
    12721 caggactggt ctgggatccg tataacctct tttggcaagc gcgtgatgtg gaactggaag
    12781 tggaacagcg aaaactaccc gcaactggat tcacgcatta agcagtggaa tcaggagggc
    12841 gtgcagttcc tggcctatat caacccgtat gttgccagcg ataaagatct ctgcgaagaa
    12901 gcggcacaac acggctatct agcaaaagat gcctctggcg gtgactatct ggtggagttt
    12961 ggcgagtttt acggcggcgt tgtcgatctc actaatccag aagcctacgc ctggttcaag
    13021 gaagtgatca aaaagaacat gattgaactc ggctgcggcg gctggatggc tgacttcggc
    13081 gagtatctgc ccaccgacac gtacctgcat aacggcgtca gtgccgaaat tatgcataac
    13141 gcctagcctg cgctgtgggc gaagtgtaac tacgaagccc ttgaagaaac aggcaagctc
    13201 ggcgagatcc tcttctttat gcgcgccggt tctaccggta gccagaaata ctccaccatg
    13261 atgtgggcgg gcgaccagaa cgtggactgg agtctcgacg atggcctggc gtccgtagtc
    13321 ccgacggcgc tgtcgctggc aatgaccgga catggtctgc atcacagcga cattggcggt
    13381 tacaccaccc tgtttgagat gaagcgcagc aaagagctgc tgctgcgctg gtgcgatttc
    13441 agcgccttca cgccgatgat gcgcacccac gaaggtaacc gtcctggcga caactggcag
    13501 tttgacggcg atgcggaaac tatcgcccat tttgcccgta tgactaccgt tttcaccacc
    13561 ctgaaacctt atctgaaaga ggccgtcgcg ctgaatgcga agtccggtct gccggtcatg
    13621 cgcccgctgt tcctgcatta cgaagacgat gcacatacct attccctgaa atatcagtac
    13681 ctgttaggcc gcgacattct ggtcgctccg gtacacgaag aaggtcgcag cgactggacg
    13741 ctctatctgt cggaggataa ctgggtacat gcctggacgg gtgaagcgtt ccggggcggg
    13801 gaagttaccg ttaatgcgcc catcggcaag ccgccggtct tttatcgcgc cgatagcgaa
    13861 tgggcggcac tgttcgcgtc gttaaacagc atctaatcac atccgcccgt ggggaaactc
    13921 atgggcaaag ggagaacatc gatgagtcac atcacaacgg aagatccagc aactttacgc
    13981 ctgcccttta aagagaaact ctcttacggt attggcgacc tggcctctaa catcctgctg
    14041 gatatcggta cgctttatct tttgaagttt tataccgacg ttctggggct gccaggcacc
    14101 tatggcggca ttatcttttt gatttcaaaa ttctttactg cgtttaccga tatgggtacc
    14161 ggcattatgt tggattcccg acgcaagatc ggtccaaaag gtaagttccg tccttttatt
    14221 ctgtatgcgt cattcccggt caccttactg gcgatcgcca actttgtcgg cacaccgttt
    14281 gatgtcaccg gtaaaacggt gatggccact attctgttta tgctttacgg actgtttttc
    14341 agcatgatga actgctccta cggcgcaatg gtcccggcaa tcaccaaaaa ccccaacgaa
    14401 cgcgcctcac tggcggcatg gcgtcagggc ggcgctacgc tgggcctgct gctgtgcacg
    14461 gtgggattcg tgccagttat gaatcttatc gaaggtaatc agaaacttgg ctatatcttc
    14521 gccgccacgc tgttttcact gttcggcctg ctgtttatgt ggatctgcta ctcaggcgtg
    14581 aaagagcgtt atgtcgaaac ccaaccaacc aatccggcgc aaaagcctgg cctgttgcaa
    14641 tctttccgcg caattgccgg taaccgccca ctgttcattc tgtgtattgc caacctctgc
    14701 accttagggg cgtttaacgt caagctcgcc attcaggtct attacaccca gtacgtactt
    14761 aacgatccca tcctgttgtc gtatatggga tttttcagca tgggctgtat tttcatcggc
    14821 gtgttcctga tgcctggcgc agtcagacgt tttggtaaga agaaggtcta tatcggcggc
    14881 ctgctgattt gggtgctggg cgatctgctc aactatttct tcggcggcgg ctcggtcagc
    14941 tttgtggcgt tctcctgcct ggcgttcttc ggctcagcgt ttgttaacag cctgaactgg
    15001 gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc
    15061 acgctctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc tggtttcttc
    15121 cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc tgaccacacc
    15181 attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt agtcaccatt
    15241 gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat tgtggaagag
    15301 atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg ggcgttataa
    15361 ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg ggaaaaaatc
    15421 gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac tctgtatctc
    15481 ctcaaacttt ataccgatga attagggatg cctgcttact atggcgggat tatctttctg
    15541 gtcgcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact cgactcgcgg
    15601 aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc agttccggcg
    15661 gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt taaaacgacg
    15721 attgccaccg cgctgtttat gatgtttgga ctttcataca gtctgatgaa ctgctcgtat
    15781 ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac
    15841 cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat tccgttgcag
    15901 tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt ctccattggc
    15961 ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt ggacacagcg
    16021 ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg taatccacca
    16081 ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat caaactggcg
    16141 attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc gtggatggga
    16201 ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt
    16261 tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg tgatatactg
    16321 aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt
    16381 ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt agattacggt
    16441 gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac cttctttcgt
    16501 aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca aattggttat
    16561 gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct catttttatc
    16621 tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta cacactcaat
    16681 gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa agagatggcg
    16741 acagaaaaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat ttccatcctg
    16801 gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat
    16861 aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag
    16921 ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg gttataactt
    16981 cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag aagatgaact
    17041 aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt
    17101 aaccatccag cccggtggct t
//
LOCUS       DADLXX010000087        17085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLXX010000087 DADLXX010000000
VERSION     DADLXX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.088"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNX27_002731"
     CDS             complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNX27_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7883887.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="JNX27_002732"
     CDS             complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="JNX27_002732"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7883888.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="JNX27_002733"
     CDS             complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="JNX27_002733"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7883889.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="JNX27_002734"
     CDS             complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="JNX27_002734"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7883890.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4949..7495
                     /gene="cyaA"
                     /locus_tag="JNX27_002735"
     CDS             4949..7495
                     /gene="cyaA"
                     /locus_tag="JNX27_002735"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7883891.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENSFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="JNX27_002736"
     CDS             complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="JNX27_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7883892.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7903..9216)
                     /locus_tag="JNX27_002737"
     CDS             complement(7903..9216)
                     /locus_tag="JNX27_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7883893.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9213..9518)
                     /locus_tag="JNX27_002738"
     CDS             complement(9213..9518)
                     /locus_tag="JNX27_002738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883894.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEVTK"
     gene            complement(9540..10691)
                     /locus_tag="JNX27_002739"
                     /pseudo
     CDS             complement(9540..10691)
                     /locus_tag="JNX27_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10880..11083
                     /locus_tag="JNX27_002740"
     CDS             10880..11083
                     /locus_tag="JNX27_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7883895.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11120..11944
                     /gene="dapF"
                     /locus_tag="JNX27_002741"
     CDS             11120..11944
                     /gene="dapF"
                     /locus_tag="JNX27_002741"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7883896.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11941..12648
                     /locus_tag="JNX27_002742"
     CDS             11941..12648
                     /locus_tag="JNX27_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7883897.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12645..13541
                     /gene="xerC"
                     /locus_tag="JNX27_002743"
     CDS             12645..13541
                     /gene="xerC"
                     /locus_tag="JNX27_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7883898.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13541..14257
                     /gene="yigB"
                     /locus_tag="JNX27_002744"
     CDS             13541..14257
                     /gene="yigB"
                     /locus_tag="JNX27_002744"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7883899.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14341..16503
                     /gene="uvrD"
                     /locus_tag="JNX27_002745"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14341..16503
                     /gene="uvrD"
                     /locus_tag="JNX27_002745"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7883900.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16650..17042)
                     /locus_tag="JNX27_002746"
     CDS             complement(16650..17042)
                     /locus_tag="JNX27_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883901.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGPMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3956 a         4428 c         4534 g         4167 t
ORIGIN      
        1 ttgatgctgt ttttagtttt aacggcaatt aatatatgtg ttattaattg aatgaatttt
       61 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
      121 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      181 ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
      241 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      301 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      361 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      421 atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt ggcgggttat
      481 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      541 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      601 ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt gactgaccca
      661 atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt agcacttttt
      721 ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
      781 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      841 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      901 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      961 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     1021 ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata tacgcctgtt
     1081 ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1141 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1201 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     1261 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     1321 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct tccgccagtt
     1381 tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg acaaaccatc
     1441 cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1501 ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg atattgtagt
     1561 tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca accactatcc
     1621 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1681 ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtttagc
     1741 ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct gcatcagttt
     1801 ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat tttgctggct
     1861 taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat cgtagtaagc
     1921 acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct gatgacgcgg
     1981 tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat agatatcctg
     2041 atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa tgaagttgtc
     2101 cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg aactggaaag
     2161 ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca gacgcaggtt
     2221 atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat ccacctgcga
     2281 tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa tcagactcgg
     2341 gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag aggtcgtgac
     2401 gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg cctgagccag
     2461 cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga cttcatccaa
     2521 ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag cttgttgctt
     2581 aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg ccgtcagttg
     2641 gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac cccagccata
     2701 caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa tcaatgcagt
     2761 attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca cggcctccct
     2821 ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt cctgttatga
     2881 gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga ccttaatgtc
     2941 ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca atagtcgaca
     3001 gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt gctgcaacat
     3061 ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca tcgcttcttc
     3121 tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga cctcagcacc
     3181 gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta atatcagcgc
     3241 acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc tgatttcccg
     3301 atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg cggtggtgcg
     3361 tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat gcagctgtga
     3421 ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct cccccagcgc
     3481 tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa tcagcggaaa
     3541 atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc cagcgggaga
     3601 cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc agcgaggatc
     3661 tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg ttcggcatct
     3721 tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc gccgaccagc
     3781 gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg cacctgacat
     3841 ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc agtttcgtgg
     3901 tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca ttcaataccg
     3961 actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc gcgaatgcgg
     4021 gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc gtattcgccg
     4081 ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat aatcagatcc
     4141 gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac gatactgcct
     4201 gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg aggatcttca
     4261 cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac atctttcatt
     4321 gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc ttttacaaat
     4381 aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc gcgcgtcacc
     4441 atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc tttgacatag
     4501 tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac attgtctaac
     4561 atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca gagtgatgtc
     4621 agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg gcagtggatc
     4681 ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg gtgctacact
     4741 tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga tcacgtttta
     4801 gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa cggttacctt
     4861 tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga atcacagtca
     4921 tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc tgaaacagag
     4981 actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg ggcctgcatt
     5041 ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc taatgccggg
     5101 ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg aaactcaacg
     5161 ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc caccgaaagg
     5221 tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag ggcaaagttg
     5281 ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg aagagcgcca
     5341 gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg gtgtggaagt
     5401 cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca gcctgggggg
     5461 cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc gtactgccgt
     5521 gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag aagagcatta
     5581 cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg aatggctgga
     5641 tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc tttggcagct
     5701 ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc tggaagccta
     5761 ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc gtttgcacga
     5821 cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc gtgttactga
     5881 atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct gcttctattt
     5941 aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc gcgcagtgtt
     6001 gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc tcgataaccg
     6061 tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg acgcgatgat
     6121 gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg tctccgccag
     6181 tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag cattgccagg
     6241 taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga atctgacctt
     6301 tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata accgcgcgcc
     6361 aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc tgaataaact
     6421 ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt atattaaagg
     6481 taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt cgcaccattt
     6541 cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg agattcgcca
     6601 tctggcgatt atcgttaacc tggaatatga ccctacagct gcgttccgca atcaggtggt
     6661 gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa attgcctggt
     6721 aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc tgcacttcaa
     6781 cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc atcaggacgc
     6841 cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg gcttaatccg
     6901 tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca gcacccgcca
     6961 ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct tgttcttcga
     7021 acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg gcgctatttc
     7081 gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca aattaccggc
     7141 ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag aaacgcaaga
     7201 cgagaatagc tttaatatct acattctcga cgaaagcaac cgggttgagg tgtatcacca
     7261 ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact cgtcatcgca
     7321 cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt tctatcagat
     7381 tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga ttggcaacct
     7441 gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt cgtgatgagc
     7501 gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact gtttcacccg
     7561 cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg cgatcgcaaa
     7621 tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc agccacacct
     7681 ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag gtaatggtca
     7741 gtacgccgcc gttgatctcg cagtcgatat cgctgtcgcc atccctgtcg tccagacgct
     7801 cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg ttcattgttg
     7861 taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct tcaaagtttg
     7921 cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga tgtctctgtt
     7981 ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg gggagggcgt
     8041 aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag cgaagcgatc
     8101 gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc gtaattggcg
     8161 gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca agtctggcgt
     8221 atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg ttacatacag
     8281 ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag gttcaatacc
     8341 tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac gccagcgaac
     8401 gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt tgcctgataa
     8461 ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca gtgaatgagg
     8521 tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc catttaaata
     8581 aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt ttacactcat
     8641 aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac tgctgctccc
     8701 ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag agttaccttc
     8761 gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt cggtgcgctg
     8821 tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct gaaagctggc
     8881 aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc agagaataac
     8941 gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat cggaaggtat
     9001 ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc tcatctccgg
     9061 aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca taacaccgtg
     9121 atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg tgtgtcccaa
     9181 caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt gcgcggggca
     9241 ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt gtaatttacg
     9301 ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt cggatcagcg
     9361 gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt atcgcgggca
     9421 taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc cggagaacgg
     9481 ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga atggtatggc
     9541 tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa acgtttgttg
     9601 ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc agactctgtt
     9661 tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat gggttgaaac
     9721 tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt acgccagaac
     9781 actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc atcccacgcc
     9841 agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca ggtcaagagt
     9901 gagccgggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag ctgttcgccg
     9961 ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa tggtacggca
    10021 atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt ctgcgccagc
    10081 acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa attagcgtca
    10141 cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac ctgatgcagc
    10201 gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc ttcaaaatag
    10261 agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac atcgacggga
    10321 ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg accatcgcta
    10381 tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc ggttatcaaa
    10441 tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat taataaattg
    10501 ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt ttctttataa
    10561 gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc aggtgcggaa
    10621 agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca aaccacattg
    10681 cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc agcagaacat
    10741 aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga cttcataaag
    10801 agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag aaagcgatga
    10861 actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat tacttactct
    10921 cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg cagataaaaa
    10981 cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc cggataaaaa
    11041 cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag tttctgtacc
    11101 cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca acgattttat
    11161 ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc gtcgcctggc
    11221 ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc cgtatgatcc
    11281 tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg
    11341 taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca ataagcgtga
    11401 tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg atgatctggt
    11461 ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc gcgctaacaa
    11521 agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc
    11581 gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg cggtagaaac
    11641 gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata tcggttttat
    11701 gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg caggagaaac
    11761 ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc aaggtttgct
    11821 ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc
    11881 gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat ttattcatct
    11941 atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga ccgggcggtt
    12001 gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc agtagaagcg
    12061 atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca catggcccgc
    12121 gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga gcaggctatc
    12181 gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac ggccgccagc
    12241 agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg cctggctggt
    12301 gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa ccacactcat
    12361 ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag
    12421 cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga agcgaaagcg
    12481 gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt tgtgctgttt
    12541 accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact tcatgaaatt
    12601 gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc gatttacaca
    12661 ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc ccgataaccc
    12721 tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa aacggcctgc
    12781 aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc agtcgccgta
    12841 aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc ttttttgact
    12901 ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg gcaccgaaag
    12961 cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg ctggatattg
    13021 atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg tacggcgcgg
    13081 gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg gagtccggcg
    13141 aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt cgcaacgctg
    13201 tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc
    13261 tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg
    13321 aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta cgtcactcgt
    13381 tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag ctactgggtc
    13441 atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac cttgcctcgg
    13501 tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt accggccttt
    13561 ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata accgtccggt
    13621 gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg
    13681 cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag cggaacccga
    13741 gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga tgctcgacgc
    13801 cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta actttgccaa
    13861 atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac agctggcgaa
    13921 gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt ttggtttggg
    13981 ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga
    14041 tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg
    14101 ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg cttgttggat
    14161 cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc cacatctgga
    14221 aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt atatataccc
    14281 agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc ggcggtgcca
    14341 atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc ggtggccgcg
    14401 ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg
    14461 catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat tatggctgtg
    14521 acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact gatgggcacc
    14581 agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg
    14641 caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga agaccagcta
    14701 cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg gccgccgcgc
    14761 caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa
    14821 agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg
    14881 tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca cgaactgtgg
    14941 cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat cctggtggac
    15001 gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc gggcgacacc
    15061 ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg cggggcgcag
    15121 gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat tcgtctggag
    15181 caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat tgaaaacaat
    15241 aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc tatttccctc
    15301 tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat caaaacctgg
    15361 caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa cgcccagtcg
    15421 cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta cggcgggatg
    15481 cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct gattgccaac
    15541 cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg tattggtgac
    15601 cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact ctggcaggca
    15661 tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc cttacagcgg
    15721 tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct gcatgtacag
    15781 actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga gaagggcgaa
    15841 aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg gcagttcagc
    15901 tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca tgcggcgctg
    15961 gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat gaccatgcac
    16021 tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga gggcatgttc
    16081 ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg tctggcctac
    16141 gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac tcgccgtctg
    16201 tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc ggaagagtgt
    16261 gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca gcggatgggt
    16321 acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg ccacgctaag
    16381 tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg tttgcaggtg
    16441 gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct ggagtcggtg
    16501 taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat tcagattcaa
    16561 taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc atcagcagca
    16621 tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga aatcatggtg
    16681 acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg tgaaatcgtg
    16741 ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca ggcaaaatca
    16801 taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt attaatccca
    16861 acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt aattacaccg
    16921 ttttccatca acattggtcc tgactgcacc gcaaactgaa tatctttact ggctttaaaa
    16981 gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc gccaggacgg
    17041 ataaagaaat tcccttcacc tgaagcgaga tttaacgcta ccttc
//
LOCUS       DADLXX010000088        17002 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLXX010000088 DADLXX010000000
VERSION     DADLXX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.089"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNX27_002747"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNX27_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7883902.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="JNX27_002748"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="JNX27_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7883903.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="JNX27_002749"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="JNX27_002749"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7883904.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="JNX27_002750"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="JNX27_002750"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7883905.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="JNX27_002751"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="JNX27_002751"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7883906.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="JNX27_002752"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="JNX27_002752"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY7883907.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="JNX27_002753"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="JNX27_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7883908.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="JNX27_002754"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="JNX27_002754"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7883909.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="JNX27_002755"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="JNX27_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7883910.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="JNX27_002756"
     CDS             7148..7759
                     /locus_tag="JNX27_002756"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7883911.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNX27_002757"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNX27_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7883912.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNX27_002758"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNX27_002758"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY7883913.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNX27_002759"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNX27_002759"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7883914.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="JNX27_002760"
     CDS             complement(11999..12358)
                     /locus_tag="JNX27_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883915.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12355..12907)
                     /locus_tag="JNX27_002761"
                     /pseudo
     CDS             complement(12355..12907)
                     /locus_tag="JNX27_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13008..13607)
                     /locus_tag="JNX27_002762"
     CDS             complement(13008..13607)
                     /locus_tag="JNX27_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883916.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13779..14717)
                     /locus_tag="JNX27_002763"
                     /pseudo
     CDS             complement(13779..14717)
                     /locus_tag="JNX27_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14780..15154)
                     /locus_tag="JNX27_002764"
     CDS             complement(14780..15154)
                     /locus_tag="JNX27_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY7883917.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="JNX27_002765"
     CDS             complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="JNX27_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY7883918.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16326..>17002)
                     /locus_tag="JNX27_002766"
     CDS             complement(16326..>17002)
                     /locus_tag="JNX27_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7883919.1"
                     /translation="GKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAIDCLVFNN
                     ANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIEFKKFIIN
                     NKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFKAKYKRLG
                     LVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKADKIYILLM
                     LILNIF"
BASE COUNT         4920 a         3972 c         3711 g         4399 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcagg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgtgctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcagaata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaaataatac
    12421 acacaaggat aaaatatcgt cccaacttgt aatttgtaat gttgatatct tttattaaac
    12481 ttgcaattga gatcgcaacc agcaatactg acaatgggta aatgggtgat aaatacctgt
    12541 caccttgcgt aacagaaaac caaaataaga catatgttaa aacaatcgag aaaaataccc
    12601 aaagtatttt attattccta agtgtaaaga cagacagtat agcataaaaa acaacatcac
    12661 tgccaacttt attaattgct tgttgcaaat ctaatgtggc aggacttata ccatgtcgag
    12721 cttgttctgc tgtttgaact tgtagatcaa tttcattcca taggtagtga ccaaatatat
    12781 ttcctccagc aggactaaaa ggatcaccaa agatccagaa actacggaca taccaataaa
    12841 tgccaccaag aattgttgcc aagctaaaaa taagaaatgt ctgtatgttt ctctttttcg
    12901 ctatcaatat aacaaatatg atggctccat atatggcacc aaatagtttt acgccacaag
    12961 caattccaat aaaaaaacca cataaaaaaa ttaattcatt ttgtgtttta ttgttttctg
    13021 agcgaatata tataagaata ctggctgcag caaaacaaag atagcgttaa atcaatatat
    13081 gcataaccaa gatgatcttt aagtggattg atagtaaata gtaggattgt agcaataatg
    13141 ctaggataat aagttttcgt aatttcccat gaacagccaa caagtccaca ggctattaca
    13201 aaaaaacaga tatttgctaa aaactgagct aaccttacat ctcctaactg taaacctaaa
    13261 gccattaaaa gatctgcatt ttgcgggaac agaggaaaac gcaaatattg ttcaacaaca
    13321 attgcatggt gctcaactaa tgaacgagcc aaaggtaagt gatacatagt atcatcccaa
    13381 tcaccgggag ctcgcaggga cgataacaat gtaaccacaa aaatcaaaac aagaaataaa
    13441 ataacaaaca gcgtactgaa ctcaatatta tttattgcca gccatctaaa ttgctgacgt
    13501 ttaataatgc ttaaaaccca acacaccgta aaaattaaaa agatagcaat cttattaaat
    13561 tgcccgacca tgcccagaaa gtaaattatc aaaatattta aagacatccc tatgcatagg
    13621 caataaaaaa ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac
    13681 aatacaatat taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac
    13741 aaatacaacg gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt
    13801 ttttcagaaa gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa
    13861 ataatgccac atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca
    13921 gtcggaaatc ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa
    13981 attatagaca aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca
    14041 agcgagtaaa gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt
    14101 ttgctcacac tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata
    14161 cgtaattcta aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat
    14221 ggaaaagatt taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa
    14281 ttccctttaa agatagtctg aacagtacca gtcaacagtt tatttcctca gcgatgccct
    14341 tttctgtatg tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga
    14401 ttttttatga gatggttaat tatttctgga gcagaacttg catcataagt gccatctcca
    14461 tcaaccatta gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc
    14521 ttgcctttca gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta
    14581 ctgtcagtcg aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa
    14641 tcatttatga cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata
    14701 ttaaaagagt gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt
    14761 gtttttaaca aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa
    14821 ccaaactaaa aagagaaaag gtgaacaatg taaatattgg agataaagct aagagatcaa
    14881 ataaaacgcc gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag
    14941 ccttggttgc agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg
    15001 cacatataaa accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg
    15061 cataaaatac cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa
    15121 aagaactggt caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt
    15181 tattactaat ttggatattt tctatagaaa atacccataa aactacaacg cctataataa
    15241 aaattcccat gggtgtaaaa agaaatggat tagttataga aacggtaata ctgaaaaaaa
    15301 gtattaacga catcattaat gatgtttttg tactatttaa aaaatagcct ttcaaaaaga
    15361 taagcaacat aggaactaat gatataaata caccaataac gcctgttttc tgaagtatct
    15421 caagaggtac tatttcaatc cttaatcgat ctaatataaa tgaaccaaat cctcttccaa
    15481 acaagaacgt cgctaaatca acatttttat aataaaaata taaatcatta aatctaacag
    15541 agtcagaatc ttcactgcgt gatccgacca tgtaaagcat aattataaat agaacgctgc
    15601 ccaatattat tataatagta tttaaatata gcttatgtaa tttaaaactg gctaataaca
    15661 gtgataatgt tgtaaaaagt aatacacctc ttgattctgt caataatatc gcaagcacta
    15721 ttatgagaca ttttaaatat tttttgtcga caaaactgat aatcgcaccg acaccaaaaa
    15781 agtagaagga tttggaaaaa aaagccccat cgggacgaaa aaagaactcg ctagtcaatg
    15841 ataagtgttc ataaattaaa ttaaaattta acaaaccgaa attcaacaaa attatgtatg
    15901 aaatatataa cagtgatcca tataatgcaa atgcattaac tattttaata aaaatctcac
    15961 ataaaaatac ttttaaagta tatcttagtg taaatagata tataagtgac agcataaaaa
    16021 ggtatggttg cacatcaaac agtatatcat ttattttatt accagctaat aagccgacaa
    16081 agaagccata aaaaggaaat aaaagcaacc atataaaaag actgacacat ttttgggtga
    16141 ttgaaaaatt gaatgtaact aaattaaaaa ttaaaagtat aaaactaaat acaaataata
    16201 aatatcgcaa agggaatatt cccaatggct ccagtaatct tccaccacca ccaataatca
    16261 gttcaataca taaaaatgtt ataaaaattt tatttatatt attcatattc gtaaggtgat
    16321 gttttttaaa atatgtttaa aatcaacatt aataaaatat aaattttatc agcctttttc
    16381 cgccttaact tccaaaaact taaagccaag atcaaattgc ctatataacc tttacccaat
    16441 tgattattca caaagctatc atcagctaag gcattagcta ttttagttac ttcttttcga
    16501 taccacttgt ttctcaccaa accaagccgt ttgtattttg ctttaaaact aatgtttgct
    16561 cccacttggt tgttttcatg ctgtctgtac agcattgttg gttttctatc aatataccac
    16621 gaataatttc tcgtccgcgc aaatgaatat aaaaaccaat catgcagaca gatatcttgt
    16681 gcagcatttt tattatttat gatgaacttc ttaaattcaa taagcgtttc tttttttata
    16741 acatatgtac accccggacc tgctgcttca aaaaaataat cgaattgagt ttgcgactga
    16801 gccttatcaa ctaaattttt ttgccctgat ggataatatg caattacatt gctggaataa
    16861 caattcgcat tattaaatac caagcagtca attgcatttt ttattttatc tttataccaa
    16921 atatcgtcct gatcactaaa acagatataa tcatagtttt caagatcgac atctttaatt
    16981 aatctataaa aatttttacc tg
//
LOCUS       DADLXX010000089        16902 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLXX010000089 DADLXX010000000
VERSION     DADLXX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.090"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..882)
                     /locus_tag="JNX27_002767"
     CDS             complement(<1..882)
                     /locus_tag="JNX27_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7883920.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="JNX27_002768"
     CDS             989..1285
                     /locus_tag="JNX27_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7883921.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNX27_002769"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNX27_002769"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7883922.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNX27_002770"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNX27_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7883923.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNX27_002771"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNX27_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7883924.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /locus_tag="JNX27_002772"
     CDS             4297..4380
                     /locus_tag="JNX27_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7883925.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNX27_002773"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNX27_002773"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7883926.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /locus_tag="JNX27_002774"
     CDS             complement(5858..6700)
                     /locus_tag="JNX27_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7883927.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="JNX27_002775"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="JNX27_002775"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7883928.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADILAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNX27_002776"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNX27_002776"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7883929.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNX27_002777"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNX27_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNX27_002778"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNX27_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7883930.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="JNX27_002779"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="JNX27_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7883931.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNX27_002780"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNX27_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7883932.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="JNX27_002781"
     CDS             14491..15690
                     /locus_tag="JNX27_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7883933.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4301 a         4219 c         4363 g         4019 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacatac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctgacc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctgactac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaaa tttaaaatca ccccctctgg attgaatcaa
    15961 cttgctctat ttaagacgat tgtttttaca ttttcaccac ctgtatcagg tggtgttttt
    16021 ttattggcgg caatgccttt atcttatttt tattacgttg taaatatggc tattcctaaa
    16081 tgaaatttga tttgctcagt ttatttgtga tgtcagggga gtgcttgcca tgagagtcgt
    16141 gatattatta ttcatcattt ttataccgag tgtggcggat gctttttcat tcgatagttc
    16201 aataattaat ctcaaatgtc ctaggcgtgg aactatcgaa ataatattac acagatacga
    16261 acacacccag gaaagctggg gaggggagaa ttttgaaacc gggagtgggt atagtcatga
    16321 gggcgcgtta tttatttttg actttgccaa tcaggataaa atgatttaca atcgcgcgga
    16381 gaattcgttt gctttttggt atgaatcttc ccggaaatta gttaactgta acttaataac
    16441 gcttaaaagc acatatcctg tcaaccttcc ttatgttcat ggctgatgtg atgtgatgtg
    16501 atgtgtgtcg ggaactaaca tcttcatctt tgtatatgac attgcgataa tgttaagctg
    16561 gtctttgtca taaatttacc cttagagata ctactccctt ctgtcaatat tattcaagcc
    16621 gaacgttata ctggcttttg tcggtcacgg atatcttttt ttcagtgaga attcaaagtg
    16681 agggtatata agaagtcgta tgagtaagta tctttgttgt ggtgggttat tattttatgg
    16741 gcaaaatatt aagcagcgaa atcttttttt tgcccagacg ttttttgtag aaaaaagaat
    16801 ttattagtcc ggctgaaaag caagaactgc tataactaat taagcgtaac ggaacggtac
    16861 aaaacatgcc cttacaacaa ttttcccgat tagcgctcac tc
//
LOCUS       DADLXX010000090        16758 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLXX010000090 DADLXX010000000
VERSION     DADLXX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.091"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            191..2045
                     /gene="ldtD"
                     /locus_tag="JNX27_002782"
                     /pseudo
     CDS             191..2045
                     /gene="ldtD"
                     /locus_tag="JNX27_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            2226..2774
                     /locus_tag="JNX27_002783"
     CDS             2226..2774
                     /locus_tag="JNX27_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7883934.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2801..3448
                     /gene="gloC"
                     /locus_tag="JNX27_002784"
     CDS             2801..3448
                     /gene="gloC"
                     /locus_tag="JNX27_002784"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7883935.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3671..4861)
                     /locus_tag="JNX27_002785"
     CDS             complement(3671..4861)
                     /locus_tag="JNX27_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7883936.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="JNX27_002786"
     CDS             complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="JNX27_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7883937.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="JNX27_002787"
     CDS             complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="JNX27_002787"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7883938.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="JNX27_002788"
     CDS             complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="JNX27_002788"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7883939.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9773..12385
                     /gene="pepN"
                     /locus_tag="JNX27_002789"
     CDS             9773..12385
                     /gene="pepN"
                     /locus_tag="JNX27_002789"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7883940.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="JNX27_002790"
                     /pseudo
     CDS             complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="JNX27_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13356..14003)
                     /locus_tag="JNX27_002791"
                     /pseudo
     CDS             complement(13356..14003)
                     /locus_tag="JNX27_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="JNX27_002792"
     CDS             complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="JNX27_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY7883941.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15157..16116)
                     /locus_tag="JNX27_002793"
     CDS             complement(15157..16116)
                     /locus_tag="JNX27_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7883942.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="JNX27_002794"
     CDS             complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="JNX27_002794"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7883943.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4186 a         4319 c         4289 g         3964 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga tgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cacaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg caggatggaa tgacaaacgt atttctgatg
     1861 ctctgaagca gggtgataca cgttacgtca atattcggca gtcgattccg gtgaatctct
     1921 actacctgac ggcctttgtt ggtgcagatg atcgtaccca gtatcgtaca gatatttaca
     1981 attatgatct gcctgcgcga tccagctcgc aaatcgtatc gaaagcggaa caattaatca
     2041 ggtaaatgaa gtagttcggg gaaatcaatt gtcgtaataa ttagcatgat tgggggcgat
     2101 tctctgcagc ccccgtcact gctggggttg agtcatcttg acgtctgctt tacgggcggt
     2161 taaggtgcct cttgtgcgcc agaagtgcat ataaacgata acattgacct gtagacttga
     2221 ttatcatgga caaattcgac gctaatcgcc gcaaattgct ggcgcttggt ggcgttgcac
     2281 tcggtgccgc catcctgccg acccctgcgt ttgcaacact ctctacccca cgcccgcgca
     2341 ttttgacact caataatctt cataccggag agtcaatcaa agcggagttt ttcgatggca
     2401 gaggctatat tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat taccgcgcga
     2461 acaaaataaa gtccatcgac ccaggattat tcgaccagtt gtatcgcctg caagggttgt
     2521 taggcacgcg caaaccggtg caactcattt ccggttatcg ttccattgat accaacaatg
     2581 aactacgcgc ccgcagccgt ggagtagcga agaaaagcta tcacactaaa ggccaggcga
     2641 tggatttcca tattgaaggt atcgcgttaa gcaatattcg caaagccgcg ttatctatgc
     2701 gcgcaggtgg tgtaggatat tacccacgta gtaactttgt gcatattgat accgggccag
     2761 cacggcactg gtagtaatcg cttaacgaaa caggggcagt atgaactatc gtattattcc
     2821 ggtcaccgca ttctcccaga actgttcatt gatctggtgt gaacaaaccc gcctggccgc
     2881 actggtcgat cctggcggcg atgcggaaaa aatcaaacag gaagttgatg acagcggcct
     2941 gacactgatg cagatcctgc tgacgcatgg tcatctggac cacgttggcg cagcggcgga
     3001 actggcgcaa cattacggcg tgccggtttt tggcccggaa aaagaagatg agttctggct
     3061 gcaaggcttg cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagt cgctgacgcc
     3121 agatcgttgg ctgaacgaag gcgataccat cagcataggg aatgtgactt tacaggtgtt
     3181 acattgccct gggcatacgc cgggtcatgt cgtgtttttt gatgatcggg caaagctgct
     3241 gatttctggc gatgttattt tcaaaggcgg agtagggcgc agtgacttcc cgcgtggcga
     3301 tcataatcaa ctgatttctt caatcaaaga taaattgctg cctctggggg atgacgtgac
     3361 atttattccg ggtcacggac cattatctac acttggttat gagcgcctgc acaatccctt
     3421 cctgcaagac gaaatgcccg tctggtaaag gcgcataaaa aagtccactt ttaatgcggg
     3481 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
     3541 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag
     3601 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
     3661 tttttaacgc ttacagcact gccacaatcg attcgcacag cggagccatg ttatctggtg
     3721 tcatcccggc cacatttacg cgaccagaag caacagcata tacgccaaac tcttcgcgca
     3781 gacgcagcac ttgttctttt gtcagaccac tgaaggagaa catgccgttc tgtttgatga
     3841 taaagctgaa gtcgcggttt gcaccttttt cctgcagcgt attgacgaac aactgacgca
     3901 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaatgcat
     3961 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggagggtta gagtagttag
     4021 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac gatttcactg tcggcagcaa
     4081 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
     4141 caacaatcag ctctttatgc atagccgaga aagcgcgcag tccttcagca tcttcttcca
     4201 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg
     4261 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
     4321 agcagccatg gaacagcact acgtcgccag cctgagcttc gttcagactg ttaatcagtg
     4381 catcgaagtc aagggtgtga ttttccgcat cataataagc gtattcacga acttccagac
     4441 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacacacgct
     4501 taacgctggt attttttgcc aggaagtcgg cagccacgcg tagtgcgcca gtgccacccg
     4561 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
     4621 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
     4681 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
     4741 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
     4801 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
     4861 tgacgaggtt ccattatggt tatagaaggg aagtccgcta tcagggtaac gggagattta
     4921 caaaattcca attattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
     4981 tggaaggcat aaaaaaaaca ggaccaacgt cctgtttttt cggcatttaa caaagaggtg
     5041 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
     5101 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
     5161 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
     5221 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
     5281 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
     5341 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
     5401 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
     5461 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
     5521 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
     5581 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
     5641 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
     5701 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
     5761 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
     5821 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
     5881 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
     5941 agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa
     6001 aaataatgca gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag
     6061 atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata
     6121 ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt
     6181 caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt
     6241 gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat
     6301 aaagatttag tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggagctttgt
     6361 gaacaccgtc atatttccat agaaacgtga tgatatttac agcaattttt atctatttat
     6421 atgatttact tatatttaaa ttaactaaac ggaaattttg tttctgatgg aaactttatc
     6481 gacctggcac aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt
     6541 gctatcagct aagaaaaaac gaatccgcaa taagaatgcg ggttattttt tgtgcgtaaa
     6601 tgttgcaatg taatattccg taacaggatg atcgttatgg cgggggtgca aatagattgc
     6661 ttgacttaac aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct
     6721 ctttgaggaa gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc
     6781 acgcacgttt tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc
     6841 tgaatgcgga acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt
     6901 caggcccatt tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc
     6961 accaatgatc tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc
     7021 ttcgttgagg cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg
     7081 tgctttaaag tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca
     7141 gtaaaccggg ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta
     7201 atccacctgt gcgaaatcgg cttcaatgaa gcgttccaga cgtgaaacgg catctttatc
     7261 tacgcgttca gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac
     7321 atatttcagc atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc
     7381 cggctccagc atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa
     7441 agtcgggccg aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg
     7501 gccagatacg gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc
     7561 ctgatcgtta cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc
     7621 accttcggta tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt
     7681 aaagaagcga tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag
     7741 gtttgtgcgc ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc
     7801 cgccatcggg taagtgtctg gatcttcaac ccaaccagca acttcaacct tgctggcctg
     7861 aatttcaaat tgttgcccct ggcccggcga tgccacgact ttacccgtca caatgaccga
     7921 gcagccggtg gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac
     7981 agcctgtaca ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt
     8041 tgaatctcgg cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg
     8101 gccctggagt acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg
     8161 aaaaaataaa cacttgtcca cccgtaaatg ggggggatac ctatgttacc tggcatctgc
     8221 aatcagacaa gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaagg
     8281 gagccgatta gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc
     8341 tttgcgcagc gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga
     8401 aagtttcgcc accggtttac cgttacactc taccaactta atcacaatat tcaggggttt
     8461 tacctggggg atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga
     8521 agagaagtgg cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag
     8581 cgttttactc tgtggatcaa ttcccagctt ttcataatgt gcaatggctt tttcacccca
     8641 ttcaaccggg tcgccagagt catgacgcag gccctgatac cgactagcga actcgacacc
     8701 gaaatcacgc aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc
     8761 gggatactct tccagccagg cagcaagtgc agctcgctgg ctgttggcta gatccgggct
     8821 gatttgctga tgtgcctgga accattcgtg tgcctgtgtt cccatcggcg tgagggaaag
     8881 ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt
     8941 aacgatggtt tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg
     9001 gaagcgcgac atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag
     9061 cgtgtcgagg gcttgtgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact
     9121 gataaccgcc agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg
     9181 aatatccagc ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaactc
     9241 gcgtaaccag ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc
     9301 atcatcctgc aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata
     9361 aatacccagc agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata
     9421 gtgatgaaac acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg
     9481 cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag cgtcctcgta caggagcgtt
     9541 tagtacaata aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac
     9601 cccgatcaca ccatatgcca cctttctgga cgatggcatt acgcggtgta tgttataaaa
     9661 atgtagcaat aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg
     9721 caacaggaat agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca
     9781 acagccacaa gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat
     9841 tgacttgacc tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt
     9901 ccgtcatggt gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc
     9961 tgttcatatt aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat
    10021 cagtaatttg ccggagcgtt ttacgcttaa gatcattaat gaaataagcc cggcggcgaa
    10081 tacggcgctg gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga
    10141 aggtttccgc catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac
    10201 caaaattatt gccgataaaa tcaaatatcc cttcctgctt tccaatggta accgcgttgc
    10261 gcaaggcgaa ctcgaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc
    10321 gtgctacctg tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata cctttaccac
    10381 gcgttctggt cgcgaagtag cactggagct gtacgtcgat cgcggcaacc ttgatcgcgc
    10441 gccgtgggcg atgacctcgc tgaaaaactc catgaaatgg gatgaagaac gctttggcct
    10501 ggagtatgac ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat
    10561 ggagaataag ggtctgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc
    10621 caccgacaaa gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg
    10681 gaccggtaac cgagtgacct gccgcgactg gttccagctc agcctgaaag aaggtttaac
    10741 cgtcttccgc gatcaggaat tcagctctga ccttggttcc cgcgcagtta accgcatcaa
    10801 taatgtgcgc accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc
    10861 aatccgcccg gacatggtca ttgagatgaa caacttctac accctgaccg tttacgagaa
    10921 gggcgcggaa gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg
    10981 gatgcaactc tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg actttgtgca
    11041 ggcgatggaa gatgcgtcga atgttgatct ctcccatttc cgccgttggt acagccagtc
    11101 cggtacgccg gttgtgaccg tcaaagacga ctacaatccg gaaactgagc agtacaccct
    11161 gaccatcagc cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat
    11221 tccgtttgcc atcgaattgt atgacaacga aggcaaagtg atcccgttgc agaaaggcgg
    11281 tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa
    11341 tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga
    11401 atataagtgg agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc
    11461 ccgttgggat gcggcgcaaa gtttgctggc aacctacatc aagctgaacg tcgcccgtca
    11521 tcagcaaggg cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcggtgct
    11581 gctcgatgag aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa
    11641 tgaaatggct gaactgttcg atatcataga cccgattgct attgccgaag tacgcgaagc
    11701 actcactcgt actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa
    11761 ttaccagagc gagtaccgtg ttgagcatga agatattgca aaacgcactc tgcgtaatgc
    11821 ctgcctgcgc ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca
    11881 ataccatgaa gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg cggttgctgc
    11941 acagctgcct tgccgtgacg cgctgatgca ggagtacgac gataagtggc atcaggacgg
    12001 tctggtgatg gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga
    12061 gacggtgcgt ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg
    12121 ttcgttgatt ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg aagatggcag
    12181 cggttaccag ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtagc
    12241 ttcacgtctg attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa
    12301 aatgcgcgcg gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctttacga
    12361 gaagataact aaagcactgg cttgatactt ttgagccaaa atgcctgatg cgacgcttga
    12421 cgcgtcttat caggcctaca actccgttga aattgctggt tttgtaggct ggataaggcg
    12481 ttcacgccgc atccggcagc cgtgcccaac tgcctgatgc gatgcttgac gtgtcttatc
    12541 aggcctacaa gtccgttgaa aatgctggtt ttgtgcgcca catccggcat attaaccatg
    12601 acgccgaatc agttgttcag aatgcccacg ccgcattacc cgttgcaata cttccgcttc
    12661 cagctccgct aatcgcaccg accctaatcg acgcggacgg gggatatcca ctgtcaaatc
    12721 cagactaatt tttccctctt caattaacaa cacccggtca gccattgcta ccgcttcgct
    12781 cacatcatgc gtcaccaaca gtacggtaaa gccgtgctgc tgccaaagtg acacaatcaa
    12841 atcctgcatc tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat caaacaacaa
    12901 taaacccggt cgatgaatca acgctcttgc cagcgccaca cgctgtttct gcccgccaga
    12961 aagtgcggca ggccattccc cggcgcgatt ctccagtcct accgcagcca acgcccgacg
    13021 cgcggcatcg cgccactgac ctttaaggcc taacccaacg ttatcaatca ccgatttcta
    13081 cggcagcaga cgcgcatcct gaaacatcat tcgcgtatca tcctgaattt cagccagcgg
    13141 tgtggtgccc gctaacacat cgcctacggt tggcgtttcc agaccggcca gcaggcgcag
    13201 cagggtactt ttaccaccac cgctgcggcc caccaccgcc acaaactgac ctgccggaat
    13261 atgtaaatcc agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag
    13321 caacaatggt gtgccctggt tcagacgagc agtattcata tcgtggcctc cttcaaatga
    13381 taagccgggt tccagctcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt
    13441 ttgccgagca gggcgtaaag aataatagcg actaccacca cgtctgtttg caaaaactct
    13501 cgcgcattca tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc
    13561 agcgtcagcc acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca
    13621 tcgggcagga tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc
    13681 tccaccagcc cgcgatcgat attacggatg ccatgccagg tgttgatata aatggggaac
    13741 agcgtaccga gcgccaccag aaagattttc gcggattcat caatgccaaa ccacaaaatc
    13801 accagcggga tcagcgccag atgctgccac agttcgccgc tggcggagag cgtccagaac
    13861 gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg acagccagcc aaccgaggag
    13921 gccagttgcc acaccgccac gatgcccacc ggtaaaaacc agggggcaac gcgcaataac
    13981 tacttcttca ctggcgttgc catcatcgtg ttccttagct ttgcgcgact ttacggggga
    14041 taaaatcatt cgccaccgct tcgccttgcg gattcagcgg ctgcggctgg ggaatttccg
    14101 gaatggcgac atccagatgt gggaacagca actcgccaac ccgatacgct tcttccagat
    14161 gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc ggcgtattcg ttgattcgcg
    14221 cggcgaccgt aggaccatcg cccaccagcg ccgttccggc accgccgcgc actaagccaa
    14281 cgcccgccca taaattgggg ctgatctcca gattgtcgcg cttgccgtta tgtagcgccg
    14341 ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc ggcttgtgct ttggcgatag
    14401 tctcatcatc aagatgcgag attaaccgct cggcggcctg ccacgcttcg tcgttagttt
    14461 cacgaacaat cacatgcaga cgaataccga aacgaatttt gcgcccatac gcggcagctt
    14521 tcgcccgcac ttgttcgatt ttctctttaa caagttccgg cggttcgccc caggtgaggt
    14581 agagatcaac ttgttctgcc gccagctcct gggcgacatc tgacgatccg ccaaagtaaa
    14641 gcggaggata aggctgttga atcggcggga agagcagttt tgctccgcgc acatgaatat
    14701 gtttaccgtt gaaattgacg gtttccccca gcaataagcg ccgccagacc tgggtaaatt
    14761 ccgccgaggc ttcgtagtgc tcgctatgat caaggaacac gccgtcgcct gccagctctt
    14821 gtggatcgct gcctgtgacc agattaaaca acgcacgacc atttgagaga cggtcaagcg
    14881 tggcggcctg gcgggcggca acagtaggtg aggttacgct gggacgcagg gcgacaagaa
    14941 acttcagccg ctgcgtcacc gggatcatcg acgctgcaac cagccacgca tcttcacacg
    15001 atcgccccgt tgggatcagc acaccggtat agcccagacg atccgccgct tgcgcaattt
    15061 gttgcagata accgtgatca accgggcgtg aaccttcttc cgttcccaga tagtgcccgt
    15121 caccgtgggt cggtaaaaac cagaacatat tcagactcat aattgttttc cttccagttg
    15181 agtgggctgc cagatgcgct ggcgaatatc gacttttttc ggcaccagac ggttttcata
    15241 aaacagatct gccgtttgct gctgtaaggc ggcaacctcg gcgttaaccg gtttgatggt
    15301 ggtaggaggg cgatgatcta agtaagaggc aatcaccggt gccggtaagc ccatcgtttt
    15361 tgccagtaaa gcgatgcttt gttcgcgctg gctgcgggtt aacgcatcgg cctcactaaa
    15421 ggttgccagt acgccctgaa taaaagcgcc gtttttttct gcatagggac gagctgccag
    15481 ataaaacgat ccagtttgat tgagatcggt gccgtctttc agcacccgca cgccgccctg
    15541 taataacgca gcggagtagt agggatccca gatagcccag gcgtcaacgt taccttgctg
    15601 gaacgcggcg cgggcaccgg caggcgtcag gtaagtgggc tggatatcgg tgaatttaag
    15661 tccggcctga cgcagtgcac gcagtaaaag gttgtgtgaa ctggaacctt tctgaaaggc
    15721 aactttgtga cctttaagat cggctacggt tttgatcggg ctgttttctg ccaccagaat
    15781 cacttcggct ttgggcttcg gtggctcgac gcccacatat accaaatcag ccccggcagc
    15841 ctgggcaaag attggcggaa tatccccggt actgccgaga tcaatactgc caacgtttaa
    15901 cgcttccaac atttgcggac ccgcggggaa ctccacccag gagatttttg attgcggata
    15961 gcgtttttcc agtaactggt ggctttttgc cagcaccata ccaatactgc ctttctgata
    16021 gcctatacgt aacgcttcag gcgaggattc tgcagcaacc gcaaaagtag agacgctaag
    16081 caatcccgcc agcgccagtt taatgatgtt acgcatgggc attacctcgc agagacagaa
    16141 ggtcaggaac ctgaacatcg cggcggtgca atgcctgcca gaaagtttct agcgcggtat
    16201 caagatgggg ttgcagattt ggcgtgaact ggggtctgtg atggtaatca attaattgtg
    16261 agtcatcggc aaacacgccg tgcaggatct cctgagcttt cagtgcgctt aaaactggtt
    16321 taagggcata atcgaccgcc agcagatggg ccacggtacc gcccgtcgcc agcggtagca
    16381 ccactttgcc ttgcaaagcg cgctctggca gcaggtcgag cagggttttc agcgcgccgg
    16441 aataggcggc tttatacaca ggcgtggcga caatcagccc atcagcctgt tgcagctgtt
    16501 cggtgaaggt cttgagtgcc ggactatcga aacgagcata aagcagatct tccggggcga
    16561 agttttgcag attccagtga taaacctcta catctaggcc atttagtttt tcccgcgcat
    16621 attccagcaa ggagctggag cgagaaggaa agcgaggact acccgccagg gtgatgacac
    16681 gcatactctc tccttataac caattgttct tttttttgtt aacattgata acaattcggt
    16741 cagtctgtcg gagagaca
//
LOCUS       DADLXX010000091        16507 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLXX010000091 DADLXX010000000
VERSION     DADLXX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.092"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(283..987)
                     /locus_tag="JNX27_002795"
     CDS             complement(283..987)
                     /locus_tag="JNX27_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7883944.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNX27_002796"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNX27_002796"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7883945.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNX27_002797"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNX27_002797"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7883946.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNX27_002798"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNX27_002798"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7883947.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNX27_002799"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNX27_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7883948.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNX27_002800"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNX27_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7883949.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="JNX27_002801"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="JNX27_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY7883950.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNX27_002802"
     CDS             complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNX27_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY7883951.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNX27_002803"
     CDS             complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNX27_002803"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7883952.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNX27_002804"
     CDS             complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNX27_002804"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7883953.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNX27_002805"
     CDS             complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNX27_002805"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7883954.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIACFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNX27_002806"
     CDS             complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNX27_002806"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7883955.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11614..12903
                     /gene="bioA"
                     /locus_tag="JNX27_002807"
     CDS             11614..12903
                     /gene="bioA"
                     /locus_tag="JNX27_002807"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7883956.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12881..13351
                     /locus_tag="JNX27_002808"
     CDS             12881..13351
                     /locus_tag="JNX27_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7883957.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13666..13749
                     /locus_tag="JNX27_002809"
                     /pseudo
     CDS             13666..13749
                     /locus_tag="JNX27_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            13905..14906
                     /locus_tag="JNX27_002810"
     CDS             13905..14906
                     /locus_tag="JNX27_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7883958.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14940..16496)
                     /locus_tag="JNX27_002811"
     CDS             complement(14940..16496)
                     /locus_tag="JNX27_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HAY7883959.1"
                     /translation="MLKYDPDQFGPDLIEQLAQSGKYSQDNTKGDAMIGVKQPLPKAV
                     LRTQHDKNKEAISILDFGVIDDGVTDNYQAIQNAIDAVASLPSGGELFIPASNQAVGY
                     IVGSTLLIPGGVNIRGVGKASQLRAKSGLTGSVLRLSYDSDTIGRYLRNIRVTGNNTC
                     NGIDTNITAEDSVIRQVYGWVFDNVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHF
                     LGQCVSVSVSSCHFSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFK
                     NAVYVHDCLDLHMEQPDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFR
                     TPTSTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDI
                     NECVSFDKCIFNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGI
                     INVPVAVGATSGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVA
                     LPSMVEYLAI"
BASE COUNT         3938 a         4341 c         4083 g         4145 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttccgtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atctaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
     6241 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6301 cgccaatttg tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca
     6361 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6421 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
     6481 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
     6541 cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
     6601 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6661 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
     6721 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
     6781 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
     6841 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6901 ccagaccata aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg
     6961 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
     7021 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
     7081 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
     7141 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     7201 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     7261 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
     7321 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7381 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7441 atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
     7501 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7561 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat
     7621 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
     7681 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7741 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
     7801 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
     7861 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagaa
     7921 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
     7981 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     8041 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
     8101 tccggcgtgt tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     8161 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
     8221 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     8281 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc
     8341 gctgatgatg tgcggcgagg agggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8401 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
     8461 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
     8521 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
     8581 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8641 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8701 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
     8761 cactgagcgc atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc
     8821 cgttcagcga ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8881 ccaccgcctg ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8941 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     9001 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     9061 gggattcgat atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa
     9121 gcattggcgg cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc
     9181 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca
     9241 ttgccagtaa ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg
     9301 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9361 agcagacgat cgatatcctg catttcatgc gcagcggtta gcgttaagcg cagtcgcgca
     9421 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
     9481 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9541 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaaca cgcaatgagt
     9601 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca
     9661 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
     9721 tgcagcagat aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat
     9781 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
     9841 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
     9901 tgctgttgcg ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac
     9961 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    10021 tgagcgacat cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    10081 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
    10141 gcaatgactg cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca
    10201 agccactcgg ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga
    10261 ccggagccgc cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt
    10321 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10381 tccgccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10441 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    10501 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt
    10561 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10621 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10681 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10741 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa
    10801 aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    10861 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    10921 cgcgatcttt taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg
    10981 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat
    11041 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg
    11101 cgaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    11161 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    11221 tccatgccgc gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact
    11281 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11341 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc
    11401 tgacctgcac ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca
    11461 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11521 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac
    11581 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11641 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    11701 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11761 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    11821 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    11881 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11941 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    12001 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    12061 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    12121 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc
    12181 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    12241 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12301 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12361 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    12421 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg
    12481 cgcgaggttg cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12541 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    12601 ggcgaatggc agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca
    12661 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    12721 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg
    12781 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12841 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    12901 taatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa
    12961 tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac
    13021 ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt
    13081 acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga
    13141 cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc
    13201 gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga
    13261 tgtcgatgaa ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc
    13321 aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct
    13381 ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa
    13441 gttagtataa aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa
    13501 agatttagtc taaataatag actgcataat actacaaaac acaacatatc cagtcactat
    13561 gaatcaacta cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt
    13621 ttgtcctctt gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct
    13681 aagtatctcg ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag
    13741 gttaggtagg caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat
    13801 tctttggcct caacgcgagc atgacctcac tggctggacc caaaatggac tatgacagaa
    13861 agatctgata tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact
    13921 gctttgacac ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc
    13981 tctctgggca gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta
    14041 tcatattctt ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct
    14101 ttattgattt catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta
    14161 tactgacata ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc
    14221 atgacattgt caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca
    14281 ttacatcaca tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt
    14341 tcctgtgtta tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca
    14401 tagttatact gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg
    14461 atgtaatgtt ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg
    14521 gtgccactat ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag
    14581 tttctttgct tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga
    14641 cttgctatat cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac
    14701 tggttaaggg caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc
    14761 aggttgttat aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga
    14821 ctgttctttt cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa
    14881 gttcgcctaa attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat
    14941 cagatggcca gatactcaac cattgaaggc agcgcgactc ctactgggct tgggcgagta
    15001 acattaattg tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa
    15061 aggcttctta ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg
    15121 gcacctaccg caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca
    15181 cgatacgtat tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag
    15241 tcagtaactg acacgccacc gctacgaaga tagcatagag gcttattgaa tatgcactta
    15301 tcgaaagaaa cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac
    15361 gtacaaccac ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt
    15421 gcagccgtgt ttttgtttgc agtgttgata tgaacaccac tgacaatttt atgtgactgc
    15481 gttgaggtcg gcgttctaaa atatatcccc gtaaattgtt cagtgccatc ggcatctgct
    15541 gctatccatg agtttgagaa agaaaatcct ccgtttacat tctctataac cacgcctgtt
    15601 gaaccgcaat aatctaaatc cggttgttcc atatgcaaat caaggcaatc atgaacatag
    15661 acggcatttt taaaaccaat gcacatggtc tcactgtcta aaattattgc ttctgaccta
    15721 actgcctctg acgaccacgc atacgtttga ggctgaatcc tgatgccaaa gctttcatca
    15781 gcagaataat ttcctctgct gaaatggcag gagctgacac taacacttac gcactggcct
    15841 aaaaagtgaa gaccgactct acaggttcca gcctgacatg ctataaattt tgagtgccag
    15901 agcccttgca ttaaataagc ggtttcaact tcattcacca ttacattatc aaatacccag
    15961 ccataaacct gtctgatgac agagtcttct gctgtaatgt ttgtgtcaat accattgcag
    16021 gtgttattac cagttactcg tatatttctc agataacggc cgatagtgtc tgaatcataa
    16081 gacagcctta acacagatcc tgtaagtccg ctttttgctc ggagttgcga tgccttacca
    16141 acccctctga tgttaacacc gccaggaata agcaaagtgg atccaacaat ataccccacc
    16201 gcttggttgc tcgcagggat aaacagctcc ccgccggagg gtagtgaagc aacggcatct
    16261 attgcatttt gtattgcctg gtaattatct gtcacaccat catcaataac accaaaatcc
    16321 aggatactta ttgcttcttt atttttgtca tgctgagttc ttaaaactgc ttttggtaaa
    16381 ggctgcttga cgccaatcat ggcatcgcct ttggtgttat cctgcgaata cttaccggat
    16441 tgagctagtt gctcaattaa atccggaccg aactgatcag ggtcatactt cagcaaattc
    16501 ggaaaat
//
LOCUS       DADLXX010000092        16367 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLXX010000092 DADLXX010000000
VERSION     DADLXX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.093"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            132..839
                     /locus_tag="JNX27_002812"
     CDS             132..839
                     /locus_tag="JNX27_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7883960.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            836..2287
                     /gene="djlC"
                     /locus_tag="JNX27_002813"
     CDS             836..2287
                     /gene="djlC"
                     /locus_tag="JNX27_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY7883961.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIINRMLLAKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2347..4017)
                     /locus_tag="JNX27_002814"
     CDS             complement(2347..4017)
                     /locus_tag="JNX27_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY7883962.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASASDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4101..5036)
                     /gene="rihA"
                     /locus_tag="JNX27_002815"
     CDS             complement(4101..5036)
                     /gene="rihA"
                     /locus_tag="JNX27_002815"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7883963.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(5154..5879)
                     /gene="gltL"
                     /locus_tag="JNX27_002816"
     CDS             complement(5154..5879)
                     /gene="gltL"
                     /locus_tag="JNX27_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7883964.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5879..6553)
                     /gene="gltK"
                     /locus_tag="JNX27_002817"
     CDS             complement(5879..6553)
                     /gene="gltK"
                     /locus_tag="JNX27_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7883965.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6553..7293)
                     /gene="gltJ"
                     /locus_tag="JNX27_002818"
     CDS             complement(6553..7293)
                     /gene="gltJ"
                     /locus_tag="JNX27_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY7883966.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7463..8370)
                     /gene="gltI"
                     /locus_tag="JNX27_002819"
                     /pseudo
     CDS             complement(7463..8370)
                     /gene="gltI"
                     /locus_tag="JNX27_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            complement(8767..10305)
                     /gene="lnt"
                     /locus_tag="JNX27_002820"
     CDS             complement(8767..10305)
                     /gene="lnt"
                     /locus_tag="JNX27_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7883967.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10330..11208)
                     /gene="corC"
                     /locus_tag="JNX27_002821"
     CDS             complement(10330..11208)
                     /gene="corC"
                     /locus_tag="JNX27_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7883968.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11298..11765)
                     /gene="ybeY"
                     /locus_tag="JNX27_002822"
     CDS             complement(11298..11765)
                     /gene="ybeY"
                     /locus_tag="JNX27_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7883969.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     MVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11762..12841)
                     /locus_tag="JNX27_002823"
     CDS             complement(11762..12841)
                     /locus_tag="JNX27_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7883970.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AREEAEQKRKAALAAERKREEQEQK"
     gene            complement(12955..14379)
                     /gene="miaB"
                     /locus_tag="JNX27_002824"
     CDS             complement(12955..14379)
                     /gene="miaB"
                     /locus_tag="JNX27_002824"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7883971.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14525..15700
                     /gene="ubiF"
                     /locus_tag="JNX27_002825"
     CDS             14525..15700
                     /gene="ubiF"
                     /locus_tag="JNX27_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7883972.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15854..15928)
                     /locus_tag="JNX27_002826"
     tRNA            complement(15854..15928)
                     /locus_tag="JNX27_002826"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15894..15896),aa:Gln,seq:ctg)
     gene            complement(15977..16053)
                     /locus_tag="JNX27_002827"
     tRNA            complement(15977..16053)
                     /locus_tag="JNX27_002827"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16017..16019),aa:Met,seq:cat)
     gene            complement(16069..16143)
                     /locus_tag="JNX27_002828"
     tRNA            complement(16069..16143)
                     /locus_tag="JNX27_002828"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16109..16111),aa:Gln,seq:ttg)
     gene            complement(16178..16252)
                     /locus_tag="JNX27_002829"
     tRNA            complement(16178..16252)
                     /locus_tag="JNX27_002829"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16218..16220),aa:Gln,seq:ttg)
     gene            complement(16276..16360)
                     /locus_tag="JNX27_002830"
     tRNA            complement(16276..16360)
                     /locus_tag="JNX27_002830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16324..16326),aa:Leu,seq:tag)
BASE COUNT         3989 a         4166 c         4238 g         3974 t
ORIGIN      
        1 tgcgaatata tacattaatt ttttattcat gtgtgaatgc ttcgattttc tttttatttt
       61 gaaagcgtca agtttatact catcaataat aaaaaagtag tcagttaatg cacttaaggg
      121 attattaaaa aatgaataag gaagaacaat atcttctttt tgcgctttct gcgcccatgg
      181 aaatacttaa tcagagttgc aaacccgcgc atgactcgcc aaaaatgtat accgggataa
      241 aagaattcga tctctcatca tcctggggaa taaataatcg tgatgattta attcagacta
      301 tttatcagat gactgatgat ggtcatgcca atgatttagc tggcttatat ctgacatggc
      361 aacgctcttc ccctgaagag tggaaagcat taattgctgg tggctcggag agagggttga
      421 tttataccca attcgttgcc caaaccgcta tgtgctgtgg agaaggggga atcaaagcct
      481 gggattatgt tcgaatgggg tttctgtcac gggccggtgt gctcaacaac tggctgacgg
      541 aagaagagag tttatggctg caatcgcgcg tctatgcacg agctcaccat tactatcaca
      601 gctggatgca ctatttttct gcctattcac tagggcgtct ctactggcaa tcttctcagt
      661 gcgaagacaa cgcttcgcta cgcgaggcgc taaccctgta caaatacgat agcgccggga
      721 gtcggatgtt cgaagagctg gcagcgggaa gcgaccgttt ctatgccacg cttccctggc
      781 agccattaac tgtccagcct gaatgcccgg taacgcttaa ggatgtgagc gacctatgaa
      841 aacatgttgg caaatcctcg aaattgaaag cacgacgcaa atagacatta tccgccaggc
      901 ttatcttgct cgcttaccgt tgtgtcatcc cgaaaccgat ccgcaggggt ttaaagcatt
      961 acgccaggcc tatgaagagg cgctgcgact ggcggtaaat cctgtcgagg aagcagatga
     1021 tgaagaaaaa gatgccaccg ctgaacatga aatactacgt gcattcagga cattactgga
     1081 ttcagaaagt gatcgttttc agccctccgc ttggcagaaa tttattcagc aattaaatac
     1141 ctggaacatg gaggatgtcg atcaattacg ctggccgctg tgtgcaatcg ctatagaagc
     1201 gcgatatctg tcattaaatt gtgcttcttt gttggcagag cgtttgaact ggcattcgtt
     1261 taatgacagc gaaggaatgg atgaggaaga aagggaggct tttcttgagg ccattcaggc
     1321 tggtgattgt ttcgatttcc ttagccttct ggaatatccc gtcgcgttac agaaccagac
     1381 tgttgaatat tacttcgcgc tggaacgttg ctgccgttac catcctgact atgtcactgc
     1441 gtttttggcg atggaaggac cgtggttcat tcctgatgat gcaaagttac atcgcaaact
     1501 gttgcgctgg tacagctcgg tgcaaacagg tatggtggaa ctcattcctg tagccaaaca
     1561 gtggcaagcg gaagaaccag aaagcgaaga tgcccggtat taccagtgtg cacaacgttt
     1621 gtactgcggc gagggggaaa gcctgcttgc tgatctcggc gcgtactggg aaagttaccc
     1681 atctacacaa gctgataatt tgttgttgca gtggagcaag cgtcattgcc cggattattt
     1741 cgcgttatta gtgatggtta tcgaagcgcg gagcatggta gatgcgcaag gtcaaccgct
     1801 gaaatatgtt cctggtgaga gcgcccggac gcggctgtta tgggcggaaa ttttacatag
     1861 cggaaaatta tcgccgttag gtcaatcgtt tattgagtcg ttattcttca aacgcaaagc
     1921 atgggcgtgg tggaaatcga gagtcggtag cgagacagaa gaagattcac cgttactgga
     1981 tttgtatcgg gtagcggaac aggtagtact tgaagcgttt ccgaaacaag agatgctggc
     2041 ccgtcttaat acaaggctgg aaggcggaga tgctcatcca ttagaggcca ttatcaaccg
     2101 gatgctgttg gcgaaagtaa aactcgagcc ggaggatgaa gatgtcgatg agccaacacc
     2161 tgaaaaccat gaagaaaaaa atgatgaggg tgaaaaacca cagagcatta ccagcattat
     2221 caaaatcagt ttaacggtgc tggttatagg ttatgctctc ggcaaaatcg cgatgttgtt
     2281 tagttgacga tgtgtttttt gcgccgatgc cggatgcagc atttacgccg catccggcag
     2341 ccgtgattat gaggtctcaa tggctaaata aacgctcgcc cgccgcctga cctcatcgac
     2401 gcgggccata tcgtttgact gcagcgccgc atcaaaatcg gtgatcaccc ggccaatctc
     2461 ttctcgctcg tcacccagcg cccgcgccca caactcttcc agtttggctt taaaggtgcg
     2521 attgatgagc atatcgcgcg gataaatttt caatgcggat aaccgcgtcc gactctcttc
     2581 aatctgctgc gcgctcaatg ttaccgggct gtggttaatc actctggact taacgctgcc
     2641 gtcttccaga agcacgtcaa cttcaagcaa cccgttgata tcataactaa agcgaatatc
     2701 aatcgactga taagccccgg ttttcttcaa cggcacatcg aaggattcca ccagaatgtt
     2761 gtttttaact ttgtggtttt ctccctgata gacgttaacc gtaattgaat cctgttccgg
     2821 gtgcatggtt gaataagttt ctacacgcga cacgggcaca gtggtgtttc gttcaataat
     2881 cggcgagaaa atgccggaaa cgccctggcg gttaacctca acgcccaacg agtaagggca
     2941 aatatcagtg aggatcactt cttcaatatc ttcactgcgt aagcggcagg cggcctggat
     3001 tgctgcgccc agcgcgacaa tggtgctcgg atcgtaactt tgatacggta atttgccaaa
     3061 cagacgcacg gcgattcgct gcaccagcgg catttgtgac gcgccgccaa ccagcaccag
     3121 actgtcgatt tgactcggct tcagacgcgc atcgcgcaac gcctgttcaa tcggcactcg
     3181 caagcgattg agcagcggca accacaaatc ttccagttcg ttctcgtaaa attcgcattc
     3241 ccgcgtttca tcctggtact gccagcgaat gtgcattggc gattgattgc tacattttgc
     3301 cgcttccaca caggcgtaca aggctgccag ttcactctcg ttaagcgtgg tccttgtgac
     3361 atccgcgcgc tttaaaacgt catcgaccaa catatgggta aaatcttcgc caccgaggaa
     3421 gttgtcgcta gcggaggcgt gaacttcaat caccggcgtg gcgtactcaa gcaccgtaac
     3481 gtcaaacgtg ccgccaccga gatcaaacac cagcgaacgg gtattttgtt gggtatgcag
     3541 gccatacgcc atcgcagctg ctgtgggttc attaattaag cgtaccgcat ttaacccggc
     3601 taagtccgct gctaaacggg tatgcttgcg ttgtttatcg ctgaaataag ccggaacgga
     3661 gatcaccaca tctttaatcg gacgttgcag aaattcttcg gcatcttctt ttaatgagcg
     3721 taataccaaa gaggacagtt ctggcgcgtt aaatgtgtcg ctgcctaacc gccagttggt
     3781 attactgccc attgcacgtt taaataacgc tgccgtttta tccggatgcg aagtacgccg
     3841 tgatacagcc ggttttccga ctaaaatatg attattttca tccatgctaa ttatggatgg
     3901 tgttaaatat tcaccgaact tatttggaat taattgcgcg gcaccgtctt tccagacggc
     3961 aattaaacta ttggtagtgc cgagatcgat acctatggcg agttctgcat tatccattgc
     4021 atttatatct tgaaagaaaa agagataaat agattaagac cacccaggta acgcgtggtg
     4081 atcttaatca atgacgagtg ttaagcgtaa aatttcagac gatcagccag taaatcaaca
     4141 aagccctgac gatcaacatc gacaattaca gtggcattcg gtttattgcc agtcagataa
     4201 taataatcaa caaccgtcat acccagggta tatttcccct gtgtttccac gccaacccag
     4261 cgctcaacag tggtaaacaa ctcgggtttc aacagccagg cgatggtgca tgggtcatgc
     4321 agcggtgcgc cgacaaagcc ccatttttcg tctttatgat attcgaggaa gaaatccagc
     4381 agttcggcaa caatggttga aacagggttg ccaatcgcgc ggaaacgctc ggtgtcttca
     4441 acgtggattt gcgctttatg agtaacatcc agaccggcca tcaccaccgg gatccctgac
     4501 tggaagacaa tttctgccgc ttccgggtca acgtaaatat taaattcagc cgcaggcgtc
     4561 cagttaccaa gccccattgc gccgcccata atcacgatac gggcaatttt gctatgcagt
     4621 tccggatggc tattgagcag caaggcaacg ttagtttgcg gtccggtaga aacaatcgtg
     4681 acaggttccg cactttcgcg cagcgttttc gccatcagct ccaccgccgt acagttttgc
     4741 ggtgcgaacg tcggttccgg taatgccggg ccgtcgagac cgctttcgcc gtgcacattg
     4801 tccgcgataa tcaactcacg cattaacggt tttaccgcgc cgcctgccac cggaatatcg
     4861 gtgcgattaa gcaaggtcag catacgcaga acattgcgta aggttttttc tggtgtctgg
     4921 tttccggcgg aagacgtaat tgctttgaca tcaagctctg gtgaggcgag ggcgagaact
     4981 attgcgatag cgtcatcatg acctgggtcg caatctaaca gaattggcag tgccattgtt
     5041 gctccttgtt gtgtgcttct ttgcgacaag ggtaacgcca ggatgtaaca gatacgaggg
     5101 gcgaaacgat aaagcgtgag atggcgcgca attgggtatg cgcgccagag tgattaatgc
     5161 aggattttcg cgaggaagtc ttttgcgcgg tccgatttcg gatcatcgaa gaaagcgtct
     5221 ttcggcgagt cttcgacaat tttaccctcg tccataaaga tcacccgatt cgccactttg
     5281 cgggcaaagc ccatttcgtg ggtcaccacc atcatggtca ttccttcgtt cgccagttcc
     5341 accatcacgt ccagcacttc gttgatcatc tccggatcca gcgccgatgt cggttcgtcg
     5401 aacagcatcg caataggagc catacacaat gcgcgagcga ttgccacacg ctgctgctga
     5461 ccgccggaaa gctgcgccgg aaatttattg gcatgtgcgg aaagtcccac gcgctccagc
     5521 aatttcaatg ctttttcacg agcgggcgct ttatcgcgtt taagcacttt cacctgcgcc
     5581 agggtcaggt tttcgataat cgacagatga gggaacagct cgaaatgctg gaataccatc
     5641 ccgacgcggg agcgcagctt tgccagatcg gttttcttgt cgttaaccac gataccatcg
     5701 acggtgattt caccttgctg cactggttcg aggccgttga cggttttaat cagcgttgat
     5761 ttgccggaac cagacgggcc gcaaaccacc accacttcgc cttttttcac ttcggttgag
     5821 cagtcggtca gcacctgaaa gtgaccatac cattttgaaa catttttcag ggtaatcatt
     5881 atgctgtcct tcttttcaag tagctgacca acaacgacgc gctaaggcta ataacgaaat
     5941 aaacaaatcc ggcaaacagg atcatctcga cctgcgtacc atcacgctca ccaatggttg
     6001 aggcggtacg gaagaaatcg gccagagata acacatacac cagcgaggta tcctggaaca
     6061 gtacgatgcc ctgagtgagc agcagcggca ccatcgcgcg gaaagcctgc ggcagaataa
     6121 tcagtttcat cgactgccag tgagtcatcc ccaacgccag cgcggcgctc gactgaccac
     6181 gggaaatact ttgaataccg gcacggataa tctctgaata gtaggccgct tcaaacatcg
     6241 aaaacgccac catcgccgaa attaaacgaa tatcattttt tggcgataat cccagcacat
     6301 tttgcagaaa tcccggcaca atcaagtaaa accacagcaa aaccatgact aaaggaatcg
     6361 agcggaatac gttaacgtag gctttggcaa accacgaaac aggcgcgaag ctggacaagc
     6421 gcatcaccgc cagcatggta ccccacaaaa taccaatcac gactgccgtg acggtgattt
     6481 tcagggtgat caccagcccg tcgagcagat atggcaggga aggaacaatg gaactccagt
     6541 caaactcgta cattatttgc cccccatgtt gccgggcagg cgaactttac gttcaaccag
     6601 cgtcatcacc agcatgataa aagcgttaat caacacatac gtaagcgtga tggcggtaaa
     6661 cgactcccag gcgtgagccg agtagtcgag caatttaccc gcctgcgccg ccatatccac
     6721 cagaccgatg gtcgaggcga tggcggagtt tttcaccagg ttcatcatct cggaagtcat
     6781 cggcgggacg ataacgcgat aagcattagg cagcagtacg tatcgataag tctgcggtag
     6841 cgtcaggccc atcgccagcg cggcattctt ctgcccacgc ggtagcgact gaatagcggc
     6901 gcgtacctgt tcgcaaacac gggaggcggt aaacagcccc aggcagagca tggatgaaag
     6961 gaaaaactga atattgggat ccagctcggc cttaaaccac atgccgattt tctccggcag
     7021 cagctccggg atcaccagat accaggtaaa gaattgcaca atcaacggca cgttgcggaa
     7081 cagttcgaca tacaacgtac ccagaccgga gagaaaacgg tttggaacgg tacgtaaaat
     7141 gccgaaaaat gaaccgacga ggaaagcgat aatccaggcg cagatcgaca aagcgatcgt
     7201 cacctgaaaa ccactccaga cccaaccaag ataggtggta ttgccgaacg gggcttgttg
     7261 taaaaaaata ccccagttcc agtctataga cataaatcta ctccagaaaa aagagggtag
     7321 cagcgttaac tgctaccctc gaagattgtt acccagcgta ttgcggtttt caggcccgat
     7381 ggggaacgac catcaggcgt atagtctgtc cgtgctacgt aacaatcgag agggctggaa
     7441 tttccgcccc tggttcttgt aattagttca gtgccttgtc attcggttct ttgaacagtg
     7501 ctttcatttc gtctgacagt tcgaaattca tgttcaggtt tttcggcgga attggatttt
     7561 tgaaccattt atcaaaccat ttttccgctt caccggaggt ctgcacctga gcgatagtgt
     7621 catccatcag ctttttgaac tgcggatcgt ctttacgcaa catgcaaccg taggcttcct
     7681 gagactgcgg cttgccgacg atatcccagt tgtctggttt cttcgctttc gcacgttcac
     7741 cggccagcag agcgtcatcc atcataaagg caacggcacg accgctttcc agggtgcgga
     7801 aagagtcacc gtgatctttg gcgctgatga tgcgcatatt cattttttgc tcttcattca
     7861 gtttgttgag caaaacttca gaggtagtgc cggaagtgac gactacggct ttgcctttca
     7921 ggtcggcaaa atctttgata tcgccgccct ttttggtcaa caggcgcgta ccgaccacga
     7981 aaatagtgtc agagaaagcc gcctgttttt ggcgttcgac gttgttggtg gtagaaccac
     8041 attcaaaatc gaaagtgccg ttttgcagca gtggaatacg gtttgtgagg taatcggaat
     8101 cagttttacc tgcaagtccg gtttgttgag tttctttttc actgcttcaa caatggcgtt
     8161 ggagtaatcc tgcgagtaac ccaccacttt ttgctgattg tcgtaataag agaaaggtac
     8221 tgaagattca cggtgaccga cgacaatcac accgtttttg gcgattttgt ccagcgtact
     8281 gcctgctgcc ggggcggcgt catctgcctg tgccagtcct gcggaaagcg ccagggcgag
     8341 gattgctgtg gcaggtttac gtaattgcat atccaactcc tttatcttct gcgttaagaa
     8401 cgcatggata cccgttgtga gtgtttgtgt tgttatcgtc tgcaacttta ttgtgcagtg
     8461 ttgtgcctgt taggtaacat ttagtttggc taaatgtaaa gatattgatg ttttattgtt
     8521 tgtttttgcg aggtgcaccg caccattctg aagcaaaatt cttaaaatgc actcttttgg
     8581 tgctaccggt ggattgctgt ggtgcaaccg ggttgtactg atgctggttc aagctttgcc
     8641 ttgtataaca aagcaataga tgtgccaaag ttggatagga gaatattgtt atccggataa
     8701 tgcactgatg ccgcatccgg tgagcgtgcc gaaatatggg atgtattccg gcacgataag
     8761 aagggattat ttacgtcgct gacgcagact catcaacaca gcagcaaaac caaacagtgc
     8821 cgtcagcacc cacagcggcc agttgccggt acgtgcgtat ggtgtgagtc cggtggtcgg
     8881 cgtcacgtta gtggttaaca cctcgcgggt gaactgcggg atcatcgcct gaatctcacc
     8941 ctgcgggcca atcaccgccg taatgccgtt gttggtgctg cgcaacagtg ggcgcgccag
     9001 ctccagcgca cgcattcgcg ccatctggaa gtgttgccat ggaccaatag atttaccaaa
     9061 ccacgcatcg ttggagatag tcagcaggta gtcggtatcc gggcggaagt tatcgcgcac
     9121 ttgctcgccg agaatgatct cgtagcaaat agccgcagta agctcaatac catttgccga
     9181 cagcggcggc tggatatatg gcccacggct gaacgacgac atcggcagat caaagaacgg
     9241 tgctaacgga cgcagaatcg actccagcgg gacaaactcg ccaaacggca ccagatggtt
     9301 tttgttatag cgatcggctg attcgtagct gtacggtgca cctttaccca gcgtgatgat
     9361 ggtgttgtag gtatcgtagc ggttctgctt attgagacgc gcgtcgacaa tcccggttac
     9421 cagcgagcta cctttatcac gcaactcacc gtccagtgct ttgaggaacg gttgctgatt
     9481 aatttccaga tcggttatcg ccgactccgg ccagataatc aacgatgatt tgcccatcag
     9541 cggtgccgtt gcgttgtagt aaatctttag cgtattaaga agctggcctt cgtcccattt
     9601 cagcgattgc ggaatatcgc cctgaaccat cgaaacctga atggttttct ccggttgcgg
     9661 ggtaaaccat tggatgtaac gcagcgggaa gggtagggca aacagcacga cggccaccac
     9721 cagcggacgc cagttgcgtt tgaccaacgc cagtgccagc aggccactaa ccatcatcag
     9781 caggaagtta atggcttcca cgcccattag cggtgccagc ccttttaacg ggccatcaat
     9841 ctggctatag ccgaactgta accacgggaa gccagtcagc acccaaccgc gcagaaactc
     9901 ggtcacttgc cagagagcag gggcggcaat cgccacgcgc agccaggtgg ttttcggcca
     9961 cagacgcgac agcacgccag caaacagtcc ggtatacagc gacaaatacg ccgccagcag
    10021 caccaccagg aagatgttaa ccgggccagg catcccgcca aaggtcgcga tgctgacata
    10081 gacccagtta ataccgctgc caaagaggcc aaatccccag caaaagccaa tagcggcaga
    10141 ctggagtggg cgacggttaa aggtcaacgc ctgaagcccc atcagcgaaa taatcgccgc
    10201 aggccagacg tcgtaaggag agaaggccag cgttccgcag gcaccgaata ataacgccag
    10261 cagcaggcga atgcgctggc gttcaattaa tgaggcaaaa gccatgtagt tatctatcca
    10321 gtttcggttt tattcatcca gctttggctg gggtgagtca tccgggattt tgacatgaac
    10381 ctgaataata cgccgactat cggccatcgc cactttgaac tggtaaccgt cgatgtcgat
    10441 agtttcgcca cgcgccggaa gatgcccaaa cgcctgcatc accagaccac cgatagtgtc
    10501 gacttcttca tcgctaaagt gggtgccgaa cgcttcgttg aagtcttcaa tggaagccag
    10561 tgcgcgcacg gtccaggtat gacgactcag ctgacggaag tcgatatcat cttcttcgtc
    10621 atactcgtct tcaatctcac caacaatcag ttccaggatg tcttcaatgg tcaccagacc
    10681 ggaaacccca ccgaattcgt caataacgat cgccatgtgg taacgctgag agcgaaactc
    10741 tttcagcatc cggtctacgc gcttactttc aggaacgaca accgcctgac gtaacacttt
    10801 gtccatgccg aaggcttcag catcgctgcg cataaacggc agcaagtctt tcgccatcag
    10861 aatcccttca atgtgatctt tgtcttcgct aatcaccggg aaacgtgagt gggcggactc
    10921 gatgatgaca tcaaggcatt cgtccagcgt ctggttgcgt ttcagggtaa tcatctggga
    10981 gcgggggatc atgatgtcgc gaacgcgttg gtctgcgatg tccatcaccc cttcgagcat
    11041 atcgcgcgta tcttcgtcga taaggtcgtt ctgcccggaa tcacggatca gcgccagcag
    11101 ttcgtcacgg tttttcggtt caccgtggaa aagttggctg agtaacaggg agaaaaatcc
    11161 cttcttgttg cttatcgtgt cactactgtg tgaattgtcg tcgctcatgg cgtcgtatgg
    11221 gttctcatgt tagttaatca aaacgccgtc gttaatcacc aacggcgggg acgtctgcca
    11281 gtcaaatgcc tggcaaatta ttctttctcg gcaatgtacg gatcctcata gcccagagca
    11341 agcataatct ctgtttcgag ggcttccatt tcttctgctt cgtcatcttc aatgtgatcg
    11401 taacctaaca aatgcagact gccgtgcacc accatatgcg cccagtgcgc ctccagtggt
    11461 ttgccttgct cctgagcttc cttctcaacc acctgacggc agataaccag atcgcccagt
    11521 agcgacatct ccatgccagg cggcacttca aacgggaagg agagcacgtt ggtcggctta
    11581 tccttaccgc ggtaggtcag attcagactg tggctttcgg cggtatcgac catgcgaata
    11641 gtcacttccg actcttcctg aaactgcggg atcaccgcat tcagccatgt ttgaaactgg
    11701 ctctcttccg gtaacccgga attatcttca catgccagtt gtaaatcgag gatcacctga
    11761 ctcatttttg ttcctgttct tcgcgcttgc gttctgctgc cagcgccgct ttacgttttt
    11821 gttctgcttc ttccctggct tcataagcgt taacgatacg cgccaccacc gggtgacgaa
    11881 ccacgtcttc gctgtggaag aagttaaagc tgatctcttc gacatcggcc agcacttcaa
    11941 tggcatgacg taagcctgat ttagtattac gcggcaggtc gatctgtgtg acgtcgccgg
    12001 tgataaccgc ttttgagtta aaaccgatac gggtcaggaa catcttcatc tgttcgatgg
    12061 tagtgttctg gctctcatcg agaatgataa acgcgtcgtt cagcgtacga ccacgcatat
    12121 aggccagcgg cgcgacttca ataacgttgc gctcaatcag tttctcgact ttctcaaagc
    12181 ccagcatttc aaacagcgcg tcgtacagtg ggcgcagata cgggtctact ttctggctta
    12241 aatcgccagg caggaagccc agtttctcac cggcttcgac cgccggacga gtcagcagaa
    12301 tacggcgaat ttcctgacgc tccagggcat caactgccgc agccactgcc aggtaggttt
    12361 tacccgtacc cgccgggcca acgccgaagg taatgtcatg gtcgagaata ttggcgatgt
    12421 actgcgcctg gtttggcgtg cgcggcttaa ttacgccgcg tttagttttg atattgaccg
    12481 ctttgccgta ctccggcacg ctctccgcgc tttgctccag tacccgcgct tctttaatcg
    12541 caaggtggat ctgttccggt tcgatatcct gaatctgacc gcgcatcggg gcagtatcga
    12601 catacaggct acgcagaatg tctgccgcag cggtgacgca aatcggacgg ccggtcagtt
    12661 taaagtggtt atcgcggcga ttgatctcga tgccgagacg gcgttcgagc tgcttgatgt
    12721 tgtcatcaaa cgggccgcac aggctcaaca gacgcgcatt gtctgctggc tccagggtga
    12781 tttcgcgagt gtctatgttc aaaccgttcc tcttatctgt atgccgccgg aagctgaaca
    12841 ttcaccggcc tataaggaaa ttattcacgc cacaggaaaa aggcgcaagc attgcgataa
    12901 agatggggat aaagagagaa aaaacaaggc ccaccggaac ggcaggcctg agaattacgg
    12961 ctgataataa cccacgccaa ggtcgttttc tttgcgggtg cgggcaatca ccgactccgg
    13021 tgtttctgcc atgcgcagac ccatttcatc ttcagttcgc accactttac cgcgcagaga
    13081 gttcgggtag acgtcggtaa tttctacatc gacgaattta ccgatcatat ccggtgtgcc
    13141 ctcgaagttg accacgcggt tattttcggt acgcccggaa agctccatga tgctcttacg
    13201 cgaagtccct tctaccagaa tacgctgggt ggtgccgagc atccgacggc tccatgccat
    13261 cgcttgctga ttgatacgtt cttgcagaat atacagacgc tgcttcttct cttcttccgg
    13321 aacatcatca accatatcgg cggcaggcgt tcctggacgt gcagagaaga taaagctgta
    13381 gctcatgtcg aaattgacgt cggcaatcag cttcatcgtt ttctcgaagt cttcggtggt
    13441 ttcgccaggg aagccaacaa tgaagtcaga actgatctga atatccggac gcgccgcacg
    13501 cagtttacgg atgatcgctt tgtactccag cgccgtatgg gtacgtccca tcaggttcag
    13561 aatgcgatcg gaaccgctct gtaccggcag atgcaggaag ctcaccagct caggcgtgtc
    13621 gcggtacact tcgatgatat cgtcggtgaa ttcgatcgga tggctggtgg taaagcgaat
    13681 acgatcgatc ccgtcgatcg cagcaaccag acgcaacaga tcggcaaacg atccggtggt
    13741 gccgtcgtag ttctcaccac gccaggcatt cacattctga ccgagcaggt tgacttcacg
    13801 cacgccctga gccgcaagct gggcaatctc aaacagaata tcgtcagacg gacggcttac
    13861 ctcttcacca cgggtgtaag gcaccacgca gtaggtgcaa tatttattgc agccttccat
    13921 gatggagaca aacgcggtcg gcccttcggc gcgcggttcc ggcagacggt caaacttctc
    13981 gatttccggg aagctgatat ctacaaccgg gctgcggtcg ccgcgcacgg agttgatcat
    14041 ctccggcaga cggtgcagcg tttgcggccc aaaaataata tcgacatagt gggcgcgctg
    14101 gcgaatgtgc tcgccttctt gcgatgccac gcagccaccg acgccgataa tcaggtctgg
    14161 attcttctct tttaacagtt tccagcgacc caactgatgg aagacttttt cctgagcctt
    14221 ctcgcggatt gagcaggtgt tcagcagtag cacatccgct tcttccgcca cgtcggtcag
    14281 ttgatagccg tgggtggcat ccagcagatc ggccatcttc gatgaatcgt actcgttcat
    14341 ctgacagccc caggttttaa tatggagttt tttggtcatc gacttgctct tgcgaaatag
    14401 tagccaggaa tgcagggcgc atagtgtaat gctttgctgc cgttgtgacc agtatgagcg
    14461 ttatcagccc ttgggggtaa aaatcctgta aacttaaagc atattgctaa caggatgatt
    14521 gaccatgaca aatcaaccaa cggaaattgc cattgtcggc ggaggaatgg tcggcggcgc
    14581 actggcgctg gggctggcac agcacggatt ttcggtaacg gtgatcgagc acgcagaacc
    14641 tgcaccgttt gtcgctgaca gccaaccgga cgtgcggatc tcggcgatca gcgcggcttc
    14701 ggtatcattg cttaaagggg taggggtctg ggatgcagta caggctatgc gttgccatcc
    14761 ttaccgcaga ctggaaacct gggagtggga aacggcacat gtggtatttg acgcagccga
    14821 attaaagttg ccgttgcttg gctacatggt ggaaaacaca gtcctgcaac aggcgttgtg
    14881 gcaggcgctg gaagcgcatc cgaaagtaac gttacgtgtg ccaacctcgc tgattgcatt
    14941 acatcgagat aatgatcttc aggagctgga actgaagggc ggtgaaacga tccgcgcgaa
    15001 actggtgatt ggtgccgacg gcgcaaattc tcaggtgcgg cagatggcgg gaattggcgt
    15061 tcatgcctgg cagtatgcgc agtcgtgcat gttgattagc gtacagtgtg agaacgatcc
    15121 cggcgacagc acctggcagc aatttacccc ggacggaccg cgtgcgtttc tgccgttgtt
    15181 tgataactgg gcatcgctgg tgtggtatga ctctccggcg cgtattcgcc agttgcagaa
    15241 tatgaatatg gcgcaactcc aggcggaaat cgcgaagcat ttcccgtcgc gtctgggtta
    15301 cgttacaccg cttgccgctg gtgcgtttcc gctgacgcgc cgacatgcgt tgcagtatgt
    15361 gcagccgggg cttgcgctgg tgggcgatgc cgcgcatacc atccatccgc tggcggggca
    15421 gggggtgaat cttggttatc gtgatgtcga tgccctgatt gatgttctgg tgaatgcccg
    15481 cagctacggt gaagcgtggg ccagttatcc tgtcctcaag cgttaccaga tgcggcgcat
    15541 ggcggataac ttcattatgc aaagtggtat ggatctgttt tatgccggat tcagtaataa
    15601 tctgccgccg ctgcgttttg tgcgtaatct cgggttaatg gcggcagagc gtgctggcgt
    15661 gttgaaacgt caggcgctga aatatgcgtt agggttgtag ccttacaaca ttgccggatg
    15721 cgtgccaacc gtaggtcgga taagacgcgg cagcgtcgca tccgacattg aaggataaga
    15781 cgtgtcaaca tcgcattcga cattgaatga acgcagaaaa gcaaaaagct cgccgaagcg
    15841 agctttttta atgtggctgg ggtacgagga ttcgaacctc ggaatgccgg aatcagaatc
    15901 cggtgcctta ccgcttggcg ataccccaac aaattggttt ttgaatttgc cgaacgtatt
    15961 cgacacattc agaatttggt ggctacgacg ggattcgaac ctgtgacccc atcattatga
    16021 gtgatgtgct ctaaccaact gagctacgta gccagattat ttcttcgatg gctggggtac
    16081 ctggattcga accagggaat gccggtatca aaaaccggtg ccttaccgct tggcgatacc
    16141 ccaataaccg ggcggtgaac cgcttactcg aagaatatgg ctggggtacc tggattcgaa
    16201 ccagggaatg ccggtatcaa aaaccggtgc cttaccgctt ggcgataccc catccgtaca
    16261 acgctttctg gtgaatggtg cgggaggcga gacttgaact cgcacacctt gcggcgccag
    16321 aacctaaatc tggtgcgtct accaatttcg ccactcccgc aaaaaag
//
LOCUS       DADLXX010000093        16326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLXX010000093 DADLXX010000000
VERSION     DADLXX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.094"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="JNX27_002831"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="JNX27_002831"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7883973.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JNX27_002832"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JNX27_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7883974.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="JNX27_002833"
     CDS             2341..2556
                     /locus_tag="JNX27_002833"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7883975.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="JNX27_002834"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="JNX27_002834"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7883976.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /locus_tag="JNX27_002835"
     CDS             4451..5845
                     /locus_tag="JNX27_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7883977.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="JNX27_002836"
     CDS             5922..6419
                     /locus_tag="JNX27_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7883978.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="JNX27_002837"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="JNX27_002837"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7883979.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="JNX27_002838"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="JNX27_002838"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7883980.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="JNX27_002839"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="JNX27_002839"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7883981.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9032..9667
                     /locus_tag="JNX27_002840"
     CDS             9032..9667
                     /locus_tag="JNX27_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7883982.1"
                     /translation="MTAPFFPRILAAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="JNX27_002841"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="JNX27_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7883983.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="JNX27_002842"
     CDS             complement(10259..11239)
                     /locus_tag="JNX27_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7883984.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="JNX27_002843"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="JNX27_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7883985.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="JNX27_002844"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="JNX27_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7883986.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="JNX27_002845"
     CDS             12542..12832
                     /locus_tag="JNX27_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7883987.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="JNX27_002846"
     CDS             12840..13433
                     /locus_tag="JNX27_002846"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7883988.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAEMTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="JNX27_002847"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="JNX27_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7883989.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGMQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="JNX27_002848"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="JNX27_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..>16326
                     /locus_tag="JNX27_002849"
     CDS             15884..>16326
                     /locus_tag="JNX27_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY7883990.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4042 a         4205 c         4110 g         3969 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctgcgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agagcatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc tacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacactacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa aaaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg tcgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cagcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ttgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcacttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggtgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatagccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 ggccgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggcgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg atgcagtggg
    12061 ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aaatgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 tcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtatg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaagcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tgacgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa ggcaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 tacccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccgc gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgc
//
LOCUS       DADLXX010000094        16098 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLXX010000094 DADLXX010000000
VERSION     DADLXX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.095"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="JNX27_002850"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="JNX27_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7883991.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGILLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="JNX27_002851"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="JNX27_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7883992.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="JNX27_002852"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="JNX27_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7883993.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="JNX27_002853"
     CDS             complement(1511..1978)
                     /locus_tag="JNX27_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7883994.1"
                     /translation="MDTTLPFLILYACLSDLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="JNX27_002854"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="JNX27_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7883995.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="JNX27_002855"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="JNX27_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7883996.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="JNX27_002856"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="JNX27_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7883997.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="JNX27_002857"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="JNX27_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7883998.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="JNX27_002858"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="JNX27_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7883999.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="JNX27_002859"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="JNX27_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7884000.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="JNX27_002860"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="JNX27_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7884001.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="JNX27_002861"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="JNX27_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7884002.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="JNX27_002862"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="JNX27_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7884003.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="JNX27_002863"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="JNX27_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7884004.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="JNX27_002864"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="JNX27_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7884005.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="JNX27_002865"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="JNX27_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7884006.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="JNX27_002866"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="JNX27_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7884007.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="JNX27_002867"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="JNX27_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7884008.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="JNX27_002868"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="JNX27_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7884009.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="JNX27_002869"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="JNX27_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7884010.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9488..10021
                     /gene="rplF"
                     /locus_tag="JNX27_002870"
     CDS             9488..10021
                     /gene="rplF"
                     /locus_tag="JNX27_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7884011.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10031..10384
                     /gene="rplR"
                     /locus_tag="JNX27_002871"
     CDS             10031..10384
                     /gene="rplR"
                     /locus_tag="JNX27_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7884012.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10399..10902
                     /gene="rpsE"
                     /locus_tag="JNX27_002872"
     CDS             10399..10902
                     /gene="rpsE"
                     /locus_tag="JNX27_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7884013.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10906..11085
                     /gene="rpmD"
                     /locus_tag="JNX27_002873"
     CDS             10906..11085
                     /gene="rpmD"
                     /locus_tag="JNX27_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7884014.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11089..11523
                     /gene="rplO"
                     /locus_tag="JNX27_002874"
     CDS             11089..11523
                     /gene="rplO"
                     /locus_tag="JNX27_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7884015.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11531..12862
                     /gene="secY"
                     /locus_tag="JNX27_002875"
     CDS             11531..12862
                     /gene="secY"
                     /locus_tag="JNX27_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7884016.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12894..13010
                     /gene="rpmJ"
                     /locus_tag="JNX27_002876"
     CDS             12894..13010
                     /gene="rpmJ"
                     /locus_tag="JNX27_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7884017.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13157..13513
                     /gene="rpsM"
                     /locus_tag="JNX27_002877"
     CDS             13157..13513
                     /gene="rpsM"
                     /locus_tag="JNX27_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7884018.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13530..13919
                     /gene="rpsK"
                     /locus_tag="JNX27_002878"
     CDS             13530..13919
                     /gene="rpsK"
                     /locus_tag="JNX27_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7884019.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13953..14573
                     /gene="rpsD"
                     /locus_tag="JNX27_002879"
     CDS             13953..14573
                     /gene="rpsD"
                     /locus_tag="JNX27_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7884020.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14599..15588
                     /gene="rpoA"
                     /locus_tag="JNX27_002880"
     CDS             14599..15588
                     /gene="rpoA"
                     /locus_tag="JNX27_002880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7884021.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15629..16012
                     /gene="rplQ"
                     /locus_tag="JNX27_002881"
     CDS             15629..16012
                     /gene="rplQ"
                     /locus_tag="JNX27_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7884022.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4140 a         3788 c         4291 g         3879 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tattctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc cgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ccaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag gtctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcacaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac tgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgtat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcctgg gtattgtaaa accatggaac tctacctggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaag aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggtaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattgttgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaaatcgga
     9481 ggaaaaaatg tctcgtgttg ctaaagcacc ggtcgttgtt cctgccggcg ttgacgtaaa
     9541 aatcaacggt caggttatta cgatcaaagg taaaaacggc gagctgactc gtactctcaa
     9601 cgatgctgtt gaagttaaac atgcagataa taccctgacc ttcggtccgc gtgatggtta
     9661 cgcagacggt tgggcacagg ctggtaccgc gcgtgccctg ctgaactcaa tggttatcgg
     9721 tgttaccgaa ggcttcacta agaagctgca gctggttggt gtaggttacc gtgcagcggt
     9781 taaaggcaat gtgattaacc tgtctctggg tttctctcat cctgttgacc atcagctgcc
     9841 tgcgggtatc actgctgaat gtccgactca gactgaaatc gtgctgaaag gcgctgataa
     9901 gcaggtgatc ggccaggttg cagcggatct gcgcgcctac cgtcgtcctg agccttacaa
     9961 aggcaagggt gttcgttacg ccgacgaagt cgtgcgtacc aaagaggcta agaagaagta
    10021 aggtaacact atggataaga aatctgctcg tatccgtcgt gcgacccgcg cacgccgcaa
    10081 gctccaggag ctgggcgcaa ctcgcctggt ggtacatcgt accccgcgtc acatttacgc
    10141 acaggtaatt gcaccgaacg gttctgaagt tctggtagct gcttctactg tagaaaaagc
    10201 tatcgctgaa caactgaagt acaccggtaa caaagacgcg gctgcagctg tgggtaaagc
    10261 tgtcgctgaa cgcgctctgg aaaaaggcat caaagatgta tcctttgacc gttccgggtt
    10321 ccaatatcat ggtcgtgtcc aggcactggc agatgctgcc cgtgaagctg gccttcagtt
    10381 ctaaggtaga ggtgtaagat ggctcacatc gaaaaacaag ctggcgaact gcaggaaaag
    10441 ctgatcgcgg taaaccgcgt atctaaaacc gttaaaggtg gtcgtatttt ctccttcaca
    10501 gctctgactg tagttggcga tggtaacggt cgcgttggtt ttggttacgg taaagcgcgt
    10561 gaagttccag cagcgatcca gaaagcgatg gaaaaagccc gtcgcaatat gattaacgtc
    10621 gcgctgaata acggcactct acaacaccct gttaaaggtg ttcacacggg ttctcgcgta
    10681 ttcatgcagc cggcttccga aggtaccggt atcatcgccg gtggtgcaat gcgcgccgtt
    10741 ctggaagtcg ctggggttca taacgttctg gctaaagcat atggttccac caacccgatc
    10801 aacgtggttc gtgcaactat tgatggccta gaaaatatga attctccaga aatggtcgct
    10861 gccaagcgtg gtaaatccgt tgaagaaatt ctggggaaat aaaccatggc aaagactatt
    10921 aaaattactc aaacccgcag tgcaatcggt cgtctgccga aacacaaggc aacgctgctt
    10981 ggcttgggtc tgcgtcgtat tggtcacacc gtagagcgcg aggatactcc tgctattcgc
    11041 ggtatgatca acgcggtttc cttcatggtt aaagttgagg agtaagagat gcgtttaaat
    11101 actctgtctc cggccgaagg ctccaaaaag gcgggtaaac gcctggggcg tggtatcggt
    11161 tctggcctcg gtaaaaccgg tggtcgtggt cacaaaggtc agaagtctcg ttctggcggt
    11221 ggcgtacgtc gcggtttcga gggtggtcag atgcctctgt accgtcgtct gccgaaattc
    11281 ggcttcactt ctcgtaaagc agcgattaca gccgaagttc gtctatctga cctggctaaa
    11341 gtagaaggcg gtgttgtaga cctgaacacg ctgaaagcgg ctaacattat cggtatccag
    11401 atcgagttcg cgaaagtgat cctggctggc gaagtaacga ctccggtaac tgttcgtggc
    11461 ctgcgtgtta ctaaaggcgc tcgtgctgct atcgaagctg ctggcggtaa aatcgaggaa
    11521 taagtagcag atggctaaac aaccgggatt agattttcaa agtgccaaag gtggcttagg
    11581 cgagctgaaa cgcagactgc tgtttgttat cggtgcgctg attgtgttcc gtattggctc
    11641 ttttattccg atccctggta ttgatgccgc tgtacttgcc aaactgcttg agcaacagcg
    11701 aggcaccatc attgagatgt ttaacatgtt ctctggtggt gctctcagcc gtgcttctat
    11761 ctttgctctg gggatcatgc cgtatatttc ggcgtcgatc attatccagc tgctgacggt
    11821 ggttcaccca acgttggcag aaattaagaa agaaggggag tctggtcgtc gtaagatcag
    11881 ccagtacacc cgttacggta ctctggtgct ggcaatattc cagtcgatcg gtattgctac
    11941 cggtctgccg aatatgcctg gtatgcaagg cctggtgatt aacccgggct ttgcattcta
    12001 cttcaccgct gttgtaagtc tggtcacagg aacgatgttc ctgatgtggt tgggcgaaca
    12061 gatcactgaa cgaggtatcg gcaacggtat ttcaatcatt atcttcgccg gtattgtcgc
    12121 gggactcccg ccagccattg cccatactat cgagcaagcg cgtcaaggcg acctgcactt
    12181 cctcgtgttg ctgttggttg cagtattagt atttgcagtg acgttctttg ttgtgtttgt
    12241 tgagcgtggt caacgccgca ttgtggtaaa ctacgcgaaa cgtcagcaag gtcgtcgtgt
    12301 ctatgctgca cagagcacac atttaccgct gaaagtgaat atggcggggg taatcccggc
    12361 aatcttcgct tccagtatta ttctgttccc ggcgaccatc gcgtcatggt tcgggggcgg
    12421 tactggttgg aactggctga caacaatttc gctgtatttg cagcctgggc aaccgcttta
    12481 tgtgttactc tatgcgtctg caatcatctt cttctgtttc ttctacacgg cgttggtttt
    12541 caacccgcgt gaaacagcag ataacctgaa gaagtccggt gcatttgtac caggaattcg
    12601 tccgggagag caaacggcga agtatatcga taaagtaatg acccgcctga ccttggttgg
    12661 tgcgctgtat attaccttta tctgcctgat cccggagttc atgcgtgatg caatgaaagt
    12721 accgttctac ttcggtggga cctcactgct tatcgttgtt gtcgtgatta tggactttat
    12781 ggctcaagtg caaactctga tgatgtccag tcagtatgag tctgcattga agaaggcgaa
    12841 cctgaaaggc tacggccgat aattggtcgc ccgagaagtt acggagagta aaaatgaaag
    12901 ttcgtgcttc cgtcaagaaa ttatgccgta actgcaaaat cgttaagcgt gatggtgtca
    12961 tccgtgtgat ttgcagtgcc gagccgaagc ataaacagcg ccaaggctga ttttttcgca
    13021 tatttttctt gcaaagttgg gttgagctgg ctagattagc cagccaatct tttgtatgtc
    13081 tgtgcgtttc catttgagta tcctgaaaac gggcttttca gcatggaacg tacatattaa
    13141 atagtaggag tgcatagtgg cccgtatagc aggcattaac attcctgatc ataagcatgc
    13201 cgtaatcgca ttaacttcga tttatggcgt cggcaagacc cgttctaaag ccatcctggc
    13261 tgcagcgggt atcgctgaag atgttaagat cagtgagctg tctgaaggac aaatcgacac
    13321 gctgcgtgac gaagttgcca aatttgtcgt tgaaggtgat ctgcgccgtg aaatcagcat
    13381 gagcatcaag cgcctgatgg atcttggttg ctatcgcggt ttgcgtcatc gtcgtggtct
    13441 cccggttcgc ggtcagcgta ccaagaccaa cgcacgtacc cgtaagggtc cgcgcaaacc
    13501 gatcaagaaa taatcggggt gattgaataa tggcaaaggc accaattcgt gcacgtaaac
    13561 gtgtaagaaa acaagtctct gacggcgtgg ctcatatcca tgcttctttc aacaacacca
    13621 tcgtgactat cactgatcgt cagggtaacg cgttgggttg ggcaacagcc ggtggttccg
    13681 gtttccgtgg ttctcgcaaa tccactccgt ttgcagctca ggttgcagca gagcgttgcg
    13741 ctgacgccgt gaaagaatac ggcatcaaga atctggaagt tatggttaaa ggtccgggtc
    13801 caggccgcga atctactatt cgtgctctga acgccgcagg tttccgcatc actaacatta
    13861 ctgatgtgac tccgatccct cataacggtt gtcgtccgcc gaaaaaacgt cgcgtataac
    13921 gcctcgtttt ccaggtttgt tggagaaaga aaatggcaag atatttgggt cctaagctca
    13981 agctgagccg tcgtgagggc accgacttat tccttaagtc tggcgttcgc gcgatcgata
    14041 ccaagtgtaa aattgaacaa gctcctggcc agcacggtgc gcgtaaaccg cgtctgtctg
    14101 actatggtgt gcagttgcgt gaaaagcaaa aagttcgccg tatctatggt gtgctggagc
    14161 gtcagttccg taactactac aaagaagcag cacgtctgaa aggcaacacc ggtgaaaacc
    14221 tgttggctct gctggaaggt cgtctggaca acgttgtata ccgtatgggc ttcggtgcca
    14281 ctcgtgcaga agcacgtcag ctggttagcc ataaagcaat tatggtaaac ggtcgtgttg
    14341 ttaacatcgc ttcttatcag gttagtccga atgacgttgt aagcattcgt gagaaagcga
    14401 agaagcagtc tcgcgtgaaa gccgctctgg agctggctga gcagcgtgaa aagccaactt
    14461 ggctggaagt tgatgctggt aagatggaag gtacgtttaa gcgtaagccg gagcgttctg
    14521 atctgtctgc ggacattaac gaacacctga tcgtcgagct ttactccaag taaagcttag
    14581 taccaaagag aggacacaat gcagggttct gtgacagagt ttctaaaacc gcgcctggtt
    14641 gatatcgagc aagtgagttc gacgcacgcc aaggtgaccc ttgagccttt agagcgtggc
    14701 tttggccata ctctgggtaa cgcactgcgc cgtattctgc tctcatcgat gccgggttgc
    14761 gcggtgaccg aggttgagat tgatggtgta ctacatgagt acagcaccaa agaaggcgtt
    14821 caggaagata tcctggaaat cctgctcaac ctgaaagggc tggcggtgag agttcagggc
    14881 aaagatgaag ttattcttac cttgaataaa tctggcattg gccctgtgac tgcagccgat
    14941 atcacccacg acggtgatgt cgaaatcgtc aagccgcagc acgtgatctg ccacctgacc
    15001 gatgagaacg cgtctattag catgcgtatc aaagttcagc gcggtcgtgg ttatgtgccg
    15061 gcttctaccc gaattcattc ggaagaagat gagcgcccaa tcggccgtct gctggtcgac
    15121 gcatgctaca gccctgtgga gcgtattgcc tacaatgttg aagcagcgcg tgtagaacag
    15181 cgtaccgacc tggacaagct ggtcatcgaa atggaaacca acggcacaat cgatcctgaa
    15241 gaggcgattc gtcgtgcggc aaccattctg gctgaacaac tggaagcttt cgttgactta
    15301 cgtgatgtac gtcagcctga agtgaaagaa gagaaaccag agttcgatcc gatcctgctg
    15361 cgccctgttg acgatctgga attgactgtc cgctctgcta actgccttaa agcagaagct
    15421 atccactata tcggtgatct ggtacagcgt accgaggttg agctccttaa aacgcctaac
    15481 cttggtaaaa aatctcttac tgagattaaa gacgtgctgg cttcccgtgg actgtctctg
    15541 ggcatgcgct tggaaaactg gccaccggca agcatcgctg acgagtaacc ggatcacagg
    15601 ttaaggtttt actgagaagg ataaggtcat gcgccatcgt aagagtggtc gtcaactgaa
    15661 ccgcaacagc agccatcgcc aggctatgtt ccgcaatatg gcaggttcac tggttcgtca
    15721 tgaaatcatc aagacgactc tgcctaaagc gaaagagctg cgccgcgtag ttgagccgct
    15781 gattactctt gccaagactg atagcgttgc taatcgtcgt ctggcattcg cccgtactcg
    15841 tgataacgag atcgtggcaa aactgtttaa cgaactgggc ccgcgtttcg cgagccgtgc
    15901 cggtggttac actcgtattc tgaagtgtgg cttccgtgca ggcgacaacg cgccgatggc
    15961 ttacatcgag ctggttgatc gttcagagaa agcagaagct gctgcagagt aatctgaagc
    16021 aacgtaaaaa aacccgcccc ggcgggtttt tttatacccg tagtatcccc acttatctac
    16081 aatagctgta ctcttttt
//
LOCUS       DADLXX010000095        16030 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLXX010000095 DADLXX010000000
VERSION     DADLXX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.096"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNX27_002882"
     CDS             complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNX27_002882"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7884023.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JNX27_002883"
     CDS             1020..1703
                     /locus_tag="JNX27_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY7884024.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JNX27_002884"
     CDS             1700..2161
                     /locus_tag="JNX27_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884025.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JNX27_002885"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JNX27_002885"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7884026.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JNX27_002886"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JNX27_002886"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7884027.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSRAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNX27_002887"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNX27_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7884028.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JNX27_002888"
     CDS             complement(4704..4787)
                     /locus_tag="JNX27_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7884029.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="JNX27_002889"
     CDS             5380..6210
                     /locus_tag="JNX27_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7884030.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYFGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNX27_002890"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNX27_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7884031.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="JNX27_002891"
     CDS             complement(7339..8799)
                     /locus_tag="JNX27_002891"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7884032.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGSAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNX27_002892"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNX27_002892"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7884033.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="JNX27_002893"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="JNX27_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY7884034.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNX27_002894"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNX27_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7884035.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNX27_002895"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNX27_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7884036.1"
                     /translation="MKWCKRGYVLAAMLALASATIQPADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNX27_002896"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNX27_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY7884037.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNX27_002897"
     CDS             complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNX27_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY7884038.1"
                     /translation="SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSG
                     SKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRG
                     FAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGS
                     TQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYG
                     GTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNES
                     GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGK
                     LQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAW
                     QKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED
                     NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL
                     AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL
                     DTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSE
                     SSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAE
                     CR"
BASE COUNT         3861 a         4136 c         3923 g         4110 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccgg gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattattt tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcactac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcggcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctggctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact
//
LOCUS       DADLXX010000096        15993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLXX010000096 DADLXX010000000
VERSION     DADLXX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.097"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            9..746
                     /locus_tag="JNX27_002898"
     CDS             9..746
                     /locus_tag="JNX27_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7884039.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JNX27_002899"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JNX27_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7884040.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="JNX27_002900"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="JNX27_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7884041.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JNX27_002901"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JNX27_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7884042.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..6724
                     /locus_tag="JNX27_002902"
                     /pseudo
     CDS             5041..6724
                     /locus_tag="JNX27_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            6776..7931
                     /locus_tag="JNX27_002903"
                     /pseudo
     CDS             6776..7931
                     /locus_tag="JNX27_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(7897..8019)
                     /locus_tag="JNX27_002904"
     CDS             complement(7897..8019)
                     /locus_tag="JNX27_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884043.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8222..9283
                     /locus_tag="JNX27_002905"
     CDS             8222..9283
                     /locus_tag="JNX27_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY7884044.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLINKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTE"
     gene            9377..9907
                     /locus_tag="JNX27_002906"
                     /pseudo
     CDS             9377..9907
                     /locus_tag="JNX27_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            9945..12317
                     /locus_tag="JNX27_002907"
     CDS             9945..12317
                     /locus_tag="JNX27_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7884045.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12362..15157
                     /locus_tag="JNX27_002908"
     CDS             12362..15157
                     /locus_tag="JNX27_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7884046.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETF
                     ESMGLRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVISEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            15519..>15993
                     /locus_tag="JNX27_002909"
     CDS             15519..>15993
                     /locus_tag="JNX27_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7884047.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLV"
BASE COUNT         4676 a         3493 c         3504 g         4320 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
     5341 gccattgaag ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
     5401 cgttttacta acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg
     5461 accggtcgag ctcccgcccg cgttggtgtc tattccaata ccgatgctca ggatggtatt
     5521 ccgctaacag aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca
     5581 gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg
     5641 cgtgactatc atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt
     5701 ggctttgatt actttatggg attccacgct gcaggaacgg catattacaa ctccccttca
     5761 ctgttcaaaa atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat
     5821 gaggcaattg gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg
     5881 gcttataatg ctccgcacct accaaatgat aatcctgcac cggatcaata tcagaagcaa
     5941 tttaataccg gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag
     6001 ggtgtaaaac gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt
     6061 ctctttacct ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa
     6121 aaaggctata agagtcagac ctatcctggc ggtactcacc accccaatgt ttatgtggtg
     6181 gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc
     6241 aacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg atggtgtttc
     6301 cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat
     6361 aacctcttat tctcactggt ttgatgagga aaatattcca ttctgggata attaccacaa
     6421 atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca
     6481 attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag aaaacaatca
     6541 gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc
     6601 gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc
     6661 gcttagcgag gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa
     6721 ataactaaac cttcatgcgg cggatttttc cgccgcctta ttgagcgaga tagcaatgca
     6781 cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct
     6841 tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc tgaaagagtt
     6901 catcaaacaa tatatcgaag cgtctggcaa tcaggtctat tttacctggc aaggcggtga
     6961 acccactctg gctggcctgg attttttccg taaagttatt cactatcaac aacgctatgc
     7021 aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg
     7081 gtgtgccttt ctcaaagaac atgattttct ggttggtatc tcgatcgatg gcccccagga
     7141 gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc
     7201 agccatcgag cgtctgaaat catatcaagt agagtttaat acgttaaccg tcattaataa
     7261 cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca
     7321 tatgcaattt atcgaattgc tagaaaccgg gacgccgaat attgatttca gtggtcatag
     7381 tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat
     7441 gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt
     7501 tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt tccaggagtc
     7561 ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt
     7621 ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt
     7681 acaactgaca gcgcaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     7741 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     7801 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     7861 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     7921 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     7981 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     8041 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     8101 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     8161 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     8221 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     8281 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     8341 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     8401 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     8461 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     8521 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     8581 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     8641 cgctgaagac attctcttac tgatcaacaa aacgctcagt ctttcatttg gcttcatcca
     8701 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     8761 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     8821 cattgtgatc ggtggaactt tatttaccca taaggtgggt aaacgtattc gtccgcttaa
     8881 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     8941 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     9001 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     9061 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     9121 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     9181 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     9241 ggttatcgat cgcttgtatg agttccatca actcactgaa tagcgcccta cgaataagcc
     9301 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     9361 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     9421 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtgatt
     9481 taaaggtgat atttcttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     9541 aagcggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     9601 gttgagcgaa gtactgcatc aggttggtct ggggaaattg gctgcgcgta ttcatgacca
     9661 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     9721 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     9781 agaggctata cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     9841 ggttacacat caacccggtg tctggaacct ggctgatgat atttgtgaca ttagcacggt
     9901 tttataacaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     9961 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
    10021 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
    10081 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
    10141 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
    10201 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
    10261 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
    10321 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
    10381 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
    10441 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
    10501 gcactacgcg tttggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
    10561 atcaaggttc ttcaggaagt acctatttct ctccagattt taaaaagaac ttttatacct
    10621 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
    10681 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac
    10741 agtataaaaa cgttatcgat actgcattga gcaaatttac ctggtttgcc agcgaccgct
    10801 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagcacct
    10861 tcccgcaatc tgatcgcgaa atgggtaata aatcctatgg tctggcatgg gatatggata
    10921 cgcaactcgc atgggccaaa ctacgtacca ccgttggttg ggatcatatt agtgattata
    10981 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
    11041 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
    11101 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctacttcg
    11161 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
    11221 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
    11281 tgggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
    11341 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
    11401 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
    11461 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
    11521 cggaatcggt atcaggtaat aaaaccctga cgcgttatca gaatttgaaa acgccttata
    11581 acgatgaact ggcaatggga ttgcagcaaa aaatcgataa gaacgttatt gcacgcgcaa
    11641 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
    11701 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
    11761 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
    11821 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
    11881 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
    11941 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
    12001 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
    12061 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
    12121 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
    12181 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
    12241 ttgatacaac gaacaccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
    12301 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
    12361 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
    12421 ccccgggcgg ctgattgccg ccgccttaca gcaggatgaa aagttaatta ccgggcaact
    12481 ggacaatggc ttgcgatata tgatttatcc gcatgctcat ccaaaggatc aggtaaattt
    12541 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
    12601 ttttgtagaa catatgatgt ttaacggcgc aaaaacatgg ccgggtaata aagtcatcga
    12661 aacatttgag tcaatgggcc tgcgttttgg tctcgatgtt aatgcctata ccagctatga
    12721 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
    12781 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
    12841 acgtggcgta atttctgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
    12901 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
    12961 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
    13021 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc
    13081 gctgataaag gataatttaa gtaagtttcc ggctaacaaa gcagctgaaa atcgcgtctg
    13141 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
    13201 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
    13261 tgtcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
    13321 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
    13381 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc
    13441 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
    13501 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
    13561 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
    13621 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
    13681 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
    13741 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
    13801 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
    13861 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
    13921 tctgacatca ttaacacttt ccaatggtgc caaggttatt ctggcaaaat ccgcgggtga
    13981 agagcaaaag ctacaaatta ttgccgtatc gaataaaggc gatttaagtt tccctgcgca
    14041 gcaaaaatca cttatcgcgc tggcaaataa agcagttagc ggaagcggcg ttggcgaact
    14101 ctcctcttcc agcctgaaac gctggagtgc ggaaaactcg gtaaccatga gcagtaaagt
    14161 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
    14221 ccagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
    14281 acaaaatgct caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc
    14341 ccagctgatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
    14401 gattgcacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
    14461 agcggatatc acgtttgtca ttgtcggtaa tgtcgcagaa gacaaactcg tggcgttaat
    14521 tacgcgttac ttaggctcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
    14581 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
    14641 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
    14701 gctcgatgct tttaacgtcg cactggcaaa agatctacgc gttaatattc gtgaacaggc
    14761 atctggggca tacagcgttt cttctcgcct ctcggttgat cctcaggcta aagatatcag
    14821 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaacagttaa cgttagcgaa
    14881 tgaagtgatg gttaagcgtt tgactaaggg gatcagtgag caagaactga acgaatacca
    14941 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
    15001 tattataaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt
    15061 gttgaaacaa atgacgatag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
    15121 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
    15181 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
    15241 ataataacca agtactaata gtgatatttt aatgtctgat ttttacgtga taattcagga
    15301 ggcacagaaa gcgcataaaa ataacaacat aaaacacctt gccaccaccc aagaattctc
    15361 tattgcattg tttttaatga aaaaaatatc attccaacaa tatctcacga taaataacat
    15421 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
    15481 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
    15541 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
    15601 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
    15661 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
    15721 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
    15781 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt
    15841 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
    15901 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
    15961 caggcaaacc aacggataaa ccaaacctgg tgt
//
LOCUS       DADLXX010000097        15952 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLXX010000097 DADLXX010000000
VERSION     DADLXX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.098"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNX27_002910"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNX27_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY7884048.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JNX27_002911"
     CDS             complement(998..2020)
                     /locus_tag="JNX27_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7884049.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JNX27_002912"
     CDS             complement(2034..3536)
                     /locus_tag="JNX27_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7884050.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNX27_002913"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNX27_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JNX27_002914"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JNX27_002914"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7884051.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JNX27_002915"
     CDS             complement(5853..6194)
                     /locus_tag="JNX27_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7884052.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNX27_002916"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNX27_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7884053.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNX27_002917"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNX27_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7884054.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="JNX27_002918"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="JNX27_002918"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7884055.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="JNX27_002919"
     CDS             12873..14216
                     /locus_tag="JNX27_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7884056.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="JNX27_002920"
     CDS             complement(14278..14484)
                     /locus_tag="JNX27_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7884057.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKMHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15363
                     /locus_tag="JNX27_002921"
     CDS             14809..15363
                     /locus_tag="JNX27_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7884058.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15426..>15952)
                     /locus_tag="JNX27_002922"
     CDS             complement(15426..>15952)
                     /locus_tag="JNX27_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884059.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3796 a         4371 c         3921 g         3864 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agcagacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtcctt tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgtg ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agctaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaatccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 catcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 ccctggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gaagaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac
    15301 ggggcgctca caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa
    15361 taatggcaat aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag
    15421 gggaattaag tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt
    15481 ttaaacgcct caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta
    15541 tctgcggcca gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg
    15601 caacctgcat taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca
    15661 gaggaaccat tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat
    15721 tctatgacta tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcgcat
    15781 tctttaattc caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact
    15841 acatcctcca ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct
    15901 ttaatccttt ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc at
//
LOCUS       DADLXX010000098        15888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLXX010000098 DADLXX010000000
VERSION     DADLXX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.099"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..615)
                     /locus_tag="JNX27_002923"
     CDS             complement(<1..615)
                     /locus_tag="JNX27_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7884060.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
     gene            complement(612..1874)
                     /locus_tag="JNX27_002924"
                     /pseudo
     CDS             complement(612..1874)
                     /locus_tag="JNX27_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1871..2779)
                     /locus_tag="JNX27_002925"
     CDS             complement(1871..2779)
                     /locus_tag="JNX27_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7884061.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2945..3742
                     /locus_tag="JNX27_002926"
     CDS             2945..3742
                     /locus_tag="JNX27_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7884062.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3793..4445)
                     /gene="pphB"
                     /locus_tag="JNX27_002927"
                     /pseudo
     CDS             complement(3793..4445)
                     /gene="pphB"
                     /locus_tag="JNX27_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4551..7112)
                     /gene="mutS"
                     /locus_tag="JNX27_002928"
     CDS             complement(4551..7112)
                     /gene="mutS"
                     /locus_tag="JNX27_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7884063.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7188..7367
                     /locus_tag="JNX27_002929"
     CDS             7188..7367
                     /locus_tag="JNX27_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884064.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMFMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7399..7752
                     /locus_tag="JNX27_002930"
     CDS             7399..7752
                     /locus_tag="JNX27_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7884065.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7789..9867)
                     /gene="flhA"
                     /locus_tag="JNX27_002931"
     CDS             complement(7789..9867)
                     /gene="flhA"
                     /locus_tag="JNX27_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY7884066.1"
                     /translation="MSYTPMSGLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9941..10951)
                     /gene="hypE"
                     /locus_tag="JNX27_002932"
     CDS             complement(9941..10951)
                     /gene="hypE"
                     /locus_tag="JNX27_002932"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7884067.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10948..12069)
                     /gene="hypD"
                     /locus_tag="JNX27_002933"
     CDS             complement(10948..12069)
                     /gene="hypD"
                     /locus_tag="JNX27_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7884068.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12069..12341)
                     /gene="hypC"
                     /locus_tag="JNX27_002934"
     CDS             complement(12069..12341)
                     /gene="hypC"
                     /locus_tag="JNX27_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7884069.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVSFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12332..13204)
                     /gene="hypB"
                     /locus_tag="JNX27_002935"
     CDS             complement(12332..13204)
                     /gene="hypB"
                     /locus_tag="JNX27_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7884070.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13208..13558)
                     /gene="hypA"
                     /locus_tag="JNX27_002936"
     CDS             complement(13208..13558)
                     /gene="hypA"
                     /locus_tag="JNX27_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7884071.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13770..14231
                     /gene="hycA"
                     /locus_tag="JNX27_002937"
     CDS             13770..14231
                     /gene="hycA"
                     /locus_tag="JNX27_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7884072.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14356..14967
                     /locus_tag="JNX27_002938"
     CDS             14356..14967
                     /locus_tag="JNX27_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7884073.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPLLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14964..>15888
                     /locus_tag="JNX27_002939"
     CDS             14964..>15888
                     /locus_tag="JNX27_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY7884074.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGAMSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGIILL"
BASE COUNT         3624 a         4256 c         4206 g         3802 t
ORIGIN      
        1 acctgcggtc agataacgga tcgggcggtc accgagaata aaaatcagct ttgccgtttc
       61 ctccagctct tccatattgt tggcggcttc ttgcaggctt tcaccgcaaa ccactgggcc
      121 atgatttgcc agtaaaaaag cctgattgtc tgctgccagt tccgccagat cctgtgcgat
      181 gcgtttatcg cccggtcggt aatagggcac cagcgggaca tttcccatcc gcatcaccac
      241 gtatggtgtg aacggacgaa taacgttgct gctgtccagc ccttgcaggc aggaaagcgc
      301 cgtcgaccat gtgctgtgca aatgcaccac cgctttacag cgcggattgt tgcgatacag
      361 cgccagatga aagagcacct ctttcgaggg tttgtcacca cttaaccatt cgccatccgc
      421 ggcgactttg gaaagccgct gcggatcgag attgcccagg catgaacctg tcggtgtcgc
      481 cagtaaattc ccgtcaggta aaagcagcga cagattgcca gccgaaccgg ttgcatagcc
      541 gcgctgaaag aatgaactgg caatccgcgt catctcctct cgcaaagact gctctacttt
      601 tgcgaaatcg ctcatgataa aaactctctt tgggctcgtg aaaaaaaggc ttcatcaccg
      661 aagttgccag atttaagggc gagtgagaca ggcttatcca gtgcgtttac ccacggcacg
      721 ccgggggaaa tggttgggcc aatatgaaac ccttttatgc ccaggctctg tgtgactacg
      781 ccggaggtct caccacctgc gacaataaag cgtgtcatgc cttccgctgc taaccgcgcc
      841 gctagttgag aaaacagagt ttctactgcc tgactggctt tttgtgcacc gtattgctgt
      901 tgaattgctg ccaatgcgtc agtgctggcg gtggcaaaaa ccagtggagc aagtacactt
      961 tcctggccca gaacccactc tgccagttcg tgtgcataag cggccagagt ttcggttgag
     1021 aggcagcgtg ccacatcaac ttcacgggct ggtgcaattt gacggtaatg tgccacctag
     1081 cggttggtca tttgagagca tgaaccggag agcactacgc cgcgcccagc gagcggacgc
     1141 cctgcttcgc gagcctggtt accgttttct tgtgcccact gccgggccag gccaatcgcc
     1201 agaccagaac cgcccgttac cagtggggca tcgcgcaagg cttctccctg aatttccaga
     1261 tggtgttcgg tcagcgcatc aagcaccgcg tagcggtagt cctcttgctg taagcgagcc
     1321 agctcttgac gaacggcatc cacaccttgt tcgaaaacat gtgccgaaac gacgccgcag
     1381 cgccctgtgg attgcgcttc aaccagacgg ggaagatagc tgttggtcat gggatttacc
     1441 gggtgatggc gcatcccgga ttcggccagc agttgattca ttacgaaaaa atacccctga
     1501 taaaccgtac gtccgttgac cggcagggcc ggagagaaga ccgtaaacgg cgtgtcgaga
     1561 gcatccatta atgcatcggt aaccgggcca atattacctt tcgccgtact gtcgaaagta
     1621 gagctgtatt tgaaatagat ctgtttgcaa ccttgctgtt gcaaccagct cagagccgcc
     1681 agcgattgct gtgtggcttc aaccaccgga caggagcgcg ttttcaggct gatcaccagt
     1741 gcgtcgattg cttccggcat tttacctgtt ggaacaccgt taatttgtac cgttggtaga
     1801 ccgttttcca ccagaaaact ggcgatatcc gtcgcgccgg taaaatcatc ggcgataacg
     1861 ccaatcttga tcatgatttc gctcccggta gagtgatgcc agagaaaatc ttgataactg
     1921 cgctatcgtc ttctttcccg taacccgcgt tactggcgct ggtgaacata ttcaatgctg
     1981 ttgaggccaa tggcagcggg aagtgcaggg ctttggctgt atcggcaacc agaccaagat
     2041 ccttaacaaa aatatcgacg gctgaatgcg gggtgtaatc gccatccacc acatgacgca
     2101 tccggttttc gaacatccag gaatttccgg cggcattggt cacgacgtca tacatcacat
     2161 ccagcgggat ccccgcacgg gctgcaagtg ccatcgcttc ggctccggca gcaatatgta
     2221 cgcccgctaa caactggtga ataattttta cggtcgaacc tagtcccggt tctgcaccta
     2281 tgcgataaac ttttccggca acggcttcca gcacgggtgc cagtcgttca aaggcaatat
     2341 cgctaccgga ggccatgaca gtcatttcac cgttagcggc ttttactgca ccaccagaaa
     2401 ctggcgcatc cagcatttcc agatcgaatc cagccagagc ggtagcaatt tcttgcgcat
     2461 cagcactagc gatagtggaa gaaaccatta ctgccgtacc gggtttcaga tgttgtgcaa
     2521 cgcctgtttc accaaacagc acctgtttaa cctgggccgc attgaccacc agcaccagca
     2581 gagcgtccag tttttcggca aacgtcgcgg cgttatcaga aaccccgcaa gcacctgcct
     2641 ctttcaacgt agcgcaggca ttgctgttca ggtctgcgcc ccaggtagaa aaacctgcgc
     2701 ggacacatga cagtgctgct cccattccca ttgaccctaa gccaacgata ccgacatgaa
     2761 actcagatcc cgttttcatc tgctctcctt gttaatttaa gtgatatttt gtttgacatt
     2821 gtgaatataa gcgctgggag ataacgacat ggtgagctga ttcacataaa ttaacatttt
     2881 gtgttatttt atgtgaacta agcgttagtt gcgccgcgca cgtttcgcag gcaaatagcg
     2941 tagaatgtca gcaggacaaa ggaaggagca aaagttgata cccgtagagc gtcgccaaat
     3001 catccttgag atggtagctg aaaaaggcat tgtcagtatt gctgaactaa cggacagaat
     3061 taatgtgtca catatgacca ttcgtcggga tttacaaaaa ctggagcagc agggagccgt
     3121 tgtgctggtg tccggaggcg tccagtctcc gggacgcgtg gcgcatgaac cttctcatca
     3181 ggtaaaaact gcgctggcaa tgacgcaaaa agcggctatt ggcaagctgg cggcaagtct
     3241 tgttcagccg ggaagttgta tctatctgga tgcgggaacg accacgttag cgatagcaca
     3301 gcatctgatt cacatggagt cactgactgt ggtcacaaac gatttcgtta ttgcgaacta
     3361 cttgctcgac aacagtaatt gcacaattat tcacactggc ggtgcagtgt gtcgggaaaa
     3421 ccgttcctgt gtcggggaag ccgctgcgac catgctgcgc agcctgatga ttgatcaggc
     3481 ttttatttct gcatcgtcat ggagtgtgcg ggggatttct acgccagcgg aagataaagt
     3541 cacggtgaaa cgggcgattg ccagtgctag ccgccagcga gttttggtct gtgatgcgac
     3601 gaaatatggt caggtggcga catggctggc gttaccatta agcgagtttg atcagattat
     3661 tacagacgac ggtttgccgg agagtgccag tcgcgcgctg gcgaagcagg atctctcttt
     3721 gctggtagcg aaaaatgaat aatggcctgc aataacatct ggttactcat gcttcacaga
     3781 agaagcatga gactacctta ttttataaaa tgacagccgc ccacttttcg gcgatccggt
     3841 atcaatataa atctggttag cgaacgtctg aatgttatca aacatcatat gtccaaatat
     3901 aaaataatca gcgccgttta tttgttgtaa ctcgccatta agcgatttct gcacacgata
     3961 aacaggccag agtaattcgc tctccgctat ttctttgcca aagagatatt catcacccgg
     4021 ataatctgca tgtgcgatga catattttat tatgtcgtta gtgatttcaa taatatgtgg
     4081 aaggtgatgg aatttcagca acagatctat tgcctcttgt tgcaatcatt taaatcgaaa
     4141 aactagtcac caccgctggc aagccacata ttgccatcgc cggtcgcgaa cgcatccagc
     4201 gccattgctt cgtggttgcc tttaacggag ataaaccagg gctggtttag caggcgcagg
     4261 acgtcaagac tctccggccc acgatcaata ttatcgccga cagaaataag taagtcggtt
     4321 tcggggaaaa aagagagttg atgtaagcgg gattgtaata actgatattc accatgaata
     4381 tcaccaacga cccatatatg gcgatagtga tgggcattga ttttttgata gcgtgtagat
     4441 ggcatggttt taccctgtaa aataagcttt cctattatac agggtatttt tatttgattc
     4501 gtcagttgtc gttaatattc ccgatagcaa aagactatcg ggaattgtta ttacaccaga
     4561 ctcttcaagc gataaatcca ctccagcgcc tgacgcggag tcagtgaatc cgggtcgaga
     4621 ttttccagtg cttcgaccgc aggcgaagtt tcttctggca ctgacagcaa agacatctgc
     4681 gtaccatcca cttgcgtcgc ggcggcgttc ggcgaaatgc tttccagctc acgcagtttt
     4741 tgccgtgcgc gcttaataac ctctttcggt acgcccgcca gagcagcaac cgccagaccg
     4801 tagcttttgc tcgccgcgcc gtcctgcacg ctgtgcataa aggcgatagt gtcgccgtgc
     4861 tccagcgcat cgagatgtac gttggcgacg ccttccattt tctccggtag ctgggtcagc
     4921 tcgaaatagt gggtggcaaa cagcgtcaat gccttaatct tattcgccag attttctgcg
     4981 cacgcccacg ccagcgacag accatcgtag gtggacgttc cacgcccgat ctcatccatt
     5041 aacaccagac tgtattcggt ggcgttatgt aaaatattgg cggtttcagt catctccacc
     5101 ataaaggttg agcgcccgga agccaggtca tctgccgcgc ctacgcgggt aaagatgcga
     5161 tcgataggtc caatctcgac tttttgtgcc ggtacatagc tgccgatgta ggccatcagc
     5221 gcaatcagtg cggtctggcg catataggta cttttaccgc ccatgttcgg gccggtaata
     5281 atcaacatac ggcgctgcgg cgacagattc agcgggttag cgataaatgg ctcattcagt
     5341 acttgttcaa ctaccggatg gcgaccttcg gtaatgcgaa tgcccggttt atcaatgaag
     5401 gtcgggcagg tgtagttcag ggtataggcc cgttccgcca ggttaaccag cacgtcgagt
     5461 tccgccagcg cgctcgcgct ctgttgcaac gcttccagat gcggcaacag caggtcgaac
     5521 agctcttcat aaagctgttt ttccagtgcc agtgctttgc cttttgaggt gagaacttta
     5581 tcttcgtact cttttagctc tggaatgatg tagcgctcgg cgtttttcag cgtctggcga
     5641 cgcatgtagt tgatgggtgc cagatggctt tgcccacggc tgatttgaat gtagtagccg
     5701 tgcaccgcat taaagccaac ttttagcgtg tccaggccgg tacgttcacg ctcgcggact
     5761 tccagacgct ccagataatc ggtcgcgccg tcagccagcg cgcgccactc atccagctct
     5821 tcgttatagc ccgatgcgat aacaccaccg tcgcgtacca gcaccggcgg tgtgtcgatg
     5881 attgctcgct ccagcagatc gcgcagctcg gcaaactctc ccatcttctc acgtagcgcc
     5941 tgtaccggtg cactatcgac agtttctaac tgcgcacgca gctccggcag ttgctggaaa
     6001 gcgtggcgca tacgggccag atcgcgtggg cgagcagttc gtaaagccag acgtgccaga
     6061 atacgttcca ggtcgccgac ctgacgcagt accggctgca actcggcggt gaaatcctgc
     6121 aatgcgccaa tagtttgctg gcgctcaagc aacacgcgag tatcgcgcac tggcatatgc
     6181 agccagcgtt tcagcatacg actacccatc ggcgtgacgg tgcagtcgag cacagaagcc
     6241 agcgtatttt ccgcaccgcc cgccaggttc tgagtaattt ccaggttacg acgtgtcgcg
     6301 gcatccataa tgatgctgtc ctgctcacgt tccatggtga tagaacgaat atgcggcagg
     6361 gtcgtgcgtt gggtatcttt cgcatactgc aacagacaac cggcagcaca aagtccgcgc
     6421 ggcgcgttct cgacgccaaa accgaccaga tcgcgggtgc caaattgcag attcaactgc
     6481 tggcgcgcgg tgtcgatttc aaactcccac agcgggcgac ggcgcaggcc gcgacggcct
     6541 tcaatcagcg acatctcggc gaaatcttct gcatacagca gttccgccgg attagtgcgt
     6601 tgcagctctg ccgccatcgt ttcgcggtcg gccggttcgc tcaggcgaaa acgcccggag
     6661 ctgatatcca gcgtcgcgta gccgaaacct ttgctgtcct gccagatagc cgccagcagg
     6721 ttgtcctgac gctcctgtaa cagggcttca tcgctgatgg tgcctggcgt aacgatacgc
     6781 acaactttgc gctcaaccgg acctttgctg gtcgccggat cgccaatttg ttcgcagatg
     6841 gcaacggact ctccctgatt caccagtttg gcgagatagt tttccaccgc atggtaggga
     6901 atccccgcca tcgggatcgg ctctcccgcc gaagcaccgc gtttggtcag tgaaatatcc
     6961 agcagttgcg acgcgcgttt tgcgtcgtca taaaacagtt cataaaaatc acccatccgg
     7021 taaaacagca ggatctcggg atgctgggct ttcagcttga gatactgctg catcatgggc
     7081 gtatgggcgt cgaaattttc tattgcactc atggggttat gtccggttcc ctgatattaa
     7141 atggggtgtg atggttttta ttttcgttgt aatcacataa ggcgcacatg ataccggagt
     7201 taatcatttc aggggaagaa gcgaacggcg agttcttgat gctggttccg ggaaagatgc
     7261 tgttttgcag cttttgcgtg atgtttatga agcaagtacg caaaattgta tcgattggcg
     7321 aacccttctt ctttagagtc atagtgccct ttaagattaa gatgtaatat aaatgcatct
     7381 taatgaggtg gaggcagcat gtccggcaag cgtatctctc gtgaaaaact gacgattaaa
     7441 aaaatgatcg atctttatca agcgaaatgc ccgcaggcgt cagcggagcc ggagcattac
     7501 gaggcgttgt ttgtttacgc gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa
     7561 ccagcctgta agcagtgtcc ggtgcactgt taccagcctg caaagcgtga agagatgaaa
     7621 cagattatgc gttgggccgg gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt
     7681 catctcatcg atgacaagcg tccggtgcca gaactgccgg aaaaatatcg tccgaaaaaa
     7741 cctcgtgaat agtatgccgg acaggcagat ctgtccggca atttgcagtt aaatcaatgc
     7801 cgatttatca attcccagcc gcttcatccg tgacagcagg gtcgtgcgtt tcagccccag
     7861 acgttgcgca gcgcctttag gcccggcaac cacgctgttg gtttctttca gcacgcgcac
     7921 aatcaactga tattcatctt cgccctcctg ggcgacaacc gttgcggcag gcggcgtttc
     7981 aggctccggt aaagcaatat ctggcaatga cagctgcagc acgttgccgc gtgttagcaa
     8041 tactgcgcgc tcaatgacgt tttccagttc gcgtacgtta cccggccact ccatgttgct
     8101 caaggtgcgc agcgtctcgg caggaatgct gtcgatattg cgccccagac gacgggcaat
     8161 tttgaaggta aaggctttcg ccagcagcgg aatatcttcc ggacgctcgc gtagtggcgg
     8221 caggtgaatc gggaatacgt tcaggcggta atagagatcg ctacggaact ctcggtcggc
     8281 gaccattttt ttcagatcgc ggttagtcgc ggcaattaaa cgtacgtccg tctgaatgat
     8341 tttgttgctg ccgagacgtt caaactcctg ttcctgcaat acacgcagca acttcggctg
     8401 taactccagt ggcatatcgc ccacttcgtc gaggaacaga gagcttttat ccgccagttc
     8461 aaaacgacca atgcgctggg cgctggcacc ggtaaaagcc ccacgctcat gaccaaacag
     8521 atcgctttcc agcaatccgg caggcatcgc cgcgcagttc attttgacca tgcggcgatt
     8581 attacgccca ctgagattat ggatcgcacg ggcaatcagc tctttacccg tgccagtttc
     8641 accgaggatc agcacggtac tgtcactttg cgccaccatt tcaacttgtt taagcacgct
     8701 gtacatggct tcgctgcggc caataatctc gccaaattca ctatcaacat tgttgagctg
     8761 ctcggtcagg gcgaggtttt catcaaccag ccgttctttc agacgatgga tttcctgata
     8821 ggcgagggcg ttatcgacag cgattgccac acgttcggca atctggcgca gcaaattcag
     8881 attggtagtg gtaaacactt tctcttcgca ttgcgccagt ttcagcacgc ccagcatggt
     8941 gtcgccagac atcagcggta acaggcacaa ggtttgaatc tggttgcccc aggtgtcgaa
     9001 caacatgcgt tcataggggg ctaaatcgtc ccgctcgtag agattgatca gcagcatctc
     9061 tttacttttg aacacgcgtt cggtgagggt tccggcttca tcgacttcgc tctgttcgtg
     9121 ggcgggatgt tgtttatcaa gatagtgagt ggagtagatg ttgagtttgt ttttacggtg
     9181 gctgcgtaag acaatgctga tatcgtcaat gtcgaaatag taatggattt ctttggcgac
     9241 ttcgctgacc agttcgtcca tatccaggcg ggaaagcacc gcgttggtaa tggcaaccag
     9301 gatgcggaag ttatcgcgct cccggcataa caactcatag tcgacattgt tgacgacgcg
     9361 gctctggatt tgttcggtga cgacagaaac gatctgcgta aatgtttgca gacgattgaa
     9421 ctctttttcg ctccagggac gatcgtcata acgaataaat tcacagccac caaaaatatg
     9481 ccctcccgcc gccagtggca tcaggcaata gtgaccaaat ttggggtata gcccacctgc
     9541 ggccagctgc ggccaggttt cacaaaattc ttcgtaactg caatgcagcg tatcagggcg
     9601 cgacaaaatg ctgcgtaccg gaccgtgtgc cagaacagtt tcgtcttcat atttaatggg
     9661 ggtgtctttt tcacgcgacg cgtaataaga cgcacgttga gtctgcgctt gccacaacac
     9721 aatagccgcg ttgtcggcga gcgcagaacg ctttaccagt tgcgaaagag cctcacacag
     9781 cgaggccaga tcgggctgct gcaatagtgt ccgagtgatg tcgaacaacc cttgctgtcc
     9841 gagaccactc atcggtgtat atgacatttt gttcttccag gaaagcaccg caaagcggtg
     9901 caaaaggtca ttgactaact ataggagatt tagaatttta ttagcatata cgcggaagcg
     9961 gttcggcgtg tggtaaatcg agggttcgtt tcacgccata cagaccggca agacgaacac
    10021 ctttacgttc caccacttca ccaatcagcg ccgcgtcttt ccccagtgga tgggaatgta
    10081 acgctgccag cacttgctct gccgcgttgc gttcaacggc gatcaccagt ttaccttcgt
    10141 tggcaaagtt cagggcgtcc agtcccagca attcgcaaac gccgcgcacg gcaggtttaa
    10201 ccggcagcgc tgattcagaa atttcaatac cacaaccgca ggctgccgcg aactcatgaa
    10261 ccaccgcgtt tacaccacca cgggtggcat cacgcagcgc tttcacgccg ggaatgtcac
    10321 gcagcgtctg aataagcggc gtcagcaccg cgcagtcgct gaccagttcg ccatccagcc
    10381 ccagctgctc acgcaggtta aggatagtcg ccccgtggtc gccgagtgta ccgctaacca
    10441 gcaatacatc gcctgcggtt agcgtctgtg cgccccagtg aatattcgcc ggaattgcgc
    10501 ccatgccagc ggtgttgata aacagtttat ctgccgcgcc gcgctgcacc actttagtat
    10561 cgccagtaac gatggcaatg cctgccgtgc gggcggtttc tgccatgctg gtcactgcgg
    10621 ctttcagtgt ctccatcggc aatccttctt cgaggataaa gccacaggag agatagcgcg
    10681 gaatagcgcc actgaccgca acgtcattcg cggtgccgca aatcgccagc ttgccgatat
    10741 taccgccagg aaagaacagc gggtcaatga cgtaactgtc ggtggagaac gccagacggt
    10801 cgccttccgc taccagctgc gccagatcaa gacgtgcctg atcttcctgc tctgccagcc
    10861 acgggttggc aaaggcttcc ataaacaggc tgttgattaa ttgctgcatc gcctggccgc
    10921 cgctgccgtg ggcgagttgg atattattca cgcttcactc tcctgctgac gatactgata
    10981 ccacgcggcg cacgctcctt cggaggagac catcagtgca ccaaatgcgg tttgaggatt
    11041 acaggtgtta ccaaacagcg ggcattgatg cggcttacat ttgcccgtta atacctcacc
    11101 acaacgcgcg cgcgggtcat cgcagacctg ctgcggtgcc gggcggaaat gtgcttcggc
    11161 atcgaatcgt tgataatccg gcgtcaggtg cacgccagaa gattcaatca cgcctaagcc
    11221 gcgccattcg ctgtcgccgt tgacacagaa cacatcggca atcgcctgtt gcgccagcag
    11281 gttaccggca tccggtacca cccgacgata ctgattctct accttgctgt gggccgctat
    11341 tttctgctcc accagcatga ccacgccttg cagtagatca aggggttcga aaccagcgac
    11401 caccagcgga cgatgaaaat cgctggcgat aaaattatag gcatcagtgc cgataaccat
    11461 actaacgtgg cccggcgcga ggaacgcgtc gataccgtta tccggctgtt ccagcaaact
    11521 gcgcagcgtc gggataagcg taatgtgctg gcagaagaag taaaaattct gcacatcacg
    11581 cgctttcgcc tgttgcagag tgatggcggt ggtcggcatg gtggtttcaa aacctaagcc
    11641 gaagaacacc actttgcggg ttggattctc ctgcgccagt ttcaacgcat ccatcggcga
    11701 gtaaacgatg cgcacatcgg caccgcgtgc ttttgcctgc aacagcgatc cctgtttccc
    11761 tggtacgcgc atggcgtcgc caaaggtaca gaagatgact tccggatggc tggcaatctc
    11821 cacgcaggtg tcgattctac ccatcggcag tacgcacacc gggcaacccg gaccgtggat
    11881 aaactcaacg ttttcaggca gtaactggtc gaggccgaat ttaaaaatgg cgtgggtatg
    11941 accgccacac acttccataa tccgcagagg gcgttcggcg gtgtaagaga gatgtgaagc
    12001 acgttcgcgc agatgctcaa ttaactgcat cacctgttcc ggcgcgcgat attcatcaac
    12061 aaaacgcatt atttttcctc gccatacaac agcgcaccga catccggctc aacgtcaaac
    12121 atgttttgta aggcgtcgag agtgtcgcgt gcttcggctt cattaattac gctcatggca
    12181 aagctaacgt gaaccagtac ccactggccc acgcgcggct gaccgttttc atcgcagctg
    12241 ccgactaacg ttaaatcgac atcgcgctga atgccgcaga cgtcgacttt cgcctggttg
    12301 ccgtcaatgg tgcggatctg gccgggaacg cctatgcaca tcgctgtgtc tccagccagt
    12361 tcagccactg gtccatccct tcgccgctgg tggcggaaat aaggatgatt tcaatttctg
    12421 gattgacttc gcgggcgcag gcgatgcact tctcaacgtc aaagttgaga tacggcaaca
    12481 ggtcaacttt gttgagcagc atcagcgagg cggcggcaaa catatgcgga tatttcagcg
    12541 gtttgtcttc accttcggta acggaaagca ccgctacttt gtgtttttca ccgagatcga
    12601 agctggccgg gcatacgagg ttgccaacgt tttcgataaa cagaataccg ttatcgtcca
    12661 gtggcagacg cggtgcggcg tcggcaatca tctgtgcgtc aagatggcag cctttaccgg
    12721 tgttcacctg aatcgctggt gtaccggtag cgcgaatgcg tgcggcatcg ttcacggttt
    12781 gctggtcgcc ttcaataacc gcgcacggaa cgctgtcttt caggcgcatt aaggtttccg
    12841 tcagcagggt ggttttaccg gaaccagggc tggaaaccag gttgagcacc agttgcttgc
    12901 gggcagcaaa gcgcgcgcgg ttgcgttcag ccagacggtt gtttttgtcc agcacgtcaa
    12961 tttcgacttc cagcatccga cgctggctca tgcccggtgt gtgagtgccc gcttcgccat
    13021 gaccgtaatg caggtcgcct tcttcagtct ggctgggggt aaattcaggc gctttaatgc
    13081 cggtgatttt catctgcggg cgtgccgccg gggcaaatgg cgcgctacga aacgcggaat
    13141 gagggttatg ttcatcaccc tcgatataca ggttgccttc accgcaaccg catgttgtac
    13201 acatcgctca ctcctggtct atttctatcc gccgaatctg taaaccgtcg tctgccacaa
    13261 tctgtagcat gtcaccatga cactgtggac agcggcggac gcgctgggtc agtagcgtca
    13321 catactgttg gcatgtttca caccagcatt cggcctcttg ttcttcgagg tgcagtttac
    13381 aaccttccgc cacgctgccg cggcaaacca gatcaaaaca aaaggcaaga gagctggttt
    13441 cgacacaaga aaatgcgcca attttgagcc agaccccagt tacgcgtttt gcgccgtgtt
    13501 ttgcggcctg ctgttcgatc aattccagtg cccgttggca gagggttatt tcgtgcatat
    13561 cgcctcccat taactattgc cagctacaag caataattgc gccagtgttg attatccctg
    13621 cggtgaataa tgtcgatgat gtcgaaatga cacgtcgaca cggcgacgaa attcatcttt
    13681 agcttaaaaa tctctttaat aacaataaat taaaagttgg tacaaaaaat gcttaaagct
    13741 ggcatctctg ttaaacgagt aacctgacaa tgactatttg ggaaataagc gagaaagccg
    13801 attacatcgc acagcggcat cgtcgcctac aggaccagtg gcacatctac tgcaattcgc
    13861 tggttcaggg gatcacgtta tcgaaagcgc gcctgcatca cgccatgagc tgcgcgccgg
    13921 acaaagaact ctgtttcgtc ctttttgaac attttcgtat ttacgtcacc ctggcggatg
    13981 gctttaacag ccacaccatc gagtattacg tcgaaacaaa agatggcgaa gacaaacagc
    14041 ggattgcgca ggcgcaactg agcattgacg gcatgattga tggcaaggtc aacatccgcg
    14101 atcgcgaaca ggttctggaa cactatctcg aaaaaatcgc tggcgtttac gacagcttat
    14161 acaccgccat tgaaaacaat gtgccggtga atttaagcca actggtaaag ggacaaagcc
    14221 cggcagcatg agctgaggct ttgcccgttt tgcaggcgtt acgcctgttt ggggatgggc
    14281 gtgtcgatga gtgtcgaaaa tgacatttca tcggcatgtt ttcgtcaaaa atgacaatca
    14341 cctgaggaat gcctggtgaa tcgttttgta attgccgact ccacgctctg tatcggctgc
    14401 cacacctgtg aggccgcctg ttcagagacg catcgccagc acggcctgca atcaatgccg
    14461 cgcctgagag tgatgctcaa tgaaaaagaa tctgcgccgc tgctctgtca ccactgtgaa
    14521 gatgcaccct gcgcggtggt ctgcccggtt aacgccatca cccgcgttga tggggccgtg
    14581 cagttgaatg aaagtctgtg cgtaagctgc aaactgtgcg gcatcgcctg cccgtttggc
    14641 gcaattgaat tttccggcag ccgtccgctg gatattccgg caaacgccaa taccccgaaa
    14701 gcgccaccgg caccgcctgc tccggcgcgt gtcagcacat tgcttgactg ggtgccaggt
    14761 attcgcgcga tcgccgtcaa atgtgacctt tgtagctttg atgaacaagg tccggcctgt
    14821 gtgcggatgt gcccgactaa agccctgcat ctggtggata acaccgatat cgcccgcgtc
    14881 agcaaacgta agcgtgagct gacttttaac acggactttg gcgatctcac cttgtttcag
    14941 caggctcaaa gtggagaggc taaatgagcg caatttccct gatcaatagc ggcgtggcat
    15001 ggtttgtcgc cgccgctgtt ctggcatttc tcttttcttt tcaaaaagcg ctaagtggct
    15061 ggatagccgg aattggcggc gcggttggta gtctgtatac ggcagccgcg ggcttcactg
    15121 tactgactgg ctcggttggc gtgagcggtg cgatgtcgct ggtaagctac gatgtgcaaa
    15181 tctccccgct taacgcgatt tggctgatta ccctcggcct ttgcggtctg tttgtcagcc
    15241 tctataacat tgactggcat cgccacgcgc aggtgaagtg caacggcttg cagatcaata
    15301 tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa cctcggcatg ttcgtggtaa
    15361 tggccgaagt catggccttg tgtgcggtgt tcctcaccag caacagcaaa gagggcaaac
    15421 tgtggtttgc gctggggcgt cttggcactc tgctgctggt gattgcttgc tggctgctgt
    15481 ggcagcgtta tggcacgctg gatctgcgcc tgctggatat gcgtatgcaa cagctgccgc
    15541 tcggttccga tatctggctg ctcggttccg atatctggct gctcggtgtg attggctttg
    15601 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
    15661 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
    15721 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
    15781 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
    15841 gcctgctggc ttaccacacc ctggaaaata tcggcattat cctgctag
//
LOCUS       DADLXX010000099        15082 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLXX010000099 DADLXX010000000
VERSION     DADLXX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.100"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            29..292
                     /locus_tag="JNX27_002940"
     CDS             29..292
                     /locus_tag="JNX27_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7884075.1"
                     /translation="MSKLESLPTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLV
                     FAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            302..940
                     /locus_tag="JNX27_002941"
     CDS             302..940
                     /locus_tag="JNX27_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7884076.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            943..2106
                     /locus_tag="JNX27_002942"
     CDS             943..2106
                     /locus_tag="JNX27_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7884077.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2190..3814
                     /locus_tag="JNX27_002943"
                     /pseudo
     CDS             2190..3814
                     /locus_tag="JNX27_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="JNX27_002944"
     CDS             complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="JNX27_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7884078.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="JNX27_002945"
     CDS             complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="JNX27_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7884079.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4866..5615
                     /gene="yjfP"
                     /locus_tag="JNX27_002946"
     CDS             4866..5615
                     /gene="yjfP"
                     /locus_tag="JNX27_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7884080.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="JNX27_002947"
     CDS             complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="JNX27_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7884081.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="JNX27_002948"
     CDS             complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="JNX27_002948"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7884082.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCANDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7894..9291
                     /gene="ulaA"
                     /locus_tag="JNX27_002949"
     CDS             7894..9291
                     /gene="ulaA"
                     /locus_tag="JNX27_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7884083.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9307..9612
                     /gene="ulaB"
                     /locus_tag="JNX27_002950"
     CDS             9307..9612
                     /gene="ulaB"
                     /locus_tag="JNX27_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7884084.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9622..10086
                     /gene="ulaC"
                     /locus_tag="JNX27_002951"
     CDS             9622..10086
                     /gene="ulaC"
                     /locus_tag="JNX27_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY7884085.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10100..10750
                     /gene="ulaD"
                     /locus_tag="JNX27_002952"
     CDS             10100..10750
                     /gene="ulaD"
                     /locus_tag="JNX27_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7884086.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10760..11595
                     /locus_tag="JNX27_002953"
                     /pseudo
     CDS             10760..11595
                     /locus_tag="JNX27_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
     gene            11595..12281
                     /locus_tag="JNX27_002954"
     CDS             11595..12281
                     /locus_tag="JNX27_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7884087.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12410..12685)
                     /locus_tag="JNX27_002955"
     CDS             complement(12410..12685)
                     /locus_tag="JNX27_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7884088.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13012..13407
                     /gene="rpsF"
                     /locus_tag="JNX27_002956"
     CDS             13012..13407
                     /gene="rpsF"
                     /locus_tag="JNX27_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7884089.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13414..13728
                     /gene="priB"
                     /locus_tag="JNX27_002957"
     CDS             13414..13728
                     /gene="priB"
                     /locus_tag="JNX27_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7884090.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13733..13960
                     /gene="rpsR"
                     /locus_tag="JNX27_002958"
     CDS             13733..13960
                     /gene="rpsR"
                     /locus_tag="JNX27_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7884091.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14002..14451
                     /gene="rplI"
                     /locus_tag="JNX27_002959"
     CDS             14002..14451
                     /gene="rplI"
                     /locus_tag="JNX27_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7884092.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14522..15079)
                     /locus_tag="JNX27_002960"
     CDS             complement(14522..15079)
                     /locus_tag="JNX27_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7884093.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3529 a         3671 c         4058 g         3824 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ctacattgtt
       61 aggttacgtt atcccattat ccagtgcggc aatcaatgcg gtgagtattc cagactattt
      121 cgcctggggc gggattgcac tggtgattca gttactcgtt tttgctggtg tcagacttta
      181 tatgcctgca ttaagcgaaa aaattattaa tcacaatacc gcagcaggaa tgtttatggg
      241 aaccgccgcg ctggctggcg gtatttttaa cgcagcttgt atgacatggt aatggacgat
      301 catggccaga aaacgcaaat caagaaataa cagtaaaatt ggtcacggag cgatcagtcg
      361 cattggtaga ccgaataatc cttttgaacc gcgtcgcaat cgctacgcac aaaaatactt
      421 aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgta gcgatagtag
      481 cgatgtcgat aacgacggcg acggaacgtt ttacgcgaca gtgcaggatt gtattgatga
      541 cggtaataat gccgacattt gcgcgcgtgg ctggaacaac gccaaagcgg cattttatgc
      601 cgatgttcca aagaatatga ctcagcagaa ctgtcagtct aagtacgaaa attgctacta
      661 cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa gccgggttat
      721 tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat ttgcttcccg
      781 ccccgtctgg cgcaacactt ccggtgatta ctcctggcgc ttcggttctg gcaaaaaaga
      841 gtcttactct tcaggcggct ttaccaccag aaaagcgtcg accgtttcgc gcggcggcta
      901 tggtcgttct tccagcgccc gtgggcattg gggaggctaa tcatgctgag acacaacgtt
      961 cctgtgcggc gggatctgga ccagatcgcc gctaacaacg gtttcgactt tcatatcatc
     1021 gacaatgaaa tctattggga cgagagtcgg gcttaccgtt ttactctgcg ccagattgaa
     1081 gagcagatcg aaaaaccgac tgcggaactg catcagatgt gccttgaggt ggtggatcgc
     1141 gcggttaaag acgaagagat cctgacgcaa ctggcgatcc cgccgttgta ctgggatgtg
     1201 atcgccgaaa gctggcgcgc acgcgatcct tcgctgtatg gccggatgga ttttgcctgg
     1261 tgtggcaatg cgccggtgaa gctgctggag tacaacgccg atacgccgac ctcgttgtat
     1321 gagtcggctt atttccagtg gttgtggctg gaggatgccc ggcgcagcgg cattattccg
     1381 cgtgatgccg atcagtacaa cgccattcag gaacgcctga tttcgcgctt tagtgagctt
     1441 tacagtcggg aaccgtttta tttttgctgc tgtcaggaca ccgatgaaga caggagtacc
     1501 gtgctgtact tgcaggactg cgcccagcag gcagggcagg agtcgcggtt tatctacatt
     1561 gaagatctcg gtttgggcgt cggcggcgta ctgaccgatc ttgatgataa tgtcatccag
     1621 cgtgcattta agctgtatcc gctggagtgg atgatgcgtg acgataacgg tccgctgctg
     1681 cgcaagcgtc gcgagcaatg ggtggagccg ttatggaaaa gtatcttgag taataaaggg
     1741 ctaatgccgc tgctttggcg cttcttccct ggtcatccta accttcttgc atcctggttt
     1801 gagggtgaaa aatcgcagat tgccgctggc gaaagctatg tgcgtaaacc gatctactcg
     1861 cgcgaaggcg gaaacgtcac cattttcgac ggtcagaata acgtcgttga ccacgctgat
     1921 ggtgattacg ccgatgaacc gatgatctac caggcgtttc aacctctgcc gcgatttggc
     1981 gatagctaca cactcatcgg tagctggatt gtcgatgatg aagcgtgcgg aatggggatc
     2041 cgtgaagata acacactgat caccaaagac acctcacgtt tcgttccgca ttacattgct
     2101 ggataagaat gttttagcaa tctctttctg tcatgaatcc atggcagtga ccatactaat
     2161 ggtgactgcc attgatggag ggagacacag tgcactggca aactcacacc atttttaatc
     2221 aacctatacc attaaataac agcaacttat acctgtctga tggcgcgctt tgcgaagcgg
     2281 taacgcgtga aggtgctggc tgggatagcg attttctagc cagtattggt cagcagttag
     2341 gaacggctga atcccttgaa ctggggcggc tggcgaatgt aaacccgcct gaactgttgc
     2401 gctacgatgc ccaggggcgg cgtctggacg atgttcgttt ccatcccgca tggcacctgc
     2461 tgatgcaggc gttatgtacc aatcgggtgc acaatcttgc ctgggaagaa gacgctcgct
     2521 ccggcgcatt tgtggcgcgc gcggcgcgtt ttatgttaca tggcaggttg aggctgggtc
     2581 gttatgtcca ataacgatga cctttgccgc cacgccattg ttgttacaga tgttacccac
     2641 gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc acttattgcc
     2701 aggtgggcaa aaacgcggtt tgttgattgg catgggaatg acggaaaagc agggcggttc
     2761 cgatgtcatg agcaacacca tccgtgcaga gcgtttggaa gatggctctt atcggctggt
     2821 ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc tggcgcagac
     2881 cgcgggcggt ctgtcctgct tttttgtgcc gcgctttttg cctgacgggc aacgcaacgc
     2941 gattcgcctc gagcggctga aagataagct gggtaatcgc tctaacgcca gttgcgaagt
     3001 ggagtttcag gatgccattg gctggttatt ggggcaggaa ggggaaggaa ttcgtctgat
     3061 cctgaaaatg ggggggatga cgcgttttga ttgcgccctg ggtagccatg ccatgatgcg
     3121 ccgtgcattt tcgctggcga tttatcatgc acatcaacgc catgtttttg gtaatccatt
     3181 gatccaacag ccccttatgc gtcatgtctt aagtcgcatg gcacttcagc ttgaagggca
     3241 aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgtgccgatg ccaaagaagc
     3301 cctgtgggcg cgtttattta cgcctgcggc aaaatttgtg atctgcaaac gaggtatgcc
     3361 gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg agagcgagct
     3421 gccgcggctt taccgggaga tgccggtaaa cagtatttgg gaaggttccg gcaatattat
     3481 gtgcctggat gtgttgcgcg ttctcaataa gcaagcgggc gtatacgact tattgtcgga
     3541 agcatttgtg gaagtgaaag ggcaggatcg ctattttgat cgcgcggttc gtcgtttaca
     3601 gcagcagctg cgtaagccag ctgaagaact ggggcgagag attactcatc agctattcct
     3661 gctgggctgc ggtgcgcaaa tgttgaaata tgcttctccg ccaatggcgc aggcgtggtg
     3721 tcaggtgatg ttagatacgc gcggcggcgt acggttgtca gagcagatcc agaatgattt
     3781 attgctgcgg gcgacggggg gagtgtgtgt gtaagcgtat acgactgatg cgacgctggt
     3841 ttcgattaac taaatgaaat atgtgaaaat tgtaggccgg acaaggcgct cgcgccgcat
     3901 ccggcattgt tcatttgctg tagatgacac tcatgcatac agtatcgcct gtacgcgcca
     3961 gttatcaggc tgctggtctt cgtacatggt aataatgcgg taccatgacg caccttgttc
     4021 atcggccttt aacgccacga tacgttccac gtcctgcgga tcccctgaaa tattgctaac
     4081 tgagatctga cctatttcat tcaggcccgt cacgcagtca gcactggcga attctgcaga
     4141 ctgtgccgtg gcgctcgcag gataagtcac taattgcagc gagggtgagg caagtaattg
     4201 tttcatcgtc agctccattt ttcgtttccc cacatgttgc agggcattcc atcgcggtaa
     4261 taaacgccca agcttccagc cggtaattta ttgtgcacag gtaaacgcat agggacgcaa
     4321 agcaatgtaa agctgactat gtaagttgtc tgatttattt acggtacaaa atggcttgtg
     4381 aataccactg tcctgtcacg atggtttcgt ccaccatgac gacaacgtaa taatcagctt
     4441 ttgcagcgac agcttttgct ctgatttctg ctaatgcgtc atccggagaa ccccgaacca
     4501 tcgtgcttac gctacctatt cgctgtaacc cttgcgtctg gtcgcggcga atctcttgcg
     4561 gatggtccgt tactggcggt gctggctgtg gcgtaccttg cagtgcgcta caggcactta
     4621 acatcaacac caataataaa ctggcaaacc ggtaaataac gctattacgt ttcctgctaa
     4681 ccatagtgta gtgccttatt aatttaactt tgggggaatg ttcccgaatt gttacctggt
     4741 acgcgatttt cgaatgaaaa cgtgacgaaa gcgtaaccca catctcaaca ttatgaacta
     4801 ataccgccag cagaaacttg tgctaaggat ttcttcatgt cataccagac aaaaaggaga
     4861 gacagatgat tgaaatagaa tcacgcgagc tggcagatat tcccgttctt catgcttatc
     4921 ctgtcgggca aaaagatacc ccgttaccgt gcgtaatttt ttatcacggc tttacttcat
     4981 ccagcctggt gtatagctat tttgccgttg cgctggcgca ggctggtttg cgggtgatca
     5041 tgccggatgc gcccgatcac ggtagccgtt ttagtggcga cgaagcgcgg cggttaaatc
     5101 aattctggca aatcttgcta caaagtatgc aggaattcac tactttacgt gcggcaatag
     5161 ccgaagaaaa ctggctgctt gatgaccgtc tggcagtcgg tggcgcgtcg atgggcgcga
     5221 tgacggcact gggaattacc gctcgccacc ccacggtgaa atgtaccgcc agcatgatgg
     5281 gatcgggcta ttttacatcg ctcgcccgtt cactgtttcc accgctgata cctgaaacgg
     5341 cagcacagca gaatgaattc aataaaattg tcgcgccgct ggcagagtgg gaagcgacaa
     5401 accacctgga acaacttggg gacagacctc tactgctgtg gcatggcctc gacgatgacg
     5461 ttgtgcctgc cgacgaatca ctacgtttgc agcaggcctt aagcgagacg ggacgggata
     5521 aactgctaac ctgttcatgg cagccaggcg tgcgtcaccg cattacgcct gaggcgttag
     5581 atgctgccgt gacatttttc cgccagcatc tttaaacacg cagaatgctg accccttgcg
     5641 cttccagttg ttgcaggatt tccgggttag catttttgcc ggtgatcagc atatcgattt
     5701 gatcggcacg gctaaaaagc attcccgcgc gttcgccaat cttactgcta tcaaccagta
     5761 ccaccagttt ccctaccacg ctcagcatct tctgctctgc cattgctgtc agcatatcgg
     5821 ttttatacaa cccttctgcg gtcagccctt ttccgctggt aaacatccag tgcccggcat
     5881 agagactgtt ttcgctgccc tgcgggctta aagtgatgga ctgacttttg ttgtactgtc
     5941 cacccataat gatcacgctg tcatgttctt gatcgatcag gtaatttgcc agcggtagat
     6001 aattagtgat gatttgcact ggcttgccac acatttcccg cccaagcaga aacgcggtgg
     6061 agccgcagtt gatgactacg ctttcgcccg gattaaccag ctgcgaggcc gctttagcaa
     6121 tacgtacttt ttcatcgtga ttctgcgcct gatgcagatt catcggcgtc cagcgcgggc
     6181 gctgttgggt aatagcttct gcgccattgc gcaccttttt cagtttgccg ctttcgccaa
     6241 gtttattgat atcgcgtcgc gcagtggcag gcgaaattcc cagacgctca acgactttct
     6301 caacggtcac aaagcccaat tgtgcgagca tttccaggag gatttgatgt ctttgtgctt
     6361 cagtcatgag ctattccgat aaaatttgat ttttttagat gatatttgaa atagccagga
     6421 aatactacgc cggatagcgc gaacttctcc acgctatccg gtataaacga cagattacag
     6481 gaatgacttg aacggcagat ccggttcaat agtgaagcaa tcatcgaaac cgcgcggata
     6541 gtggtactcg aagttgtctt tatccagcgg ccaggtaaat ttgccgccaa cctgccagat
     6601 aaacggcttg aagccatact tcagacgatc ttttttcatc tcccacagca cgcggatctc
     6661 ttgcggatcg gcctggaagt ttgaccagat gtcgtggtgg aacgggataa ccactttcgc
     6721 attcagggct tcacccatac gcagcatatc ggcgctggtc attttgtcgg taataccgcg
     6781 cgggttctca ccatacgatc ctaacgccac atcgatctga tgttcgttac cgtgcttcgc
     6841 ataatagtta gagtagtggg aatcgccact gtgatatagg gaaccgccag gggttttgaa
     6901 caggtagttt actgcgcgat cgtccatgcc atctggcagt acgccagccg ctttttgatc
     6961 ggcaggcagg gtgatcagtg cagtacggtc gaaagcgtca agcgcatgaa tttcaatgtc
     7021 tttcactttt acaacatcgc ccggtttgac cacgatgcaa cgctctttcg gtacgcccca
     7081 gccaatccac aaatcaacgc aggttttcgg tccaatgaat ggtacgtcat tagcacagtt
     7141 ctgcatcacg gcagcagcga cgttaacgtc gatatgatcg ttgtgatcgt gagtcgccag
     7201 taccgcgtcg atctggcgaa tcgcaaacgg atcaagaaca aacggggtgg tgcgtaggtt
     7261 tggctgcagt tttttcacgc cagccatgcg ctgcatctgg tgaccctgtt tcattaacgg
     7321 gttaccgtga ctttgtttgc cagtgccgca ccagaaatca acgcaaacgt tggtgccacc
     7381 ttctgatttc aaccagatcc cggtgcagcc tagccaccac atcgcaaatg tgccaggggc
     7441 gacctgctct tgttcaattt cttcattcag ccagctaccc cactccggga aagtgctcag
     7501 gatccaggat tctcgggtga tacttttcac tttactcatc gccatttacc ttcatgatag
     7561 ttcaattcga atcaatatgt gattggtttt gattaatcct gacactattt tttcaggaag
     7621 gcaatgacca ttttttgact ttttgccagg gaagttgttg ttgattttga gtgtagaaag
     7681 atttaatgga atgtatagat aaattaacta aatgaattta aataaataat tttttcgttg
     7741 tgtgaatccc actctatccg tgtggaatta tttgcgggtc gcgccacatt taatcataaa
     7801 taatcttgtt gtgattactt ttgaaaatta gagtgagtgc acaacattcc gggtgtgcgg
     7861 aatacccggt tacctcttct tcaggagatc gttatggaga tcctctacaa catctttacc
     7921 gtgtttttta accaggtcat gaccaatgcc ccgttgttgc tgggtattgt gacctgtctg
     7981 ggctacatcc tactgcgcaa aagtgtcagc gttattatta aaggcacgat taaaaccata
     8041 attggtttca tgttgttgca ggcagggtcc ggcatcctca ccagcacctt caaaccggtg
     8101 gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa tttccgatac ttacgcgtcg
     8161 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     8221 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     8281 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     8341 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8401 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8461 ttctccatcg gtcaccagca acagtttgca tcattaattg cctataaagt tgcgccgttc
     8521 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8581 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8641 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8701 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8761 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8821 gttctggcga ttgactgtgc ggctatttat agcttcgctc cgaacgccgt ggtctggggc
     8881 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8941 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     9001 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     9061 atcgaaatct ttggttgcgt ctgggtggtg aaacttaccg gtatgagtgc ctggatgggg
     9121 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     9181 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     9241 ctgcgcgcag aagaagatgc agaaaaacaa ttggcagaac agtctgctta ataaggagtt
     9301 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9361 atgaagatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9421 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9481 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9541 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9601 gatgtgaagt aaggacgcgc catgaaatta catgattcgc tggcggagaa taaatccatc
     9661 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9721 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9781 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9841 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9901 gaagataacg acccggtgga tatcctcatc accatggcgg tggtcgatgc caacacgcac
     9961 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
    10021 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
    10081 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
    10141 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
    10201 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
    10261 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
    10321 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10381 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10441 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10501 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10561 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10621 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10681 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10741 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10801 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10861 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10921 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10981 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    11041 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    11101 ccagctgcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    11161 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cacaggtgac gctggcgatg
    11221 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    11281 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    11341 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11401 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11461 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11521 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gcatggccaa agcaggcatg
    11581 gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc
    11641 cgcgctatgg gctggtgacc tttacttggg gcaacgtcag cgctatcgac cgcgaacgcg
    11701 ggctggtggt gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg
    11761 tggtggtcga tatgagcggc aaggtggtgg aaggggagta tcgcccatct tccgacactg
    11821 cgacgcatct cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact
    11881 ccactcatgc caccgcatgg gcgcaggcgg ggctggcgat cccggcatta ggcaccacgc
    11941 acgctgacta cttctttggt gatatcccgt gtacgcgtgg attaagcgaa gaagaggtgc
    12001 agggtgagta tgaactgaac actggcaaag tgattatcga aacgctgggc aacgccgagc
    12061 cgctgcatac gctgggaatt gtggtgtatc agcacggacc tttcgcctgg gggaaagatg
    12121 cccacgacgc ggtgcataac gcggtagtga tggaagaagt ggcgaaaatg gcgtggattg
    12181 cgcgcagtat taatccacaa ctcaatcata tcgacagcta cctgatgaat aaacacttca
    12241 tgcgtaagca cggtcctaac gcttattacg ggcagaagta aaacacgcgc tgcggaaatt
    12301 tccttcctcg ggagataact ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc
    12361 gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc tagcgataaa
    12421 tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga attacgtggt
    12481 agtacgttgc gccttgcgca tctgtttctt catttaatgc ctgacgtgct tcgctttcag
    12541 tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcgtcc atatttctgg
    12601 cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa agcacagcca
    12661 gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt gtgtgctctc
    12721 ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat ggtgaccatt
    12781 tctgatgcag ttgttcaaaa aaacactgtg acggaatgtg atgaacttca aatcagcggt
    12841 ttagaggtta tttgcgaaag gggagattta tttctgctcc gcccttgagt ttagcggggt
    12901 atgtcagtac tataacgcgt catttttcag ccgaccttta acacgttcct tgcctccccg
    12961 ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat
    13021 tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg catgatcgag
    13081 cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga agactggggc
    13141 cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt tctgatgaac
    13201 attgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt caacgatgcc
    13261 gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt
    13321 aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat
    13381 gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt gttgtccggc
    13441 accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca ctgccagttc
    13501 gtgcttgagc atcgttctgt gcaggaggaa gccggctttc accggcaggc atggtgtcaa
    13561 atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat aacggtcggc
    13621 agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg actgagcaaa
    13681 atggttttgc acgccgagca gattgaattg atagattctg gagactagcc atatggcacg
    13741 ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag agatcgacta
    13801 taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg tcccaagccg
    13861 tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca aacgcgctcg
    13921 ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg tccattaata
    13981 cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc aaacctgggt
    14041 agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag
    14101 ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg tcgcgctgaa
    14161 ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga gaaaatcaat
    14221 gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc
    14281 atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt ggctaagagc
    14341 gaagttcgtc tgccgaacgg cgttctgcgt accactggtg aacacgaagt gagcttccag
    14401 gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata attcgttatt
    14461 caacgagacg taaaaacgcc gaccattggt cggcgttttg ctttctattt ttcgtcaggc
    14521 attaatttcg caagtagatc cattcaccat tctcttgtgt ccataagtca ggatggtttg
    14581 ctggcgttag atgcatacga tcactcctta tgtctcggtc ctgcggcact cgctcgaaac
    14641 ctaaacccga gtagattgcg gctgaatttg ataaggaacg tagaccaatt ttcaaatcat
    14701 ggggtaaatc agaatacgcg tttagcaatg cagcaatgat gaatccgagg gcattagaaa
    14761 cgcctgtaac agtgcgaagt tggtcgatat aaagatcgat tgttggtagt tcagatgacc
    14821 agcaattagg catttgggca ggaagggtta caacgtatcc tatcgtttgg ccggtatagt
    14881 ctggagtact ataaccaggt cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt
    14941 cgaaggattc gacatcttgc aataaatatt tcagttctgc ggttaacctc gcacccgcgt
    15001 aagctttatt gtatttctgg gcgtgaatat tatctaatgt agcttcatgg ttccatccaa
    15061 aagaattatc accattcatt gt
//
LOCUS       DADLXX010000100        14935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLXX010000100 DADLXX010000000
VERSION     DADLXX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.101"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            596..793
                     /locus_tag="JNX27_002961"
     CDS             596..793
                     /locus_tag="JNX27_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884094.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(743..1936)
                     /locus_tag="JNX27_002962"
     CDS             complement(743..1936)
                     /locus_tag="JNX27_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7884095.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2072..3796
                     /gene="iucA"
                     /locus_tag="JNX27_002963"
     CDS             2072..3796
                     /gene="iucA"
                     /locus_tag="JNX27_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7884096.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3797..4744
                     /gene="iucB"
                     /locus_tag="JNX27_002964"
     CDS             3797..4744
                     /gene="iucB"
                     /locus_tag="JNX27_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7884097.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4744..6486
                     /gene="iucC"
                     /locus_tag="JNX27_002965"
     CDS             4744..6486
                     /gene="iucC"
                     /locus_tag="JNX27_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7884098.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6483..7799
                     /gene="iucD"
                     /locus_tag="JNX27_002966"
     CDS             6483..7799
                     /gene="iucD"
                     /locus_tag="JNX27_002966"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7884099.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7805..10000
                     /gene="iutA"
                     /locus_tag="JNX27_002967"
     CDS             7805..10000
                     /gene="iutA"
                     /locus_tag="JNX27_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7884100.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10955..14842
                     /gene="sat"
                     /locus_tag="JNX27_002968"
     CDS             10955..14842
                     /gene="sat"
                     /locus_tag="JNX27_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY7884101.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4025 a         3554 c         3708 g         3648 t
ORIGIN      
        1 gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg ctaataaaat
       61 catggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      121 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtcc
      181 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
      241 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      301 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      361 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      421 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      481 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      541 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      601 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      661 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      721 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      781 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      841 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      901 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      961 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
     1021 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
     1081 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
     1141 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1201 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1261 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1321 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1381 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1441 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1501 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1561 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1621 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1681 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1741 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1801 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1861 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1921 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1981 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     2041 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     2101 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     2161 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2221 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2281 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2341 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2401 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2461 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2521 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2581 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2641 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2701 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2761 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2821 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2881 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2941 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     3001 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     3061 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     3121 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3181 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3241 cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag tgctaaagcc
     3301 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3361 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3421 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3481 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3541 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3601 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3661 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3721 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3781 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3841 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3901 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3961 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     4021 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     4081 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     4141 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4201 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4261 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4321 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4381 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4441 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4501 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4561 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4621 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4681 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4741 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4801 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4861 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4921 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4981 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     5041 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     5101 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     5161 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5221 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5281 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5341 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5401 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5461 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5521 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5581 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5641 cgctggctgc aacaggtttt tgcgaccgat gccaccctgg tgcaaagcgg cgcagtgatc
     5701 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5761 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5821 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5881 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5941 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     6001 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     6061 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     6121 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6181 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6241 ggcgtacctg aaaggcgatt ttatcaactg ctggcagaag tgttgagtga ttacatgaac
     6301 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6361 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6421 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6481 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6541 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6601 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6661 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6721 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6781 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6841 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6901 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6961 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     7021 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     7081 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     7141 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7201 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7261 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7321 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7381 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7441 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7501 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7561 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7621 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7681 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7741 agtggcgcag cggcagccct gcttctttaa ctcactatac agcaaatatt caggaataac
     7801 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     7861 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7921 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7981 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     8041 tgtcagcagc caaagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     8101 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     8161 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     8221 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     8281 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8341 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8401 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8461 cggcctgcag cactctaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8521 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     8581 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8641 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8701 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8761 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8821 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8881 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8941 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     9001 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     9061 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     9121 agatgatttc atcgactaca cgcagcaaca gaagattgct gccgggaagg cgatatctgc
     9181 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     9241 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9301 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9361 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9421 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     9481 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9541 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9601 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9661 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     9721 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9781 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     9841 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9901 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9961 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
    10021 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10081 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
    10141 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10201 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
    10261 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10321 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10381 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10441 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
    10501 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10561 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
    10621 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10681 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10741 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa
    11701 tcttgaaata ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa
    11761 atgggtatta gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg
    11821 gataacaaaa tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat
    11881 aaatctgaat ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa
    11941 tgcagatacc actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa
    12001 tgggggaaat gttctgttta aggataattt ggattttggt agcggtggta ttatctttga
    12061 cgaaggccat gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga
    12121 tatcggaaaa gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca
    12181 caaaataggc cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg
    12241 tgaaggaaat gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg
    12301 aaatggtaag gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat
    12361 attttttact aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag
    12421 aattgccgcc actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt
    12481 tctggcaatc aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa
    12541 actaacgcac aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg
    12601 tagtgtcaac acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca
    12661 tgcaacagag catgcaatat tcagaagcag aacgagtcat tgctccctgg tatttctttg
    12721 tggaacggac tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa
    12781 ttctgattac aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg
    12841 ggtgtttaaa tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc
    12901 caatgttgaa ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc
    12961 ttacattgat aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac
    13021 tatcagtact aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa
    13081 cagccaaata gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa
    13141 taatactcct ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac
    13201 aacaaaaggt attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag
    13261 taaaactgta acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa
    13321 tgcaaatttc attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc
    13381 agcaaccatt actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca
    13441 ggcatgggca gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc
    13501 ccccaaagct acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat
    13561 taatcgtcta gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac
    13621 aacccttaca gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct
    13681 ggcccaagca gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca acctcctgtt
    13741 agtcgacttc attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc
    13801 accaaaagga actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga
    13861 tgtaacaccg gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg
    13921 ctataaatct gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg
    13981 ctataaagcc ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga
    14041 cattaacggt gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg
    14101 tggattcagt gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga
    14161 tggccttgac ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga
    14221 tgccttcagt ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga
    14281 gtccggagca tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc
    14341 aactttcgcc ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga
    14401 agtcggttac cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt
    14461 ttacggtgct gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat
    14521 gaaggataag gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt
    14581 ctccggtaag gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt
    14641 tgccaacggt gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa
    14701 agacggtcgt atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt
    14761 cggtcttgag tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc
    14821 caacttccgt tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg
    14881 gagagaatat gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DADLXX010000101        14927 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLXX010000101 DADLXX010000000
VERSION     DADLXX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.102"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            83..364
                     /locus_tag="JNX27_002969"
     CDS             83..364
                     /locus_tag="JNX27_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884102.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNX27_002970"
     CDS             complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNX27_002970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7884103.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNX27_002971"
     CDS             complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNX27_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7884104.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNX27_002972"
     CDS             complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNX27_002972"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7884105.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNX27_002973"
     CDS             complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNX27_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7884106.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNX27_002974"
     CDS             complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNX27_002974"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7884107.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNX27_002975"
     CDS             complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNX27_002975"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7884108.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNX27_002976"
     CDS             complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNX27_002976"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7884109.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7968..9218
                     /gene="icd"
                     /locus_tag="JNX27_002977"
     CDS             7968..9218
                     /gene="icd"
                     /locus_tag="JNX27_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7884110.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="JNX27_002978"
     CDS             complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="JNX27_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7884111.1"
                     /translation="MKWAVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(10177..10287)
                     /locus_tag="JNX27_002979"
     CDS             complement(10177..10287)
                     /locus_tag="JNX27_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884112.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10340..10744)
                     /locus_tag="JNX27_002980"
     CDS             complement(10340..10744)
                     /locus_tag="JNX27_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY7884113.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10965..11696)
                     /locus_tag="JNX27_002981"
     CDS             complement(10965..11696)
                     /locus_tag="JNX27_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7884114.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11901..13112)
                     /gene="bluF"
                     /locus_tag="JNX27_002982"
     CDS             complement(11901..13112)
                     /gene="bluF"
                     /locus_tag="JNX27_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY7884115.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13426..13662
                     /locus_tag="JNX27_002983"
     CDS             13426..13662
                     /locus_tag="JNX27_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY7884116.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13705..13977
                     /locus_tag="JNX27_002984"
     CDS             13705..13977
                     /locus_tag="JNX27_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY7884117.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKLAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14006..14272
                     /gene="ariR"
                     /locus_tag="JNX27_002985"
     CDS             14006..14272
                     /gene="ariR"
                     /locus_tag="JNX27_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAY7884118.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14385..14633
                     /locus_tag="JNX27_002986"
     CDS             14385..14633
                     /locus_tag="JNX27_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884119.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4004 a         3402 c         3476 g         4045 t
ORIGIN      
        1 catgaggatt ttgcttttga tataagcgac agccaccgcc agaaactgga agaatttagc
       61 ggggtgtaat gaggtcagca gcatgacaga agccgaaata acgggattaa tccgccgcgt
      121 tgccggaatc agccagcagg ctgacgaaca ggccacgcag ccggacagcg tgacagccga
      181 aaactatgcg cgtgttgttg ctgaggtgat gcgccgtgat ggtatagaac ttaatggcgt
      241 ggatatgcgc aacatacgaa ccagagttct tgagttgctg gcctaccgtc gccgcgtgga
      301 gatgtatcgg gagaaggaga aaatcacgta ccactggaag aagccggagc ggttacggcg
      361 gtaactcact gagattaaag caaagcgcaa aattgcgctg gctacatccc tcacatctga
      421 ggactgttaa ccagcaggcc ggaatatatt cagaaactac gaaattttcg cagtttaatt
      481 actcgtcagc ttaatcgcta acccgctctg atattttcgg aaacctcaat ttgaggaagt
      541 cggcgaggta actgtttgat attcccgata gcgcaaaaat gcgcggtcgg gcagaatgac
      601 caccacctca atcgtgacgg agagtgacaa ccagtgacac accgtgacaa aaagacggtt
      661 taaccgttca ttaaaaatcg acgtgatttt gtgctttccg gatgacgtgg ccaacgtcat
      721 tttttagcga aaaattctga ttaaggtcac tggctgaacg gtcataatga ccacgcagac
      781 catcgaggca accatagcga tcatcgttac ggttggagct tttccgccat tggtggaaaa
      841 ggtgcacatc tggatcatca ttacggtgat caagggtatg taaaacccac cagcctgatt
      901 aaaggttaac cggaaaaaaa atccggtagc cccgttatgg ggatccccat atcgacatta
      961 acgcccctca tgaattggtg cgcttccccc tggaaagatt acccgcctgt atatttcttg
     1021 tgtctatttg ttccacgttg tttcatacag tgcaccgaac ggtgtagtta atggtgtagt
     1081 caatttgcga tttttggcac tttttcaatg aaaattgttt aatcgaatca atgacttgat
     1141 gtagtggtaa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     1201 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     1261 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     1321 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     1381 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     1441 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     1501 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     1561 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     1621 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     1681 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     1741 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     1801 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     1861 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     1921 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     1981 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     2041 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     2101 gttaattttg gcatccgcga tgttgccggg tagacgttta ccgccaccga gtgggtcatc
     2161 tctgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     2221 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     2281 agccagtcag gctggatcat tttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     2341 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     2401 aactcgacat gcaatttatt gttttcccac tgcgcaaggg tatagaacag attgctctcg
     2461 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     2521 attccgtagg caagcgaaag caccagtact accgctgccg ttgccaacag aaaacgtacc
     2581 cgcagcgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaggt
     2641 agccctggcc gcgaacggtg gtaatgactt cttgcggata ttgtgcctga atttttttgc
     2701 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     2761 gttgtagcat taacgaatct ttgctgacca ctttgccatt attgcgtatc aacgtttcca
     2821 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     2881 gagagagatc aacctgaaac ggggggagcg aaatgacctg tgaagccaga ccgctattac
     2941 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     3001 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     3061 ataccagaat cggcagtgaa acatcgttgc tacgccagcg gcgaatcagt gacagaccgt
     3121 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     3181 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     3241 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     3301 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     3361 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata atcttaataa
     3421 gcaggccgga cagcatcgcc atccggcact gatttgaggc ttatttcagc tcatcaacca
     3481 tcgtgatagc tcgaccaata tagttagccg gcgtcatcgc tttcaggcgg gctttctctt
     3541 cttctggcaa cgccagacca tcgataaact gcttcatgcc ttcggcgtca acgcgcttac
     3601 cgcgagtaag ctctttcagc ttctcgtacg gtttttcgat gccatagcga cgcataactg
     3661 tctggattgg ttctgccagc acttcccagt tgtgatccag ctcatccagc agatggtcac
     3721 ggttcacttc cagtttgctc acgcctttca gggtggattg atacgcaatc agcgcataac
     3781 cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc tgccagcggg
     3841 aaaccggcag tttgcttgcc agatgctgca ataccgcgtt ggaaaggccc agattccctt
     3901 cggagttttc gaagtcgatc gggttaactt tatgcggcat ggtggaagaa ccaatctcac
     3961 cagcaatggt tttctgtttg aagtggttaa gggcgatata accccagacg tcacggtcaa
     4021 agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa cagttcggca atgtagtcgt
     4081 gcggttcaat ctgggtagtg tacgggttcc attgaatacc cagcgaggtg acgaactctt
     4141 cgctgaactg atgccagtca acttccgggt aagcggcgat gtgggcgtta tagttaccga
     4201 ccgcgccgtt gattttgccg aggatctcca cctggttaag ctggcggtgc tggcgctcca
     4261 tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct ggctgaccgt
     4321 gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta atgccatcaa
     4381 tcagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc attaatgcgt
     4441 gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca gaaaccgcgt
     4501 gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct ttaacgtcgt
     4561 ggttagtggt acgctcgatg gttttgatgc gtgcggcatc ttcttcgctg aaattggcga
     4621 caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc ttgatcgctg
     4681 cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat ttcagcaaac
     4741 catattcgct gaaaatcccg cgcagcgcgc tgagtttatc gccgtagcgt ccatcgacag
     4801 gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt aaatgagcaa
     4861 gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgt agacgtccgc
     4921 cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt gcgcgaactt
     4981 tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga atgcgcgggc
     5041 caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc gtttcggact
     5101 gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc agagtgtcga
     5161 gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt gtgtagcggg
     5221 ttaattcggc gtttaagccc tggcaactgc tggcattgag cacgccgagc agggtttcca
     5281 gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag ctggggttca
     5341 tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc ccctggtgag
     5401 cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg gtgatgtcat
     5461 agtaattctt tgccacgttc actgcttcct tgttttaagt aaagataata atcagaccgg
     5521 cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt tatagaagac
     5581 ggcagactgg cccggcgtca cggcggcaac cggctcatcg aaaatcactt taatgcgatc
     5641 gtcgtccagc gccttgacgg tgcacggaat gtcggtctgg cgatagcggg tttttaccgt
     5701 gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcagctgct gggcaatcaa
     5761 cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa tgttgttttc
     5821 gacgtctttg tccaccacat accacggttc ttcggtgcct tctttggtgc caccgatacc
     5881 cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac caatttcatc
     5941 gccatcgacg gtaatgattt tgcccggttg cgccgggaga taacggccca ggaactcgcg
     6001 gaatttacgt tcgccaatga agcaaatgcc ggtagagtct tttttcttcg cggtgaccag
     6061 accaagatct tcagcaatct tacgcacctg cggtttttcc agttcgccga ccgggaacag
     6121 gctttgcgca atctgctcat ggctgagcgt ataaaggaag tagctctggt ctttattgct
     6181 gtccagacca cgcagcaggc ggctcttgcc atcgacatcg gcccgacgta cgtaatgacc
     6241 ggtagcgata taatcggcac ctaaatcttc ggcggcaaat tcgaggaagg ctttaaattt
     6301 gatctccttg ttgcacagaa tatccggatt cggcgtgcga ccggctttat attcggcaag
     6361 gaacagttcg aagacgttgt cccagtactc ggcagcaaag ttaacggtgt gcagttcaat
     6421 gccgagcttg tcgcagaccg cctgggcatc agccagatcc gctgccgctg tgcaatattc
     6481 ctcaccgtcg tcttcttccc agttcttcat aaacaggcct tcgacctgat atccctgttg
     6541 ttgcaacagc caagcagaaa cggaggaatc gacaccgccg gacatgccga cgattacttt
     6601 ttttgcggtt tcagacattg gatcactcac gacattgaac ttcaaggcgg cgtattctat
     6661 cacgcagcct tttagatgac accctttgta aaaggccagt taaaatcgcc aatcatctcc
     6721 agcggataac gttgcccgct ttgataacaa cgaatacttt ccgccaccag cggcgaacga
     6781 agatttgacg cctgtaaaat ttcttcggcg ctgacccaac ggcagcagtc gatatcgctg
     6841 tcatgaggct gggtcgggca tatttgctca agctcaatgg caaagaggaa acgcaaaaac
     6901 ggcgttttat ctggcgcaat ccactgatgc atacgaataa agtgttgcgg ctgcgcgctg
     6961 atgccggttt cttcccacag ttcacgggcg gcggcttcca ctaaggtttc atcggcttcc
     7021 agatgcccgg caggttggtt ccataacgct ttaccattaa tcgtctcttc aacgactaaa
     7081 aatttgcctt ctgcgtgcac cacgcaagca acggtaacgt gcggtttaaa cattttgcat
     7141 cctcaatctg tcacttctcg ccattcgcca ttggcaagat tatccaaaga gtaatctccc
     7201 atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat gggcggtcat gcggcgcacc
     7261 tggcgattac gtccttcata taaggtgatc ttcagccagc tggtgggaat acttttgcgt
     7321 tcacgaattg gtggattccg cggccataac cacgcgggtt cgtcaaccag ctccgcgcct
     7381 gcgggcaggg tagggccatc atttaaggtt acgccattgc gcaaggcttc aagtgcgtct
     7441 tgtgtgggaa taccttccac ctgcacataa tagatttttc cggtgcgttt acccggctgg
     7501 gttaaacgcg cctgcaacgc gccgttattg gtcagcacca gcaacccttc gctatcgcgg
     7561 tcaaggcgac ctgctgcata aacgccctga accgggatga attcttttaa tgttttgcgt
     7621 ccggcttcat cggtgaactg cggaagaaca tcgtagggtt tattgaagag gatcacacgc
     7681 gtgggctggt tttcatgttt acgcctggta gaacgttgcg agctgaatcg cttaacctgg
     7741 tgatttctaa aagaagtttt ttgcatggta ttttcagaga ttatgaattg ccgcattata
     7801 gcctaataac gcgcatcttt catgacggca aacaataggg tagtattgac aagtcaatta
     7861 caaatcatta acaaaaaatt gctctaaagc atccgtatcg caggacgcaa acgcatatgc
     7921 aacgtggtgg cagacgagca aaccagtagc gctcgaagga gaggtgaatg gaaagtaaag
     7981 tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa cggcaaactc aacgttcctg
     8041 aaaatccgat tatcccttac attgaaggtg atggaatcgg tgtagatgta accccagcca
     8101 tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa aggcgagcgt aaaatctcct
     8161 ggatggaaat ttacaccggt gaaaaatcca cacaggttta tggtcaggat gtctggctgc
     8221 ctgctgaaac ccttgatctg attcgtgaat atcgcgttgc cattaaaggc ccgctgacca
     8281 ctccggttgg tggcggtatt cgttctctca acgttgccct gcgtcaggaa ctggatctct
     8341 acatctgcct gcgtccggta cgttactatc agggcactcc aagcccggtt aaacaccctg
     8401 aactgaccga tatggttatc ttccgtgaaa actcggaaga catttatgcg ggtatcgaat
     8461 ggaaagctga ctctgccgac gccgagaaag tgattaaatt cctgcgtgaa gagatgggcg
     8521 tgaagaaaat tcgcttcccg gaacattgcg gtatcggtat taagccgtgt tctgaagaag
     8581 gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat tgctaacgat cgtgactctg
     8641 tgaccctggt gcacaaaggc aacatcatga agttcaccga aggcgcgttt aaagactggg
     8701 gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat cgacggcggc ccgtggctga
     8761 aagttaaaaa cccgaatacc ggcaaagaga tcgtcataaa agacgtgatt gctgatgcat
     8821 tcctgcaaca gatcctgctg cgtccggctg aatatgatgt tatcgcctgt atgaacctga
     8881 acggtgacta catttctgac gctctggcag cgcaggttgg cggtatcggt atcgcccctg
     8941 gagcaaacat cggtgacgaa tgcgccctgt ttgaagccac ccacggtact gcgccgaaat
     9001 acgccggtca ggacaaagta aaccctggct ctattattct ctccgctgag atgatgttac
     9061 gccatatggg ctggactgaa gccgcagacc tgattgttaa aggtatggaa ggcgcgatca
     9121 acgccaagac cgtaacctat gacttcgagc gtctgatgga aggcgctaaa ctgctgaaat
     9181 gttcagagtt tggtgatgcg atcatcaaga atatgtaatc actacatgtg ttaaatattt
     9241 aaacgggcgt ataacacgcc cgttgtttta tttatgtgga tattattaat agcatatcga
     9301 gcatatttat ataaaaccta ttacttgagc ccatatgggc atatttttat aatacaactc
     9361 ttatgcaaac atttatttgt tatttcgctt tctcctggag gacactcttg actgcttttg
     9421 agtaaactcc ataaattctt gttgaatggt gcaatgttat aaatagtaat aggatattct
     9481 ttttccttaa ggataatacc aagcttaacc ggtgtaaata tccttccagg aggaagaaat
     9541 atagtagatt gataccagat gatcattttc atatcacccc atatggctga aaaagatatg
     9601 ccgcatgtag gttgaattac cgtgtcaatt actgcccact tcatttgtta tgtcttatcc
     9661 cacggtattt aatatagttc atttggatgt tcatttcttt attttgcata tgagtatatt
     9721 accccttcaa aaaataaatt aattaaaacg attgcttata taaaacaaaa tttaaatcaa
     9781 ggaatctcaa tggatgttaa acaaaatgag atttagtgaa aacaataaat tattcacctc
     9841 gttttagatt tgtttagcta taatgttata cattcaaatg aatgaacatc ctgtaattaa
     9901 aacatagcct ttatgctact tggtgccaat ttgctaaaca ttatggttgc ctttttatat
     9961 aacgataata atgaatataa acatgacatg agaataaggt ttcaattttt gagttatata
    10021 ggaaaggttt aacctgttcc tggctaaaat acatataact ggatgatgac taaaccaaaa
    10081 cacatatgcg ttaagtattg aaacgggcgt atggcacacc cgttgttttt ataaatatat
    10141 taaccgttat aaaataacgt ataaaaagtc aagtgatcac atttcaaata tcaatttgat
    10201 agtatgggct tggtgattat ttatgggtag caataaaagg acagtattta tcatccatag
    10261 ggatagtctc tgtactttta ttcccattat gctaatgcct tactgaatta tgaagcattt
    10321 cttaagcatc caactttagc tagattaatg gtttattatt ttctacatct tcaatatata
    10381 aaagcgtatt atcaatggcg tagtaactgc gtttgttatg attaacatca gtaacccaac
    10441 ggaaaacgcc cgcgcctgct agtgttgaac agtattcccg aaatgtagat tttccgcaaa
    10501 tatgaagcaa tgcggcctct tttattttag caggattctt ggtcgtacta acttttaact
    10561 ggttcctggt tcctcttaat aacaaaaccg tgtcatcgtg agtaataatt ctgatgttat
    10621 ccgtagccag ataataaatg taatgtgcaa tacggtgatg ttttaattct gaataaaacc
    10681 aggagaagtt ttgctctttt ctcacttgct caaacatctt ttgaaaaaca acgacctgat
    10741 ccatcaggat aataacctct tgttagttgt gagactgcgt agtgtgcacg atcggtttta
    10801 ccacttcaat ctggtctgtc ctttggctgt gatatgtaca gagtgtgata gagggattac
    10861 ctgaattctc ccggtgagca ttttgcaacg gaccagctcc ggtacaaatg ctgttgtggg
    10921 ttcagattat aacattctgt ctaaggggcg ggataaaggt gaaattaggg ggcatgaaag
    10981 atgactttat aaccttgctc accccaatgt tgtaaaagtt cgttttgcct tctcgttggt
    11041 gccatgcctg tccagacaat caatgtttgc gtcgggaaca gttccgggcg cggcgattca
    11101 atgggatcag caagaacaga aatgtgccat cctgacaatg ataaacgcca tgcttccagc
    11161 cacaatcgtg ctctctcttc aacattccac gccatcagaa tggcttcttt accgggctta
    11221 cggcgcgttt cgaaaagcga ggttgctgcg tattcaatta atgcgccgtc aaacatactg
    11281 ctcataatgc gggaggtgtt gtgatcaagc acgagacgct ggcgaacagg aaggtaaaca
    11341 tgattaatca attgatctac tgggtactct cgacccagtg aaataattct cgcgcgtagt
    11401 ttggcaggat tagccatacg aagaattgac atcatctctt cttgcaggcg gctccagtca
    11461 tcttccgtat cctggctggt ggtttccagt aatgctttaa ctttgcctac agggacgcca
    11521 ttactgatcc aacgcttgat ctcttcgatg cgttgtatgt cttcttcatc aaagagtcgg
    11581 tgtccgcctt cactgcgctg tggttttaac aaaccgtagt ggcgttgcca ggcccggaga
    11641 gtgacaggat taatcccgca acgttcagca acatcaccaa tgctgtaata agccacaatt
    11701 cctccttgcg gtcacaaatc tccgtcgcct gtacacgacc caataatact ttgtacaata
    11761 tacgctaaaa ttgtacaaag tataaataag atttcagcta aattggatga aacattattt
    11821 ttaatgtaga ttaaatttaa acataacgta ttcattttca cgatgattta ctgaaatcat
    11881 gtgaaagaat gtgctgaaaa ttattttttc tctggccacg ctatggaagg gataccattc
    11941 aatttagctt tagcaaacag atctccctga aacatctcaa ttcctgcgga ttcaagccac
    12001 atccactctt ctggtgttgc cacgcccata gcactgactt gaatttcaag tgatgtacag
    12061 cattttatga tcgcttgaat aattgcctgc cgtggcccgc ttttatgaac attggtaatc
    12121 aattcctgac tgattttaat tctgtcaggc tggaagcgtg acaggagtaa caaaccggca
    12181 aaacctgcgc caaaatgatc aattgctaca ctgataccgg cagccttaag cgatttaatc
    12241 gcttctgcaa actcatcaaa ccgagatata acttcacttt cagtaaattc aacgatgatt
    12301 tgttcaggca ccagagcatt ggcctttatt tcatcaagta aaaaagagac tgcgtcaggt
    12361 tcgttaacca gggtcatagg taatagattg attgaaatca ttttatcacc gagctcaagt
    12421 gcgtgtgtca tcgcgaatgc aagcgcctta cttttgagat ccgctgtgta gatttccccg
    12481 tctttacgct gcccaaccgc tatggctgat gggctatctt catttttttg cacaatggct
    12541 tcaaaagcga ttatccgccg cgataaggga tcgacaatag gatgaaaggc aaagtggtcg
    12601 tttatagttg gtgaaagggt acaggaatca agttctttat cagatccgtc agcgataaaa
    12661 agccaggagt cttcggcagg gatctcgaaa taggttgatt gttcggttgc aaggacaaaa
    12721 gtacgaaaaa attgtagcgc tctgtcatca taagttagct gaaattttga tgtgcctttg
    12781 tcgaatacgg cctgtaaaac gtcatctcgc tcgtgcaggc gcaaatcaaa taattccatt
    12841 cccgctttgc caaaacggcg agcaggcgcg taatcgcaca gcagctcaac aatattatag
    12901 tgccgtggat cttggcatat agcccgatat atcattttaa cctgttcttc cggaccttcc
    12961 agaagctgga aaaaatgaga accattaaac agtaagatcc ctgttacgtc agactgcatg
    13021 ttcctgcgat ttgctatcga aaccatttct tcgatttttt tgacaggttc gtcgtcacgt
    13081 atatggctac gataaataag ggtggtaagc attaacaatc cagggtaatg ggtgaggcga
    13141 gagtaagacg gtaacagaca tatcttcttg tgtctttctt ttaataccaa aacataaccg
    13201 tttctttaca ttgataaaaa atggaaaaag ttgaacacga gttggcgaaa aatcttgtat
    13261 agattgtcag ttaaatgatg caatatgttt tatcataaca cattgtttta tatacattag
    13321 tactaattgc aaaaaattaa tttatcattc tgtacacata tttcgtacaa gtttgctatt
    13381 gttacctcac ttcacattga ttaacatttt taacagaggc gtagcatgca tcaaaattca
    13441 gtgacttcag attctgcggg agcaatcact cgttactttg caaaagctaa cttgcctact
    13501 cagcaggaaa ctctcgggga aattgtgact gagattttga aagatggacg taatctgagt
    13561 cgaaagtcgc attgtgccaa acttctttgc cgactggaac aagcgacagg ggaagaggaa
    13621 cagaaacatt ataatgcact aattgggttg ctttttgaat aatgcaacag cctgattatt
    13681 taacaggtta gctatgaagt cgttatgaag acatctgata atgaacgtat aaaatatgaa
    13741 attactggcc aggcggtgct ccagatactg cgcatgaaga taaatttttc attgcagact
    13801 cttattaagc aattactcgt aatgaaattg gctgaggaag atgctttccg acgcgattta
    13861 attgacagca taattcgtga ttttagcaat agtgattcag gagggccaaa ccgaagaaca
    13921 gcgacagctg acaataaaag tatgttcaat ggtaagaaaa taaacagaat acattaaaat
    13981 ttcataagta agatgagagg ttaccatgct tgaagatact acaattcata atgcaataac
    14041 tgataaagcg ttagcaagtt actttcgcag ttcgggtaat ttgttagaag aagagtcagc
    14101 agtgttaggg caggctgtca ccaatttaat gctttcaggc gataatgtta ataataaaaa
    14161 tattatctta agtctgatac actccctgga aacaacaagt gatattctca aagctgatgt
    14221 gattagaaaa acactggaaa tcgtgttgcg atacacagct gatgatatgt aacttctaat
    14281 tattaagtat aagtttatag aaaactcatt catcttattt ttgtctgtcg ctttagacta
    14341 tacacagcat aattttattg ggttaatatt tctacgaggc tgacatgaat aattcaatcc
    14401 cagagagatt tatttttcaa tgtgctttgt ttaaaaatct cgagagggag gtgttcatga
    14461 cgcatgggta tgttgatagt catattattg atcaggcttt acgcttgcgt ttaaaagatg
    14521 aaaccagtgt gattctttct gatctctatc tgcaaatatt gcagtacatt gaaatgcata
    14581 agactacact aacggatatc attattaatg acagggaatc cgtgctctct tagaacttga
    14641 gtaaggtttt agaataaaca tcagctgtat caccatgctg atgcaaagtg agcatttcag
    14701 gcgttatgct ttcttattat gtccgcaata tcaggtgtca agaatggaga gttctcgctc
    14761 tccattcttg acgcctgata tcccgcctaa cttattttgt actgcctaca ttatgctcaa
    14821 taattaatgg gttagatcaa gatggagaaa attttatctt cggcgtggat atttcttatg
    14881 ctctctacca tgcatctctg tagatgccag cgcgagcgaa tgaatgg
//
LOCUS       DADLXX010000102        14694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLXX010000102 DADLXX010000000
VERSION     DADLXX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.103"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            23..589
                     /locus_tag="JNX27_002987"
     CDS             23..589
                     /locus_tag="JNX27_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7884120.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            640..3099
                     /locus_tag="JNX27_002988"
     CDS             640..3099
                     /locus_tag="JNX27_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7884121.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQY
                     CSLHLPEHMEDTANRLILPCH"
     gene            3114..3845
                     /locus_tag="JNX27_002989"
     CDS             3114..3845
                     /locus_tag="JNX27_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7884122.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3842..4903
                     /locus_tag="JNX27_002990"
     CDS             3842..4903
                     /locus_tag="JNX27_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7884123.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIHVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5010..6101
                     /locus_tag="JNX27_002991"
     CDS             5010..6101
                     /locus_tag="JNX27_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7884124.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(6098..6889)
                     /gene="nei"
                     /locus_tag="JNX27_002992"
     CDS             complement(6098..6889)
                     /gene="nei"
                     /locus_tag="JNX27_002992"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7884125.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6925..7659)
                     /gene="pxpA"
                     /locus_tag="JNX27_002993"
     CDS             complement(6925..7659)
                     /gene="pxpA"
                     /locus_tag="JNX27_002993"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7884126.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7649..8581)
                     /gene="pxpC"
                     /locus_tag="JNX27_002994"
     CDS             complement(7649..8581)
                     /gene="pxpC"
                     /locus_tag="JNX27_002994"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY7884127.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8575..9231)
                     /gene="pxpB"
                     /locus_tag="JNX27_002995"
     CDS             complement(8575..9231)
                     /gene="pxpB"
                     /locus_tag="JNX27_002995"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7884128.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(9254..9997)
                     /gene="ybgI"
                     /locus_tag="JNX27_002996"
     CDS             complement(9254..9997)
                     /gene="ybgI"
                     /locus_tag="JNX27_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7884129.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10268..11749
                     /gene="dtpD"
                     /locus_tag="JNX27_002997"
     CDS             10268..11749
                     /gene="dtpD"
                     /locus_tag="JNX27_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7884130.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11899..13317)
                     /gene="phrB"
                     /locus_tag="JNX27_002998"
     CDS             complement(11899..13317)
                     /gene="phrB"
                     /locus_tag="JNX27_002998"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7884131.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13314..13823)
                     /locus_tag="JNX27_002999"
     CDS             complement(13314..13823)
                     /locus_tag="JNX27_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7884132.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            13952..14269
                     /locus_tag="JNX27_003000"
     CDS             13952..14269
                     /locus_tag="JNX27_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY7884133.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGMARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(14324..14644)
                     /locus_tag="JNX27_003001"
     CDS             complement(14324..14644)
                     /locus_tag="JNX27_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884134.1"
                     /translation="MPGYRTWIEISLIDELKPSYPFRLEEYAMIGHSPYANECNKDEA
                     KFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLSTLHVNLEQFINSSEDD
                     EYTL"
BASE COUNT         3594 a         3897 c         3564 g         3639 t
ORIGIN      
        1 ttttctgtta aaggactcag ttatgtttaa aggacaaaaa acattggccg cactggccgt
       61 atctctgctg ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg aaattcactt
      121 taaaggtgaa gttattgaag caccgtgtga aatacatcag gatgatattg ataaagaggt
      181 tgaactcggt caggtgacca ccagccacat taatcagtca catcacagcg atgccgttgc
      241 tgtcgacctg ctcttagtca actgtgatct ggaaaactcc agcaacggtt ccggtggcaa
      301 gatttctaaa gttgcagtca cctttgatag ctcagcgaaa accaccggcg cagatccgat
      361 tctcaacaac accagcacgg gtgaagccac cggcgtcggc gtacgtttaa tgaataaaga
      421 tcaaagcaat atcgttttag gcaccgctac tccagatatc gacctggctc cgacctccag
      481 cgaacaaacg ctgaatttct ttgcctggat ggaacaaatt gatcaggcta cacctgtaac
      541 gccaggcgca gttacggcga atgcaaccta cgtgctggat tataaataag tattactttg
      601 gcggcgctat cgccgccttt cataaaatat ttatcaggta tggacaccgt gaatatttat
      661 cgactctctt ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc ggtcgaattc
      721 aatctgaatg ttctcgataa atcaatgcgc gaccgcattg atatttcatt attaaaggaa
      781 aaaggagtca ttgctcccgg tgagtatttt gttagcgttg cggtaaataa taaccaaatc
      841 agtaacgagc aaaagattaa ctggcacaaa aatgacgata aaaccattcc gtgcatcaat
      901 gatttactgg tcgataaatt tggcttaaaa cctgaagtcc gtcagtcatt accattgata
      961 aatcagtgcg tcgattttag ctcccgacct gaaatgctct tcaatttcga tcaagccaat
     1021 cagcaactga atattagtat tccgcaagcc tggctggcgt ggtactcaga aaactggacc
     1081 ccaccctcca catggaaaga aggtgtcgcc ggtgtcctga tggattacaa cttgtttgcc
     1141 agcagctacc gcccacagga tggcagcagc agcactaatc ttaacgccta tggaacagcc
     1201 ggaatcaaca cgggggcatg gcgcttacgc agtgattatc agttgaatca tactgatagc
     1261 gatgataacc atgaacagtc aggcgaaata tcacgtacct atctttttcg cccgttaccg
     1321 caattaggtt caaaattaac cctcggcgaa acggatttta gttccaatat tttcgacggt
     1381 ttttcttata ccggcgcggc actggcaagt gacgatcgaa tgttgccatg ggaactacgc
     1441 ggctacgccc cacaaattag cggtattgca cagaccaatg ccacggtgac gatcagtcaa
     1501 tcaggccgcg tcatttacca gaaaaaagtc ccaccaggcc catttatcat tgacgacctt
     1561 aatcagtctg ttcagggcac actggatgtc aaagtgacgg aagaagatgg tcgggtgaac
     1621 aatttccagg tttcagcagc atcgacgccc ttcctgactc gtcagggaca ggttcgctat
     1681 aaactggccg cgggtcagcc acggccctcc atgtcacatc aaactgaaaa tgaaaccttt
     1741 tttagcaatg aagtttcctg ggggatgctg tcaaacacct cgctgtacag cggcctgctg
     1801 ctttctggtg atgactacca ttctgccgca atgggtattg ggcaaaatat gctgtggctt
     1861 ggtgcgctgt cgtttgatgt cacgtgggcc agtagccatt ttgatactca gcaggacgag
     1921 cggggcttaa gctaccgttt taattacagc aaacaagtgg atgccactaa cagcacgatt
     1981 tccctcgccg cttatcgttt ctccgatcgt cattttcaca gctacgccaa ctatctggat
     2041 cacaaataca acgacagcga tgcgcaggac gaaaaacaga cgatcagctt atctgtgggc
     2101 caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca aacctggtgg
     2161 aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga tattggtgac
     2221 tggagagata tctctatttc tacgtcattc aatacaaccc attacgaaga taaagatcgc
     2281 gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg ggttggctat
     2341 gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga tacgctggat
     2401 gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtcctga caatggagcc
     2461 caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat ttctggtacc
     2521 tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt caccgcaacc
     2581 caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct gatggtcagc
     2641 accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa ccattttggc
     2701 attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt gaacatgaat
     2761 gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg gattgaaggc
     2821 gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt catcattcgc
     2881 aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga cagcggcatt
     2941 agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc tgaaaatcaa
     3001 aagtttaccg tggtctgggg tgatagccag tattgctcgc tccatcttcc tgaacatatg
     3061 gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg aaaatgacat
     3121 ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac gctgcactac
     3181 aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt ctgcgggtgg
     3241 ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat gaaaaagggg
     3301 agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa ccgaaagcaa
     3361 caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc gatcgcgaaa
     3421 cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac agcgaccatg
     3481 ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg gctgcattac
     3541 gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag ggcaaccaac
     3601 tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga cgaaatgaaa
     3661 aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg gtcaacacaa
     3721 acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat tacggcgcgt
     3781 tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg gaggcgaaga
     3841 aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc cgttgaagtc
     3901 tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa aatcagaagc
     3961 gattcaacct tttgctgtac caggcaatgc caaactcggc gataagatct gggaatctga
     4021 cgatattaaa attcccgtct attgtgacaa caataccaac ggcaactttg aaagcgaaca
     4081 tgtttacgca tgggtaaacc cctacccggg ggtgcaggat cgttattatc aactcggagt
     4141 aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg acactaacca
     4201 gtgtatcgac agtaagaaca ttgacattta tacccctgag cagatcatcg cgatgggatg
     4261 gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgta cctttctcgc
     4321 tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc aaagtacgct
     4381 tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag accctactgc
     4441 ccaaaacctg aaatatcata ttactggtct ggaaaacatt cacgtgctgg attgcagcgt
     4501 caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta atttgctgga
     4561 tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat cacaaaatga
     4621 ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa cgttggttga
     4681 agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg atgaaaacgc
     4741 ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg acatgttggt
     4801 ctacgaaaaa acctatacag ctgaactttc gtccattgca ggtaccccca tcgaggctgg
     4861 gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca tgatgccctt
     4921 tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt ccttgattgc
     4981 tgttattttt tcctagcccg gctttcttta tgctgtatcg atacaccaaa gagaaaataa
     5041 tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta tcacttctcc
     5101 tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg ccgatgatcg
     5161 ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc gcttttctcg
     5221 ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg attctcacca
     5281 cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg ctttccagcg
     5341 ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc ggtgcctggg
     5401 gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac ggcgcagata
     5461 ttcgcctggt ggcgtttatg caatatctgc gagtgctgtt cgtcgcgggg gccgctgttc
     5521 tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa atcgtctggt
     5581 tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt gccggtacgg
     5641 cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg gcgggcgcag
     5701 tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg gcgatggcgt
     5761 atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta ctgcgggcac
     5821 tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt tgtgcaggta
     5881 tggcgtgggg actgacccgg tttatgcata ttgattttat gactgcctac ctcgccacca
     5941 gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc gatatggcgc
     6001 ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacaggg ccagccattg
     6061 cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag cctggacacc
     6121 agtaaaacgg gcgcgaggac aacgtggttt tctcaatgat gccgccacag cgttcgcaca
     6181 gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc ttattctcat
     6241 ccacctgccc ccgcgtagcg taagaaaacc gaggaatctc cagtaacgcg tgtgcgagtg
     6301 catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac ccaacctgcc
     6361 agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga tcgagcagta
     6421 atcctgcaaa ctgacggtta cgaaagcgcg gcgacaataa ccgttccttt accacctccg
     6481 gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga tgcgtggtca
     6541 gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg gttttgtcag
     6601 ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg gtatcaacca
     6661 cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taaaccgttg ggaaaatgag
     6721 ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata agttgtgatt
     6781 gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg cctttgatcg
     6841 ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg gtgatatcct
     6901 tttttcattc tttattaagc ggggttatgc tgcgacaaca atcccctttt tggcaaatgt
     6961 agctcgtaat cggcgggcga acgccagagc gtgctcgccg tcgccatgca ggcagaccgt
     7021 ttgcgccgcg accgttgccc attcgccggt gatacttttg actctgccgt gttgcaccat
     7081 ctccagcgtt tgcgccaacg cctgttcttc gttttcaatc aacgcgcctg gctgacttcg
     7141 cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct cgcgcgttgt
     7201 caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa cgagaaccaa
     7261 tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg tttctttcgc
     7321 cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc cttgcgcacg
     7381 ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg tttccggcgg
     7441 cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt gagcgccaat
     7501 cgcgacacca ttttttattg cttcacgcac gcaagcctgc atggtttgcg catcgcctgc
     7561 atgaaatcca caagcaatat tggcagagga aaccagcgtt aatagctctg cgtcgctggc
     7621 gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc cacgctaatt
     7681 gttcaaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt gaacactgga
     7741 caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca gcctcaatga
     7801 tacaggcaat acgtggataa ccaccggtgg tctgtgcgtc gttcatcaac acaatcggct
     7861 gcccgttatg cggcacctgc accacgcccg gtaacaaacc gtgagataac agttcgcgat
     7921 cggtgatgcg ttttaaaatt tgcccctgta aacgatagcc cattcggtta ctttgcgagc
     7981 taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac tcatgatatt
     8041 ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg ccctgcgctt
     8101 ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc agtaaacggc
     8161 cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt accggcggaa
     8221 catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg cgtttaagcg
     8281 ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggcg ttatcatcca
     8341 accgcgcttc gcaacctgca cccgtcaggg caaaccaccc atcagtttcg aactcaactg
     8401 ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc accagcaggt
     8461 tagcaatgcg cagcgcaggc atatccagtg cgccacagtg gctgataccc gactggcgaa
     8521 aaccgtgacg accgccatcc tgcacagtgg tgtacatgcc cgcacgaata atcttcagca
     8581 tattccctcc ttctgcggta caaatcgcac gctgtctccc ggacgtaata agatgggttc
     8641 gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc agccacccgg
     8701 cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac ctgccggaac
     8761 gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac tcccgagata
     8821 cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg agtgcaattc
     8881 aacaacctgt ttttcgctca acccgcaata cgccgcgacc accgccaaat ccggtccgcc
     8941 tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca gggcctcgct
     9001 ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact caggattacg
     9061 caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg gcatatccac
     9121 cagacgctgc gccagtcgcc agatccgctt ctggctagcc agcgtcaccg gcggttccag
     9181 ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca ctttcgatcc
     9241 ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca agatcggtat
     9301 tttcattcag ccactcgctc aatgcgcgaa taccaccgcg ttcagtggca tggtgacctg
     9361 cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttca gaaacttcgc
     9421 cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt tgcccgccgc
     9481 ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac cataacggct
     9541 tacgtcccag acgcgcttca atccaggagg ctagttccat tcccggcaca ggcatggtca
     9601 gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc agtaacgccg
     9661 ccagctgtgc gttattgccc agctcaggat gtgcgtcaag cggcaaatgc cagccataca
     9721 ggttgatatc attctccagc aacgttttta aacggttacg cttcattccg cgaatgaccg
     9781 gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg cccagacgca
     9841 ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt tgcaccgtct
     9901 ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg ctgttcagtt
     9961 tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc gtattctgat
    10021 agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc ttttgacata
    10081 ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg cttcagctaa
    10141 tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta tcaaagtttt
    10201 aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgccg tcaattgtgg
    10261 aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg ttgcgctgca
    10321 aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct atctcaccaa
    10381 tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact gttcgctggt
    10441 gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca atcgcatggc
    10501 ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg ccagtgagat
    10561 ccatccgtca ttcctctatc tgtccctggc gattatcgtc tgcggctatg gcctgtttaa
    10621 atctaacgta agctgtctgc tcggcgagct gtatgagcca accgatccgc gtcgtgatgg
    10681 cggtttctcg ctgatgtatg cggcgggtaa cgtggggtct attatcgcac ctatcgcctg
    10741 tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg cggttggcat
    10801 gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcactcata cccgcggcgt
    10861 taacaaaaaa gtactgcgtg cgacaaactt tctcctgccg aactggggat ggctgctggt
    10921 tctgctggtg gcaacgcctg cactgattac cgtgctgttc tggaaagagt ggtcggtata
    10981 cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt atcgcaaagc
    11041 agaaaaccag aaacagcgga aggagctggg gctgattgtg acgctcacct tattcagtat
    11101 gttgttctgg gccttcgcac aacagggcgg tagctcgatt agcctttata tcgaccgctt
    11161 cgttaaccgc gatatgtttg gttataccgt tccgaccgcg atgttccagt cgattaatgc
    11221 cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gttaaagaaa gcgtcgcagg
    11281 taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga tgagcgccgg
    11341 attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact cttctctgcc
    11401 actgatggtg ttaggcctcg cggtgatggg ctttgcggaa ctgtttatcg acccggttgc
    11461 catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa ccggcatcta
    11521 catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg atcagacatc
    11581 gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat atattgaagt
    11641 atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga tgatttggct
    11701 gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat aggccagcac
    11761 cgatgcctga tgcgacgctt gacgcgtctt atcaggccta cgtatttcct gcaatttatt
    11821 gaatttgcac aaatttgtag gctggattag gcgttcacgc cgcatccggc atctgggctc
    11881 gattgcctga cgcgtctgtt atttcccctt ccgcgccgcc tcatacgctg ccaacgtttg
    11941 tactctcgcc tctttgtgct cgactatcgg ttgcggataa tccagcttca cacctgcttt
    12001 ctgcgcccac ttccacggct catgcaccgc tttccctggt acatcgcgca gctccggtaa
    12061 ccatcgacga ataaactcgc cctcacggtc aaatttctcg ccctgagtta taggattgaa
    12121 aatacgaaaa tacggcgcag catcagtacc cgtagaagcc gcccactgcc agccaccgtt
    12181 attagctgcc aaatcaccat caatcagctg cgacatgaaa tatcgctcgc cttcgcgcca
    12241 gtcgatcaac aaatctttca ccagaaaact ggctgtaatc atccgtagcc ggttatgcat
    12301 ccagccagtg ctgttaagct gacgcatagc ggcatcaata atcgggtatc ccgttttgcc
    12361 tttctgccag gcctgtaaat gtgcgggatt gctttgccac tgcacacgat ccgtccaggc
    12421 aataaatgga cgatgtttac acaacgaggg gtaatacgtc atcagatgac ggtagaactc
    12481 gcgccagatc agctcactaa gccagacact accggcccca ccgtccagcg cctgcggctg
    12541 ttccgccaac aagcgatgca aacactggcg aggcgataac ccgcccgttg ccaagctggc
    12601 ggacaaacgg ctggtgcctt ccactgccgg aaaatctcgt tgttgctcat actctccagc
    12661 accgttctcg caaaactgcc gtaattgcgc aatcgccgct ttttcttcca ccgggaaatg
    12721 cgcagtatcg aaagactgac gaggataatt cagcgtaatg gatggcgcgg gctttatcga
    12781 tccgctacta cgaacttttg gtgcagcgac gcactccggc atcccttccc gcagccgttt
    12841 cagccaggca ttcttaaaag gcgtaaagac tttgtacatc tcgtgattgc cggtcatcac
    12901 cgcccccggc ggcaggatca cgctgtcatc aaatccttca cacaccacgt tacgcagcgc
    12961 tctttcaacg tccacatccc gcgctcgctc attcacctca tactgatagt tataaaaaag
    13021 atgagtaacg ctgttttccg cgcacacctg tttaactatt tcgacactgg cgacaaagtc
    13081 atccacttca cggaacaata aaggaatacc tttttccgcc agcgcgattt gtagcccatt
    13141 cagttgagca ttgataagtt cagcctgacg cggcgacatg ttatgcgtct cccactggcg
    13201 tggtgtagcg atatacagcg ccagcacgcg tgcagacgaa ttgcggcagg cggcagccag
    13261 tgcgagatta tcgtgcagac gtaaatcctg gcgaaaccag gccagatggg tagtcataaa
    13321 actcctgaat ggcgcaagtt aatcacacgc ggccaaagtt cgaaataacg ctgccctgaa
    13381 agccaggcgt caggataaag cgccatatag tgtttgatac gcatcagcgg cgcaagaagt
    13441 ggttgctcgc cacggcgata actgtcgata agcgcagcca gctgctggcg ttctgtggaa
    13501 tcaatatgcg ggcgaaaata accctgaacg tgcatcaaaa cgttggtgtg atcgcgcggg
    13561 ttggcgggat gagaaagcaa aacaattacg cgttggcggt actggttgta aaaatcatcc
    13621 agattttgcc actgatgaat atcagccaca aacgggccaa gtttgcggta ctcgggctga
    13681 gaatgggcca gaaagacgag tttatagcgg ctgtgataat cgagaagcgc cccgcgcgtt
    13741 aagccatgtt ctttcagtcg atgtaactca tgaagggcga agatacggat tagggtgctg
    13801 tcatcaaatc gttgtagatt cattactgct cctgaacggt atacaggagt aagtgtagac
    13861 gttggtaaat gatgtttgtg aagttaatcc gctagcaggg caagattacg aaagcgcgct
    13921 ccccgcaagc aactaatcaa ggtagttaat catggctgaa cacgatatta cgccagaagt
    13981 caccatttcc aaaacacagc gccgctataa agtcggctat gtcagagccc gccaccagga
    14041 ccgcaaaacc ggtatggccc ggtattatag cctgcacctt aagggcgact ggctgaaaga
    14101 ggccggattt gataccggac ggggtgtcac ggtgaagatt tcggaggggg gcctgaccat
    14161 catcgctgac agtgacgagg tgcaggaact gcgggaggaa ctttatcagg ttaaacaggt
    14221 agttaagggg atgcgggatg ggatgtttag tgtgctgaac gaaagttaaa tattaaaaaa
    14281 gctgggaaga tttaaaacct tcctagcttt ttattctgtc ttattataat gtatactcat
    14341 catcttccga tgaatttata aattgctcca agtttacatg aagagtactt aacctagttt
    14401 ttaactcatt aagtgaagcg ataatttcat attttccaac ataatcatat gatggatcgt
    14461 attcattatc tatgtcagaa gctaaaaatg atgatattcg tagtttaaat ttagcttcat
    14521 ctttattaca ttcattggca taaggtgaat gccctatcat agcatattcc tctaatctaa
    14581 aaggataaga tggttttaat tcatcgatca atgaaatttc aatccatgtt ctatatccag
    14641 gcattaatgg actaatttct ttgagcaaaa cctttccact taataatgta ttga
//
LOCUS       DADLXX010000103        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLXX010000103 DADLXX010000000
VERSION     DADLXX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.104"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JNX27_003002"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JNX27_003002"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7884135.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JNX27_003003"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JNX27_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7884136.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHEPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNX27_003004"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNX27_003004"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7884137.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /locus_tag="JNX27_003005"
     CDS             complement(3965..5611)
                     /locus_tag="JNX27_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7884138.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JNX27_003006"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JNX27_003006"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7884139.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIKSDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JNX27_003007"
     CDS             7086..8594
                     /locus_tag="JNX27_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7884140.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNX27_003008"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNX27_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY7884141.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNX27_003009"
                     /pseudo
     CDS             complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNX27_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNX27_003010"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNX27_003010"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY7884142.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /locus_tag="JNX27_003011"
     CDS             complement(13693..14283)
                     /locus_tag="JNX27_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7884143.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14685)
                     /locus_tag="JNX27_003012"
     CDS             complement(14512..>14685)
                     /locus_tag="JNX27_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7884144.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3685 a         3819 c         3496 g         3685 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaacccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattaagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagtgata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctatctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcgg gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gaacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttcaatttc tgggtaatga ctcca
//
LOCUS       DADLXX010000104        14527 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLXX010000104 DADLXX010000000
VERSION     DADLXX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.105"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(337..627)
                     /locus_tag="JNX27_003013"
     CDS             complement(337..627)
                     /locus_tag="JNX27_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884145.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JNX27_003014"
     tRNA            complement(1227..1302)
                     /locus_tag="JNX27_003014"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JNX27_003015"
     CDS             complement(1408..2115)
                     /locus_tag="JNX27_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7884146.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAIPLSAAWTAWFA"
     gene            2513..4648
                     /locus_tag="JNX27_003016"
     CDS             2513..4648
                     /locus_tag="JNX27_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7884147.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADISPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /locus_tag="JNX27_003017"
     CDS             complement(4697..5953)
                     /locus_tag="JNX27_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7884148.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNX27_003018"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNX27_003018"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7884149.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="JNX27_003019"
     CDS             complement(7299..7574)
                     /locus_tag="JNX27_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7884150.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNX27_003020"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNX27_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7884151.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="JNX27_003021"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="JNX27_003021"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7884152.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="JNX27_003022"
     CDS             9534..9860
                     /locus_tag="JNX27_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7884153.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="JNX27_003023"
     CDS             10044..10763
                     /locus_tag="JNX27_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7884154.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="JNX27_003024"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="JNX27_003024"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7884155.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="JNX27_003025"
     CDS             12102..13109
                     /locus_tag="JNX27_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7884156.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14466)
                     /locus_tag="JNX27_003026"
     CDS             complement(13165..14466)
                     /locus_tag="JNX27_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY7884157.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKHRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3807 a         3477 c         3732 g         3511 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggtattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggcgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatatctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcagtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttatcctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
    13861 acggtgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
    14521 cacaagg
//
LOCUS       DADLXX010000105        13995 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLXX010000105 DADLXX010000000
VERSION     DADLXX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.106"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..423
                     /locus_tag="JNX27_003027"
     CDS             <1..423
                     /locus_tag="JNX27_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884158.1"
                     /translation="HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLL
                     KGATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSA
                     VHIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
     gene            595..702
                     /locus_tag="JNX27_003028"
     CDS             595..702
                     /locus_tag="JNX27_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884159.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(755..1509)
                     /locus_tag="JNX27_003029"
                     /pseudo
     CDS             complement(755..1509)
                     /locus_tag="JNX27_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            1795..2724
                     /gene="pfkB"
                     /locus_tag="JNX27_003030"
     CDS             1795..2724
                     /gene="pfkB"
                     /locus_tag="JNX27_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7884160.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2825..3115
                     /gene="ghoS"
                     /locus_tag="JNX27_003031"
     CDS             2825..3115
                     /gene="ghoS"
                     /locus_tag="JNX27_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7884161.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3221..4081
                     /locus_tag="JNX27_003032"
     CDS             3221..4081
                     /locus_tag="JNX27_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7884162.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4122..4658)
                     /locus_tag="JNX27_003033"
     CDS             complement(4122..4658)
                     /locus_tag="JNX27_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884163.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4805..5472
                     /gene="hxpB"
                     /locus_tag="JNX27_003034"
                     /pseudo
     CDS             4805..5472
                     /gene="hxpB"
                     /locus_tag="JNX27_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            5635..6225
                     /locus_tag="JNX27_003035"
     CDS             5635..6225
                     /locus_tag="JNX27_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7884164.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6358..7748
                     /locus_tag="JNX27_003036"
                     /pseudo
     CDS             6358..7748
                     /locus_tag="JNX27_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(7752..8567)
                     /locus_tag="JNX27_003037"
     CDS             complement(7752..8567)
                     /locus_tag="JNX27_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884165.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINEDFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(8844..9107)
                     /gene="cedA"
                     /locus_tag="JNX27_003038"
     CDS             complement(8844..9107)
                     /gene="cedA"
                     /locus_tag="JNX27_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY7884166.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9290..11551
                     /gene="katE"
                     /locus_tag="JNX27_003039"
     CDS             9290..11551
                     /gene="katE"
                     /locus_tag="JNX27_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY7884167.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPRSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGHVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(11598..12356)
                     /gene="chbG"
                     /locus_tag="JNX27_003040"
     CDS             complement(11598..12356)
                     /gene="chbG"
                     /locus_tag="JNX27_003040"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY7884168.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVPDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12369..13721)
                     /locus_tag="JNX27_003041"
     CDS             complement(12369..13721)
                     /locus_tag="JNX27_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7884169.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RYTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(13826..>13995)
                     /locus_tag="JNX27_003042"
     CDS             complement(13826..>13995)
                     /locus_tag="JNX27_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7884170.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
BASE COUNT         3714 a         3453 c         3218 g         3610 t
ORIGIN      
        1 catgatggtg agccaggatt atacgccgca atggaaaata atcacccttt gtgtgtcaca
       61 cggttcctct ctaaaattaa tggcatcgcc tttaaataca agttgagcaa agctaacatc
      121 atggatttat taaaaggagc tacagcacag ggaacccctg cattatacat cgccatgagc
      181 aagggtaatg aagacgtcgt gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa
      241 cattctttta gtcaacatca gttatttaca ctattggccg ctaaaaatca tgacaacatg
      301 tcagctgttc atatagccat tcatcataag cattataaaa ctgtagaaac atattatgct
      361 gctataaatg tcatcagcca aagcctgagt tttagtgctg atgaaataaa gacgtattta
      421 taaccagcta tttacgatat atatcgatac ataattttat ttttttcata aaacgcagaa
      481 acagtgacca tgtgcacaac gccacccgac ggctgtaata acaaaaaatc ccttgatgcc
      541 tgtccctttt gttacactcc gttatcacgc acaagagata tgcaggacac tggtatgccg
      601 actaaacgct ttgataaaaa acactggaag atggtggtgg tgctactggc aatctgtggc
      661 gctatgttgt tgctacgttg ggcagcaatg atttggggct gagcagtgac aaacgaagac
      721 agccagaccg cataacacgg tccggcagat aattttacat gctgtagccc agcgataagg
      781 ttgtccgacg gtcggtatgt tccggtgcag actctggagg ttcagagttc catgtgacgt
      841 tgtaagccac cttcagtcca aaatgttcat taatagcaac atttaatgcg ctttcggagt
      901 tcagcgttgt gtcttccgcg ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag
      961 tcaactgcca tgcataagcc ccggaggcat agcccagcgg ctgggtttca ctggcattgt
     1021 cggtatattt gtcgtaacgc acacctggac cgaattcaaa gcggaaactg tgtaccgggc
     1081 cattcaaaaa ctgacgacca taacccgcgg tcaacacatc gcgctctcga tagccgttat
     1141 aacggtctgt cagccagctt gcctgaccaa ataaatagtc ataatcagtt aaattaaagc
     1201 ggctacgccc gccgcatatt tttctgaaga acgctcatca ttagaagaag tattactggc
     1261 gttcccccac agcgaccagg cagtggtttg tccataccag gtcatggtgg tgtcagccgt
     1321 aagtgaggag cttttcgtat tgcctgattg tgcaagatat cctgcgttca gattaccttc
     1381 gaaaggtttt ttagcgctgg caggatcgtc catgacagta aaaacggaat catcggcagc
     1441 tgcattcagt gacgcaaaca tgccccccgc caacataacg atggcaggaa ctgtcttcaa
     1501 aagcttcatt tatcaagagt ccgtacaaca aaaaagagac catcacggtc ccggaaactt
     1561 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     1621 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     1681 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     1741 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     1801 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     1861 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     1921 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     1981 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2041 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2101 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2161 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2221 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2281 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2341 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga gtcgcgaact cacccagcca
     2401 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     2461 gtttcccttg gtccacaagg agcgctgggt gttgatagtg aaaactgcat tcaggtggtg
     2521 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     2581 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     2641 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     2701 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     2761 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     2821 aactatggcc agtggcgatc ttgtccgtta tgtcataacc gtcatgttgc atgaggatac
     2881 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     2941 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3001 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3061 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3121 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3181 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3241 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3301 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3361 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3421 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     3481 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     3541 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     3601 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     3661 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     3721 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     3781 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     3841 cattttcgac cctgcctgct actggggtga ccgagagtgc gacctggcga tgttaccgct
     3901 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     3961 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4021 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4081 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4141 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4201 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4261 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4321 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4381 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4441 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     4501 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     4561 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     4621 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     4681 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     4741 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     4801 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     4861 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     4921 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     4981 tctttgtacg cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt
     5041 attgcccgtg ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa
     5101 gctgttgcgt tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta
     5161 catatgctgg aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc
     5221 tcggttgaaa aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca
     5281 aaactgggcg ttgacccact gacctgcgtg gcgctggaag actcggtaaa tggcatgatc
     5341 gcttctaaag cagcctgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat
     5401 ccacgttttg tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac
     5461 cttctcggtt aatgaccagg ggcagtgatc acgctgcccc tggttcttta tctgaatttg
     5521 cattcaactg acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg
     5581 tgcactgtat aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg
     5641 gcggaaggtc accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg
     5701 acgcccgtgc tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt
     5761 ttgttaccgg acatcgatca cccaaagtcg tttcttgggc agcgattaaa atggatatca
     5821 aaaccgatcg cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt
     5881 gcgctgctgg caacctttta ccttaaggtt ccggaaggct ggttcattcc ggctgatgcg
     5941 ctacaaggaa tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc
     6001 ggtgttcccc tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa
     6061 aagggcaacc aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg
     6121 ccccattcat tacccgagaa cagcgctgtt cgttggtcat cgcaaatgat caataccttg
     6181 cagatccagt ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac
     6241 ctttctggaa taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt
     6301 gcgccaacac tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg
     6361 aactttccat taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag
     6421 acccgccata aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg
     6481 gtttttggcc ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct
     6541 gtacagtggt ttaacatcgt tggtaacggc aatgttcaac tgctgcaaat gatcgttatg
     6601 ccgttagtct tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta
     6661 ggcaaaatca gttttctgac catcggtacg cttttgttta ccacgctgat tgcggcgctg
     6721 gtcggtgtgc tggtcaccac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     6781 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga
     6841 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     6901 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     6961 tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt cttaaccgct atcgataccc
     7021 tacaaagctg agtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7081 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7141 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7201 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7261 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7321 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7381 gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg
     7441 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     7501 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     7561 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg
     7621 acatgggccg tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc
     7681 agtggctgaa gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac
     7741 accgttaatt cttatgctgg caacggtccg ttttgtatag gggccgttgc tttacttttt
     7801 aaatgttcca tcgcggcgat gcatactgac tcatatttaa acagatcgtc agcgaaaaaa
     7861 atgtccaact tatcaataca ttcctggctc aatgaatgtg cctgtagtga gaagcttaaa
     7921 acctcatacc cgagacgttt tctaagtgaa ctatctggaa tactcataat atcttcaagc
     7981 acaccacttc ccattcccgg aagatttagt ggattaaaaa gaagattgat attaagcatt
     8041 gatattatcg aaggaaactt taggagactc tcattttttt gaatcgcagc aaaaatgctc
     8101 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8161 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8221 atcccaacaa aatcctcatt aatatctctt atccgaaact cactgccata aagattatta
     8281 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8341 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8401 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     8461 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     8521 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     8581 ttattatctt atttaaatct aagccattta caatataagt ttaagagcga cgccacagga
     8641 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     8701 caattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     8761 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     8821 caaaaccagg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     8881 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     8941 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9001 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9061 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9121 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9181 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9241 ttgaccaaaa aagcggccct tccagtaata aattaaggag acgagttcaa tgtcgcaaca
     9301 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9361 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9421 gccaggtgca caacctaccg cccctaggag cctgaaagcc cctgatacac gtaacgaaaa
     9481 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
     9541 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
     9601 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
     9661 acgtattgtt catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt
     9721 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
     9781 taggttctct accgttcagg gtggcgctgg ctctgccgat accgtgcgtg atatccgtgg
     9841 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
     9901 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
     9961 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10021 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10081 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10141 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10201 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10261 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10321 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10381 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10441 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    10501 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    10561 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    10621 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    10681 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    10741 ataccaggag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    10801 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    10861 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    10921 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    10981 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11041 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc acgtggtagc
    11101 gattttactt aatgatgaag tgagatcggc agaccttctt gccattctca aggccctgaa
    11161 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11221 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11281 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11341 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11401 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    11461 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    11521 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    11581 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    11641 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    11701 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    11761 ggatcacccc gatggcttgc gtcatccggc acttgcagga acagtgcctc gctaatctct
    11821 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattagccgg taaatcacca
    11881 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    11941 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12001 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12061 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12121 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12181 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12241 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12301 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12361 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12421 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    12481 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    12541 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    12601 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcttacaggt catttctacc
    12661 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    12721 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    12781 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    12841 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    12901 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    12961 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13021 ccagatgctt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13081 ccatttacca gcacattctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13141 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13201 atattacaca cgccgataaa gcgtttaaat ccggtataac gataaacggc ttcagtaacc
    13261 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13321 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13381 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13441 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    13501 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    13561 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    13621 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    13681 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    13741 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    13801 tatgtgaatt gtcaggtata acgacttact gattaaattc cgtcaatttc ttacgatatc
    13861 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    13921 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgttttttgg
    13981 caaaattaat acgga
//
LOCUS       DADLXX010000106        13988 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLXX010000106 DADLXX010000000
VERSION     DADLXX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.107"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..454
                     /locus_tag="JNX27_003043"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNX27_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            514..621
                     /locus_tag="JNX27_003044"
     CDS             514..621
                     /locus_tag="JNX27_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7884171.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNX27_003045"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNX27_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7884172.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNX27_003046"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNX27_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNX27_003047"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNX27_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNX27_003048"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNX27_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY7884173.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNX27_003049"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNX27_003049"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7884174.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="JNX27_003050"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="JNX27_003050"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7884175.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNX27_003051"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNX27_003051"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7884176.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="JNX27_003052"
     CDS             complement(9943..10530)
                     /locus_tag="JNX27_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7884177.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="JNX27_003053"
     CDS             10700..11278
                     /locus_tag="JNX27_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7884178.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="JNX27_003054"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="JNX27_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNX27_003055"
     CDS             complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNX27_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY7884179.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGMETDINASKTRQQ
                     LNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="JNX27_003056"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="JNX27_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY7884180.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3333 a         3905 c         3527 g         3223 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgcc cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccaaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt aatgtcatca gatccgaaaa
    13981 taacgaga
//
LOCUS       DADLXX010000107        13890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLXX010000107 DADLXX010000000
VERSION     DADLXX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.108"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNX27_003057"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNX27_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY7884181.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNX27_003058"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNX27_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY7884182.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNX27_003059"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNX27_003059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY7884183.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNX27_003060"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNX27_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNX27_003061"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNX27_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY7884184.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNX27_003062"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNX27_003062"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY7884185.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /locus_tag="JNX27_003063"
     CDS             complement(6140..7279)
                     /locus_tag="JNX27_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7884186.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7938..9521
                     /locus_tag="JNX27_003064"
     CDS             7938..9521
                     /locus_tag="JNX27_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7884187.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNX27_003065"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNX27_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7884188.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNX27_003066"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNX27_003066"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7884189.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNX27_003067"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNX27_003067"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7884190.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..>13890
                     /locus_tag="JNX27_003068"
     CDS             13480..>13890
                     /locus_tag="JNX27_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884191.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT         3226 a         3666 c         3509 g         3489 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgcctacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtatgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatca gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg
//
LOCUS       DADLXX010000108        13826 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLXX010000108 DADLXX010000000
VERSION     DADLXX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.109"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            166..1419
                     /gene="kch"
                     /locus_tag="JNX27_003069"
     CDS             166..1419
                     /gene="kch"
                     /locus_tag="JNX27_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7884192.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1474..1647)
                     /locus_tag="JNX27_003070"
     CDS             complement(1474..1647)
                     /locus_tag="JNX27_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7884193.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1790..3250
                     /gene="cls"
                     /locus_tag="JNX27_003071"
     CDS             1790..3250
                     /gene="cls"
                     /locus_tag="JNX27_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7884194.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3285..3614
                     /locus_tag="JNX27_003072"
     CDS             3285..3614
                     /locus_tag="JNX27_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7884195.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNX27_003073"
     CDS             complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNX27_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7884196.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNX27_003074"
     CDS             complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNX27_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7884197.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNX27_003075"
     CDS             complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNX27_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7884198.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNX27_003076"
     CDS             complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNX27_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7884199.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNX27_003077"
     CDS             complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNX27_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7884200.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="JNX27_003078"
     CDS             complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="JNX27_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7884201.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11115..13790
                     /gene="adhE"
                     /locus_tag="JNX27_003079"
                     /gene_synonym="adhC"
     CDS             11115..13790
                     /gene="adhE"
                     /locus_tag="JNX27_003079"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7884202.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3627 a         3426 c         3221 g         3552 t
ORIGIN      
        1 gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt tgattactgc
       61 ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg
      121 tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct
      181 acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg
      241 gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg
      301 cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc
      361 ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca
      421 aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat
      481 ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg
      541 atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt
      601 agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag cgaaggtttt
      661 aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc
      721 gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc
      781 attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc
      841 cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg taaagatcat
      901 tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc
      961 ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa
     1021 cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag
     1081 aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga tgcagataac
     1141 gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc
     1201 agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg
     1261 caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat taataatgat
     1321 atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa
     1381 gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac ggaagagtaa
     1441 acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa
     1501 cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg
     1561 cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg
     1621 ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat
     1681 tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat
     1741 ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt
     1801 ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac
     1861 tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat
     1921 ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg
     1981 caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga
     2041 ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt
     2101 caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat
     2161 gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa
     2221 tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc
     2281 attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag
     2341 tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt
     2401 cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca
     2461 tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga
     2521 tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat
     2581 ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac
     2641 cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag
     2701 tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca
     2761 ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca cgccctactt
     2821 tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt
     2881 cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt
     2941 ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca
     3001 taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat
     3061 gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc
     3121 agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt
     3181 atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc
     3241 gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa
     3301 acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt ctcgaactgg
     3361 ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg
     3421 gcggcgcgga gttattcgat ccggtggaag actggcagga acatgttgat ttcgatttga
     3481 acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca
     3541 acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca
     3601 tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta
     3661 ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag
     3721 aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca
     3781 accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt
     3841 cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg
     3901 tagtggattg tggtagacct catcataggt ccccagttct actgcatggc cgagatacat
     3961 caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa taaaaattaa
     4021 tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga
     4081 cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc
     4141 acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat
     4201 caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg
     4261 gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat
     4321 acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc
     4381 acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc
     4441 ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc
     4501 ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt
     4561 gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt
     4621 aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc
     4681 tccaccggtt taaagcaagc acgcagacgg ccaagcgtaa actcttccag cggcggtgcg
     4741 ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat
     4801 cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga
     4861 cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg
     4921 cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc
     4981 cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc
     5041 agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt
     5101 tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga
     5161 cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt
     5221 tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta
     5281 tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc
     5341 attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct
     5401 ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg tccgttggca
     5461 gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca
     5521 atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg
     5581 ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt
     5641 tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat
     5701 ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca
     5761 ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc
     5821 catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc
     5881 taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca
     5941 caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa
     6001 cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac
     6061 gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc
     6121 cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca
     6181 gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa
     6241 gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc
     6301 cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac
     6361 cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact
     6421 gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg
     6481 caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt
     6541 cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca
     6601 tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac
     6661 aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga
     6721 atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat
     6781 ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca tataggagag
     6841 cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc
     6901 tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt
     6961 aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa
     7021 tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat
     7081 catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc
     7141 catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac
     7201 acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact
     7261 ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc
     7321 atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata
     7381 tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct
     7441 tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat
     7501 cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct
     7561 tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc
     7621 attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg
     7681 tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa
     7741 tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct
     7801 cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg
     7861 cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt
     7921 ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg
     7981 ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag
     8041 aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca
     8101 acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt
     8161 tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca gtatacggtg
     8221 gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc
     8281 ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt
     8341 tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct
     8401 cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac
     8461 cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt
     8521 taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt
     8581 ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc
     8641 atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa
     8701 gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa
     8761 tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg
     8821 cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac
     8881 gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat
     8941 ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc
     9001 caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga
     9061 tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat
     9121 tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca
     9181 gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg
     9241 tgatgttggt cattgttttt tggactccct cattataatt actggtatta accagcatgt
     9301 gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat
     9361 cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat caccttatgt
     9421 cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg
     9481 ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc
     9541 ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgaccgta
     9601 tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag
     9661 attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt
     9721 taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt actaatatca
     9781 tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag
     9841 gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta
     9901 ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga
     9961 gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt
    10021 aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt
    10081 aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa
    10141 tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat
    10201 gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa
    10261 cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg
    10321 cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac
    10381 caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gtagaatcgt
    10441 gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt
    10501 agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat
    10561 ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc
    10621 aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat
    10681 aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac
    10741 ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac
    10801 caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt
    10861 gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt
    10921 gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta aactctttta
    10981 gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcattggtt aagcaaatca
    11041 tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt
    11101 atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg
    11161 taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc
    11221 gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg
    11281 aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata
    11341 tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg
    11401 gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc
    11461 cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac gccattatct
    11521 tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc gttctgcagg
    11581 ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg
    11641 aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc
    11701 cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggta
    11761 acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca tctgtactga
    11821 tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg
    11881 actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg
    11941 gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg aacgcggcta
    12001 tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca
    12061 ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa
    12121 aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag
    12181 cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg
    12241 ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct cgtatcctga
    12301 ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt
    12361 ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac gttggtccga
    12421 aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac
    12481 ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta
    12541 ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg
    12601 ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag
    12661 tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca
    12721 aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagatcatgt
    12781 gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata
    12841 tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca
    12901 ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta
    12961 ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg attgtcgacg
    13021 ccaacctggt tatggacatg ccgaagtccc tttgtgcttt cggtggtctg gacgcagtaa
    13081 ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc
    13141 tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa
    13201 atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga
    13261 acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc
    13321 cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca
    13381 acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg
    13441 ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct gctaagatcg
    13501 agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc
    13561 gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg
    13621 cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac
    13681 agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa
    13741 aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc
    13801 tgtctggcaa tataaacggc cccttc
//
LOCUS       DADLXX010000109        13524 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLXX010000109 DADLXX010000000
VERSION     DADLXX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.110"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..892)
                     /locus_tag="JNX27_003080"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="JNX27_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1114..1914
                     /locus_tag="JNX27_003081"
     CDS             1114..1914
                     /locus_tag="JNX27_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7884203.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1992..2762
                     /locus_tag="JNX27_003082"
     CDS             1992..2762
                     /locus_tag="JNX27_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7884204.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2810..4168)
                     /gene="mltD"
                     /locus_tag="JNX27_003083"
     CDS             complement(2810..4168)
                     /gene="mltD"
                     /locus_tag="JNX27_003083"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7884205.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4240..4995)
                     /gene="gloB"
                     /locus_tag="JNX27_003084"
     CDS             complement(4240..4995)
                     /gene="gloB"
                     /locus_tag="JNX27_003084"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7884206.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5029..5751
                     /locus_tag="JNX27_003085"
     CDS             5029..5751
                     /locus_tag="JNX27_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7884207.1"
                     /translation="MKPARVPQTVVAPDCWGDLPRGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5748..6215)
                     /gene="rnhA"
                     /locus_tag="JNX27_003086"
     CDS             complement(5748..6215)
                     /gene="rnhA"
                     /locus_tag="JNX27_003086"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7884208.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6280..7011
                     /gene="dnaQ"
                     /locus_tag="JNX27_003087"
     CDS             6280..7011
                     /gene="dnaQ"
                     /locus_tag="JNX27_003087"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7884209.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7144..7220
                     /locus_tag="JNX27_003088"
     tRNA            7144..7220
                     /locus_tag="JNX27_003088"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7178..7180,aa:Asp,seq:gtc)
     gene            complement(7351..8397)
                     /locus_tag="JNX27_003089"
     CDS             complement(7351..8397)
                     /locus_tag="JNX27_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY7884210.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8408..9283)
                     /gene="tssG"
                     /locus_tag="JNX27_003090"
     CDS             complement(8408..9283)
                     /gene="tssG"
                     /locus_tag="JNX27_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY7884211.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9400..11283)
                     /gene="tssF"
                     /locus_tag="JNX27_003091"
                     /pseudo
     CDS             complement(9400..11283)
                     /gene="tssF"
                     /locus_tag="JNX27_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(11299..11793)
                     /gene="tssE"
                     /locus_tag="JNX27_003092"
     CDS             complement(11299..11793)
                     /gene="tssE"
                     /locus_tag="JNX27_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY7884212.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11790..12620)
                     /locus_tag="JNX27_003093"
     CDS             complement(11790..12620)
                     /locus_tag="JNX27_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY7884213.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12607..12984)
                     /gene="tagK"
                     /locus_tag="JNX27_003094"
     CDS             complement(12607..12984)
                     /gene="tagK"
                     /locus_tag="JNX27_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HAY7884214.1"
                     /translation="MLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVT
                     GGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTRKEHR
                     IIGIDNHYRINPAQHGEIYHDKQ"
     gene            complement(13221..13391)
                     /locus_tag="JNX27_003095"
     CDS             complement(13221..13391)
                     /locus_tag="JNX27_003095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HAY7884215.1"
                     /translation="MNDDVKIALTLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRL
                     NEGDLIEWGLSS"
BASE COUNT         3094 a         3520 c         3611 g         3299 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaata gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctattttgaa tttatttgtc attaaaccat caggatgcgt gatatgtcat ccgatttaat
      961 gttctcaata atgagcaaat ttctgaccgg tgtaagcact tgcttacata acaatataca
     1021 attgctcgtt gaaagagtga gctaaaatcc ctataacagt agaaccctcc cgagtgcgga
     1081 agggttgacg taatagaggt ttcaaagtca aaagtgcgaa aaaacaccta tgccatgcgc
     1141 tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg ggtcttttgc gagcatcggc
     1201 caggcattac cacctggaga accgttaagt accgaagagc gtattcggat cctggtgtgg
     1261 aacatataca aacagcaacg cgctgaatgg ttgtcggtat taaagaacta cggcaaagat
     1321 gcacatctgg tgttattgca ggaagcgcag acaacgccag agttagtaca gtttgcgacc
     1381 gctaactatc ttgccgccga tcaggtgccc gctttcgtgc tgccacaaca tccttccggt
     1441 gtaatgaccc tttcggcggc gcatccagtg tattgctgcc cgttacgtga acgagaaccc
     1501 attttgcgtc tggcgaagtc ggcactggtg acggtctatc cattacctga cacccgcctg
     1561 ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg gcgtggatgt ctatagtaag
     1621 cagttacttc ctattggtga tcagatagct caccacagcg gcccggtcat tatggcggga
     1681 gatttcaatg cctggagccg tagaaggatg aacgcgttat atcgctttgc gcgggaaatg
     1741 tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc gggcgtttgg ccgcccgctc
     1801 gattttgttt tctaccgtgg tctgaacgtc agcgaagctt ctgtattggt tacgcgcgct
     1861 tccgatcaca atccgctact cgttgaattc agtcccggca agcctgataa ataaggtatg
     1921 tcaggtctgc cacagggcag accaacgttt ggcgctgtcc ttttatactc aaacaacaaa
     1981 aggacagcgc aatgacaaca caatcccacc atgaccacgt agaaaagcag tttagctctc
     2041 aagcctgcga atatctgacc agtaccgtac atgcatccgg acgagattta cagcgcctgg
     2101 cggtgcgtct ggctgattat cctggtgcaa gtgtacttga tatgggctgc ggagcagggc
     2161 atgccagctt tgttgctgcg caaaacgtga gcgcggtggt ggcgtatgac ttatctgccc
     2221 aaatgctgga tgtcgtggca caagctgccg aagcccggca actgaaaaat atcaccaccc
     2281 gccagggata tgccgaaagt ctgccatttg ccgataacgc atttgatatt gttatcagcc
     2341 gttattctgc ccatcactgg catgatgttg gtgcagcact gcgtgaagtg aaccggatat
     2401 tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc tccgggtcat ccagtgcgcg
     2461 acatctggtt acagacggta gaagcattac gcgatacctc tcacgtacga aactacgcca
     2521 gcggtgagtg gttgaggtta atcaatgaag ccaatctgat agttgataat ttaattacag
     2581 ataagttacc gctggaattt tgttcatggg tcgcgagaat gcgtacgcca gaagcgttag
     2641 tagacgctat tcgcatttac caacagagcg catcgacaga ggtgagaacg tattttgcct
     2701 tgcagaatga cggctctttc accagtgata tcatcatggt agaagcacat aaagcggcat
     2761 aaataaaaaa ggcaccgggg gaatcggtac ctttttatta tctggtttgt caggaatctg
     2821 gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc tggttgcaga ttcgcagtgt
     2881 cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg gcgtttagca atgcttgaaa
     2941 gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct gttgtttgcc agtcgctgtg
     3001 cgctactgcc tgcaccaatc gtcaaacttt ggcctggctt cagcttagat ccgcgcagtt
     3061 tgttccactg ctgcaaatct ttggtgctta cgccgagacg tgaagcgatg cttgaaagcg
     3121 tgtcgccaga gcgtacggtg taaacacggc tgttaagcgg cgtattgtcg gcaaccagcg
     3181 tcgactgtac agcagcaatt tcgcctgaag cgagagattc acgcagttga tctgcatgct
     3241 tctttggcac catcacgtac tgcggcccac ttgcgcccag cgtggagcct ttcacgccag
     3301 cgttgaatgt cttcagcttg ctgacggaaa tccccgccat atctgcaacc ttcgccattt
     3361 caaccgggct actcaggtgc acacgcgcca gagcacggct ttcatcggtc gttggcagac
     3421 gtacgccata acgcttgctg tttttgagaa tatcactcaa tgccagcatt ttaggcacgt
     3481 actgcttcgt ttcctgcggc aacggtaacg accagaagtc cgtggatttc ccacgcgctt
     3541 tgttcgtttt aattgccttc atgacccgac cttcgccgct gttataagcc gctacggtca
     3601 gaagccagtc gccgtcaaac attttgttca gacgctgcat catattcagc gcggcagttg
     3661 ttgaagcaac aacatcgcga cgcgcgtcat aattgcgggt ctgtttcaaa ccataattgc
     3721 gccccgtgct cggaatgatc tgccagatgc ctgcggcatt ggcgccagac gttgcgtgag
     3781 gatcaaaagc gctctccact atgggtagta gtaccagttc cataggcatg ttacgttttt
     3841 taacttgccc tgctatccag tacatatacg gctctgcccg taaagttaca tcgtggagat
     3901 agctcttatt gcgtaaatat ttctgtttct gttcgcgaat ccggtcattt tccggaattc
     3961 ccatctttag ctcgtcgcca atgaaagccc acaagtcacc atctggcgcg atagacgtcc
     4021 catcgtccat ccatcgtgcc tgacttgtaa actttgctgc ttccccttga ccagctgcag
     4081 aaaggctctg tgcgtgctgt tgaacgttgc cggtactctg gcaacccacg agcaggacag
     4141 aggcgagtaa tatcgctttt gccttcatgt gtgtgtcaat agttgcttaa aagacgaccg
     4201 atcataacgg cgaacggagc cgatgacaag aaagttttat cagaacctat ctttctttga
     4261 ccttaaccat gcaaaacgct cttcaggttg ttgcaataat gtttcttcat taattacatt
     4321 aattaaatca atatcttccg ttcttaaaaa aacattaatt tgccgctcat ttttcagaat
     4381 tacgggtagt gttatttgat tttttgcccg taactcctta actttacgat aataatcatt
     4441 tatggacaaa tcgtgcggaa aaatactcaa agcaaacttc atatttgata acgtatattc
     4501 atgagcacaa cataccaatg tatcgtcagg taacgcactt agctttttaa gtgattgata
     4561 catttgtaaa gctgtccctt caaacaaccg accacaccca ccagaaaaca gcgtgtcgcc
     4621 gcaaaataga taaggtttac tgaagtaaca gatatgtcct aaagtgtgac ccggtgtggt
     4681 aattacacta aattcatgcc ctaaaacgaa ggcagtttcg ccatctttga ctacctgtgt
     4741 tgttccctta tcttgtgtct cttgtggacc atacaccaca atttgcggaa acttttccac
     4801 cagttctttt acgccgccaa cgtgatcgtg gtgatggtgg gttagaaata tggcctccgg
     4861 ttgccagtta ttggcggtaa tggcgtttaa tactggctct gcgtctccgg gatcgacaat
     4921 caggcagcga cctgcctcat cattcaaaac ccagatgtaa ttgtcatcaa aggcgggaat
     4981 actgttaaga ttcatagatt acctctcagt gtaaaacgga aggttgtgat gaaaccggca
     5041 agagtccctc aaactgtcgt ggctcctgat tgctggggcg atttgcccag gggagagctt
     5101 tatcgcaagg cgctggagcg ccagctcaac ccgtggttca ctaaaatgta tggttttcat
     5161 ctgcttaaga ttggcaattt aagcgcagaa atcaattgcg aagcgtgcgc ggtttctcat
     5221 caagtgaatg tttctgcgca aggaatgccc gtccaggtgc aggcggatcc acttcatctt
     5281 ccttttgccg ataaatccgt tgatatttgt ctactggcac ataccttgcc gtggtgcacc
     5341 gatccgcatc gtttattacg tgaagccgat cgggtactga ttgatgatgg ctggctggtg
     5401 attagtggct tcaaccccat cagtttaatg ggattacgca aacttgtgcc ggtattgcgc
     5461 aaaacctcgc cctataatag ccggatgttt actctgatgc ggcaactgga ctggctctct
     5521 ttgttgaatt ttgaagtgct gcacgccagc cgtttccacg ttctcccgtg gaacaaacac
     5581 ggaggaaaac tattgaatgc gcatattcct gcgcttggtt gcttacaact tattgttgcc
     5641 cggaaacgga ctattccttt aacgctaaat ccgatgaaac agagtaaaaa caagccacga
     5701 attcgccagg cggttggagc cacccggcaa tgtcgtaaac cacaggctta aacttcgact
     5761 tggtagcccg tatcttccag tgtgggattc atcgccgcgg cacgagccag ttcatcacag
     5821 cgttcgtttt ccgggtgtcc ggcatggcct ttaacccatt cccatttgat ttgatgctgc
     5881 cccaatgcag catcaagacg ttgccagaga tcgacatttt ttactggttt tttgtctgct
     5941 gttttccagc cacgtttttt ccagttatgg atccactggg tgataccctg gcggacatac
     6001 tggctgtcgg tactcaaaat gacttcgcaa tgttctttta acgcctccag cgcgacaata
     6061 gcggccatca actccatacg gttgttggtg gtgcgggtgt agcctgcgct aaaggttttc
     6121 tcgcgtccgc gatagcgtaa aatagcgccg taacccccag gtccaggatt acccagacac
     6181 gaaccatcgg tgaaaatttc tacctgttta agcatctctg gtagacttcc tgtaattgaa
     6241 tcgaactgta aaacgacaag tctgacataa atgaccgcta tgagcactgc aattacacgc
     6301 cagatcgttc tcgataccga aaccaccggt atgaaccaga ttggtgcaca ctatgaaggc
     6361 cacaagatca ttgagattgg tgctgttgaa gtagtgaacc gtcgcctgac gggcaataac
     6421 ttccatgttt atctcaaacc cgatcggctg gtggatccgg aagcctttgg cgtacatggt
     6481 attgctgatg aatttttgct cgataagccc acgtttgccg aagtagccga tgagtttatg
     6541 gactatatcc gcggcgcgga gttggtgatc cataacgcag cgttcgatat aggctttatg
     6601 gactacgagt tttcgttgct taagcgcgat attccgaaga ccaatacctt ctgtaaggtc
     6661 accgatagcc ttgcggtggc gaggaaaatg tttcccggta aacgcaacag cctcgatgcg
     6721 ctatgtgccc gctacgaaat agataacagt aaacgaacgc tgcacggggc attactcgat
     6781 gcccagatcc ttgcggaagt ttatctggcg atgaccggtg gtcaaacgtc gatggctttt
     6841 gcgatggaag gagagacaca gcagcaacaa ggtgaagcaa caattcagcg cattgtacgt
     6901 caggcaagta agttacgcgt tgtttttgcg acagatgaag agctggcagc acatgaagcc
     6961 cgtctcgatc tggtgcagaa gaaaggcgga agctgcctct ggcgggcata aatacatgta
     7021 aaaggcgcta aaaatagcga cttgggcgat ttttgcagca aacgattcaa aagatgagaa
     7081 aaatcgttga cgaaggtcga ggcaatccgt aatattcgcc tcgttcccaa cggaacacaa
     7141 cgcggagcgg tagttcagtc ggttagaata cctgcctgtc acgcaggggg gcgcgggttc
     7201 gagtcccgtc cgttccgcca atattcagaa agcccgaatc agtaatgatt cgggctttcg
     7261 tcgttttggt gaagtgagaa tttttcactc cgaagtaaaa cggatatgat ttaaaaaatg
     7321 gccagacatt ataaattctg gccacttctg ttactccagc gcatcccatt tctccagcag
     7381 ttctactgga atttcattta ttacctccga gacgcgtttt ccgaccaggc gttctgcctg
     7441 gcgcagcaaa ggaatggtcg ggctgctcgg ttcgctgtgc tcaaaccagc gacggatgac
     7501 acgcaggcgt tccagcgcgt cgtttcggtc gcggatctgc cggggttctg cgccggtcat
     7561 cggtgatatt tcctccgcga tatgcaccgg tagcgcaatc tgctctgcct gcatctgttc
     7621 agtatgaact atggtggcgt tttccggctg ctcagcctgt ggttgtggca ggatctccgg
     7681 cgtggtgctc tgcactgctc ccggtaacag ggccagcagt tgcgtcagac gggagaagtc
     7741 cggtgcctgg tcattcagcg tttcacgcgc atggcgttgc agccgctcag tggtctctgc
     7801 tgcctggcga aaggcatcca gcggcagtgt gccgcgcgct tccaggtctg ccagttgctg
     7861 gcgtaccgat tctggtgcca gcgcatccgc cgggcgtaat gcagatagcg aacgctccac
     7921 atcccgcacc tgaaggcgca tggcagcatt atttgacagt gtaatgccac gaatatccgc
     7981 cattacacct tcgtggtcca gcaacgccgc cagcgcgtta ctgcgtgcca ccgcagcatc
     8041 ttcggcggtg atatcttcag tcgcgagcag ttgaggatgg atggcgtccg ggtacgtgga
     8101 gcacaacgtt tccagcagcg tgagcgcctc cgacagtccc ctggcaccgg cctgctggat
     8161 acggcagcgc agcaggacaa ccagaatgcg gatatctttg ctgcgcatca gcagacgatg
     8221 ggcgtcccgc tcaagctccg cccagttaat gttttccggc gtactgacaa aatcgccata
     8281 ttgcgcttct tcacgcggtg cggcgcgggt gaacagcagc aggtattccg gatcgtactc
     8341 cagatccggg ccgcaggggc attccgccga aattgggtca gacaggaata tttccattta
     8401 attatcctca gtgcgccagg tagtgttctg gttcgaaaat catgcccgtc accgggttgt
     8461 cgtgcatggc tttacccgcc catgcagtcc aacccagttg gtacggacca ccggtaacag
     8521 ccggaggggc ggcgtggggt tttagctgta gctcaatttc ccagcagtat tcataaccac
     8581 taaaggcgcg gaccagttct gtcaggacgg gcaggttatt gctgccaggc agaaaacgtc
     8641 ggtaatcctc cagcgtcagc gggccgataa ccagccggaa cttatactgc atgtccggca
     8701 cggcctggcc gatgagtgca ccattgccaa taacggaaga gggggcaggc atccccagcc
     8761 gggtgatctc atcgtccgcc acggctatcc agtgcaggac atactcctgt atccggaaag
     8821 gtacaccgaa atagtgcgcc agcgtggtgg ccagaccgtc cggattgcgg gactcacgca
     8881 ccagatgggc cgatgccgcc agacgagcgt gatctggcaa cgggctgtca gcgctctccc
     8941 gcaaatcctg cccgctgagg ctggcgacat aaaaggcaaa gcggtcatgt tgcggtttat
     9001 ccagaccgcc cccagcggac tgggcgctgc gccatgcctg gtaaaactgc gtcagccagc
     9061 ggtgatgaaa aagatcagaa aaacgggtca gcgtcggatc gcgtcggctt tcagtccggt
     9121 tacgcgccag ttcggtgtag tgcagcggca gtggaccgtt cggcccccac agcccgaggc
     9181 tgtacagact gatgtgcagg cgcccttcct gccagcccac actggcgatt tctcgtggcg
     9241 caaaggtcag cgcgggcgtt tgtccgaggc gaaatttttc catccgtggt tgctaggtgt
     9301 tgcccgccgg aacggtacac aactgcacat ccacgcggcg catcaggttc agaaaaccgt
     9361 aatgccaggg gggttcctgc gcctgagtga aatcgttgct catacgcttc ccctctgtcc
     9421 cgccctgaca ggccaggtca taattttgcc gcgctgcatg ctgtgcagcg tcatctgcga
     9481 gaaggtatta atggaaacat ggcgggcgat atagtgttcc agcaccaggc cgaacagata
     9541 cggactgatg ccggaaaaac cttcttcatc caccgtcagt tcacaactga cgccacgccc
     9601 atagaccagt agtccggaac cgggcagacg gcgggtgacc ggggtcgttt tacaaccaat
     9661 caggctgcgc acctggcgcg attgcggact gtcatgagca ggaataaaca ggttcagcag
     9721 atctcgcaac gcctggccgc cggtacggtg atccagatcg gccagcggca gataattaaa
     9781 agataactgg cggatcaacc gccatgccat ctcacgttca gccagcgggg gctgcggcga
     9841 gcgtggcggg cggataagcc ctacgcccgc caccgggatg gctgcatcca cggttagatc
     9901 gtcccggcca ttgcgggcaa taagacaggg cagatcgcgg ttagtgacca tggccgtgat
     9961 ggtgatgtgg cgcagatttt ccgggtacgg cgcttcatac tggtcaacca gcgacaggaa
    10021 cacttccgag cccgtatagg gggtacgggt gccgtaacgg cgggcgtttt ctgataagcg
    10081 gcggggctcc cggcgcaggg aaaaataacg tccgtgattg ccttcatcat tattgcgggt
    10141 gtggtagagc gggcgaaagg ccatcttccg tgtggtgtcg gtttccagcc cttccacttc
    10201 ctggaccgag aacacctcat aatccagcgg atgggttctg tccaccacca gatgctgttc
    10261 cgtcgtgctg tgcgtgacat cgatccgggc ggtgacgcgg ggaaacaggt tgataaccgg
    10321 ggtacagaac aggctgaact gtgccgcatt tgtctggtgg atcagtcagt ccggcggcag
    10381 gcggttcagc aggataacaa tctccgccac gccaccgtca attttctgta atccggcaga
    10441 caggcctgtc ggggtgaaaa aatagaagcg ttccggacag gcaaaatatt catgcagcag
    10501 gttatgaccg tgaaacacgt tccacgccag cggcagcagc ccctgatccg gctccaggcc
    10561 ttcgtacata acgggctgtt gcagattcac atccagcgcg ccgtcaaaat gccccggaat
    10621 gccagccagg gtcgcgatgg cgctggtatg tagaagttct aacaggtgtg aggccgtacg
    10681 ctcctcgccg cagagataaa acggcagcct gtccagtccg gcaagctgac tgaacgttag
    10741 ttcaccaaag gtgcgcaggg taatacgcag cgccccggcg acgtgaatgt tggccggcag
    10801 gtagcggtgc agggcaggca tatccggtgg cgcggcggtc agtcgcgcct cttcgatggc
    10861 gagcggccac agcgttacat cctggctgct gcgaaactgg caggcagtgc tttcgccttc
    10921 cggaatggcg gagaaaaagg ctgtgtcgcg cgggacggta aatcctttcg ccagatcgcc
    10981 ttcttccgta tcgggatgca actgcgccac gctcattgag ggcgtgggtg tgacgtaatt
    11041 ggggctgacc acttccagcg ggcgctgggt gaaacggggg aattcagcgt caatttttag
    11101 ctgcgtgcgg gcggtcagga agctgaaggc ctcgaccatt cgctctgcat acggatcggc
    11161 aatatccgtt ccctgcatgc ccagccgggc ggcgactttg ggatgacggg cggcaaattc
    11221 ggctcccgtt tcccgcagat agctcagttc gcggttgtag tatcccagta gccgtggatc
    11281 catcggtctc tctctgaatt aaaaaaacgt aatgcgactc tgctccatat ccagcgcgct
    11341 acgtacccgg aatgcagtcg gatacggctg cgtgaggatc tcgccgcgaa tttcaaactg
    11401 taatgtgtta tggccactct ggcggcgctc atcctgaagt gggatgattt gcagggtgga
    11461 cgctttcagc cggggttcaa accgggtgat ggttcgcaaa atggccctgc ggatatcgtc
    11521 ccacttgtgc tcatacagaa agctgcccgc cagtggcggc aggccgtagt taagtacgga
    11581 cgctgccgcg tacggataac gtgcagcatc aatatcatct tcatggctga cggtgttgag
    11641 cagaaatgac aaatcccggc ggatgatctc cttaatctga accgggctga cgctgatacc
    11701 gtggtcgcgt ttctggtttg gggcgtcgtc acacaggcgg tcaaacagcg tgggcagcag
    11761 gtgattgcct ggacgctggc gggaagcgct cacgcgtcgc ttccttcagc ggtgttaaag
    11821 gtacaggtgg acaaatccag caggctgata tcgccgtgac cggtgagcca caccctctga
    11881 cccagcgcct gtaccagtgt ctctcccgga ccgtcctgcc agaccgtttc ccggcacaga
    11941 cgcagggagt cggcagcctt ttcagagccg ctgtagcggg gaaacagcca ggcactgtgt
    12001 gtatcgccat tgttcagggt gacgttgacg ggtttccaga tcaaatcgat cagatgcgca
    12061 ggccgcggcg atttcagcga acagatttgt gaaaacggca gccagatata tgctccgccc
    12121 gcgatcagtt cgagtaccgg accggtacgg gagtcgctgt cgctaatcca gtcaaatgcg
    12181 ccgccattcc actgcccgga agtgtctgtt atggcctcaa gcgcgcggct gcggtgagca
    12241 tcagcttcct gcgcttcacc acgggcgtta caggcgaggg tggtcagcag atcctccatc
    12301 caggcgggcg gcgggagaat aacaccgggc cgctgttcgc cctgaaagca ggcgtggcgg
    12361 tatatctcac agcggatcag ctcgccaaat acctgcgctt cgcgagtata atttgcgtcc
    12421 atccgggcgc atagctggat ctgttgcagt gcccgcgacc agtcgcctgc cacgcacaat
    12481 aactgaaaca ggctgtggcg gcgggaggcc tgcgctgggt gcatcctgac ctgttgctct
    12541 gccatctgaa tgccctccac cacggaataa tcccgcagca gcgccgacaa ggtaatcggt
    12601 agctcgttat tgtttgtcat gatatatctc tccatgttgt gccgggttta tccggtaatg
    12661 gttgtcaatg ccaatgatgc gatgctcttt ccgtgtcaga tctggcagta cggtgttatg
    12721 cgctgtttcc ggggcgtatt ccggggaaag taaatgcagg atatcagggg gagtcttttc
    12781 gacaggccag atagtttcat cttcgccagt ggcatccagt acattgagaa tagcgtcaat
    12841 gcttaaccca ccagtgacca tatcctgcaa tgtctcggct tcggtgacca ggtcacgcaa
    12901 tgaatccaga tcctgcgagg agggctcttc gttaaaagga aaaggatcag catggcgatt
    12961 aagatcttgt tcgggagata acaacgcctc cggtattcct gataaagtcg ggtcagggca
    13021 tttccttttt ccgcaccttc tccggtggag gaggctccat gaacaggaat gatatcgaat
    13081 ggattttgtg atgtcagatg ctgctgcctg aaccaatcca gatccagata ttcggttatt
    13141 gcttccgacg tgatggcagt ctgcgatatc gtttttactg tgctcaggtc cggagatgat
    13201 ggattgccat gtacaaggca ctatgaagat aacccccatt ctatcaggtc gccttcattc
    13261 agacgcatac ggtgatgagg ttccacaatc tggtcgttta ccgtacagca atattcggta
    13321 gactgattga taatccacca tgcttcttcg tggcgggtca gagtgagggc aatctttaca
    13381 tcatcattca ccttaaaaac aggaatccag ggagaggtaa cggtgaagat aatttgtgtt
    13441 ccttcagtcg cgtaaattgt gttatcggcg atcccgtgcg cctgtatttt ttggagtccc
    13501 cacgtaatat ttggtttcat tgtg
//
LOCUS       DADLXX010000110        13463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLXX010000110 DADLXX010000000
VERSION     DADLXX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.111"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            49..579
                     /locus_tag="JNX27_003096"
     CDS             49..579
                     /locus_tag="JNX27_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7884216.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JNX27_003097"
                     /pseudo
     CDS             597..1235
                     /locus_tag="JNX27_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JNX27_003098"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JNX27_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7884217.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3188)
                     /gene="ycjG"
                     /locus_tag="JNX27_003099"
     CDS             complement(2229..3188)
                     /gene="ycjG"
                     /locus_tag="JNX27_003099"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY7884218.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3307..3813
                     /gene="tpx"
                     /locus_tag="JNX27_003100"
     CDS             3307..3813
                     /gene="tpx"
                     /locus_tag="JNX27_003100"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7884219.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3857..5398)
                     /gene="tyrR"
                     /locus_tag="JNX27_003101"
     CDS             complement(3857..5398)
                     /gene="tyrR"
                     /locus_tag="JNX27_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7884220.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5546..6607)
                     /locus_tag="JNX27_003102"
     CDS             complement(5546..6607)
                     /locus_tag="JNX27_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7884221.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6604..8001)
                     /locus_tag="JNX27_003103"
     CDS             complement(6604..8001)
                     /locus_tag="JNX27_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7884222.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8156..9154
                     /locus_tag="JNX27_003104"
     CDS             8156..9154
                     /locus_tag="JNX27_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7884223.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9265..10170)
                     /gene="ompG"
                     /locus_tag="JNX27_003105"
     CDS             complement(9265..10170)
                     /gene="ompG"
                     /locus_tag="JNX27_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7884224.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10215..11297)
                     /locus_tag="JNX27_003106"
     CDS             complement(10215..11297)
                     /locus_tag="JNX27_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7884225.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(11311..11970)
                     /gene="pgmB"
                     /locus_tag="JNX27_003107"
     CDS             complement(11311..11970)
                     /gene="pgmB"
                     /locus_tag="JNX27_003107"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY7884226.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11967..13463)
                     /locus_tag="JNX27_003108"
     CDS             complement(11967..13463)
                     /locus_tag="JNX27_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAY7884227.1"
                     /translation="MCAQQSYDQHLAASTENWRQWWQKRRITVNGGDAHDQQALDYAL
                     YHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRW
                     HNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLV
                     ADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYT
                     EHVNNNAFTSYMVYYNVQQALSIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQ
                     DDSFMTKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSSA
                     SCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDD
                     GIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDA
                     QRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE"
BASE COUNT         3335 a         3647 c         3215 g         3266 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc ttccgtcaga ccaccggttt tatcgagctt aatgttaacc
     2461 atctcatagc gccctttcag cgccttcaga ttgctacgag tatgacaact ttcatcagca
     2521 caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc aggaagcggt
     2581 tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc caacccttct
     2641 gcacgccagg attcatttgc atcaacgatc agcgtggcat cgggcacagc ggtgcgaatt
     2701 gccaccatcc gctcactgat aagatggtta tccagcttca ctttcagtaa tttcgcgcct
     2761 gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat gacaaccgtc
     2821 tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag cgattgctgc
     2881 tgttttcgcg ccgcgagatc ccacaatgca caatccagcg cattacgtgc tgcgccggca
     2941 gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcgg cacgacgctc
     3001 ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg cgtgcattcg
     3061 ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc ttcactgcgg
     3121 cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa taccttaacg
     3181 attctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg
     3241 ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta aacaggaaag
     3301 ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc caattccatc
     3361 ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct gtctgacgtc
     3421 accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag tattgatacc
     3481 ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg caactgagat cgacaacacc
     3541 gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa
     3601 ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct gcaagcctac
     3661 ggtgtggcaa ttgctgatgg cctactgaaa ggtctggcag cgcgtgccgt tgtggttatt
     3721 gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac cgagccggat
     3781 tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg tgttgcacca
     3841 tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat tcccgcaact
     3901 tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc gtgctgggat
     3961 aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt tcgtccagcg
     4021 aaccttccat cgcatcttcg cccacggcta ccgttgctgc gtcataatcc ggcaacaaaa
     4081 tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag atagcgttct
     4141 ttaactgccg cacatttccc ggccacgcat aacgtgtaag tacagtattc aggtcagcgg
     4201 ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta
     4261 acggcataat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc aacacgttca
     4321 gacgataata gagatcttca cggaacacgc ctttttgcac cagttcgacc agattcttct
     4381 gcgtagcgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca acccgacgga
     4441 aagtgccatc attaagaaaa cgcagtaatt tcgcctgcat ccgtggtgac atttccccta
     4501 tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaaccctttc ttcccttccg
     4561 gagcatgacc aaacagttca ctctcgaccg catcttctgg tatagacgcg caattcagcg
     4621 ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca aaaagatctt
     4681 tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg
     4741 cctgttcgac aacgtgcttc attttcgggc tgacggcgac aatttggctg aaggcgctga
     4801 cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat cgcaacatca
     4861 ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca ggcgtaatct
     4921 ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct tgcggttcgc
     4981 tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta tggttgcgca
     5041 ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata tccactttgc
     5101 ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg ctcaacgcca
     5161 gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca cctgcaatac
     5221 ggcgtatttc ggccatcaaa ctgctgaaac tctcaaactc cagttcagca aaattgaggt
     5281 agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt agcacgagta
     5341 gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc agacgcatgg
     5401 gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt ggatagcagg
     5461 aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga
     5521 tatccggtca gcacccgata ttgcattatt tttcgctggg cgttttgcct ttttgcagag
     5581 tttctttcac ctgaccgata agctgacgac ggaaatcccc aaggcgaggt ttgtcatcgt
     5641 caatccacgg cagcgggcgg caaagctcca tagctttaat cccgagtcgt gccgtcagaa
     5701 gtccagcacc aattccctga gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca
     5761 tccagtccat ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg atattcagca
     5821 ataccagctt aaacagacgc aaacggctgt aataccccag ttcaatgcca tacagcgtgg
     5881 caatgcgatt aattaaacgc agattgcgcc aggcgataaa cgccatatcg accaacgcca
     5941 gcgggctgac cgcaatcatc aacgttgatt ccgccgccga acggctgatt tcgcgccgcg
     6001 cctgggcatc taaaactggc tggaccaaat gcgcatacag gctgaccact tcacgatcgt
     6061 tctgcgtttc atggattgag gcataccagc gttgcagcgc cggatgcgac tgatcaatac
     6121 ccgcctgctg cgccagtttt tcgcaaaatg cgcgaccttt gcccgtgccg tggctatgca
     6181 ataaatcacg cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc
     6241 actctgttac cacagaacct acgccagcgc cgataatcaa tgccccagcg gcacatccac
     6301 ccagcgctac ccagtcctgg gtttgccagg cattcattgt ccactgtata ccctgcccga
     6361 caacgcttgc gccaaacaga gccagcccgc ccatcaccat tttgcgccac aggctgcgtt
     6421 tcggacgtaa cgctgcgtcc attaccgctt cgacttgccc ctcttcttcc tgcgcttcgt
     6481 cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga
     6541 attttggatt ctgttcgacc tccagcggac cgtcgaaatc aatacgtggt tttaacggtt
     6601 cggtcatcgc aatttatctc ctattaaaaa ttccagcgcg gcatcaagac gaatatgcgg
     6661 cagcggtttg tcgacatcca tcacctgcgg gcgaaacgct tcaaactgga acccttgctt
     6721 gtcccagaac gcctgaccgg gcaaacgcgc aggaacttca ccggggtaaa cggtgagagg
     6781 cgcaccatcg ctgagccggt taccgcgcaa tgccggaatt ttctcgccgt tgacgtcaat
     6841 aatgccgctg gtggtcgtct gcaccgaggc cagccccagg caatccatac taatcctttc
     6901 aaacgccgca ttttgccagg catcctgaat cagttgttgc agtaatgaaa ccatattggc
     6961 gtgctgatcg accgttacat ggtccgcttt cgtcgcggca aacagcagtt tgtcgataac
     7021 cggcgagaat aagcggcgaa acagcgtgcg ctgcccgtag tggaaacttt gcatcagctg
     7081 cgtcagtgcc agacgcatat cattaaatgc ctgtggccca ctgttgagag gttgcaggca
     7141 atccaccagc acaatctggc ggtcaaagcg cagaaaatga ttcttataga accccttcac
     7201 cactttctcg cagtaataat taaaccgctc gcgcagcatt ccggcattgg tgtgcttatc
     7261 ggcctgcgcc agtttggact cgccccaggc atcgacatcc ggccacggga agaattgcag
     7321 cgcgggcgca cctgccatat ctcccggcaa gacaaagcgc ccaggctgaa taaagtgcag
     7381 cccctgctgt ttacagtggt ggagataatc ggtccacacg gcggcaatgt ctgccagccg
     7441 gttttcgtcg gcaggcgcta gcgggtcaag tccttcgcac atcattcgcc atttcgccga
     7501 ccattcgccg cgctgaccat tgagtaagcc cgtcatctgg cgcgaccagc ttaagtaatc
     7561 ctgcgccagc atcggcaggt cgagcaacca ttcgccggga taatccacaa tttccagata
     7621 cagcgtggag gtatccttaa agtggcgcag cagcgaatcg ttcgatttaa aacgtaacgc
     7681 caggcgaatt tcactgacgc cgcgcgtagg cgtcggccag gcgggaggat caccatataa
     7741 ctgcgccagt ccttcatcat aggtgaagcg cggaatgcca aagtcacgct ggggaatgcg
     7801 ttttacgccc agcaagcgct cttcacgcac cgcgcttaac agcggcaaac gtgctccggc
     7861 atgaatattg agcaactgat taaccatcgc ggtgataaac gctgttttgc cgctgcggct
     7921 aagtccggtt acagcgaggc gcagatgtct gtcgacaccc cgattcacca gcgcattgag
     7981 ttcattttta agtcgcttca ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat
     8041 tcgtaaggat atgaatgtgg gggccaagcc attgattatc aactgcccgt gttgtcatcg
     8101 acgggcgaac tccataagat gtgcagcatt cacgttcgga cggagttcct gacctatgtc
     8161 ccctactatt tatgatattg ccagggttgc aggcgtatca aaatccaccg tatcacgcgt
     8221 gctgaataag caaactaata tctccccgga agcgcgcgaa aaagtgttac gggccattga
     8281 agaattacag tatcagccaa acaagctggc ccgcgcgctg acctcttcgg gttttgatgc
     8341 cattatggtg atttctaccc gttcgaccaa aactacggcg ggtaatccgt ttttctctga
     8401 agttttgcat gccatcaccg ccaaagcgga agaagaaggt ttcgacgtga tattacaaac
     8461 gtcgcacaat ccggcagaag atctgcaaaa atgcgaaagc aaaattaaac agaaaatgat
     8521 taaaggcatt attatgctca gttcgccagc ggatgagtca ttttttgccc aactcgataa
     8581 atacgatatt ccggtggtgg taattggcaa agttgaaggt caatatgccc atgtttattc
     8641 tgtcgatacc gataattttg gtgacagcat tgcgttgacc gatgcattaa ttgaaagtgg
     8701 gcatcaaaat attgcctgcc tgcatgctcc gcttgatgtc catgtttcgg ttgatcgcgt
     8761 taatggctat aagaaaagtc tgtccgcgca taatattgca gtgcgtgata aatggattgt
     8821 tgacggcggt tatacccatg aaacagcact gcaagcagca cggcaattat taagtcagtc
     8881 accgttgcct gaggcagtgt ttgccactga cagcctgaaa ttaatgagca tttatcgtgc
     8941 ggcggcagag aaaaatattg ctattccgca gcagttagcg gtggtgggtt atagtaatga
     9001 aacgctgtcg tttattttaa cgcctgcacc aggcggcatc gatgttccga cgcaggagtt
     9061 aggtcaacaa agctgcgagt tattattccg cttaatttcg ggaaaaccgt caccacagaa
     9121 tattaccgtt gccacgcata tgacgctgaa ataaatacca gctaccttgc ctgaatgccg
     9181 gatgcgatgc tggcgcatct tatccggcct acatttatag atcggataaa gtactcgcgc
     9241 cgcatccggc aatttagccc attatcagaa cgagtaattt acgccgacac ctgcataatg
     9301 gaatttatcg ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac
     9361 ggaaagtccg ttctggaaat cataaccgta aaataaaccc acacggttaa aatcatggcc
     9421 ttcacgttca atatcatcct gccagtccca gttactccag cgatccagcc caatgcgcgt
     9481 atacggcgtc accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa tttcttgtgt
     9541 ggaaaactca ccgttattgc ggctgtcgtc catattgaag ccgcgctcga gataatagtt
     9601 cactcgcaaa gcaaccgttt cgttgaaggt atattgcaga cctgtttccg tttcgacacg
     9661 ggtatcagcg taaccggtcg tgttcaaatc gttggcaaat ttatacatcg acaaccaacc
     9721 gttgaaacgt aaatcatcag tcagtttcac atcccagtct ggcgcgattt tccagcgctg
     9781 catattcgcc gtgtcttttc ccggttcatc aacgtagtga taaccataat tacggaaacc
     9841 gccggtcagg ccgaaactga aatcatcgct ttcgaggaac tgataatgca cctccagctc
     9901 cgggcgatca aaccacgttc cacgtttacc cgcgctataa tctaccggcc cttcctgata
     9961 ataggccaga gcaattctcc acggcccgtt ggcggcatta aaatagactg aaggctccgc
    10021 caacccatcc atatcttcgc cataaccctc gacgttttct atttcgtaca tcgcaccgat
    10081 attaaagtgc cagtcgttct tttcctcggc ctgtgcgcag gccattcccg cacacatcac
    10141 cagtgcggta cagggtaata actttttcat tattgttcct tgtattatcc taggggaatg
    10201 ccttccccct gtatttagcg aattgctatt tccgtttctg catcaaagaa atgacatttc
    10261 gtcatatcaa aatgaatagt gatattttct cctgcatgat aatcatttaa cgcaccagca
    10321 cggaccacta actcgtgccc cccaaccgtg gtgtagagca taaattccgc accggttaat
    10381 tctgccacgc taattttggc ggaaatgtta ttttcctctt gcgcgtccgg atgaatatct
    10441 tccgggcgta ttcccatcac gatgggctta tgcaaacttt cctgtgtttt cagaaccgct
    10501 aatttctctt cgagaatggt taatttaagc gtttccgtaa cgaatttatc gccatcgatc
    10561 gtgccgcgaa taaaattcat tgctggtgat ccaataaatc cggcaacaaa catattcgcg
    10621 ggttggttat aaacggtttt cggcgcacct acttgctgaa caatcccgtc tttcataatc
    10681 acaatccgcg tcgccatggt catcgcttcg gtctgatcgt gggtcacgta gatcatggtg
    10741 gtgttcagtt tctgatgcag cttgctgatc tctgcacgca tttgtacgcg cagcttggca
    10801 tcaaggttag agagcggttc atccattaaa aacacgcccg cttcgcgcac aatcgcccgc
    10861 ccgagcgcca cacgctggcg ttgcccgccg gaaagcgcgc ccggcttgcg tttcaggtac
    10921 tcacgcaggc cgagaatttg cgccgcccag ttcacccgct catcaatcac ctctttggcg
    10981 attttttgca tcttcaggcc aaacgccatg ttgtcgtaaa ccgtcatatg cggatacaac
    11041 gcgtagttct ggaacaccat cgcgatattg cgtgctttgg ctggaacgtc attcattcgt
    11101 ttgccgtcga tcaacagatc gccgccgctg atctcctcaa gcccggcaat catgcgcagg
    11161 gtggtcgatt taccgcagcc cgacgggccg acaaatacga tgaactcttt atcggcaatt
    11221 tccaggttga agtccttcac cacatgcacc tggttatcgt agattttttg aatatgttgt
    11281 aacgaaagct gagccatgtt gattcctttg ctatacgttt tgccagaagg ccgataaccg
    11341 cgaccaggtc agggagtccg ttgagggcaa cagtaattgc gccccggtta agcccgcacc
    11401 gatccccacc gagcgcatac cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc
    11461 aatgccgata catgcctgcg gcggcacgcc cagcccggca caggcagcga gaaagatttc
    11521 cggatccggt ttcgagtttt taagttggga agcatccgcg cagaaggtga aaaactcgcg
    11581 cagctccagc gccgctaaaa tcgtcggcgc attcagggag acggaagcca gccctaccgg
    11641 aatctgctgt gcacggagct ctgccagcaa attgcgaata ccgggcagca cagcattaac
    11701 cgtcagctcg cgcagtgagt ggacgtaaag cagatttttg cgatacgcca gttgcgcccg
    11761 ctcctgcggg ttaaagtcgc cctctttgcc cccgtgttgc agaatgcgcc gcagagactc
    11821 atcgcggctg atccctttta gagattcgtt aaactgcgca tcaatactga tgccaatttc
    11881 agcggcaatc tgctgccacg cctggaaatg cagatgcgcg gtatcggtga ttacaccatc
    11941 cagatcgaaa attacccctt gcagtttcat tcatcctcct gatgcgtggt agcggtccca
    12001 ttgaagggca aaataaaatc acccaaacag aaaacagatt cttcagccac ggatataagc
    12061 tgaccgttca aacgcagtga aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtcg
    12121 agagtgacct gtaattcgca gccctgccag aacagaggga aagacaactg ttgccactgc
    12181 tcaggtaacg ccggatttag atgcaattca ccgtcacgca cgctcacccc ggcaaaaccc
    12241 tgaatcgccc ccagccagat agcgccagtt gcggcagcat ggataccatc atcacaacta
    12301 tgcggatcag caccaagatc gatttcagtc ccctcgcgcc agaactgata actttgggtc
    12361 agcaggccac agcgtgcagc aacaatgccg tggattgctt tacttaatga cgagtcgtga
    12421 atagtgcgcg gttcataaaa ttgcagattg gcaagacacg atgccgatga gaactgctct
    12481 ggcagcatgt aattgagcat caccacatca gcttgtttga ggatctgcat ctcgttcact
    12541 tctgcgcgtg aataatccag cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc
    12601 agattaatcg ccggcttagt cataaacgaa tcatcctgcg gcaaaacacc gtcgggctga
    12661 atttctggca gccgcagttc tttaaggaac atttcggcac gatgaataaa cgcgtcgtca
    12721 ctacagccaa actggcgggc aatactcagc gcctgctgga cgttgtaata caccatatag
    12781 ctggtgaagg cgttattatt gacatgttcg gtatattcgt ctggcccaat aacatcatga
    12841 atttccagac ggtcgttaac cctcaccgcg cggctaatcc agaactttgc agtttccaga
    12901 agtagcgcca tgccttcatg cgcaatgaaa ctttcatccc ccgtggtctg ccagtattga
    12961 ataaccgccc aggcgatatc ggccaccaga tgatgttccg cctgcgccga ggccactttt
    13021 tgccgcagcc cggtgcgaat gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg
    13081 cgcgcgcttt cccacggaaa tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc
    13141 gcgcctggca agttgtgcca gcgataacgc agtaaacttc gggcaaccgt cggatcgcta
    13201 aacagatgga acggcagcaa aaatacttct gtatcccaga aaacgtggcc tttgtagcct
    13261 tcgccggtta agccttttgc cgcaatactg ctgcgctcgc cgtgagccgg cgtcatgatg
    13321 cgcagatgat aaagcgcata atctaacgct tgctgatcgt gcgcatcgcc gccatttacc
    13381 gtgatacgac gtttctgcca ccattgacgc cagttttctg ttgatgctgc aagatgttgg
    13441 tcgtaactct gttgcgcgca cat
//
LOCUS       DADLXX010000111        13174 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLXX010000111 DADLXX010000000
VERSION     DADLXX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.112"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            19..255
                     /locus_tag="JNX27_003109"
     CDS             19..255
                     /locus_tag="JNX27_003109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884228.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(321..553)
                     /locus_tag="JNX27_003110"
                     /pseudo
     CDS             complement(321..553)
                     /locus_tag="JNX27_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
     gene            complement(663..1433)
                     /gene="yafV"
                     /locus_tag="JNX27_003111"
     CDS             complement(663..1433)
                     /gene="yafV"
                     /locus_tag="JNX27_003111"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7884229.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1587..2060
                     /gene="ivy"
                     /locus_tag="JNX27_003112"
     CDS             1587..2060
                     /gene="ivy"
                     /locus_tag="JNX27_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7884230.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2103..4547)
                     /locus_tag="JNX27_003113"
     CDS             complement(2103..4547)
                     /locus_tag="JNX27_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7884231.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4786..5364
                     /gene="lpcA"
                     /locus_tag="JNX27_003114"
     CDS             4786..5364
                     /gene="lpcA"
                     /locus_tag="JNX27_003114"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7884232.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5570..6337
                     /locus_tag="JNX27_003115"
     CDS             5570..6337
                     /locus_tag="JNX27_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7884233.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6308..7048)
                     /locus_tag="JNX27_003116"
     CDS             complement(6308..7048)
                     /locus_tag="JNX27_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7884234.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7340..7953
                     /locus_tag="JNX27_003117"
                     /pseudo
     CDS             7340..7953
                     /locus_tag="JNX27_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8129..8626
                     /gene="rayT"
                     /locus_tag="JNX27_003118"
     CDS             8129..8626
                     /gene="rayT"
                     /locus_tag="JNX27_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY7884235.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8843..10582)
                     /gene="flhA"
                     /locus_tag="JNX27_003119"
     CDS             complement(8843..10582)
                     /gene="flhA"
                     /locus_tag="JNX27_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7884236.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            10527..11312
                     /gene="lafU"
                     /locus_tag="JNX27_003120"
     CDS             10527..11312
                     /gene="lafU"
                     /locus_tag="JNX27_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7884237.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11383..12438
                     /gene="dinB"
                     /locus_tag="JNX27_003121"
     CDS             11383..12438
                     /gene="dinB"
                     /locus_tag="JNX27_003121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7884238.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12490..12783
                     /gene="yafN"
                     /locus_tag="JNX27_003122"
     CDS             12490..12783
                     /gene="yafN"
                     /locus_tag="JNX27_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY7884239.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12786..>13174
                     /gene="yafO"
                     /locus_tag="JNX27_003123"
     CDS             12786..>13174
                     /gene="yafO"
                     /locus_tag="JNX27_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY7884240.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         3186 a         3451 c         3396 g         3141 t
ORIGIN      
        1 aagggatata aagtaacaat ggaaactcat gcattatctg acgattgttt agattgtgta
       61 tatgattggt atgcggaaaa aaatggacaa ttttttagtg caacgagtcc agaagagtta
      121 cttggattag ttgcaatgtg ggaagttcgt ggtaatgact ggagagctaa tttgaatgag
      181 ataaatactt atcaaaaagc catagaagaa tccccgtttt ttgataaaaa cggcaatgaa
      241 atagagtctg actaaatttt attgatatag caacgataat aaccggaaat ttcttccaga
      301 tctttccggc tttttattat ttaactttcg ttcagcacac taaacatccc gtctcgcatc
      361 cccttaactg actgtttaac ctgataaagt tccctgcgca gttcctgtac ttcgttgtta
      421 tcccaagcag gatcaaacat ccctccgaga tcttcacggt aacgcccgtg tcgatctcaa
      481 atcccgcttt tctcagccat ttggcgctga ttttgatcac cggcgtgtcg ctgcggttgg
      541 gcagataacc cacggtacag cgacgctgtg ttttggaaat ggtgacttct ggcgtaatat
      601 tgtgtttaag ttaggggctt tcgtaatcta gcccagcccc cggccctaaa cacaaccccc
      661 actcaccaca acctaaactc atccgcatcc cgccatgccg gaaacttttc tctatattcc
      721 cgcaatgcca ccatcgacag ctccgcatca atacgcgttg ccttatgcgc gtcggcggta
      781 gcgataatct cgccttgcgg attaatcacc cggctgtcac cgcgataatg gcagccgttg
      841 ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat cgcgcgggcc
      901 gtcagcaatg cctgccagtg cagagagcgc ggagctggcc agttggcgac gtacagggcg
      961 aggtcataat cgttgagatt gcgcgaccac actggaaaac gtaagtcgta gcacaccagc
     1021 ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc tttataatgc
     1081 agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac cgtgccgccc
     1141 ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc aacactgcct
     1201 gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agttcactac gtcatcttgt
     1261 gcaagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctc cggtagaacg
     1321 atcacatcgc gcccggtaat accttccagt tgacgatcaa aatggcgcag gttggcagga
     1381 ccatccaccc acaccaacgg ttgctgcaaa agcgtaatct tcaaactagg cacggtgtac
     1441 aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt gccttagcgc
     1501 agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat tatcgtgtta
     1561 tcgccaggct ttaggaggtt aataacatgg gcaggataag ctcgggagga atgatgttta
     1621 aggcaataac gacagtcgcc gcactggtca tcgccaccag tgcaatggcg caggatgatt
     1681 taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcgtttaat cagatggtgc
     1741 aagggcataa gctacctgca tgggtgatga aaggcggtac ttatactccc gcacaaaccg
     1801 taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat gactgtggct
     1861 cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg ctgttctcgg
     1921 ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac gatgcgcttt
     1981 cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa aaccatccgg
     2041 atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc tccgttattc
     2101 aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt cgtcgccagc
     2161 tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc cgctttcacc
     2221 agaatagcgg cttcatcttt atcaatcagc cccttcgtca gcgcgttgtg cgccagttca
     2281 tccagacggg taaatggcag atttttaccc agctctttac agatccgctg atgaatcgag
     2341 tcggcggcaa tcacatccac caacgcctct tccagtaaac caaccgggtt atgctcgctt
     2401 ggcgtcaggt actgaccgcg accaatacgt gaacgagtgg cgttcggcac ttgtaaaatc
     2461 ttcgccactt tatgatccag cttgtcagac ggtgccagat aatgacgtcc ggtcgggaag
     2521 atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag taaatcgtcc
     2581 atcgcctgtt ctgcctgata cagcgcatcc tgtacgcccc agtgcaccag cggcagatcg
     2641 gcttcattac ggccttcgtc gtcataacgc ttcagaacgg cagaggcgag gtaaagctgg
     2701 cttaaaatat cccccagacg tgccgagata cgctcacgac gtttcaggct gccgcccagc
     2761 agtgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt caggtgctga
     2821 tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg cgtcaggccc
     2881 agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa caacagttta
     2941 tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac gtacggatgg
     3001 caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt agccccttca
     3061 acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct ttgcccgagc
     3121 ataatgcctt taccgccggt aatatccatc gcatcaataa tcgactgctg cccgcggtgg
     3181 gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag cataatgccg
     3241 taggtaatca gcgatgccgc agcatccatt acataagcgt tcccggcaat acgcgccagc
     3301 ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg aatgtgagaa
     3361 tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga aggcagggtg
     3421 atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc ggccattttc
     3481 ggcccgccga ttatgtaatc gatcggcacg aagacatctt taccgcgcgt cggtccgttc
     3541 tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg cgtggtggtt
     3601 gggatcagcg cacaggtaat gcctaaatct tctgcaccgc cgagtaattt ttccgggtcg
     3661 gagagtttaa acgccagccc aagcacggtc gcaatcggtg ccagcgtaat gtagcgtttg
     3721 ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat gcagacaatc
     3781 ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggctggtcag tgcaaagcag
     3841 gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc gtcagtgccg
     3901 taatgttgca acagttcgcc cgggcctaat gagtttggca cgccgacggt aatcgccagg
     3961 atcccgctca cgccagagag tttttgcagc acacgagact gggcataagc tgagaactcc
     4021 agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt aaggtacgcc
     4081 cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt cgccatccgg
     4141 caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cagcggtcag gcgcggctgc
     4201 ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc ctcccaccag
     4261 gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat caccttacgg
     4321 aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa gttaaatggc
     4381 acgaggataa tggtcagagg caccagtacc cacgccgacc acagaccagc aacgccgagg
     4441 gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg gtgatagaac
     4501 aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac gaaaagcccc
     4561 ttacttgtag gaggtctgac cactgtgatg atatggttgt agtggatgta aaaacattta
     4621 gcaatgtgtt tacaatataa ttacaacaaa gctcacattg ttgctgtttt tatccgcact
     4681 tcaggtcaaa aagtcctggt catagcacct gcccgtactt ctcgcttttg gcggtatccg
     4741 gtacactgca ttttgtctat tacatttatg ctgaaggata tcctcatgta ccaggatctt
     4801 attcgtaacg aactgaacga agcggcggaa acgctggcta actttttaaa agatgacgcc
     4861 aatattcacg ccattcagcg cgcggcggtc ctgttagcag acagctttaa agccggtggc
     4921 aaagtgcttt cctgcggcaa cggcggttcc cattgcgacg ctatgcactt tgccgaagag
     4981 ttgaccggtc gctaccgtga aaaccgtccg ggctacccgg cgattgctat ttctgacgtt
     5041 agtcatattt cctgcgtcgg taatgatttc ggtttcaatg atattttctc ccgctacgtt
     5101 gaagcggtag gtcgcgaagg cgatgtactg ctggggatct ccacctccgg taactctgca
     5161 aacgtgatca aagcgatcgc agcggcgcgt gagaagggga tgaaagtgat caccctgacc
     5221 ggtaaagacg gcggtaaaat ggctggcacg gcggatatcg aaattcgcgt tccgcacttt
     5281 ggttatgccg accgcattca ggagattcac attaaagtga tccatatcct gatccagtta
     5341 attgaaaaag agatggttaa gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat
     5401 ccggcacttg tgcgctgatg cctgatgcga tgctgacgcg tcttatcatg cctacaaatc
     5461 tgtacccgaa ccgtaggccg aataattcgt ttacgccgca tccgacctga aaattcttaa
     5521 atcaatcttc gccggggtcc atgcgctccc gctgttgtgg aggttaccca tgtgcgaatt
     5581 gctcgggatg agcgccaacg tccctaccga tatctgcttt agtttcaccg gacttgtaca
     5641 gcgtggtggt ggaaccgggc cacataaaga tggctggggc attacctttt acgaaggtaa
     5701 aggctgtcgc acatttaaag atccacaacc cagctttaat tcccccatcg ccaaacttgt
     5761 ccaggactac ccgataaaat cctgttcggt ggtggctcat attcgccagg ctaatcgggg
     5821 cgaggtggcg ctggaaaata ctcacccatt tacccgcgag ttatgggggc gtaactggac
     5881 ttatgcccat aacggacaac tgacgggcta caaatcactg gaaaccggca acttccgccc
     5941 ggtcggtgaa accgacagcg aaaaagcctt ttgctggctg ctgcataaat taacgcagcg
     6001 ttacccgcgc acgccgggca acatggcggc agtgtttaaa tatatcgcct cactggcgga
     6061 tgaactgcgg gcgaagggcg ttttcaacat gctgctttcg gacgggcgct atgtaatggc
     6121 gtattgctcg actaatttac actggatcac ccgccgcgcg ccgtttggcg tggcaacgtt
     6181 gctggatcag gatgtggaaa tcgacttcag ctcgcagacc acaccgaatg atgtggtcac
     6241 ggtgattgcg acacagccgc tgacgggcaa tgaaacctgg caaaaaatta tgccaggcga
     6301 atggcgctta ttttgcctcg gggagcgtgt agtttgatgc cagttgtggc tgcaccactt
     6361 cgtggcttaa cggcttgctg accacgtaac ggccattggc cacagaaacg gtcggtggct
     6421 tacgggtttg ctcaaagtag tcgtagcccg gcttcagctg ctcccagaag tccttaaagt
     6481 tggaatattt atggcgcttc atattggcgt cggtcatgcg gaatgggtaa atactcacct
     6541 gcacgctcgg ctgaccaaac accagcgcac cagtaacgaa ctggaagatc tcatcaatac
     6601 cctgattggt cattgcgtag cagccgatgg atacacaatc gccgtggatc atcaggtatt
     6661 tcccttcata accatgcgca cggtcataag cattggggaa tccaatatta atcgctttgt
     6721 agtaacggct gtctggtttt aactgattac gctggacgct ataaaatcct tccgggcttt
     6781 tgaaatcgcc ctgacgctgt tttggcccta agccgccgga atatttacag attttataac
     6841 tgtcgagcag ttgatattgc tcgcccattt tgacgtaaag atcgagcgta cgttcttcct
     6901 tgaagatctg gatgtagaca ggggatccca tcaactgctg cttatactct ttgctcactg
     6961 gcgtggtcga gctactgctg cccagcaaac cggcaaacga aacgcacggg atcaacaaca
     7021 tcgcaagaat taatgcgatt ttacgcatac tgcttattcc ttgataaaac ggttacacac
     7081 gccaggacgg caaaatggat cccaaatcgg aatagtctgg atttggaagg ctcacattat
     7141 caccaaaaga gttttacgca agcctgtcgg cgctgggttt acaaatttag cacaggggca
     7201 gttttcaata gtccgaaaag cgcatgtatc atttatttcc taatattatg aaatctcaat
     7261 agtaatatgc aaataagcaa ttaataatcc cgtttactcc tccggtcttt taaaattctc
     7321 accttcattc agccggttta tgtctttcat gtcttcgttc ttgctcggca gatttttgca
     7381 tccaggcgtg ttttcgttat gcgttttgct ccctttactc gccagcgcca ccacaaccgg
     7441 gagcagataa aacagcatcc agaatggttt cctgcgccgc tcaaggcgag tgacagacgc
     7501 tggcaggagc tggcggaaaa caaccacttt ttaagcagcg atcacctgca taacatcacc
     7561 gaagtggcga ttcaccgcct ggagcagcag cttggcaagc cttacgtctg gggcggcacg
     7621 cggcccgatc aaggcttcga ttgtagtggg ttagtttttt atgcctacaa caagatcctt
     7681 gaggcgaaac tcccgcgcac ggccaatgag atgtaccact accgtcgggc aacgattgtg
     7741 gcgaataacg acctgcgccg gggagatttg ctatttttcc atatccacag ccgcgagatt
     7801 gccgaccata tgggggtgta tctgggcgac ggtcagttta ttgagtcact gcgcaccggg
     7861 gaaaccattc gggtaagccg gttagccgaa cctttctggc aggaccattt tttaggcgca
     7921 cgcaggattt tgacggaaga gatgattttg taggccggat aaggcgttta cgccgcatcc
     7981 ggcggttgtg cacggatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaaatctg
     8041 tgcgtttttg cgagccgctt tcccgatata acacagcgtc attgtcggcc tttccttttc
     8101 ctcttactgt tgagaaaaag gagtcgctat gtcggaatat cgtcgttatt acatcaaggg
     8161 gggaacatgg tttttcacgg taaatttacg caatcgtcga agccatcttc tgaccaccca
     8221 gttccagacg ctccgtaacg ccattattaa cgttaagcga gacagacctt ttgaaattaa
     8281 cgcctgggtc gttttgccgg agcatatgca ttgcatatgg acattacccg aaagcgatga
     8341 tgatttttcc tctcgctggc gggagattaa aaagcagttc acccatgctt gcggattgaa
     8401 aaatatctgg caaccgcgtt tttgggagca tgccattcgc aatacaaaag attatcgcca
     8461 tcatgttgat tatatttata taaatccaga gaaacatggc tgggtaaagc aggtgagtga
     8521 ttggccattc tcaacgttcc atcgcgatgt cgcgcgaggg ttatatccca tcgattgggc
     8581 gggagacata acggatttaa gtgccgggga gcgtatcatt ttataactgt gcgctggtgc
     8641 cggatgcgct tacgcttatc cggcctacaa atcgtgcttt aacggtaggc cggataaggc
     8701 gttcacgccg catccggcag ttatgcgcag atgcctgatg cgacgctggc gcgtcttatc
     8761 atgcctaaaa acttgtgcct gaacggtagg ccggataagg cgtttacgcc gcatccggca
     8821 attgtgcacc gatgccggat tcttacatca acgcccccat aatcttcaac tccagctcat
     8881 ccggcacttc gttatacgac agcacatgca gccccggcgc aaacaacctt gcataacgcg
     8941 ccagcaaagg gcgtagctgc ggcggcacca gcagcaccgg atctttcccc gccgctttca
     9001 tctgctcttt cacctgtggc atcgtgctct ggaactggtt gagcatattc gggtcaaccg
     9061 gcacgctgtc gagcatcact ttgccggcct gttgcgcctg attcactacg ttggtcagca
     9121 gattttccag ctcattattc agcgtataca ccgtcagctc ctgcttgcga acgaacggat
     9181 gagtaatgct gcgccgcagc gccaggcgca catcggccgc cagcagaata tgatctttag
     9241 tcaccgtgct actggcgacc agcacggtgg cgatggtgac gatatcgcgc agggaaacgc
     9301 cttcggtcag cagcgcccgg tacactttca gcaattggct gtaattgagc gccgcgctta
     9361 aatcctccgc caggcgcggt gccatcgacg acaaacggtt atgcaactgc gtgatgtcat
     9421 catagttaaa caaatcagga atatagctgc gcacaatctt gttcacatgg gtggcaatca
     9481 cactggcgct gtcgatcacc tgatacccca tattcagagc cttcgctttc tgcgcaggct
     9541 ggatccaggt gaccggtatc ccgtacgccg ggtcgttccc cagcacgccg tcaatctcgc
     9601 cgtaggtttc gctggagggc agcgccatca gtttatccgc cggaatatcc gcttcatcag
     9661 ccttaatgcc gttgatgaaa atagcgtact ggctgggctt gaggcggaag ttttcccgaa
     9721 tgcggatctc cggcagcagc acgccgttac cgtcggaaat cacctgccga acgccgcgaa
     9781 tccgctgggt gagcgggttg ccctgcgctt tgtccaccag cgccaccagc ttgtagccga
     9841 ggcttaagct gatgggctcg atcagcggaa tagtttccca actgacctgt tgttcgctgg
     9901 tttcagtgat ggtgcgggtc agcgtttcga ggcttttctc ttccgcctcc gccgcctgcg
     9961 ggcgtttgct catccgccag ccggtaaagc caagcagggc gctgaacagc aaaaacggca
    10021 gatgcggcat ccccggcacc accgccagca cgaacatgat cccggtggcg gtgtagagca
    10081 ccgacgggct tgccagcaac tggtggcgca cgtcatgggc gatatcgccg ttgtcgctga
    10141 cgcgggtgac gataatcgcc gccgcggtgg agagcagcag ggaagggatc tgcgccacca
    10201 ggccgtcgcc gatggtcatc agcacgtatt gctggaaggc ggcgtcggcg ctcaggttgt
    10261 atttgaagat gccgatacag acgccgccgc tcaggttgat cgccagaatc atcatcccgg
    10321 cgatggcgtc tccgcgtaca aacttcgatg ccccgtccat cgcgccgtag aaatcggcct
    10381 cgctggcaac atctttacgc cgggtttgcg cctgcgcttg gttgatcaat ccggcgttaa
    10441 gatcggcgtc aatcgccatc tgtttgccgg gcatcgcgtc cagggtaaag cgggcagaaa
    10501 cctcggaaat acgctcggcc tctttggtga cgacgataaa gttgattgtc aacagcgtca
    10561 gtaaatccga gcgtgaaagc attatcgccg cgctgcacgg gcagtcgatt tttaacggtg
    10621 gtgggctgtc gccgttaaat aaaatcagcc cgtctcatcc gccaaaacct gccaccgtgg
    10681 cggtgccgga agagacggag aaaaaagcgc gtgatgtgaa tgagaaaacg gcgctgctga
    10741 agaagaaatc cgccaccgag cttggcgagt tggcaaccag tatcaacacc attgcccgtg
    10801 atgcgcatat ggaagcgaat ctggagatgg agattgttcc ccagggatta cgcgtgctga
    10861 ttaaagacga ccagaatcgc aatatgttcg aacgcggcag cgcgcagatt atgccgttct
    10921 ttaaaacttt gctggtggag ctcgcgccag tgttcgactc gctctataac aaaattatta
    10981 ttaccgggca taccgatgcg atggcctaca aaaacaatat ctacaacaac tggaaccttt
    11041 cgggcgatcg cgcgctttca gctcgtcggg tgctggaaga ggccggaatg ccggaagata
    11101 aagtgatgca ggtaagcgca atggcggacc agatgctgct ggacgcgaag aatccgcaaa
    11161 gcgcgggcaa tcggcgtatt gagattatgg tactgaccaa aagtgcgtcc gatacgctgt
    11221 atcaatactt tggtcagcat ggggataaag tggtgcagcc gctggtgcaa aagctggata
    11281 agcagcaggt gctttcgcag cgaatgcgct aaatgctgaa tctttacgca tttctcaaaa
    11341 cctgaaatca ctgtatactt taccagtgtt gagaggtgag caatgcgtaa aatcattcat
    11401 gtggatatgg actgcttttt cgcagcggtg gagatgcgcg acaatcccgc tctgcgcgat
    11461 atccctattg ctattggcgg cagccgcgaa cgtcgggggg tgatcagcac cgccaattat
    11521 cccgcgcgta aatttggcgt acgtagcgct atgcctactg ggatggcgct caaattatgc
    11581 ccacatctca ccttgcttcc ggggcgcttt gacgcctaca aagaagcctc aaatcatatc
    11641 cgtgaaatct tctcgcgcta cacctcgcgc attgaaccgt tgtcactgga tgaggcttat
    11701 ctcgatgtca ccgatagcgt ccattgccac ggttctgcga ccctcatcgc ccaggaaatc
    11761 cgccagacga tcttcaacga gctgcaactg acggcatctg cgggcgtggc accagtaaag
    11821 tttctcgcca aaatcgcctc cgacatgaat aaacccaacg gtcagtttgt gattacgccg
    11881 gcagaagttc cggcattttt acaaacctta ccactggaaa aaatccccgg cgtcggcaaa
    11941 gtctcggcgg caaaactgga agcgatgggg ctgcggacct gcggtgatgt acaaaagtgt
    12001 gatctggtga ttctgcttaa acgctttggc aaatttggcc gcattttgtg ggagcgtagt
    12061 caggggattg acgagcgcga cgttaacagc gaacggttac gaaaatccgt cggcgtggaa
    12121 cgcacgatgg cggaagatat tcatcactgg tctgaatgtg aagcgattat cgagcggctg
    12181 tatccggaac ttgaacgccg tctggcaaag gtaaaacctg atttactgat tgctcgccag
    12241 ggggtgaaat taaagttcga cgattttcag cagaccaccc aggagcacgt ctggccgcgg
    12301 ctgaataaag ctgacttaat cgccaccgcg cgtaaaacct gggatgaacg ccgcggcggg
    12361 cgcggtgtgc gtctggtggg gctgcatgtg acgttgcttg acccgcaaat ggaaagacaa
    12421 ctggtgctgg gattatgatg tatactatta tgtatattct ggtgtgcatt attatgaggg
    12481 tatcactgta tgcatcggat tctcgctgaa aaatcggtca atatcactga gttacgtaaa
    12541 aacccagcta aatactttat tgatcaacca gttgcggttc tttctaataa tcgccccgca
    12601 ggatatctct taagtgccag cgcattcgaa gcgttaatgg acatgcttgc tgaacaagag
    12661 gagaaaaagc ccataaaggc gcgctttcgt ccaagtgctg caagattaga ggaaattaca
    12721 cgccgcgcta aacaatatct taatgatatg acggatgatg atttcaatga ctttaaggaa
    12781 taaggatgcg ggtattcaaa acaaaactta ttcgcctgca acttacagca gaggaacttg
    12841 atgcgttaac ggcggatttt atttcctata agcgtgacgg tgttttgcca gatatatttg
    12901 gtcgcgatgc actctacgac gactcgttta cctggccatt aatcaaattt gagcgagttg
    12961 ctcatattca tctggcaaat gtgaataatc catttccgcc acagttgcgc caattcagca
    13021 gaacgaatga cgaagcgcat ttggtatatt gtcagggtgc gtttgatgag caagcatggt
    13081 tgctcattgc cattctgaag cctgaacctc ataaactggc tcgagataac aaccaaatgc
    13141 ataaaattgg aaaaatggca gaagcgtttc gcat
//
LOCUS       DADLXX010000112        13157 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLXX010000112 DADLXX010000000
VERSION     DADLXX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.113"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(67..2016)
                     /locus_tag="JNX27_003124"
     CDS             complement(67..2016)
                     /locus_tag="JNX27_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7884241.1"
                     /translation="MTDSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="JNX27_003125"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="JNX27_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="JNX27_003126"
     CDS             complement(2437..2793)
                     /locus_tag="JNX27_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884242.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="JNX27_003127"
     CDS             complement(2793..4289)
                     /locus_tag="JNX27_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884243.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="JNX27_003128"
     CDS             complement(4286..4447)
                     /locus_tag="JNX27_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7884244.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5019)
                     /locus_tag="JNX27_003129"
                     /pseudo
     CDS             complement(4457..5019)
                     /locus_tag="JNX27_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5016..5540)
                     /locus_tag="JNX27_003130"
     CDS             complement(5016..5540)
                     /locus_tag="JNX27_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884245.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRYARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="JNX27_003131"
     CDS             complement(5506..5922)
                     /locus_tag="JNX27_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7884246.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="JNX27_003132"
     CDS             complement(5919..6242)
                     /locus_tag="JNX27_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7884247.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..8017)
                     /locus_tag="JNX27_003133"
     CDS             complement(6287..8017)
                     /locus_tag="JNX27_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7884248.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGTTSGWVG
                     ETEARQETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7995..9236)
                     /locus_tag="JNX27_003134"
     CDS             complement(7995..9236)
                     /locus_tag="JNX27_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7884249.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="JNX27_003135"
     CDS             complement(9236..9418)
                     /locus_tag="JNX27_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884250.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="JNX27_003136"
     CDS             complement(9430..11187)
                     /locus_tag="JNX27_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7884251.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEEWGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="JNX27_003137"
     CDS             complement(11187..11669)
                     /locus_tag="JNX27_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7884252.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="JNX27_003138"
     CDS             complement(11817..12167)
                     /locus_tag="JNX27_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7884253.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..12674)
                     /locus_tag="JNX27_003139"
     CDS             complement(12210..12674)
                     /locus_tag="JNX27_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7884254.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(12671..12988)
                     /locus_tag="JNX27_003140"
                     /pseudo
     CDS             complement(12671..12988)
                     /locus_tag="JNX27_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900875.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
BASE COUNT         2970 a         3884 c         3051 g         3252 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agtcatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacccggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggagtgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatatgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcaggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcataa cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttcctg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccacccagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgccca ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacatcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat cccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgatgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccactcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcgcccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtctctgc tgcatgtcag taatggttgc gttcgccagc
    12541 ttcagttctc ttgcgttttt gtcgcgctgc tctttgtagg taatggcgtt atcacggtaa
    12601 tgattaacag cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg
    12661 gtgactctgc tcattgctgc ccccacaaac agacttcacg ctcaatctca cgacgggtca
    12721 tcagcccttt ccattgctta ccgccagcat atgtccagcg acgtagctgg tcacatgcgc
    12781 ctttgatatc gccctagttt attttgcgaa gaagcatcga tgttctgaaa ttgccagcgc
    12841 ccacgttgta aacgaacgag taaagagcgc cgcgcgttgt ttccggtata tcgactttga
    12901 tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt attcaggagg gctttgcatt
    12961 ctgcttcggt atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg tggcatacag
    13021 tccatacgcc aacgatatct ttgtatggta tgtagctgac accttccaga ccatcgttac
    13081 cacctgggcc agtgatgagc acagacgcta tggcaatagc cccaccacca atagcaactg
    13141 caacagcctt gcgtaat
//
LOCUS       DADLXX010000113        12963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLXX010000113 DADLXX010000000
VERSION     DADLXX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.114"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            79..867
                     /gene="modE"
                     /locus_tag="JNX27_003141"
     CDS             79..867
                     /gene="modE"
                     /locus_tag="JNX27_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7884255.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNAATSLQQGQNVTAYFNADSVIIATLC"
     gene            935..2407
                     /gene="modF"
                     /locus_tag="JNX27_003142"
     CDS             935..2407
                     /gene="modF"
                     /locus_tag="JNX27_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7884256.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2668..3684
                     /gene="galE"
                     /locus_tag="JNX27_003143"
     CDS             2668..3684
                     /gene="galE"
                     /locus_tag="JNX27_003143"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7884257.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3694..4740
                     /gene="galT"
                     /locus_tag="JNX27_003144"
     CDS             3694..4740
                     /gene="galT"
                     /locus_tag="JNX27_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7884258.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4744..5892
                     /gene="galK"
                     /locus_tag="JNX27_003145"
     CDS             4744..5892
                     /gene="galK"
                     /locus_tag="JNX27_003145"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7884259.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5886..6926
                     /gene="galM"
                     /locus_tag="JNX27_003146"
     CDS             5886..6926
                     /gene="galM"
                     /locus_tag="JNX27_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7884260.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDEQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7129..7881
                     /gene="gpmA"
                     /locus_tag="JNX27_003147"
     CDS             7129..7881
                     /gene="gpmA"
                     /locus_tag="JNX27_003147"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7884261.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8048..9100)
                     /gene="aroG"
                     /locus_tag="JNX27_003148"
     CDS             complement(8048..9100)
                     /gene="aroG"
                     /locus_tag="JNX27_003148"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7884262.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAIRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9416..9796
                     /locus_tag="JNX27_003149"
     CDS             9416..9796
                     /locus_tag="JNX27_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884263.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9910..10851
                     /gene="zitB"
                     /locus_tag="JNX27_003150"
     CDS             9910..10851
                     /gene="zitB"
                     /locus_tag="JNX27_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7884264.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10848..11567)
                     /locus_tag="JNX27_003151"
     CDS             complement(10848..11567)
                     /locus_tag="JNX27_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7884265.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11605..12648)
                     /gene="nadA"
                     /locus_tag="JNX27_003152"
     CDS             complement(11605..12648)
                     /gene="nadA"
                     /locus_tag="JNX27_003152"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7884266.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
BASE COUNT         3235 a         3378 c         3263 g         3087 t
ORIGIN      
        1 aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc acaataagac
       61 ttttgccagg acattgttat gcaggccgaa atccttctca cccttaagct ccaacaaaaa
      121 ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc cggttccatt
      181 agccagggag cgaaagatgc cggtatcagc tataaaagcg cctgggatgc cattaacgag
      241 atgaatcagt taagtgagca tattctggtc gagcgcgcaa caggcggtaa aggtggcgga
      301 ggcgcagtac tgacccgcta tggtcagcga ctgattcagc tctatgactt actggcgcaa
      361 atccagcaaa aagcctttga tgtgttaagt gatgatgacg ccctgccgct gaacagcctg
      421 ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccagtg gttcggtacc
      481 atcaccgccc gcgatcatga tgacgttcaa cagcatgttg atgtcttact ggctgacgga
      541 aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cacgtctggg gctggatgaa
      601 ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag gcattactca ggacgaagcg
      661 gtcgcacaaa acgccgacaa ccaattaccg ggtattatta gtcatattga gcgcggcgca
      721 gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc cacagtgccg
      781 gtaaatgcag cgacttctct tcagcaaggg cagaatgtca cggcctactt taatgccgac
      841 agcgtgatta tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa acacgtatcc
      901 ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa ttttgcaagg
      961 cacgtttcgt cttagcgaca caaaaacgct gcaattgcct cagctaacgt taaacgcggg
     1021 tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag tcggcgctgg cccgcgcgct
     1081 ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc acatcactcg
     1141 tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcaacgga ataacaccga
     1201 tatgctcggc cctagcgaag atgacaccgg acgcactacg gctgagatta ttcaggatga
     1261 agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta ccgccctcct
     1321 cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc tgtgtcaggc
     1381 gctgatgtcg gagcctgact tgttgattct tgatgagccg ttcgatggcc tggatgttgc
     1441 ctcacgtcag cagctggctg agcgactcgc ctcgttacat cagtccggta ttactttggt
     1501 actggtgctc aatcgcttcg ataagatccc ggaatttgtc cagtttgctg gcgtgctggc
     1561 ggattgtacg ttagcggaaa ctggcgctaa agaggaactg ctccagcaag cactcgtcgc
     1621 gcaacttgcg catagtgaac agcttgaagg tgtgcaactg ccggagccgg atgaaccttc
     1681 agcacgtcac gccttacccg ccaacgaatc gcgcattgtg ctgaacaatg gcgtggtttc
     1741 ttataatgat cgccccattc ttaataacct tagctggcag gtgaatccag gcgaacactg
     1801 gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga ttactggcga
     1861 tcatccgcaa ggttacagca acgatttgac gcttttcggc cgacgtcgcg gcagcagcga
     1921 aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc agtagtttgc atctggatta
     1981 ccgggtcagc actaccgtgc gtaatgtgat tctttctggc tattttgatt cgattggcat
     2041 ttatcaggcc gtttcggatc gccagcaaaa actggtgcag cagtggctgg atattctcgg
     2101 cattgataaa cacacggctg acgctccgtt ccatagtctt tcctggggac agcagcgtct
     2161 ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg cttattctcg atgaaccact
     2221 acaggggctt gatccgctca atcgccaact tatccgccgt tttgttgatg tgctgattag
     2281 cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct gaagatgcgc ctgcctgtat
     2341 tacccatcgc cttgagttcg tgccggacgg tggactctat cgctatgtgc tgacaaaaat
     2401 atattgagtc ggtagtgctg accttgccgg aggcggccta agcgccctct ccggccaacg
     2461 gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga taaaaagcgc aatcattcat
     2521 aaaccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt cttgtgtaaa
     2581 cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct atggttattt
     2641 cataccataa gcctaatgga gcgaattatg agagttctgg ttaccggtgg tagcggttac
     2701 attggaagtc atacctgcgt gcaattactg caaaacggtc atgatgtcat cattcttgat
     2761 aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg cggcaaacat
     2821 ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga tgaccgagat cctgcacgat
     2881 cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgtcgccga atcggtacaa
     2941 aaaccgctgg aatattacga caacaatgtc aacggcactc tgcgcctgat tagcgccatg
     3001 cgcgccgcta acgtcaaaaa ctttattttt agctcctccg ccaccgttta tggcgatcag
     3061 ccccaaattc catacgttga aagcttcccg accggcacac cgcaaagccc ttatggcaaa
     3121 agcaaattga tggtggaaca gatcctcacc gacctgcaaa aagcccagcc ggactggagc
     3181 attgccctgc tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga tatgggcgaa
     3241 gatccgcaag gcattccgaa taacctgatg ccatacatcg cccaagttgc tgtaggccgt
     3301 cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagatggtac tggcgtacgc
     3361 gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga aaaactggcg
     3421 aacaagccag gcgtacacat ctacaacctc ggtgctggcg taggcagcag cgtgctggac
     3481 gtggttaatg ccttcagcaa agcctgcggc aaaccggtta actatcattt tgcaccgcgt
     3541 cgcgaaggcg accttccggc ctactgggcg gacgccagca aagccgaccg tgaactgaac
     3601 tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg gcagtcacgc
     3661 catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc cgttgatcat
     3721 ccacatcgcc gctacaaccc gctcaccggg caatggattc tggtttcacc gcaccgcgct
     3781 aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc tgcgcacgat
     3841 ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa ccccgattac
     3901 accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac gccagatgcg
     3961 ccagaaagca acgatccttt gatgcgttgc cagagcgcgc gcggtaccag ccgggtgatc
     4021 tgcttttcac cggatcacag taaaacgttg ccagaactga gcgttgcggc attgacggaa
     4081 atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa aaacgtaccc gtgggtgcag
     4141 gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca cggacagatt
     4201 tgggcaaata gcttcctgcc taacgaagct gagcgcgaag accgcctgca aaaagaatat
     4261 tttgccgaac agaaatcacc aatgctggtg gattatgttc agcgcgagct ggcagacggt
     4321 agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg tgccttactg ggctgcctgg
     4381 ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc ggatcaccga tttgaccgac
     4441 gcccagcgca gcgatttggc gctggcgttg aaaaagctga ccagtcgtta tgacaacctc
     4501 ttccagtgct ccttccccta ctctatgggc tggcacggcg caccatttaa tggcgaagag
     4561 aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgctgcgctc cgccaccgta
     4621 cgtaaattta tggttggtta tgaaatgctg gcagagaccc agcgagacct gaccgcagaa
     4681 caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc cggagtgtaa
     4741 gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg ctaccctgcc
     4801 actcacacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac cgactacaac
     4861 gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg tgcaccacgc
     4921 gatgaccgta aagttcgcgt gatggcagcc gattatgaaa atcagctcga cgagttttcc
     4981 ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt tcgtggcgtg
     5041 gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt gatcagcggc
     5101 aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc ggtcggaacc
     5161 gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct taacggtcag
     5221 gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct aatttccgca
     5281 ctcggcaaga aagatcatgc cttgctgatt gactgccgct cactggggac taaagcagtt
     5341 tccatgccga aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg taccctggtt
     5401 ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt cttccagcag
     5461 ccagctctgc gcgatgtcac cattgaagag ttcaacgctg ttgcacatga gctggaccca
     5521 atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt tgaagctgcc
     5581 agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt tgatggcgga gtctcatgcc
     5641 tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt agaaatcgtc
     5701 aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt tggcggctgt
     5761 atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt cgctgaacaa
     5821 tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc acaaggagca
     5881 ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac cgactgttaa
     5941 ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg actttacttt
     6001 ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgctcggc tgtgccagtc
     6061 cggaatgcta tcaggatcag gctgcgtttc tgggggcctc tattggtcgt tataccaacc
     6121 gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt gacgctttcg ccaagccagg
     6181 gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg cagattgtga
     6241 accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat cagggcttcc
     6301 cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt atctccatta
     6361 cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac gtctatttca
     6421 atcttgacgg cgagcagtct gacgtgcgca atcacaagtt gcagattctg gcggacgaat
     6481 atcttccggt tgatgaaggc ggcattccgc acgacggctt gaaatctgtc gccggaacgt
     6541 cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac gacgagcagc
     6601 gcaaagtgaa aggttacgat cacgcattct tgttacaggc caaaggcgat ggcaagaaag
     6661 tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac accaccgctc
     6721 cggctcttca attctactcc ggcaacttcc tcggcggcac accgtcgcgg ggaaccgaac
     6781 cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac agcccgaacc
     6841 accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg cgaagagtat tccagcctga
     6901 cggaatatca gtttattgct gagtaatgtt aagccctcgt catgagggct ttatcttcat
     6961 attgttcaaa tcaccagcaa acaccgacat atttgcaact caatattcac aataacctta
     7021 cactgcgcca ctattttcgc tatggttatg cgtaagcatt gctgttgctt cgtcacggca
     7081 atataatgag aattattatc attaaaagat gatttgagga gtaagtatat ggctgtaact
     7141 aagctggttc tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg tttcaccggt
     7201 tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc aggtaagctg
     7261 ctgaaagagg aaggttacag ctttgacttt gcttacactt ctgtgctgaa acgcgctatc
     7321 cataccctgt ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt tgagaaatcc
     7381 tggaaactga acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc ggaaactgct
     7441 gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt gactccgccg
     7501 gaactgacta aagatgatga gcgttatccg ggtcacgatc cgcgttacgc gaaactgagc
     7561 gagaaagaat tgccgctgac ggaaagcctg gcgctgacca ttgaccgcgt gatcccttac
     7621 tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc gcgtaatcat cgccgcacac
     7681 ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca tgagcgaaga agagattctt
     7741 gagctgaata tcccgactgg cgtgccgctg gtgtatgagt tcgacgagaa tttcaaaccg
     7801 ctgaaacgct attatctggg taatgctgac gagatcgcgg cgaaagcagc agccgttgcg
     7861 aaccagggta aagcgaagta aacgtcattc gtttaaaaaa tgagaaagcc gactgcaagt
     7921 gagtcggctt ttttgtttgt cgttgctaac aatggagcac attgcctgat gcgacgctgc
     7981 gcgtcttatc aggcctgtgg tgattcatcg gatacgccac tctgacggcg catccgacaa
     8041 ttaaacctta cccgcgacgc gcttttactg cattcgccag ttgacgtaac agagcatcgg
     8101 tatcttccca gccaatgcag gcatcggtga tgctcttacc gtaggccagc ggctccccgc
     8161 tatcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca ataatggcct
     8221 tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac atccatctgc tttttgaatt
     8281 gtttggacga attagcatgg ctgaaatcga tcatcacctg agctggcaga cctgctttgt
     8341 tcagcccttc tttcacttca gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc
     8401 gcagaatgat atggcaatcg ccgttaccac tggtattcac aatcgccgaa tgcccccatt
     8461 tagttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg atagccactt
     8521 taatcgtacc gtctgtgcca tttttaaaac ctaccggaca agaaagacca gacgccagtt
     8581 cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc gccccagctc atcaggtcag
     8641 cgagatactg aggagtgatc atatcgagaa actcacccgc cgctggcaga ccgctgtcgt
     8701 taatatcaag cagcaattta cgggctatac gcagaccgtc gttgatctgg aagctgttat
     8761 ccatatgcgg atcgttaatc aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat
     8821 agacgcgcat tacgatttcc agctcatctt tcagctcttc acgcagcgcc agcaagcgaa
     8881 tggcatactc tttagccgcg acaggatcat gaattgagca cgggccaatc accaccaaca
     8941 ggcgatcatc attacctttc aggatcttat ggatcgcttt tcgggcatga gcaaccgtat
     9001 tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc gacaggagga agtaactctt
     9061 tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca gtgtcgccat
     9121 acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg tgtaaacggg
     9181 gttttacact atgaacggat taatcctaga attagcaaga aaaacgccag attgtcgcga
     9241 aaaacgagat ctttcctaca atttctaact gtaactcctt tgtttgttaa ttatttcaag
     9301 attctctgct gcgtttcata aactggctga aatcttaaac caatgcctta tattcacctg
     9361 caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc atcctatgaa
     9421 aatgacaaaa ctggccacat tatttctgac tgccactcta agccttgcca gcggtgccgc
     9481 actggctgcc gatagcggag cgcaaactaa taacggccag gcaaacgccg cagctgatgc
     9541 gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg
     9601 ggtaaatacc ggttctggcg gcacaatgct gcattcggat ggttcttcaa tgaacaatga
     9661 cggaatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc gatgtccgga
     9721 tatcaataaa aaggtacaaa ccggtgatgg catcaataat gatgtcgata ccaaaaccga
     9781 cggtaccacg cagtaaccct gcaaaatagc caataatggg agagcggagt ctctcccttt
     9841 taatttttca cttctaaaag cagtatgatg tcataaagtt aagtgagaat aattaaggga
     9901 tgactcgtca tggcgcactc acactcacac acgtcctcac acctgccaga agataataat
     9961 gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctggt agaagtcgtt
    10021 ggtggttttc tttctggttc tctggcattg ctggccgatg cgggtcatat gttgaccgat
    10081 actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt
    10141 cgccacactt tcggttggct aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc
    10201 ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag aacgcttccg cacgccgcgt
    10261 ccggtcgagg gaggcatgat gatggcaatt gccgtggctg ggttgctggc aaatatactt
    10321 tctttctggt tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca
    10381 ctgcatgtgc tcggggacct gctgggttcg gttggggcta ttattgccgc gttgattatt
    10441 atctggactg gctggacacc tgctgacccc attctctcga tactggtgtc gcttctggtt
    10501 ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga aggtgcaccg
    10561 gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc
    10621 aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgatgac gttgcatgtg
    10681 caggtgatcc cgccacacga tcacgatgcc ttgttggatc agatccaaca ctacctgatg
    10741 gatcactatc agattgagca cgccaccatt cagatggaat atcaaccttg tcatgggcca
    10801 gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta atgggacagc
    10861 gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc ggctgccgtt gagcgcaata
    10921 aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg
    10981 acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt acgcgtcatc
    11041 agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg gaaagcgtcc
    11101 ggttgcagtt caggcatcac aacctgtaat cctaatgctt gcatgatcat gactgccacg
    11161 cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat cgaaacaacg
    11221 caaaccgcca accagctgag tgccttcggc aatggcaacc agcgaatttt caactccgcc
    11281 tcgttctgac tggtttgtcg cgaccacgca taccagccgt aaatattcgc ggcaaagaaa
    11341 aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat gccaaacaag
    11401 gtaacgttga tcaggccaaa gaagtagttg ctgatcttct ccagactggc aaggccaata
    11461 cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata accgcccgcc
    11521 cctattggta tatgtaccag gatattctgc acactaaaaa aatccatctt tcccccaaag
    11581 cgtaacgtta cgccttatta ttcgttatcc acgtagtgta accgcaaaat ccagcatacg
    11641 attgagcggc accagcgccc tctctcgcag cctctcatcg acatgaacct cgtgattact
    11701 tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca ttcatggcca tccacggaca
    11761 atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt ggtgcttcca gtaactcttt
    11821 atctggcacc gcctgctgca ttttgtagaa aataccccga tcggttgcca caataagcct
    11881 ctgatgaggc aatgcttttg cagcagcgat cagttgactg gtggaaccga ccgcatccgc
    11941 catatcgaca atagcttgtg gtgattctgg atgcaccagt atggcagcat ccgggtattc
    12001 ttcttgcaag cgggttaacg cctgagtctt aaattcatca tgcacaatgc aggcaccctg
    12061 ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa cgccccagat gtttgtcggg
    12121 tgcccagatg attttttcac ccaaactatc aagatgatca ataagttcga cggcaatgct
    12181 tgaagttacc acccaatctg cgcgcgtttt taccgcagca gaagtgttgg cgtagacgac
    12241 gacagtacga tcgggatggg catcgcaaaa tgcgttaaat tcttcaacag ggcagccgag
    12301 atccagtgaa cactcaacct gaagtgtcgg catcagaatt gttttttccg gactgagaat
    12361 tttggcggtt tctcccataa atctcacccc agcgactaac aaagtagaag cgggatgctt
    12421 tgcaccgaag cgcgccattt ccagagaatc agaaatacag ccaccggttt cttctgccag
    12481 ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc accgcattac gttcttttag
    12541 cagacgtttt atcttctcgc ggtaatacgc tttttcatca atgcttaacg gcgtcggctt
    12601 cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt acgctcatct taccatctcg
    12661 ttttacaggc ttaacgttaa aaccgacatt agcgtaacat tcgcttttat cggtgtaatt
    12721 tgttttatat gctaaacaag atagtcgaat ggtggggaaa agtcactcga attacgccta
    12781 attccaccga cgtagagttg cttgcacagt aagctctgac gatgtcatca ggtttacaaa
    12841 gtgaaattga ggatgcctga atagcaaaaa agcgccttta gggcgctttt ttacattggt
    12901 gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg
    12961 agc
//
LOCUS       DADLXX010000114        12885 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLXX010000114 DADLXX010000000
VERSION     DADLXX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.115"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(511..1602)
                     /gene="ychF"
                     /locus_tag="JNX27_003153"
     CDS             complement(511..1602)
                     /gene="ychF"
                     /locus_tag="JNX27_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7884267.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1719..2303)
                     /gene="pth"
                     /locus_tag="JNX27_003154"
     CDS             complement(1719..2303)
                     /gene="pth"
                     /locus_tag="JNX27_003154"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7884268.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2581..2859
                     /gene="ychH"
                     /locus_tag="JNX27_003155"
     CDS             2581..2859
                     /gene="ychH"
                     /locus_tag="JNX27_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7884269.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="JNX27_003156"
     CDS             complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="JNX27_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7884270.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4718..5665)
                     /locus_tag="JNX27_003157"
     CDS             complement(4718..5665)
                     /locus_tag="JNX27_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7884271.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="JNX27_003158"
     CDS             complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="JNX27_003158"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7884272.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="JNX27_003159"
     CDS             complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="JNX27_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7884273.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7504..8760
                     /gene="hemA"
                     /locus_tag="JNX27_003160"
     CDS             7504..8760
                     /gene="hemA"
                     /locus_tag="JNX27_003160"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7884274.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8802..9884
                     /gene="prfA"
                     /locus_tag="JNX27_003161"
     CDS             8802..9884
                     /gene="prfA"
                     /locus_tag="JNX27_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7884275.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9884..10717
                     /gene="prmC"
                     /locus_tag="JNX27_003162"
     CDS             9884..10717
                     /gene="prmC"
                     /locus_tag="JNX27_003162"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7884276.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10714..11106
                     /locus_tag="JNX27_003163"
     CDS             10714..11106
                     /locus_tag="JNX27_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7884277.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11110..11919
                     /locus_tag="JNX27_003164"
     CDS             11110..11919
                     /locus_tag="JNX27_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY7884278.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEEPISEMIRAQINNIAHKHIVLH"
     gene            11955..12809
                     /gene="kdsA"
                     /locus_tag="JNX27_003165"
     CDS             11955..12809
                     /gene="kdsA"
                     /locus_tag="JNX27_003165"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7884279.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3229 a         3233 c         3332 g         3091 t
ORIGIN      
        1 attttcgctt gatgtatctg cataacaaaa acaatatgcg ccatttttgc attactaata
       61 agaaaaagca aaaaacatcg atattcattt ttggtcaatg gattttctgg cctatcgtta
      121 ttattgtgct actggttatc attttcatct tgccaggatc gcacgtaatc acgttattcg
      181 gggactttat ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga
      241 gcgagatcaa tattgatcag ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg
      301 ttatatcact gctaatgaga tatcagtcat ctgaacactt atgtctttaa ttattctcgt
      361 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      421 tgaaatgaaa aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat
      481 ttagcgaaac ctcatgagac aataaattaa ttagacgttg aaaaggaagt tcatcacatc
      541 gccatctttc acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      601 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      661 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      721 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      781 ataaccggca cggatcacac ggttcaggcc cggttcttcc agcccaagct cctgcataaa
      841 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      901 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      961 ttcaaaaccg tcttcgttga cgttggctat gtacattgtt ggtttcagcg tcaggaagct
     1021 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
     1081 gttttccagc tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
     1141 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1201 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1261 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1321 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1381 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1441 cagttgatcc aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca
     1501 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1561 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1621 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1681 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1741 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1801 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1861 ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1921 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1981 cccaacttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     2041 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     2101 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagggtgac tcgcgaagta
     2161 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2221 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2281 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2341 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2401 attgcctgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2461 tgaaataagg gttgtaattg tgattacgcc cgcacataac ccacagggtg ttgtctatac
     2521 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2581 atgaaacgca aaaacgcttc gatactcggt aacgtgctca tggggttggg tctggtggta
     2641 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2701 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2761 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg tcactatgac
     2821 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2881 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2941 attcgggaag aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag ccatagttcg
     3001 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccttcgggca gacgcttcac
     3061 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag
     3121 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaataagc cttcagcagc
     3181 agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac
     3241 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3301 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagtgaca tacacagcag
     3361 catgacgatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc
     3421 actcatgttc cacgccacca tcaacagcag ggctgccatg gcggaaagcg gcagccagga
     3481 gagcagtggt gccagtacca gcagggcgag aataaccaga atagagtgga tcaccgccga
     3541 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc
     3601 ggtaatacca ccaaagaatg gagcgatgat attccccagc ccctgaccga ccagttcact
     3661 attcgccttg tgcttagtcc cggtcatacc atccagtacc acagcgcaga gcagagattc
     3721 gatcgccccg agcattgcca ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca
     3781 ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg
     3841 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3901 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3961 cggaaggtga ccgggtaaac gaatgcccag acgcggccag aaaaccagaa tgccaagcgt
     4021 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     4081 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     4141 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4201 aatatactca atcagacgac caaagcgtgc cagccccatc agaatcaaaa agattcccga
     4261 caacaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgaaacgg gatagagaat
     4321 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gacccacccg ttagagcaat
     4381 gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat
     4441 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4501 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4561 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4621 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4681 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4741 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4801 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4861 cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag
     4921 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag cgcttcagca gctttacaca
     4981 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     5041 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     5101 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     5161 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5221 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5281 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5341 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5401 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5461 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5521 gtacattttc gttaatttgt acgctgactt cgccgtcgct aaagcgacct acagcggcgt
     5581 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagtaccggg gtggcgttac
     5641 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5701 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc
     5761 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5821 gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt
     5881 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5941 aggcccctgt cccagtcagg cgcgacgggg cgtattttaa cagccaggaa agcaccgcat
     6001 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     6061 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     6121 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6181 ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6241 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6301 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6361 cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac
     6421 gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt
     6481 catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa
     6541 tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6601 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6661 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6721 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac
     6781 cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag
     6841 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6901 aattggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6961 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct
     7021 gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa
     7081 gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg
     7141 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc
     7201 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7261 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7321 gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat
     7381 tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc
     7441 ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca
     7501 gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa
     7561 cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg
     7621 atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt
     7681 gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7741 aatgaagaag atctgcgtaa aaacctctac tggcatcagg ataacgacgc ggttagccat
     7801 ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt
     7861 caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa
     7921 cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt
     7981 gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     8041 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     8101 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     8161 caaattctgg cagatgaagt tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt
     8221 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8281 ggcatggtgg agcgcgcatt aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt
     8341 gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagt
     8401 gtggacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8461 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8521 agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcacgttcg cgatgagtta
     8581 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8641 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8701 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8761 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8821 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8881 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8941 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     9001 tgatgctcga cgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     9061 aaaaaagcga gcaactggaa cagcaattac aggttctatt actaccaaaa gatcctgatg
     9121 acgaacgtaa cgccttcctc gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt
     9181 ttgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag
     9241 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9301 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg
     9361 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9421 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9481 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9541 ctcacttgcc gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca
     9601 aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc
     9661 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9721 accgtactta caatttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9781 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9841 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9901 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9961 cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca
    10021 gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
    10081 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttctcc
    10141 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10201 gcctgaacaa ccttgccgta ttctcgatct cgggacgggt accggggcga ttgcgcttgc
    10261 gctggctagc gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt
    10321 ctccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10381 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10441 tattgacgag caggacccac atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10501 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10561 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10621 gcgacaagca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10681 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10741 gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10801 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10861 acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc
    10921 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10981 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    11041 ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    11101 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    11161 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11221 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11281 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11341 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11401 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11461 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11521 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11581 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11641 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11701 gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa
    11761 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11821 ttaagttatt tcgttgaaca gtgtccggaa gagccgatca gcgaaatgat ccgtgcgcaa
    11881 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11941 attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    12001 acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc
    12061 gcatttgcga gcactacgta actgtgaccc agaaactggg tatcccttac gtgttcaaag
    12121 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12181 aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg
    12241 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12301 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12361 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12421 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca
    12481 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12541 tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca gcttccggtg
    12601 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12661 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12721 cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag
    12781 gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg
    12841 ttctgcgtta agactttttt catgggtgcc agatacaaaa aaggc
//
LOCUS       DADLXX010000115        12749 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLXX010000115 DADLXX010000000
VERSION     DADLXX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.116"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(101..739)
                     /gene="rutR"
                     /locus_tag="JNX27_003166"
     CDS             complement(101..739)
                     /gene="rutR"
                     /locus_tag="JNX27_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY7884280.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1027..2118
                     /gene="rutA"
                     /locus_tag="JNX27_003167"
     CDS             1027..2118
                     /gene="rutA"
                     /locus_tag="JNX27_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY7884281.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPSAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2118..2810
                     /gene="rutB"
                     /locus_tag="JNX27_003168"
     CDS             2118..2810
                     /gene="rutB"
                     /locus_tag="JNX27_003168"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY7884282.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2822..3208
                     /gene="rutC"
                     /locus_tag="JNX27_003169"
     CDS             2822..3208
                     /gene="rutC"
                     /locus_tag="JNX27_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY7884283.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3216..4016
                     /gene="rutD"
                     /locus_tag="JNX27_003170"
     CDS             3216..4016
                     /gene="rutD"
                     /locus_tag="JNX27_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY7884284.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4026..4616
                     /locus_tag="JNX27_003171"
     CDS             4026..4616
                     /locus_tag="JNX27_003171"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY7884285.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4627..5121
                     /gene="rutF"
                     /locus_tag="JNX27_003172"
     CDS             4627..5121
                     /gene="rutF"
                     /locus_tag="JNX27_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY7884286.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5142..6470
                     /gene="rutG"
                     /locus_tag="JNX27_003173"
     CDS             5142..6470
                     /gene="rutG"
                     /locus_tag="JNX27_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY7884287.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6552..6659)
                     /locus_tag="JNX27_003174"
     CDS             complement(6552..6659)
                     /locus_tag="JNX27_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884288.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7032..7628
                     /gene="wrbA"
                     /locus_tag="JNX27_003175"
     CDS             7032..7628
                     /gene="wrbA"
                     /locus_tag="JNX27_003175"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7884289.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7649..7876
                     /locus_tag="JNX27_003176"
     CDS             7649..7876
                     /locus_tag="JNX27_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884290.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7914..9155)
                     /gene="agp"
                     /locus_tag="JNX27_003177"
     CDS             complement(7914..9155)
                     /gene="agp"
                     /locus_tag="JNX27_003177"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY7884291.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9448..10704)
                     /locus_tag="JNX27_003178"
     CDS             complement(9448..10704)
                     /locus_tag="JNX27_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY7884292.1"
                     /translation="MGRNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10965..11885
                     /gene="cbpA"
                     /locus_tag="JNX27_003179"
     CDS             10965..11885
                     /gene="cbpA"
                     /locus_tag="JNX27_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7884293.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11885..12190
                     /gene="cbpM"
                     /locus_tag="JNX27_003180"
     CDS             11885..12190
                     /gene="cbpM"
                     /locus_tag="JNX27_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7884294.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12283..12699)
                     /gene="torD"
                     /locus_tag="JNX27_003181"
                     /pseudo
     CDS             complement(12283..12699)
                     /gene="torD"
                     /locus_tag="JNX27_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
BASE COUNT         3049 a         3243 c         3212 g         3245 t
ORIGIN      
        1 ttcgctgagt gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta
       61 aacaggtagc cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct
      121 caataataat ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg
      181 tcgcgcctgt caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc
      241 aaatcataaa aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac
      301 cggcaatcag cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca
      361 ttaacaacgg cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat
      421 cgcgtgagac ttccagcttc agacggatgt actcttttat cgccgccagc ggggcgaaat
      481 cttcacgaaa cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa
      541 tatacagcgc ctcttttgac gggaagtaat acagcagatt ggtttttgaa acacccgcca
      601 actctgcgat ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg
      661 ctgcgctaag aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt
      721 tcactgcgcc ttgcgtcatg cgctctcccc tcttgtagat tggtaacagg atagcaaaac
      781 ccccgcccgg cctcgacctt ctgcactctc atcgcgcacg gatgcatgtt ttatgtgcaa
      841 ctgttttgac cgtttagtcc actttttacc agatatttta gataacacaa tgctaacaca
      901 ttaataacat tcacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct
      961 gaaaaggata tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg
     1021 ttgatgatga aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc
     1081 cacgcgccgc agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag
     1141 cattaccatt tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact
     1201 gagttctggg atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc
     1261 tcgcgcattc agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc
     1321 cgtatggccg caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact
     1381 ggctggcaaa agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc
     1441 cgtcgttacg actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg
     1501 aaaagcgatt ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg
     1561 agtatcccca tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc
     1621 cagtatgccg atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc
     1681 ccgaccgctg cgcggatgaa acaggccgca gagcaaaccg gacgcgacgt tggctcttat
     1741 gtgttgttta tggtgattgc cgatgaaacc gacgatgcca ctcgcgccaa atgggaacac
     1801 tacaaagcgg gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat
     1861 acccgctccg gtactgacac caacgttcgt cagatggccg atcccacttc ggcggtaaac
     1921 atcaatatgg ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc
     1981 gcaagcgtgc ctagtgcaga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc
     2041 gaaaccttcg gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ctctgcgctg
     2101 actcaggagg tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc
     2161 cgcagcaaag tgcgcagatc gtggtggata tgcaaaacgc ttatgccacg ccaggcgact
     2221 acttagatct cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa
     2281 ccgctgtgac cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg
     2341 atgaacagta tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc
     2401 tgaaaaccat gcgtaagcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg
     2461 attatcaact ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc
     2521 gctacagcgg tttcttcaat acgccgctgg acagcatttt gcgcagccgt ggaatacacc
     2581 atctggtttt caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct
     2641 tttttctgga gcatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat
     2701 ttgcacagaa agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg
     2761 aaacgttctg cgacgcgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac
     2821 gatgccaaaa tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc
     2881 cggcacgctg gctgatggct tggtgtatgt ctccggtacg ctggcttttg atcaacataa
     2941 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
     3001 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
     3061 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
     3121 taaaccggcg cgattctaca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
     3181 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
     3241 cttatgctga tgcacccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
     3301 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
     3361 gcaccggcaa taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg
     3421 aactgcatca ggcgctggta gccgcaggga ttgagcgtta cgcggtggtc ggccatgcgc
     3481 tcggtgcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta actatgctgg
     3541 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
     3601 aacgattact gtatagcggc ggcgcgcaag catgggtgga agcgcagccg ttgttcctct
     3661 atcccgccga ctggatggcg gtccgcgcac ctcgcctgga ggcagaagac gcgctggcac
     3721 tggcgcattt tcagggcaaa aataatttac tacgtcgact taacgccctc aaacgcgctg
     3781 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
     3841 atctgctggt gccaacagca tgttccagtg aacttcatgc cgccctgccc gatagccaga
     3901 aaatggtgat gccctatggc ggacacgcct gcaacgtgac cgatcccgaa acgtttaatg
     3961 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     4021 ttgctatgaa cgaagccgtt agcccaggtg tgcttagcac cctgttcacc gatgcccgca
     4081 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacaggag ctttatgccc
     4141 tgatgaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     4201 ctgcagaagg aaaagaacgt ctgcgcccgg cgctttccag cggcaatctg caaaaaaccc
     4261 tgaccgcgcc cgtcaccgct atcgtcgctt gggacagtga attttatgaa cggttaccac
     4321 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     4381 aaacagcgtt tcgcaacagt tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     4441 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     4501 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     4561 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     4621 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     4681 gcggtcaata tcatcaccac ggacggtcca gccgggcgcg ccgggttcac cgccagcgcc
     4741 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     4801 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     4861 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     4921 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     4981 tgccgtatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     5041 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     5101 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     5161 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     5221 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     5281 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     5341 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     5401 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     5461 cgaatatcag catcgcgctg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     5521 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     5581 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     5641 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     5701 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggatta attgtcgcct
     5761 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     5821 tcgtcagcta tgctgcctgg ttcggtctgc cgcatttttc cactcctggt tttaatggcc
     5881 aggcgatgat gttgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     5941 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     6001 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     6061 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     6121 cagctgccgt catcgctatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     6181 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     6241 caggcgcaag gatatgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     6301 tggtcgcagt aaccttcgtt ctgggcgcgg gtgattttgc tctcacgctt ggcggtttta
     6361 cgttgggagg gatcggtaca gcaacctttg gcgcgatttt actcaatgcg ttattaagca
     6421 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     6481 gccgggttac cggcggtgat gactttttga cgagcttaag aaaaaaatgc attcgtcatt
     6541 gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt tttacctgct
     6601 tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt
     6661 actgacctcc gtctgggtat agacatcata ttgcatagaa ttaacgcggc gtatggcggc
     6721 tgccatattt tcgctacgtg ggactaagct tagtgaactg acgcgggcag acccgcgttt
     6781 ggtaatatcc tgcaacagct tcttagcgag aatagatatc atctccaata attatccata
     6841 agccgctctt atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat
     6901 tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa
     6961 aggacgtctt atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg
     7021 agtggttaga aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa
     7081 cgatggcacg cgcagtcgct gagggtgcaa gcaaagtgga tggtgcagaa gttgtcgtta
     7141 agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa
     7201 ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac
     7261 ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc
     7321 tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg
     7381 gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat cacggcatgg
     7441 tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg
     7501 gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ctcacgccag ccaagccagg
     7561 aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta
     7621 acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt
     7681 cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt
     7741 cgccgggtac gacgaaaaaa tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt
     7801 agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac agaccatcga
     7861 acgtaaaaac gtttaaccag cgactccccc gcttctcgcg ggggagtttt ctgttatttc
     7921 accgcctcat tcaacacgct atcaaactta tccatcgggc agaaaccgtt ggcatctatc
     7981 gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg
     8041 gcattacgta actgttccgc actctggtac acatattcaa ttttcatcaa atcgcgattg
     8101 gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc
     8161 tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc tatgttggag
     8221 tcgtgcccaa ctaacaccgt gattttcgga gcactggcgc gatcggtgac cagggctttg
     8281 tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacaga
     8341 ctgtcctggt aaccgttttt cagcttcgac aataccttcc actgctggtc agatttgatt
     8401 tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt aaacgcatct
     8461 accagcgagt tgccgacttt cagcggcccg gaaacacctg gttcttgttg atacttggcg
     8521 ctaaaggtat ttttgccatc caccagcgaa cactgttgtt tctctttaca ggcaggggaa
     8581 tctttatagt taacgatttt ttccagtagc tggtagctgt cggtaagctg gagtttgctg
     8641 agctctttct ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc
     8701 atcgggttaa aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatca
     8761 cacccaggga atgcaccagt aataaagaac tgggcggtag cgacggtacg ttgcagacta
     8821 ttggcatagg cataaacggt gtccggcggc gggcattccc ccgatttcac tatcccctgc
     8881 tctgccagcc attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg
     8941 gtgagttgcc caccggggac gtcccattcg ggccatttat tcggcgtcga ctgctccagc
     9001 acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc
     9061 tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag taaaactatc
     9121 cctgccacag ttgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat
     9181 cattaaagag tgtgacagaa atatgacttt tttccgggaa caggttcgca aaatggcttg
     9241 ttgaacgcct gtttttctat atacatcttc aaaaacaggc aaggtttgac gcaaactatg
     9301 cctgattagc ccctgcctcg cgtacgccgc tgataattgc acctgtggct aaacagaata
     9361 tctgcggcgt taccaataca gagacaatat ttaccaataa aaaagtgtga gactaataac
     9421 aatggttttt agcatatgcc tttgccatta agttgcataa cgatttaaat agtcaagttg
     9481 cgcattcatt tccttatttt ttaacttata agcctgagaa aaaagttcag gagggaggta
     9541 aacatcataa tgattgccag tgtttattaa ataaccatac tcaagatctt catctgttga
     9601 aaaggattca tttcccaagg agattgatgc cacattacta ttaacaataa ataatgagca
     9661 aatgctatct gaaagaacag caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc
     9721 ggtatctaaa gcaagcaaat tcttgttatc attaaatata aactcgggta aatgcagtat
     9781 gcattttttg tcattcaagc aactatcaat gaattgtttt ttaaaaaaca gaaagctcca
     9841 tgacataaaa ctacccacac cctgaatttt gttgtcagat tgcagatcaa cgccaaataa
     9901 tgtctttaca tcttccctga gattatagaa tgccgtatca acaagagcct cgatcgtttg
     9961 tttatcaaca attttaataa actcttcgta tacttgcttt tgcgtcttat ggctgcccag
    10021 cgcccatgaa tactcatgtt cttttaaaac cagtatggct cttaacagac aatatccatc
    10081 gccatctact ggagcgtatt ttaatccata tgttttatta atttcactct gaataaaaga
    10141 tatgccagac aaatcaatac cagtatctaa atcagatatt ctcggcatat ttatctgttc
    10201 tggctggata ctcatatttt catcatctaa acaatactca gaaaaaacat catcacacgc
    10261 attaatgttt tcaatatatt ccataaagcc ataccaataa taacctttaa aatctgtgat
    10321 agactctgaa cctctgcccg gcaaggacac tctaacaata tcaacatctt gaacatattg
    10381 tatagtatat gtaatacctc ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa
    10441 tgttctcata aataatgata gatcattatc attctgcatt tcctcaaatt ctttttctag
    10501 tgattcacat tccagacatg gagtatcctc ttcatttact ttacgtctga gttcattgta
    10561 caaccgcata aaacacacca gtgtgttttc aaaaagcgtg attgaatatg tttgattttt
    10621 ttcgtactca ttttttatat cgttccaggc ctgtttcaaa taattatttg cagtgcaact
    10681 aattccatgt atattacgac ccatatatag cgtctataaa atttaataaa taatgacgcc
    10741 ctagttaaac ttaaagtgcc tggttcaact atcaaaaatc gctcacccgt tttcacctgt
    10801 ttaaaatatg ttcagcaacc catcttgatg gcgacctcct ctccgcgatg atttcaataa
    10861 catattctgt gttggcatat gaaattttga ggattaccct acacttatag gagttacctt
    10921 acaggggttc cttcaatttg tgttgattta cgcgagataa cgctatggaa ttaaaggatt
    10981 attacgccat catgggcgtg aaaccgacgg acgatctcaa gacaatcaag accgcctatc
    11041 gtcgactggc ccgtaaatac catcctgatg tcagcaaaga accggatgcc gaagcccgct
    11101 tcaaagaggt cgctgaagcc tgggaagtgt taagtgatga acaacgtcgc gctgagtatg
    11161 atcagatgtg gcaacatcgc aacgatccgc aatttaaccg tcagttccat catggcgacg
    11221 gtcagagttt taatgccgaa gattttgacg atatcttctc gtcaattttc ggtcagcatg
    11281 cccgccagag ccgtcaacgc cccgccgcac gcggccacga tattgaaatc gaagtggcgg
    11341 tattcttcga agaaacgctt actgagcata agcgtaccat cagctataac ctgccggttt
    11401 ataacgcctt tggcatgatc gaacaggaaa tcccgaaaac gctgaatgtg aagatcccgg
    11461 caggcgtcgg caatggtcaa cgcatccgcc tgaaaggcca ggggacgccg ggcgaaaacg
    11521 gcggtccaaa tggcgatttg tggctggtga ttcatattgc gccacatccg ctgttcgata
    11581 ttgtcggcca ggatctggaa attgtggtgc cggttagccc gtgggaagcg gcgctgggta
    11641 ctaaagtcac cgttccaaca ctgaaagaaa gcattttgct gactatcccg ccaggcagcc
    11701 aggccgggca acgattgcgc gttaaaggca aaggtctggt gagcaaaaaa cagaccggcg
    11761 atctgtatgc ggtactgaaa atcgtgatgc cgccgaaacc ggatgaaaac actgccgcac
    11821 tgtggcagca actggcagac gcccagtcgt cttttgatcc acgtaaagat tgggggaaag
    11881 cataatggct aatgttacgg tgacttttac tattaccgaa ttttgcctgc ataccggcat
    11941 ctctgaagag gagttgaatg aaattgtcgg tttgggggtg gttgaaccgc gtgagatcca
    12001 ggaaacaacc tgggtatttg acgaccatgc cgccattgtg gtgcaacgcg cggtacgcct
    12061 gcgtcatgaa ctggctctgg actggccggg gatcgcggtg gcgctgacgt taatggatga
    12121 tattgcgcac ctgaagcagg aaaaccgcct gctacgccag cggctttccc ggtttgtagc
    12181 tcatccgtga tgtgtgtttg ccggatgtga tgacgcatcc ggcaactgcg ccataatgcc
    12241 tgatgcgacg ctaaacgcgt cttttcaggc gcataaacga cgttatctgt tttggtggtc
    12301 gcactccact aacaccagca ataactggct tagtgccgcg taaaaaccaa agctgtcata
    12361 ctgtcgacaa cgcgcagcaa actctggtaa ccattgccgt agcgccgtca gtgttttttg
    12421 ccgcaaactg tcgattcttc gcgcaggaac ggtcccctct cccagcgaaa aatgcagata
    12481 actcaacaac tcgagataga tcgccagatg atctgccggt tcgttgaaat tgccactggt
    12541 ttccatccct gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga
    12601 tgcatacggc agcgccgctt gtttgtcggt catcagaaac aggccgcaaa agtcagcggc
    12661 cagttccaga cgggcatcgt cacgtactgt cagcgtggca ggtaatgctg ccaacttact
    12721 gatttagtgt atgatggtga ttttaaggt
//
LOCUS       DADLXX010000116        12294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLXX010000116 DADLXX010000000
VERSION     DADLXX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.117"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1231)
                     /locus_tag="JNX27_003182"
                     /pseudo
     CDS             complement(1..1231)
                     /locus_tag="JNX27_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1430..1514)
                     /locus_tag="JNX27_003183"
     tRNA            complement(1430..1514)
                     /locus_tag="JNX27_003183"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Leu,seq:caa)
     gene            1710..2729
                     /gene="ahr"
                     /locus_tag="JNX27_003184"
     CDS             1710..2729
                     /gene="ahr"
                     /locus_tag="JNX27_003184"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7884295.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2858..4372
                     /locus_tag="JNX27_003185"
     CDS             2858..4372
                     /locus_tag="JNX27_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7884296.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(4480..5562)
                     /gene="lptG"
                     /locus_tag="JNX27_003186"
     CDS             complement(4480..5562)
                     /gene="lptG"
                     /locus_tag="JNX27_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7884297.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKDDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5562..6662)
                     /gene="lptF"
                     /locus_tag="JNX27_003187"
     CDS             complement(5562..6662)
                     /gene="lptF"
                     /locus_tag="JNX27_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7884298.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6929..8440
                     /gene="pepA"
                     /locus_tag="JNX27_003188"
     CDS             6929..8440
                     /gene="pepA"
                     /locus_tag="JNX27_003188"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7884299.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8574..9017
                     /gene="holC"
                     /locus_tag="JNX27_003189"
     CDS             8574..9017
                     /gene="holC"
                     /locus_tag="JNX27_003189"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7884300.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9017..11872
                     /gene="valS"
                     /locus_tag="JNX27_003190"
     CDS             9017..11872
                     /gene="valS"
                     /locus_tag="JNX27_003190"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7884301.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11926..>12294)
                     /locus_tag="JNX27_003191"
     CDS             complement(11926..>12294)
                     /locus_tag="JNX27_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7884302.1"
                     /translation="RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRS
                     TLTYHGMLYRMCALVVISEITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQ
                     PDKGFLASISRGVMPSLPFL"
BASE COUNT         3030 a         3218 c         3305 g         2741 t
ORIGIN      
        1 tgaggtgagc atatcacctc tgttcaggtg gccaaattca gtgtgccact tcaaacatca
       61 acctacgctc cccaagatgt tccgccttat ggatgtaagc tgctcgcaca gagctacgct
      121 cctggtgact catctgccgc tctaccgcat ccctcgacca taatcccgac tcaatcaacg
      181 aactacaagc catcgtcctg aagccatgcc cacaaacctc gaccttcgtg tcatacccca
      241 ttacgcgcaa agccttgttc accgtattct cactcatcgg cttacgtgga tcgtgatcgc
      301 ccacaaaaat cagctttcga tttccgctca tgcttttgat cttttccaaa atacttaggg
      361 cctgccgcga caaaggtaca aggtgtggtg tccgcatctt cgagccacgc tgagaatgct
      421 tgactccttc cagttgctca cgctcacctg ggattgtcca catagccgtt tcaaaatcta
      481 cttctgacca gcgggcaaaa cgcagctcgc tcgaacggat gaaaaccaat aaagtgagtt
      541 caaccgccag tcgagttaac ggtctgccag aatagtgatc tatgcgatga agtaattcag
      601 gtatacggtt cagttcaaga gctgcacggt gctgtctttt cgccgtagca accgcacctg
      661 caatctcttg cgcggggttg tagtcgatta agccgctctg aacggcaaag cgcataatcg
      721 cggtagtacg ctgctgtaaa cgggcggcaa cttcgagccg cccggatgac tcgactgctt
      781 taatgggtac aagaagatcc cgcgtcttca gttccgcaat gttccgctta ccgatagcag
      841 taaagagatt atcctccaga cttttcaaaa cacgagcgct atgcgatgcc gaccacttct
      901 gattgctggc gtgccagtct ctggcgacta cttcaaacgt tatcgcctct tgttcctgtt
      961 ctaccttaac agctttcttg ttttcactgg gatcgacacc attagctaaa agcttacgag
     1021 cctcatcccg gcgtgccctg gcatccgcca acgacacttc ggggtatttc cccaatgcaa
     1081 gcatcttctc tttgccgcca aagcgataac gtagccgcca gtatttggag ccgttagggt
     1141 gaaccagcaa aaccatgcct tcaccgtcgg tcagcttata ggcttttgct tcaggcttag
     1201 ccgaacgaac cttcacatca ctcagagcca tgatgagtat cctttcaagg gttctgtgtg
     1261 ggtacaaaca ttatcgaacc gggaaatacc cgcagttgta cccgcatcag taagttgatg
     1321 tagattgaat caggttgact taggttgagt gaaaaagcga ggaaagcctt gcggatactg
     1381 gattttaggc acaaaaaaag acgtccgttg acgtctattg atgttccgat ggtgccgaag
     1441 gccggactcg aaccggcacg tatttctacg gttgattttg aatcaactgc gtctaccgat
     1501 ttcgccactt cggcactgaa gaggtatgcg gaaaacgttg tggattatac ctgtcgcacg
     1561 ccaccatgca agcgccagct tgcacccacc tcgctaagtg ctgaaatttt cagcattaca
     1621 ctcagcgata tgtaaccttt gtcacactcc aggccccccg ccctgccatg ctctacactt
     1681 cccaaacaac accagagaag gacccaaaaa tgtcgatgat aaaaagctat gccgcaaaag
     1741 aagcgggcgg cgaactggaa gtttatgagt acgatcccgg tgagctgaag ccacaagatg
     1801 ttgaagtgca ggtggattac tgcgggattt gtcattccga tctgtcgatg atcgataacg
     1861 aatggggatt ttcacaatat ccgctggttg ccgggcatga ggtgattggg cgcgtggtgg
     1921 cactcgggag cgccgcgcag gataaaggtt tgcaggtcgg tcagcgtgtc gggattggct
     1981 ggacggcgcg tagctgtggt cactgcgacg cctgtattag cggtaatcag atcaactgcg
     2041 agcaaggtgc ggtgccgaca attatgaatc gcggtggctt tgccgagaag ttgcgtgcgg
     2101 actggcaatg ggtgattcca ctgccagaaa atattgatat cgagtccgcc gggccgctgt
     2161 tgtgcggcgg tatcacggtc tttaaaccac tgttgatgca ccatatcact gctaccagcc
     2221 gcgttggggt aattggtatt ggcgggctgg ggcatatcgc tataaaactt ctgcacgcaa
     2281 tgggatgcga ggtgacggcc tttagttcta atccggcgaa agagcaggaa gtactggcga
     2341 tgggtgccga taaagtggtg aatagccgcg atccgcaggc actgaaagcc ctgtcggggc
     2401 agtttgatct cattatcaat actgtgaacg tcagcctcga ctggcagcct tattttgagg
     2461 cgctgaccta tggcggtaat ttccatacgg tcggtgcggt tctcacgccg ctgtctgttc
     2521 cggcctttac gttaattgcg ggcgaccgca gcatctctgg ttctgctacc ggcacgcctt
     2581 atgagctgcg aaagctgatg cgctttgccg cccgcagcaa ggttgcgccg acaaccgaac
     2641 tgttcccgat gtcgaaaatt aacgacgcca ttcagcatgt acgcgatggt aaagcgcgct
     2701 accgagtagt cctgaaagcc gacttctgat ccctttgccg ggtgctgtcg cccggcactt
     2761 aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag cggtgaagag tgcacagccg
     2821 ttgcctatac tccaggcagg aaactggagg aaatctcatg agcgaacccc tgttaattgc
     2881 ccgcacgccg gacacagaac tgtttttact gccgggaatg gctaaccgtc acgggctgat
     2941 tactggcgca acggggacgg gtaaaaccgt tacgctgcaa aaactggcag agtcattgtc
     3001 ggaaatcggc gtgccggtgt ttatggctga tgtgaaaggc gatctgaccg gtatcgcgca
     3061 ggcaggaacg gcgtcggaaa aactgctcac aaggcttaaa aatatcggcg tcaatgactg
     3121 gcaaccgcat gccaatccgg tggtggtgtg ggatatcttt ggcgagaaag gccacacggt
     3181 gcgggcgacg gtttcggatc tggggccgct gttgctggcg cgactgttga atctcaacga
     3241 tgtgcaatct ggcgtgctga atattatctt ccgcattgcg gacgatcagg gattgttgct
     3301 gctcgacttt aaagatttgc gggcgattac ccagtacatc ggcgataacg ccaaatcctt
     3361 ccagaatcag tacggaaata tcagtagcgc atcggttggt gccatccagc gcggattact
     3421 gtcgctggaa cagcaaggcg cagcacactt ctttggtgag ccgatgctgg atatcaaaga
     3481 ctggatgcgc accgatgcca acggtaaagg cgttatcaat atcctcagcg ccgagaaact
     3541 ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctttcag agttgtatga
     3601 acaattgccg gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaggc
     3661 acatctgctg tttaacgatg caccgcaggt actgctggat aagattgagc aggtgataag
     3721 gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt ctgatattcc
     3781 ggataatgtg ctcggccagc tcggtaatcg tgttcagcac gctttgcggg cttttacgcc
     3841 aaaagatcag aaagcagtga agacagcggc gcaaaccatg cgggccaatc cggcatttga
     3901 taccgaaaag gcaatccagg aactggggac cggcgaggcg ttaatctcgt ttctcgatgc
     3961 aaaaggaagt ccttctgtgg tggaacgggc gatggtgatc gcaccttgtt cgcgaatggg
     4021 gccggtgacg gaagatgagc gtaatggcct gattaatcac tccccggtgt atggcaaata
     4081 tgaagatgag gtggaccgcg agtccgccta tgagatgctg caaaaaggct ttcaggccag
     4141 taccgagcag caaaataatc cccccgtgaa agggaaagag gtggcggtgg atgacggtat
     4201 tcttggtgga ttgaaggata ttttgtttgg cactaccgga ccacgcggcg ggaagaaaga
     4261 tggtgtggtg caaacaatgg cgaaaagcgc cgctcgccag gtgacgaatc agattgtacg
     4321 cgggatgttg gggagtttgc tgggggggga gaaaaagata agtcagacgt gatctgtgcc
     4381 cctcacccta accctctccc caaaggggcg aggggaccgt tcgaactcgt atttgacttt
     4441 gtcaacaata ataaagggag ctttcgctcc cttgttagat tacgattttc tcattaacag
     4501 ccacaggctg attaagaaga agctggcact tggcaacagt gcgccgatga tcggcgggat
     4561 gccgtaaacc aacgtcagcg ggccgaagat ctggtccagt acgtagaaga caaaaccgaa
     4621 gctgatacct gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagataaa
     4681 cgacagcgcc atcagcatca tcaccgccac ggatagcggc tggaagattt tgctccacat
     4741 gttgagctga taacgtccgg catcctgacc gctcgacttc agatacttca cgtagttgtg
     4801 caaaccactg atggagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt
     4861 gaggtcggtt ttccaggtgc cgctcaccgt ctgcgaaccg gtaatctgtt tcggattggt
     4921 cagatcagat tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc
     4981 ggtagcggca taacgtacgg attgcagacg acgattctcg ttaaaggcat aaatactgat
     5041 gccacctaac acctcgtcat ctttaacccg ctcaatgtag acgaagttgt tgccatcttt
     5101 cgcccataaa ccttgctggg tagagagcaa cgagccgccg tacatcgcct gcgcacggta
     5161 gttacgcgcc atctgttcgc cctgcggcgc gacccattcg ccaatcgcca tcgtcagcaa
     5221 gaccagtgga atggcggttt tcatcaccga cagcgctacc tgcatacggg taaaaccaga
     5281 agcctgcatc accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc
     5341 aagaagagcc gccatcggga agaagatctg cacatctttc ggcacgctca gcaaggtata
     5401 cattcctgcg cctaacgcgt cgtaactccc ctgcccggct tttttcagct gatcgacaaa
     5461 tttgataatg cccgacagcg acaccagcat gaacagcgtc atcatgatgg tggtgaaaat
     5521 agttttaccg atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc
     5581 gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc
     5641 aaagccagat aaatcaggtt aacggtccac atccacagtg tcgggtccag cttaccttta
     5701 ccgccgttcg atttcaggga ggtctggatc aggaagaaaa gtagatacag cagcatggct
     5761 ggcagcatcg acagtacgcg tccctgacgc gggttaacca cgctcaacgg tacaaccata
     5821 agtgccatca taaacacggt gaataccaac gtgatacgcc agttcagttc tgcgcgagca
     5881 cgatcggtgt cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg
     5941 agcgccaccg cctggtgacc aatgatcgcc tgataatcct ggaaatccgt aatgcggaaa
     6001 tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag
     6061 ccgtcgcgca gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta
     6121 ccttttggtc gaatttgtgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg
     6181 atgaacagca ccgagctgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc
     6241 atgccagggt tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt
     6301 cccgcccaca tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc
     6361 gctttcacca gaaccgcttt gctcaagccg caggcatgca ttaccgtaat ttcactttcg
     6421 gtatacagtt tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc
     6481 tgcgccattt ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg
     6541 ccgtcaaccg ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag
     6601 atgaagagta tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc
     6661 actttaaata cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta
     6721 aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat
     6781 tcacctctca gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa
     6841 gtcatcgcaa cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg
     6901 ttgtctttaa gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga
     6961 gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat
     7021 tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gccggggcga
     7081 actggaagga aaaccggggc agacattgtt gctgcaccat gttccgaatg tactttccga
     7141 gcgaattctc cttattggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca
     7201 ggttattcag aaaaccatta atacgctgaa tgatactggc tcgatggaag cggtctgctt
     7261 tctgactgaa ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga
     7321 gacagcaaaa gagacgctct acagtttcga tcagttgaaa acgaataaga gcgaaccgcg
     7381 tcgtccgctg cgtaaaatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga
     7441 gcgcgcgatc cagcacggtc tggcgattgc cgccgggata aaagcagcga aagatctcgg
     7501 taatatgccg ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc gccagctggc
     7561 tgacagctac agcaagaatg tcatcacccg cgttatcggc gaacagcaga tgaaagagct
     7621 gggaatgcac tcttatctgg cggtcggtca gggttcacaa aacgaatcgc tgatgtcggt
     7681 gattgagtac aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg
     7741 tttaaccttc gactccggcg gtatctccat caagccttca gaaggcatgg atgagatgaa
     7801 gtacgatatg tgcggcgcgg cggcggttta cggcgtgatg cgtatggtcg cggagctgca
     7861 actgccgatt aacgttattg gcgtgctggc aggctgtgaa aacatgcctg gcgggcgtgc
     7921 ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa accgttgaag tgctgaacac
     7981 cgatgccgaa ggccgcctgg tactgtgcga cgtgttaact tacgttgaac gttttgagcc
     8041 ggaagcagtg attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca
     8101 tattactggt ctgatggcga accataatcc gctggcccat gaactgattg ccgcgtctga
     8161 acaatccggt gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacagctgga
     8221 gtccaatttt gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg
     8281 ttgcttcctg tcacgcttta cccgtaagta taactgggcg cacctggata ttgcaggaac
     8341 cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt tgctggcaca
     8401 gttcctgtta aaccgcgctg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc
     8461 ctaaccctct ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc
     8521 tttcagggtg aggaatgcag caatatttaa atccaccaca agaagcccca tatatgaaaa
     8581 acgcaacgtt ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc
     8641 aactggtgtg tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgta ctcatcgcct
     8701 gtgaagatga aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa
     8761 gctttgttcc gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg
     8821 cctggccgca aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct
     8881 ttgcagattt tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc
     8941 tgaaacaact ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata
     9001 cggcaacctg gaaataatgg aaaagacata caacccacaa gatatcgaac agccgcttta
     9061 cgagcactgg gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt
     9121 ctgcatcatg atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca
     9181 gcaaaccatc atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg
     9241 gcaggtcggt actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc
     9301 cgcagaagaa ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg
     9361 ggaatggaaa gcggaatctg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc
     9421 cgtcgactgg gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga
     9481 agttttcgtt cgtctgtata aagaagacct gatttaccgt ggcaagcgcc tggtaaactg
     9541 ggacccgaaa ctgcgtaccg ctatttctga cctggaagtg gaaaaccgcg aatcgaaagg
     9601 ttcgatgtgg cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga
     9661 ttatctggtg gttgcgacca ctcgcccgga aaccctgctg ggcgataccg gtgtagctgt
     9721 taacccggaa gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt
     9781 taaccgtcgt attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg
     9841 cgtgaagatc accccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct
     9901 gccgatgatc aacatcctga cctttgacgg tgatatccgt gaaagcgccc aggtgttcga
     9961 taccaaaggt aacgaatctg acgtttattc cagcgagatc ccggcagagt tccagaaact
    10021 cgagcgtttt gctgcacgta aagcagtcgt tgctgcggtt gatgcactcg gcctgctgga
    10081 agaaattaaa ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tggttatcga
    10141 accgatgctg accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcagttga
    10201 agcggttgag aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc
    10261 ctggatgcgc gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat
    10321 cccggcatgg tatgacgaag cgggtaatgt ttatgttggc cgcaacgaag aagaagtgcg
    10381 caaggaaaat aacctcggtg ccaacgttgc cctgcgtcag gacgaagacg ttctcgacac
    10441 ctggttctct tctgcactgt ggaccttctc tacccttggc tggccggaaa ataccgacgc
    10501 cctgcgtcag ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg
    10561 gattgcccgc atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca
    10621 ggtgccgttc cacactgttt acatgaccgg tctgattcgt gacgacgaag gtcagaagat
    10681 gtccaaatcc aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc
    10741 agaactgctg gaaaaacgca ccggcaatat gatgcagccg cagctggcgg acaaaatccg
    10801 taagcgcacc gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg
    10861 cttcaccctg gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgtct
    10921 ggaaggctac cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa
    10981 cacagaaggt caggattgcg gcttcaacgg cggcgaaatg acgctatcgc tggcggaccg
    11041 ctggattctg gcggagttca accagactat caaagcgtac cgcgaagcgc tggacagctt
    11101 ccgcttcgat atcgccgcag gcattttgta tgagttcacc tggaaccagt tctgcgactg
    11161 gtatctggag ctgaccaagc cggtaatgaa cggtggaaca gaagccgaac tgcgcggtac
    11221 tcgccatacg ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc
    11281 gttcatcacc gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac
    11341 catcatgctg cagccgttcc cgcagtatga tgcatctcag gttgatgaag ccgcactggc
    11401 cgacaccgag tggctgaaac aggcgatcgt tgctgtacgt aacatccgtg cagaaatgaa
    11461 catcgcgccg ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg
    11521 tcgcgtaaat gaaaaccgtg gcttcctgca aacgctggcg cgtctggaaa gcatcaccgt
    11581 gctgcctgcc gatgacaaag gtccggtttc cgttaccaag atcatcgacg gcgcagagct
    11641 gctgatcccg atggctggcc tcatcaacaa agaagatgag ctggcgcgcc tggcgaaaga
    11701 agtggcgaag atcgaaggtg aaatcagccg catcgagaac aagctggcaa atgaaggctt
    11761 tgtcgcccgc gcaccggaag cggttatcgc gaaagagcgt gagaagctgg aaggttatgc
    11821 agaagcgaaa gccaagctga ttgaacagca ggctgttatc gccgcactgt aatcaacaca
    11881 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    11941 agaaggcata actccccgag aaatactggc taagaagcct ttatctggtt gttcttcttt
    12001 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    12061 aatcatccat attttcagca gtgggtaagc cagaccaccc gtaatctcgg atatcaccac
    12121 caacgcacac atgcgataaa gcataccgtg gtacgttaaa gttgagcgaa aacgaatacg
    12181 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    12241 ggtgctaaca gcaatcccaa aataataaat taactgcgcg attatcattt tacg
//
LOCUS       DADLXX010000117        12230 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLXX010000117 DADLXX010000000
VERSION     DADLXX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.118"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..494)
                     /locus_tag="JNX27_003192"
     CDS             complement(<1..494)
                     /locus_tag="JNX27_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884303.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JNX27_003193"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JNX27_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7884304.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JNX27_003194"
     CDS             complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JNX27_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY7884305.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2441..3307
                     /gene="folD"
                     /locus_tag="JNX27_003195"
     CDS             2441..3307
                     /gene="folD"
                     /locus_tag="JNX27_003195"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7884306.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3309..3521
                     /gene="ybcJ"
                     /locus_tag="JNX27_003196"
     CDS             3309..3521
                     /gene="ybcJ"
                     /locus_tag="JNX27_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7884307.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3629..4150
                     /locus_tag="JNX27_003197"
     CDS             3629..4150
                     /locus_tag="JNX27_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7884308.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JNX27_003198"
     CDS             complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JNX27_003198"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7884309.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5745..6239
                     /gene="ppiB"
                     /locus_tag="JNX27_003199"
     CDS             5745..6239
                     /gene="ppiB"
                     /locus_tag="JNX27_003199"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7884310.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6242..6964
                     /gene="lpxH"
                     /locus_tag="JNX27_003200"
     CDS             6242..6964
                     /gene="lpxH"
                     /locus_tag="JNX27_003200"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7884311.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7082..7591
                     /gene="purE"
                     /locus_tag="JNX27_003201"
     CDS             7082..7591
                     /gene="purE"
                     /locus_tag="JNX27_003201"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7884312.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7588..8655
                     /gene="purK"
                     /locus_tag="JNX27_003202"
     CDS             7588..8655
                     /gene="purK"
                     /locus_tag="JNX27_003202"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7884313.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8792..9685)
                     /locus_tag="JNX27_003203"
     CDS             complement(8792..9685)
                     /locus_tag="JNX27_003203"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7884314.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9682..10329)
                     /locus_tag="JNX27_003204"
                     /pseudo
     CDS             complement(9682..10329)
                     /locus_tag="JNX27_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10332..11564
                     /gene="allC"
                     /locus_tag="JNX27_003205"
     CDS             10332..11564
                     /gene="allC"
                     /locus_tag="JNX27_003205"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY7884315.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11575..>12230
                     /gene="allE"
                     /locus_tag="JNX27_003206"
     CDS             11575..>12230
                     /gene="allE"
                     /locus_tag="JNX27_003206"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY7884316.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     "
BASE COUNT         2965 a         3168 c         3120 g         2977 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt
     1261 ggcttcttac aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac
     1321 gtggtttcga tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat
     1381 aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag
     1441 tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag
     1501 aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat
     1561 caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg
     1621 ttgtatttgt actggatgca atggccaata aattatcatt gattgcatca gcctgaccag
     1681 aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga
     1741 atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga
     1801 gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc
     1861 cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata
     1921 gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat
     1981 ttatcgactc cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa
     2041 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     2101 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     2161 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2221 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa
     2281 tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct
     2341 tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc
     2401 atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga
     2461 cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat
     2521 tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc
     2581 gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc
     2641 ttatgacctc ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa
     2701 tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa
     2761 cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa
     2821 cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt
     2881 cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc
     2941 atcgaatatc gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac
     3001 cgtgactcac cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat
     3061 cgttgccgtt ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt
     3121 gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga
     3181 agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac
     3241 ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg
     3301 tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc
     3361 tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc
     3421 aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga
     3481 cagtgagttt tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat
     3541 caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata
     3601 gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc
     3661 cctttgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg
     3721 aatcatccta gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     3781 cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc
     3841 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     3901 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     3961 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     4021 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     4081 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     4141 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     4201 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     4261 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     4321 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     4381 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     4441 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     4501 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     4561 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     4621 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     4681 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     4741 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     4801 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     4861 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     4921 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     4981 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg
     5041 gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg
     5101 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc
     5161 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     5221 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     5281 gtgcatttcg gcgatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     5341 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     5401 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     5461 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     5521 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     5581 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     5641 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     5701 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     5761 ccaatcacgg cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa
     5821 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     5881 acggctttat gattcagggc ggcggttttg aaccgggcat gaaacaaaaa gccaccaaag
     5941 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     6001 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     6061 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     6121 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     6181 tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     6241 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     6301 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     6361 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     6421 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     6481 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     6541 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     6601 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     6661 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     6721 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     6781 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     6841 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     6901 tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt
     6961 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     7021 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     7081 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     7141 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     7201 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     7261 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     7321 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     7381 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     7441 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     7501 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     7561 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     7621 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     7681 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     7741 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     7801 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     7861 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     7921 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     7981 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     8041 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     8101 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     8161 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     8221 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     8281 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     8341 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     8401 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     8461 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     8521 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     8581 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     8641 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     8701 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     8761 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     8821 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     8881 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     8941 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     9001 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     9061 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     9121 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     9181 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccgg cgcaaatatt
     9241 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     9301 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     9361 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     9421 ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt
     9481 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     9541 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     9601 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     9661 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     9721 ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     9781 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     9841 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     9901 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     9961 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
    10021 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
    10081 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
    10141 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
    10201 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
    10261 ttgagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
    10321 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
    10381 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
    10441 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
    10501 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
    10561 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
    10621 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
    10681 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
    10741 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
    10801 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa
    10861 ctccgcgtca ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
    10921 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
    10981 tgacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
    11041 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
    11101 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
    11161 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
    11221 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
    11281 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
    11341 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
    11401 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
    11461 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
    11521 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
    11581 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
    11641 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
    11701 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
    11761 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
    11821 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
    11881 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
    11941 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat
    12001 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    12061 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    12121 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    12181 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg
//
LOCUS       DADLXX010000118        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLXX010000118 DADLXX010000000
VERSION     DADLXX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.119"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            56..1327
                     /locus_tag="JNX27_003207"
                     /pseudo
     CDS             56..1327
                     /locus_tag="JNX27_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1340..2110
                     /locus_tag="JNX27_003208"
     CDS             1340..2110
                     /locus_tag="JNX27_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY7884317.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2163..2558
                     /locus_tag="JNX27_003209"
     CDS             2163..2558
                     /locus_tag="JNX27_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884318.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2599..3342
                     /gene="cmoA"
                     /locus_tag="JNX27_003210"
     CDS             2599..3342
                     /gene="cmoA"
                     /locus_tag="JNX27_003210"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7884319.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3339..4310
                     /gene="cmoB"
                     /locus_tag="JNX27_003211"
     CDS             3339..4310
                     /gene="cmoB"
                     /locus_tag="JNX27_003211"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7884320.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4475..6904)
                     /locus_tag="JNX27_003212"
     CDS             complement(4475..6904)
                     /locus_tag="JNX27_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7884321.1"
                     /translation="MALTIREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAVMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6929..8029)
                     /locus_tag="JNX27_003213"
     CDS             complement(6929..8029)
                     /locus_tag="JNX27_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7884322.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNX27_003214"
     CDS             complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNX27_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7884323.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9177..9743)
                     /locus_tag="JNX27_003215"
     CDS             complement(9177..9743)
                     /locus_tag="JNX27_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7884324.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9959..11692
                     /gene="argS"
                     /locus_tag="JNX27_003216"
     CDS             9959..11692
                     /gene="argS"
                     /locus_tag="JNX27_003216"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7884325.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRSLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11869..>12153
                     /locus_tag="JNX27_003217"
     CDS             11869..>12153
                     /locus_tag="JNX27_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAY7884326.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNNHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         3031 a         3066 c         2938 g         3118 t
ORIGIN      
        1 tttaatatca tgaaatatgt aagctcgtat cgctcttggg cgtctggagg tatcgatgga
       61 taaagtcaaa tatccaacag gcgtcgaaaa ccacggcggc acattacgca tctggtttaa
      121 ttttaaaggt aaacgtgtca gggaaaatct tggtgtccct gacactgcca agaacaggaa
      181 gatcgccggg gaactgcgga catcggtatg ttttgccatc cgcacaggaa gctttgatta
      241 tgctgcacag ttccctgact cccctaacct tcaggctttt ggggtaagta aaaaagaaat
      301 tacggtgaag gaacttgaag aaaagtggct ggatctgaaa cgaatggaaa tctctgcaaa
      361 tgcattcaat cgctatgaat ccgttgcaag aacgatggtt ccgaaaattg gaggcggcag
      421 actggtgtca tcggtaacca aagaggaatt gctgtatatc aggaaagatt tgctgaccgg
      481 gtatcagaat tcaacgaaaa acaaagcagc agcaaaagga cggagcgtcg ttactgtaaa
      541 ttattacatg acgacaatcg ctggaatgtt tcagtttgct gcagatcacg gttacttaga
      601 agcaaatccc ttccagggaa ttaagcctct taaaagagcc agggcagagc cagatccgtt
      661 aactcgtgac gaatttattc gcctgataga tgcttgccga catcagcaga cgaaaaacct
      721 gtggtcattg gctgtgtaca caggaatgcg tcacggtgaa ctggtctccc tggcctggga
      781 agatatcgat atgaaagcag gaactattac tatcaggcgc aattatacga aactcggtga
      841 gttcactcta cctaaaactg aagcaagtac aaacagggtt gtgcatctta tccagcccgc
      901 tatcagtgtc ctgaaaaatc aggctgaaat gacaagactg ggtaagcagc accacatcaa
      961 ggttcaacta cgtgaatatg gacgttcagt gaatcatgaa tgtactttcg tatttaaccc
     1021 ccaggtggtt agaaaaagca aacaggtagg ttttatctac aaggtagatt ccattggcga
     1081 ctcatgggaa acagccatta agcgtgcggg catcaggcac cggaaagcat accagtcacg
     1141 acacacttat gcgtgctggt cattatctgc cggagcaaac ccaagcttca ttgccagcta
     1201 gatgggccat gcaagtgccc agatggtgtt caatgtatac ggagcatgga tgactgacag
     1261 taatgcagaa cagatcgcaa tgctgaatca gaagctggca gattatgtcc catggtcacc
     1321 aaagtgacac aagagactta ttaaaatcag tgggttagcc ttcaataccc gtcatgttaa
     1381 ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa cccgaggttg tcctgcgctg
     1441 gcgtgaacag accacagatg acttccgctt ctgttttaag tttccggcga ccatttcgca
     1501 tcaggcagca ttacgacatt gcgatgattt agtgactgaa tttttgaccc gcatgtcacc
     1561 gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg gcccacggga
     1621 gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt atggtgtgga
     1681 agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta atcgcggttt
     1741 acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg catcacgtcc
     1801 acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
     1861 actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga cgcaaaaccg
     1921 ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca ctacgcctta
     1981 tcttttttta catacgccag atattgccca ggccccggaa ctggtacata ccctgtggga
     2041 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
     2101 tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt
     2161 gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
     2221 atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt tttagcgaac
     2281 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgc
     2341 tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
     2401 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg tttcgctggc
     2461 gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
     2521 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
     2581 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
     2641 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
     2701 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
     2761 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg
     2821 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc cccggcgatg
     2881 attgaacgct gccgtcgtca tattaacgcc tataaagccc ctacgccagt agacgtcatt
     2941 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
     3001 ctgcaattcc tggaaccttc cgagcgccag gcattactgg ataaaattta tcaaggactg
     3061 aacccgggca gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
     3121 gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
     3181 atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat
     3241 aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac
     3301 tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
     3361 attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc gcgcagattg
     3421 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac acggtggaat
     3481 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg
     3541 aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg aacctgatgc
     3601 catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
     3661 actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc accattctgg
     3721 atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg
     3781 cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
     3841 tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt ccggcactga
     3901 aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg ccgctggagc
     3961 atctctggca gttgaaagat caactggtga atgagggtga actggtgctg gaaacgctgg
     4021 ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg caaatgcgta
     4081 atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
     4141 tagatatccg cattgtggat gtgtgcgtta ccactactga agagcagcga cgcactgaat
     4201 ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg
     4261 aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa aggtctggta
     4321 ttactgccgg atgcggcgtg aacgccttat ccggcctaca aacccttgct aattcaatct
     4381 attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagtttta cgtttgcata
     4441 acctcagcgc ccgtttccgg gcgctattca cttcttacgc ctgaacagca ggcttatcaa
     4501 atgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc gcagagttac
     4561 cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc
     4621 cgtttttaca caagccgttt tccagatctg gccatgcacc ttcatgcacg cataccacgc
     4681 cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat ctgaccacgt ttgttccaga
     4741 cacgcaccag atcgccattc gcaataccaa atcgagcagc atcttcggtg tgaatagtga
     4801 tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt
     4861 gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct
     4921 tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt
     4981 ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca tttttcaccg
     5041 gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt
     5101 cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg cgttgcgcac
     5161 gggccccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca tctttacctt
     5221 cggtatagat ctcttttccg ccaggtttga gtaattccgc aagatcggca aacacgtcaa
     5281 aatcgttacg cgcttcaaat tgcggagcaa cagcctgctt catcggcaca atatgctggt
     5341 tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata
     5401 cgatatctgc gtgtttggct gctgcggtcc agtagcattc agaaacgacg atcatctccg
     5461 gtttctgcca ggctttgatc agacgattag tatcctggtg atgggtaaag ttaccgccac
     5521 ccgcccacca gatcattttg atattcggat acgtttgttc tttaccgtta tgctgatatt
     5581 tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc gtcattccgc
     5641 catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc
     5701 cgccaacacg catcgggttg ccaccattgg agtaatgata agagaaacca aaaccgccgc
     5761 ccggtgtacc aatctgccct aacatcaccg ccagcgtcac cagcatccag tgtttctgtt
     5821 caccatattg ctggcgctga attccccagc ctgccatcag aattgtgcga ttcgccgcca
     5881 tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc gccgcccaga
     5941 ccgcgctctt cggcgtatta tcgctcttac ctgtcagata ctcttcaaac tgcggatacc
     6001 cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg
     6061 caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat
     6121 cgccaaagaa ttcgatggtt tcggagcgga taggatcaat ggcaatcact ggtttgccag
     6181 attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt
     6241 ttaaggtatt cagcgggttc attccccaca gcaccacaac ctggctgttt tccagaatca
     6301 gcggccagga ggcctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca
     6361 cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca
     6421 tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc cagccgtaag
     6481 atccggcaaa aatggccgat ggtccgttag ctttacgaat acgctcatgt tgctcatgaa
     6541 tcagttttaa tgcctgttcc cagctcacct gtacataggt atcttcgcca cgacctttcg
     6601 ccggttgcag tggattgtcg agatagcttt ttctcaccat cggatgctga atacgcgccg
     6661 tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt cggtatggtt ttcgccagcg
     6721 cgcctgtcga agaaacaatc ttgccgtcct tcacttctac gctcatcgct ccccaacgtc
     6781 ccgcggtgag gattttaccg cccttctctt ctgcccatgc ggggagcggt gctgccgatg
     6841 tcaccaccag cgctccagcg gcaataccgc tgtgtttaat aaattcacgt attgttaatg
     6901 ccataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt
     6961 actggaaata ccgcgttaaa atgtccagtt cgttttcgct catgctggtt cgtgccccca
     7021 ttcctttggc aatggacggc cacgcattga cggtgtaatg gtcggcggca atagaggcat
     7081 gacaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc ggtttacggt
     7141 ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat tgattgccgt
     7201 attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct tcacttaatg
     7261 tggctagcgc cagccgttga cctgccgcca ggtagagcgt gttttcactg ccctgcattt
     7321 gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg gtgagatcgg
     7381 taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga taaatatgtg
     7441 cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca
     7501 tttttatttc tggcggaaaa tgggcaatgc ctttatgaca atcgatacag gtttcgccgc
     7561 ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca atatccatgg
     7621 catcaaaaga atggcaacta cggcacgttg cagagtcagt tgcttttaat tctttccata
     7681 ctgtttcggc catttcctgg cgatgagttt cgaacttatc gtcactgtct attttgccgc
     7741 taacaaattc atgataaata tctttagatg cttttaattt agcaaataaa taatccatcc
     7801 ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg ttcgaaaagt
     7861 ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg
     7921 ctgtatccga cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca
     7981 caaccgctat cagcaaaaac aataacccaa tgcgtttttt ccctcgcata gattcctcta
     8041 aataaattcc agaacaccga aatcattgta taattaaata cacaacgaat taaccgcttg
     8101 cataactcgg cacaacactg cctgaaataa tcgataaaga atatgattta ttacaatgta
     8161 atcattaatt gctaaggaat aacccagttg ctatttagaa tatatattta tcagttttag
     8221 caatttaaat cccataatca atgtgaatat aaaaaatatt tttagcacaa cgctatgcgc
     8281 actgtcaccc gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa
     8341 aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat
     8401 gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa
     8461 ccgccgcccc gtctacgata taacgcgaat actcgtccgc gtgttcatct gatgacatgg
     8521 acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga
     8581 cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact cctgctccag
     8641 ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca
     8701 gtgcgtctga tttttgccct gatgtcagta cccgggcaat gcccaattcc gcaagattat
     8761 tgagtgtata taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg
     8821 gaccggcagc agccattatt ttttccattc gtggcatatc gacattccca tcaacatcga
     8881 gaacgcccgt caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa
     8941 tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat
     9001 gcacagggat cgtcacccgc tggcgcacgg atttcagtcc ccccaacgac ggcgttaagc
     9061 ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta
     9121 gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttaattac
     9181 gctgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt
     9241 ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggtgaa
     9301 ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa tgccagcgca
     9361 cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg
     9421 tgtgggtaac gtttttcccc aggccacggt agctccacat tggaaaactg ccagtgcgca
     9481 acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat cattttttct
     9541 gacaaaagct cgccacactg ttcaaaaccg cgacgccagc gctcagcggt gacgttttga
     9601 tggcaacgca aagaaatgtg atcggcagtc agcggagtga tatccaaccc cagacggcgg
     9661 gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg
     9721 tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac
     9781 cctgttgccc tgtgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa
     9841 cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccaccc
     9901 taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt
     9961 gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc
    10021 tgcggattgc gaaccgcaga ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc
    10081 taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca
    10141 ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg
    10201 ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc
    10261 gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc
    10321 gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc
    10381 agcagtgcgt agtctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga
    10441 ctggggcact cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc
    10501 cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga
    10561 cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga
    10621 cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccaaat
    10681 cacttacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta
    10741 caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag
    10801 cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg
    10861 cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc
    10921 gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg actcccgtca
    10981 gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc
    11041 cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa
    11101 aacccgcgcg ggtggtacag tgaaactggc agacctgctg gatgaagccc tggagcgtgc
    11161 acgtcgtctg gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc
    11221 taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga
    11281 ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca
    11341 gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattaacg aagagcaact
    11401 ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct
    11461 gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg taatgtgtgc
    11521 ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc cgatcctcag
    11581 cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac
    11641 gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt aatcgatttt
    11701 tcgtgagagt gaagcctgat cagggttagc cgatcaggct tttttattgc catctaaatg
    11761 tattctgaaa acggacatgc cattgttttc tcactgttga gtaagaggcc agaagcgtaa
    11821 tatccaggcc caggaaaacg ataactgttc aatttaagga ataccgaagt gtttaaattt
    11881 cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta
    11941 aacaaccatg acacttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact
    12001 gacattaacg tgatcaaatc atgcctgcga caagaataca atctcgtcga tacgcaatta
    12061 aataaagcct atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgcaaaa
    12121 aaacttgata ctgaacaact taacttgctt aaa
//
LOCUS       DADLXX010000119        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLXX010000119 DADLXX010000000
VERSION     DADLXX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.120"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            128..997
                     /locus_tag="JNX27_003218"
     CDS             128..997
                     /locus_tag="JNX27_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884327.1"
                     /translation="MAISIKGVNTGVIRKGNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="JNX27_003219"
     CDS             complement(1200..1553)
                     /locus_tag="JNX27_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7884328.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNX27_003220"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNX27_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7884329.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="JNX27_003221"
     CDS             complement(3381..3674)
                     /locus_tag="JNX27_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7884330.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="JNX27_003222"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="JNX27_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7884331.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNX27_003223"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNX27_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7884332.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="JNX27_003224"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="JNX27_003224"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7884333.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /locus_tag="JNX27_003225"
     CDS             7589..8890
                     /locus_tag="JNX27_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7884334.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="JNX27_003226"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="JNX27_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7884335.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /locus_tag="JNX27_003227"
     CDS             9320..11017
                     /locus_tag="JNX27_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7884336.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="JNX27_003228"
     CDS             11054..11629
                     /locus_tag="JNX27_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7884337.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="JNX27_003229"
     tRNA            11736..11811
                     /locus_tag="JNX27_003229"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2924 a         3134 c         2989 g         3106 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agggtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa caccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgttagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa agaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aactatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggaccc ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aac
//
LOCUS       DADLXX010000120        11931 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLXX010000120 DADLXX010000000
VERSION     DADLXX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.121"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..2132)
                     /locus_tag="JNX27_003230"
                     /pseudo
     CDS             complement(1..2132)
                     /locus_tag="JNX27_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2276..2899)
                     /locus_tag="JNX27_003231"
     CDS             complement(2276..2899)
                     /locus_tag="JNX27_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY7884338.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="JNX27_003232"
     CDS             complement(2896..4584)
                     /locus_tag="JNX27_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY7884339.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="JNX27_003233"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="JNX27_003233"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7884340.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="JNX27_003234"
     CDS             7507..8229
                     /locus_tag="JNX27_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY7884341.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="JNX27_003235"
                     /pseudo
     CDS             complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="JNX27_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2766 a         3310 c         2942 g         2913 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg aacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagaccg tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttatcc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc taggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctgg ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaactg
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcctcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctaatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag agacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata ataattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccggcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcacccgcta aacaagccgc
     9061 cgctaagcag caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcacc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccctcacgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gcgaaacatg gcgttagtga
    11641 cacgtaaatc gacggcacca gtatcattag taccagaaga atagattgcg ccactatagc
    11701 cgcctgcgac gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag
    11761 caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca
    11821 ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc
    11881 caccgctggc attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DADLXX010000121        11785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLXX010000121 DADLXX010000000
VERSION     DADLXX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.122"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            202..537
                     /locus_tag="JNX27_003236"
     CDS             202..537
                     /locus_tag="JNX27_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7884342.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="JNX27_003237"
     CDS             630..713
                     /locus_tag="JNX27_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884343.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="JNX27_003238"
     CDS             813..1634
                     /locus_tag="JNX27_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7884344.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNX27_003239"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNX27_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7884345.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNX27_003240"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNX27_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7884346.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="JNX27_003241"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="JNX27_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7884347.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMEMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNX27_003242"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNX27_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7884348.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNX27_003243"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNX27_003243"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7884349.1"
                     /translation="MQIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="JNX27_003244"
     CDS             7279..8223
                     /locus_tag="JNX27_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7884350.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="JNX27_003245"
     CDS             8409..9791
                     /locus_tag="JNX27_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7884351.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="JNX27_003246"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="JNX27_003246"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7884352.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="JNX27_003247"
     CDS             complement(10547..10882)
                     /locus_tag="JNX27_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7884353.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKKKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="JNX27_003248"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="JNX27_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7884354.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2995 a         3010 c         2834 g         2946 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggaga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt tgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttctttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaa
//
LOCUS       DADLXX010000122        11595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLXX010000122 DADLXX010000000
VERSION     DADLXX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.123"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            139..1824
                     /gene="btsS"
                     /locus_tag="JNX27_003249"
     CDS             139..1824
                     /gene="btsS"
                     /locus_tag="JNX27_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7884355.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1821..2540
                     /gene="yehT"
                     /locus_tag="JNX27_003250"
     CDS             1821..2540
                     /gene="yehT"
                     /locus_tag="JNX27_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7884356.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2587..3057
                     /locus_tag="JNX27_003251"
     CDS             2587..3057
                     /locus_tag="JNX27_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7884357.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3098..3559)
                     /locus_tag="JNX27_003252"
     CDS             complement(3098..3559)
                     /locus_tag="JNX27_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY7884358.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3684..5687)
                     /locus_tag="JNX27_003253"
     CDS             complement(3684..5687)
                     /locus_tag="JNX27_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY7884359.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(5684..6820)
                     /locus_tag="JNX27_003254"
     CDS             complement(5684..6820)
                     /locus_tag="JNX27_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7884360.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(6813..9093)
                     /locus_tag="JNX27_003255"
                     /pseudo
     CDS             complement(6813..9093)
                     /locus_tag="JNX27_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9104..10192)
                     /locus_tag="JNX27_003256"
                     /pseudo
     CDS             complement(9104..10192)
                     /locus_tag="JNX27_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(10734..11141)
                     /locus_tag="JNX27_003257"
     CDS             complement(10734..11141)
                     /locus_tag="JNX27_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884361.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(11202..>11595)
                     /locus_tag="JNX27_003258"
     CDS             complement(11202..>11595)
                     /locus_tag="JNX27_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY7884362.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         2801 a         2943 c         3017 g         2834 t
ORIGIN      
        1 ctatagggtg attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat
       61 ttttctgcaa gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg
      121 gaagtgtggt ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg
      181 tgcgtttttt tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg
      241 caggtcacgg ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc
      301 atcatgggca cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata
      361 ggcgcggtaa tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc
      421 ggcggcttac atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc
      481 atcgttgaag gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact
      541 gataaagtct ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa
      601 atgctgatca tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat
      661 attgctgcgc caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg
      721 ctcgataaac gcgcgatgtt tgaaaaatac acttcggctt tttctgccac tgcgctgaaa
      781 gtggcagcct cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa
      841 gtggctcagg tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag
      901 aaattgctgg cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct
      961 tcgacttaca ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa
     1021 gtaccttatc gttgctcttt gcatccgcaa tgcaaactgg ggtcgactct ggtaattccg
     1081 ttgcgtggcg aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt
     1141 ttattcagtt caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag
     1201 attcttgccg ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg
     1261 cttcacgccc aagtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg
     1321 atccgccgcg acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc
     1381 aaaaacttaa agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac
     1441 gcttatctgc aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt
     1501 ccgcaagaat tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac
     1561 gccattaaac atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga
     1621 cgtgaggggc aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta
     1681 accaatgcca gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc
     1741 gatgactatg gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga
     1801 ctaccatgga gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac
     1861 gggagaacct gcgtatattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt
     1921 caaacgccgt ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg
     1981 atatccagat gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc
     2041 gcccgtatat tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac
     2101 atgcctttga ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc
     2161 gattgcgtca ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc
     2221 tgaaatttat cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg
     2281 catttgtcag cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct
     2341 ttaccgaatt gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc
     2401 agtatctggt taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg
     2461 agttgatttt gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa
     2521 aagaggcgat tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa
     2581 ggcctcatga taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat
     2641 aatgacctgg tgcgtattct ggcgctggat aatgtcgaag ccaccgcgga acagatcgcc
     2701 gtctggctac gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg
     2761 tcattcctca atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg
     2821 gagccggaac gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg
     2881 ctgaaaaccg atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg
     2941 gaaattacag cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa
     3001 tttttacgtt attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac
     3061 gggtatggcg gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag
     3121 cggctttcat caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag
     3181 aaacgttgat tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag
     3241 tcacgttttc ctgcgcgtag gtatcggtat aggttgattt ttcttcaaca cccgcgatgt
     3301 ttttgtattt ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac
     3361 caatggaggc aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt
     3421 agacataggt aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt
     3481 ctttgtcacc gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca
     3541 gcttattgaa agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc
     3601 accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag
     3661 aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg
     3721 caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat
     3781 tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt cgccagcaac
     3841 tctgcctgac taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata
     3901 agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg cagaacgctg
     3961 gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca ggcacgagtc
     4021 tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
     4081 gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaatggca
     4141 taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc accttcgccg
     4201 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
     4261 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
     4321 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgttcaac
     4381 atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg ggtagctaac
     4441 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     4501 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc ctgttcactt
     4561 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     4621 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     4681 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     4741 ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa tcgactgttt
     4801 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg ggcgtggaag
     4861 gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca gctgcagcgt
     4921 tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     4981 ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt tttgccttct
     5041 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     5101 tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc cagtacgaca
     5161 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     5221 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg cgcacagaac
     5281 aactcaatgg tgatgctttt agcgaccagc tcctgtgcgc gtttgcgggt ggcatcggga
     5341 agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt tcccgtaggc
     5401 tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
     5461 ccgctggccc cacaggtgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     5521 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     5581 ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct taacgccgtc
     5641 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     5701 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattaa
     5761 ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     5821 gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc agcaatgatg
     5881 atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca ggttgttcaa
     5941 taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct aattgcactt
     6001 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     6061 acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc atcaccgcag
     6121 agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     6181 gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac aacttgccgt
     6241 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     6301 ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc tgacgaactt
     6361 cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg cgggcggcag
     6421 ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg
     6481 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     6541 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tgaatactgt
     6601 tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt tcaccacgct
     6661 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     6721 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     6781 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     6841 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     6901 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     6961 tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc gcgtaaatcg
     7021 ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
     7081 ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     7141 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactagcg
     7201 ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag tgccaggaca
     7261 tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga aacgccgggg
     7321 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcaag
     7381 tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca cagcgtgcga
     7441 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     7501 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     7561 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     7621 gctgacgccg cctggctaag acatgtggta atgctgccga ttccacccgc gtccagcata
     7681 tcggcttcta atttatggcg tgcggcttcc tggagtgtgg caccaaagca ggcggcctca
     7741 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
     7801 gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag tattgccagc
     7861 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
     7921 gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg cagaggcggt
     7981 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     8041 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggcgca
     8101 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
     8161 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
     8221 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     8281 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
     8341 cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggg tgagataaca
     8401 acctgtaacg gcatctgcca gcgacggcaa ttctggcgtg ttaattacct gcggacattc
     8461 gcgccacatt tttgccagtg ccggagcgtg ccagccaccg cagacaacta acacgtcgcc
     8521 attattttgc tgcatcgccc atccaatcca gcggaccata aaggcttcgc gctgacgatt
     8581 gagagcatcg ccgggggagt cgccccgcag ttgggcaaaa tagtgcgcca gtgcagaggg
     8641 taatgcagtt tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc
     8701 catgcgggtg gcacgcagca gcaaggtctg gctttcctct tgcgtatcag gcgaatcgtc
     8761 ctcttcttca ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg
     8821 acgatcggct tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc
     8881 acgcccgggg gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg
     8941 gtgggctaaa aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaacac
     9001 gtatcgtggc cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg
     9061 atgccggatg ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc
     9121 gggcttgata ataagcctgc cagtgagctt ctttatgtgt ggcgacacgc tgttcgaagt
     9181 aacggcgcag acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt
     9241 ccaccaggtc ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg
     9301 cgtgggccac gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg
     9361 ttttcttttc gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg
     9421 cgtcaggcac tttatgcgga ataccgctat gggccagcaa acgcgccgaa gcactggcga
     9481 caagctccag ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac
     9541 gctttagcgc agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa
     9601 tattaaagcc ttctcgcgca tagagctgac tggcttcacc agtgagttcc ggcaccgtca
     9661 tcaccctgtc agaaagcata cctaacagac agtcttgcac ttccagcggt gtacgcgtta
     9721 tctcttcaaa acggacgatt ttgccgtcgc gcattccctg gtaaagcggg gcagggacca
     9781 gcgcttccgt tgatggacca tgattgataa gcagggcgta gttccagccg tacttgattt
     9841 gatcttcggt agtagatgcg ccgccctgga tggttaaccc ggcatcgccg ctaatagagg
     9901 ttgccagtaa ttcagacagg agagattttg cggtgctagg ttcccccacc aacatcaaac
     9961 cgcgtccggt ggcaagagtg actaacatac gttcgacagc ggagggacta atcaccactt
    10021 tacggctaat attttgcgcg ctatcgccaa ggataaaagc acgggccgca ggcaaactta
    10081 actaccaacc gggcgggcaa ggtgcgttat cattttgttt taattttgcc agttcatcgg
    10141 cgtataacac agcagcaggc ggacgctgca gatgattatt ctgcggtgac atatattcat
    10201 ccctgaaatg tattaatgaa ataaacgata ttttacgcgc aataaatgtt tggcgcatta
    10261 gttgtttgag cgaaaaatat ctggaaagca gatcacagta aataaaaaaa cctgctggtg
    10321 aggcaggttc ttttatttac tattttcagg cctaacacca ctgcctgtgc aacatcgtgt
    10381 tccaacgaac gaattataac cacctctgga cagtgttgca attaagataa tgagatttat
    10441 aatcctgact gttgggaagt tgcgccggat cgaacccatt tcaagtcctg gctaccggac
    10501 gggtggtgcc gcaggtagtg ctctgatccg gagtgcaaca tggcgactca acaaacgtaa
    10561 accagtatcg acaggcagtg taagggtaag gtgctggtgt tgtgctcctt aataaggaga
    10621 gcggatggac acgctgacac aaaagttaac cgtgctcatt gctgtactgg agctattagt
    10681 ggctctgtta cggttgattg atttgttgaa gtaacgggca aattgaaaca catctaaggg
    10741 ggaataccgt tccccccttt aataaagcta tactcgtcat acttcaagtt gcatgtgctg
    10801 cgacttgaat tatctagagt atataattct atttgttggt tgaatatatt ctgtagttta
    10861 caattaaaaa ttactattac aggttcaggt gattcgcctc gtaatttttg ctcatcaata
    10921 aactcatctc ttatataaat caacgcatca tttattagcg ggtcaacatc ctctcgatca
    10981 cgttgatgaa atgttggacg tagccggaac cgttccagtc ggtagacgga acctgtaaat
    11041 aattcagagc atttatggat ctcaagtctg aaggaaaacg gatcttcagg tacagggacc
    11101 tcataatcgt aaatagcaac cagctctttt tgcacgatca tcgctttccc cgctggtaag
    11161 tcgtgtagat aacgggcagt cgcatcactg cccgctttgt tttaatggaa aatgacgtca
    11221 ggtgcgctta acgcctcact caaatcaaac acatcaagtt cggcaaaagt gacggattta
    11281 ttgtcacggt acactcgaag agaacggaag ctcattagcg ctttgctatc aacattgtag
    11341 tggttaaaac cgggtgaaaa cttaaggacc agccggtact cacccaggtc atagacaaat
    11401 gcgtcctcat aacctgactc ccagcctttg tatcgcattc ccataagttg cccaacggta
    11461 gcggatttac cttcccagcg agtcaggctg ttactggttg actcgtcagg tgtgagcagc
    11521 accgtgcggc gcgacaactg gcgaaaaggc ggcataattt cgtaatcggc aaaaagctgt
    11581 gcaaattcac tgcgc
//
LOCUS       DADLXX010000123        11555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLXX010000123 DADLXX010000000
VERSION     DADLXX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.124"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JNX27_003259"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JNX27_003259"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNX27_003260"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNX27_003260"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7884363.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNX27_003261"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNX27_003261"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7884364.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNX27_003262"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNX27_003262"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY7884365.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /locus_tag="JNX27_003263"
     CDS             complement(3367..4701)
                     /locus_tag="JNX27_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7884366.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDSAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="JNX27_003264"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="JNX27_003264"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY7884367.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNX27_003265"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNX27_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY7884368.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="JNX27_003266"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="JNX27_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="with KbaY catalyzes the formation of
                     dihydroxyacetone phosphate and
                     D-glyceraldehyde-3-phosphate from
                     tagatose-1,6-bisphosphate; subunit Z may act as a
                     chaperone for the Y subunit; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="JNX27_003267"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="JNX27_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY7884369.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="JNX27_003268"
     CDS             9047..9826
                     /locus_tag="JNX27_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY7884370.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="JNX27_003269"
     CDS             9816..10625
                     /locus_tag="JNX27_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY7884371.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="JNX27_003270"
     CDS             10712..11146
                     /locus_tag="JNX27_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7884372.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11555
                     /locus_tag="JNX27_003271"
     CDS             11143..>11555
                     /locus_tag="JNX27_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7884373.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2755 a         3101 c         2949 g         2750 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggcgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ccaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg ctgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaagtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccga cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactacataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactcgaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcaggtcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg catcc
//
LOCUS       DADLXX010000124        11441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLXX010000124 DADLXX010000000
VERSION     DADLXX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.125"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            13..465
                     /locus_tag="JNX27_003272"
     CDS             13..465
                     /locus_tag="JNX27_003272"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7884374.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            711..1755
                     /locus_tag="JNX27_003273"
                     /pseudo
     CDS             711..1755
                     /locus_tag="JNX27_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1871..2044
                     /locus_tag="JNX27_003274"
     CDS             1871..2044
                     /locus_tag="JNX27_003274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884375.1"
                     /translation="MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDN
                     AWALSESWCGTIQ"
     gene            2093..2431
                     /locus_tag="JNX27_003275"
                     /pseudo
     CDS             2093..2431
                     /locus_tag="JNX27_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2488..3945)
                     /gene="pepD"
                     /locus_tag="JNX27_003276"
     CDS             complement(2488..3945)
                     /gene="pepD"
                     /locus_tag="JNX27_003276"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7884376.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4207..4665
                     /gene="gpt"
                     /locus_tag="JNX27_003277"
     CDS             4207..4665
                     /gene="gpt"
                     /locus_tag="JNX27_003277"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7884377.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4757..6001
                     /gene="frsA"
                     /locus_tag="JNX27_003278"
     CDS             4757..6001
                     /gene="frsA"
                     /locus_tag="JNX27_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7884378.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6059..6460
                     /locus_tag="JNX27_003279"
     CDS             6059..6460
                     /locus_tag="JNX27_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7884379.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6499..7554)
                     /gene="phoE"
                     /locus_tag="JNX27_003280"
     CDS             complement(6499..7554)
                     /gene="phoE"
                     /locus_tag="JNX27_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7884380.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7842..8945
                     /gene="proB"
                     /locus_tag="JNX27_003281"
     CDS             7842..8945
                     /gene="proB"
                     /locus_tag="JNX27_003281"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7884381.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8957..10210
                     /gene="proA"
                     /locus_tag="JNX27_003282"
     CDS             8957..10210
                     /gene="proA"
                     /locus_tag="JNX27_003282"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7884382.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10325..10400
                     /locus_tag="JNX27_003283"
     tRNA            10325..10400
                     /locus_tag="JNX27_003283"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10358..10360,aa:Thr,seq:cgt)
     gene            complement(10415..>11441)
                     /locus_tag="JNX27_003284"
     CDS             complement(10415..>11441)
                     /locus_tag="JNX27_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7884383.1"
                     /translation="WRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLH
                     FREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKT
                     RHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVV
                     MFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQG
                     LHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWAS
                     WLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEEN
                     VVYLNAR"
BASE COUNT         2748 a         2837 c         2946 g         2910 t
ORIGIN      
        1 tgaatttata ttatgaataa catacaaata agagattatc agcctggcga ttttcagcaa
       61 ctatgcgcta ttttcattag agcggttacg atgaccgtca gtcagcatta ttcaccacaa
      121 caaattgccg cctgggcgca gattgacgaa tctcgctgga aggagaaact cgcgaaatca
      181 caagtgcggg ttgcagtcat taatgcaaaa ctggtcggct ttattacctg cgttgaacac
      241 tatatcgata tgttatttgt tgaccctgaa tacacccgcc gtggggttgc cagcgctttg
      301 ttaaaacctt ttattaagtc tgaatccgaa cttacggtgg acgcaagcat aaccgcaaaa
      361 cccttttttg aacgttatgg ttttcagaca gttaagcagc agcgcgttga atgccgggga
      421 gcgtggttta ttaattttta tatgcgttat aaaccgcaac attaaatcca gcttgcaatg
      481 aaaataacgc ccgcctagta tgtgcgcgtt ttcttattcc acaaactgca aggaggtaaa
      541 tcatgacaaa tcctttatca atgactcttt gcagaccttt ccaggattaa ttcttttttt
      601 cttgccctgg attcgtctgc catttcctga ttttttaatt gttactctca ataattcaag
      661 tcgcagcaca tgcaacttga agtatgacga gtctatttac ggaatggtat atgggcaatt
      721 atattcgtcc cttatccgat gcggtattta ccatcgcatc tgatgacctg tggatcgaga
      781 gtttagcgat ccaacaatta cacaccacgg caaatttacc caacatgcag cgcgtagtgg
      841 ggatgccaga tttacacccc ggacgcggct acccgattgg cgcagcgttc ttctccgcag
      901 gccgttttta cccagcactg gtcggcaatg atatcggctg tggcatggcg ctatggcaaa
      961 cagatattct cgctcgcaaa tacaacgccg ataagtttga aaagcgatta tcagctttgg
     1021 atgacgttgc tgaagaaagc tggctggagg aaaacctgcc gtcagcattt gcacagcatc
     1081 cgtggcgcag ctcgcttggc tctatcggtg gtggcaatca tttcgctgaa ctgcaacaag
     1141 ttgatcaaat catcaacgct gaactgtttg cactggcagg tctggatgcg cagcatctgc
     1201 aactgctggt ccatagcggc tcgagggagc tagggcagtc tattttacag cggcatattg
     1261 cctcgttttc gcatcatggt ttgcctgaag gcagtgacga cgcgctggcg tttgcgcgtt
     1321 ttaatcgcca cctgatcgct ttgcgcataa tgcaacaggt taaggccacc ggaagtccgg
     1381 ttctggatgt ggcgcataac tttgttagcg cgtgtcgaat cggtgatcaa caaggggtct
     1441 tacatcgtaa aggtgccacg cctgatgact gtggtctggt ggttattccc ggttcacgcg
     1501 gtgattactc caggctggtt caggcccgtc aggagtgagg aaacattgca ttcgctggcg
     1561 catggggctg ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac
     1621 acagcgacgc aactctcgcg tactgaactt ggcagtcggg taatttgtcg cgataaacaa
     1681 ctcatcttcg aagaagcgcc acaagcctat aaatcggctg aaagtgtggt gcaatgtctg
     1741 ggcaggccgg attaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca
     1801 gtggagggaa aaaaggatga tcttgctata actctcctca gcgcaggggc cggaagaatg
     1861 ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc gacaagacgt
     1921 cgcggtaacg gtgctggaaa cagaaacagg ccgctactct gacacgctgc gttcggcgct
     1981 gatttctctg gatggcgaca acgcatgggc gttaagcgaa agctggtgtg gcactattca
     2041 gtagatttgc ccgagcccgt atcgcccaca tcacgggcgt aaaaactggt ttctgggcat
     2101 tgggcgtttt accgctgatg agcaggagca atccgatgcg atccggtatg agacgctgcg
     2161 ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca
     2221 tttggcatcc ggtattagcg tgaaggttca gtcagagcgc agccagcatg ctaacaaacg
     2281 gctggcgcga ttgctgattg cctggaagct ggatcagcag caataggaaa atagcgcgta
     2341 gctgaaatca cagcggcgaa tgttccatca ccagattgaa cgtggcaacc cgcgacgaac
     2401 gtttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta
     2461 aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt
     2521 cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg
     2581 accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag
     2641 accacattcc aggcccgcgt ggataatctg gatgttcggc gtcttgttga acaggcgctg
     2701 ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata
     2761 tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc agcgaatcca gcatgctcac
     2821 cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt ctacgttatt
     2881 gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc
     2941 ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga
     3001 tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt
     3061 ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag
     3121 tgcgtcgact ttatcagctg tgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt
     3181 acgcagtgtg ccgccgttga aatcgataag gcgcaggtcc agttcttctg catgacccgc
     3241 caggaagcgc accagcagtt tgttggcatt acccaggcca acgtggattt caccgccgga
     3301 gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc
     3361 ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat
     3421 ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg
     3481 taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg
     3541 gccgtgaacc acgttttcgt cagccagaac cgccagtgca gaggccatac caatgccgtt
     3601 atccgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat
     3661 aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg
     3721 ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag
     3781 gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat
     3841 gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa
     3901 aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt
     3961 cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa
     4021 gacaggcttg cgagatatgt attgagaata ccactttatc ccgcgtcagg gagaggcagt
     4081 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tatctataat
     4141 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     4201 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     4261 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     4321 ggtctggtac cgggggcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     4381 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     4441 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgctgtt
     4501 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     4561 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat cgaacagccg
     4621 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     4681 gcattgccgg gcgttgttct ttttaacttc aggcaggtta caatagtttc cagtaagtat
     4741 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     4801 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggtgcac aaccgcctgt
     4861 gcagtcggct cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     4921 gtggatctgg cgcggtattg acccacgtga aatcctcgac gtccaggcac gtattgtgat
     4981 gagcgatgct gaacgtaccg acgatgattt atacgatacc gtgattggct accgtggtgg
     5041 caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtacgga
     5101 agaagatccg caactcagtg gtcgacactg gctgcatgcg gctacgttgt acaacattgc
     5161 cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg
     5221 cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt
     5281 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
     5341 gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
     5401 acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt
     5461 ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc
     5521 gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
     5581 cgctaacgtt gccgtgcgtt tggcatacct tgaatcgccg cgtctgaaag cggttgcctg
     5641 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga
     5701 aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt
     5761 acgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc
     5821 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
     5881 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta
     5941 tcggaatttt gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta
     6001 aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat
     6061 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc
     6121 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
     6181 cgtcaacgtg aaaccggcac cggaagtgcg tgaattctgg ggctggtgga tggagctgga
     6241 agcgcaggaa tcccgtttta cctacagcta ccagtttggt ctgttcgata aagcaggcga
     6301 ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc acaccctgcg
     6361 tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc
     6421 ggatgatttt cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat
     6481 cacatccggc aatattcatt aaaactgata cgtcatccca accgcgacag tatcatcatt
     6541 attaatattc agcttattat cgctatccag tttgttgatt ttataatcaa caaacgctga
     6601 catatttttg ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc
     6661 accgatgccc tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc
     6721 aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc
     6781 agttattggc gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc
     6841 atcgtatttc agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag
     6901 gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc
     6961 gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg
     7021 gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa
     7081 gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg
     7141 cgccgaggaa tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa
     7201 cgcgcccagg ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa
     7261 agcgagacgc gttttttgct gtgcagtatc actctccgct ttatttccgg caaactccgc
     7321 ttcccaccga ccataaccag tcagttgatc gttaatttga gtttcacctt taaaaccaaa
     7381 acggatataa ctctggtcgc catctttact ggcgttatca ctcatataat gcatggcttt
     7441 aactttgcca tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac
     7501 agatgcagat gccacaatgc ccattaccac taatgccaga gtgctctttt tcattttcat
     7561 tcctgatttt aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt
     7621 ttttaacctg agattgttaa agatatatta cagattaatg atattctgaa aatgtggtaa
     7681 tttattaaat ctgtaataaa agcgtaaaca actgccgcta cgcttgctga tcccgcgcaa
     7741 caaaacgcca tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt
     7801 cgtttgatat catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc
     7861 tggtggtaaa actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc
     7921 atatcgttga acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta
     7981 ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag
     8041 cgactatcgc ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg attcaactgt
     8101 gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg
     8161 atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgatc
     8221 acaatatcgt tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaggtcg
     8281 gcgataacga taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc
     8341 tgctgaccga tcaaaaaggt ttgtataccg ctgatccgcg cagcaatccg caggcagaac
     8401 tgattaaaga tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt
     8461 caggcctcgg aactggcggc atgagtacta aattgcaggc cgctgacgtg gcttgccgtg
     8521 cgggtatcga caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg
     8581 aaggcatttc cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac
     8641 gctggatttt cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg
     8701 ccattctgga acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt
     8761 tctcgcgtgg tgaagtcatc cgcatttgca acctcgaagg tcgcgatatc gcccacggtg
     8821 tcagtcgtta caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg
     8881 atgcaatact gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc
     8941 gttaaggagc tggccgatgc tggaacaaat gggcattgcc gcgaagcaag cctcgtataa
     9001 attagcgcaa ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact
     9061 ggaagcacaa agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc
     9121 caatggcctt agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg
     9181 cattgccgac gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga
     9241 tggcggcgta ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat
     9301 tggcgtgatt tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa
     9361 aaccggtaat gcggtgatcc tgcgtggtgg taaagaaacc tgtcgcacta acgcggcaac
     9421 ggtggcggtg attcaggacg ccctgaaatc ctgtggctta ccggcgggtg ccgtgcaggc
     9481 gattgataat cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga
     9541 catgctgatc ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat
     9601 cccggtgatc acaggtggta taggcgtatg ccatatttac gttgatgaaa gcgcagagat
     9661 cgctgaagca ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac
     9721 ggttgaaacg ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa
     9781 acaaatggcg gaaagcggcg tgacattaca cgcagatgca gctgcgctga cacagttgca
     9841 ggcaggccct gcgaaggtgg tggcggttaa agccgaagag tatgacgatg agtttctgtc
     9901 attagatttg aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga
     9961 acacggcaca caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt
    10021 tgttaacgaa gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg
    10081 cggccagttt ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg
    10141 cccaatgggg ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat
    10201 tcgtgcgtaa ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa ggccattgac
    10261 gcatcgcccg gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt
    10321 cagggccgat atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc
    10381 gactcctatt atcggcacca tttaaatcaa taagttacct cgcatttaag taaaccacgt
    10441 tctcctcttg tgccgtattt gtgccattgc gacttataat cgcatcgatt ttgcttgcgt
    10501 gctcggtgag atgcccggct gaaaggtggg cgtatctttg aaccatttcg agagtttccc
    10561 atcctcccat ctctttaagt gcaagaagag agacaccgga ctgaaccagc cagcttgccc
    10621 aggtatgcct cagatcatgg aagcggaagt tgctaatgcc tgcccgcttt aacgctccct
    10681 tccatgcctt gttgctgtcg gttctcatct tccttaccgc tgctgttttt gttccgtcgc
    10741 ttcggtaggc aggtttggtg tggacaaata cccatctctt atggagcccc tgctgttttc
    10801 ttaatatctg gcatgcggtt tcgttaagag gaactccgat cgcattgcca gcttttgttt
    10861 catcagggtg catccatgcc attttcttat ccagatcgac ctgtgaccac tcaaggtctg
    10921 taacgttgga acggcgaagg cctgtcgtga ttgcaaacat gaccacaggg aagaagtgag
    10981 gagcaatttc tgcaaacagg cgcttcgatt cctcctctgt aagccatctg atgcgtccat
    11041 tcttaacgcg tggtgttgat attttgggtg ccctgtcaag ccatccccat tcaacagcca
    11101 tattgagaat ggcgcgaagt attgccagat gcctcgtctt cgttcctttg cttgcaagct
    11161 ttggtttata ctccggcacc ggattgccaa gtcgcaaaca cctgtcccgg ctcatctccc
    11221 agttcaggcg atggcggcgg ttttccatcc cgtctaccgc ctccattatt ttttctgttg
    11281 ttatgtcaga gagaatggct tctctgaagt gcaacatcca gaacgatata atgctcttgt
    11341 catcatcaat ggacttctta tccgatttct cacgcagcca ccgtatgcag gcttccttga
    11401 atagcttttt cggtgattcc ccgagatttt ttactctcca c
//
LOCUS       DADLXX010000125        11435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLXX010000125 DADLXX010000000
VERSION     DADLXX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.126"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JNX27_003285"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JNX27_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7884384.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="JNX27_003286"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="JNX27_003286"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7884385.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="JNX27_003287"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="JNX27_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7884386.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /locus_tag="JNX27_003288"
     CDS             3239..4579
                     /locus_tag="JNX27_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7884387.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /locus_tag="JNX27_003289"
     CDS             4657..6303
                     /locus_tag="JNX27_003289"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7884388.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="JNX27_003290"
     CDS             6426..7055
                     /locus_tag="JNX27_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7884389.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8603)
                     /gene="melB"
                     /locus_tag="JNX27_003291"
     CDS             complement(7194..8603)
                     /gene="melB"
                     /locus_tag="JNX27_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7884390.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="JNX27_003292"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="JNX27_003292"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY7884391.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="JNX27_003293"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="JNX27_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY7884392.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         3003 a         2936 c         2763 g         2733 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg acttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcta ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tccgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aattgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcac ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataaccg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcctggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctggct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggttat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaa
//
LOCUS       DADLXX010000126        11355 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLXX010000126 DADLXX010000000
VERSION     DADLXX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.127"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(353..916)
                     /locus_tag="JNX27_003294"
     CDS             complement(353..916)
                     /locus_tag="JNX27_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7884393.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(963..2324)
                     /locus_tag="JNX27_003295"
     CDS             complement(963..2324)
                     /locus_tag="JNX27_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HAY7884394.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2376..2606)
                     /locus_tag="JNX27_003296"
     CDS             complement(2376..2606)
                     /locus_tag="JNX27_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884395.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(3644..3835)
                     /locus_tag="JNX27_003297"
     CDS             complement(3644..3835)
                     /locus_tag="JNX27_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884396.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3832..4254)
                     /locus_tag="JNX27_003298"
     CDS             complement(3832..4254)
                     /locus_tag="JNX27_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY7884397.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4301..4726)
                     /locus_tag="JNX27_003299"
     CDS             complement(4301..4726)
                     /locus_tag="JNX27_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7884398.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(4699..5271)
                     /locus_tag="JNX27_003300"
     CDS             complement(4699..5271)
                     /locus_tag="JNX27_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YubH family protein"
                     /protein_id="HAY7884399.1"
                     /translation="MCCVYRMNRPAAGLTVVFCGRWSGKPGPKSAAWRMPWQKSGNDD
                     VGGVNPRLFPAGPRTEHKGSRRRLRFTRPCRAWPCGFSVMWPQRRVRAVPCLHLSRAG
                     RDARVRFAAAVTRSLLPVCRDFPVVHPLRFRGLTLQLPPAVCVRLRLPLRPVHPRLIA
                     RLLWRHGTARCRGICEQRRKTSCNMRNLSL"
     gene            complement(5695..5920)
                     /locus_tag="JNX27_003301"
                     /pseudo
     CDS             complement(5695..5920)
                     /locus_tag="JNX27_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5971..6405)
                     /locus_tag="JNX27_003302"
     CDS             complement(5971..6405)
                     /locus_tag="JNX27_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY7884400.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6419..6640)
                     /locus_tag="JNX27_003303"
     CDS             complement(6419..6640)
                     /locus_tag="JNX27_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884401.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6641..7324)
                     /locus_tag="JNX27_003304"
     CDS             complement(6641..7324)
                     /locus_tag="JNX27_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7884402.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7401..7694)
                     /locus_tag="JNX27_003305"
     CDS             complement(7401..7694)
                     /locus_tag="JNX27_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884403.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7842..8804
                     /gene="parM/stbA"
                     /locus_tag="JNX27_003306"
     CDS             7842..8804
                     /gene="parM/stbA"
                     /locus_tag="JNX27_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HAY7884404.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8807..9157
                     /locus_tag="JNX27_003307"
     CDS             8807..9157
                     /locus_tag="JNX27_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein stbB"
                     /protein_id="HAY7884405.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9280..9561)
                     /locus_tag="JNX27_003308"
     CDS             complement(9280..9561)
                     /locus_tag="JNX27_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884406.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9689..9923)
                     /locus_tag="JNX27_003309"
                     /pseudo
     CDS             complement(9689..9923)
                     /locus_tag="JNX27_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752033.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10859..>11355)
                     /locus_tag="JNX27_003310"
     CDS             complement(10859..>11355)
                     /locus_tag="JNX27_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884407.1"
                     /translation="QGLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSD
                     TKATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEAL
                     KQQILKDISLCSYEGLHVLKIEIKSVGEVCHFENRYYIRQGTATHLITDGEAISSLHR
                     LYYS"
BASE COUNT         2771 a         3073 c         2828 g         2683 t
ORIGIN      
        1 gcgtcaggga tggaagcccg ccagggcgga gacagctccg ctggctccgt tcacgacagc
       61 ccgccccgcc agggggacgc ccggaaaagg cataagcagg agaacaacaa aacagacagt
      121 aatcagagca gcagaaaaaa gaagaacagt agcagtagca gtagcagtag caggaacaac
      181 aacatcatca gtaacaggca aatgacaggt ctgaaaagac agaagcccac agcagaacgt
      241 ccgcgtaatg aaaggggcgg cgcgtaagcg cggccccttt cagcgccacc ggcgcgcggt
      301 cttttcccgg cgcgggcaga cgggcattta tcctcccgcc tgcccggatt catcaggcac
      361 gcagacgaat aatcatcgtc ggaacacggg tataagcaaa tgtgccgcac ggcagcacct
      421 cgcggacatc agaaaacggt aacagcttct cctgctggcg cggcccgttc agacagacgg
      481 caaccagaac gccccccgga cgcaacaggg aaaaagcccg caggatatgc cggatatcct
      541 gcccgtgact gaacggcgga ttcataatga tccggctgta atactgcacc ggctgccatt
      601 ccatgaagtc gccacactgt accctgacac cgttaaagtt ttcccgcaga taccggacca
      661 gcccgctgtt aatctccacc gcatcacaca tggcctccgg cgcggtatcc cgaatcgccc
      721 gcagtatggc cccggtgccg gcagagggtt ccagaatgtg atcacggttg ctgatttccg
      781 ccagcgtcac caggcgatca cagaccgggg caggagtcac aaaaagctga gattcagccc
      841 ggatttcagc aaccggcgcg gcgttatcca gccttgcgcg catcagccgg atacgttctc
      901 ttaactgact gtgcatgtca ttatctccgt catcccccgg ggatttcccg gggggatata
      961 ccttatttct gcgggatttc cgtgattttc atgtcggtga tatacacatt caccagacgg
     1021 aaattattgt ccattgtgcg gcggtagcgg tatgccccgt ggtcttctgc ttcttccaca
     1081 ctgcgcacgg ccttacagtc ccggggcagg gcggcccact gcgcctttgt catttcccgg
     1141 aaaccttccc ccggatagtt gactaccggc ggacgcttcg cggcctgact ggcgacggca
     1201 gcctcttctg ccgatggtgc tttgtagtcc gtgatgcgat cgggcgtcac tttcatcgtg
     1261 ccgctgtacc cgagaaaact gtaatttggc gttgtgactg aactcaccgc cccgttgctt
     1321 ttgttcacgc ggatgatagt cagccactcg ccccggctga aaacctgtcc gcccggctca
     1381 aaatcctgtg tccgggtaac cacgccgccg ctttcgtcca gcatggcacg ctcatagatc
     1441 aggcggttct gatagtggtt aacccagcgt tgctgatgct gaatctgccg ttcatgacag
     1501 cggatcgcaa tttcgcgggc ctgctccgtg gtgatgatgt catcatccag ggccgaccat
     1561 aacgaccggc tcccttcata ctggcttttt tctgccgggc gcggatacgt atccagcggg
     1621 aaacaggcgc tgatatggtc atgactgctg atgagttttg ccatattcag atccagcgat
     1681 tcagcccgcc acatcgtcag atatttctgc gactgtgcga tggtcttttc agccttgcgc
     1741 aaatccgctt cgattttttt gatacgacgc cagcgaacgt ccgggcgttc tttatacttc
     1801 gcgtgaagca gtgccgaccg cgcccgctcc tcccagtatt ccgcacgttc aaagagcatc
     1861 acggcacgtt tcatgccgtt ttcaatacgc tgcgcatcac gacgggcgcg gcgttcgctg
     1921 tgatgcccca caagaatggg ctgaccgggc gggatcatcg cggccagtct ttccacttca
     1981 tcaagtgcct gtgcggattc actggcccgc tttccgctgt atccggtaaa ccgctccgcc
     2041 cgtgcttcct gacgttcagc gagcgtgctg tcttcatcct caatctctcc ggcaagtgag
     2101 aggagcacgt cttcccggcc cggtgtccag gctggcgcga caaacagcgc ctgttttggt
     2161 gcccagcgaa aaccagcatc atgcactttt ttatacgtct ccgggtcaag acgtgacacg
     2221 gcatacaggc gcagtttgtt gtcatccggt gagtaggtcg cgcggtacat ctgcacaggg
     2281 ctggcggctt ccggggcggt catggtgatt gctgctaatg tcataaaatg ttctccttcc
     2341 gtctgttgcc gcccctcgcg gggcggctca cctcatcagt taaaagcgcg tttcagttcg
     2401 agtgtgcgga gcacctgccc gaaagccgtg tttttcatgg cggggatgtg tacaccacgc
     2461 ccccggtgaa aactccgggc tatgtgtcgc ccgaaggctt tcaacacggg gtcaatttcc
     2521 ggcgtgggga tgtcttcgcg tctgaaacgg ggatgacggc ggggctttac agccgctgcg
     2581 gaaggtgaaa caatgcttac agtcataaat ggattcctta tgacaaattg gttaacaatg
     2641 ccgtcaaaat tggtcgcaca attcttacgg cgtggattaa gcaggtgcct gacctgcttt
     2701 tttcatatct gcatctgacg ctttccggtg ttttcctcct tctgttcctg cgttctcatt
     2761 acctgtcagc cggatgactg acagaacaaa taaatgcgta ttgccggagc ctgaaataaa
     2821 tcctgatttt taaagaacat cacccgttga gtttcgggag ccgcggtttt gacgtttcgc
     2881 gggaacctgc tgctgtattc actgtggtat gaacttcacc cgtttgagaa ccttcgccgc
     2941 aaggggcatc actgccacgg cctgacgcac ctgtcctggg cagaacgcaa aattttttac
     3001 cgggttttgg gtaaaaaatt ttgtgggctg ttcatttacc ctggacagtc agtgcggcag
     3061 gttgtggctg tatcagcccc cggcgacagg gactcatacg ggtgtgtggc acccggcagg
     3121 caatgagcag gggcccgccg caacgacgga gagcgcgtgc gcgaaacggc gttgcccctt
     3181 acatcccggc ggccttttgc cgggatgccg ggagcgcagg ccacggcgcg acagaggcgg
     3241 cgcgaccgga acggtcgctg caagccaggg gttttcacgc atcggaccgt agcggcagcc
     3301 cgaaaccgcg ccagcgggtt cggcggagac tggcaggtgc gccagcagct gcctgtcgga
     3361 gtcgggcctg acgaccggat gccgccaggc agccgggagc cgaaggtgcg cccggcagac
     3421 agaacaacac tgcagtagtc agaatcagag cagtagataa aggaacaaca acatcaggaa
     3481 cagttccata acataagaaa gaaacactcc tgagtcagac acatcacaga cgggaagggt
     3541 aacgggcgac gcgccagcgt ggcccgcttc ccttccggca ggcgtcagcc tgccaggggt
     3601 tttcgcgcgg gctgacaggc ggcctccacc gcctgcccct gtatcacctg tttatgctgc
     3661 cctgaaggat gtagtgaccg ccgaggaata cagcctgctg attcgccaca aaatcgcaga
     3721 tttcatcaac aatctgcgca gtggtgagcg gtttctgccc catttcacga cgccgcgcat
     3781 taatcacggc tttgtaattg cgcagaattt cgcgggtttc tgtgctgata ttcatgacat
     3841 gtgctccccg tccagcgcct gtaacacaac gtccattgct tcgcgttctt ccctgacgaa
     3901 tgcgtcaccg tcgcccccgt tctcgctccc gacggcatac aggcgcttca tttcactgcc
     3961 tgccagcgcc atcacttttt ccagcatcac cgccggaact gtcacctgac ggctggcgcg
     4021 cttttcttcc atcagctcac gaacaacgtc gtgaacaatg ctgacatccg caccatgcgc
     4081 aaaggcatcg tccagtcgct gcatgacggt ttccagctca tcgtctgtca gactccagtc
     4141 ctgggctatg tgacgcacat ccccacgggt cagcaccacg gcaaaaacgc attcactgtc
     4201 cggcacgtct gtgttcagca cttcgcggat aatttcttta acagtacagt acatgttctc
     4261 gtctccttta caggggcggc attgccgccc cgtccgttaa tcagtccagt gccgcgtaaa
     4321 tcgcgttgca ttcggggtga aattcgatgt gacgccacaa ctgcgcatcg cgcatccggt
     4381 aaagctgggt cagtcctgca tccccgctgt cgtggtacag ccacaactgg cggttaatca
     4441 caagggaagt cagaatgatg cctgcggcat cagcactcac ggtacggtca aaccagtttg
     4501 caccgttcac catgtggaaa cggtcgccat ccggcatcat gtaaccgcca ccgtccggca
     4561 ggcggatgaa gtgccagaag cctccctgat attccggcat cattttttgc gccagcgcgt
     4621 acacatacgc ctcggcatac agaaaatcgt tgccaaacag atacggtaga aaggagagac
     4681 ggtcgcactc ttcaacgttc agagtgacag gtttcgcata ttgcatgatg ttttcctccg
     4741 ttgttcacat attccgcgac accgggcggt gccgtgcctc cataaaagcc gggcgataag
     4801 gcggggatgc acagggcgca agggaagccg cagcctgacg cacacggctg gcgggagttg
     4861 gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc agacgggaag
     4921 gagggaccgg gtgaccgcag cggcgaagcg tacccttgca tccctgccag cccggctaag
     4981 atggaggcac ggcacagccc gcacacgacg ctgaggccac ataactgaaa aaccacaagg
     5041 ccatgccctg catggccggg taaagcggag ccggcggcga cttcctttgt gctctgtccg
     5101 gggaccggca ggaaaaaggc ggggatttac cccgccaacg tcatcatttc cggatttttg
     5161 ccagggcatc cgccatgctg cggatttcgg cccgggcttc ccggaccacc tcccgcagaa
     5221 caccacggtc aaaccagcgg cggggcggtt catccggtat acgcagcaca tcgcaggcca
     5281 gcccactgga caggatatgc tcaatacaat cgctgaccag gtacatttcc accgacgaaa
     5341 aacgtgctcc ccggttcacg atggcatcga gcaccacggg caggttttcg tcatcttcat
     5401 cgagccagca ggggtgatcg ctttccttca caaccagacg aaagcggcgc gtctggcgag
     5461 tcagcaacgt ctgaccggtg gtggtcagcg ccgcccggat acgccgcgca tcttctgcca
     5521 cccggcaact gacactgcgt accgcatcgc gcaccgccgc aagtacagcc ctgatttcgt
     5581 cttcggtcag ccattctccg gcatacggga aagacacctg cgttccgtct gccggaagta
     5641 ccggcagcga ctgccactga ctgccggaca gtaccagttc cgccccggtt ccggtcagtt
     5701 tctgaatggc ggaggccagg agcggcggca gtacgccgcc gtgacggttg ataagtaacg
     5761 tcgccagccg gggcgcatcc agcagaacat aaatgctgat ccagcccgga tacttcgggt
     5821 tacgctggtc aacatccgtc tcttccggcc agccctgcgc cagcagcagg ttgcgggcgt
     5881 gacgacaaat cagtgcgttc agcgtgctgt tttcgttcat gattaagcgt tctccctgta
     5941 tcgccgcccg cagggcaggc ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag
     6001 cagggtgcgg gcctgattaa ccacctcctg acggcgggtg acgcattccg ggacggcgag
     6061 gccatgatcc cgggcggccc attcacgttt ccacaatgcc tgttcagcgg tctgaatcag
     6121 ggacgccagc agttcagcag ggacggtcac caggcggttt tcgctctcgt tgcggataat
     6181 ctccatcacc gcagcggaag aaataccgga ctcaatccgc tggtcttccg ggatgtcctc
     6241 caggcgtgcc aggacggtcc tgatttcatg accggtcaga gaaatttcca taccgtcagc
     6301 cagtgcctga atctcttccg gggaccagat aaccagcatc agcggcatgt ctcccggata
     6361 ctttactgcc agctcccggc atagcgtttc gcatgttccg tacattctcg tctccttctc
     6421 aatctttaaa gcacgcttcc agttccagaa tttcggtata tgcctccatc aggaggcggt
     6481 ttgcgcgaaa cagcgtctga cgcaccagac gttccggcag ggtgtcggca ttaaagctgt
     6541 accgctgctc catctcattc atggttgtac gcaggtcgca catggcattg gccacctccg
     6601 ccacttcggc gcgcagtttt tcgtgtcctg catagttcat ttacgcagcc tccggcataa
     6661 accagtcatc attcgcggcc ccctgctgca tggcccgttg cacggcggca aggcgttgct
     6721 gcccggcacg gtgatactgc tcaagcagct cgataccgat ataccggcgt ccggactgga
     6781 gggcggcgac gcaggttgag ccgctgcctg caaacgggtc cagcacaatt gcgttcgggt
     6841 gtgtgaagct ctcaatcagc ggttgcaggc tggtaacggg cttttccgtc gggtgatggc
     6901 gattgcccga atatttccag cccagcacgt ccggcagcgg gttttgtggc agacgtggac
     6961 ggccttttgc caggatgtag gcacattcgt ggcgatagcc cacatatgcg gccttcgatg
     7021 tgtagttttt ggtgaagacc aggtgaccaa caacgctgaa tcccgcattt ttccaggcgg
     7081 ccataaagcg atcgacgcgg ttccagccat agaagctcac cattaacgcg tcttttttga
     7141 gtacgcggta catttcatta caggctggtt gcagccattc atcggtttta tcgccggcga
     7201 tggtgcgccc ggaacggtca cggaaaccga cgagatacgg cgggtcggtg aggatgaaat
     7261 cgacagcatt gcccggaaac gtggccataa cacgcacgca gtcacccagg ataaaacggg
     7321 acataacagc actccttctc ttacagaggg caccgggagg gcacccctgt ggtgcccgtt
     7381 ccccgctgcc tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac
     7441 cgcagccgtc gtctttttga cggcgaggat atgccgcgaa agcggcttga agggcgttca
     7501 tcagaaaagg gcgacgggct ggcgtacact gtcaccaacg ccagccggga gcggtaaaag
     7561 cgcccgtccc gtgcccggcc ggacgcggga cactgaccac aggcagcgtt cctgccgcgc
     7621 cgcgtcagcc ggcgcagtaa aaagaggagc ccggcgacat cttttgttac ccgccaaaca
     7681 aaacccaaaa acaacccata cccaacccaa taaaacgcca aaacaagaca aataatcatt
     7741 gattgatggt tgaaatgggg taaacttgac aaacaaaccc acttaaaacc caaaacatac
     7801 ccaaacacac accaaaaaaa acaccataag gagttttata aatgttggta ttcattgatg
     7861 acggttcaac aaacatcaaa ctacagtggc aggaaagcga cggaacaatt aaacagcaca
     7921 ttagcccgaa cagcttcaaa cgcgagtggg cagtctcttt tggtgataaa aaggtcttta
     7981 actacacact gaacggcgaa cagtattcat ttgatccaat cagcccggat gctgtagtca
     8041 caaccaatat cgcatggcaa tacagcgacg ttaatgtcgt tgcagtgcat cacgccttac
     8101 tgaccagtgg tctgccggta agcgaagtgg atattgtttg cacacttcct ctgacagagt
     8161 attacgacag aaataaccaa cccaatacgg aaaatattga gcgtaagaaa gcaaacttcc
     8221 ggaaaaaaat tacattaaat ggcggggata cattcacaat aaaagatgta aaagtcatgc
     8281 ctgaatctat accggcaggt tatgaagttc tacaagaact ggatgagtta gattctttat
     8341 taattataga tctcgggggc accacattag atatttctca ggtaatgggg aaattatcgg
     8401 ggatcagtaa aatatacgga gactcatctc ttggtgtctc tctggttaca tctgcagtaa
     8461 aagatgccct ttctcttgcg agaacaaaag gaagtagcta tcttgctgac gatataatca
     8521 ttcacagaaa agataataac tatctgaagc aacgaattaa tgatgagaac aaaatatcaa
     8581 tagtcaccga agcaatgaat gaagcacttc gtaaacttga gcaacgtgta ttaaatacgc
     8641 tcaatgaatt ttctggttat actcatgtta tggttatagg cggtggcgca gaattaatat
     8701 gcgatgcagt aaaaaaacac acacagattc gtgatgaacg ttttttcaaa accaataact
     8761 ctcaatatga tttagttaac ggtatgtatc tcataggtaa ttaatgatgg acaagcgcag
     8821 aaccattgcc ttcaaactaa atccagatgt aaatcaaaca gataaaattg tttgtgatac
     8881 actggacagt atcccgcaag gggaacgaag ccgccttaac cgggccgcac tgacggcagg
     8941 tctggcctta tacagacaag atccccggac ccctttcctt ttatgtgagc tgctgacgaa
     9001 agaaaccaca ttttcagata tcgtgaatat attgagatcg ctatttccaa aagagatggc
     9061 cgattttaat tcttcaatag tcactcaatc ctcttcacaa caagagcaaa aaagtgatga
     9121 agagaccaaa aaaaatgcga tgaagctaat aaattaattc aattattatt gagttccctt
     9181 tatccactat caggctggat aaagggaact caatcaagtt attttcttac cagtcattac
     9241 ataatcgtta ttatgaaata atcgtttgca ctgtctctgt tattcaggca atttcaataa
     9301 aggcacttgc tcacgctctg tcattttctg aaactcttca tgctgcattt cgatttttct
     9361 tctaaaatca taacttgcat acctgttctt tttatgtata tccagatcta tttttgtgac
     9421 gagttcaaac cacaatgata gcactcccca tgcagcgtta tgaaacattt ccgcaacaga
     9481 tttagagtct gtattaaccg cttcagtcat gtagtcgcta atacgttctg cagtgatttt
     9541 cgttatttca tcgtaattca ttttatcccc tacaagacta tttaatggaa tgaaaaatat
     9601 ttggtctgat aacaatgctc ctgaattaac acctgcaggc tatgttaaag ttgccctcac
     9661 acacaatcta cctgttttct cgtgtgtgtc acaggcgcaa tacataaata gtctggccat
     9721 taagttcgta acggatttcg tactcgccag caaaaatccg tctgacatcc ctggtttcaa
     9781 actgaaacaa ctgttcatcc catacgtgga tgagttaaca gaatgaccgg cgcctgtgtc
     9841 agggactaca ccgttttggc tgcagctggt ttactgacca acgccagaaa atcatataac
     9901 tgcgtcaatg tcagcgtatc cagcgcgact gtattatttt tctctgcaac cagaatattg
     9961 cattaggtta ttaccaggtg ttctaatgta caggtaaaga cgttctttat aatctgaaat
    10021 ctctactttt aatccctcag actttgcctg ctgaaagccc tcgtcaaggt gcgcttttat
    10081 taggttaatg tctggatgtc ttgtatcggg ggcaattctg tattggtgta acgatggggt
    10141 ggatgttccc tgcaataaga cctccagata gagattggca caaccattcc cattatttct
    10201 aatagtttat tggtaatcac aaactgtata tttagccatt atattgaatc ctgtgaagta
    10261 aacggcgacg gctcaaaatt ttatcaaacc taagataatt ctaccatcaa agttaataat
    10321 ttgaatttat atgacggctc tatggggggg taaaaatccc cgtgcagaga ggcgattttc
    10381 agagttgaaa acgtcgtcgt taagaacacc aaagtcagta ccaccaaccc ggaaagtagc
    10441 ttcatgcacc gggacaacag cagttttggt gtccgaaatc gtacaattct gtacatctac
    10501 gcaaggaaat gtacggaagt aagccaaatg gttatcggac ggtcggaaca tttcgtacag
    10561 cctttacgtt cagtgagtta gctggcctag atgtaaccac tcgttcaact caattctctt
    10621 agcgtgggaa aatttccgtt ttccaagcgg ccccctcaag cggacccctt atagtacaat
    10681 aattttcgat atccaaactg cccccaatta catctccagt ctctggcagc agagatcata
    10741 tatttccatt gccgccgtag ccacgatatt ctggctattc acaaaaacaa tttagcccca
    10801 aagcgcagta aaaaagtaca ctatgaaggt gtatgatacc ttcatagtgc ttaagtaatt
    10861 atgagtaata taatctgtgt aaactactaa ttgcttcacc gtcagtgatt aaatgcgtag
    10921 ctgttccttg tcttatataa tacctatttt caaaatgaca gacttctccg acacttttta
    10981 tttcgatttt taaaacatgc agtccttcat atgaacataa tgaaatatct tttagtattt
    11041 gttgtttcaa agcctcatta aaattaaatg attctatttt ttgttttatg aacagaaagt
    11101 aattatctat gttttttgat tgaattacag cttcatgatc aattccattg atataatagc
    11161 cattgcactc aataggggta acaccatata aagtcttaac tctgtctgcc gtagctttgg
    11221 tatcggaaac accaactaaa atataaccat tagattctct accgatgtta tttatggcga
    11281 cagccgtttg gactatatta atgaaacatt cttcgtctat tttattctcg cctgagagaa
    11341 taaataatcc ctgtt
//
LOCUS       DADLXX010000127        11183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLXX010000127 DADLXX010000000
VERSION     DADLXX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.128"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNX27_003311"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNX27_003311"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7884408.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /locus_tag="JNX27_003312"
     CDS             complement(721..1749)
                     /locus_tag="JNX27_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7884409.1"
                     /translation="MAAAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /locus_tag="JNX27_003313"
     CDS             1924..3516
                     /locus_tag="JNX27_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7884410.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="JNX27_003314"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="JNX27_003314"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7884411.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4801..5802
                     /gene="add"
                     /locus_tag="JNX27_003315"
     CDS             4801..5802
                     /gene="add"
                     /locus_tag="JNX27_003315"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7884412.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5838..6878)
                     /locus_tag="JNX27_003316"
     CDS             complement(5838..6878)
                     /locus_tag="JNX27_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7884413.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7121..7246
                     /gene="blr"
                     /locus_tag="JNX27_003317"
     CDS             7121..7246
                     /gene="blr"
                     /locus_tag="JNX27_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7884414.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7519..7734
                     /gene="ydgT"
                     /locus_tag="JNX27_003318"
     CDS             7519..7734
                     /gene="ydgT"
                     /locus_tag="JNX27_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7884415.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7820..8260
                     /locus_tag="JNX27_003319"
     CDS             7820..8260
                     /locus_tag="JNX27_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7884416.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8337..8918
                     /gene="rsxA"
                     /locus_tag="JNX27_003320"
     CDS             8337..8918
                     /gene="rsxA"
                     /locus_tag="JNX27_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7884417.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8918..9496
                     /gene="rsxB"
                     /locus_tag="JNX27_003321"
     CDS             8918..9496
                     /gene="rsxB"
                     /locus_tag="JNX27_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7884418.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNX27_003322"
     CDS             9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNX27_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7884419.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2594 a         2885 c         2817 g         2887 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagtttact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggcagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4741 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4801 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4861 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4921 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4981 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     5041 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     5101 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5161 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5221 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5281 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5341 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5401 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5461 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5521 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5581 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5641 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5701 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5761 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5821 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5881 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5941 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     6001 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     6061 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6121 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6181 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6241 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6301 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6361 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6421 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6481 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6541 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6601 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6661 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6721 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6781 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6841 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6901 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6961 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     7021 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     7081 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7141 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7201 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7261 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7321 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7381 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7441 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7501 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7561 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7621 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7681 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7741 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7801 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7861 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7921 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7981 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     8041 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     8101 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8161 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8221 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8281 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8341 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8401 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8461 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8521 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8581 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8641 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8701 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8761 tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt gctgtggctg
     8821 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8881 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8941 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     9001 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     9061 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9121 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9181 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9241 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9301 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9361 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9421 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9481 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9541 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9601 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9661 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9721 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9781 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9841 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9901 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9961 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
    10021 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
    10081 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10141 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10201 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10261 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10321 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10381 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10441 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10501 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10561 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10621 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10681 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10741 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10801 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10861 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agctgctcgc cttgaacgac
    10921 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10981 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    11041 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    11101 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11161 ccgttgaagc agctatcgcc cgc
//
LOCUS       DADLXX010000128        11093 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLXX010000128 DADLXX010000000
VERSION     DADLXX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.129"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            215..2920
                     /gene="malT"
                     /locus_tag="JNX27_003323"
     CDS             215..2920
                     /gene="malT"
                     /locus_tag="JNX27_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY7884420.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWNLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQIARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2963..3769)
                     /gene="rtcA"
                     /locus_tag="JNX27_003324"
     CDS             complement(2963..3769)
                     /gene="rtcA"
                     /locus_tag="JNX27_003324"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY7884421.1"
                     /translation="MGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADG
                     PSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVS
                     PVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSLHEQNIHNLPRDQ
                     GPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASPAAVGEYLADQL
                     VLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTRVSIE"
     gene            complement(3844..5070)
                     /locus_tag="JNX27_003325"
     CDS             complement(3844..5070)
                     /locus_tag="JNX27_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7884422.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5259..6857
                     /locus_tag="JNX27_003326"
     CDS             5259..6857
                     /locus_tag="JNX27_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7884423.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKAAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6839..7597)
                     /locus_tag="JNX27_003327"
     CDS             complement(6839..7597)
                     /locus_tag="JNX27_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7884424.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNX27_003328"
     CDS             complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNX27_003328"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7884425.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNX27_003329"
     CDS             complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNX27_003329"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7884426.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9005..10510
                     /gene="glpD"
                     /locus_tag="JNX27_003330"
     CDS             9005..10510
                     /gene="glpD"
                     /locus_tag="JNX27_003330"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7884427.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10779..11009)
                     /locus_tag="JNX27_003331"
                     /pseudo
     CDS             complement(10779..11009)
                     /locus_tag="JNX27_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         2679 a         3037 c         2841 g         2536 t
ORIGIN      
        1 gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
       61 ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
      121 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
      181 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aactaagtcg
      241 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
      301 gaacaacttc cggctggcgc tgatcacgag tcctgcgggc tacggaaaga ccaccctcat
      361 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg aaggtgataa
      421 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg cagcaggcaa ccaacggtca
      481 ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
      541 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
      601 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
      661 accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
      721 tctgcgtgtt cgtgaccaac tgctggaaat tggtagtcag caactggcat ttacccatca
      781 ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg cagaaagcag
      841 tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg ccctctccgc
      901 ccggcagaat acccactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
      961 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
     1021 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gccctcatca cccgtgtgac
     1081 cggcgaagaa aacgggcaaa tgcgtctcga agagattgag cgtcaggggc tgtttttaca
     1141 gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggca acttcctgcg
     1201 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
     1261 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
     1321 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg aatttgttca accatagcga
     1381 actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc
     1441 gcagttagtg ttattgcagg cgtggctaat gcaaagccaa catcgctatg gcgaagttaa
     1501 caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
     1561 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
     1621 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
     1681 ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc gctcactggc
     1741 gctagtgcag caaaccgaac agatagcacg ccagcacgac gtctggcact acgccttatg
     1801 gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
     1861 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagc tgccaatgca
     1921 tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc tggatgaagc
     1981 cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac agcaacagct
     2041 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
     2101 tagccagttg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
     2161 ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata aagccgccgc
     2221 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
     2281 tcaatggcgc aacattgccc gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat
     2341 tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg
     2401 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
     2461 cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagtc attttgtcat
     2521 cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caacttaata cgctgccgga
     2581 actggaacag catcgcgcgc agcgtattct gcgagaaatc aatcaacatc atcggcataa
     2641 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccgg aagtgccaga
     2701 actaatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatctactc
     2761 cggttacagc aatgaacaaa ttgcgggcga actggaagtg gcggcaacca ccatcaagac
     2821 gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca
     2881 cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga gtttagccgg ataatgcatt
     2941 ggatccggtt tacatcttta catcattcaa tactcacccg cgttacgcca tctgtttcta
     3001 tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc agcagatggc
     3061 atgaaggatg ggcgaccgta aattctcccg cgcccgccag cgccatcggc agtaccagtt
     3121 ggtcggcgag atattccccc accgccgctg ggcttgccag gtagcgtttc acctctttca
     3181 ctaactgcgc ggctaccacc tcggcactga cgcgcttttc accgacgacg aaaaagcgtt
     3241 cagtgatatt ttcactttcg acttccagcg agacggtatt acccggcccc tggtcgcgcg
     3301 gcaggttatg aatattctgt tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac
     3361 gctcagcaac atggcgcggc acaccagcta atagaacttc tccacgcatc tgcacaatgt
     3421 tcccgcgctc gccaagttgc aaggtgctaa acgatgccac gggcgagact tccgttgcca
     3481 ccacaccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc
     3541 ctattttcgc cagcagcggc tccagcaccc ggcggataaa atccgcaggc ggcgccgacg
     3601 ggttatcggt gccgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg
     3661 gcagcaccgt ttgcagcacc agcgtacaac ttccggcgct accgatagca aagcggtaat
     3721 cgccgccgcg cacggtgcca ggccggaaga ccagacgctg cgatcccagc tcagcgccga
     3781 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
     3841 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
     3901 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
     3961 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
     4021 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
     4081 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
     4141 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
     4201 aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
     4261 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
     4321 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
     4381 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
     4441 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
     4501 tccacgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
     4561 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
     4621 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
     4681 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
     4741 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
     4801 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
     4861 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
     4921 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
     4981 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
     5041 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
     5101 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt ttaatttatt
     5161 gtttctaaaa agattatatc tttatgtccg taaacggaga tttcccgcaa agccgattta
     5221 ccgataatga aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg
     5281 gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc
     5341 gtccgtcact ctgtatatgc cagcaagaat cgttggtcat cgatcgactg gaattattgc
     5401 acgatgcccg ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc
     5461 cagaaacaga agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg
     5521 tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact
     5581 atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg
     5641 aagcgcgtta tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc
     5701 cgcgcggccc aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg
     5761 ccagccgctt tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca
     5821 cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc
     5881 gcgcgccgat tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca
     5941 tcttcgagtt aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg
     6001 ccaccctgcg cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa gccgcgttta
     6061 ccggggcgcg ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc
     6121 ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag
     6181 agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg
     6241 ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt
     6301 acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata
     6361 ttgaaccgaa cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc
     6421 gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggctacat
     6481 ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta
     6541 gcggacgcat cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc
     6601 aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc
     6661 tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt
     6721 ctgctgccgg acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg
     6781 cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaggcc gtgcaggatc
     6841 agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg
     6901 gggcgtcggt gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac
     6961 ggccaaattt tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc
     7021 gcttggtacg aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc
     7081 tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc
     7141 caatgatccc gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt
     7201 cttttaccat caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt
     7261 gattgagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg
     7321 agccattggg gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg
     7381 tggctttacg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat
     7441 gatggcgcag gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg
     7501 ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca
     7561 gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt
     7621 cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg
     7681 atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag
     7741 atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg
     7801 ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac
     7861 ggcccgctga atttttgctg cacatagccg cttaacaggg cgctgataag cgtaatgaca
     7921 attagcttac cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc
     7981 aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag
     8041 aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg
     8101 ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc
     8161 ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg
     8221 ccggtatggc ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga
     8281 aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg
     8341 ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac
     8401 gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat
     8461 tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact
     8521 gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca
     8581 gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag
     8641 tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct
     8701 gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt
     8761 tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc
     8821 tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat
     8881 aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg
     8941 attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg
     9001 cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc
     9061 ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg
     9121 cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta
     9181 tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc
     9241 gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg
     9301 gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg
     9361 atcaattggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt
     9421 cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt
     9481 ggtgcgtaaa ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa
     9541 cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc
     9601 gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca
     9661 tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt
     9721 gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat
     9781 cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc
     9841 gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca
     9901 ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct
     9961 gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca
    10021 tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg
    10081 aaaactggca gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc
    10141 gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc
    10201 cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacgcgcg
    10261 cacttacggc agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg
    10321 ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg
    10381 ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc
    10441 ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct
    10501 ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga
    10561 caaacacaaa actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat
    10621 tgaatttgca tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa
    10681 gcgcactttg tcaaaaatct aacctacttt ttaattcagg gaattaccgc aaagcccacg
    10741 ccatcatacc caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc
    10801 attaatgcgc ccagcgtcag cataaaaaca ttgagcagaa ggtaaattgt tggcgtaaag
    10861 agtaaattgc aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct
    10921 gcaaaaaatt gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc
    10981 cattttctat tttgcattat tctttccatt ctttctgaat ggcgaaatta tactcccctt
    11041 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tgg
//
LOCUS       DADLXX010000129        10815 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLXX010000129 DADLXX010000000
VERSION     DADLXX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.131"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            93..1178
                     /locus_tag="JNX27_003332"
     CDS             93..1178
                     /locus_tag="JNX27_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7884428.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1249..1857
                     /locus_tag="JNX27_003333"
     CDS             1249..1857
                     /locus_tag="JNX27_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7884429.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1955..3346
                     /gene="selA"
                     /locus_tag="JNX27_003334"
     CDS             1955..3346
                     /gene="selA"
                     /locus_tag="JNX27_003334"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7884430.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3343..5187
                     /gene="selB"
                     /locus_tag="JNX27_003335"
     CDS             3343..5187
                     /gene="selB"
                     /locus_tag="JNX27_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7884431.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5377..6528
                     /gene="yiaY"
                     /locus_tag="JNX27_003336"
     CDS             5377..6528
                     /gene="yiaY"
                     /locus_tag="JNX27_003336"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7884432.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6693..8231
                     /locus_tag="JNX27_003337"
     CDS             6693..8231
                     /locus_tag="JNX27_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7884433.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8450..8593)
                     /locus_tag="JNX27_003338"
     CDS             complement(8450..8593)
                     /locus_tag="JNX27_003338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884434.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            8776..9099
                     /locus_tag="JNX27_003339"
     CDS             8776..9099
                     /locus_tag="JNX27_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7884435.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9105..10241
                     /locus_tag="JNX27_003340"
     CDS             9105..10241
                     /locus_tag="JNX27_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7884436.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10238..>10815)
                     /locus_tag="JNX27_003341"
     CDS             complement(10238..>10815)
                     /locus_tag="JNX27_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7884437.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2659 c         2977 g         2602 t
ORIGIN      
        1 atcgccgcac tttctgaccg aatttcccgg ctggagcatc aactcgccgc cgagaaaaag
       61 actgactatt ccacgttccc ggagatctaa ttatggatct attgattgtt ttaacttacg
      121 tggcgctggc gacggcggcg ctgggaggcg tgtttctggt gagtggtttg attttgttga
      181 tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg atccctatca
      241 cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag cttattcaaa
      301 agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt gacagacttc
      361 aggctgacct gatgacggcg acgcataata taaagacgct gcgtgcgcag ctcactgaag
      421 cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa aattatcaac
      481 gttacctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac atcgacgatg
      541 cgcggcaaaa tttcctcgcg caggatgcgt tagtgaaagg ctcggtggcg gagcaggcgc
      601 agatccagag tcagctcgac agtatggtca acggcgagca atcgcagatt gtgagcttaa
      661 gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc gcaccaagca
      721 atggctacgt cactcaggta ctgatccgcc caggtacata cgcagctgcc ttgccgctgc
      781 gtccggtgat ggtcttcatc cccgagcaaa aacggcaaat tgtcgcccaa tttcggcaaa
      841 actcgctgtt acgtctgaaa cctggcgatg atgcggaagt ggtgtttaac gcgctacctg
      901 ggcaggtgtt ccacggcaaa ttgactagta ttttacctgt cgtgccaggc ggttcttatc
      961 aggcgcaggg ggtattgcaa tcattaacgg tcgttcccgg cacggacggt gtgctgggaa
     1021 ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc tacgcccagg
     1081 tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg ctgctaagaa
     1141 tgaccagttg gatgcattat ctttatttgg atcattgaga cttatatcac ctgtgttttg
     1201 cgtgagcctg agccatactg atatttttcc tcgcacaaag gactcggcat gaaactcgtc
     1261 ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc tgttgttaga aaagggcata
     1321 actttcgaat ttattaatga actgccctat aacgcggata acggcgtggc gcaatttaac
     1381 ccgttaggga aagtgccggt gctggtgacc gaagagggcg aatgctggtt tgattcgccg
     1441 atcatcgctg aatatattga gttaatgaat gtcgctccgg cgatgttgcc gcgcgatccg
     1501 ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg atggcattat ggatgccggg
     1561 ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc agtctgaaga tgaattgtta
     1621 cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc
     1681 acactcaaaa ccgatacggt caatctggcg actatcgcca ttgcctgtgc tgtcggatat
     1741 ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc gcccacactt agtcaaactg
     1801 gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc
     1861 gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg gttatgttaa acgcccttct
     1921 ccgtgtgaga gggccttgat cagccaggtt tcctatgaca accgaaacgc gttccctcta
     1981 tagtcaactt ccggctattg atcgcttatt acgcgatagc tccttccttt ctttgcgtga
     2041 tacttatggt cacacccgcg tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga
     2101 agtgattcgt ggcagccaga cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga
     2161 tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac
     2221 cgtgctgcat accaaccttg ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc
     2281 gcaggctatg cgttcgccag tgaccctcga gtacgatctg gacgacgccg gacgcggaca
     2341 tcgcgatcgg gcgctggcgc agctgctgtg ccgtattacg ggggcggaag atgcctgtat
     2401 cgtcaataac aatgcggcgg cggtgttatt gatgttggcg gccactgcca gcgggaaaga
     2461 ggtggtggta tctcgcggcg aactggtgga gattggcggc gcgtttcgta ttcccgatgt
     2521 tatgcgtcag gcaggctgca ccctacacga agtagggacc accaaccgca cgcacgcgaa
     2581 tgattatcgt caggcggtga atgaaaatac cgcactgttg atgaaagtac ataccagtaa
     2641 ctacagcatt caggggttca ccaaagcgat agacgaagct gaactggtgg cgctcggcaa
     2701 agagctggat gttccagtgg tgactgattt aggcagtggc tcgctggtcg atcttagcca
     2761 gtacggtttg ccgaaagagc caatgccgca ggagttgatt gcggcgggcg tcagtctggt
     2821 gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca gggattatcg ttggtaaaaa
     2881 agagatgatc gcccgcctgc aaagccaccc gctgaagcgt gcattacgcg cggataaaat
     2941 gaccctcgcg gcgctggaag ccacgttgcg tctttattta caccctgaag ctctgagtga
     3001 aaaattaccg accctgcgcc tgcttacccg cagcgcagag gtcattcaaa tccaggcaca
     3061 acgtttacag gctccccttg tcgcacatta cggcgcggag tttgcggtac aggttatgcc
     3121 atgtctttcg cagattggca gtggttcgct gccggttgat cgcctgccga gcgcggcatt
     3181 aacgtttaca ccccatgatg gacgcggtag ccaccttgag tcattagccg cccgctggcg
     3241 tgaattgcca gtgccggtga ttggtcgtat ttatgacgga cgattgtggc tggatttacg
     3301 ctgccttgaa gatgagcaac ggtttttgga gatgttgttg aaatgattat tgcgactgcc
     3361 ggacacgttg accacggcaa aacaacctta ttgcaggcga ttactggcgt aaatgctgac
     3421 cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc tcggctatgc ctactggccg
     3481 cagccggatg gtcgcgtgcc tggttttatc gacgttcccg gtcatgaaaa gtttctttcc
     3541 aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac
     3601 ggcgtgatgg cacagacccg tgagcatctg gcgattttgc agctgaccgg taacccgatg
     3661 ctgacagtgg cgctgaccaa agccgatcgt gtggacgaag cgcgtgttga tgaggttgaa
     3721 cgccaggtaa aggaggttct gcgggaatac ggttttgctg aggcaaaact gtttatcacc
     3781 gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa
     3841 cgcgagcacg ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa
     3901 ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca
     3961 ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag
     4021 ccaacagaaa ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa
     4081 aaagagcaga ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca
     4141 cgggtgattg tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt
     4201 caccacgccg ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa
     4261 ctggtcttcg acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc
     4321 tctgcccgca acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt
     4381 aaacgtaaac cagagtatct gcaatggctg gcttccctcg ccagggcaca gaatgatagc
     4441 gaagcgttag ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcctgggcg
     4501 cgccagctca acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct
     4561 ggttatagct tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca
     4621 ttagcgactt atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga
     4681 cgtatggcgt tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc
     4741 gaaagcggcg acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc
     4801 ttcagcgaag agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg
     4861 tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg
     4921 ctacgccagg cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt
     4981 aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca
     5041 acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt
     5101 ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc
     5161 gacgcattat tatttccgga aagataagga aatgattaat taatttttaa aataaattaa
     5221 tacaaatttc ttatgatttt aaataaagca cattgtttaa tgaatacaat gtgcttttta
     5281 ttagattaat tttgataatt aataataatg atcatgtttt cgaatgaaaa tcctcagtaa
     5341 gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt
     5401 ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat
     5461 tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt aggtatggcg
     5521 ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc
     5581 caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agaaaataat
     5641 tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg
     5701 ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa
     5761 ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt
     5821 ttctgcatca tcactgacga agcgcgtcat aacaaaatgg cgattgttga taagcacgtg
     5881 actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc
     5941 gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc
     6001 acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga aaacctgccg
     6061 ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc
     6121 cttgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag
     6181 ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttctgct gccgcatgtt
     6241 caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc tgcaatgggc
     6301 gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa cgccatccgt
     6361 gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa
     6421 gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac taatccgatc
     6481 caggcaactc acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc atcattccaa
     6541 caacggctgg caaattgtca gccgcttttt caactatctc tgtaatcctt gcccgtaaat
     6601 tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc
     6661 cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga
     6721 ttaagcccgg cgagtatggt ttccccatca agttaaaagc ccgctacgac aactttattg
     6781 gcggtgaatg ggtagcccct gccgatggtg agtattacca gaacctgacg ccggtgaccg
     6841 ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg
     6901 ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc
     6961 tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct
     7021 gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc
     7081 atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata
     7141 gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt
     7201 ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact
     7261 gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaattg
     7321 tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt ggggaaattg
     7381 gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag
     7441 tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg ctggagttgg
     7501 gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg
     7561 ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt tgcacctgtc
     7621 cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc
     7681 gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg
     7741 tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg
     7801 ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact
     7861 acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag gaggagattt
     7921 ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga
     7981 acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat ctggcctata
     8041 agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg
     8101 cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga
     8161 tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg
     8221 ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac
     8281 gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc
     8341 cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc
     8401 cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg
     8461 tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg aggccaatca
     8521 gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac
     8581 tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa acctcgtcct
     8641 gttgaaaaac cagacagcac atttcagaaa gtatataaaa aacaatatgt cgttatataa
     8701 ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata
     8761 ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt
     8821 ctggtgatat tctatgggat tattatttta catgatattc cctacctgat ttctaaaaaa
     8881 cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgctg
     8941 catgttatct ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg
     9001 ggaatgcaaa tccatgactc ctccgttgtg caactacaac agcgtattgc cgggctggag
     9061 aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta
     9121 ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata
     9181 aatggaccat ttccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt
     9241 taatgaacta taaccatccg tataccttca aggcgcaaaa agcggttatt tctattcctg
     9301 ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta
     9361 aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggtgcgg gtggatcggc
     9421 tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg
     9481 agatggcggc gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc
     9541 agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg
     9601 tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa
     9661 aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc
     9721 tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga
     9781 gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc
     9841 tacgtccggt gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc
     9901 agaactcctt gctgcgactg gcccctggcg acgatgcgga agtggtgttt aatgctctgc
     9961 caggtaaggt attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt
    10021 atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg
    10081 cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc
    10141 aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctattac
    10201 gcatgaccag ctgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt
    10261 gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat
    10321 ccagcacata ctggacccgc cagggcaggt attcccgttt acggaagata gccaccagat
    10381 ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc
    10441 tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg
    10501 ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac
    10561 tcccggcgac gttgaagcgc cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa
    10621 tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct
    10681 ggctggcata agcggttacg gcatattgtg taataaaact ggcaaccagc gattcatctt
    10741 ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa
    10801 aggcattacg ctcaa
//
LOCUS       DADLXX010000130        10698 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLXX010000130 DADLXX010000000
VERSION     DADLXX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.132"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            126..1823
                     /locus_tag="JNX27_003342"
     CDS             126..1823
                     /locus_tag="JNX27_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7884438.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="JNX27_003343"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="JNX27_003343"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY7884439.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="JNX27_003344"
     CDS             3113..3397
                     /locus_tag="JNX27_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7884440.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNX27_003345"
     CDS             complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNX27_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7884441.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4093..4977)
                     /gene="fdxH"
                     /locus_tag="JNX27_003346"
     CDS             complement(4093..4977)
                     /gene="fdxH"
                     /locus_tag="JNX27_003346"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7884442.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNX27_003347"
     CDS             complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNX27_003347"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7450..7452),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7884443.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8269..9150
                     /gene="yddG"
                     /locus_tag="JNX27_003348"
     CDS             8269..9150
                     /gene="yddG"
                     /locus_tag="JNX27_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7884444.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9409..10509
                     /gene="ompC"
                     /locus_tag="JNX27_003349"
     CDS             9409..10509
                     /gene="ompC"
                     /locus_tag="JNX27_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7884445.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2622 a         2743 c         2654 g         2679 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3481 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3541 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3601 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3661 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3721 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3781 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     3841 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     3901 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     3961 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4021 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4081 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4141 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4201 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4261 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4321 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4381 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4441 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4501 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4561 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4621 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4681 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4741 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga cgcagtgccc
     4801 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     4861 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     4921 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     4981 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5041 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5101 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5161 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5221 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5281 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5341 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5401 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5461 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5521 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5581 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5641 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5701 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5761 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     5821 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     5881 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     5941 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6001 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6061 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6121 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6181 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6241 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6301 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6361 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6421 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6481 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6541 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6601 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6661 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6721 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6781 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     6841 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     6901 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     6961 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7021 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7081 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7141 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7201 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7261 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7321 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7381 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7441 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7501 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7561 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7621 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7681 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     7741 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7801 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     7861 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     7921 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     7981 tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8041 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8101 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8161 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8221 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8281 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8341 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8401 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8461 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8521 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8581 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8641 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8701 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8761 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     8821 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     8881 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     8941 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9001 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9061 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9121 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9181 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9241 atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat
     9301 tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga cgccgtttaa
     9361 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
     9421 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
     9481 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
     9541 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
     9601 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
     9661 aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa
     9721 attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt
     9781 tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc
     9841 atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt
     9901 gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga caacagtcat
     9961 ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat
    10021 gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag
    10081 gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca
    10141 ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga aacattaaac
    10201 atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg
    10261 caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga
    10321 gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat
    10381 ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc
    10441 gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgctacggg cattacctat
    10501 cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat ttaagtgctg
    10561 ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat tcacctcaat
    10621 taatattatt ccatatcctc cggggacatc ttactataat atttattgat gagtgattat
    10681 gaccaatatt aatacagc
//
LOCUS       DADLXX010000131        10292 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLXX010000131 DADLXX010000000
VERSION     DADLXX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.133"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..401
                     /locus_tag="JNX27_003350"
                     /pseudo
     CDS             1..401
                     /locus_tag="JNX27_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="JNX27_003351"
     CDS             484..741
                     /locus_tag="JNX27_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884446.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNX27_003352"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNX27_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAY7884447.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNX27_003353"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNX27_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAY7884448.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNX27_003354"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNX27_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAY7884449.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNX27_003355"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNX27_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAY7884450.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNX27_003356"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNX27_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAY7884451.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNX27_003357"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNX27_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAY7884452.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /locus_tag="JNX27_003358"
     CDS             complement(8323..8844)
                     /locus_tag="JNX27_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY7884453.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9120..9324
                     /locus_tag="JNX27_003359"
                     /pseudo
     CDS             9120..9324
                     /locus_tag="JNX27_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9318..9422
                     /locus_tag="JNX27_003360"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="JNX27_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="JNX27_003361"
     CDS             9762..10019
                     /locus_tag="JNX27_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAY7884454.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2400 a         2733 c         2822 g         2337 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc ag
//
LOCUS       DADLXX010000132        10290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLXX010000132 DADLXX010000000
VERSION     DADLXX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.134"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..218)
                     /locus_tag="JNX27_003362"
     CDS             complement(<1..218)
                     /locus_tag="JNX27_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7884455.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRTLAEVY"
     gene            complement(399..1874)
                     /locus_tag="JNX27_003363"
     CDS             complement(399..1874)
                     /locus_tag="JNX27_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7884456.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKCFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3300)
                     /locus_tag="JNX27_003364"
                     /pseudo
     CDS             complement(2021..3300)
                     /locus_tag="JNX27_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="JNX27_003365"
     CDS             complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="JNX27_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7884457.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGITLAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5783..6529
                     /locus_tag="JNX27_003366"
     CDS             5783..6529
                     /locus_tag="JNX27_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7884458.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6619..7380
                     /locus_tag="JNX27_003367"
     CDS             6619..7380
                     /locus_tag="JNX27_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7884459.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7431..8315)
                     /locus_tag="JNX27_003368"
     CDS             complement(7431..8315)
                     /locus_tag="JNX27_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7884460.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="JNX27_003369"
     CDS             complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="JNX27_003369"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7884461.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9736..10149
                     /gene="msrB"
                     /locus_tag="JNX27_003370"
     CDS             9736..10149
                     /gene="msrB"
                     /locus_tag="JNX27_003370"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7884462.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10191..>10290
                     /locus_tag="JNX27_003371"
     CDS             10191..>10290
                     /locus_tag="JNX27_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HAY7884463.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVAL"
BASE COUNT         2721 a         2365 c         2490 g         2714 t
ORIGIN      
        1 cagtacacct cagcgagagt gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gagtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaac atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgatttt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggcct ggccctgacc
     3001 actgccggaa cctccgccgc caccctgggg acgttcaatt cggtcgttct gaacgaaatg
     3061 gtcattgccc ggatgcacgc ggtggcgcag accgccacgc ccctgatgaa acattggttc
     3121 gctaatatct tccgtgggaa tggatacgga ttcgctgctg tcgacgtcag tcaccgaacg
     3181 cttattaatg gcctcggaga tcgactgttt aatttgcgct ttataacggc gtaaaaaacg
     3241 ctggcgattc accatgcttt tgtttttgcc gttcagacgc cggtcaataa accaggtcat
     3301 atgccccccg tactacattt gccaactgta tgctacgcct gtgttgccgc ttctcaggcc
     3361 cacggttcgc tatcgttgta ggcccggtag catgtgtacc gggccagggt tgttaagacg
     3421 atttacgtac gcgcaaatac cattcgcaca gtaaacgcac ctgtttacgg gtgtagcctt
     3481 tttccatcat acggtcgaca aagtcgtcgt gtttcttctg ctcgtcggtt gacgttttgg
     3541 cgttaaacga gataaccggc aacagctcct cggtattgga gaacattttc ttctcgataa
     3601 ccgtgcgcag tttttcatag ctggtccagt tcggattgcg tccgctgtta ttcgctctgg
     3661 cgcgcagtac gaagttaaca atctcgttgc ggaaatcttt tggattactg atccccgccg
     3721 gtttctcgat tttctccagc tcggcgttaa gagactcgcg gtcaaacagc tgcccggtat
     3781 ccggatcgcg atactcctga tcctgaatcc agaaatccgc gtaggtaaca taacggtcga
     3841 aaatgttttg cccatattcg gaataggatt caaggtaggc cgtctggatc tctttgccga
     3901 taaactcggc atatttcggg atcagataac ctttcaggaa ctccagatag cgttctgcct
     3961 gctcttgcgg gaactgctcg cgctcgatct gctgttccag gacgtagaac agatggaccg
     4021 ggtttgccgc cacttctaca tgatcgaagt tgaacacgcg ggagaggatc ttaaacgcaa
     4081 aacgcgtcga cagaccgttc atcccttcat cgacaccggc gtagtcacga tattcctgat
     4141 acgacttggc tttcggatcg gtgtctttca gactttcgcc atcataaacc cgcatctttg
     4201 aataaatgct ggagttttct ggctctttca ggcgcgaaag aatggaaaaa cgtgacagtg
     4261 tttcgagcgt gccaggggcg catggggcgt gagtcaattc actgtgatta agcaatttct
     4321 cgtagatttt gatctcttcg gaaatgcgca agcaatacgg caccttcacg atgtaaacac
     4381 ggtcgaggaa ggcttcgttg tttttgttat tacggaaagt gacccattcg gactcgttcg
     4441 agtgggcgag agtaatcccg ttgaacggca gggcggagat accttccgtc ccgttgtagt
     4501 tatcttcctg agtggcggtt aacaagggat gcagcacttt tatcggtgct ttaaacatct
     4561 caacgaattc catgatcccc tgattggcgc ggcacagcgc accggaatag ccgtaggcgt
     4621 ccgggtcatt ctgcgcgtag tgttcgagtt tacgaatatc gactttccca accagcgcgg
     4681 agatgtcctg gttgttctca tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa
     4741 ttgacggcca gaccttcact acccggaact tagtgatatc gccaccaaat tcatgcaggc
     4801 gttttgccgc ccatggcgac atgatggtgc cgagataacg gcgaggaatg ccatactctt
     4861 tttccagaat ctgagcatct tcctgcggat tgaaaagaca gaacggatga tcgttgaccg
     4921 ggctacgctc accgttggcg ctcaatacat aaatcggtac gagctgcatt aatgatttca
     4981 gtcgctcagc aagcgatgat ttacccccac ccacaggccc cagcagatac aggatttgtt
     5041 tcttctcttc cagcccctga gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt
     5101 cttccatgcc gtaaaactct tcaaatgccg gataacgtgc aatgacccgg ttagaaaaga
     5161 gtcgagaaag tctgggttcc tgggctgtat cgaccatgac aggctcaccg atagccatca
     5221 atagccgctc agccgcgttg gcataagcac tgcgatcttg ccgacaagtg gtaagaaact
     5281 cctgcagtgt gaactcttcg tccttggcag cttcatatcg ctggcgatag tgatcgaata
     5341 tattcatggt atgccgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata
     5401 gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc
     5461 atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt
     5521 agatggcaaa tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac
     5581 tcatccataa attttgcata attaatgtaa agaccaggct cgccagtaac gctaaatcca
     5641 tttggctgta agcgcggtgt catccgcggc aggaaaatta aacagttact ttaaaaaatg
     5701 aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca
     5761 tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat
     5821 cgcaacgtct gcaggcgttg cgcacgctga aggtaaattt tccctgggcg caggcgtagg
     5881 tgtcgttgag cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa
     5941 ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga
     6001 cgcaacggat aaactttcaa ttaccgctta ctggtcgccg ctttacttca aagcgaaaga
     6061 cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct
     6121 gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg gcgataccct
     6181 ggataacagc aacggcattg tctgggatat ggcttggttg tatcgttaca ccaacggtgg
     6241 cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga acgaatacta
     6301 ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtgggtata acccgaatga
     6361 cagctggagc ccttaccttg agctgagcgc cagctacaac ttcctcggcg actggagtgt
     6421 ttacggtacc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc cgatggtgga
     6481 taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt
     6541 tccagggcat tttgtgcata aaaatagggc gtcacgccct gttttgcatt acggtggtgc
     6601 aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg
     6661 ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa
     6721 gttgctgcac tacgcgcagg cattgaactc ggtttaaccc tcattgatac cgccgaaatg
     6781 tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgagagaag
     6841 gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca
     6901 tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt attacactgg
     6961 tctggcagtt tcgcttttga agagactgtt gctgcgatgg aaaaattgat cgcccaggga
     7021 aaaatccgtc gctggggcgt ttctaacctt ggttatgccg atatgcagga actctggcag
     7081 ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg
     7141 attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt
     7201 ccattagtcc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa
     7261 attgcacatg tccaacaaaa tgcagctgcg cttgaggtcg aactttcgtc agcggaatta
     7321 gctatgctgg ataaggcata tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga
     7381 tgttgatacg tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg
     7441 gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt tggtgacttt ttgcgtttct
     7501 gaagcgtaag ccgtttctac acaaacaaag gtcttgtagc catcgtccgg catgtcgccc
     7561 atgctaactg aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg
     7621 cctacggcga taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc
     7681 agatacacgc gatcggtacg gtcagggaag gtctgaatac cgtcggtcag tacatcttct
     7741 ttcgcgtcat tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg
     7801 atatcaccca cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat
     7861 tcaagatcga tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc
     7921 ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca
     7981 gcatcttcat gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc
     8041 agaccttgtt gtgccgccgg accaaaccac ggccagcaaa ccggtacgct accgcgaata
     8101 gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca
     8161 ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga
     8221 tcgaccacaa tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt
     8281 tcgatgaccg gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta
     8341 tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt
     8401 atttggagat gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt
     8461 accaagatac cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca
     8521 cggaagtgca aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca
     8581 gaacgccttt catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag
     8641 ttgcagcttt ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc
     8701 ggaacgccat accagtcagt ttgccattca gttctggcag tactttacct acagctttag
     8761 cagcaccggt agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt
     8821 tgtgagacgg gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca
     8881 gaccttcgat gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg
     8941 tggtgcagga agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt
     9001 taacgaacat cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag
     9061 cggtgatgtg tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa
     9121 cgtcaacacc cacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga
     9181 tttttttacc gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc
     9241 cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga
     9301 ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac
     9361 ggccaaaacc gttgatacct actttgatag tcatatattc caccagctat ttgttagtga
     9421 ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa
     9481 tcattgcgac aaatcaatcc tgtgcctaag cattacgcgg ctgactcgcc tcactcttcc
     9541 tttgggcttg agaccacatg gggtcggcgc cccgaatttt aaagggcaat tacgataaaa
     9601 atgtgatttt catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa
     9661 ttttgccagt tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa
     9721 gtgagatgtg agtaaatggc gaataaacct tcggcagaag aactgaaaaa aaatttgtcc
     9781 gagatgcagt tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta
     9841 ctgcataaca agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat
     9901 tccgaaacca agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa
     9961 gaatccattc gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc
    10021 ggtaactgtg atgcccatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa
    10081 cgttattgtg ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc
    10141 aacggttgaa aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg
    10201 atgacattat caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac
    10261 tggggaaatg gcctgatggc gttgcgttga
//
LOCUS       DADLXX010000133        10205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLXX010000133 DADLXX010000000
VERSION     DADLXX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.135"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            31..834
                     /gene="hpaI"
                     /locus_tag="JNX27_003372"
     CDS             31..834
                     /gene="hpaI"
                     /locus_tag="JNX27_003372"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7884464.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            856..2211
                     /gene="hpaX"
                     /locus_tag="JNX27_003373"
     CDS             856..2211
                     /gene="hpaX"
                     /locus_tag="JNX27_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7884465.1"
                     /translation="MTTSSLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2221..3111
                     /gene="hpaA"
                     /locus_tag="JNX27_003374"
     CDS             2221..3111
                     /gene="hpaA"
                     /locus_tag="JNX27_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY7884466.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3219..4075
                     /locus_tag="JNX27_003375"
                     /pseudo
     CDS             3219..4075
                     /locus_tag="JNX27_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4302..5860
                     /gene="hpaB"
                     /locus_tag="JNX27_003376"
                     /pseudo
     CDS             4302..5860
                     /gene="hpaB"
                     /locus_tag="JNX27_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5878..6390
                     /locus_tag="JNX27_003377"
     CDS             5878..6390
                     /locus_tag="JNX27_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7884467.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6783..8933
                     /gene="btsT"
                     /locus_tag="JNX27_003378"
     CDS             6783..8933
                     /gene="btsT"
                     /locus_tag="JNX27_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7884468.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8983..9186
                     /locus_tag="JNX27_003379"
     CDS             8983..9186
                     /locus_tag="JNX27_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7884469.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9197..10153
                     /gene="yjiA"
                     /locus_tag="JNX27_003380"
     CDS             9197..10153
                     /gene="yjiA"
                     /locus_tag="JNX27_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7884470.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2428 a         2656 c         2643 g         2478 t
ORIGIN      
        1 ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa
       61 gcaggccgtc cgcagattgg attatggctg gggctgagca gcagctacag cgcggagtta
      121 ctggccggac caggattcga ctggttattg atcgacggtg agcacgcgcc gaataacgtg
      181 caaaccgtgc tcacccaact acaggcgatt gcgccctacc ccagccagcc agtggtacgc
      241 ccatcgtgga acgatccggt gcaaatcaaa caactgctgg acgtaggcac acaaacctta
      301 ctggtgccga tggtacaaaa cgccgacgaa gcccgtaaag cggtacgcgc cacccgttat
      361 ccccccgccg gtattcgcgg tgtgggcagt gcgctggctc gcgcctcgcg ctggaatcgc
      421 attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg
      481 cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggggtcga cggcgtgttt
      541 atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa
      601 gtacaggccg ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg
      661 atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc
      721 gccgtcggcg ttgacaccac cctgctcgcc cgcgccgccg aagcgctgac agcacggttt
      781 ggtaatagtt cttctatttc gatggcgaag caaaacaaca actctgtcta ctaaacgtac
      841 gatgtggagc gcatcatgac gacctctagc ctgcaatata aagataataa aactgttgaa
      901 attgaacatc gtgtcattaa taaattattt cgtcgcctaa tcatttttct ttttattctg
      961 tttgtttttt cgtttcttga tcgcatcaat ataggatttg ccggactgac aatgggcaag
     1021 gatcttggcc tgacgtccac aatgtttggc ctcgccgcga cactctttta tgtcacgtac
     1081 gtcatgtgcg ggatccccag taatatgatg ttagcgaaaa ttggcgctcg tcgatggatc
     1141 gctgggatca tggtggtctg gggaattgct tccacctgca caatgtttgc aacaagcccc
     1201 gaaacactct atctactgcg tatgctggtt ggtattgctg aggcgggttt tttacctggt
     1261 attttggttt atctgacctg gtggttcccg gcatatcatc gggccagagc caatgcctta
     1321 tttatgatag cgatgccagt cactatgatg ctgggattga tcctttcagg ctacattctg
     1381 gccttggatg gactgtggaa tctaaaaggc tggcaatggc tctttttgct ggaaggtctg
     1441 ccatcggtca ttcttggtgt cgtcacctgg ttttatctca acgacacacc agaccaggcg
     1501 acctggcttg atgaaaacga aaaacaggct ttaaaaacga tgattctccg tgaacaagag
     1561 agtgcatttg cccatgcatc tgttccacgc tcaaccttac gtgaagtcct gaccccagcc
     1621 atcatgctat atacacttgc ttatttttgc ctgactaata ctctgagtgc gattaatatc
     1681 tggacgccgc aaatcctaca gagtttcaat actggcagta gcaacatcat gattggtttg
     1741 ctggcggcca tcccccaatt ctgcacaatt tttggcatga tctggtggag tcgtcgttcc
     1801 gaccgtctta aagaacgcaa aaagcatacg attcttccct acctgtttgc cgctgccgga
     1861 tggttactcg cttctgcgac agatcacagc ctcattcaac tgagcggcat cattatggcc
     1921 tcaatgggat catttaccgc gatgactatc ttttggacaa cacaggatca agttataagc
     1981 ttgcaatcgc gtgccgttgc gatggctgtc atcaatgctg tcggcaatgt aggttcagca
     2041 gtcagtccat tacttatcgg tattttgaaa gatgcaaccg gcagttttag ctcaggtttg
     2101 tggtttgtag caggattgct ggtactgggc gcgttagtca tgacccgaat ccctatgggg
     2161 agtcgggaac actctctttc atctgaactt atcgcaccaa aaccacaata aggagtcatg
     2221 atgtccagtg aagctatctt tgaacatggt tatgaaaata ttgatttggt ccgggaatat
     2281 gatgcccgct acgctggtga agatgtgcat tatgaaacat ttacccggtt agcgaatttc
     2341 tttggacgcg atatgcgccc tcactggcac gatagatatt ttcaactcca ttatctggtc
     2401 actggacgca ttactttaca actggatgag cacttctatt ctgtgcgcgc accgcttttt
     2461 gtactaacac caccatcggt gccacatact ttctttactg atcctgatag cgatgggcat
     2521 gttctgaccg tacgtcagga gttaatttgg cccctggtag aaaagctctg gcctggtagt
     2581 ggagatgccg caatgctaca aggtatctgt ctgtcaccgg agggaatgga acatacactg
     2641 gcggcattaa atcattattg gccgataata tccctggagt ttcaacaaaa taagccagga
     2701 cgggaaattc tattgcaatc actggcgcaa agtattttta caatcctact gcgtgaagca
     2761 ccaccaaatg atatttcaac atgcagcgtg cgcggcgaga tgtgtttatt ccagaagttt
     2821 aatcggctga ttgacgaaaa ttatcgccaa catttaatgg tccctgaata tgcaacaatg
     2881 ctcggtatga gtgaatcacg gcttaccgag ctgtgccgac gttttgccaa tcaatcgccg
     2941 aagagattga ttttcgaacg agtgagccgg gaagccagac ggcttctgct ctatagcgag
     3001 caaagcatca accagatagc ccttgatctt ggctacaaag accccgccta tttcgcccgt
     3061 tttttcaacc gaatgatggg ttgctcacca acacaattcc gtggtcgctg accatttatc
     3121 aacctgagag actcgacatg aaaatatctt tacgctcact atttgtggcg tgcctgattg
     3181 cgccagtggc attcacctac gcctccgaac cgcaataaat gaaaattgta ctacttggaa
     3241 cgaaaggtgg cccttcttta ttaaaagctg accgtttacc gatggcaacg gcacttatta
     3301 ttggcaataa aacctggtta attgatgctg gttacggggc cagcttacaa ctggtaaaaa
     3361 ataatattcc actgcgccag attgataata ttttactcac ccatttacac agtgatcata
     3421 ttctggacta tccatcagtc ttaatgaatg catgggctag cggtctgcag gacaaaacta
     3481 ttcatgtatt cggtccgcca ggcacccgag ccatgaccgc tgccagttgg caggtcttcg
     3541 atcgggacat cacactgcgt atggaagaag aaggaaaacc cgatcctcgc aaactcgtta
     3601 cagctcaaga tgttggtgaa ggcgtagttt ataaagatga taccgtgacc gtgactgcat
     3661 taaaggtccc tcattctcct ttccctgacg gtgaggcttt tgcttatcgc ttcgacacac
     3721 aaggcaagcg aattgtcttc tcaggagata cctcgtactt tcccccactg gcaactttct
     3781 cagaaggcgc tgatatactt gtgcatgagg ccgtacatga gccctccgtc gctaagctgg
     3841 cagacagtat tggcaatggt aaaacactgg caaaagccat tgcttctcac catacgacta
     3901 ttgaggatgt aggcaaaatt gccaatgaag caaaagtgaa aaaactcatc cttagccacc
     3961 tggtaccaat caccgttccg gatgaaacat ggaagcaaga agcacagaaa actattcagg
     4021 ccctatcgtc gttggtcatg aaaatatgac gattaccatt ccattcgccc cataaaaaat
     4081 tccgtccccg ttagcccttg ttagcgggga atattatgat ttgccttact gtaagttgat
     4141 cacgatcgca gtttactatt taactccacc ttgctgtcga aaagtaccaa cgttcgcctg
     4201 aaccgtctct tcccgacaac cgcgttcaac gcttcaatta aataacaaaa acaacacaca
     4261 aaattaacaa tcgtattccg attaatactg tagaggtcga catgaaacca gaagatttcc
     4321 gcgccagtac ccaacgtcct ttcaccgggg aagagtatct gaaaagcctg caggatggtc
     4381 gcgagatcta tatctatggc gagcgagtga aagacgtcac cactcatccg gcatttcgta
     4441 atgcggcagc gtctgttgcc caactgtacg acgcgctgca caaaccggag atgcaggact
     4501 ctctgtgttg gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg
     4561 cgaaaagtgc cgacgacctg cgccagcaac gcgacgccat cgctgaatgg tcacgcctga
     4621 gctatggctg gatgggccgt accccagact acaaagccgc tttcggttgc gcactgggcg
     4681 cgaatccggg cttttacggt cagttcgagc agaacgcccg taactggtat acccgtattc
     4741 aggaaactgg cctctacttt aaccacgcga ttgttaaccc accgatcgtc atttgccgac
     4801 cgataaagtg aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt
     4861 cagcggtgcg aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt
     4921 cggctcggca caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat
     4981 ggatgccgat ggcgtgaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac
     5041 cggctcgcca tacgactacc cgctctccag ccgcttcgat gagaacgatg cgattctggt
     5101 gatggataac gtactgatcc catgggaaaa cgtgctgatc taccgcgatt ttgatcgctg
     5161 ccgtcgctgg acgatggaag gcggtttcgc ccgtatgtat ccgctgcaag cctgtgtgcg
     5221 cctggcagtg aaactcgact tcattacggc actgctgaaa aaatcactcg aatgtaccgg
     5281 caccctggag ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac
     5341 cttctgggca ttgagtgact cgatgtgttc agaagcgacg ccgtgggtca acggggctta
     5401 tttaccggat catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa
     5461 gatcaaaaac attatagaac gcaacgttac cagtggcctg atctacctcc cttccagtgc
     5521 ccgtgacctg aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa
     5581 cggtatggat cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag
     5641 cgaatttggt ggtcgtcacg agctgtatga aatcaactac tccggtagcc aggatgagat
     5701 tcgcctgcaa tgtctgcgcc aggcacaaag ctccggcaat atggacaaga tgatggcgat
     5761 ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa
     5821 caacgacgat atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg
     5881 caattagatg aacaacgcct gcgctttcat gacgcgatgg tcagcctgtc ggcagcggta
     5941 aatattatca ccaccgaggg cgacgccgga caatgcggga ttacggcaac ggctgtctgc
     6001 tcggtcacgg atacaccacc gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac
     6061 ccggtttttc agggcaacgg caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg
     6121 atggcgcgcc acttcgcggg catgacaggc atggcgatgg aagagcgttt tagcctctca
     6181 tgctggcaaa aaggtccgct ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa
     6241 ggagagagcc gcgatgtgca ggcgattggc acacatctgg tgtatctggt ggagattaaa
     6301 aacatcatcc tcagtgcaga aggtcacgga cttatttact ttaaacgccg tttccatccg
     6361 gtgatgctgg aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg
     6421 ggcattttta ttgtgatctc aatcacatat ctacccgatg aataattctt gcccaaccgc
     6481 tcaccgccat tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt
     6541 tacgcgcacg tatctctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac
     6601 gcaacgcaaa cctagaacgg cttcggccta tgattaacaa attactctat ggattttggt
     6661 ttgcaggaag gcagcaagtg agtgaatccc cgggagctta caaaagtaag tgtcagaggt
     6721 gaacgaacgc agctgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta
     6781 ctatggatac gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg
     6841 cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg
     6901 tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc
     6961 agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc
     7021 tgaactacgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg
     7081 gtgctggtcc gctggtggga ccggttctcg ccgcgcagat gggctacctg cctggcacgc
     7141 tgtggctgct ggcgggggta gtgctggccg gtgcggtaca ggactttatg gtgctgttta
     7201 tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag atggggccag
     7261 taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgttc
     7321 tggcgctgat tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct
     7381 caaccgtacc gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg
     7441 tgggtgaagt ctctgttatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg
     7501 tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct
     7561 tcgcgctgat tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac
     7621 cgcgcgacta tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta
     7681 tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg
     7741 gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg
     7801 tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa
     7861 ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg
     7921 cgctggttgc agcgtccatc atcgaaccgg gtctttactt cgcgatgaac accccgcctg
     7981 ctggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca
     8041 tcatggcgca gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcctggggct
     8101 tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc
     8161 tgaaccgtgc aggtggtgcg ccgacgctgg cggtaggtat cgctcacgtg ttccacaaag
     8221 tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt
     8281 tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc
     8341 tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta
     8401 ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg
     8461 gcggcgttaa gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag
     8521 cgctggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa
     8581 ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt
     8641 tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga
     8701 ttgctaacgg tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga
     8761 acaactacac caacgcaggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct
     8821 tctacggttt caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa
     8881 caccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt
     8941 tagccccgct tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga
     9001 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggtattcc agactacgac
     9061 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
     9121 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
     9181 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
     9241 gggcaaaact accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
     9301 gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
     9361 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggagga
     9421 cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg atcgtctggt
     9481 gattgaatgc accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga
     9541 gattttatgc cagcgctacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc
     9601 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gtgggctacg ccgaccgtat
     9661 tctgctgact aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac gcctggcgcg
     9721 catcaacgcc cgcgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct
     9781 gttcaacacc aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgtttcca
     9841 ctttatcgcg gataaacaaa acgatatttc gtcaattgtg gtggaactgg attacccggt
     9901 agatatcagc gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg cggataaact
     9961 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg
    10021 cgtccagcgc ctctacagcg ccgactggga caggccgtgg ggcgatgaaa aaccgcatag
    10081 cacaatggtg tttatcggga ttcagttgcc ggaggaagag attcgggcgg cgtttgcggg
    10141 attgaggaag taagttcggg aaagatcaac tttttgagag ttccaccgca acattattac
    10201 gagtg
//
LOCUS       DADLXX010000134        10073 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLXX010000134 DADLXX010000000
VERSION     DADLXX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.136"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(296..1156)
                     /locus_tag="JNX27_003381"
     CDS             complement(296..1156)
                     /locus_tag="JNX27_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAY7884471.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQPSTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKAGTPVVVGGGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQ
                     LPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNX27_003382"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNX27_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAY7884472.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JNX27_003383"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JNX27_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAY7884473.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATPERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JNX27_003384"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JNX27_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAY7884474.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JNX27_003385"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JNX27_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY7884475.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWFWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JNX27_003386"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JNX27_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..7965
                     /gene="lsrF"
                     /locus_tag="JNX27_003387"
     CDS             7090..7965
                     /gene="lsrF"
                     /locus_tag="JNX27_003387"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY7884476.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7989..8279
                     /gene="lsrG"
                     /locus_tag="JNX27_003388"
     CDS             7989..8279
                     /gene="lsrG"
                     /locus_tag="JNX27_003388"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY7884477.1"
                     /translation="MHVTLVEINVHEDKVEEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8318..9076
                     /gene="tam"
                     /locus_tag="JNX27_003389"
     CDS             8318..9076
                     /gene="tam"
                     /locus_tag="JNX27_003389"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY7884478.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9080..9994)
                     /locus_tag="JNX27_003390"
     CDS             complement(9080..9994)
                     /locus_tag="JNX27_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884479.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTYISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2431 a         2482 c         2653 g         2507 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt caagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgttctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 ggaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcgg aagataatgc caccccggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tagaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgc ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggt tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7201 tcacgcttat cgtggatatt taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac
     7261 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7321 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagttccc
     7381 ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa
     7441 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     7501 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     7561 cagctggtcg atgccggaat gaaagtggga atgccgacca tggccgtgac tggcgtgggc
     7621 aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg
     7681 ggggcgcaaa ttatcaaaac ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     7741 tgtccggtac ccattgttat tgctggcggt aaaaaattac cggagcgcga ggcgctggaa
     7801 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     7861 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     7921 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     7981 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgaagagt
     8041 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggt aatttgcgct
     8101 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatttacgaa gcctataaag
     8161 atgaagacac agtggcattc cataaaacca cgcctcacta caaaacctgt gtcgcgaaac
     8221 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8281 gctacaattg gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt
     8341 atatctgcac ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc
     8401 gctggagaat gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct
     8461 gctgcatcaa cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat
     8521 tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg
     8581 gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga
     8641 ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg tgctggcagt
     8701 acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcttggga
     8761 acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat
     8821 tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc
     8881 gtcgcaccag gcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga
     8941 tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgc tggaagagca
     9001 gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc
     9061 ccgccgtacg gagtaattat cacgtcagct ggtaatgacg atcgggaaga atttttgctg
     9121 gaatttctgc ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat
     9181 ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat
     9241 ccagcgaaat ataagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt
     9301 ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca
     9361 gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg
     9421 aaatatcatt aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgtccattgc
     9481 ggcgctgaac cgccaaccat tctcccatta ttaacaagat acggttagct ggagagttcg
     9541 aagccagtac acgctgaaga tctccagtct tgagataatg agccagcact tccgcctgtg
     9601 gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact
     9661 cccttacact tgccgaatcc ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg
     9721 caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat
     9781 tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga
     9841 atttgatgcc cagatgcgtg taccagggca gcatgaatat aacagcaata gaaaagagaa
     9901 aattgagtag taagcgcgag gatatcttgg ataatactga gccgtgccag acaaaaatac
     9961 ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg
    10021 ccctattaaa cgtgattacg atcacattct caagacgcta cttacaaatt acc
//
LOCUS       DADLXX010000135         9990 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLXX010000135 DADLXX010000000
VERSION     DADLXX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.137"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..338)
                     /locus_tag="JNX27_003391"
     CDS             complement(<1..338)
                     /locus_tag="JNX27_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7884480.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="JNX27_003392"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="JNX27_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="JNX27_003393"
     CDS             complement(1132..1338)
                     /locus_tag="JNX27_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7884481.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="JNX27_003394"
     CDS             complement(1445..2008)
                     /locus_tag="JNX27_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7884482.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="JNX27_003395"
     CDS             complement(3383..5218)
                     /locus_tag="JNX27_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884483.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="JNX27_003396"
     CDS             5319..5606
                     /locus_tag="JNX27_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7884484.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="JNX27_003397"
     CDS             5578..7107
                     /locus_tag="JNX27_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY7884485.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="JNX27_003398"
     CDS             complement(7277..8494)
                     /locus_tag="JNX27_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7884486.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="JNX27_003399"
     CDS             complement(8861..9847)
                     /locus_tag="JNX27_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884487.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         3144 a         2271 c         1856 g         2719 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg cggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagataga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg taccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcaacg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacaaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 taggcagcct gacggctgcg gcttgtcatg
//
LOCUS       DADLXX010000136         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLXX010000136 DADLXX010000000
VERSION     DADLXX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.138"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNX27_003400"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNX27_003400"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7884488.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNX27_003401"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNX27_003401"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7884489.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JNX27_003402"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JNX27_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JNX27_003403"
     CDS             3456..5216
                     /locus_tag="JNX27_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY7884490.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JNX27_003404"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JNX27_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7884491.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JNX27_003405"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JNX27_003405"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7884492.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRISTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JNX27_003406"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JNX27_003406"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7884493.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2551 a         2495 c         2377 g         2532 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccaag
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttgggt aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt atctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADLXX010000137         9950 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLXX010000137 DADLXX010000000
VERSION     DADLXX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.139"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..221)
                     /locus_tag="JNX27_003407"
     CDS             complement(<1..221)
                     /locus_tag="JNX27_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7884494.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKA"
     gene            653..1771
                     /locus_tag="JNX27_003408"
     CDS             653..1771
                     /locus_tag="JNX27_003408"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7884495.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1837..2085
                     /locus_tag="JNX27_003409"
     CDS             1837..2085
                     /locus_tag="JNX27_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7884496.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2150..2518
                     /locus_tag="JNX27_003410"
     CDS             2150..2518
                     /locus_tag="JNX27_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7884497.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2612..3265
                     /gene="nfsB"
                     /locus_tag="JNX27_003411"
     CDS             2612..3265
                     /gene="nfsB"
                     /locus_tag="JNX27_003411"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7884498.1"
                     /translation="MNIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3373..4620
                     /locus_tag="JNX27_003412"
     CDS             3373..4620
                     /locus_tag="JNX27_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7884499.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTKAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4688..6064)
                     /gene="pheP"
                     /locus_tag="JNX27_003413"
     CDS             complement(4688..6064)
                     /gene="pheP"
                     /locus_tag="JNX27_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7884500.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGDAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6165..9300)
                     /gene="cusA"
                     /locus_tag="JNX27_003414"
                     /pseudo
     CDS             complement(6165..9300)
                     /gene="cusA"
                     /locus_tag="JNX27_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9312..9853)
                     /locus_tag="JNX27_003415"
                     /pseudo
     CDS             complement(9312..9853)
                     /locus_tag="JNX27_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2444 a         2745 c         2401 g         2360 t
ORIGIN      
        1 gctttcacga ccttaatacg caactctgga tcagcactaa cgcctttagg tttgggaatt
       61 aaaccttttt ctccatgttt ttcatagagg gcaacccatg tcctgacctg ggttcggggg
      121 acaccaaaac gtgccgagat gatcctgtaa ccatcatcag tagtgaagta gtgattcacg
      181 acttcaaggc gcttttcaaa agggtatttt ggctttgaca tattaggggc tattccattt
      241 catcgtccaa caaaatgggt gcagtacagg gttgatagaa atatctcctg gggcttcatc
      301 tttccagacc tcagagtaaa cctgaaatcg gaaagcttca ggcacccgca gttatcttac
      361 ccttcctcaa caaaatagct gtttatttat acagcaaatc ctgtttcgga cttctcactc
      421 cccatttgat caactgaagc tgaacccgtt tttttctgca tgcctcaatc gtcaaaattc
      481 ttcgccacca gccagctctg ccataacttg ccacattgaa aaagtagcca aagtgcttca
      541 ggcaccatca gttcaccatc accaaaaaaa agaaacattc cagaacttcc gcatctcgcc
      601 cagccgctcc gacttctata ctgaatagaa aacgtcaact taagagaaac ctatgccatt
      661 acccgatttt catttttctg aaccttttac cctcggtatt gaactggaaa tgcaggtggt
      721 taatccgccg ggctatgact taagccagga ctcttcaatg ctgattgacg cggttaaaaa
      781 taagatcacg gccggagagg taaagcacga tatcaccgaa agtatgctgg agctggcgac
      841 ggatgtttgc cgtgatatca accaggctgc cgggcagttt tcagcgatgc agaaagtcgt
      901 attgcaggca gccgcagacc atcatctgga aatttgcggc ggtggcacgc ccccgtttca
      961 gaaatggcag cgtcaggagg tatgcgacaa cgaacgctat caacgaacgc tggaaaactt
     1021 tggctatctc atccagcagg cgaccgtttt tggtcagcat gtccatgttg gctgtgccag
     1081 tggcgatgac gccatttatt tgctgcacgg cttgtcacgg tttgtgccgc attttattgc
     1141 cctttccgcc gcgtcgccat atatgcaggg aacggatacg cgttttgccc cctcacgacc
     1201 gaatattttt tccgcctttc ctgataatgg cccgatgccg tgggtcagta actggcaaca
     1261 atttgaagcc ctgtttcgct gtctgagtta caccacgatg atcgacagca ttaaagatct
     1321 gcactgggat attcgcccca gtcctcattt tggcacggtg gaggttcggg tgatggatac
     1381 cccgttaacc cttagccacg cggtgaatat ggcgggatta attcaggcca ccgcccactg
     1441 gttactgacg gaacgcccgt tcaaacatca ggagaaagat tacctgctgt ataaattcaa
     1501 ccgtttccag gcctgtcgct atggtctgga aggcgtcatt accgatccgc acactggcga
     1561 tcgtcgaccg ctaacggaag ataccttgcg attgctggaa aaaatcgccc cttcagcaca
     1621 taaaattggc gcatcgagcg caattgaagc actgcatcgc caggtcgtca gcggtctgaa
     1681 tgaagcgcag ctgatgcgcg atttcgtcgc cgatggcggc tcgctgattg ggctggtgaa
     1741 aaagcattgt gagatctggg ccggtgacta agccgggaat tgccttacag aagaatatcc
     1801 ctgacaatgg tgttttaact tacatttaac aatgttatga aacacccttt agaaaccttg
     1861 accaccgcag caggcatttt gctgatggct ttcctctctt gcctgctgct gcccgccccc
     1921 gcactggggc tgacgctggc acaaaaactg gtgaccatgt tccatctgat ggatcttagc
     1981 cagctttaca ctttattgtt ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt
     2041 gttctgcgct ttatctggcg acgctggttc tcgctggcgg attaaacgtc gattatcgcc
     2101 gaacttcgcg gcatactttg cttattctcc ttcgcctaaa ggaacgttta tggataagca
     2161 atcactgcac gaaacggcga aacgcctagc tcttgagtta ccctttgtcg agctttgctg
     2221 gccttttggc ccggagttcg atgtttttaa aattggcggc aagattttta tgctgtcgtc
     2281 ggagctacgc ggcgtcccct ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa
     2341 tcagcaaata tacccaagca ttaagccagg gtatcacatg aataaaaagc actggatttc
     2401 ggtgtattcc ggcgaggaaa tctccgaagc gttacttcgc gatctgatca acgattcgtg
     2461 gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag
     2521 cgggaatcta tagcgcattt ttctcgctta ccatttctcg ttgaaccttg taatctgctg
     2581 gcacgcaaaa ttacttacac atggagtctt tatgaatatc atttctgtcg ccttaaagcg
     2641 tcattccact aaggcatttg atgccagcaa aaaacttacc ccggaacagg ccgagcagat
     2701 caaaacgcta ctgcaataca gcccatccag caccaactcc cagccgtggc attttattgt
     2761 tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc gctgccggta attatgtgtt
     2821 caacaaacgt aaaatacttg atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat
     2881 ggacgatgcc tggctgaagc tggttgttga ccaggaagat gccgatggcc gctttgccac
     2941 gccggaagcg aaagccgcga acgataaagg tcgcaagttc ttcgccgata tgcaccgtaa
     3001 agatctgcat gatgatgcag agtggatggc aaaacaggtt tatctcaacg tcggtaactt
     3061 cctgctcggc gtggcggcgc tgggtctgga cgcggtgccc atcgaaggtt ttgacgccgc
     3121 aatcctcgat gcagaatttg gtctgaaaga gaaaggctac accagtctgg tggttgttcc
     3181 ggtaggtcat cacagcgttg aagattttaa cgctacgctg ccgaaatctc gtctgccgca
     3241 aaacaccacc ttaaccgaag tgtaattctc tcttgccggg catctgcccg gctatttccc
     3301 ctcagattct cctgatttgc ataaccctgt ttcagccgtc atcataggct gctgttgtat
     3361 aaaggagacg ttatgcagga tttaatatcc caggttgaag atttagcggg tattgagatc
     3421 gatcacacca cctcgatggt gatgattttc ggtattattt ttctgaccgc cgtcgtggtg
     3481 catattattt tgcattgggt ggtactgcgg accttcgaaa aacgtgccat cgccagttca
     3541 cggctttggt tgcaaatcat tacccagaat aaactcttcc accgtttagc ttttaccctg
     3601 caggggatta tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac caaagcggca
     3661 gatattctga ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc
     3721 tcgttgctgg atgttatttt gaatctggcg cagaaattcc cggcggcatc tcagttaccg
     3781 ctgaaaggga tatttcaggg gattaaactg atcggcgcaa tcctggtcgg cattttgatg
     3841 atctcgctgc tgattggtca gtcgccagcg attctgatca gcggtcttgg tgcaatggct
     3901 gccgtgctga tgttggtatt taaagatccg attcttggtc tggtggcagg tattcagctt
     3961 tccgcgaacg atatgctgaa actgggcgac tggctggaaa tgccgaaata cggcgcggat
     4021 ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc gtaactggga caataccatt
     4081 accactattc ccacctggtc tctggtttct gactccttta aaaactggag cgggatgtca
     4141 gcatctggcg ggcgacgtat taagcgcagt atcagtattg atgtcaccag tattcgtttt
     4201 cttgatgaag acgaaatgca acgtctgaat aaagcgcatt tgttaaagcc ttatttaacc
     4261 agccgccatc aggaaattaa tgagtggaat cgccagcaag gttctacgga gtcggtatta
     4321 aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct atctgaacga atatctgcgt
     4381 aaccatccgc ggattcgtaa agatatgacc ttaatggtac gccaactggc tccaggtgat
     4441 aacggtttac cgctcgagat ctacgcgttt actaacaccg tggtgtggct ggaatatgaa
     4501 agcattcagg ctgatatatt cgatcacata tttgcgattg tcgaagagtt tggtctgcga
     4561 cttcatcagt cgccaaccgg caatgatatt cgctctctgg cgggtgcatt taagcagtaa
     4621 ttaaaaaaac cgctctcatc gaatggatga gagcggacat cgttttgtcg gatgtagcgt
     4681 gaatgcctta tttccgacgc agcgttttaa atgccacaaa caggaataca atccacaccg
     4741 gcagcaggat cgctgacaag cgcatatcat ccatcgtgca catcagcagc aaaatcatgc
     4801 cgaggaaggc gatgcaaaga tagttgccga acggatagag cagcgcctta aactgtgttt
     4861 cacgcccctg acgtcgcatc gccgcgcgaa aacgcagatg cgccaggcag atcataatcc
     4921 agttcaacag caacgttgct accaccagcg tcatcagcag accaaacgct ttttgcggca
     4981 gcagatagtt gattaacacc accagcgaag tgatcgctcc ggaaagcatc agcgagttaa
     5041 tcggcacacc gcgacggctg acgcgagtca aaaacttcgg cgcattaccc tgcacagaaa
     5101 ggccaaacag catgcggctg ttagagtaaa ccccgctgtt atacaccgac agcgatgcca
     5161 ccagaatgac gaagttcagc gcagaagcta ccacattgct gtcgagatta tggaaaatca
     5221 tcacaaacgg gctactgttg gatttcactt ccacccacgg atagagtgcc agtaaaacca
     5281 ccagtgaacc gatataaaac agcaggattc gatacaccac ctgatttacc gcttttggaa
     5341 tgcttttttc cggatcgcgc gcttcagcgg ctgtaatccc aatcagctcc agaccgccga
     5401 aggagaacat aattaccgcc agcgacaaaa tcagcccatt ccggccggtg gcgaagaaac
     5461 caccgtagcg ccagaggttg tcgatactgg ctttctcgcc gccgtgacca gaaaacagca
     5521 gccacaggcc aaagccgatc ataccgatga tcgccagcac tttaatcagc gcaaaccaga
     5581 actcggtttc gccatataag cgcacgttca ccaggttaac ggcgttgatg ataataaaga
     5641 aggcggcagc ccaaatccac gttggaacat ccgggaacca gtactgcata tagatgcccg
     5701 cagcggtcag ctctgccatt cccaccagca cgaacattac ccagtagttc cagccagaga
     5761 ggaagcccgc aaacggtccc cagtatttat aggcaaagtg ggcaaatgaa ccggataccg
     5821 gctcttcgac caccatctcg ccaagctggc gcataatcag gaaagcgatg atcccggcga
     5881 cgccgtagcc cagcaataca gccggacccg ccatctgaat cgccgggcca atgccaagaa
     5941 acagaccagt accaattgcg tcacccagcg caatcagttg aatatgacgg ttatgtaatc
     6001 cgcgatgaag cgtcggctct tgattcgacg cagtatcttc cgatacggtt gacgcgtttt
     6061 tcacgccttt cccctgtgtg tcttttttgt tgaggggcac cttttaacat ttagtgccca
     6121 tcgtcgcaag acacaatcca cacggttaaa cgaggtatcc tgctttaatt ccgtacccga
     6181 tgtcggtgca gccacatcag cttatacgcc gccgggataa taaacagcga cagcaaaggt
     6241 gcggtgatca tgccgccaat catcggtgcg gcaatccggc tcatcacctc tgaaccagcc
     6301 cccgttcccc acagaatcgg cagcagaccg gcgataatca ccgccaccgt catcgctttc
     6361 gggcgcacgc gcaggaccgc gccgtgatac aacgcctcat ccagcttctg ctcgctgaat
     6421 gtttgtggat tattcaacga cggctcggcc tctatggcgt gacgtaaata catcagcatc
     6481 accacgccaa attcggcggc gaccccggca agggcaataa agccggtacc cgtcgccaca
     6541 gaaagatgaa agcccatcca ccacagtaac cagatgccgc ccaccagcgc aaacggtacg
     6601 ctgctgataa tcagcaacgc ttcgcccacc cgacggaatg ccagatacaa cagcacgaag
     6661 atgatcatca gtgtcatcgg caccatgagt ttcagcttat ggttggcgcg ctccagtagc
     6721 tcgaactgcc cggagaatgc cacgctggtg cccggtttta actgcacttt ttcagctatc
     6781 gctttttgca aatcgtgaac caccgacacc atgtcacgat cgcgggcatc gatataaatc
     6841 cagctcgtcg ggcgcgcatt ctcggttttc agcatcgacg gtccggtaga gactttaacg
     6901 tcggccacgt ctgccagggt gatttgctgc ttcatcggcg tcaggatcgg caactggcgc
     6961 agtgcctgcg gactatcgcg ccagctttgc ggataacgca gattaattgg ataacgggca
     7021 atcccttcca ccgtttcgcc aaccatcgcc ccgcccaccg cagaagtcac aaacaactgc
     7081 acatccgcca ccgtcatacc gtaacgtgcg gctttttcac ggttaatctc aacgttgata
     7141 tagcgcccac cttccagccg ctcggcaagc gcagaagcta cgcctggcac cgttcgcgcc
     7201 acttcttcaa tttgctcagc catcgcgtcg atatccgcca gcacagtgcc ggaaacttta
     7261 atgccgatgg ggcttttaat gccggttgag agcatatcga tacggttacg aattggcggc
     7321 acccacagat tcgccagccc cggcagacgc acggtgttat ccagttcctc aatgattttg
     7381 tccattgtca tgcctggccg ccactgatcc tgtggcttaa gctggatggt cgtttctacc
     7441 atctccagcg gagcagaatc ggtggcggtt tccgctttcc cggttttgcc aaatacccgc
     7501 gccacttcag gtacgctcat aattagcttg tcggtttttt gcagcatact cgccgcctct
     7561 gctgcggaaa tccccggcag cgtcgatggc atatacaaca agtcgccttc attgatctgc
     7621 ggtaaaaatt ccccgccaac tttattgagc ggccagagaa ccgtcagcac cgaaagcgcc
     7681 gccaccagca gcgtggtttt cggccagtgc agtactttca gcaacagcgg atgataaaca
     7741 cgaatcaaaa agcgattgag cggattactg ctttccggcg gaattttgcc acggatccag
     7801 taacccatca ggatagggat cactacgatc gccagcagcg ccgcacccgc catcgcatac
     7861 gttttggtga acgccaacgg gccaaacaga cgcccttcct gcccttccag ggtgaagatc
     7921 gggataaacg acaacgtgat aatcagcaga ctgataaaca gcgccggccc cacttcaaca
     7981 gacgcatcgg tgatcacctg ccagcgcgtt ttattatcca gcgtggcgtc aggatgctgg
     8041 tgctgccact cttccagccg tttatgcgca ttctcgatca tgacgatagc ggcatcgacc
     8101 atcgccccga cggcaatcgc aatgccaccc agcgacataa tattggcatt cagcccctgg
     8161 aagggcatga caataaaagc aatacacaac cccagcggca acgaaataat cgccaccagc
     8221 gccgagcgca catgccagag aaacagcgcg cagactaccg ccaccacaat aaactcttcc
     8281 agcaacttgc cgctgaggtt gtcgatggcc cggtcaatga gctggctgcg gtcgtatgtt
     8341 gtaactatct ccacgccttc cggcagacta cttttcagcg tttccagttt gtccttcacg
     8401 gcggcgatca cttctcgggc gtttttgccg gatcgcagga tcaccacccc gcccgccact
     8461 tcgccttcgc cgtttaatgc cccggcgcat ctccgggcca atctggtcct tcgcaacatc
     8521 gcgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc
     8581 gagcgtttgt agatagccgc tggcgcgcac catatattct gcttccgcca gttcgattga
     8641 cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg cgagactgat
     8701 gccatactgc gccaggcgct gaggatcgat aaccacctga tactctttca ccacaccgcc
     8761 taccgacgcc acttccgcaa cgtcaggaat ggttttcagc tcatatttga gaaaccagtc
     8821 ctgcaatgag cgtaaatcgg caagatcgtg cttaccgctg cgatccacca gtgcatattc
     8881 atagatccag ccaacacccg tggcatctgg tcccagctcg gcgctgactc ccgcaggcag
     8941 cttaccctgt acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt
     9001 gccatcttcg aaaatgacat acacataaga gtcgccaaac tgcgagaaac cacgcacagt
     9061 cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac
     9121 gatttgcggt gcctggccgg gatagctggt tttaataatc acctgcacat cggagagatc
     9181 cggcaacgca tccactggcg tattaatgat ggtccaggtg ccccagatgc tcagaaacaa
     9241 cgcgcccatc agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat
     9301 tggttattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca
     9361 gaaatattgg cttcggaatc aatcaggaac aggccgccgg aaaccacctt ttcaccttcc
     9421 gccagaccag agcgtaatgc ggtgacgcct tgcgatgcct ggaaaacagc aacgcgtttc
     9481 ggtacaaagc gcccgtcggc atcaacggta atcacccgct gttcgttgcc ggtatcaatc
     9541 agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt cgcggcatcc
     9601 acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg cgccggaacg
     9661 gtgagggtaa actgcgaggc atctttcacc agccaggcga tggactccgg gatcgcagca
     9721 gtgacccaca ccgggtccat accctgaatt cgcgattacg cgcaataccc ctgagaaccg
     9781 ccatgaacaa gggtttcatg atttgtgatt ttagtttagt acgcaatttg gtacacaaca
     9841 ctattttcac ttcagcgggt agtcatcaaa ctcaccataa cgagcatcgt tgatgatgta
     9901 tgtgatcacc acaaatatca catctggact agttccgtct tcatgcgcag
//
LOCUS       DADLXX010000138         9718 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLXX010000138 DADLXX010000000
VERSION     DADLXX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.140"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..483
                     /locus_tag="JNX27_003416"
     CDS             <1..483
                     /locus_tag="JNX27_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7884501.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
                     "
     gene            639..2174
                     /gene="gadC"
                     /locus_tag="JNX27_003417"
     CDS             639..2174
                     /gene="gadC"
                     /locus_tag="JNX27_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7884502.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2305..3624
                     /locus_tag="JNX27_003418"
     CDS             2305..3624
                     /locus_tag="JNX27_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7884503.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3989..5134
                     /locus_tag="JNX27_003419"
     CDS             3989..5134
                     /locus_tag="JNX27_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7884504.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            5059..5676
                     /locus_tag="JNX27_003420"
                     /pseudo
     CDS             5059..5676
                     /locus_tag="JNX27_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5934..6266
                     /gene="ddpX"
                     /locus_tag="JNX27_003421"
                     /pseudo
     CDS             5934..6266
                     /gene="ddpX"
                     /locus_tag="JNX27_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6280..7176
                     /locus_tag="JNX27_003422"
     CDS             6280..7176
                     /locus_tag="JNX27_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7884505.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7173..8159
                     /locus_tag="JNX27_003423"
     CDS             7173..8159
                     /locus_tag="JNX27_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7884506.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            8152..9078
                     /locus_tag="JNX27_003424"
                     /pseudo
     CDS             8152..9078
                     /locus_tag="JNX27_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9134..9565)
                     /gene="osmC"
                     /locus_tag="JNX27_003425"
     CDS             complement(9134..9565)
                     /gene="osmC"
                     /locus_tag="JNX27_003425"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7884507.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2359 a         2385 c         2451 g         2523 t
ORIGIN      
        1 ggtggtcaga ttggtacttt tgccatcaac ttctcccgcc cggcgggtca ggtgattgca
       61 cagtactatg aattcctgcg ccttggtcgt gaaggctata ccaaagtaca gaatgcttcc
      121 taccaggtcg ctgcctatct ggcggatgag atcgccaaac tggggccgta tgagttcatc
      181 tgtaccggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      241 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      301 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      361 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      421 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      481 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggata atttccaaag
      541 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      601 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      661 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      721 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      781 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      841 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggt ccgagatggg
      901 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      961 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat gaagacccca
     1021 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
     1081 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1141 catttatttt gatcgcatta gcggctattt acctgcactc cggtgccccc gttgctatcg
     1201 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcacactg gttgtatttg
     1261 ttgccttcat tttgagttat atgggcgtag aagcttccgc aactcacgtc aatgaaatga
     1321 gcaaccccgg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1381 taagctctgt tggcggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1441 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1501 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1561 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1621 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1681 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1741 tgatcgcact ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1801 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1861 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1921 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1981 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     2041 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     2101 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2161 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa ttcatcgctc
     2221 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2281 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2341 agaagaccag cgatactggt tgcattggca cttttactgt gtagttgtaa aagcacgcct
     2401 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2461 tcacaaccga tgcgtggcat ctggctggcc acggtttctc gcctcgactg gccaccggtt
     2521 tcctcggtta acattagtaa tcccaccagc cgagcccgtg tacaacaaca ggcgatgatc
     2581 gacaaactgg atcatctcca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2641 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2701 attggtgaaa atccgggtta cgatccgctg caattcatgc tcgacgaagc ccacaagcgt
     2761 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2821 actatcaggg aactgaatag cactctgtct caacaaccgg cgagcgtcta tgtgcaacac
     2881 cgtgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2941 caggactgga tcacatcaat agtcgctgaa gtggtttccc gctatccggt agatggcgtg
     3001 cagtttgacg actatttcta tactgaatca ccgggttcac ggctaaatga taacgaaacg
     3061 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3121 cagttaattg caaaggtatc acacaccatt aaaagcatta agccgggagt cgaatttggc
     3181 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3241 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3301 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3361 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3421 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3481 ggcgtaccgg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3541 accatcttgt tccgtgagga ctatctgaat aaaccgcaga ctcaacaagc ggtcagctat
     3601 ctgcaaagtc gttggggcag ttaaactacc atgcttactt tgtaacaagc cggtgcttta
     3661 cccaccggct tgttacacct tgtgaaatat cccactagta atcatgctta cataagtcaa
     3721 attaaccaca cttagttaaa cagctataaa tccctaataa aaatgtatga atatgaaaaa
     3781 ccatacgata ttcgctgtca ccgaaaaata attacgatgg gctatactct tctgaagaac
     3841 agtagcgtag attgaaactg tcttaagtcg cttatttctt ttcagtatat cttcatattt
     3901 caggagaata tggtgcagtt agtcgatctc gcacgttgtg tttcgttttg attccgtctg
     3961 atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa tgaaagatga
     4021 gtggaccgga cttgtcgaac aggcagatcc tctcattcgt gctaaagccg cagaaattgc
     4081 ccttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca tcgacccgca
     4141 tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg cgatggaacg
     4201 ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa tacaaatcca
     4261 gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg tcgagatggg
     4321 gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt attccgccgc
     4381 agaaaaactt caggtctacc atttctcgat taacagtatt gatatcgcga tggaagtgat
     4441 gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa actatcgtat
     4501 cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct caatactttc
     4561 atgggaaata gatattatct ataaagtcct gctggattct gatttaggca gcagtttgcc
     4621 tttaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt attttagcgg
     4681 cattgccgaa gtgggccata tctcccgtct gattcaggat ttcgacggta ttttcaatca
     4741 aaccatgcgt aacaccagga ttttgaataa cagaagtttg cgggtgaaat ttctattaca
     4801 gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg aagaagtatc
     4861 gcgccacgaa gtcggtatgg atgtactgac gaaattactt aaccgccgtt tcctgccgac
     4921 tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt cagtgctgat
     4981 tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca ctggcgatga
     5041 aattctgcgt aaagtctcat tcctctcgca gaagagattg gtgaaatcga aaatattggg
     5101 cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat
     5161 atcccggcgt tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat
     5221 caggtgtctg atgcaatgca agcctggggt attgacggcc accagctgac ggtggaaatc
     5281 acggaaagca tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgt
     5341 gatatgggcg tgggtttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc
     5401 ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa gctttgtcga tcgttgtctg
     5461 acggaaaaac gcatcctggc cttacttgaa gccattacca gcattgggca aagcctcaat
     5521 ttaaccgtcg tggcggaagg ggtcgaaacc aaagagcaat ttgagatgct acgtaagatc
     5581 cactgtcgcg ttattcaggg atatttcttt tcccgccccc ttcccgccga agaaattcca
     5641 ggctggatga gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc
     5701 gggttttcct ttaaggtgac actgtcactt aaatactgtg atttcagcta cgattccggg
     5761 agattccttc cttaactttc cctcttgcac caaaactgca ctacgctgca caaatgttga
     5821 tcaaaagtta aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg
     5881 agatctgcat aagcggaaag cgcagcaatt tttgtcttat acaggaaccg tttatgtcgg
     5941 ataccaccga actggttgat ttagccgtga tcttccctga tctggagatc gaattgaaat
     6001 atgcctgcgt tgataacatc acaggtaaag ctatttatca gcaagcgcgt tgtctgctac
     6061 ataaagatgc gattaccgcg ctggcgaaaa gtatcagtat cgcccagctg tcaggcttac
     6121 aactggtgat ttacgatgcg tatcgcccac aacaagcaca ggcgatgttg tggcaagcct
     6181 gcccagaccc gcaatatgtt gttgatgtga cggtcggttc taatcacagc cgtggcacgg
     6241 cgctcgacct gacgcttcgt gatgacgtgg aggtggtgaa tgatgctaag cgaggaaacg
     6301 tccgccgtac gcccacaaaa acaaacgcga tttaacggtg caaaactggt atggatgctg
     6361 aaaggcagtc cgctcaccgt gaccggcgca gtcatcattg tattaatgcg actgatgatg
     6421 attttttcac cgtggctggc gacgcatgat cccaacgcca ttgatttaac cgcccgcctt
     6481 ttgccgcctt ctgcggcgca ctggtttggc accgatgaag tgggacgcga tctgtttagc
     6541 cgcgtactgg tcggcagtca gcaatcaatt ctcgccggat tagtggtggt cgccattgcg
     6601 ggtatgattg gttcgctact cggatgtcta tccggtgtgc ttggcggacg cgcagacgcc
     6661 atcatcatgc gcatcatgga cattatgctg tcgattcctt cgttggtact gacaatggca
     6721 ctggctgccg ctctcgggcc gagtttattt aacgccatgc tggcgattgc tattgtgcga
     6781 attccctttt atgtgcgcct ggcgcggggg caagcattag ttgtacgcca gtatacctat
     6841 gttcaggcgg cgaaaacctt tggcgcgtct cgttggcatc tgatcaactg gcatatttta
     6901 cgtaactccc taccgccgct gatcgtgcag gcatcgctgg atatcggtag cgcgatttta
     6961 atggccgcca cgttgggatt tattggcctg ggtgctcaac aaccgagtgc tgaatggggc
     7021 gcgatggtag cgaatggtcg caactatgta ctcgatcagt ggtggtattg cgcatttccg
     7081 ggggcagcga ttttgcttac cgccgtcggg tttaatctct ttggtgatgg tattcgcgat
     7141 ctgcttgacc cgaaagcagg aggaaagcag tcatgaccca acccgttctg gacattcaac
     7201 agctgcattt gagtttcccc ggttttaacg gtgatgttca cgcgctcaac aatgtgtcct
     7261 tgcagattaa ccgcggcgaa attgtcggtc tggtgggaga atccggctca ggtaaatcag
     7321 tcaccgcaat gctgattatg cgtctgctac cgacgggcag ttattgcgta catcgggggc
     7381 agatttcact gctgggagaa gatgttctta acgctcggga aaaacagctt cgtcagtggc
     7441 gcggcgcacg agtggcgatg atctttcagg aaccgatgac cgcccttaat ccgacacgtc
     7501 gaataggtct tcagatgatg gacgtaatcc gccatcatca accaataagt cgtcgggaag
     7561 ccagagctaa agcgattgcc ctgctggaag agatgcaaat cccggatgcc gtggaagtta
     7621 tgtcgcgcta tccgtttgag ctttcaggtg gtatgcgcca gcgggtaatg attgcgctgg
     7681 cattctcctg cgagccgcaa ttgattattg ccgacgaacc gacgacggcg ctggacgtca
     7741 cagtacagtt gcaggtactg cgtttgctta aacataaagc acgcgccagt ggaactgcgg
     7801 tactgttcat cagccatgat atggccgtgg tgtcgcaact gtgcgatagc gtttacgtga
     7861 tgtatgccgg aagcgtaatt gaaagcggcg ggacggcaga cgttatccat catccccggc
     7921 atccgtatac cattggtttg ctgcaatgcg caccagaaca tggagtacca cgccagccat
     7981 tacccgccat tccagggacg gtaccaaacc tcacccattt gcctgacggc tgcgcttttc
     8041 gcgatcgttg ctatgcggca ggtgcacagt gtgaaaacgt cccggcgctg acagcgtgtg
     8101 gtgacaacaa ccagcactgc gcctgttggt atccgcagca ggaggtcata agtgtctgac
     8161 acgttattaa cgttacgcga cgtccatatc aattttccgg cccgtaaaaa ctggcttggt
     8221 aaatctacgg aacatgttca tgccattaat ggtattgatt tacagatccg ccgtggtgaa
     8281 accttaggga tcgtcggcga gtcaggctgc ggcaaaagca ccctcgcaca gcttttaatg
     8341 ggcatgctgc aaccgagcca cgggcagtac atccgttcag gctcacaacg cattatgcag
     8401 atggtgtttc aggacccgct ctcttcgctt aatccgcgct taccggtgtg gcgcatcatc
     8461 acagaatcgc tctggatagc taagcgtagt agtgaacaac agcggcgagc gttggcagag
     8521 gagttggctg tgcaggtggg tattcgtccg gagtatctcg accgcctgcc tcatgcgttc
     8581 tccggcgggc agcggcaacg catcgccatt gccagagcac tctcttcgca gcctgacgtg
     8641 attgtgcttg atgagccaac ctcggcgctg gatatctccg tgcaggcgca gatcctcaat
     8701 ttactggtaa cgctacagga aaatcacggg ctgacttatg tgctgatttc acacaatgtc
     8761 tcggtgatac gtcatatgag cgatcgggtg gcggtgatgt atctcgggca gattgtagaa
     8821 ctgggcgacg cgcaacaggt gttgactgct tcggcacatc catacacccg attattgctg
     8881 gattccctcc ccgccattga taaaccgctg gaggaagaat gagcattacg taaaacggag
     8941 ctgccgggaa accgcacgtt gccgcaaggc tgttttttct acgaacgttg ccctctggca
     9001 acccacggat gtgaagtccg gcaatcatta gcaataaggg aggacggacg tgagctccgg
     9061 tgctggcggg cgctgtagag atgctaacaa cgcatcaggc attcacgcgc ctgacgcgtc
     9121 atccggcaat gctttacgat ttcaactggt aatccagcgt aatttccgct ttcagtacct
     9181 gagagaccgg gcatcctgct tttgctttct ggatgatgcc gtcaaaggta gaggcatcaa
     9241 tacccggcac cgcaacttca ctcttcagtg cgattttcgt aatcgcaaaa ccggcatcca
     9301 ctttatccag cgacacatcg gcggtggtat caattgatgt tggcgtgaat cccgcttccc
     9361 ccagcattaa tgaaagcgcc attgagaaac atgcggcatg cgctgcgcca atcagttctt
     9421 cagggttggt tcctttttcg ccttcaaaac gcgtgttaaa tccatacggc tgttggttca
     9481 gcacgccgct ctcggtggat actgttccct tcccgcgttt gatatcgcct tcccagtgtg
     9541 cctgaccttt cttatggatt gtcattgttg ctctcctgtg ggcttaaaaa ggaaattata
     9601 gactacgtga gaaacagcac gaggataagc aggatattcc gaataaaata cggcgaaaat
     9661 attacttctg cacttaaatt aaattcccac aatagataac cgggaatata tatctcaa
//
LOCUS       DADLXX010000139         9525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLXX010000139 DADLXX010000000
VERSION     DADLXX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.141"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(435..641)
                     /locus_tag="JNX27_003426"
     CDS             complement(435..641)
                     /locus_tag="JNX27_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7884508.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JNX27_003427"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JNX27_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7884509.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JNX27_003428"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JNX27_003428"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY7884510.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JNX27_003429"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JNX27_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /locus_tag="JNX27_003430"
     CDS             4795..5367
                     /locus_tag="JNX27_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY7884511.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8042
                     /gene="kdbD"
                     /locus_tag="JNX27_003431"
                     /pseudo
     CDS             5360..8042
                     /gene="kdbD"
                     /locus_tag="JNX27_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8039..8716
                     /gene="kdpE"
                     /locus_tag="JNX27_003432"
     CDS             8039..8716
                     /gene="kdpE"
                     /locus_tag="JNX27_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY7884512.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8991..9095
                     /locus_tag="JNX27_003433"
     CDS             8991..9095
                     /locus_tag="JNX27_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY7884513.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9406..>9525
                     /locus_tag="JNX27_003434"
     CDS             9406..>9525
                     /locus_tag="JNX27_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7884514.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2116 a         2396 c         2640 g         2373 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa acatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctccccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttaccac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcta ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttaatcgca ctgtttttta ttcaacaagg
     1621 tgcgctgcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatactt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgaccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttaccg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctccgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gcgaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gctgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctatt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg actagccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctggcg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaac cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcagaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggtggcgct attttatgga cgctggcctt cggtggttgc caccgtcatt
     6721 aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat
     6781 gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt
     6841 actgctggcg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac
     6901 ttatatgaaa tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc
     6961 agcgaacaat ttattgcctc tacgtttcat gcccgcagtc aggtgttgtt gcccgatgac
     7021 aacggtaaat tgcagccgtt aacacatccg caaggaatga cgccatggga cgatgccatc
     7081 gcgcagtgga gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt
     7141 gtaccgtacc agattttgcc gctaaaaagc ggcgagaaaa cctacggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ctggaaccgg
     7621 gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggccactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt agctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggc attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 caccgccgcg cccgatccgc tggtgaaatt ttccgatgtt accgtcgatt tagccgcccg
     8461 cgtgattcac cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctagc
     8521 ggtgctgctc aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg
     8581 ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca
     8641 aaaactggaa caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta
     8701 tcggtttatg ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat
     8761 taaacctgcg ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca
     8821 cgaaattaca acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg
     8881 atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
     8941 atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata
     9001 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
     9061 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
     9121 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
     9181 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
     9241 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
     9301 tgatttattt atcagcggtg gagctttgct ttttttccgg cgtgatcgat ttctcctttg
     9361 agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa
     9421 attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt
     9481 gacgaaagta attatattga cgttgccgcc attattttat cagtc
//
LOCUS       DADLXX010000140         9519 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLXX010000140 DADLXX010000000
VERSION     DADLXX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.142"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(194..1447)
                     /locus_tag="JNX27_003435"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="JNX27_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="JNX27_003436"
     CDS             1554..2447
                     /locus_tag="JNX27_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7884515.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="JNX27_003437"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="JNX27_003437"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7884516.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="JNX27_003438"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="JNX27_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7884517.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNX27_003439"
     CDS             complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNX27_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7884518.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYKVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVG
                     LLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTL
                     FIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEY
                     QLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNX27_003440"
     CDS             complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNX27_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7884519.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6671..7525)
                     /locus_tag="JNX27_003441"
     CDS             complement(6671..7525)
                     /locus_tag="JNX27_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7884520.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNX27_003442"
     CDS             complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNX27_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7884521.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8155..9240)
                     /locus_tag="JNX27_003443"
     CDS             complement(8155..9240)
                     /locus_tag="JNX27_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7884522.1"
                     /translation="MTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQ
                     DDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSA
                     KFERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKT
                     TGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIH
                     PIPAYTSGFDGWDAPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDA
                     QARGIRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSI
                     NILTSHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2473 a         2412 c         2460 g         2174 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 tcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagctc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actttgtaga gtaacgcgtc gctatgatta
     5221 atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc
     5281 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     5341 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     5401 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     5461 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     5521 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     5581 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     5641 ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga
     5701 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     5761 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     5821 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     5881 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     5941 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     6001 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     6061 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     6121 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     6181 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     6241 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     6301 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     6361 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     6421 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     6481 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     6541 cttccgcact ctctggagcg taataaaaca gcgctcccaa cacgcgcgcc gcgacagaaa
     6601 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     6661 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     6721 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     6781 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     6841 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     6901 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     6961 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     7021 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     7081 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     7141 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     7201 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     7261 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     7321 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     7381 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     7441 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     7501 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     7561 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     7621 tgcgcgcgcg gcagtggcgc gacggcagcc agagtaccgt gcttctggcg cagctcttca
     7681 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     7741 tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac
     7801 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     7861 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     7921 catgcggggt tatcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     7981 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     8041 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     8101 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     8161 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgctagcg gtgaagggcg
     8221 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     8281 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     8341 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     8401 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     8461 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
     8521 cagtggatag gttttccgca ggggagcgtc ccagccatca aaacctgagg tatacgcagg
     8581 aatgggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
     8641 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
     8701 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
     8761 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
     8821 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
     8881 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
     8941 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
     9001 gtcttcttgc tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc
     9061 cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
     9121 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
     9181 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
     9241 tttcgtacca tggtcgatgg catctgtcca tgtaaacatc tggggtcctt acagctcgtc
     9301 ctgacatgcg gtctcgcctt atatgcacat ggagaacatg gtcactccag accaacacta
     9361 atcaacatta accctattca ctttcgttaa gcactttttg gtgggtccgt ttggatgcat
     9421 tgaataggtg cggactccac atggagacag aaagtgcttt accaaaggag catcctttat
     9481 gaataccaac gaagttagcg tcatcaacct tgaccagct
//
LOCUS       DADLXX010000141         9041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLXX010000141 DADLXX010000000
VERSION     DADLXX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.143"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNX27_003444"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNX27_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY7884523.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNX27_003445"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNX27_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNX27_003446"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNX27_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7884524.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="JNX27_003447"
     CDS             complement(2403..2606)
                     /locus_tag="JNX27_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884525.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="JNX27_003448"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="JNX27_003448"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7884526.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITHLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="JNX27_003449"
     CDS             complement(3802..4014)
                     /locus_tag="JNX27_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY7884527.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="JNX27_003450"
     CDS             4274..4582
                     /locus_tag="JNX27_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7884528.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNX27_003451"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNX27_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7884529.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNX27_003452"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNX27_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7884530.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="JNX27_003453"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="JNX27_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY7884531.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="JNX27_003454"
     CDS             complement(8478..8981)
                     /locus_tag="JNX27_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7884532.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2314 c         2149 g         2338 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccgc acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccaattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacattt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat t
//
LOCUS       DADLXX010000142         9012 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLXX010000142 DADLXX010000000
VERSION     DADLXX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.144"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JNX27_003455"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JNX27_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7884533.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JNX27_003456"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JNX27_003456"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7884534.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JNX27_003457"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JNX27_003457"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7884535.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="JNX27_003458"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="JNX27_003458"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7884536.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="JNX27_003459"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="JNX27_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7884537.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="JNX27_003460"
     CDS             5545..6018
                     /locus_tag="JNX27_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7884538.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="JNX27_003461"
     CDS             6047..6589
                     /locus_tag="JNX27_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY7884539.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="JNX27_003462"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="JNX27_003462"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7884540.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="JNX27_003463"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="JNX27_003463"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7884541.1"
                     /translation="METYAVFGNPIAHSKSPLIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="JNX27_003464"
     CDS             7986..8243
                     /locus_tag="JNX27_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7884542.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="JNX27_003465"
     CDS             complement(8219..8773)
                     /locus_tag="JNX27_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7884543.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2256 a         2190 c         2248 g         2318 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaacg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gcgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaac gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gtcgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc taggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatgacaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taatatcatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc actcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggaaccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag ag
//
LOCUS       DADLXX010000143         8993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLXX010000143 DADLXX010000000
VERSION     DADLXX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.145"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNX27_003466"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNX27_003466"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7884544.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNX27_003467"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNX27_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7884545.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="JNX27_003468"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="JNX27_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7884546.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /locus_tag="JNX27_003469"
                     /pseudo
     CDS             complement(5771..7252)
                     /locus_tag="JNX27_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNX27_003470"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNX27_003470"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7884547.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="JNX27_003471"
                     /pseudo
     CDS             complement(7822..8133)
                     /locus_tag="JNX27_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNX27_003472"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNX27_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY7884548.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNX27_003473"
     CDS             complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNX27_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY7884549.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGATVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2253 a         2326 c         2133 g         2281 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccaga gtttggtacg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ctgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaacagag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg gggtcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaatcag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccctccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtcgct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctaccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctatgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaaa attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgtag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta tta
//
LOCUS       DADLXX010000144         8983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLXX010000144 DADLXX010000000
VERSION     DADLXX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.146"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..924
                     /locus_tag="JNX27_003474"
                     /pseudo
     CDS             1..924
                     /locus_tag="JNX27_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="JNX27_003475"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="JNX27_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7884550.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNX27_003476"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNX27_003476"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7884551.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="JNX27_003477"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="JNX27_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7884552.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="JNX27_003478"
     CDS             4447..4806
                     /locus_tag="JNX27_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7884553.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNX27_003479"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNX27_003479"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7884554.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="JNX27_003480"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="JNX27_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY7884555.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="JNX27_003481"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="JNX27_003481"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7884556.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2042 a         2175 c         2427 g         2339 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactgatat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagagttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtc
//
LOCUS       DADLXX010000145         8896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLXX010000145 DADLXX010000000
VERSION     DADLXX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.147"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            369..2887
                     /locus_tag="JNX27_003482"
                     /pseudo
     CDS             369..2887
                     /locus_tag="JNX27_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JNX27_003483"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JNX27_003483"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY7884557.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="JNX27_003484"
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="JNX27_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7884558.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5343..5564)
                     /locus_tag="JNX27_003485"
     CDS             complement(5343..5564)
                     /locus_tag="JNX27_003485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7884559.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(5760..7283)
                     /locus_tag="JNX27_003486"
     CDS             complement(5760..7283)
                     /locus_tag="JNX27_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY7884560.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKARVAALDSLRQQLKKCPVSAMHVYPAHLTDCLWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQK"
     gene            complement(7280..8042)
                     /locus_tag="JNX27_003487"
                     /pseudo
     CDS             complement(7280..8042)
                     /locus_tag="JNX27_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
     gene            complement(8039..8698)
                     /locus_tag="JNX27_003488"
     CDS             complement(8039..8698)
                     /locus_tag="JNX27_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7884561.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2168 a         2358 c         2324 g         2046 t
ORIGIN      
        1 tggttgtcct gctgatgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaacggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc gggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggatg
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag caattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt gtcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtggaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt ctcttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacaggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagcgccg ctaccctcgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcgccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtacagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aataaagtcg ctgacgaacg gtcagcacaa tatccgtggc gaggatatca
     7801 ttaagcccca attccccacc ctgcactttt tgcgacatat aagccatcgc ttcattgacg
     7861 gcgagcatcg cgccttcgcc cccctgcgaa acgctacctg cgccatcagc aaggaacatc
     7921 accagtaatg gctgctggtc atttagccag gcaacctgca tctggcaggc atcctggcaa
     7981 ggcaagtccg cgctgatgtg cgacgttcca acagcggatg cataaaccag acgccagctc
     8041 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcggg
     8101 aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt aatccttgca
     8161 gaggcaaagg ctgacgaacg ctgatttgtg ccagagtctt catatccgcc ccctgaacac
     8221 cgatggaaaa gaaagcaaac cttttatctt cttccccccg aaacactttg ttggctgcag
     8281 cctgccactc atcggttggt gcaccatcgg taatcaggaa aatccacggg cgataatacg
     8341 aaataccatt tgcacgatac tcacgtttcc gctcctctac catatccagg gctttggtaa
     8401 tggccgcacc cattggtgta tcgccctggg caaacaggat gggcggaaaa aaattagcgg
     8461 cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatgccg agttccactc
     8521 tttttaaagc cagcgaatcg gcaagcagct catcgcgaaa ggtaaccaat ccggtgttaa
     8581 gttcattaat aggtctgcca ctcatcgagc cagaaacatc cagcagcaga atgcaggggc
     8641 aacgtagttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
     8701 gaaatccttt cttagagaat tattgaggtc cggtatcata ccgactgcaa taatgaaata
     8761 agagcaaacg aagaaacttt cagaattagt cccggcggcg cagaaaattt tgctattcgc
     8821 ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa tttcaggagt
     8881 gagggttagg gagagg
//
LOCUS       DADLXX010000146         8889 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLXX010000146 DADLXX010000000
VERSION     DADLXX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.148"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..220
                     /locus_tag="JNX27_003489"
     CDS             <1..220
                     /locus_tag="JNX27_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY7884562.1"
                     /translation="ALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQ
                     LQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            247..1022
                     /locus_tag="JNX27_003490"
                     /pseudo
     CDS             247..1022
                     /locus_tag="JNX27_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(1086..1577)
                     /locus_tag="JNX27_003491"
     CDS             complement(1086..1577)
                     /locus_tag="JNX27_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7884563.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1679..2233)
                     /locus_tag="JNX27_003492"
     CDS             complement(1679..2233)
                     /locus_tag="JNX27_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7884564.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2257..2994)
                     /locus_tag="JNX27_003493"
     CDS             complement(2257..2994)
                     /locus_tag="JNX27_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7884565.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3049..3987)
                     /gene="ghrA"
                     /locus_tag="JNX27_003494"
                     /pseudo
     CDS             complement(3049..3987)
                     /gene="ghrA"
                     /locus_tag="JNX27_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4221..4308
                     /locus_tag="JNX27_003495"
     tRNA            4221..4308
                     /locus_tag="JNX27_003495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4255..4257,aa:Ser,seq:gga)
     gene            complement(4458..5300)
                     /locus_tag="JNX27_003496"
     CDS             complement(4458..5300)
                     /locus_tag="JNX27_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAY7884566.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELIDRLNDIIAKHYPD
                     MLAV"
     gene            complement(5385..5582)
                     /locus_tag="JNX27_003497"
     CDS             complement(5385..5582)
                     /locus_tag="JNX27_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY7884567.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(5594..6085)
                     /locus_tag="JNX27_003498"
     CDS             complement(5594..6085)
                     /locus_tag="JNX27_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884568.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(6082..6456)
                     /locus_tag="JNX27_003499"
     CDS             complement(6082..6456)
                     /locus_tag="JNX27_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY7884569.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(6503..6877)
                     /locus_tag="JNX27_003500"
     CDS             complement(6503..6877)
                     /locus_tag="JNX27_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY7884570.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(7040..7261)
                     /locus_tag="JNX27_003501"
     CDS             complement(7040..7261)
                     /locus_tag="JNX27_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY7884571.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(7324..7800)
                     /locus_tag="JNX27_003502"
     CDS             complement(7324..7800)
                     /locus_tag="JNX27_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY7884572.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7816..8301)
                     /locus_tag="JNX27_003503"
     CDS             complement(7816..8301)
                     /locus_tag="JNX27_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7884573.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(8392..>8889)
                     /locus_tag="JNX27_003504"
     CDS             complement(8392..>8889)
                     /locus_tag="JNX27_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY7884574.1"
                     /translation="TLQETRGGSGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEG
                     AYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRR
                     EDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETL
                     LESMR"
BASE COUNT         2194 a         2285 c         2255 g         2155 t
ORIGIN      
        1 agcgttagat aactttactc aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa
       61 tattaatacc ggtaaaccgg gccgcatggt gaccaacgat tatattgttg atattgctaa
      121 ccgcgatggt caattgcagt tgaacgtgac agatcgtaaa accggacaaa cctcgaccat
      181 ccaggtttcg ggtttacaaa ataactcaac cgatttttaa gccccagctt cataaggaaa
      241 ataatcatgc agcgcttatt tcttttggtt gccgtcatgt tactgagcgg atgcttaacc
      301 gccccgccta aagaagccgc cagaccgaca ttaatgcctc gtgctcagag ctacaaagat
      361 ttgacccatc tgccagcgcc gacgggtaag atctttgttt cggtatacaa cattcaggac
      421 gaaaccgggc aatttaaacc ctacccggca agtaacttct ccactgctgt tccgcaaagc
      481 gccagggctt acaaaacctg cttaacgagc gcaaggttat tcgtgcggca caagaaaacg
      541 gcacggttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca aatattatgg
      601 ttgaaggttc gattattggt tatgaaagca acgttaaatc gggcggtgtt ggggcaagat
      661 attttgggat cggtgcagac acgcaatacc agctcgatca gattgccgtg aacctgcgtg
      721 tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag acgatacttt
      781 cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg ctggaagggg
      841 aagtgggtta cacctcaaac gaacccgtta tgctgtgtct gatgtcggct atcgaaacag
      901 gggtcatttt cctgattaat gatggtatcg accgtggtct gtgggatttg caaaataaag
      961 cagaacggca gaatgacatt ctggtgaaat accgccatat gtcggttcca ccggaatcct
     1021 gacagataaa aatgccggaa ttaataatcc ggcatttact gtttaagcga aagcgtgagt
     1081 gcccctcacg ctttattttt gatttcctgc tcacggtgag ccgtctttgc actacgcgcc
     1141 gccagccaca ggccgctgtt tttcattgcg taaccaaata ccagcccaag cgccagcgaa
     1201 ggtaacacca gtttccagtc accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc
     1261 cccggaacaa atgaaagcag cagctgtttg gcctgaatac acatcagaaa cgcgacaata
     1321 ccggttatga cataaccgag aatttccaga tgtggtgcca gtgcactacc gtaaataatg
     1381 accatcgccc acaccacgcc actaagcagg gttgcagcgg agatcgccag ccctttcagc
     1441 ccaccttgcg ggcaagcaaa gtaggccgta cagcccagga agcccgccca gcttagtaag
     1501 cccagggaaa cagccaccca tccccagata ccggagagaa tgcctgttgt gattgcaata
     1561 gagagaagta tgttcatgcc gcgcatgata gcagaaaacg cggccatgaa tgagatcgag
     1621 cacacatttt atgcaacaac aaattatctg ttgcagttat gagtgacgat gatcacattt
     1681 actcttcaga atcttcttcc agctcgtccc acattgcact aatggcatca cgggttagcg
     1741 gtgccatggt gcgccagaaa ggcgtggttg catgggcctc cactttgccg aggaacgcac
     1801 cacaccaggg taacagatac tcactgaaca gtgtttccag tgcttcgctc tcatcttccg
     1861 ttgactgatc ttccagccag gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg
     1921 tatccgccag tggcatccct cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt
     1981 cctcaaccca tgcgctacga tatggcggca cggcacattc atcgccgata aacaacgcat
     2041 tgtaatcggc agagacttgc gtcatatcac agcttttctg taaacgcgtc agtaactcat
     2101 cctgctccag tggccagttc gcagccagtt tcccctcacg aatcagagta aacagcggca
     2161 ccagtaaagg atcttgcggt tggcggtaat agagcgaacc cagcacacga cagaggatag
     2221 aaaactcgtt cattaatata tttccattac tagtaattaa aggtctgcaa attccgcaat
     2281 cggtgccata ccgcgagatt caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat
     2341 gcgctctggc ggaaaatcta ccgcgtcgag gattttaaga cactcttcaa attcccccat
     2401 ggtaaacgcg gtgtgagaat ccgagcctaa tgccacccaa cctccagcat cacgtaccgc
     2461 tgcagctacc gcacgacagt tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt
     2521 attgatttcc agcgccacct gatgtttcgc ggctgcttca gcaacggctt taacatctat
     2581 ttcatatttc ggatttccgg gatggcttat tatatgcaca ttgccgctgg cgatagtggc
     2641 gatcatcgct tgtgtattgg tcgctttgtc atgtggcgca aaaaccggct catgaaaacc
     2701 ggcaataatc agatccagcg agtcaaacat tttaccgctg cagtcaattt caccatcaac
     2761 atttttaatg ttagcttcaa tgccgcgcag gatccctacc ccatcaacca ctcgcggcca
     2821 gatacgcatg ttaatgaagt gccagtgatg cggcgcatct tccatatccg ggccatgatc
     2881 ggtgatcgca aaaagtttaa tgcccttttg tttggcctgg gcaatgtaat cactcaatgt
     2941 gctatatgca tgtgtgctgg caacggtatg catatgaagg tcgacgggat acataactct
     3001 ctcctgtatt caattcccgc caaggatagc aggaatcctg atgctttatt agtagccgcg
     3061 tgcgcggtcg acttgcccgc agaccctctc ccctttttcg agctgggcaa tagtgcgaga
     3121 aatgtactcc acagcttcag cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac
     3181 gcgtggatgt tgccagagcg gactttcagg cggtaagggt tcacgattaa aaacatccag
     3241 cattgcgcct ttaactttcc cgctatccag cgccgcgagc aggtcatctt ccacaacatg
     3301 aacaccacgc gccaggttga ggagatacgc gccatcaggt aatttttcga gtaattgttg
     3361 attaataatg ccgacggttt caggggtatt cggtaacaaa ttaatcaata cccgacattg
     3421 gctcagaaat gcagacagtt cttcccgtcc ggcaaagctt tgcacgccag gccacgattt
     3481 acgggttcga ctccagcaac gcagcggaaa gcgctaggtt tgcagactct gagcaacttt
     3541 acagcccagt acgcctgcgc ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg
     3601 cagcggttgc caatgcgaac tattttgctg gatgcgataa tcatcaaaac gtcgaaacca
     3661 atgcagcacc tgactgacag catattcctg catttgctcg cccataccgg tatcttccag
     3721 gcgaaaaagt ggaacagaag ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat
     3781 agaatcaaca ccggccccga gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc
     3841 aacaggagga tgccagacta aaacataatc agcagagtca ttatctccgc ttttccatgc
     3901 cctgactctt gcctgaggaa tagctttgcg cagtgcctca atccaccatt gggtatcgaa
     3961 cgttgggtga taaaagatga tatccatact gactcccgaa aagcgttgtg cgaaatttat
     4021 tcgcacttat cgttatgatc tacaaaggcc accagcataa caaatccgtg gtcggtggca
     4081 aaaaaagcag atttcgctta ttaaaaccac acattgattg aagtttgaat aaacgcgcga
     4141 ttctttcaaa aagtttgttg acctcaggtc atgatttccc taaattagcg cccgttccag
     4201 caagacagga acgacaattt ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag
     4261 tgtgtatacg gcaacgtatc gggggttcga atccccccct caccgccaga ttataagcgc
     4321 atgttattgt ttaacatgcg cttttttatt ttcccttcca gacagtatgc catcgggtat
     4381 gccatctgaa accggtttat atgccccgcg atcatatctt atcggacaat ttccgatctt
     4441 tgagcatact ggcaaagcta tactgccagc atatcaggat agtgttttgc gataatatca
     4501 ttcagtctgt caatcagttc tggttttctg aacgtgatat gcgcggagcc cttcttaaag
     4561 tatctgatgc tgaacatctc atcttcatag ctctctttac cctgaacaga ctgaatgtga
     4621 tcatccagac gaagggtgat gtttccccgg ttgtcgggga ttggtttacc gctgaacagg
     4681 tgcaacattc tttccagatc ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat
     4741 ccccaccggt cccatctcac cagattgttg acgataattt tactgccaaa tttacagggg
     4801 caattggttt tgtaattcca gttcagccct ctgaagacgt tgatcacccc tcgctcaaac
     4861 acctcatcct tattctggtg cagttgttca aatgtgctca gaatgttcgc ttcgctgatt
     4921 tccggaaaat tatcgtactc cagtgaccgg taccatgtat cacgggcctg cgtatccatt
     4981 aatgacagca tcccggaacg ttgcatcagg tcacgccaga tttcgcgatc aagattccgg
     5041 gtaatggctt tcattgctgt ttcgggtttc tccatcagcc agcagccgca gcgaaaatcc
     5101 tgtttcatgg cccagtccgg ggcagtttta cctccgatgc tttccgtcag caccgagatt
     5161 tcagccagtt gtcggataag tgtgtcgatt tgcaacagtg tactctttcg tacagccaga
     5221 atgtggcgaa tgctggttga gcagataacg tccgtatgcc cgttgaccac ttccggacgg
     5281 gagattgtga tatctgacat aatcgatatt cctttttgca gataaatgaa aacgccagcc
     5341 ctgtgcaggc tggcgtcaga ggaatgaagt ctggtgagtg agcttcaccg gttctggtgc
     5401 agaagctgca gatgacgcag ggtacggaca tcctcccggg cctgttcgat acagggcaac
     5461 agtgctgctg aatctgtttt atcctcatcg ttgtcaaaga taattcccga tccgcagtcg
     5521 atattgtcct gcagccacgc aatcagaata tccagcgctg tttccgtggt taatgatttc
     5581 atgttgttcc tggttaccag tcgaaagtgg gtaaacccgg caggcattca gcgctggcat
     5641 ccagatgaat gagactgacg ccataacgcc ggatgagtgt gacgaccaga cgacggaacg
     5701 tttttgacag ccccaggcgt ttaagccgca gaaccgggta tgaccacgca tcggtacgta
     5761 acagatatcc gctacctgta aaatgaatcc attctgattc accgaagtca ctggtctggt
     5821 gtgacagcga gtacaaccag gcgttgtccg tttccgtgat atgtgcggta ctgcagcgta
     5881 tgccggtaag ggtagcaaat ggtggcgtgc attctgctga ggtctggtca gattcatcaa
     5941 caacacgaag ggagtagccg ttatcgcaga ccatgttaat cagttcagcg agctcaatac
     6001 catcgacgtc aacgacaatg cgccccatgt tcagtgcctg tacgttaacg ctgtcggctt
     6061 ccagtgtcag ggccagtttc atcatttcgc ctccggatac ttacccaagg taatgttatt
     6121 taccgttctg taattgtcgc gggtcatcag gccggtcgcc cggcgagccc ggaggatatc
     6181 aatactgttt attaactgag agcggggaca ggtgctgaat cccggctggt cggtacgcac
     6241 cagcgcgtat ttttccacga gaaaattaac tgcatcacac agtgaaatgc ctgcctcaat
     6301 atgctgctca atcacacgtt catcggcaaa cggtgtgtca ttcagtgtga ggccataatg
     6361 ctggtccagc agtcggatga gcagtgtctg ccagatggtg acgggagacg ggcagcgaga
     6421 tacctcccgt acatgtgtgt cggataatgt tttcatctta agttctctgt aactggtagt
     6481 gctaaaggtg agagcttcgg ggttatgagg tggttgcgag tgctggtgtc ggataaacag
     6541 ccagataaac gtaaccacag gagccgaggg tgtcggcttc gcaggtcagc ccttttgcat
     6601 tcagcgtgac ggtatgctga tatcggggat tcagttcacc gctggtgagc atgagttcca
     6661 gttgtttcat cagcagcgga aaggcctggt ccagatggta tgcatctgcg tcgctgaagc
     6721 ggcctctgat accggcgcgg tcggcaaggt aatgcaggcg gttaccctct tgcaccagac
     6781 gagcgccaaa acagggcctc actgtgcagg gaagccccca ccaggggcgg tcgtgattgt
     6841 catcgggatg agttatcccg gagagtttgt ctgacacgat aaaatcctct ctgaaaagtg
     6901 gttaagaatg gtttaacgat gacaggtcag ccggcgcgct gaccggtttc ggtgaagatg
     6961 tatgcattga tgagaatggc ctcatccaca gcggcgtctg aggtcatctg gtcgtactcg
     7021 tttcccgggt gacggaacat cagttcaggg tgacactcat cagccggaca tacgggccaa
     7081 aactgtcctt acggcgttct gcaaacacgg caagaacgcc gggaatatcc tgcacttcac
     7141 gaccagtata cgcttcagca ctgccgtgcc agcgatactt accggtacag aacggaaaaa
     7201 gacgggatgt cggatgttgt tggtgaatac gcatggcttc accacgggtg ataattttca
     7261 taatgggata cctctgaaga cagaagataa aagtgaaaac aggtgtgatg tggttgtgaa
     7321 gggttaaagc agaccatgtt cggcaaagga gaaaacctgg ttgccaccga ctatcagatg
     7381 gtccggcacc cggatatcca ccaggcccag tgcctgtacc agacgttcgg tgataagccg
     7441 gtctgcctta ctgggtgtga cttcaccgga cgggtgattg tgcgccagca ccacggcagc
     7501 ggcattgtgg tacagggcgc gtttaatcac ttcccgggga tgaacttccg tacggttgat
     7561 ggtgccggtg aagagggttt caccggcaat cagctgattc tggttgttca gatacagcac
     7621 ccggaactct tcccgctcca gtcccgccat gttcagaatc agccattccc gtgccgcacg
     7681 ggtggaggtg aaggccacgc cgggttcatg aagatggcgg tccagggttt gcagggcccg
     7741 cagaatgaga ctgcgctcgc cgggcgtcat ctctccgggc agaaaggaaa gctgctgcat
     7801 tgtccctgcc tccattcagt caatgatgcg cataatggcg ctgcattccg gatgctgcag
     7861 ggcgtaatcc cgcagccggt aatagtggac cgtcatggca taacactccg tacgacaggc
     7921 atgatgactg tacgtcatca gacaggcagc aataccggcg gcttccgggc tcatttcagc
     7981 gcggttgccg ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt catccggttc
     8041 gggtgccata aatgccccgc cgttgttcag ggtgtacaga ttccagatac caccgcagta
     8101 gtcttcgcaa agacggtcca tccagccgaa gacacggggc tccagggtca cccactgtgg
     8161 aatgaggcca aaatgctgcg gccagaagct gatgcgctgt tcatcaggga cgggagtggc
     8221 aaccagctga ggtgggttat tccctgatgc agtggttacg gaaacagaag gtgtggtgga
     8281 attatgcgaa acggttgtca tgaggttatt ccttataaaa agtaaatgaa tggaagaagc
     8341 cccggggcag gaacagatgt gagtcaggac tgcactttca gtgaaacggc atcagcgcat
     8401 gctctccagc agcgtttctg ccatcaccca caatgcgcga ttgagcttaa tgtcggtatc
     8461 aatgctgtga atggcccggg tatggatacg ttttcctttt gcactgcgac cggaaatccc
     8521 gcctttcagc atattctcct ggatggtctg ataagcactc cacaggtcct taccgtaatc
     8581 ctcccggcgt cgtggtgtca gaatgtcggc ggtggtgacg ggctgatgtt cgtcaccata
     8641 acggtaagtc agtgccgcct gtgccagcgc ctggcgtgcc ggtggcggca ggaccagcga
     8701 ctgcatggca tcacgcttct cctctatccg gtcaaaaacg cccaccacct cgtaagcccc
     8761 ttcaatgact ctgtccacca catttccccg gtgtggaaca cgcaattctc ccagagactg
     8821 accgcagacc agtccattgg tacaaatggc acgaaaatat cctgaaccgc cccgggtttc
     8881 ctggagagt
//
LOCUS       DADLXX010000147         8861 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLXX010000147 DADLXX010000000
VERSION     DADLXX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.149"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1179)
                     /locus_tag="JNX27_003505"
                     /pseudo
     CDS             complement(1..1179)
                     /locus_tag="JNX27_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1389..1832)
                     /locus_tag="JNX27_003506"
     CDS             complement(1389..1832)
                     /locus_tag="JNX27_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884575.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2347..3189
                     /locus_tag="JNX27_003507"
     CDS             2347..3189
                     /locus_tag="JNX27_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884576.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3296..3559
                     /locus_tag="JNX27_003508"
                     /pseudo
     CDS             3296..3559
                     /locus_tag="JNX27_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4186..4731
                     /gene="cobU"
                     /locus_tag="JNX27_003509"
     CDS             4186..4731
                     /gene="cobU"
                     /locus_tag="JNX27_003509"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY7884577.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4728..5471
                     /gene="cobS"
                     /locus_tag="JNX27_003510"
     CDS             4728..5471
                     /gene="cobS"
                     /locus_tag="JNX27_003510"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY7884578.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5483..6562
                     /gene="cobT"
                     /locus_tag="JNX27_003511"
     CDS             5483..6562
                     /gene="cobT"
                     /locus_tag="JNX27_003511"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7884579.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6624..7559
                     /gene="ldtA"
                     /locus_tag="JNX27_003512"
     CDS             6624..7559
                     /gene="ldtA"
                     /locus_tag="JNX27_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7884580.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7615..7690)
                     /locus_tag="JNX27_003513"
     tRNA            complement(7615..7690)
                     /locus_tag="JNX27_003513"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7655..7657),aa:Asn,seq:gtt)
     gene            8017..>8861
                     /gene="nac"
                     /locus_tag="JNX27_003514"
     CDS             8017..>8861
                     /gene="nac"
                     /locus_tag="JNX27_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY7884581.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQA"
BASE COUNT         2257 a         1972 c         2257 g         2375 t
ORIGIN      
        1 ccacaggtct gtcgcccctt cagttgccag ttcgctgtct ttacagacgt aatctttttt
       61 catgcgaccg aaggcttccc tgacaaaatc atcgacctag gcttcatcaa caggaagggt
      121 tccgtttttc cgtaactcat gaagcgcacg gttagccagg tatgcctggc gtgaggatcc
      181 gggataaacg cgggaggcgg gcagcacatc gctgttcagc attgccggta ccttctgaca
      241 gactgcatca gccccatcgc cattacggtc tttttcatca aatttattga ccagcacaaa
      301 caggcggccg actgacacat cagcaatggc attaagctct ttacgaacct cctcatcggc
      361 tttcgagttc atctgggtgt agtccatcac cgccagcacc gcagaggctt tctgcagttg
      421 atcccgcatc atcacttcca tctgcggttg tccggcttca ttagggccgg gggtatccac
      481 cagtgtgagt gttccctggc attcatccat ccccaccaga tggctgaatt ccacgtcgat
      541 caccggcagt ttctgcactt ctgcgtattc atcaaaagga aattcactcc ccattgccgc
      601 agcaagacga accagatcgt ttaacgatgc cagtccggta aagattccct cctccccgtg
      661 gtattcattt ttgagccagc catcatccgt cagaatatcc agcagttcgc gggtatcccc
      721 ggtttgctgc agggtctgtg cgacctgctg tcctgccggg gtggcgactt tttcctgcag
      781 tgtgattaat aaattgcgga caggctgaat atgttccaga tggagaaccg gctcagtttt
      841 tccgggaacg tggcggatga gcgtcggtac agaggtcatg gggcggttac ggttaggcag
      901 aatttcctgc cccacaatgg cgttgatggt ggttgatttg cctgctttca tcgtgccgac
      961 aacagccagc accatctccc tgcgggcggt tttgatttgt tcgccctcca gctccttaat
     1021 tcttttcagt gctttctgtt tatcaaaaag ctgcccgttt tcgttcttac cgtcagataa
     1081 cacatccggc tcatcaacca tttgccggag cagattgatg ctcagttgca aaagccggtc
     1141 ggcttcatca caaagcaggg tgatgttctt ttcgtgcatt atttaacctt cctgagacgc
     1201 attttcatga caaccacgga ggaggccatt tactacaagc gatcgtttat aacaatcaat
     1261 ataatagaaa cgatcgtcat attctattaa tatgaaaaac acaacgcaag ggggacaacc
     1321 cgggtatggc tgcgtttgca ttgtgttttc atagagttaa aaatctgtaa cgacaacccg
     1381 gcacatcact accgggttaa gaagaaaatc cagttccata ccgcgcgggc cacagagacc
     1441 acggtgtcac gaacggcgcg cagggccgta cggacaacag aggcaacaca gacactctcc
     1501 gccgtgtcaa atatccggtc caccgcaccg gtaaactgtt cacgggtctg agtgcggaca
     1561 gactccgtgc gcagttcgtc ctgcaggcgt gtcatcagag ggatggcggc atgtccggga
     1621 agcactgtca tgagtgcact gaccggggtg acctccccct gaaacagtgc attacagagt
     1681 gaggatttac cggccccgct tttacccata atgccaggca cgggttcgtg acgggggagt
     1741 ttgcgcagat gttccaggat gtgacgggaa atcgggtaag gcagggagga gagcggtttt
     1801 tcaattgcct caatggcatc agacggattc atacatgtat tcccagtgaa aaaagacaaa
     1861 accccgtgca ccggaaaggt ggcgggggat tcacggagat aatatctttc tgaaattatt
     1921 ttgaatgcag gtatatgtgg ctgatttgta tcagctaatg agtacaggtc tgaatctaat
     1981 ctgacagtcc gctttgtgcc aggagcggac tgacctgctc cccgatgatt aatacacagc
     2041 tatgctagta atgtctgtag atcgctgtgt gttgcatcta tcggttgaac tcacagtcca
     2101 aaccagactg tcagctttga ttagattcta cctactcaac ctgtcagata aggtctgagc
     2161 taatacatct aatatatggc tttgtgcagg ggacggctat cgcgaacgtt aggctaggta
     2221 tctgaatacg cacagttcac ttggttgtac ttcttgcact gatctgctat cccgcacagc
     2281 tatttatagg taatcttcag tgtataatct gccgacttat ggaagcttgt acaacaggag
     2341 aaatatatga aaaaagaatt gtttggacgt tgcccgtttg atctcgacga agatgtaaaa
     2401 aaaatagccg cgataaataa atatatccat gcaaaatttg aggagcataa aaataaaata
     2461 gacaacttaa agggtgtgga acaaaaattg atggtcttac agtactttaa tgtactgaat
     2521 gaacttagta atcaggctat ctcttctttg aatataggag ccttttcagc atcggaagta
     2581 ttagctaggg ttataataga acaggctgcc aatcaatttt atatagcaat agatgatggc
     2641 gaaaatgcac aagcattact caaggctagt aagaaattag ttcatagtaa tggaaaacgg
     2701 tggctggagt gtttaaaatc caaaggaatg actaatccag cagcaaatga aaggattcgc
     2761 atgggaaaag aactaactga tctatttaat agattgtggc caaatacacc tgaatatcca
     2821 ggaaccagaa aactttttga attaattggc tgggaaacac actatcatgc ccattacgtt
     2881 cccctttgtg attcaataca tacattctct gatgatatgg taaatatagt atctctatat
     2941 aatgcaatcc aaagtgatga aactactgct atcgaattca ttcttgcagt taaacaagaa
     3001 aataaacggc tggcaattta taattttgta attgctacgg cccttcggtg tgaggcctta
     3061 gtaaatgtat ttaactcttt aggttatcag gatattataa cagaaatgac accaacaata
     3121 gatacagtca accagactat aattcgatat gatgattttg atcattccag aattgatgat
     3181 tgtcaataat taattgacag aatacgtaca tgcgcaacaa ctagtgatag ttgacttatt
     3241 ttcgctcttg agagacaacc atactcaaat ctcccacatt gcaggagatt tgaatatgaa
     3301 aacgtcaccg tggaataaag accgtaccat cggtcaaaaa aggccacttc agatatctca
     3361 tatctggggg atccggatta gacttgagtt ggaaggtaaa acacgcgatc tagctctgtt
     3421 caacatggct ttggacagta agctacgggg ctgtgatttg gtcaaactca aagtatcaga
     3481 tgctgcatat ggcagttctg tctcaagcag agcaacggtg ttgcaacaga aaaccggcag
     3541 ccctgtccaa tttgagatgc ctttaataac acatctttta aatgttgaag aattatcccg
     3601 cctgctgaag aacgttgctc tgagtgtgat aaaccttttt attcttgatg atgtcagcga
     3661 gaagatggaa tatatccccg actgggtcgt ggttacccgg ttaaacatct ggtggcgcta
     3721 ataaatctgg caagtcacat tctgttgaat gctgtgacag tgccttaccg agagagcgaa
     3781 attgtggtgt tctagtgcat agtcgccacc attccggata attctattgc gttgttttac
     3841 aagttctttt acagcgcaga tctgtcgctg acgttgaatc aatagcctgg atttcagaac
     3901 acaatgcccg tttgctggtt ggtccggcag tagcattaat tcattatctg aatgaaatgt
     3961 gtcaatccac agaaaccgct gtcaatgacg cattttgtcg gacgttaaca cgagaaagtc
     4021 tggctattcc gtaatgccat cttatttcgt aatggattga gaatgctgga tttttaatta
     4081 tattacgcgc agtattaatc gcgtaaatgt tttgcggtca gcaacggtgg gggatccatt
     4141 gctctgttca atgaatacca tcatacattt gaatcaggag tcggtatgat gattttggtg
     4201 acgggaggcg catggagcgg gaagagtcgc catgcagagg tgcttattgg ggactcttca
     4261 caggttctgt atatcgctac ctcgcaaatc cttgatgatg agatggctgc acggatagaa
     4321 catcatcggc aaagccgccc ggagcactgg cgcacagtgg agcgctggca acatcttgat
     4381 gaattaattc atgcagacat taacccgaat gaggctgtgt tgctggaatg cgttaccaca
     4441 atggtgacta atctgttgtt tgattatggc ggcgataaag accctgatga atgggattat
     4501 caggcgatgg aacaggcgat taatgctgag attcagtcgt tgattgctgc ctgccaacgt
     4561 tgccccgcaa aggttgtatt agtgactaac gaagtgggaa tggggattgt gccggagagt
     4621 cgtctggcac gacattttcg tgatattgcc gggcgggtaa atcagcagtt ggccgctgcg
     4681 gcaaatgaag tatggctggt ggtttcgggt attggagtaa aaatcaaatg agcaaattat
     4741 tttgggcgat gctctcattt attacgcgtc tgcctgtacc gcgtcgctgg tcccagggac
     4801 tggatttcga gcattattct cgcggtatta ttacttttcc tttgattgga ttattgcttg
     4861 gcgcgattag cgggctggtc ttcatggtgc tgcaggcatg gtgtggcgca ccactggcgg
     4921 cactgtttag cgtacttgtg ctggcgctga tgaccgggga attccatctg gacggcctcg
     4981 ctgatacctg tgatggcgta ttttctgcac gtagctgcga tcgcatgttg gaaatcatgc
     5041 gtgatagccg tttaggcacc cacggcggtc tggcattgat ttttgtggta ctggcaaaga
     5101 ttctggtgtt aagcgagttg gctctgcgtg gcgaaccgat ccttgcatcg ctggcggcag
     5161 catgtgcggt cagccgcggg actgccgcat tattgatgta tcgtcatcgt tatgcgcggg
     5221 aagaagggct tggcaatgtg ttcattggca aaattgatgg gcgacaaacc tgcgtcacac
     5281 tcggcttagc cgctattttt gctgccgtat tattgctggg tatgcatggt gtggctgcta
     5341 tggtggtgac gatggtggcg attttcatcc tcggtcagct tctcaaacgt acgctgggcg
     5401 ggcaaacggg cgatacgctg ggagcagcca tagagcttgg tgaactggtc ttcctgctgg
     5461 cactgctata aagagacatg acatgcaaat aattgccgat ttactgaata cgatcccagc
     5521 catcaattct gccgctatgt cgcgtgcaca acggcatgtt gacgggttac tcaaacctgt
     5581 tggtagccta ggaaagctgg aggcgcttgc catacaactg gcaggaatgc cgggggtgaa
     5641 tggcataccg catgtgggca aaaaagcggt actggttatg tgtgccgatc acggcgtctg
     5701 ggaggaaggg gtcgctattt ctccaaaaga agtgacagcc atacaggctg aaaatatgac
     5761 ccgtggaaca accggcgtgt gtgtgctggc agcacaagcg ggcgctaacg tccacgtaat
     5821 tgatgttggt attgatactg ctgagcctat ccccgggctt atcaacatgc gtgtcgcacg
     5881 aggtagcggc aatattgctt cagctccggc aatgagtcgc cgtcaggctg aaaagttgct
     5941 tttggacgtc atatgttata cgcgggagct ggcgaaaaac ggtgtcacgc tgtttggtgt
     6001 aggtgaactg gggatggcaa acacgacacc ggcagcggca atagtcagca caatcactgg
     6061 ccgggatcct gaagaagtgg ttgggattgg cgcaaacctg ccgacagata aactggctaa
     6121 taaaattgat gttgtgcgtc gggcgattac gttgaatcaa ccaaatcctc aggatggtgt
     6181 tgatgtcctg gcaaaagtgg gtggatttga tttggtcgga atagctggag tgatgttagg
     6241 tgctgcttcc tgcggtttac ccgtgttgct ggatggattt ctttcttatg ctgctgcgct
     6301 cgcagcctgc cagatgtctc ctgcaatcaa accgtatctc attccttctc acttgtcggc
     6361 agaaaaaggc gcgcgtatag cgctctcgca tttggggctg gagccttatc tcaatatgga
     6421 tatgcgttta ggtgagggga gtggtgcagc tctggcgatg tccatcatcg aagctgcttg
     6481 tgcgatatac aacaacatgg gcgaacttgc tgccagtaat attgttctac cggggaatac
     6541 gacttctgat ttgaacagct aaaaacgaaa agtctgataa aagctatact tatcggatag
     6601 tattcgcgac ttaggaaagg gacatgatgc gtcgtgtaaa tattctttgc tcatttgctc
     6661 tgctttttgc cagccaaaat agcctggcgg taacttatcc attacctcca gagggtagcc
     6721 gtttagtggg gcagtcgctt actgtaactg ttcctgatca caatactcag ccgctggaga
     6781 cttttgcctc acaatacggg caagggttaa gtaatatgct ggaagcgaat ccgggcgctg
     6841 atgttttttt accgaagcct ggctcacaac tcaccattcc gcagcaactg attttgcccg
     6901 ccactgttcg taaagggatt gtggttaacg tcgctgaaat gcgtctttat tactatccgc
     6961 cagacagtaa tacagtggaa gtctttccta ttggtatcgg ccaggctggg cgagaaaccc
     7021 cgcgtaactg ggtgactacc gttgaacgta aacaagaagc accaacctgg acgccaacgc
     7081 cgaacacccg gcgcgaatat gcgaaacgag gggagagttt gcccgcattt gttcctgcgg
     7141 ggcccgataa ccccatgggg ctgtacgcga tttatattgg caggttgtat gccatccacg
     7201 gtaccaatgc caattttggt attgggctcc gtgtaagtca gggctgtatt cgtctgcgta
     7261 atgacgatat caaatatctg tttgataatg ttcctgtagg cactcgtgta caaattattg
     7321 atcagccagt gaaatacaca acggaaccgg atggttcaaa gtggctggaa gttcatgagc
     7381 cgctgtcgcg caatcgtgca gaatatgagt ctgaccgaaa agtgccattg ccggtaaccc
     7441 catctttgcg ggcgtttatc aacgggcaag aagttgatgt aaatcgcgca aatgctgcgt
     7501 tgcaacatcg atcgggaatg cctgtgcaaa ttagttctgg ttcaagacag atgttttaag
     7561 agcgttggta attagaaagt aaaaagcctg ctcgaaagca ggcttttttg aatttggctc
     7621 ctctgactgg actcgaacca gtgacatacg gattaacagt ccgccgttct accgactgaa
     7681 ctacagagga atcgtgtgaa cggggcgcat attaatggca gtgcatggtg atgtcaaagg
     7741 gtgaaatgca tttttttgtt tgtttgctgc caatttcaac aaagtggtga gctttcaatc
     7801 ttattgggta atgaaccatc gtggtgcata ccctcctttt atagggcagg tgaatgcgac
     7861 tgctgataaa aagggccaat gacgaatcta tcataatctc ttatttgaca gttggttagc
     7921 ttgcgcatca acgccaaaat agaactggca ggcatcttgc atactggttg taagtaatgg
     7981 cggcacttgg gccgattctt aaaaaccgga ggcaacatga acttcagacg cctgaaatac
     8041 ttcgtaaaaa ttgtagatat tggtagcctg acccaggctg ctgaagtatt gcatatcgca
     8101 caaccagcgc tcagccagca ggttgccata ctggaaggtg agttaaatca acagcttttg
     8161 attcgcacaa aacggggcgt tacgccaaca gacgccggaa aaattctcta tacccatgca
     8221 cgggccattt tacgtcagtg tgagcaggcc caactggcgg tgcataacgt tggtcagtca
     8281 ttatcggggc aagtctcgat tggctttgcg ccaggaaccg ctgcgtcatc catcaccatg
     8341 cccttattac aggcggttcg cgctgaattc ccggaggtcg ttatctatct tcatgaaaat
     8401 agtggcgcag tgcttaacga aaaactgata aatcaccaac tcgatatggc ggtgatttat
     8461 gagcattccc ctgtggctgg ggtatccagt caggctttgc tgaaagaaga tcttttcctg
     8521 gtaggaactc aagattgccc ggggcaaaac gttgatgtta atgctattgc gcaaatgaac
     8581 ctttttctcc ccagtgatta cagcgctgtt agacttcgtg ttgatgaggc tttttcccta
     8641 cggcggctca cggcaaaagt cattggtgaa attgagtcta ttgccacgct taccgcagcg
     8701 attgccagcg gcatgggcgt tgcagtatta cctgaatcgg ctgcgcgttc gttatgtggc
     8761 gcagtaaatg ggtggatgtc acgcattacc actccttcaa tgagtctctc tttgtcctta
     8821 aatttacccg ccagagcaaa cttgtcacca caggcacagg c
//
LOCUS       DADLXX010000148         8771 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLXX010000148 DADLXX010000000
VERSION     DADLXX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.151"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNX27_003515"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNX27_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7884582.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="JNX27_003516"
     CDS             complement(984..1814)
                     /locus_tag="JNX27_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY7884583.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="JNX27_003517"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="JNX27_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7884584.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="JNX27_003518"
     CDS             3240..3590
                     /locus_tag="JNX27_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY7884585.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="JNX27_003519"
     CDS             3716..4276
                     /locus_tag="JNX27_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7884586.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7245
                     /locus_tag="JNX27_003520"
     CDS             4279..7245
                     /locus_tag="JNX27_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY7884587.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7312..7689
                     /locus_tag="JNX27_003521"
     CDS             7312..7689
                     /locus_tag="JNX27_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7884588.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7890..8549)
                     /locus_tag="JNX27_003522"
     CDS             complement(7890..8549)
                     /locus_tag="JNX27_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7884589.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2480 c         2348 g         1993 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg
     5581 accggcttca tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg
     5641 cgctactacg aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc
     5701 tgggtgcagg gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag
     5761 ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa
     5821 tatctgcatg agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg
     5881 gcagccaacg acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg
     5941 gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg
     6001 cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac
     6061 ttcacgcact tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc
     6121 ctggccgacg cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc
     6181 tacgcgaagc tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg
     6241 ttggctgaac tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc
     6301 accacatcat catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg
     6361 cacatcaacc caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac
     6421 caatacgcgc cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg
     6481 ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg
     6541 gcgggcttca ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg
     6601 cgcatccgcg acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac
     6661 gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac
     6721 gaaatcctgc ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc
     6781 aggaaactcg gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc
     6841 cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc
     6901 gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc
     6961 aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc
     7021 ctcaacctgg tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg
     7081 catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca
     7141 ctcggctggg agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc
     7201 ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc
     7261 gttttctgag acgaccccta ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat
     7321 tatctctgta aaaagttatt caaccggctt tccggtaccc tggtgatcag agcgcgctgc
     7381 ggcaataaca ttactggcct ggcatgttgc aatattcttt atccctcgcc ccgatacagc
     7441 ggtcagctgc atattaaaga gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt
     7501 aaagcgataa tgcgctttat agcccggctt gcgcttgaac aggaatgcct tagccttagc
     7561 tggaacgctg aaaaatccaa ccccggcgct aaccgttttt atcaggctct gggaggcaga
     7621 ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
     7681 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7741 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7801 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     7861 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7921 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     7981 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     8041 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     8101 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     8161 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     8221 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     8281 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     8341 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     8401 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     8461 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8521 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8581 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8641 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8701 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8761 caaagtgcgt c
//
LOCUS       DADLXX010000149         8592 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLXX010000149 DADLXX010000000
VERSION     DADLXX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.152"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..323)
                     /locus_tag="JNX27_003523"
     CDS             complement(<1..323)
                     /locus_tag="JNX27_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7884590.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(372..800)
                     /locus_tag="JNX27_003524"
                     /pseudo
     CDS             complement(372..800)
                     /locus_tag="JNX27_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(800..1507)
                     /locus_tag="JNX27_003525"
     CDS             complement(800..1507)
                     /locus_tag="JNX27_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7884591.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="JNX27_003526"
     CDS             complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="JNX27_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY7884592.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2204..2914)
                     /locus_tag="JNX27_003527"
     CDS             complement(2204..2914)
                     /locus_tag="JNX27_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7884593.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="JNX27_003528"
     CDS             complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="JNX27_003528"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7884594.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4333..5553
                     /gene="astC"
                     /locus_tag="JNX27_003529"
                     /pseudo
     CDS             4333..5553
                     /gene="astC"
                     /locus_tag="JNX27_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5550..6383
                     /gene="astA"
                     /locus_tag="JNX27_003530"
                     /pseudo
     CDS             5550..6383
                     /gene="astA"
                     /locus_tag="JNX27_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6379..7308
                     /locus_tag="JNX27_003531"
                     /pseudo
     CDS             6379..7308
                     /locus_tag="JNX27_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            7305..>8592
                     /gene="astB"
                     /locus_tag="JNX27_003532"
     CDS             7305..>8592
                     /gene="astB"
                     /locus_tag="JNX27_003532"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY7884595.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2002 a         2208 c         2358 g         2024 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgtcaaaatg cgcagccagt cgggtacgca gggcatcatc gactgttggc
      421 ggtgtgacgc ttgcggcttc ggtgtactcc agcgccagcc gttcttcatc gctaaagagc
      481 gggctttgcc gccagtcagc cactgccagc agtttatcgg tgctgccggt acgctcggcg
      541 actttcatac tggtgatatc cacgcaaaac tcacacaggc acatttgcgc aatgcgcgcg
      601 ctgacaagcg atcgcactac cggatcgagc ggtgagcgtt tgcgctccag ccacccaaca
      661 aacattgaca ccaaataaaa gataaacggg atccgccccc accagcgaat cgggcttagc
      721 acctcgccgt aatggcgacg atgcagccac gcctgcggac gtaaaatgaa aggagttttg
      781 ctaagcggcg gtaaacccat cagtcattcc tcttctggcg ctcaagccat attttcttcg
      841 ccataaaaat caccacggtc agggcaaaca gaataaacag ttcggtgaca aaccagaaag
      901 taccgcctgt taacatgctg cccgcccagg aataaacaat agtcgccggt aattgaccaa
      961 gcccggtggc gataaaaaac gagcgaaaac gtattgaagt cagaccggca gcatagctga
     1021 ttggatcgaa agggacaaaa ggcaataacc gacagaccag gatggtgtgt ttgccgtagc
     1081 gagtgaaaaa gccgtccata ctgtcaagca cggttttgcc ggttaatttt tccaccactt
     1141 cgcggcccat cactctggcg ataaaaaagc acagcgccgc gccggccatc gaactggtcc
     1201 acgacagcaa gccgccccag aacgcgccaa acagcgacgc attggcaaag gtgatcaaaa
     1261 acgcaggtag cggtgcagca atggcctgta aaatcatcaa caagaacgag acaactgccg
     1321 ccagtgcgcc gtaagactga ataaagcgtt ctataccctg ttggtccacg gcggcaaacg
     1381 ccgcaacgct gcgattgata aactcatgga cgccgggaag cagcgcccat gcgagcagca
     1441 tagcgaacaa caggatgatg agggttatac ggtagtacca gattttacgc gattgtatct
     1501 tcatcatcct ttatcctggc tttattcggt gattttaaag gtaaccgttg ccagcgtgtt
     1561 atccgccggg agaactgagg cattgccttt gaatttcact tcgccgcgtt tttccaccgc
     1621 accataagtg tatgttgcat tgctgacgat gccgaccggg cagctatcca gacacaccag
     1681 gcaacccgtt ttcttctctt ctgctgccgt taaattaccg ccaaagcgca tgtccagttt
     1741 cttgccatta ctgtcaacaa tcacttcatc gaaggaatac gcttttgccg ccccttgcca
     1801 gttgaccgaa atatccagtt tgctgcctgt gacatgagtc gtttctttat tatccatcgt
     1861 catgttttcg cccggcgtac cacctgcctc tttcagggct tcataaaatg ctttcggggt
     1921 cgcataagcc ataaacagcg atttatgtcc gttggagcca tctttaaaca caataccgtg
     1981 acgagtgtcg tcggtgagga aacgaccatt aatttgcacc agcatggtta ccgtatggtt
     2041 attggcatcg acgcgcagcg gttggctgtt cgaaagtccg tcgtattcca cttttgccgc
     2101 cgcattctct ttttgatcgc agccgctaag cccggcaaca gccagtaagc agagtgcagc
     2161 cagtcctttt ttccatgaca ctgaataatg ttgcgacatc gttctatcct tcattttttg
     2221 ggtgagtaag tgggctgcgg cgcgaagcag acagatccac atgtttgtgt cgggcgtaga
     2281 gttttgcgag ctggatgaga ataaacagcg ccaggcccgc cagacagagt tgtaaaataa
     2341 agcgcagcgt aatgccttca ttggcgagat cgcttgccat cacggtataa ataacaatac
     2401 cgggcagggt cgtgagtgcc gaaataaggg tataaggcca gaaggcgatg gtggttaatc
     2461 cgtaagcgta attttgaata ttgtaaggaa acaacgggat taagcgggtc agaataagaa
     2521 aatcaatacc gttacgcgca atgccttttt caatggcctg gaaggtatgg ctatggccca
     2581 cgtatttcag cagtaaatca cgtcccagcc agcgcgccag caggaatgag cacgaggagg
     2641 ccagcgttgc ggcaattaat gagagtagtg tccctaagag cgggccaaaa actattcctc
     2701 cagcgatcac cagtatgctt cctggtaaca gaaagagggg ggcattgatg aataacagaa
     2761 tatagaggtt ataaccgaac aatccgctct ggcgaatgag tgtctgtaag tggggtaaat
     2821 cggtgagcag atcgaataaa ccgaaagcgt ggatagcgta aatgaccagc gcaaaaataa
     2881 gacaggcaaa cagaaagtta cgctcagcgt tcatcgtgga gtttcctata tcaaacttga
     2941 taatagagaa gtggtcagac aactaaaaac gcagtgacgg tataacaaag gacggcaggc
     3001 aacaaatcat tactgacagt ttgttgattt ttctcaaggt taattctcct gacccagttt
     3061 gagccaggag agctgctaaa ttagcggcgg aaggtcgccc agacgggggc gtgatcggac
     3121 ggtttttcca tgctgcgaat ttcatagtcg atgccggttt ctacgcaaca ttctgccagc
     3181 ggttggctgg cgagcagcag gtcgatgcgc agaccacggt tatcgtcaaa accttttgag
     3241 cggtaatcaa accatgagaa acgatctgct gtttgcggat tagcatggcg gaaggtatcg
     3301 accacccccc agctcatcag cctgtccatc cattcgcgct cttccggcag gaaagagcat
     3361 ttaccggtac gcagccagcg cttacggttt tcttcgccaa tgccgatatc cagatctgta
     3421 gggctgatat tcatatcgcc cataatcagt accggattat cacgtttgag ttcggtttcc
     3481 aggtagtttt gcagattctg ataaaactgc gcttttgccg ggaattttat cggatggtcg
     3541 cggctttcac cctgcgggaa gtaaccgttg atcacggtga cataacccag cagtgagggg
     3601 atttccgcca taataatccg ccgctgcgcc tcttcgtcgt caccgggaaa gccgcgacgc
     3661 acggcaatcg gcgtctcttt ggtcagcagc gccacgccat aatggccttt ctgcccgtga
     3721 taaaacacgt tgtagccgag cttcgccacc tcttcgagcg gaaacatatc gtcatgaact
     3781 tttgtctcct gcaggccaat cacatccggt tggtgctttt cgacgatggc ttcaagctgg
     3841 tgaggtctgg cgcgcaggcc gttgatatta aaagagacaa atttcatagt cgctgccatt
     3901 tattcagata aagagtgcgt ggatggtagc agaatttcgc gttgcttacc gcctgttgtt
     3961 aagtgatttg atcgatggtt gttaatgatg tcaacgatgg cgcaaaaaat gcccgctttt
     4021 ggtgcgcgct gcgtcagaat ggcgcagtaa tttccagtaa atttcgtcaa tgatcacaat
     4081 tccagaatta tattttactt ctgcataaca ttgcgttttt tattcttttt attcacccac
     4141 atcgcaaacg tattcacttt atatgcactt taaatgcata tggtttggtt ataactcctt
     4201 gatttccagt tagcctccgc cgtttatgca cttttatcac tggctgacac gaaccctgca
     4261 atctacattt acagcgcaaa cattacttat tgttaacata caaataacga attatttact
     4321 gtagaggtcg ctatgtctca gccaattacg cgtgaaaact ttgatgaatg gatgatacct
     4381 gtttacgctc cggcaccctt tataccggta cgtggcgaag gttcgcgctt gtgggatcag
     4441 caggggaaag agtatatcga cttcgcgggt agcattgcgg tgaacgcgct gggccatgcg
     4501 catccggaac tgcgtgaagc gctgaacgaa caggcgagta agttctggca taccggcaac
     4561 ggttacacca acgagccggt actgcgactg gcgaaaaaat tgatcgacgc cacgtttgcc
     4621 gatcgcgtct tcttttgtaa ctccggtgcg gaagccaacg aagcggcgct aaaactggcg
     4681 cgtaaattcg ctcacgaccg ctacggcagc cataagagtg gcatcgtggc gttcaaaaat
     4741 gcgtttcatg gtcgcacgct gtttactgtc agtgcgggtg ggcagccagc ctattcacag
     4801 gattttgcgc cactgccggc ggatattcgt catgctgcat ataacgatat taactctgcc
     4861 agcgcgctga ttgacgacgc cacctgtgcg gtgattgtcg agcccatcta gggcgaaggc
     4921 ggtgtggtgc cagccagcaa cgcgttttta caaggtctgc gtgaattgtg tgaccgccac
     4981 aatgccttgc tgatttttga tgaggtacaa accggcgtcg ggcgcactgg tgagctgtat
     5041 gcctatatgc actacggcgt gacgcccgat ctgttaacca ccgccaaagc gctgggcggc
     5101 ggtttcccgg tcggtgcgtt gttggcaacc gaagagtgcg cccgcgtgat gaccgttggc
     5161 actcatggca ccacctatgg cggtaatccg ctggcctcgg cggtagcagg caaagtgctg
     5221 gagctcatca acacaccaga gatgcttaat ggcgttaaac agcgtcacga ctggtttgtt
     5281 gagcgtctta atactattaa tcaccgctat ggtttgttca gtgaagttcg cggcttaggt
     5341 ttgttgattg gctgtgtact gaatgccgat tacgccgggc aagcgaaaca gatctctctg
     5401 gaagcggcga aagcaggcgt gatggtactg attgcgggtg gcaacgtggt gcgttttgcg
     5461 cctgcgctca atgtcagcga agaagaggtg acgaccggac tggatcgctt tgcagctgct
     5521 tgcgaacact ttgttagccg aggttcatca tgatggtcat ccgtcccgtt gagcgctcag
     5581 atgtctcggc gctgatgcag cttgccagca aaacgggcgg cggcctgacg tcgcttcccg
     5641 ccaatgaagc cacgctttcg gcgcgtatcg aaagggcaat caaaacctgg caaggcgaac
     5701 tgcctaaaag cgagcagggc tatgtgttcg tgctggaaga tagcgagaca ggcaccgtgg
     5761 cggggatttg cgctattgag gtggcggttg ggctgaacga tccctggtac aactatcgcg
     5821 tcggcacgtt ggttcacgcc tcaaaagagc tgaatgtcta taacgcattg ccgacgctgt
     5881 ttcttagtaa cgatcacacc ggcagcagcg agctgtgcac gctgtttctc gacccggact
     5941 ggcgcaaaga gggcaacggc tatttgctgt cgaaatcgcg ctttatgttt atggcggctt
     6001 ttcgcgacaa gtttaatgac aaagtggttg ctgaaatgcg cggggtgatt gacgaacacg
     6061 gctattcacc gttctggcaa agcctcggta aacgcttctt ttcgatggat tttagccgcg
     6121 ccgattttct ctgcggcacc gggcaaaagg catttattgc agaactgatg ccgaaacatc
     6181 cgatctatac ccacttttta tcccaggaag cccaggacgt catcggtcag gtacatccgc
     6241 aaaccgcgcc tgcccgcgcg gtgctggaga aagaaggttt tcgttaccgc aactatatcg
     6301 acatcttcga cggcgggcca acgcttgagt gtgacatcga ccgcgtgcgc gccatccgta
     6361 aaagtcggct ggtggaagtg gcagcaggct ggcttgccgc cgggcgtgct gaacctggtg
     6421 cagggcgggc gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg
     6481 tttaccggta gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag
     6541 aaaattctcg cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat
     6601 atcgacgcgg ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc
     6661 acctgcgccc gccgtttatt gctcaaaagc ggagcgcagg gcgatgcgtt tcttgctcgt
     6721 ctggttgccg tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt
     6781 attggcgggc tgatttctga acaggccgca cagcaggtgg ttactgcctg gcagcaactg
     6841 gaagcgatgg gcggacgaac cctgcttgcg ccgcgcttat tacaagcagg gacatcgttg
     6901 ctgacaccag ggatcattga aatgacgggc gttggcggtg tgccggatga agaggtgttc
     6961 gggccgcttt tgcgcgtctg gcgttatgat acttttgatg aggcgattcg gatggcgaac
     7021 aacactcgct tcggactctt ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa
     7081 ctgttgctgg aggcgcgggc ggggattgtt aactggaaca aaccgcttac tggtgctgcc
     7141 agtaccgcgc cattcggcgg cattggtgct tccggtaacc atcgccccag cgcctggtat
     7201 gccgcagatt actgcgcatg gcctatggcg agcctggagt cggactcgtt aacattgccc
     7261 gccacgctta accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag
     7321 ttaatttcga cgggctggta gggctgacgc atcattacgc gggcctgtcg tttggtaatg
     7381 aagcctctac ccgtcaccgt tttcaggtgt ctaacccgcg actggcggca aaacagggct
     7441 tactgaaaat gaaagccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg
     7501 agcgtccgtt tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac
     7561 tggaaaaagt tgcacgccag gcaccgcact ggctttccag cgtcagttcc gcttcgccaa
     7621 tgtgggtagc caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc
     7681 atctcaccgt tgccaacctg aacaataaat ttcaccgttc gctggaagcg cccgtcactg
     7741 aatcgctgtt aaaagcgatt tttaaggacg aagagaaatt tagcgtccat tcggcgttgc
     7801 cgcaggtagc gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt
     7861 acggtgaacc gggtatgcta ctttttgtct acgggcgaga agaaggcaat gatacccggc
     7921 cttcccgtta tccggcgcga cagactcgcg aagccagcga ggcggtggca aggcttaatc
     7981 aggtgaatcc ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagagcg
     8041 tttttcataa tgacgtgatt gccgtgagta accgccaggt gctgttttgc caccaacagg
     8101 cgttcgctcg ccagtcacag ttactggcaa acctgcgggc gcgggtgaac ggttttatgg
     8161 cgatagaagt tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta
     8221 acagccaact gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc
     8281 gggaacacgc cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta
     8341 gcgaactaaa agtctttgac ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc
     8401 tgcggttgcg ggtggtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga
     8461 tgaacgatac cttgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgtc
     8521 ttactgctgc cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg
     8581 tattgagcca at
//
LOCUS       DADLXX010000150         8332 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLXX010000150 DADLXX010000000
VERSION     DADLXX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.153"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..894)
                     /locus_tag="JNX27_003533"
     CDS             complement(<1..894)
                     /locus_tag="JNX27_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7884596.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="JNX27_003534"
     CDS             954..1232
                     /locus_tag="JNX27_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884597.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="JNX27_003535"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="JNX27_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7884598.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="JNX27_003536"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="JNX27_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7884599.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="JNX27_003537"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="JNX27_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7884600.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="JNX27_003538"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="JNX27_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7884601.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="JNX27_003539"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="JNX27_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="JNX27_003540"
     CDS             complement(4912..5442)
                     /locus_tag="JNX27_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7884602.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="JNX27_003541"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="JNX27_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7884603.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="JNX27_003542"
     CDS             complement(6411..7634)
                     /locus_tag="JNX27_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884604.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2425 a         1615 c         1736 g         2556 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgctc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg cagcttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaactagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta ttgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcatttatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttggta gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg cacccaagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaataatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat gctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaagcca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcggt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaaggt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta
     8161 cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa
     8221 tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc
     8281 agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca gg
//
LOCUS       DADLXX010000151         8321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLXX010000151 DADLXX010000000
VERSION     DADLXX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.154"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            335..3184
                     /locus_tag="JNX27_003543"
     CDS             335..3184
                     /locus_tag="JNX27_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7884605.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDIEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3284..5212)
                     /gene="dhaR"
                     /locus_tag="JNX27_003544"
     CDS             complement(3284..5212)
                     /gene="dhaR"
                     /locus_tag="JNX27_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY7884606.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATMTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5431..6501
                     /gene="dhaK"
                     /locus_tag="JNX27_003545"
     CDS             5431..6501
                     /gene="dhaK"
                     /locus_tag="JNX27_003545"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY7884607.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6512..7144
                     /gene="dhaL"
                     /locus_tag="JNX27_003546"
     CDS             6512..7144
                     /gene="dhaL"
                     /locus_tag="JNX27_003546"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY7884608.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7155..>8321
                     /gene="dhaM"
                     /locus_tag="JNX27_003547"
     CDS             7155..>8321
                     /gene="dhaM"
                     /locus_tag="JNX27_003547"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7884609.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2069 c         2075 g         2003 t
ORIGIN      
        1 tcccataact aattaggatt attacaaaaa atcttcacca aattttttaa ccgctgtttg
       61 ttgtaatatt tttgccatat tcatagtaac gaattggaat aagtaacgac aacttaggga
      121 ataactcgat ataaactcac ctttgtaagg ggggtgttaa atatatatta attcctgtgt
      181 tcctgatttt gcccggtaat agagaattat tctcagagaa gctcatcatg attgcaccat
      241 acagatatca tgatgataag aggatattat tcattgactt aaacaaaatg acaaaatcca
      301 tagacccata gcctgggtac tatataggag tggtatgggc atcaaacaac acaatgggaa
      361 taccaaagcc gatcgtctcg ctgaattaaa aatccgttcg ccctcaattc aactgataaa
      421 atttggcgct attggtttga atgcaattat cttttccccc ctgctgatag ctgctgatac
      481 aggaagtcaa tatggcacca atattactat taatgatggt gacagaatta caggagatac
      541 cgccgatcca tcaggaaacc tctatggtgt aatgacccca gcaggaaaca cgcctggcaa
      601 tatcaacctg ggtaatgatg tcaccgtcaa tgtcaacgac gcctctggat atgcaaaagg
      661 aatcattatt cagggcaaaa acagctccct gacagctaac cgactcacag tagatgttgt
      721 tggtcaaacc tctgccatcg gcattaactt aattggtgac tatacccatg ctgacttagg
      781 cacaggcagc accattaaga gtaacgatga cggcatcatt attgggcata gctcaacact
      841 aacagccact caattcacca ttgaaaactc gaacggtata ggcctaacca tcaatgacta
      901 tggcaccagt gtcgatcttg gaagcggaag taaaatcaag accgatggaa gtacaggtgt
      961 ttatatcggt ggtctcaacg gcaataacgc caatggtgct gcgcgtttta cggcgacaga
     1021 cctgacaatc gatgttcagg gctacagcgc catggggata aacgtacaga aaaactctgt
     1081 tgtcgatctc ggaacaaaca gtaccattaa aaccaatggc gataatgctc acggcctctg
     1141 gagctttggc caggttagcg cgaatgcact cactgttgat gtaactggag ccgcggccaa
     1201 tggcgtcgaa gttcgtggtg gtacaaccac tatcggtgca gatagccata tttcttccgc
     1261 gcagggcggt ggcctcgtca ccagtggttc agacgcgaca atcaattttt ctggcacggc
     1321 agcgcaacga aacagcatct tttccggcgg ttcttatggt gcctcggccc agacggcaac
     1381 ggctgttatc aacatgcaaa ataccgatat tacggttgat cgtaatggca gtctggcgct
     1441 gggtttgtgg gcgctcagcg gcggtagaat aaccggagac agtttggcta tcaccggcgc
     1501 ggcaggagcc agagggattt atgccatgac caacagccag atcgacctca cgagcgatct
     1561 ggtcattgat atgagtacac ccgaccagat ggccatcgca acgcaacatg acgatggtta
     1621 tgccaccagc cgcatcaacg cctcgggtcg tatgcttatc aacggtagcg ttctttccaa
     1681 aggtgggcta atcaatctgg atatgcaccc tgggtcggtt tggacaggtt cctccctcag
     1741 cgataatgtc aatggcggga aactggacgt tgcaatgaat aacagcgtct ggaacgtaac
     1801 aagtaattct aatctcgaca cgctggcgct gagccattca actgtcgatt ttgccagcca
     1861 cgggtcaact gccggcacat ttaccacatt aaacgtagag aacctgagcg gtaacagtac
     1921 ctttattatg cgtgctgatg ttgttggcga gggtaatggc gttaataata gaggggattt
     1981 attgaatatc agcgggagta gtgctggtaa tcacgtattg gctatccgca accagggcag
     2041 cgaggccaca acgggaaatg aagttctgac agtggtaaaa accactgacg gcgcggcctc
     2101 gttcagcgcg tcttctcagg ttgagttggg gggatatctg tacgatgtgc gtaaaaatgg
     2161 cactaactgg gagctttacg cttccgggac agttccggaa ccgactccta atcctgaacc
     2221 cacaccagct cccgctcagc ctcccatagt caaccccgat cctacgccta aacccaccac
     2281 gaccgcagat gctggcggca attatctcaa tgtcggttac ttattgaact atgttgaaaa
     2341 ccgtacgctg atgcaacgga tgggtgactt gcgaaatcag agtaaagacg gtaatatctg
     2401 gttgcgcagt tatgggggaa gcctggactc ctttgccagt ggcaaactga gcggctttga
     2461 catgggttac agcggtatcc agtttggtgg ggataaacgt ctctctgatg taatgccgtt
     2521 gtatgtcggt ctgtatattg actcaacaca tgcatcgccg gactatagcg gaggcgacgg
     2581 taccgcacgt tcagactaca tgggaatgta cgccagttac atggcacaaa acggttttta
     2641 cagcgatctc gttataaaag catcgcgcca gaaaaatagt ttccacgtac tggacagtca
     2701 gaacaactgc gttaacgcca acggcactgc gaatggaatg agcatctccc tggaagccgg
     2761 gcagaggttc aacctgtccc ctactggtta tgggttctat atagagccgc aaacccagct
     2821 tacatacagc caccagaatg agatggctat gaaggcgagt aatggcctca atatacatct
     2881 gaatcactac gaatcgctgc tggggcgtgc cagcatgata ctggggtatg acatcaccgc
     2941 aggcaacagc cagctgaatg tctatgtgaa gactggcgct atccgcgagt tttcagggga
     3001 tatcgaatat ctgttgaacg actcccggga gaagtacagt ttcaaaggta atggctggaa
     3061 taacggcgtg ggagtcagtg cacagtataa caaacagcac acattctatc tcgaagcgga
     3121 ttacacgcag ggtaacctct ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt
     3181 ttaagtgcgg gatagacaag cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat
     3241 gaaatacaga tcactctgat gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa
     3301 actgccctgc atcaatgcca tgttgcttca ttttccgcca cagcgtagtg cggccgatcc
     3361 caagtaaagc cgacatttcc tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg
     3421 cctctttttc aacttccgca aatgacagac tggtggaaag gcgggtggcg ctgacatcat
     3481 ctgtcgcctg ctcggtaaac agatgttcag gtaaatcact gacgcgaatg cgcccgttat
     3541 cactactcag agcaagattc tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg
     3601 cacaagaaac taggcgagcg agggcatcgt catcaatttt cagccgcgta gagaagcgtt
     3661 tttcaagact gcgtaatttg ttattcacca gcgccggaat gctgccacgc cgcatacgca
     3721 gaggcgggat ggtaatttca aatgcatgca gcgcgtaata cagctggcga ctaaaacgat
     3781 tttgttccac cagcattgcg aggtccgcgg tcattgttgc aatcactttg acatcaattg
     3841 gtattaaacg ccgcgcatcc agtcgcgtga taaccccctg cttgataacc tgcagcaaag
     3901 cagactgtaa ctccaccgcc agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg
     3961 ccagttccag ccgactcaga cggccatttt cattgtccgt gcgatcgcca ccaataaatt
     4021 cttccgccag cgcagcatca ccatataact cacaattgac ggcgatataa ggacctgcag
     4081 cacgctcgct ttcattatga attgcctgac ttagcagtgc cttgcccacg ccctcttctc
     4141 cacaaagcag gacaggaaag ctactgcgcg ccgcctggcg accaaaatga atcaagcggc
     4201 gggtttgcgg atcgtcctgt ggcatatgag cgaaggtatg gctgactttt cctaattgac
     4261 tggtcattaa ctgccgcatc tgttccacag gatggagcaa caaaataaag ctggttccct
     4321 gcgtttctat tatcggttta agggttatca ccgcatcaat aaactggtgc tggctttcaa
     4381 aggttgcttc tacgtgtttg agcggatgtg cctgttttat tgcttgttgc aatacggcgg
     4441 gtaacgttaa gagttcagtg attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga
     4501 cccgcgccgc ctgggcatta ataaattgca aattaccctg ctcgtcccag ctaatcacgc
     4561 catcatccat actttctaac agggcattaa gttgatttaa atgacggtta gtttcagcga
     4621 gcaaactgtc cgtcagcagt aaattaccga cctcgcgggc gattgccaac gtcaacggca
     4681 aatcagctgc ggtagtttgc tcaaccggac acgccagcgc tattgttccc gtcaatcggc
     4741 ccttgctgtc aaacaacggc gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat
     4801 gttgatcggc catcgttttc acggcctgac cagagatagc cgctaacgat agcgcacaag
     4861 taccaataat tccctcggcg caatacgtgc cgtcattgaa tcccagtgca cttagctgct
     4921 gcaaggtttg cggatcgcca ttacggctga gaatgcaggc ggtttcatcg aggataaaca
     4981 gcgcacactc tcgcggtgcc atatattccc aggcatcttc cagcgcagcc tgcccgagcg
     5041 tcagcatcgc tttcttacgc cgataaatag aagcaaatgt cacgccctgg gcctgatgtg
     5101 gtacgttcca tgtctcccgt ttcatcagct tattgcatcg ctcccaggag gttgcaatta
     5161 agggagatat gccccgacca tcgttgttaa aagcgccact catatcccgc atcccttatg
     5221 acaaaaggtg ttccggctcg caattttaag tattaataca cgtaaaagtt tcaatatgga
     5281 acattcatta aagcatatgt ttcactatga aacataattt gggctaattt tcttttccct
     5341 tgcctgatgc acaatggatt catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc
     5401 atgccctacc gtaattgctg gcgcaaaata atgaaaaaat tgatcaatga tgtgcaagac
     5461 gtactggacg aacaactggc aggactggcg aaagcgcatc catcgctgac actgcatcag
     5521 gatccggtgt atgtcacccg agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg
     5581 ggtggcggca gcggacacga gccgatgcac tgtggttata tcggtcaggg gatgctttcg
     5641 ggggcctgtc cgggcgaaat tttcacctca ccgacgcccg ataaaatctt tgaatgctcc
     5701 atgcaaattg atggcggcga aggtgtactg ttgattatca aaaattacac cggcgatatt
     5761 cttaactttg aaacagcgac cgagttactg cacgatagcg gcgtaaaagt gaccactgtg
     5821 gtcattgatg acgacgttgc agtaaaagac agtctttata ccgccgggcg acgcggcgtt
     5881 gccaacaccg tattaattga aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg
     5941 gacgcctgtg cggaactggg gcgtaagttg aataatcaag gccactcaat aggtatcgct
     6001 ctcggtgcct gtaccgttcc tgccgcgggc aaaccttctt ttacccttgc ggataatgag
     6061 atggagtttg gcgtcggcat tcatggtgag ccaggtattg accgccgccc tttctcttcc
     6121 cttgatcaaa ccgtcgatga aatgttcgac accctgctgg aacatggctc ataccatcgc
     6181 actttgcgtt tctgggatta tcaacaaggc agctggcagg aagaaccaca aaccaaacaa
     6241 ccgctccagt ctggcgatcg ggtgattgca ctggttaaca atcttggcgc aactccgctt
     6301 tctgagctgt acggcgtcta taaccgcctg accacacgtt gccagcaagc gggattgact
     6361 atcgaacgta atttaattgg cgcgtactgc acctcactgg atatgaccgg tttctcaatc
     6421 accttactga aagttgatga cgaaacgctg gctctctggg acgccccggt ccacaccccg
     6481 gcccttaact ggggtagata aggagaaagc aatgtcactg agcagaactc aaattgttaa
     6541 ctggctcact cgttgtggcg atattttcaa caccgagagc gagtatctta ccgggctgga
     6601 tcgcgagatt ggcgacgctg accacgggct aaatatgaac cgaggcttta gcaaagtggt
     6661 ggaaaaactc cctgctatcg cagataaaga tatcggtttc attctcaaaa ataccggtat
     6721 gacgctgctt tccagcgtcg gtggtgccag tggtccgctg ttcggtacct tctttatccg
     6781 cgccgcacag gcgacccagg cacggcaaag cctgacactg gaagagcttt atcagatgtt
     6841 ccgcgatggc gcggacggcg taatcagtcg cgggaaagcc gaacctggcg ataaaaccat
     6901 gtgtgatgtg tgggtgccgg tggtggaatc gttacgtcag tccagcgagc aaaatctctc
     6961 tgttccggtg gcgctcgaag ctgccagtag catcgccgaa tccgcggcac aaagtacgat
     7021 tacgatgcaa gcccgcaaag gccgcgccag ttatctcggt gaacgcagta ttggtcacca
     7081 ggatcccggc gcgacctcgg tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga
     7141 gtaaggaatt ggtgatggta aacctggtca tagtttcaca tagcagccga ctgggagaag
     7201 gtgtcggtga attagcccgt cagatgttaa tgagtgatag ttgtaaaatc gccattgccg
     7261 cgggaattga cgatccacaa aatcccattg gtaccgatgc cgtcaaagtg atggaggcca
     7321 tcgaatctgt tgctgatgcc gaccatgtgc tggtcatgat ggatatgggt agcgcattat
     7381 tgagtgctga aactgcgctg gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt
     7441 gtgctgcgcc gttggtcgaa ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg
     7501 atatcgacaa agttatcttt gacgctatgc atgcgctgga agccaaacgt gaacaactgg
     7561 gtttaccgtc ctccgacact gaaatctctg acacatgtcc tccgtacgat gaagaagccc
     7621 gttctctggc ggtggtcata aaaaaccgta acggcctgca tgtacgtccg gcctcccggc
     7681 tggtttatac cttatcgaca tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg
     7741 tcacaccaga gagtattaac cagattgcgt tactacaagt tcgctataac gatacgctgc
     7801 gcctgattgc gaaagggcca gaagctgaag aggcactgat cgctttccgt cagctggctg
     7861 aagataactt tggtgaaacg gaggaagtcg ctccacctac tctgcgtccc gttctgcctg
     7921 tttcgggtaa agccttttat tatcaaccag ttttatgtac ggtacaggca aaatcaaccc
     7981 tgaccgcgga agaagaacaa gatcgattac gccaggctat tgacttcacg ttattagatc
     8041 tgatgacgtt aacagcgaaa gcagaagcca gcgggcttga cgatattgcc gcaatctttt
     8101 ctggtcacca tacactgtta ggtgatccgg aactgctggc ggcggcaagc gaactccttc
     8161 agcatgaaca ttgcacggca gaatatgcct ggcagcaagt tcttaaagaa cttagccagc
     8221 aataccagca actggatgat gaatatctac aagctcgcta tattgatgtg gacgatcttc
     8281 tgcatcgcac cctggtccac ctgacccaaa cgaaagaaga a
//
LOCUS       DADLXX010000152         8201 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLXX010000152 DADLXX010000000
VERSION     DADLXX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.155"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            59..460
                     /locus_tag="JNX27_003548"
     CDS             59..460
                     /locus_tag="JNX27_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7884610.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="JNX27_003549"
     CDS             570..1901
                     /locus_tag="JNX27_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7884611.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYTNL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /locus_tag="JNX27_003550"
                     /pseudo
     CDS             2225..3613
                     /locus_tag="JNX27_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
     gene            3695..7328
                     /locus_tag="JNX27_003551"
                     /pseudo
     CDS             3695..7328
                     /locus_tag="JNX27_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            7325..>8201
                     /gene="narH"
                     /locus_tag="JNX27_003552"
     CDS             7325..>8201
                     /gene="narH"
                     /locus_tag="JNX27_003552"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7884612.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKYIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2190 a         2031 c         1912 g         2068 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacagaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatac aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 acttggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatatatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc t
//
LOCUS       DADLXX010000153         8194 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLXX010000153 DADLXX010000000
VERSION     DADLXX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.156"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNX27_003553"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNX27_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY7884613.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISVLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNX27_003554"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNX27_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY7884614.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNX27_003555"
     CDS             complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNX27_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAY7884615.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPDFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNX27_003556"
     CDS             complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNX27_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY7884616.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNX27_003557"
     CDS             complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNX27_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY7884617.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3681..4508
                     /gene="nadE"
                     /locus_tag="JNX27_003558"
     CDS             3681..4508
                     /gene="nadE"
                     /locus_tag="JNX27_003558"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7884618.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4738..5625
                     /gene="cho"
                     /locus_tag="JNX27_003559"
     CDS             4738..5625
                     /gene="cho"
                     /locus_tag="JNX27_003559"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7884619.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNX27_003560"
     CDS             complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNX27_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7884620.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNX27_003561"
     CDS             complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNX27_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY7884621.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNX27_003562"
     CDS             complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNX27_003562"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7884622.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2153 a         2055 c         1890 g         2096 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtac gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcaatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagt
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc ccaaagctta
     2281 gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa
     2341 taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga
     2401 ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc
     2461 aatttaaata ttgcggtttt gatttatgaa ataactcttt gacgggaaaa tttaaaataa
     2521 attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat
     2581 cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct
     2641 ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac
     2701 ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt
     2761 ctgcctgtgc gcgcattttt gataccagta aagagataga catgcccgca gaacaaaaca
     2821 gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca
     2881 ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt
     2941 tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata
     3001 aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg
     3061 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
     3121 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
     3181 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
     3241 gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg
     3301 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     3361 ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata agctgtacaa
     3421 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     3481 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     3541 agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     3601 tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct
     3661 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     3721 aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc
     3781 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     3841 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa actggcaacg
     3901 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggctgac gaacaagatt
     3961 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     4021 cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     4081 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     4141 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     4201 atggtgacgg cggtacggat attaatccgc tgtatcgtct caacaaacgc cagggtaaac
     4261 agttactgac ggcattaggt tgcccggaac acctttataa gaaagcccca actgccgatc
     4321 ttgaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     4381 tcgacgacta tctggaaggg aaaaacctac ctgaacaggt cgccagaaca atagagaact
     4441 ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa cgttttcgat gatttctgga
     4501 aaaagtaata atttgcacct attggattgt gcgaaaaaga gtaatttgtt cacgccggat
     4561 gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatacat tgcaggagtc
     4621 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatca acccgcctct
     4681 tttttaatcg cctcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     4741 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     4801 gaacatttac attcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     4861 ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg
     4921 ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc
     4981 tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     5041 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     5101 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     5161 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag catcgctgat
     5221 gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagtcgcgg tcgtgcatgt
     5281 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     5341 catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa
     5401 ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     5461 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     5521 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     5581 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     5641 gtttaccttg cggttgtaaa agccacaggg tgtgcctgcc atcgaaccag cacgcgccct
     5701 ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     5761 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     5821 catccaggcg cgtcataata ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt
     5881 ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc
     5941 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     6001 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     6061 aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc
     6121 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     6181 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     6241 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     6301 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     6361 gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc
     6421 tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc
     6481 tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc
     6541 gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt
     6601 tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc gtggatctgc
     6661 tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac
     6721 ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg
     6781 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     6841 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     6901 cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     6961 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga
     7021 gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga actataccgg
     7081 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     7141 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     7201 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     7261 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca
     7321 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt
     7381 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     7441 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     7501 gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc aaaatgtctg acgctgaaat
     7561 gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     7621 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     7681 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccaacgcaca gattctttgc
     7741 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     7801 tttcagcaaa tagctgccaa cgaccgccaa acattcggtt catatcgcta tggcaataac
     7861 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     7921 ggatctcgct gtgagatatc gcgccaagca acgcgtccag catttccaca ggtgccgtct
     7981 cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca
     8041 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     8101 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     8161 cgctggaacg gataaaccga accgagattc agta
//
LOCUS       DADLXX010000154         8191 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLXX010000154 DADLXX010000000
VERSION     DADLXX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.157"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1242)
                     /locus_tag="JNX27_003563"
     CDS             complement(<1..1242)
                     /locus_tag="JNX27_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY7884623.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JNX27_003564"
     CDS             complement(1239..2117)
                     /locus_tag="JNX27_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7884624.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="JNX27_003565"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="JNX27_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="JNX27_003566"
     CDS             complement(3022..3255)
                     /locus_tag="JNX27_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884625.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="JNX27_003567"
     CDS             complement(3252..4109)
                     /locus_tag="JNX27_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY7884626.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="JNX27_003568"
     CDS             4134..4463
                     /locus_tag="JNX27_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7884627.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="JNX27_003569"
     CDS             4453..4761
                     /locus_tag="JNX27_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7884628.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="JNX27_003570"
     CDS             complement(4803..4979)
                     /locus_tag="JNX27_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7884629.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="JNX27_003571"
     CDS             complement(4976..5386)
                     /locus_tag="JNX27_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7884630.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="JNX27_003572"
     CDS             complement(5383..5661)
                     /locus_tag="JNX27_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884631.1"
                     /translation="MNDSYRQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..8084)
                     /locus_tag="JNX27_003573"
                     /pseudo
     CDS             complement(5736..8084)
                     /locus_tag="JNX27_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
BASE COUNT         1940 a         2229 c         1884 g         2138 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcgga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctgcc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gctttcgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc
     7981 gcatagctca tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc
     8041 agagcctgcc atccgaacag gcaaggttcg caatcacctt ctgaaccgcc ccgggtttcc
     8101 tggagagtgt tttatctgtg aactcaggct gccagatcat cgtttccgat ggaagcataa
     8161 taagcttttt ctgcttctgc cggagggatg t
//
LOCUS       DADLXX010000155         8158 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLXX010000155 DADLXX010000000
VERSION     DADLXX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.158"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(61..600)
                     /locus_tag="JNX27_003574"
     CDS             complement(61..600)
                     /locus_tag="JNX27_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7884632.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /locus_tag="JNX27_003575"
     CDS             complement(665..1213)
                     /locus_tag="JNX27_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7884633.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNX27_003576"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNX27_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7884634.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNX27_003577"
                     /pseudo
     CDS             complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNX27_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            4824..5540
                     /gene="nanC"
                     /locus_tag="JNX27_003578"
     CDS             4824..5540
                     /gene="nanC"
                     /locus_tag="JNX27_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7884635.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5560..6666
                     /locus_tag="JNX27_003579"
     CDS             5560..6666
                     /locus_tag="JNX27_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7884636.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6731..7711
                     /locus_tag="JNX27_003580"
     CDS             6731..7711
                     /locus_tag="JNX27_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7884637.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTTLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2425 a         1743 c         1699 g         2291 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata agtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatgtata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg
     3301 ctttaaggag tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct
     3361 tattcctcat cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
     3421 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg
     3481 taagtggacc agttcttctg agcgaactta aatggagtag cgatagttac ttataataac
     3541 tttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga
     3601 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
     3661 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttcaa
     3721 ctagccgtct caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca
     3781 tggaacgcag taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta
     3841 ttcctgcctg gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga
     3901 tatgggggaa cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc
     3961 actacgactt aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta
     4021 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag
     4081 tgaccagtta caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct
     4141 gatggcgagt aaacccttat tttcaccact ctcactgagt tagcttgcaa atctttcttt
     4201 gtttagattc taaataaatt atttataaaa catatagtta aaagaaaatc tttagttttt
     4261 gaagaccacc gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg
     4321 acatattttg tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga
     4381 tctggtataa caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat
     4441 ctatggcaaa catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga
     4501 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt
     4561 gagtaagtga tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac
     4621 tcaggtattt aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg
     4681 agaccttata caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg
     4741 caaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
     4801 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
     4861 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     4921 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     4981 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     5041 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
     5101 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     5161 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     5221 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     5281 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     5341 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     5401 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     5461 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     5521 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     5581 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     5641 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     5701 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     5761 gctgcattcc ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg
     5821 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     5881 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccgatgggc
     5941 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     6001 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     6061 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     6121 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     6181 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     6241 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     6301 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     6361 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     6421 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     6481 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     6541 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     6601 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     6661 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     6721 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     6781 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     6841 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     6901 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     6961 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     7021 gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc
     7081 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     7141 cggagccagc catgatgctt gtcgttgggg aacggatact acgctgtacc aggatttagt
     7201 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     7261 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     7321 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     7381 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     7441 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     7501 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     7561 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     7621 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     7681 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     7741 tgttcgttct cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt
     7801 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     7861 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     7921 ccttttcgat tatggctcgg aaatctacga acatctacac agtttactgg aacgttttgg
     7981 gccacagcgt tcgcagcacc gtcctgatat gccgttctgg cgattataag tcgacggatt
     8041 ctggcaaata gacgatgatg atatgcttaa tgatgttcaa agaaattagg ccattattat
     8101 attattgctt gcattttctt tcattcctaa aagcacccgc cattaatccc aggcgcat
//
LOCUS       DADLXX010000156         7875 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLXX010000156 DADLXX010000000
VERSION     DADLXX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.159"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            163..648
                     /locus_tag="JNX27_003581"
     CDS             163..648
                     /locus_tag="JNX27_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884638.1"
                     /translation="MRVIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSADLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELRERYLKNATITLNVIMPETKFTLLNVT
                     K"
     gene            645..1031
                     /locus_tag="JNX27_003582"
     CDS             645..1031
                     /locus_tag="JNX27_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884639.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGDYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LACWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3268
                     /gene="tssI"
                     /locus_tag="JNX27_003583"
                     /pseudo
     CDS             1056..3268
                     /gene="tssI"
                     /locus_tag="JNX27_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3291..3735
                     /locus_tag="JNX27_003584"
                     /pseudo
     CDS             3291..3735
                     /locus_tag="JNX27_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3758..4165
                     /locus_tag="JNX27_003585"
     CDS             3758..4165
                     /locus_tag="JNX27_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7884640.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4162..6640
                     /locus_tag="JNX27_003586"
                     /pseudo
     CDS             4162..6640
                     /locus_tag="JNX27_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            6637..7495
                     /locus_tag="JNX27_003587"
                     /pseudo
     CDS             6637..7495
                     /locus_tag="JNX27_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            7595..7798
                     /locus_tag="JNX27_003588"
     CDS             7595..7798
                     /locus_tag="JNX27_003588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884641.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
BASE COUNT         2013 a         1821 c         2298 g         1743 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagt gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg acctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct tcgggaacga taccttaaaa acgcaactat aacgttgaat
      601 gtcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga ttattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctggcatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggcagcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcggc ggcgtcgctt gcagccggtg gtccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gggccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgcag gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac
     2281 gttgatgaat cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct
     2341 gtttttacgg gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac
     2401 gtttgaactg ccccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca
     2461 caccaaaggc ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga
     2521 taaatatggc cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa
     2581 cagttcctgc tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca
     2641 gatcccgcgt attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc
     2701 catcatcgtg gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat
     2761 ggcgtcgcag agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat
     2821 gctgcgcttc gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct
     2881 caataccacg gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat
     2941 tgtcaaaaat gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga
     3001 tggtctttcc gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt
     3061 tggcgatcaa tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc
     3121 gattgagttt tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg
     3181 cagtggcatt gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc
     3241 gttacctacc cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc
     3301 tttaccgtat tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca
     3361 atatcttcgt cccatcgggc aatgaaaggg caactcccag tctgaatatt tcccgcgata
     3421 cgctcaggcc agatgaaagt ctgaccacct atattgaccg ccagatagcc ttgatgaaaa
     3481 aaaaacctgg gccagcaccg ggtactttcg cggggaacgg cactggcggg aactggcgaa
     3541 gctgccatga caggtgaaca gattctcgca actcatcgtt ctggaaaaac agaagtgtac
     3601 cagcgtcagg ccgggtttat caccgggcca gcaaaggtac taatgttgac gctgaccact
     3661 cagcgtcctt ttgatgatca caccgaccag ctatggactg catggttgac tagttttcag
     3721 cctgctaaaa gctaaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga
     3781 tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat
     3841 cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat
     3901 tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg
     3961 gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa
     4021 cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga
     4081 aatccgcatt gcccagggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat
     4141 gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc
     4201 ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg
     4261 gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg
     4321 gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg
     4381 gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc
     4441 actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc
     4501 acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg
     4561 cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta
     4621 cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac
     4681 ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc
     4741 gaccgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt
     4801 atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg
     4861 gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa
     4921 cgcctgtcgc atattgaact tgtgaagggc gggcgtcccg gtaacctggt ggaatatcgt
     4981 tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag
     5041 tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc
     5101 taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc
     5161 gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg
     5221 gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg
     5281 ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc
     5341 cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg
     5401 gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc
     5461 cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata
     5521 cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgaaca ggggctggcg
     5581 gtcagccgca cggatgcgcg gggcggcgag gttgtgctgg cgcacgatgc gcgcggtcaa
     5641 ctgaggcgtt acaccgactg ttcagggtgc gccacgggtt atgaatacga tgaagacggc
     5701 aacctgacgg cggtgacgga cgcggagggg aaaacggtac gcatcagcta taaccgtcag
     5761 gggctgccgg agacggtctg ccaccccggt aaacagcagg accgttacac ctggaacgcg
     5821 ctggggttac tgagcagcca ccgtcgcatc acgggcagtg tgcagtcctg gcagtatacg
     5881 ccgcgcggga tgctggcgcg ccatacggat gaagagaaac gggagacgcg ctggcagtac
     5941 accccggaag ggctggttgc cagtctgagc aacggcaacg gggctcagta ccggttcagc
     6001 tacgacggcg acggacggct gacgggagaa cagcgcccgg acggcctgat aaggatgttt
     6061 gcgctgaatg cggacgggtt tccggtcatc atccagacgc agggcacaga gggcggggtg
     6121 cgcaatgaac agcaggagcg tgatgcgctg ggccgcctgc tgcgcagtga cacgcagcac
     6181 agtacacgga ccttcagcta caaccgtctt gaccagataa cggaagtgac gctcaccccc
     6241 acggaagaag gggaacggct gcaccacatg caggcggaca cggtgcgttt tgcgtatgac
     6301 cgcagcgggt ggctgacggc agagcacagc gtgcacggca gtatcaaata ccggcgcgat
     6361 gcgctgggca atcccaccga tatcacgttg ccggacgggc agcatctcag tcatctttac
     6421 tacggcagcg ggcatctgct ccagacggca ctggacggta tcacggtcag tgaatatgag
     6481 cgtgacagcc tgcaccgtca ggtgatacgc acgcagggga aactggcgac gttcagcggc
     6541 tacaacgccg acaaccggtt aagctggcag cgcagcctgc caggtggcag ccagaatcca
     6601 cagcaggccg gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg
     6661 aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc
     6721 tatgaccgca gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat
     6781 tattacgaca aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac
     6841 cgcctgccgg gctacgggcg tgaccgttac cgctataacg aatggggcga actgacagag
     6901 cgcagggacc agcaactgga gtggaacgcg cagggccagt taacgcgggt catcagcagc
     6961 aacagcgata cgctgtatca gtatgatgcg ctgggcaggc gcatcagcaa ggtgacatat
     7021 ggccgccatc acggggcgcg gagagagcca gaaaatctgg tattaccaca cggacctgac
     7081 gggcacggta cacgaggtaa ccgcgccgga cgggacgctg gtgtgggcgg gctaccaggc
     7141 gggatttggt gaaaaccggg gcgacatcag caacagcggg gcgtactttg agcagccgca
     7201 gcggctgccg gggcagtact ttgacgaaga aacagggctg cattacaatc tgttcaggta
     7261 ttatgcgccg gagtgcggga ggttcgtaag ccaggacccg ataggattaa acggtgggtt
     7321 gaacttatat gcttatgcgc ctaatccgtt aggttggatc gatccgctag gactgaattc
     7381 tttaggaata gattcaaata atatatttag aggagatggt aactataaag gtggcggaat
     7441 tggacagcca aagagtggca caatatcagc acaggacatt attgatcatc tctaagaaaa
     7501 aggtgatggg cgtttaacat cttttagtac taaaaaaaca tctagttcca actctcgtgg
     7561 tgcagatttt ttctcaaaaa accatgtctt caaagtggca gtttctgatc tcgatgattt
     7621 agttaaaaat aatcagatca ctgttttaac acaagaaaaa gtaaaagcaa tatttggttt
     7681 agaatcaagc aagaaaatta gaaaagaact tggtaatatt cttcagaata tggctaggaa
     7741 taatgaggtg ttagttcatg ggcaaattct tggaaataaa gttagccgat gtaattagtt
     7801 agtggagagg aagttgtgga cgaaattaga ataagaattg ctggtaatac ggctgcgcct
     7861 tgtttccatg tgcta
//
LOCUS       DADLXX010000157         7819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLXX010000157 DADLXX010000000
VERSION     DADLXX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.160"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            211..429
                     /locus_tag="JNX27_003589"
     CDS             211..429
                     /locus_tag="JNX27_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7884642.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            647..889
                     /locus_tag="JNX27_003590"
     CDS             647..889
                     /locus_tag="JNX27_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884643.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVLGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNX27_003591"
     CDS             complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNX27_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7884644.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNX27_003592"
     CDS             complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNX27_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7884645.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2777..3679)
                     /gene="fdxH"
                     /locus_tag="JNX27_003593"
     CDS             complement(2777..3679)
                     /gene="fdxH"
                     /locus_tag="JNX27_003593"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7884646.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNX27_003594"
     CDS             complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNX27_003594"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6155..6157),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7884647.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6936..7769
                     /gene="fdhD"
                     /locus_tag="JNX27_003595"
     CDS             6936..7769
                     /gene="fdhD"
                     /locus_tag="JNX27_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7884648.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1814 a         2013 c         2076 g         1916 t
ORIGIN      
        1 aatataatat attcatagca cccataactc atcctgggcg ataactaaac catttttgag
       61 taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg caaaaacaaa
      121 atgacatgca aaaatccgca tttttttaaa tgctttttcg gcgtaatttt cgcacataca
      181 ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta
      241 tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct
      301 gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc ggtgcgtaaa
      361 gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat
      421 aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat
      481 taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc gtatctggaa
      541 aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcggcaagg
      601 gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga actctctggc
      661 aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta agcaacttga
      721 tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag cggtagatca
      781 atacctgata aatcaatcgc aaacagcaag aaccagtgtt cttggcatct ggcaagggtg
      841 tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat gggaaaaaat
      901 gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgtgaacgcc
      961 ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata
     1021 agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata accttatcga
     1081 tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg
     1141 cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca
     1201 cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt taatggaact
     1261 acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc
     1321 cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc
     1381 atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt
     1441 gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag
     1501 gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg
     1561 catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc
     1621 ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc
     1681 tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca tatcttccag
     1741 ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc
     1801 aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat cacgcggcag
     1861 aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc gcgcggtcag
     1921 atccatctcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga taagcgcagc
     1981 aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg
     2041 gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat tcgccgtacg
     2101 tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt
     2161 tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg
     2221 gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata
     2281 tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat
     2341 cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc
     2401 accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta
     2461 taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa
     2521 aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat gatgatgaac
     2581 gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg cggtgtgccc
     2641 atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc
     2701 aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg
     2761 atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt
     2821 cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc gcaaaggtag
     2881 ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc
     2941 tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca
     3001 cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac
     3061 cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac
     3121 cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc ccaaccacca
     3181 cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg aggcgcggaa
     3241 tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg gactggaagt
     3301 cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct ttcaggcagc
     3361 ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct
     3421 gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca ttggggttgt
     3481 cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca
     3541 cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc acttcttcct
     3601 ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat
     3661 cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg
     3721 acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa gtattggcaa
     3781 taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg atggtatcga
     3841 tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct ttggctttga
     3901 tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca agtttattcg
     3961 ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc aacagcgcgt
     4021 gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact
     4081 tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga
     4141 taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg
     4201 ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca
     4261 tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga atatcccagc
     4321 cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac gggttaccct
     4381 gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc
     4441 agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc
     4501 cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc
     4561 gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg
     4621 gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct tccgacttcg
     4681 gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg ttcgcgccac
     4741 cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcaccgtcag
     4801 tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cgacctgagt
     4861 taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac acttcggtct
     4921 ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag ttagaggttt
     4981 cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa ccgatcactt
     5041 tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct
     5101 tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca
     5161 accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag gccttcatca
     5221 tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc agcagcggtt
     5281 ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt ggcagcgtca
     5341 tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga atattggagt
     5401 gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc agcagctgga
     5461 tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag ccgagggcat
     5521 acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg cagactttca
     5581 ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg tagcgggaaa
     5641 cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg
     5701 cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg cgtttttccg
     5761 cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg
     5821 cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc aggtacagca
     5881 atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag tagtcagcta
     5941 ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt
     6001 tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca
     6061 ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa
     6121 atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg
     6181 cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg ttactcgacg
     6241 cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct tcggcgtttt
     6301 gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct
     6361 cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc ggaaacttca
     6421 gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa agtgcaccgc
     6481 ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg gcgttttttg
     6541 ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag gtgcaggtat
     6601 tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt gctacgctgg
     6661 gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag atcttaaaga
     6721 actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac gtcccattta
     6781 cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa ggggccgcaa
     6841 aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag aataccataa
     6901 tgttggtgtg tgtgttctta tctggttaag agaaagtgaa aaaaacacag cgaaaagaaa
     6961 tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg cgtgatgatt
     7021 tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg
     7081 gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt
     7141 tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc
     7201 cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt atggggttga
     7261 aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac
     7321 ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt gatctcaaca
     7381 aactggatga tgcattacgt catctcaacg attttcagcc agtggggcaa ctgactggtt
     7441 gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg catgaagacg
     7501 tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa ggggaaagct
     7561 ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg
     7621 cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag
     7681 tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg gcaacggttt
     7741 atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat atattaaatt
     7801 gcaggactta ttcatttcg
//
LOCUS       DADLXX010000158         7561 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLXX010000158 DADLXX010000000
VERSION     DADLXX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.161"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1675
                     /gene="cpdB"
                     /locus_tag="JNX27_003596"
     CDS             <1..1675
                     /gene="cpdB"
                     /locus_tag="JNX27_003596"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY7884649.1"
                     /translation="GLKAGDVHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGAK
                     FPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMGWDKANL
                     SGKVTMNDITETVRKYVPEMREKGADVIVVLAHSGLSADPYKVMAENSVYYLSEIPGV
                     NAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGK
                     WQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYSY
                     LALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPASYVEV
                     EKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIAPNSTKPQSLINWD
                     GFRTYNFDVIDGVNYQIDVTQPARYDGECQMINVNAERIKNLTFNGKPIDPNAMFLVA
                     TNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPI
                     AADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(1801..2181)
                     /locus_tag="JNX27_003597"
     CDS             complement(1801..2181)
                     /locus_tag="JNX27_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7884650.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2270..3130
                     /gene="qorB"
                     /locus_tag="JNX27_003598"
     CDS             2270..3130
                     /gene="qorB"
                     /locus_tag="JNX27_003598"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7884651.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            3239..4204
                     /locus_tag="JNX27_003599"
     CDS             3239..4204
                     /locus_tag="JNX27_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7884652.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLSQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            4312..4974
                     /gene="ytfE"
                     /locus_tag="JNX27_003600"
     CDS             4312..4974
                     /gene="ytfE"
                     /locus_tag="JNX27_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7884653.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5019..6431)
                     /gene="cycA"
                     /locus_tag="JNX27_003601"
                     /gene_synonym="dagA"
     CDS             complement(5019..6431)
                     /gene="cycA"
                     /locus_tag="JNX27_003601"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7884654.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(6739..7359)
                     /locus_tag="JNX27_003602"
     CDS             complement(6739..7359)
                     /locus_tag="JNX27_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7884655.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1942 a         1975 c         1928 g         1716 t
ORIGIN      
        1 aggattaaaa gcaggtgatg ttcatccggt ttataaggcg ctgaatacgc tggattatac
       61 ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg gattacctga aaaatgcgct
      121 ggcgggagcg aaattccctt atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc
      181 aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca
      241 gacgctgaag attggctata ttggcgtcgt gccgccgcaa atcatgggct gggataaagc
      301 taatttatcc ggaaaagtga cgatgaatga tattaccgaa accgtgcgca aatacgtgcc
      361 tgaaatgcgc gagaaaggtg ccgatgtcat tgtcgttctg gcgcattccg ggctgtctgc
      421 cgatccgtat aaagtaatgg cggaaaactc agtttattac ctcagtgaaa ttccgggcgt
      481 taacgccatt atgtttggcc atgctcacgc cgttttcccg ggtaaagatt ttgctgatat
      541 cgaaggggct gatatcacca aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat
      601 gtggggcgat catcttgggg tggtcgactt acaactcagt aatgacagcg gtaaatggca
      661 ggtgacgcag gcgaaagcgg aagcacggcc gatttacgac atcgccaata aaaaatccct
      721 cgcggcggaa gacagcaagc tggtagaaac actcaaagcc gatcacgatg ccacacgcca
      781 gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg tatagctatc tggcgctggt
      841 gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta
      901 cattcagggc gatccggatc tggcaaaact gccggtgctt tcagctgccg caccgtttaa
      961 agttggtggt cgcaaaaatg acccggcaag ctatgtggag gtggaaaaag gccagttgac
     1021 cttccgtaat gccgccgatc tttatctcta ccccaatacg ctgattgtag tgaaagccag
     1081 cggtaaagag gtgaaagagt ggctggagtg ctccgccgga cagtttaacc agattgctcc
     1141 taacagcacg aaaccacagt cactcatcaa ctgggatggt ttccgcactt ataactttga
     1201 tgttattgat ggtgtgaatt atcagattga tgttacccaa cccgcccgct atgacggcga
     1261 gtgccagatg attaatgtca atgcagagcg gattaagaac ctgaccttta atggcaagcc
     1321 gattgatccg aacgctatgt tcctggttgc caccaataac tatcgcgctt acggcggcaa
     1381 atttgccggt acgggcgaca gccatatcgc ttttgcttca ccggatgaga accgctcggt
     1441 gctggcagcg tggattgctg atgagtcgaa acgtgcgggg gaaattcatc cggcggcaga
     1501 taacaactgg cgtttagcac cgatagctgc cgataagaaa ctggatatcc gtttcgaaac
     1561 ttccccgtca gataaagccg cagcgtttat taaagagaaa gggcagtatc cgatgaataa
     1621 agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg gatttgagta agtaaaacac
     1681 ttctttttcg gcctataaat caaccgcatc cggcatttat tggcggatgc gatgctgccg
     1741 tatcttatcc accctacaag ccatgaaccg tagaccggat aagcgcagcg catccggcag
     1801 ttatgccgca cgttcatccc gcaccgccaa cacctcgggc aaattcaact caatccagtc
     1861 cgccagtgca gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc tatattccac
     1921 atgcggcggc accaccggat acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg
     1981 taacgactgc gcaagcatct tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa
     2041 gcgatgagta ccttcgcgta gcgccaccag aatcaacacc ccccaacggc tggtgacgtg
     2101 tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga ttaccctctt tcagttgttg
     2161 tgacaggcta acctgactca tttcatactt acctttttgc atgtacttac taaaagtaag
     2221 tttaggtgtt agcgtattta aacacaagac aaaacgatgg agacttccca tgatcgctat
     2281 tactggtgcc actggccaac ttggtcacta tattattgaa tccttgatga aaacggttcc
     2341 tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca
     2401 aggcattacc gtgcgtcagg ctgactacag cgatgaagcc gcactgacat ctgcacttca
     2461 gggagtggaa aaactactgc tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca
     2521 tcgtaatgtt attaatgccg caaaggcggc tggcgtgaaa tttatcgctt ataccagcct
     2581 gctacatgca gatacctccc cgctcggcct cgccgatgag cacatcgaga cggagaaaat
     2641 gctggctgat tctggcatcg tttacaccct gctgcgcaac ggctggtaca gcgaaaacta
     2701 cctcgccagc gccccggcag cactggaaca cggcgtattt atcggtgcgg cgggcgatgg
     2761 caaaatcgcc tcagcaacgc gggcagatta tgcgacagct gcggcactcg tgattagcga
     2821 agccggtcac gaaggcaagg tttacgaact ggcgggcgat agtgcctgga cgttgacaca
     2881 gttagcggca gagctgacca aacagagcgg caaacaggtt acctatcaaa atctgagcga
     2941 agccgatttc gccgcggcac tgaaaagcgt cggactgccc gacggactgg cggatatgct
     3001 ggcggattct gacgttggcg catcgaaagg tggtctgttt gataacagca aaacgcttag
     3061 caaattgatt gcccgcccaa cgacaacgtt agccgaaagc gtaagccatc tttttaatgt
     3121 taataactag ttaattaaag tggcatcctc ccgcatcctc tctgataatg acgggatgcc
     3181 gggagcaatc atgtctgctt cctgaacgtt tcttctgaca gaccaatgga tgccagtaat
     3241 gattagcggc gtgctgtacg ccctgttagc agggttgatg tgggggctta tttttgtcgg
     3301 gccgttgatc gtgccggaat acccggcgat gttacagtcg atggggcgtt atctggcgtt
     3361 agggttaatt gcgctaccca ttgcctggct gggacgcgtg cgtctgcgtc agttggcgcg
     3421 tcgggactgg cttaccgcct tgatgctcac tatgatgggc aacctcattt attacttctg
     3481 ccttgccagt gccattcaac gtactggcgc gcctgtttcc acgatgatta tcggcaccct
     3541 gccggtggtg attcctgtct ttgccaatct gctttatagc cagcgcgacg gcaaactcgc
     3601 gtggggaaaa ctcgccccgg cactgatttg tattggcatc ggcctggcga gtgtgaatat
     3661 tgctgagtta aaccacggac tccccgattt tgactgggca cgttatacct ctggcatcgt
     3721 gctagcgtta gtttccgtgg tctgctgggc atggtatgcc ctgcgcaacg cccgctggct
     3781 gcgggaaaat cccgacaaac atccgatgat gtgggcgacg gcgcaggcgc tggtcacgct
     3841 gccggtttct ctcatcggct atctcgtcgc ctgttactgg ctgaatatac aaacgccgga
     3901 cttctcctta ccttttggcc cccgtccgct ggtgtttatt agtctgatgg ttgcgatagc
     3961 cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc gccagccagc gattaccgac
     4021 agtgattctc gggccgctga ttgttttcga aacgctggca ggtttgctgt acaccttttt
     4081 actcagccag caaatgccgc cgctaatgac gctgagcggt atcgcgctgt tagtgattgg
     4141 cgtggtcatc gcggtcagag caaaaccaga aaaaccttta actgaatctg tctcagaaag
     4201 ttgacatgct ggcagtgagt taaataagcc tctgctacgt aagggttata gcttttgcct
     4261 taaaggtgta tttaaaatac atcttatctt attaagaatg aggtatcagc tatggcttat
     4321 cgcgaccaac ctttaggtga actggcgctc tctatccctc gcgcttcggc tctgtttcgt
     4381 aaatatgata tggattactg ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt
     4441 aaagaactgg atgttgaggt cattgaagct gaactggcaa agcttgctga acaaccgatt
     4501 gagaaagact ggcgtagcgc cccgctggca gaaatcattg accatatcat cgtgcgctac
     4561 cacgatcgtc accgcgagca gctgccggag ctgatcctgc aagcgactaa agtcgagcgc
     4621 gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa aatacctgac catgctgcat
     4681 gaagagcttt ccagccacat gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa
     4741 ggcatgggca gccaggcaat ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg
     4801 ggcgaactgc tggaagtgat caaacacact accaataacg tcacaccgcc gccagaagcc
     4861 tgcaccacct ggaaagcgat gtataacggc attaatgaac tgattgatga cctgatggat
     4921 cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc tagcgggtga gtgatctaaa
     4981 agctggatgg cattgcgcca tccagcatga taatgcggtt atttccgcag ttcagcagcc
     5041 cgcttcttac caataaacag ccagcccaac cccagcgcga taaaccacag cggggtaacc
     5101 agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa cacaaagaac
     5161 gccatacata cccagcacat cagcttgccg agcggcatct tgtagatcga cttttcatgc
     5221 agatgaggac gctgtttgcg gtacaccagg tacgagcaaa ggataatcgt ccagacgaac
     5281 ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac gctaggattc
     5341 acatacaaca tcaccacgcc gccgagcaga cagatacacg agaaggtcaa ccctttcgcg
     5401 ggtactgcgc gcttagaaag tttagcgaac gctttcggcg caacaccttc ctgcgccaga
     5461 ccaaacagca tacggctggt agagaagaca ccgctgttag cggaagacgc cgcagaggtc
     5521 agcaccacaa agttgatcac gctggcagcc gcaggcaaac ctaccaacac aaacagctca
     5581 acgaacgggc ttttctccgg gactaccgaa ctccacggtg tcacggacat aatcacaatc
     5641 agcgcgaaga cgtagaacat aatgatacgg atcggaatgg agttaatcgc gcgtggcagt
     5701 gatttctccg gatctttggt ttccgcagct gttgtaccta ccagctcaat ccccacgaaa
     5761 gcgaaaaccg ctatctggaa tccggcaaag aagccactta aacctttcgg gaaccagccg
     5821 ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg tcggtgactg aaagtgcatc
     5881 gccaccatga ccaggccgac gacaatcagc gacacgatgg cgacgatttt gatcatcgca
     5941 aaccagaact ccatctcacc gaacattttc acggtggcaa ggttgagcgt cagcagcagc
     6001 actatcaccg ccagcgaggc gacccagtcg gagaaatcgg ggaaccagaa ctgagcataa
     6061 gccgtgatcg ccaccacgtc tgccataccg gttacaaccc agcagaacca gtaagtccag
     6121 ccggtgaaat atcccgccca cggccccagt aaatcggaag cgaagtcact aaaagatttg
     6181 tattccagat tcgaaagcag caattccccc attgcccgca tcacgaaaaa gagcataaaa
     6241 ccaatgatca tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt tttgccggac
     6301 cccataaaca acccagtacc aatggcaccg ccaatggcaa taagctgaat atgtcggttt
     6361 gtgagattgc gccgtagcga ctgttcagcc ggagcctgat catcggcaac gacttttacc
     6421 tgatctacca tgttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta
     6481 gtcagtctat gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg
     6541 ggatgaatac taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca
     6601 cggtgataac gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa
     6661 tagcgccgtt tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag
     6721 gggaacagaa tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct
     6781 gaacggaggg atggatgcac ctgcgccgcg ctcgccatat gccagttcct gcgggatagt
     6841 cagttcccat ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac
     6901 gccattaacc gggaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc
     6961 gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc
     7021 ttcaccctgg ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt
     7081 tttggcgttt tcttccaggt atttcacacc ttcagcagcc atcgcctgga aacgctgacg
     7141 acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg cgatgcacca catcaaccgg
     7201 cacagccgga tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag
     7261 cagcccttcc aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct
     7321 tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta
     7381 taaaagtagc gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg
     7441 atgacaaatc ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg
     7501 ggttggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa ggtgcttgcg
     7561 t
//
LOCUS       DADLXX010000159         7462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLXX010000159 DADLXX010000000
VERSION     DADLXX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.162"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            215..291
                     /locus_tag="JNX27_003603"
     tRNA            215..291
                     /locus_tag="JNX27_003603"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /locus_tag="JNX27_003604"
     CDS             complement(337..1098)
                     /locus_tag="JNX27_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7884656.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="JNX27_003605"
     CDS             complement(1281..2171)
                     /locus_tag="JNX27_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY7884657.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNX27_003606"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNX27_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY7884658.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKDPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="JNX27_003607"
                     /pseudo
     CDS             complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="JNX27_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
BASE COUNT         1738 a         1939 c         1952 g         1833 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggatct
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagctg ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg cgccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgtca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc cgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agctgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt atgaattgtg
     7441 acagcttttt accattaata gg
//
LOCUS       DADLXX010000160         7401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLXX010000160 DADLXX010000000
VERSION     DADLXX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.163"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..233)
                     /locus_tag="JNX27_003608"
     CDS             complement(<1..233)
                     /locus_tag="JNX27_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884659.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="JNX27_003609"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="JNX27_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7884660.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="JNX27_003610"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="JNX27_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7884661.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="JNX27_003611"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="JNX27_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7884662.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="JNX27_003612"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="JNX27_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="JNX27_003613"
     CDS             complement(3305..3424)
                     /locus_tag="JNX27_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7884663.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="JNX27_003614"
     CDS             complement(3385..3570)
                     /locus_tag="JNX27_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884664.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="JNX27_003615"
     CDS             3671..3844
                     /locus_tag="JNX27_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884665.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3906..4190
                     /locus_tag="JNX27_003616"
     CDS             3906..4190
                     /locus_tag="JNX27_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7884666.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="JNX27_003617"
     CDS             4194..4529
                     /locus_tag="JNX27_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7884667.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="JNX27_003618"
     CDS             complement(4586..6907)
                     /locus_tag="JNX27_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7884668.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTETTNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="JNX27_003619"
     CDS             complement(7024..7233)
                     /locus_tag="JNX27_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884669.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2032 a         1742 c         1647 g         1980 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtatggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcagc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtaggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgttt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac c
//
LOCUS       DADLXX010000161         7297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLXX010000161 DADLXX010000000
VERSION     DADLXX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.164"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JNX27_003620"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JNX27_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY7884670.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="JNX27_003621"
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="JNX27_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7884671.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="JNX27_003622"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="JNX27_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="JNX27_003623"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="JNX27_003623"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7884672.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="JNX27_003624"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="JNX27_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7884673.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /locus_tag="JNX27_003625"
     CDS             complement(5042..6469)
                     /locus_tag="JNX27_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7884674.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="JNX27_003626"
     CDS             complement(6492..7184)
                     /locus_tag="JNX27_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7884675.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1845 a         1764 c         1929 g         1759 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacagccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt tgatggcaca ttaacgggac ttttgct
//
LOCUS       DADLXX010000162         7275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLXX010000162 DADLXX010000000
VERSION     DADLXX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.165"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            91..432
                     /gene="yneK"
                     /locus_tag="JNX27_003627"
                     /pseudo
     CDS             91..432
                     /gene="yneK"
                     /locus_tag="JNX27_003627"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            462..1211
                     /gene="yneK"
                     /locus_tag="JNX27_003628"
     CDS             462..1211
                     /gene="yneK"
                     /locus_tag="JNX27_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7884676.1"
                     /translation="MFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLSLLAEQESPHFQD
                     LFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKV
                     THEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            1361..2551
                     /gene="ydeA"
                     /locus_tag="JNX27_003629"
     CDS             1361..2551
                     /gene="ydeA"
                     /locus_tag="JNX27_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7884677.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2576..3241)
                     /gene="marC"
                     /locus_tag="JNX27_003630"
     CDS             complement(2576..3241)
                     /gene="marC"
                     /locus_tag="JNX27_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY7884678.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3453..3887
                     /gene="marR"
                     /locus_tag="JNX27_003631"
     CDS             3453..3887
                     /gene="marR"
                     /locus_tag="JNX27_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7884679.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3907..4290
                     /gene="marA"
                     /locus_tag="JNX27_003632"
     CDS             3907..4290
                     /gene="marA"
                     /locus_tag="JNX27_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY7884680.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4322..4540
                     /gene="marB"
                     /locus_tag="JNX27_003633"
     CDS             4322..4540
                     /gene="marB"
                     /locus_tag="JNX27_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7884681.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="JNX27_003634"
     CDS             complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="JNX27_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY7884682.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5734..6851
                     /gene="ydeE"
                     /locus_tag="JNX27_003635"
                     /pseudo
     CDS             5734..6851
                     /gene="ydeE"
                     /locus_tag="JNX27_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6978..7073
                     /gene="mgtS"
                     /locus_tag="JNX27_003636"
     CDS             6978..7073
                     /gene="mgtS"
                     /locus_tag="JNX27_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY7884683.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1897 a         1773 c         1652 g         1953 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tatccctttt agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca
      961 atatgttatt gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt
     1021 tgatgccagc atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca
     1081 aactgtttgt aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca
     1141 ggcctcgctc ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg
     1201 aacgtcatta aaccgtgatg ttaccgactc tctgacgcgg aacagaatca gcgtcagaga
     1261 aacggaaaac gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga
     1321 tcatttcatc actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc
     1381 ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac
     1441 caccgaattt gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac
     1501 cgctcaggtc ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc
     1561 ttttatgtta atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt
     1621 gtttattgcc agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag
     1681 tcgcattggt gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat
     1741 ccgtatggct ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc
     1801 actggcgatg gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg
     1861 aatgaccttc ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt
     1921 acttccctta ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg
     1981 ccgcccggca ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac
     2041 ggcatacagc tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc
     2101 cacggcatta ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa
     2161 actgggtaat caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg
     2221 tctggcactg ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt
     2281 ctgggggatc gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc
     2341 accagatgct accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg
     2401 ggcgggtgcg ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta
     2461 tgtgggcgcg gtgcctgctt ttgccgcgtt aatatggtca atcattatat ttcgccgctg
     2521 gccagtgaca ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat
     2581 ggtacgtttt aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca
     2641 gcaggaaccc catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa
     2701 ttgcgccgga gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg
     2761 gcggcgcaac catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg
     2821 agctaataat catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa
     2881 aggcaatatt ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg
     2941 aatcaatcgc tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc
     3001 cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag
     3061 cgtaatacgc caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat
     3121 tacgttcggc actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg
     3181 ggttagctaa cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca
     3241 ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg
     3301 ataaagtgtc actctttagc taaccttgca tcgcattgac caaaacttga accgatttag
     3361 caaaacgtgg catcggtcaa ttcattcatt tgacttatac ttgcctaggc aatattatcc
     3421 cctgcaacta attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg
     3481 aaattattcc attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta
     3541 acgagtatct gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc
     3601 gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag
     3661 cactgacccg tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc
     3721 cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca atatgcgaac
     3781 aatgccatca attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg
     3841 acgaagttgc aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt
     3901 atgacgatgt ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc
     3961 gaggacaacc tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc
     4021 aaatggcacc tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc
     4081 cgcagccgta agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc
     4141 tatctggcag aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat
     4201 tactttgatg ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt
     4261 ttacatccat taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat
     4321 catgaaacca ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt
     4381 tgcggaacaa accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc
     4441 atcgcaggaa caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga
     4501 tgcgctcggc gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc
     4561 tcggggctta ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga
     4621 tatacagccc ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat
     4681 ccaacaatag tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa
     4741 cccgccaggt ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg
     4801 ccacaaacgc cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt
     4861 gaatcatgga tgcggaacca tcgagaatca gcgaggcaac aaagaaggga atgattggga
     4921 ttaaagcgct ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct
     4981 ttttattgaa gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc
     5041 cgagcatcgc cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa
     5101 tcgctaaggc gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc
     5161 caagcacgat agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca
     5221 taccaaagtt aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat
     5281 accccaagag caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag
     5341 cgaccagcat aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt
     5401 tgatgaccac aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt
     5461 ttcgcgacat tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt
     5521 tacttttctt taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata
     5581 agtcgctata cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag
     5641 aagggcgctg gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg
     5701 ttatgtttta ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt
     5761 tacttgagtc gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg
     5821 ctcactattg gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag
     5881 aaacgctata tgttactggc aattaccgcc ttcccagcgg ttttattgcc attcctttag
     5941 tgaataacgt gacgctggtt gtgctctttt ttgccctcat taactgcgcc tattctgttt
     6001 ttgctaccgt gctgaaagcc tggtttgccg acaatctttc gtccaccagc aaaacgaaaa
     6061 tcttctcaat caactacacc atgctaaaca ttggctggac catcggtccg ccgctcggca
     6121 cgctgttggt gatgcagagc atcaatctgc ccttttggct ggcagctatc tgttccgcgt
     6181 ttcccatgct tttcattcaa atatgggtaa agcgcagcga gaaaatcatc gccacggaaa
     6241 caggcagtgt ctggtcgccg aaagttttat tacaagataa agcactgttg tggtttacct
     6301 gctctggttt tctggcttct tttgtaagcg gcgcatttgc ttcatgcatt tcacaatatg
     6361 tgatggtgat tgctgatggg gattttgccg aaaaggtggt cgcggttgtt cttccggtga
     6421 atgctgccat ggtggttacg ttgcaatatt ccgtgggccg ccgacttaac ccagctaaca
     6481 tccgcgcact gatgacagca ggcaccctct gtttcgtcat cggactggtc ggttttattt
     6541 tttccggcaa caacctgcta ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa
     6601 tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca ggaatgaaag
     6661 ccagctattt ttccgcccag tctttaggct ggcttggtgc cgcgattaac ccattagtga
     6721 gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt tgtcatctta gcgttggtga
     6781 tcattgctgc gtgggtgctg atgttaaaag ggattcgtgc aagaccgtgg gggcagcccg
     6841 cgctttgttg atttaagtcg aacacaataa aaatttaatt cagccttcgt ttaggttatc
     6901 tctgctaata tctttctcat cgagatgaaa attaaggtaa gcgaggaaac acaccacacc
     6961 ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta ctgggaataa
     7021 ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac taatgaacgg
     7081 agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc ctgattttta
     7141 tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg
     7201 ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc
     7261 atcagttttc tcaat
//
LOCUS       DADLXX010000163         7209 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLXX010000163 DADLXX010000000
VERSION     DADLXX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.166"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..331)
                     /locus_tag="JNX27_003637"
     CDS             complement(<1..331)
                     /locus_tag="JNX27_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884684.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="JNX27_003638"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="JNX27_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7884685.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="JNX27_003639"
     CDS             complement(1692..2072)
                     /locus_tag="JNX27_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY7884686.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="JNX27_003640"
     CDS             complement(2086..2502)
                     /locus_tag="JNX27_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884687.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYLSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JNX27_003641"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JNX27_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7884688.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="JNX27_003642"
     CDS             complement(3503..3970)
                     /locus_tag="JNX27_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7884689.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="JNX27_003643"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="JNX27_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7884690.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="JNX27_003644"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="JNX27_003644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7884691.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..>7209
                     /locus_tag="JNX27_003645"
     CDS             7030..>7209
                     /locus_tag="JNX27_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY7884692.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
BASE COUNT         1850 a         1766 c         1806 g         1787 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatacgcca ttataggtcc tggatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga ccttaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaga taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaatgaaaat atatatcggc gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagca cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gagaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aagggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctg
//
LOCUS       DADLXX010000164         7186 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLXX010000164 DADLXX010000000
VERSION     DADLXX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.167"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="JNX27_003646"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="JNX27_003646"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY7884693.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /locus_tag="JNX27_003647"
     CDS             complement(986..2344)
                     /locus_tag="JNX27_003647"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7884694.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLRELKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNX27_003648"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNX27_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7884695.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="JNX27_003649"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="JNX27_003649"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7884696.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="JNX27_003650"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="JNX27_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="JNX27_003651"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="JNX27_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="JNX27_003652"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="JNX27_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7884697.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2034 a         1626 c         1509 g         2017 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgacag
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgagctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacagc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cagcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt
     6481 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttat
//
LOCUS       DADLXX010000165         6838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLXX010000165 DADLXX010000000
VERSION     DADLXX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.168"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..232)
                     /locus_tag="JNX27_003653"
     CDS             complement(<1..232)
                     /locus_tag="JNX27_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7884698.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDE"
     gene            complement(300..3779)
                     /locus_tag="JNX27_003654"
                     /pseudo
     CDS             complement(300..3779)
                     /locus_tag="JNX27_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            complement(3840..4441)
                     /locus_tag="JNX27_003655"
                     /pseudo
     CDS             complement(3840..4441)
                     /locus_tag="JNX27_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(4378..5121)
                     /locus_tag="JNX27_003656"
     CDS             complement(4378..5121)
                     /locus_tag="JNX27_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884699.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            complement(5126..5824)
                     /locus_tag="JNX27_003657"
     CDS             complement(5126..5824)
                     /locus_tag="JNX27_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7884700.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            complement(5824..6153)
                     /locus_tag="JNX27_003658"
     CDS             complement(5824..6153)
                     /locus_tag="JNX27_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884701.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            complement(6153..6838)
                     /locus_tag="JNX27_003659"
                     /pseudo
     CDS             complement(6153..6838)
                     /locus_tag="JNX27_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         1459 a         2181 c         1641 g         1557 t
ORIGIN      
        1 gctcatcttc agcgttggca tagctgaatg acgtcaccag ccccagcgtg tccgtaaact
       61 cataacggta tttcacgtta atgcccttca ggtcatcgct gcctggcata tcagtatggg
      121 gctgaagata cccggcgctc agcgtggact gatgttcaga cgcccatgca ggcgcaccgg
      181 atacagccag acagatggct gcggacaaaa tggctgcaca aactttacgc ataattacct
      241 ctcgcttttc tgcaataaaa aaggcaccat ttctggtgcc cgtatctggg ttataaaatt
      301 cagctaatcg tgatgcctgc agtggctttc ttcatcacaa caaccagcaa atcgctgata
      361 cttgctgtgg gataccagcc atttaccagc catgccgata cagaaaactc cagcgtcatg
      421 tgaccgtgac cggcaggcat atcaataaca cccgtatata tcagcgtatt atccagggtc
      481 gttcggttat aaatttcagc accgtttttc ttcactatca ggcggcatga cgaataaata
      541 ttgttattct tccgctcatg tttagcaccg cgaaacgcca ccgcgggaat aacaatttgc
      601 cgatcaaacg gctgatcgtc ataaaccctg acggtaatgg tccctgatgg ccacctctcc
      661 ggtgcccggg agtcccgtgg gaaaggtttg cccactgttt taacgagatc gccttcaatc
      721 tggttcgcgg acaattttcc cagaacccga cagttcttat taatcgtgac gttgttgagc
      781 gtcccggagt tcgcgttcac gttaccgctg atatcggcat ttttcgccgt cagccgtccg
      841 ccaggtgtca gggaaaatgc cggaggatta ccgccgctgg taatggtcgg agccgtcagg
      901 cgtttcagga acacgtcgtt catgaatatc tgatcgctct gaccaacaaa catcggcttt
      961 gtgttgccat tcgcaggatt aatcatcgca atcctgtctg ccgccagcag cacctgactc
     1021 tgcattcctg ctggcgtatt ctcaataccg gcaccgatac ccgcaatata aaggcgtccg
     1081 tccttcatct gttgcagctt cacagcccac atgctgttca ggttattatt tgtatcaacc
     1141 tgaactttct gtatctgctg gattgcagca ctctgatttt ccagtgtttt attgacggtt
     1201 tgcgtgattt cattgctgac attcgtaatg gacgtcctga tttcagtcag gtccggcgca
     1261 agctgaccgt tatcaatctg cgtccacaac tccttgccga gatgcgtttt attgatcaac
     1321 cctttataaa aactcagata accttccgca taatcgctcg cccgaccgac ggcctccaca
     1381 aatgccgatt tgccaacggt gttcacactg cgaacgtaaa aataataatc atggcccggc
     1441 ctgatattga tactggcggc tatccagtac agcgccgtgc caagatagcg ggcgctggtt
     1501 tcaacctgcc tgatatccgc aatccgcttt tccgaaaacc agaactcaaa ctgcaccgtc
     1561 gggtcatata cagccagttt cgggaccacc gttatctgaa aataccccgg tatcagttca
     1621 atagtgacag gcgctgccgg tgccgcaatc cggaacgata ccgacgccgg atcgccctgc
     1681 tgcccccggg catttaccgc ccggactgtc agactgtaat tccccagcgc cagctgcctg
     1741 aagcggtatg tggtttccgt cgtcctggct gtgctgacca gccgctcact gccgtcgtcc
     1801 gctgccacgg tcaggcgaag caggaagctc acacccttca ccaccttcgg cgtgtcccat
     1861 cgctccagca cctgatattc cccgctgtct gcggtgactt cggcggtcag atgctgcacc
     1921 gctggcggcg tgacaccatt caccgtgccg cgccggtcac cgtcaaagtg tgccccgtta
     1981 tccacgatgg cctctttttc cggcacatgc tgcacggcgg tgatggcata cgtgccgtcg
     2041 tcgttctcac ggatactcac gcagcggaac aggcgctggc gcagcgtcgg cagcttcagc
     2101 ccccacacgc tgtattctgc aacgccgtca ggaacccggc tcactttcac cttcacgccg
     2161 tcggtgacgg actgaacctc cacgctgacc ggattgccat ttccgtcaac caggcttatc
     2221 agcgtggtac cggaggacgg cagcgtgatt tcacggtcga gcgtcagcgt ccgggtctgg
     2281 ctgttcaccg ccagtacgcg accaccggtg ctgataccgg cataatcatc atcgcagatt
     2341 tcaatgacat cacccggtac atggcgaagc ccttctgcgc cgacgctgaa gtccacggtc
     2401 tgcgtttcca gcagttccgt tttaatcagc cacagcccgg cgcggtgcgc ctgtccccgg
     2461 ctggtacagc caaaggcatc catcttcgtg acgttacgac cgtaacggac aatggcctgc
     2521 gtgtcctcca caagctctgt cgccgtctca tgaccgttgt ccggatcaat ccagttcacc
     2581 tcaacgacat tatggcggtc cttcagggcg ctgaagctgt agcggaacgg cgcgccatca
     2641 tccggcatca ccacattact gcggttatag gtccacactt tatccgacgg tcggtcctgc
     2701 acgaacgtca gcgtctgccc gttccatacc ggcatacagc gcatcgccga gcagaaatca
     2761 ctgagaacat cccacgcctt acgctgtgtg gtcaggtaag cgttacaggt gatgcgcggc
     2821 tccgtgccgc caaatccgtc cggcactgac tggtcgcagt actggccgat gacatacagc
     2881 gcccatttgt ccacatctgc cgcaccacga cgtttcccca tgccttagcg cggatgagtg
     2941 agcatgtccc acagacacca ggccatgttg ttgctgtatg ctggcttaag cgttccgtcc
     3001 cagataccgc tgtattgccg cgtctgcggg ttatagttcg acggcacctg cagaatgcgc
     3061 ccgcgaagat gataattgcg actcacctgc tggttaccga actgctctga atccacctgc
     3121 acgccgacca gcgccgtgtt cgggtagcac tgtttcacat cgatgatttc ggtgtacgac
     3181 gaccagagcg ttttgttctg cagctggtct gtggtgctgt ccggtgtcat cctacgcatc
     3241 cggatattaa acgggcgcgg cggcaggtta cccaccacca ccgaggccag atactgcgaa
     3301 gtggttttgc ccttaatggt gatgtctttt tccgtcaccc agccaccgtt acgctgtatc
     3361 tgaaccagca ggcggacttc cgacggattc cggtccccct ttgaggtggt ttccaccagt
     3421 gcctgcacac cgaaggtcag gcgcagacga tcaatgtttg ccgacgtgat ggtccgggtg
     3481 atcggcgtgt cgtatttcac ttccgtaccc agcacagtct ctgagccgga agattcaaac
     3541 ccctccggcg gtgtctgctc ctgctcgccg gcgcggaaca ccaccgtaac accggaaaag
     3601 tttgtcttcc cctcgctgtc caggaccggc gtaccgttca gcagcacgct ttttaatcca
     3661 tccaccggac cgtcaaccgg cccttcgctg atggcatcaa tcacactcag caactgcgtg
     3721 gatttcaggt tgtccttcgc ttcgcgcggg gtatgcccct tactgctgcc tttacccatt
     3781 cctcacgctc cataaacgac aaaaccgccc gcaggcggtt tcacatgaaa cattttgcat
     3841 cagcgaccaa tcaccacaac ctgaccacca tccccttcgt ctgccgtgct gatctcctga
     3901 gataccaccc gcgaccccac gcgcatttca ccgtacagaa caggcagaac attgccctgg
     3961 gcaaccatgt tatccagtga ggagaaacag gtgttctgtt tgccgttatc cgttgtctgt
     4021 gtacggggag ttctggcttt cggtgccagc atctgtgcaa caccgccaag cgtcatactg
     4081 gcaccgagag aaaacagcag attactcgcc ataattccta ccccggcatc catatagcaa
     4141 ccgccataac agctgccccc agcaccgcct gaaacacacc gccactttta gctcccacca
     4201 gacgcggtac tatgtggatc actgcaccat ttgccagcgg ctcattaaga cgggcagaca
     4261 attcggtttc acctgcatca cgcccggcaa tgcgcacctg ataccagccg tcactcagtt
     4321 tctgacgaaa cgccgggagc tgtgtggcca gtgcgcggat ggcttcagcc cccgttttca
     4381 cacgaaggtc gatgcggcgg ccaaatcgtt gcaaatcccc gtaaaggcag atgcgtgcca
     4441 ttcccggtga cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtat acctctctcg
     4501 tttactcagt tgttcaggaa tatggtgcag cagctcacca tcgccgcagt aaatggcggc
     4561 atgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggtt gtgctgatga
     4621 caacggcacc tgatacagcc ctgtggcctc cagattatcc agatagagat tctgaccgtg
     4681 acgccaccag tcatccccgc ggtgaaaatc cggcatctca atccccgcca gatgataagc
     4741 atcccggaac agcgtgtaac agtccgtcac cccgtgctca aagcgccgcc cggtgagatg
     4801 cggcacacag cggaacttgt gaatcgcccc ccggcagacc agccaccacg gcaaatcact
     4861 ctgcacctgc agccgccggt cagcctcact cagccagggc agaccaccgg gatgactgtg
     4921 gaccagtgcc acaatctcac cctgcatttg tgcccgcagc cagtcctccg gagccatccg
     4981 gaaccgttcc tccggctcac cggagatatt ctcgctggga agatacctgt ctccctccgg
     5041 cgttctcacc acgaatccac acgactccgc tggcgcacat cgccgggcgt gcgccagaat
     5101 cgctgattct gtctgtgtca tggatttact gagaaagttt attgatggaa aggaaaccgc
     5161 cgaaattacc ggtattgttg cgcaactcac acccgcgcat acacttgctg catttgtcct
     5221 tccggatatc cgtcgttggt ttgtcgaact cgtcagccac tgccctgccc gtgtaaccac
     5281 actcatcaga acggtaagtc cacatacagg tattcgccag catgatacgc cccgggaaaa
     5341 ccgcgccatc cgtctccgtt ggtgtggcca gcacaaaaga ggcactgacc gcgctcagtt
     5401 cgctgcactg ctcgatgcgc cagcggctga tcacctcctg ttccggatcg gcgtcgctgt
     5461 ttccgttgac gaagttcacc gcatccagaa aacgggcgta aaccttacgc cggaccaccg
     5521 ttccgccgac cagactctgc agatcttccg ccatcccggt gaccatgccg tgcaggttag
     5581 agaccgtcag tgtcggacgg gcagcacagc ccctgccgtt cagttcaaac cccgccccct
     5641 gaatggggta tgcctgatac tgccgcccct gccaggtgac cggctcacct ttttcgttct
     5701 gctcattaca gaaaaaataa cgctcaccac cgacctctgt cagatcgatt tcccagagct
     5761 ccacccgggc tgactgctcc gcacgggtgc attcattcag tgtttcctgc cggatatcct
     5821 gcatcagttc accacctgtt caaaatcggc cgttacagtc acccataacg cccccacgct
     5881 tgccgaccat ttacgacaaa cctccctgat cagtttccag tcataaggtg gcgtccactg
     5941 aaatgcgcgg acgccaccgt gccgttccag aaaggctttt aaagatgggt gttcaccttt
     6001 acgaacacgt atcgtcacgc tgtaagtcga caactggtta ttcagtcccg ccgcacgacg
     6061 ctgttcataa ccatcgccca gcttcactgt caccactttc ggctctgata ccacattcat
     6121 atccgggcgc actttccagt gaaacgtctc cattaccggt atgctccact taaccggcca
     6181 ccatcacggg cctgctgttg cataaagtcc gcggccgctt ttttcccgag gtcataaacc
     6241 accttcaggg cagccggacc tatctgcccg ttcgtgccgt cgttattgat ctcaatgttg
     6301 tactgcgggg caaacatcac catgcctgaa ccaccagtat ccgccacaac cccccagctt
     6361 accatcagca ccgcgacgca gtggcagaat ggcttcaggt cccgcttccc ccatcacacc
     6421 cgcgcctttt gcaaaagcaa aaaacgtcgg acggttaacc accgtgccac tgtagcgact
     6481 caaatcagca gactggtaaa caccgccatc agcattagtc gtgaagccga ataaagaacc
     6541 gacgcccttt accgcctgca tcatggccat ctgtgccata attctggcca tatctgacaa
     6601 cagggaagag gtgaaggatt taaaattcag cttaccggtg gtacagaatg tcgccagtgc
     6661 attaccggca ctgctgaatg ccactgtaaa catctgttct gccgttcccg ccgcattatc
     6721 cgcatccgcc gtaaaattct gaaacgcccg catggcaccg tttttccagt cgccctgcgc
     6781 cacttccagt tcctgccagt agcgtttgtt ttcattcagt tgtcgcttca ggctcccc
//
LOCUS       DADLXX010000166         6821 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLXX010000166 DADLXX010000000
VERSION     DADLXX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.169"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            239..2671
                     /locus_tag="JNX27_003660"
     CDS             239..2671
                     /locus_tag="JNX27_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7884702.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2702..4207
                     /locus_tag="JNX27_003661"
     CDS             2702..4207
                     /locus_tag="JNX27_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY7884703.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGNAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4207..5868
                     /locus_tag="JNX27_003662"
     CDS             4207..5868
                     /locus_tag="JNX27_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY7884704.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JNX27_003663"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JNX27_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY7884705.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..6797)
                     /locus_tag="JNX27_003664"
     CDS             complement(6477..6797)
                     /locus_tag="JNX27_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884706.1"
                     /translation="MLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRL
                     RLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQME
                     SALV"
BASE COUNT         2013 a         1618 c         1559 g         1631 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcctatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcaatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga ataacactga ggtagcctga gtttaacgga c
//
LOCUS       DADLXX010000167         6813 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLXX010000167 DADLXX010000000
VERSION     DADLXX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.170"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..731
                     /locus_tag="JNX27_003665"
     CDS             <1..731
                     /locus_tag="JNX27_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7884707.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1034..1804)
                     /locus_tag="JNX27_003666"
     CDS             complement(1034..1804)
                     /locus_tag="JNX27_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884708.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="JNX27_003667"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="JNX27_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="JNX27_003668"
     CDS             complement(2295..2516)
                     /locus_tag="JNX27_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884709.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="JNX27_003669"
     CDS             complement(2517..3200)
                     /locus_tag="JNX27_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7884710.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="JNX27_003670"
     CDS             complement(3264..3575)
                     /locus_tag="JNX27_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884711.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3572..4543)
                     /locus_tag="JNX27_003671"
     CDS             complement(3572..4543)
                     /locus_tag="JNX27_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY7884712.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="JNX27_003672"
     CDS             4914..5873
                     /locus_tag="JNX27_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7884713.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="JNX27_003673"
     CDS             5873..6268
                     /locus_tag="JNX27_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7884714.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..>6813
                     /locus_tag="JNX27_003674"
     CDS             6451..>6813
                     /locus_tag="JNX27_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY7884715.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAE"
BASE COUNT         1861 a         1758 c         1662 g         1532 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctgggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgctcac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcacag gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta gcggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccgcaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc gtccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acacagatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccagcgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaagt agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgctt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 tataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gacccccgac tgttaacgct
     6061 aatcagcggt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaataacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaa
//
LOCUS       DADLXX010000168         6783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLXX010000168 DADLXX010000000
VERSION     DADLXX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.171"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1282)
                     /locus_tag="JNX27_003675"
     CDS             complement(<1..1282)
                     /locus_tag="JNX27_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY7884716.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQ"
     gene            complement(1567..1968)
                     /gene="pemK"
                     /locus_tag="JNX27_003676"
     CDS             complement(1567..1968)
                     /gene="pemK"
                     /locus_tag="JNX27_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HAY7884717.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(1901..2158)
                     /gene="pemI"
                     /locus_tag="JNX27_003677"
     CDS             complement(1901..2158)
                     /gene="pemI"
                     /locus_tag="JNX27_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HAY7884718.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2251..2904)
                     /locus_tag="JNX27_003678"
     CDS             complement(2251..2904)
                     /locus_tag="JNX27_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAY7884719.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(3002..3142)
                     /locus_tag="JNX27_003679"
     CDS             complement(3002..3142)
                     /locus_tag="JNX27_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884720.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            complement(3842..4699)
                     /gene="repA"
                     /locus_tag="JNX27_003680"
     CDS             complement(3842..4699)
                     /gene="repA"
                     /locus_tag="JNX27_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7884721.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(4692..4766)
                     /locus_tag="JNX27_003681"
     CDS             complement(4692..4766)
                     /locus_tag="JNX27_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7884722.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(5011..5259)
                     /locus_tag="JNX27_003682"
     CDS             complement(5011..5259)
                     /locus_tag="JNX27_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7884723.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(5544..5693)
                     /locus_tag="JNX27_003683"
     CDS             complement(5544..5693)
                     /locus_tag="JNX27_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7884724.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(5940..6413)
                     /locus_tag="JNX27_003684"
     CDS             complement(5940..6413)
                     /locus_tag="JNX27_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884725.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            complement(6568..>6783)
                     /locus_tag="JNX27_003685"
     CDS             complement(6568..>6783)
                     /locus_tag="JNX27_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7884726.1"
                     /translation="RRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSH
                     DARKLLQMTGEWLNTTGADQQSPEHRS"
BASE COUNT         1759 a         1667 c         1500 g         1857 t
ORIGIN      
        1 cctgtttaaa atcataattt ggttgttctg ttcttgagtt attaagtaga gttcttatca
       61 gtgtttgcca agcatgattg ataaatttat tttctgattc tttaaaatat cgtctgatta
      121 aagcacataa atcttcgaca cttttctgac gagcgctagc tgcccaacgg cccccttcgg
      181 tttgcaccat tgaacgttgt acactgtctc ctaactgagc gataaatttc tgttcatcgt
      241 caagttgcat gttttcattt atgattaatt catacaaagc aagaaaaaca gcttgatagt
      301 aacgaggaca ttcttgagat gaagatgcat tgcccagaat gtgtgatcta aagttcaaat
      361 tatttgcatc aaataatttt tgaatcatct cataaacgaa gaggtaatta ttgacaattt
      421 tatctgcacc atttgtctga atgtaattct ctaattgagt tcgtgtatgg attgcatgac
      481 tcgtcgaacc ttcaccgtaa aatgtatcga gcgatgttga tcctgatgtt ggcttatcat
      541 ctaaaaatat atagcccaag atatttgcaa ctaactcttc atcgtcagag tctctaatgc
      601 taggcctact aatgatatga tttctaatat agaaatgatt gtctatattt attccataat
      661 cgagtccatc attgcaaatt gatatgttat gtattttatt cagtcccatt atatcgctat
      721 gtgtagtatc tcctcttata attgtcgata ttttccttac taactctgaa aaattactaa
      781 cgcaccccgc acttcgtagt tcttgttttg ataaaatttt accattggaa tttatgcgtc
      841 gaaacacttc gtcaatgtgg tcctctgttg aagagtattc agatatagca aatctatagc
      901 gagcaattaa tgtagaactt tcgcgggata gcactggctc tttttgttga agtttaccac
      961 tgtcttttag ttgttttgtt aatgcaatgg catctaaatt aaagtattta ccatttaatg
     1021 aaaatcgctg ctcaataaaa ctcattaaag cttctagtct ttgcattcca tcaaggattt
     1081 ctcttttgtt gtcaatggaa acaaacaata gcaaagggat agggtattgc tgagtcattg
     1141 atgaaatcag tgctgttttc tcttcgaggg accatactaa ttttctttgg tatcttcgat
     1201 ttataattaa tttatcatcc agataccact cgtataattc ttctgctgtc ttggagtcaa
     1261 tatctaagtt ttcattagcc attagatgta tctcgagtat ttagttaaga aactgatagg
     1321 tcagtatttg catcaatgtt cattgaattt gtatccgaag tcaatctgat gtaggaactt
     1381 caaaactgcg caaaacacgc aaatacgcac aagaaagtag ggctgcgctt ggaattcgga
     1441 aaataaacca catcaaaact actttttagc caaataacgt ttgggaatca ccagaatggt
     1501 gggacaacag cggttttagt gccctaaatc gtacgttttc atccagttgc ccctcaaacc
     1561 ccatgttcaa gtcagaatag tggacaggcg gccaagaact tcgttcatga tagtctccgg
     1621 aacccgttcg agtcgttttc cgccccgtgc tttcatatca attgtccggg gttgatcgca
     1681 acgtacaaca cctgtggtac gtatgccaac accatccaac gacaccgcaa agccggcagt
     1741 gcgggcaaaa ttgcctccgc tggttacggg cacaacaaca ggcaggcggg tcacgcgatt
     1801 aaaggccgcc ggtgtgacaa tcagcaccgg ccgcgttccc tgctgctcat gacctgcggt
     1861 aggatcaagc gagacaagcc agatttcccc tctttccatg tcagatttcc tcctgaccag
     1921 tcgccggtgc atccagccat tctcgttctt cagctgatat ttcagcattc ggatcacact
     1981 gtgccagtag ctcagccagt gaatattgcg ggcgtctgta cggctcaaca atcagccggc
     2041 cattatcaat gaccatgcca acttcattat ctgtgcccag agacagcgca ttcagcagtg
     2101 ccggtgggac ggtcagcata actgagccgc caaccctctt cagtcgggtg gtatgcattc
     2161 ttcacctcca taaaagttat atttaaatat aacatccact aaaaaaacac accaggcttt
     2221 aacgcacaat gtttaataaa aatataactt tcaaccaaac agtaaaccca gcgtggttgc
     2281 ttccatatgc agcaataccg gcagcagcag gccaccactt ctgatcctgg ccactgatgt
     2341 aatcaacccc accaggaaca gttctgccag tgtcagcagg ttctgatact ggctgtgcgc
     2401 ggcgacgaac aacaacgacg ttatcagcgc ccccagccac atcgtccagc agtaccgtga
     2461 acggaagacg ttcagcataa tcccccggaa cagcgtttcc tcattcaacg gggcaaggat
     2521 aaagatggtc agcaacgtca ggatcacgtc aggtatggac ttatcggcaa aaagtttcgt
     2581 cataaatggc tcagcaggca gagccagcgc cttaccgagc agaaatacac cgacatacac
     2641 cacggccatc gcaccgacca gccacggtac gccaacgttg cgcagctgac caacgaccgg
     2701 tagcggagct atccaacggc ggtataccag gaaaacacac agcaggtaca tcagaacagt
     2761 accatgactg aagaacaaat agttttttcc tgatccataa agcagaacgg cctgctccat
     2821 gacaaatctg gctccccaac taatgcccca ggcagccagc ataaccagca taaactgcag
     2881 atattgatta cgtgtttgaa tcattgcatc gcctgtaaat ttttaacttg tcctattttt
     2941 gtcattacca cgtatataca catgtataac aattcagata tcgttaccag gatatgccgc
     3001 atcagcggca tggaaggcgg cactctgttg tttcatatga tacaggagta aaaccgccga
     3061 agcccggcgt aagccggtac tgattgatag atttcacctt acccatcccc agccctgcca
     3121 gaccataccc gctttcagcc atgagagagc ttctgtgcgc ggtcggagtg gtcccgacga
     3181 gggtttaccc gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc cgcttacgag
     3241 cgtgtactga gaacttccag cgagaagact gacagcgatg aagatgtagt tacaacattc
     3301 ataattaaaa gcgactctgt tccggccctt cgggccgggg cggggccgct tttcagttat
     3361 gaggaggggc tttgtggttt cagttctgcg ctggactggg gttttcccgg tgcttgtatt
     3421 tgagtgttgt aactaaaatg gctccggtcg gggcccgccg cttgcggtgg gaggtgcata
     3481 tctgtctgtc cacaggacag gcagtgaata ggttttcttt ttaaatgaat gtaattaagt
     3541 agtttaaagg agatataaac aggtgtttaa aagatacatt gcaccctgta agactggcgg
     3601 ctggcgcttt atgacatgaa cggttgtaac cttatgggga agtcccttgc agtttaatgt
     3661 ggataagcaa aattccccgt ctgtgaggcg tgttttgtat cgaaaacagg gggggccgga
     3721 tgcaccagaa gctggatgat gaggttgttt tttgtatgtg gtgctgtttt tttgtgcatc
     3781 gacgggcttc aggcgtgcga atgcctccgg cgcgggccgg attattcaga ggaggtcact
     3841 ttcaggggga agctgtggcc agccggctgt aattacggtt acgtgacaga atcatgcgct
     3901 ccttcacacg acgctcaact tcgcgtttta ccgcctcacg attggcagtg aagcgccctt
     3961 ccgcgatttc gcgcgtcagc tgccgtttca ccagggtgac aatatcctga cgttccctgt
     4021 ccgcatcacg acgcgcacgg gcacgcttta ttccccggga cttaagctct gtctgataac
     4081 tgcgaaaacg ctcacgaaca aaacgccagg ctttcgctat cagttcatcc atgcccaggg
     4141 tatccagccc ctgctttttg cgttgtttgt tttcccatac cacacggctg cggcgcgcgg
     4201 cggccgctgc ctcctctgat acatcgaggg cagcaaacag tgcagatgtg aacgtgatat
     4261 cggtcggaat gtagcacccg ataagcgggt catattccgt ctggtaggta atcagtccca
     4321 gctctgacag gaacgtcagg gcccgggtgg cacgggtgat ggagagtttt ccggcagcag
     4381 actccgtcgc cagtccgcac tcaatggcca gcgtggtgat ggagcactgg acgcggttgg
     4441 ccagcgggtc atagtggaaa cacagcccct gcaggagcgc atcaatagcc cgtcgacgca
     4501 gcaccggtgg catgcgtcga cgcagaccac gcgaacgggc atgcgccaca tgaatggcga
     4561 aatcaaaacg ggaagtgaag cccaccgcct tttccatcag tttttcgcag aacttcagcg
     4621 ttccggcacc tttacggggt gtaaacaccg gattcgggtt ctttacctgg cggtaatacg
     4681 tttgttgaag atcagtcaca ccatcctgca cttacaatgc gcagaaggag tgagcacaga
     4741 aagaagtctt gaacttttcc gggcatataa ctatactccc cgcatagctg aattgttggc
     4801 tatacggttt ttagatggcc ccggtaatct tctcagtcgc caaacttgtc gaagattatc
     4861 ggggttttca cttttctggc tcctgtaaat ccacatcaga accagttccc tgccacctta
     4921 cggcgtggcc agccacaaaa ttccttaaac gatcagcaat ctatcacgca caccagaaac
     4981 aggcaagaat gtgatgtcgc tacattttag ttagctaaaa gcactcttct gctttatcag
     5041 ttcggcaatc atctcagcct gggtaacgcc ctcagcttca cacatcacct gcaattgcgc
     5101 ttttaacgca gcaggaatga acactctgag ttctttatgt gtagctcgtt ttcttgccag
     5161 ggatgcctgc tgacgttctg caagcgtcag aggattacct tttctgtatg ctcgctttgt
     5221 acctgatgag gaagtcactg cattttctgt ctgcgacatc tcgcctcctc aatactcaaa
     5281 cagggatcgt ttcgcagagg atactacagt tttttgaaat cagcgacttg agaattgtga
     5341 cgaagatctg tgttggcgca caaatcaacg gggattactg ttgtttaatg tgatttttgt
     5401 tgcgaaatgg ctgaaaaaac aggaaatgcc ttaacccggg agccggcccc gggcgggacc
     5461 ggctgtttct ggcatgtcgg gtgtctgccc ctgaaatacc tcacacttcc ggaaagcggg
     5521 gacttccgtc cccgcccggc agtttacttc cgtgcttcgt aggccagagt tacctgcacc
     5581 actgtatttc ccctgtgaat attcagctca cataaccgtt ccctgaatat cagtgaaaaa
     5641 cacaacaccg tggcgcacac ggcgagcaac ccgataaggg catacttcgt cataaacttt
     5701 gacctctctg caaggaagag gctacaatca gtagtgcaat gtacttcatg cagacctcac
     5761 gagttaatgg attaacaagt ggggtctttg catttctggt acctgcgtct tgcctgaacc
     5821 agaaaggctc agacacccgc cgcgataata ccacattccc tgttgctgac tattacctgc
     5881 attgcccgct ttttactaca gaagcacgaa ggcgtcactc cagccaataa gtatgctttt
     5941 tatttacatc catttttaat ccggtaccga cgttttcctc cagttaactc gtttttcgta
     6001 tgactcatgg caataaaatt ttctttacca tatgacaaca tcccaataat atcaaagttg
     6061 tcttttaatt tgatggcatc cttttcactt acagcgacaa taagttgttt accaaatttt
     6121 ctgaccatca ttgcaaactg tgatactgct gatccaccac ctaaatcagg ccacccagca
     6181 caattaaaaa tgattgtctg ctcttcacta ttcattgcat ccctgataaa ggagacagca
     6241 tcctcatttc tctctacatc tgaatcactg ttaatctgag tgaaatcata atatttagaa
     6301 cctggtatag aatacccctt aaaaaactta tcaatgtggg aggcagagtt cccaacaata
     6361 agaatattgt gttcacgctt aatcatcaat tctgatacat catcatattt catatattca
     6421 ccattattat ataaagtttg ggtacaggtg gttccggaat ttactctgtg accaccattt
     6481 cccgcttttt attacagaaa cgccgggagc ggggaaaact tgctttttcc cgtttccggg
     6541 gttggacaac tgagcaacgc gaaggcgtca gctacgatgt tccggggact gctgatcagc
     6601 ccctgttgta ttcagccatt ctccggtcat ctgcagcagt tttctggcat cgtggcttct
     6661 gagcaacgga ataccagcct gcctcagaac ctcttccaga agaatatccc ggcgccggcg
     6721 ttccggtcgt aaatggctgg catcatccag ctctactgct gcaacaatac tgaacgaacg
     6781 ccg
//
LOCUS       DADLXX010000169         6781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLXX010000169 DADLXX010000000
VERSION     DADLXX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.172"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..3524
                     /locus_tag="JNX27_003686"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JNX27_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6749
                     /locus_tag="JNX27_003687"
     CDS             3534..6749
                     /locus_tag="JNX27_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY7884727.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSPEWQSAITQAINLIGEHKPSVPARTMAALAFIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAVVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPSYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSQMPEILVSPPWRGRKKKTSIRHFSLPD
                     LTLAPQTYRSPNKHWTPEQQTAIDHFSAMSFDERLATRGADIFLRELGFDVHSLEFNK
                     RLLNGQLDAFDAQQLQEYTSIGLLRTYHAHQAKSKQYREDATNALLAQDSIALMGGWR
                     TFQHGFYYGNEQRWGIWNLYLIAQMPREMAVSCWQQIVEADFHYTGVEYLLSVLGTDA
                     LPGLNTAFARHPKEIFPLLIHFGATELALPIARVWHLFAGQRNLARQWILQWPEHTAT
                     ALIPLVFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPI
                     EIYPARIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQL
                     KTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTHILAWPQE
                     GKSARAVSGLNILTLMNNDMALMQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEE
                     LADRLVPTLGLNDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQ
                     LKAPEALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQ
                     RLIWGVIRQMNRVVYSTPFVWPQRGSSAMRKMSQLTCLRTL"
BASE COUNT         1797 a         1781 c         1634 g         1569 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADLXX010000170         6780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLXX010000170 DADLXX010000000
VERSION     DADLXX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.173"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            337..579
                     /locus_tag="JNX27_003688"
     CDS             337..579
                     /locus_tag="JNX27_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884728.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            693..1331
                     /locus_tag="JNX27_003689"
     CDS             693..1331
                     /locus_tag="JNX27_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7884729.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1328..2065
                     /locus_tag="JNX27_003690"
     CDS             1328..2065
                     /locus_tag="JNX27_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7884730.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPFTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            2065..4161
                     /locus_tag="JNX27_003691"
     CDS             2065..4161
                     /locus_tag="JNX27_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7884731.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ERMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4208..4486)
                     /locus_tag="JNX27_003692"
     CDS             complement(4208..4486)
                     /locus_tag="JNX27_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884732.1"
                     /translation="MKRNLIKVVKMKPYFATLMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4487..4630
                     /locus_tag="JNX27_003693"
     CDS             4487..4630
                     /locus_tag="JNX27_003693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884733.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4756..5166
                     /gene="psiE"
                     /locus_tag="JNX27_003694"
     CDS             4756..5166
                     /gene="psiE"
                     /locus_tag="JNX27_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY7884734.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNX27_003695"
                     /pseudo
     CDS             complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNX27_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1698 a         1696 c         1785 g         1601 t
ORIGIN      
        1 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
       61 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
      121 tacgcagatt aattactttt gtttttattt taagtttatg atttttattg ttatttaaat
      181 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
      241 ctcgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
      301 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
      361 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
      421 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
      481 accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg gtggcggtga tggttctaat
      541 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
      601 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
      661 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
      721 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
      781 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
      841 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
      901 aagacggaca acaaaaatgg gtcactcagg atcaatccac tcttgtcaca caacatggtc
      961 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     1021 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     1081 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     1141 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     1201 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     1261 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     1321 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     1381 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     1441 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     1501 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     1561 gctgacacgg ctggcagagc agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     1621 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     1681 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     1741 gtgggtcgga ccaccgccgt tcacggtcac gttgttcggg cttatcagcc atcctggcaa
     1801 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     1861 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     1921 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     1981 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     2041 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     2101 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     2161 gatttcggtg gcgtaggatt attacaaact cccaccgagc gtatggcacg ggaaggggag
     2221 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     2281 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     2341 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     2401 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     2461 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     2521 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     2581 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     2641 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     2701 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     2761 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     2821 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     2881 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     2941 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     3001 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     3061 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     3121 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     3181 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     3241 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     3301 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     3361 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     3421 gatttgtggc tgacggacca tctactgacc actggcagcc tgtttgcaaa tcttgccaac
     3481 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     3541 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     3601 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     3661 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     3721 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     3781 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     3841 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     3901 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     3961 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     4021 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     4081 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     4141 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     4201 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     4261 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     4321 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     4381 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagtag
     4441 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     4501 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     4561 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     4621 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     4681 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     4741 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     4801 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     4861 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     4921 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     4981 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     5041 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     5101 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     5161 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     5221 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     5281 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     5341 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     5401 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     5461 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     5521 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     5581 ccgagcctga gtgtaaaacg cggtactgag gctaaacata ccgattgcca ttccgagtgc
     5641 gccgataatt tgcagtggct tacgaccaaa tttatccaca gtcataattg ccagtacggt
     5701 aaaggtgagg ttgataactc cgacaataat ggtctgcaac agcgcgatat ccgtgctggc
     5761 ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc acattgatgc cgacaaattg
     5821 ctggaagatg gagagcataa cgccgattac aatcacgccc acgccaaaca tcagcagacg
     5881 accaccggtt ttgcggccat gatccaggga gtgtttaatt tcctgtactg cctgagttgc
     5941 aagcgtgttg cccataattt tgcgcaggat actttccgtc tgttcttgct tgccgcgtga
     6001 catcagccag cgaggacttt ctggcacggt atacagcagc attaagaaca gcagtgcagg
     6061 gatacattcc gaggcaaaca tataacgcct gccgtcagta ttcagccagc tggcatcacc
     6121 ggaacgggca ataaaatagt ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa
     6181 ctggttaaat gaaaccagtt ttccgcgaat atgagctgga gccagttccg caatatacat
     6241 tggtgaaagc attgaggcta aaccaacgcc aataccgcca ataatacgat aaataacaaa
     6301 ttccgggaca taacctgcca gataaatagg tactgtgttg tccgggttta tagaggtaaa
     6361 accaagttct ggccaggcag aacctacacc agaaataaaa aacaggacag cagaaatctt
     6421 aagtgaatca cgacggccga agcggttact gcaataacca ccgagggcac cgccgatgat
     6481 gcaaccaatc agagcgctgg ctacgcaaaa tcctaacagg gagttggcag cggattcact
     6541 taagttttgt ggagcaacaa agacggtatt gagtgactca acagtaccgg aaataacggc
     6601 ggtgtcgtag ccaaataata aaccacctaa tgtagcgact aaggtaatcg aaaatatata
     6661 actggaacta tactgggtat tcattcagac ctgccttaga ccattctgat cttttatcag
     6721 tgcattgatg ttggaattgt caggcagtta aggcaattat cattttttac aatgcgttta
//
LOCUS       DADLXX010000171         6678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLXX010000171 DADLXX010000000
VERSION     DADLXX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.174"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..176)
                     /locus_tag="JNX27_003696"
     CDS             complement(<1..176)
                     /locus_tag="JNX27_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7884735.1"
                     /translation="MEYRTWITEALRLHFEEYLPRVVAGRRLGVPKSTVCGMFVHFRN
                     AGLSWPLPAGMSEQ"
     gene            339..524
                     /locus_tag="JNX27_003697"
     CDS             339..524
                     /locus_tag="JNX27_003697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884736.1"
                     /translation="MDARNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            572..1001
                     /locus_tag="JNX27_003698"
                     /pseudo
     CDS             572..1001
                     /locus_tag="JNX27_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            1188..1352
                     /locus_tag="JNX27_003699"
                     /pseudo
     CDS             1188..1352
                     /locus_tag="JNX27_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1846..2424
                     /locus_tag="JNX27_003700"
     CDS             1846..2424
                     /locus_tag="JNX27_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884737.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2533..2791)
                     /locus_tag="JNX27_003701"
                     /pseudo
     CDS             complement(2533..2791)
                     /locus_tag="JNX27_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2775..3331
                     /locus_tag="JNX27_003702"
                     /pseudo
     CDS             2775..3331
                     /locus_tag="JNX27_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3355..3761
                     /locus_tag="JNX27_003703"
                     /pseudo
     CDS             3355..3761
                     /locus_tag="JNX27_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4345..5061
                     /locus_tag="JNX27_003704"
     CDS             4345..5061
                     /locus_tag="JNX27_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884738.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            5613..6017
                     /locus_tag="JNX27_003705"
     CDS             5613..6017
                     /locus_tag="JNX27_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884739.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAIPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            6080..6379
                     /locus_tag="JNX27_003706"
     CDS             6080..6379
                     /locus_tag="JNX27_003706"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7884740.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQKAATYFAKRLK"
     gene            6376..>6678
                     /locus_tag="JNX27_003707"
     CDS             6376..>6678
                     /locus_tag="JNX27_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7884741.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASR"
BASE COUNT         1929 a         1327 c         1659 g         1763 t
ORIGIN      
        1 tcctgctccg acatacctgc gggcagaggc catgaaaggc cagcgttgcg gaagtgcacg
       61 aacataccac agacagttga ttttggtacg cccaggcgac gtccggctac aacccgtggt
      121 aaatattctt cgaaatgaag gcgtaaagct tcagtgatcc aggtccggta ttccatacga
      181 tagtgtccac ttcaaaatga tggacattat ttttgtggag ccggaggaaa cagaccagac
      241 ggtttaaatg agccggttac gaagaagctg aggttctgcg ctttgataat tttgacgatg
      301 ccgttgagtg ttgcgcagac ctgaatattc cgttctttgt ggatgccaga aacaaaaaac
      361 tggtcttctg gtttgtacgt gttgatgacg aagggtatcc ggaaattgcc cgctgcatgg
      421 agcgggaatt tgcgacaatt cctgccggta tcaacgccga cggcatgtat tgccctgaat
      481 gcggtacggt tcactggccg gatggcgtta ttccgccttt ctgatgcttc cccgttttgc
      541 cgatattttt cagcagggaa accgatggct taactggctg gagaagcagc cggtgcagat
      601 gagccgcctg gaacattatg ccggtcagga tgaaattggc ctgcgttaca acagccaccg
      661 gacaaaacga gaagaaaacc ttgtgatgag tggcgacgag tttatggaaa ggttctcctg
      721 gcatgtggcg gataaggggt tccggatggt gatacggggt cctgagtccg gtgaagcggc
      781 gattactgga agatgtggtg tacgccataa cggagacagt gagaaaaacg gcgaggcaaa
      841 tcacaaggag agggatgtat cagcagtgac tgaaggttga cccgctgaag tgcgagctgc
      901 gcggaggtca gatgcggttt acggggctga agaggggtta ccgtctggca gagcaggtta
      961 tgatgcatga gccgctggct cgaatacggt ggtgcggctg agagtcgcat aggggaagtt
     1021 gcgtccatgt tgaggggaac ggagcaaaaa accatcagag atacattgaa tcaatcaatg
     1081 taagcaatat taagtgtcta taccgattac ttgatgatca ggttgtgttg aataaacgcg
     1141 cattcttcgg tgctttttta atcttttggg gatttaaatt cctatcgatg aactgtcgat
     1201 atgaaaaagc gagcatcata ctgacgtcaa acaaaggggt cgcagactgg ggagaaatgt
     1261 tcggagatca cgtgctggca acagcgatcc tagatcggct gctacacgct gaatatcaaa
     1321 ggagagagtt accggttaaa agagaaacgt aaagctggag tgctgaccaa aaacacaacg
     1381 ccaatcagtg atgatgaaat ggtggaaaac ggacagcatc agtaacgaaa gtatcttagc
     1441 aggcatgaaa atcgtccgca tcccctaaac ggtcaaaatg cttctgccgt gaaggaggga
     1501 gaatgccatc tcatcactta tatctggcga atgaaacaca gatggtcggc cttgccccca
     1561 aacacacggc ggtgcaaaat ggaatggcag aaaacttcgt caaaacgatg aaatatgatt
     1621 aaatcagtgt tatgttcgaa cctgacgtgt taacagcagt aaagtagtct cgcgaaggtg
     1681 ttcaaacatt ataatgaatg gcatcctcat agtgcgcagg gttatcgctt gctacggaaa
     1741 tatctgaagc gacgtaccag taatggggaa aatgatagct ggtttctgaa aatatagagg
     1801 aaaatgtgat atatatatcc tgttaatatt taaggagaat ataaaatgaa agtctcgttt
     1861 aagtctttag gctatatttt tcatgatata tataataaaa aacacactat cgatgagttc
     1921 aacgatgtgg taagaaaagc agttttatca ggtaaaatta acgagctgaa tgcatgtcat
     1981 aaagtggcaa tttttcttgc cgaaaaagac aatgagataa caaagaaaga caaagccaag
     2041 ataatagata cattaactga aaactattcc attgaatttc aacaattaat gaatattagt
     2101 gagcgcacgt taaattcatc tttatatatt actcctggtg aaagtggttt tgtcagtttt
     2161 gttaatcgtg aaggaaaaat atgtcatact gcatatgtaa aatcatcaga taatagcatg
     2221 gcatattacc atgcaaatgg tagcagcatt gacaaatata ttacggatat gtgcggcctt
     2281 atttgtatga ggcatattga gtctactggc attatttttt atatgcttga tgaaaaagtt
     2341 ttatctgcca ttgctgaatt tatgaatgaa aaaggatggc gggctgcgtt ttgttcagca
     2401 aagaacttat ataaatgtgt ttaattttag aaaaactatt tggtaaaatc caacaagagt
     2461 cgagaggttc gcgttgcttc agcagggcct cagcctgttc aaccagactc agttgcaggt
     2521 cgtgacgcag atttagtggt ggtggttgat tgtcagatac cagccgatga tcttgtagtg
     2581 catatcatca gactttgaga agcatatggt tttgcgggta agccgcttaa gatgtgtccg
     2641 caggttcaga ttatgtcgct ctatccgctg agtgcatttc ttgcttataa catgactggt
     2701 tgaggacaat aaagttcggt ataccggctc aggcatcggt catgtagaag gcaatgctaa
     2761 actggctcag caaactaaga gcagcgcgca tccacttacc caatgcggtt gagaaaataa
     2821 ccttcgatcg ctttcatgtg gcgaaacaac cgggtgaggt ggtggataaa acccgccaga
     2881 atgaacatcc gcatcttcct gttgaaagtc gtcgtcaggc aaagggaact cgcttcctgt
     2941 ggcagcacag cgataaatgg atgaccgaat ccaggcagga aaagctgata tggctgcgtg
     3001 cgcagatgaa gctgacgagc ctgtgctggg cgctgaaaga actggcaaag gatatctgga
     3061 gcagaccatg gagcgaggaa agacgcaacg actggcagag atggctgcgg ccaacagtga
     3121 cgtccccatg atgaagaatg tggctaaaac gatagggaaa aggttgtacg gtatcctgaa
     3181 cgccatgcgc catggcgtat caaacggaaa tgcggaagca ctgaacagca agatcaggct
     3241 gctgagaata aaagccaagg gctaccacaa ccgggagcgc tttaagctgg gagtgatgtt
     3301 ccactatggg aaactgaata tggcgttcta agccttccca ccatgatcgg ggaagaccca
     3361 gatatgaaca ggcacattca gagttacttt aaagagctta ctgaacgtct gaggtgttag
     3421 agccatgact gccgcagcat tgctggcgga aatgcctgaa tcgagcagcc tctcgagacg
     3481 tgaaatcagc gcacttgtcg gtgttgctca ggtaaaccgg gactcgggaa ctctgcgcgg
     3541 tcgacgaacc attttcggtg attgtgctgg ggaagaacag ctctgtacat ggcggcgctt
     3601 gaggcccccg gtttaatctg gtgataaagg cgttttacat gcgcctactt gcagccggaa
     3661 acgccaaaaa agtggcactg gttgcctgta tgcgtaagtt gctgaccatt atgaacgcaa
     3721 tgctcagaaa aaacgaagaa tggaatgaat cgtatttatg aaaactggat tgtgggccat
     3781 aaatattcca aaaagatagc ccgctccgtt tattaccgga acgggcgaga gcatgacatt
     3841 gtctgttatt ataatgagac tgctgattct acttattttt aatttcaaga aaagcgtttt
     3901 ctacaaagaa ttatgagatt taatgttttg tttagacaat attatcattc tgttcaataa
     3961 tgaaagtctt catcaacaac aagtaaaaac aacatccctc tcatcgaacc ggacttgcag
     4021 atttcccgca tccggctctc ttgcaacaca tcagaccttc acgttcgggc ggttatgggg
     4081 aaggtccagc aagctgtatg agccctaact gtttgaatac cagtagatta ccagaaactt
     4141 ctatcgattg aagacattac caactagcgt tactaaatgt tgaccaagtt ttgttcactt
     4201 gtctggtttt gatagtagca ttctgcagag tattttgata tgataaatat gcattaacaa
     4261 tgatattatt gttaatttcc tagcataaaa attaaaagat caacttttat ctcgaatatt
     4321 ttttacatta tcagttggtt tcttatgaat gacaattcat tgcttagaaa ttcttcatta
     4381 ttcatcgcct atatgggatg tgtagggtgg gtcagtgctt attcttatgg ctggggaacc
     4441 tctttttact atggatttcc atggtgggtt gttggtgcag gtttggatga tgttgccaga
     4501 agtttactct atgctatcat agtaatgggt atacttttta ccggttgggg gattggtgtt
     4561 ttattttttc ttcttataaa gaaaagaagt aaaatacaag acttgagttt ttttagacta
     4621 ttttttgcta ttaccctact gtttttccct gtaatattcg aacttttaat tctcaaacaa
     4681 tatttcatac ttccattatc actttcatgc attattagtt cattggttat ttcaataata
     4741 attcgtatat atggacggat cttttctgta tcatgttttt cagatatccc ttttgttaga
     4801 aaacaccgca ttaagttaat tatggctggt tttttggttt atttttggct tttttccttt
     4861 cttgttggct ggtataaacc tcaattaaaa aaagaatatc agatgctttg ttataataat
     4921 agttggtatt atgttcttgc tcgttatgat tcacggctcg ttttgtctag ttcatttaaa
     4981 gatgacagta acagattctt aatatttaat acagaacagt cgggatttta tgaaatcaat
     5041 gatgtttatg tcagaaagta ataatcctct cccacaaagt gaattaaatg aatatataat
     5101 gcgttcgaag tatatgtatt gagctgtcat actgagaaac aaaccttagg gcagcacaat
     5161 taaagccaga aacaactatg caagaaagtt actaaataag tgctcggaac agtaaaacac
     5221 tcagggggga ctcagtccgg attatgtaat cagataaatt gccaaacatc acaaatcacc
     5281 agccggactg agtgatgcca agcattgcac caataccccg tgatgaacga tgcctgatgc
     5341 agaaagctcc ctataaaaca aagaacaaaa actacgaccg gcgattcacc gtcatactga
     5401 tgctgcatcg tggtgacact gtctgtcact ttcatgttga ctgaacaact ctgctgcgaa
     5461 gaaggtgtac cgccggcggg atttgattgt ccggcgtcta gcttccactt ccctgatgga
     5521 actgaaagtt gttatggccg gtcagcttgc cctgtccgca ggacatgagg tgctgtgacg
     5581 gacagcacca gtaacggaaa gtgacaatga aaatgaatac tgcgctgata gttgcactaa
     5641 tgtgtatgtg gtatgcggta cccgctgctg caaaagagac tttactggct ataccacgta
     5701 attcaacaga acattgctat gcggagatta atgtgcacgg accatatggg gtgtatttcc
     5761 gagtggtccc gcatcccccc gggggaaaga gctgggtcga atgtaacagt gactactatt
     5821 actctgataa accaccggga gtacagatac tcggaacccg tgcagggtgt cgggtatacg
     5881 gaatctgcgg gaccacctcg acactgcatg tggctggaag aggggttgtg tgtattaaaa
     5941 atatatgcag tccacgtggg atgataattc acaggatacg gaaacgtcct gtagtggctg
     6001 taagcgatga gatgtgatct tacccagcaa tagtggacac gcggctaagt gagtaaactc
     6061 tcagtcagag gtgactcaca tgacaaaaac agtatcaacc agcaaaaaaa cacgtaaaca
     6121 gcattcgcct gaattttgca gtgaagccct gaagcttgct gaacgcatcg gtgttgctgc
     6181 cgcagcccgt gaactcagcc tgtatgaatc tcagctctat gcgtggcgca gcaaactgca
     6241 gcaacagatg acctcgtctg agcgtgaaag tgaactggcc gctgaaaatg cccgcctgaa
     6301 gcggcaactg gcggagcagg cagaggaact ggctatcctc caaaaggccg cgacatactt
     6361 tgcgaagcgc ctgaaatgaa gtatgtcttt attgaaaaac atcaggctga gttcagcatc
     6421 aaggcaatgt gccgcgtgct ccgggtggcc cgcagcggct ggtatacgtg gtgtcagcgg
     6481 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgct
     6541 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     6601 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     6661 agggcaaagg cctcccgg
//
LOCUS       DADLXX010000172         6524 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLXX010000172 DADLXX010000000
VERSION     DADLXX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.175"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1968
                     /gene="acnA"
                     /locus_tag="JNX27_003708"
     CDS             <1..1968
                     /gene="acnA"
                     /locus_tag="JNX27_003708"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY7884742.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAAS
                     NELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTQYLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2032..2622)
                     /gene="ribA"
                     /locus_tag="JNX27_003709"
     CDS             complement(2032..2622)
                     /gene="ribA"
                     /locus_tag="JNX27_003709"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7884743.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2792..3556
                     /gene="pgpB"
                     /locus_tag="JNX27_003710"
     CDS             2792..3556
                     /gene="pgpB"
                     /locus_tag="JNX27_003710"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7884744.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3705..4013
                     /gene="lapA"
                     /locus_tag="JNX27_003711"
     CDS             3705..4013
                     /gene="lapA"
                     /locus_tag="JNX27_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7884745.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4020..5189
                     /gene="lapB"
                     /locus_tag="JNX27_003712"
     CDS             4020..5189
                     /gene="lapB"
                     /locus_tag="JNX27_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7884746.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAVADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5382..6119
                     /gene="pyrF"
                     /locus_tag="JNX27_003713"
     CDS             5382..6119
                     /gene="pyrF"
                     /locus_tag="JNX27_003713"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7884747.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6119..6445
                     /gene="yciH"
                     /locus_tag="JNX27_003714"
     CDS             6119..6445
                     /gene="yciH"
                     /locus_tag="JNX27_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7884748.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         1595 a         1522 c         1771 g         1636 t
ORIGIN      
        1 atgttaggcc agccggtttc catgcttatc ccggacgtag tgggctttaa acttaccgga
       61 aaattacgtg aaggtattac cgccacagac ctggttctca ctgttaccca aatgctgcgc
      121 aaacatggcg tggtggggaa attcgtcgaa ttttatggtg atggtctgga ttcactaccg
      181 ttggcggatc gcgccaccat tgccaatatg tcgccagaat atggtgccac ctgtggcttc
      241 ttcccaatcg atgcagtaac cctcgattac atgcgtttaa gcgggcgcag cgaagatcag
      301 gtcgagttgg tcgaaaaata tgccaaagcg cagggcatgt ggcgtaaccc gggcgatgaa
      361 ccgattttta ccagtatgtt agaactggat atgaatgacg ttgaagcgag cctggcaggg
      421 ccaaaacgcc cacaggatcg cgttgcactg cccgatgtac caaaagcatt tgccgccagt
      481 aacgaactgg aagtgaatgc cacgcataaa gatcgcctgc cagtcgatta tgttatgaac
      541 ggacatcagt atcagttacc tgatggcgct gtggtcattg ctgcgataac ctcgtgcacc
      601 aacacctcta acccaagtgt gctgatggcc gcaggcttgc tggcgaaaaa agccgtaact
      661 cttggcctca agcggcaacc gtgggtcaaa gcgtcgctgg caccgggttc gaaagtcgtt
      721 tctgattatc tggcaaaagc gaaactgaca cagtatctcg acgaactggg gtttaacctt
      781 gtgggatacg gttgtaccac ctgtattggt aactctgggc cgctgcccga tcctatcgaa
      841 acggcaatca aaaaaggcga tttaaccgtc ggtgcggtgc tgtccggcaa ccgtaacttt
      901 gaaggccgta tccatccgct ggttaaaact aactggctgg cctcgccgcc gctggtggtt
      961 gcctatgcgc tggcgggaaa tatgaatatc aacctggctt ctgagcctat cggccatgat
     1021 cgcaaaggcg atccggttta tctgaaagat atctggccat cggcacaaga aattgcccgt
     1081 gcggtagaac aagtctccac agaaatgttc cgcaaagagt acgcagaagt ttttgaaggc
     1141 acagcagagt ggaaggaaat taacgtcaca cgatccgata cctacggttg gcaggaggac
     1201 tcaacctata ttcgcttatc gcctttcttt gatgaaatgc aggcaacacc agcaccagtg
     1261 gaagatattc acggtgcgcg gatcctcgca atgctggggg attcagtcac cactgaccat
     1321 atctctccgg cgggcagtat taagcccgac agcccagcgg gtcgatatct acaaggtcgg
     1381 ggtgttgagc gaaaagactt taactcctac ggttcgcggc gtggtaacca tgaagtgatg
     1441 atgcgcggca ccttcgccaa tattcgcatc cgtaatgaaa tggtgcctgg cgttgaaggg
     1501 gggatgacgc ggcatttacc tgacagcgac gtagtctcta tttatgatgc tgcgatgcgc
     1561 tataagcagg agcaaacgcc gctggcggtg attgccggga aagagtatgg atcaggctca
     1621 agccgtgact gggcggcaaa aggacctcgt ctgcttggaa ttcgtgtggt gattgccgaa
     1681 tcgtttgaac gaattcaccg ttcgaattta attggcatgg gcatcctgcc gctggaattt
     1741 ccgcaaggcg tgacgcgtaa aacgttaggg ctaaccgggg aagagaagat tgatattggc
     1801 gatctgcaaa atctacaacc tggcgcgacg gttccggtga cgcttacgcg cgcggatggt
     1861 agccaggaag tcgtaccctg ccgttgtcgt atcgacaccg cgacggagtt gacctactac
     1921 cagaacgacg gcattttgca ttatgtcatt cgtaatatgt tgaagtaaca agtatttgct
     1981 tgccggttat tttgcttccg gcaagcaaat gaattacaca atgcaagagg gttatttgtt
     2041 cagcaaatgg cccattttct cggctttggt atcgagataa tgttcgttat tggggttacg
     2101 acctacaatc aatggtacgc gttcaacaat attaatccct gcttcggtca gaatttcgac
     2161 ttttttcggg ttattggtta acaagcggac ttcattgacg ccaaggagtt tgaacatatc
     2221 agcgcaaaga gtgaagtcgc gctcatcagc ggcgaagcct aactggtggt tagcctctac
     2281 ggtatcgtaa ccttgatcct gcagtgcgta agcgcggatt ttattcagca gaccaatgtt
     2341 acgaccttcc tgacggtgat acagcaaaat accacggcct tcctcggcaa tttgcgtcaa
     2401 tgccgcttcg agctggaagc cacaatcgca gcgcaagctg aacagggcgt caccggtcag
     2461 acattcggaa tggacgcgcg caagtaccgg ggtatgcccg gaaatatcgc catagactag
     2521 cgcgacatga tcgtgtccgg ttgccagttc ttcaaatccc accatcagga aatcgcccca
     2581 tggggttggc agtttggctt ctgccacacg tttaagctgc atgaaattct ccagataatg
     2641 ctggttctgt attggcttat tttgccataa cgagaagggg ttcacctaat cacgacgcgt
     2701 cgatcgttca cggaatggca caaatctgtc aatttttctt ggaactggca ttttcagtta
     2761 tgattgtggg acttatcaaa aaggagaggc catgcgttcg attgcccgac gtaccgcagt
     2821 gggagctgca ctattgtttg tcatgccagt agccgtatgg atttctggct ggcgttggca
     2881 acctggagaa caaagttggc gactaaaagc ggctttttgg gttactgaaa ctgtcaccca
     2941 gccctggggc gtcattacac atttgatttt gttcggctgg tttctctggt gtctgcgttt
     3001 tcgcattaag gctgcctttg tattattttc cattctggcg gcggcaatcc ttgttggaca
     3061 aggcgttaaa tcctggatca aagacaaagt acaggaacca cgaccttttg ttatctggtt
     3121 ggaaaaaaca catcatattc cggttgatga gttctacact ttaaagcgag cagaacgcgg
     3181 aaatctagta aaagaacagt tggctgaaga gaaaaatatt ccacaatatt tgcgttcaca
     3241 ctggcagaaa gagacggggt ttgcctttcc ttccggtcac acgatgtttg ctgccagttg
     3301 ggcactgctg gccgttggtt tgctgtggcc gcgtcggcga acgttaacca ttgctatctt
     3361 gttggtctgg gcaacgggag tcatgggaag ccgcctgcta ctcgggatgc attggccacg
     3421 cgatctggtg gtagctacgt tgatttcgtg ggcgctggtg gcggtggcaa cgtggcttgc
     3481 gcaacgaatt tgtgggccat taacaccacc tgcggaagaa aatcgcgaaa ttgcacaacg
     3541 agaacaagaa agttaacgct ggttgatttt ccgaatttag gccttaaatc atcaacaatg
     3601 cgcgtggatg ccatttcgca gacggcgcga aaatggtact ttaaagggct attgcggtaa
     3661 gttgaccata atttattcgc tctaaccaca taacgggaag taatgtgaaa tatttactca
     3721 ttttcttact ggtgttagcg atcttcgtga tttcggtcac gttgggtgcg cagaacgatc
     3781 aacaggtgac gtttaattat ctgttagcgc aaggggagta ccgtatttcc acattgctgg
     3841 cggtattgtt tgctgcgggg tttgctatcg gttggttgat ttgtggcctg ttctggctgc
     3901 gagttcgtgt ttccctggcg cgcgctgaac gtaaaataaa gcgactggaa aaccagcttt
     3961 cacccgcgac tgacgtggct gtagtgccgc actcgtcagc ggcgaaggaa taactttcta
     4021 tgctggagtt gttgtttctg cttttgcctg tagccgctgc ctatggctgg tatatgggcc
     4081 gcagaagtgc gcaacaaaac aagcaagatg aagccaaccg cttgtcgcgt gattacgtag
     4141 cgggggttaa cttcctgctt agtaatcaac aggataaagc ggtagacctg tttctcgata
     4201 tgcttaaaga ggatacaggc accgttgaag cccaccttac gctcggaaac ctgttccgtt
     4261 cgcgtggcga agttgatcgc gctattcgca tccatcagac cctaatggaa agcgcctcgc
     4321 tgacctatga acagcgtctg ttggcgattc aacaactggg gcgtgattac atggccgccg
     4381 ggttatacga ccgcgcggaa gacatgttca atcagctgac cgatgaaact gacttccgca
     4441 ttggcgcgct gcaacagttg ctacaaatct accaggctac cagtgagtgg cagaaagcaa
     4501 ttgatgttgc cgaacgcctg gtgaagctgg gtaaagataa acagcgcgtc gaaattgccc
     4561 atttctactg tgagttagcc ctgcagcata tggccagcga cgatctcgat cgtgccatga
     4621 ccttgctaaa aaaaggggcg gtggcagata aaaacagcgc ccgcgtatcc atcatgatgg
     4681 gacgcgtgtt tatggcgaaa ggagaatacg ccaaagccgt cgaaagtttg caacgcgtca
     4741 tatcccagga cagagaactg gtcagcgaaa cgctggaaat gttgcaaacc tgctatcagc
     4801 agttgggtaa aactgccgaa tgggcagaat tcctgcaacg cgcggtggaa gagaacaccg
     4861 gtgccgatgc tgaattgatg cttgctgata tcatcgaagc gcgcgacggt agtgaggctg
     4921 cacaggtcta tattacacgc cagcttcagc gtcatccgac catgcgtgta ttccataagc
     4981 taatggatta ccacttaaat gaagcggaag aagggcgcgc caaagagagt ctgatggtgc
     5041 tgcgtgacat ggttggcgag aaggtgcgta gtaagcctcg ttatagctgc cagaaatgtg
     5101 gttttaccgc atacaccctc tactggcatt gtccgtcttg tcgtgcctgg tcaactatta
     5161 aaccgattcg cggtcttgat ggcctgtaat ttttaaaaaa atccgacttt agttacaaca
     5221 tactagttat tgatgtccca ttgtgctccg gcaatgacgg agcagaaaaa gcctgctagg
     5281 ggaacaatcg caactgttaa ttttttattt ccacgggtag aatgctcgcc gtttacctgt
     5341 ttcgcgccac ttccggtgcc catcatcaag aaggtctggt catgacgtta actgcttcat
     5401 cttcttcccg cgctgttacg aattctcctg tggttgttgc ccttgattat cataattgtg
     5461 atgacgcgct gtcctttgtc gacaagatcg acccacgcga ttgtcgtctg aaggtcggta
     5521 aagagatgtt tacattgttt gggccacagt ttgtgcgcga acttcaacag cgtggttttg
     5581 atatctttct tgacctgaaa ttccacgata tccccaacac tgcagcgcac gctgtcgctg
     5641 ctgcagctga cttaggcgtg tggatggtga atgttcatgc ctccggtggg gcgcgtatga
     5701 tgaccgcagc gcgtgaggca ctggttccgt ttggcaaaga tgcaccgctt ttgattgctg
     5761 tgacagtgtt gaccagcatg gaagccagcg acctggccga tcttggcgtg acactgtcac
     5821 ctgcagatta tgcagaacgt ctggcagcac tgacgcaaaa atgtggcctt gatggtgtgg
     5881 tgtgttctgc tcaggaagct gtgcgcttta aacaggtatt cggtcaggag ttcaaactgg
     5941 ttacaccggg catccgtccg caggggagtg aagctggtga ccagcgccgc attatgacgc
     6001 cagaacaggc gttggcggct ggtgttgatt atatggtgat tggtcgcccg gtaacgcaat
     6061 cggtagatcc agcgcagaca ctgaaagcga tcaacgcctc tttacagcgg agtgcatgat
     6121 gagtgattcc aacagccgtc tggtctactc aacggaaacc gggcgtattg atgagcccaa
     6181 agcggcccca gtacgcccta aaggcgatgg agcggtgcgt attcagcgtc agaccagtgg
     6241 acgtaaaggt aagggcgttt gcctgattac cggtatcgat ctcgatgatg ccgaactgac
     6301 aaaacttgca gcggaactga agaaaaaatg cggctgcggc ggagcagtta aagatggagt
     6361 tattgaaatc cagggcgata agcgtgattt attaaaatca ctacttgaag cgaaagggat
     6421 gaaggtaaaa ctcgcaggcg gttaacataa aaagccacgg atatatccgt ggctttcgaa
     6481 tattttactg tgtgtattat tcagttattt cgtactgaac agaa
//
LOCUS       DADLXX010000173         6241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLXX010000173 DADLXX010000000
VERSION     DADLXX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.176"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            7..312
                     /locus_tag="JNX27_003715"
     CDS             7..312
                     /locus_tag="JNX27_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7884749.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(388..852)
                     /locus_tag="JNX27_003716"
     CDS             complement(388..852)
                     /locus_tag="JNX27_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7884750.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /locus_tag="JNX27_003717"
     CDS             complement(849..1724)
                     /locus_tag="JNX27_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7884751.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEATASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="JNX27_003718"
     CDS             complement(1721..2410)
                     /locus_tag="JNX27_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7884752.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JNX27_003719"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JNX27_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7884753.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JNX27_003720"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JNX27_003720"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7884754.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JNX27_003721"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JNX27_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7884755.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1579 a         1691 c         1550 g         1421 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcagttgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgtggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcagcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatt
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 a
//
LOCUS       DADLXX010000174         6124 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLXX010000174 DADLXX010000000
VERSION     DADLXX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.177"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNX27_003722"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNX27_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY7884756.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="JNX27_003723"
     CDS             962..1303
                     /locus_tag="JNX27_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY7884757.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="JNX27_003724"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="JNX27_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="JNX27_003725"
     CDS             complement(1901..2611)
                     /locus_tag="JNX27_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7884758.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="JNX27_003726"
     CDS             2719..3024
                     /locus_tag="JNX27_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7884759.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..4344
                     /locus_tag="JNX27_003727"
     CDS             3223..4344
                     /locus_tag="JNX27_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7884760.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEVVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYIC"
     gene            4378..4701
                     /locus_tag="JNX27_003728"
     CDS             4378..4701
                     /locus_tag="JNX27_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7884761.1"
                     /translation="MTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLDNPV
                     KTSISCFSWTDAIDHGPQMTALRDGVRGKDKLDVPIKFIWNYKDLKRLLVRVTRDLSR
                     PEVLH"
     gene            complement(5294..>6124)
                     /locus_tag="JNX27_003729"
     CDS             complement(5294..>6124)
                     /locus_tag="JNX27_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884762.1"
                     /translation="LKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGER
                     PEVVTVPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTI
                     NARMVDVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHE
                     SQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDG
                     ENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA
                     "
BASE COUNT         1585 a         1556 c         1502 g         1481 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agttgtatat attcagtact cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gacccccggt ggcctctttg tcaggactca
     4801 gggtggcacc ggggaacatt ggtgtcttaa ttgtattgaa tgaggtctct aacttttggt
     4861 ccaaaactca tcgttagctg ctcgccagct cacagccgaa cccgaactgt aagcacgacc
     4921 accagcacca gcttcagcag gaagacgaca agatagactt tatgcaggtc catgcgccct
     4981 cctgtgtcta ttgtaccact gcactagata actagtacaa aataaagtcc agaccgaagg
     5041 cccagaccat agttaattaa tcacattgat aatccaccac ctgaagtcct gccaacattc
     5101 agtccagcct tacccatgct aggagtctta accggggtgt cgttctgtgt ctccaccact
     5161 ggtgccgcat caggctctac aggaagctcc acagcaggcg cagggaccgg aggtgatacg
     5221 gatgtaccac caccagcgga gaagccctta gcgatggacc ggaggccttt actaatgctc
     5281 ttacccattg ctgttacgct tcggtcttgg tgaacttcag gcgtttgact acaggcagga
     5341 ctgcggttgc agcgccagtg tccagcttaa ctgtcgtagc agcttcagcg acaacgaaga
     5401 ccttaccgag gatttccaag tcgtgggact gagagtcgcc atcacgtgaa aggacgaccg
     5461 taatattctc accgtcgtga cggaagcagc cgaagctcag ggccgtgaag cgtttagcca
     5521 gcatgaacac acctttgtcc tcaccgttgt aatacgcgat gtcggtaccc agcatatcca
     5581 cgatagcgtc ttcaccagag aagccctgtt tagcagccag acgggacagc gctttcagtt
     5641 gggactcatg ggcgatgatg ccgtaatcag acacttcaga gccgaaagag gactcggtgt
     5701 tctcaaacag agcgtccatg atagcgtctg ccttagtggt caggcttcca gtcaggactg
     5761 gcagctctac ggtcttcagc agcttggagg actccagaac gtcaaccatg cgagcgttga
     5821 tggtacggac cacattctca cagatgttca tagcgtcctg ctggagtgtg ccatgagtga
     5881 aaccagtgga ggacttggcg tcccacagtt tagtttgctc cgagatgagg attgcctgtt
     5941 cgataggttc gacgacaatc tcctgaccag acggaacggt cacgacttca ggtcgctcac
     6001 caccgcgtac agggacgacc acagagcggt cagacagggt gtgggcgatg attggggtag
     6061 gcaggctatt gcgggtaggc aggacttcta cagggaacgg aatgttatcc tgagcgtctt
     6121 taag
//
LOCUS       DADLXX010000175         5858 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLXX010000175 DADLXX010000000
VERSION     DADLXX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.178"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            83..442
                     /locus_tag="JNX27_003730"
     CDS             83..442
                     /locus_tag="JNX27_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7884763.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(562..741)
                     /locus_tag="JNX27_003731"
     CDS             complement(562..741)
                     /locus_tag="JNX27_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7884764.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            815..2176
                     /gene="pabB"
                     /locus_tag="JNX27_003732"
     CDS             815..2176
                     /gene="pabB"
                     /locus_tag="JNX27_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7884765.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2180..2758
                     /locus_tag="JNX27_003733"
     CDS             2180..2758
                     /locus_tag="JNX27_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7884766.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHTGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2942..4306
                     /gene="sdaA"
                     /locus_tag="JNX27_003734"
     CDS             2942..4306
                     /gene="sdaA"
                     /locus_tag="JNX27_003734"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7884767.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4437..>5858
                     /locus_tag="JNX27_003735"
     CDS             4437..>5858
                     /locus_tag="JNX27_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7884768.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAE"
BASE COUNT         1424 a         1464 c         1471 g         1499 t
ORIGIN      
        1 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccaactc gtgccatatc
       61 gttataatcc ctttgatatt agatgcaaat taaggtcata tatagcctta tcgataacat
      121 ggttaatttt aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg
      181 tgccatggat gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat
      241 ctttaaatat ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc
      301 tgaccaggaa ggctggaacc ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat
      361 ggagacaggt gaacgctctg tgattaaaaa tcctgaatac ttttcaacat atatgcagga
      421 agaactaaaa gcactggtct gagttaaatt tatatcagca taaatgggtc aggacgcttt
      481 taatcacata taaaaagcgt cctttttctc acaatcaaat gatcatcggc ttctataacg
      541 aaatcgatac ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattagctc
      601 gccggagtgg ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctactcat
      661 cccttgtgcc atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag
      721 tgaaggtaaa cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat
      781 gctacccggc acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat
      841 tactttaccc tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc
      901 gtgggcgatg cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt
      961 ggtcgccgat ccgatttgca ctttaaccac tttaggtaaa gaaaccgttg ttagtgaaag
     1021 cgaaaaacgc acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg
     1081 cgcagacatt cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt
     1141 gtttggctac gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat
     1201 cgttctgccg gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg
     1261 tcatacagtt tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag
     1321 ccagcaattc tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac
     1381 ccacgagcag tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg
     1441 ctatcaggtg aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc
     1501 attccttcag cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca
     1561 gggtgcaatt ttaagccttt cgccagagcg gtttattctt tgtgataata gtgaaatcca
     1621 gacccgcccg attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca
     1681 agcagaaaaa ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt
     1741 aatgcgtaat gatatcggtc gtgttgccgt agccggttcg gtaaaagtac cagagctctt
     1801 cgtggtggaa cccttccctg ccgtgcatca tctggtcagc actataacgg cgcgactacc
     1861 agaacagtta cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg
     1921 ggctccgaaa gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc
     1981 ctggtgcggc agcattggct atttgagctt ttgcggcaac atggatacca gcattactat
     2041 ccgcacgctg actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc
     2101 cgatagccag gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg
     2161 ccaactggag aagtaaaacg tggaataccg tagcctgacg cttgatgatt ttttatctcg
     2221 ctttcaactt ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt
     2281 gttaatcccc atcgtccgtc gaccacaacc ggggttgttg ctgactcagc gttcaattca
     2341 tctgcgtaaa cacactggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc
     2401 atcagttatc gccgccgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc
     2461 cgttgaagtt atcggcgtgc tgccgcccgt cgatagcgta actggctatc aggtaacccc
     2521 agtggtcggc attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc
     2581 ggtgtttgaa atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat
     2641 ctaccgccgt ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg
     2701 gggaatgacc gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga acccctgact
     2761 atacttatct ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat
     2821 cgcggttttt gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt
     2881 acactatgcg ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat
     2941 cgtgattagt ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt
     3001 agggcctatg aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga
     3061 tagcgttact cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca
     3121 ccacaccgat atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat
     3181 cgacagtatt cccggtttta ttcgcgacgt aaaagagcgc gaacgtctgc tgctggcaca
     3241 gggacggcat gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct
     3301 gccgctgcat gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag
     3361 caaaacttat tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga
     3421 tgctgccaac gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta
     3481 ctgtaatgaa accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca
     3541 cagcaagaaa gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat
     3601 cgatcgcggg atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc
     3661 gtctgccctg cgccggatgt tagtttccag cgataaactg tctaacgatc cgatgaatgt
     3721 cattgactgg gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg
     3781 tgtggtaact gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta
     3841 tgaccacttt attgaatcgg tcagcccaga catctatacc cgttacttta tggcagcggg
     3901 cgcgattggt gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca
     3961 gggcgaagtg ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg
     4021 tagcccggaa caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac
     4081 ctgcgacccg gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc
     4141 tgtgaaggcg attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc
     4201 gctggataag gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg
     4261 cgaaacctca cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc
     4321 ccatctgcaa cggatgggcg aatttatacc cgctttctcg tctgctgtaa tattccccac
     4381 tacacttcca ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc
     4441 aaaaagcaca acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt
     4501 gcgcactcgt tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa
     4561 atcaacaacg ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc
     4621 ttcctctaca ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg
     4681 cccatttgcc attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg
     4741 tgcaagacgg cacactttat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg
     4801 acattctggc tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc
     4861 tgattaaagg cagtccggtt ttgattcaat ggacgcctgc agcaggcagt agcaatgctg
     4921 gggtcataga gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc
     4981 cgcaaatcag tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc
     5041 tggtagattc ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaaagct
     5101 ttccttttac cattaacgta aatggtccgg aggctacggc gctggcatgg cactatcttc
     5161 caacacaatt accgctggcg gtgctgctaa gtttactggt gggctatatc gcctggctgg
     5221 cgaccgctta ccggatgagc ttttcccgcg aaattaatct gggcctggcg caacatgagt
     5281 tcgaattgtt ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga
     5341 ttttgctgcg ctggaataat ccgcgtcagg gctggatttc accggatgtg tttattccta
     5401 tcgcggaaga acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc
     5461 gtcagcgcca tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca
     5521 gccattttcg ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc
     5581 cgattcagca actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc
     5641 ggattgcccg cgagctgcat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg
     5701 gcaacagctc gttttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt
     5761 catttaccgc agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg
     5821 cgctggggca aagactgaat attgaactgg tggcggag
//
LOCUS       DADLXX010000176         5849 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLXX010000176 DADLXX010000000
VERSION     DADLXX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.179"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(136..555)
                     /locus_tag="JNX27_003736"
                     /pseudo
     CDS             complement(136..555)
                     /locus_tag="JNX27_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            605..1459
                     /gene="ltrA"
                     /locus_tag="JNX27_003737"
                     /pseudo
     CDS             605..1459
                     /gene="ltrA"
                     /locus_tag="JNX27_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1867..2046
                     /locus_tag="JNX27_003738"
                     /pseudo
     CDS             1867..2046
                     /locus_tag="JNX27_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2076..2558)
                     /locus_tag="JNX27_003739"
                     /pseudo
     CDS             complement(2076..2558)
                     /locus_tag="JNX27_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2814..3794)
                     /locus_tag="JNX27_003740"
     CDS             complement(2814..3794)
                     /locus_tag="JNX27_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7884769.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3794..4993)
                     /locus_tag="JNX27_003741"
     CDS             complement(3794..4993)
                     /locus_tag="JNX27_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7884770.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5315..5653)
                     /locus_tag="JNX27_003742"
                     /pseudo
     CDS             complement(5315..5653)
                     /locus_tag="JNX27_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5720..>5849)
                     /locus_tag="JNX27_003743"
     CDS             complement(5720..>5849)
                     /locus_tag="JNX27_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7884771.1"
                     /translation="AALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1596 a         1338 c         1391 g         1524 t
ORIGIN      
        1 ggagccgggc agcggtaaaa caaggactgc ccggctgtgg gtctacgttc gtgatgaccg
       61 taacgccggt tcggaaattg cagcataagt tatccatgct ctgaggtggg tggaggtggg
      121 ccctccggtc agggtttaca ggagcactga ccaaacgacc cggtgctgct tcattctaag
      181 gaatgaggtg gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga
      241 agctgaacga aagtaaactg tccgatgaag catcgaaatc ttagatgatg ccaaaatggg
      301 gccgtacagc cgtcccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc
      361 ggtgtgaagg cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc
      421 ggcacgtatc gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtcatgg
      481 gtctggagcg aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg
      541 acgtaactga tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat
      601 ctggatgtgc gtcgcaacaa gggagccccc cggatgcgac gggcagactt tgaaaatgtt
      661 cgaccagcaa cgtgatggca atctatacaa gatctggaac cggctatgtt cgggaacatg
      721 gtttccgcca ccagtgctgg aaaaacggat cccgaagtcg aatggcaagg aacgcattct
      781 ggggatccca acagtatccg atcgaattgc tcagggagcg ataaaacttt tcatggaaga
      841 aaagcttgac ccgattttcc atgctgattc atatggttac cgaccaggca aatcagctca
      901 tgacgctctg aaacaatgtg ccatccggtg ctggcgttat agctggatac tggaagtgga
      961 catcagtgcg tttttcgacc atgtgagaca tgatctggtg ctcaaagctc tggaacacca
     1021 cgggatgcct aaatgggtca tcctgtactg tcgacgctgg atggaagcgc caatgcagag
     1081 ttgtgaaaat ggagaattaa taacccgaac acgaggcact ccgcagggcg gggtcattag
     1141 tccgctactt gctaacttgt tccttcacta tgcatttgat ttgtggatgg aaagagaata
     1201 tcggggggta ccgtttgaga ggtacgctga cgatattgta gtacattgtt cacgaatgag
     1261 tgatgtaaca aggctaaaga acaggttatc ggagcgcttc tcggaagtcg ggctggtcct
     1321 gaatgcaggg aaaacgaata tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac
     1381 gagtttcacc tttctcggat atgacttcaa agtgcgtacg ctgaagaatt tcaaaggcga
     1441 acggtaccga aaatgcatgc cgggtgcgtc aaatgcagca atgctgcaaa ataacagaaa
     1501 caatcaagaa gtggcgtata catcgctcaa cagctgagag tttgctggat tttgcgaggc
     1561 gctacaatgc gatagtgaga ggctggatcg agtactacgg aaagttctgg tccagaaatt
     1621 tcaactatcg actgtggagt gcaatgcagt cacgtctgct caagtggatg cagtctaaat
     1681 acagactttc gaaccggaag gctcagcgaa agctgacgct ggtaaggaag gagtatccga
     1741 agctatttgt tcactggtat ttactacgtg catcaaatga gtgatcaaga gccgtatgac
     1801 gcgagagtgt cacgtacggt tccgtgagca gccggggagt gaaattctct ccggctgact
     1861 cgactcgcgg gttccgacag cgatgacgaa catgcggcgg tgttgtactc gctgatcggc
     1921 acatgccgtc tgaacaatgt ggaaccagaa aaatggctgc gttacgtcat tgagcatatc
     1981 caggactggc cggcaaaccg ggtacgtgat ctgttgccct ggaaagttga tctgagctct
     2041 cagtaaatat caatacggtt ctgacgagcc gcttactttt ggcgtaattc tgctgcgcct
     2101 ttactgaaca caatgccgta ttcagcaaga agtccacgaa tgtgatttat cagttgtgtc
     2161 ctttccattg aaaccaaccg acgcatattc aaaaaagtct ggacgtcctg ttgctccaga
     2221 gtttttattg gtacgggtcg tatagttcca tgttggcagg cctcagctat tgcctgtgcg
     2281 tcgttataat catttttctg gcaccgctga taagctttaa catgttgtgc cggaagcaat
     2341 attacatcat ggtcgagttt cctgatttcc cgggcccagt aatgagatgc cccgccagcc
     2401 tccatcgcaa cagtcatttt aggctgttgc acctgccagt taagcagttg attgcgagaa
     2461 agtttggtct ttcctgcggg tttgccatga gcattgatac tgaaaagata aaagttcgtt
     2521 ttagccagat caatgccggt gattttctgt tcagacatgg tatcccgctt acttttgtgg
     2581 ttaaagttta tccatcatgg cgcaattgaa ggctgccgtg aaagaggggg aagactatct
     2641 catcggttac tgttaaacgg ctaagagaaa ctatcttttt caagcaaaga agatacggtg
     2701 aaacaggcga tttcaccgtg aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc
     2761 ttttaaaaca gccaattaaa aactggtgag ttgctggcga aaaacttgaa ttttcacagc
     2821 gaattcagat tatcttttag tacaagagca atcatacgat ctaacttatc ctgaacttcc
     2881 aacggtaatc tgccgaattc atatgagaac gtacgtccct ttactcgttt tcgagcaaat
     2941 ttatctttac tatcaaattt ccataacaaa gttacaaccg ctttatcctt tactcctgaa
     3001 tcaagcaatg aagacatttc cttggttata agccgcataa tactgttttt aacctcgtct
     3061 tcagaaagat tgtcatcagc agtaataagc tcaatactcg gcgagatatt cttcacaact
     3121 tcatcaatag atatatcacc cagcctcagt cgttcactta attcagccag ttgcctgtaa
     3181 tcggcgtatg tcagctcaga ctgaacagga aaaagagaca caaaatcttt aggtacggac
     3241 gctgcttgga gggcacgagt caccttagct gccgacaatc cctctctttc cgcaatttgc
     3301 ttctggttca taccggcctc tttgagacgt actaaacgca acccaatttc acgaatatta
     3361 tgttcaagtg acgtctgcaa atctttcgcc agatgctgag cttcagaaac agtaagctca
     3421 tcatccgtta caagtacacg taatccaacc ttgcatagca aagctgctgc ccgacgacgg
     3481 gaaccatcca aaatctcaat aagatcgcca gtccgaatcc caatacatgg atagaattgt
     3541 tgtaaacgta tagttctggt gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg
     3601 cggccattca cctcttcaac cacaaaagtt tgagtgtcca ctttgtcatg atcaaccttg
     3661 atctcagtaa attttgcctt tcggccagta ttgagggtaa atacatgaac tggagccgat
     3721 atttcttcag tattattcag gatatttgta ttcagcgtac ggccaatagt cggtcggtgc
     3781 tttctgtttt ccatcagtag ttggccctta taaattcaat acgatcaaaa actgctttag
     3841 cgaaatcttc tgctgccata cgtgcatttt tcaaagcttc accactgcca acataagtaa
     3901 caggattcgc agatatcacg gtatcaaacg actcgccgct tctctcaaaa ccatcaagac
     3961 gtggcattga aacatcaagc atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt
     4021 atttatgatc tgacttattc gccagtttcg acatgaagcc aatatttgcc tgcaaacggc
     4081 atgaacatcc atcctgttca ataatctgta caagttcagg taaacgagcc aaatatttaa
     4141 gcgtggaatg gaaatcaact tgtgctggcg gcaccggtgt aaacatgata tctgcggcag
     4201 caatggcatt tttcaaaaat gcgtccagat gcgggcctgt atcaataagg atgaagtcga
     4261 aatcatgctt cagcttatca ataatattct cacgcaatat cgcgtgtttg ttttgtccta
     4321 atagatgctc ctcgcacaga gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag
     4381 ccggcataac ataaacgcca gggataactg acggaacgat aaaatcttcc agtaattcct
     4441 cacgactcac gttctgcaac atagcctgcg gtgcggttgt atcaaccagc cccaccgcat
     4501 gcaagtagtt cagaaacata gtggctgatg actggggatc taaatcaagg agcagaatac
     4561 gtaaatcttc tgacagcagg tgaggatgag cacgcagggc gtgagctacg gaaacactac
     4621 tcacggtttt agacacaccg ccttttagac taccgatgaa tatggaatat gcctcactat
     4681 aacgatcgcg atatttagga atgccacgat gtgcatagat atcgataatg ttctggatgg
     4741 ttaatgcata ttttgtcgcg gttcctgctg ggcgtttttc aaactgatag ccctgagcct
     4801 ccatctcacc gactgcacca tccacgcttg cgcgcgttaa ttttggaagc ttagctacag
     4861 cagactttga atagacttga taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa
     4921 tctgttttgt tagggcgagc aacattttat ctgcacgctg cgcaacctta cttaactgct
     4981 caaaactagt cattccccct ccgtgctatg taggatttta acttattaca aaaaaaactg
     5041 cattagcagg gtaatgcatg caagtacata ttgtacatgc ttatattaaa aatgcgcgca
     5101 ttgctgattt attaatcatc ggaatgaata ttactgtaag atagtatgcc tttccgggag
     5161 aaagacgctg atttcccccg aataatccag ttcaccacca cactccggac aacagctttc
     5221 ttcaggctcc agatggtgta tttcgcggga aacatgttcc ggtaacggac ggcgatgacg
     5281 tgactgtcgc aacaggcggg ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga
     5341 aggagccgat gactttttcg tggatttcca cggaacgcga gaagcagatt gttttacgtg
     5401 ccagccgctt gatgcgggtg cggagggtcc ggttgttacg ttcaatacgt tgcgtgaaga
     5461 ttttgccggt caggtgtttc tccttcggca cttctctggc gtagctgccc cagtcatcgc
     5521 tggtcaccat gccgatacag aacggagtca acagggctaa cagttcccga caggtctcat
     5581 cattccgtgg cccgaaagtg taagccaaca caccgccagt tttagtgttg tacgcatacc
     5641 agagccagtg ttgtgtcaac gacggatgaa aagtgctcca cttatatctc caccaacggc
     5701 ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg gcctttcgtt
     5761 tctgtctgag tcgatagctt tctcctttga tttgaacgac atgtgagtgg tgtaagatac
     5821 ggcccagcat cgctgaggtc agtgctgca
//
LOCUS       DADLXX010000177         5805 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLXX010000177 DADLXX010000000
VERSION     DADLXX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.180"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JNX27_003744"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JNX27_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7884772.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JNX27_003745"
     CDS             578..1027
                     /locus_tag="JNX27_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7884773.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1260)
                     /locus_tag="JNX27_003746"
                     /pseudo
     CDS             complement(1028..1260)
                     /locus_tag="JNX27_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1346..1519)
                     /locus_tag="JNX27_003747"
     CDS             complement(1346..1519)
                     /locus_tag="JNX27_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7884774.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1714..1809
                     /gene="yncL"
                     /locus_tag="JNX27_003748"
     CDS             1714..1809
                     /gene="yncL"
                     /locus_tag="JNX27_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7884775.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2211..3020)
                     /locus_tag="JNX27_003749"
     CDS             complement(2211..3020)
                     /locus_tag="JNX27_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY7884776.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNX27_003750"
                     /pseudo
     CDS             complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNX27_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4677..5471)
                     /locus_tag="JNX27_003751"
     CDS             complement(4677..5471)
                     /locus_tag="JNX27_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7884777.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="JNX27_003752"
     CDS             complement(5461..>5805)
                     /locus_tag="JNX27_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7884778.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1470 c         1289 g         1524 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg tgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt ccatatcttc
     1081 tttatacttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     1141 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     1201 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     1261 actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg
     1321 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     1381 tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     1441 gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     1501 tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat
     1561 gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag
     1621 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     1681 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     1741 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     1801 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac
     1861 agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat atttacttct
     1921 ttttgctggc atctacattg aaatgagtag aaaatatccc cctactgtcg tctatacaaa
     1981 cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac
     2041 gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc
     2101 gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat
     2161 tgcaaacccc gccctggtta tcttgcccat gtatcatgca aaacagataa ttataggcct
     2221 ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat
     2281 atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt attatccgga
     2341 agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa
     2401 tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca
     2461 tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct
     2521 ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg
     2581 gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat
     2641 tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt
     2701 atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg
     2761 caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat
     2821 tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa
     2881 ttcctttctt tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa
     2941 aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat
     3001 ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt ttaataacga
     3061 tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta
     3121 gcctaatttt tacgccagtt attaatgtaa tcattctaat acttaaccgc cattaaactc
     3181 agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa
     3241 ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt
     3301 aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga
     3361 cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg
     3421 tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt
     3481 cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc acgatggcat
     3541 cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta cccttgcgct
     3601 tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc
     3661 tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac tcgtcatccg
     3721 gcgcaccaga ttttaacgtt gccaccgcag gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaaaata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatgc ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 accagttctc cgttaatcag taacttatgt tgcatagatt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 tagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DADLXX010000178         5723 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLXX010000178 DADLXX010000000
VERSION     DADLXX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.181"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            55..1515
                     /locus_tag="JNX27_003753"
     CDS             55..1515
                     /locus_tag="JNX27_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7884779.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVAEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="JNX27_003754"
     CDS             complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="JNX27_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7884780.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1932..2643)
                     /locus_tag="JNX27_003755"
                     /pseudo
     CDS             complement(1932..2643)
                     /locus_tag="JNX27_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="JNX27_003756"
     CDS             complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="JNX27_003756"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7884781.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3615..5660
                     /gene="dcp"
                     /locus_tag="JNX27_003757"
     CDS             3615..5660
                     /gene="dcp"
                     /locus_tag="JNX27_003757"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7884782.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1430 a         1361 c         1480 g         1452 t
ORIGIN      
        1 tttattcata taacaaaaca tatagcttgc catatttaaa tttaaggaat tgttatggga
       61 aataatttgt tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca
      121 agaattgttc atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat
      181 attcttgcta cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc
      241 gaacagcaaa ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg
      301 gccgatgcgt ggacggctcg cgtcgttggc gtcgttaaaa aagccttgca cgtacagatg
      361 gatggcttag aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg
      421 acaatcaccg aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac
      481 ccgatggtcg ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt
      541 gttgaggcgc tggctcgccg taaagcggca ggacttcccg catttaccgt catgtcatgt
      601 gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta cgcgcaagcc
      661 gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg
      721 gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca acttaccggt
      781 gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat cgaagataac
      841 tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag tgatgtgctg
      901 ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcatatctg
      961 ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca ttatcgtcat
     1021 gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat
     1081 ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt acgtcatcga
     1141 acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt
     1201 cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg
     1261 atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgacccactg
     1321 ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca
     1381 ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt gtttactgca
     1441 aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa
     1501 tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac ttagttcatg
     1561 ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt
     1621 tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca
     1681 gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga
     1741 tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg
     1801 cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt
     1861 ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg gatgggatga
     1921 gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat ctggcgcacg
     1981 gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt
     2041 ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg tggagaaaca
     2101 tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc aatggtatcc
     2161 cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca
     2221 aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc
     2281 agttggtagc actggctttc ggtaatcatg ctcgcactgg ctttcggtaa tcatgctcgc
     2341 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2401 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     2461 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     2521 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     2581 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     2641 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2701 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     2761 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     2821 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     2881 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     2941 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     3001 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     3061 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3121 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     3181 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3241 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     3301 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     3361 actcctgcaa ccgctcctgg cgacgcccag tggcgataac tttatgccct tgttgaataa
     3421 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3481 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3541 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3601 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     3661 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3721 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     3781 acaatactat tctggcactg gaacaaagcg gagaattaat tacccgcgtt accagcgtct
     3841 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     3901 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     3961 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4021 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     4081 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     4141 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4201 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4261 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     4321 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4381 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4441 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4501 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     4561 tagcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     4621 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     4681 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     4741 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     4801 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     4861 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     4921 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     4981 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     5041 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5101 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5161 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5221 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5281 ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt
     5341 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5401 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5461 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5521 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5581 tggaacgcct gcatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5641 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5701 ctggttatag cagtaattgc agc
//
LOCUS       DADLXX010000179         5630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLXX010000179 DADLXX010000000
VERSION     DADLXX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.183"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            34..435
                     /locus_tag="JNX27_003758"
     CDS             34..435
                     /locus_tag="JNX27_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY7884783.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JNX27_003759"
     CDS             447..818
                     /locus_tag="JNX27_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884784.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="JNX27_003760"
     CDS             805..1395
                     /locus_tag="JNX27_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884785.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="JNX27_003761"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="JNX27_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="JNX27_003762"
     CDS             1802..2542
                     /locus_tag="JNX27_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884786.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="JNX27_003763"
     CDS             2601..2987
                     /locus_tag="JNX27_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY7884787.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="JNX27_003764"
     CDS             2996..3325
                     /locus_tag="JNX27_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY7884788.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..5630
                     /locus_tag="JNX27_003765"
                     /pseudo
     CDS             3297..5630
                     /locus_tag="JNX27_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         1387 a         1306 c         1784 g         1153 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc
//
LOCUS       DADLXX010000180         5599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLXX010000180 DADLXX010000000
VERSION     DADLXX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.184"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..410
                     /locus_tag="JNX27_003766"
     CDS             <1..410
                     /locus_tag="JNX27_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7884789.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /locus_tag="JNX27_003767"
                     /pseudo
     CDS             442..1056
                     /locus_tag="JNX27_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNX27_003768"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNX27_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY7884790.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSIVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1402 c         1329 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actatacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DADLXX010000181         5583 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLXX010000181 DADLXX010000000
VERSION     DADLXX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.185"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            29..244
                     /locus_tag="JNX27_003769"
     CDS             29..244
                     /locus_tag="JNX27_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7884791.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="JNX27_003770"
     CDS             237..623
                     /locus_tag="JNX27_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7884792.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="JNX27_003771"
     CDS             631..1317
                     /locus_tag="JNX27_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7884793.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="JNX27_003772"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="JNX27_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7884794.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="JNX27_003773"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="JNX27_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7884795.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="JNX27_003774"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="JNX27_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7884796.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3999..4409
                     /locus_tag="JNX27_003775"
                     /pseudo
     CDS             3999..4409
                     /locus_tag="JNX27_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4425..>5583)
                     /locus_tag="JNX27_003776"
     CDS             complement(4425..>5583)
                     /locus_tag="JNX27_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY7884797.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1543 a         1030 c         1215 g         1795 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
     5221 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
     5281 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
     5341 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
     5401 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
     5461 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
     5521 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
     5581 aat
//
LOCUS       DADLXX010000182         5576 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLXX010000182 DADLXX010000000
VERSION     DADLXX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.186"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..87)
                     /locus_tag="JNX27_003777"
     CDS             complement(<1..87)
                     /locus_tag="JNX27_003777"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7884798.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNX27_003778"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNX27_003778"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7884799.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSSPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGEGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JNX27_003779"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JNX27_003779"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY7884800.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1730 a         1256 c          935 g         1655 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctctc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact gaaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggctg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aattttatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
     5461 aacaggatga ctaaaaatac acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg
     5521 gttctggaaa gtcagggcga atataactca caatgggcag cgatttgttc cattgc
//
LOCUS       DADLXX010000183         5566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLXX010000183 DADLXX010000000
VERSION     DADLXX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.187"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..268)
                     /locus_tag="JNX27_003780"
     CDS             complement(<1..268)
                     /locus_tag="JNX27_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7884801.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /locus_tag="JNX27_003781"
     CDS             805..2706
                     /locus_tag="JNX27_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY7884802.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JNX27_003782"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JNX27_003782"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7884803.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JNX27_003783"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JNX27_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7884804.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JNX27_003784"
     CDS             complement(4548..4757)
                     /locus_tag="JNX27_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884805.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JNX27_003785"
     CDS             5041..5403
                     /locus_tag="JNX27_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7884806.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1409 a         1383 c         1436 g         1338 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aagccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaata
//
LOCUS       DADLXX010000184         5537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLXX010000184 DADLXX010000000
VERSION     DADLXX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.188"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JNX27_003786"
                     /pseudo
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JNX27_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JNX27_003787"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JNX27_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7884807.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5537)
                     /locus_tag="JNX27_003788"
     CDS             complement(2659..>5537)
                     /locus_tag="JNX27_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7884808.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGISYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1463 a         1286 c         1302 g         1486 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgtag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gatgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catagcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtcttttata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaag
//
LOCUS       DADLXX010000185         5531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLXX010000185 DADLXX010000000
VERSION     DADLXX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.189"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            100..1008
                     /locus_tag="JNX27_003789"
     CDS             100..1008
                     /locus_tag="JNX27_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY7884809.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNX27_003790"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNX27_003790"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7884810.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /locus_tag="JNX27_003791"
     CDS             3323..4081
                     /locus_tag="JNX27_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7884811.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNX27_003792"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNX27_003792"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7884812.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="JNX27_003793"
     CDS             complement(5343..>5531)
                     /locus_tag="JNX27_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884813.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1323 a         1484 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tccacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag ccaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gataccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DADLXX010000186         5392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLXX010000186 DADLXX010000000
VERSION     DADLXX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.190"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..446)
                     /locus_tag="JNX27_003794"
     CDS             complement(<1..446)
                     /locus_tag="JNX27_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7884814.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /locus_tag="JNX27_003795"
     CDS             complement(501..1352)
                     /locus_tag="JNX27_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7884815.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALSAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /locus_tag="JNX27_003796"
     CDS             complement(1365..2165)
                     /locus_tag="JNX27_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7884816.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNX27_003797"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNX27_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7884817.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="JNX27_003798"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="JNX27_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7884818.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFFFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..5389)
                     /locus_tag="JNX27_003799"
     CDS             complement(5216..5389)
                     /locus_tag="JNX27_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rtn protein"
                     /protein_id="HAY7884819.1"
                     /translation="METQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPA
                     RHNGHITPIMSLR"
BASE COUNT         1311 a         1362 c         1373 g         1346 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcact cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttcttcttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg cc
//
LOCUS       DADLXX010000187         5337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLXX010000187 DADLXX010000000
VERSION     DADLXX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.191"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNX27_003800"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNX27_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7884820.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JNX27_003801"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JNX27_003801"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7884821.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JNX27_003802"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JNX27_003802"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7884822.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JNX27_003803"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JNX27_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7884823.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1456 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggt
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADLXX010000188         5294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLXX010000188 DADLXX010000000
VERSION     DADLXX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.192"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..583
                     /locus_tag="JNX27_003804"
                     /pseudo
     CDS             1..583
                     /locus_tag="JNX27_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            647..1573
                     /gene="glsB"
                     /locus_tag="JNX27_003805"
     CDS             647..1573
                     /gene="glsB"
                     /locus_tag="JNX27_003805"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7884824.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1573..1932
                     /locus_tag="JNX27_003806"
     CDS             1573..1932
                     /locus_tag="JNX27_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7884825.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2071..3489
                     /locus_tag="JNX27_003807"
     CDS             2071..3489
                     /locus_tag="JNX27_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7884826.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3716..5167
                     /locus_tag="JNX27_003808"
     CDS             3716..5167
                     /locus_tag="JNX27_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY7884827.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1224 a         1234 c         1422 g         1414 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gcactgaaaa aatgcgtact actatccgcc
      241 aacggttctg gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc
      301 cgttgctgca atcaccattg cgaaagatgc agaacatgcg ctggaactgg ctaatgatag
      361 tgagttcggc ctttcagcga ccatttttac caccgacgaa acacaggcca gacagatggc
      421 ggcacgtctg gaatgcggtg gggtgtttat caatggttat tgtgccagcg acgcgcgagt
      481 ggcctttggt ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt tcggcttaca
      541 cgaattctgt aatatccaga cagtgtggaa agaccggatc tgacgcgaca tattcagctc
      601 tgatatactc gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg cagtcgccat
      661 ggataatgca attttagaaa acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa
      721 agtcgcggat tatattccgg cgctggctac agtagacggt tcccgattgg ggattgctat
      781 ctgtaccgtt gacggacagc tttttcaggc cggagacgcg caagaacgtt tttccattca
      841 gtctatttcc aaagtgctga gtctcgttgt cgccatgcgt cattactccg aagaggaaat
      901 ctggcaacgc gtcggcaaag atccgtctgg atcaccgttc aattctttag tgcaactgga
      961 aatggaacag ggtattccgc gtaatccgtt cattaatgcc ggtgcgctgg tggtctgcga
     1021 tatgttgcaa gggcgactaa gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt
     1081 aagcggtgtg tctgatattt catacgatac ggtggtagcg cgttccgaat ttgaacattc
     1141 cgcgcgaaat gcggctatcg cttggctgat gaagtcgttt ggcaatttcc atcatgacgt
     1201 gacaaccgtt ctgcaaaact actttcatta ctgcgctctg aaaatgagct gtgtagagct
     1261 ggcccggacg tttgtctttc tggctaatca ggggaaagct attcatattg atgaacctgt
     1321 ggtgacgcca atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca
     1381 gaacgcgggg gagtttgcct ggcgggtggg actaccggcg aaatctggcg ttggtggcgg
     1441 tattgtggcg attgttccgc atgaaatggc catcgcagtc tggagtccgg aactggatga
     1501 tgcaggtaac tcgcttgcgg gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg
     1561 ttcggtttat taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt
     1621 cgctctcgct ttcgtctggg catgaaagag cgtcagtatt gcctggagaa aggcgcgcca
     1681 gtcatcgaac aacatgcggc ggattttgtc gctaaacgtc ttgctccggc attaccggct
     1741 aatgacggta agcaaccccc catgcgcggg catccggtgt ttatcgctca gcacgccacc
     1801 gcgacctgct gtcgcggttg tctggctaag tggcataaca ttccgcaggg tgtttcgtta
     1861 agcgaagaac aacaacgtta tatcgtggcg gtaatctatc actggctggt tgtacagatg
     1921 aatcagccat gagaattgcg gaaaacgata aaatagcatt cccagggtac ctttatttgc
     1981 cgccgctgtt agcgaacatg ctatccttta tgagaattta cttatcacgt tgagataatt
     2041 gccacccgca attgtatgga cagataatta atgcatgtac accccatttc aactttccgt
     2101 ttgttccagg aaggtcatct gctacgtaat agcatcgcta tttttgcgct aaccacgctg
     2161 ttctatttta ttggtgcaga gttacggctg gttcacgaac tttctctttt ctggccgctg
     2221 aatggcgtaa tggcgggggt gtttgcccgc tatgtctggc ttaatcgact gcattactat
     2281 gcgatcagtt atgtggcgat gctggtttat gatgccataa ccaccgaatg gggactggtt
     2341 tcactggtta tcaatttctc caatatgatg tttattgtta ccgtcgcctt actggtcgtg
     2401 cgggataagc gtctggggaa aaataagtat gagccagtaa gtgccttacg gctatttaat
     2461 tactgtctga ttgccgcatt attatgcgct attgtcgggg cgattggttc ggtcagtatt
     2521 gatagtctgg atttctggcc tttgcttgcg gactggttca gtgagcaatt ctcaacgggc
     2581 gtgttgatcg tgccttgtat gctgacgttg gctattcctg gagtgctgcc gcgctttaaa
     2641 gcagaacaga tgatgcccgc catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt
     2701 ggcggagcgg ggagtctggc gttcccgctg cctgcattaa tctggtgtgc agtgcgctat
     2761 acgccgcagg taacatgtct gttgaccttt gtcaccggtg cggtggaaat cgtactggtg
     2821 gcaaattcgg tgattgatat ctcggtcggt tcgccgttct ccattccgga aatgttctcc
     2881 gcacgtctcg gtattgccac gatggcgata tgcccaatta tggtttcttt tagcgtggca
     2941 gcgatcaatt tgctaatgaa gcaagttgcg ctgcgagccg actttgattt tctgactcag
     3001 gtttactcac ggtccggtct ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa
     3061 catctgacgg tcatgctgct tgatatcgac tatttcaaaa gcattaacga taactatgga
     3121 catgaatgtg gcgataaagt gttaagcgtg tttgcccggc atattcagaa gattgtcggt
     3181 gataaggggc tggtggcgcg catgggcggc gaagaatttg ctgttgcagt gccgtcggtg
     3241 aatcctgtcg atggtctgct aatggcggaa aaaatccgta aaggcgttga actgcaacca
     3301 ttcacctggc aacaaaaaac gctttatctc acggtaagta ttggcgtcgg tagtggtcgc
     3361 gcatcgtacc gaacgctgac cgatgatttt aataaattga tggtcgaagc cgatacgtgt
     3421 ctgtatcgct cgaagaaaga tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa
     3481 gttgtctgac atctttattc attccgcgcg atttgaatgc aatgtgttct accactttat
     3541 gatccaggaa aaggcgtatt tcctcgatta aaccatgatc cgcgccacac ttttctcata
     3601 aaactgctca ttaatcatca ggagttgatc aaaacagggg ttcggcgtat taaagtttgc
     3661 taacctgtcg caccaattca aacaggttgt atgactaatc agaagggaac ccattgtgaa
     3721 aacactaaat cgtcgcgatt ttcccggtgc acagtatcca gaacgtatca ttcagtttgg
     3781 tgaaggtaac ttcctgcgcg cctttgttga ctggcaaatc gatctcctga atgagcacac
     3841 cgatctgaat tctggcgtgg tcgttgttcg tccgattgaa acttcattcc cgccgtcact
     3901 gagcacgcag gatggtctgt acaccaccat tatccgtggc ctgaatgaga agggggaagc
     3961 ggtcagcgac gcgcgtctga ttcgctcggt taatcgtgaa atcagcgtct acagtgaata
     4021 cgatgaattc ctgaaactgg cgcacaaccc ggaaatgcgc tttgttttct ctaacaccac
     4081 cgaagcgggt atcagctacc atgcgggcga taaattcgat gacgcgccag cggtaagcta
     4141 tccggcaaaa ctgacccgtc tgctgttcga acgctttagc catttcaacg gtgcgctgga
     4201 taaaggttgg atcatcattc cgtgtgagtt gattgactac aatggcgacg cgttgcgtga
     4261 actggtgctg cgctatgcgc aagagtgggc actgccagaa gcatttattc aatggctgga
     4321 tcaggctaac agcttctgtt ctacgctggt ggaccgtatc gttaccggtt atccgcgcga
     4381 tgaagtggct aaactggaag aagaactggg ttatcacgat ggtttcctcg acaccgctga
     4441 acacttttac ctgtttgtga ttcaggggcc gaaatcctta gcgactgagc tgcgcctgga
     4501 caaatatccg ctcaacgtgc tgattgttga cgatattaaa ccgtataaag aacgcaaagt
     4561 ggcaatcctc aacggtgcgc acaccgcgct ggtgccggtg gcgttccagg cagggctgga
     4621 taccgtaggt gaagcgatga acgatgctga aatttgcgca ttcgttgaaa aagccatcta
     4681 cgaagaaatt attccggtac tggatttgcc tcgtgatgaa ctggaatctt tcgccagtgc
     4741 agtcaccggg cgtttccgta acccgtacat taagcatcag ctgctgtcta tcgcgctcaa
     4801 cggtatgacc aagttccgca cccgcatcct gccgcagctg ctggcagggc agaaggcaaa
     4861 cggcacactt ccggcgcgcc ttactttcgc attagcggca ttgattgcgt tctatcgtgg
     4921 tgagcgtaac ggagaaacat atccggtaca ggacgatgca cactggctgg aacgttatca
     4981 gcaactctgg agccagcatc gtgaccgtgt tatcggtacc caggaactgg tagcgattgt
     5041 gttggcagaa aaagaccact gggagcagga cctgacgcaa gtgcctggtc tggtcgagca
     5101 ggttgctaat gacctggatg cgattcttga aaaagggatg cgcgaagctg tacgtccgtt
     5161 gtgctaataa caccgttact tctgaacccg gtttcgaccg ggtttcttat ttcactatat
     5221 tagttacaac atcctatatg cctttatcat taacaggtgt cacatgcagc gcctgttaat
     5281 gttgtaagct tgac
//
LOCUS       DADLXX010000189         5265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLXX010000189 DADLXX010000000
VERSION     DADLXX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.193"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..166
                     /locus_tag="JNX27_003809"
     CDS             <1..166
                     /locus_tag="JNX27_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884828.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            349..2529
                     /locus_tag="JNX27_003810"
     CDS             349..2529
                     /locus_tag="JNX27_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7884829.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2670..3953)
                     /locus_tag="JNX27_003811"
     CDS             complement(2670..3953)
                     /locus_tag="JNX27_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7884830.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4088..5158)
                     /locus_tag="JNX27_003812"
     CDS             complement(4088..5158)
                     /locus_tag="JNX27_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7884831.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKIFASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5136..>5265)
                     /locus_tag="JNX27_003813"
     CDS             complement(5136..>5265)
                     /locus_tag="JNX27_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7884832.1"
                     /translation="FPPPVKDGREYLFHESAVKVDLNRPVTGSLLKRIRNGKKAKS"
BASE COUNT         1253 a         1290 c         1331 g         1391 t
ORIGIN      
        1 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
       61 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
      121 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
      181 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
      241 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
      301 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
      361 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
      421 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
      481 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
      541 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
      601 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      661 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      721 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg
      781 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      841 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
      901 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
      961 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     1021 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     1081 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     1141 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     1201 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
     1261 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     1321 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     1381 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     1441 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1501 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctagc caaaaaaggt
     1561 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     1621 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     1681 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgctgatg
     1741 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1801 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1861 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1921 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1981 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     2041 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     2101 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     2161 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     2221 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     2281 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     2341 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     2401 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     2461 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat
     2521 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     2581 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2641 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     2701 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2761 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2821 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2881 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     2941 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     3001 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     3061 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     3121 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     3181 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3241 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacact atcgcccagc
     3301 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
     3361 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3421 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3481 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3541 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
     3601 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     3661 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     3721 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     3781 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     3841 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     3901 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3961 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     4021 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     4081 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     4141 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     4201 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     4261 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     4321 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     4381 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgaa gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaat
//
LOCUS       DADLXX010000190         5144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLXX010000190 DADLXX010000000
VERSION     DADLXX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.194"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..253
                     /locus_tag="JNX27_003814"
                     /pseudo
     CDS             1..253
                     /locus_tag="JNX27_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="JNX27_003815"
     CDS             746..1492
                     /locus_tag="JNX27_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7884833.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="JNX27_003816"
     CDS             1507..1929
                     /locus_tag="JNX27_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7884834.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="JNX27_003817"
     CDS             1987..2343
                     /locus_tag="JNX27_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884835.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="JNX27_003818"
     CDS             2436..2654
                     /locus_tag="JNX27_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY7884836.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="JNX27_003819"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="JNX27_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3683
                     /locus_tag="JNX27_003820"
     CDS             3018..3683
                     /locus_tag="JNX27_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884837.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3683..4048
                     /locus_tag="JNX27_003821"
     CDS             3683..4048
                     /locus_tag="JNX27_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7884838.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4446..4658
                     /locus_tag="JNX27_003822"
     CDS             4446..4658
                     /locus_tag="JNX27_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7884839.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            4895..>5144
                     /locus_tag="JNX27_003823"
     CDS             4895..>5144
                     /locus_tag="JNX27_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884840.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT         1407 a         1087 c         1421 g         1229 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     4561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     4681 cctcgccacc tctcatgtgt caggcatcct caacgcaccc gcactaaacc cgcttcggcg
     4741 ggttttttgt tgcgtgctga atacgcaggg tgaaaaataa ccatatattt gattatatac
     4801 acaacaaaaa ataaaagtca ttgtacttgc acattaaaca atcaaatata cggcgtgaaa
     4861 taaatatttt tcagattaat atttttgtct ctatgtggat ataaccgttt gtacttataa
     4921 acctggaggc atcgtggaaa aaataaagaa actatttagt agcaaatacg cagtcatacg
     4981 tcgtgatgac ctgtcagtta tagtcgaaat ggattacttc cctgaaaccc caaaatcaat
     5041 aatgtatcgt aatggtcgaa aggcaatttt tttaccgatg agggtaagtg acattatggg
     5101 aaatgataaa ctgctggatg aattgcgagt cagagcatcc tgtt
//
LOCUS       DADLXX010000191         5128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLXX010000191 DADLXX010000000
VERSION     DADLXX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.195"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..238
                     /locus_tag="JNX27_003824"
     CDS             <1..238
                     /locus_tag="JNX27_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884841.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            278..818
                     /locus_tag="JNX27_003825"
                     /pseudo
     CDS             278..818
                     /locus_tag="JNX27_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            885..2303
                     /locus_tag="JNX27_003826"
     CDS             885..2303
                     /locus_tag="JNX27_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7884842.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHVVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2284..2754
                     /locus_tag="JNX27_003827"
     CDS             2284..2754
                     /locus_tag="JNX27_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7884843.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2743..3663)
                     /gene="yedA"
                     /locus_tag="JNX27_003828"
     CDS             complement(2743..3663)
                     /gene="yedA"
                     /locus_tag="JNX27_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7884844.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGEIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3836..4753
                     /locus_tag="JNX27_003829"
     CDS             3836..4753
                     /locus_tag="JNX27_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7884845.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAAWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4832..5014
                     /locus_tag="JNX27_003830"
     CDS             4832..5014
                     /locus_tag="JNX27_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7884846.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1225 c         1465 g         1186 t
ORIGIN      
        1 gtgggctttc tatacatggt gtggctattt cgtctgggca atggcgcgat acatctgggt
       61 gatgagccgg atccccgatg cccccgtgtc gggatttgaa agtgatttag gatctaccgc
      121 ggggaagtgg ttaggagcat tggtcggatt tctgtttatg gccctggttg gtgccttgtt
      181 aggcggcatt gcatggtaca cccgacctcg tcccgcacgt tgcagacgct atgaataaaa
      241 actgctaagg ttgcgactct ttggcaacgg agcgcgtatg gagttacaac actggcaggc
      301 acagttcgag aactggttga aaaaccatca tcaacatcag gacgcggcgc atgacctgtg
      361 tcattttcgc cgtgtctggg ctacggcaca gaaactggcg gcagatgacg atgtcgatat
      421 gctggtgatt ttaaccgcct gttattttca cgatatcgta agcctggcga aaaatcatcc
      481 gcaacggcag cgttcttcaa tcctggcggc agaagagacg cgtcgcctgc tccgtgaaga
      541 gttcgtgcaa tttccggcgg agaaaattga ggccgtttgt catgccattg ccgctcacag
      601 tttcagcgcg caaatcgccc ccttaacaac ggaagctaaa atcgtccagg atgcggatcg
      661 gctggaagcc ctgggggcga ttggcctggc gcgcgtattt gccgtttcag gggcattgag
      721 cttggcgctg tttgatggcg aagctccgtg ccgaactggc gggggagaat gaaggtatcg
      781 atcacaaggt gatagatgcg ttttcacccg ctggctgagc gcgtggccct aaacggcagt
      841 aattatgtta acctgtcggc catctcagat ggccggttaa atctatgcag gaaaatatat
      901 cagtaaccga ttcatacagc accgggaatg ccgcacaggc aatgctggag aaactgctgc
      961 aaatttatga tgttaaaatg ctggtggcgc agcttaatgg tgtgggtgag aatcactgga
     1021 gcgcggcaat tttaaaacgt gcgctggcga atgactcggc atggcaccgt ttaagtgaga
     1081 aagagtttgc ccatctgcaa acgttgttac ccaaaccacc ggaacatcat ccgcattatg
     1141 cgtttcgctt tatcgatcta tttgccggaa ttggcggcat ccgtcgtggt tttgaatcga
     1201 ttggtggaca gtgcgtgttt accagcgaat ggaacaaaca tgtggtacgc acttataaag
     1261 ccaaccatta ttgcgacccg gcgacgcatc attttaatga agatatccgc gacatcaccc
     1321 tcagtcatca agaaggcgtg agtgatgagg cggcggcgga acatattcgt caacacattc
     1381 ctgaacacga tgttttactg gccggtttcc cttgtcagcc attttcgctg gctggcgtat
     1441 cgaaaaagaa ctcgctcggg cgggcgcacg gttttgcctg cgatacccag ggcacgctgt
     1501 tctttgatgt ggtgcgcatt atcgacgcgc gtcgtccggc gatgtttgtg ctcgaaaacg
     1561 tcaaaaacct gaagagtcac gataagggaa aaacgttccg catcatcatg cagacgctgg
     1621 acgaactggg ctatgacgtt gccgatgcag aagataacgg gccggacgat ccgaaaatca
     1681 tcgatggtaa acatttcctg ccgcagcacc gtgaacgcat cgtgctggtg ggttttcgtc
     1741 gcgatctgaa tctgaaagcc gattttactc tgcgtgatat cagcgaatgt ttccctgcac
     1801 agcgagtgac gctggcgcag ctgctggacc cgatggttga ggcgaaatat atcctgacgc
     1861 cggtgctgtg gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc ggtaacggct
     1921 tcggttatgg aatggtttat ccgaacaatc cgcaaagcgt cacccgtacg ctgtctgctc
     1981 gttattacaa agatggcgcg gaaattttaa tcgatcgcgg ttgggatatg gccacgggtg
     2041 agaaagactt tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta acgcctcgtg
     2101 aatgcgcgcg cttaatgggt tttgaagcgc cgggagaagc gaaattccgc attccggttt
     2161 cggacactca ggcctatcgc cagttcggca actcggtggt cgtgccggtc tttgccgcgg
     2221 tggcaaaact gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag caagaggcac
     2281 aacatggtcg acgttcacga taaggccacc cgcagcaaaa atatgcgcgc gattgccacg
     2341 cgtgatacgg cgattgagaa gcgcctcgcc agtttgttaa ccgggcaggg ccttgcgttt
     2401 cgcgttcagg acgccagtct gcccggacgc ccggattttg tcgttgatga atatcgctgc
     2461 gtgatattta cccacggctg cttctggcat catcatcact gctatctgtt taaagtgcct
     2521 gcgactcgaa ctgagttctg gctggagaag ataggtaaaa atgttgaacg cgatcgccgc
     2581 gatatcagtc gcttgcagga gctcggctgg cgcgtactga ttgtctggga gtgtgcgtta
     2641 cgtaggcgcg agaagctgac ggatgcggcg cttaccgagc gtctggaaga gtggatctgc
     2701 ggcgaaggtg ccagcgcgca gatcgacacg caggggattc atttactcgc ttgatgcatc
     2761 ctgaataact ggcgcgacaa cgggtttggc cgggaagaga tattttccca acgtgaccag
     2821 taccaccgcg aaaacaatta cgccgagcgc cagccattca atcttcgaca gcgtttcgcc
     2881 acccagtccc gtacccagca agaccgcgac caccgggtta acgtaggcgt agctggtggc
     2941 gagagccgga ctgacattac ggattaaata catataagcg ttaatggcga taatcgaacc
     3001 aaacagcgcc agatagccga ccgcaaggaa gcctgacagg gaagggagcg ccgtcagttt
     3061 ttcacccgca atcatcgatg cgatcattaa caccacgcct gccgccagca tctcaatctc
     3121 acccgccatc atccctacag gtaaggtaat gcgcgagcca taaactgagc caaacgccca
     3181 gctaatcgag ccgattaaaa tcagaatcgc gccccacgga ttgccgctta aatttccacc
     3241 gctattgagc atgatgattc cggcaagccc aatggcaata cccacccatt ccagtttgcg
     3301 cgttttaatg ccaaacaggc gactgaagca cagggtaaag aggggcacgg ttgcaatcac
     3361 tacggcggcg atgccggaag gaacattttg atgttcggca accgtcacca tgccattacc
     3421 gacagccagc aataacaggc caatcagcgc ggcattgagc agcggacgta gcgggggaag
     3481 tttgtgtccg cgcagtagca aaaatgccag caataaaata ccggctgcca ggaatcgaac
     3541 gcccgccatc attaacggag gccagcttcc cacgccaatc cgaatgacaa aataggttga
     3601 gccccaaatg atatacaacg caaacagagc gccaaaaagc ggtaacaact ggcggaaacg
     3661 cataatccct cacggcggaa ataaaaaggt ggttcatagt aaacgtgaaa atcattctgc
     3721 tggcgagaga tataattgca cttgattgtt aaataaatgt tgacctgtga agcaagtatc
     3781 agagctgccg tttttgcttc atacttacac ccttcagcaa taaaaatgag agggaatgct
     3841 tttggccggg agtagtttac tgacgttgct cgatgatatc gccacactgc tggacgatat
     3901 ctctgtgatg ggcaaactgg cggcgaagaa aaccgccggt gtattagggg atgatttatc
     3961 gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg gaacttcccg tggtctgggg
     4021 cgtggcgaaa ggatcgctga tcaataaagt gattctggtg ccgctggcgc tgatcatcag
     4081 tgcgtttatc ccgtgggcga ttacgccact gttgatgatt ggtggcgcgt ttctctgctt
     4141 tgaaggggta gagaaagtgc tgcatatgct ggaggcgcgt aaacataaag aagatccagc
     4201 gcagagccag cagcgtctgg agaagctggc ggcgcaggat ccgctgaagt ttgaaaagga
     4261 caaaattaaa ggggcgattc gtaccgattt tattttgtct gcggaaatcg tcgccatcac
     4321 gctggggatt gtggcagagg cgccgttgct taatcaggtg ctggtgcttt caggcatcgc
     4381 gctggtagtg accgtgggcg tctacggtct ggtaggggtt atcgttaaga ttgatgacct
     4441 agggtattgg ctggcggaaa aatccagcgc gctgatgcag gcattaggta aagggttatt
     4501 gattatcgcg gcttggctga tgaaagcatt atcgattgtc ggcacgctgg cgatgttcct
     4561 cgtcggcggc gggattgtgg tacatggtat tgcgccgctg catcacgcta ttgaacatat
     4621 cgccgggcag caaagtgcag tggtggcgat gatattaccg actgttttaa atctgattct
     4681 tggatttatc atcggcggca tcgtggtgct gggagtgaaa gcagtagcga aaatgcgcgg
     4741 tcaggcacat taatactcgc actggctatt atgcaaaatt cgccatcttc aactaaggtg
     4801 aacaagtttc actcgaaaaa aggaggcgag tatgagcttt atggttagtg aggaagttac
     4861 ggttaaagag ggcggcccgc gaatgattgt cactggatac tccagcggta tggttgagtg
     4921 tcgttggtat gacggttacg gggtcaagcg ggaagctttt catgaaaccg atcttgttcc
     4981 gggggagggg agtcgttctg cggaagaagt ttgaaagggt atcattaaca ccaggccgga
     5041 tcacatccgg cctggtgtcc caatgcctgc aatgaatgat agtcgcgtct tatcatgcca
     5101 ccattataaa attatcgctg ttttgcgg
//
LOCUS       DADLXX010000192         5116 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLXX010000192 DADLXX010000000
VERSION     DADLXX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.196"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..366)
                     /locus_tag="JNX27_003831"
     CDS             complement(<1..366)
                     /locus_tag="JNX27_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884847.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            892..1866
                     /gene="hemB"
                     /locus_tag="JNX27_003832"
     CDS             892..1866
                     /gene="hemB"
                     /locus_tag="JNX27_003832"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7884848.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNX27_003833"
                     /pseudo
     CDS             complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNX27_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNX27_003834"
     CDS             complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNX27_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7884849.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLLLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNX27_003835"
     CDS             complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNX27_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7884850.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNX27_003836"
     CDS             complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNX27_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7884851.1"
                     /translation="VPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTH
                     YSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTD
                     SEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENIS
                     KLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVAN
                     DYSQYVTSRFVQ"
BASE COUNT         1243 a         1408 c         1273 g         1192 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa
      541 ttgataaaag gacgatattt cagcatgttg cagataatgt tatttttgat gcatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc caaaaaaaat aataaccaag agctataaat
      721 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      781 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      841 ccccgcttca ggtatactgt gcaccttaat tccaaaaaca tcaggcagac catgacagac
      901 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      961 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
     1021 tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc
     1081 gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac
     1141 cataccgatg aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg
     1201 cgcatctgca agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa
     1261 tacacttctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
     1321 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
     1381 ccttccgccg cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc
     1441 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg
     1501 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
     1561 atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgc
     1621 ctgatggtta aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1681 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1741 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1801 gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg
     1861 cgttaatttt tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc
     1921 cctcacccta accctctccc caagggggcg agggaaccgt ccacactcgt atcaccccgc
     1981 ccgataaaac ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag
     2041 gtaatcggca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     2101 ttgccagcgc caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa
     2161 caaggcttcg ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac
     2221 aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg
     2281 atttttcgcg accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta
     2341 ttccgggaat gatttacgga aatcatgctc cgcacgcagc cagctcagca gctggcggaa
     2401 gggaacagag agcgcctcat aggccgcaat accgctggtt cagagcgtat caccgccggt
     2461 cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac
     2521 atcggtatgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat
     2581 ctcgtcaacc ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg
     2641 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgatg
     2701 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc
     2761 ggcacccgaa atttgtgcgc cgatatgcgg ccccagcggg gtaatgctca gacgttcact
     2821 cattgtactt ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct
     2881 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga
     2941 aattcacccg ctgactgaac cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc
     3001 gctaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     3061 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     3121 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccctg ccagcgccga catcgccacc
     3181 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag
     3241 atcaccatca gcagcaaata agccagcggt ggcaccggac gataaagttc gattaccgga
     3301 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     3361 accaccgccg cgagtagcgc ctgcacaatg cgcgtcaggc tggctgccag atgctgccac
     3421 agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt
     3481 tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca
     3541 gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     3601 catttcagcc gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3661 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3721 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3781 ccagcacacg gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt
     3841 cttctatatc gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca
     3901 gcagagtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcatcgagta
     3961 ataacagctg gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccat
     4021 cggaaagctg ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct
     4081 ggtgcgcgat ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca
     4141 cgttgtcctg tacattgcgc cacggtagca gcccttcatt ctgaaaaact acgccacgct
     4201 ctgctcccgg tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca
     4261 caaaaccggc aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca
     4321 ccagtagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     4381 aatcggcata gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     4441 aggtaacgta ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa
     4501 actgcgcggt gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct
     4561 gcggcgtcag ataggtattt cccttcacca gccccggaac gtcaccttca ggcacgccgc
     4621 ttaaacgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtca gggttagcaa
     4681 tgtacggttg ctgtgcatcg atggcgcttt tggcgaacgc tttcacgacc tcaggatgtt
     4741 tctcggcaaa atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga
     4801 cctgttcaga atcggtcagc accttgccgt ctttttccag ggcgttaacc gccggtgccc
     4861 agacataagc accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca
     4921 ggttcacaat ctccactagc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc
     4981 tgtagtgggt ggtggagata aacggtacgg caatgcgttt accaatcaaa tcttcgggtt
     5041 tgctgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     5101 agacttcaat cggcac
//
LOCUS       DADLXX010000193         5059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLXX010000193 DADLXX010000000
VERSION     DADLXX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.197"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            140..661
                     /locus_tag="JNX27_003837"
     CDS             140..661
                     /locus_tag="JNX27_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY7884852.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            717..950
                     /locus_tag="JNX27_003838"
     CDS             717..950
                     /locus_tag="JNX27_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY7884853.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1014..2972
                     /locus_tag="JNX27_003839"
     CDS             1014..2972
                     /locus_tag="JNX27_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7884854.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     SFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3027..3461
                     /gene="psiB"
                     /locus_tag="JNX27_003840"
     CDS             3027..3461
                     /gene="psiB"
                     /locus_tag="JNX27_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAY7884855.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3458..4177
                     /locus_tag="JNX27_003841"
     CDS             3458..4177
                     /locus_tag="JNX27_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAY7884856.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4457..4615
                     /locus_tag="JNX27_003842"
     CDS             4457..4615
                     /locus_tag="JNX27_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7884857.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT         1146 a         1327 c         1575 g         1011 t
ORIGIN      
        1 catgcggcga aggttctcaa acaggtgatg gtgaataccg gagggaacaa agagcaggtc
       61 cccgcaaaac gtcaaaaccg cggctcccga aactcaacag atgatggggg ctgaaaacat
      121 caacagaagg agacacatca tggcagttcg tggcattaac aaggtcattc ttgtcggtcg
      181 tctgggaaaa gatccggaag tccgttacat ccccaacggg ggcgcagtgg caaacctgca
      241 ggtggccacg tcagaaagct ggcgtgacaa acagacgggg gagatgcggg agcagacaga
      301 atggcatcgc gtggtgctgt tcggcaagct cgcggaagtg gcaggtgaat atctgcgcaa
      361 gggcgcgcag gtctacatcg aggggcaact ccgaacccgt agctgggaag ataaaggtat
      421 tacccgttac gtcactgaaa tccttgtgaa aaccacgggc accatgcaga tgctggggag
      481 tgcaccacag cagaacgctc aggcgcaacc gcagcctcag cagaatggtc agccacagag
      541 tgctgacacg acgaaaaaag ggagcgcgaa aacaaaaggc cgtggacgta aggccgcgca
      601 gccggagcct cagcagccgt ctgaacccgc gtatgacttc gatgacgaca ttccgttcta
      661 acgaaagaca gtgacaaccg ccccgtccag tgcggggcat caccggagat atgaggatga
      721 gcgaatattt cagaatattt caggggctgc ctgacggctc ctttacccgc gaacaggctg
      781 aagccgttgc cgcacagtac cggaatgtct ttatcgagga tgatcaggga acgcattttc
      841 gcctggttgt ccgcaataac agtggcctgg tctggcgtac ctggaatttt gaggacggtg
      901 ccgggtactg gatgaaccat gtcatccgga atttcggcat tcttaagtaa gagaggtgcc
      961 ggacgggaac gcgtccggca gacatacaac catcccggtc aaaggagaaa tatatgtcag
     1021 ttacagagtc taagacaaaa acggagcgta aatccagccg taaacctgca aaaacgcagg
     1081 aaacagtcct gtctgccctg ctggagcaga cggaggaagt gagcgtgtcg ctggcctcgc
     1141 tgattaaatc accgctgaat gtgcgcacgg tgccgtattc cgcagagtcc gtcagcgaac
     1201 tggcggagtc cattaagggc gttggcctgt tgcagaatct ggttgttcat gccctgcctg
     1261 gtgaccgcca cggtgtcgcc gcaggtggtc gccgactggc agcactcaac atgctggcag
     1321 agcgtggcat ccttccggct gactggcctg tacgcgtgaa ggttattccg caggagctgg
     1381 cgacggctgc atcaatgact gaaaacggtc agcgtcggga tatgcaccct gccgaacaga
     1441 ttgccggatt ccgcgcaatg gcgcaggaag gcaaaacacc tgcacaaatc ggtgatttgc
     1501 tgggctattc accccgccac gttcagcgaa tgctgaaact ggctgacctt gcgcctgtca
     1561 tcctcgatgc gctggcagaa gaccgcatca ccaccgaaca ctgtcaggcg ctggcgctgg
     1621 agaacgacac cgcgcgtcag gtgcaggtgt ttgaagccgc ctgtcagtcg ggatggggcg
     1681 gtaaaccgga agtacagacc attcgtcgtc tggtgaccga aagtgaagtg gcggtggcgg
     1741 ggaacagtaa attccgcttc gtgggggctg atgccttctc gccagacgaa ctgcgcaccg
     1801 atttgttcag cgacgacgaa ggtggctatg tggactgcgt ggcgctcgat gccgccctgc
     1861 tggaaaaact ccaggctgtc gcagagttcc ttcgggaagc cgaaggctgg gaatggtgcg
     1921 ccggacgcat ggagcctgtc ggtgagtgcc gtgaggatgc cggaacatac cgctgtctgc
     1981 cggagccgga agcggtgctg acggaggcgg aagaagaacg tctgaacgaa ctgatgacgc
     2041 gttacgacgc gctggaaaac cagtgtgagg aatctgacct gctggaagca gaaatgaagc
     2101 tgatgcgctg catggcgaag gtcagggcgt ggacgccgga gatgcgtgcc ggaggtggag
     2161 tggtggtgtc ctggcgttat ggcaacgtgt gtgtccagcg tggtgtgcag ttgcgcagtg
     2221 aggatgacgt ggctgacgac gctgaccgta cggaacaggt gcaggagaaa gcgtcagtgg
     2281 aggaaatcag tctgccactg ctgacgaaaa tgtcttccga acgcacgctg gcagtccagg
     2341 cagcactgat gcagcaaccg gacaaatccc tgacactgct ggcatggacg ctctgcctga
     2401 atgtgtttgg cagcggagcg tacagtaaac cagcacaaat cagcctggaa tgtaaacatt
     2461 attcgctgac cagcgatgcg ccatcgggga aggaaggtgc cgcattcatg gcgctgatgg
     2521 cagaaaaagc ccgtcttgct gccctgttac cggagggatg gtcacgggac atgacgacat
     2581 tcctgtcact cagtcaggag gtgctgttat ccctgctcag tttctgcacc gcatgcagcc
     2641 ttaacggtgt ccagacccgt gagtgtggtc acacgtcacg cagtccgctt gacacgctgg
     2701 aaagcgccat cagctttcac atgcgcgact ggtggcagcc gacaaaagca aacttcttcg
     2761 gacacctgaa aaagccgcag attatcgcag ccctgaatga ggcagggctg tccggtgccg
     2821 cacgggacgc ggagaagatg aagaaaggtg atgcggctga acatgcagag caccatatga
     2881 aagacaaccg ctgggtgcct ggctggatgt gtgcaccaca tccacagaca gataccactg
     2941 aacgcaccga taacctggct gatgccgcct gatgaacaac cacaccgccc cgccggagac
     3001 ggggcggcag caagggagat accgtgatga aaactgaact gaccctgaat gtattacaga
     3061 ccatgagcgc acaggaatat gaagatatcc gggctgccgg aagtgatgaa cgtcgtgagc
     3121 tgacacacgc cgtgatgcgg gaactggatg cgccggacaa ctggacgatg aacggcgagt
     3181 acggcagcga gtttggcgga tttttccccg tccaggtccg tttcacgcct gcccacgaac
     3241 gttttcacct ggcgttatgt tcaccgggcg atgtctctca ggtctgggtg ctggttctgg
     3301 tgaatgccgg tggtgagccg tttgcggtgg ttcaggtaca gcggcggttt gcaccggaag
     3361 ccgtcagcca ttcactggca ctggcggcgt cactggacgc gcaggggtac agcgtcagtg
     3421 acatcatcca tatcctgatg gcggaaggag gtcaggcatg agcgcacgtt cacaggcact
     3481 ggttcccctc agcgcagagc aacaggccgc atggcgggcg gtggcggaga cagaaaaacg
     3541 ccgccaccag ggaaacacgc tggcggaata tccgtatgcg ggggcgtttt tccgctgtct
     3601 gaacggaagc cgcaggatat cgctgtctga cctgcgattt ttcatgccgt ccctgaccgc
     3661 agaagaactg cacggcagcc gtcttcagtg gctgtatgcc atcgatgtgc tgattgaaac
     3721 acagggcgag gtctgcctcc ttcctcttcc aggcgatgca gcagaacgct tgttcccgtc
     3781 ggtgcggttt cgtgtcaggg aacgcagtcg ccataaatcc gcgctggtca tgcagaagta
     3841 cagccgccag caggcgaggg aggcagagca gaaagcccgt gcgtaccagg cgctggtcgc
     3901 tcaggctgaa atcgaactgg cattccattc gccggaaaca gtcggaagct ggtatgcacg
     3961 ctggagtgac cgggtggcag agcatgacct ggaaaccctg ttctggcagt ggggtgagcg
     4021 ttttccgtca ctggccggaa tggaacgctg gcagtggcag gatatgccgt tctggcaggt
     4081 tattgcggaa gccagtcttg cagcaagaga ggccggtcac gctgtccgtg agatggagcg
     4141 gtggatggtg ccgaacaaac tccgggaggc ggcgtgatgc ggcaacaatc acacagatta
     4201 cccgtaaaca gcctgaatga gcgggttatt ttcaggaaaa gtgagtgtgg tcagcgtgca
     4261 gggatatggg ctatgatgtg cccggcgctt gaggctttct gcctcatgac gtgaaggtgg
     4321 tttgttaccg tgttgtgtgg cagaaagaag atagccccgt agtaagttaa ttttcattaa
     4381 ccaccacgag gcatccctat gtctagtcca catcaggata gcctcttacc gcgctttgcg
     4441 caaggagaag aaggccatga aactaccacg aagttccctt gtctggtgtg tgttgatcgt
     4501 gtgtctcaca ctgttgatat tcacttatct gacacgaaaa tcgctgtgcg agattcgtta
     4561 cagagacgga cacagggagg tggcggcttt catggcttac gaatccggta agtagcaacc
     4621 tggaggcggg cgcaggcccg ccttttcagg gctgatgctg gtctgactgc actgaagcgc
     4681 ctttataaag gggctgctgg ttcgccggtg gcccctttct ccttgctggc attgtacggg
     4741 catgaacaac cagactgcca ggaaggccgt ttccctccgg gaacggggat gaacgtgcgc
     4801 ctgcggcgcc cgttcttttc ccccgccttc tctgtttatg acttaccaga aatcacatca
     4861 ccttcccttt gattattctc tttttcctcc tgctctgact ctgattactg ctctgactct
     4921 gattactgct gccctgatta ttgtctgttt tgttgttctc ctgcttatgc cttttccggg
     4981 cgtccccctg gcggggcggg ctgtcgtgaa cggagccagc gaagctgtct ccgccctggc
     5041 gggcttccat ccctgacgc
//
LOCUS       DADLXX010000194         4992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLXX010000194 DADLXX010000000
VERSION     DADLXX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.198"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="JNX27_003843"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="JNX27_003843"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7884858.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNX27_003844"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNX27_003844"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7884859.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="JNX27_003845"
     CDS             2152..2610
                     /locus_tag="JNX27_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7884860.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="JNX27_003846"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="JNX27_003846"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7884861.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="JNX27_003847"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="JNX27_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7884862.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="JNX27_003848"
     CDS             3816..4493
                     /locus_tag="JNX27_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7884863.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="JNX27_003849"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="JNX27_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7884864.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1213 a         1186 c         1315 g         1278 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac cctaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga atgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatc agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg atttcaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tc
//
LOCUS       DADLXX010000195         4982 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLXX010000195 DADLXX010000000
VERSION     DADLXX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.199"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            255..1199
                     /gene="leuO"
                     /locus_tag="JNX27_003850"
     CDS             255..1199
                     /gene="leuO"
                     /locus_tag="JNX27_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7884865.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            1517..3241
                     /gene="ilvI"
                     /locus_tag="JNX27_003851"
     CDS             1517..3241
                     /gene="ilvI"
                     /locus_tag="JNX27_003851"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7884866.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3244..3735
                     /gene="ilvN"
                     /locus_tag="JNX27_003852"
     CDS             3244..3735
                     /gene="ilvN"
                     /locus_tag="JNX27_003852"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7884867.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3747..3833
                     /locus_tag="JNX27_003853"
     CDS             3747..3833
                     /locus_tag="JNX27_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7884868.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3915..4919
                     /gene="cra"
                     /locus_tag="JNX27_003854"
     CDS             3915..4919
                     /gene="cra"
                     /locus_tag="JNX27_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7884869.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1210 a         1133 c         1315 g         1324 t
ORIGIN      
        1 gatgattgag tattcgcggt agttatgatt agattgtttt cgcaacaaaa acattatgga
       61 ttattatgct gtggtaaatg actcattcca cggcaatgga ttctgttttt atcagaaccc
      121 gtatctttat gttttccgaa ttttactcat tttgcttttt cttattttat atgcatgata
      181 aatcatattc ttcaggatta tttctctgca ttccaataag ggaaagggag ttaagtgtga
      241 cagtggagtt aagtatgcca gaggtacaaa cagatcattc agagacggcg gagttaagca
      301 agccacagct acgcatggtc gatctcaact tattaaccgt tttcgatgcc gtgatgcagg
      361 agcaaaacat tacccgtgcc gctcatgttc tgggtatgtc gcaacctgcg gtcagtaacg
      421 ctgttgcacg cctgaaggtg atgtttaatg acgagctttt tgttcgttat ggccgtggta
      481 ttcaaccgac tgctcgcgca tttcaacttt ttggttcagt tcgccaggca ttgcaactag
      541 tacaaaatga attgcctggt tcaggttttg aacccgcgag cagtgaacgt gtatttcatc
      601 tttgtgtttg cagcccgtta gacagcattc tgacctcgca gatttataat cacattgagc
      661 agattgcgcc aaatatacat gttatgttca agtcttcatt aaatcagaac actgaacatc
      721 agctgcgtta tcaggaaacg gagtttgtga ttagttatga agacttccat cgtcctgaat
      781 ttaccagcgt gccattattt aaagatgaaa tggtgctggt agccagcaaa aatcatccaa
      841 caattaaagg cccgttactg aaacatgatg tttataacga acaacatgcg gcggtttcgc
      901 tcgatcgttt cgcgtcattt agtcaacctt ggtatgacac ggtagataag caagccagta
      961 tcgcgtatca gggcatggca atgatgagcg tacttagcgt ggtgtcgcaa acgcatttgg
     1021 tcgctattgc gccgcgttgg ctggctgaag agttcgctga atccttagaa ttacaggtat
     1081 taccgctgcc gttaaaacaa aacagcagaa cctgttatct ctcttggcat gaagctgccg
     1141 ggcgcgataa aggccatcag tggatggaag aacaattagt ctcaatttgc aaacgctaac
     1201 tgaatgcaga ataggccaga catgaatgtc tggtttattc tgcatttttt attgaatgta
     1261 gaattttatt ctgaatgtgt ggactctcta ttttaggatt aattaaaaaa atagagaaat
     1321 tgctgtaagt tgtgggattc agccgattta ttatcaattt aatccttcgt aatggaggat
     1381 tttatcgttt cttttcacct ttcctcctgt ttattcttat taccctgcat ttatgtctct
     1441 ggctgccaat tgcttaagca agatcggacg gttaatgtgt tttacacatt ttttccgtca
     1501 aacagtgagg caggccatgg agatgttgtc tggagccgag atggtcgtcc gatcgcttat
     1561 cgatcagggc gttaaacaag tattcggtta tcccggaggc gcagtccttg atatttacga
     1621 tgcattgcat accgtgggtg gtattgatca tgtattagtt cgtcatgagc aggcggcggt
     1681 gcatatggcc gatggcctgg cgcgtgcgac cggggaagtc ggcgtcgtgc tggtaacgtc
     1741 gggtccaggg gcgaccaatg cgattaccgg catcgccacc gcttatatgg attccattcc
     1801 attagttgtc ctttccgggc aggtagcgac ctcgttgata ggttacgatg cttttcagga
     1861 gtgcgacatg gtggggattt cgcgaccggt ggttaaacac agttttctgg ttaagcaaac
     1921 ggaagacatt ccgcaggtgc tgaaaaaggc tttctggctg gcggcaagtg gtcgcccagg
     1981 accagtggtc gttgatttac cgaaagatat tcttaatccg gcgaacaaat taccctatgt
     2041 ctgccctgag tcggtcagta tgcgttctta caatcccact actaccggac ataaagggca
     2101 aattaagcgt gctctgcaaa cgctggtagc ggcaaaaaaa ccggttgtct acgtaggcgg
     2161 tggggcaatc atggcgggct gccatcagca gttgaaagaa acggtggagg cgttgaatct
     2221 gcccgttgtt tcctcattga tggggctggg ggcgtttccg gcaacgcatc gtcaggcact
     2281 gggcatgctg ggaatgcacg gtacctacga agccaatatg acgatgcata acgcggatgt
     2341 gattttcgcc gtcggggtac gatttgatga ccgaacgacg aacaatctgg caaagtactg
     2401 cccaaatgcc actgttctgc atatcgatat tgatcctact tccatttcta aaaccgtgac
     2461 tgcggatatc ccgattgtgg gggatgctcg ccaggtcctc gaacaaatgc ttgaactcct
     2521 gtcgcaagaa tccgcccatc aaccgctgga tgagatccgc gactggtggc agcagattga
     2581 acagtggcgc gctcttcagt gcctgaaata tgacactcac agtgaaaaga ttaaaccgca
     2641 ggcggtgatc gagactcttt ggcggttgac taatggggac gcttacgtga cgtccgatgt
     2701 cgggcagcac cagatgtttg ctgcgcttta ttatccattc gacaaaccgc gtcgctggat
     2761 caattccggc ggtctcggca cgatgggttt tggtttacct gcggcactgg gcgtcaaaat
     2821 ggcgttgcca gaagaaaccg tggtttgcgt cactggcgac ggcagtattc agatgaacat
     2881 ccaggaactg tctaccgcgt tgcaatacga gttgcccgta ctggtggtga atctcaataa
     2941 ccgctatctg gggatggtga aacagtggca ggacatgatc tattccggcc gtcattcaca
     3001 atcttatatg caatcactac ccgatttcgt ccgtctggcg gaagcctatg gtcacgtcgg
     3061 gatccagatc tctcatccgc atgagctgga aagcaaactt agcgaggcgc tggaacaggt
     3121 gcgcaataat cgcctggtgt ttgttgatgt taccgtcgat ggcagtgagc acgtctaccc
     3181 gatgcagatt cgcgggggcg gaatggatga aatgtggtta agcaaaacgg agagaacctg
     3241 attatgcgcc ggatattatc agtcttactc gaaaatgaat caggcgcgtt atcccgcgtg
     3301 attggccttt tttcccagcg aggctacaac attgaaagcc tgaccgttgc gccaaccgac
     3361 gatccgacat tatcgcgtat gaccatccag accgtgggcg atgaaaaagt acttgagcag
     3421 atcgaaaagc aattacacaa gctggtcgat gtcttgcgcg tgagtgagtt ggggcagggc
     3481 gcgcatgttg agcgggaaat catgctggtg aaaattcagg ccagcggtta cgggcgtgac
     3541 gaagtgaaac gtaatacgga aatattccgt gggcaaatta tcgatgtcac cccctcgctt
     3601 tataccgttc aattagcagg caccagcgat aagcttgatg catttttagc atcgattcgc
     3661 gatgtggcga aaattgtgga agttgctcgc tctggtgtgg tcggactttc gcgcggcgat
     3721 aaaataatgc gttgagaatg atctcagtgc gcaatttgca gcccaacatg tcacgttggg
     3781 ctttttttgc gaaatcagtg ggaacctgga ataaaagcag ttgccgcagt taattttctg
     3841 cgcttagatg ttaatgaatt taacccatac cagtacaatg gctatggttt ttacatttta
     3901 cgcaaggggc aattgtgaaa ctggatgaaa tcgctcggct ggcgggagtg tcgcggacca
     3961 ctgcaagcta tgttattaac ggcaaagcga agcaataccg tgtgagcgac aaaaccgttg
     4021 aaaaagtcat ggctgtggtg cgtgagcaca attaccaccc gaacgccgtg gcagctgggc
     4081 ttcgtgctgg acgcacacgt tctattggtc ttgtgatccc cgatctggag aacaccagct
     4141 atacccgcat cgctaactat cttgaacgcc aggcgcggca acggggttat caactgctga
     4201 ttgcctgctc agaagatcag ccagacaacg aaatgcgctg cattgagcac cttttacagc
     4261 gtcaggttga tgccattatt gtttcgacgt cgttgccacc tgagcatcct ttttatcaac
     4321 gctgggctaa cgacccgttc ccgattgtcg cgctggaccg cgccctcgat cgtgaacatt
     4381 tcaccagcgt ggttggtgcc gatcaggatg atgccgaaat gctggcggaa gagttacgta
     4441 agtttcccgc cgagacggtg ctttatcttg gcgcgctgcc ggagctttct gtcagcttcc
     4501 tgcgtgaaca aggtttccgt actgcttgga aagatgatcc gcgcgaagtg catttcctgt
     4561 atgccaacag ctatgagcgg gaggcggctg cccagttatt cgaaaaatgg ctggaaacgc
     4621 atccgatgcc gcaggcgctg ttcacaacgt cgtttgcgtt gttgcaagga gtgatggatg
     4681 tcacgttgcg tcgcgacggc aaactgcctt ctgacctggc aattgccacc tttggcgata
     4741 acgaactgct cgacttctta cagtgtccgg tgctggcagt ggctcaacgt caccgcgatg
     4801 tcgcagagcg tgtgctggag attgtcctgg caagcctgga cgaaccgcgt aagccaaaac
     4861 ctggtttaac gcgcattaaa cgtaatctct atcgccgcgg cgtgctcagc cgtagctaag
     4921 ccgcgaacaa aaatacgcgc caggtgaatt tccctctggc gcgtagagta cgggactgga
     4981 ca
//
LOCUS       DADLXX010000196         4951 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLXX010000196 DADLXX010000000
VERSION     DADLXX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.200"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..611
                     /locus_tag="JNX27_003855"
                     /pseudo
     CDS             1..611
                     /locus_tag="JNX27_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
     gene            632..1292
                     /gene="csiR"
                     /locus_tag="JNX27_003856"
                     /pseudo
     CDS             632..1292
                     /gene="csiR"
                     /locus_tag="JNX27_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNX27_003857"
     CDS             complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNX27_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7884870.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1826..1984)
                     /locus_tag="JNX27_003858"
     CDS             complement(1826..1984)
                     /locus_tag="JNX27_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7884871.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2167..2466
                     /locus_tag="JNX27_003859"
     CDS             2167..2466
                     /locus_tag="JNX27_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7884872.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2476..3000
                     /gene="ygaP"
                     /locus_tag="JNX27_003860"
     CDS             2476..3000
                     /gene="ygaP"
                     /locus_tag="JNX27_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7884873.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNX27_003861"
     CDS             complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNX27_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7884874.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4119..4556
                     /gene="alaE"
                     /locus_tag="JNX27_003862"
     CDS             4119..4556
                     /gene="alaE"
                     /locus_tag="JNX27_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7884875.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4593..4943)
                     /locus_tag="JNX27_003863"
     CDS             complement(4593..4943)
                     /locus_tag="JNX27_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7884876.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
BASE COUNT         1261 a         1167 c         1165 g         1358 t
ORIGIN      
        1 tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc aatattcccc
       61 atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc ctgaactcgg
      121 ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat gctcccgcgg
      181 taatgggtaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc tccaccggcg
      241 cagcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt aaatttctga
      301 tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
      361 ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg tggctttatc
      421 cctggctcac ctagctggtc atcggcttta ttacctttgt gttggtagtg atgctattcc
      481 gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg attatctgta
      541 ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata
      601 atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg
      661 ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag
      721 tttactgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagtac tttctcaact
      781 ggtggtggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc
      841 agagcaggag ctgctctgac atagacgccc gcgccaatat ggaagcgatg ctggtcagtc
      901 tggcgattgc ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc
      961 tcaataagct ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     1021 atcaggcgtt tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     1081 gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc
     1141 tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     1201 tggcgcgaga tacagcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     1261 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc
     1321 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     1381 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     1441 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg
     1501 gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg
     1561 ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca
     1621 ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc
     1681 tgacctgtaa ccgcatccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     1741 atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt
     1801 taacgccgcg ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     1861 aaccggggat atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc
     1921 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     1981 ccatatgtac tccctataag aaaattactc attgtttaaa aagagacttt atctcttaag
     2041 cgtagtaatt ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg
     2101 ctgaggctgg tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa
     2161 ataaatatga ctgaactcgc gcaattacag gccagtaccg aacaggcagc ggccttattg
     2221 aaagcaatga gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc
     2281 accagcgcgg gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat
     2341 ctcgctcgta tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta
     2401 tattccatta aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt
     2461 ccgtaaggag tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc
     2521 gcacgcggcg caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt
     2581 cctgaagcag atctggctcc attatccgtg ctggaacagt caggtcttcc acctaaatta
     2641 cgtcgcgagc aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat
     2701 aaattagcag cgattgccgc ccccgcagaa atatttttac tcgaagatgg gattgatggc
     2761 tggaaaagag ctggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc
     2821 caggtgcaga tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta
     2881 aatagcggtt tcttcttatt aagtggcttt gttggtgccg ggttactgtt tgcaggaatc
     2941 agcggttttt gtggaatggc aaggttgtta gataagatac cgtggaacca acgagcttga
     3001 gaagcgacgc cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat
     3061 cgtcgagaga tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac
     3121 cggtccaggt tttagtttca ccgttaacat cagtgaattt atatttcgcc ggacgcggct
     3181 ggcgtttttt accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt
     3241 taattccgtc agctttcatc agctccagcc aggtgctaat tttttcctga cgctcagcca
     3301 gttcacgctg ctgctgttct tcttcttcac gcctttcttt agtgacaacc ctgaattttt
     3361 cgagcatttc ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa
     3421 tgttatttaa actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca
     3481 aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc
     3541 tatttacagt cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt
     3601 tcaagattat atattagcca ttttgatgag taatttaggt tattggtgaa cagagtggcg
     3661 ctctctgctc gctaactccg taagcctgac aaaaccgaag tgaataaaaa tgcaatataa
     3721 cagattaaaa gaaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca
     3781 tcaaaaatgc tgtaaatgac ccggtcacga caaacaccga attctctacg cttatagcgt
     3841 tacctccccc ccaaactttc attagtgaaa ttaaaggcaa ggccatatcc aattgattta
     3901 attgaaaata aatcatatcg acatgtaaaa atccacaatg tacaaaaaac gagctgttac
     3961 ggaatatttt atctacaaaa actgactaaa taaaaatttt tcactaattg attagtaata
     4021 gccagcgata tacgctatgc gaaaatacag atggcaatga gatccactgc tttcatctcc
     4081 attaacatcc cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct
     4141 tgcgtcatgc agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt
     4201 gtattgaagt tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag
     4261 cgattccggt gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc
     4321 gcgcggcacg caaaggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc
     4381 agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg
     4441 cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc
     4501 tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct
     4561 tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc
     4621 aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt
     4681 cgctgtcgct cgcctgctaa atagagcgga aattgctggt aatgatcgca agttttaata
     4741 tcggaagcgg gttcagccag tgttgaactg cgttctttta atgttggatg aattaccaga
     4801 gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa
     4861 ccatacgctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc
     4921 acctcgtctg gtcgtaaata catatttgat c
//
LOCUS       DADLXX010000197         4824 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLXX010000197 DADLXX010000000
VERSION     DADLXX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.201"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            447..1202
                     /locus_tag="JNX27_003864"
     CDS             447..1202
                     /locus_tag="JNX27_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY7884877.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            1281..1354
                     /locus_tag="JNX27_003865"
     tRNA            1281..1354
                     /locus_tag="JNX27_003865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1313..1315,aa:Gly,seq:ccc)
     gene            1489..3096
                     /locus_tag="JNX27_003866"
     CDS             1489..3096
                     /locus_tag="JNX27_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7884878.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPDPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVSKATRADMLRVREVLQLLPKNCKQRFKAMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTVLAAITGARLNEVAQLQVKDVRTTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTMGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVNG"
     gene            3089..3742
                     /locus_tag="JNX27_003867"
     CDS             3089..3742
                     /locus_tag="JNX27_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAY7884879.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            4683..>4824
                     /locus_tag="JNX27_003868"
     CDS             4683..>4824
                     /locus_tag="JNX27_003868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884880.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEP"
BASE COUNT         1278 a         1149 c         1211 g         1186 t
ORIGIN      
        1 tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg aacatttggt
       61 tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga cgataagcgt
      121 taggggagga aggaagtgtg atgacgttaa aaaaacacag atatgatacg aaacaggact
      181 ctgaataggg acagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaatca
      241 ggattattac gcatcaggat gggaaatagg gtaaaaagtc aggaacttcg atacctctac
      301 agcgaatatt ttgctgatga tacttccctt tgccaaatga aattaacctc tttctctatt
      361 accacgtttt tccagaagca agagattggg tatcaagagg ctggctgcta tgatatggcg
      421 tcttgttttt taagcgagga aagattttga gtgcgggacg cctgaataaa aaatctctgg
      481 gtatcgtgat gtttttatcg gttggactgc tattggcggg ctgttcgggt agcaaatcat
      541 ccgatacagg aacgtattcc ggctccgttt acaccgtgaa acggggggat acgctatatc
      601 gtatttcgcg caccacggga accagcgtaa aagagctggc gcgactgaac ggcatttccc
      661 ccccttacac cattgaagtt ggtcagaaac taaaactggg tggggcgaaa agtagcagta
      721 gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag
      781 cggtaccgaa atcttcctgg ccgccagtag ggcaacgttg ttggttatgg ccaacgacag
      841 ggaaagttat catgccgtat tcgacagcag atggcggcaa taaagggatt gatatctcag
      901 ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc
      961 agctgcgtgg ctacggtaat ctcatcatga ttaaacacag tgaagattac attacggctt
     1021 acgcccataa tgacacgatg ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa
     1081 tcgccactat ggggagcacg gatgcggcat ctgttcgcct gcatttccag attcgttacc
     1141 gtgcaacggc aattgatccg ctacgttact tgccgccgca gggcagcaag ccaaaatgct
     1201 gatggcgaat taatcagcag tcagcagcgc ggcacttgct aaggagagcg taaggtttat
     1261 aatgccttac gcatctcgaa gcgggcgtag ttcaatggta gaacgagagc ttcccaagct
     1321 ctatacgagg gttcgattcc cttcgcccgc tccatgacat ttcttaaagt tcaataactt
     1381 actgaatcct aagagatatg gcttaacgcc ttccgatagt tttactcact gtaaccaaca
     1441 ctgtgactaa ctttgttgaa gtagtgagca gacctaaggt ctcagtacat gctcaactca
     1501 agaacctacc tttatcagcg taatggtgtc ttttacatcc gtcttaggat gaagaccact
     1561 ggtcgcctta ccgcttcgct tccttctcac aaccgttata aactagcgtc agtatcttta
     1621 cggaccaagg atagacgtac cgctatggct cactcccggc acatcaagtc agcacttaaa
     1681 gcaatccatg cagacaaccc taacgcctct tatgaggagc tacgggagca cctgaagacc
     1741 attgtcgagt gggaactcag tgtcagccgc gatgacctga acgaccctga gtcctatcag
     1801 ctttacgttg accagtacga cgatattaag tccaaccttc gggaagctgt agcgactgag
     1861 cacctgactg tagaccagca tcgctatatc aatgacgtta tcggtgtgct taaagcctgt
     1921 caggatagac tcaaaggtga tagctctggt ctgttgtctt accttgagcc tgagactggc
     1981 agtctaagac cgtccgtctc cctatctgta ttggcagaac ctgaagtccc tgaccctaaa
     2041 gctctgaccc tcgcgtctct cattgagcag tacgagcagg agaacgccca gaactggaag
     2101 ccagcgaccc tgagcgagaa ccgagcgtcg cactccacgc taatcgagat attcgactat
     2161 ctggatattc aggacgtcag caaggcgacc cgtgcagata tgcttagggt ccgtgaagtc
     2221 ctccaactgt taccgaagaa ctgcaaacaa cgctttaagg ctatgccgct gtctgacctg
     2281 ctgaaccggg agtctaagac tgactgtctg gatgtcgtta ccatcaacaa taaatacctt
     2341 atcaagatgg ctgctgtgtt taagtgggcg gtacgcaacg acctgatagc taagaacctg
     2401 acagaaggtc ttgagctgaa agtcccgcag cgtaaagcct ccgacgctcg tgatgcgttc
     2461 agtcctgagc aggtagggca actactggtc gcagctaaag cgtactcgca gaagacctca
     2521 gggaagccat accattatta cgtcaccgtt ctggctgcaa tcactggagc gagacttaac
     2581 gaggtggccc aacttcaggt caaagacgtc aggaccactg aggccgggac cgtgtatatc
     2641 catatcaacg aagacgatag cagcttgccg ggtaagagca tcaagaacgc ccacagtgac
     2701 cgctgtgtcc cgctggtcga tggcgcttat ggttttgtat tggctgactt tatgtcgctt
     2761 gtggaagacc gtagaaagac tatgggtgat aatgctatgg tattcgatgg cctcaagctg
     2821 atgaagaacg gctatggtga gcaggtgagc aaatggttta accgaacgtt gctacctaaa
     2881 gtccttgctg accgtagcgg cttagcgttt cactcgttta gacatacagt tgcgacccag
     2941 ctaaaacaac atggggtcga attggcgtat gctcaggcga ttatgggtca tagctctggg
     3001 tctataactt acgaccgtta cgcgaaagag gtcgaggtag atagattggt taatgtgatg
     3061 gcgggtgttt ataaggagac tggagtaaat ggctgagtat tatccggcag agtttggtgg
     3121 cactgctttt aactgcccga attgtggcgc ttattctcaa cagcgctggg tccgcttaaa
     3181 atatggcgct aacgggattt atgagacacc tttcgccttt tctaaatgtg aacgatgcaa
     3241 tgagaaagct tattggtatg acgaaaccat gattatacca gcgtcagcaa acgttgagat
     3301 gcctaaccca gacatgccag acgactgtaa gtccgactac atggaggcca gaagcattgt
     3361 taacctttct cctaaaggcg ctgccgcact cctgcgctta agtcttcaga agctgatggt
     3421 ccatctcggt gagccgggga agcatatcga cacggacatt aagtcgcttg tagccaaagg
     3481 attgtctcca ttggtccagc gagctgctga tatttgtcgt atcgttggca atcaggcggt
     3541 ccatccgggt gaaattaata ttgatgacga ccctcagtta gcgcatggtc tttttaagct
     3601 gctgaatatt attgtcactg agcagattac cagaccgaaa gaagtcgaag ccatgtttaa
     3661 cagcatgccg gaaagggcgc tcaagggcat tgaggaccgg gacaggaagg cgagggaaca
     3721 gcagcaggca gctagcgagt aaagctttag gtcagattga atataccctc actataggga
     3781 agggggctta actatcacta taggggagag accctaagtc cccaccttaa tagcagtcac
     3841 gccccatacg ctcattcatt ttagtctcag gcttccggta gcggaccgat tggaaaccat
     3901 ccgcagacgc ctagtgttaa atcaccttgg cctgagacca ttctccttta tcgtagcaat
     3961 ctctctcctt gacaggcgga gaacatcaga ccgcagcgga agtatccgtc tatgcgacca
     4021 tcggtgtgcg agattgtgaa gaaccaagac atcattgctg aggtctgcca tccattgtgg
     4081 acaggcggaa actcaaagag gttagaccag tagtggtgtc tgaggtttgg gctttgagtc
     4141 ctgaccagca ggctggagga gaccatgagt ttatcttgtt atggattgtg ttatcacaat
     4201 tccaataaga agaactttaa gtccagcctc cagtcctgtc agcccgaagt aaactatggt
     4261 cgctatgatg tggtctccat acttcgggtc taactggagg tcagattatg gccttatcag
     4321 gtagcaaatc ttacaccctc actgtagaga ccatacattc cgcctctggt cttgatgcgt
     4381 caagttatgc ggaagctctc ccgacattaa gtctttctcc gaagacatcc caaggcaaga
     4441 cagcctctgt cagacctcca gcccctagaa gttcttctcc tcttgaagtc tatcagcttc
     4501 tagcatctgg tcgtcctgac acgctgtcga accttcatat ctatggtcat cgcagaccac
     4561 atcacaatta cctcaagtct ttctgtatgt ctcctgttgc ctgtgagtct ccctatcgtt
     4621 gggggcttga gtaggctctc agggtgtgac ttcaggaggc ataacaaaag gagtacaacg
     4681 atatgtcaac tcaaccctta atgaagcatg aagtagttct tcctgtcatc gttgacggct
     4741 atcactgggc ctgtaatcag gaccgtatgt ggaacctgaa cgagctacat aaagagcttg
     4801 agcttccatc tgctaaggag ccgg
//
LOCUS       DADLXX010000198         4791 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLXX010000198 DADLXX010000000
VERSION     DADLXX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.202"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..531)
                     /gene="coaBC"
                     /locus_tag="JNX27_003869"
     CDS             complement(<1..531)
                     /gene="coaBC"
                     /locus_tag="JNX27_003869"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7884881.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAV"
     gene            703..1371
                     /locus_tag="JNX27_003870"
                     /pseudo
     CDS             703..1371
                     /locus_tag="JNX27_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            1588..1824
                     /gene="rpmB"
                     /locus_tag="JNX27_003871"
     CDS             1588..1824
                     /gene="rpmB"
                     /locus_tag="JNX27_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7884882.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1845..2012
                     /gene="rpmG"
                     /locus_tag="JNX27_003872"
     CDS             1845..2012
                     /gene="rpmG"
                     /locus_tag="JNX27_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7884883.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            2110..2919
                     /gene="mutM"
                     /locus_tag="JNX27_003873"
     CDS             2110..2919
                     /gene="mutM"
                     /locus_tag="JNX27_003873"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7884884.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2958..3437)
                     /gene="coaD"
                     /locus_tag="JNX27_003874"
     CDS             complement(2958..3437)
                     /gene="coaD"
                     /locus_tag="JNX27_003874"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7884885.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(3445..4722)
                     /gene="waaA"
                     /locus_tag="JNX27_003875"
     CDS             complement(3445..4722)
                     /gene="waaA"
                     /locus_tag="JNX27_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7884886.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
BASE COUNT         1188 a         1186 c         1298 g         1119 t
ORIGIN      
        1 caccgccata tccacaatgg ttaacggatc gagcattcgt ccaggaccga tatcaccaca
       61 agcctgactg ccactgtctg gcccccagat gagcaaacca cgggaagcaa gcacctctaa
      121 attatgctgc gtggcagcgg cacggtacat ctgctggttc atggcgggga gcacggctac
      181 aggcgcaggt gtagccagac aaatcgtcga taccaggtca ttcgccattc cggcagcaac
      241 acgggcaatc aaatctgccg tggcaggggc gagaatcact aaatcagccc atttacccag
      301 ctcaatatgg cccatagcgg cttcggctgc cgggtcaagc agactgtcgg aaacgggata
      361 accagaaacc gcctgcaagc taagtggggt gataaaggct tttgccgctt cggtcatggc
      421 tacgcggacg tcggccccgc gatcgcgcaa acgacgcacc agttcagggg ttttataggc
      481 agcaataccg ccgctaacgc cgagaacgat ttttttaccg gccaggctca tgatgatttt
      541 tcctgttggg tgacaccaga agttggcgat tttatcacaa tacttttgtt gtcgtgcctt
      601 cacctgagat tcactttgcg aggctctttc caggattgaa aactggccgt cgatttaacg
      661 gaacggctat gacaggatgc gagcaccaca aaggaggtga aggtgaaaaa caatgcacag
      721 ctgttgatgc cgcgcgaaaa aatgctgaag tttggtatta gcgccttaac ggatgtcgag
      781 ctgctggcgc tatttctgcg taccggaacg cgcggtaaag atgtattaac cctggcaaaa
      841 gagatgctgg agaatttcgg ctctctttat ggcttgttaa cctctgaata tgaacaattt
      901 agtggtgttc atggaattgg cgtagcgaaa tttgcccagt taaaggggat tgctgaactg
      961 gcgcgacgtt actacaacgt gcggatgcgt gaagaaagcc ctttactcag cccggagatg
     1021 acgcgggaat ttttacaaag ccagctcacg ggtgaggagc gggagatctt tatggtgatc
     1081 tttctcgact cacaacaccg ggttataacg catagccgtc ttttttccgg cacgctaaac
     1141 catgttgaag tccatcctcg ggaaattatc cgctaagcga taaaaataaa cgcctcggcg
     1201 ctgatccttg cacataatca cccttcgggt tgtgctgaac ccagtaaagc ggataaactc
     1261 attactgaac ggataataaa gagttgtcag ttcatggatt tacgcgtgct cgaccatatc
     1321 gtgattgggc gtggagagta tgtttctttt gccgaacgcg gctggattta acccgctatg
     1381 cgcgatcctt cgggatcttt gtctgttcgg gacttgagca catcgctgag tcagcgtata
     1441 ctacgccacc tttgagaatc tcgggtttgg catttgggcc tggcaatcga gagttcacat
     1501 agaactgcga tgaccgggct gtaaagcctg acgaggcgcc aataccccat acgaagctcg
     1561 agctaatttg atttttggag aatagacatg tcccgagtct gccaagttac tggcaagcgt
     1621 ccggtgaccg gtaacaaccg ttcccacgca ctgaacgcga ctaaacgccg tttcctgccg
     1681 aacctgcact ctcaccgttt ctgggttgag agcgagaagc gttttgtcac cctgcgcgta
     1741 tctgctaaag gtatgcgtgt aatcgataaa aaaggcatcg atacagttct ggctgaactg
     1801 cgtgcccgtg gcgaaaagta ctaagtactt agaggaaata aatcatggct aaaggtattc
     1861 gtgagaaaat caagctggtt tcttctgctg gtactggtca cttctatacc actacgaaga
     1921 acaaacgtac taagccggaa aaactggaac tgaaaaaatt cgatccagtt gttcgtcagc
     1981 acgtgatcta caaagaagcg aaaatcaaat aattctcgct ttgatgtaac gaaaaacctc
     2041 gctccggcgg ggttttttgt tatctgcttg cccccatatt gattgcatct gttcattcct
     2101 ggagatgcta tgcctgaatt acccgaagtt gaaaccagcc gtcgcggcat agaaccgaat
     2161 ctcgttggtg caaccattct tcatgcggtg gtgcgcaacg gacgcttgcg ctggccggtt
     2221 tcagaagaga tctaccgatt aagcgatcaa ccagtgctta gcgtgcagcg gcgggctaaa
     2281 tatctgctgc tggagctgcc tgagggctgg attatcattc atttggggat gtctggcagc
     2341 ctgcgcatcc ttccagaaga acttccccct gaaaagcatg accatgtgga tttggtgatg
     2401 agcaacggta aagtgctgcg ctacaccgat ccgcgccgct ttggtgcctg gctgtggacc
     2461 aaagagctgg aagggcataa tgtgctggcc catcttggac cggagccgct tagcgacgat
     2521 ttcaatggtg agtatctgca tcagaagtgc gcgaagaaaa aaacggcgat taaaccgtgg
     2581 ctgatggata acaagctggt ggtaggggta gggaatatct atgccagcga atcactgttt
     2641 gcggcgggga tccatccgga tcggctggcg tcatcactgt cgctggcaga gtgtgaattg
     2701 ttagctcggg tgattaaagc ggtgttgctg cgttcgattg agcagggtgg tacaacgctg
     2761 aaagattttc tgcaaagtga tggtaaaccg ggctatttcg cgcaggaatt gcaggtttac
     2821 gggcgaaaag gtgagccgtg tcgggtgtgc ggtacgccga ttgtggcgac taaacatgcg
     2881 cagcgggcaa cgttttattg tcggcagtgc cagaagtaat tcatgcgagc cggatggcat
     2941 accatccggc ataaacgcta cgctaatttt gccatcagcg cctgatggac attctccggc
     3001 aggaaatggg tgacatcgcc ctgatggcgc gccacctctt tcaccaacga tgaagagata
     3061 aacgaccact ctttcgacgg catcagaaac acactttcca gttccggcat taagtggcga
     3121 ttcatatgcg ccagctgcat ttcatattca aaatccgcca ccgcacgcag gccacgaatc
     3181 agcaccgtag cgtgttgatt acgggcgaag ttcgccatca aatcactaaa cccgaccact
     3241 tccacgttcc ccagatgcgc ggttgcctgc tgtgccagtg ccacacgctc ttccagggta
     3301 aacatcggtt ttttactggg gctggcggca atcgccagaa taacgtgatc gaacatctgc
     3361 gtggcgcgcg tcacgatatc gatatgacca ttggtaatgg gatcgaaagt acccggataa
     3421 atcgcccgtt tttgcataac aacctcaatg cgttttcggt ggcaggtaag gttccagcag
     3481 ttgaagcaga cgctgtagcg cgccctggtt ttgatacagt acttcaacgg catgacggcc
     3541 atagaaacta cggtaaacgg cgtcggtgag taaagaggaa acctcttttg caagcgtagt
     3601 ggcatcggta acggtaataa gcccgcttgc ctgctccagc cgcgcgcaaa tgtctttaaa
     3661 gttaaaagta tgcggcccca tcaataccgg aatagcgtgt gcggcagctt ccagcggatt
     3721 atgcccacca cgttcaacca gtgaaccgcc aacaaaggcg agatcggcaa tgccgtacag
     3781 taacatcaac tcgcccatcg tatcgccaac cacaacctgc gtgctggtgg aggggacttc
     3841 ccctgaagag cgtgtgatat agcttagtcc agcctggcgg acaaggttaa tcgcatccgg
     3901 gaagcgttct ggatgacggg gtaccaggat gagcaataaa ttcgggaatt gctgtaacaa
     3961 tgcctgatgt gcggcaatga ccacgctctc ttcaccttcg tgagtgctgg tggcaatcca
     4021 taccgggcgg tgtggtgccc attggcggcg tagcgtcacg gctttagcag ccaactgcgg
     4081 agttacagaa atatcgaatt tcaggctacc ggtaacggtt acctggttat tttttgcgcc
     4141 cagcgccaca aaacgtgcac catcttcttc attttgcgca gcaatcagcg taatacgacg
     4201 cagcaagcgg cggacgaatt tacccagttt ggcataacct gcggccgagc gggcagagag
     4261 tcgcgcgtta gcgatcacca gcggaatttt acgtttatgt agcgccgcaa tcaggttagg
     4321 ccatagttcg gtttccataa tcaacaccag tttggggtcg actttattca ggaaacggtt
     4381 gagcgcatcg ggcaggtcat acggcagata aacgtgctga acatccttcc cgaaagccga
     4441 ttgtacgcgc tccgaaccgg ttggcgtcat ggttgtaacg gtaatcggta aatcaggata
     4501 acgatgacgc agcgcgcgca ccaacgggat cgccgccaga gtttcaccga cggagacgga
     4561 gtgcagcata atgccgcctg gttttagcgg atggcggtaa aaaccgtaac gttcacccca
     4621 gcgttttcga taggccggag ccttacgtcc gcgcacccag agccgtatcc agatcagcgg
     4681 ctgaataagg tagagaaggg cggtgtaaag caattcgagc atagtaaata gctgacttat
     4741 ggatgtgctg gggattctat gtatttagct gtggctttac cattactttt c
//
LOCUS       DADLXX010000199         4739 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLXX010000199 DADLXX010000000
VERSION     DADLXX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.203"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..109)
                     /locus_tag="JNX27_003876"
     CDS             complement(<1..109)
                     /locus_tag="JNX27_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAY7884887.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1168)
                     /locus_tag="JNX27_003877"
     CDS             complement(179..1168)
                     /locus_tag="JNX27_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7884888.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAH
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1965..2468
                     /locus_tag="JNX27_003878"
     CDS             1965..2468
                     /locus_tag="JNX27_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7884889.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2548..2799)
                     /locus_tag="JNX27_003879"
     CDS             complement(2548..2799)
                     /locus_tag="JNX27_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7884890.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="JNX27_003880"
     CDS             complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="JNX27_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7884891.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3263..3586
                     /locus_tag="JNX27_003881"
     CDS             3263..3586
                     /locus_tag="JNX27_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884892.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3757..4254
                     /gene="ftnA"
                     /locus_tag="JNX27_003882"
     CDS             3757..4254
                     /gene="ftnA"
                     /locus_tag="JNX27_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7884893.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4291..4530)
                     /locus_tag="JNX27_003883"
     CDS             complement(4291..4530)
                     /locus_tag="JNX27_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7884894.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
BASE COUNT         1293 a         1128 c          992 g         1326 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgtaaa
      301 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
      361 cggttgcctg tgctttagac agttcgctta ctgcatccac accgttaata ccaataccga
      421 tgatatcggc tgctttaaag ccctgacctt cggttgcacg tacgccgcca agtacggtgc
      481 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
      541 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
      601 tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg
      661 tatccagttc gttggcggta atcgccatca ctgcgctttc tttgacatcc cagccacgtt
      721 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc gccgccatca
      781 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccactg
      841 caatgacttt catatcgtag ccatgcgctt tcgcgacgat ggcagagccg agtttcgggt
      901 ccggagtaca aataacgaaa ccttttgcgc cactggctgc caggctgtcg atcgcgttca
      961 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
     1021 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagaa
     1081 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
     1141 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
     1201 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttatcta
     1261 aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca taggagagca
     1321 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
     1381 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
     1441 tggaaatcct tgttgtcttc ggtacccatg cgggatgtct tctttttaac cagtcaatag
     1501 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
     1561 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
     1621 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
     1681 tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa agcactatca ttaaaattca
     1741 ttgcatttac attgatttca ccaatgaaat gtaaaaatat ataaacttga tgatttaagc
     1801 attttcttat aaccgttcag acgttattct tatttcagat catcgtcaga attgactcca
     1861 cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg
     1921 atttatcttg cacctctcca cttctggata taaggatatt aggtatggca accgctggaa
     1981 tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc
     2041 acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc
     2101 aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg
     2161 ctacccccat cgttaaagcc attgatgttc caggtgaaaa actgaactct ctggaagaac
     2221 tgttccaaaa aacgatggaa gaatacgagc aacgttcaag cacgctggcg cagttagccg
     2281 atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag
     2341 aacagcagca tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac
     2401 ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg tcacaccagc
     2461 tgcattgatc atacatcggc gctaatacat tgcgccgatg aagtttttga gaaaccgctg
     2521 cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag
     2581 ttcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg
     2641 atgatgagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
     2701 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag ctaccacatc
     2761 agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt
     2821 tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag
     2881 gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac
     2941 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
     3001 cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa
     3061 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
     3121 aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc
     3181 gcctgccagg catcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt
     3241 ctcttactct tctggctgga ccatgagact tctgattctg actctttcat taataacgct
     3301 ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat
     3361 tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag
     3421 tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcga tcccctttgg
     3481 tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac
     3541 tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc
     3601 gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt
     3661 taataattga agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg
     3721 ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
     3781 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
     3841 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
     3901 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg
     3961 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
     4021 gaaacctata aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca
     4081 atgaccaatc aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat
     4141 gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc
     4201 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
     4261 ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt
     4321 cgaaaaacct tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc
     4381 tcccgctgtc atcccttctg ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc
     4441 tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt
     4501 taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc
     4561 gattaccgct tatgaagact ttgcgccagc gcaggactga atgcttttat tgtacattta
     4621 tatttacaca atatgtaacg tcggtttgac gaagcagccg ttatgcctta acctgcgccg
     4681 cagatatcac tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aaccctggg
//
LOCUS       DADLXX010000200         4735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLXX010000200 DADLXX010000000
VERSION     DADLXX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.204"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..181
                     /locus_tag="JNX27_003884"
     CDS             <1..181
                     /locus_tag="JNX27_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7884895.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            524..1240
                     /locus_tag="JNX27_003885"
     CDS             524..1240
                     /locus_tag="JNX27_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7884896.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1696..1771)
                     /locus_tag="JNX27_003886"
     tRNA            complement(1696..1771)
                     /locus_tag="JNX27_003886"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1736..1738),aa:Asn,seq:gtt)
     gene            complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="JNX27_003887"
                     /pseudo
     CDS             complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="JNX27_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3504..3579
                     /locus_tag="JNX27_003888"
     tRNA            3504..3579
                     /locus_tag="JNX27_003888"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Asn,seq:gtt)
     gene            complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="JNX27_003889"
     CDS             complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="JNX27_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY7884897.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4669..>4735)
                     /locus_tag="JNX27_003890"
     CDS             complement(4669..>4735)
                     /locus_tag="JNX27_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen assimilation regulator"
                     /protein_id="HAY7884898.1"
                     /translation="ELLMSVISSPVMEKRQWQLVS"
BASE COUNT         1438 a         1110 c          970 g         1217 t
ORIGIN      
        1 tgtttcagat aaaccgttac atggtgagat taagcttccc ggccaggcta accgttttta
       61 tgaaggcgct atttccgaac acctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc
      121 agaaggcgac cgactgcatt cacgtaaatt acgaaccttt aatgagccgc cgttccgata
      181 ataagttatg aaccttctta tcagaaatag atgtgaagga ttaagtaaga catctggcaa
      241 atagcctgca ataaacgcgg gctgcttgct tccttgggca gatgcgagtt ttattatcgt
      301 cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta taaaaaatcc
      361 cgccaacaac attagttttt ataaaattaa atataagatt atggctttag aatattttta
      421 tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac acatgttaac
      481 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
      541 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      601 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      661 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      721 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      781 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      841 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      901 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      961 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
     1021 taatattgtt atttatactg aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc
     1081 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
     1141 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
     1201 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatttta aagaaatctg
     1261 tctttatggc agatttcttt aatctcatat aattcttata aaaaatataa tgtttaactc
     1321 gtcatattgc ttataaaaaa tccccgttcc cagagaaata atatttatta aaatggcagg
     1381 tcttcttttt ctgattaccg aagacaaagt ggcaattacg tatagtttcc cgataaagac
     1441 gcgacagcta catcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
     1501 acagaatttt tgaccttgtg aatcgtgatg tctgttgggg aggggcaatt tgctcactga
     1561 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggc cactttttta
     1621 caaagtggcc gggtaaaaat aatttttatt ttttaactgt cctgagtctc atagagatgt
     1681 ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
     1741 tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat
     1801 gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg
     1861 cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg ctcggatcga
     1921 gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca
     1981 gcccagtagg caaacattcc catccagaca ccaaccacac cccagccaag catgattccc
     2041 tgcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac ccacatggcg
     2101 taacgggcat cacgagcacc cttaaatcca gcgggtagca cccatgaggc ggaccaaata
     2161 ggcataaata aagcatttag ctaaatcaga atcacaacga cgtgtttaac ctgtggatcc
     2221 tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt
     2281 aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc
     2341 cctaccccca accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt
     2401 aagttgataa gagccgcaat tgagaacgcg ataaaatttc cggcaataac gctggtcccc
     2461 atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca
     2521 ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa
     2581 tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt
     2641 gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac caggagaaaa
     2701 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     2761 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg
     2821 cagcggctgg ataactgagt acggtcagct ccagataagt caacgccagt gctttaactt
     2881 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaattagcg
     2941 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     3001 ctcgtcgtcg atcccgctta ccgagactaa atgctacaac gacagtagta ccaagatcga
     3061 tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaacccc acgccggcca
     3121 tcgcatcttt tcccagccag ctgaccagaa aagtgctcag gacccccatc aacaggacac
     3181 aggcgttctc catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt
     3241 tgtagctctt gcgtttagag tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     3301 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat gatgagggag
     3361 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
     3421 atagaaacgg caaacagttg ggaaatttaa aaatcgggtt tgacaaaata tttttcgccg
     3481 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     3541 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     3601 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     3661 ggcgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccaaacat
     3721 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     3781 gccgcgtatc caggcggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     3841 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgtgcac
     3901 ttaatacaat atctgccggc aaacctttgc gggtaaatgc gtcatcagtg cgtgagcgcc
     3961 ccgtaattcc ctgtcggtaa gtaattaacg gccacttcgc tattgattcc agcgtcaatg
     4021 gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc caacaaaacc
     4081 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     4141 cttcgccatt ttgcaacaac gtcgcaattt cctgtggcga accctggatt agctcgagcc
     4201 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     4261 gagtatgcgt cgttgcaata gtgagaacac cagacgtatc gttggtaaac aggtctgcaa
     4321 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     4381 ccggttcagt catccccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     4441 catcctccag ttcccgaata tgacggctaa cgcctgactg tgaggtaaaa agcatattcg
     4501 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     4561 aattcacggt aaactccggg cagttcagat atcccgttat tgttaaagtc taatgcccgg
     4621 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     4681 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actct
//
LOCUS       DADLXX010000201         4612 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLXX010000201 DADLXX010000000
VERSION     DADLXX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.205"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..198
                     /locus_tag="JNX27_003891"
     CDS             <1..198
                     /locus_tag="JNX27_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7884899.1"
                     /translation="FLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQLIGIGVLGE
                     YIGRIYLETKSRPRYLIKSRK"
     gene            211..1476
                     /locus_tag="JNX27_003892"
     CDS             211..1476
                     /locus_tag="JNX27_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY7884900.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(1543..2166)
                     /locus_tag="JNX27_003893"
     CDS             complement(1543..2166)
                     /locus_tag="JNX27_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884901.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(2908..3255)
                     /locus_tag="JNX27_003894"
                     /pseudo
     CDS             complement(2908..3255)
                     /locus_tag="JNX27_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            3377..3637
                     /locus_tag="JNX27_003895"
     CDS             3377..3637
                     /locus_tag="JNX27_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY7884902.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(3655..>4612)
                     /locus_tag="JNX27_003896"
     CDS             complement(3655..>4612)
                     /locus_tag="JNX27_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884903.1"
                     /translation="RRLVGDALHKNSGPYKDADRAALSRLYGSLADDQKAALSETNAL
                     RDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQAMSKGDAKGFRELMQNT
                     PSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLANGQMRNLARHLPKETMS
                     GLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFVANHAQRIGTAVGSTVS
                     GPFSAVGAVAGSEIGAKVASKIRAMGGAESIESAEKLISSPEFQKAARLAVKQAPESI
                     VDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK"
BASE COUNT         1298 a         1006 c          884 g         1424 t
ORIGIN      
        1 tttttatatg gtgcatggat gattatagac accattgtct ttggtaaccc agtacgcggg
       61 tatccctcca tgcttgtatc aatacttttc ctgggtggag tgcaactgat cgggattggt
      121 gttctcggtg aatatatagg tagaatttat ttagaaacaa aatcaagacc acggtactta
      181 attaaaagca gaaaataaca ggatgatctt atgaagatat tcataatgcg caattggcac
      241 aagatatcaa tttttatact tgcatttact ttgatttggc ttaggcgcat tgacattttg
      301 acaaatgccc agttttgggc tgaggatgca gttttttggt ataaagatgc atacgaacaa
      361 ggttttttca gttcactcat aaccccaaga aatggttatt ttcaaacagt atcaacactc
      421 attgttggtg cgacgacatt tattaatcca atttacgcac cattattatc aaactttttt
      481 ggaataataa ttagagctat aattatatgg ttcttattta ccgatagatt taaattccta
      541 agcaccacat caaaaatctt catctctgta tatttaattt gcatgcccgg gcttgatgaa
      601 gtccaagcca atataacaaa tgctcactgg tatttatcat tgtatgttgc aatgataata
      661 atatctgaag aatccaagtc aaaattatgg aaagcacatg atttattttt tatagtatta
      721 tctggattga gcggtccatt tattatattc ataattgcat catctgtttt taaatattta
      781 cacttatcaa aaggagtaat aagcataaga ggtttatact tattttatac tcgactgcca
      841 tatttggcaa tgatcttgtg tgggctgata caggcaacat caataattct tacatttaat
      901 ggaacaagat cacatgctcc attaggattc agttttgatg ttatgtcatc tataatatca
      961 tctaatgtat ttcttttttc atttgtccct tggaatatcg ctaatgctgg atgggataat
     1021 caccttttat cgtacacact atcaacttta gtaattacat tatgtgtttt catttatatt
     1081 aaaggaaatt ggcaaatgaa agtgtttgcc acccttccaa tacttattgt cgcattttct
     1141 atggcaaaac ctcaactggc agattctgtt cctcaactgc caacacttgc aaccggaaac
     1201 gggtcaaggt attttgtaaa tattcatatt gcaatttttt cattaatatg cgtttgtttg
     1261 tttcaatgca taaagaataa aaccttaaaa atattcttta aaatctatgt ttcagtcatc
     1321 cttattgcaa tgataaaatt aaactttttt ataacccctc ttcctgacat gaactggtct
     1381 caaggagcag aattaataaa taaagcaaaa catggagaag ccgtctcatt tacagtgctc
     1441 cctccatggt taactcttga tttaataaaa aaataaaact tcacattaat aaataacaat
     1501 gaatccgcct ccctacgtat tcccgtaggg aggctgtcgt gtttatagta gcctggtaat
     1561 gctaaatgca aacgaactgg agattcctgc tccgctaagc gtattgcgca gggtcaccct
     1621 aaatgtatct acaccaatat aagtaacatc acaactaact gaatcaggcg cagatatagg
     1681 tgttatctga accacataac cattagtagc tgctgcgagc ggatgagaaa acgttacatc
     1741 atacacacca gtagcatgcg cgttaacgga tgaaacaccc ggcgcaatgt ctacactacc
     1801 attggcgtta attctccctg agcgcgtaac tgactctgca gaaaccgatg tggccttact
     1861 ggttatttcc ataaaggtgc aaccaatatc gatcaattcg caattgttcc ctgttatcca
     1921 gtatggtcga tctgcacttg gctgataatc ccctgcgctc tggaacgttg tgccattgag
     1981 taagactata acttttcccc cacctgagct tgttacttgt atattcgtgt gcgtgtagcg
     2041 ttcactgctc acgcgatgaa aatttccacc gtttataact accgttacag gtcgcgtccc
     2101 agtattatct atagcaaggt ctgccccccc ggcattagcc tcgaagtatg ggttgtttaa
     2161 caccaaagca cacgaaccgt taaggccatt tatggctaat tgcgcaccac ctgtaccgga
     2221 atccccctga gtaccatttc cttcacaagt caggctgttg atagtagttc ctgcccccca
     2281 tctatcacca agtatacccc atcgtgtatt ctggcttgca gttaatcggt taactgtaat
     2341 ggcattaagc tctgaccctt cactggagtt aagtgcgcgc cagccaatac ggttaccctg
     2401 taagtacaca ttatctaact ctgacgttaa caccgagtct gtacgcaacc cttcgccggt
     2461 agtgtgccca taagacacaa tgttctctaa tttagtgtat gctttattgg ttacgagaag
     2521 accgtaagac gatcccgaac ctttaagtgt aatgtccccc agataatcat aggcgtgtac
     2581 acgttgggtt gccccgactg tatcaccgag catctttaag ttgaaatccg aggcatggca
     2641 gttaaatatc gtttctgcca tattttctcc ggaataatga gcacggactc ctgtcgaacc
     2701 aataaattta gcgtcatagt tgccgtaatc aagaacgagt ggtgacgcga tatcgtacac
     2761 cccttgcttt atttttaatg ttccaccgaa aggccacgat gtaagcgggt cttgatatat
     2821 ttcatttttt attgcaaata gctcctcttg gagatttttg ccgctattag tgccaatgtg
     2881 agcagctcct tcatctgatt gaagtaactg ctttaactga tcaggatcgt acttcagcac
     2941 attctgaaaa tagaactgct gcgaaccata cgcatcatat acagccattg aatggccttg
     3001 ctcagttaca aatttggcaa tctgcccgtt gtacacagga tatccggcag cattgataac
     3061 aattggctga gaaacaggga cgtgagatcc gtcttcattc tctacataaa cctgaatctg
     3121 gttttcagga tttaccgggt cagtgtcaat ttttccgata taaattttgc cattggatac
     3181 ggctttaaaa gaacgcgcca tagtgaagag ttgcgaaggc atgctgacca caacatttgc
     3241 ggcgatatct gacatttcat tgctccagac gaatgatatg atgcaatcat gatgtgattg
     3301 cataccgaaa tggcactatt gaatatttat ccagtaggtt acgatgccat tccacccaac
     3361 tggtgaggca tcaaggatgt acagcaaata cgacgaggca cagtttcact tgagacttcc
     3421 gcatgaactc cacgcgaaaa ttaaacagtg tgcgaagatg aataacagtt cgctgaactc
     3481 agagataatt gcagcgattg aagaatcatt ggctaaacaa agctctgcat cagtttacat
     3541 tgacgatgca gagcgtatgg cagaacaaca atctgatatg gttaagaaaa ttgtctttga
     3601 tacgctcaag gagctatata aaaaagacag caactaacca tccgtggctt tccgttactt
     3661 ctggttatca tcactgttca tccagtacat gatgcctagc cttgatatgg ttttcttatc
     3721 tgattctgga agtgagttgt aaaacgagcg ccaagcagaa gagcgtctta cagttgtatc
     3781 aacgatgctt tctggtgctt gttttactgc cagccttgct gctttctgga attctggtga
     3841 gctaattagc ttttctgcag attcaattga ttcagcgccg cccatcgccc tgattttgct
     3901 ggcgactttt gccccaattt ctgacccagc aacagcacct actgcactga acggtcctga
     3961 cacagttgaa ccaaccgcag tgccaatgcg ttgggcatgg ttagcaacaa attcatttgc
     4021 cgcagtgacg cgattaaacc gtttgacgaa ctcgtttagt cttcctgtag ttatctcgta
     4081 agattttgca tctttgatag cctttgcgac cttatataca tcgttcagac ctgacatagt
     4141 ctcttttggt aaatgtctgg caagattgcg catctgcccg tttgctaaca tgttttgata
     4201 ccagtcagca aaccctgcag gattaaaatc agcgccacgt tttccgttcg aaagcatatc
     4261 ccgaagacct gttcccagta gctcggttct tagcttcctg gacggcgtgt tttgcatcaa
     4321 ttcacgaaat cctttggcat cgcctttcga cattgcctgt agtgctgtag ttgctttgcg
     4381 agaaacatca ccgttcagcg ttctgccagt tagattaatc atttgctctt ccatgctttt
     4441 tcgcatctga acaagcctct gagcaacttc aaaatcacgt aatgcatttg tctctgacag
     4501 cgccgccttt tgatcatcgg cgagcgaacc gtaaagcctc gataaagcag cgcgatcagc
     4561 atctttatat ggtccagaat tcttatgaag tgcatcacca acaagtcgtc gt
//
LOCUS       DADLXX010000202         4608 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLXX010000202 DADLXX010000000
VERSION     DADLXX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.206"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..81
                     /locus_tag="JNX27_003897"
                     /pseudo
     CDS             1..81
                     /locus_tag="JNX27_003897"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            316..1224
                     /locus_tag="JNX27_003898"
                     /pseudo
     CDS             316..1224
                     /locus_tag="JNX27_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
     gene            1224..1958
                     /locus_tag="JNX27_003899"
     CDS             1224..1958
                     /locus_tag="JNX27_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAY7884904.1"
                     /translation="MVNDLTYKKLQSTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRNERCNNMRTKQKV"
     gene            2426..3187
                     /locus_tag="JNX27_003900"
                     /pseudo
     CDS             2426..3187
                     /locus_tag="JNX27_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            3231..4310
                     /locus_tag="JNX27_003901"
     CDS             3231..4310
                     /locus_tag="JNX27_003901"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7884905.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMGYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQHFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..4594
                     /locus_tag="JNX27_003902"
     CDS             4307..4594
                     /locus_tag="JNX27_003902"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7884906.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQLLDLPLYFQTSVIT"
BASE COUNT         1262 a          884 c         1126 g         1336 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtgaccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagtgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttacaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggaatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tctgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgtagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt ccgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttacat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggggt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagcatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa agcgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat tgttggattt
     4561 gcccctatat ttccagacat ctgttatcac ttaacccatt acaagccc
//
LOCUS       DADLXX010000203         4579 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLXX010000203 DADLXX010000000
VERSION     DADLXX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.207"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..213
                     /locus_tag="JNX27_003903"
     CDS             <1..213
                     /locus_tag="JNX27_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7884907.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JNX27_003904"
                     /pseudo
     CDS             356..577
                     /locus_tag="JNX27_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(598..660)
                     /locus_tag="JNX27_003905"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JNX27_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="JNX27_003906"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="JNX27_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7884908.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="JNX27_003907"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="JNX27_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="JNX27_003908"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="JNX27_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7884909.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLNPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1604 a          815 c          851 g         1309 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg cgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatggctct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ctttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttaacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttga
//
LOCUS       DADLXX010000204         4549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLXX010000204 DADLXX010000000
VERSION     DADLXX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.208"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            132..575
                     /locus_tag="JNX27_003909"
     CDS             132..575
                     /locus_tag="JNX27_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7884910.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /locus_tag="JNX27_003910"
                     /pseudo
     CDS             811..1630
                     /locus_tag="JNX27_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNX27_003911"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNX27_003911"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7884911.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNX27_003912"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNX27_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7884912.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNX27_003913"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNX27_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7884913.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKANEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /locus_tag="JNX27_003914"
     CDS             complement(4321..4470)
                     /locus_tag="JNX27_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7884914.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1233 a         1188 c         1084 g         1044 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 actacttgta atcaattgat tataaaggat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgttggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagggtaatg ctgccaactt actgattta
//
LOCUS       DADLXX010000205         4508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLXX010000205 DADLXX010000000
VERSION     DADLXX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.209"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            368..2020
                     /locus_tag="JNX27_003915"
     CDS             368..2020
                     /locus_tag="JNX27_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7884915.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JNX27_003916"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JNX27_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JNX27_003917"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JNX27_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2853)
                     /locus_tag="JNX27_003918"
     CDS             complement(2527..2853)
                     /locus_tag="JNX27_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7884916.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
     gene            complement(3138..3790)
                     /locus_tag="JNX27_003919"
                     /pseudo
     CDS             complement(3138..3790)
                     /locus_tag="JNX27_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
BASE COUNT         1198 a         1089 c         1038 g         1183 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctttcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc tcctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctctgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgaggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgcttcg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgta
//
LOCUS       DADLXX010000206         4438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLXX010000206 DADLXX010000000
VERSION     DADLXX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.210"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..94
                     /locus_tag="JNX27_003920"
     CDS             <1..94
                     /locus_tag="JNX27_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAY7884917.1"
                     /translation="MRALRKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            242..1006
                     /locus_tag="JNX27_003921"
     CDS             242..1006
                     /locus_tag="JNX27_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAY7884918.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(1499..2083)
                     /locus_tag="JNX27_003922"
     CDS             complement(1499..2083)
                     /locus_tag="JNX27_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7884919.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(2083..3321)
                     /locus_tag="JNX27_003923"
     CDS             complement(2083..3321)
                     /locus_tag="JNX27_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7884920.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(3318..4223)
                     /gene="mph(A)"
                     /locus_tag="JNX27_003924"
     CDS             complement(3318..4223)
                     /gene="mph(A)"
                     /locus_tag="JNX27_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAY7884921.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(4345..>4438)
                     /locus_tag="JNX27_003925"
     CDS             complement(4345..>4438)
                     /locus_tag="JNX27_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAY7884922.1"
                     /translation="MRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT          933 a         1368 c         1363 g          774 t
ORIGIN      
        1 gatgcgtgca ctacgcaaag gccaggcctc agcattttat tatggtgatc ccctgggcga
       61 aatgcgcctg gtaagcagag tttttgaaat gtaaggcctt tgaataagac aaaaggctgc
      121 ctcatcgcta actttgcaac agtgcctgca ccaatctcga ctatgctcaa tactcgtgtg
      181 ggctctgttg caaaaatcgt gaagcttgag catgcttggc ggagattgga cggacggaac
      241 gatgacggat ttcaagtggc gccatttcca gggtgatgtg atcctgtggg cggtgcgctg
      301 gtattgtcgc tatccgatca gctatcgcga ccttgaggaa atgctggcgg aacgcggcat
      361 ttcggtcgac catacgacga tctatcgctg ggtccagtgc tacgccccgg agatggagaa
      421 gcggctgcgc tggttctggc ggcgtggctt tgatccgagc tggcgcctgg atgaaaccta
      481 cgtcaaggtg cggggcaagt ggacctacct gtaccgggca gtcgacaagc ggggcgacac
      541 gatcgatttc tacctgtcgc cgacccgcag cgccaaggca gcgaagcggt tcctgggcaa
      601 ggccctgcga ggcctgaagc actgggaaaa gcctgccacg ctcaataccg acaaagcgcc
      661 gagctatggt gcagcgatca ccgaattgaa gcgcgaagga aagctggacc gggagacggc
      721 ccaccggcag gtgaagtatc tcaataacgt gatcgaggcc gatcacggaa agctcaagat
      781 actgatcaag ccggtgcgcg gtttcaaatc gatccccacg gcctatgcca cgatcaaggg
      841 attcgaagtc atgcgagccc tgcgcaaagg acaggctcgc ccctggtgcc tgcagcccgg
      901 catcaggggc gaggtgcgcc ttgtggagag agcttttggc attgggccct cggcgctgac
      961 ggaggccatg ggcatgctca accaccattt cgcagcagcc gcctgatcgg cgcagagcga
     1021 cagcctacct ctgactgccg ccaatctttg caacagagcc cgccgtgcta gtctgctcgg
     1081 tgatggtgga gtgaagccaa cccgcaatcg ggttatgaat ctgcatcgcg attcgcaatc
     1141 agctgtctct tgagcatgtc gaactcctgc gatgttatct cgccgtgctc gtgctgctac
     1201 accaacttgt ccaactcatc cgccacaccg ctccccacca cctgcgatgg ccaggttgtt
     1261 tgctgggcgc attggcggag tatcgtctcg acccgggaca cccacaccgg cacaacctta
     1321 cgggagtgat tcactgtcaa agaatcggcc cggtgctctg acgcaagtat cgggatggtc
     1381 accatttgta agccgtagac ctgagtggtg atcaagactt cgataccacc gaccgtaccg
     1441 gtactaatcg acgacggtcg tgttcgtcgc ctgccgcagg gactctgcac acctccgttt
     1501 acgcatgtgc ctggaggagt tggaaatcgt cgtgttcggg aaacattaaa cacaggatgg
     1561 cagcgatctg agccagcaca tgatcagcta gctcaccatc cggatcgacg gcccactgca
     1621 tcgtcgcgcc agcgatgacc gagtgcagga gcaactcagc tgccgcagga gcacctgggg
     1681 gcagtcgctt gcggatcccc tccaccaccg cgcggttccg ctggatcgca agcgtgcgta
     1741 gctccggcac ctggagctcg taccaggaga tgagatagtt caccgagaag tcgttgcgag
     1801 tgttcatgct ccgaacgagc acctgcaaaa attcccagag cccttgcggc cctgcgccta
     1861 tcggtatcgc attcaggtaa tgccgcacct gctcgacgcc gcgctccatc atcctcacca
     1921 gcagcgtatc gcggttggtg aagcgctgga ttaacgctgc gcgggagagc cccacctcct
     1981 ttgctactcc gctgagcgtg aactctatgg gaccgcaacg cttcagcact acggtggcgg
     2041 cctcgagtac ctcgtcatcg gacttgagct tggggcgggg catcagtgtt caccttctgt
     2101 atgggttggg gcggaggctg tggctgccgc cgccattgta gcaaattgaa gacggagcga
     2161 gagtagagcc acgagccccg caaacacggc cgatacaacg aggccaggga gcggaccagc
     2221 aaggtcgaca aacgcgccgg ccgcaagcat aaccatgggc gaggctgaca gcatcaccgc
     2281 cgagaccgtg ccgagtaccc ggccgagaag ttctggcggc gtgcggttgt agatggcagc
     2341 gttgagaatg ggagagactg agccggtcag cagtcccacg agcgcgccca acaacatcag
     2401 caccggcacg cctggcaact gtgaaagcag aagcgagccc accgcagagc cacaaaatgc
     2461 caccgccagc cagttctgcg ctgatatccg ggcgccgacc gacgcatgaa tggcaatgcc
     2521 aaggagacca ccagccccca tcattgagga gaacagcccg agctctgcta cttggcgtcc
     2581 tgcatctaca aacagcgcag gcatgatgac gctgccgttg gcgccaacga tgcccacgaa
     2641 gatcatcact ataccaaaga gagggcgcag caggggttcg ctccagagaa aagcgacgcc
     2701 ggcgcgcatg gagagagtcg ccgtcgtggt catcgtccga gcggcacgcg cgggaagcac
     2761 ccacgcgccg agcagacctg caaggacgga gcagaacgcc gtcagcccga gcgttggcgc
     2821 agcgccaagc aggccgattg cggccccccc aagggccggg ccacctagaa tcgcgacgtt
     2881 cccgatcacc gctttcagtg acgagacgcg ctcaacggag agcccggcga cgtggccgag
     2941 tttgggcagc tcactgtcct gcgcggccat accgggtgcg tcgaacgcgg caccgagcac
     3001 cacgcaagcg atcagcccag tgttcgagag ggcgccaacg gcatcgagca gtgggatgct
     3061 cgccatggcc acgccgccca ccacacccga gatcaatgcg acgggcgcgc gcccgaaccg
     3121 atcgacgagg ccaccaccaa cccacgcgcc gatgatggtc gcgatgacgc tgctagcggc
     3181 cgtggcgccc gcccaggccg cgctctttgt atgagacagg acgaaccatg gaagcgcgag
     3241 ggccgccacc gcgttgccga tccggaagag aaaggtcgcc gcgaacagcg tcgcgagcgg
     3301 gctatatcga cgttcgctca ttccgctgcg gcgagctgcg ccttcgccgc agcgaggtac
     3361 tcttcgttac ccgagtcgag ggcgaagagt gcgtaggtga ccgccccgaa cgcaaggcgc
     3421 tccgcgatgt ggtgggcgag ccgcggccac acccggccac cggccgcttc atacgtgagg
     3481 aggagcttcg cgagcccctc ttcaccaaag accataaggt gcgcggccat gtcgatggca
     3541 gggtcatcaa cgcgggcctc gctccagtcg atcatcccgc tgacgcgctc cgtgttgtcg
     3601 atgagcacat ggcccacgta gagatcgcca tgcaccacca cggagaaatc tggccacgac
     3661 gaatcgtcgt cgagccagcg ctgccaccgg tggaggcgct tgtcgttcac cacgaactcg
     3721 cgtcggacgc ggtcaacgtc gtcggccacc ttctgacggg cctgcgtcgg tgtacggatg
     3781 agcatccccg catccacggc ggcggaaatg gggacggcat gcagggcggc gagcgcggtc
     3841 gcgaagctct ccgcgaagac ctccgagtcc tgcggcacga cccagtcggg cgtggacgaa
     3901 ccaggctgga tgaccatcgc agtcgagtct tcgagcatgg gataggcaac gagctcggcg
     3961 ttggccacgc gccagtccgg caccgcgaac ggcaggcgat tcttgagcat tgccagcacc
     4021 cgcgcctctg gttcgacctt cgcgcttacc tcggctcggc gcgggatgcg cagcacccac
     4081 cgacgtccat cgtcgacggt ggcgatcacg atcctatagt cgagcccaag ctcattgaca
     4141 gtcagcgggc catggagctt gagcccatgt cgggctgcaa gtgcgtacag ttgggaggta
     4201 tcggcggtcg tgactacggt catgattcac tcctgagggc ttgacgggtt tagccaccta
     4261 aatgtaacag tcacgtcggt tatattcaat ccggcactgt tgcaaagtta gcgatgaggc
     4321 agccttttgt cttattcaaa ggccttacat ttcaaaaact ctgcttacca ggcgcatttc
     4381 gcccagggga tcaccataat aaaatgctga ggcctggcct ttgcgtagtg cacgcatc
//
LOCUS       DADLXX010000207         4438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLXX010000207 DADLXX010000000
VERSION     DADLXX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.211"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..212)
                     /locus_tag="JNX27_003926"
     CDS             complement(<1..212)
                     /locus_tag="JNX27_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7884923.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPV"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="JNX27_003927"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="JNX27_003927"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7884924.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="JNX27_003928"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="JNX27_003928"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7884925.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="JNX27_003929"
     CDS             2893..3633
                     /locus_tag="JNX27_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7884926.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3668..4189
                     /gene="ruvC"
                     /locus_tag="JNX27_003930"
     CDS             3668..4189
                     /gene="ruvC"
                     /locus_tag="JNX27_003930"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7884927.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4191..>4438)
                     /locus_tag="JNX27_003931"
     CDS             complement(4191..>4438)
                     /locus_tag="JNX27_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884928.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1098 a         1105 c         1233 g         1002 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggccaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcagggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggttacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaattgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgcccggaa atggcgcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgaatttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctgggaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgggatgg
     1741 gcgcactgct cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 ctgagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ctgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gactgtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcacgca
     3241 ttcagcaaat gtggcggtaa cctcggcact gacggttccg tagcttatct gttcagcaaa
     3301 aaaggcgtga tctccttcga gaaaggcgac gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacatatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc gccgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gatgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgaggttgc agcgactctc tgatgatgcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctacggcgt
     3721 catccgccag gtaggtaggc aactgtctta cctgggtagc ggatgcatac gcaccaaagt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcct gattatttcg ccattgaaca agtctttatg gcgaagaacg ctgactcagc
     3901 cctgaaactg ggccaggcgc gcggcgtggc gattgttgcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tacgcggcac gtcaggtaaa gcaaacggtg gtaggtatgg gtagtgccga
     4021 aaaaagccag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgctgg cgattgccat cacccactgc cacgttagtc agaatgcgat
     4141 gcagatgagc gaatcgcggc tgaacctggc gagagggcga ctgcgttaag ttataccgcc
     4201 tcggtcagtt ctggctgagg cgtttcaact ccctccgcgt gaatcaacac caaacccgga
     4261 tgatgccaca aactggctgg cgtgacggtt ttacgctccc acggaataga acctaagaac
     4321 cagaatttcc agaaagtgag ttttgcattc ggattgctat ttaacagttg tccaaacgtc
     4381 tctatttcac ccaccggaat acatagcgcc tctttataaa tatcaaagac aaattttg
//
LOCUS       DADLXX010000208         4378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLXX010000208 DADLXX010000000
VERSION     DADLXX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.212"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(64..822)
                     /locus_tag="JNX27_003932"
     CDS             complement(64..822)
                     /locus_tag="JNX27_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7884929.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JNX27_003933"
     CDS             complement(830..1933)
                     /locus_tag="JNX27_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7884930.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JNX27_003934"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JNX27_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JNX27_003935"
     CDS             complement(3181..4206)
                     /locus_tag="JNX27_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7884931.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1215 a         1146 c         1022 g          995 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatactg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DADLXX010000209         4351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLXX010000209 DADLXX010000000
VERSION     DADLXX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.213"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            333..1391
                     /gene="rfaQ"
                     /locus_tag="JNX27_003936"
     CDS             333..1391
                     /gene="rfaQ"
                     /locus_tag="JNX27_003936"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7884932.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1388..2512
                     /locus_tag="JNX27_003937"
     CDS             1388..2512
                     /locus_tag="JNX27_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7884933.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2505..3302
                     /gene="rfaP"
                     /locus_tag="JNX27_003938"
     CDS             2505..3302
                     /gene="rfaP"
                     /locus_tag="JNX27_003938"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7884934.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3345..>4351
                     /gene="waaO"
                     /locus_tag="JNX27_003939"
                     /gene_synonym="rfaI"
     CDS             3345..>4351
                     /gene="waaO"
                     /locus_tag="JNX27_003939"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7884935.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
BASE COUNT         1233 a          876 c          989 g         1253 t
ORIGIN      
        1 ttaaatagca atttacccgc caattcttgc tgaacatcta tacttttttt gtatttttat
       61 gcgattcatt gatttctgct gtacgtgcag gaaggaatcg cagagcaaca gtgaaagaat
      121 atggttgcta atgattttat ttgattgatt tctttattga aaatcaacaa tgtgcttaaa
      181 aatcattcgg gtcggattgc cgcgaaagaa gtgatacact agcgcgtcaa tatcagtgcg
      241 gtatcagtat tcaggtagct gttgagcctg gggcggtagc gtgctttttc tgcttcacct
      301 taatcggata atctcaacaa aaagagttac ttgtggataa gccatttcga agaattttgc
      361 tcattaagat gcgttttcat ggggatatgt tattaactac tcccgtcatt agttctctga
      421 aaaaaaatta ccctgacgca aaaatcgatg tgctgcttta tcaggacacc atcccgatcc
      481 tgtctgaaaa tccagagatt aacgcgctct acggcataaa aaataaaaaa gcaaaagcct
      541 cagaaaaaat tgccaacttt tttcatctca tcaaggtatt acgtgccaat aagtatgacc
      601 ttatcgtcaa tcttaccgat caatggatgg ttgctatact ggttcgctta ttaaatgctc
      661 gtgtgaaaat ttcccaggat tatcatcatc ggcagtctgc tttttggcgt aaaagtttca
      721 cccatttggt gccgttgcag ggtggaaatg tggtggaaag taacttatcc gtgctgacac
      781 cattgggact tgattcgttg gtgaagcaga caaccatgag ttacccgcct gcaagctgga
      841 aacgtatgcg tcgcgaactt gatcacgctg gtgttggaca aaattatgtg gttatccaac
      901 ctacggcgcg gcaaatcttc aaatgctggg acaacgccaa gttttccgct gtgattgatg
      961 ccttacatgc tcgtggttat gaagtcgttc tgacgtccgg cccagataaa gacgatctgg
     1021 cctgcgtcaa tgaaattgcg cagggatgcc agacgccacc agtaacggcg ctggctggaa
     1081 aggtgacctt cccggaactt ggtgcgttaa tcgatcatgc gcagctgttt attggcgttg
     1141 attccgcacc ggcgcatatt gccgctgcag ttaatacgcc gctgatatcg ctgtttggcg
     1201 caacagatca tattttctgg cgtccctggt caaataacat gattcaattc tgggcgggag
     1261 attaccggga aatgccaacg cgcgatcagc gtgaccgaaa tgagatgtat ctttcggtta
     1321 ttccggcggc agatgtcatt gctgctgtcg ataaattact gccctcctcc acgacaggta
     1381 cgtcgttatg atcgttgctt tttgtttata taaatatttt ccctttggcg gtttgcagcg
     1441 cgattttatg cgtattgctc agacagtcgc cgcccgaggt catcatgttc gggtttatac
     1501 ccagtcgtgg gaaggcgaat gccctgatgt atttgaactg atcaaagtgc cggttaaatc
     1561 gcataccaat cacgggcgca atgcggagta ttttgcctgg gtgcaaaaac atttacgcga
     1621 acatcccgtc gataaagtcg ttggattcaa caaaatgccg gggctggacg tttattatgc
     1681 cgctgatgtt tgttatgccg agaaagtagc gcaggaaaaa ggctttttct atcgcctgac
     1741 gtcacgttat cgccattatg ccgcctttga gcgggcaacc ttcgaacagg gcaagccgac
     1801 acagctgctg atgctgacag ataagcaaat cgccgatttc cagaaacatt atcagactga
     1861 agcggagcgt tttcatattc tgccaccggg gatttatcct gatcgtaaat atagccagca
     1921 gccagccaat agccgtgaaa tcttccgtaa gaagaatgga ataaccgaac aacaatattt
     1981 attgttgcag gtcggttcag acttcacgcg taaaggtgtc gatcgttcca ttgaagcact
     2041 tgcttcgtta ccggattcgc tgcgccacaa cacattgcta tatgttgttg ggcaggataa
     2101 accgcgaaaa tttgaggcac tggcagaaaa acgcggcgtg cgcagtaatg ttcacttctt
     2161 ctcggggcgc aacgatgtct cggaattaat ggcggcggcg gatttattac tgcatcctgc
     2221 ctaccaggaa gcggcgggaa ttgtgctgct ggaagcgatt actgcaggat taccggtact
     2281 aacaactgcc gtttgtggct atgcgcatta tattgtcgac gctaattgcg gcgaggctat
     2341 tgctgagcca ttccgccagg aaacattgaa tgagatttta cgcaaagcgt taacgcaatc
     2401 ttcattgcgc caggcttggg cggaaaatgc gcgacattat gctgatacac aagatttata
     2461 cagtctgcca gagaaagcgg cggatatcat aacgggtggt ctggatggtt gaacttaaag
     2521 agccgtttgc cacgttatgg cgcggcaaag atccttttga ggaagttaaa accttgcagg
     2581 gtgaggtatt tcgtgaactg gaaactcgcc gtactctgcg ctttgaaatg gcgggaaaaa
     2641 gctattttct caaatggcat cgcggcacga ccctgaaaga gataatcaaa aatttactct
     2701 cattgcggat gccagtatta ggcgcagacc gcgaatggaa tgcgattcat cgactgcggg
     2761 atgtcggcgt tgatactatg tatggggtgg cattcggcga aaaaggcatg aatccgctga
     2821 ccagaacctc gtttattatt accgaagatc tgacaccaac catcagtctg gaagattact
     2881 gtgctgactg ggcgaccaac ccaccagatg ttcgcgtaaa gcgtatgctt attaagcgtg
     2941 tcgcgacgat ggtgcgcgat atgcatgctg cgggcattaa ccatcgtgac tgttatatct
     3001 gtcatttcct gctgcacttg cctttttccg gtaaggaaga ggagttaaaa atttcggtaa
     3061 ttgacctaca ccgggcgcag cttcgcacgc gcgttccagg tcgttggcgc gataaagatc
     3121 ttatagggct ttatttttct tcaatgaata ttggcctgac tcagcgggat atctggcgct
     3181 ttatgaaggt gtattttgcc gccccgctta aagacattct caagcaggaa caaggactgc
     3241 tgtcgcaagc agaagcaaaa gccacaaaaa tcagggaaag aacgattcga aaatcgttgt
     3301 aacccgtaac ttatttttgc caaaattttg gatacagaat aaatatgtct caactcaatg
     3361 atagtgacat catccttttt gagtataatt ttcattatca aaatataaga tctaaaaata
     3421 ctcttgatat agcatttggt attgacagaa attttctttt tggatgtggt gtagccatcg
     3481 catctattct attaaacaat agagaaatct cttgtgaatt tcatgttttc acagattata
     3541 tcagtgataa agacaaatta tatttttctg atttagcaaa acaatataat tcaagaatta
     3601 atatttatgt tatcaattgt gataagctga agtcattacc aagcacgaaa aactggactt
     3661 acgcaacata ttttcgattt ataattgcag attatttcta tcataaacat gaaaaaatac
     3721 tatatcttga tgcagatatt gcttgcaagg gtagtattaa agaactctta gattatcaat
     3781 tttctactaa tgaaattgcc gcagttgtag ctgaaagaga tattgaatgg tggcaaaatc
     3841 gagcctcggt attaactaca ccacagttag cttctggata tttcaatgct ggttttttac
     3901 tgataaatat tgatgagtgg aatctaaata acatttcgtc aaaagctatt gaaatgttgc
     3961 gtgacccaga ttgggtaagt aaaatcaccc accttgatca agatgtactg aatgtattat
     4021 tgaatggtaa agtgaagttt atttcggaga aatataatac ccgatatagt attaactatg
     4081 aattaaaaga caaagttgat aatccagtca atgatgacac cgtgtttata cactatgtgg
     4141 gacctacaaa accctggcat gagtgggctg attatccggt ttcacgtagt tttttgatcg
     4201 ccaaagcagc ttctccgtgg agtaaagaag atttacttaa acctgtaaat agcaatcagt
     4261 atcggtattg tgcaaaacat aaatttaaac aaaagcatta tatggcaggc atttttaatt
     4321 atttaaagta ttataaagaa aaatgcttct a
//
LOCUS       DADLXX010000210         4336 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLXX010000210 DADLXX010000000
VERSION     DADLXX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.214"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..171)
                     /locus_tag="JNX27_003940"
     CDS             complement(<1..171)
                     /locus_tag="JNX27_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7884936.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTEVY"
     gene            complement(321..482)
                     /locus_tag="JNX27_003941"
     CDS             complement(321..482)
                     /locus_tag="JNX27_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884937.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(479..682)
                     /locus_tag="JNX27_003942"
     CDS             complement(479..682)
                     /locus_tag="JNX27_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7884938.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            927..1262
                     /gene="iraM"
                     /locus_tag="JNX27_003943"
     CDS             927..1262
                     /gene="iraM"
                     /locus_tag="JNX27_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7884939.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1543..1674)
                     /locus_tag="JNX27_003944"
     CDS             complement(1543..1674)
                     /locus_tag="JNX27_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY7884940.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2174..2250)
                     /locus_tag="JNX27_003945"
     tRNA            complement(2174..2250)
                     /locus_tag="JNX27_003945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Arg,seq:tct)
     gene            complement(2352..2427)
                     /locus_tag="JNX27_003946"
     tRNA            complement(2352..2427)
                     /locus_tag="JNX27_003946"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            complement(2571..3392)
                     /locus_tag="JNX27_003947"
     CDS             complement(2571..3392)
                     /locus_tag="JNX27_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7884941.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3407..3763)
                     /locus_tag="JNX27_003948"
     CDS             complement(3407..3763)
                     /locus_tag="JNX27_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7884942.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3776..>4336)
                     /locus_tag="JNX27_003949"
     CDS             complement(3776..>4336)
                     /locus_tag="JNX27_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7884943.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1188 a         1181 c          858 g         1109 t
ORIGIN      
        1 gtacacctca gtcagatatg tcagcagccc aaacagcaga ctccccagca cgcctattgc
       61 cgcccactga gacggggaaa acctgtccag caactgcagg aaccagtagc ccgttcccac
      121 cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca taccccgtct
      181 cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg atggctctga
      241 taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta acgatacctt
      301 ccatttgact ggctcacttt ttatgatgat gccggtgcat ttatctccag caccagactt
      361 tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag tgcatttagt
      421 cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc
      481 actccggctc agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca
      541 gggtataagg ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat
      601 gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa
      661 atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga catgtaatac
      721 agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga
      781 taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga
      841 aaaataattc tgctctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac
      901 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      961 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
     1021 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
     1081 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
     1141 ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
     1201 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
     1261 aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga
     1321 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
     1381 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
     1441 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
     1501 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt
     1561 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg
     1621 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1681 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1741 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1801 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1861 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1921 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1981 atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga
     2041 ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca aatatataat
     2101 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     2161 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     2221 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     2281 cccgcccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg
     2341 gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     2401 tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg taattaattt
     2461 cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa
     2521 cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt
     2581 acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca
     2641 tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg
     2701 catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc
     2761 tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca
     2821 ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga
     2881 caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca
     2941 cctttcgtgc actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta
     3001 aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct
     3061 gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt
     3121 atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct
     3181 gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac
     3241 gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     3301 atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg
     3361 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg
     3421 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgaacg atattgattt
     3481 catcaaactg ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt
     3541 tcaggatatt gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg
     3601 ccagccttcc ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc
     3661 ggcgataacg ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag
     3721 tgtttaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc
     3781 caagcacgcc agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac
     3841 catgcttttc ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca
     3901 gcggtagcgt gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca
     3961 ggtgatgggg atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc
     4021 gtttgtattt ctcgttaacc cagctgcgac gtttaggtcg tttcatgaaa gattccggag
     4081 actcaggatc aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt
     4141 gggcgtgagg cagcggcgca agattttttg tgcgctgttt cagtatgctg gtggcggtct
     4201 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     4261 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     4321 accaggataa ttcagc
//
LOCUS       DADLXX010000211         4326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLXX010000211 DADLXX010000000
VERSION     DADLXX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.215"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..324
                     /locus_tag="JNX27_003950"
     CDS             <1..324
                     /locus_tag="JNX27_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HAY7884944.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="JNX27_003951"
     CDS             383..586
                     /locus_tag="JNX27_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY7884945.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRELFTCNKTLPPHEVEPNFGAI"
     gene            586..1623
                     /locus_tag="JNX27_003952"
     CDS             586..1623
                     /locus_tag="JNX27_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7884946.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPSNSKCDFCH"
     gene            complement(1651..1740)
                     /locus_tag="JNX27_003953"
     tRNA            complement(1651..1740)
                     /locus_tag="JNX27_003953"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1704..1706),aa:Ser,seq:cga)
     gene            1834..2631
                     /gene="mtfA"
                     /locus_tag="JNX27_003954"
     CDS             1834..2631
                     /gene="mtfA"
                     /locus_tag="JNX27_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7884947.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2732..2807
                     /locus_tag="JNX27_003955"
     tRNA            2732..2807
                     /locus_tag="JNX27_003955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2765..2767,aa:Asn,seq:gtt)
     gene            2969..4231
                     /locus_tag="JNX27_003956"
     CDS             2969..4231
                     /locus_tag="JNX27_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7884948.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1206 a          996 c         1036 g         1088 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gagctattta cctgcaataa
      541 aactttacca ccacacgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagtaatt ccaagtgtga tttttgtcac
     1621 tgacttaatg atgtataagt gattgaattt tggcggagag agggggattt gaacccccgg
     1681 tggaggtgcc cccactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc
     1741 gctcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa
     1801 ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa
     1861 gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg
     1921 tgtctgacag aacaggaaca aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa
     1981 aaacggcttg ttcctttaca gggctttgag ctgaattcat taagaagctg ccggatagca
     2041 cttctatttt gcctgcccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa
     2101 gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg
     2161 gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg
     2221 aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa
     2281 gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg
     2341 ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa
     2401 gaaatcgaat tagttggtga gaatgcggcg agcattgatg cttatgctgc cagtgatcct
     2461 gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct
     2521 cgtttccctt cattgtggca acgtttctgt caattttatc aacaagatcc tttgcagaga
     2581 ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta attaacaact
     2641 ttgcagatta attaaccaat tgaaatggct tatgaaattt agtgttgaca gacaaggtac
     2701 cgctaagtaa tatgcgcccc gttctcacga ttcctctgta gttcagtcgg tagaacggcg
     2761 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaatt ttctgttttc
     2821 atgcatcctt gcgaatcttt atctgatgtt gattcaacag gttagtgtga aaactcttct
     2881 cggtgcattt tgattttacc ctacgcatcg ggaaaaattg gtggtcaaat ctgggatcag
     2941 gttagttcga taatggagtg acccccatat atcccttacc gacgcaaaaa tccgcaccct
     3001 caagccttct gataaaccct ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa
     3061 gccgggtggc tcccgccact ggtatctcaa ataccgtatt agcggtaaag aatcccgcat
     3121 tgcgctgggt gcctatccag ccatctccct gtctgatgcg cgacagcaac gtgaaggtat
     3181 ccgtaaaatg ctggcgctga atatcaaccc ggtacagcag cgggctgctg aacgtggctc
     3241 acgaacaccg gagaaagttt ttaaaaacgt ggcgctggcg tggcataaaa gtaacaggaa
     3301 atggtcgcag aacaccgccg accgtctgct tgccagcctg aacaatcaca tctttccggt
     3361 catcgggaac ctacctgtat cagaacttaa accccgtcat ttcattgacc tgctgaaagg
     3421 gatcgaggaa aaaggtctgc tggaggttgc gtcccgcaca cggcagcacc tgagtaacat
     3481 aatgcgccat gcggtccatc tggagttaat cgatacgaac cctgcagcaa accttggcgg
     3541 cgtgaccaca cctcctgtca gacggcacta tcctgccctg ccgctggagc ggctgcctga
     3601 actgcttgaa cgtattgggg catatcatca gggcagtgaa ctgacccggc atgccgttct
     3661 gctgatgctg catgtgttca ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat
     3721 tgatttcaca aaccgagtct ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg
     3781 ttattccggc cgcggggcaa aaatgcgaat gccgcatatc gtccccctct cagaacagtc
     3841 catcgccatt ctgaaacaga ttaaggatat caccggtaat aatgaactga tcttccccgg
     3901 cgaccataac ccgtataagc caatgtgtga aaacacggtc aataaggcac tgcgggtgat
     3961 gggttacgac acgaaaaagg atatctgcgg tcacggcttc cgggcaatgg catgcagtgc
     4021 gctgatggaa tcgggtttat gggcaaagga cgcagtagaa cgccagatga gtcatcagga
     4081 gcgcaatacc gtgcgcatgg cttatattca taaggcagag cacctggaag cccgcaaagc
     4141 gatgatgcag tggtggtcgg attatctgga agcatgccga gaatcttatg caccgcctta
     4201 tacaattggt aaaaataagt ttatcccata gtatactaac gaatcccaca ctctggcaaa
     4261 ttaccaccac ctctacagga ggtggttcta tatagacttt tatcaacttt atgacgaaaa
     4321 acacct
//
LOCUS       DADLXX010000212         4217 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLXX010000212 DADLXX010000000
VERSION     DADLXX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.217"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..209)
                     /locus_tag="JNX27_003957"
     CDS             complement(<1..209)
                     /locus_tag="JNX27_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7884949.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JNX27_003958"
     tRNA            complement(324..399)
                     /locus_tag="JNX27_003958"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JNX27_003959"
     tRNA            complement(406..480)
                     /locus_tag="JNX27_003959"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JNX27_003960"
     tRNA            complement(597..681)
                     /locus_tag="JNX27_003960"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JNX27_003961"
     tRNA            complement(690..765)
                     /locus_tag="JNX27_003961"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JNX27_003962"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JNX27_003962"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7884950.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JNX27_003963"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JNX27_003963"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7884951.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JNX27_003964"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JNX27_003964"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7884952.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1094 a         1048 c          936 g         1139 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtacgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc acaatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggat
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 cctgacggat agccttt
//
LOCUS       DADLXX010000213         4215 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLXX010000213 DADLXX010000000
VERSION     DADLXX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.218"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            154..768
                     /locus_tag="JNX27_003965"
     CDS             154..768
                     /locus_tag="JNX27_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY7884953.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            768..1097
                     /locus_tag="JNX27_003966"
     CDS             768..1097
                     /locus_tag="JNX27_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY7884954.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            1109..1999
                     /gene="cyoE"
                     /locus_tag="JNX27_003967"
     CDS             1109..1999
                     /gene="cyoE"
                     /locus_tag="JNX27_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY7884955.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FSANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            2184..2495
                     /locus_tag="JNX27_003968"
     CDS             2184..2495
                     /locus_tag="JNX27_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7884956.1"
                     /translation="MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            2479..3021
                     /locus_tag="JNX27_003969"
     CDS             2479..3021
                     /locus_tag="JNX27_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7884957.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(3077..4012)
                     /locus_tag="JNX27_003970"
     CDS             complement(3077..4012)
                     /locus_tag="JNX27_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7884958.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT         1019 a         1002 c         1015 g         1179 t
ORIGIN      
        1 gggcgcttac ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
       61 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
      121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
      181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
      241 tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt
      301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
      361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
      421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
      481 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
      541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
      601 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
      661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
      721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
      781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
      841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
      901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
      961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     1021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     1081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     1141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     1201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     1261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     1321 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     1381 cttgctgggt attgctggct ttatgctgct gtggtttagc gcgaatccgc tggcctgctg
     1441 gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca tgaaacgcca
     1501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgattggcta
     1561 ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     1621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     1681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     1741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     1801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     1861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     1921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     1981 gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc acctttttta
     2041 ttgtctgaga tcagagatac ggctaataac cgtatctctt gacagagtga gcagatgcac
     2101 ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc tacgatttca
     2161 ttcagttaat caaagaagtc ataatgcttg gaaaaacagc cgctctatac gatgtcgata
     2221 aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa aacaaacttc
     2281 gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc gctgctatgg
     2341 aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat caaagctggg
     2401 aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc aaggcgttaa
     2461 tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc catagtcctc
     2521 tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga ttgcggtaac
     2581 cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa tagcgactgt
     2641 gcggctaaag cacttattga cagacaaagt ggtgaactga ttggcatctg tacttttacg
     2701 gcatattcgc tggaaagaca acgcgtttct ggcgtccttc agggttcaca accttcagaa
     2761 attggtgttg tcagattagt catgttgggg ggagcgcgga agtatcaaaa gcggggcttt
     2821 ggtcaggacc tactatgtga tttttttgaa catgtaaaaa aaattcacca ggcattacca
     2881 attaaagggg tttatcttga tgctgaccct gccaccatta acttttatgc tcgtctcggc
     2941 tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt tttggcgatt
     3001 cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt ttgccttagc
     3061 cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc aactccacat
     3121 ccttgccgtt aatagttgcc gttgcggcaa cgattgtggt ccttacagtt gcacaagatt
     3181 ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat gaaccaaata
     3241 ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag ccaaccgttt
     3301 ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaaga ttggccactt
     3361 cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct ttatctaaaa
     3421 aattatcccg catcccagaa cgggtaaccg tgatcacggg taacgtatcc tcaaccgttt
     3481 ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca ccataaatgt
     3541 ggacaccttt atcaaggtat tccttcgctt tcttacaatc tacctcaaca tactgaccat
     3601 tggcgtacat catgcccaac ctggaccatg ctaaattcat accttgctgc gcggcctgat
     3661 taaatagtgc atacgctttg gcataatcca cccgcacctg ctcaccgtcg tagtagatac
     3721 tccccagcgc taacggagct tctctgtcac cttgtttagc ggctaactcg aaccatttaa
     3781 tcgcttcctg aacatcaggt ttggtatcat agcgaggctg ataatattcc cagcctaaca
     3841 ggctctgcgc gtcgatatct cccgattcag ccattttatg cagtgtattt ataacatcgg
     3901 gagcagattt accaccgtat atgctgtcat acaattcatg cgctttctta cttgccacca
     3961 cgcctgatga aactatcagc attaaagaaa ataatattct attaggcaac ataaaatatc
     4021 tcttatttat cttgtacctg aatatgctct tcttttaata aatcacgttg ttttccattc
     4081 agtatcgctg tcgcagattt aatagaataa gtcaaatcat gaaaatcgca taaattacga
     4141 ttatacattg ggatgtctat atagcttttt cttatataac tggaattatt atacatatcc
     4201 acaccgagcg tattc
//
LOCUS       DADLXX010000214         4213 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLXX010000214 DADLXX010000000
VERSION     DADLXX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.219"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..439
                     /gene="tnpA"
                     /locus_tag="JNX27_003971"
                     /pseudo
     CDS             1..439
                     /gene="tnpA"
                     /locus_tag="JNX27_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JNX27_003972"
                     /gene_synonym="qseD"
     CDS             complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JNX27_003972"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7884959.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQEAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTPPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1846..2565
                     /locus_tag="JNX27_003973"
     CDS             1846..2565
                     /locus_tag="JNX27_003973"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7884960.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2631..3929
                     /locus_tag="JNX27_003974"
     CDS             2631..3929
                     /locus_tag="JNX27_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7884961.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          914 c         1037 g         1202 t
ORIGIN      
        1 gcgcacaccc gatggaactg taaatatcac atagtttttt gcgccaaaat accgaagaca
       61 ggtgttctac agagagaagc gtagagcaac aggcagtatt ttgagaaagc tgtgtgagtg
      121 gaaaagcgta cggattctgg aagctgaatg ctgtgcagat catatccata tgcttgtgga
      181 gatcccgccc aaaatgagcg tatccggctt tatgggatat ctgaaaggga aaagcagtct
      241 gatgctttac gagcagtttg gtgatttgaa attcaaatac aggaacaggg agttctggtg
      301 caggggggac tacgtcgata cagtgggtaa gaacacggcg aagatacagg attacataaa
      361 gcaccagctt gaagaggata aaatgggaga gcagttatcg attccctgtc cgggcagccc
      421 gtttacggac cgtaagtaac gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg
      481 gcgcggctgg taagagagcc ttataggcgc atttgaaaaa cctccggcta tgccggagga
      541 tatttattgc acgtcgtcaa ttgcggcgtg ctggctcttc cattgatatc tgctggatta
      601 aatcgtctgg catatgttcc tgtaaaatac gccagaggtt ctcggcggtt ctattcagtc
      661 gggtgttcat tcggtaaata taagcctgaa tggggactgt caatttagca gcatcaaggc
      721 atatcagacg tttggtctgt agttcatcga cgatcgacca gatcggtaac caggcaatgc
      781 cgtatccgtt gagcgccata ttctttaata actcgctcat cgaagagata aatatcgtgc
      841 gcccggtaat acctccaacc tctgccagat gacgattgac caacctgccc atataagatg
      901 tggcggtata gttgagcaaa ggaacctggg gctgagtgat atcgaataca gggtgcccct
      961 gtgcgtcggc ggcacatacc gggtacaact cagattcaaa cagtttcagg cagcaaaaag
     1021 gcgattgcat aagatcttca tcgcgaaacg aaataataaa gtcactcttg ccttcgcgta
     1081 acgtatttac ggcctggaca acatcaatgg cttcaacatg atagacaaat tctccgccat
     1141 aggcggtaag tgaatgcacc agtttgggta atacgctcag ggatagtgag tgcgcggctg
     1201 caattttaat gttgggaacg ccgagtaaac tctggccgtt taactccccc aaattgcatt
     1261 caagctgctg taataagctg cgagtctggg aatgaaagag cttgccctct tcggttaatt
     1321 gtaaaggggg ggtcgtgcga tcgaataaaa caacgccgat cgctgcctcc agcgccttaa
     1381 ttcgtcggct gaatgcgggt tgcgaaagat tacgctcttt tgcctcctga gaaaaatggc
     1441 gacaggcttc cagtgtcagg aaatcgtata accattttgt ctcgatattc ttcattacca
     1501 tggcacctgt attttcaatc caaagacata ataaaacatc ccggcaataa aaataatgtt
     1561 ctcaattagc acaacctggc ctttatcatt cgttttgtgc ataacccctt catttccggt
     1621 attgacgttg atatcacatt tcgccaaaaa aacgaaattt ttccgtcaga taaccagaat
     1681 acatcatcac tatggtgaat aagcggaatg ttccatagct gcatcatgcg tgtcttgcat
     1741 cgccttgtac aaccttgcat ttctcatcgc ccatttcgac tgttaaaaaa gagagcagca
     1801 gttatttacc gcgtattgtc attaacgttt tgagaggcag gtattgtgaa aggtttaatt
     1861 ggtgtactcg gtggaatggg gccggcagct accgtcgacc tgtttaataa atttgtaact
     1921 tttaccgctg cacaatgtga tcaggaacat attcctttaa ttatttcttc gatcccggat
     1981 attcctgacc gtaccgatgc attaatgcat catgggcgtt caccattacc cgttatgcgt
     2041 gattatatgc ataagctgga agatgccggg gcagaatgta ttgttattcc ctgtaatacc
     2101 gcgcattact ggttcaaaga attaaaagat gcttgtcata ttgatatatt aagtatcgta
     2161 gaaactacga tcaatgaagt tcgcgcctgt ggtaaaaccc gaattggctt gttagccacc
     2221 aatgccacgc tatatatggg tttataccaa aaaggaattg agtcgctggg atttacctgt
     2281 gtcagccccg atgccgacgg tcagaaaaaa gtgatggaaa gtatttattc cttgaaagct
     2341 ggcaatatag cgcacgcaca aaagttgatg aatgagcagg ctgaaatatt attttcccgc
     2401 ggtgcagaaa tccttgttct tggttgtaca gaaattccgg ttattctggc gcaagccgtt
     2461 aaagagcagc cttcccgcta tattgactca acggcgtcac tcgttcgtgc cggaataaaa
     2521 tggtacgaaa atcgtgtcgg taaacattat cttttaaccc aataatcatt taaatcgcag
     2581 ccagaaagtt attcgctttt aactgaatta tatttataac ggagaacatt atggtttggc
     2641 tggaaattat cgtagtactt ggtgcaatat tttttggtat tcgccagggg ggaatcggta
     2701 ttggtttatg tggcgggctt gggcttgcca ttctgactct gggatttggt ctgcctatgg
     2761 ggtcgccgcc agtcgacgtg atcctgatta ttatgaccgt tgttgtggcg gcctcagcct
     2821 tacaggccgc aggcggaatg gattatctgg tacgcgtcgc tggtaatttt atgcggcgta
     2881 acccgaaata tatcaacata atcgcgccga ttatcacctg gtctatgacc attatggccg
     2941 gtactgggtt tattgttttt tccacgctgc cggtgattgc tgaagtggcg aaagagtccg
     3001 gtatccgtcc ttcccgcacg cttgccggtt cggttgtcgc ttcgcaggtt gcgatttccg
     3061 gttcgccgat cagcgccgca atggcggcta tgctcactat tatggaggta aacggtgtca
     3121 gttttattca agtgatgtcg gtatgtcttc ccacctcttt tgctgccgcg atggtcgccg
     3181 cttttattgc ttcccgacag ggatgtgagt tgcaggatga cgaggtctac cttgagcgcc
     3241 tgcaaaaagg actggtgcaa aaatacgaga ataatagcag catcaagcca ggtgcgacgc
     3301 tctccgtcgg attgttcctg ctggcgacgg tcgctattgt tattcttgct gccttccccc
     3361 aactgcgtcc gggttttgat atcggtaaac cgatggaaac acgcgatatc attattatct
     3421 gtatgctctc tgccgcctgc ctgatggtgg tgctgtgtaa gacggcgacc gatgccatta
     3481 ttctcacctc cacattccgt gcggggatga gttcgctggc ggtaattctc ggcattgtaa
     3541 ccttaggcac cacttttatt gatgcgcatt taactgagat taaagatatt gctggtgata
     3601 ttttgcaggc gtatccgatg ttgttggcgc tggttctttt tggcacttgc gcgctgctct
     3661 actcgcaagg tgcgaccacg ccgttaatta tcccgcttgc cgtcgcgctt gacgtgccca
     3721 cctgggcaat tctggcgtcc tatgtggccg tcaccggagt atttgtactg ccaacgtatc
     3781 caacatcact ggctgccatg gaatttgata ctaccggcac aactcgtgta ggcaaatatg
     3841 tgctgaatca tccatttatg ctgcctgggt tggggggaat tattgctgga gtggcattag
     3901 gctttgtgat tgcgccaatg attatttagc actatcaaac atcagtatcg atggtttata
     3961 cacaataaag acagatatgg cggaatatag tctgttgtaa gcgccaacaa agaatagggg
     4021 aattccgcca tgtctgaaca tcttcacaat atggtattaa gtgttgtcgt ttaatgattt
     4081 atttcttggt tatgttgtgt attagctgca tctatcgctt tttgatacct gaggtgtact
     4141 ggcaatagcg gacactacca tttgttcttt ttttaagcag tcatctgatg atatttttcc
     4201 ctgaaggctg ccg
//
LOCUS       DADLXX010000215         4202 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLXX010000215 DADLXX010000000
VERSION     DADLXX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.220"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..485)
                     /locus_tag="JNX27_003975"
     CDS             complement(<1..485)
                     /locus_tag="JNX27_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7884962.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
     gene            complement(696..2093)
                     /gene="ccp"
                     /locus_tag="JNX27_003976"
     CDS             complement(696..2093)
                     /gene="ccp"
                     /locus_tag="JNX27_003976"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7884963.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2498..4147
                     /gene="treF"
                     /locus_tag="JNX27_003977"
     CDS             2498..4147
                     /gene="treF"
                     /locus_tag="JNX27_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7884964.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1078 a         1072 c          983 g         1069 t
ORIGIN      
        1 accggaccgc acaccaggtt tggtttatcc gttggtttgc ctgcagcttc catacgcttg
       61 cgccaacgcc atttcatcgc catcccgccg agcatacagg cctcggaaga accaatggtg
      121 ttggtgccaa cggcctgacc atttttcggc gcaggcgcat gccacagatc ggcaaccata
      181 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
      241 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      301 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      361 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      421 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      481 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      541 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      601 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      661 tcgcccaaac attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc
      721 gtgtacaccc cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc
      781 agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg
      841 tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga
      901 cgcaatttat cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt
      961 tccccaaagt taaagtcttt tttcagcacc aacggttcaa aggaacgtcc gccgagaata
     1021 ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt
     1081 tgcgccgtca gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta
     1141 attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc
     1201 ggataaactt cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt
     1261 tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc
     1321 tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc
     1381 gtcggcgcat taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg
     1441 acgccccccg cattcaacgt atggcaatga gcgcatgaaa tggtgctatc agccgataaa
     1501 cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt
     1561 tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa
     1621 tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc
     1681 ttacccgccc agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc
     1741 cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc
     1801 acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc
     1861 gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat
     1921 cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca
     1981 tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag
     2041 caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt
     2101 ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct
     2161 gcgagacaaa gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt
     2221 acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat
     2281 atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc
     2341 gtatttttat aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt
     2401 gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc
     2461 ccgataatta gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa
     2521 ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgaactgg acccgtatga
     2581 attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc
     2641 cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc
     2701 ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct
     2761 ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt
     2821 tgttgaaaat cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca
     2881 aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga
     2941 tcacattccg tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg
     3001 ttttagcgaa acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg
     3061 tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg
     3121 tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc
     3181 gctgatggtg gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca
     3241 ccttaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc
     3301 ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg
     3361 cgacacgccg cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg
     3421 cccgcccaac gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc
     3481 ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc
     3541 catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct
     3601 gaaaggcgag aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc
     3661 ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg
     3721 cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa
     3781 ccatgaacag gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg
     3841 gattttggca agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc
     3901 gttacaatgg atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga
     3961 aatcgcgcgc agctggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat
     4021 cgagaaatac catattgccg atggtgttcc ccgcgaaggc ggcggtggcg agtatccgtt
     4081 gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga
     4141 accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt atcctcttca
     4201 gg
//
LOCUS       DADLXX010000216         4113 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLXX010000216 DADLXX010000000
VERSION     DADLXX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.221"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            244..1199
                     /locus_tag="JNX27_003978"
                     /pseudo
     CDS             244..1199
                     /locus_tag="JNX27_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1196..2101
                     /locus_tag="JNX27_003979"
     CDS             1196..2101
                     /locus_tag="JNX27_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY7884965.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2098..3168
                     /locus_tag="JNX27_003980"
     CDS             2098..3168
                     /locus_tag="JNX27_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY7884966.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3304..3981
                     /locus_tag="JNX27_003981"
     CDS             3304..3981
                     /locus_tag="JNX27_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884967.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1070 a          969 c         1139 g          935 t
ORIGIN      
        1 tcacttatcg atcaactgat aacggaagtc atactgagtt cacactccag agctgcggca
       61 ctggcggttg attctgccgc cgcaaaactg atgcagaatg cagacaatat cagtgaatac
      121 ctgtcgttac accatcaggg gctgatgcaa agtattcagt cactgcaggc gcatatcacc
      181 agcctgcttg aggatatccg ggaaatagca gactgtcagg agcaggtgac cgctgatgtc
      241 agaatggcca tggaggagat cgatgccaga acccgggaat tactgacggg gtctgcacct
      301 cactggaaga ggagctgaat gactatttca gaagcggtaa acgcaaagaa cagcaaatgc
      361 tggaggaaga aaattcagcg cagccccggg agagaaacgc attcgctttc tttcacgata
      421 ttttcggaac cggaaatcag catgacagga tgcgggactt tgatccggac agccccgaaa
      481 taaaattcag tgatcgcagg gaagcccttg aactgatgac gcaaatcgaa tcgaccgtga
      541 ccagcctgca ccgagaggct gaagcacagt tccggcctga gctggagaaa atcgtcagcg
      601 ggattgaaac aggttttcgt ggcacggccc tgtacgccac agaaaacatt gccggtcgca
      661 tcaatacccg cctggaggat gagggcttta ccgtaaaaat cagttttccg gcagtcagtc
      721 agttacaaac ccggctcgcg gtaaaaacaa atctgagtgc gcttatggag gaaagaacgg
      781 agaccgtcac ccgtcgccgt cggcagcgtg gtttatgggg aaagatttgt ggagcgtttg
      841 gcaccagtga ctggggctgg gaaacctata aagaggatgt gagccgcagt gttatcaata
      901 tcaacaccgt caggaaggaa gttatgtcac tgacccgggc atatttcggg gagctgcagg
      961 catccattga gcaggatatt aaccagcccg tccgccagga aatcgatgcc tttttctgcg
     1021 cattcaggga gaaagttgaa caactgcgta acacgctgat tcagagctct gaagatcata
     1081 aacgcgatca gcaggcacag gaacggctta ccaggcgact tcaggcatta aatgaaaggg
     1141 ttcctgagct cattactgac agtaaggcgc tgagggaaga gctggagaca atgctgtgac
     1201 ttcaccattt attcaacaaa ttgctgataa ccgggtatgt caggtactca cctgtcttcc
     1261 tgaaaaattt gtggttgatt tcgcaaacgg aatcgatgtt gcacaggagc atatccgcac
     1321 ggccggagag cgcacgttct tccggcgttt aaaagaggga ctgaccggcg aaggcgctgc
     1381 acggcagaat gccatcaatg cttcggttgc acagggcgtt gaggcgtccc tgcgctggct
     1441 gacggagctg acaacgtcac ttgccaccac aaactacgcg attacccggg taaacgacag
     1501 ggtcagttca ctggtcagtg atactgccag gttggcgcat tattccgcag atacacggga
     1561 gcagttactc atcctggccg atcaggttca ccataaactg aatcatcttg aagaaaaact
     1621 ccaccgtgtt gaccaggttc agcgggcgca gttacatctt gagcagatat tctcatggtg
     1681 gagcgccggg cgatacgcgt cattttcccc tgccggacgt tgttatgtgg cacttgaaga
     1741 gcttcgctgg ggcgcgtttg gtgatgtgat acgtcagggc gaaacaggcc aggttaacca
     1801 gctactggat attctcagac ataaagcatt aacgcagatg gcacaggaga gtggcggtag
     1861 tgcaaccgtg cgtctgaaca ctctggactg gcttggtggt cagggccggg agcaggcaga
     1921 taacgagtgg catgacgcga ttaactggct gggagactgg tgcagtgaag agcagcatcc
     1981 ggtgatctgg tcaaccacgc aggctgcaga acacttaccg gttcgcatgc ctcgcctctg
     2041 ctctgcagaa cgcctctctg aaagtatggt cgatgaaata tttcagaagg gggctgcatg
     2101 agtacagaaa tgaaaacggg gctggtgctg tccggtggcg gggcggtggg cgcttatcag
     2161 gcgggagtgg ttaaggcact ggcagagtgt gatacacaga tcagcatggt ttcaggggcc
     2221 agcattggcg cattcaatgg tgccattatc gcggcctctc ccgatctgtc agaagctgcc
     2281 gtacgcctgg aagcgctctg ggatcatctg gggaataatc aggtgctgtc ggtaaaccga
     2341 ttggtttact tttcattgct gaaaaaattg ttccaggcaa tgaacctctg ccagatcccc
     2401 ggacgtgcag gagcactgct tacgacgctt cttcgccata tatcgatact caacgggttt
     2461 gacaatctga tggctcagcc gttgttgtca gatgagcccc tgacagcgct gttggatcat
     2521 tatcttgata ctgatgctct ggcagacggg ctaccgctgt atgtgtcgct gtaccccaca
     2581 gaagggggca tgcaggatat tattgactgc attcgtgctg aactgggtgc cggaaccacg
     2641 aaaaacgccg tttttcagca tatccagagc ctgccccgcg gacagcagaa agaggctctg
     2701 cttgcgtcag ccgcgctgcc cctgctgttc cgtccccgtg aggttcaggg gacaatgttc
     2761 ggtgatggtg gtatgggagg atggcgaaat atgcagggaa atacccctgt gacgcctctg
     2821 gtcgatgccg gatgcaatat ggtgattgtg acgcatctga gtgacggttc tttatgggat
     2881 cgccaggctt ttccggacac cacaatcctt gagatccgtc cccggaaaag gctgaaatat
     2941 gcaggtgatg gcgacaacag cggcggtctg ctcagtttca cgttggcaca taccgacgcc
     3001 tggcgtcagc agggctatga agacacgatg ctgacgatgg agcatatccg gaaaccgctg
     3061 gcagcacgtc aggtactgtc ccggtcagaa acggtgttgc agaaaagtct ggagataacg
     3121 gaagaggcag atttggcact gagaaacgcg atggcccgga ttaaataaag acgctccaaa
     3181 gaaacgccac cgcctgtggt ggtgtttttc ctagctggtg ttattgaaaa tattttcaga
     3241 tatatattac ctgagagaaa gttttacccc tcacctgtca attcactcac tggaaattta
     3301 attatgcaat tacctgtaaa gttattaatg agccttatat ctctggtcag cgttattgca
     3361 cgtgccggga aatataaaaa ttatatccgg gatgaaataa aatactggcg atatacatca
     3421 tacaagggtg ggaaatttcc ggaaggtttc actgatgaga aattttccag cgccatttac
     3481 aacggaagaa tatttacaat gaaacgttta cataccctga tgttatttct ggcggttctg
     3541 tttactggct ttaacgtgga agcagcgagc gtgaaacaag cgctcagctg cgacccaaac
     3601 gcccgggctg aacaacctgg agcgtgtcca acaacgtacg agttgtacga aggtgacgct
     3661 gcctacaaag ctgcgcttga caaagcatta aaaccggtcg gactgagcgg catgttcggt
     3721 aaaggcgggt atatggatgg ccctggcgga aacgtaacgc cagtaaccat taacggtaca
     3781 gtctggctcc agggcgacgg ttgcaaagcc aatacctgcg gctgggactt tatcgtaaca
     3841 ctctataacc caaaaaccca tgaagtcgtt ggctaccgct actttggttt agatgacccg
     3901 gcctacctgg tttggttcgg cgaaattggc gtgcatgaat tcgcgtatct ggtgaaaaac
     3961 tacgtagctg cggttaacta atttaaggag tctgtcatga aaacttttat caaaacttta
     4021 ctcgttgctg taactattct gttctctgtc ttcgctacgg cgaaacaagt aaaactgcca
     4081 aacaacatca aatacgttaa tactacagag gcg
//
LOCUS       DADLXX010000217         4089 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLXX010000217 DADLXX010000000
VERSION     DADLXX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.222"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            138..465
                     /locus_tag="JNX27_003982"
                     /pseudo
     CDS             138..465
                     /locus_tag="JNX27_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(495..3407)
                     /locus_tag="JNX27_003983"
     CDS             complement(495..3407)
                     /locus_tag="JNX27_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884968.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGAAPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3410..3526
                     /locus_tag="JNX27_003984"
     CDS             3410..3526
                     /locus_tag="JNX27_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7884969.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3890..>4089
                     /locus_tag="JNX27_003985"
     CDS             3890..>4089
                     /locus_tag="JNX27_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HAY7884970.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPR"
BASE COUNT         1037 a         1060 c          990 g         1002 t
ORIGIN      
        1 ctttcagtaa gtacgacagg tactgtggag gcacttccgt aaagaagggc atcaagctcc
       61 tgctccgaca tacctgcagg caaaggccat gaaagccctg atttccggaa gcgagcgaac
      121 agtccacaaa ctgttgaacg tggtgtcagc ggcggacagg gataagccct cgtcagcagt
      181 tccgtcagca ctgcgacagc gttctttacc cggtcaaaac agcgttgcgg tgcaccacgc
      241 ctgacggatg aactgcgtgc tcagggttac cactttaacg taaaaaccgt ggcggcaagc
      301 ctgcgccgtc aggggctgag ggcaaaggcc tcccggaagt tcagcccggt cagttaccgc
      361 gcacatggcc tgcccgtgtc agaaaatctg ctggagcagg atttttacgc cagtggtccg
      421 aaccagaagt gggcgggaga catcacgtac ttacgtacag atgaagttcg gttacatcct
      481 gtcagcacgg aacctcacgc tttttgagcg gcacgacgga tcccccccac ccagtatccg
      541 acagactttt cactgcgttc gtaaaaaccc ttcttccctc ccagcatatc cactaattcc
      601 ggtgacatat cctgcagata agtctgcaaa tcatgcccca gatcttccgg tgactgcaac
      661 tcataaaccg cgcgccacac atcaccctgc cttattttaa gccccgggtt aagaatatac
      721 tgtccgtggt tcagcctcag aaaaagcttt tttccataag gatttttgct gttaacttca
      781 tgttgagaga gctggctgct gatataactc cttcgtttgc ggcgttccgg cagcaatgct
      841 tcaggaagat gaaccacaca ctctgccagt cgtgccgcac tgaaggcttc cgaaaaaaac
      901 agagcacttc ccagatgcgt gtggaacagc gcctgcatca ggatcaccag aaacattgcc
      961 atctgccggt tgtccagttt gatgaggcgt ccttccacct gcagtgacag actttcaggt
     1021 ttcagcaggg tatacaactg cgcacttttc tgttgcgcat aacggggcgt actgaccgcc
     1081 gctgacagaa cttgatgata agccgccagc ccgtcattac cggtcagcaa gggagctgca
     1141 ccacgctcca gcagtaactg aaccagtgca atattacctg cccgggctgc tgacatcagc
     1201 ggagtaagat taaacggcga acggtgatca acaccataac gttcaatatc ccgcaacacg
     1261 gcaaccgggt tattcattga gtatggccgg aaatacttca gctccagtaa ctgcagagtc
     1321 ttcgttctgg gctgccttgt gggcttaaaa tcttcccggt acagtgcatt atacagcggg
     1381 aacagggaat aaatcagact gtactccagc gcctgcagtt gtatggtttt atcggctgta
     1441 cctgcgagga tatgctgttt ccactgtcgt gcatcctctg cggtgataat ctgccatggc
     1501 atctcagatg tctgcaggat ccggctacgg atggtgtccg cctgctcctg cttcccctgt
     1561 ttttcaagca gatgtgcggt tttctgccac tcatcacggc tggaaatctc ttcctgcaga
     1621 ttcagaggct cttcctgatg cgtgagcgca agcagactcc acagaggatg ctccacctgc
     1681 tgttcaacaa gatacacatt gtgtgtagca cgggtgagcg caacatacag cgcgttggta
     1741 aaaaatttat agatttccgc tgaacgatcc tgcctgtccc gcggacggga ataacgggcc
     1801 tcgccctcaa gatcagccgg tgtaagaccc tcgcaaatat catcaaatgc ctgccgggca
     1861 gcgcttacta tattgtaaag gatcaccgtt tcgtattcca gccccttaac ctcctgaacc
     1921 gaaaagacca gcggcgtact gaaccagcat cgcgcctgtg ctttctgttc cggatgcatc
     1981 acaatgaccg ctacacgatt cgaaagtgag gttttggcat tcagttcctg ctttgtctct
     2041 tccctgtcgt ccagcaaccg gatgctccct tctgcctgac cgcagcttct gacaaaatgg
     2101 tgactttccc gatcaatagc actaaagcgg acctgtttga gtctcagcaa ccggttagcc
     2161 agtgcagtga cgtggtgccc gttacggtaa ttcgcctgca ggatattaac ggttgtgtcg
     2221 tttccctgtt gctgccggaa aaagagactt ttgagcgaac tccatgagaa aaagctcgga
     2281 tgaactatct gattggcatc accacagagt aaaaactgac cgggatgacg tagcgttttc
     2341 agtaccagtt gcagttgaac aggcgtcata tcctgaactt catcaacaaa aatgacatca
     2401 taacggggag ttgccaacga aagatactga tgactgagca gattactgtc atactgatgc
     2461 gactgtttca gccatgcgat ataacgttca aacaggacat agaccgtcgg gcggtcctcc
     2521 atgccataga gcgactgccg tatccccagt gacagatacg cctcccggga cagcggccca
     2581 ttcccggagg caaccgcacc gatcacgccc ctgaactcct catacagggt atgggcagcc
     2641 cccagtgcgg cgatagccct gttgcggggc agccagtcgc tgaacgcact gaaagtcacc
     2701 tccctgcctt ccggaatgcg cagagtttcc agaaattccg tcagcgaaag aaagtcaata
     2761 ttctgtacct cactcccttc ccctgaagcg tcatagagag tacgcgcttt ttccaccaga
     2821 aatgaggaaa ggctgagata cagtatatca ccagcagcct gcttcatttt ttccagtacc
     2881 agcgccgtct taccacttcc cgccgagcca acaatcacca gcggtagcgg gtgttcgtac
     2941 agtgcctgct gatcatcatc aaaggacagc attttatcca ggcggtggaa acgcccatga
     3001 gacggattga tatacgtcag cgcttcagtc gcgatatcaa caggatccgg cactggttgt
     3061 tgttgtaagc gcccttcatc aatcaccacg tttctgcgaa gaaaacgtga agtgttatag
     3121 gcatgattgc gaatatattc cagaacaagg cagatagttt caccgtggta gcgatacagc
     3181 gaaaacagta atcggtcccg gatactgagc cgggcgcggt aaagattggt gtcaatttta
     3241 cgaacatcag cctgaacaaa gttatcggtg gcaatagcct gtgcaaattt tttataaccc
     3301 gggatcgtgt caggattcag agtatgataa agacagattt tcatggctgc cggtttccgt
     3361 cgtgcaaaaa acatgctgat tttacgaagg aatgccttac tgaacaacct tgcccggaat
     3421 accccccgaa atcagatgga acaatcagaa aaatgctctg cagttatgga ataaagaagg
     3481 tggggtggta cgccgggagg gcgatccgct ggaaatggcg cagtagctgc aggcctgtca
     3541 tgatgccgga atagaagttc gtgtacaggt taatgaaagc gtcaccccgt aaataaactc
     3601 tgcgctgaaa ttcctttttt tcattggagc acctgtgttg ttctgaggtg aacatatcac
     3661 ctctgttcag gtagccaaat tcagtgtgcc acctcaaggt tcttagtgag tcgtttttaa
     3721 aagatatgat aatatatcat tgtttatgaa ttgttgatgt ttaatttagt aaattatatg
     3781 aaatatgcca tgggtttgct atgttattca tccagtatct ggaaataaaa tgcaccagct
     3841 ttattgggtt ttccttttgt ctctgcttac taggtgccag aaactttaaa tgttaaacga
     3901 agggggcatt ttgttcaaag ccaatcatgt tccggtatta atgtatcatc atgtcagtca
     3961 ttgccccgga ctggttacac tttctcccgt gacattccgg aagcaaataa aatggttggc
     4021 tgaaaacaac tggaaaacac tgtcatcaga tgaactggag tttttttatc ggggcggaaa
     4081 acttccccg
//
LOCUS       DADLXX010000218         4077 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLXX010000218 DADLXX010000000
VERSION     DADLXX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.223"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..78)
                     /locus_tag="JNX27_003986"
     CDS             complement(<1..78)
                     /locus_tag="JNX27_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7884971.1"
                     /translation="MTDITANVIVSMSSQLFTMARSFKAV"
     gene            complement(210..2051)
                     /locus_tag="JNX27_003987"
     CDS             complement(210..2051)
                     /locus_tag="JNX27_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7884972.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2051..3457)
                     /locus_tag="JNX27_003988"
     CDS             complement(2051..3457)
                     /locus_tag="JNX27_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7884973.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQENQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVGPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(3467..>4077)
                     /locus_tag="JNX27_003989"
     CDS             complement(3467..>4077)
                     /locus_tag="JNX27_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7884974.1"
                     /translation="NTLQETRGGSQKYAADLQNQQWQTIMKNLTPFTPLAEQYVNQLQ
                     NLSSLEGQGQALNQYYNSQQYKDLAGQARYQSLAAAEATGGLGSTATSNQLATIAPTL
                     GQSWLSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSL
                     QTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          930 a         1044 c          920 g         1183 t
ORIGIN      
        1 tacggcttta aaagaacgag ccatagtgaa gagttgcgaa gacatcgata cgatcacatt
       61 ggctgtaatg tctgtcattt aatttgctcc agatacaatg aatcgccgca gcgtggctac
      121 ggtgaatttt gggcataaaa aaacccagcc gaagctgggt agttgcgttg gttatctgtc
      181 agtagttatg tgcttattta attatttatc tattgttctg ctgtaatttg gcttgaaagt
      241 atagggcgta tcgcatttgc agcattattt aacgctcttt cataagctgc cgttcctgct
      301 ttggtgtttg ccaaacgaag aagcatattc cttgcggctt tggactcata caaacgcatc
      361 attgcaccaa aaccagcctc aagccccatt gatacaccaa gagtcgcagt tgcgccaatc
      421 gtccttatcc tgttggcttg cgattgcccc gtctgagtta ctacatttgc ggtgtctgac
      481 cttgctgttt gctgtagaac ttcatgaaga gcatcaagct ctttcatgtg ctttccagaa
      541 aaaatagtgt tgtaaatttc accgcctgac tgagatttca gcttattaac ttcagtgatg
      601 aacttggctg gagagtcacc ggccttttcc gctattttgc tgacgtaagc tgcacgcata
      661 gcatctttcc ctttatcatc cagtgcgctc cagattcgtt tcacgtcaga tggttttctg
      721 cttaatacaa cggtatttat aagttcagga ctggcttcac tgcttgcctt gttgagcttg
      781 ttagcaatgt ttttattaag caccttatta taaacgtttg cataatcgga atttgcttta
      841 aggtattttg ctgcgtctga tgcaccgagg tttttagcaa ctgcgttacg aaggtctttt
      901 gacattgcat tctctaccat attggtagct gcttttgcct ggttggggaa gaccatagca
      961 tctccctgaa cattagatct aaatgctgtt ctgtgctgac gcaagagatc aaacgtaaca
     1021 tccaaatcag ttgcagggtt tgctaattct tcacgtaggt tacgcaagga tgtaagcagg
     1081 ctttgattgg cagacgtccc aagccgttcc tgtcttgcga tcgctgtatt cagagcattc
     1141 atggtatttg tggtatcaac tgcggcatta cccattttat tggtgacgtc attgataaca
     1201 gcgccagcgg catccttccg cccccttaac gtggtggtaa gagatttcac cacatcatca
     1261 gggttgtact caccaaaacg gtcaaaataa ttacttacca gcttactacg cgttgcatat
     1321 tgctccgctc gctttgagcc tgtcccgagc aaagccccct cggcatcctg agttaggccg
     1381 cgagtgaaag catttttcgg cgggataaca tcagatgtca ttggtgtcac gcccatcgat
     1441 tctgatgtgg caattttctt cgccacttct ggcgcaatat caccttttat agccgttatt
     1501 ccacgcccta ttccctttgc tgctgcggaa agaacaccct gagcggcaag gttaactccg
     1561 gcatttttag ctgcattttg tgcgaaatcg cctttctgat ttgcggcctc tgccagtgat
     1621 ccaatagcca tgcttcctgc cgttccaact cctggaacta aatacccacc aattgtttca
     1681 ccggcttgcg cgtaggggtc tgtcggtctg tctactggac gataaacatc atccaaaacc
     1741 ttgggtccac caagcccctg actgattgca ttaatcagac ttgcgccgcc ctgcaatacg
     1801 tcaaatggta tgtttaccag accacgacca gcctgttctg caatttgccc tgcactttga
     1861 ccaccagtga gccaatcgcc agcttgttgc atcaatgatg gttcttcccg tgttggtgca
     1921 ttattggcct gattaactgt ttgttgctga acagcctgac cagcaaaata ctcatcaatg
     1981 gcggtgccaa tatcttcggt gctcgtacca tcaggaaagg taaatgtctt accgtttgca
     2041 gttactttca tcattccacc gtaaattgaa tgcctgattt tgaggtatat gatccaacct
     2101 gattccgtgg ttctcctgaa ggtgtcgaat cttgtgctgg cgctgcgtca gtattcattg
     2161 acatataccg cttaacggca ctccccaatg attcaccttt tttaacatcc aaccccaata
     2221 tctgaccgcc attacgcgat tgtccagggt tgccattcgc gctcatccac tcggctttaa
     2281 actcattaaa ctgcgcgttt cgtcgctcaa ggtttgccat tgcatcaagc catcttgcga
     2341 ccgtctcagg gttatccatg tcagttggcg caccctgtcg aacgatctca acgtctttat
     2401 ccgttgctgg gccgggaggt aggaatttaa gaacctgact gttaacaagg gcattttggc
     2461 gaatgcgcaa atcacgcaat gttgtatcgc ttccggtaag ttttgcgaac atgttctgtg
     2521 cgttaccgaa caaacctgtc gttggttttt ctgctctgaa ctgttgagca agcgcactca
     2581 tagaattggc tgagtttgat gatgctgtag cattgtttac agccgtctcg atgccttttt
     2641 ccatgtttac tgacagctta ggtgcttcgc taatcaactg ctgagccttt tcctgcgctt
     2701 gctgcatctt aaacccgaac tcttgctgat ccagagccaa gcgttgtgct gcgatattgt
     2761 gcccagtcat tgctgactga taggaaaggt tttgccctct cgcctgaagt gcttctccag
     2821 cctgattgct gcggattgtc tctgccagcc tgcctcggtc aatttcacga ccagccatct
     2881 tgtcctgaac attgaagtaa tcaatcggac caagagcagc cattccaagg tgatcaacaa
     2941 actcaccaaa tcctgaagga ttctgctgat acatctgagc aacgctgtta gggccaacac
     3001 cgacgcgcgc cagctcattg gcgttgtttt gcagccatga ttgcattgct tctggagacg
     3061 aggccgcaag gcgtgcgcca gccgctaagg tgccgataga attacgctgg tcttcatcaa
     3121 tgaatcccat gcctttacga acggattcaa tctggtctgg atattgagta gccaactgac
     3181 gcaaagcacc gcgatcacca gacgcataag cattagcgta cgcctgctga aattctttct
     3241 gccgctgagc ctgattttcc tgctgaaaca cccctgcaat acctgaaagg ccttgcaaag
     3301 cagtcagccc aacattgtta gcgcctgaac gctcaatatc attgttctgc ctgataagct
     3361 gaagcgtatt gccgatgtca tttacgctcg gagcgtttga gttgacgccg ccgataccag
     3421 ctaacaatcc gccatttgat ccttgccaag tagccatgat taccccttaa aacaacgagc
     3481 caagcaatcc gataccagca ccaatgccag cgccccaagg tgttgatgtt cccaaaaggc
     3541 tggcaagacc tgcaccggca atcgcaccag atgttccgcc actaattgca gtctgaagac
     3601 ttgatggttt attggcatta gcagcggcaa gagctgcgct ttgctgtgca atgctgctca
     3661 tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg cagcgcacca agcccaacgt
     3721 ttgccagatt gttgtaattg ctcatctggt ttgataacca agactgaccg agtgtcggcg
     3781 cgatcgtagc cagttgattg cttgtggctg tcgaaccaag tccacccgtc gcctccgcag
     3841 cagcaagact ctggtaacga gcctgacctg caaggtcttt atactgctga gagttgtaat
     3901 actgattaag tgcctgcccc tgaccttcta aactggaaag gttctgaagc tggttaacat
     3961 actgctccgc aagaggcgtg aacggagtaa ggtttttcat gatcgtctgc cactgctgat
     4021 tttgcaggtc tgcggcatac ttctgtgaac cgccccgggt ttcctggaga gtgtttt
//
LOCUS       DADLXX010000219         4069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.224, whole genome shotgun sequence.
ACCESSION   DADLXX010000219 DADLXX010000000
VERSION     DADLXX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.224"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1587
                     /locus_tag="JNX27_003990"
     CDS             <1..1587
                     /locus_tag="JNX27_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer nickase/helicase TraI"
                     /protein_id="HAY7884975.1"
                     /translation="IVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDG
                     ELRRLSTWENNPDALALVDNVYHRIAGIGKDDGLITLEDAEGNTRLISPREAVAEGVT
                     LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQE
                     RAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVY
                     TDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMSAQRLFSTARELRDVAAGRAVLRQA
                     GLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGEGR
                     VKGSGDAQFVALQGSRNGEGLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
                     GGRVWGDIPDNSVRPGAGNGEPVTAEVLAQQQAEEAIRRETERRADEIVRKMAGNKPD
                     LPDGKTEQAVREIAGQERDRTATSERETALPESVLRESQREQEAVREVAQENLLQERL
                     QQMERDMVRDLQKEKTLGGD"
     gene            1607..2353
                     /gene="traX"
                     /locus_tag="JNX27_003991"
     CDS             1607..2353
                     /gene="traX"
                     /locus_tag="JNX27_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7884976.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            2408..2968
                     /locus_tag="JNX27_003992"
     CDS             2408..2968
                     /locus_tag="JNX27_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7884977.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3100..3312
                     /locus_tag="JNX27_003993"
     CDS             3100..3312
                     /locus_tag="JNX27_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7884978.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            3546..4019
                     /locus_tag="JNX27_003994"
     CDS             3546..4019
                     /locus_tag="JNX27_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7884979.1"
                     /translation="MENNMRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSR
                     KAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDG
                     MNVNQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
BASE COUNT          963 a          971 c         1309 g          826 t
ORIGIN      
        1 attgtcacgc acctgaatga ggaccggcgc gtactgaaca gcatgattca tgatgcacgg
       61 gaaaaggccg gtgagctggg gaaagagcag gtcatggtgc ctgtcctgaa cacagcgaat
      121 atacgcgacg gagagctgcg tcgtctctcc acctgggaga ataacccgga tgcccttgcc
      181 ctggtggata atgtgtatca ccggattgcc ggtatcggca aggatgacgg gctgataacc
      241 ctggaggatg cggaaggtaa cacgcggctg atttcgcccc gggaggcggt ggctgaaggc
      301 gtcacactgt acaccccgga caccatccgg gtggggaccg gcgaccggat gcgcttcacg
      361 aagagtgacc gggagcgcgg ttatgtggcc aacagcgtct ggacggtgac agcggtttct
      421 ggtgacagtg tcacgctctc ggacggccag cagacccggg tgattcgccc cggtcaggag
      481 cgggcagagc aacatattga cctggcctat gccatcaccg cccacggtgc gcagggggcg
      541 agtgaaacct ttgccatcgc gcttgaaggc acggaaggta accggaaact gatggccggc
      601 tttgagtcag cctacgtggc tctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat
      661 aaccgtcagg gctggacgga tgccattaac aatgccgtac agaaaggaac tgcccacgat
      721 gtgtttgagc cgaaaccgga ccgggaggtc atgagtgcgc agcggctgtt cagtacggcg
      781 cgggagctgc gggacgtggc agctggtcgt gccgttctcc gtcaggcggg gcttgccggg
      841 ggagacagtc ctgcacggtt tattgcaccg ggccgcaaat atccgcagcc gtatgtggca
      901 ctgccggcgt ttgaccgtaa cggcaggtcc gccggtatct ggctgaaccc actgaccacg
      961 gatgacggaa acgggctgcg gggattcagc ggtgaaggac gggtgaaagg cagcggggat
     1021 gcgcagttcg tggccctgca gggcagccgt aacggagaag gcctgctggc tgataatatg
     1081 caggatggcg tccggattgc ccgtgataat cccgacagcg gtgtggtggt gagaattgcc
     1141 ggtgaaggtc gtccgtggaa tcccggtgcc atcaccggtg gtcgcgtgtg gggggacatc
     1201 ccggacaaca gcgtccggcc gggggccgga aatggcgaac cggtcacggc agaggtactg
     1261 gcacagcagc aggcagaaga ggccatccgc cgtgaaacgg aacgccgtgc agatgaaatt
     1321 gtccgtaaaa tggcagggaa caaacctgac ctgccggatg gcaaaacaga gcaggctgtc
     1381 agggagattg ccgggcagga acgtgaccgg actgccactt ctgaacggga aaccgcactg
     1441 ccggagagtg tgctgcgtga atcacaacgg gagcaggaag cggtccggga ggttgcccag
     1501 gaaaatctgc tgcaggagcg tctgcagcag atggagcggg atatggttcg tgacctgcag
     1561 aaagagaaaa ccctgggcgg agactgatac aggaagataa acgctgatga caacggataa
     1621 cacgaacacg acacgtaacg attcactggc tgcacggacc gatacctggt tgcagtcatt
     1681 tctggtctgg tcacccggac agcgggatat catcaaaacg gtggcactgg tgctgatggt
     1741 actggaccac atcaatctga tattccagct gaagcaggaa tggatgtttc tggccgggcg
     1801 aggagccttt ccgctgtttg ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat
     1861 ccggcaaccg gccattaacc ggctgtgggg atgggggatt attgcccagt tcgcatatta
     1921 tctggcaggc tttccctggt atgaggggaa tatcctgttt gcctttgcag tggcggcaca
     1981 ggtgctgacg tggtgtgaaa cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat
     2041 ggccctgtgg gggcctttgt ccggcaccag ttacggcatt gccgggctgc tgatgctggc
     2101 agtcagccac cggctgtacc gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg
     2161 tctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggctgg
     2221 tcttgtgatg acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat cattaccccg
     2281 tttctggccc ggggattttt tcccggtatt ctacgcctgt catctggctg tgctgggcgt
     2341 tctggcgctg tgacgggtgt ggtatctttg accgtaacag gatgattcgt cagaggcaga
     2401 acacagcatg acagagcaga agcgaccggt actgacactg aagcgaaaaa cggaagggga
     2461 gacgcctgtc cgcaggcgga aaaccatcat caatgtcacc acgccaccaa agtggaaggt
     2521 gaaaaagcag aagctggccg agaaagcagc ccgggaagcg gagctggcgg caaaaaaagc
     2581 gcaggccaga caggcgctgt ccatttatct gaacctgccc tcgctggatg aggccgtgaa
     2641 caccctgaaa ccctggtggc cgggattatt tgacggtgaa acgccccggc ttctggcctg
     2701 cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa
     2761 aaaactgcgc agggcgctga aggccatcac ccgttcagaa agctatctgt gtgccatgaa
     2821 agccggtgcc tgccggtatg atacggaagg gtatgtgacg gagcatattt ctcaggagga
     2881 ggaagcgtat gcggcagcgc gtctggataa aatccgccgc cagaacagga taaaggcaga
     2941 gcttcaggcc gtgcttgatg agaaataaaa aaaagcctcc atcagagagg aggcagggaa
     3001 ataaggctta aaaggaaata acttccgtac ccgaaaggaa gccaattcag tagattattt
     3061 aacaccataa tactgacggg ttaaagagga gtgataaaga tgaatggatt cagaaacagt
     3121 tcacggaacg gtcaggtctg gcgctggcag cgtgccggaa gccgggccgt tattctggag
     3181 gtcagtggac gctggatgga agcggcagaa gcatggcgac gggctgccgg tgttgcgccc
     3241 cggacagact ggcagcagtt tgcccgaaaa agggctgaac actgccaccg gcgcagccgg
     3301 ggaagggtgt aacgaaaaaa gcagccttca ttactccagg gattcagaag gctgaataat
     3361 gcatgagatt tgcttttttt aatatccggt cgggcagtat gaccggacag ttctgagtag
     3421 cacagagttt tttccgtatt aagtcgtgat tttatatttt gtgattaatt tcacaaaata
     3481 aggtgttatt cagtgtgtgc tgcgatattc aggattacct gaaatacagg tgccatttat
     3541 ctgatatgga gaataacatg aggaaatata ttccactggt attatttatc ttttcatggc
     3601 cggtattgtg tgcagatatt catggacggg ttgttcgtgt actggacggt gataccatcg
     3661 aggtcatgga ctcacggaag gcagtgcgga tcaggctggt aaatattgat gcaccggaaa
     3721 agaaacagga ttatggccgg tggtcaacgg acatgatgaa atcactggtt gcaggaaaaa
     3781 cggttaccgt cacatatttt cagcgagatc gttatggtcg tattctcggg caggtttatg
     3841 caccagacgg aatgaatgtt aatcagttta tggtccgggc cggggctgcc tgggtatatg
     3901 agcagtacaa cacagatccc gtgctgcctg tccttcagaa cgaagccaga caacaaaaac
     3961 gtgggctctg gtcagatgct gatccggttc ccccctggat atggagacac cgtaaataaa
     4021 gcgatggcaa tcttatcatt catctgttct cagcgggaat aaaaatggc
//
LOCUS       DADLXX010000220         4012 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLXX010000220 DADLXX010000000
VERSION     DADLXX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.225"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(249..1607)
                     /gene="plaP"
                     /locus_tag="JNX27_003995"
     CDS             complement(249..1607)
                     /gene="plaP"
                     /locus_tag="JNX27_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7884980.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGSIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1597..1677)
                     /gene="yoeI"
                     /locus_tag="JNX27_003996"
     CDS             complement(1597..1677)
                     /gene="yoeI"
                     /locus_tag="JNX27_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7884981.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1875..2804)
                     /locus_tag="JNX27_003997"
     CDS             complement(1875..2804)
                     /locus_tag="JNX27_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7884982.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2850..3674)
                     /locus_tag="JNX27_003998"
     CDS             complement(2850..3674)
                     /locus_tag="JNX27_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7884983.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3757..4011)
                     /gene="yoeB"
                     /locus_tag="JNX27_003999"
     CDS             complement(3757..4011)
                     /gene="yoeB"
                     /locus_tag="JNX27_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY7884984.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1093 a         1039 c          935 g          945 t
ORIGIN      
        1 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
       61 ttgaaaacat aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg
      121 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc
      181 gataaaaaag aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga
      241 aaagatgatt atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg
      301 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      361 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      421 tggaagtgat ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc
      481 gacaggttaa caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
      541 accaggtcga agttgattgc cagcagggca atcgagccaa ccaggatgat gttcatcgcc
      601 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga
      661 cccattacgt acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg
      721 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
      781 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
      841 aggaagtaag ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca
      901 cgcgggatca cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag
      961 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1021 ccagaccaga acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca
     1081 taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg
     1141 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc
     1201 gccaccacaa acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc
     1261 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1321 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1381 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1441 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac
     1501 agcgtcatcg gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt
     1561 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt
     1621 tacggtgata accgtcgcgt aagcaaaaaa tttgccccat ttttttggat tcctcagcga
     1681 caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct cttggtatga
     1741 atgattggtt tgaagcaaaa aataaccgac gctaattaaa cgtcggtttt tagtcatttt
     1801 ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga gaggcttgtc
     1861 ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata aacaccggcg
     1921 atcgcactat ttgctaaaat ttcatctcgc caccagccgc tggcaagccc tgcggtctgt
     1981 tcattccagc caatccagac aggttcaaag agagtttggg cgaccacttt cttctcgatt
     2041 aacgcgccac tatcgagaaa acgttgcgcc agataccgtg gtaaatagcc gcagccaagg
     2101 ccgctaattt gcagctccag tttggtttta aaatcgaaaa cggtaatggc ttcttgttcg
     2161 tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tgtcccccac cacaatcgcc
     2221 cgatatcgtt taatgatacg acggtttaac ggttcttctt cctgggctaa cggatgatga
     2281 ggcgcgaccg caaagacttg ttcaagatcg cccaaccgcg agaaaccaaa ctcactggaa
     2341 gatggtggct catgcatcgc gccaacgata atatccgctc gcccctgagt cagggcatcc
     2401 caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg atgttgataa
     2461 aaagcttcaa tgagaggcgc aagcagagaa aaaggaaacg tgtcgtccac gccaatcacc
     2521 agctcgtttt cccagccttc atgcagtttg atcgcctgct tttccagttc tcgcacggta
     2581 tgcagaactt cccgcccttt ctctaataac atttttccgg tgcgggtgaa tttagcgcgg
     2641 tgtccgctac gatcaagcaa ttgaatattt aggtcgcttt ccagcttgtg aacggtgtaa
     2701 ctcagagcgg acggcgtctt gtagagtttg gctgacgccg ccgcaaaact gccctctttt
     2761 tctatggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc ccccttgcag
     2821 tgcatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag cggatcggga
     2881 tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat cttacctttg
     2941 ccgctgtcca gactatttct gaactgcgga ggttgcagcc ccagtaaacg ggccatctgc
     3001 ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat gtgtccgcct
     3061 ttaggtgcct gtaacaacag agtgatagcg ccaataacat cttctaaatg gactaaatta
     3121 acaccatgtt caccatcagg ggcggttttt ccggcaaaga agcgtcccgg atgacgtccc
     3181 ggtccgacca ggcccgcaag acgcagaata tcgaccgaag taccgggtaa attgtgcagc
     3241 cagtcttcga gttcttctaa tactcgtcca ctgttggtta ccggattacg cggggtagtt
     3301 tctttaaccg tgccttgcgc gtcgccatag acagatgttg agctggtaaa aataattcgg
     3361 ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa atagaactca
     3421 tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcatcggc atccataagg
     3481 gcatccagat cgtcagaatc gcaaactaac tcaggctcca tgcgaagcag atagctatca
     3541 atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc ggtgacttgc
     3601 cagcctcgcg ctgaaagtga catcgccagc ggcataccta accaccctaa cccgacaatt
     3661 gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct acgctagcgt
     3721 atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac gacatgctgc
     3781 aatgagcagt gaatcatcag taaccgcgta taccagacgg tgctcctctg taatgcgtcg
     3841 ggaccagaaa cctgacaaat tatgtttcag gggttctggt ttccccttac cttcaaatgg
     3901 tgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct tatctgtttc
     3961 ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca ct
//
LOCUS       DADLXX010000221         3977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLXX010000221 DADLXX010000000
VERSION     DADLXX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.226"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..92)
                     /locus_tag="JNX27_004000"
     CDS             complement(<1..92)
                     /locus_tag="JNX27_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7884985.1"
                     /translation="MRAPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNX27_004001"
     CDS             complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNX27_004001"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7884986.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2205..3854
                     /gene="pgi"
                     /locus_tag="JNX27_004002"
     CDS             2205..3854
                     /gene="pgi"
                     /locus_tag="JNX27_004002"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7884987.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1008 a         1050 c         1004 g          915 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg tggtgccctc agtgagccag agagttcagt gtacgggaat
      121 tcgtttgcga gcagaactga taatattcat tttgccttct cttaactatt tatctctttc
      181 gataattaat tagagatgca gataaaaaaa ttaaaggcaa ttattctccg ataaagacgt
      241 tggttaacag agtacaggct cgtttattga tgagcatagt gacaagaaaa tcaatacggc
      301 ccgaaatata gcttccaggc catacagtat ttactcaaac aaattactat gcagtttttg
      361 caccacctgc tcggcatctt cgccgggcac caggaagcac aggttatggc tggatgcgcc
      421 gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg aatacctctt tgccaacgcc
      481 gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc gccagacctt cttccacctc
      541 cacccggcac agtgcggaaa gctccatcag cagagattgc gtcagcaacg tatcgccagt
      601 ggaggttgaa ccggtggtat caagggttaa tgccacgctc acttctgacg tggtgattaa
      661 gtctaccgaa atattatgcc gcgcgaggat gccgaaaact tccgcgagga agccgcgaga
      721 atgcagcata ttcaggctgt gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc
      781 gcggaacagc ggcggatttt cagttttatt gcacaccagc gtaccacctg cgcgtgggtc
      841 tttgctggag ccgacaaaga ccgggatatc gctgcgtact gcgggtagca acgttgccgg
      901 atgcagtact tttgcaccaa aagttgccat ctctgccgct tctgcaaacg cgatttcatc
      961 aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt
     1021 ccagatatca acacgagatg cgtgtaaagc ctccgccagc aaggctgccg tataatcgct
     1081 gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg ctaccgataa atccctgggt
     1141 gatcactaag ccttcattga ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc
     1201 ggctacatct ggctctgcac gaccaaatcg gtcgttggta cgcatcactt tacgtacatc
     1261 aaaccactgt gcctgaacat cgcgttcgcg caggatttcg acaaacagca gggtcgacat
     1321 cagctcgccg tggctgacca gctcatctgt cagcgccgga gacgttgcca gcgccgccgc
     1381 ttctgccaga acagtaatgt tctccagcag acgttcaatc tcttcacgga taacgttcgg
     1441 gtaacgcaga cgttccagaa tggcaaactg gatgttgcga atagcgtcga gtttttcgaa
     1501 tcgctcgcca ggttccagcc cttccgctaa agcgaccagc agattagtga taccagcaga
     1561 agccgagagg actactaaac gcacgttggc atcagaaagc acaatatcag cgctgcggtt
     1621 catggcgtca aaatcagcta cgctggtacc gccaaatttg gagacaacaa tttcagacat
     1681 aactacctcg tgtcagggga tccattttca gccttggcac aagggaagag cggaagacgg
     1741 gtgggcgcag agcgatactt cgctactatt ttcacccaga agtgctccac cacttgcgaa
     1801 acgcccgact gcgaacgctt ctggtgacaa cccaggggat tcagcccctg tagccgatga
     1861 tgaacgtggc cagccgttca atcacctcgg cgatgcaccc cctcaggtgt tatcacagga
     1921 ctggctcctc caacaccgtt acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg
     1981 tagtacattt ataaataaag ggtgagcggg gcggttgtca acgacggggt catgcggatt
     2041 tttcatccac tcctggcggt cagtagttca gctaataaat gcttcactgc gctaagggtt
     2101 tacactcaac attacactaa cggcactaaa accatcacat ttttctgtga ctggcgctac
     2161 aatcttccaa agtcacaatt ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc
     2221 caacgcagac cgctgcctgg caggcactac agaaacactt cgatgaaatg aaagacgtta
     2281 cgatcgccga tctttttgct aaagacggcg atcgtttttc taagttctcc gcaaccttcg
     2341 acgatcagat gctggtggat tactccaaaa accgcatcac tgaagagacg ctggcgaaat
     2401 tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg
     2461 agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca
     2521 ataccccgat tctggttgat ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga
     2581 agatgaaaac cttctcggaa gcgattatct ccggtgagtg gaagggttat accggcaaag
     2641 caatcactga cgtagtgaac atcgggatcg gcggttctga cctcggcccg tacatggtga
     2701 ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg
     2761 ggactcacat cgcggaagtg ctgaaaaaag taaacccgga aaccacgctg ttcttggtag
     2821 catctaaaac cttcaccact caggaaacca tgaccaacgc ccatagcgcg cgtgactggt
     2881 tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca
     2941 atgccaaagc cgttggcgag tttggtattg atactgccaa catgttcgag ttctgggact
     3001 gggtcggcgg ccgttactct ttgtggtcag cgattggcct gtcgattgtt ctctccatcg
     3061 gctttgataa cttcgttgaa ctgctttctg gcgcacacgc aatggacaag catttctcca
     3121 ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct gattggcatc tggtacaaca
     3181 atttctttgg tgcggaaact gaagcgattc tgccgtatga ccagtatatg caccgtttcg
     3241 cggcgtactt ccagcagggc aatatggagt ccaacggtaa gtatgttgac cgtaacggta
     3301 acgttgtgga ttaccagact ggcccgatta tctggggtga accaggcact aacggtcagc
     3361 acgcgttcta ccagctgatc caccagggaa ccaaaatggt accgtgcgat ttcatcgccc
     3421 cggctatcac ccataacccg ctctctgatc accaccagaa actgctgtct aacttcttcg
     3481 cccagaccga agcgctggcg tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg
     3541 atcagggtaa agatccggca acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta
     3601 accgcccgac caactccatc ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga
     3661 ttgcgctgta tgagcacaaa atctttactc agggcgtgat cctgaacatc ttcaccttcg
     3721 accagtgggg cgtggagctg ggtaaacaac tggcaaaccg tattctgcca gagctgaaag
     3781 atgataaaga aatcagcagc cacgatagct cgaccaatgg tctgattaac cgctataaag
     3841 cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat gtaggccgga taaggcgttt
     3901 acgccgcatc cggcaaccgg agcctgatgc caatatcgac attaatgcat agtatgagga
     3961 gttaaataac cttaaaa
//
LOCUS       DADLXX010000222         3963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLXX010000222 DADLXX010000000
VERSION     DADLXX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.227"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..746)
                     /gene="tnpB"
                     /locus_tag="JNX27_004003"
     CDS             complement(<1..746)
                     /gene="tnpB"
                     /locus_tag="JNX27_004003"
                     /inference="COORDINATES: protein motif:HMM:TIGR01766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY7884988.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTTTVSKNHVMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYE
                     MRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADV
                     NGARNILAAGHAVLACGEMVQSGRSLKQEPT"
     gene            complement(1011..1184)
                     /locus_tag="JNX27_004004"
     CDS             complement(1011..1184)
                     /locus_tag="JNX27_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7884989.1"
                     /translation="MKRLQVFKFRLRPGGQQAREMRRFAGACRFVFSRTLARQNENHK
                     AGNKDIPYAKMAS"
     gene            1252..1867
                     /gene="tnpA"
                     /locus_tag="JNX27_004005"
                     /pseudo
     CDS             1252..1867
                     /gene="tnpA"
                     /locus_tag="JNX27_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            2195..3277
                     /locus_tag="JNX27_004006"
     CDS             2195..3277
                     /locus_tag="JNX27_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY7884990.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3766..>3963)
                     /locus_tag="JNX27_004007"
     CDS             complement(3766..>3963)
                     /locus_tag="JNX27_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7884991.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         1087 a          915 c          829 g         1132 t
ORIGIN      
        1 gtgggttcct gcttcaacga gcggcctgac tgcaccatct ctccacaggc aagaacggcg
       61 tgccccgccg ctaaaatgtt acgagcgcca tttacatcgg cgttcgctgt atatccacat
      121 acctggcatc tgaatttact ttgtgacagg cgattttctt tcgctgtatg accacagcac
      181 gcgcaacgct ggcttgtgta cgctggcgga acagcaagca cctgaccgcc acgccagagc
      241 tgcttgtact caagctggcg gcgcatttca taccagccct gatccagtat cgaacggttt
      301 aaacctgatt ttgcccggac attgcgcccc ggctggctga ccgtacccgc tgctgacttt
      361 gacatgttgc tgaccttcaa atcctcaatg actatcatta cgtggttttt gctgacggtc
      421 gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg cagtcgctgt
      481 attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct taactgtcgc
      541 tgaagtctcg ccagcttctt ctggtttttc tgaaaactgt ttacaggctc aaagactgtg
      601 ccatctgaca gcgaggcaag tttagccacg ccagcatcca gcccgaccat tgatgctgaa
      661 gggtgaaccg gagttgatac ttcactttct gcctgaatac tgatgtacca cttaccgcag
      721 gactggctga cagtgacatt tttcacaaca cccgtgacct gacggctgtt ccggtagcgc
      781 atccagccaa gttttggcag aaaaatacgg ctgttttcct gatcgagctt aacgccctgc
      841 ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc aggggaacgc cgaccgcttc
      901 tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg actgttgcaa tggctgtgac
      961 ggagcatctt taatccattg cgtttcagtg gcatttttcc actcaaccaa tcaggaagcc
     1021 attttcgcgt aagggatgtc tttgttaccg gccttatgat tctcattctg acgtgccaga
     1081 gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcacg cgcctgttga
     1141 ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt tcacagggca aattatactt
     1201 tggtctgtga aaaaaactga tatccgacgc ggaaggcact gtgttttcct gatgcatgct
     1261 caccgggtct ttgttaccaa gtaccgacgc cagatttttg accatgacgc gacagaaaaa
     1321 ctacgtactt acttttcaaa tgtatgtgct gattttgaag ctgaactggt tgaaatggat
     1381 ggcgaaccag agcacgtcca tctgttaatt aactatcccc ccaaactggc gatatccagt
     1441 ctggtaaaca aacagcctca aaggcgtatc gggtaggtta ctgcgacggg atcggccaga
     1501 tattgctgcc aggtattacc gcaaagatgt tttgtggcgt cccggctatt ttgccagtag
     1561 ctgcggaggt gcgccaatat ccgtcatccg ccaatacatt gaacaacagc aaacacctgg
     1621 tcaggtgaaa aaacgagcct tatatccccg ccctgaagga cgggctttta cggtgcactg
     1681 gataagcctg gagattatct acacctgcat ggacatcatt gtactatgcc ccgtacatgc
     1741 cccatcacat cctcaacatc aaagtattag agccttgcaa accattatgc gtctcagttt
     1801 gtctcatcta tcttacgcaa aaaaaataga actaatatca agcggcgcta tattgcgtat
     1861 taaataaaac cacctcaatt acattaataa atctaaataa atatcatgca aataatttcc
     1921 atggttattt ttaggctatt taaattaaaa aaataaatta tgagtcaatc ataaataaca
     1981 acctttaata taatttgact accaaattta tattcgttca caaaatagtc atgcattgtg
     2041 tgcaatagaa acagttcaga taaaaatagg gatctacttc acaatttaaa tgagaaacta
     2101 aaacttacat cttgaaataa tctcattgat tggatgaata tttgtagtgc agtgacatca
     2161 tttttaaata atagttcaaa taaaagggct cacgatgaaa aaattaacag tggcaatttc
     2221 tgctgtagct gcatcagtac tgatggcgat gtctgctcag gcagcagaga tttacaataa
     2281 agacagtaac aagctggatc tgtacggaaa agttaatgct aagcactact tctcttctaa
     2341 cgatgcagat gatggcgaca caacttatgt tcgtctgggc ttcaaaggcg aaactcaaat
     2401 caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca accgtgctga
     2461 atctcaaggt tcttccaaag ataaaacccg tcttgccttc gctggcctga aattcggtga
     2521 ctacggctcc atcgattatg gccgtaacta cggtgtagca tacgatatcg gtgcatggac
     2581 cgacgttctg ccagaattcg gtggcgatac ctggacccaa acagatgtgt tcatgactgg
     2641 tcgcaccaca ggtgttgcaa cctatcgtaa caacgacttc tttggtctgg ttgatggtct
     2701 gaactttgct gctcagtatc agggtaaaaa tgaccgcact gacgtaactg aagctaacgg
     2761 tgatggtttc ggtttctcca ctacttatga gtatgaagga ttcggtgtag gtgcaaccta
     2821 tgctaaatct gaccgcacta atgatcaggt tatctacggt aacaacagcc tgaatgcatc
     2881 tggtcaaaat gctgaagtat gggcagctgg tctgaaatat gatgcgaaca acatctatct
     2941 ggctaccacc tattctgaaa cccagaacat gactgttttt ggtaataacc atattgccaa
     3001 caaagcacaa aacttcgaag tagttgcaca atatcagttc gacttcggtc tgcgtccgtc
     3061 cgttgcttac ctgcaatcta aaggaaaaga cttgggtgcg tggggtgatc aggacctgat
     3121 tgaatatatt gatgtaggtg caacctatta cttcaacaaa aatatgtcca cttttgttga
     3181 ttacaaaatc aacctgattg ataagagcga tttcacgaaa gcatctggcg ttgctaccga
     3241 tgatatcgtt gctgtaggat tggtttacca gttctaatct gattactcaa agatatgttg
     3301 tgggaggctt tgcctcccca acatataagt ggcgccctca agccactttc cttagaagca
     3361 ctatcttgct tctgactata taaattaccc tttattttct gggacgtttc aacgccccat
     3421 ttttaataat actcagtgaa caattaacgt atcaattaga tttaataaca acgatatccg
     3481 tacctaacct gatatctaat gtcataagca tccctttaat tatcccctca acctcttgta
     3541 aatttcccac tcttgagctc cacatagtcc aaacgccgga tgcaaaaaaa atcacacgtg
     3601 catctctgaa attgtgatgg aggaagtgag tatcaattat atgtgcttat aaacgttccc
     3661 gattggcaca gaagtcaccg gattgttcag gctccaatag gttgagtatg tcgtatttat
     3721 tttccaaaat aaaaatcgat gctgttgctc cttaataaaa tacttttact ctctggacgc
     3781 ttttcttagc tctcttggtg ttatcttaaa acgattatgg aacctttcag taaaacgaga
     3841 aggacacttg taaccatttt ccctggcaat ctcgctgatg ggttttactg tcgtttgtat
     3901 agcagacaac gcattattta acctcacgtc gtccaatata ctttgaaaac ttattccctc
     3961 gct
//
LOCUS       DADLXX010000223         3858 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLXX010000223 DADLXX010000000
VERSION     DADLXX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.228"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1447)
                     /locus_tag="JNX27_004008"
     CDS             complement(<1..1447)
                     /locus_tag="JNX27_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7884992.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDRTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQ"
     gene            1943..2862
                     /locus_tag="JNX27_004009"
                     /pseudo
     CDS             1943..2862
                     /locus_tag="JNX27_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(2949..3464)
                     /locus_tag="JNX27_004010"
     CDS             complement(2949..3464)
                     /locus_tag="JNX27_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7884993.1"
                     /translation="MTKLMQFVQRCYYMTNKKMYFILILVFTLLQVCFFALWKARDGS
                     TTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFF
                     TYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSI
                     PGLIINTCAPI"
     gene            complement(3418..>3858)
                     /locus_tag="JNX27_004011"
     CDS             complement(3418..>3858)
                     /locus_tag="JNX27_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7884994.1"
                     /translation="ITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIE
                     MFAVAFKDTYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVP
                     GANFIYDVNSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT         1151 a          732 c          681 g         1294 t
ORIGIN      
        1 tctgtttagt ttttttgagc gtctgtccac atttgaagat atcttcaggt tttggtctga
       61 aagcagtagt gttattaaaa taattttcat atacaggtgt gggtgctata gcattgcttc
      121 taattagatg gagaagatga tcatccatgt gttgtttatg ttttttatta aatatatttg
      181 ctaattcctc aggatcaatt gggtctttca tctgattaaa tggaacctgc aaagcaccga
      241 gacaataaca ttgattttct tcatttctta ttattccgtg atgactggtt aaaactttag
      301 tttctgagtt ataattacaa acatcgagcg ttttaattaa gttatacgta tcaccggcag
      361 agagtttata attttctagt cgtgcagtat agttaccatt aaaattgatt tcatgatttc
      421 cagccagaac gatgacattt ttattaatac ggctatcttt attaccctcc atatttcgca
      481 tagaatttag caaagtaagg atatatttat caccaaaaat agtactaaat ctatcgcctg
      541 tatgatcccc aagggggata caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat
      601 gcaactcatt caatatcatg tctctcgtat ttaataaatt gtagagcaat ttattatttt
      661 tatgtatgta tgcagatgaa gccatgtctt cgtgttttaa taattcacat aataaactcc
      721 aacccttttc cctgatgcct atatgccctg aaacgagcaa tgaataaagt acagcgccta
      781 ctcttccatc tgtatcgcca aaataagccg tgttggcatt ttgtgggtta atcacaccac
      841 attgtttata aaagtctatt ttagaaaaca cagatttaac atagctatca aacttatcct
      901 taggaaacgt ctctttagag acatcccaac gccagtcagc tttaaaatta ccgctaagaa
      961 gagtaaagaa attttcaggt atgatagaaa gcccattaac tttataaatt atgtctgatt
     1021 tatctccgga gaaattaata aaataataac catccttaca ctcaatctta actgtaatgg
     1081 gagacgataa ttttatcaca tggttattgt ctggtataat tgtaaagata aacgtgttag
     1141 tagtattatc ggcaaaacta ttatattctt gaatatctat agatggctca ctattgtaaa
     1201 accatatttt ctccatctga agactatgtt catttaccct ccagtgacca tgttgcagga
     1261 aatagatgat atgcatgcca aaatcgttat ctatttgcga atcattatcc agtttatcaa
     1321 tttcaactat ctttgaacga tgtgttctat caaccttgct atggtgttga tgattattaa
     1381 ctttatctga atgcttatct ttatggtttg aagggatgta cttaaatata ttgtcaattg
     1441 ctttcataaa taacccgcga tatttttgac ctgttagtaa aaaataaatg atacatgtct
     1501 gaaagacata tttacgacga tataaaacaa taatacttat atgttcgctg attttaagca
     1561 ccgagactat ccggtctttt actaaataat ggaaaacaat ccatccctgg aagacaattg
     1621 taaaaaaaac aacagaataa gaattaatct taggataaat atttatttat catggccttt
     1681 tgaccgctaa aaaatggcac tcggcacagt aaaccccgct atagttatgc tatacatttg
     1741 tctttaatga tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca
     1801 atattagcag ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa
     1861 ttaatccctt tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt
     1921 tcagggcaac gtcacgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt
     1981 cactaccgca tttattatca tttcgcagct cacgcgcggc gttaatacgt tttacaatac
     2041 attgttcccc attattggct atgcggggtc aattatcacc atgatatggg gttgggcact
     2101 gttagcaggt aatgatgtga tggcagacga gtttgttgcc ggccatgtta tttttggcgt
     2161 tggtatgatt gccgcatgtg tatcgacggt ggcagcgtcg tccggtcact ttctgctcat
     2221 tcccaaaaat gcagcgggga gcaagagcga cggaacaccg gtacaggctt attcttcatt
     2281 aatcggtaat tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc
     2341 tattacgctg ttacatagtg ccgacataac tccgcattat gtcgccttgg gttaaccgca
     2401 atctgtgcct gtctaattgg cctagtggcc acaatcgtcc atcaaacacg taatacgttc
     2461 tcatccaaag agcactggct gtggtgttat tgggttattt ttctcggctc agtcacggtg
     2521 ctgcagggga tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt
     2581 attctcattt gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca
     2641 ctggtctgga gacgtacctg ttcgttagcc aacagaatac cgatgatacc ggtatttacc
     2701 tgtctgtttt gcttgttttt gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac
     2761 tactttattc catctcgggt tctggttgga ttaagcgcag tatgttttac attgttctct
     2821 attgtttcaa tattagaggc cggttctgcg aaaaaaaatt aaaatttaca taatcccagc
     2881 gttattcata tcttaacgct ggttttttac gaacttagtg aaataatgta gaatataaaa
     2941 ttataatttt atattggagc acatgtgtta atgatcaacc ccgggatact aaaaatatag
     3001 acattattaa acttgcttag cctcgcattg cctttacctg ttaaagatgc atcgagaaaa
     3061 ttgagtctga agttctcatc ctttatctga ttttccagtc ccgaaacttt ggccttagca
     3121 ttacctgaga tccgtccgtt actatctatt ttgtaggtaa aaaaatacga atgggaaaat
     3181 ttcctcggtg tatcttcatc agttaagcca acaatcgtaa aactaccgct accatctttt
     3241 tttaaaatga ctgaaaggtt agcagagtag tataaagaat gatctgtttt agcatttctt
     3301 gttaacgtcg acgtacattc aagagacgtt gtgctaccat cacgagcctt ccagagagca
     3361 aaaaaacaca cctgtagcaa tgtgaaaaca agtattaata taaaatacat tttcttatta
     3421 gtcatataat agcacctctg aacaaattgc ataagtttcg tcattagagg attttataca
     3481 tcttgataaa taccgctgcc cttccccaag caaataatgc atactattta catcatatat
     3541 aaaatttgca cctggcacac agggtaaatg ctttttcgaa atttcagcgg cctctatttg
     3601 agatttagcg aggccaatct cgttaagatt ctctgaaaga tataaatcac aggtgtcaat
     3661 cttacctgat ttaaccaatg gataatccgt tatactgtag gtatctttaa aagccacggc
     3721 aaacatttca ataataacaa ttactaccac aagcgaaatc aaaatgcgaa aaagaggaat
     3781 actccttttg cgctctactt tttcaacaac atcttcttgt aaatgagaat catcataaga
     3841 ttgtttactt attgttat
//
LOCUS       DADLXX010000224         3671 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLXX010000224 DADLXX010000000
VERSION     DADLXX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.229"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(623..2332)
                     /locus_tag="JNX27_004012"
     CDS             complement(623..2332)
                     /locus_tag="JNX27_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7884995.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="JNX27_004013"
     CDS             complement(2764..3483)
                     /locus_tag="JNX27_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7884996.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1087 a          742 c          660 g         1182 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcactct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt ggctcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgcga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgcagtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt g
//
LOCUS       DADLXX010000225         3582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLXX010000225 DADLXX010000000
VERSION     DADLXX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.230"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            115..645
                     /locus_tag="JNX27_004014"
     CDS             115..645
                     /locus_tag="JNX27_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7884997.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYNEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            652..885
                     /gene="mbeD"
                     /locus_tag="JNX27_004015"
     CDS             652..885
                     /gene="mbeD"
                     /locus_tag="JNX27_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7884998.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1464..2582)
                     /locus_tag="JNX27_004016"
     CDS             complement(1464..2582)
                     /locus_tag="JNX27_004016"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7884999.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2700..2789)
                     /locus_tag="JNX27_004017"
     CDS             complement(2700..2789)
                     /locus_tag="JNX27_004017"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885000.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3225..3386)
                     /locus_tag="JNX27_004018"
     CDS             complement(3225..3386)
                     /locus_tag="JNX27_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7885001.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1092 a          675 c          845 g          970 t
ORIGIN      
        1 atgtgattca ggcgctcaca gaggcaggga ttgacgtggt cagggtgacg cgaagcagta
       61 tcagcattgc agacccgaac ggcgggaaga atgtcagact gaaaggagcg ttttatgagc
      121 aatcttttac agacgggtgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
      181 gagaaaatgc tgaacgacga gttcaggaag ctcgaagaat ctgtaaacag ggaactgacc
      241 tcaaacgaat ctttaatcag gaacgctatc aacgaccata catcagccct gaaagaactt
      301 ctggagaggt atcagaaaac gaccgtcgat acgatggacg cacactggaa aaccgtactg
      361 aaaatgagcg tgaaacgctg gctgtggctg attatcgtct cagtactgat gtttgccacg
      421 accgggagcc tgctgtggtg ccagggcatg aagataaacg cgaacatgaa tattctccgc
      481 gagcagaaag agagtctgga gaagctgaac gcgaagacat ggggcgtgcg ttataacgag
      541 gacagcaacg gacgttttct ggtgttgccc gaggggatga aagccgaaac gaactggacg
      601 atggaaaacg ggaaacagaa tgccgtaagg ctggtacagg agtaacagag catgacggag
      661 ctggaaagac atttgctgaa cgcattagag caactgcaac aggactatat gcagcggctg
      721 aacgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacgggac
      781 aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag
      841 cagacagaac gcttgagcca gttatacagc gagaacagga gataagggac gaacggctga
      901 tacaggaatg cgaacatgcg ttatccctgg agcgtgagcg acagctggaa atacaggaac
      961 ggacactgga tggcccttcg ctgggatggt aatcagagac aggctaaaat gttgtcagcg
     1021 cgtttccggt gcattttttg ggataatggt tggtttgggg gcaccgggaa cgcgcttcag
     1081 tcagaccttg ctgaaaacat aggaacgcgt taaaacgcat acgggcgcga ttctggtgtg
     1141 tcttttgttg ctgtgttttg cctctattgc cagatcgcgc tcattcccct gaacttctgt
     1201 tttcccgctc aggtccccgt cttcttcgct tccgaaagca tgaaaaaaat ccgtttcagc
     1261 tgtctctggc tggcaattcc tgcgtgattc acatggctgc atagctatgc atgtggttaa
     1321 aatttaccgg gtgcgatcgc ggcagttttt cgggtggttt gttgccattt ttacctgtct
     1381 gctgccgtga tcgcgccgaa cgcgtttcag cggtgcgtac aatgagatgt tatggtaaat
     1441 tcaagtctta gattttatga tgtttaatta gctttttttg cgtttataat gttcatcttt
     1501 tttattaaaa ttgattcaat ctttgatgtg ggtattaata ttttctttga caaatagaaa
     1561 gttaaaaaaa acaccattaa tagtataaat aaatacccaa aaacagaagc gttattaatt
     1621 tcaattattg atgttaatct gtatattact acatgctgaa tcaacatgga tatgtagatg
     1681 catttagcat atgagttgaa aaaatagagg ttaaatttct tattaattat taatgctgct
     1741 ttgtaaatga taatgcttat gcacattaaa ggtattattg ataactggtg attgactatg
     1801 tttgttttga actcattagg ccctattaat aatactgtca atatacatat aacaaccgat
     1861 agaataactg tggtgttttt ttgtaattta tttttgtatt taaatgcaag catcccaacc
     1921 ataaactcag gaagtcggca agcaaaaaac caaaatggga agtttaaaaa tgatgatgag
     1981 atatttgata tcgggaatgt tgtaattgtt atcacaaata aaaaaaatat gttacgttta
     2041 aatgtaaagg tattcataac gtgaaaaact atcggtgata tcacgtacag cataataacg
     2101 cacccaataa accactcgcc tgtaaggaaa agaaaatcat aagagaaatg ggcgtttaat
     2161 ataccatcaa gggcaaaaaa tactggaatt attctagtgt actggcctcc tgttgtcatt
     2221 attgatacga gagggactgt attaattatc tctttattta aataagacaa taaaattaag
     2281 agtaaagaga taaaaaagta agtgataata taaggagtaa ggatccttac tcctttgtca
     2341 aggaaataat caatggtgtt ctttctatct agtgaaataa atgccaaata tccagatata
     2401 aaaaaaaaca agctcacacc tacctggcca agaacccctc cataaaatga tggtatgaaa
     2461 gatagcctgg tttggctaaa tatgtagcca taatgagata taataacaaa aatgcaggct
     2521 gttactcgca atgtatcaat tgacggatct ctattagaca taacctttat ttcgctgttc
     2581 aaattatcgc ctttagttga tgatgtaaaa ttaacgctat aaataagcta cacattctag
     2641 agcagcgaaa ggatgattca agtattttta ataataaaac gcattgataa tgtttatgtt
     2701 cagatggtgc tgatagcttt gtcatgctgt tccactgatg ttgataacga cagagacttt
     2761 tgtccttttc ttgttagatg ctgcctcaga ttcggattat ggcgttaaag gggtttgacg
     2821 ctcaaccgaa cggtttcaca cgttaagagc tttttgtctg ataaatcaaa atataatgga
     2881 cattgaaaaa cgccctcaaa acggtgtctt tttaatcgaa gattggcaca aaacggacac
     2941 caccgtccat tagtcgattt tttagacggc ttttctcgcc taacgtgagt tttcgttcgg
     3001 ttgagcatca gacccctttt atattgtaga tttctatttt tagacagttg gaacaggaca
     3061 attaacaaca gatctacttg agatcatttg cttgtcgtcg tagtctcttt cccagaaacg
     3121 aaaaaaccgc cctgcaaggc ggtttctcga aggttctcag agcgaccaac tctttgaacc
     3181 aaggtaactg gcttatgagg agcgcagtaa ccaaatctgt cctttcagtt tagccttaac
     3241 aggcgcataa cttcaagact aactcctcta aatccgttac caatggctac cgccactggc
     3301 gatttgtcgt gtctttccgg gttggactca agttgatagt taccggaacg ggcgtggtag
     3361 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     3421 ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa
     3481 ccgaaaggca ggaacaggag agcgcacgag ggagccacca gggggaaacg cctggtatct
     3541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cg
//
LOCUS       DADLXX010000226         3542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLXX010000226 DADLXX010000000
VERSION     DADLXX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.231"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..222
                     /locus_tag="JNX27_004019"
     CDS             <1..222
                     /locus_tag="JNX27_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885002.1"
                     /translation="IANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGV
                     GPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(863..1135)
                     /locus_tag="JNX27_004020"
     CDS             complement(863..1135)
                     /locus_tag="JNX27_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885003.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            1224..1403
                     /locus_tag="JNX27_004021"
     CDS             1224..1403
                     /locus_tag="JNX27_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885004.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1455..1649
                     /locus_tag="JNX27_004022"
     CDS             1455..1649
                     /locus_tag="JNX27_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885005.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(2448..2784)
                     /locus_tag="JNX27_004023"
                     /pseudo
     CDS             complement(2448..2784)
                     /locus_tag="JNX27_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1132 a          760 c          693 g          957 t
ORIGIN      
        1 atagccaatt ttatcgcatt acaaataaat ggttcaaaaa caggatttgc cgcatctatt
       61 acaggtttta ctcaaacaat aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc
      121 gacaacggtg ttggtcccgg gactacttta ggtcttggtt cactaataac ttcattactg
      181 tcaatagccg taatcgccat tgcaggaaat aataaaaagt aagtgtactc tgactgcgta
      241 tgtatctact ttgtgtaatg caggtgtcta ttgaaaataa ttcttttcgt gggcatctgt
      301 tgtataaaat aaaaaattat cgtaagtact ctatctgcga tgtcttgtga aaattatcct
      361 gtctggcaac aatcgcgtcc atctgtatta atggacgcga acgatgaact ctcaaacaac
      421 actagatatc accggctttc gcacttattt gccagaatca ttacgtctca gattcgacga
      481 gcaattatct gtccgggcta tcgcacagca gttaagactc agttattccc ccattcacaa
      541 tctctttcaa cgaattaacg cctcaggtat tgtctgacca tgacccgagt ccctttcatt
      601 cgcccaaccg ataccatact ttatgccaac agaaagaatg aatgaaccgc gcctaaaatc
      661 agcaaggaca catggcacaa agtgcgacat gtctcacatt taaccccagt gggggcaaca
      721 agtataaaac cgttttttga ttaagtggtc gaatatgaaa aggggaaggt ttatgtgaaa
      781 aatacctgtg ttttctttga aaacacacaa cagtatcagg ttatttatgt ttatacagtg
      841 cgttccaaac tgacctgaat aattacgcaa gttgcaacaa taaaacattt ttgccgtagt
      901 gtgaggtact ccggtaaatt gctttccagg tgcagacaat gactaacgaa gaactacagg
      961 caatcgcatt atccatgctt gagcgccagc gcgaaaaagt acaccagcaa cgagagtctt
     1021 tgcttaaccc atctccattc accaggccaa aacgattgga gcttgaagca ttcttggaca
     1081 aatacccacg gcggcttaaa tcaggcaaaa ccagaccacc aggctgatag ctcacagttt
     1141 gttctccgat tcaataaaac ggataagaac catcagcgcg gtagcgtaag gcactacagc
     1201 gcattgcttt acgagtaata acaatggatt tatcaccaga agacgcaaag tttttacgag
     1261 aactgcaaca ccgtatgctc agagaagcca ttatcgaaca gcaaacgggc atgagcgcca
     1321 tagacccatt gcgatacaaa cgggcctcat ctcctttagt tgacgacaat tccaatgcgg
     1381 ataaaacacg tccaccacac tgattacatt tttcattagc acagtggtgt gatgcagcat
     1441 acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct ggagcaactg ttacaggcac
     1501 gccaacggga gtttgccact aaatggggac gtgccgcgct gccacatgaa cgcctaacct
     1561 attttccact tgaagacctc attaaaaata atggcaaaaa aatcaggtct gataagaaac
     1621 acttcttaga atgcaatgaa gacaaataac aacaatctgc atattgccac caaccacaga
     1681 tgtggcatcg ttatcagcgc acttgatttt ctttatatcc actgacgagc ttgccggggc
     1741 acctttgttt gccggtcatt ctggtatcat gcagatattt acggcgtaca agcccactag
     1801 catgacattg taataaggcc tgagcctaac gctttaatgt gtgtcataca ctcagtggcg
     1861 taatcgttga caatatggtg catgtaatcg taagcgtcca gcgaaatcca tctggtcata
     1921 atctgaagca tccgacaaag tggtgtccac caaataagtg atctgcgccc aatatcctga
     1981 gccggaactt tgacaaaggc caacgaaaaa tgggagcgga tgttacagag ttctcggtac
     2041 aggggaaaag tggtacctgt cgccagttct ccgatctgtt taaccgggaa attatctcct
     2101 acagcttgtc agaaaggtcg gtgatggagc atcgttaata cgatgctata cgatgcattc
     2161 acagtgcccg gccagaggat gccccgtcgc tgcatatgga tcagggttgg caatatcgaa
     2221 tcgcaggcta taggcaaagt taaagcccca tggagtagca caaaatatgc cgtacaaagg
     2281 aaacggtctg aataacgcag tgatgaagaa cttcttcagc acactgctaa aacgcatagt
     2341 gatcgagcgc tgattctggc gaacaactga actaatacat cagaatctgc attatgttaa
     2401 ataaatatga aaagatggtt taaatacccc gttacttttg acttactcta tacggtatcg
     2461 catcgtttaa tattcgcacc ggccagattt ttatttctat tagttgtcac aatactgaat
     2521 gcgtacgacc acagtattct ggctcctgtg tggttatgct ttaattctgc gttccgggca
     2581 gataagcagt tgcttgcagg aatccttctt gtgttaatgt cagttcccct ttttaccagt
     2641 gctgatttcc acattccgtc caacagagct tatagccttt ccctggatta tagcattgtc
     2701 cggctgaagt tctttttgaa taataataga agcactgctg gcagatccag tccgtttttc
     2761 ataacccact gtactgataa ccataatcta atcagtagaa attgagtcga aaataagcac
     2821 tactccatac aggataatta gaggtcagtt tgattattca caattcatca tcagcatttt
     2881 ctatttctga cgaaatcaac atgaaaataa ccatatatga taattattat aataacggct
     2941 ttaattggaa tacatatatt acaacgtatt atatataatt ggtattctgg gaactatatt
     3001 ctcaaaatac agtagaaacg aggtatgttt ctggtggaaa ggacagtggg attaaaaagt
     3061 aagaattgat aaaaaaaacg ccagcaacac atagtgccgg cggagggaat accccatgga
     3121 gaaaatgtga tgcctagagc atcatgaata tataattaaa aatagttagc gttgtcaata
     3181 ctttcacaaa aataattttc gtagtataga aagatatttt tatgcatgac ctacctgaat
     3241 ttgctccggg tagaggttat aaataaaaat tgaatcacga caaacacaat attcatagta
     3301 tggcgatgcc tacgccagca agaatagcgc caataatatt gggaatataa tagacaccag
     3361 acgcacaggc atctcactcc ttaacaaaca ataatcagga tatttacttt tacccagcta
     3421 actgtttaca cccaaagtgc acacaattaa ccattcaata acaaatggca atatccatag
     3481 ccatacgact ttaccttgta atgttcggta tttctttata attattctgg gaaatctatc
     3541 at
//
LOCUS       DADLXX010000227         3517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLXX010000227 DADLXX010000000
VERSION     DADLXX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.232"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNX27_004024"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNX27_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY7885006.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNX27_004025"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNX27_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY7885007.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3517
                     /gene="cusC"
                     /locus_tag="JNX27_004026"
     CDS             2335..>3517
                     /gene="cusC"
                     /locus_tag="JNX27_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY7885008.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEKAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          864 a          911 c          920 g          822 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaagcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc actaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaaa aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaattga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcctttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagc
//
LOCUS       DADLXX010000228         3485 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLXX010000228 DADLXX010000000
VERSION     DADLXX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.233"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..298
                     /locus_tag="JNX27_004027"
                     /pseudo
     CDS             1..298
                     /locus_tag="JNX27_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(860..1807)
                     /gene="icsP"
                     /locus_tag="JNX27_004028"
     CDS             complement(860..1807)
                     /gene="icsP"
                     /locus_tag="JNX27_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7885009.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3188..3340)
                     /locus_tag="JNX27_004029"
                     /pseudo
     CDS             complement(3188..3340)
                     /locus_tag="JNX27_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3396..>3485
                     /locus_tag="JNX27_004030"
     CDS             3396..>3485
                     /locus_tag="JNX27_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HAY7885010.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
BASE COUNT         1055 a          697 c          655 g         1078 t
ORIGIN      
        1 tcatgctacc gggctaatga aacacgccag ttcacccgga tgttgggact ttgttgaacc
       61 gaagaacacg gcggtgcgga gtccggagag taacagaata gcaaagagct tcgtgaaaac
      121 aataaagtgt gactacatca gtatcatgcc caaacctgac gggttaacgg cagcaaagaa
      181 ccttgcagag gcattcgagc attataacga atggcatccg catagtgcgc tggattatcg
      241 ctcgccacgg gaatatctgc ggcagcgggc caatgataac aggtgtctgg aaatataggg
      301 gcaaagccac ccacgaacga tccagcatat aatcaagaaa ttagatttca tttaacataa
      361 gatagattat gtgcaccaag aggcgtttcc ttaatttctg aatttacagg ctcttttgct
      421 ctctttgcta tgctgtggat ttggaaagat agagtgctgg ctgattattt aggtattttc
      481 atcagcatgt ccctgatagc actgttccat cagcgctttc aggcgataat agtagacctt
      541 gggtagcctc tgcccatgtg taacttgata ccccgagaac agccacgatg atctaagcgg
      601 ttcttatttc tccggtctca gggtcggtaa tgccaacagt aagtccacag taatcaacga
      661 agagtttttt gaaccgcccc ggaaatcctg gagactaaac gccctgagaa agaggtaaac
      721 agaatgacta aaaatacacg tttttccccc gaagtccgtc aacgggcagt tcgtatggtt
      781 ctggaaagtc agggcgaata tgactcacaa tgggcggcaa tttttagcca tgaaaacgta
      841 atcaactcgg tgaaccaatt caaaaaatat actttatacc tgcggaagtg agaaaaccaa
      901 tatattcaat acttgcacta tttttaatgg tgccagatat attggtactt tgctcataaa
      961 gagatgtatc acctttttta ttagaaatgt aatttctgga gccttctata taaaattttg
     1021 cactgggggt gacatagtat ccaatattcg cagcaacaga gcagtaattt tggtttttta
     1081 tttcatcttt aaaaattgtc tcagtctgat agtgtttatc tgtatcagat gaaagaaccc
     1141 aaccactata tttcagttct gctccaaact caaaattctc atggcggtaa ttagcagtta
     1201 atccaatata aggtattttg aagagctgtt tgtaacctat tgttctttca ccactaggat
     1261 gtgccccttt tttattcctg ttccccccgt tttcactata aatataactc cctcccattg
     1321 cattaaaact gtaacgactc tcttggtaac ctgctattag tccaagtcga taatccaaat
     1381 tatttaataa ccaaccttta agattcagct cgaattcgtt agcatcacgg agatgcgtat
     1441 tgggatgcca gctctggtcg gtccatacct gaggggtatt ggaattgttc cagtctttat
     1501 caaccatgct tgctttctgg ttacctaaag tagtccaacc ggaaactccg aaagacactt
     1561 ttggaattag cttccaatcg atgccacctc taacaatagt cgcattactg tatttccagt
     1621 ccagttgact aatttttctc cccccttcct tgggatgata aacgcgttct tttgttttgc
     1681 cactcagaga tcctagacta atatccgtac tgatgttgtc cggtatgaaa agtggatagt
     1741 tagtggtagc atgtgtagta aagatcgcag gtacacaaag tgcaagtaca aggaattttg
     1801 atttcatcga gaactctact tttttggttg aaatgtccat gatcttctta ctttataaga
     1861 taaacgtcaa tgccaagcat tattttaatt aaatatttgt ttatgttacc aagaacatat
     1921 cttttattat aatatttatt gaacttttgt aaaaataaaa aaatacctca ttgtgcgaat
     1981 aaagtaacgg gggccataga tttctctacc taccttttgg tgttcatacc tgaggctgat
     2041 agtttcagga ttccgcgaca attctactca ccttcttgtt ctctcgccaa tatgaaatca
     2101 atcagaaaag cgttccataa gaacaacgat aagacaataa attgtcatca cggctgcaat
     2161 agccaacgtg cctttcagta ccaacatgac aacctcctgt atttgacgta acgtgtgctg
     2221 ataaatatgt atttgtatct caactgactc aatacaacac ttaagtatag tttttcagat
     2281 tttgtttttc ccgtgaaata ttatggaaat ggcagaagca atacattgaa ttccgattcg
     2341 agtgaatacg gctagcgggc acctcacttt agcactgaag ccatcaactt ttatttgact
     2401 tatttcaatg gaaagctttc tcactatcag tacaggtaat tcccatcctt ttaaagtcga
     2461 gagtcagact gcgactaata cagttaaagt gaccaaactc agttatcctc tacacatttt
     2521 acctcatgtt tacttggaca attcattggt gcttttataa aagaataaat attctactaa
     2581 tttaatgcat cacctaaaat agtatttttt ataatagaac tgtatctata gtatattatt
     2641 aaagcttaat tatttattat gctttaatgt ggttgtccga ttaaggacta atccttgaaa
     2701 acgccgtata gggcgggagt tcactctatt gcgataacgt aaccgtacaa tattgtgcgt
     2761 ttcccaaatt gaccactact gaactattct gggatttaaa tgcaataaaa tggttggttg
     2821 aaggtcgtgg tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata
     2881 ataaagaggc ttggcagttt ggataagcta ttgtccttat tccactaata caaacttttt
     2941 gtaaagtata ttaaatatac ttcatttcat actgaaatcc ccggaagtat tcaatcaaac
     3001 aattacacca taaaaggaag agggagcccc aattaagtta tcaagcatgt ttatgatacc
     3061 tgcagggtat aaattttctg gctgcaagca gacacatata aaacgtcttt attgatccta
     3121 cccacataat atggacgcag tcgtaagtga ggctctgtga gcctacccac gtaatatgga
     3181 cacaggccta agcgaggttc tggttttcaa attgttccgg actgaggccg ccacaccaac
     3241 ggtgccgtcg ccagcgattg taatcgcact cgatataatt aaacactgtt gcccgcacta
     3301 tttcccgata gataaagtgt tctccgtgga gacattccac ttaggaacaa ttatattgtt
     3361 catggtaatg tttatatttg agtatagaga ttaaaatgat tagtcccatc aagaatatta
     3421 agaacgtatt tccaataaat actgcaaata ctgaatatat agtgagaaat atttatccta
     3481 gagtt
//
LOCUS       DADLXX010000229         3388 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLXX010000229 DADLXX010000000
VERSION     DADLXX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.234"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(185..880)
                     /locus_tag="JNX27_004031"
     CDS             complement(185..880)
                     /locus_tag="JNX27_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7885011.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(873..2300)
                     /locus_tag="JNX27_004032"
     CDS             complement(873..2300)
                     /locus_tag="JNX27_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7885012.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2311..3030)
                     /locus_tag="JNX27_004033"
     CDS             complement(2311..3030)
                     /locus_tag="JNX27_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7885013.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          847 a          945 c          708 g          888 t
ORIGIN      
        1 ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg taaatatcct ggcattacta
       61 atgcctggat atttacagaa gagcagcata accatgacgg aacggggtta agtcaggttg
      121 tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc
      181 tctctcagca atcctcaata atcagataca ccgctttcac cggaccatga accccgacga
      241 ctttgataag ctcaatatcc gccgttgagc tgggaccact gatgatgtta atacatgacg
      301 gcattcgttc accggcctgc gctttctgat gcaatttttc tgcgagttgc gctacacgcg
      361 gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac
      421 gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag
      481 catacacgac acccacttta gcctgctctg cctgcgagat attctcggca cctttcgccg
      541 gatcccaaac aacggcattg cattcctgct gcaaacgttc actaatcccc aattcctcca
      601 gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac agacgtattg
      661 cagcttctgc cgccttcgcc tcgctggtca gctcacagcg cgtcaacata acatcgctgg
      721 caaactgaat aaacgcgtca cagcgctgct gttggttaag ttgggtaagc cgctcgttag
      781 catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg
      841 cctgagcaac gttattcaaa aattcgctcc gattatccat tctttttctc ctgcgcctga
      901 tgtttcttaa accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc
      961 atccagtcgc taatcgcgcc aaatttgagt ggtgttttac cgccattgat aaaccagctt
     1021 gccgcatgag cgccggccat catcccgact ttccacaatc ctggatgact attggcataa
     1081 gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc
     1141 cgacgatgac gcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa
     1201 gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtagc
     1261 ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata
     1321 tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg cagcacatcc
     1381 cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct
     1441 tcaggaccat caacgtgccc cgcttcgcgc ggccctgtca gccaggtgtt gtatcccgtc
     1501 aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac ctcggcaaac
     1561 gtgggggcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac
     1621 attcgcgcat taccttcatt ggtcaccagg cataccgaac cggtctctgc caccgcgaaa
     1681 ttacagccgg taatacctat ttcagcactg aggaaatctt cacggatttt ttgccggatg
     1741 aataaggtca tcgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcact
     1801 cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga tggcggatct
     1861 tgatccagct ggagaatata ttcacccaga tcggtttcaa tcacctgaat gccagcatcc
     1921 tgcaacacat gattgacacc aatctcttcg gtcaccatcg atttagattt caccaccttc
     1981 cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc tttggttttt
     2041 gcaaaataga cgtgaccgcc gttttgcgtc actttttctg agagctggta cagataagcg
     2101 tcgagattac tcaaaacatg atcacgtatc tgggcggccc gatcgcgcca ctcctcccag
     2161 tgccccaatt catcgaccat tttttgccga tttgccccaa tacgctgctg cgcgtttgcc
     2221 accgctttgc gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta
     2281 ttactggttt tgatcgacat ctttatatcc tcagcggctc atcaacactt cagcaatatg
     2341 catcactttg actttctgcc cttcccgttg taatcgccca ctgatgttca gcaggcaact
     2401 cacgtcagca ccaattaaat actcagggcg gacttccatc aggtgcacaa ccttttcttt
     2461 caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca
     2521 ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag
     2581 tggctcgtcc ttcactccca gcttacgggc caggctacaa gatgggtgat acacagctct
     2641 cccttgcaaa ctggcaccta catcgactac ccctaattta ttaacaataa aagaggtgag
     2701 atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt catccgccag
     2761 atacgtcggg taacttttta cggcataggt gcaagagcca gccggtgaaa taatgggatc
     2821 gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata
     2881 accgctattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca
     2941 gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc
     3001 aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt
     3061 atgcccgatg tttccccccc accgggggat caatcatcaa taaatatggc gttcttttta
     3121 aattggctag caacatacca cccctcattt gtatgacaaa tgataatctt tcatccaaac
     3181 ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat ggggaaatcg
     3241 acgatcattt ttggcataag aaatccattt aaaaatggct acatccctta tacctattca
     3301 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac
     3361 aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DADLXX010000230         3276 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLXX010000230 DADLXX010000000
VERSION     DADLXX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.235"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            303..422
                     /locus_tag="JNX27_004034"
                     /pseudo
     CDS             303..422
                     /locus_tag="JNX27_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            672..1538
                     /gene="ospB"
                     /locus_tag="JNX27_004035"
     CDS             672..1538
                     /gene="ospB"
                     /locus_tag="JNX27_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7885014.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1867..2607
                     /locus_tag="JNX27_004036"
     CDS             1867..2607
                     /locus_tag="JNX27_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7885015.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2960..>3276)
                     /locus_tag="JNX27_004037"
     CDS             complement(2960..>3276)
                     /locus_tag="JNX27_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY7885016.1"
                     /translation="KKADIALYLLSKFNFIKQDIAEMEKMNNNIYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRF
                     ER"
BASE COUNT         1049 a          607 c          616 g         1004 t
ORIGIN      
        1 cactccactg gagacgacgg aagatcgttg ccgaagcggt gttccaccgc tcccagcatg
       61 tcgccaatgg gagagcatct acggtctgat tgaactgttt attcaaattg tccaccgtct
      121 gtcagtacgc tctgaacgtc ggctggttaa aacactgctc gctgattttc agagggttca
      181 cggtaaaaca gcacttttat tccggattgc ggaagcggct ctgaataatc cggatgggct
      241 ggttaaagag gttgtgtacc atctgcatat tgttgaacca gatcgcagcg gtaagagaag
      301 ttcatcatac ttggcgcagt tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg
      361 cagaacatta ccggaacaag atagcggtct acctgcactg gtatcagacc cggggcttcc
      421 ccggatgatc tccacggtac tttaggtact gtgtctatat tatgtaggta ggatcaatta
      481 actttgtagt tgaaattctt tcattgaaat aatgaataaa aaagacaaac cgcattaaac
      541 aatgccagaa tataattgca tttttgttcc gtttttttaa atcatagtat acaaagaagc
      601 ttgcatacta tatgaaaata taacaccctt tactatgatg tttataaaac aatatatgga
      661 gtcatgtagg tatgaattta gatggtgtta gaccatactg tagaatagtc aataaaaaga
      721 atgaaagcat atcagatatt gcatttgcac atataataaa aagggtaaaa aattcatcat
      781 gtactcaccc aaaagcagca ttggtttttt taggagagaa aggtttttgt gatagcaatg
      841 atgttctatc tattatggga caacaaatac caagagtatt taagaacaag atgttatatg
      901 attatgtttt taaaaatgaa aaaagtaaaa atgattttct aaaaatggct gaatcatggc
      961 taccacagag tgaaccaata gtaataaata atgatgatga cgcattgaat gctgctgctt
     1021 atttttctgt aaaaaaagcg aaaataaaaa cagtaaacga tactgatttt aaagagtata
     1081 ataaggttta tattcttggg cacggtagtc ctggttctca tcaattaggc cttggttcgg
     1141 aacttattga tgtacaaaca atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga
     1201 aagatatccg ttttacttca tgcggctccg ctgataaagt ggctcctaaa aattttaaca
     1261 atgcccctgc tgaaagtctt tcttgtatcc ttaactctct gccttttttt aaggaaaaag
     1321 aatctttgct agagcagata aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc
     1381 taaaaatatc cggctatcat ggatatggag ttcactatgg tcaagagctt tttccctact
     1441 cacattatcg ttcaacttca attcctgctg atccggagca tacagtaaaa agaagctctc
     1501 agaaaaagac ttttattatt aataaagaac tggattagta taaaattttt aacctatagt
     1561 taattgtttg catttaagat gaagttattt tatctttagt agaaaagtgc ttccctatga
     1621 gggaaaatca caaatcatca taatacagag acaaaacgat acgaacaaat agataaaaaa
     1681 catcattctt ttaccacatt atttttcgtg aatatgaaaa ataatgttat tactttaata
     1741 taagactatt ttttgttttt ccatcactct gttcaaattt ttccgcatga cttgtgtttt
     1801 ttgtaacaca gctcgttttt tacagctgac caaaatcatc aattaattat gctaaggaaa
     1861 taaattatga aaaccaaaaa ctttcttctt ttttgtattg ctacaaatat gatttttatc
     1921 ccctcagcaa atgctctgaa ggcagaaggt tttctcactc aacaaacttc accagacagt
     1981 ttgtcaatac ttccgccgcc tccggcagag gattcagtag tatttcaggc tgacaaagct
     2041 cattatgaat tcggccgctc gctccgggat gctaatcgtg tacgtctcgc tagcgaagat
     2101 gcatactacg agaattttgg tcttgcattt tcagatgctt atggcatgga tatttcaagg
     2161 gaaaataccc caatcttata tcagttgtta acacaagtac tacaggatag ccatgattac
     2221 gccgtgcgta acgccaaaga atattataaa agagttcgtc cattcgttat ttataaagac
     2281 gcaacctgta cacctgataa agatgagaaa atggctatca ctggctctta tccctctggt
     2341 catgcatcct ttggttgggc agtagcactg atacttgcgg agattaatcc tcaacgtaaa
     2401 gcggaaatac ttcgacgtgg atatgagttt ggagaaagtc gggtcatctg cggtgcgcat
     2461 tggcaaagcg atgtagaggc tgggcgttta atgggagcat cggttgttgc agtacttcat
     2521 aatacacctg aatttaccaa aagccttagc gaagccaaaa aagagtttga agaattaaat
     2581 actcctacca atgaactgac cccataaagc tggacagcct gtatcaggct atggagggcc
     2641 catagacaaa tctaccctat atgagcatag gaggagtcta tgggcacacc acgttttacc
     2701 cctgaattta agggattact ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa
     2761 gcttattatg ctgccatcgg aaacgatgat ctggcaacct gagttcacag ataaaacatt
     2821 ctctaggaaa ctcggggcgg ttccgttcac cacatgcaat gtggtgttgc aggggaacgg
     2881 tctgcaccat cccctatatc gatcaatata acaaaaatga caacttcaga ttcagggcac
     2941 aacctaaata tattttaggt cacctctcaa atcgtttgcc tgataccgct ccatttttta
     3001 ataaaaaatc aatcattttt gaatctttgc ttttcattgc attgtcaagc atagtgtccc
     3061 cactatttgc tttttgaaat tttttgttta catcaaccaa tccattattg ataaaatatt
     3121 ctagcacttt atagttggca ccatctttac taagtaagta ttcaacatca tacagattac
     3181 aatatatatt gttgttcatt ttctccatct ctgcgatatc ttgttttata aaattaaatt
     3241 tagatagtaa ataaagtgct atatcagctt ttttat
//
LOCUS       DADLXX010000231         3255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLXX010000231 DADLXX010000000
VERSION     DADLXX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.236"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JNX27_004038"
     CDS             complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JNX27_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7885017.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYES"
     gene            732..1898
                     /gene="nhaA"
                     /locus_tag="JNX27_004039"
     CDS             732..1898
                     /gene="nhaA"
                     /locus_tag="JNX27_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7885018.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1964..2863
                     /gene="nhaR"
                     /locus_tag="JNX27_004040"
     CDS             1964..2863
                     /gene="nhaR"
                     /locus_tag="JNX27_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7885019.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2901..>3255)
                     /locus_tag="JNX27_004041"
     CDS             complement(2901..>3255)
                     /locus_tag="JNX27_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885020.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          765 a          735 c          794 g          961 t
ORIGIN      
        1 gattcgtaag ccgtgaaaac agcaacctcc gtctggccag ttcggatgtg aacctcacag
       61 aggtcttttc tcgtcaccag cgccgccact acggcggtga tacagatgac gatcagggcg
      121 acaatcatcg ccttatgctg cttcattgct ctcttctcct tgaccttacg gtcagtaaga
      181 ggcactctac atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg
      241 ggcttttctc tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg
      301 ctattatatc gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc
      361 gcatttttgt ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat
      421 cgcaattatt gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga
      481 ctgacaacaa attaattatt acttttccta attaatccct caggaatcct caccttaagc
      541 tatgattatc tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca
      601 ccgctgatgg cgcaaattct tcaatagctc gtaaaaaacg aattattcct acactataat
      661 ctgattttaa cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag
      721 agaaataaaa agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta
      781 tacttatcat tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat
      841 ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca
      901 aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc
      961 tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc
     1021 cagttatcgc cgctattggt gggatgattg tgccggcatt actctatctg ccttttaact
     1081 atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg
     1141 cacttggtgt actggcgctg ttgggaagtc gtgttccgtt agcgctgaag atctttttga
     1201 tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta
     1261 atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat
     1321 tgaatctgtg tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga
     1381 ctgcggtgtt gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta
     1441 ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc
     1501 cgtgggtggc gtatctgatt ttgccgctgt ttgcattttc taatgctggc gtttcactgc
     1561 aaggcgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggtttgc
     1621 tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg
     1681 cgcatctgcc tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta
     1741 tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac
     1801 tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat
     1861 acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc
     1921 ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta
     1981 caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga
     2041 ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct
     2101 gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact
     2161 ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa
     2221 ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg
     2281 cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg
     2341 cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat
     2401 catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg
     2461 cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg
     2521 tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct
     2581 taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc
     2641 tttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc
     2701 atatgatttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga
     2761 agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg
     2821 caatacggat tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa
     2881 gttaaggtgg gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt
     2941 tgcccgcttg agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta
     3001 cttccttcat ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc
     3061 gtaccatcat tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac
     3121 tcatttttta atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca
     3181 acgtcactgg gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata
     3241 tgatgaaact gataa
//
LOCUS       DADLXX010000232         3221 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLXX010000232 DADLXX010000000
VERSION     DADLXX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.237"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(35..145)
                     /locus_tag="JNX27_004042"
                     /pseudo
     CDS             complement(35..145)
                     /locus_tag="JNX27_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(469..1146)
                     /gene="ospD1"
                     /locus_tag="JNX27_004043"
     CDS             complement(469..1146)
                     /gene="ospD1"
                     /locus_tag="JNX27_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7885021.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNSHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(1517..1654)
                     /locus_tag="JNX27_004044"
                     /pseudo
     CDS             complement(1517..1654)
                     /locus_tag="JNX27_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1736..2034)
                     /locus_tag="JNX27_004045"
                     /pseudo
     CDS             complement(1736..2034)
                     /locus_tag="JNX27_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2623..2782)
                     /locus_tag="JNX27_004046"
                     /pseudo
     CDS             complement(2623..2782)
                     /locus_tag="JNX27_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2786..3221)
                     /locus_tag="JNX27_004047"
                     /pseudo
     CDS             complement(2786..3221)
                     /locus_tag="JNX27_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          849 a          703 c          616 g         1053 t
ORIGIN      
        1 agtatgatcc tacccacgta atatgaacac aggcttaagt gaggttctgg ttttcaaatt
       61 gttcaggact gagacctcca caccaactgt gccgtcgtca gcgattgtaa tcgcacttga
      121 tataattaaa cactgtatat ttcatttgtg gagtctccag tctgaggggg ctttatgcat
      181 tccgtattgg gcactatgat gccagaaatc tgcaaggctc cacaccttcc tgtatttccc
      241 ttcctctatc acagtgcact ttacttaata gggtgggatg tgttcattac atttattgtg
      301 ttttttatga ttgtttttgc gtccagtgtc gttcgtcatg ggaaactggt tctatgggta
      361 gtatcgctca gtctagttaa ctgtttgttt ctttttagaa attaagcaga tcacacaatt
      421 cggggagaga tccttctaag tgcaattctt tgcagctatt tagcacactt aaaacaactc
      481 ttgtattttg tcagaaataa tattaaacat gtcttgttca ttattgttaa gtctgtctgc
      541 tatttctgta agttttatcc cactatgatg gcatatattt atatatgcca tcatagcctc
      601 gatattccct ttccctgcgg ccagaaataa tcctggtaac ccctcattat ctcttacatc
      661 caatagttta atcgtttctt ttggtgataa atcaaaagtt ttaataaaat ctccataagc
      721 ttgaatcgca gcggaatgac cttcttgaag agctatgaac aaagcagatt ccccagcatt
      781 atttcgtgct gcaagaactt ctagtttttc ctctttgggt atttcggctg ccaacagcga
      841 ttcaccgtat ttacgcgctg catcggcatt tccatgccaa atcaatgaaa acaaattatg
      901 gctgttatac tccttattat tctttgtcgg aatggatatg ctctgtattt tggccaattt
      961 tctgaaagaa acaccactat atcccccccc tgatttttct tcattgatgg cgtgggatat
     1021 agcggtattt gtcacgttaa tgatattatt tttcattcct ttattttcat tagcagtatt
     1081 gctgcctatt attaggtgca tatttttgtt gtttgctgga tgtaatccat agttatttat
     1141 tgacattttt gttcatcctt tttgctaaaa aaaacaataa cctatttttg taatgtaata
     1201 gatattagtc tattgctaat ctaaaaaaac cattgccaca ttgaatggct atattcacca
     1261 ataaattctt tcgatttcgt atattgacat atcgtagata tatcttatcc ctaagattca
     1321 ataatcgagt ccgtttacca ttctgtgcaa gcgccttcac tcagaagtga ccgtcaaggc
     1381 gatcaccaaa cttgataata aagcagctca ttgccatacg ccagtcctta aggggcgttg
     1441 tccatttctg tgatgctgcc tagatttaac tgccagctcc ggcttatcta catctaaata
     1501 tgtgagccta ataccgctac actttgtcca gactatgttt ttctccggga agtgggctaa
     1561 ccgtctccat aaagtgacga acttttttca acagtggcca tattgaacgg cactgatgat
     1621 tacgggttat tgtctcatga agagccggcc acatcactga cagcagtgac ttcaggcctt
     1681 cagcaccaga cagcctgaac cagttaatcc atcgcccgac agatttagta atctagggct
     1741 ggtttttctt cagctgttgc ggttcgaaag tgccattacg atcacgcaga gtacggattt
     1801 ccagaggttc atctccagta gtgataatct tcgttaagca accattgcgg gaattgctgc
     1861 cagatctaag ctgatttttc tcgcacccaa gatgatgagt catattagca ttgagtacgg
     1921 tttcattttc agcatccaat caaactgact gagatcttca ggatttttga gacttttggc
     1981 tagttcatta gctaaagccc gcaacttatt agatgtcaac tgtttttcat tcataaattc
     2041 accttcattt gatatcaaat taaatagttc aacatcagag aattatacaa acttatgtac
     2101 aggctcctat gatcttcgaa tcctgaagat tatagtgaaa ataagttttc acgttgaaat
     2161 cccacatagt tagtatatga taaataatca tggatgacct gattaacctc ttggggtagt
     2221 gcttttatag aagaataaat attctactaa tttaatacat cacctaaaat agtattgttt
     2281 tttatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata ttccagtgcc
     2341 agatcttact tataataaag gggcttggca gtttggataa actattgttc ttattccact
     2401 aatacaaact ttttgtaaag tatattaaat atacttcatt tcatcctgaa atccccggaa
     2461 gtattcaatc aaacaattac accataaaag gaagaggggg ccctaattaa gttgtcaagc
     2521 atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca tataaaacgt
     2581 ctttattgat cctacccaca taatatggac acagtcgtaa gtgaggctct ggttttcaaa
     2641 ttgttccgga ctgagaccac cacaccaacg gtgccgtcgc cagcgattgt aatcgcactc
     2701 gatataacta aacactgttg cccgcactat ttcccgatgg ataatagtgt tctccgtgga
     2761 gacattccat tttcagcgaa tgaattttca tctgttgctg tatccagtca tacagtgact
     2821 gcatcagtgg cgcggctctg gcttttcttg ccgccagacg ctgttctgct gaacatcccc
     2881 ggacttctgc ctcgatggca tacagttcac cgatacgctg cagggcttcc gtggtgatgt
     2941 cggttggcac tcttgcatgc acatcgtgga tttttctccg gacatgagcc atacatgcgg
     3001 cttccgttat tctgccggat tcgtacaacg cccggtaacc accgtaagca tcggcctgaa
     3061 gcactccgct gtaaccggcc agatgattct gtggatggat acctttccgg tccggtgagt
     3121 acgcgaacca gaccgccgga ggcatttccg aaccggcgtt acggtcatca cgaacgtaga
     3181 cccacagccg ggcagtcctt gttttaccgc tgcccggctc c
//
LOCUS       DADLXX010000233         3194 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLXX010000233 DADLXX010000000
VERSION     DADLXX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.238"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(408..605)
                     /locus_tag="JNX27_004048"
     CDS             complement(408..605)
                     /locus_tag="JNX27_004048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885022.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
     gene            1451..2500
                     /locus_tag="JNX27_004049"
     CDS             1451..2500
                     /locus_tag="JNX27_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7885023.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
BASE COUNT         1038 a          561 c          538 g         1057 t
ORIGIN      
        1 tttttatcat cattcaatga acagatagct gtagtcaatg tattattatg caccacactg
       61 ttaatttttt gcaaaacaaa caattacaat acaaaaccat tattaaatgc gatggggatt
      121 tcactattag ttattattta tattgcaact tgcacaggaa ataaagtcag gtattatgca
      181 gagatagaac attggtttaa agagtatggt aattttaata taatccaaag atctatgctt
      241 ggacttaact tatatgcaga tatgttattt tctgtaaaat caattatccc agcattacta
      301 gctttttcca gtactattat atgcaagaaa aaaacaaaaa tactccctct aatttcttgc
      361 tgtatcttga tcacactata tattatacat acaccgccag ttatttttca agccatacat
      421 ttttctgaaa gcaatctttt ctcgacactc tccgtgttta gggtgtcttt tgccatgata
      481 cttacagcac tcattatatt tccaaccgta atatcactta attttaatgt cacttcaatt
      541 ttcatatcca caatgattat tggaaccatt gccacaacat ctatgttagg attatcacca
      601 agcatatatg cttctggtaa tagaatttat tttattccat atttgcttct tataacagca
      661 attgttgttt caaccccaat cgcaataaat aacatagtag caaattttgc caagagccat
      721 tataaaattt gaattaaatt taaaagccac ctcctttttt tggggggggc ttagaaataa
      781 aaacaaattt aattggactt ttattggtta taacacattc ctctatactg ctcatcaccc
      841 gactatacta ggattttcgt cattttttgc tgaaccgctc tcatgaaatc ctgacaacaa
      901 agtcatgttt cacgtcagga tacttcttct ggcatggaaa tataccgtgt gcagctgatg
      961 catatttata tttcaatata atacgatatt gaatggccta caccactggt agttcaggcc
     1021 aaatgacatc cgacggtttc acgatgaact acggcaaaag aatatcagag ccctcattcc
     1081 tccccgaaag ggagcgggct ctggtctggt gagtatgcag atcgtaaagg ctatggccat
     1141 ggtgcgagag tggcagctat gccagaaaga gtgtgtattg cctgagactc taacctgatg
     1201 cagagacgat tgtcttgaac ctgatttatt caacaaagcc gctggcagga tgttacgaaa
     1261 gttatcgcgg ccgcaggctg atttgatatc gatactatcg gtggtactgc ggaagtaaat
     1321 gcctatatag tgcgactttg aaaaataata aattaatatt gaactcgttg attaataatc
     1381 ataaactttc aggcattcat atttgcaatg agtgtttgaa aatcacacaa aactggagag
     1441 cctactttaa atgtataata acaaaatcga caacaacaga aaaacaaggc atgattgggt
     1501 tgatgcttta aaatttctcg gtatttttgc tatatattta ggtcaccttg gtttgggggc
     1561 tggcaaattg tatccttttg tttttagtta tcacgtccca ctattctttt tcgctgctgg
     1621 tttttttact atcaaaaaaa atgacttatc tattttcgat tatatcaagt caaagtttta
     1681 cagattaatg attccatatt tcacttttgc atttactata ttaataataa acacaattaa
     1741 ctcaggtgag acgattgatt atatatatag ccacatttat gatataatat atggtgttag
     1801 aaacaaccaa tttgtaggaa caatatggtt tataaactgc ctttttgtta taatagcaat
     1861 tgatgcaatc tttaaagaaa tagtcaagaa caatatagtg attttaatca tatcactatt
     1921 atcgttcatg ttgtcacaaa cagtgctcaa tcataaccca ttactagatc ctcaatggtt
     1981 ttggaacata gactccgcta tggcatactg gtggttattg cctctcggta gatgtatgtt
     2041 tttagaatta acacgtgaca ggttttttgg aaaatctaaa attggtttca ttgtcttctc
     2101 aattaccgcg ataatgagtg cgtatcaact ccttaaccag aaacctttgc tttttaagat
     2161 tatctcaata ttcaatgcag acataatatc aagtagttat attcaagcta ttaatactat
     2221 tatcaccacg gtaggtttga taatatttaa tatttttata gcaaaaataa tttgtgggaa
     2281 tgactttatt gtaagagctg gaagaaatac tcttaacata tgtggaatgg agtatatcac
     2341 caaattattt atccctatgg ctttagcaat cataggcttc agcgtgacca ttccaaatcc
     2401 tatctgcgca atcatatata cctgtatttg tgtatatgta tcagataaaa ttggccattg
     2461 gctatcgagg acagtcggtg ggccattttt gataaaatga taaaaaggcg caattaattg
     2521 cgccttttta tagatatata gcgcatattt atgctcagat tatactgtta aaattaatgc
     2581 gcaaatgtta cggttttctt tcctagcaac tgaagggaat gttcgaagtg caaacataaa
     2641 attacccctt cactgaacag atattcagtc ggccaacaca tgagaaagaa atacaccaga
     2701 tgaattaaaa tatcatatac ttattaatca aagaaataaa aacataaaca gtaggtggtg
     2761 acgctgataa actggctata aacaacctgg aaggtgatga gctatcgcaa tataatttat
     2821 tggtgaatta tttgaagccg attgataaca ccagtgctca aaagactccc cccggtaacc
     2881 ataagtagct aatatctagt acagtttcga tatctattat cgttactgcg tcgatatagt
     2941 ctaaatagct gcacctaatt ctgtcacact tgtcgtttac agatgggtaa tgtgttgcgt
     3001 atgggcaaca aaactcaagc cagatataac acaacctagc tgagaacgcc ttcctgatgc
     3061 ttcgcccagc aagggctgag cgaaagtgga gcacttttct gcgcagctat ttaggacagt
     3121 attgagtcat attgtttatg tatgagttaa gtttcgtaat atcaattaaa taaaataagt
     3181 tgaaatcatt gtag
//
LOCUS       DADLXX010000234         3171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLXX010000234 DADLXX010000000
VERSION     DADLXX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.239"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..966
                     /locus_tag="JNX27_004050"
     CDS             <1..966
                     /locus_tag="JNX27_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7885024.1"
                     /translation="LSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVM
                     NAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGK
                     SAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIA
                     RDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQA
                     EEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQERDRAAITEREASLPES
                     VLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTPGGD"
     gene            986..1732
                     /gene="traX"
                     /locus_tag="JNX27_004051"
     CDS             986..1732
                     /gene="traX"
                     /locus_tag="JNX27_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7885025.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            1787..2347
                     /locus_tag="JNX27_004052"
     CDS             1787..2347
                     /locus_tag="JNX27_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7885026.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            2478..2690
                     /locus_tag="JNX27_004053"
     CDS             2478..2690
                     /locus_tag="JNX27_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7885027.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            2874..>3171
                     /locus_tag="JNX27_004054"
     CDS             2874..>3171
                     /locus_tag="JNX27_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY7885028.1"
                     /translation="MLHYSGGLKYRWHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          759 a          748 c         1017 g          647 t
ORIGIN      
        1 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
       61 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
      121 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
      181 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
      241 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
      301 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
      361 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
      421 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
      481 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
      541 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
      601 ggagccggaa atggcgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
      661 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
      721 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cggtcaggag
      781 cgtgaccggg ctgccataac tgaacgggaa gcctcgctgc cggagagtgt actgcgtgaa
      841 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
      901 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
      961 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     1021 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     1081 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     1141 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     1201 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     1261 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     1321 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     1381 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     1441 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     1501 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     1561 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     1621 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     1681 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     1741 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga tagagcagaa
     1801 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     1861 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     1921 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     1981 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     2041 tggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     2101 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     2161 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     2221 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     2281 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     2341 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     2401 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     2461 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     2521 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     2581 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     2641 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     2701 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     2761 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     2821 tcgatttttt atattttgtg attaatttca caaaataagg tgttattcaa tgtgtgctgc
     2881 actattcagg gggtctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     2941 aatatattcc actggtatta tttatctttt catggccggt attgtctgca gatattcatg
     3001 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     3061 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     3121 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca t
//
LOCUS       DADLXX010000235         3142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLXX010000235 DADLXX010000000
VERSION     DADLXX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.240"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNX27_004055"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNX27_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7885029.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="JNX27_004056"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="JNX27_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNX27_004057"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNX27_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY7885030.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNX27_004058"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNX27_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY7885031.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="JNX27_004059"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="JNX27_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          761 g          838 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc tctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccaacgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DADLXX010000236         3136 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLXX010000236 DADLXX010000000
VERSION     DADLXX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.241"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            806..1000
                     /locus_tag="JNX27_004060"
     CDS             806..1000
                     /locus_tag="JNX27_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7885032.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESETGT"
     gene            1386..1621
                     /locus_tag="JNX27_004061"
                     /pseudo
     CDS             1386..1621
                     /locus_tag="JNX27_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1646..1780
                     /locus_tag="JNX27_004062"
     CDS             1646..1780
                     /locus_tag="JNX27_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7885033.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2025..3134)
                     /gene="fepE"
                     /locus_tag="JNX27_004063"
     CDS             complement(2025..3134)
                     /gene="fepE"
                     /locus_tag="JNX27_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7885034.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          928 a          774 c          653 g          781 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccccccctaa aaagcggtgt tggcgcagct
      421 acaacaccgc gccgacaccg cttttttaaa tatcacaaag agagcaagaa gaactaattt
      481 gtcataactc tctatttata aagaaaaatc agaaaaaact tgtttttgcg tggggtgtgg
      541 gcatttcagg catgagaaca gactcgcgtt tttccggagt atcagcggaa ttattgatta
      601 agaaacactc gacgcagagc gaaattttat gcaacagcgg ctgcactgcg cagggattat
      661 ttatcagttg atatgctgtg ccatgatacg tcctaaagaa acaggacagg atgtgcaatt
      721 gtaataccgt cgcccgcaac gtcttacaat taccacctgc tcagggcccc ccccgacacc
      781 cggaactgac ggagaacaac ggaaggtgaa caaacaacaa caaactgcgc tgaatatggc
      841 gcgatgtatc aaaagccaga gcctgacact gcttgaaaaa ctggatgctc tggatgccga
      901 cgaacaggcg gccatgtgca aacgactgca cgaactcgca gaagaactcc agaacagcat
      961 ccagattcgc tttgaggccg aaagtgaaac aggaacataa cgaagctgac ggaggcggtc
     1021 acggctttcc cgtgggcgtt ttcgtcagca gacaagcccg tcagggcgcg tcagggggtt
     1081 ttagcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtatatactg
     1141 gcttaatcat gcggcggcag agcagatggt gagagaacag cgacatatga ggtgtgaaat
     1201 gctgcacaga tacgtaagga gaaaatacag cttccggcgc tcttccgctt cctcgctcac
     1261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg tctgctcacg caaagacgga
     1321 tatacggtta tccacagaat caggggataa caccagaaaa aacatgtgag caaaaaagag
     1381 gccccgaaaa aaggccgcgc cggtggcgtt tttccatagg ctccgccccc ctgacaagca
     1441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactta aagataccag
     1501 gcgtttcccc ctggtggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     1561 gtcattccgc tgttatggcc gcgttcgtct cattccacgc ctgacactca gttccgggta
     1621 ggcagttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttaa cgccattggc
     1741 agcggccact ggtaaacagg atgtgcgaga gatgtattga gagttcttga agtggcagcc
     1801 ttactgaggc tacactagaa ggacagattt ggtgacatgt ctcgcacaag actgttacca
     1861 cggttcagaa gttccccaac ttactgaacc ttcgaaaaac cacctcccca ggtggttttt
     1921 tcgttttcta gagtagaaga ctacggctgt aagggaatga tctcaagaag atcctttgaa
     1981 ctttttataa aagttgctta taaaatgatg catcagcaca acttttactc aatcacactc
     2041 atctttctgg cctccatggc atgacgtaaa agtatatagc cacatgcaag catccctcca
     2101 atgacagaag aaagaattac aattatggca ttacttggcc catcctttct gaccggtaac
     2161 gttgggctca attgatactg aaatggcata aaatcaacat cccccacttt cgccacaacc
     2221 aattgattca caacatattg acgatttcgc aactccccac tcaattccga aacatccttg
     2281 attgattttt tgatgttcaa tttagttgct ataccatcag aaccgagagc cacaggaaaa
     2341 tctgggtcat ccttcataat ctgaccattg ctgtatacag gtttttttat tccagccgca
     2401 ttagcaacct ccagtgaata attgaggcgt cgaatatctg cattcagctg gtttctaatt
     2461 tttatctcat ccagtgcaag aatttctttt tcaacattag ttttaacttc tagtttattt
     2521 cttatatctt ccatcaaatc ccttacaaca agggaagaaa cataattaat atacccagac
     2581 aacacatcgt gagcttcttt acttgttgga gcagtaaaac tcagagtcca tgatacatac
     2641 aatgatgact tgtcgttttt cttactggca ttatcatcaa tcgacttcat atttttactc
     2701 aactcaacaa ttgcacgatg cagttcgaga gcgctaatgt tattcttttt aataatctcc
     2761 ataacataag gagatgatct catatactca ttcaacgaat taacggactg aaatttctta
     2821 ataaacagag caaatgcgct atcacgattg atatcaaaat ccactcccag aacatgaagc
     2881 ttggaaagct ccttatttaa ttcactccat tgaacagact cagcaggagt aaccacagca
     2941 gaactagtcc atttttgtgg caaaacaaaa gaaacaagaa agccaataca agcaaaaaat
     3001 agaatcaccc caacaatttt tatttttgct gcccataaaa cgctaatcaa gccaaaaagg
     3061 tcaatttctt cattaccatt cggcaacatg ttgtaattag aaaaatgttc gtcagagacc
     3121 tttgagattt tcattg
//
LOCUS       DADLXX010000237         3049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLXX010000237 DADLXX010000000
VERSION     DADLXX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.242"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JNX27_004064"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JNX27_004064"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7885035.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JNX27_004065"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JNX27_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7885036.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..>3049)
                     /gene="bcsE"
                     /locus_tag="JNX27_004066"
     CDS             complement(1963..>3049)
                     /gene="bcsE"
                     /locus_tag="JNX27_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7885037.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT          762 a          786 c          806 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DADLXX010000238         3042 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLXX010000238 DADLXX010000000
VERSION     DADLXX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.243"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            222..2090
                     /gene="kup"
                     /locus_tag="JNX27_004067"
     CDS             222..2090
                     /gene="kup"
                     /locus_tag="JNX27_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7885038.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2257..2676
                     /gene="rbsD"
                     /locus_tag="JNX27_004068"
     CDS             2257..2676
                     /gene="rbsD"
                     /locus_tag="JNX27_004068"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7885039.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2684..>3042
                     /locus_tag="JNX27_004069"
     CDS             2684..>3042
                     /locus_tag="JNX27_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7885040.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          688 a          724 c          798 g          832 t
ORIGIN      
        1 agtggtaaag gagaggccct ggaaataccc cacagcccca aaaaagagcc agtcagttgc
       61 ttaactgact ggcattactc aaaggggggg ctttttcttt gagccaggtt aatgtggccg
      121 catttaggag tacgattttg ccgttaatcg tgcatactgt gcgctttttt gtggaccaag
      181 ggactaagca cacatttcat atttcaacga aagactagtc tatgagcact gataataagc
      241 aatcattgcc cgcgattacc ctcgcggcga ttggagttgt ctacggcgat attggtacca
      301 gcccgttata tacacttcgt gaatgtttgt ccggccagtt tggttttggc gttgaacgcg
      361 atgccgtgtt tggcttttta tcgctgatct tctggctgct aatctttgtg gtttccatta
      421 aatatctcac cttcgtgatg cgggcagata acgccggtga gggggggatc ctgacgttga
      481 tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg attatggggc
      541 taatcggcgg cagctttttc tatggtgaag tcgtcataac acccgctatt tcagtgatgt
      601 cagccattga aggtctggaa atcgtcgccc cgcagctgga tacctggata gttcccctct
      661 caattatcgt tctcacttta ttatttatga ttcaaaagca tggcacggct atggtcggta
      721 agctgtttgc gccgatcatg ctgacctggt ttttgattct ggcaggactg gggttacgta
      781 gcattattgc taacccggaa gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct
      841 tccttgaata caaaacggtt tcttttattg cattaggggc agtggtgctg tcgattacgg
      901 gggtcgaggc gctgtatgct gatatggggc actttggtaa gttccctatt cgcctggcgt
      961 ggttcaccgt cgtattacct tccttgaccc ttaattactt cggtcaggga gcgttgttgt
     1021 taaagaaccc ggaagcgatt aagaacccgt tcttcctgtt ggcaccggac tgggcgctga
     1081 tcccactgct gatcatcgcc gcactggcga cggtaattgc ctcgcaggcg gttatctctg
     1141 gcgtcttctc attgacgcgt caggcggtac gtctgggata tttgtcgccg atgcgcatta
     1201 ttcacacctc cgaaatggag tcagggcaaa tctatattcc gtttgtgaac tggatgctct
     1261 atgtcgcggt cgtgattgtg attgtcagct ttgagcactc cagcaacctg gcggcggcgt
     1321 acgggattgc ggtgaccgga accatggtac tgacgtctat tctctcgact accgtggcac
     1381 gtcagaactg gcactggaat aagtattttg ttgcgctgat cctgattgct ttcctttgtg
     1441 tcgatattcc attgttcacc gctaacctcg ataaactgct ctccggcggc tggttgccat
     1501 tgagcctcgg tactgtgatg tttatcgtga tgaccacctg gaagagcgag cgtttccgct
     1561 tgctgcggcg gatgcatgaa catggtaact ctctggaagc gatgattgct tcgctggaga
     1621 aatcaccgcc tgttcgcgtg cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca
     1681 ttccctttgc gctgatgcat aaccttaaac ataacaaggt attgcatgag cgggtgattt
     1741 tgttaactct gcgcaccgaa gacgcgccat atgtccataa cgtccgtcgg gtacagattg
     1801 aacaactgtc gcccactttc tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa
     1861 acgtggaaga agttttccac cgctgcggtc tggaaggatt aagttgccgg atgatggaaa
     1921 cctccttctt tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc
     1981 tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg
     2041 aaatcccgcc aaacagggtt atcgaactgg gtactcaggt cgaaatctaa cgccagacgc
     2101 ctcctttctt cataaggggg cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc
     2161 gaggttgatc acatttccgt aacgtcacga tggttttccc aactcagtca ggattaagct
     2221 gcggatcagc gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac cgttcttaat
     2281 tctgatattt catcggtgat ctcccgtctg ggacataccg atacgctggt ggtgtgtgat
     2341 gctggtttac ccatccccaa aagtacaacg cgtatcgata tggcattaac ccaaggtgta
     2401 ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt
     2461 atcgcggaag agatcaaaca acataatccg caactccacg aaacgttgct cactcacctt
     2521 gagcagctgc aaaaacacca gggaaatacc attgaaattc gttacaccac gcatgaacaa
     2581 ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg ttctccgtat
     2641 gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag cattacttca
     2701 gcttaaaggc atcgataaag ccttcccggg tgtaaaagcc ctctcgggcg cagcgttaaa
     2761 tgtctatccg ggccgtgtga tggcgctggt gggcgaaaac ggcgcgggta aatccaccat
     2821 gatgaaagtg cttactggta tctatactcg cgatgccggc acgcttttat ggctggggaa
     2881 agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga ttatccatca
     2941 ggagctgaac ctgatcccgc agttgaccat tgccgaaaac attttcctcg gtcgtgagtt
     3001 tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat gt
//
LOCUS       DADLXX010000239         2992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLXX010000239 DADLXX010000000
VERSION     DADLXX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.244"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            43..357
                     /locus_tag="JNX27_004070"
     CDS             43..357
                     /locus_tag="JNX27_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885041.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            639..1205
                     /locus_tag="JNX27_004071"
     CDS             639..1205
                     /locus_tag="JNX27_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885042.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1410..1526)
                     /locus_tag="JNX27_004072"
                     /pseudo
     CDS             complement(1410..1526)
                     /locus_tag="JNX27_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1732..2286
                     /locus_tag="JNX27_004073"
                     /pseudo
     CDS             1732..2286
                     /locus_tag="JNX27_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2626..2992
                     /locus_tag="JNX27_004074"
                     /pseudo
     CDS             2626..2992
                     /locus_tag="JNX27_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          849 a          648 c          681 g          814 t
ORIGIN      
        1 ttatagaagc tgtcagcggc tacgtcccag ccaaatcact tcatgctcca gcgccagaga
       61 ggcaaggttg ggtttgctca gctgccggtt gattttgttg cgattgaacc ggacagcgtc
      121 cagggcgtcg gcaaaagagc gaatctcacc aatcgttgtt tcattatccg cattaacgat
      181 agcttcaaga aacgccaagg ttttatcgaa ttcatcagca acagtggctc cgggcatacc
      241 gtgaccgttt atacaaaacg gcgttttcag cgtggagttt tcatgaatag cctcaacacg
      301 aatgtcgtga agccagtgat gtatcggcta cgatcctcag ctgatgccag accataggac
      361 tgacactacg aacagcaatt ttaatgacgt aaatcttcat aactacaact aacagggcat
      421 cattatgaat gatgccggtt attttaccgc tgttggtccc ttcatttagg gactgcccca
      481 agttttttca tactctcttt ctcagagagt ttagtctctg ggattccaga cggtccatca
      541 ttgagccaag ggagtaaaga actcaaagca tttacagaat tgtttgaatc agctaaaata
      601 atgacaatgt tactatttta catgacaaag tgattataat gcttggaaca tcttttaata
      661 attttggaat cagtttatct cacaaaagat atttttcagg aaaggtggat gagattatcc
      721 gctgtacaat ggggaaaagg atagtaaaaa tatcatcaac caaaataaat actagtattt
      781 tgagctctgt ttcagaacaa atcggtgaaa atattacaga ttggaaaaat gatgaaaaaa
      841 aagtctacgt atcccgggtg gtgaaccaat gtattgataa gttttgtgca gaacacagcc
      901 gaaaaatcgg cgataatctc cgtaaacaga tatttaaaca ggttgagaaa gattatcgca
      961 tttctctaga catcaatgct gcacaaagtt ccataaatca tcttgtatca ggaagcagtt
     1021 atttcaaaaa gaaaatggac gaactttgtg aagggatgaa tagaagcgtt aaaaatgata
     1081 caacaagcaa tgttgcaaat cttatttccg atcagttttt tgagaaaaat gtgcaatata
     1141 ttgatctaaa gaaattacgt gggaatatga gtgactatat tacaaattta gaatcgcctt
     1201 tctgatgaaa gaacatcccg agtaaggaaa ctaagaaaag taaataccca ggaattgcct
     1261 gagcggtgct atatttctcc tcgcaagtaa gtcctaatat attgaattgt tgtatagcca
     1321 gtttttgtat tttcctagct catggatgtg ggatccctct cccctttctc tgctcaaagg
     1381 taagcggctc gtcagaatca tattgatatt tactgagaag tcagatcaac tttccaaggc
     1441 aacagatcgc gtacccggtt tgccggtcag tcctggatat gttcaataac gtaacgcagc
     1501 catttttctg gctccacatt gttcagacgg catgtgccga tcagcgagaa caacaccgct
     1561 gcatgttcgt caccgctgtc ggaacctgcg agtcgagtca gccggagaga gtttcactct
     1621 ccggctgctt acggaaccgt acgtgacact ctcgcgtcat acggctcttg atcactcatt
     1681 cgatgcacgt agtaaatacc agtgagcaaa ttgatcttac ccagcaatac ggtcagttac
     1741 cgcgcacatg gcctgcctgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
     1801 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagg ctggctgtat
     1861 ctggctgtgg tcattgacct gtggtcacgt gccgttattg gctggtccat gtcaccacgc
     1921 ctgacggcgc aactggcctg cgatgccctg cagatggcgc tgtggcggcg taagagaccc
     1981 cggaacgtta tcgttcactc ggaccgagga agtcagtact gttcagcaga ttatcaggcg
     2041 ctgctgaagt ggcataatct gcgtggcagt atgagcgcaa agggctgctg ctacgataat
     2101 gcctgcgtgg aaagcttctt tcattcgctg aaagtggaat gtctccacgg agaacacttt
     2161 atcagccggg aaataatgcg ggcaacagtg tttaattata tcgaatgtga ttacaatcgc
     2221 tggcgacggc acagttggtg tggcggcctc agtccggaac aatttgaaaa ccagaacctc
     2281 gcttaggcct gtgtccatat tacgtgggta ggatcactta cgccctgtgg ttgaaacgtg
     2341 agcatattgt tttcagatgc tgttgtcttg cttcagagtg atctgtcctc cggatttacg
     2401 caaagttatc cacaggcact gtgcatttaa aaaacttatc ttttcttctt gattttaaaa
     2461 actcagatcg tcatatcatt atgataaaca taataaaaaa ctctaaacat gagaaggttt
     2521 ttaacgtaag gtgagaggat ttttaagtgt atccatagat taaaaatgac aatgcctcca
     2581 attagttgaa ttaggggtta taatacccgc ttttaaggtt ctttcatggt cacagttaac
     2641 tttcattgcc cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaagga
     2701 catgatcgat tccgttgccg tgactgtcac cgggtgtttc agctcactta ctgttacgag
     2761 gcccgtaaac cgggagttaa aaaccaaatc actgaaatgg ctttcaacgg tgccggagtt
     2821 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
     2881 tcccgccccg gcggataaca tctgcgccag tcgctcacgc tgatgttgcc cttatctgtg
     2941 aacttgatgg gcagtggagc ttcgttggca gcaaggcgca acaacactgt ca
//
LOCUS       DADLXX010000240         2871 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.245, whole genome shotgun sequence.
ACCESSION   DADLXX010000240 DADLXX010000000
VERSION     DADLXX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.245"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..216
                     /locus_tag="JNX27_004075"
     CDS             <1..216
                     /locus_tag="JNX27_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7885043.1"
                     /translation="LRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSH
                     DARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            456..716
                     /locus_tag="JNX27_004076"
     CDS             456..716
                     /locus_tag="JNX27_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7885044.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            940..1014
                     /locus_tag="JNX27_004077"
     CDS             940..1014
                     /locus_tag="JNX27_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7885045.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1007..1864
                     /gene="repA"
                     /locus_tag="JNX27_004078"
     CDS             1007..1864
                     /gene="repA"
                     /locus_tag="JNX27_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7885046.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2564..2755
                     /locus_tag="JNX27_004079"
     CDS             2564..2755
                     /locus_tag="JNX27_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885047.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          760 a          747 c          713 g          651 t
ORIGIN      
        1 ctgcgttcgt tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg
       61 gaacgcagac gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg
      121 ctcagaagcc acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca
      181 ggggctgatc agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc
      241 aaccccggaa acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat aactgaaaac
      301 catactattt cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc
      361 aacacagatc ttcgccacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg
      421 cgaaacgatc cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga
      481 cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga
      541 aacaaatggc cgctgttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca
      601 aaaatcctct gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc
      661 agttcgttga gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac
      721 tttactttgt ggcgtagcat gctagattac tgatcgttta aggaatttta tggctggcca
      781 cgccataagg tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcaaaa
      841 accccgataa tcttctttaa ctttggcgag tcagaaagat taccggggcc cacttaaacc
      901 gtatagccaa caatcaagct atgcggggag tatagttata tgcccggaaa agttcaagac
      961 ttctttctgt gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca
     1021 acaaacttat taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc
     1081 ccggacgctt ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt
     1141 tgatttcgcc attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc
     1201 ggtactacgt cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc actatgaccc
     1261 actggccaac cgcgtccagt gttccatcac cacgctggcc attgagtgcg gactggcgac
     1321 ggagtctgct gccggaaaac tctccatcac ccgggccacc cgggccctga cgttccttgc
     1381 agagctggga ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc
     1441 gaccgatatc acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagt
     1501 ggccgccgcg cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct
     1561 ggataccctg ggtatggatg aactgatggc gaaagcctgg cgttttgttc gtgagcgttt
     1621 ccgcagttac cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga
     1681 tgcggacagg cagcgtcagg atattgtcac cctggtgaaa cggcaactga cgcgtgaaat
     1741 ctcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt
     1801 gaaggagcgc atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc
     1861 ctgaaagtga cctcctcaga ataatccggc acgcgccgga ggcattcgca cgcctgaagc
     1921 ccgccagtgc acaaaaaatc agcgctacat acaaaaaaca acctcatcat ccaccttctg
     1981 gtgcatccgg tcccccctgt ttttgataca aaacacgccc cacagacggg gaattttgct
     2041 tatccacatt aaactgcaag ggacttcatc gtaaggttac aaccgttcac gtcataaagc
     2101 gccagccgtc aaccctacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt
     2161 aaactactta attacattca tttaaaaaga aaacctgttc actgcctgtc ctgtggacag
     2221 acagatatgc acctcccacc gcaagcggca ggccccaacc gaagccggtt tagttacaac
     2281 atgcaaacat aaacccctaa aaaacccaag tccaacgcag aactgaaacc acaacgcccc
     2341 tcctcataac tgaaaagcgc cgccgccccg cccgaagggc gggaacagag tcgcttttaa
     2401 ttatgaatgt tgtaactaca tcatcatcgc tgtcagtctt ctgcctggac gtatcgagta
     2461 cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa
     2521 aaccctcgtc gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta
     2581 tggcttagca gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc
     2641 gggcttcggc ggttttactc cggtatcaca tgtaaccaca gcgtgtcgcc ttccatgccg
     2701 ctggcgcggc atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat
     2761 attaagtggt cgtttcaggg atatcgttaa gacttgagcg ataggaatta aaaccccaaa
     2821 aagattaaaa aaacaccaca aaacggatgt ttcttcaaca ccacttttgc t
//
LOCUS       DADLXX010000241         2809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLXX010000241 DADLXX010000000
VERSION     DADLXX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.246"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(675..749)
                     /locus_tag="JNX27_004080"
     tRNA            complement(675..749)
                     /locus_tag="JNX27_004080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Gly,seq:tcc)
     gene            complement(753..828)
                     /locus_tag="JNX27_004081"
     tRNA            complement(753..828)
                     /locus_tag="JNX27_004081"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(793..795),aa:Thr,seq:tgt)
     gene            complement(834..907)
                     /locus_tag="JNX27_004082"
     tRNA            complement(834..907)
                     /locus_tag="JNX27_004082"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(871..873),aa:Arg,seq:tct)
     gene            complement(909..984)
                     /locus_tag="JNX27_004083"
     tRNA            complement(909..984)
                     /locus_tag="JNX27_004083"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Ile,seq:cat)
     gene            complement(1161..1877)
                     /locus_tag="JNX27_004084"
     CDS             complement(1161..1877)
                     /locus_tag="JNX27_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY7885048.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQVGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFV
                     MRTRLEQKAAFSKQSIAKVD"
     gene            complement(1874..2239)
                     /locus_tag="JNX27_004085"
     CDS             complement(1874..2239)
                     /locus_tag="JNX27_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7885049.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            complement(2240..>2809)
                     /locus_tag="JNX27_004086"
     CDS             complement(2240..>2809)
                     /locus_tag="JNX27_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7885050.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTSTSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
BASE COUNT          707 a          758 c          605 g          739 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacacatac cgagcgctgt acaaaaaacc gccagccaag gcagttaaga
      421 gtgtgtcccc ggggtttgct tagaatattt tgtaagttgt ccgaagagat atttacaaca
      481 ccagaatgat gaatcatcag tcccctgcca gaggttcact acgtaacctg accgacaatg
      541 caccattctg gtgctgaaaa aagagcactg gaactgcaac aaaacaacat cacagaacag
      601 gagatggtaa ggagtcagac attcccacac aatattgtgt cagtgcaaat aacaaccttc
      661 gtctcaaatc tcgctggagc gggcagcggg aatcgaaccc gcatcatcag cctggaaggc
      721 tgaggtaata gccattatac gatgcccgca tatggtgccg actaccggaa tcgaactggt
      781 gacctactga ttacaagtca gttgctctgc ctgctgagct aagtcggcgc aggcccacca
      841 ccgaggactt gaacctcgga ccgtcaactt agaaggttga tgctctatcc ggatgagcta
      901 gtggtggttg gtggcccttg ctggacttga accagcgacc tggcgattat gagtcgctcg
      961 ctctcaccac tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc
     1021 aaactcatct gaccaccagc acgtttaacg tcctgtgcca tttttcaggc ataaaaaaac
     1081 cgctcggcgg cgggttttgc ttactttacc atcgcgtaca aaatcggcaa agtatcagat
     1141 ttgcatgaaa tatatgcctt ttaatctact tttgcaatac tttgctttga aaatgccgct
     1201 ttttgttcta aacgtgttct catcacaaac aataaagcct cactatccag tcggtgaaaa
     1261 atgtgtttca ttacaaccca gtgaccagta aatgttttgg accagttttt ggttgttact
     1321 cccgccagta atgccagctc ctgatattca taaccttccc caccaacctg gtattcataa
     1381 ccttccccac caaaaagttc tgcttttact gcctgcgccg ccagccagat taattttttc
     1441 aggcgttcct gcgttttccc tgcaattttt ctggtaccgg attgagcatt aaattcattc
     1501 cacgcccact gtgttatcgc gatctgatat tcccaacaaa tactcccgct gtaacaccac
     1561 aacaaccagg ctttatgatg ttcttcaaga gacagaacag cccgccgcca cgatgatgtc
     1621 gaaaactcaa ccggactgac cagaggaatt gacgtcccct tcgccagcga ttgctttccc
     1681 gggattggtg gattatcccg cgttatcatt tttccagtca cttcatcgcg gtaccggatt
     1741 tttttacgcc tgtaacgccc tgtatcgaac atggcattct cttgccaggc ttcaagctga
     1801 ccttttgttg ccccactcaa atcagcggtg gcgataatga gctgctcacg cacaaactgt
     1861 aaatactggt tattcatgcg caccccagtt ctgtgatttt tatccccagc cgcccaccag
     1921 gaacgagctg accgcgcaca atattgattt catcaaactg ctcgtcgtct ataagtagtc
     1981 cagcatgcgt cagcgcatcc agcggtgcct tcaggatatt gtccaggtca cgacggcgct
     2041 tatccggtgg ctctgcaata atctttatcg ccagccttcc ggacaggttt aatttcagtc
     2101 gctgctggcg aacaattagc gccacatcac ggcgataacg ctcaccggct tttgatacaa
     2161 aatatgtgct gccacgacgt cgccagtagg tgttcaccgt tggcgggtaa ggcaaaacaa
     2221 attctatgcg ttcggtcatt tatgctttcc acttcagaac acccgaattt ctcgcgtgca
     2281 ttaaaaaacg aatcagcaac aacaactgac taccgtgttt ttcttcaaaa tcttttaccc
     2341 cggcgtgtag ttcgttatgg catttacgac acagcggaat aacaaacaaa tcgtcagcct
     2401 ttgttcccat ccctcccagt ccatgaccaa tgatgtggtg cggatcatct gcctgattgc
     2461 cacacgtcat gcatttctgc gtttttaccc agcgcgtgta tacaggcatc tcttcccgtt
     2521 gtggtttctg gcgctggaga tactgagccg gagattccgg atcaacggca atgctgacca
     2581 ccgtctcttc ctgtggtgga ttctgttgct ggtgggcgtg aggcagcggc gcaagatttt
     2641 ttgtgcgctg cttcagtatg ctggtggagg tctgctctcc cggcacgatg tcgctttcac
     2701 ggtacattga gcggattttt tccgcacgca accccagcga acgacgtaat accgcttccg
     2761 gtagcgcgtc cgccacctga ttgcggaccg cccaccagga taattcagc
//
LOCUS       DADLXX010000242         2789 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLXX010000242 DADLXX010000000
VERSION     DADLXX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.247"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            23..985
                     /gene="yeeE"
                     /locus_tag="JNX27_004087"
     CDS             23..985
                     /gene="yeeE"
                     /locus_tag="JNX27_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7885051.1"
                     /translation="MYIAKNNRMFYALLIAISAQSVGVFALIQAGLLTYEAGAFPWLG
                     TVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAVMRSPHASGLNQT
                     LQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKVATLPPRRTGIAHI
                     LFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANILQFLVAGDVKYINW
                     GVFLVLGIFVGAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGNGL
                     VMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            999..1226
                     /locus_tag="JNX27_004088"
     CDS             999..1226
                     /locus_tag="JNX27_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7885052.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNX27_004089"
     CDS             complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNX27_004089"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7885053.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          643 a          660 c          742 g          744 t
ORIGIN      
        1 cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt
       61 gctgattgca atttcggcac aaagcgtggg ggtttttgcg ttaattcagg cgggcctact
      121 gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt atcttttcgg
      181 gctgggaatt gttctggcgg gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg
      241 attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc
      301 tccacacgcc agtggtttaa atcaaacctt gcagcactac actactgaac ataactctat
      361 tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg tgataacgct
      421 ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg
      481 ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg tcactgctgt
      541 actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt
      601 tgggttagga gtcacttccc caacggccaa tattctgcaa tttctggtcg cgggtgacgt
      661 gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggg catttattgc
      721 agccaaagcg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa cattacgtag
      781 cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg
      841 taatggactg gttatgactg caatgatgac ctggcagggc tggattggcc ttgtatttat
      901 gattctcgga gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg
      961 actggcaact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa aagctggatg
     1021 tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg gcagaaatgg
     1081 ccagcggcga tgagttggta attgagtttg attgtaccca ggcaactgaa gcaatcccac
     1141 agtgggcggc agaagaagga catgccatca ccgattatca gcagattggc gatgcagcgt
     1201 ggagcatcac cgttcaaaaa gcctgactca acattgtcct ccgccgtacc agcggcggcg
     1261 gcttcaaatt agacgatctc ttccgcgtac tgccaaagtg cttttaacag cgccactttc
     1321 tctttgtcat cggcatattg ttgtaccagc atctgcaatt catcagcata accctgcaaa
     1381 aactctggcg tgaagacctg gtggcggtgc tcctgccagc gttgctgctc ggcataatcc
     1441 agcgtccccg ggaagttgcg tgcccgataa ttgaacaaca acttttcaat tcgtttatca
     1501 acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac aattttcatt
     1561 gctgcacgat cggcgtcact gaaaaagcca ttataaagct gtgcatccac attatctgaa
     1621 ggtgtaaacg gttcggcttc cgcgaatatc gctaccactt tttcacgcac ttgcggattt
     1681 tcacgcagaa tttttaggtt atcgaggcaa tgctgacgat taattcccag tcggtcggca
     1741 tcttccgggc gtagcgtatt cgcctgggcc agtaccggac atttattgat atgcaccagc
     1801 ttaaccggaa cggcggcgtt atcgccaaga tcggcttttg cggtatataa acgctcgcgc
     1861 aatgtgtcga tatccagctc cagtaatggc gaaatgtctc ctgccaaatc cgccataatt
     1921 acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt attgccacgc
     1981 catgccccaa acattccgga aacgtgagcc aggggtttca tctgcggaac atcaatcaac
     2041 gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg tggctgacgc
     2101 gtttttacca gtttcgccat cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg
     2161 ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg
     2221 tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc acgcataaca
     2281 tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa
     2341 ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga
     2401 atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt
     2461 tctcctttcg cccgtgcctc ctgcggggta ataccggtaa ttaatactgc tccaggccgg
     2521 ggtaaatagt catccgcggg cttgcagtaa aagacttcgg gttcgccgat gacattgaat
     2581 tcgtcatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca
     2641 aaggtttcgt aatcgtgaaa gaggaaggta ggttgtttat ctgtgtctgt cattgtctag
     2701 cattgtccac tgtagttaat taactgtctg ttataactcg aaaagcaacc cttttttcac
     2761 ctgagtatgt ctacgaacgt caaatatag
//
LOCUS       DADLXX010000243         2648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLXX010000243 DADLXX010000000
VERSION     DADLXX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.248"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1475
                     /locus_tag="JNX27_004090"
     CDS             <1..1475
                     /locus_tag="JNX27_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY7885054.1"
                     /translation="AIGLSIAAGGAALTWYQVAETDLSRTAAAAHEAVSVTERLLDEA
                     QGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLSCSSWEGADPVREF
                     ISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVADNMTLLNLRRPLS
                     LRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPLTLYFHDGALSLLL
                     SVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDASTGEIAGVEVLAR
                     WVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGDGKRWHISFNITQS
                     HIMETGFIAECLTFIDAFTPGSITLTIEPTEREPFDNSDEMRCQLKRIHDAGIALALD
                     DFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIEMANTLKKEIIAEG
                     VETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT          682 a          558 c          618 g          790 t
ORIGIN      
        1 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
       61 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
      121 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
      181 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
      241 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
      301 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
      361 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
      421 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
      481 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
      541 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
      601 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
      661 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
      721 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
      781 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
      841 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
      901 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttaccg cctctgacag
      961 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
     1021 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
     1081 ctatagagcc cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
     1141 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
     1201 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
     1261 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
     1321 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
     1381 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
     1441 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
     1501 cattgaacct gtgagggccg ccccctttat tactcagggg ggctttcaac gttctgaatt
     1561 aagatgtttt atgactcact gagcaggatg tgagttccaa cgtaatctcc gctattttta
     1621 ctgtttttca tttgctgcct catctatccc ttcgccttgc ctctgagttg cgttaaaggc
     1681 tgatgtactg cctttaaccc gttcaataca tagccttttt gtcaatcatc atcccgaaga
     1741 gcgttgcact gtgccttcca tagctgtggt tgtcctctca atctgtcagc ccatttatgg
     1801 aaaatcttaa cgaagcaaca atgagatatg taaaaaaagg gccgatttaa tcattcatta
     1861 agacaggtca ttttttctta ctgtgagggc ggtaagcttt tctgtcggta acaggttggg
     1921 agatgctggt attatgagat ttttctaccg tcctgcggcg gaatatgtga aaaatagaaa
     1981 tgattattat tattgcatgg ttatttttag tttgtgatgg tggagaataa tcctattccc
     2041 gtgaccttgt tttttacatt ttatttaact gcatggtata tgcattttgg acggtgaaag
     2101 caagaagcta ttaggcgcta taggtctttt gacggttttt gaatttcccc tcctgatatc
     2161 tctgttatat ttttgtgaat ttctccaatc caaatcaaag tcccaataag tcatggatgc
     2221 atggttaaaa taactatcat ttgtgtaacc tttggtgctt ataacttttc tttattcttt
     2281 tttcaggatt acctggaggg attgaccaaa aaaacattca ttgaatgata tttttctaat
     2341 taatgaggtt ttctaagctc gttgaaataa aaaatatatt ttaatcatgg atttgcatgc
     2401 gtttctttcg ctttttttac acaagttgac attgaactca gcaactataa gaatagccac
     2461 atcccagacg gtgaccgata aaggaaatac cgttaccata tttaaatgaa ttggaaaata
     2521 tcaccgattt gaagtcgact cgttcgggta attattaata ctgaatcttt aatgcttatt
     2581 tcttaagctt gttaacaacg ggaatgaata atgaaagtgt ccaaaattgc actggcaatg
     2641 ggtcttag
//
LOCUS       DADLXX010000244         2622 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLXX010000244 DADLXX010000000
VERSION     DADLXX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.249"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..104)
                     /locus_tag="JNX27_004091"
     CDS             complement(<1..104)
                     /locus_tag="JNX27_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY7885055.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="JNX27_004092"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="JNX27_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY7885056.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="JNX27_004093"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="JNX27_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY7885057.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="JNX27_004094"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="JNX27_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY7885058.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2423..>2622
                     /locus_tag="JNX27_004095"
     CDS             2423..>2622
                     /locus_tag="JNX27_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY7885059.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSA"
BASE COUNT          768 a          552 c          497 g          805 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcatc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atgcctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gcggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagacttc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac gggcgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gc
//
LOCUS       DADLXX010000245         2561 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLXX010000245 DADLXX010000000
VERSION     DADLXX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.250"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1921)
                     /locus_tag="JNX27_004096"
     rRNA            complement(<1..1921)
                     /locus_tag="JNX27_004096"
                     /product="23S ribosomal RNA"
     gene            complement(2057..>2561)
                     /locus_tag="JNX27_004097"
     rRNA            complement(2057..>2561)
                     /locus_tag="JNX27_004097"
                     /product="16S ribosomal RNA"
BASE COUNT          562 a          753 c          539 g          707 t
ORIGIN      
        1 ggggcttcag ttcagagctt cgcttacgct aacccctctc cttaaccttc cagcaccggg
       61 caggtgtcac cccctatact tcgccttacg gcttcgcaga gagctgtgtt tttgctaaac
      121 agtcgcttgg gcctattcac tgcggctttc cgttaagaaa gcaccccttc tcccgaagtt
      181 acggggtcat tttgccgagt tccttaacca gagttctctc gcacacctta ggattctctc
      241 ctcgcctacc tgtgtcggtt tgcggtacag gcacctttta tctcgctaga agcttttctt
      301 ggcagcgggg aatcaaagac ttcgctccat aaggagcttc cccatcacag ctcagccttt
      361 acgataagcg gatttgccta cttatcagcc taactgcttg gacgtgcaca accaatcgca
      421 cgcttcttct atccttctgc gtccctccat tgctcaaacg ataaagaggt ggtacaggaa
      481 tatcaacctg ttgtccatcg cctacgcctg tcggcctcgg cttaggtcct gactaaccct
      541 gagcggacga gccttcctca ggaaacctta ggcattcggt ggacgggatt ctcacccgtc
      601 tttcgctact cataccggca ttctcacttc taagcactcc accagtcctt ccggtctgac
      661 ttcactgtcc ttagaacgct cccctaccac tgataccatt ggtatcaatc cgcagcttcg
      721 gtggtgtatt tagccccggt acattttcgg cgcagagtca ctcgactagt gagctattac
      781 gcactcttta aatggtggct gcttctaagc caacatccta gttgtctaag caactccaca
      841 tccttttcca cttaatacac actttgggac cttagctggc ggtctgggct gtttcccttt
      901 tgactacgga tcttatcact cgcagtctga ctcctaagga taagtcattg gcattcggag
      961 tttgactgaa ttcggtaatc cgatgaggac ccctagtcca atcagtgctc tacctccaag
     1021 actcttacac ttaaggctag ccctaaagct atttcgggga gaaccagcta tctccaggtt
     1081 cgattggaat ttctccgcta cccacacctc atccccgcac ttttcaacgt gcgtgggttc
     1141 gggcctccat tcagtgttac ctgaacttca ccctggacat gggtagatca cctggtttcg
     1201 ggtctacgac cacgtactaa acgccctatt cagactcgct ttcgctgcgg ctccgcctct
     1261 tcagcttaac ctcgcacggg atcgtaactc gccggttcat tctacaaaag gcacgccatc
     1321 acccgttaac gggctctgac tatttgtagg cacacggttt caggatctct ttcactcccc
     1381 ttccggggtg cttttcacct ttccctcacg gtactggttc actatcgatc actagggagt
     1441 atttagcctt gggagatggt cctcccagat tccgacggaa tttcacgtgt tccgccgtac
     1501 tcaggataca ttcaagagag aacgaagttt cgactacggg gttgttaccc tctacgacgg
     1561 acctttccag gtcgcttcgt ctacctcgtt cctttgtaac tccgtataga atgtcctaca
     1621 accccaagag gcaagcctct tggtttgggc tatgttccgt ttcgctcgcc gctactcagg
     1681 aaatcgcatt tgctttctct tcctccaggt acttagatgt ttcagttccc tgggtctgtc
     1741 ttccttaccc tatgtattca gataaggata ccataccatt acgtatggtg ggtttcccca
     1801 ttcggaaatc ttcggatcaa agcttactta cagctccccg aagcatatcg gcgttagtcc
     1861 cgtccttcat cgactcctag tgtcaaggca tccaccgtgc gccctttcta acttaaccaa
     1921 actaaaaatt aaaaaatatg agctacactg ttatctagtt ttcaaagaac atacatttat
     1981 atatgagaga tagttctctc aaaactgaac aaaacgaaac acggaaactt tattgatgaa
     2041 cagcgttcat caattctcca tagaaaggag gtgatccagc cgcaccttcc gatacggcta
     2101 ccttgttacg acttcacccc aatcatctgt cccaccttag gcggctggct ccaaaaaggt
     2161 taccccaccg acttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaaggc
     2221 ccgggaacgt attcaccgcg gcatgctgat ccgcgattac tagcgattcc agcttcatgt
     2281 aggcgagttg cagcctacaa tccgaactga gaacggtttt atgagattag ctccacctcg
     2341 cggtcttgca gctctttgta ccgtccattg tagcacgtgt gtagcccagg tcataagggg
     2401 catgatgatt tgacgtcatc cccaccttcc tccggtttgt caccggcagt caccttagag
     2461 tgcccaactt aatgatggca actaagatca agggttgcgc tcgttgcggg acttaaccca
     2521 acatctcacg acacgagctg acgacaacca tgcaccacct g
//
LOCUS       DADLXX010000246         2511 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLXX010000246 DADLXX010000000
VERSION     DADLXX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.251"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..79
                     /locus_tag="JNX27_004098"
     CDS             <1..79
                     /locus_tag="JNX27_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HAY7885060.1"
                     /translation="AKQDIEERFDINKTALKILTLAKHK"
     gene            84..1034
                     /gene="virK"
                     /locus_tag="JNX27_004099"
     CDS             84..1034
                     /gene="virK"
                     /locus_tag="JNX27_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7885061.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            1099..2043
                     /gene="lpxM"
                     /locus_tag="JNX27_004100"
                     /gene_synonym="msbB"
     CDS             1099..2043
                     /gene="lpxM"
                     /locus_tag="JNX27_004100"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7885062.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(2197..>2511)
                     /locus_tag="JNX27_004101"
     CDS             complement(2197..>2511)
                     /locus_tag="JNX27_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY7885063.1"
                     /translation="ISLPKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRG
                     IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLA
                     KV"
BASE COUNT          690 a          500 c          581 g          740 t
ORIGIN      
        1 ggcaaaacag gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac
       61 attagcgaag cacaagtaac aatatgtttt ctgtaagtaa cttatcattt atcggtttcc
      121 ttaaaaggat tgttttttcc tcagattcac tcccggggaa gtgggaacac agaaaattcc
      181 ggttcatgta cattttgcga tgtgctataa atccggttgc cagtattcga tattattacg
      241 aactgcgttc cttgcagtgc attgaggata ttctggcaat acaacccacg ttgccagcaa
      301 gaattcatcg accttatttg cataaagggg ggcgagcctg gtcccggggg caatatattc
      361 ttgagcatta tcgctttgtg caaaatttgc cagaaaaata ttctgaattt ttgtttccac
      421 aaaaatcggt atcattagtc caattcatcg ggaaagatgg agaggatttt gatatccagt
      481 gttctcccag tggttttgac cgggaaggag agttgatgtt gtcattgttt ttcaatcaaa
      541 tagtgattgc ccggttaaca ttttcagtca ttctgactca aaatggacac accgctttta
      601 ttggcggatt acagggagcc cccaaaaata ccggacctga tgttattcgg tgcgcaaccc
      661 gggcctgtta tgggcttttc ccaaaacgga ttatttttga agcattttgt gcgttgatga
      721 aagcttgtaa tgtctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat
      781 tgagatactg gtatcagaaa cgcaaaacct ttgttgctgt ctacagtgat ttctgggagt
      841 ctgtagcagg gaaaacctgt ggtgactggt acaaattacc aacacaggtg gttcgaaagc
      901 cgctaagcaa tatagctagt aaaaaacgct ctgagtatcg aaaacgatac gctttgctgg
      961 attatatcca tgaaactgct atccgctctt tggatgcata tcctgtaaac tcagaacatc
     1021 aggacttaaa ttaaggtttg atgtattctc tgaataaaat attaatgatg attttggtag
     1081 gggcattcgc actaaataat gaaaaaatac aaatccgagt ttattcctga atttaagaaa
     1141 aattatcttt cccctgttta ctggtttaca tggttcgttt tgggaatgat tgcaggtatt
     1201 tcaatgtttc ccccttcatt cagagatcct gtcttggcca aaatagggcg ttgggtgggc
     1261 agattgagca gaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccggaa
     1321 aagagtgata cagaacggga aataattgtc gacaatatgt ttgccacagc attgcaatcc
     1381 atagtgatga tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc
     1441 tggaaaggcc tcgaaattct tgaggaaatc cggcacaata acagaaatgt gatttttctg
     1501 gttccccatg gctggagcgt ggatattcct gcaatgttgc tggcagctca gggggaaaaa
     1561 atggccgcca tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattctgta
     1621 cggcgtaaat tcggggggcg cttacattcc cgggaggatg ggataaaacc atttattcag
     1681 tcagtacgcc agggatactg ggggtattac cttccagatc aggatcatgg tcctgaatac
     1741 agtgaatttg ctgatttttt tgcgacctat aaagcgacat taccaattat tggacgtctg
     1801 atgaacatca gtcaggctat gattataccg cttttcccgg tttatgatga aaaaaaacat
     1861 ttcctgacta ttgaagttcg gccaccaatg gatgcatgca ttgccagcgc ggacaataaa
     1921 atgattgccc gacaaatgaa caaaacagtg gaaattttgg tggggtcaca tccggaacag
     1981 tatatctggg ttttaaaatt gttaaaaacg cgcaaatcaa acgaagcgga cccgtaccct
     2041 tgaaattata aagtacaggt atttccacta gttgtttctt acaggttacc aatcgaaaca
     2101 catccccctt ccgtgtgttg cccgtttggc acggaggact gtgtagatgc agttcgttaa
     2161 atttaattta tgcctaaata gctgcgctta ataccgctac acttttgcca gcccatgttt
     2221 gcctccgggg aatgggctga cggtttccat aaaatggcga acttttttca acagttgcca
     2281 cattgagcgg cactgatgat tacgcgttat tgtgtcgtga agtgcctgcc atagccgttc
     2341 aacatgattc acccatggcg agtaaaccgg ctgataaatg cccctgaact tcgggttctc
     2401 cttcagccag ctctgtgttt cccggctttt gtggataatg tagttgtcca cgatcagcgt
     2461 gatggttttt gcccgacggt atgtcgcttt aagccgcttc ggcaggctga t
//
LOCUS       DADLXX010000247         2503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLXX010000247 DADLXX010000000
VERSION     DADLXX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.252"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            155..1108
                     /locus_tag="JNX27_004102"
                     /pseudo
     CDS             155..1108
                     /locus_tag="JNX27_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNX27_004103"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNX27_004103"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7885064.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYSVGQGPMWVVVNAH"
BASE COUNT          692 a          613 c          561 g          637 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgaatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gactggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aataagcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttat tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgccg cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DADLXX010000248         2491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLXX010000248 DADLXX010000000
VERSION     DADLXX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.253"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNX27_004104"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNX27_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7885065.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JNX27_004105"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JNX27_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JNX27_004106"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JNX27_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..2156
                     /locus_tag="JNX27_004107"
     CDS             1905..2156
                     /locus_tag="JNX27_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885066.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(2176..>2491)
                     /locus_tag="JNX27_004108"
     CDS             complement(2176..>2491)
                     /locus_tag="JNX27_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY7885067.1"
                     /translation="ISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRG
                     IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLA
                     KV"
BASE COUNT          702 a          481 c          575 g          733 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata atctaaatag
     2161 ctgcgcttaa taccgctaca cttttgccag cccatgtttg cctccgggga atgggctgac
     2221 ggtttccata aaatggcgaa cttttttcaa cagttgccac attgagcggc actgatgatt
     2281 acgcgttatt gtgtcgtgaa gtgcctgcca tagccgttca acatgattca cccatggcga
     2341 gtaaaccggc tgataaatgc ccctgaactt cgggttctcc ttcagccagc tctgtgtttc
     2401 ccggcttttg tggataatgt agttgtccac gatcagcgtg atggttttcg cccgacggta
     2461 tgtcgcttta agccgcttca gcaggctgat g
//
LOCUS       DADLXX010000249         2477 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLXX010000249 DADLXX010000000
VERSION     DADLXX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.254"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            33..1085
                     /locus_tag="JNX27_004109"
     CDS             33..1085
                     /locus_tag="JNX27_004109"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY7885068.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMCLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1161..>2477
                     /locus_tag="JNX27_004110"
     CDS             1161..>2477
                     /locus_tag="JNX27_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7885069.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSTA
                     FVTTGLGKRIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPDCRIAGLPDCRIAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAG
                     LAELGPMKIREKMLLGVFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVV
                     KNKGGWNTLIWYGGIIGLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSII
                     VRYFFASGSAYIVAMLPVFAMLANVSGAPLMLTALALLFSNSYGGMV"
BASE COUNT          588 a          556 c          639 g          694 t
ORIGIN      
        1 ataagttatg tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc
       61 gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc
      121 agcgcgataa aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta
      181 ttggcggcgg taatccactg accagtaaag tcgccattat tagccgttcc agcgatccgc
      241 gtgctgatgt cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca
      301 cgcctaactg cggcaatatg ctgtctggcg taggggcatt cgccattgaa aatggtttga
      361 ttgcagcgac ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca
      421 tcgaagctga tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg
      481 acggcgtacc gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga
      541 aaaccggaaa agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga
      601 cctgtatcga tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag
      661 gttatgaatt accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta
      721 tccggctaca agcgggtaaa gcaatgtgct taggtgatgt cagtaatatg gttatcccta
      781 aacctgtgct tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc
      841 cgcattcttg ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg
      901 cattggaagg caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata
      961 ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga
     1021 cgttacgcgc atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc
     1081 cctgaaaaaa ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata
     1141 cagaaagaac aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag
     1201 cgatcccatg tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg
     1261 tgctttttgg tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac
     1321 ctgtcgtact gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg
     1381 gtgcgtttaa gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt
     1441 tctcggcgtt taccttaagc accgcatttg tgaccaccgg tttaggtaaa cgtattgcct
     1501 atctgctgat tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc
     1561 tcgatctggt actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt
     1621 taccgatcat caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc
     1681 gtgtcggaca ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt
     1741 tctttaccgc aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc
     1801 tgcaaattag ctggggtgga tgggcgctgg ccgccggatt gccggattgc cggattgccg
     1861 gattgccgga ttgccggatt gccggattgc cgggcatcat tatgctgctg gtcaccccgc
     1921 tggtaattta caccatgtat ccaccagaaa ttaaaaaggt ggataacaaa accatcgcta
     1981 aagcgggcct tgccgaactg ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct
     2041 ttgtgctggc gctactgggc tggattttca gtaagtctct gggggttgat gaatccactg
     2101 tggcaatcgt tgttatggcg accatgctgc tgctgggtat cgttacctgg gaagacgtgg
     2161 ttaaaaataa aggcggctgg aataccttaa tctggtacgg cggtattatc ggcttaagct
     2221 ccttattatc gaaagttaaa tttttcgaat ggttagctga agtctttaaa aataacctgg
     2281 catttgatgg tcacggtaac gttgctttct tcgttattat tttcctcagc atcatcgtgc
     2341 gttatttctt cgcttccggt agtgcctata tcgttgctat gttacctgta tttgccatgc
     2401 tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct ggcactgttg ttctccaact
     2461 cctatggcgg catggtt
//
LOCUS       DADLXX010000250         2456 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLXX010000250 DADLXX010000000
VERSION     DADLXX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.255"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            9..2243
                     /gene="xdhA"
                     /locus_tag="JNX27_004111"
                     /pseudo
     CDS             9..2243
                     /gene="xdhA"
                     /locus_tag="JNX27_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            2254..>2456
                     /locus_tag="JNX27_004112"
     CDS             2254..>2456
                     /locus_tag="JNX27_004112"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY7885070.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
BASE COUNT          593 a          649 c          647 g          567 t
ORIGIN      
        1 aatcatgcat gcgcgtcgat tccattgcta aggtcaccgg gcgggcacga tatactgacg
       61 attatgttat ggcgggcatg tgttacgcga aatatgtacg tagccctatc gcacatggtt
      121 atgccgtaag tattaatgat gaacaagcca gaagtttacc gggcgtactg gcgattttta
      181 cttgggaaga tgtgcctgat attccattcg ctacagctgg gcatgcctgg acacttgacg
      241 aaaacaagcg cgataccgcc gatcgcgcac tgctaactcg ccatgttcgt catcatggtg
      301 acgccgttgc catcgtcgtg gcccgcgatg aactcacggc agaaaaagcg gcgcaattgg
      361 tcagcattga gtggcaagaa ttacccgtta tcaccacgcc agaagcggcg ctgacagaag
      421 acgctgcacc aatccataac ggcggcaatt tactgaaaca aagcacgatg tcgacgggca
      481 atgtccaaca aacaatcgat gccgccgact accaggtaca ggggcactat cagactcccg
      541 ttattcaaca ttgtcacatg gaaagcgtaa catcgctggc gtggatggag gatgactcgc
      601 gaattaccat cgtttccagc acccagatcc cgcacattgt tcgccgcgtg gttggtcagg
      661 cgctggatat tccctggtca tgcgtacgag tcatcaaacc gtttgtcggt ggcggttttg
      721 gtaataaaca ggatgtactg gaagagccaa tggcggcatt ccggtgaaag tttcccttag
      781 ccgtgaagag tgtttcctcg caacccgtac ccgccacgct tttactattg acgggcaaat
      841 gggcgtgaac cgcgacggaa ccttgaaagg ttatagtctg gatgttctgt ctaacaccgg
      901 cgcttatgca tctcacgggc actccatcgc ttctacgggg ggggaataaa gtcgcttacc
      961 tttatcctcg ttgtgcctac gcttacagtt caaagacctg ctataccaac ctcccctcgg
     1021 ctggtgcgat gcgtggttat ggcgcgccac aagtcgtatt tgcctttgag tctatgcttg
     1081 atgacgccgc gacagcgtta ggtattgatc ctgttgaaat tcgtttacgc aacgccgccc
     1141 gcgaaggaga tgctaatccg ctcacgggca aacgtatttg cagcgcaggg ttgccggagt
     1201 gtcttgaaaa aggccggaaa atctttgaat gggaaaaacg ccgtgcagaa tgccagaacc
     1261 agcaaggcaa tttgcgccgc ggcgttggcg tcgcctgttt tagctacacc tctaacacct
     1321 ggcctgtcgg cgtagaaata gcaggcgcgc gccttctgat gaatcaggat ggaaccatca
     1381 acgtgcaaag cggcgcgacg gaaatcggtc agggtgccga caccgtcttc tcgcaaatgg
     1441 tggcagaaac cgtgggggtt ccggtcagcg acgttcgcgt tatttcaaca caagataccg
     1501 acgttacgcc gttcgatccc ggcgcatttg cctcacgcca gagctatgtt gccgcgcctg
     1561 cgctgcgcag tgcggcacta ttattaaaag agaaaatcat cgctcacgcc gcagtcatgc
     1621 tacatcagtc agcgatgaat ttgaccctga taaaaggcca tatcgtgctg gttgaacgac
     1681 cggaagagcc gttaatgtcg ttaaaagatt tggcgatgga cgctttctac caccctgaac
     1741 gcggcgggca gctctctgct gaaagctcca tcaaaaccac cactaaccca ccggcgtttg
     1801 gctgtacctt tgttgatctg acggtcgata ttgcgctgtg caaagtcacc atcaaccgca
     1861 tcctcaacgt tcatgattca gggcatattc ttaatccact gctggcagaa ggtcaggtac
     1921 acggcggaat gggaatgggc attggctggg cgctatttga agagatgatc atcgatgcta
     1981 aaagcggcgt ggtccgtaac cccaatctgc tggattacaa aatgccgacc atgccggatc
     2041 tgccacaact ggaaagcgcg ttcgtcgaaa tcaatgagcc gcaatccgca tacggacata
     2101 agtcactggg tgagccaccc ataattcctg ttgccgctgc tattcgtaac gcggtgaaga
     2161 tggctaccgg tgttgcaatc aatacactgc cgctgacgcc aaaacggtta tatgaagagt
     2221 tccatctggc aggattgatt tgaggataac atcatgtttg attttgcttc ttaccatcgc
     2281 gcagcaaccc ttgccgatgc catcaacctg ctggctgaca acccgcaggc caaactgctc
     2341 gccggtggca ctgacgtact gattcagctc caccatcaca atgaccgtta tcgccatatt
     2401 gttgatattc ataatctggc ggagctgcgg ggaattacgc tggcggaaga tggctc
//
LOCUS       DADLXX010000251         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLXX010000251 DADLXX010000000
VERSION     DADLXX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.256"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..565
                     /locus_tag="JNX27_004113"
     CDS             <1..565
                     /locus_tag="JNX27_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7885071.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JNX27_004114"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JNX27_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY7885072.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNX27_004115"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNX27_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7885073.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JNX27_004116"
     CDS             2167..2403
                     /locus_tag="JNX27_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7885074.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          627 a          534 c          626 g          652 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg catgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctatcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADLXX010000252         2436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLXX010000252 DADLXX010000000
VERSION     DADLXX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.257"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1334
                     /locus_tag="JNX27_004117"
     CDS             <1..1334
                     /locus_tag="JNX27_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7885075.1"
                     /translation="VFSITKKHQPYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRI
                     TRTTSGANAGGGNLTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEE
                     IIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTE
                     TDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNA
                     TEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFVE
                     GISDPNDSKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPDC
                     GAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELN
                     WKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            1312..1608
                     /locus_tag="JNX27_004118"
     CDS             1312..1608
                     /locus_tag="JNX27_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7885076.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            1668..>2436
                     /locus_tag="JNX27_004119"
     CDS             1668..>2436
                     /locus_tag="JNX27_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7885077.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKT
                     GIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNF
                     LPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEK"
BASE COUNT          740 a          574 c          602 g          520 t
ORIGIN      
        1 cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct
       61 ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc
      121 gtgtttcacg catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg
      181 atcgcggcga aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg
      241 tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca
      301 ttgaggaaat tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca
      361 ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac
      421 caattgggat cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg
      481 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      541 ccccgatgcc gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac
      601 catctggcgc aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg
      661 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      721 aatatcagga actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc
      781 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      841 acccgaatga ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa
      901 cggaaaatga agtgcgacag actgaagata aacagcacca gaacagcgaa ccggaggagg
      961 gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg gtgatgggag
     1021 acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag gaagaagagc
     1081 cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc gatcagcata
     1141 atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag gtgttgattg
     1201 cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg
     1261 acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct
     1321 tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt
     1381 cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg
     1441 tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc
     1501 cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1561 gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa tctatggcgc
     1621 tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg gggtttgctg
     1681 gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg cttatgcggc
     1741 aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa acaggctctg
     1801 actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca aaaacccgct
     1861 cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc gtaatgatca
     1921 tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga cgccgaaacc
     1981 gtaaacgagg actggaattt tcagactggg cgtggctcaa acaggagtgg cgacgcttta
     2041 ccgatcgatt tgttaacagt caggcgcaca ttattatgtg cgggcgtgca ggatatgagt
     2101 atgacttttt cgaaggtgat gatggcaaac gccagttggc aaaaaccggt ataaaaatga
     2161 aagcagaaac agaaaccggt tatgaaccct ccatcctgat ccagatggag aagcaaatga
     2221 acatagaaac cggtcaggtg tggcgcaccg ccagaatact aaaagatcgc tccacccgta
     2281 ttgatggaca ggtattttca aacccaacat ttaaaaactt tcttcctcat atcgagtttc
     2341 ttaacctggg aggtgaacac ctcggcatcg atacctcacg ggataacagt gaattatttg
     2401 ccaacgacgg tacaccgaca tggctaaaag aaaagc
//
LOCUS       DADLXX010000253         2373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLXX010000253 DADLXX010000000
VERSION     DADLXX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.258"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..823)
                     /locus_tag="JNX27_004120"
     CDS             complement(<1..823)
                     /locus_tag="JNX27_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7885078.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JNX27_004121"
     CDS             complement(1424..1672)
                     /locus_tag="JNX27_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7885079.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JNX27_004122"
     CDS             complement(2027..>2373)
                     /locus_tag="JNX27_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7885080.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          439 c          575 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADLXX010000254         2342 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLXX010000254 DADLXX010000000
VERSION     DADLXX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.259"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..123
                     /locus_tag="JNX27_004123"
     CDS             <1..123
                     /locus_tag="JNX27_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885081.1"
                     /translation="PQPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(256..768)
                     /locus_tag="JNX27_004124"
     CDS             complement(256..768)
                     /locus_tag="JNX27_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7885082.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            963..1727
                     /locus_tag="JNX27_004125"
     CDS             963..1727
                     /locus_tag="JNX27_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885083.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQTLKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIGGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGECDLNDFYSDIQTDAESGFT"
     gene            1678..>2342
                     /locus_tag="JNX27_004126"
     CDS             1678..>2342
                     /locus_tag="JNX27_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7885084.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          663 a          523 c          592 g          564 t
ORIGIN      
        1 cctcagccga aacctccagc gcctccggca tgggcaatga tgccaccgtc gaactctctc
       61 cagttgctgg acgaaacgtt ctcggtatcc gggacggaat catcagcgac cagacagcat
      121 tgagaatgct tcaggagtac atcaggatcc aatgccttgg ggggtagcgg taattttact
      181 cattatcctt caaatcagat tctgttgtca gaggaatggg ggaagctgga ttcgcaagag
      241 ctttttatgg gcagtttagc taaaaaaagg caacacgaag ctaaaactac caacataaag
      301 tgctgaccag ctagcagctg aaatcagact cactgtatac actttcactg gatgaagttg
      361 caacattcct gccaccaaag gagcaatgta acgcagcacg gcgataaagc gcgaagtgaa
      421 aagtattgac acagagttat tttgcaactg aaggcgaacc cgttctattg tattggaacg
      481 gctcgatatt atacatgcaa gttgagggat atgcccaatt attataccaa gatggtaatt
      541 aacgatagta ccgcaccatg cgccactcat gaccgtgatt ccagcttccc atggtgaaag
      601 agtcgtcttg ctgactgtaa ttacagtggc catcataatt gatgctggtg gcaatatcgc
      661 cgaaatgagt aaagtggatt ttgtgaacgc tattataaaa agaagccccc ataggcgata
      721 tggatgaagt acaaaatagt tcatcaaagc atttatccac tccatcaatg tctttccttc
      781 tgtgaatttt tctttgctat ttaaccgtga tacgacttaa tttaggctta aacaccttac
      841 atttacaagg tactcctgtg gggggccctg ccacggagcg tcgcaggagt gagtgatgag
      901 gaagaattaa agcaacctat tgtgacaggc aacgataaca gtaaatcaca ggaagcataa
      961 tcatggcaaa accggactgg gaggccatcg aatcggcata ccgggccgga gtccttagcc
     1021 tccgtgatat aggcgagaaa tacggcgtta cagaaggggc tatcaggaag agggccaaaa
     1081 agcttggttg ggcacgcagt ggcggtacgc aggtttgcaa aaatggtacg caaaaaagga
     1141 aagtgcgtac cagcagaaag cctgccatta ctggccttac acaaaaaagt acgcaactaa
     1201 aaacagaatc tacaccggat acgaaaccga tacgcggaat gcgtaccgat cccccgacta
     1261 acccattcca acccggtaac cagcagacat taaaacacgg tggttatgcc cgtcgccttc
     1321 tgctcaaaga tgaggtgata gaggacgcta aagcgttgac gctcgaggac gaattatttc
     1381 gccttcgtgc taacaacctt gtcgctgcag agaatattgg tcggtggttg gtgtcgctgg
     1441 aagatgctaa tggggaccag gaaaggaaga tgctgattga aaatatcagc gccgccgaga
     1501 aagcaatgat gcgcaataca gttcgtattg agtccatcgg tggcacgctt gcgacggtag
     1561 gaaaaatatt tgctgataca gcctaccgca aggccgccac tgataaggtg tctctggagg
     1621 ctgatcgtct tcgccgtgat gcaggtattg atgatggcaa tggagagtgt gacctcaatg
     1681 acttctactc tgacatccaa accgacgctg aatccggctt tacgtagttt ctggactatg
     1741 cgggcacgta acaaagtgct ttatggtggt cggtcatcgt caaaatcatg ggatgccgct
     1801 ggcattgcca tatttctgtc gaataaatac accctgcgtt tttgttgtgc ccgtcagatc
     1861 cagaataaaa tcgaagagtc ggtgtatacc ctgctcaaaa ttcagataga caggtttggt
     1921 ctgcggcacc gtttccgtat tctgaacaac aaaatcatta accgggttac tggctcggaa
     1981 tttgtttttt atggattatg gcgcaacatc gaagaaatta agtcactgga ggggatcgat
     2041 gtgttgtggc tggaagaagc ccacgcactg acggaatacc agtggaaaat tctggagcca
     2101 acgatccgta aagagggttc ggaatgctgg ttcatattca accccggact tgttactgat
     2161 ttcgtctggc gcaacttcgt tgttgatccg cccgaaggca ctctcatccg caaaattaac
     2221 tatgacgaaa atccgtttct gtctgacacc atgcttaagg ttatcgacgc ggcgcgacgc
     2281 cgtgatccgg atggttttaa acatgtgtat gagggcgttc cggagtctga tgatgatgcg
     2341 gc
//
LOCUS       DADLXX010000255         2301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLXX010000255 DADLXX010000000
VERSION     DADLXX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.260"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..784)
                     /locus_tag="JNX27_004127"
     CDS             complement(<1..784)
                     /locus_tag="JNX27_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7885085.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="JNX27_004128"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="JNX27_004128"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7885086.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..2301)
                     /locus_tag="JNX27_004129"
                     /pseudo
     CDS             complement(2137..2301)
                     /locus_tag="JNX27_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
BASE COUNT          562 a          589 c          576 g          574 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagctaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactctct
     2281 ttcttaatgc tgttggtttc g
//
LOCUS       DADLXX010000256         2280 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLXX010000256 DADLXX010000000
VERSION     DADLXX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.261"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="JNX27_004130"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="JNX27_004130"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7885087.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYKF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          579 a          564 c          673 g          464 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacgctt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tgactggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactggccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggcgc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgcggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat aagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact cagcacatgg
//
LOCUS       DADLXX010000257         2255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLXX010000257 DADLXX010000000
VERSION     DADLXX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.262"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            115..633
                     /locus_tag="JNX27_004131"
     CDS             115..633
                     /locus_tag="JNX27_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7885088.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            640..816
                     /gene="mbeD"
                     /locus_tag="JNX27_004132"
     CDS             640..816
                     /gene="mbeD"
                     /locus_tag="JNX27_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7885089.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQV"
     gene            complement(1175..1642)
                     /locus_tag="JNX27_004133"
     CDS             complement(1175..1642)
                     /locus_tag="JNX27_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885090.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(1970..>2255)
                     /locus_tag="JNX27_004134"
     CDS             complement(1970..>2255)
                     /locus_tag="JNX27_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885091.1"
                     /translation="DAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSL
                     REAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR"
BASE COUNT          598 a          447 c          654 g          556 t
ORIGIN      
        1 atgtgattca ggcgctcaca gaggcaggga ttgacgtggt cagggtgacg cgaagcagta
       61 tcagcattgc agacccgaac ggcgggaaga atgtcagact gaaaggagcg ttttatgagc
      121 aatcttttac agacgggtgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
      181 gagaaaatgc tgaacgacga gttcaggaag ctcgaagaat ctgtaagcaa ggctgtgaca
      241 tcaaacgaga cgaaaatcaa agacgctata gccctgttca cagcttcgac agaggaatca
      301 ctgaaaaaac accgggaagg ggtgaaagag gcgatgatgc agcacaggaa ggacgtgtta
      361 aagctggcag ggaatacggg catgatgtta ctggggatgg tcattttcct gtttaccgtg
      421 agtggcggga cgctctggta tcttggcggg atgatacagg cgaacctgga agaaatcagg
      481 aagcagaacg agagtctgga gaagctgaac gcgaagacat ggggcgtgga gtttgtgcag
      541 gacgggaaca ggaaatttct ggtccttcct tacgggaagt cagcagaggt gatttccttt
      601 caggggaagg aatgggtaca tctgaaggag tgacacagag tgacagagct ggaaacacat
      661 ttgctgaacg ccttagagca gctgcaacag gactatatgc agcggctgag cgaatgggag
      721 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
      781 aacgagcggg tcatgcagtt gagtcagcag gtgtagcact tgagcgagca gacagaacgc
      841 ttgagccagt tatacagcga gaactggaga taagagagga acggctgata caggagcgcg
      901 aacatgcgtt atccctggaa cgggagcgtc agccggaaat acaggaacgc acgctggatg
      961 gtccttcgct gggatggtga aaccatgaaa aatggcagct tcagtggatt aggaggggtt
     1021 aatgtggcct gtaccatctg gttgcatagg tattcatgcg gttaaaattt atcaggcgcg
     1081 atcgcggcag tttttcgggt ggtttgttgt catttttatc tgtctgctgc cgtgatcgca
     1141 ccaaacgcgt ttcagtggtg cgggcaataa gatattacgt taaattgtga gagaatggtc
     1201 cccatatact gactttatca tacggatagg gttggtagtg ggtcgattgg gtttcggcat
     1261 tgtttccgtt aatgccaatt gcatttttgt atttgatgca acagaaggcg tgagattctc
     1321 ctgagcctgg aaagtctaag tgtttagaat tgatggcaaa atatcttccg tccgattctt
     1381 gaagtagttg ttgtttggct gtctcccaaa gggtgctgtt acaaacttct tgaatttgta
     1441 gttgttgcag gactcttatt actgcgttgt catgttccaa ttctttaggg gagtgtatta
     1501 ttctctcttt taataagctg tttaccacag gatttagttg ttgtctaaag cacgcagata
     1561 atgccattgc tacacaattt tgttcccgag gaagcatgtt tggcggggta tgccgtgttt
     1621 gatgtgatat gtctattctc atagaaggtc tctggcttat agcatcaaca aatacggcta
     1681 taaaacaaaa ttttcataga gtgattcaag atcttcttga gatctttttt ggcggtgcgt
     1741 aatctgctgc ctgcaaacaa aaaaaccacc gttatcaacg gtggtttgtt tgccggaatc
     1801 tagagctacc aactctttct tccgaaggta actggcttgg tggagcacag ataccaaatg
     1861 ctgtccttct agtgtagccg cagttaggcc accacttcaa gaactcttaa tatctcaatc
     1921 caccttatcc agttaccaat ggatgctgcc agtggcgtta agtcgtgtct taccgggttg
     1981 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2041 acacagccca gcttggagcg aacgacctac accgaactga gataccaaca gcgtgagcta
     2101 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2161 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2221 cctgtcgggt ttcgccacct ctgacttgag cgtcg
//
LOCUS       DADLXX010000258         2136 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLXX010000258 DADLXX010000000
VERSION     DADLXX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.263"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..487
                     /locus_tag="JNX27_004135"
     CDS             14..487
                     /locus_tag="JNX27_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7885092.1"
                     /translation="MRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNX27_004136"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNX27_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7885093.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..2124
                     /gene="argF"
                     /locus_tag="JNX27_004137"
     CDS             1111..2124
                     /gene="argF"
                     /locus_tag="JNX27_004137"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7885094.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          544 a          520 c          532 g          540 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     2101 acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DADLXX010000259         2128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLXX010000259 DADLXX010000000
VERSION     DADLXX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.264"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(246..479)
                     /locus_tag="JNX27_004138"
                     /pseudo
     CDS             complement(246..479)
                     /locus_tag="JNX27_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..2098)
                     /locus_tag="JNX27_004139"
     CDS             complement(575..2098)
                     /locus_tag="JNX27_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7885095.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          636 a          467 c          451 g          574 t
ORIGIN      
        1 atgacattga tcactttggg gtgactattt tgtttaacaa ataactcttc gacatcaaaa
       61 ttattaatag ttgccatggt aattatttcg gtgatatata aaaacaactg tgataacacc
      121 aaggttgaca actatttgcg caataattcc tttccttcaa tgatccaggc gttcactgcc
      181 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      241 ggctgacgag tctttctctt aaatcgatta accagattga tcgccagcca aacaagggca
      301 ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg
      361 atgaccagga gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa
      421 tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat
      481 tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat
      541 aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc ttcaccttcg
      601 gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac
      661 cctgctgaaa tgtgatatta ttttggttca gatagccaag ttgttctttc gtttcgacac
      721 cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc
      781 gggttgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat
      841 caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg
      901 cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat
      961 tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt
     1021 cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat
     1081 taattccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc tgtctggcaa
     1141 cctgattcat caggctttgt gtaagcggaa cgattaatcc ggatttttca gcaagtggaa
     1201 taaatgccgc gggtgaaata tatccaccgt gaggttgttt ccagcgggct aacacctcaa
     1261 ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc
     1321 cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat
     1381 caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt
     1441 tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat
     1501 attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac
     1561 ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta
     1621 cattaagcgc cccgcgaata tgttgatcac ttatcgtaac caaaatgcgc gtatctgcaa
     1681 attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc ggagccaaca
     1741 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
     1801 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
     1861 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
     1921 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
     1981 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag
     2041 cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt
     2101 gcttgtcccg gccaaagggt ttaataca
//
LOCUS       DADLXX010000260         2118 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLXX010000260 DADLXX010000000
VERSION     DADLXX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.265"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            129..1166
                     /gene="curA"
                     /locus_tag="JNX27_004140"
     CDS             129..1166
                     /gene="curA"
                     /locus_tag="JNX27_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7885096.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1364..2029
                     /locus_tag="JNX27_004141"
     CDS             1364..2029
                     /locus_tag="JNX27_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7885097.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          583 a          454 c          567 g          514 t
ORIGIN      
        1 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
       61 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      121 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      181 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      241 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      301 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      361 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      421 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      481 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      541 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      601 cgacaggacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtga
      661 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      721 tttgccttga ttaccacgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      781 gtattgatat ctattatgaa aacgtgggcg gcaaggtatt tgatgcagtg ctaccattac
      841 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
      901 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
      961 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1021 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1081 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1141 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1201 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1261 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1321 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1381 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1441 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1501 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1561 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1621 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1681 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1741 cgacatggaa caaatcatta atgcgaacag gctatttcgc ttagcgattt atcatcgctc
     1801 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1861 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     1921 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     1981 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2041 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2101 taatttcgtc cgggcgca
//
LOCUS       DADLXX010000261         2088 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLXX010000261 DADLXX010000000
VERSION     DADLXX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.266"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(60..1025)
                     /locus_tag="JNX27_004142"
     CDS             complement(60..1025)
                     /locus_tag="JNX27_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAY7885098.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1878..>2088)
                     /locus_tag="JNX27_004143"
     CDS             complement(1878..>2088)
                     /locus_tag="JNX27_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885099.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          575 a          548 c          437 g          528 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gtgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac ccccaaaaca agcacccccg aacggcttac cccaaccttt
     1381 tccacgcacc cgatcaccaa aacagggaat aaaaacaaaa ccctgtctta caggtggatg
     1441 gcctcacttc gtgaaaccat ccaccccgca taagtttttt catcggtggt gtcgtcaatc
     1501 actaaatcgg aaccccaaag gggaccccca cgatttagca tttgacgaga ttaactctcg
     1561 gagatggcat catgctttta acggacttta aaaaacggtg aacggatgcg gattttaatc
     1621 cgtgtctgtg aggcgttgag caacgagcaa agcgaggcgg tagaaagaga gccagcggca
     1681 ggctcaggac gataatccgg ggaaaatgta ccactcgcag agtgttaaca tttgacatcg
     1741 gattgtctat cctgcattgg tgtcgagttg gcaaggctga ggataatgct gaggataatg
     1801 caggatagac agagcactag agattgcatg caatctcgta cctatcacct cagcctgccg
     1861 ctggctcttt tccagaacta agaatgcgtt cttaaaaatc caaacttgat accgtttaaa
     1921 tctattttca caaaaagagt ttttaccctc tcgtttgttt tctgaatata taaaattaat
     1981 tcatcctcaa aaccttcgtg cggatgaaaa ccaaatgtgc caaaacttaa tccatcaata
     2041 agcaaaataa ggtttttaaa cctgtcagga atcactgctt cacttctt
//
LOCUS       DADLXX010000262         2087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLXX010000262 DADLXX010000000
VERSION     DADLXX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.267"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..799
                     /locus_tag="JNX27_004144"
                     /pseudo
     CDS             1..799
                     /locus_tag="JNX27_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1238..2075
                     /locus_tag="JNX27_004145"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="JNX27_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          505 c          631 g          436 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc ggtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggt cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acttggtgat gaagtctctt gatcaggagc tctgatcaag agacagc
//
LOCUS       DADLXX010000263         2059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLXX010000263 DADLXX010000000
VERSION     DADLXX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.268"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNX27_004146"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNX27_004146"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY7885100.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          523 a          457 c          497 g          582 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg
     2041 ataaataggt ataaagaat
//
LOCUS       DADLXX010000264         2058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLXX010000264 DADLXX010000000
VERSION     DADLXX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.269"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(69..338)
                     /locus_tag="JNX27_004147"
     CDS             complement(69..338)
                     /locus_tag="JNX27_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7885101.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            395..1063
                     /locus_tag="JNX27_004148"
     CDS             395..1063
                     /locus_tag="JNX27_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7885102.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1379..>2058
                     /locus_tag="JNX27_004149"
     CDS             1379..>2058
                     /locus_tag="JNX27_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7885103.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          619 a          449 c          384 g          606 t
ORIGIN      
        1 tatcgaaagt ttaccagcca accgcagcac gtcctgcata cgtcgtgtct gcggtttttc
       61 tttttcgctt acattgtgtc tggttcttcc ggccactcaa tatcaggtgc agttgatgta
      121 tcaacacggt tcagcaacac ccgatacttc ttccaggctt ccagcaacga gttttcttcc
      181 tccgttgcga tctccagatc tacagcatcc tgaagtggcg caatatgctc actgaatccc
      241 tggatgtaga actgtgtggt gacggtcttc cagccattcg gctcctgctg tatcgaagca
      301 taccaggcta tttcaatatc gctatgctgc ggcagcattt aaccccttgt aattcatcgc
      361 cataattgat ttaattcaca aataaaacta taatatggtg aaattaatga aaaaaaacac
      421 agatgatggg gctaaaattt acacaccact taccctaaag ctttatgact ggtgggtttt
      481 gggagtatca aatcggcttg catggggatg tcctacaaag gaacaccttc ttccacactt
      541 tctggaacat ttaggtaaca accatctgga tattggtgtt ggaactgggt tttaccttac
      601 tcacgtacct gagagtagtc tgatatcttt aatggatttg aacgaagcta gcctgaacgc
      661 ggcatctaca agggctgggg aatcaaaaat taaacataaa attagccatg atgtttttga
      721 accttatcct gcggcgttac atggtcaatt tgattccatt tccatgtttt accttcttca
      781 ctgcctgcct ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt
      841 aactgatgat ggaactctat acggagccac aattcttggc gatggagttg tgcacaatag
      901 cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc atcttttcaa acacaaaaga
      961 ttccgaagaa ggcttaacac atatactctc agagcatttc gagaatgtta aaaccaaggt
     1021 tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg
     1081 ttcttgctta agacgtgcta cggtttcctt tacgatttgg ggttgcgact ttacctctat
     1141 tgataatgca ttccggcaga acgttcaaat atgaacgtac gatatttaac taaccgaaaa
     1201 acaaaatata gcacaggcga gacatttacc atctaagaaa atagtatcgt ttttttacag
     1261 ttaaatcaac atcacttcct taaaatgaaa acaataaatt tcaatcctga aacattttat
     1321 ttcagcttat tattccccag tatcacattt tatcaggata tccttctgcg aggttattat
     1381 gcttcctgta aataatcccc ccctatccac tggaaacgtc tctttttaca gaactacatc
     1441 aatcgacaat gttcacaata attatctctc cgaatgggtt gaatggacta aaaacagcat
     1501 ttccggagaa aacagggaaa ctgcttttac ccggctccaa ttatgtctgg agaacagtga
     1561 aacatcgttg gacttatctt gtttaggtct cagatctcta ccacgattgc ctgacaatct
     1621 tgatgaaatt aatgtaagca ataaccaact atcaatgctc cccgagctac caagggcatt
     1681 gaaagagctg aatgcaagca gtaatcaatt atctgcactt cctgaattac cagtgtcgct
     1741 ggaatatata aatgtgagtg ataaccattt gttcgcactt cctgaattac ctgcgtcact
     1801 agaatatatt aatgtaagtg acaatcacct gtctgtactt ccgaggttac caatgtcatt
     1861 ggaattactt gatgcagcca gaaatgcttt ggaagtaata ccagagtttc cagaaagaga
     1921 tgatcatatt ataagaatat tctggcttaa tcagaaccgg atcacggcaa ttccggaaag
     1981 catacttggc ctcagttctg atagcgttgt caatcttaga gaaaatcaac tatctcccag
     2041 aataatgcaa actttgtt
//
LOCUS       DADLXX010000265         2034 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLXX010000265 DADLXX010000000
VERSION     DADLXX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.270"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..362)
                     /locus_tag="JNX27_004150"
     CDS             complement(<1..362)
                     /locus_tag="JNX27_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7885104.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQPVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JNX27_004151"
     CDS             complement(429..1643)
                     /locus_tag="JNX27_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY7885105.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVMMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          492 c          414 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactggc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcat gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgactactac catacaagag agaaaaagcg attattcaac cgtgttcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aatg
//
LOCUS       DADLXX010000266         2031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLXX010000266 DADLXX010000000
VERSION     DADLXX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.271"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(210..374)
                     /locus_tag="JNX27_004152"
     CDS             complement(210..374)
                     /locus_tag="JNX27_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885106.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1275..1559
                     /locus_tag="JNX27_004153"
     CDS             1275..1559
                     /locus_tag="JNX27_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7885107.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1547..>2031
                     /locus_tag="JNX27_004154"
     CDS             1547..>2031
                     /locus_tag="JNX27_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7885108.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          523 a          426 c          582 g          500 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaaggaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg aaaatgtgct
      481 atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg gcatccggca
      541 tataggcagc atgaacgcaa cacaagctct cgtacggaac gtgggaacct gtcgtctcga
      601 tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga tgcgagacac
      661 aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga gcgaaggggg
      721 tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc atgagtcagc
      781 caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg gtcaaagcta
      841 atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt accaatcgaa
      901 gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg cccgcaaaca
      961 caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa ttcgtcacga
     1021 ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg ttcaacgaca
     1081 agagccgtat gacgcgagag tgtcacgtac ggttccgtgg gagcgtaggg gggcggtgcc
     1141 cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa atgccgctga
     1201 cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt caatatgcta
     1261 ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag ctcaatctca
     1321 gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt cataagtcgc
     1381 gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt ctcgaccggc
     1441 gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt gatgctccgg
     1501 ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg gatgtgtaac
     1561 tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagcg gtgaagctgt
     1621 acttgatgac tggttaaagc agaaggggct aaaaaatcag gcaatcggag ccacccggac
     1681 ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac tggccactgg
     1741 tagtatcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag accccattcc
     1801 tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag ggcttggcgc
     1861 ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata tcggtgtgcg
     1921 tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc atcatggttt
     1981 taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat a
//
LOCUS       DADLXX010000267         2028 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLXX010000267 DADLXX010000000
VERSION     DADLXX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.272"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1155)
                     /locus_tag="JNX27_004155"
                     /pseudo
     CDS             complement(1..1155)
                     /locus_tag="JNX27_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
     gene            1237..1464
                     /gene="vapB"
                     /locus_tag="JNX27_004156"
     CDS             1237..1464
                     /gene="vapB"
                     /locus_tag="JNX27_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7885109.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1464..1862
                     /gene="vapC"
                     /locus_tag="JNX27_004157"
     CDS             1464..1862
                     /gene="vapC"
                     /locus_tag="JNX27_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7885110.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(1871..>2028)
                     /locus_tag="JNX27_004158"
     CDS             complement(1871..>2028)
                     /locus_tag="JNX27_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY7885111.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
BASE COUNT          452 a          603 c          502 g          471 t
ORIGIN      
        1 ccggacgtga acagcccctt ctcacgatcg agggggataa gctgctcacg gctgatggcc
       61 tcatcgatac cggcgcgtgc ccgttcaatc acaccatttt ccggcggcag tatgccgacc
      121 gtcctggcca gcaggtccgt atacataaac tgcactttgc gttcacttaa tccggcaatg
      181 gcctgtgtca ccgcctgctg cacatccggc ccgtcctgtg aagcgggtcc gggtgtctgc
      241 gtgcgggtat atgcccgttg ttccgctgcg tcacgatatg cccggatgtc gaacccggtt
      301 tccttcagcg tctgcatcca ctcggccatt ctgacttcag gatcgacgtg ctgtttggat
      361 ttacgcgtat ccagcgccgc cacatcccgg gatttcagcg aggcgtcttc cccgacggcc
      421 tcccggatag tctgtgagcg tccggaaaag gcctccaccg gtacacccgg catttcccac
      481 ataccgtgct taccgaccac ttcagtttca tagcccagcg cctcaacctg ctctttcagt
      541 ttttcccggt agagcctgcc aaaggcaatc tgattagcgt acacattctc aatgaacccc
      601 gttttcccca ctttgtcact gctcagtgtc ttccattcgc cgttatactg cgtaacatta
      661 gtcaccaccg catgcgtgtg taactgtggt tcctgatcgc gactggtgtc gtggttaaac
      721 agtgccatca ccagattacc ggtcagcacc gtttctgact gtccgtccgt catcacccgt
      781 gtggaggcca gtgcctccac acgggtgatg acggacagca aaatccacgg cctggttatg
      841 tgcttcaata agacgtttgt cccccccgag catggccatc atggagatac ttttgggagc
      901 ggagaaggtc aggtcgtagc cgggacgatg cctgttactg ccatcctgca tgcggcttaa
      961 atccgccccg tccggcagcc tgccctccag aaggcgggta aagacatcct tatcgacact
     1021 gccctgcagc cccagttgtt cagccccctg gccggcccag cgttctccca tgctgcccag
     1081 cacatagtaa ttatccttgt cggtataata attaccggca cttccggccg atctgacctg
     1141 cgcgatactc atcataaacg tatatccctt tgacatatcc cggtatcaat cccacaatag
     1201 atatacacaa gacatatcca cataaggagg caaataatgg aaaccaccgt atttctcagc
     1261 aaccgcagcc aggcggtcag actgccaaaa gcggttgcat tgccggaaaa cgtaaaacgc
     1321 gttgaagtga ttgctgtcgg acgaaccaga atcattacgc cagccggaga gacatgggac
     1381 gaatggttcg acggacacag cgtcagcacc gattttatgg ataacaggga acagcccggc
     1441 atgcaggaaa gggagtcatt ctgatgctga agtttatgct cgataccaac atctgcattt
     1501 ttacgataaa gaacaaaccc gccagcgtca gggagcgttt taacctgaac caggggaaaa
     1561 tgtgtatcag ttcggtcacc ctgatggagc tgatatatgg tgcagaaaaa agccagatgc
     1621 ctgaacgtaa tctcgctgtg atcgagggat ttgtttcccg cattgatgtt ctggattacg
     1681 acgctgctgc agccacacac accggccaga taagagcaga acttgcccgt cagggacgcc
     1741 ctgtcgggcc atttgatcaa atgatcgcag gtcatgcccg cagtcggggg ctgattattg
     1801 tgactaataa cacccgggaa tttgaacgtg tgggcggcct gagaactgaa gactggagct
     1861 gacctgttca tcagaaatca tctcccggct caacatcctc cccgcgttcc cggtgcacat
     1921 tgatgttcac ctcttcacga cgctgcatct gctgccaggt gtccggatga ttttcctgct
     1981 gccatgcctc ataagcggcc atatccacca cttcaccgga ttcactga
//
LOCUS       DADLXX010000268         2027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLXX010000268 DADLXX010000000
VERSION     DADLXX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.273"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            396..649
                     /locus_tag="JNX27_004159"
                     /pseudo
     CDS             396..649
                     /locus_tag="JNX27_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            806..>2027
                     /locus_tag="JNX27_004160"
     CDS             806..>2027
                     /locus_tag="JNX27_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7885112.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAA"
BASE COUNT          535 a          407 c          458 g          627 t
ORIGIN      
        1 caatttcgca cgtataaaca tctttgacgc cagcgagata cagccatcct tcccgggtgg
       61 caacatacgt tatatctgcg acccagacct gattaggggg gttggggagc aatggaacag
      121 taaacgccgt taagaagtca atttaaatag ccaaaaatta actagccaat ttacattgca
      181 ggttgtaatt tttaaccacc ttttttacga tagccaaatt acctataccc atcccggccc
      241 tgccaagcta tacccgctat cggcgatgaa aatgcttttg tgcgcggtcg gagtggtccc
      301 gacgagggtt ttacccgaag tcggggcgta tctccgcgtt agcgggccgt gagggccgct
      361 tacgagcgtg tatgagagac gtacagtggt tttgtccgct cacaccagta catacaggta
      421 cggaaataaa accagtcgaa atgccggtaa cgacgattac tcctgtctct gatgccggtg
      481 gactacaaga ttttatttac tggcgtcctg atgcggacag gactggtatg gaacctgttt
      541 atgtgatact taatgatcct ctggattccg ggcgatttac gcgtaaacaa ctaaataaaa
      601 atatcttaaa catgcaagta attttggtat tctcgataca agaataaata gtagtaacta
      661 ctatagttgc aaataatgtt aaaaacatct ctatgatcat ctttacattt ttcatgattg
      721 acaattgttt ttggttaaaa aaagacaact aatgagtgtg ataatgcaat actttgagtg
      781 attatataat gataagtaaa caggtttgcg acaattggtt atcagtatta cagaaggatt
      841 aaatatgtca ttattcacag aacctaagga aattgaaaga ttaccctctg aagagattga
      901 acgattatat cccgtcctgc gatacagggt ctttatcagc attttcctcg gatatatggg
      961 gtattatttt gtccgaaaca ccaccagtgt gttatccggt gtgctgcaca tgtctgcaac
     1021 cgagatcggc attatctcct gtgccggttt tttatcctat ggtatatcaa agtttgtttc
     1081 cggacttatt tctgacagaa gtaattcaaa agtatttttg tctcttggtt tattcctgtc
     1141 cggactggta aattttctga taggatatat tcccgggatc atcacatccg ttacgttatt
     1201 ttcaacaatg tatttactta acggctggat ccaaggtatg ggatatcctc ctggtgccaa
     1261 aacactggtg ttctggtatg aacatcggga aaggatcacc tgggccacac tgtggaattt
     1321 atcgcacaat gtcgggggag ctctggcccc cgttctgatt ggtttcagtt ttggtttttt
     1381 cggcgacagt gctctggagc atgcccgggc cgcatttatc ttcccaggag tactgtgtat
     1441 ggcaatgagt gtgctgattt actttattca ggtggacaga cctgtatcag tggggcttcc
     1501 tcccattgaa gaatggaaag gtaacgtcgt ttcacacccg gctaaaggcc gtgaacaagg
     1561 tccgagactc tccattcctg atatcatcag aaaacatatc atccgaaata ataaactcat
     1621 ttactgctgt atctatggtt cgtttgttta tattctgaga tacggtattg tttcctgggc
     1681 tcctaagttt ttaagtgaca gtctggatgt cggtggtaag gatatgggaa aactggcatc
     1741 aatggggggg ttttctgttt ttgaaatcgg tggtgtggct ggtatgcttc ttgccggata
     1801 cctgtctgtc agactgttca gaaactcaaa accattaaca aatactttat ttctggcact
     1861 taccattatt ttattaatcg cgtactggta cgtgccatca ggcaatgaat atctctggct
     1921 aaactataca atccttattc tgttggggct ggcagtgtat ggtccggtga tgtttattgg
     1981 tctttattca atggagcttg ttcctaaaga agctgccgga gccgcat
//
LOCUS       DADLXX010000269         1978 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLXX010000269 DADLXX010000000
VERSION     DADLXX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.274"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNX27_004161"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNX27_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7885113.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1978
                     /locus_tag="JNX27_004162"
     CDS             1494..>1978
                     /locus_tag="JNX27_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7885114.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     V"
BASE COUNT          563 a          341 c          477 g          597 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtctt
//
LOCUS       DADLXX010000270         1889 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLXX010000270 DADLXX010000000
VERSION     DADLXX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.275"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..66)
                     /locus_tag="JNX27_004163"
     CDS             complement(<1..66)
                     /locus_tag="JNX27_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885115.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
     gene            complement(63..419)
                     /locus_tag="JNX27_004164"
                     /pseudo
     CDS             complement(63..419)
                     /locus_tag="JNX27_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            499..1692
                     /locus_tag="JNX27_004165"
     CDS             499..1692
                     /locus_tag="JNX27_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY7885116.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1718..1889)
                     /locus_tag="JNX27_004166"
                     /pseudo
     CDS             complement(1718..1889)
                     /locus_tag="JNX27_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          514 a          433 c          466 g          476 t
ORIGIN      
        1 aaggctttcc agttgcaact gcccggcgag cgccatcagt cgttgatgtt gcagttccat
       61 catcacgcca ctcctctgca gaatgagtcg tagatggaga gtggatgatg ctgggggggg
      121 ttgtcgaagc tcaccagatt ttcaccagga tgcacgtcat actctttttt ctccggaggc
      181 agtgccagca tggactgctg ctcttcgagc cagcgatcac agggacgggc ctggattgtt
      241 tcatgctttc gttggttagc gacatcgtgc agccagcgca gaccgtggcg gctggctgtt
      301 tcaacatcga cagtgctccc catcgggcgc aggcgagtca ttagtgggat gtaaaaactg
      361 ttacgggtgt actgcaccat ccgttccacc ttacctttag tctgtgccct gaagggctga
      421 ggaatcccca gaaaacgaga caaacatagc attatgtgat ctactgattt gtgccaacaa
      481 ttcaatgaga tcactgctat gcctgttcat aaagtatgtc agaatttctt ccgggatgcc
      541 ttagcaccat ttcatcagta ccgacaaaat gccctgatgg atgccacaat ggcgttaatc
      601 aacggagcat ctctgacgca gaccagtatc ggacgttttt tgccgggtaa tgcacaagtt
      661 aagaataaga tcaaacgtat tgatcgcctc atgggtaatg aggcgctcca tcgcgatatt
      721 ccaatgattt tcaggaatat cacttcaatg ctgacacggc agttatcttt atgtgtaata
      781 gccgtagact ggagtggcta tccctcccag gagcatcatg ttcttcgtgc cagccttctg
      841 tgtgacgggc gttccattcc gttattgagt aaggttgttc cttctgaaaa acaaaataat
      901 ccattgatac aacatgattt ccttgattcc cttgctcaat ccctgccccc cgatgccagg
      961 gttattattg tcacggatgc tggatttcag agcgcatggt ttcaccatat tacttctctg
     1021 ggctgggatt ttattggtcg gatcagaaat aacgttcagt attgcctcga taacgcgcct
     1081 gaaagatggt taaaagtgtc ggatagccct gaatgtaaaa caccggaata tatgggggct
     1141 ggaaggctgg ttaaagaaag gaaaaaaagc atccgggggc atttttacac ctataagaaa
     1201 tcggcaaaag ggcgaaaaaa gaaacgttcg aaaggtcaga gtggtttaaa caaaacagac
     1261 aaagaacaga gcaaatcagc aaaagaagcc tggttgatct tcagcagtac aaatgacttc
     1321 agagcgcgag aaatcatcaa gttatacagt cggcgaatgc agatagagca aaactttcgt
     1381 gatgaaaaga acgggcgctt cggatttggt cttcgggcca gcaaaagtcg ttcaacagga
     1441 agaattctgg ttctgagtct gctggcaacg ttgagcacta tcgtaatgtg gttactcggt
     1501 tatcacgctg aaaataaagg gttacacctg aaatatcagg ctaacagcat taaatcccgg
     1561 cgggttatct catatctgac gttagcgaag aatgttcttc gacactcgcc acttatttta
     1621 agacgaacag tactgagcac agttctaaac catctgtcca gaacctaccg aaatatggtg
     1681 ctggtttatt agcagtaatt ttgtggggat ccctcagcct gaaggggcga cacagtcggg
     1741 gagagaagcc catctccttg ccgaactgcc acagcgaagg atggaaccgg tgctgaccgg
     1801 tctgatatgc gtcacgttgc agaaccacag ttttcatatt gtcatacaac acttcgcgcg
     1861 gcacaccacc aaagaagcgg aacgcatta
//
LOCUS       DADLXX010000271         1869 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLXX010000271 DADLXX010000000
VERSION     DADLXX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.276"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..1869
                     /gene="cyoB"
                     /locus_tag="JNX27_004167"
                     /pseudo
     CDS             27..1869
                     /gene="cyoB"
                     /locus_tag="JNX27_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          356 a          487 c          504 g          522 t
ORIGIN      
        1 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
       61 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
      121 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
      181 tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
      241 gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggtgaagcgg
      301 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct
      361 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
      421 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg
      481 gcgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg
      541 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat tactggatat
      601 ggcgtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
      661 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctaggcat
      721 cactgtgcac gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
      781 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
      841 tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc
      901 tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
      961 gttatacctc gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc atcgtttggc
     1021 tgcaccactt ctttacgatg ggggcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     1081 caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc
     1141 agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc gtcaccttct
     1201 cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     1261 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     1321 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     1381 ggggtaaacg cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccgctgt
     1441 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     1501 acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt attctctgcc
     1561 tcgttattca gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg
     1621 acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact
     1681 ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg
     1741 aagcgtataa aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag
     1801 gtatcgtcat tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt
     1861 ggctggcga
//
LOCUS       DADLXX010000272         1839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLXX010000272 DADLXX010000000
VERSION     DADLXX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.277"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>1839
                     /locus_tag="JNX27_004168"
     CDS             <1..>1839
                     /locus_tag="JNX27_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7885117.1"
                     /translation="MAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
                     TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESV
                     AQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVM
                     EQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVAD
                     GERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLEN
                     GWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARH
                     PSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLL
                     TEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRH
                     ILEGKEAVTPLMERVPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRA
                     VMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDF
                     SNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPG"
BASE COUNT          391 a          503 c          614 g          331 t
ORIGIN      
        1 catggccttc acgccgggca gtaccgttat cgtcgaccag ggtgaaaaac tctccctgaa
       61 agagacgtta accctgctgg atggtgccgc acgtcataac gtacaggtcc tgataaccga
      121 cagcgggcag cgaaccggta caggcagtgc gctgatggcc atgaaggatg ccggggtgaa
      181 cacatatcgc tggcagggcg gagaacagcg accggccacc atcatcagtg aaccggaccg
      241 taatgtccgc tatgcccggc tggccggaga ttttgcggcc agcgtgaaag ccggagaaga
      301 gagcgtggca caggtcagcg gggtacggga acaggccata ctgacacagg ccattcgcag
      361 tgagctgaaa acgcagggcg tgctcggaca ccctgaggtg accatgactg cactgtcacc
      421 ggtctggctg gacagccgga gccgttatct gcgggatatg taccgaccgg ggatggtgat
      481 ggagcagtgg aacccggaga cacgcagtca tgaccgctat gttatcgacc gggtgacggc
      541 gcagagtcac agcctgaccc tgcgggatgc gcagggcgaa acgcaggtgg tgcgtatttc
      601 ctccctggac agcagctggt cgctgttccg gccggaaaaa atgccggtgg cagacggcga
      661 gcgactgagg gtgacaggga aaattcccgg actccgcgtc tccggcggtg accgcctgca
      721 ggtggcatcc gtcagtgaag atgcgatgac ggttgttgtg ccggggcggg ctgaaccggc
      781 caccctgcct gtggctgatt caccgttcac ggcactgaag ctggagaacg gctgggtgga
      841 aacgcccggg cattccgtca gtgacagtgc gacggtgttt gcctccgtca cacagatggc
      901 aatggacaac gccaccctga acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc
      961 ctcactggat gaaacccgta ctgcggaaaa actcgcccgc catccgtcct ttacggtggt
     1021 ttctgagcag ataaaggcgc gtgccggtga gacattgctg gaaaccgcta tcagtctgca
     1081 gaaagccggg cttcacacgc cggcacagca ggccattcat ctggcccttc ctgtgctgga
     1141 aagtaaaaac ctggccttca gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc
     1201 agaaggaacc ggttttactg aactgggagg ggaaatcaat gcgcagataa aacgcggtga
     1261 cttactgtat gtggatgtgg caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc
     1321 gtatgaggca gaaaagagca ttcttcgcca tattctcgaa ggcaaggagg cggtcacgcc
     1381 gctgatggag agagtacccg gcgaactcat ggagacgtta acgtcgggac agcgtgccgc
     1441 cacccgtatg atactggaaa cgtccgaccg tttcacggtg gtacaggggt atgccggtgt
     1501 gggtaagacc actcagttcc gggcggtgat gtcagccgtg aacatgctgc cggagagtga
     1561 gcgtccccgg gttgtggggc tggggcccac gcaccgtgcg gtcggtgaga tgcgcagcgc
     1621 cggcgtggat gcacagacac tggcgtcctt tctgcatgac acgcagctgc agcagcgcag
     1681 cggagaaacg ccggatttca gcaacacgct gtttctgctc gatgagagct caatggtggg
     1741 caataccgac atggcacggg catacgccct gattgcggcc ggtggcggtc gtgctgtggc
     1801 cagcggtgac acggaccagc tgcaggccat cgcgcccgg
//
LOCUS       DADLXX010000273         1828 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLXX010000273 DADLXX010000000
VERSION     DADLXX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.278"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..168
                     /locus_tag="JNX27_004169"
     CDS             <1..168
                     /locus_tag="JNX27_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7885118.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="JNX27_004170"
     CDS             153..791
                     /locus_tag="JNX27_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7885119.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1410
                     /locus_tag="JNX27_004171"
                     /pseudo
     CDS             1019..1410
                     /locus_tag="JNX27_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1429..1800
                     /locus_tag="JNX27_004172"
                     /pseudo
     CDS             1429..1800
                     /locus_tag="JNX27_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          544 a          324 c          435 g          525 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagtgggggg ggagatgaac cctatgcaat gtcctggtta
     1441 atatctcagt gtgctcatca gtgcactgat aataaaaaga cggagacaga tgccatttac
     1501 gataaagttc ggagttctta tttgctttca tgtattctga aaaaaaacaa aaatgttggg
     1561 cttattcttc atgcaccatc atttgtttct gtgtctgaaa aaatcgccag aattgtgatg
     1621 gcaaattact cccgtaactg gagtaattcc gagttggcca gtgcggtttt aatgagtgaa
     1681 tcttctttaa aaagaagaat gtacaaagag gtcggttcta taagtacatt tgtacataaa
     1741 ataaagctta cagaagctat caggaaatta cgaaggacaa atactcccat ttcggtaatg
     1801 ctgccaactt actgatttag tgtatgat
//
LOCUS       DADLXX010000274         1826 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLXX010000274 DADLXX010000000
VERSION     DADLXX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.279"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(39..308)
                     /locus_tag="JNX27_004173"
                     /pseudo
     CDS             complement(39..308)
                     /locus_tag="JNX27_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(423..1061)
                     /locus_tag="JNX27_004174"
     CDS             complement(423..1061)
                     /locus_tag="JNX27_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885120.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1220..1690)
                     /locus_tag="JNX27_004175"
                     /pseudo
     CDS             complement(1220..1690)
                     /locus_tag="JNX27_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          515 a          452 c          337 g          522 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggagccagtc tacccagcaa gcgctgtaac
       61 tcttttattt gttttatagc ggcagccagt tctgaggcag gaacgacatc ctctccggca
      121 gcaacagcag tgagacttcc ttccagatat tattttttcc acttaagcaa caggctgggc
      181 tgaataccat gcaaacgcgc cacatgggac accgtcatgc caggctccat tgtctgctga
      241 ataatggcaa ttttttcctg aggagtttta cgtttacgaa gctccagccc cagcaaagtt
      301 ccagtcatct caaaattaga actagtgtta gacatatatc caagcctatc tcttatttta
      361 agatacatcc actgtctggc gtttcaaggg gccggctcaa atctatgtac aggctctcag
      421 catcagcaaa gcctcttact tttccaacta ctactgttag catagtgatc aaattcctga
      481 tcagatacat gaattacctg aaccgaacta ttttcatcta caaatgcatc tggcgatttt
      541 tctacaggat cataaagatt aaatcccttt cctgtttgaa catccaaaaa cataattttt
      601 tcaccatcgt taaatccagc tatccaatga gtggcctgtt caatagaaat cattactaca
      661 cttccttttg aagtattttg taaaatattt tctgcaatac gctcacgaaa tttactataa
      721 gtttcattag gatgtgacac tatgccttca ctatcaattt gctctaaatg cctatgaaaa
      781 aatccggtca tatactctga tatttcatta ggtacgggag cctctcttac tccataccct
      841 aacatagcca tacaagcatg aagcgcacac cctgaacagt taccatcccc accagacgga
      901 tttatcattt tctgtaaagt cgctgccaat tcagaatctt tatccatatc accaagactt
      961 ttagagttta aagattccag caatttttta acatttacat ttccctcctc tgtatgtgcc
     1021 tcatatccag cagttccctg taacgacacc ccattaatca tattgtatcc tgacagtata
     1081 tatactcact atattttctt cataacaaat atccctctga tatgacaaca aaaaacggag
     1141 ccggattcct gtttttgtga actgcatcta ttcgtgtcta aaaagtgatc cacttaccac
     1201 caacggccta atatatacag aacggagtaa acagggctaa cagttcccga caggtctcat
     1261 cattccgtgg cccgaaagtg taagccaaca caccgccagt tttagtgttg tacgcatacc
     1321 agagccagtg ttgttgcagc cttgctgcca acgaagcgcc actgctcatc aagttcacag
     1381 ataagggcaa catcagcgtg agcgactggc gcagatgtta tccgccgggg cgggagcttt
     1441 ttaatgtccg tatgacggta ttgatgccga ttttcagtgt cctggcggta tcacggcccc
     1501 ctgcgccgtt atgtgccatt tcaacgatct gctccttaac gccgggtttt ctggcttcat
     1561 aagagtaagt gagctggaaa acacgtttac atgagcgaca acggaaacgc tcatgccggg
     1621 aacaactacg gccatgtcga taaacttcat cggaatgaca gcgcggacaa tgaacagtaa
     1681 cggtagccat ggaaaacctt aaataccagt agattaccag aaacttctgt cgattgaaga
     1741 catcacccga agtttgaatt cattcgaata ctttttacga ggttcttttc gccagtcctg
     1801 taattcgtaa gcgccccatc agcgac
//
LOCUS       DADLXX010000275         1820 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLXX010000275 DADLXX010000000
VERSION     DADLXX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.280"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..185)
                     /locus_tag="JNX27_004176"
     CDS             complement(<1..185)
                     /locus_tag="JNX27_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885121.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVKQLRDERQG"
     gene            371..1213
                     /locus_tag="JNX27_004177"
     CDS             371..1213
                     /locus_tag="JNX27_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7885122.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1216..>1820
                     /gene="capU"
                     /locus_tag="JNX27_004178"
     CDS             1216..>1820
                     /gene="capU"
                     /locus_tag="JNX27_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7885123.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
BASE COUNT          528 a          338 c          397 g          557 t
ORIGIN      
        1 ccctgccgct catcacgtaa ttgtttcacc catcgcgtca ttgtggaaag gccgacatcc
       61 atagcgctgg ctgcatctgc cacggtgtat ttctggtcaa cgaccagttg agcggattcg
      121 cgtttaaact ctgcgctgaa atttcttttt tcattatggc acctgtgttg ttctgaggtg
      181 agcatatcac ctctgttcag gtagctaaat ttagtgtgcc acctcaaggt tcttagtgag
      241 ccgtttttaa aagatatgat aatatattat tgtttataaa ttgttgatgt ttgatttagt
      301 agtatataaa gttcaccacc tttattaggc tttcctttta tctctactta ctgggtgcca
      361 gaaactttaa attttaaacg gagggggcat tttgtttaaa gcaaatcatg ttccggtatt
      421 aatgtatcat catgtcagcc attgcccagg actggttaca ctttctcccg agacattccg
      481 gaaacaaatg aaatggttgg ctgaaaacaa ctggaaaaca ctgtcatcag atgaactgga
      541 gtttttttat cggggcggaa aacttccccg taaaagcgtt atgttgacat ttgatgatgg
      601 ataccttgac aactggtttc aggtatatcc tttattaaac gagtttaatc tcaaggcaca
      661 tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc ctggaaagga
      721 atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata atgttatgct
      781 tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc atgttcatac
      841 acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt gtaaacatct
      901 tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa aatgcagtaa
      961 acatttgtgt tggccggaag gttattataa caaggattat attcagatcg ctgaagaact
     1021 tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta aaggaactac
     1081 acggatagga agaataagca ccaaggaacg ggaaaattgc gtatggctga aaagacgact
     1141 tttttattat acaacaccgt ttttttcatc gttgctggcc cttcataagg ggccaagatt
     1201 accggatgac taataatgaa tatactattt acggaatcat caccgaatat aggtggtcag
     1261 gaattacagg ctgttgctca aatgaaggcc ctgaagaaaa tggggcattc agttctgctt
     1321 gtctgcaggg agaacagcaa aattgctttt gaagccagta agtttggaat tgatatcaca
     1381 ttcgcgttat ttcgaaacag tcttcacatt cctactgtat ggagattact cggaatagtt
     1441 catagttttc agcccgatgc aatcgtttgt cacagtggac atgatagtaa tattgttggt
     1501 ttggtacggt tttttacctg gaaacatcca ttcagaatta tcaggcaaaa gacatatttg
     1561 acacgaaaaa caaaattttt ttcaataaat catttttgcg atgaggtgat tgttcccgga
     1621 acaaatatga agacacatct ggagcaggaa ggatgtcgaa ctcgggttac tgttgtgcct
     1681 cctggctttg atttccagga attatacgtt gattcccgaa acagtttgcc tccgagtgtt
     1741 ctttcctggc tggcgtcccg aagaggatgc cccgtaattg ctcaggtagg aatgttgcgc
     1801 ccggaaaaag ggcacgaatt
//
LOCUS       DADLXX010000276         1812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLXX010000276 DADLXX010000000
VERSION     DADLXX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.282"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..1147
                     /locus_tag="JNX27_004179"
                     /pseudo
     CDS             1..1147
                     /locus_tag="JNX27_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1286..1798
                     /locus_tag="JNX27_004180"
     CDS             1286..1798
                     /locus_tag="JNX27_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885124.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
BASE COUNT          555 a          341 c          452 g          464 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag actgagttat agtacggtat ggagaggcat
      241 agcattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctagccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag agcggggata aaggattagt
      901 aaccttaaaa cagaaagagg gtaaacccgt ccccttcggc gctgtgatta gttatagcaa
      961 agacactgaa aatatggccg gcattgtagg tgaagatggt attgcttatg tttctggctt
     1021 gagtgcggaa ggcgagttta atgtgaaatg gggttattct aaagatcaat cctgtatcgc
     1081 aaaataccaa cttcctgcga agaaaagtgc atcagggtta taccagatag cggcgacatg
     1141 tttatagttt ctaaattaag aaactcataa tttatttttg tgaagatgtt tccattattt
     1201 ggtggaagca ttttcgcgat agcacgtaaa tgcaaagaaa gaaactgcat tttgcagtgt
     1261 aaatgaaaat catggagaaa atataatgaa gaaaacattg ttagcaatca tgctggcggg
     1321 gacagctttt gcttctcagg cgggaacttt agtaagccag ggtactgaag cgtcagctaa
     1381 tcttactttg accaagccta tcgttgttaa taataccatt caaccagtga aaggggtata
     1441 tagcggtacg ttaactgcat ggacgccttt ggcaacaggt attgttggcg catctgatgg
     1501 gcagagccac gactatgctg taactttccc ggatgatatt tatgcggaga gcagtacttc
     1561 agcggatgca gtaatttctg gtgacaataa cccggaccat aaactgaaag tttccctgac
     1621 aacgcttgag caggatcctc cttcagctgc ctccgaagag attggcggta agcgttatat
     1681 gatgctgaaa aatactggaa caggtggtgc ctatcgcgtc gtttcccata tgaaagaaca
     1741 ggttgttgag ccagactctt acaccatccg tacccaggct tatatctacg cagaataatg
     1801 cagatgatta gt
//
LOCUS       DADLXX010000277         1782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLXX010000277 DADLXX010000000
VERSION     DADLXX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.283"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..452
                     /locus_tag="JNX27_004181"
     CDS             <1..452
                     /locus_tag="JNX27_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7885125.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..988
                     /locus_tag="JNX27_004182"
     CDS             455..988
                     /locus_tag="JNX27_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7885126.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="JNX27_004183"
     CDS             complement(1017..1544)
                     /locus_tag="JNX27_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7885127.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="JNX27_004184"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="JNX27_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435977.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          499 a          391 c          399 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcaaag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DADLXX010000278         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLXX010000278 DADLXX010000000
VERSION     DADLXX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.284"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..284
                     /locus_tag="JNX27_004185"
     CDS             <1..284
                     /locus_tag="JNX27_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885128.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="JNX27_004186"
                     /pseudo
     CDS             286..890
                     /locus_tag="JNX27_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1773
                     /locus_tag="JNX27_004187"
     CDS             877..>1773
                     /locus_tag="JNX27_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7885129.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLPDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          426 a          403 c          496 g          448 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 ccagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttc
//
LOCUS       DADLXX010000279         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLXX010000279 DADLXX010000000
VERSION     DADLXX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.285"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..219)
                     /locus_tag="JNX27_004188"
     CDS             complement(<1..219)
                     /locus_tag="JNX27_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7885130.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..1307
                     /locus_tag="JNX27_004189"
     CDS             525..1307
                     /locus_tag="JNX27_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY7885131.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(1309..1407)
                     /locus_tag="JNX27_004190"
     CDS             complement(1309..1407)
                     /locus_tag="JNX27_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY7885132.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT          499 a          421 c          355 g          498 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gcatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa acgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcgttg aatgaaaagc ctaatgtaac agcaatactt tgtggtggta
      961 cgtatcgtgc cagaagtgat gctttttacg atgccagtaa ctcttcgcca ttagactctc
     1021 tcaatccgcg aaaaatattt atttccgcca gcggtgtgca taatcacttt ggcgtcagct
     1081 ggtttaaccc tgaagatctt gccactaagc gtaaagcgat gaaccgtgga ctacggaaaa
     1141 ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc tgccagcctc gcaccgatct
     1201 ctgcatttga cgttctgatt agcgatcgtc cgttaccggc agattatgtt acgcactgcc
     1261 agaatggttc tgtaaagatc attacacctg attcagaaga cgaatgactt actgaaaaaa
     1321 caccacaatc ttgttaaaca tcgtcgggtt ggactgatta cgttgcactt tcaccacata
     1381 ttccagctta tctatttggc ttatcaccta ctccagacgc tggtcatcct tgaccagtag
     1441 ccagatatgg cttttgtcgc agtcctgaat cggcaggcag agaatgtctt caacgttaag
     1501 agcgcgacgg gcaaaagccc acaaaggtga gtcattacac ccggatggta aacaggcact
     1561 tccttgttgt agaagccgtt atattgtcca taatgtctgg gtttttaatg catactgggt
     1621 aacaaccgga cgaatcttcg aatatcactg gcgctgccag ccggtttgaa ttcatctcac
     1681 aaccctgcat agagcgaatc tcctgcctgc acgtcactct gaatcgccac gggtttaaca
     1741 gacacctcag agtcatttaa gatggcttaa aga
//
LOCUS       DADLXX010000280         1758 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLXX010000280 DADLXX010000000
VERSION     DADLXX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.286"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..1728
                     /locus_tag="JNX27_004191"
                     /pseudo
     CDS             1..1728
                     /locus_tag="JNX27_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          390 a          470 c          568 g          330 t
ORIGIN      
        1 gccttcacgc cgggcagtac cgttatcgtc gaccagggcg aaaaactctc cctgaaagag
       61 acgttaaccc tgctggatgg tgccgcacgt cataacgtac aggtcctgat aaccgacagc
      121 gggcagcgaa ccggtacagg cagtgcactg atggccatga aggatgccgg ggtgaacaca
      181 tatcgctggc aggggggaga acagcgaccg gccaccatca tcagtgaacc ggaccgtaat
      241 gtccgctatg cccggctggc cggagatttt gcggccagcg tgaaagccgg agaagagagc
      301 gtggcacagg tcagcggagt acgggaacag gccatactga cacaggccat tcgcagtgag
      361 ctgaaaacac agggcgtgct cggacacccg gaggtaacca tgaccgccct gtcaccggtc
      421 tggctggaca gccggagccg ttatctgcgg gatatgtacc gcccgggaat ggtgatggag
      481 ctgtggaacc cggagacacg cagtcatgac cgctatgtga cagaacgtgt gacggcgcag
      541 agtcacagcc tgaccctgcg gaatgcgcag ggcgaaacgc aagtggtgcg tatttcttcc
      601 ctggacagca gctggtcgct gttccggccg gaaaaaatgc cggtggcaga cggtgagcgt
      661 ctgagggtga cagggaaaat tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg
      721 gcatccgtca gtgaagatgc gatgacggtt gttgtgccgg gacgggctga accggccacc
      781 ctgcctgtga gcgattcacc gttcacggca ctgaagctgg agaacggctg ggtggaaacg
      841 cccgggcatt ccgtcagcga cagtgcgacg gtttttgcct ccgtcacaca gatggcaatg
      901 gacaatgcca ccctgaatgg tctggcccgc agtggtcgtg atgtccggct gtattcctca
      961 ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc cctcctttac ggtggtttct
     1021 gagcagataa aggcgcgtgc cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa
     1081 gccgggcttc acacgccggc acagcaggcc attcatctgg cccttcctgt gctggaaagt
     1141 aaaaacctgg ccttcagcat ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa
     1201 ggaaccggtt ttgctgacct gggaggggaa atcaatgcgc agataaaacg gggtgattta
     1261 ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc tggtttcccg tgcgtcgtat
     1321 gaggcagaaa agagcattct tcgccatatt ctcgaaggta aggaggcggt cacgccgctg
     1381 atggagagag tacctggcga actcatggag aaactgacat caggacagcg cgccgccacc
     1441 cgtatgatac tggaaacgtc tgaccgtttc acggtggtgc agggctatgc cggtgtgggt
     1501 aagaccacac agttccgggc ggtgatgtca gccgtgaaca tgctgccgga gagtgaacgt
     1561 ccccgtgtcg tggggctggg gcccacacac cgtgcggtcg gggagatgcg cagcgccggc
     1621 gtggatgcac agacactggc gtcctttctg catgacacgc agcagcgcag cggagaaacg
     1681 ccggatttca gcaacacgct gttccttctc gatgagagct ctggtaatgc tgccaactta
     1741 ctgatttagt gtatgatg
//
LOCUS       DADLXX010000281         1746 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLXX010000281 DADLXX010000000
VERSION     DADLXX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.287"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            44..976
                     /locus_tag="JNX27_004192"
     CDS             44..976
                     /locus_tag="JNX27_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7885133.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1052..1126
                     /locus_tag="JNX27_004193"
     tRNA            1052..1126
                     /locus_tag="JNX27_004193"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Arg,seq:cct)
     gene            1288..>1746
                     /gene="intS"
                     /locus_tag="JNX27_004194"
     CDS             1288..>1746
                     /gene="intS"
                     /locus_tag="JNX27_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY7885134.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          492 a          398 c          438 g          418 t
ORIGIN      
        1 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
       61 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
      121 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
      181 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
      241 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgtcg aactggaagg
      301 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
      361 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
      421 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
      481 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
      541 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
      601 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
      661 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
      721 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
      781 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
      841 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
      901 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
      961 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
     1021 agtccattag cgggtatact catgccgcat tgtcctctta gttaaatgga tataacgagc
     1081 ccctcctaag ggctaattgc aggttcgatt cctgcagggg acaccattta tcagttcgct
     1141 cccatccgta ccagtccgca aaatcccctg aatatcaagc cttccgtata ttcacagttc
     1201 gtcatggttc gcgtcagatc gttgacagcc gcactccatg acgggtaaaa agtggataaa
     1261 ataattttac ccaccggatt tttacccatg ctcaccgtta agcagattga agcagcaaag
     1321 ccgaaagaaa aaccataccg ccttctcgat ggtaatggcc tgtaccttta tgtccctgta
     1381 tccgggaaaa aggtatggca gcttcgctac aagattgacg gtaaggagaa aatcctgacc
     1441 gtcggaaaat atccgcttat gactttgcag gaggcaaggg ataaagcatg gactgcgagg
     1501 aaagacatct cggctggcat cgatccggta aaggcgaaaa aggcttcgtc taacaacaat
     1561 tcatttagtg cgatttacaa ggaatggtac gaacacaaga agcaagtctg gtcagtaggg
     1621 tatgcgactg aacttgcaaa aatgtttgat gacgacattt tacccatcat cggcggcctt
     1681 gagattcagg atattgagcc gatgcaactg ctggaagtaa tccgcagatt tgaagatcgc
     1741 ggtgca
//
LOCUS       DADLXX010000282         1735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.288, whole genome shotgun sequence.
ACCESSION   DADLXX010000282 DADLXX010000000
VERSION     DADLXX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.288"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..166
                     /locus_tag="JNX27_004195"
     CDS             71..166
                     /locus_tag="JNX27_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAY7885135.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="JNX27_004196"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="JNX27_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAY7885136.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          642 a          288 c          278 g          527 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc gtcgt
//
LOCUS       DADLXX010000283         1731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLXX010000283 DADLXX010000000
VERSION     DADLXX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.289"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(219..773)
                     /locus_tag="JNX27_004197"
     CDS             complement(219..773)
                     /locus_tag="JNX27_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7885137.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(770..1060)
                     /locus_tag="JNX27_004198"
     CDS             complement(770..1060)
                     /locus_tag="JNX27_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7885138.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1060..1659)
                     /locus_tag="JNX27_004199"
     CDS             complement(1060..1659)
                     /locus_tag="JNX27_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7885139.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          415 a          477 c          394 g          445 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tggcgcgtga ggaatatagc cccggataac agattgagtc aacaaacggt ttttagatcc
      241 ccggaagaga atgcatcacg catcggtaga tagagcatat attcagcaag atttagccat
      301 acatctaccc gactgcagca cgtccggaaa caccactcag ggtgtgcgtc attcagcaat
      361 tcagccattt tacgcttagt catcccccgc ccttcatacc gttgccggag gacactaatc
      421 aatccaggat gctctgccag cacctcactt atgactctat caatacataa cgcctctgca
      481 tcagtacaat gcgccagcca ggtcttttgc ttgccattga tcatctctcg caaaaacgct
      541 tccagctcag gtttctctat tcccgctttt ttcatcctgc gcagggcttc attgacggct
      601 gttttcgtca attttttgga tgccagcaac tggttaaaca tatttcccgt cttaccgccg
      661 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      721 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cataaataac
      781 cttcctttct ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt
      841 cttctttggt gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg catgagctac
      901 aggcaatcgc tgcctgcata tcgtgtggtt ttgtcgctgt tccgcacgtc cccgccagcc
      961 tgtaatgcgc cagcacagaa gtttcgggat tgtgattgca gtagccagga attctgacgg
     1021 tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagtaactg
     1081 cgcggccaca ttttcgactt cctccggaga ggaaaattta cggaacagga tccagttcca
     1141 cagcacattc agtacagatt tataaacctg ctgaaactcg gtttcgtcca tgttcgcaaa
     1201 tgcgatagat tttgccctgc gcccacgact accatcagga taaatatgct cggtgtaaaa
     1261 tccggcctga atggttaccc actcgcggaa agcgtcaaac gactttagca atgccgtatc
     1321 ccgggttctg catgtcgcaa ctgtattaag gtattgctct gcggcatcac tcagggctgg
     1381 agtgtgttcc cgacctactg attcgcacag gtaatcaacg aagcctgata ccagttttcg
     1441 ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa tcaaatccca gttgcaggag
     1501 tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca
     1561 ctcacctatt ttgatttgat gcaaaaaatc gcaactctcc ggcgtcgccg ggagaagtaa
     1621 tccggaagag gtttgtttga ccagttgtat atgcgccatc gtagttctcc gctggcgcag
     1681 tagaatgggt gttcagcccg ttatgtagta taccagaatt aatgccaata c
//
LOCUS       DADLXX010000284         1726 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLXX010000284 DADLXX010000000
VERSION     DADLXX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.290"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..546)
                     /locus_tag="JNX27_004200"
     CDS             complement(<1..546)
                     /locus_tag="JNX27_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885140.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..>1726)
                     /locus_tag="JNX27_004201"
     CDS             complement(1208..>1726)
                     /locus_tag="JNX27_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885141.1"
                     /translation="SKMVDVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTESSF
                     GSEVTDYGIIAHESHLKALSRLAAKQGFGGEDAIVDMLGTDIAYYNGADRGVFMMAKR
                     FTALSFGCFRHDGENITVVLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVL
                     PVVKRLSFTKTS"
BASE COUNT          493 a          385 c          388 g          460 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagatta atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgcaccc acgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga tgtctgtacc cagcatatcg acaatagcgt
     1501 cttcgccacc gaagccctgc ttagcagcca gacgggaaag agccttgagg tgtgactcat
     1561 gggcgatgat accgtaatca gtaacctcag aaccgaaaga agactcggtg ttctcataca
     1621 gagcgtccat gatagtgtct gccttagcgg tcaggctacc agtcaggacc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttaga
//
LOCUS       DADLXX010000285         1694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLXX010000285 DADLXX010000000
VERSION     DADLXX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.292"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..69
                     /locus_tag="JNX27_004202"
                     /pseudo
     CDS             1..69
                     /locus_tag="JNX27_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(219..1079)
                     /locus_tag="JNX27_004203"
     CDS             complement(219..1079)
                     /locus_tag="JNX27_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7885142.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1262..1663)
                     /locus_tag="JNX27_004204"
     CDS             complement(1262..1663)
                     /locus_tag="JNX27_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7885143.1"
                     /translation="MKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDG
                     ISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTL
                     HQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          432 a          436 c          375 g          451 t
ORIGIN      
        1 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat
       61 ctctgctctc aggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      121 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      181 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
      241 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      301 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
      361 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
      421 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
      481 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
      541 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
      601 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      661 atcgttgtca gaagtaagtt ggcagcagtg ttatcactca tggttatggc agcactgcat
      721 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
      781 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
      841 gataataccg caccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
      901 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      961 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1021 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac gaaaatgttg aatactcata
     1081 ctcttccttt ttcaatatta ttgaagcatt taccagggtt attgtctcat gagcggatac
     1141 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1201 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1261 atcacgaggc cctttcgtct tcaagaattt tataaaccgt ggagcgggca atactgagct
     1321 gatgagcaat ttccgttgca ccagtgccct tctgatgaag cgtcagcacg acgttcctgt
     1381 ccacggtacg cctgcggcca aatttgattc ctttcagctt tgcttcctgt cggccctcat
     1441 tcgtgcgttc taggatcctc cggcgttcag cctgtgccac agccgacagg atggtgacca
     1501 ccatttgccc catatcaccg tcggtactga tcccgtcatc aatgaaccgg actgccacgc
     1561 cctgagcgtc aaattccttt atcagttgga tcatatcggc agtgtcgcgg ccaagacggt
     1621 cgagcttctt aaccagaatg acatcacctt cctccacctt catcctcagc aaatccagcc
     1681 cttcccggtc tgtt
//
LOCUS       DADLXX010000286         1679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLXX010000286 DADLXX010000000
VERSION     DADLXX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.293"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(72..539)
                     /locus_tag="JNX27_004205"
     CDS             complement(72..539)
                     /locus_tag="JNX27_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY7885144.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFFALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="JNX27_004206"
     CDS             complement(605..844)
                     /locus_tag="JNX27_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY7885145.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="JNX27_004207"
     CDS             complement(979..1584)
                     /locus_tag="JNX27_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7885146.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          543 a          369 c          294 g          473 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc aaaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DADLXX010000287         1662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLXX010000287 DADLXX010000000
VERSION     DADLXX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.294"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..933)
                     /locus_tag="JNX27_004208"
                     /pseudo
     CDS             complement(1..933)
                     /locus_tag="JNX27_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(961..>1662)
                     /locus_tag="JNX27_004209"
     CDS             complement(961..>1662)
                     /locus_tag="JNX27_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7885147.1"
                     /translation="ESWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLS
                     PEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGMQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          519 a          366 c          328 g          449 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagaccatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt ggaaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaatacctg acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aagtgtacaa ccaccaatat acagcgtcag
      661 gccaattaaa attgccacct tataactggt ctttttaatc acaagggatg ctggtattgc
      721 aattaaaaaa taacctccat aaaatgcgct ctgcaccaat gctgaagcaa agttacttag
      781 cgaaaataca cttttgaatt gagtgattaa tatgtcattt aatgcagctg cgcatcccca
      841 tagcgggaat aaacacgata acaaaataaa ctggaacaag ggagtcttat tcagataccc
      901 atcaggcatc tgaatgatgt ttttatcgtt catagtgcta cctttaactg tgcaggatga
      961 ttattcgttt aaggttaaaa attcattaaa ttgttcaata ctcggataag atgattgcat
     1021 acctttccct gtgacgctga aagcggcaaa gagagcggct tttttcaaag cggcttcaac
     1081 atcaccgctt tgaacataat aatgggaaaa gcaaccaata aatgcgtcac cagcgccact
     1141 agtatcaaca gcatttactt tgaatgcagg aacatggact tcctgatcgc gggtcatcca
     1201 taatgcgcct ttttcgctca tggtaacaat aatattgttc agccctttat caactaacga
     1261 acgtgcggcc aaacgaatat gatcataagt atcaaccgac ataccggtta atatttccag
     1321 ttctgtttca ttcgggataa agaaatcacc tttgcaggca taagacatat ctaactcacg
     1381 caatgccgga gccggattta ataacacttc aataccattt ttcttaccaa actcaatcgc
     1441 gtggtaaact gtttccagtt gaacttccag ttgtaaaacg atcaatttgc attttttcag
     1501 atcttctgca gctcgatcga tatcttccgg ggaaagaaat ttattcgctc ccttaattat
     1561 taatatacta ttgctcgagt tggcattaac aaagatcggt gcaacaccac tgctggtaca
     1621 ggggaccttc tcaacataag tggtattaat tccccatgat tc
//
LOCUS       DADLXX010000288         1654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLXX010000288 DADLXX010000000
VERSION     DADLXX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.295"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(155..1390)
                     /locus_tag="JNX27_004210"
     CDS             complement(155..1390)
                     /locus_tag="JNX27_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7885148.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1537..1613)
                     /locus_tag="JNX27_004211"
     tRNA            complement(1537..1613)
                     /locus_tag="JNX27_004211"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:tgg)
BASE COUNT          422 a          452 c          391 g          389 t
ORIGIN      
        1 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg
       61 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
      121 atcaaccgca ggcggcttcc aaatggccgc ttttttactt attcaccacc atcaaatgtg
      181 cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat
      241 atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt
      301 tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
      361 cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct
      421 gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt
      481 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
      541 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
      601 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcaaaaa
      661 tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc agttccccct
      721 gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca tcagccaggc
      781 gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa
      841 cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat
      901 gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
      961 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
     1021 aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg ttagtctgga
     1081 aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt
     1141 aaaactccag cccgagtagc gttggttcgc caccctgcca agtaaaagcc acttcgtttt
     1201 gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat
     1261 gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt
     1321 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
     1381 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat
     1441 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca
     1501 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
     1561 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
     1621 gctttttgaa tttttagttc aattctttaa agtc
//
LOCUS       DADLXX010000289         1649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLXX010000289 DADLXX010000000
VERSION     DADLXX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.296"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..999)
                     /gene="capU"
                     /locus_tag="JNX27_004212"
     CDS             complement(<1..999)
                     /gene="capU"
                     /locus_tag="JNX27_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7885149.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPTNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQ"
     gene            complement(1002..>1649)
                     /locus_tag="JNX27_004213"
     CDS             complement(1002..>1649)
                     /locus_tag="JNX27_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7885150.1"
                     /translation="PRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLITGFIGNGP
                     VRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTHTRWDKKF
                     SSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELGFYYLYTT
                     ERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPRLPDD"
BASE COUNT          488 a          391 c          299 g          471 t
ORIGIN      
        1 ctgataaaaa cgcccagggt cattaaaaaa atcattcagg gcgcacatcc atgcgtgctt
       61 attacctgct ggtaacaatg tcccggtctg gttgttctga ataacatcag ggattccacc
      121 aatctgactg gccagtacag gcacagaaaa tgccgatgct tctgccagta ccataccaaa
      181 cgattcgttt tctgaaggca gaaccaccag actggcaacc cgatatacgg gggcggcagg
      241 aaaaacattg tcagcaataa aaacatcatc atgcatgccc atactgtcaa tctgatactg
      301 taaatgctcc cgcagttcag gcgaaccaga ccccacaatc aaccaacaga actgtcgtcc
      361 attcattttt aaatggaaca gtaaattcaa cataaattcg tgcccttttt ccgggcgcaa
      421 cattcctacc tgagcaataa cggggcatcc ccttcgggac gccagccaag aaagaacatt
      481 tgtaggcaaa ctgtttcggg aatcaacgta taatttctgg aaatcaaagc ctggaggcac
      541 aacagtaacc cgggttcgac atccttcctg ctccagatgt gtcttcatac ttgttccggg
      601 aacaatcacc tcatcgcaaa aatgatttat tgaaaaaact tttgtttttc gtgtcaaata
      661 tgtcttttgc ctgataattc tgaatggatg tttcctagtg aataaccgta ctaaaccaac
      721 aatattgcta tcatgcccac tgtgacaaac gattgcattg ggctgaaaac catgaactat
      781 tccgagtaat ctccatgcag tagggatgtg aagactgttt cgaaataacg cgaatgtgat
      841 atcaattccc aatttactgg cttcaaaagc aattttgctg ttttccctgc agacaagcag
      901 aactgaatgc cccattttct tcagggcctt catttgagca acagcctgta attcctgacc
      961 acctatattc ggtgatgatt ccgtaaatag tatattcatt attaatcatc cggtaatctt
     1021 ggccccttat gaaaggccag caacgatgaa aaaaacggtg ttgtataata aaaaagtcgt
     1081 cttttcagcc atgcgcaact ttcccgttct ttggtgctta ttcttcctat ccgtgtagtt
     1141 cctttagcag gagcattcat tcttctttct gttgtatata aataatagaa tccaagttct
     1201 tcagcgacct gaatataatc cttgttataa taaccttccg gccaacacaa atgtttactg
     1261 cattttcctg tcatttcttt caaacattct ctcccgctca acaagtcctg ccgaagatgc
     1321 ttacactgtt cttctcgtga actgaacttt ttatcccagc gtgtatgagt atgcgtatga
     1381 acatgaaatt cgaccagacc agattgcaac atttcgttta cttcagacca gcgaagcata
     1441 acattatcag cattccctgt cgctatttga tgttcgcaat cgcgatgaga atattccttt
     1501 ccaggagagt gacgaaccgg accattccca ataaaaccgg taattaaaaa aatatgtgct
     1561 ttgagattaa actcttttaa taaaggatat acctgaaacc agttgtcaag gtatccatca
     1621 tcaaatgtca acataacgct tttccgggg
//
LOCUS       DADLXX010000290         1607 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLXX010000290 DADLXX010000000
VERSION     DADLXX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.297"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>1607)
                     /locus_tag="JNX27_004214"
     CDS             complement(<1..>1607)
                     /locus_tag="JNX27_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7885151.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESG"
BASE COUNT          413 a          416 c          347 g          431 t
ORIGIN      
        1 atccgctctc actttctggt gtcgaaagtg aataattgaa gatgcatttt gttgaggcgt
       61 tttctcccca ggataacgta acctgtcctt tttctggtag accagaaaga taaagttcgc
      121 cacggtcacc gacaattcct gaatctgcca aacttgcatc ttgtatcgca gcaatggctc
      181 caaaaggtac cggtttacca tttgcattta acactctgaa gagaacgcga taacccacat
      241 gagtcacaaa ctccgcttta accactgcac cacgcgtggg aaccatcgta acgacggtat
      301 tatcaagttc tatattcgac ttcgagaaat agttatcgct gattgctacc cgattttcat
      361 cataagggtt tagctgtgtc ttgaccgtat agccgcgcca gtccgtcgca gcaccgcgca
      421 tattatctat ttctaacccg gcggccccag gtgctttaac aagagcaata gtttcaccta
      481 actcctgact taacgttacc ccttcgctgt gtgccagaat tccccctctg ataccataat
      541 tcagcctctg ggaattttgg ctgtagttat agctgccgtt caccgtgcca taagagccat
      601 cataggtcag ggacatatta ccgccaacct catcatcatg actgttatag gactgcatta
      661 aattccatga aaggttattt ctttccagcg ccgtaccgct gagtccgagt tgatgtgagc
      721 taccggaatt atcgttttgc gaaacagaat aggtggcgta actattaccc caccagcccg
      781 ataaaggaac agataaagag agcatgtaga tctgctcact ggtgttatca ctggcatggt
      841 tgttccccgt cccctgtctg gcagtattgg aataactcca ggagacgttc cacgagagac
      901 gcttccagct actgctgtag ccaaattgca ttaatcgttc gacatcatcg gtatgccagt
      961 attgttgccg gcttaatgtc aggtataaat tgccataatc tctccccaac gactgcgtta
     1021 gcgttccctc cacgcgacta cgtcggttac ctgtttccca aaagtcttca gggctgttgc
     1081 gccgcgatgc ccactcattg agggtatagt agccctcggt ggagtaacga tagccaacca
     1141 ggcgcaagct agtgtctgtg tcgtcaaata gttttgaata gagaaagcga tatgactgac
     1201 ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg tgtcacatcg aaagaaattg
     1261 cgcccaaagc gccaagatct ttaccgacac ccagagctgc agaaccataa ttttcagcag
     1321 cctgcatacc tccatataaa gtgatcccgt gggaaaaacc atgaagtatt tgcgcctgaa
     1381 ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc cacgctgaca tcaacatctg
     1441 tcagaccttc acgtttaaga atcgccaatg aagcataagg ttgggtgaat cgttgttcgc
     1501 taccgtcttc ttctttgatg gttacgtcca aatcgccacc tgtacttgcg gtgttgagat
     1561 cggtgatctc aaaagcacct tgtggaacgg ctgactgata aatcacg
//
LOCUS       DADLXX010000291         1587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLXX010000291 DADLXX010000000
VERSION     DADLXX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.298"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..217
                     /locus_tag="JNX27_004215"
     CDS             <1..217
                     /locus_tag="JNX27_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885152.1"
                     /translation="AEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            425..694
                     /locus_tag="JNX27_004216"
     CDS             425..694
                     /locus_tag="JNX27_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885153.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            691..803
                     /locus_tag="JNX27_004217"
                     /pseudo
     CDS             691..803
                     /locus_tag="JNX27_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            946..1536
                     /gene="ospG"
                     /locus_tag="JNX27_004218"
     CDS             946..1536
                     /gene="ospG"
                     /locus_tag="JNX27_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY7885154.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          456 a          312 c          406 g          413 t
ORIGIN      
        1 ggcagaagag cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg
       61 gctgaaagca tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt
      121 gatgcgtgag actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg
      181 attgtctgaa aacggctcac aactgcacca ttcataatca catcgcataa aactgtcagc
      241 gccagtgata ggacaggtga aacctgtcaa tacgtaccgg cttacgccgg gcttcggcgg
      301 ttttactcct ctgccatatg caacaacgga gtgccgcctt acatgccgct ggcgcggcat
      361 atcctggtga cgatatctga acagttatat actgtgtata tacgtggtaa tgacgaggtg
      421 gcaaatggca caggttaata tgagtgtaag aatcgacgca gaactgaagg atgcttttat
      481 ggccgctgca aaaagcatgg accgtaatgg ctctcagtta atccgggatt ttatgcgcca
      541 gaccgttgaa cggcagcaca attcttggtt ccgtgaccag gttgcggcag gccgtcagca
      601 actcgaatgc ggtgatgtgc ttccccatga catggtcgaa tcctctgctg ctgcatggcg
      661 ggatgaaatg agcaggaaga ttgctgataa atgatggaga tgttctggac catgctggcc
      721 atccaggaca ggaagcgcat tcgtagtaca ttgcggagca gaacctgatg gccgccattg
      781 aaccggacga acggattggt tattcagcca gcagcctggc agggcagccg tataaaggcc
      841 gcaacggtcg tgtggaagga acatctggac cacacaaggt agcgtgtaac gttattttgt
      901 gtgaaaatct cttgtgattt ttaaatatcg taacaaaggt atgacatgaa aataacatct
      961 accattattc aaacaccttt tccatttgag aataataatt ctcatgctgg catagtaacg
     1021 gagcccattc tcggtaagtt aataggtcag gggtcgacag cagaaatctt tgaagatgtg
     1081 aatgattcat ctgctttgta taaaaagtat gatcttattg gcaaccagta caatgagatt
     1141 ctggaaatgg cttggcaaga atctgagctt tttaatgctt tttatggcga tgaagcatcc
     1201 gttgttatac agtatggcgg agatgtgtac ctccgaatgc tgcgcgtgcc tgggactccc
     1261 cttagtgaca ttgatacagc tgatatccct gataatatag agagccttta tctacagttg
     1321 atatgtaaat tgaatgagtt gagtataatc cattacgatc ttaatacagg taatatgctg
     1381 tatgataaag aaagtgaaag tttattccca atagattttc gcaatattta tgctgaatat
     1441 tacgctgcaa ccaaaaaaga taaagagatt atcgaccgac gattacaaat gcgtacaaat
     1501 gatttttatt cgttattaaa caggaaatat ttatagccgt atttgttgat gctataagcc
     1561 agaggctctt ctatgagaat atgggga
//
LOCUS       DADLXX010000292         1549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLXX010000292 DADLXX010000000
VERSION     DADLXX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.299"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..265)
                     /locus_tag="JNX27_004219"
     CDS             complement(<1..265)
                     /locus_tag="JNX27_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885155.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMF"
     gene            complement(330..1280)
                     /gene="virK"
                     /locus_tag="JNX27_004220"
     CDS             complement(330..1280)
                     /gene="virK"
                     /locus_tag="JNX27_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7885156.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            complement(1285..>1549)
                     /locus_tag="JNX27_004221"
     CDS             complement(1285..>1549)
                     /locus_tag="JNX27_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7885157.1"
                     /translation="MVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMC
                     ALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          472 a          340 c          290 g          447 t
ORIGIN      
        1 caaacatttt gtcgacaatt atttcccgtt ctgtatcact cttttctggg aaacaaagcg
       61 ataaattaat cgtcgccctg cgacgagctt ttttgctcaa tctgcccgcc caacgcccta
      121 tttttgccaa gacaggatct ctgaatgaag ggggaaacat tgaaatacct gcaatcattc
      181 ccaaaaggaa ccatgtagac cagtaaacag gggaaaggta atttttctta aattcaggaa
      241 taaactcaga tttatatttt ttcattattt agtgcgaatg cctctaccaa aattatcatt
      301 actgttttat tcagagaata catcaaacct taatttaaat tctgatgttc tgagtgtaca
      361 ggatatgcat ccaaagagcg gatagtagtt tcatggatat aatccagcaa agcgtatcgt
      421 tttcggtact cagagcgttt tttactggct atatcgctta gcggctttcg aatcacctgg
      481 gttggtaatc tgtaccagtc accacaggtt tttcctgcta cagactccca gaaatcacta
      541 tagaccgcaa caaaggtttt gcgtttctga taccagtatc tcaattgccg gaaaacatga
      601 ctgtgctcac tgactgccag acactcagag atattgcaag ctttcatcaa cgcacaaaat
      661 gcttcaaaaa taatccgttt tgggaaaatc ccataacagg cccgggttgc gcaccgaata
      721 acatcaggtc cggtattttt aggggctccc tgtaatccgc caataaaagc aatgtgtcca
      781 ttttgagtca gaatgactga aaatgttaac cgggcaataa ctgttttatt gtaaaacaat
      841 gacaacatca actctccttc ccggtcaaaa ccactgggag aacactggat atcaaaattc
      901 tctccatctt tcccgatgaa ttggactaat gataccgatt tttgtggaaa caaaaattta
      961 gaatattttt ctggcaaatt ttgcacaaag cgataatgct caagaatata ttgtccacgg
     1021 gtccaggcgc gccccccttt atgcaaataa gggcgatgaa ttcttgctgg caacgtgggg
     1081 tgtattgcca gaatatcctc aatgcacggc aaggaacgca gttcataata atatcgaata
     1141 ctgacaaccg gatttataga acatcgcaaa atgtacatga accgaaattt tctgtgttcc
     1201 cacttccccg gtagtgaatc tgaggaaaaa ataatccttt taaggaaacc gataaatgat
     1261 aagttactta tagaaaacat attgttactt ttgcttcgct aatgtgagta tttttaacgc
     1321 agttttatta atatcaaacc gctcttctat atcctgtttt gccaggcgag ccatctgata
     1381 aaaacgccca gggtcattaa aaaaatcatt cagggcgcac atccatgcgt gcttattact
     1441 tgctggtaac aatgtcccgg tctggttgtt ctgaataacc tcagggattc caccaatctg
     1501 agtggccact acaggcacag aaaatgccga tgcttctgcc agcaccata
//
LOCUS       DADLXX010000293         1541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLXX010000293 DADLXX010000000
VERSION     DADLXX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.300"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(421..612)
                     /locus_tag="JNX27_004222"
     CDS             complement(421..612)
                     /locus_tag="JNX27_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7885158.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            677..1000
                     /locus_tag="JNX27_004223"
     CDS             677..1000
                     /locus_tag="JNX27_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY7885159.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            990..>1541
                     /locus_tag="JNX27_004224"
     CDS             990..>1541
                     /locus_tag="JNX27_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY7885160.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAESVTRGIDALYHV"
BASE COUNT          329 a          407 c          469 g          336 t
ORIGIN      
        1 gaaaaacgcc agcggcgcag cctttctttg ccctttgcct ttttgctcac atgttctttc
       61 cggccttatc ccctgattct gtggatcacc gtattaccgt ctttgagtgc gctgataccg
      121 ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc
      181 ggaagctgca ttttctcctt acgcatctgt gcggcatttc acatcggcta tggtgcactt
      241 ttcatacaat ccgcactgat gccgcataag taagcctgtg tacactccgc tatcgctacg
      301 tgactgggtc atggctgcgc cccgacaccc gctaaaacct gctgacgcgc cctgacgggc
      361 ttgtctgctc ccggcatccg ctcacagaca agctgtgacc gtctccggga gctgcatatg
      421 tcagaggttt tcaccgtcat ccccgaaacg tgcgaggcag ctgcggtaaa gctcatcggc
      481 gtggtcgtga agcgattcac agatatcagc ctgttcatcc gcgtccagtt cgttgagctt
      541 ctccagcagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
      601 ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttttatgggg taatgatacc
      661 gatgaatcgc gagaggatgc tcaccatacg ggttactgat gatgaacatg cccggttact
      721 ggaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc tgggtgagcc
      781 ggttgcccgt tccgggaaac tgccgacact ggcaccgccg ttactgcgtc agctggctgc
      841 catcgggaat aacctgaatc agacagcgag gaaggtgaac agcgggcagt ggtcttccgg
      901 taaccgggtt caggtggtgg ccgcactgat ggccatcggg gacgagctgc gccggctgcg
      961 tctggccgtc agggaacagg ggacgcggga tgatagttaa gtttcatgcc cggggaaaag
     1021 gtggtggcag tggtccggtt gattacctgc tgggaaggga gcgtaaccgg gaaggggcaa
     1081 cggtgcttca gggtaatccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga
     1141 agaaatacac gtccggtgtt ctgtcgttcg cggagaagga gctgacgccg ggaggacgtg
     1201 aaaaagtgat ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca
     1261 gcatcctgtg ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga
     1321 acatggagct acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta
     1381 gaattgatgc ctggcagacg ctggttaatc accattacgg gctgcatgac ccgaacgccc
     1441 cggagaaccg caggacgctg acactccctg ataatctgcc ggaaacgaaa caggcgcttg
     1501 cagagagcgt cacacgcggg atagacgctc tttatcatgt c
//
LOCUS       DADLXX010000294         1459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLXX010000294 DADLXX010000000
VERSION     DADLXX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.301"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..507
                     /locus_tag="JNX27_004225"
     rRNA            <1..507
                     /locus_tag="JNX27_004225"
                     /product="16S ribosomal RNA"
     gene            564..640
                     /locus_tag="JNX27_004226"
     tRNA            564..640
                     /locus_tag="JNX27_004226"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:598..600,aa:Ile,seq:gat)
     gene            683..758
                     /locus_tag="JNX27_004227"
     tRNA            683..758
                     /locus_tag="JNX27_004227"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:716..718,aa:Ala,seq:tgc)
     gene            933..>1459
                     /locus_tag="JNX27_004228"
     rRNA            933..>1459
                     /locus_tag="JNX27_004228"
                     /product="23S ribosomal RNA"
BASE COUNT          401 a          327 c          414 g          317 t
ORIGIN      
        1 gtgctgcatg gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc
       61 gcaaccctta tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga
      121 taaactggag gaaggtgggg atgacgtcaa gtcatcatgg cccttacgac cagggctaca
      181 cacgtgctac aatggcgcat acaaagagaa gcgacctcgc gagagcaagc ggacctcata
      241 aagtgcgtcg tagtccggat tggagtctgc aactcgactc catgaagtcg gaatcgctag
      301 taatcgtgga tcagaatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc
      361 acaccatggg agtgggttgc aaaagaagta ggtagcttaa ccttcgggag ggcgcttacc
      421 actttgtgat tcatgactgg ggtgaagtcg taacaaggta accgtagggg aacctgcggt
      481 tggatcacct ccttacctta aagaagcgtt ctttgtagtg ctcacacaga ttgtctgatg
      541 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
      601 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      661 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      721 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      781 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      841 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagtctct caaattttcg
      901 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      961 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
     1021 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
     1081 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
     1141 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
     1201 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
     1261 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
     1321 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
     1381 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
     1441 gaaaaagaac ctgaaaccg
//
LOCUS       DADLXX010000295         1444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLXX010000295 DADLXX010000000
VERSION     DADLXX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.302"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1155)
                     /locus_tag="JNX27_004229"
     CDS             complement(<1..1155)
                     /locus_tag="JNX27_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY7885161.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIH"
     gene            complement(1155..>1444)
                     /locus_tag="JNX27_004230"
     CDS             complement(1155..>1444)
                     /locus_tag="JNX27_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HAY7885162.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          278 a          479 c          334 g          353 t
ORIGIN      
        1 atgaattcct gatgtgaaca gccccttctc acggtcgagg gggataagct gctcacggct
       61 gatggcctca tcgataccgg cgcgtgcccg ttcaatcaca ccattttccg gcggcagtat
      121 gccgaccgtc ctggccagca cgtccgtata cgtgaactgc acttttcgtt cacttaatcc
      181 ggcaatcgcc tgtgtcaccg cctgctgcac atccggcccg tcctgtgaag caggcccggg
      241 cgcctgcgtg cggatctccg cacgctgatc cgccgcgtca cgatatgccc ggatgtcgaa
      301 cccggtttcc ttcagcgtct gcatccattc agccattctg atttccggat cgacgtgctg
      361 tttggatttt cgcgtatcca gtgccgccac atcccgggat ttcagcgagg cgtcttcacc
      421 gacggcctcc ctgatagcct gtgaacgact ggaaaaggcc tccaccggta cgcccggcat
      481 ctcccacata ccgtgcttac cgaccacttc ggtttcataa cccagcgcct caacgcgctg
      541 acgtaactca ctctgatata ttttcccgaa cgcaatccgg ttcgcgagca cgttctcgga
      601 aaatccggtt ttccccactt tgtcactgct cagtgtcttc cactcgccgt tatgctgcgt
      661 gacattagcc accaccacat gcgtgtgtaa ctgtgggtcc tgatcgcgac tggtgtcatg
      721 gttaaacagt gccatcacca gattaccggt cagcaccgtt tctgactgtc cgtccgtcat
      781 cacccgtgtg gaagccagcg cctccacctg acggacagca aaatccacgg cctggttatg
      841 tgcatcaata agacgtttat cccccccgag catggccatc atggagacac ttttgggggc
      901 ggagaaggtc aggtcgtagc cgggacgatg cttattactg ccatcctgca tgcggcttaa
      961 atccgccccg tccggcagcc tgccctccag aagacgggta aaaacatcct tatcgacgct
     1021 gccctgcagc ccaagctgtt cagccccctg accggcccag cgttctccca tgctgcccag
     1081 cacatagtaa ttatccttgt cggtgtaata gtttccggca ctgcctgccg atttaacaac
     1141 gctgaaactc aacatcagaa atcatctccc ggctcaacat cctccccgcg ctcccggtgc
     1201 acattaatgt tcacctcttc acgacgctgc atatgctgct ggatgtccgg atgattttcc
     1261 tgttgccatg cctcataagc ggccatatcc accacttcac cggattcact gatcccgggt
     1321 ggcagttgct gttccatctc ttcttccggt ttcattttca gctcctgctc gatcccccct
     1381 gccggaacac tcacacctgc atctgacttc ttatcattga tgacagaaga cactggctgt
     1441 tgtg
//
LOCUS       DADLXX010000296         1398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLXX010000296 DADLXX010000000
VERSION     DADLXX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.303"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..998)
                     /locus_tag="JNX27_004231"
     CDS             complement(<1..998)
                     /locus_tag="JNX27_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7885163.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQRGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          420 a          222 c          294 g          462 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccctctggc gtaaaaacag tctgttttgg tctgacaggg aaatacgaaa ctgaggcccg
      181 ttaaaatttg gaaccgaagt ttgctgtatt aagaatagca gagtttgatc tgataagggg
      241 ttatttgcaa tctcaatagt tatatgagga tctaaacgaa aaagatttgt cgggattgta
      301 gttatttggt tgttttcaaa ataaaaaaat cttctcatga taatattttc ttggatgttt
      361 ggtaagttgc ttaatctatt gctatatgca aaaagaagtt gcagtctcct aggtaaaggg
      421 ggcagttcag taagatggtt gtgcgcaacc ttaagtatct taagattaga aggcagagat
      481 ggaagagttt gcagttcgtt gttcctaacc tcaagttttt ttaaattcaa gggtaatgaa
      541 ggcagtgtgg ataacctatt atactctgcg cttagtgatt tgatggtgtc tggtaataac
      601 ggcagattct ctaattgatt ttcggacaca tccaaagtta ttagataatg tggtaatgca
      661 gatatatgtg ttaagaaatt atctcttgca ataagttctt taatattttc aggtaaatca
      721 gtaaggatgg ttaatttatt acagctcaca tcaagctttt taatgcaagc agggagtaca
      781 ggtaacgagg aaagcgccaa ggctcgcagg tccagtttat gccccttaga ggtaagacat
      841 gcacgaagtc ttcttaccgc ctcagtacgc tgttcttccg gtaacccctg tttctcccaa
      901 tcatcccatt cggacaaata atggttgggg gatgtaattg tgctgatatg tggaaataaa
      961 caggatgaaa tatttctgag catattaatt tctcgcatat ggatatggaa ttagattgtg
     1021 aataaattgt atttaatgta tctctcctaa tgaggagggt gtttcagaat attcgccata
     1081 agggaatgag tgttatatca tgctttctac cctggtaagt atatttgctt ttttcgtata
     1141 tttggttcat ctttttttga tgttttacat attcacaatg cagaggattt ataccagaag
     1201 acattagtct tgagtaagat actacaacat ctccaactat tttagccagc ctgactactt
     1261 gacttggtaa tatttgttgc taataatcat gtataaaata agcttatata catagtaact
     1321 tcagcaaagg atttagtaat gatccaaatc atatctcaat gtactgtttt tttctttaaa
     1381 aaaacagtac attctatt
//
LOCUS       DADLXX010000297         1383 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLXX010000297 DADLXX010000000
VERSION     DADLXX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.304"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..569)
                     /locus_tag="JNX27_004232"
                     /pseudo
     CDS             complement(1..569)
                     /locus_tag="JNX27_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(617..1111)
                     /locus_tag="JNX27_004233"
     CDS             complement(617..1111)
                     /locus_tag="JNX27_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7885164.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          422 a          278 c          261 g          422 t
ORIGIN      
        1 acactgatat gaattgggtt gggattctct gctatcagat ttcgcccttc ccggcgccat
       61 ttcactttcg gcgcagcatc ttcgggaata aggcggatat ttgctggtcg ccagaacagc
      121 ttgatacgcg tttttactgc tatttccagc gagttcgtcg ctttgttgct cgctgatggc
      181 ggaatatttt taatattcag ccagaagact gactcgcgat catcaggcaa tgacacacct
      241 ttggtgtgaa taatgcgtaa tattttttct tgcgaaggtt ctaatcgtga tactggcgga
      301 ataacggtaa acggcgcttt gcttttatcc tctttattaa atggatctac ccaagcctgg
      361 atgagataag gtattttttc ttctttgcta aatacagaaa tagatttatc agcattatta
      421 cttaagtaaa ccacccgcgt actaccaatg acaacgcctg cataagttaa tgctggttgg
      481 cctaaaaaaa cgctgagtat aatcgcggta aataacttac attaaactgt tgttttaaag
      541 agcctgatta aacaactttt tctggacata acgactccct ggccaaagcc aattgaataa
      601 aaaggccctt gataaattat ttcttatatt cgacagtaaa ttgtgaatca gcgttcgccg
      661 taccagccgt tacggtcgca ttggttgcca catagcgtgc aataaacttc atttcagcct
      721 tgccttcagc atcgatagcg gtataatcag agtcgttata aagtttaacc tgcgaattat
      781 cggactggtt aaaaatacga ataccaacgc cggttgccgc aatcgcttgt cctgtaccat
      841 caaaacgaac ggctgcttga gtgtacgttg ccgggcaatt ttcaagttta atactgaact
      901 tctgtggcga cgccagacta cctacacctg aaaatgcatt tttactgact ttacccagtg
      961 ggacaatttg atcggctgta tcggaggtaa cttcacaagt agtgtcgaca atttcaccga
     1021 cgaagttaac aacaccatct tctgccagtg catgcccggc cagcattaga gatgccagag
     1081 tgaatgcacc gcctataata ttacgcttca taaaaactcc attgatataa tatatccttt
     1141 accatgaatc atcacttata ttaataagtg tatttttgat tcaaaggaat agtacaccaa
     1201 agagacggct ttatctaaaa ataatattaa ttacgcaaaa ataaaatata gatttattgt
     1261 ttaaatatta tttaaacagt aaaaaatcaa gtttgttatc agacattaat gataattcag
     1321 aatatattta tatagaaaga tagatgaatt tattgatgtc agttataaaa aagggccacc
     1381 ggt
//
LOCUS       DADLXX010000298         1370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLXX010000298 DADLXX010000000
VERSION     DADLXX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.305"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..704)
                     /locus_tag="JNX27_004234"
     CDS             complement(<1..704)
                     /locus_tag="JNX27_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885165.1"
                     /translation="MGPLPRKGNMKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRL
                     ITQRRKKHWDESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWS
                     SDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGF
                     EDFFIAVDPKTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLL
                     AMVDVLLCYLEKGAEYV"
     gene            complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNX27_004235"
     CDS             complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNX27_004235"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7885166.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1278..>1370)
                     /locus_tag="JNX27_004236"
     CDS             complement(1278..>1370)
                     /locus_tag="JNX27_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein D"
                     /protein_id="HAY7885167.1"
                     /translation="ARRPLKMLLAVALEPLLSRAANKLAQRYKR"
BASE COUNT          383 a          315 c          298 g          374 t
ORIGIN      
        1 acatactcag cacctttctc aaggtaacag agtaaaacat ccaccatcgc cagcaacact
       61 tcagggctac ggtaattcag gtcaatttga tcgtcactaa aggtggtcca taaatggcgc
      121 gtggaattat catccatttg aaacggcgtt aatagcggta acgcacgcgg acgggtgacg
      181 gcactgagat cggttttcgg atcaaccgca ataaagaaat cttcaaaacc cggaagctgt
      241 tgtaaatagt ttttaaacca ttcacttttt gcagacatat ggttgcagac aaaatcaaac
      301 attaaatgac tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
      361 acctgatgat aatcaattac cgaaaatcca tcatcagatg accatgggta aaaaggtaat
      421 aaatgaacat gtgaaaaaat gctttgcagc cattgatggt agaactgatt aaatatgggt
      481 agtggtttta aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt
      541 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
      601 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc
      661 gtaattttct gtttcatgtt gcctttccgt ggtagcggtc ccatgaggcg agagactact
      721 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
      781 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatagggat gtaaataagg
      841 tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
      901 cttcggcatt gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc
      961 gctaaggatc tcttttgcct gcccggactg gcgtccggca ttgcaataca ctttcacggt
     1021 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
     1081 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
     1141 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
     1201 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
     1261 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
     1321 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc
//
LOCUS       DADLXX010000299         1350 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLXX010000299 DADLXX010000000
VERSION     DADLXX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.306"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..328)
                     /locus_tag="JNX27_004237"
     CDS             complement(<1..328)
                     /locus_tag="JNX27_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7885168.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENI"
     gene            574..1335
                     /locus_tag="JNX27_004238"
     CDS             574..1335
                     /locus_tag="JNX27_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7885169.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
BASE COUNT          480 a          262 c          228 g          380 t
ORIGIN      
        1 ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa
       61 caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg
      121 acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc
      181 caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctggc aaatttatct
      241 catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat
      301 tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat
      361 tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa
      421 agtcatataa aacttatatc cacagaataa aaaaccaaac atgtcagttt tatcaatata
      481 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
      541 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
      601 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
      661 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
      721 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
      781 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
      841 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
      901 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
      961 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
     1021 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
     1081 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
     1141 gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
     1201 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
     1261 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
     1321 attcagttca actgatgtaa aaatcaccct
//
LOCUS       DADLXX010000300         1349 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLXX010000300 DADLXX010000000
VERSION     DADLXX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.307"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1013)
                     /gene="ospC2"
                     /locus_tag="JNX27_004239"
     CDS             complement(<1..1013)
                     /gene="ospC2"
                     /locus_tag="JNX27_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7885170.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDN"
BASE COUNT          442 a          213 c          219 g          475 t
ORIGIN      
        1 aagttatctg tgcttagcat tcttggtata tgatactcaa gctgaaacac aaagtttata
       61 attctatcca gactaacagg atcaatgttt ttattataac aaaactctcg gaatctttga
      121 tctttactct ttcttataaa atcaataaga tgcaacccaa ttccaagcct catatctttt
      181 gtattaaata ttggcacatc agtttttccc tgattaccat gcacatacct gctaacttca
      241 tccacaacct ctttaaagtt atctaagaaa aatgattggt gctcatggta aaaaacaggt
      301 ggaatagcat tatcaaagtg gtcggttaat gtcatatatc catatggaga gtcatgatca
      361 atgatatacg cattagcacc aaaatccaca gtatgatgtg ttttatttag tgggagtgtt
      421 tcttgatgat tggaaatttc aaccccaaaa aaaacaaaat cgtggttagc tatttcttca
      481 atatcctcag agaatgtgtt tttatatgtt tgtatattct cagacttaat tttatcaaag
      541 gacttaatat taagagtatt atttgacagg ttagcgttac tttgatgtct gaagttaaaa
      601 tcagaattaa taataataga gagcaagtga cgttcatccg aagaaagagg atgagttttt
      661 tgtattattt cagaaagata gtgtctggac accttatcga ggtgctcaag tgaccgaaat
      721 gatgaccgtg ctccagatgg cgcttcaata gaaagattta atgatttgaa actggtccct
      781 tgagagaaca ttttactata actttgcgct gctatagact tccttaaaaa gcttttcaca
      841 gtatcagcac agtgatctaa atgtttttca cttatgccag aagaggatga gtttgttacg
      901 catgatatgt ttttttgtaa tgcttttgta tctttgttcg gatttatttt tccatcaaca
      961 agatcaatat tgttctgaac attaatatga tttactgctt caggtatttt cataaaacat
     1021 cccccattat gtgcgttttt atctgcagtt atttagaata atattatcta acttaatatt
     1081 ttcttgtatg tattataacg aacatatttt aatatgttgt acgattttca ctctgaaatc
     1141 ccctgagttt aatcacactt cctttactga aaggaattat tgcaacagta ttatttgttt
     1201 ttggatatat atcttgcgcc ttgttatgta gttaatgctc agaatatgat agataaaata
     1261 gagacgcaaa acagggcatg ctcataacat gatgataaca taaaaaatta gtaacaataa
     1321 atgaaagaat catgggcgct caacaaaaa
//
LOCUS       DADLXX010000301         1345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLXX010000301 DADLXX010000000
VERSION     DADLXX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.308"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            443..1192
                     /locus_tag="JNX27_004240"
     CDS             443..1192
                     /locus_tag="JNX27_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7885171.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          376 a          274 c          279 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aattt
//
LOCUS       DADLXX010000302         1327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLXX010000302 DADLXX010000000
VERSION     DADLXX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.309"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            92..449
                     /locus_tag="JNX27_004241"
                     /pseudo
     CDS             92..449
                     /locus_tag="JNX27_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            644..883
                     /locus_tag="JNX27_004242"
     CDS             644..883
                     /locus_tag="JNX27_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7885172.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
BASE COUNT          325 a          335 c          402 g          265 t
ORIGIN      
        1 cttgccggtg ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc
       61 gcaggcgacc gcgtaaaccg tcatcaaggc aatgtgccgc gtactccggg tggcccgcag
      121 cggctggtat acgtggtgtc agcggcggac aaggataaac acacgtcagc agttccgtca
      181 gcactgcgac agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc
      241 ctgacggatg aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc
      301 tgcaccgtca ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg
      361 cacacggcct gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga
      421 accagaagtg ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg
      481 gggcgccggg ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg
      541 ctgatgaaag ccgtctgccg caggatcagt gatgcacgtt ttatgagact gctgtggaaa
      601 acgccgtgtt gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt
      661 gatctcgccg ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg
      721 ctacctgagt ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg
      781 ccgaagtact gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta
      841 tgccgatgac tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg
      901 cagcacaaga gatgagggaa ggcccctcgg tgtcggcgtg caggcgcagg caccaaacca
      961 tccactgctg ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg
     1021 agccgatcaa gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa
     1081 gcatatagat gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg
     1141 cgcggcatcc ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac
     1201 ctgtcacccc gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca
     1261 atgcggggta cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa
     1321 ttggggc
//
LOCUS       DADLXX010000303         1315 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLXX010000303 DADLXX010000000
VERSION     DADLXX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.310"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(21..824)
                     /gene="dkgB"
                     /locus_tag="JNX27_004243"
     CDS             complement(21..824)
                     /gene="dkgB"
                     /locus_tag="JNX27_004243"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7885173.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGTENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDHLVSPEGLAPEWD"
     gene            complement(987..1063)
                     /locus_tag="JNX27_004244"
     tRNA            complement(987..1063)
                     /locus_tag="JNX27_004244"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Asp,seq:gtc)
     gene            complement(1118..1233)
                     /gene="rrf"
                     /locus_tag="JNX27_004245"
     rRNA            complement(1118..1233)
                     /gene="rrf"
                     /locus_tag="JNX27_004245"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          329 a          319 c          300 g          367 t
ORIGIN      
        1 ggaggagctg tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg
       61 tggtcgttgc aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt
      121 tgtgccttaa gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa
      181 ccttccccca tagcccacgc cagaatcact tgtgccggaa tcgcattgtg tttagctgca
      241 atacgagcaa taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg
      301 atatgaatac cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag
      361 agttcaatct ggttggtagc gatgttttca gtgccaacag cagcaatcgc tttttccatc
      421 aatgggatcg tgaagttgga aataccgatc tcacgcgtca gcccttgttt tttggcttcc
      481 agcagcgcct tcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt
      541 agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctctttcag actcgggatc
      601 aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt
      661 ggcacgccac tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgcg
      721 gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg
      781 tctttcagac ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa
      841 tgctattttc gttaactgag gtgattatgg gcttcagact gttgcggaaa aagagcgtaa
      901 aacgcagagg atttttgcga ttctagcaat aatagatatc cagacgcaaa aaaacatcga
      961 gcgttaactc gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc
     1021 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa
     1081 ccaccggttt tatgaccggc ttactgctta atttgatgcc tggcagttcc ctactctcgc
     1141 atggggagac cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg
     1201 gtcaggtggg accaccgcgc tacggccgcc aggctaattc tgttttatca gaccgcttct
     1261 gcgttctgat ttaatctgta tcaggctgaa aatcttctct caatccgcca aaaca
//
LOCUS       DADLXX010000304         1296 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLXX010000304 DADLXX010000000
VERSION     DADLXX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.311"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..815)
                     /locus_tag="JNX27_004246"
     CDS             complement(<1..815)
                     /locus_tag="JNX27_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7885174.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JNX27_004247"
                     /pseudo
     CDS             907..993
                     /locus_tag="JNX27_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JNX27_004248"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JNX27_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          363 a          219 c          336 g          378 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaaatg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctt
//
LOCUS       DADLXX010000305         1278 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.312, whole genome shotgun sequence.
ACCESSION   DADLXX010000305 DADLXX010000000
VERSION     DADLXX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.312"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            343..645
                     /locus_tag="JNX27_004249"
     CDS             343..645
                     /locus_tag="JNX27_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7885175.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            636..1118
                     /locus_tag="JNX27_004250"
     CDS             636..1118
                     /locus_tag="JNX27_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7885176.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            1192..>1278
                     /locus_tag="JNX27_004251"
     CDS             1192..>1278
                     /locus_tag="JNX27_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885177.1"
                     /translation="MSRKNQRYSTEFKAEAVKTVPENQLSISE"
BASE COUNT          323 a          271 c          311 g          373 t
ORIGIN      
        1 tggcgacggc tttcagaacc ctctttcacc attctggtgt ggactttctg gtgctccagg
       61 ttgtgtgaca tgggaactca ttctggatgg ttactctgaa agctcatatt ctgccacacc
      121 ccgatttgca gccgccaggc tgccgtggtt caggcatttc attatcaaaa tcacattaaa
      181 cggccgtaat cagacatgat ttgtgcgccg acacagatca tcgtcacaat gttcaggtct
      241 ctgccattgt ttttatcagg ggatcgcttt tcccgtaagt gattctgtat agcgttctgt
      301 taccatttgc gataacatta attgcatagc aaattgttac caatggaggt ttttatgtct
      361 acagctgcaa gcgtccgtaa aacaccgcga gaacatcaga ttaacatccg ggcaactgat
      421 gaggaacgag ccgttattga ttatgctgcg agcttggtca ataagaaccg taccgatttc
      481 atcatggaac tggcgtacca ggaagctaaa aacattatcc tggatcaacg tttatttgtg
      541 ctggataacg aacgttatga ttcgtttatt acgcaactgg aagctcctgt gcagaatgca
      601 gaagggcgtg aacgtctgat ggcggtaaag cctgaatgga aataaatgtc accgcgccag
      661 cattgttgac ggatgagcat atacttcagc catttgactg tggaaatgag gtgctaagta
      721 actggttacg tggtcgggct atgaaaaacc agatgctcaa tgcttctcgc acgttcgtta
      781 tttgcttgga ggatacttta cgtattgtgg gatactactc attagctact ggttccgtca
      841 ctcatgccga gcttggtcgc agtttgcggc ataacatgcc caatcctgtc cctgttgttc
      901 tgttagggcg tctggctgtt gatgtttgta cccaggggca tggtttcggt aaatggctac
      961 taagtgatgc cattcaccgg gttgtcaatc tggctgatca ggttggcatt aaggctgtta
     1021 tggtacatgc aattgatgat aacgccagag cattttatga gcgttttggt tttgttcagt
     1081 cagttgtggc acctaatacc ttgttttata aagtctgagt ctgagtctga ggtagcctga
     1141 gtttaacgga cactccttcc tgaaatagaa tggcatcaga aggagccaat aatgagcaga
     1201 aaaaaccaac gttactctac ggagtttaaa gccgaagccg tcaaaacggt tcctgaaaat
     1261 cagctttcga tcagtgaa
//
LOCUS       DADLXX010000306         1277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLXX010000306 DADLXX010000000
VERSION     DADLXX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.313"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNX27_004252"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNX27_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7885178.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          229 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADLXX010000307         1260 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLXX010000307 DADLXX010000000
VERSION     DADLXX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.314"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..171)
                     /locus_tag="JNX27_004253"
     CDS             complement(<1..171)
                     /locus_tag="JNX27_004253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885179.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1260
                     /locus_tag="JNX27_004254"
     CDS             308..>1260
                     /locus_tag="JNX27_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7885180.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNG"
BASE COUNT          330 a          313 c          366 g          251 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
//
LOCUS       DADLXX010000308         1226 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLXX010000308 DADLXX010000000
VERSION     DADLXX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.315"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..884
                     /locus_tag="JNX27_004255"
     CDS             <1..884
                     /locus_tag="JNX27_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7885181.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYVEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1226
                     /locus_tag="JNX27_004256"
     CDS             884..>1226
                     /locus_tag="JNX27_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7885182.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          279 c          316 g          306 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgtcga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgt
//
LOCUS       DADLXX010000309         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLXX010000309 DADLXX010000000
VERSION     DADLXX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.316"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            238..1210
                     /locus_tag="JNX27_004257"
                     /pseudo
     CDS             238..1210
                     /locus_tag="JNX27_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          362 a          262 c          280 g          306 t
ORIGIN      
        1 ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca gtctgctgag gtatatgccg
       61 gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt ataaaaaaca gcctgttgcg
      121 gtataagaat cgaccatgca gagagagaaa acaccggagt ggagggaaaa gcagaaaagc
      181 tcacgtggca taaggcgtgg acagcggtat cgtctggttt ttcaatttcc tatacgtgaa
      241 cgctatatag cccgattacg caacagagtt gaaaaccgtc tctggcattc actggcagct
      301 tgcattgatg actctcagac tcaacagctt ctggatcttc tttcggttcc tgccggaagc
      361 cgctattcgt tacttgatca attacgcgcc ggacctacta aagtgaatgc cacatcactg
      421 gtacaggcta taggtcgcct tcagacgata cgtagcctag gagtgacatt acctgcaatt
      481 acgccagttt cggatatccg tattgcggcc atggcacgtt atgcctcaac ggctaagata
      541 acagcgcttc agcgcttacc agaaaaaaga aaactggcta cgcttgttgc attctcttgc
      601 tgcatggagg cgacagctca ggatgatgct cttgagttac ttgaagcgct actccgggat
      661 ctgtttaatg aagcggttca agcagataaa cgtaaccgcc aacgaacact taaagatctg
      721 gatcgtgcag cagaaatact ggcaaaagca tgtcggatgc ttcttgacga taaattatct
      781 gatactgatg ttcgtgatag tatcttcaat atcattcccg aagatgtact gactcatgca
      841 gtaaataatg tgacatcaat tattcgacca gataataacg tttacttcaa tgaacttgat
      901 tctaaattca aaacggtgcg gcgtttctta cctgacttac tttccagaat acattttgag
      961 ggcaatgcct cagccaaaac attaattgaa gcgctttgct ggattgaagt taatttaaaa
     1021 aagaaaaaaa cagataacga tgcaccacgc gaaataatca ataaaccgtg gcaacagcat
     1081 gtgataagaa aagatggtag tattgatttc cacgcctata cattttgtgc acttaaggaa
     1141 cttcagcttg cactgaaaaa acgggatatt tatgtcaatc ccagctggcg ttatgcagat
     1201 ccccgggcag
//
LOCUS       DADLXX010000310         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLXX010000310 DADLXX010000000
VERSION     DADLXX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.317"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNX27_004258"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNX27_004258"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7885183.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..>1210
                     /locus_tag="JNX27_004259"
     CDS             943..>1210
                     /locus_tag="JNX27_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885184.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSA"
BASE COUNT          280 a          327 c          297 g          306 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga
//
LOCUS       DADLXX010000311         1207 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLXX010000311 DADLXX010000000
VERSION     DADLXX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.318"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..925
                     /locus_tag="JNX27_004260"
     CDS             14..925
                     /locus_tag="JNX27_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7885185.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JNX27_004261"
     CDS             941..1168
                     /locus_tag="JNX27_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885186.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          340 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggcaa
//
LOCUS       DADLXX010000312         1185 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLXX010000312 DADLXX010000000
VERSION     DADLXX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.319"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>1185
                     /gene="amn"
                     /locus_tag="JNX27_004262"
     CDS             <1..>1185
                     /gene="amn"
                     /locus_tag="JNX27_004262"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7885187.1"
                     /translation="AIGKYITIGELPDENARKQGLFVYPSLTVTWDGSTTNPPKTRAF
                     GRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYVIDGSEL
                     TLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLARLRHYT
                     GTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITAETEAPEE
                     AISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDVWLMIGHC
                     GGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGE
                     EVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPYGT
                     LLC"
BASE COUNT          287 a          312 c          276 g          310 t
ORIGIN      
        1 gccattggca aatatattac aattggcgaa ttacctgatg aaaacgcccg taaacaaggt
       61 ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaacaaa tccccccaaa
      121 acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat tactcgccct
      181 actctctttc gttcgtatct gaatgagcaa ctaacgttgc tttatcagga ttatggtgcg
      241 catatctctg tgcaaccctc gcaacatgaa atcccttatc cttatgtcat cgatggctct
      301 gaattgacac ttgatcgctc aatgagcgct gatttaactc gctacttccc gacaacagaa
      361 ctggcgcaaa tcggcgatga aactgcagac ggcatttatc atccaacgga attctccccg
      421 ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg ccattatacc
      481 ggtacgccag tagaacattt tcaaccgttc gtcttgttta ccaactacac acgttatgtg
      541 gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc ctacattgcc
      601 ctttcttgtg ctggcgggaa ctggatcact gccgaaaccg aagcgccaga agaagccatt
      661 tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac cgctgatggt
      721 cagggtatta ctctggtgaa tattggcgtg ggaccgtcca atgctaaaac catctgcgat
      781 catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg cggattacgt
      841 gaaagccagg ccattggaga ttatgtcctt gcacacgctt atttacgcga tgaccacgtt
      901 cttgatgcgg ttctgccgcc cgatatacct attccgagca ttgctgaagt gcaacgtgcg
      961 ctttatgacg caaccaagtt agtcagcggc aggcctggtg aggaagtcaa acagcggctg
     1021 cgtacaggta ctgtggtaac cacagatgac aggaactggg agttacgtta ctcagcttct
     1081 gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc aaccattgcc
     1141 gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgt
//
LOCUS       DADLXX010000313         1169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLXX010000313 DADLXX010000000
VERSION     DADLXX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.320"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..398
                     /locus_tag="JNX27_004263"
     CDS             <1..398
                     /locus_tag="JNX27_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885188.1"
                     /translation="YLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVF
                     VAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTF
                     QLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(399..1043)
                     /locus_tag="JNX27_004264"
     CDS             complement(399..1043)
                     /locus_tag="JNX27_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7885189.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          297 a          269 c          279 g          324 t
ORIGIN      
        1 gctacctcac cctggtgctg gtggcaattc tgattatgct gccgactctc ggcatcggtg
       61 atatgaacaa tctctataaa tggctgttga atcttaactc ggtagtgatg ccgctgcgtt
      121 atttatgggt atttgttgca tttattgcag tcgttcgcct ggcgcagaaa tataaaccag
      181 aatatgtctt tattcgtaat aagcctctgg caatgaccgt cgggatttgg tgttttgcct
      241 ttaccgcctg tgcctgtttg acggggatct tcccgaaaat ggaagccttc actgcagagt
      301 ggaccttcca gttggcgctg aatgttgcaa cgccgtttgt gctggtagga ttaggactga
      361 tattcccgct gctggcgcgt aaagcgaata gcaaataatt attgtggtcg gtgacgctga
      421 ctctggcaga tctgacccgt ttttacataa tcgagaaatg cgcgcaagcc gcaggacata
      481 tactggcggt ttgggtaata gacttggaag cctggacgct ctgtgatcca gtcttccagt
      541 acacattcta aacgaccagt atcaaggtac tccttgatct gttcatagag cagatacccg
      601 ataccagcac ccatcaaaac ggcctccagt tcagcatcaa catcatccag aatgatattt
      661 cctgcgacag caatttccag ctcctttgca aattgccagt ggaaaggttt gccgctggga
      721 taacgaaaaa caacacattg atgattaagc agatcatgag gatggcgggg tttgccatat
      781 cttgcaaagt actctggcgt tgctgctaca cataatttca ctggtgggcc gatcgccacg
      841 ctgatcatat ctttttcgac gatgccacta agtcgaacgc cagcgtcaaa gccctgttgg
      901 acgatatcga taagagaatc gtcagttgtg agctctactt taatatcagg atattcacgt
      961 gtgaaaccaa ccagtaaagg cattaaaaat atacgtgcag ctacccgggc ggcattgatt
     1021 ttgagcgttc ctgtcggtgt cagacgaaaa tcattcatct catctaacat gatctgaatt
     1081 tcgtcgaaag ctgggcgcaa tcgttcatag agattagaac ccgcttccgt taacgatacg
     1141 cttcgggttg tccgattgaa taagcgaat
//
LOCUS       DADLXX010000314         1154 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLXX010000314 DADLXX010000000
VERSION     DADLXX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.321"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(569..781)
                     /locus_tag="JNX27_004265"
     CDS             complement(569..781)
                     /locus_tag="JNX27_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885190.1"
                     /translation="MVVWRFGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINL
                     LDKSDYVLAHGLNTDDQLAVGIVYQF"
     gene            841..>1154
                     /locus_tag="JNX27_004266"
     CDS             841..>1154
                     /locus_tag="JNX27_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7885191.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGR"
BASE COUNT          318 a          213 c          298 g          325 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc cccgaatctc cagacaacca
      781 atatcactta tttaagtgat agtcttaata ctagttttta gactagtcat tggagagcag
      841 atgattgatg tcttaggacc ggagaaacgc agacggcgta ctacacagga aaagatcgct
      901 attgttcagc agagttttga accgggaatg acggtctccc ttgttgcccg gcaacacggt
      961 gtggcagcca gccagttatt tctctggcgc aagcaatacc aggagggaag tcttactgct
     1021 gtggccgcag gagaacaggt cgttcctgcc tctgaacttg ctgctgccat gaagcagatt
     1081 aaagaactcc agcgcctgct cggcaaaaaa acgatggaaa atgaactcct taaagaagcc
     1141 gttgaatatg ggcg
//
LOCUS       DADLXX010000315         1151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLXX010000315 DADLXX010000000
VERSION     DADLXX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.322"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..233)
                     /locus_tag="JNX27_004267"
     CDS             complement(<1..233)
                     /locus_tag="JNX27_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7885192.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQ"
     gene            complement(500..1006)
                     /locus_tag="JNX27_004268"
     CDS             complement(500..1006)
                     /locus_tag="JNX27_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885193.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQSDLSQGDQLTEMLSFLTSEEREAL
                     TNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEASILS
                     ESVNAKAG"
BASE COUNT          296 a          265 c          242 g          348 t
ORIGIN      
        1 tgttggctgg ctgcaaccgc taatgggctg gaaccgaggt tgccgatttg cacgtcgcca
       61 gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc cacgctcgcc
      121 ccgctctctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaggtt
      181 tgatacgcca cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca
      241 agaagtgtgt tatatgacta ggtaatttat tcaagataac cgggaatggg taaaaaaaga
      301 cgggatttga tgggattcaa taagttatgc gccgattgca ttcaagataa aacgagttaa
      361 aaatgctcaa tgcttcgata tcgataaaac attcaagata ggcgcgccgt gtattttaat
      421 tcttttaaga ctcttcaaaa atgtcatcgt agcctttaaa aacgaatttt aaaggcaccc
      481 taaaggcagg tcaatgaagt taacccgcct tcgcgtttac actctctgag agaatactag
      541 cttcaatctc ttccaaaatc tctctgactt gctcgtcggg catatcaacg agcatcaacg
      601 ctgccaggcg tttacggccc tctagttgca gtagctcttg agtttcctta tctaacaagc
      661 gtagcacagt ctcaacacct ttccttgcga gcatgtttgt aagggcttct cgttcctcac
      721 tggtaagaaa cgacaacatt tctgttagct gatccccttg gcttaaatca ctttgtgttt
      781 ttggtggatt cccgttaccg gtttttagcc attcaagact aacaccttca ctttctgcaa
      841 tgcgtaccaa tatgctttct cgaggttctg gtgtatttcc ctttttaaaa tagctttgta
      901 acgtattgat atttatgcca caggctctgg cggcagcact tcttgatgga taccgcctaa
      961 taagataagc catgcgctcc acgataggtt cttttgcctg ctccataaaa gtacctctag
     1021 gtatttttct gtataaaggt tcttttccat ctatctaatt gaatggaaag gtaattttaa
     1081 aaatccaaat aaaatacaaa aaaatacctt gtattggtgc tttttgtgat ctgcgatttt
     1141 atttactcgg a
//
LOCUS       DADLXX010000316         1126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLXX010000316 DADLXX010000000
VERSION     DADLXX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.323"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            252..1007
                     /gene="mlaA"
                     /locus_tag="JNX27_004269"
     CDS             252..1007
                     /gene="mlaA"
                     /locus_tag="JNX27_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7885194.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          276 a          260 c          293 g          297 t
ORIGIN      
        1 gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggt tgcgtttgcc
       61 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
      121 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
      181 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
      241 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
      301 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
      361 gcaccatgta caacttcaac ttcaacgtat tagacccgta tattgttcga ccggtcgctg
      421 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg tttgagcaac tttactggca
      481 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
      541 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
      601 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
      661 ttggtcatta tggcgtgggt tatgggcctt acgttcagtt accgttctac ggtagcttca
      721 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
      781 cctggccgat gtctgtgggt aaatggacgc ttgaagggat cgaaacccgc gctcagctgc
      841 tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
      901 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
      961 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     1021 aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac aagtaaaagg
     1081 agataaaccg acaatgaccc ggacgtaatg atgtaaaaat actgat
//
LOCUS       DADLXX010000317         1125 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLXX010000317 DADLXX010000000
VERSION     DADLXX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.324"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..89)
                     /locus_tag="JNX27_004270"
     CDS             complement(<1..89)
                     /locus_tag="JNX27_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein hokD"
                     /protein_id="HAY7885195.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIAL"
     gene            complement(292..510)
                     /locus_tag="JNX27_004271"
     CDS             complement(292..510)
                     /locus_tag="JNX27_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885196.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(684..1019)
                     /locus_tag="JNX27_004272"
     CDS             complement(684..1019)
                     /locus_tag="JNX27_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885197.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
BASE COUNT          293 a          282 c          200 g          350 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta gttgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc gccctggtaa tgctg
//
LOCUS       DADLXX010000318         1120 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLXX010000318 DADLXX010000000
VERSION     DADLXX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.325"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..1120
                     /gene="pdeF"
                     /locus_tag="JNX27_004273"
                     /pseudo
     CDS             1..1120
                     /gene="pdeF"
                     /locus_tag="JNX27_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT          245 a          254 c          250 g          371 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt ggggcttccg ctgttcctgc cttctttaat
       61 cttgcccatt ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct
      121 cttttatctg cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt
      181 gccagggatc accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac
      241 gctctcatta acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt
      301 ctttactccc cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat
      361 attctggcag atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt
      421 tgtaggatta ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac
      481 tttaatcaat tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt
      541 catcattcgg gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca
      601 gcaggttgat gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc
      661 tttattgttg ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta
      721 taccttgtca ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct
      781 gatttcgctg ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat
      841 tcccatttat cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt
      901 ctcttttatt gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg
      961 cattcagcgg cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa
     1021 tcgcgcgttg cgtgatgccc tctggtctgc gctttgttat ttacgcatcc ctggcatgga
     1081 aatgctggtt aagaactatg gcatcatgct gcggattcaa
//
LOCUS       DADLXX010000319         1111 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLXX010000319 DADLXX010000000
VERSION     DADLXX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.326"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..1019
                     /locus_tag="JNX27_004274"
                     /pseudo
     CDS             1..1019
                     /locus_tag="JNX27_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1111
                     /locus_tag="JNX27_004275"
     CDS             992..>1111
                     /locus_tag="JNX27_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY7885198.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVS"
BASE COUNT          316 a          271 c          255 g          269 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctagacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcatt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag c
//
LOCUS       DADLXX010000320         1110 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLXX010000320 DADLXX010000000
VERSION     DADLXX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.327"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..332
                     /locus_tag="JNX27_004276"
     CDS             <1..332
                     /locus_tag="JNX27_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885199.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
     gene            332..679
                     /gene="tnpB"
                     /locus_tag="JNX27_004277"
     CDS             332..679
                     /gene="tnpB"
                     /locus_tag="JNX27_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7885200.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            699..>1110
                     /locus_tag="JNX27_004278"
     CDS             699..>1110
                     /locus_tag="JNX27_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7885201.1"
                     /translation="MDTSLSHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGE"
BASE COUNT          266 a          314 c          301 g          229 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag cagataaata caccgtccga cagtctgtgc tgtgagctgg
      241 ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta ttacagatac
      301 ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag gttcgcgtat
      361 ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg cctcaaaagt
      421 tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc gcggacgccg
      481 gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct ttaccagacg
      541 cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc accttactcc
      601 ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac gaacggaacg
      661 cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca ctttctcatg
      721 agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc cagatggcga
      781 aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc aaccggctga
      841 aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa aaacttcgcg
      901 caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt caggaagaaa
      961 tggcggaaac gctgggtgag caatatgacc cggtactgcc atctcccctg cgccagtctt
     1021 cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc cgtccggaag
     1081 aggaatgctg cccggcctgt ggtggcgaac
//
LOCUS       DADLXX010000321         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLXX010000321 DADLXX010000000
VERSION     DADLXX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.328"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..387)
                     /locus_tag="JNX27_004279"
     CDS             complement(<1..387)
                     /locus_tag="JNX27_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7885202.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JNX27_004280"
     CDS             816..1097
                     /locus_tag="JNX27_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885203.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          307 a          249 c          252 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADLXX010000322         1087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLXX010000322 DADLXX010000000
VERSION     DADLXX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.329"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            256..331
                     /locus_tag="JNX27_004281"
     tRNA            256..331
                     /locus_tag="JNX27_004281"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Ile,seq:cat)
     gene            333..409
                     /locus_tag="JNX27_004282"
     tRNA            333..409
                     /locus_tag="JNX27_004282"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:367..369,aa:Lys,seq:ttt)
     gene            412..487
                     /locus_tag="JNX27_004283"
     tRNA            412..487
                     /locus_tag="JNX27_004283"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:445..447,aa:Thr,seq:tgt)
     gene            491..565
                     /locus_tag="JNX27_004284"
     tRNA            491..565
                     /locus_tag="JNX27_004284"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:524..526,aa:Gly,seq:tcc)
     gene            858..1073
                     /locus_tag="JNX27_004285"
     CDS             858..1073
                     /locus_tag="JNX27_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7885204.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          268 a          237 c          273 g          309 t
ORIGIN      
        1 atgagaacac gttcaaaaaa aagacggcat tttcaaagca aagtgttgca aaagtagatt
       61 gaaaggcata tattcatgca aatctgatat tttgccgatt ttgtacgtga tggcaaaagc
      121 aaacaaaacc cgcccacaag cgggtttttt gtgccactta tctcggatag acatggtgaa
      181 tgcgctggtg gaggagataa gggtgatttt tgaatgcttg caacattgat ttcgtaacgt
      241 tattatcctg cgctcggccc tttagctcag tggtgagagc gagcgactca taatcgccag
      301 gtcgctggtt caaatccagc aagggccacc aaccgccact agctcatcag gaaagagcgt
      361 caacccttta agttgagtgt gcgaggttcg agtccccggt ggcggtccag tgccgacttg
      421 gctcagtagg tagagcaact gacttgtaat cagtaggtca ccagttcgat tccggtagtc
      481 ggcaccatat gcgggcatcg tataatggct attacctcag ccttccaagc tgatgatgcg
      541 ggttcgattc ccgctgcccg ctccagttag agtctttcag tctgcgatga tgggaaatcc
      601 cggagtgact gaaagacgtt taagttatga atgatcgctt ttttttgcaa aattgctgtg
      661 cagaaatact aaccttcggg caggcgatca ttcataagca ctctgctttt attccgatta
      721 actgtgagtg gtttgttgga tagagtgctt tccttactgt atatatcgtt tcgcccgctt
      781 ttgcggtttt ttcttttcaa atccctttca tttctcagtg taaaactacg ccatccgtta
      841 tttgcggagg tgaggctatg aaatccatgg acaaaatttc aacgggcatt gcctacggca
      901 cctccgcagg cagtgctggc tactggtttt tacagtggct tgatcaggtc agtccgtcac
      961 agtgggctgc gattggtgta ctggggagtc tggttctggg cttcctgact tatctgacaa
     1021 atctgtactt caaaatcaga gaagacaagc gtaaggctgc gagaggtgaa taatgtcgcc
     1081 atcatta
//
LOCUS       DADLXX010000323         1078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLXX010000323 DADLXX010000000
VERSION     DADLXX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.330"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..276
                     /locus_tag="JNX27_004286"
     CDS             <1..276
                     /locus_tag="JNX27_004286"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7885205.1"
                     /translation="KKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSK
                     QQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            273..>1078
                     /locus_tag="JNX27_004287"
     CDS             273..>1078
                     /locus_tag="JNX27_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HAY7885206.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
                     GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSM
                     SAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWR"
BASE COUNT          267 a          280 c          306 g          225 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      481 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
      541 gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac
      601 acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc
      661 agaagtggcc aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag
      721 tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg
      781 cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg
      841 ttatcgttca cacggaccgt ggaggccagt actgttcagc agattatcag gcgcaactga
      901 aacggcataa tctgcgtgga agtatgagcg caaaaggttg ctgctacgat aatgcctgcg
      961 tggaaagctt ctttcattcg ctgaaagtgg aatgtatcca tggagaacac tttatcagcc
     1021 gggaaataat gcgggcaacg gtgtttaatt atatcgaatg tgattacaat cggtggcg
//
LOCUS       DADLXX010000324         1072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLXX010000324 DADLXX010000000
VERSION     DADLXX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.331"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..87)
                     /gene="rrf"
                     /locus_tag="JNX27_004288"
     rRNA            complement(<1..87)
                     /gene="rrf"
                     /locus_tag="JNX27_004288"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(133..>1072)
                     /locus_tag="JNX27_004289"
     rRNA            complement(133..>1072)
                     /locus_tag="JNX27_004289"
                     /product="23S ribosomal RNA"
BASE COUNT          244 a          328 c          232 g          268 t
ORIGIN      
        1 aaggtcccaa ctaccatcgg cgctagagag cttaacttcc gtgttcggta tgggaacggg
       61 tgtgacctct ctgccatcat caccaaacta tgaaggcata ttccttcaaa actagataac
      121 atttgctaca tattatatgg ttaagtcctc gatctattag tattcgtcag ctccacatgt
      181 caccatgctt ccacctcgaa cctatcaacc tgatcatctt tcagggatct tactagctta
      241 cgctatggga aatctcatct tgaggggggc ttcatgctta gatgctttca gcacttatcc
      301 cttccgcaca tagctaccca gctatgccct tggcagaaca actggtacac cagcggtgcg
      361 tccatcccgg tcctctcgta ctaaggacag ctcctctcaa atttcctacg cccacgacgg
      421 atagggaccg aactgtctca cgacgttctg aacccagctc gcgtaccgct ttaatgggcg
      481 aacagcccaa cccttgggac cgactacagc cccaggatgc gatgagccga catcgaggtg
      541 ccaaacctcc ccgtcgatgt ggactcttgg gggagataag cctgttatcc ccggggtagc
      601 ttttatccgt tgagcgatgg cccttccatg cggaaccacc ggatcactaa gcccgacttt
      661 cgtccctgct cgacttgtag gtctcgcagt caagctccct tatgcctttg cactctacga
      721 atgatttcca accattctga gggaaccttt gggcgcctcc gttacacttt aggaggcgac
      781 cgccccagtc aaactgccca cctgacactg tctcccgggt cgataagacc cgtaggttag
      841 aatttcaata cagtcagggc ggtatcccac cagcgcctcc accgaagcta gcgctccggt
      901 ttcaatggct cccgcctatc ctgtacaaac tgtaccaaaa ttcaatatca ggctacagta
      961 aagctccacg gggtctttcc gtcctgtcgc gggtaacctg catcttcaca ggtactataa
     1021 tttcaccgag tctctggttg agacagtgcc caaatcgtta cacctttcgt gc
//
LOCUS       DADLXX010000325         1071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLXX010000325 DADLXX010000000
VERSION     DADLXX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.332"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..767
                     /locus_tag="JNX27_004290"
     CDS             <1..767
                     /locus_tag="JNX27_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7885207.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..1069
                     /locus_tag="JNX27_004291"
     CDS             788..1069
                     /locus_tag="JNX27_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7885208.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     CWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQSVNRPGNPGD"
BASE COUNT          346 a          239 c          244 g          242 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga atttattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacgat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaat gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaacatt
     1021 actgacttac ctgcaaagcg tgaaccgccc cgggaatcct ggagactaaa c
//
LOCUS       DADLXX010000326         1071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLXX010000326 DADLXX010000000
VERSION     DADLXX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.333"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..269)
                     /locus_tag="JNX27_004292"
     CDS             complement(<1..269)
                     /locus_tag="JNX27_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885209.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNAQVIRAAGGAGALSDWLLRH"
     gene            complement(271..549)
                     /locus_tag="JNX27_004293"
     CDS             complement(271..549)
                     /locus_tag="JNX27_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885210.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(616..866)
                     /locus_tag="JNX27_004294"
                     /pseudo
     CDS             complement(616..866)
                     /locus_tag="JNX27_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          273 a          277 c          263 g          258 t
ORIGIN      
        1 atatggcgca acagccagtc agacaatgca ccagcaccac cagcagcacg aatcacctgt
       61 gcgttactga aaaacggcag caatgttttg tcttccacca gcggctggcg aacggcagga
      121 acgaccccgg acggcagatt acgcatgctt ttcggttccg gctccaccag taaccgggta
      181 ttgtggaata ccggcatgga ttcacgaccg ggcttaagga ccaccagccc gagttccgga
      241 accagaacag gtcgaagtaa tacccgcacg ttacctccag atccgttgct ggaatgtgcg
      301 ggacggacgc ggtggctgtt cggagtaagg gagcctgacg gagattatcc agtgacggta
      361 gtcgaggcta agggcttttt taacctcgca tccgcgcctg cggtaacact gaatgagcca
      421 ttcggcctgt tcttcagtgc atgagggatg ctggtaccag tctgacttaa atgcgtgaga
      481 ataccgctcg tgcgtgtggg caagaacggt cgaattatca tgattgtaat attttgcgtt
      541 gcgtgccatc ggttttctcc ggtggcacgg tgttactcag cgggagttca gccccgcgca
      601 agattgtaga tgagtttatt cttctgaaaa agcagaaaag ccagctttta ttccggtctc
      661 tttcaatgcc tgtaatgaag tgacaaactc accgtcgcgc aagataaatc cgtccgtcac
      721 tcgggcatcc acaaaattaa ttaacgcagc cccatttttt gcaaacacac aatgcggtaa
      781 tgactaacaa tatttccatt ttcaacgcac acagcataga ggccatcttc acaaaaaatt
      841 ttacgcagtt cttcgatgtt catcatcaga atccttccag ataagtagca ctcccctgtt
      901 cggggtccat cccttttatc cctgcgcgct acttaagtat ttttgattct attccggcac
      961 catccagaac ttcaaatgcg ttgaaaataa aaacaaaaac ccgccgaagc gggttaagtg
     1021 cgggtgcgtt gaggatgcct gccacatcag aggtggcgag ggatttctca c
//
LOCUS       DADLXX010000327         1050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLXX010000327 DADLXX010000000
VERSION     DADLXX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.334"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            508..1050
                     /locus_tag="JNX27_004295"
                     /pseudo
     CDS             508..1050
                     /locus_tag="JNX27_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          376 a          190 c          187 g          297 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa atgtattacg cgtaacaaac tcatcttctt ctggcataag tgaaaaacat
      661 ttagatcact gtgctaatac tgtgaaaaac tttttaagga agtctatagc agcgcaaagt
      721 tatagcaaaa tgttttctca agggaccagt ttcaaatcac taaatctttc tattgaagcg
      781 ccatctggag cacggtcatc atttcggtca cttgagcatc tcgataaggt gtccagacac
      841 tatatctctg agataataca aaaagttcat cctctttctt cagacgaacg tcacttactt
      901 tctattatta ttaattctaa ttttaacttc agacatcaaa gtaactctaa tctgtcaaat
      961 aatattctta atattaatat cctttgataa aattcagtct gaaaatatac aaacacataa
     1021 gaatacatac tctgaggata ttaaagaaat
//
LOCUS       DADLXX010000328         1048 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLXX010000328 DADLXX010000000
VERSION     DADLXX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.335"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(239..993)
                     /locus_tag="JNX27_004296"
                     /pseudo
     CDS             complement(239..993)
                     /locus_tag="JNX27_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          277 a          245 c          244 g          282 t
ORIGIN      
        1 ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca gtctgctgag gtatatgccg
       61 gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt ataaaaaaca gcctgttgcg
      121 gtataagaat cgaccatgca gagagagaaa acaccggagt ggagggaaaa gcagaaaagc
      181 tcacgtggca taaggcgtgg acagcggtat cgtctggttt ttcaatttct tatacgtgaa
      241 cagctttatg gttaatctgt ttcccttctc gatgaagaga cagcgttacc ctacggtatc
      301 cgtatcggcc tctattctca tgataaatct caccaatacg ctttttaacg tccgcatact
      361 tgtcaggctt gctgagagcc tttagatggt aataaaacgt actgcgcggt atctccgcag
      421 ccctgagaag ctcatcaaga ggataaaact gccttagctc gttgagtact ttcacttttt
      481 cgtgggatga gctaaggctt tcagcttttt tagatacaca agccgcgttt caagaaatcg
      541 aacttgcctt tcaagatcct caatgcgtcg gtcttttgac agctccaatg ctgatgccgc
      601 tttttctgga tcaactgata ttgcaatgtt tcttttggtg ccaatcttga gcgcgcgtaa
      661 accagcttct ccgcgctctt cataaacctt cagccacctg gctacagaac cactaccagc
      721 aagcataaag tgagcagcag cctgattaag ggacatgtgc tgctcaatca cagctttcac
      781 gaccttaata cgcaactctg gatcagcact aacaccttta ggtttgggaa ttaagccttt
      841 ttctccatgt ttttcataga gggcaaccca tgtcctgacc tgggttcggg ggacaccaaa
      901 acgtgccgag atgatcctgt aaccatcatc agtagtgaag tagtgattca cgacttcaag
      961 gcgcttttca aaagggtatt ttggctttga catatcaggg gctattccat ttcatcgtcc
     1021 aacaaaatgg gtgcagtaca taagcgca
//
LOCUS       DADLXX010000329         1026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLXX010000329 DADLXX010000000
VERSION     DADLXX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.336"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>1026)
                     /locus_tag="JNX27_004297"
     CDS             complement(<1..>1026)
                     /locus_tag="JNX27_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7885211.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFLT"
BASE COUNT          303 a          268 c          220 g          235 t
ORIGIN      
        1 gtcagaaaat agccattaac gggcgtacca gaagcgttgg tacgacataa actggctgga
       61 atatatttgc tgtcagcatc acctaatagc actttaagcg gggcatcaat cgagaatacc
      121 agcgcagcta cttgtcccag ggtattcgca atggcgtaaa tcaccattaa agtattaccc
      181 atgttgtaat actcgcccag cttctgaaag gcgtaatatt gaccgttcgt cattaagtca
      241 tccgggatat tacgcgagtc aaacatcatc cccatcgcca gtgagcccag aatggcacaa
      301 accgcaacca tcaccgccag gcatagcatc ccttttggaa attcttttcc tgggttgcgc
      361 gtttgattaa cgtaaggaga aatcttctct gctccgccaa ccgcgaaaac cagcattgaa
      421 atagtggtaa tgtaggtaaa gtcgatatga ggaatgaacg tttcccaggt aatgtttgtg
      481 gtcgcaatat gcacttcagt aatcgcaggc gcggttaccg ccatcgccac atacaggagc
      541 gacataacaa acatcgcaat ccctgccaca gaaccgacga tttttagcga tttcataccg
      601 cgtgaagcaa cccacataaa gaagataaac agcaccagcg ttaacccctg taacgctacg
      661 actgaatatt ctttgattag cgaaccgtcg cctttcatcg cccaaccgag cgcaatcaga
      721 attgcctggg gtttttgtgc cagataggga atatgcacca cccagtaggt ccacgcggcg
      781 agataagcca gtccgggtcc catcgtgtgt ttaatccagg tactgacccc gcccttccca
      841 tctttgaagg tcgagcctaa ctgaccaaca attagcgcat aaggtgtgaa atagagtgca
      901 aagataaaca cccatgaaaa aacaaccacc agcccctggt tggcatagtt attaacaacg
      961 ttgccaaaac cccagacagt gataaaagac atcagcgcaa tgttgtacca tcgcaactgt
     1021 ttttcc
//
LOCUS       DADLXX010000330         1022 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLXX010000330 DADLXX010000000
VERSION     DADLXX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.337"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(115..984)
                     /locus_tag="JNX27_004298"
     CDS             complement(115..984)
                     /locus_tag="JNX27_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY7885212.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          259 a          301 c          216 g          246 t
ORIGIN      
        1 caaaacgata gtacgcattt ctgaatggaa tattcagtgc cataattacc tgcctgtcgt
       61 taaaaaattc acgtcctatt tagagataag agcgacttcg ccgtttactt ctcactattc
      121 cagttcttgt cgacatggca gcgctgtcat tgcccctttc gccgttactg caagcgctcc
      181 gcaacgttga gcgagatcga taattcgtcg catttctctc tcatctgtag ataatcccgt
      241 agaggacaga cctgtgagta acccggcaac gaacgcatct cccgcccccg tgctatcgac
      301 acaattcaca gacattccag caaaatggtg aacttgtcct cgataacaga ccaccacccc
      361 ttctgcacct ttagtcacca acagcatggc gatctcatac tcttttgcca gggcgcatat
      421 atcctgatcg ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac
      481 gacatccgcc agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca
      541 tagatcttca cgaatattag gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt
      601 catcgcagta aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg
      661 taaccattcg ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact
      721 ggggcggacc ataaacgtaa atgaacgttc cccttgatcg ttcagatcga caagcaccgt
      781 ggatgtccgg tgccattcat cttgcttcag atacgtgata tcgactccct cagttagcag
      841 cgttctttgc attaacgccc caaaaggatc atcaccgacc cgacctataa acccacttat
      901 tccgcctaat ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggcag
      961 taggcgcccg tctgattctg gcaagagatc tacgaccgca tcccctaaaa cccatacttt
     1021 gg
//
LOCUS       DADLXX010000331         1016 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLXX010000331 DADLXX010000000
VERSION     DADLXX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.338"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..273
                     /locus_tag="JNX27_004299"
     CDS             <1..273
                     /locus_tag="JNX27_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7885213.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            275..>1016
                     /locus_tag="JNX27_004300"
     CDS             275..>1016
                     /locus_tag="JNX27_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885214.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          186 c          200 g          307 t
ORIGIN      
        1 atccgtagaa atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg
       61 ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc
      121 ccttatagcc atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat
      181 atcaatgatt ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct
      241 tgtaatgttt ccgtagccaa acgagataaa taatatgcgc tacttattaa ttgtaattat
      301 atttattata ggttttagtg cattacctgt atgggcaatg gactgctatg ctgaacatga
      361 agagggaaat accgtagtca ttggttacgt accaagaatt tctatcccca gtaatggtaa
      421 aaaaggtgat aagatctggc aaagcagtga gtattttatg aatgtcttct gtaataatgc
      481 attacctgcc ccatcacctg gagaagaata cccatcagcc tggactaata taatgatgtt
      541 tttaccaggc ggtcaggact tttataatca gaactcctat atttttggcg taacatacaa
      601 tggagttgat tatgattcga ctgcccctaa tgctatcgca gcaccagaat gcattgatat
      661 aaaaggagcg ggtaccttta ataatcacta taaaaacccc gctgtctgta gtggaggtcc
      721 tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa ctctatatta aattagcaaa
      781 aaatgccaat agagttaata aaaacctggt gttacctgat gaatatatcg ctccagagtt
      841 taaaggtatg agtggcgcag gagctataga agtagacaaa aatttgacct tcaggattcg
      901 tggattaaat aatattcatg tccttgactg ctttgttaat gtcgcactgg aaccagctga
      961 tggcgttgtt gactttggta aaataaattc ccgaacaatt aaaaatacca ccgtta
//
LOCUS       DADLXX010000332         1007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLXX010000332 DADLXX010000000
VERSION     DADLXX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.339"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..960)
                     /locus_tag="JNX27_004301"
     rRNA            complement(<1..960)
                     /locus_tag="JNX27_004301"
                     /product="16S ribosomal RNA"
BASE COUNT          210 a          313 c          214 g          270 t
ORIGIN      
        1 tgctccaccg cttgtgcggg cccccgtcaa ttcctttgag tttcagcctt gcggccgtac
       61 tccccaggcg gagtgcttaa tgcgttaact tcagcactaa agggcggaaa ccctctaaca
      121 cttagcactc atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac
      181 gctttcgcgc ctcagtgtca gttacagacc agaaagtcgc cttcgccact ggtgttcctc
      241 catatctcta cgcatttcac cgctacacat ggaattccac tttcctcttc tgcactcaag
      301 tctcccagtt tccaatgacc ctccacggtt gagccgtggg ctttcacatc agacttaaga
      361 aaccacctgc gcgcgcttta cgcccaataa ttccggataa cgcttgccac ctacgtatta
      421 ccgcggctgc tggcacgtag ttagccgtgg ctttctggtt aggtaccgtc aaggtgccag
      481 cttattcaac tagcacttgt tcttccctaa caacagagtt ttacgacccg aaagccttca
      541 tcactcacgc ggcgttgctc cgtcagactt tcgtccattg cggaagattc cctactgctg
      601 cctcccgtag gagtctgggc cgtgtctcag tcccagtgtg gccgatcacc ctctcaggtc
      661 ggctacgcat cgttgccttg gtgagccgtt acctcaccaa ctagctaatg cgacgcgggt
      721 ccatccataa gtgacagccg aagccgcctt tcaatttcga accatgcggt tcaaaatgtt
      781 atccggtatt agccccggtt tcccggagtt atcccagtct tatgggcagg ttacccacgt
      841 gttactcacc cgtccgccgc taacttcata agagcaagct cttaatccat tcgctcgact
      901 tgcatgtatt aggcacgccg ccagcgttca tcctgagcca ggatcaaact ctccaataaa
      961 gttagtttgt ctagcatcta aaataaaaat tgacgtttca cgttgtt
//
LOCUS       DADLXX010000333          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLXX010000333 DADLXX010000000
VERSION     DADLXX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.340"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..71)
                     /gene="ospE1"
                     /locus_tag="JNX27_004302"
     CDS             complement(<1..71)
                     /gene="ospE1"
                     /locus_tag="JNX27_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY7885215.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNP"
     gene            complement(253..654)
                     /locus_tag="JNX27_004303"
                     /pseudo
     CDS             complement(253..654)
                     /locus_tag="JNX27_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          255 a          212 c          209 g          307 t
ORIGIN      
        1 ggatttgata cttcaaggat gatattttct tgtttttgtg gaaggcaagg gaatatagtt
       61 tgtgtaagca tattgcttcc ttatgttata ttttagtaaa gaatttattt tttacattat
      121 agtcggctaa atgaagttta ctgtaaaaaa acgatacttt tttcgtaagc gatgagatgt
      181 ggagccagaa aaatggctgc gttacgtcat tgaactgtcc ttgacccacg atattcagac
      241 agtcgttgta tcttaagttg acattacccg ctgacgtcga tattcccttg gtgagagata
      301 acccaacgca ctatgcggat ggttttcatt gtaatgctcg atcgccactg caaggttatg
      361 caatgccgtt cttggtttcg gcatgaacgc gatgcagtct tccttcatcg ttttcacgaa
      421 ccgttctgct attccattac tctgagggct actgactgcc gtcgtgcagg gctccagatt
      481 caactctctg gcgaactgcc gtgtttcatg agccgtataa gccgaaccgt tatcggccac
      541 tggattggcc gttccggtcc tttatcgcca tatcgctcct gacatgacat cctgcaccgt
      601 tgctttgtca taatctcccg tgcctgctgc ccagtctatg gcttcacgat cgcaatatgg
      661 aacgtaacgc tgtcatcggg agaatcgtca atgcggcatt cggttaacgc ttaccaatat
      721 caacaccaac cggatttgtg gagcctccag tctgaagggg ctttatgcgt tccgtatagg
      781 atattatgat gctggagttt tgcgaagctc cactcctttc cgaacattta cacagatatt
      841 gcctaagaaa agtgaaacca taatatggtt atagtccctt tcagtgcaaa tacttagctt
      901 gttgcacaga gaaatagaag ctgcataagc tctttcttca aaaaatgtaa ataaagttaa
      961 atataggaaa aattacttaa tct
//
LOCUS       DADLXX010000334          977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLXX010000334 DADLXX010000000
VERSION     DADLXX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.341"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            87..977
                     /locus_tag="JNX27_004304"
                     /pseudo
     CDS             87..977
                     /locus_tag="JNX27_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT          258 a          229 c          242 g          248 t
ORIGIN      
        1 gttgtttact gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt
       61 atttctgagc gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt
      121 atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa
      181 tttcaccgaa gggggtcatc cagttccagt accgctatcg ctggcatggc aagcctaatc
      241 gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg
      301 acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg
      361 agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa
      421 ctctcagaga aaaggcctag gcactttatc gatcaacggt tatgaagcgc atgcatgacg
      481 ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg
      541 aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa
      601 ttagttggtg catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa
      661 gggacttcgg ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta
      721 agatttggct tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga
      781 tgcttatgct gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg
      841 acctgctgga caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc
      901 gccgtccaat cttcgaacaa attaagcctt ttctcgaaaa ggccatgaca atgtacaatg
      961 atgttctttt ccctgga
//
LOCUS       DADLXX010000335          929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLXX010000335 DADLXX010000000
VERSION     DADLXX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.342"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            373..>929
                     /locus_tag="JNX27_004305"
     CDS             373..>929
                     /locus_tag="JNX27_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7885216.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          331 a          179 c          151 g          268 t
ORIGIN      
        1 gtttaagctc cattttcaaa aaaatatatc agggtttgtt actaataact tctgaaaaca
       61 gaaaaaaata gagcagtaat tctatttcag agaggagtaa ctccatctat cctcgatacg
      121 tactgaacgt tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga
      181 gagacgcaat gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc
      241 agactccttc ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca
      301 aactgtttta aaattaataa gccatattct atttcactag aattcttatc gttgataaga
      361 attctggtta taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat
      421 caaataaaca atatatactc gttaactcca ttatctttac ctatggaact gactccatct
      481 tgtaatgaat tttatttaaa agtctggagc gaatgggaaa agaacggtac cccaggcgag
      541 caacgcaata tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta
      601 aatttatctg agttagattt aaaaacatta ccagatttac cgcctcagat aacaacactg
      661 gaaataagaa aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata
      721 catgctcaat ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt
      781 aatgcgggtg ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta
      841 cgggttcata ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg
      901 gtagtttctg gaaacagatt agacagcat
//
LOCUS       DADLXX010000336          916 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLXX010000336 DADLXX010000000
VERSION     DADLXX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.343"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>916
                     /locus_tag="JNX27_004306"
     CDS             <1..>916
                     /locus_tag="JNX27_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY7885217.1"
                     /translation="DIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRML
                     FGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIA
                     SKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAF
                     KVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEI
                     YARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKT
                     KTGRLWVYVRDDRNAGSSLPAAVWFAYSA"
BASE COUNT          249 a          231 c          246 g          190 t
ORIGIN      
        1 tgatattgag ctgcttaaag ccatgttgcg taagcaacag agtcggcttc gacagtatgc
       61 ctgtcaggtc gcgggctatg agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt
      121 gcgtcgtatg ttgttcggcc agagttcaga gaaaaagcgt cataagcttg aaaatcagat
      181 ccgacaggca gaaaaacgac tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct
      241 tctggaagat gcatcgtcag tcacagattc acctgacacc agtcccccgt cagaaaaccc
      301 gatcgccagt aagcctgaat ccccgggacg aaaatcttca cgaaaaccgc tgccggcaga
      361 acttccccgg gagacacatc gccttctgcc tgctgaaacc agttgcccgg cctgtggagg
      421 tgttctgaaa gaaatggggg aaacaatctc agagcaactg gatatcatta ataccgcctt
      481 taaagttatc gaaaccatac gtcccaaact ggcctgtagc cggtgtgatg tcatcgttca
      541 ggcaccactt ccccctaaac cgatcgaacg cggttatgcc agtgcagggt tacttgcacg
      601 gatcctggtc agcaaatata tggaacatat ccctttatat cgccagtcag aaatatacgc
      661 gcgacagggc gtggagctga gccgtaatac catggtgcgc tgggtatcag aaatggcaga
      721 caaactccgt cctctgtata tagcgctgaa tgactatgtt ctggaggcag gaaaggtgca
      781 cgcagatgac actccggtga aagtactggc cccggggaac ggaaagacga aaacgggtcg
      841 tctgtgggta tacgtcaggg atgatcgtaa tgcgggttca tccctgccgg cagccgtctg
      901 gttcgcgtat tcggca
//
LOCUS       DADLXX010000337          908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLXX010000337 DADLXX010000000
VERSION     DADLXX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.344"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>908
                     /locus_tag="JNX27_004307"
     CDS             <1..>908
                     /locus_tag="JNX27_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7885218.1"
                     /translation="TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTL
                     WPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTC
                     GGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLV
                     LKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKT
                     GETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEP
                     VAKVCYAQMVKQFLSRDPFECVLCGGR"
BASE COUNT          207 a          226 c          298 g          177 t
ORIGIN      
        1 actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg
       61 atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg
      121 ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaatga cgtgtgccgt
      181 ctggcggtgg agaatctgct gtatgccgcc cggaaacggg ggcaggaacc cggtatcttc
      241 tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc cgcatgtaca tgtgtctgta
      301 acctgtggag gtctgaataa gcatggtcag tggaaaaagc tgagcttcct gaaagacgcg
      361 atgcgttcgc ggtggatgtg gaatatgcgg caacggcttc tgaaagcgtg gtcagagggg
      421 ctggcgatgc cggagtcgtt gtcacatatc acgacggaat cacagaggag aagcctggtg
      481 ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga aaaaaacggc cggcgggagg
      541 aatacggcgc gctacctggg tcgttatctg aagaagccgc cgatagcggc ctcccggctg
      601 gcacattaca acggaggggc gagcctgagc ttccgttacc tggaccacaa aacgggagaa
      661 acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca gcacatcccg
      721 gagaagtttt ttaagatggt gaggtacttc gggttccttg ccagccgggt gtgtggagag
      781 aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt ggcgaaagtg
      841 tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg cgtgctgtgt
      901 ggcggccg
//
LOCUS       DADLXX010000338          894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLXX010000338 DADLXX010000000
VERSION     DADLXX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.345"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(110..439)
                     /locus_tag="JNX27_004308"
     CDS             complement(110..439)
                     /locus_tag="JNX27_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7885219.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..>894)
                     /locus_tag="JNX27_004309"
     CDS             complement(611..>894)
                     /locus_tag="JNX27_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885220.1"
                     /translation="FVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          217 a          207 c          199 g          271 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 ctatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaat
//
LOCUS       DADLXX010000339          881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLXX010000339 DADLXX010000000
VERSION     DADLXX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.346"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..691
                     /locus_tag="JNX27_004310"
     CDS             <1..691
                     /locus_tag="JNX27_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7885221.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            793..>881
                     /locus_tag="JNX27_004311"
     CDS             793..>881
                     /locus_tag="JNX27_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7885222.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALR"
BASE COUNT          198 a          198 c          220 g          265 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctgttag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgttt ggtgccagtt atcgctacag tggtgctgga gtcggttatc aggttgccag
      541 tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt actttgccgg
      601 gaactggcat agcatcgcca tttatttgct ggctggatgc ctgatttctg caatgaccgc
      661 tttattgatg aaagacaatc aacgctcgtg atagcctgcc gaagatcatc cgatcttcgc
      721 cttatacttt tgtttcacat tcctgtgaca tactatcgga tgtgcggtaa ttgtatggaa
      781 caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc actggataaa
      841 ctcgacgcgc tgtatgagca atctgtagtt gctttacgca a
//
LOCUS       DADLXX010000340          863 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLXX010000340 DADLXX010000000
VERSION     DADLXX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.347"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..256)
                     /locus_tag="JNX27_004312"
     CDS             complement(<1..256)
                     /locus_tag="JNX27_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885223.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
     gene            281..520
                     /gene="relB"
                     /locus_tag="JNX27_004313"
     CDS             281..520
                     /gene="relB"
                     /locus_tag="JNX27_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY7885224.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            520..807
                     /gene="relE"
                     /locus_tag="JNX27_004314"
     CDS             520..807
                     /gene="relE"
                     /locus_tag="JNX27_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY7885225.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          240 a          175 c          219 g          229 t
ORIGIN      
        1 acatgctaaa gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca
       61 caaagtatat ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac
      121 ctgcacaata caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca
      181 ccgttcttac gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg
      241 atacttgtaa tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg
      301 tattgacgat gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc
      361 ttctgaagcg cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa
      421 acagacactc ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg
      481 taatcctaag ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt
      541 gacgagcggg cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa
      601 aagaagctgg ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg
      661 cctgattgtt acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata
      721 gacgagaaag ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat
      781 agcgaggcgg tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag
      841 gtgacacttc tgctttgcgt tga
//
LOCUS       DADLXX010000341          853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLXX010000341 DADLXX010000000
VERSION     DADLXX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.348"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..761
                     /locus_tag="JNX27_004315"
                     /pseudo
     CDS             1..761
                     /locus_tag="JNX27_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          231 a          184 c          203 g          235 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cag
//
LOCUS       DADLXX010000342          850 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLXX010000342 DADLXX010000000
VERSION     DADLXX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.349"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            58..555
                     /locus_tag="JNX27_004316"
                     /pseudo
     CDS             58..555
                     /locus_tag="JNX27_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            596..787
                     /locus_tag="JNX27_004317"
                     /pseudo
     CDS             596..787
                     /locus_tag="JNX27_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          219 a          210 c          249 g          172 t
ORIGIN      
        1 cgccagcccc gtcccgcccg atagagcgcg gcatagcggg ccccggatta cttgcgtgcg
       61 acggcagaag aagccctgaa gcgaatcagt gaactgtacg ccatcgagga tgaaatacgg
      121 ggattaccag agtcagagtg tcttgcagtc aggcagcagc gaagcaaagc gttactgacg
      181 tcgctgcatg aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagactgggc
      241 gaagcattca gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt
      301 ctggcggagg cggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag
      361 aaaaattacg tgttcttcgg tagcgatcac ggcggcgagc gtggtgcact gctgtacggg
      421 ctgatcggca cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg
      481 agcgtactgc cggaatggcc ttccaaccga gttgacgacc tcctgccatg gaaggtggtg
      541 ctaccatcag gataatcgtc aatacggtgt tcggtgaacg tttacttgga gcatcatgct
      601 tcatgaagaa aaactggcac gtcatcaacg taaacaggcg atgtataccc gaatggcagc
      661 cttcccggcg gtgaaaatgt tcgaagagta tgacttcacg ttcgccaccg gagcaccgca
      721 gaagcagctc cagtcgttac gctcactcag cttcatagag cgcaatgaaa acatagtctt
      781 actggggaca tctgacatca caaatccccg ggtggggatt tgtgtataag agacagccca
      841 tggaggaagg
//
LOCUS       DADLXX010000343          842 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLXX010000343 DADLXX010000000
VERSION     DADLXX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.350"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..173)
                     /locus_tag="JNX27_004318"
     CDS             complement(<1..173)
                     /locus_tag="JNX27_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7885226.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVS"
     gene            complement(170..532)
                     /locus_tag="JNX27_004319"
     CDS             complement(170..532)
                     /locus_tag="JNX27_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7885227.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          256 a          174 c          167 g          245 t
ORIGIN      
        1 tcagaaacag ccagagcgtt aatgattgac tccgtagcgt ctttgcttcc gtcatttatg
       61 aaaacaattt ctacttcata tggcttcaac tcttcgaatt cacgtaccgt tttataaaaa
      121 attggtatcg cttcttcttc attgaagaca ggaacgacaa gagatatctt catttcgcat
      181 ccctaaagac aatgaacttt gaatagacga aaccgcacac caagctgatg gcggagaagg
      241 taacaagagt gacaattgga ggaagtgaac atctatcagc aacccatcca acagtagcac
      301 tcagtgttcc catgaacccg acatataaca tgtagcgcat cgttgtagtt gatgctttga
      361 atgtgaattt tgcattcgca aagaagctaa aactcacagc cacaacgaaa cctgcgaagt
      421 ttgccatagc ctgattggta tgcgcggcat agatacatac accaaaaacc acccagtgta
      481 taagtgtgtt cagcacgcct atagatgtgt actttgcaaa tagctttaac atttcttcta
      541 tcagctaata atcaaaggca tgaagtctat catccaagtc tcaatcgatc gatacttgcg
      601 gtagttgatg agaaaaactc tggcacacaa atctttgcac tggattgcaa ggctttgtgc
      661 tcttctgtga atgtgtggct atatgtttga agatcgttgt gccgtatttg tgacatatat
      721 atggcaacat cattcatcaa ctttctgttt gtgccatcaa ctatatctta gtgaatgcgg
      781 ttaatgcttg ctaaaacaga tagttatgat tggtgctaca gattcgtaat gcgaaggtcg
      841 ta
//
LOCUS       DADLXX010000344          841 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLXX010000344 DADLXX010000000
VERSION     DADLXX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.351"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            53..841
                     /gene="virF"
                     /locus_tag="JNX27_004320"
     CDS             53..841
                     /gene="virF"
                     /locus_tag="JNX27_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7885228.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          306 a          116 c          137 g          282 t
ORIGIN      
        1 tcttagttac tctgtaaaca ctaaatatag tttggtatat tctgttgaat ttatgatgga
       61 tatgggacat aaaaacaaaa tagatataaa ggttcgcttg cataactata ttattttata
      121 tgcaaaaagg tgttcaatga cggttagctc aggcaatgaa actttgacta tcgatgaagg
      181 gcaaattgct tttatagagc gaaatataca aataaacgtc tccataaaaa aatctgatag
      241 cattaatcca tttgagatta taagccttga cagaaattta ttattaagca ttattagaat
      301 aatggaacca atttattcat ttcaacactc ctattctgag gagaaaaggg ggttaaacaa
      361 aaaaatattc ctcctctctg aggaggaggt ttctatcgat ttgttcaaat ctataaaaga
      421 gatgcctttc ggcaaaagaa agatctatag tttagcttgc cttttatcag ctgtttctga
      481 tgaggaagct ttatatactt cgatatcgat agcttcttct cttagttttt ctgatcagat
      541 aaggaagatt gttgaaaaaa acatcgagaa gagatggcgt ctttctgata tttcaaataa
      601 cttgaattta tcagaaatag ctgttagaaa acgattggag agtgaaaaat taacatttca
      661 acaaatcctt cttgatattc gcatgcatca tgcagcaaag cttttattga atagtcaaag
      721 ctatattaat gatgtatcaa gacttatcgg aatatcaagc ccatcttatt ttataaggaa
      781 atttaatgaa tattatggta taactccaaa gaaattttac ttatatcata aaaaatttta
      841 a
//
LOCUS       DADLXX010000345          837 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLXX010000345 DADLXX010000000
VERSION     DADLXX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.352"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            143..>837
                     /locus_tag="JNX27_004321"
     CDS             143..>837
                     /locus_tag="JNX27_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY7885229.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLHELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGG"
BASE COUNT          223 a          215 c          225 g          174 t
ORIGIN      
        1 actcttttat caacgcttac gtaattaagg gacacagcct aaatagctgc gcggaatagt
       61 agatcccttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
      121 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      181 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      241 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      301 ccgttcctcg gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      361 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttaca
      421 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      481 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      541 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      601 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      661 agagcatccg gtcttttatg aagatgaagt ggatatccat cttcatccca aaatcggtgc
      721 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      781 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctatgtgg gcggcaa
//
LOCUS       DADLXX010000346          833 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLXX010000346 DADLXX010000000
VERSION     DADLXX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.353"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..331
                     /locus_tag="JNX27_004322"
     CDS             <1..331
                     /locus_tag="JNX27_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885230.1"
                     /translation="GWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDV
                     RYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGT
                     WYNVASI"
     gene            391..>833
                     /locus_tag="JNX27_004323"
     CDS             391..>833
                     /locus_tag="JNX27_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7885231.1"
                     /translation="MTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSS"
BASE COUNT          245 a          177 c          220 g          191 t
ORIGIN      
        1 aggctggcat tttttcagcc agagaaaccc ggataacagc atagtgttca ctgttaacgg
       61 acaggtaatt ccgttaaatt acggaaactt cgatgcccgc tataaatatc gaacagaggg
      121 ggtacaggat gtacggtatg gccatgaaat gtattacagc cccggcagta acaccgtttc
      181 gtggagattt tgcgcacctt cgggacacgg gctgtcaggg atggcgatat cggataccgg
      241 ccgtaactca gcggataacg ttgacggtgt gtattaccga ccactgcaaa aactgattaa
      301 tggcacctgg tataacgtag cgagtattta acaatgttgc atttaaaaaa tattactgcg
      361 ggtaatccga aaacagcaga acaatatcag atgacaaaac gatattcggt cacctggctt
      421 ttttcagaag acggaaaaaa ctggtatgaa gagctgaaga atttcgccag cgacacaata
      481 aaaatagctt acaccggaga tggtcgcgtg gtgtgggtcg gtaaggatgt gacaggcatc
      541 gagccacgca atgccagcgt tatcgaagtt ccggatatta ccgctaaccg acggattacc
      601 gcgccgggtt actggtttta ccgcaatgat gaatttgtct ttgactacag actcaaagcg
      661 gaagatgagc gtgatgccct tctggctcag gtcagtgccc ggacagggga atgggaagaa
      721 gacctgctgc tggggttaat cagcgacgaa gataaagaaa agctgaaagc ctgtcgtatt
      781 tacgcgaaat cgctgcaggc gatggatttc agcaccatca ctgataaatc ctc
//
LOCUS       DADLXX010000347          830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLXX010000347 DADLXX010000000
VERSION     DADLXX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.354"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..326
                     /locus_tag="JNX27_004324"
     CDS             <1..326
                     /locus_tag="JNX27_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7885232.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNX27_004325"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNX27_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY7885233.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          254 a          181 c          155 g          240 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca ggcgaccttc tgcatgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gggtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagag cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gaagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DADLXX010000348          818 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLXX010000348 DADLXX010000000
VERSION     DADLXX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.355"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..269)
                     /locus_tag="JNX27_004326"
     CDS             complement(<1..269)
                     /locus_tag="JNX27_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885234.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRH"
     gene            complement(271..549)
                     /locus_tag="JNX27_004327"
     CDS             complement(271..549)
                     /locus_tag="JNX27_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885235.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            490..675
                     /locus_tag="JNX27_004328"
     CDS             490..675
                     /locus_tag="JNX27_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885236.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(717..>818)
                     /gene="hokD"
                     /locus_tag="JNX27_004329"
     CDS             complement(717..>818)
                     /gene="hokD"
                     /locus_tag="JNX27_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY7885237.1"
                     /translation="IVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE"
BASE COUNT          183 a          223 c          234 g          178 t
ORIGIN      
        1 atatggcgca acagccagtc agacaatgcg ccagcaccac cagcagcacg aatcacccgt
       61 gcgttactga aaaacggcag caatgttttg tcttccacca gcggctggcg aacggcagga
      121 acgaccccgg acggcagatt acgcatgctt ttcggttccg gctccaccag tacccgggta
      181 ttgtggaata ccggcatgga ttcacggcct ggcttaagga ccaccagccc gagttccgga
      241 accagaacag gtcgaagtaa tacccgcaca ttacctccag atccgttgct ggaatgtgca
      301 ggacggacgc ggtggccgtt cggaataagg gagcctgacg aagattatcc agtgacgata
      361 atcgagactg agggctttct taatctcgta tccgtgtctg cggtagcgct gaatcagcca
      421 ttcagcctgt tcttcggtgc aggggtcgtg ctgataccag tcagatttga atgcatgaga
      481 acgccgcccg tgcctgctgg caggggcggc agagttatcc gaattgtaaa atttggtatc
      541 gtgcgccatc tgttttctct gctggcgcag caggtgccag ttgttcaggc tgacggatgg
      601 attgtaaacc agaacgacca gaaaaaacaa aacccgccga agcgggttaa gtgcgggtgc
      661 gttgaggatg cctgacacat cagcggtggc gagggatttc tcccccgccg ggtctcttac
      721 tcctcaggtt cgtaagctgt gaagacagcg acctccgtct ggccggttcg gattcgtacc
      781 tcgcagaggt ctttcctcgt taccagtgcc gtcactat
//
LOCUS       DADLXX010000349          787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLXX010000349 DADLXX010000000
VERSION     DADLXX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.356"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..200
                     /locus_tag="JNX27_004330"
     CDS             <1..200
                     /locus_tag="JNX27_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885238.1"
                     /translation="PTTGSTSPAVPFQITFSECSKGTTGVRVAFNGIEDAENNTLLKL
                     DEGSNTASGLGKKYWTEICVR"
     gene            188..355
                     /locus_tag="JNX27_004331"
     CDS             188..355
                     /locus_tag="JNX27_004331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7885239.1"
                     /translation="MRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            368..733
                     /locus_tag="JNX27_004332"
                     /pseudo
     CDS             368..733
                     /locus_tag="JNX27_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT          223 a          167 c          193 g          204 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgttt agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat ggagggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa
      781 ggtgctt
//
LOCUS       DADLXX010000350          756 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLXX010000350 DADLXX010000000
VERSION     DADLXX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.357"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="JNX27_004333"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="JNX27_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7885240.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="JNX27_004334"
     CDS             103..375
                     /locus_tag="JNX27_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7885241.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            602..>756
                     /gene="ghoS"
                     /locus_tag="JNX27_004335"
     CDS             602..>756
                     /gene="ghoS"
                     /locus_tag="JNX27_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7885242.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLN"
BASE COUNT          236 a          128 c          186 g          206 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggc
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 atcaatcggg gttaataagt tttgcgtccc cagagcgttt aatattgata ggagtcatat
      601 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
      661 ttcctcagtg ttcttaagat taagatcatt gatgtatgat atgaatttct cctctatcgt
      721 ggctgatgaa tatgggatac cacgacaatt gaatga
//
LOCUS       DADLXX010000351          748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLXX010000351 DADLXX010000000
VERSION     DADLXX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.358"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            151..748
                     /locus_tag="JNX27_004336"
                     /pseudo
     CDS             151..748
                     /locus_tag="JNX27_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          202 a          198 c          208 g          140 t
ORIGIN      
        1 gcgcaggatg tggcggcagc cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa
       61 gcccgacagg atgcaaccgt cacagctgag gacagaaagg ccactgcgga agatgtgaca
      121 agcacaggag caaatgcagc cgcagccgga cagagcgcac aggatgccgc aggttatgcc
      181 cgcgcagcag aacaggccaa aaatgacatt gatgctgcgc tgaccggcac cctgaaaacg
      241 gctaaccatc tgtctgaaat cgcagcagca ggcgaaaagg cacaacagaa gtcccgggat
      301 aatctggggc tgaaaagtgc ggccacgatg gaagcacaga gcgacattta cgaccggaca
      361 aaaggccgtc tggcgatacc cggcgcattc ggctttgggt gtgcttttct gcctgaagat
      421 gttatccgtt ttgacactaa gagtgatttc ctggcctggg taaggaatgc gctgccaggt
      481 gaatattccg ttgctggccc ttacggcatc atcatacccg acacacggtt tgaaggggtg
      541 ctcagcatcc ggtggactga tgcacgccct gagacaacag aaccgcggta cagagccaaa
      601 tcccttactt tttacggcat taacggcccc atttatcata cccgctactg ctactggccc
      661 atatccagac tgactggctg ggtgaaaata aatataacca cagaagatat tatttacaga
      721 atcgtggcga gctctgtccg caacagat
//
LOCUS       DADLXX010000352          736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLXX010000352 DADLXX010000000
VERSION     DADLXX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.359"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..71)
                     /gene="ospE1"
                     /locus_tag="JNX27_004337"
     CDS             complement(<1..71)
                     /gene="ospE1"
                     /locus_tag="JNX27_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY7885243.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNP"
     gene            complement(180..403)
                     /locus_tag="JNX27_004338"
                     /pseudo
     CDS             complement(180..403)
                     /locus_tag="JNX27_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          210 a          128 c          178 g          220 t
ORIGIN      
        1 ggatttgata cttcaaggat gatattttct tgtttttgtg gaaggcaagg gaatatagtt
       61 tgtgtaagca tattacttcc ttatgttata ttttggtaaa aaatttattt ttttacatta
      121 tagtcggcta aatgaagttt actgtaaaaa aacgatactt tttcgggtgg cgcttccaat
      181 cagtagaata tgtgtttttc aatgaaggag ccgatgactt ttcgtggatt tccacggaac
      241 gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg gtcaggttgt
      301 tacgttcaag atatagcatg tgctggatga agatcccttt tctggtcacc tgtttatctt
      361 tcgtggccga cggggtgaca ctgttaaaat gtaatgaacg catcccacac aattaaacac
      421 ggagtagtat gatggttaaa agggtgaaac tggcaggtgt ggagcctcgc agctttccgg
      481 catcatactg cccaatacgg gatgcataaa gcgtcctcag actggagact ccacaaatga
      541 aatatacacc ggttggcgtt gatatggcaa aacatgtcat tcagattcac ttcatcaatg
      601 agcacatgtc agcgccagtg atataagacg gtaatgggga tgcggacgat ttcctggact
      661 ctcagaagat gaaacatgtt tactgaagaa caaaaaattc gcgctattga gctttatttc
      721 aaatatggta agaagc
//
LOCUS       DADLXX010000353          731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLXX010000353 DADLXX010000000
VERSION     DADLXX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.360"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>731
                     /locus_tag="JNX27_004339"
     CDS             <1..>731
                     /locus_tag="JNX27_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY7885244.1"
                     /translation="HYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHG
                     ISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPN
                     YLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMK
                     ALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERF
                     FRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          176 a          184 c          226 g          145 t
ORIGIN      
        1 cattatcctg tggtcacact ctgccatgtg ttcggggttc atcgcagcag ctacagatac
       61 tggaaaaacc gtcctgaaaa accagacggc agacgggctg tattacgcag tcaggtactt
      121 gagctacatg gcatcagcca cggttcggcc ggagcaagaa gcatcgccac aatggcaacc
      181 cggagaggct accagatggg acgctggctt gctggcaggc tcatgaaaga gctggggctg
      241 gtcagctgtc agcagccgac tcaccggtat aaacgtggtg gtcatgaaca tgttgctatc
      301 cctaactacc ttgaaaggca gttcgccgtg accgagccaa atcaggtgtg gtgcggtgat
      361 gtgacctata tctggacggg taagcgctgg gcgtacctcg ccgttgttct cgacctgttc
      421 gcaagaaaac cagtgggctg ggccatgtcg ttctcgccgg acagcaggct caccatgaaa
      481 gcgctggaaa tggcatggga aacccgtggt aagcccggcg gggtgatgtt ccacagcgat
      541 cagggcagtc attatacgag caggcagttc cggcagttat tgtggcgata ccagatcaga
      601 cagagtatga gccggcgcgg aaactgctgg gataacagcc caatggaacg cttcttcagg
      661 agtctgaaga acgaatggat gccggtggtg ggttacgtaa gcttcagcga ggcagctcac
      721 gccataacgg a
//
LOCUS       DADLXX010000354          727 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLXX010000354 DADLXX010000000
VERSION     DADLXX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.361"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            119..>727
                     /locus_tag="JNX27_004340"
     CDS             119..>727
                     /locus_tag="JNX27_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885245.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVSHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPD"
BASE COUNT          151 a          198 c          217 g          161 t
ORIGIN      
        1 tcgcccgctg cacggagcgg gaatttgcga ccattcttgc cggtatcagc gccggcggta
       61 tgtactgccc ggagtgtggc acggttcact ggccggacgg agtcgccccg cccttctgat
      121 gcttccccgt tttgccgata tttttcagca gggaaaccgc tggcttaact ggctggagaa
      181 acaaccggaa ggggcagtcc gtccggtggt aattgaatca gtgacaaaaa tcatggcctg
      241 cgggaccaca ctgatgggct acacgcagtg gtgttgttca tcaccggact gttgccatac
      301 aaaaaaggtc tgcttccggt gtaaaagccg ctcctgcccg cactgcgggg tgaaggctgg
      361 cgcacagtgg atacagtatc tgctgagcct ggtccccgac tgcccgtggc agcatattgt
      421 gttcacactt ccctgccagt actggtccct ggtgttccac aaccggtggt tactggcaga
      481 gatgagccgc attgcagcgg atgtgatact ggaaatctgc catcaggcag atgtggagcc
      541 ggggatattc acggtgagcc acacatgggg gcgtgaccag cagtggcatc cgcatatcca
      601 tttatcgaca actgccggtg gtgtgacgtc gggccacacc tggaaaaatc ttcattttta
      661 cgcccgtaag gtgatgagca tgtggcgcta ccggataaca cggttgctgt cacggaaata
      721 ccctgac
//
LOCUS       DADLXX010000355          712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLXX010000355 DADLXX010000000
VERSION     DADLXX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.362"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            278..>712
                     /locus_tag="JNX27_004341"
     CDS             278..>712
                     /locus_tag="JNX27_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7885246.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDVATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          217 a          122 c          147 g          226 t
ORIGIN      
        1 atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata
       61 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
      121 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
      181 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
      241 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
      301 gtttataact gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac
      361 ggatggctca gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      421 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      481 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      541 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      601 tgccaaccag gctaccaccg atgtagcgac gaatgttgct ctacaaatgt atctgccaga
      661 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cg
//
LOCUS       DADLXX010000356          699 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLXX010000356 DADLXX010000000
VERSION     DADLXX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.363"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..311)
                     /locus_tag="JNX27_004342"
     CDS             complement(<1..311)
                     /locus_tag="JNX27_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7885247.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     M"
     gene            complement(375..>699)
                     /locus_tag="JNX27_004343"
     CDS             complement(375..>699)
                     /locus_tag="JNX27_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7885248.1"
                     /translation="RNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTD
                     NKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVG
                     VGYKF"
BASE COUNT          165 a          233 c          155 g          146 t
ORIGIN      
        1 ttcatcacct ccggacgtaa atcaccatcc tttggtgcat tcagaaacgc gttcagtgtc
       61 cccggtgcat cagtcggtgc cacatcaata tccccgacac gggttggctg ccagccgtta
      121 cagtgaagcg caacctcgta ataccccggc tcagcctcaa tcacataagc accgttattg
      181 tccgtcacgc aagtggcaac aacgtgtgcc acaacggtcg gactggttct tctggcccgc
      241 agttcaatcg cacaatttac gacaggttta cccgccccat ctttcagtac acctgaaatc
      301 tttactgcca tattcacccc acaaaaagcc cgcctgaacc agcgggctgt cataacactg
      361 tgttacctgg ctaatcagaa cttataaccg acacccacaa tgaaaccgtc agtgcgccag
      421 tcgccactgc cggaaccttc ataagcaagg tcaatggcca cggattcggt cgggttaaac
      481 tgcacgccag ccccccacgc cagagacgtg ttgctgtggc ggttgtcatc actcccggtc
      541 agcacatcgt gcgttttccc cttgttatca gttacgcgga gataatcccc ggagaaggtc
      601 gacacacggc tgtaagccac gcccgccatc gcatacgcgc tgaaccattc attcacgcgc
      661 acagacggcc ccgccatcac gctgaaccag cggttacgc
//
LOCUS       DADLXX010000357          686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLXX010000357 DADLXX010000000
VERSION     DADLXX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.364"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..573
                     /locus_tag="JNX27_004344"
     CDS             <1..573
                     /locus_tag="JNX27_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HAY7885249.1"
                     /translation="RHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNPVID
                     YVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKA
                     TLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTV
                     EILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          211 a          139 c          158 g          178 t
ORIGIN      
        1 cgacacaata atagaaatgt gatttttctg gttccccatg gctggagcgt ggatattcct
       61 gcaatgttgc tggcagctca gggaaaaaaa atggccgcca tgtttcatca gcaacgaaat
      121 ccagtgattg attatgtctg gaattcagta cggcgtaaat tcggggggcg cttacatgcc
      181 cgggaggatg ggataaaacc atttattcag tcagtacgcc agggatactg gggatattac
      241 ctcccagatc aggatcatgg tcctgaatac agtgaatttg ctgatttttt tgcgacctat
      301 aaagcgacat taccaatcat tggacgtctg atgaacatca gtcaggctat gattatacca
      361 cttttcccgg tttatgatga aaaaaaacat ctactgacta ttgaaattcg gccaccaatg
      421 gatgcttgca ttgccagcgc ggacaataaa acgattgccc gacaaatgaa caaaacagtg
      481 gaaattttgg tggggccaca tccggaacag tatgtctggg ttttaaaatt gttaaaaaca
      541 cgcaaatcaa acgaagcgga cccgtaccct tgaaattata aagtacaagt atttccacta
      601 gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat ctgacgaatt agacaaagtg
      661 gtgtccacca aataagtagt gggaac
//
LOCUS       DADLXX010000358          680 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLXX010000358 DADLXX010000000
VERSION     DADLXX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.365"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..198
                     /locus_tag="JNX27_004345"
     CDS             <1..198
                     /locus_tag="JNX27_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7885250.1"
                     /translation="FLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIGIGVLGE
                     YIGRIYIEVKNRPKYIIKDKK"
BASE COUNT          220 a          108 c          107 g          245 t
ORIGIN      
        1 tttttatatg gtgcatggat gattatagac acccttgtct ttggtaaccc agtacgcggg
       61 tatccctccc tgcttgtatc aatacttttc ttgggtggcg tgcaactgat cgggattggt
      121 gttctcggag aatatatagg cagaatatat attgaagtaa aaaatagacc taaatatatt
      181 attaaggata agaaatgatt aaatcaaatt acatatgctt tcttttgctt tttttattgg
      241 tttaactatt cttcatttaa atcaatatac actagtatca gatgacattt ggttttccga
      301 gaaatcaata tcagaaccgg atcacatcaa gtggcttata gatagatatt tcacatggtc
      361 atcacgaaca tagattgaat atgcattaat aaatcttatt aatcattttt atgcttggtc
      421 tttaattaat gcagcaatgt tttcatcatt aattattggc atttgctcta tctttcagag
      481 aaaaaacaac ctttactata aaataaacac ttcaatcatg tcattattac tgattgttat
      541 gatgccaagc gatgtttttt tcaatgccgc gatttggata acaggatcat tcaactatct
      601 gtggccaagt gctattgcat ttattggtta ttctctactt ataaaaaaaa tcaatatgga
      661 aaaccacgag aaaactcaat
//
LOCUS       DADLXX010000359          660 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLXX010000359 DADLXX010000000
VERSION     DADLXX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.366"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            104..>660
                     /locus_tag="JNX27_004346"
     CDS             104..>660
                     /locus_tag="JNX27_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7885251.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          238 a          121 c          109 g          192 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacaaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgcctta cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatagtttct ggaaacagat tagacagcat
//
LOCUS       DADLXX010000360          636 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLXX010000360 DADLXX010000000
VERSION     DADLXX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.367"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            88..153
                     /locus_tag="JNX27_004347"
                     /pseudo
     CDS             88..153
                     /locus_tag="JNX27_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JNX27_004348"
     CDS             381..629
                     /locus_tag="JNX27_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7885252.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DADLXX010000361          623 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLXX010000361 DADLXX010000000
VERSION     DADLXX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.368"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>623
                     /locus_tag="JNX27_004349"
     CDS             <1..>623
                     /locus_tag="JNX27_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7885253.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTTGGFNSETVQDIMLGAVERRFGNELPASPVEWLTDN
                     GSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT          149 a          152 c          195 g          127 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actacgggcg gcttcaacag tgaaacagta caggacatca tgctgggagc
      421 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
      481 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
      541 gaagagcacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
      601 gataaagcgt gactacatca gtg
//
LOCUS       DADLXX010000362          615 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLXX010000362 DADLXX010000000
VERSION     DADLXX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.369"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNX27_004350"
     CDS             complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNX27_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY7885254.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSYFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          187 a          122 c           91 g          215 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa ataactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgtt
//
LOCUS       DADLXX010000363          596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLXX010000363 DADLXX010000000
VERSION     DADLXX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.370"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..323
                     /locus_tag="JNX27_004351"
     CDS             <1..323
                     /locus_tag="JNX27_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885255.1"
                     /translation="ARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDT
                     PEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMK
                     GGIR"
     gene            320..>596
                     /gene="tnpB"
                     /locus_tag="JNX27_004352"
     CDS             320..>596
                     /gene="tnpB"
                     /locus_tag="JNX27_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7885256.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT          145 a          157 c          172 g          122 t
ORIGIN      
        1 ttgcccggat cgccagagaa cacgatatca ataataacct gctgtttaaa tggaaaagcc
       61 agtacgagga cggcttactg agcgatgatg acatacagga atgcatgcct gtcccggtgg
      121 cactgactga tacgccggag ccgaccagac cagttacaaa tcccttctgg cgtaacaagc
      181 atgatgagcg ccctgagggg gctcccggaa acgtcccacg gtgcgagctg catcttaaat
      241 caggtgtggt aaaactgttt gaccctctca ctccggaact gttacgggcg ctaatccgcg
      301 aaatgaaagg gggtatccga tgataacgct gccgaccggt accaaaatct ggatcatcgc
      361 tggcatcaca gatatgcgtt gtggcttcaa tggcctggct tcgaaggtgc agaacacgct
      421 gaaagatgac ccgttctccg ggcatatctt cgtcttccgg ggccgcagtg gcaaaatggt
      481 gaaaatactg tgggccgatc gtgacgggtt atgcctgttc gccaaacgcc tggaacgggg
      541 ccgcttcgtc tggccggtga cccgggaagg gaaagtgcac ctgacgccag ctcagt
//
LOCUS       DADLXX010000364          593 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLXX010000364 DADLXX010000000
VERSION     DADLXX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.371"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>593
                     /locus_tag="JNX27_004353"
     CDS             <1..>593
                     /locus_tag="JNX27_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7885257.1"
                     /translation="VTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGE
                     LRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTL
                     YTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQER
                     AEQHIDLAYAIIAHGAQGASETFAIALEGTEGNRKLM"
BASE COUNT          132 a          156 c          199 g          106 t
ORIGIN      
        1 tgtcacgcac ctgaatgagg accggcgcgt actgaacagc atgattcatg atgcacggga
       61 aaaggccgga gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat
      121 acgcgacggg gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct
      181 ggtggataat gtgtatcacc ggattgccgg tatcagcaag gatgacgggc tgataaccct
      241 gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt
      301 cacgctgtac accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa
      361 gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg
      421 tgacagtgtc acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg
      481 ggcagagcag catattgacc tggcctatgc catcatcgcc cacggtgcac agggggcgag
      541 tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tgg
//
LOCUS       DADLXX010000365          579 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLXX010000365 DADLXX010000000
VERSION     DADLXX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.372"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            97..>579
                     /locus_tag="JNX27_004354"
     CDS             97..>579
                     /locus_tag="JNX27_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY7885258.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQGNAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     L"
BASE COUNT          157 a          119 c          171 g          132 t
ORIGIN      
        1 tatcgacgac gagcagtttg atgaaaccaa tattgtgcgc ggactgcctg ttcctggtgg
       61 tcggttgggg ataaaaatca cagagctgga gtgtgcatga ataaccagta tttacagttt
      121 gttcgtgagc agctcattat cgccaccgct gatttgagtg gggcgacaaa aggtcagctt
      181 gaagcctggc aggggaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg
      241 taccgcgatg aggtgaccgg aaaaatgata acgcgggata atccaccaat cccgggaaaa
      301 caatcactgg cgaagggggc gtcaattcct ctggtcagtc aggttgcgtt ttcgacatca
      361 tcgtggcggc gggctgttct gtctcttgaa gagcaccata aagcctggtt gttgtggtgt
      421 tacagcggaa gtatttgctg ggagtatcag atcaccataa cccactgggc gtgggaagag
      481 tttaaggctc attctggcac taggaaaatt gcagagaaaa cacaggaacg cctgaaaaaa
      541 ttaatctggc tggcggcgca ggcggtaaaa gcagaactt
//
LOCUS       DADLXX010000366          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLXX010000366 DADLXX010000000
VERSION     DADLXX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.373"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..297
                     /locus_tag="JNX27_004355"
     CDS             <1..297
                     /locus_tag="JNX27_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7885259.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JNX27_004356"
     CDS             complement(405..>566)
                     /locus_tag="JNX27_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885260.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLXX010000367          553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLXX010000367 DADLXX010000000
VERSION     DADLXX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.374"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(28..>553)
                     /locus_tag="JNX27_004357"
     CDS             complement(28..>553)
                     /locus_tag="JNX27_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7885261.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGAAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          144 a          139 c          134 g          136 t
ORIGIN      
        1 accagtattt cttcggagtg aagaatatta ccaagtatat ttaacaccca cgttcgcgga
       61 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
      121 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagcccctt cagtaccgtt
      181 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
      241 tttgaagtac ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac
      301 acccagttca taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag
      361 ctggtagtca tcaccagact ggaacagacc agaaatgctg ccgtaaggag tcacgtaacc
      421 agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg aagccccaag cgtcggagtt
      481 ggtgctaccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
      541 agagtagctc aag
//
LOCUS       DADLXX010000368          539 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLXX010000368 DADLXX010000000
VERSION     DADLXX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.375"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            56..>539
                     /locus_tag="JNX27_004358"
     CDS             56..>539
                     /locus_tag="JNX27_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885262.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYRRMLYIEFTDNMR
                     Y"
BASE COUNT          134 a          137 c          137 g          131 t
ORIGIN      
        1 caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag agcttatggt
       61 cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga gtagccgggc
      121 gattgccaga gaactggggc tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc
      181 tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg aataccggga
      241 ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa cggtaatcgc
      301 tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg cattcattcg
      361 ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg aacccggacg
      421 acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt
      481 tgctgttccc ggatacagac gaatgttgta catcgaattc actgacaata tgcgttatg
//
LOCUS       DADLXX010000369          538 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLXX010000369 DADLXX010000000
VERSION     DADLXX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.376"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..276
                     /locus_tag="JNX27_004359"
     CDS             <1..276
                     /locus_tag="JNX27_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7885263.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGFDNGGESAA
                     IIYSLLFTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          129 a          118 c          143 g          148 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttttga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg ttcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatctag gttgtgtccc ttaattacgt aagcgttg
//
LOCUS       DADLXX010000370          525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLXX010000370 DADLXX010000000
VERSION     DADLXX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.377"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>525)
                     /locus_tag="JNX27_004360"
     CDS             complement(<1..>525)
                     /locus_tag="JNX27_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7885264.1"
                     /translation="CADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVV
                     DVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTI
                     TVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDVA
                     LAVVAHPNADNAMQ"
BASE COUNT          149 a          124 c          105 g          147 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaat
//
LOCUS       DADLXX010000371          521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLXX010000371 DADLXX010000000
VERSION     DADLXX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.378"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            452..>521
                     /locus_tag="JNX27_004361"
     CDS             452..>521
                     /locus_tag="JNX27_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7885265.1"
                     /translation="MEPVYVILNALLDSGRFTRKLIL"
BASE COUNT          119 a          128 c          143 g          131 t
ORIGIN      
        1 acccgattag ttgttaatca tatttaatca tagtgattac tttcagacgg aacaaggcaa
       61 ataatgcgag tcagcgcata taaggcggca tacggtggtt acatgtagca caggtgtaaa
      121 accgccgaag cccggcgcaa gccggtacgg tcgtccgaat atcaccagtc cccctccggc
      181 tctgcgaggc catgcggcct gtcagcgatg aaaatgcctc tgtgcgcggt cggagtggtc
      241 ccgacaaggg tttacccgca gtcggggcgt atctccgcgt tagcgggccg tgagggccgc
      301 ttacgagcgt gtatgagaga cgtacagtag ttttgtccgc tcacaccagt acaaaccagt
      361 cgaaataccg gtaacgacga ttactcttgt ctctgatgcc ggtggactac gagattttat
      421 ttactggcgt cctgatgcgg tcaggactgg tatggaacct gtttatgtga tacttaatgc
      481 tcttctggat tccgggcgat ttacgcgtaa acttattctg t
//
LOCUS       DADLXX010000372          513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLXX010000372 DADLXX010000000
VERSION     DADLXX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.379"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..502
                     /locus_tag="JNX27_004362"
     CDS             <1..502
                     /locus_tag="JNX27_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7885266.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          149 a          109 c          153 g          102 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg cta
//
LOCUS       DADLXX010000373          473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLXX010000373 DADLXX010000000
VERSION     DADLXX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.380"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..410)
                     /locus_tag="JNX27_004363"
     CDS             complement(<1..410)
                     /locus_tag="JNX27_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY7885267.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          110 a          117 c           94 g          152 t
ORIGIN      
        1 tcatgttcga acaccactcc aacatctgtt tgaacacctg ttaaatttct ctcttgtttt
       61 gtttgaacat ctgcttcctt tctgcttctt ctggcctgaa cagatgcttt tcctgcggct
      121 gattttttgg ttaatttttc tctgacagat gccagatctt cctcaatccg aagatgcatc
      181 cattcgtcgc cgttatcaca aaaaaactcc cgcaaggatg gttcaacatc aacccatcgc
      241 tcgttagtca gacgggcaat ttttgccaac ctgtttttgg gtattggctt tcctgtttgc
      301 cagtaattga acatcagcaa caaatacgcg ccgtgctcct ctgctgacaa atgcatggtg
      361 tcagccagat aatcagctat gtacagttgc atgtatggta atgcggccat aattgccccg
      421 tatgatgcta cccggttgct tagaataagt gaggtagcct gagtttaacg gac
//
LOCUS       DADLXX010000374          464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLXX010000374 DADLXX010000000
VERSION     DADLXX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.381"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>464
                     /locus_tag="JNX27_004364"
     CDS             <1..>464
                     /locus_tag="JNX27_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7885268.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DADLXX010000375          459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLXX010000375 DADLXX010000000
VERSION     DADLXX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.382"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>459)
                     /locus_tag="JNX27_004365"
     rRNA            complement(<1..>459)
                     /locus_tag="JNX27_004365"
                     /product="23S ribosomal RNA"
BASE COUNT          100 a          149 c          106 g          104 t
ORIGIN      
        1 cctcacggtt cattagtacc ggttagctca acgcatcgct gcgcttacac acccggccta
       61 tcaacgtcgt cgtcttcaac gttccttcag gacccttaaa gggtcaggga gaactcatct
      121 cggggcaagt ttcgtgctta gatgctttca gcacttatct cttccgcatt tagctaccgg
      181 gcagtgccat tggcatgaca acccgaacac cagtgatgcg tccactccgg tcctctcgta
      241 ctaggagcag cccccctcag ttctccagcg cccacggcag atagggaccg aactgtctca
      301 cgacgttcta aacccagctc gcgtaccact ttaaatggcg aacagccata cccttgggac
      361 ctacttcagc cccaggatgt gatgagccga catcgaggtg ccaaacaccg ccgtcgatat
      421 gaactcttgg gcggtatcag cctgttatcc ccggagtac
//
LOCUS       DADLXX010000376          441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLXX010000376 DADLXX010000000
VERSION     DADLXX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.383"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            211..>441
                     /locus_tag="JNX27_004366"
     CDS             211..>441
                     /locus_tag="JNX27_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7885269.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          105 c           62 g          135 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccttggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg a
//
LOCUS       DADLXX010000377          427 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLXX010000377 DADLXX010000000
VERSION     DADLXX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.384"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(9..299)
                     /locus_tag="JNX27_004367"
     CDS             complement(9..299)
                     /locus_tag="JNX27_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY7885270.1"
                     /translation="MNAHWSSKKSNFLRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(268..>427)
                     /locus_tag="JNX27_004368"
     CDS             complement(268..>427)
                     /locus_tag="JNX27_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7885271.1"
                     /translation="YIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRE
                     RSLEFEKE"
BASE COUNT          130 a          108 c           78 g          111 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gagaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatatag
//
LOCUS       DADLXX010000378          426 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLXX010000378 DADLXX010000000
VERSION     DADLXX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.385"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..426
                     /locus_tag="JNX27_004369"
                     /pseudo
     CDS             1..426
                     /locus_tag="JNX27_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a          111 c          118 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aacgga
//
LOCUS       DADLXX010000379          424 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLXX010000379 DADLXX010000000
VERSION     DADLXX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.386"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(269..>424)
                     /locus_tag="JNX27_004370"
     CDS             complement(269..>424)
                     /locus_tag="JNX27_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7885272.1"
                     /translation="LRLDQTGRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAI
                     QERHRHD"
BASE COUNT          119 a           99 c           90 g          116 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcag
//
LOCUS       DADLXX010000380          420 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLXX010000380 DADLXX010000000
VERSION     DADLXX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.387"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>420
                     /locus_tag="JNX27_004371"
     CDS             <1..>420
                     /locus_tag="JNX27_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7885273.1"
                     /translation="ERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFG
                     NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
                     YISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRS"
BASE COUNT          104 a          102 c          133 g           81 t
ORIGIN      
        1 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
       61 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      121 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      181 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      241 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      301 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      361 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctgggt tatcgctcgc
//
LOCUS       DADLXX010000381          416 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLXX010000381 DADLXX010000000
VERSION     DADLXX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.388"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            43..>416
                     /locus_tag="JNX27_004372"
     CDS             43..>416
                     /locus_tag="JNX27_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7885274.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DADLXX010000382          413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLXX010000382 DADLXX010000000
VERSION     DADLXX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.389"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT          144 a           69 c           58 g          142 t
ORIGIN      
        1 accggaaagt atgcagacta tctgccatta taccgccaaa gtgatctact attccacaca
       61 gctatttagc tctctttttg ttgagctccc atgattcttt catttattgt tactaattat
      121 tttatgttat catgatgtta gaacacgctc tgctctgtgt ctctatttta tctatcatat
      181 tctgaggatt aactacataa caaggcgcaa gatatatatc caaaaacaaa taataccgtt
      241 gcaataattc ctttcagtaa aggaggtgtg attaatttca ggggatttca gagtaaaaat
      301 tgtacaacat attaaaatat gttcatttcg ttataataca cacaagaaaa tattaagtta
      361 gataatatat tctaaataac catagataaa aacgcacata atggggaata ttt
//
LOCUS       DADLXX010000383          400 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLXX010000383 DADLXX010000000
VERSION     DADLXX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.390"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            112..400
                     /locus_tag="JNX27_004373"
                     /pseudo
     CDS             112..400
                     /locus_tag="JNX27_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          103 a          120 c          104 g           73 t
ORIGIN      
        1 cgaccacaaa ctctgaccac aatctgccgg ttacgctgaa tctgctgaat cagaacttta
       61 cgcccacagc acctaatcag gtctgggtcg cagattcagt tgtccaggcc ttccgaaatc
      121 aaccgactga aggcgctggt cgcgaaactg cagcgtatgc agttcggtaa aagctcagaa
      181 aaacttcgcg caaaaaccga acggcggata caggaagcac aggagcgaat cagcgcactt
      241 caggaggaaa tggcggaaac actgggtgag caatatgacc cggtactgcc atcttccctg
      301 cgccagtctt cagcccgtaa accgttacca gcctcacttc cccgtgcccc ccgggtcatc
      361 cggccggaag aggaatgctg tccggcctgt ggtggtgaac
//
LOCUS       DADLXX010000384          396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLXX010000384 DADLXX010000000
VERSION     DADLXX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.391"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>396)
                     /locus_tag="JNX27_004374"
     rRNA            complement(<1..>396)
                     /locus_tag="JNX27_004374"
                     /product="23S ribosomal RNA"
BASE COUNT           74 a          124 c           88 g          110 t
ORIGIN      
        1 tattctctac ctgaccacct gtgtcggttt ggggtacgat ttgatgttac ctgatgctta
       61 gaggcttttc ctggaagcag ggcatttgtt gcttcagcac cgtagtgcct cgtcatcacg
      121 cctcggcctt gattttccgg atttgcctgg aaaaccagcc tacacgctta aaccgggaca
      181 accgtcgccc ggccaacata gccttctccg tccccccttc gcagtaacac caagtacagg
      241 aatattaacc tgtttcccat cgactacgcc tttcggcctc gccttagggg tcgactcacc
      301 ctgccccgat taacgttgga caggaaccct tggtcttccg gcgagcgggc ttttcacccg
      361 ctttatcgtt acttatgtca gcattcgcac ttctga
//
LOCUS       DADLXX010000385          389 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLXX010000385 DADLXX010000000
VERSION     DADLXX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.392"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>389
                     /locus_tag="JNX27_004375"
     CDS             <1..>389
                     /locus_tag="JNX27_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7885275.1"
                     /translation="ARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTY
                     TGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQEL"
BASE COUNT           75 a          116 c          115 g           83 t
ORIGIN      
        1 gcccgctact gggatgtgga gctgcgtgag atccctatgc gccccggtca gttgtttatg
       61 gacccgaaac gcatgattga agcctgtgac gaaaacacca tcggcgtggt gccgactttc
      121 ggcgtgacct acaccggtaa ctatgagttc ccacaaccgc tgcacgatgc gctggataaa
      181 ttccaggccg acaccggtat cgacatcgac atgcacatcg acgctgccag cggtggcttc
      241 ctggcaccgt tcgtcgcccc ggatatcgtc tgggacttcc gcctgccgcg tgtgaaatcg
      301 atcagtgctt caggccataa attcggtctg gctccgctgg gctgcggctg ggttatctgg
      361 cgtgacgaag aagcgctgcc gcaggaact
//
LOCUS       DADLXX010000386          388 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLXX010000386 DADLXX010000000
VERSION     DADLXX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.393"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(273..>388)
                     /locus_tag="JNX27_004376"
     CDS             complement(273..>388)
                     /locus_tag="JNX27_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY7885276.1"
                     /translation="KANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          107 a           89 c           76 g          116 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtgcg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattatca
      361 agcatagtgt ccccactatt tgcttttt
//
LOCUS       DADLXX010000387          386 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLXX010000387 DADLXX010000000
VERSION     DADLXX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.394"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>386
                     /locus_tag="JNX27_004377"
     CDS             <1..>386
                     /locus_tag="JNX27_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7885277.1"
                     /translation="IELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQ
                     MIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
                     RRGRAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          118 a           74 c          119 g           75 t
ORIGIN      
        1 aatcgaactg ggatacaggg agataaaaca gacgatgcaa ctgagcaggc tgacgctgag
       61 aagtaaaaag ccggagcttg tggagcaaga gctgtggggt gtcttactgg cttataatct
      121 ggtgagatat cagatgatta aaatggcgga acatctgaaa ggttactggc cgaatcaact
      181 gagtttctca gaatcatgcg gaatggtgat gagaatgctg atgacattgc agggcgcttc
      241 accgggacgt ataccggagc tgatgcgcga tcttgcaagt atgggacaac ttgtgaaatt
      301 accgacgaga aggggaaggg ccttcccgag agtggtaaag gagaggccct ggaaataccc
      361 cacagccccg aaaaagagcc agtcag
//
LOCUS       DADLXX010000388          380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLXX010000388 DADLXX010000000
VERSION     DADLXX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.395"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>380
                     /locus_tag="JNX27_004378"
     CDS             <1..>380
                     /locus_tag="JNX27_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7885278.1"
                     /translation="LQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQ
                     TVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRT
                     AARLLTEVACRAFASAAHLAAYAG"
BASE COUNT           75 a          129 c          100 g           76 t
ORIGIN      
        1 ttctccagcg atatccctca ccagaaaaac tcgcttcgct gggtgagaag aagctggcag
       61 cccagctctg caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac atagctcagg
      121 cactggccga acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta gtactgccac
      181 gtctggcact ccagctcatc acgctgcgta agcaaagaga cgaggtggcg cttgaggtag
      241 aacagcgagt tcttgctcac cctctttacc cggtcctgac cagtatgccc ggagtcggtg
      301 tcaggaccgc agccagactc ctcaccgagg tcgcctgccg cgccttcgcc tctgccgcac
      361 atctcgctgc ttatgctggc
//
LOCUS       DADLXX010000389          370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLXX010000389 DADLXX010000000
VERSION     DADLXX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.396"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..222
                     /locus_tag="JNX27_004379"
     CDS             <1..222
                     /locus_tag="JNX27_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7885279.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..>370
                     /locus_tag="JNX27_004380"
     CDS             268..>370
                     /locus_tag="JNX27_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7885280.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSN"
BASE COUNT          114 a           80 c           88 g           88 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaaca
//
LOCUS       DADLXX010000390          365 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLXX010000390 DADLXX010000000
VERSION     DADLXX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.397"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT          129 a           46 c           62 g          128 t
ORIGIN      
        1 gagaactgaa acagtgacag cactgaaaaa aatcttcttc aagttcatgt aaaaatcctt
       61 atttcctccg accagcgatc aatgccggta gagttgtcca ttgtattatt ggtgagctat
      121 gttgctttac attaatgaca gtgatgtgtg tcgattaaat attgttttaa gcattcatca
      181 taaaaatgta tcattcgttt caaaagatgt ctgtgtttgt atgaatatat tatttttcgt
      241 ggattctaat gatgattatt aataaatgtc aaatatgaaa gaaaccatgt ctgggaaaaa
      301 ttttacagat aattgatttt gtgaatatga tgattaaaat ttcacaatta aatccgaaaa
      361 aaaga
//
LOCUS       DADLXX010000391          359 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLXX010000391 DADLXX010000000
VERSION     DADLXX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.398"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>359
                     /locus_tag="JNX27_004381"
     rRNA            <1..>359
                     /locus_tag="JNX27_004381"
                     /product="23S ribosomal RNA"
BASE COUNT           97 a           76 c          106 g           80 t
ORIGIN      
        1 accttttgta taatgggtca gcgacttata ttctgtagca aggttaaccg aataggggag
       61 ccgaagggaa accgagtctt aactgggcgt taagttgcag ggtatagacc cgaaacccgg
      121 tgatctagcc atgggcaggt tgaaggttgg gtaacactaa ctggaggacc gaaccgacta
      181 atgttgaaaa attagcggat gacttgtggc tgggggtgaa aggccaatca aaccgggaga
      241 tagctggttc tccccgaaag ctatttaggt agcgcctcgt gaattcatct ccgggggtag
      301 agcactgttt cggcaagggg gtcatcccga cttaccaacc cgatgcaaac tgcgaatac
//
LOCUS       DADLXX010000392          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLXX010000392 DADLXX010000000
VERSION     DADLXX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.399"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..230)
                     /locus_tag="JNX27_004382"
     CDS             complement(<1..230)
                     /locus_tag="JNX27_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7885281.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHL"
BASE COUNT          128 a           55 c           53 g          115 t
ORIGIN      
        1 aggtgaatag tgaaaggttc agccaaagat ttctgatcgc ttttcagtac ggtatctgct
       61 acttcaccca gatttacagt taaatcttcg tcacctggtt taatagaaca tggagcagag
      121 ataacttcac ctttaaattt aacaataccg tgaccttcat cagcaaacac gttattggaa
      181 agaatcattg ctacaccagc aacaagaagt gactttttaa aagcagacat aatattcctt
      241 tatataaaga taagtaaaaa agagaatgat tagtaactgt tatttaagga ctaccaatca
      301 ttaaaatatt atattttgtt ttgtgatttt tataaaatta gtttatctaa a
//
LOCUS       DADLXX010000393          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLXX010000393 DADLXX010000000
VERSION     DADLXX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.denovo.400"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..208)
                     /locus_tag="JNX27_004383"
     CDS             complement(<1..208)
                     /locus_tag="JNX27_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7885282.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JNX27_004384"
     CDS             complement(205..>351)
                     /locus_tag="JNX27_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7885283.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADLXX010000394        17423 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.guided.082, whole genome shotgun sequence.
ACCESSION   DADLXX010000394 DADLXX010000000
VERSION     DADLXX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.guided.082"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="JNX27_004385"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="JNX27_004385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7885284.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="JNX27_004386"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="JNX27_004386"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7885285.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="JNX27_004387"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="JNX27_004387"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7885286.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            3030..6341
                     /gene="mscM"
                     /locus_tag="JNX27_004388"
     CDS             3030..6341
                     /gene="mscM"
                     /locus_tag="JNX27_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7885287.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNX27_004389"
     CDS             complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNX27_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY7885288.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNX27_004390"
     CDS             complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNX27_004390"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7885289.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9515..11323
                     /gene="frdA"
                     /locus_tag="JNX27_004391"
     CDS             9515..11323
                     /gene="frdA"
                     /locus_tag="JNX27_004391"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7885290.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            11316..12050
                     /locus_tag="JNX27_004392"
     CDS             11316..12050
                     /locus_tag="JNX27_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7885291.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12061..12456
                     /gene="frdC"
                     /locus_tag="JNX27_004393"
     CDS             12061..12456
                     /gene="frdC"
                     /locus_tag="JNX27_004393"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7885292.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12467..12826
                     /gene="frdD"
                     /locus_tag="JNX27_004394"
     CDS             12467..12826
                     /gene="frdD"
                     /locus_tag="JNX27_004394"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7885293.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12889..14022
                     /gene="blaEC"
                     /locus_tag="JNX27_004395"
     CDS             12889..14022
                     /gene="blaEC"
                     /locus_tag="JNX27_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7885294.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            14111..14644
                     /locus_tag="JNX27_004396"
     CDS             14111..14644
                     /locus_tag="JNX27_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7885295.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14641..14870)
                     /locus_tag="JNX27_004397"
                     /pseudo
     CDS             complement(14641..14870)
                     /locus_tag="JNX27_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNX27_004398"
     CDS             complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNX27_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7885296.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNX27_004399"
     CDS             complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNX27_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7885297.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNX27_004400"
     CDS             complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNX27_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7885298.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16088..17116
                     /gene="epmB"
                     /locus_tag="JNX27_004401"
     CDS             16088..17116
                     /gene="epmB"
                     /locus_tag="JNX27_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7885299.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4338 a         4568 c         4487 g         4030 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaggccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggg cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggagc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt atggcgctga
     4861 tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg ctttgcttta
     4921 tcctcatttg cggtgcgctg gcggtggtca ccctaagcct gaaaaaagcc ggtattccgt
     4981 tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg tggaacatga
     5041 tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg gcaacggcgc
     5101 aggcactgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
     5161 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
     5221 agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg cgaagaggaa gcgcatcatc
     5281 acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc gatgccatca
     5341 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg cgttcggtca
     5401 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tcgctgtggg
     5461 atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc ggtgcggtgc
     5521 tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg cccgcgctgc
     5581 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
     5641 ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
     5701 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
     5761 tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa aaaccgattc
     5821 gcattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa attaacaccc
     5881 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
     5941 ttaccgcgca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta gtgttgacga
     6001 ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc accgcagcgc
     6061 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
     6121 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
     6181 tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt atcgatatgc
     6241 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
     6301 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag ctgccaaatg
     6361 atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg gccctggaga
     6421 gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat gagttgcagg
     6481 atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact gcaatagttc
     6541 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
     6601 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
     6661 accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
     6721 aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc gaggaacagg
     6781 taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg
     6841 gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca ctgcatccac
     6901 atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct
     6961 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
     7021 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
     7081 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
     7141 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
     7201 ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
     7261 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
     7321 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
     7381 gactgataac ccgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca
     7441 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
     7501 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
     7561 accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca
     7621 atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg tacccaaact
     7681 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
     7741 aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc ttcttctttg
     7801 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
     7861 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
     7921 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
     7981 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
     8041 ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga taccttgatg
     8101 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
     8161 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
     8221 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
     8281 gacgatcaac acctaatgcc acgccggagc agtcaggcat accgactttc aaggcttcaa
     8341 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
     8401 gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct
     8461 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
     8521 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttaccaa
     8581 tatttgcctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg
     8641 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
     8701 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
     8761 ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
     8821 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
     8881 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca gcaaccagca
     8941 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
     9001 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
     9061 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
     9121 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
     9181 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
     9241 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
     9301 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
     9361 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
     9421 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
     9481 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
     9541 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
     9601 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
     9661 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
     9721 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
     9781 ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga
     9841 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc
     9901 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
     9961 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    10021 cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt
    10081 cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    10141 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    10201 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    10261 tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    10321 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    10381 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg
    10441 tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct
    10501 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    10561 tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    10621 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    10681 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    10741 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    10801 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    10861 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    10921 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    10981 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    11041 gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    11101 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
    11161 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
    11221 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    11281 taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    11341 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    11401 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    11461 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
    11521 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacagta
    11581 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    11641 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    11701 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    11761 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    11821 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    11881 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
    11941 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    12001 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    12061 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    12121 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    12181 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    12241 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
    12301 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    12361 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    12421 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    12481 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    12541 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    12601 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    12661 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    12721 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
    12781 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
    12841 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    12901 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    12961 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    13021 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    13081 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    13141 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
    13201 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    13261 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
    13321 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
    13381 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    13441 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
    13501 attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca
    13561 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
    13621 gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt
    13681 caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
    13741 ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac
    13801 aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta
    13861 cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt
    13921 attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagag
    13981 agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa
    14041 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
    14101 ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
    14161 tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa
    14221 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
    14281 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
    14341 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
    14401 cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa
    14461 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
    14521 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
    14581 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
    14641 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag
    14701 acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc
    14761 gccaataccc gtccacacgg catcaaacgg ctaaagccgt gggtatattt caggccaacg
    14821 gcccatacca cttccggcag accagcaata actaagataa tccaggacat atcaggctcc
    14881 ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat
    14941 ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt tatgacgatc
    15001 gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt gctgcgcttt
    15061 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
    15121 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
    15181 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
    15241 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga agagaagaaa
    15301 aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg
    15361 caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
    15421 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
    15481 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt
    15541 tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagccgg tttgccgcca
    15601 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag
    15661 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
    15721 tccagcagcc atttagcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
    15781 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
    15841 tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta
    15901 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
    15961 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
    16021 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
    16081 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
    16141 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
    16201 cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
    16261 atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac
    16321 ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccactgg aagaacagca
    16381 cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
    16441 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
    16501 gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga
    16561 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
    16621 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
    16681 gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc gttctacgct
    16741 gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa cgttccgtca
    16801 ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg ttctgttacg
    16861 tggtgtgaac gataacgcac aaacgttggc aaacctgagt aatgcgttgt tcgatgccgg
    16921 cgtgatgccg tattacctgc atgtgctaga taaagtacag ggcgcggcgc attttatggt
    16981 gagtgatgac gaagcacggc agattatgcg tgagttgctg acattggtgt cgggatatct
    17041 ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca
    17101 gctacgccag cagtaactga ttaaacaggg aagttaaaca ttaaaattgc ctgatgcgct
    17161 acgcttatca ggcctacatc gtgcattgaa attgaatgga tttgcaatga actgtaggcc
    17221 ggataaggcg tttacgccgc atccggcaag agcaacaaac gcaagtacag caacctgtca
    17281 ttaaactgag aaatttaatc tttgcacgtt gcataacctt taattattca tatgcattgc
    17341 tgttgttact ttttatatag ctaacaccaa taattaacct ttcttttcgg taatgactcc
    17401 aacttactga tagtgtttta tgt
//
LOCUS       DADLXX010000395        10830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.guided.130, whole genome shotgun sequence.
ACCESSION   DADLXX010000395 DADLXX010000000
VERSION     DADLXX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.guided.130"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            59..1103
                     /locus_tag="JNX27_004402"
                     /pseudo
     CDS             59..1103
                     /locus_tag="JNX27_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1219..1392
                     /locus_tag="JNX27_004403"
     CDS             1219..1392
                     /locus_tag="JNX27_004403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7885300.1"
                     /translation="MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDN
                     AWALSESWCGTIQ"
     gene            1441..1779
                     /locus_tag="JNX27_004404"
                     /pseudo
     CDS             1441..1779
                     /locus_tag="JNX27_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNX27_004405"
     CDS             complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNX27_004405"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7885301.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3555..4013
                     /gene="gpt"
                     /locus_tag="JNX27_004406"
     CDS             3555..4013
                     /gene="gpt"
                     /locus_tag="JNX27_004406"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7885302.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4105..5349
                     /gene="frsA"
                     /locus_tag="JNX27_004407"
     CDS             4105..5349
                     /gene="frsA"
                     /locus_tag="JNX27_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7885303.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5407..5808
                     /locus_tag="JNX27_004408"
     CDS             5407..5808
                     /locus_tag="JNX27_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7885304.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="JNX27_004409"
     CDS             complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="JNX27_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7885305.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7190..8293
                     /gene="proB"
                     /locus_tag="JNX27_004410"
     CDS             7190..8293
                     /gene="proB"
                     /locus_tag="JNX27_004410"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7885306.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8305..9558
                     /gene="proA"
                     /locus_tag="JNX27_004411"
     CDS             8305..9558
                     /gene="proA"
                     /locus_tag="JNX27_004411"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7885307.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9673..9748
                     /locus_tag="JNX27_004412"
     tRNA            9673..9748
                     /locus_tag="JNX27_004412"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9706..9708,aa:Thr,seq:cgt)
     gene            complement(9763..>10830)
                     /locus_tag="JNX27_004413"
     CDS             complement(9763..>10830)
                     /locus_tag="JNX27_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7885308.1"
                     /translation="TLTRWPVFVDHFKAWRVKNLGESPKKLFKEACIRWLREKSDKKS
                     IDDDKSIISFWMLHFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPV
                     PEYKPKLASKGTKTRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESK
                     RLFAEIAPHFFPVVMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVP
                     LNETACQILRKQQGLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGI
                     SNFRFHDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDA
                     IISRNGTNTAQEENVVYLNAR"
BASE COUNT         2582 a         2705 c         2828 g         2715 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggtcc atagcggctc gagggagcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca ggcccgtcag gagtgaggaa acattgcatt
      901 cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
      961 cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
     1021 ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa agtgtggtgc
     1081 aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
     1141 caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc gcaggggccg
     1201 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
     1261 caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga cacgctgcgt
     1321 tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag ctggtgtggc
     1381 actattcagt agatttgccc gagcccgtat cgcccacatc acgggcgtaa aaactggttt
     1441 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1501 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1561 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag ccagcatgct
     1621 aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca ataggaaaat
     1681 agcgcgtagc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1741 cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1801 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1861 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1921 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1981 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     2041 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     2101 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     2161 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     2221 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac cacacctttt
     2281 gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     2341 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     2401 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2461 gatttcagtg cgtcgacttt atcagctgtg acagcaatgg tcgcaaaggc ttcacgcggg
     2521 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2581 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2641 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2701 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2761 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2821 ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt cagcagcact
     2881 tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2941 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     3001 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     3061 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     3121 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     3181 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt
     3241 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     3301 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     3361 caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc gcgtcaggga
     3421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagata
     3481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     3541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     3601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     3661 gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3721 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3781 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3841 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     3901 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     3961 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4021 aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca
     4081 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     4141 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggtgcacaa
     4201 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
     4261 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
     4321 attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt gattggctac
     4381 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
     4441 tgtacggaag aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac
     4501 aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta
     4561 tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag
     4621 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
     4681 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
     4741 ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccgtcg
     4801 gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc
     4861 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
     4921 cgtttcggcg ctaacgttgc cgtgcgtttg gcataccttg aatcgccgcg tctgaaagcg
     4981 gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag
     5041 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac
     5101 gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt
     5161 cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag
     5221 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
     5281 ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc
     5341 ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag
     5401 atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca
     5461 ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg
     5521 gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg ctggtggatg
     5581 gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct gttcgataaa
     5641 gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg actggagcac
     5701 accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg
     5761 gaaccggcgg atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt gaaatgtgcc
     5821 ggatgcatca catccggcaa tattcattaa aactgatacg tcatcccaac cgcgacagta
     5881 tcatcattat taatattcag cttattatcg ctatccagtt tgttgatttt ataatcaaca
     5941 aacgctgaca tatttttgtt gaaataatac gtagcaccga cgtcgatata attgaccaga
     6001 tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc cagcgatgga
     6061 cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt cttattggca
     6121 aagccgccag ttattggcgt cattttgcgt gtttcagaat agaaagttgc cagataaata
     6181 ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc tgtgccacgg
     6241 ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact aatggcgaaa
     6301 tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta
     6361 acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc atcgataacg
     6421 ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt cataaagttg
     6481 tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca ggcttccacg
     6541 tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt atatttcaac
     6601 ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt atttccggca
     6661 aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgagt ttcaccttta
     6721 aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact catataatgc
     6781 atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata tatttctgca
     6841 gcctgtacag atgcagatgc cacaatgccc attaccacta atgccagagt gctctttttc
     6901 attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc cgttgaaaac
     6961 aggaaagttt ttaacctgag attgttaaag atatattaca gattaatgat attctgaaaa
     7021 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc
     7081 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
     7141 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
     7201 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
     7261 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
     7321 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
     7381 actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat
     7441 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
     7501 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
     7561 gctcgatcac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
     7621 taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
     7681 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca gcaatccgca
     7741 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
     7801 cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg ctgacgtggc
     7861 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
     7921 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
     7981 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     8041 aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     8101 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     8161 ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca
     8221 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     8281 gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     8341 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     8401 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     8461 gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     8521 ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag
     8581 gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     8641 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     8701 tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg tcgcactaac
     8761 gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc ggcgggtgcc
     8821 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     8881 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     8941 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagc
     9001 gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     9061 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     9121 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca
     9181 cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag
     9241 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     9301 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     9361 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     9421 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     9481 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     9541 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     9601 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     9661 tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc
     9721 gtaggttcga ctcctattat cggcaccatt taaatcaata agttacctcg catttaagta
     9781 aaccacgttc tcctcttgtg ccgtatttgt gccattgcga cttataatcg catcgatttt
     9841 gcttgcgtgc tcggtgagat gcccggctga aaggtgggcg tatctttgaa ccatttcgag
     9901 agtttcccat cctcccatct ctttaagtgc aagaagagag acaccggact gaaccagcca
     9961 gcttgcccag gtatgcctca gatcatggaa gcggaagttg ctaatgcctg cccgctttaa
    10021 cgctcccttc catgccttgt tgctgtcggt tctcatcttc cttaccgctg ctgtttttgt
    10081 tccgtcgctt cggtaggcag gtttggtgtg gacaaatacc catctcttat ggagcccctg
    10141 ctgttttctt aatatctggc atgcggtttc gttaagagga actccgatcg cattgccagc
    10201 ttttgtttca tcagggtgca tccatgccat tttcttatcc agatcgacct gtgaccactc
    10261 aaggtctgta acgttggaac ggcgaaggcc tgtcgtgatt gcaaacatga ccacagggaa
    10321 gaagtgagga gcaatttctg caaacaggcg cttcgattcc tcctctgtaa gccatctgat
    10381 gcgtccattc ttaacgcgtg gtgttgatat tttgggtgcc ctgtcaagcc atccccattc
    10441 aacagccata ttgagaatgg cgcgaagtat tgccagatgc ctcgtcttcg ttcctttgct
    10501 tgcaagcttt ggtttatact ccggcaccgg attgccaagt cgcaaacacc tgtcccggct
    10561 catctcccag ttcaggcgat ggcggcggtt ttccatcccg tctaccgcct ccattatttt
    10621 ttctgttgtt atgtcagaga gaatggcttc tctgaagtgc aacatccaga acgatataat
    10681 gctcttgtca tcatcaatgg acttcttatc cgatttctca cgcagccacc gtatgcaggc
    10741 ttccttgaat agctttttcg gtgattcccc gagatttttt actctccacg ctttgaagtg
    10801 gtcaacaaaa actggccacc gagttagagt
//
LOCUS       DADLXX010000396         8853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.guided.150, whole genome shotgun sequence.
ACCESSION   DADLXX010000396 DADLXX010000000
VERSION     DADLXX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.guided.150"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            264..923
                     /locus_tag="JNX27_004414"
     CDS             264..923
                     /locus_tag="JNX27_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7885309.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JNX27_004415"
     CDS             complement(1124..1501)
                     /locus_tag="JNX27_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7885310.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="JNX27_004416"
     CDS             complement(1568..4534)
                     /locus_tag="JNX27_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY7885311.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="JNX27_004417"
     CDS             complement(4537..5097)
                     /locus_tag="JNX27_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7885312.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="JNX27_004418"
     CDS             complement(5223..5573)
                     /locus_tag="JNX27_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY7885313.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNX27_004419"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNX27_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7885314.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6999..7829
                     /locus_tag="JNX27_004420"
     CDS             6999..7829
                     /locus_tag="JNX27_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY7885315.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7942..8733
                     /gene="aadA1"
                     /locus_tag="JNX27_004421"
     CDS             7942..8733
                     /gene="aadA1"
                     /locus_tag="JNX27_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7885316.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2016 a         2367 c         2497 g         1973 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6961 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     7021 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7081 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7141 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7201 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7261 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7321 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7381 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7441 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7501 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7561 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7621 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7681 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7741 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7801 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7861 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7921 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8641 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     8701 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     8761 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgcggtgatg
     8821 ctgccaactt actgatttag tgtatgatgg tgt
//
LOCUS       DADLXX010000397         5665 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.guided.182, whole genome shotgun sequence.
ACCESSION   DADLXX010000397 DADLXX010000000
VERSION     DADLXX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.guided.182"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            70..285
                     /locus_tag="JNX27_004422"
     CDS             70..285
                     /locus_tag="JNX27_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7885317.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="JNX27_004423"
     CDS             278..664
                     /locus_tag="JNX27_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7885318.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="JNX27_004424"
     CDS             672..1358
                     /locus_tag="JNX27_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7885319.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNX27_004425"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNX27_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7885320.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNX27_004426"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNX27_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7885321.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNX27_004427"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNX27_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7885322.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4040..4450
                     /locus_tag="JNX27_004428"
                     /pseudo
     CDS             4040..4450
                     /locus_tag="JNX27_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4466..>5665)
                     /locus_tag="JNX27_004429"
     CDS             complement(4466..>5665)
                     /locus_tag="JNX27_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY7885323.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1568 a         1045 c         1230 g         1822 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa
     4321 aactatcctt gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     5281 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     5341 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     5401 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     5461 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     5521 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     5581 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc
     5641 aacttactga tagtgtttta tgttc
//
LOCUS       DADLXX010000398         4226 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.guided.216, whole genome shotgun sequence.
ACCESSION   DADLXX010000398 DADLXX010000000
VERSION     DADLXX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.guided.216"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            110..1015
                     /gene="mph(A)"
                     /locus_tag="JNX27_004430"
     CDS             110..1015
                     /gene="mph(A)"
                     /locus_tag="JNX27_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAY7885324.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /locus_tag="JNX27_004431"
     CDS             1012..2250
                     /locus_tag="JNX27_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7885325.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /locus_tag="JNX27_004432"
     CDS             2250..2834
                     /locus_tag="JNX27_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7885326.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="JNX27_004433"
     CDS             complement(3327..4091)
                     /locus_tag="JNX27_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAY7885327.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          719 a         1312 c         1317 g          878 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcaggca ctgttgcaaa
     4201 gttagcgatg aggcagcctt ttgtct
//
LOCUS       DADLXX010000399         1817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.guided.281, whole genome shotgun sequence.
ACCESSION   DADLXX010000399 DADLXX010000000
VERSION     DADLXX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.guided.281"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            112..207
                     /locus_tag="JNX27_004434"
     CDS             112..207
                     /locus_tag="JNX27_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAY7885328.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="JNX27_004435"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="JNX27_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAY7885329.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          663 a          303 c          297 g          554 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atggtgt
//
LOCUS       DADLXX010000400         1704 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 414/13
            SAMEA2810806-rid11413033.guided.291, whole genome shotgun sequence.
ACCESSION   DADLXX010000400 DADLXX010000000
VERSION     DADLXX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810806
            Sequence Read Archive: ERR654269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941429.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941429.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:10:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,357
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 195 of 381
            Pseudo Genes (incomplete)         :: 89 of 381
            Pseudo Genes (internal stop)      :: 136 of 381
            Pseudo Genes (multiple problems)  :: 37 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810806-rid11413033.guided.291"
                     /strain="414/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            13..474
                     /locus_tag="JNX27_004436"
     CDS             13..474
                     /locus_tag="JNX27_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7885330.1"
                     /translation="MTTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRDTADM
                     IQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAK
                     LKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="JNX27_004437"
     CDS             657..1517
                     /locus_tag="JNX27_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7885331.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          455 a          374 c          437 g          438 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgc
//
